                         SEQUENCE LISTING

<110>  E. I. du Pont de Nemours & Co., Inc.
       Zhu, Quinn
       Xue, Zhixiong
 
<120>  PEROXISOME BIOGENESIS FACTOR PROTEIN (PEX) DISRUPTIONS FOR 
       ALTERING POLYUNSATURATED FATTY ACIDS AND TOTAL LIPID CONTENT IN 
       OLEAGINOUS EUKARYOTIC ORGANISMS

<130>  CL3847

<160>  89    

<170>  PatentIn version 3.4

<210>  1
<211>  1024
<212>  PRT
<213>  Yarrowia lipolytica CLIB122 (GenBank Accession No. CAG82178)


<220>
<221>  MISC_FEATURE
<222>  (1)..(1024)
<223>  YlPex1p

<400>  1

Met Thr Ser Lys Ser Asp Tyr Ser Gly Lys Asp Lys Ile Glu Leu Asp 
1               5                   10                  15      


Pro Val Phe Ala Lys Ser Ile Asp Leu Leu Pro Asn Thr Gln Val Val 
            20                  25                  30          


Ile Asp Ile Gln Leu Asn Pro Lys Ile Ala His Thr Ile His Leu Glu 
        35                  40                  45              


Pro Val Thr Val Ala Asp Trp Glu Ile Val Glu Leu His Ala Ala Tyr 
    50                  55                  60                  


Leu Glu Ser Arg Met Ile Asn Gln Val Arg Ala Val Ser Pro Asn Gln 
65                  70                  75                  80  


Pro Val Thr Val Tyr Pro Ser Ser Thr Thr Ser Ala Thr Leu Lys Val 
                85                  90                  95      


Ile Arg Ile Glu Pro Asp Leu Gly Ala Ala Gly Phe Ala Lys Leu Ser 
            100                 105                 110         


Pro Asp Ser Glu Val Val Val Ala Pro Lys Gln Arg Lys Lys Glu Glu 
        115                 120                 125             


Lys Gln Val Lys Lys Arg Ser Gly Ser Ala Arg Ser Thr Gly Ser Gln 
    130                 135                 140                 


Lys Arg Lys Gly Gly Arg Gly Pro His Ala Leu Arg Arg Ala Ile Ser 
145                 150                 155                 160 


Glu Asp Phe Asp Gly His Leu Arg Leu Glu Val Ser Leu Asp Val Ser 
                165                 170                 175     


Gln Leu Pro Pro Glu Phe His Gln Leu Lys Asn Val Ser Ile Lys Val 
            180                 185                 190         


Ile Thr Pro Pro Asn Leu Ala Ser Pro Gln Gln Ala Ala Ser Ile Ala 
        195                 200                 205             


Val Glu Glu Lys Ser Glu Glu Ser Leu Ser Gln Asn Lys Pro Pro Ser 
    210                 215                 220                 


Ser Glu Pro Lys Val Glu Val Pro Pro Asp Ile Ile Asn Pro Ala Ser 
225                 230                 235                 240 


Glu Ile Val Ala Thr Leu Val Asn Asp Thr Thr Ser Pro Thr Gly His 
                245                 250                 255     


Ala Lys Leu Ser Tyr Ala Leu Ala Asp Ala Leu Gly Ile Pro Ser Ser 
            260                 265                 270         


Val Gly His Val Ile Arg Phe Glu Ser Ala Ser Lys Pro Leu Ser Gln 
        275                 280                 285             


Lys Pro Gly Ala Leu Val Ile His Arg Phe Ile Thr Lys Thr Val Gly 
    290                 295                 300                 


Ala Ala Glu Gln Lys Ser Leu Arg Leu Lys Gly Glu Lys Asn Ala Asp 
305                 310                 315                 320 


Asp Gly Val Ser Ala Asp Asp Gln Phe Ser Leu Leu Glu Glu Leu Lys 
                325                 330                 335     


Lys Leu Gln Met Leu Glu Gly Pro Ile Thr Asn Phe Gln Arg Leu Pro 
            340                 345                 350         


Pro Ile Pro Glu Leu Leu Pro Leu Gly Gly Val Ile Gly Leu Gln Asn 
        355                 360                 365             


Ser Glu Gly Trp Ile Gln Gly Gly Tyr Leu Gly Glu Glu Pro Ile Pro 
    370                 375                 380                 


Phe Val Ser Gly Ser Glu Ile Leu Arg Ser Glu Ser Ser Leu Ser Pro 
385                 390                 395                 400 


Ser Asn Ile Glu Ser Glu Asp Lys Arg Val Val Gly Leu Asp Asn Met 
                405                 410                 415     


Leu Asn Lys Ile Asn Glu Val Leu Ser Arg Asp Ser Ile Gly Cys Leu 
            420                 425                 430         


Val Tyr Gly Ser Arg Gly Ser Gly Lys Ser Ala Val Leu Asn His Ile 
        435                 440                 445             


Lys Lys Glu Cys Lys Val Ser His Thr His Thr Val Ser Ile Ala Cys 
    450                 455                 460                 


Gly Leu Ile Ala Gln Asp Arg Val Gln Ala Val Arg Glu Ile Leu Thr 
465                 470                 475                 480 


Lys Ala Phe Leu Glu Ala Ser Trp Phe Ser Pro Ser Val Leu Phe Leu 
                485                 490                 495     


Asp Asp Ile Asp Ala Leu Met Pro Ala Glu Val Glu His Ala Asp Ser 
            500                 505                 510         


Ser Arg Thr Arg Gln Leu Thr Gln Leu Phe Leu Glu Leu Ala Leu Pro 
        515                 520                 525             


Ile Met Lys Ser Arg His Val Ser Val Val Ala Ser Ala Gln Ala Lys 
    530                 535                 540                 


Glu Ser Leu His Met Asn Leu Val Thr Gly His Val Phe Glu Glu Leu 
545                 550                 555                 560 


Phe His Leu Lys Ser Pro Asp Lys Glu Ala Arg Leu Ala Ile Leu Ser 
                565                 570                 575     


Glu Ala Val Lys Leu Met Asp Gln Asn Val Ser Phe Ser Gln Asn Asp 
            580                 585                 590         


Val Leu Glu Ile Ala Ser Gln Val Asp Gly Tyr Leu Pro Gly Asp Leu 
        595                 600                 605             


Trp Thr Leu Ser Glu Arg Ala Gln His Glu Met Ala Leu Arg Gln Ile 
    610                 615                 620                 


Glu Ile Gly Leu Glu Asn Pro Ser Ile Gln Leu Ala Asp Phe Met Lys 
625                 630                 635                 640 


Ala Leu Glu Asp Phe Val Pro Ser Ser Leu Arg Gly Val Lys Leu Gln 
                645                 650                 655     


Lys Ser Asn Val Lys Trp Asn Asp Ile Gly Gly Leu Lys Glu Thr Lys 
            660                 665                 670         


Ala Val Leu Leu Glu Thr Leu Glu Trp Pro Thr Lys Tyr Ala Pro Ile 
        675                 680                 685             


Phe Ala Ser Cys Pro Leu Arg Leu Arg Ser Gly Leu Leu Leu Tyr Gly 
    690                 695                 700                 


Tyr Pro Gly Cys Gly Lys Thr Tyr Leu Ala Ser Ala Val Ala Ala Gln 
705                 710                 715                 720 


Cys Gly Leu Asn Phe Ile Ser Ile Lys Gly Pro Glu Ile Leu Asn Lys 
                725                 730                 735     


Tyr Ile Gly Ala Ser Glu Gln Ser Val Arg Glu Leu Phe Glu Arg Ala 
            740                 745                 750         


Gln Ala Ala Lys Pro Cys Ile Leu Phe Phe Asp Glu Phe Asp Ser Ile 
        755                 760                 765             


Ala Pro Lys Arg Gly His Asp Ser Thr Gly Val Thr Asp Arg Val Val 
    770                 775                 780                 


Asn Gln Met Leu Thr Gln Met Asp Gly Ala Glu Gly Leu Asp Gly Val 
785                 790                 795                 800 


Tyr Val Leu Ala Ala Thr Ser Arg Pro Asp Leu Ile Asp Pro Ala Leu 
                805                 810                 815     


Leu Arg Pro Gly Arg Leu Asp Lys Met Leu Ile Cys Asp Leu Pro Ser 
            820                 825                 830         


Tyr Glu Asp Arg Leu Asp Ile Leu Arg Ala Ile Val Asp Gly Lys Met 
        835                 840                 845             


His Leu Asp Gly Glu Val Glu Leu Glu Tyr Val Ala Ser Arg Thr Asp 
    850                 855                 860                 


Gly Phe Ser Gly Ala Asp Leu Gln Ala Val Met Phe Asn Ala Tyr Leu 
865                 870                 875                 880 


Glu Ala Ile His Glu Val Val Asp Val Ala Asp Asp Thr Ala Ala Asp 
                885                 890                 895     


Thr Pro Ala Leu Glu Asp Lys Arg Leu Glu Phe Phe Gln Thr Thr Leu 
            900                 905                 910         


Gly Asp Ala Lys Lys Asp Pro Ala Ala Val Gln Asn Glu Val Met Asn 
        915                 920                 925             


Ala Arg Ala Ala Val Ala Glu Lys Ala Arg Val Thr Ala Lys Leu Glu 
    930                 935                 940                 


Ala Leu Phe Lys Gly Met Ser Val Gly Val Asp Asn Asp Asp Asp Lys 
945                 950                 955                 960 


Pro Arg Lys Lys Ala Val Val Val Ile Lys Pro Gln His Met Asn Lys 
                965                 970                 975     


Ser Leu Asp Glu Thr Ser Pro Ser Ile Ser Lys Lys Glu Leu Leu Lys 
            980                 985                 990         


Leu Lys Gly Ile Tyr Ser Gln Phe  Val Ser Gly Arg Ser  Gly Asp Met 
        995                 1000                 1005             


Pro Pro  Gly Thr Ala Ser Thr  Asp Val Gly Gly Arg  Ala Thr Leu 
    1010                 1015                 1020             


Ala 
    


<210>  2
<211>  381
<212>  PRT
<213>  Yarrowia lipolytica CLIB122 (GenBank Accession No. CAG77647)


<220>
<221>  MISC_FEATURE
<222>  (1)..(381)
<223>  YlPex2p

<400>  2

Met Ser Ser Val Leu Arg Leu Phe Lys Ile Gly Ala Pro Val Pro Asn 
1               5                   10                  15      


Val Arg Val His Gln Leu Asp Ala Ser Leu Leu Asp Ala Glu Leu Val 
            20                  25                  30          


Asp Leu Leu Lys Asn Gln Leu Phe Lys Gly Phe Thr Asn Phe His Pro 
        35                  40                  45              


Glu Phe Arg Asp Lys Tyr Glu Ser Glu Leu Val Leu Ala Leu Lys Leu 
    50                  55                  60                  


Ile Leu Phe Lys Leu Thr Val Trp Asp His Ala Ile Thr Tyr Gly Gly 
65                  70                  75                  80  


Lys Leu Gln Asn Leu Lys Phe Ile Asp Ser Arg His Ser Ser Lys Leu 
                85                  90                  95      


Gln Ile Gln Pro Ser Val Ile Gln Lys Leu Gly Tyr Gly Ile Leu Val 
            100                 105                 110         


Val Gly Gly Gly Tyr Leu Trp Ser Lys Ile Glu Gly Tyr Leu Leu Ala 
        115                 120                 125             


Arg Ser Glu Asp Asp Val Ala Thr Asp Gly Thr Ser Val Arg Gly Ala 
    130                 135                 140                 


Ser Ala Ala Arg Gly Ala Leu Lys Val Ala Asn Phe Ala Ser Leu Leu 
145                 150                 155                 160 


Tyr Ser Ala Ala Thr Leu Gly Asn Phe Val Ala Phe Leu Tyr Thr Gly 
                165                 170                 175     


Arg Tyr Ala Thr Val Ile Met Arg Leu Leu Arg Ile Arg Leu Val Pro 
            180                 185                 190         


Ser Gln Arg Thr Ser Ser Arg Gln Val Ser Tyr Glu Phe Gln Asn Arg 
        195                 200                 205             


Gln Leu Val Trp Asn Ala Phe Thr Glu Phe Leu Ile Phe Ile Leu Pro 
    210                 215                 220                 


Leu Leu Gln Leu Pro Lys Leu Lys Arg Arg Ile Glu Arg Lys Leu Gln 
225                 230                 235                 240 


Ser Leu Asn Val Thr Arg Val Gly Asn Val Glu Glu Ala Ser Glu Gly 
                245                 250                 255     


Glu Leu Ala His Leu Pro Gln Lys Thr Cys Ala Ile Cys Phe Arg Asp 
            260                 265                 270         


Glu Glu Glu Gln Glu Gly Gly Gly Gly Ala Ser His Tyr Ser Thr Asp 
        275                 280                 285             


Val Thr Asn Pro Tyr Gln Ala Asp Cys Gly His Val Tyr Cys Tyr Val 
    290                 295                 300                 


Cys Leu Val Thr Lys Leu Ala Gln Gly Asp Gly Asp Gly Trp Asn Cys 
305                 310                 315                 320 


Tyr Arg Cys Ala Lys Gln Val Gln Lys Met Lys Pro Trp Val Asp Val 
                325                 330                 335     


Asp Glu Ala Ala Val Val Gly Ala Ala Glu Met His Glu Lys Val Asp 
            340                 345                 350         


Val Ile Glu His Ala Glu Asp Asn Glu Gln Glu Glu Glu Glu Phe Asp 
        355                 360                 365             


Asp Asp Asp Glu Asp Ser Asn Phe Gln Leu Met Lys Asp 
    370                 375                 380     


<210>  3
<211>  431
<212>  PRT
<213>  Yarrowia lipolytica CLIB122 (GenBank Accession No. CAG78565)


<220>
<221>  MISC_FEATURE
<222>  (1)..(431)
<223>  YlPex3p

<400>  3

Met Asp Phe Phe Arg Arg His Gln Lys Lys Val Leu Ala Leu Val Gly 
1               5                   10                  15      


Val Ala Leu Ser Ser Tyr Leu Phe Ile Asp Tyr Val Lys Lys Lys Phe 
            20                  25                  30          


Phe Glu Ile Gln Gly Arg Leu Ser Ser Glu Arg Thr Ala Lys Gln Asn 
        35                  40                  45              


Leu Arg Arg Arg Phe Glu Gln Asn Gln Gln Asp Ala Asp Phe Thr Ile 
    50                  55                  60                  


Met Ala Leu Leu Ser Ser Leu Thr Thr Pro Val Met Glu Arg Tyr Pro 
65                  70                  75                  80  


Val Asp Gln Ile Lys Ala Glu Leu Gln Ser Lys Arg Arg Pro Thr Asp 
                85                  90                  95      


Arg Val Leu Ala Leu Glu Ser Ser Thr Ser Ser Ser Ala Thr Ala Gln 
            100                 105                 110         


Thr Val Pro Thr Met Thr Ser Gly Ala Thr Glu Glu Gly Glu Lys Ser 
        115                 120                 125             


Lys Thr Gln Leu Trp Gln Asp Leu Lys Arg Thr Thr Ile Ser Arg Ala 
    130                 135                 140                 


Phe Ser Leu Val Tyr Ala Asp Ala Leu Leu Ile Phe Phe Thr Arg Leu 
145                 150                 155                 160 


Gln Leu Asn Ile Leu Gly Arg Arg Asn Tyr Val Asn Ser Val Val Ala 
                165                 170                 175     


Leu Ala Gln Gln Gly Arg Glu Gly Asn Ala Glu Gly Arg Val Ala Pro 
            180                 185                 190         


Ser Phe Gly Asp Leu Ala Asp Met Gly Tyr Phe Gly Asp Leu Ser Gly 
        195                 200                 205             


Ser Ser Ser Phe Gly Glu Thr Ile Val Asp Pro Asp Leu Asp Glu Gln 
    210                 215                 220                 


Tyr Leu Thr Phe Ser Trp Trp Leu Leu Asn Glu Gly Trp Val Ser Leu 
225                 230                 235                 240 


Ser Glu Arg Val Glu Glu Ala Val Arg Arg Val Trp Asp Pro Val Ser 
                245                 250                 255     


Pro Lys Ala Glu Leu Gly Phe Asp Glu Leu Ser Glu Leu Ile Gly Arg 
            260                 265                 270         


Thr Gln Met Leu Ile Asp Arg Pro Leu Asn Pro Ser Ser Pro Leu Asn 
        275                 280                 285             


Phe Leu Ser Gln Leu Leu Pro Pro Arg Glu Gln Glu Glu Tyr Val Leu 
    290                 295                 300                 


Ala Gln Asn Pro Ser Asp Thr Ala Ala Pro Ile Val Gly Pro Thr Leu 
305                 310                 315                 320 


Arg Arg Leu Leu Asp Glu Thr Ala Asp Phe Ile Glu Ser Pro Asn Ala 
                325                 330                 335     


Ala Glu Val Ile Glu Arg Leu Val His Ser Gly Leu Ser Val Phe Met 
            340                 345                 350         


Asp Lys Leu Ala Val Thr Phe Gly Ala Thr Pro Ala Asp Ser Gly Ser 
        355                 360                 365             


Pro Tyr Pro Val Val Leu Pro Thr Ala Lys Val Lys Leu Pro Ser Ile 
    370                 375                 380                 


Leu Ala Asn Met Ala Arg Gln Ala Gly Gly Met Ala Gln Gly Ser Pro 
385                 390                 395                 400 


Gly Val Glu Asn Glu Tyr Ile Asp Val Met Asn Gln Val Gln Glu Leu 
                405                 410                 415     


Thr Ser Phe Ser Ala Val Val Tyr Ser Ser Phe Asp Trp Ala Leu 
            420                 425                 430     


<210>  4
<211>  395
<212>  PRT
<213>  Yarrowia lipolytica CLIB122 (GenBank Accession No. CAG83356)


<220>
<221>  MISC_FEATURE
<222>  (1)..(395)
<223>  YlPex3Bp

<400>  4

Met Leu Gln Ser Leu Asn Arg Asn Lys Lys Arg Leu Ala Val Ser Thr 
1               5                   10                  15      


Gly Leu Ile Ala Val Ala Tyr Val Val Ile Ser Tyr Thr Thr Lys Arg 
            20                  25                  30          


Leu Ile Glu Lys Gln Glu Gln Lys Leu Glu Glu Glu Arg Ala Lys Glu 
        35                  40                  45              


Arg Leu Lys Gln Leu Phe Ala Gln Thr Gln Asn Glu Ala Ala Phe His 
    50                  55                  60                  


Thr Ala Ser Val Leu Pro Gln Leu Cys Glu Gln Ile Met Glu Phe Val 
65                  70                  75                  80  


Ala Val Glu Lys Ile Ala Glu Gln Leu Gln Asn Met Arg Ala Glu Lys 
                85                  90                  95      


Arg Lys Lys Gln Asn Met Asp Asp Asp Lys His Ser Val Leu Ser Leu 
            100                 105                 110         


Gly Thr Glu Thr Thr Ala Ser Met Ala Asp Gly Gln Lys Met Ser Lys 
        115                 120                 125             


Ile Gln Leu Trp Asp Glu Leu Lys Ile Glu Ser Leu Thr Arg Ile Val 
    130                 135                 140                 


Thr Leu Ile Tyr Cys Val Ser Leu Leu Asn Tyr Leu Ile Arg Leu Gln 
145                 150                 155                 160 


Thr Asn Ile Val Gly Arg Lys Arg Tyr Gln Asn Glu Ala Gly Pro Ala 
                165                 170                 175     


Gly Ala Thr Tyr Asp Met Ser Leu Glu Gln Cys Tyr Thr Trp Leu Leu 
            180                 185                 190         


Thr Arg Gly Trp Lys Ser Val Val Asp Asn Val Arg Arg Ser Val Gln 
        195                 200                 205             


Gln Val Phe Thr Gly Val Asn Pro Arg Gln Asn Leu Ser Leu Asp Glu 
    210                 215                 220                 


Phe Ala Thr Leu Leu Lys Arg Val Gln Thr Leu Val Asn Ser Pro Pro 
225                 230                 235                 240 


Tyr Ser Thr Thr Pro Asn Thr Phe Leu Thr Ser Leu Leu Pro Pro Arg 
                245                 250                 255     


Glu Leu Glu Gln Leu Arg Leu Glu Lys Glu Lys Gln Ser Leu Ser Pro 
            260                 265                 270         


Asn Tyr Thr Tyr Gly Ser Pro Leu Lys Asp Leu Val Phe Glu Ser Ala 
        275                 280                 285             


Gln His Ile Gln Ser Pro Gln Gly Met Ser Ser Phe Arg Ala Ile Ile 
    290                 295                 300                 


Asp Gln Ser Phe Lys Val Phe Leu Glu Lys Val Asn Glu Ser Gln Tyr 
305                 310                 315                 320 


Val Asn Pro Pro Ser Thr Gly Gly Lys Arg Ile Ala Val Gly Ala Leu 
                325                 330                 335     


Gln Pro Pro Ile Ile Ser Gly Gly Pro Lys Lys Val Lys Leu Ala Ser 
            340                 345                 350         


Leu Leu Ser Val Ala Thr Arg Gln Ser Ser Val Ile Ser His Ala Gln 
        355                 360                 365             


Pro Asn Pro Tyr Val Asp Ala Ile Asn Ser Val Ala Glu Tyr Asn Gly 
    370                 375                 380                 


Leu Cys Ala Val Ile Tyr Ser Ser Phe Glu Gln 
385                 390                 395 


<210>  5
<211>  153
<212>  PRT
<213>  Yarrowia lipolytica CLIB122 (GenBank Accession No. CAG79130)


<220>
<221>  MISC_FEATURE
<222>  (1)..(153)
<223>  YlPex4p

<400>  5

Met Ala Ser Gln Lys Arg Leu Ile Lys Glu Leu Ala Ala Tyr Lys Lys 
1               5                   10                  15      


Asp Pro Asn Pro Cys Leu Ala Ser Leu Thr Ala Asp Gly Asp Ser Leu 
            20                  25                  30          


Tyr Lys Trp Thr Ala Val Met Arg Gly Thr Glu Gly Thr Ala Tyr Glu 
        35                  40                  45              


Asn Gly Leu Trp Gln Val Glu Ile Asn Ile Pro Glu Asn Tyr Pro Leu 
    50                  55                  60                  


Gln Pro Pro Thr Met Phe Phe Arg Thr Lys Ile Cys His Pro Asn Ile 
65                  70                  75                  80  


His Phe Glu Thr Gly Glu Val Cys Ile Asp Val Leu Lys Thr Gln Trp 
                85                  90                  95      


Ser Pro Ala Trp Thr Ile Ser Ser Ala Cys Thr Ala Val Ser Ala Met 
            100                 105                 110         


Leu Ser Leu Pro Glu Pro Asp Ser Pro Leu Asn Ile Asp Ala Ala Asn 
        115                 120                 125             


Leu Val Arg Cys Gly Asp Glu Ser Ala Met Glu Gly Leu Val Arg Tyr 
    130                 135                 140                 


Tyr Val Asn Lys Tyr Ala Ser Gly Asn 
145                 150             


<210>  6
<211>  598
<212>  PRT
<213>  Yarrowia lipolytica CLIB122 (GenBank Accession No. CAG78803)


<220>
<221>  MISC_FEATURE
<222>  (1)..(598)
<223>  YlPex5p

<400>  6

Met Ser Phe Met Arg Gly Gly Ser Glu Cys Ser Thr Gly Arg Asn Pro 
1               5                   10                  15      


Leu Ser Gln Phe Thr Lys His Thr Ala Glu Asp Arg Ser Leu Gln His 
            20                  25                  30          


Asp Arg Val Ala Gly Pro Ser Gly Gly Arg Val Gly Gly Met Arg Ser 
        35                  40                  45              


Asn Thr Gly Glu Met Ser Gln Gln Asp Arg Glu Met Met Ala Arg Phe 
    50                  55                  60                  


Gly Ala Ala Gly Pro Glu Gln Ser Ser Phe Asn Tyr Glu Gln Met Arg 
65                  70                  75                  80  


His Glu Leu His Asn Met Gly Ala Gln Gly Gly Gln Ile Pro Gln Val 
                85                  90                  95      


Pro Ser Gln Gln Gly Ala Ala Asn Gly Gly Gln Trp Ala Arg Asp Phe 
            100                 105                 110         


Gly Gly Gln Gln Thr Ala Pro Gly Ala Ala Pro Gln Asp Ala Lys Asn 
        115                 120                 125             


Trp Asn Ala Glu Phe Gln Arg Gly Gly Ser Pro Ala Glu Ala Met Gln 
    130                 135                 140                 


Gln Gln Gly Pro Gly Pro Met Gln Gly Gly Met Gly Met Gly Gly Met 
145                 150                 155                 160 


Pro Met Tyr Gly Met Ala Arg Pro Met Tyr Ser Gly Met Ser Ala Asn 
                165                 170                 175     


Met Ala Pro Gln Phe Gln Pro Gln Gln Ala Asn Ala Arg Val Val Glu 
            180                 185                 190         


Leu Asp Glu Gln Asn Trp Glu Glu Gln Phe Lys Gln Met Asp Ser Ala 
        195                 200                 205             


Val Gly Lys Gly Lys Glu Val Glu Glu Gln Thr Ala Glu Thr Ala Thr 
    210                 215                 220                 


Ala Thr Glu Thr Val Thr Glu Thr Glu Thr Thr Thr Glu Asp Lys Pro 
225                 230                 235                 240 


Met Asp Ile Lys Asn Met Asp Phe Glu Asn Ile Trp Lys Asn Leu Gln 
                245                 250                 255     


Val Asn Val Leu Asp Asn Met Asp Glu Trp Leu Glu Glu Thr Asn Ser 
            260                 265                 270         


Pro Ala Trp Glu Arg Asp Phe His Glu Tyr Thr His Asn Arg Pro Glu 
        275                 280                 285             


Phe Ala Asp Tyr Gln Phe Glu Glu Asn Asn Gln Phe Met Glu His Pro 
    290                 295                 300                 


Asp Pro Phe Lys Ile Gly Val Glu Leu Met Glu Thr Gly Gly Arg Leu 
305                 310                 315                 320 


Ser Glu Ala Ala Leu Ala Phe Glu Ala Ala Val Gln Lys Asn Thr Glu 
                325                 330                 335     


His Ala Glu Ala Trp Gly Arg Leu Gly Ala Cys Gln Ala Gln Asn Glu 
            340                 345                 350         


Lys Glu Asp Pro Ala Ile Arg Ala Leu Glu Arg Cys Ile Lys Leu Glu 
        355                 360                 365             


Pro Gly Asn Leu Ser Ala Leu Met Asn Leu Ser Val Ser Tyr Thr Asn 
    370                 375                 380                 


Glu Gly Tyr Glu Asn Ala Ala Tyr Ala Thr Leu Glu Arg Trp Leu Ala 
385                 390                 395                 400 


Thr Lys Tyr Pro Glu Val Val Asp Gln Ala Arg Asn Gln Glu Pro Arg 
                405                 410                 415     


Leu Gly Asn Glu Asp Lys Phe Gln Leu His Ser Arg Val Thr Glu Leu 
            420                 425                 430         


Phe Ile Arg Ala Ala Gln Leu Ser Pro Asp Gly Ala Asn Ile Asp Ala 
        435                 440                 445             


Asp Val Gln Val Gly Leu Gly Val Leu Phe Tyr Gly Asn Glu Glu Tyr 
    450                 455                 460                 


Asp Lys Ala Ile Asp Cys Phe Asn Ala Ala Ile Ala Val Arg Pro Asp 
465                 470                 475                 480 


Asp Ala Leu Leu Trp Asn Arg Leu Gly Ala Thr Leu Ala Asn Ser His 
                485                 490                 495     


Arg Ser Glu Glu Ala Ile Asp Ala Tyr Tyr Lys Ala Leu Glu Leu Arg 
            500                 505                 510         


Pro Ser Phe Val Arg Ala Arg Tyr Asn Leu Gly Val Ser Cys Ile Asn 
        515                 520                 525             


Ile Gly Cys Tyr Lys Glu Ala Ala Gln Tyr Leu Leu Gly Ala Leu Ser 
    530                 535                 540                 


Met His Lys Val Glu Gly Val Gln Asp Asp Val Leu Ala Asn Gln Ser 
545                 550                 555                 560 


Thr Asn Leu Tyr Asp Thr Leu Lys Arg Val Phe Leu Gly Met Asp Arg 
                565                 570                 575     


Arg Asp Leu Val Ala Lys Val Gly Asn Gly Met Asp Val Asn Gln Phe 
            580                 585                 590         


Arg Asn Glu Phe Glu Phe 
        595             


<210>  7
<211>  1024
<212>  PRT
<213>  Yarrowia lipolytica CLIB122 (GenBank Accession No. CAG82306)


<220>
<221>  MISC_FEATURE
<222>  (1)..(1024)
<223>  YlPex6p

<400>  7

Met Pro Ser Ile Ser His Lys Pro Ile Thr Ala Lys Leu Val Ala Ala 
1               5                   10                  15      


Pro Asp Ala Thr Lys Leu Glu Leu Ser Ser Tyr Leu Tyr Gln Gln Leu 
            20                  25                  30          


Phe Ser Asp Lys Pro Ala Glu Pro Tyr Val Ala Phe Glu Ala Pro Gly 
        35                  40                  45              


Ile Lys Trp Ala Leu Tyr Pro Ala Ser Glu Asp Arg Ser Leu Pro Gln 
    50                  55                  60                  


Tyr Thr Cys Lys Ala Asp Ile Arg His Val Ala Gly Ser Leu Lys Lys 
65                  70                  75                  80  


Phe Met Pro Val Val Leu Lys Arg Val Asn Pro Val Thr Ile Glu His 
                85                  90                  95      


Ala Ile Val Thr Val Pro Ala Ser Gln Tyr Glu Thr Leu Asn Thr Pro 
            100                 105                 110         


Glu Gln Val Leu Lys Ala Leu Glu Pro Gln Leu Asp Lys Asp Arg Pro 
        115                 120                 125             


Val Ile Arg Gln Gly Asp Val Leu Leu Asn Gly Cys Arg Val Arg Leu 
    130                 135                 140                 


Cys Glu Pro Val Asn Gln Gly Lys Val Val Lys Gly Thr Thr Lys Leu 
145                 150                 155                 160 


Thr Val Ala Lys Glu Gln Glu Thr Ile Gln Pro Ala Asp Glu Ala Ala 
                165                 170                 175     


Asp Val Ala Phe Asp Ile Ala Glu Phe Leu Asp Phe Asp Thr Ser Val 
            180                 185                 190         


Ala Lys Thr Arg Glu Ser Thr Asn Leu Gln Val Ala Pro Leu Glu Gly 
        195                 200                 205             


Ala Ile Pro Thr Pro Leu Ser Asp Arg Phe Asp Asp Cys Glu Ser Arg 
    210                 215                 220                 


Gly Phe Val Lys Ser Glu Thr Met Ser Lys Leu Gly Val Phe Ser Gly 
225                 230                 235                 240 


Asp Ile Val Ser Ile Lys Thr Lys Asn Gly Ala Glu Arg Val Leu Arg 
                245                 250                 255     


Leu Phe Ala Tyr Pro Glu Pro Asn Thr Val Lys Tyr Asp Val Val Tyr 
            260                 265                 270         


Val Ser Pro Ile Leu Tyr His Asn Ile Gly Asp Lys Glu Ile Glu Val 
        275                 280                 285             


Thr Pro Asn Gly Glu Thr His Lys Ser Val Gly Glu Ala Leu Asp Ser 
    290                 295                 300                 


Val Leu Glu Ala Ala Glu Glu Val Lys Leu Ala Arg Val Leu Gly Pro 
305                 310                 315                 320 


Thr Thr Thr Asp Arg Thr Phe Gln Thr Ala Tyr His Ala Gly Leu Gln 
                325                 330                 335     


Ala Tyr Phe Lys Pro Val Lys Arg Ala Val Arg Val Gly Asp Leu Ile 
            340                 345                 350         


Pro Ile Pro Phe Asp Ser Ile Leu Ala Arg Thr Ile Gly Glu Asp Pro 
        355                 360                 365             


Glu Met Ser His Ile Pro Leu Glu Ala Leu Ala Val Lys Pro Asp Ser 
    370                 375                 380                 


Val Ala Trp Phe Gln Val Thr Ser Leu Asn Gly Ser Glu Asp Pro Ala 
385                 390                 395                 400 


Ser Lys Gln Tyr Leu Val Asp Ser Ser Gln Thr Lys Leu Ile Glu Gly 
                405                 410                 415     


Gly Thr Thr Ser Ser Ala Val Ile Pro Thr Ser Val Pro Trp Arg Glu 
            420                 425                 430         


Tyr Leu Gly Leu Asp Thr Leu Pro Lys Phe Gly Ser Glu Phe Ala Tyr 
        435                 440                 445             


Ala Asp Lys Ile Arg Asn Leu Val Gln Ile Ser Thr Ser Ala Leu Ser 
    450                 455                 460                 


His Ala Lys Leu Asn Thr Ser Val Leu Leu His Ser Ala Lys Arg Gly 
465                 470                 475                 480 


Val Gly Lys Ser Thr Val Leu Arg Ser Val Ala Ala Gln Cys Gly Ile 
                485                 490                 495     


Ser Val Phe Glu Ile Ser Cys Phe Gly Leu Ile Gly Asp Asn Glu Ala 
            500                 505                 510         


Gln Thr Leu Gly Thr Leu Arg Ala Lys Leu Asp Arg Ala Tyr Gly Cys 
        515                 520                 525             


Ser Pro Cys Val Val Val Leu Gln His Leu Glu Ser Ile Ala Lys Lys 
    530                 535                 540                 


Ser Asp Gln Asp Gly Lys Asp Glu Gly Ile Val Ser Lys Leu Val Asp 
545                 550                 555                 560 


Val Leu Ala Asp Tyr Ser Gly His Gly Val Leu Leu Ala Ala Thr Ser 
                565                 570                 575     


Asn Asp Pro Asp Lys Ile Ser Glu Ala Ile Arg Ser Arg Phe Gln Phe 
            580                 585                 590         


Glu Ile Glu Ile Gly Val Pro Ser Glu Pro Gln Arg Arg Gln Ile Phe 
        595                 600                 605             


Ser His Leu Thr Lys Ser Gly Pro Gly Gly Asp Ser Ile Arg Asn Ala 
    610                 615                 620                 


Pro Ile Ser Leu Arg Ser Asp Val Ser Val Glu Asn Leu Ala Leu Gln 
625                 630                 635                 640 


Ser Ala Gly Leu Thr Pro Pro Asp Leu Thr Ala Ile Val Gln Thr Thr 
                645                 650                 655     


Arg Leu Arg Ala Ile Asp Arg Leu Asn Lys Leu Thr Lys Asp Ser Asp 
            660                 665                 670         


Thr Thr Leu Asp Asp Leu Leu Thr Leu Ser His Gly Thr Leu Gln Leu 
        675                 680                 685             


Thr Pro Ser Asp Phe Asp Asp Ala Ile Ala Asp Ala Arg Gln Lys Tyr 
    690                 695                 700                 


Ser Asp Ser Ile Gly Ala Pro Arg Ile Pro Asn Val Gly Trp Asp Asp 
705                 710                 715                 720 


Val Gly Gly Met Glu Gly Val Lys Lys Asp Ile Leu Asp Thr Ile Glu 
                725                 730                 735     


Thr Pro Leu Lys Tyr Pro His Trp Phe Ser Asp Gly Val Lys Lys Arg 
            740                 745                 750         


Ser Gly Ile Leu Phe Tyr Gly Pro Pro Gly Thr Gly Lys Thr Leu Leu 
        755                 760                 765             


Ala Lys Ala Ile Ala Thr Thr Phe Ser Leu Asn Phe Phe Ser Val Lys 
    770                 775                 780                 


Gly Pro Glu Leu Leu Asn Met Tyr Ile Gly Glu Ser Glu Ala Asn Val 
785                 790                 795                 800 


Arg Arg Val Phe Gln Lys Ala Arg Asp Ala Lys Pro Cys Val Val Phe 
                805                 810                 815     


Phe Asp Glu Leu Asp Ser Val Ala Pro Gln Arg Gly Asn Gln Gly Asp 
            820                 825                 830         


Ser Gly Gly Val Met Asp Arg Ile Val Ser Gln Leu Leu Ala Glu Leu 
        835                 840                 845             


Asp Gly Met Ser Thr Ala Gly Gly Glu Gly Val Phe Val Val Gly Ala 
    850                 855                 860                 


Thr Asn Arg Pro Asp Leu Leu Asp Glu Ala Leu Leu Arg Pro Gly Arg 
865                 870                 875                 880 


Phe Asp Lys Met Leu Tyr Leu Gly Ile Ser Asp Thr His Glu Lys Gln 
                885                 890                 895     


Gln Thr Ile Met Glu Ala Leu Thr Arg Lys Phe Arg Leu Ala Ala Asp 
            900                 905                 910         


Val Ser Leu Glu Ala Ile Ser Lys Arg Cys Pro Phe Thr Phe Thr Gly 
        915                 920                 925             


Ala Asp Phe Tyr Ala Leu Cys Ser Asp Ala Met Leu Asn Ala Met Thr 
    930                 935                 940                 


Arg Thr Ala Asn Glu Val Asp Ala Lys Ile Lys Leu Leu Asn Lys Asn 
945                 950                 955                 960 


Arg Glu Glu Ala Gly Glu Glu Pro Val Ser Ile Arg Trp Trp Phe Asp 
                965                 970                 975     


His Glu Ala Thr Lys Ser Asp Ile Glu Val Glu Val Ala Gln Gln Asp 
            980                 985                 990         


Phe Glu Lys Ala Lys Asp Glu Leu  Ser Pro Ser Val Ser  Ala Glu Glu 
        995                 1000                 1005             


Leu Gln  His Tyr Leu Lys Leu  Arg Gln Gln Phe Glu  Gly Gly Lys 
    1010                 1015                 1020             


Lys 
    


<210>  8
<211>  356
<212>  PRT
<213>  Yarrowia lipolytica CLIB122 (GenBank Accession No. CAG78389)


<220>
<221>  MISC_FEATURE
<222>  (1)..(356)
<223>  YlPex7p

<400>  8

Met Leu Gly Phe Lys Thr Gln Gly Phe Asn Gly Tyr Ala Ala Asn Tyr 
1               5                   10                  15      


Ser Pro Phe Phe Asn Asp Lys Ile Ala Val Gly Thr Ala Ala Asn Tyr 
            20                  25                  30          


Gly Leu Val Gly Asn Gly Lys Leu Phe Ile Leu Gly Ile Ser Pro Glu 
        35                  40                  45              


Gly Arg Met Val Cys Glu Gly Gln Phe Asp Thr Gln Asp Gly Ile Phe 
    50                  55                  60                  


Asp Val Ala Trp Ser Glu Gln His Glu Asn His Val Ala Thr Ala Cys 
65                  70                  75                  80  


Gly Asp Gly Ser Val Lys Leu Phe Asp Ile Lys Ala Gly Ala Phe Pro 
                85                  90                  95      


Leu Val Ser Phe Lys Glu His Thr Arg Glu Val Phe Ser Val Asn Trp 
            100                 105                 110         


Asn Met Ala Asn Lys Ala Leu Phe Cys Thr Ser Ser Trp Asp Ser Thr 
        115                 120                 125             


Ile Lys Ile Trp Thr Pro Glu Arg Thr Asn Ser Ile Met Thr Leu Gly 
    130                 135                 140                 


Gln Pro Ala Pro Ala Gln Gly Thr Asn Ala Ser Ala His Ile Gly Arg 
145                 150                 155                 160 


Gln Thr Ala Pro Asn Gln Ala Ala Ala Gln Glu Cys Ile Tyr Ser Ala 
                165                 170                 175     


Lys Phe Ser Pro His Thr Asp Ser Ile Ile Ala Ser Ala His Ser Thr 
            180                 185                 190         


Gly Met Val Lys Val Trp Asp Thr Arg Ala Pro Gln Pro Leu Gln Gln 
        195                 200                 205             


Gln Phe Ser Thr Gln Gln Thr Glu Ser Gly Gly Pro Pro Glu Val Leu 
    210                 215                 220                 


Ser Leu Asp Trp Asn Lys Tyr Arg Pro Thr Val Ile Ala Thr Gly Gly 
225                 230                 235                 240 


Val Asp Arg Ser Val Gln Val Tyr Asp Ile Arg Met Thr Gln Pro Ala 
                245                 250                 255     


Ala Asn Gln Pro Val Gln Pro Leu Ser Leu Ile Leu Gly His Arg Leu 
            260                 265                 270         


Pro Val Arg Gly Val Ser Trp Ser Pro His His Ala Asp Leu Leu Leu 
        275                 280                 285             


Ser Cys Ser Tyr Asp Met Thr Ala Arg Val Trp Arg Asp Ala Ser Thr 
    290                 295                 300                 


Gly Gly Asn Tyr Leu Ala Arg Gln Arg Gly Gly Thr Glu Val Lys Cys 
305                 310                 315                 320 


Met Asp Arg His Thr Glu Phe Val Ile Gly Gly Asp Trp Ser Leu Trp 
                325                 330                 335     


Gly Asp Pro Gly Trp Ile Thr Thr Val Gly Trp Asp Gln Met Val Tyr 
            340                 345                 350         


Val Trp His Ala 
        355     


<210>  9
<211>  671
<212>  PRT
<213>  Yarrowia lipolytica CLIB122 (GenBank Accession No. CAG80447)


<220>
<221>  MISC_FEATURE
<222>  (1)..(671)
<223>  YlPex8p

<400>  9

Met Asn Lys Tyr Leu Val Pro Pro Pro Gln Ala Asn Arg Thr Val Thr 
1               5                   10                  15      


Asn Leu Asp Leu Leu Ile Asn Asn Leu Arg Gly Ser Ser Thr Pro Gly 
            20                  25                  30          


Ala Ala Glu Val Asp Thr Arg Asp Ile Leu Gln Arg Ile Val Phe Ile 
        35                  40                  45              


Leu Pro Thr Ile Lys Asn Pro Leu Asn Leu Asp Leu Val Ile Lys Glu 
    50                  55                  60                  


Ile Ile Asn Ser Pro Arg Leu Leu Pro Pro Leu Ile Asp Leu His Asp 
65                  70                  75                  80  


Tyr Gln Gln Leu Thr Asp Ala Phe Arg Ala Thr Ile Lys Arg Lys Ala 
                85                  90                  95      


Leu Val Thr Asp Pro Thr Ile Ser Phe Glu Ala Trp Leu Glu Thr Cys 
            100                 105                 110         


Phe Gln Val Ile Thr Arg Phe Ala Gly Pro Gly Trp Lys Lys Leu Pro 
        115                 120                 125             


Leu Leu Ala Gly Leu Ile Leu Ala Asp Tyr Asp Ile Ser Ala Asp Gly 
    130                 135                 140                 


Pro Thr Leu Glu Arg Lys Pro Gly Phe Pro Ser Lys Leu Lys His Leu 
145                 150                 155                 160 


Leu Lys Arg Glu Phe Val Thr Thr Phe Asp Gln Cys Leu Ser Ile Asp 
                165                 170                 175     


Thr Arg Asn Arg Ser Asp Ala Thr Lys Trp Val Pro Val Leu Ala Cys 
            180                 185                 190         


Ile Ser Ile Ala Gln Val Tyr Ser Leu Leu Gly Asp Val Ala Ile Asn 
        195                 200                 205             


Tyr Arg Arg Phe Leu Gln Val Gly Leu Asp Leu Ile Phe Ser Asn Tyr 
    210                 215                 220                 


Gly Leu Glu Met Gly Thr Ala Leu Ala Arg Leu His Ala Glu Ser Gly 
225                 230                 235                 240 


Gly Asp Ala Thr Thr Ala Gly Gly Leu Ile Gly Lys Lys Leu Lys Glu 
                245                 250                 255     


Pro Val Val Ala Leu Leu Asn Thr Phe Ala His Ile Ala Ser Ser Cys 
            260                 265                 270         


Ile Val His Val Asp Ile Asp Tyr Ile Asp Arg Ile Gln Asn Lys Ile 
        275                 280                 285             


Ile Leu Val Cys Glu Asn Gln Ala Glu Thr Trp Arg Ile Leu Thr Ile 
    290                 295                 300                 


Glu Ser Pro Thr Val Met His His Gln Glu Ser Val Gln Tyr Leu Lys 
305                 310                 315                 320 


Trp Glu Leu Phe Thr Leu Cys Ile Ile Met Gln Gly Ile Ala Asn Met 
                325                 330                 335     


Leu Leu Thr Gln Lys Met Asn Gln Phe Met Tyr Leu Gln Leu Ala Tyr 
            340                 345                 350         


Lys Gln Leu Gln Ala Leu His Ser Ile Tyr Phe Ile Val Asp Gln Met 
        355                 360                 365             


Gly Ser Gln Phe Ala Ala Tyr Asp Tyr Val Phe Phe Ser Ala Ile Asp 
    370                 375                 380                 


Val Leu Leu Ser Glu Tyr Ala Pro Tyr Ile Lys Asn Arg Gly Thr Ile 
385                 390                 395                 400 


Pro Pro Asn Lys Glu Phe Val Ala Glu Arg Leu Ala Ala Asn Leu Ala 
                405                 410                 415     


Gly Thr Ser Asn Val Gly Ser His Leu Pro Ile Asp Arg Ser Arg Val 
            420                 425                 430         


Leu Phe Ala Leu Asn Tyr Tyr Glu Gln Leu Val Thr Val Cys His Asp 
        435                 440                 445             


Ser Cys Val Glu Thr Ile Ile Tyr Pro Met Ala Arg Ser Phe Leu Tyr 
    450                 455                 460                 


Pro Thr Ser Asp Ile Gln Gln Leu Lys Pro Leu Val Glu Ala Ala His 
465                 470                 475                 480 


Ser Val Ile Leu Ala Gly Leu Ala Val Pro Thr Asn Ala Val Val Asn 
                485                 490                 495     


Ala Lys Leu Ile Pro Glu Tyr Met Gly Gly Val Leu Pro Leu Phe Pro 
            500                 505                 510         


Gly Val Phe Ser Trp Asn Gln Phe Val Leu Ala Ile Gln Ser Ile Val 
        515                 520                 525             


Asn Thr Val Ser Pro Pro Ser Glu Val Phe Lys Thr Asn Gln Lys Leu 
    530                 535                 540                 


Phe Arg Leu Val Leu Asp Ser Leu Met Lys Lys Cys Arg Asp Thr Pro 
545                 550                 555                 560 


Val Gly Ile Pro Val Pro His Ser Val Thr Val Ser Gln Glu Gln Glu 
                565                 570                 575     


Asp Ile Pro Pro Thr Gln Arg Ala Val Val Met Leu Ala Leu Ile Asn 
            580                 585                 590         


Ser Leu Pro Tyr Val Asp Ile Arg Ser Phe Glu Leu Trp Leu Gln Glu 
        595                 600                 605             


Thr Trp Asn Met Ile Glu Ala Thr Pro Met Leu Ala Glu Asn Ala Pro 
    610                 615                 620                 


Asn Lys Glu Leu Ala His Ala Glu His Glu Phe Leu Val Leu Glu Met 
625                 630                 635                 640 


Trp Lys Met Ile Ser Gly Asn Ile Asp Gln Arg Leu Asn Asp Val Ala 
                645                 650                 655     


Ile Arg Trp Trp Tyr Lys Lys Asn Ala Arg Val His Gly Thr Leu 
            660                 665                 670     


<210>  10
<211>  377
<212>  PRT
<213>  Yarrowia lipolytica CLIB122 (GenBank Accession No. CAG81606)


<220>
<221>  MISC_FEATURE
<222>  (1)..(377)
<223>  YlPex10p

<400>  10

Met Trp Gly Ser Ser His Ala Phe Ala Gly Glu Ser Asp Leu Thr Leu 
1               5                   10                  15      


Gln Leu His Thr Arg Ser Asn Met Ser Asp Asn Thr Thr Ile Lys Lys 
            20                  25                  30          


Pro Ile Arg Pro Lys Pro Ile Arg Thr Glu Arg Leu Pro Tyr Ala Gly 
        35                  40                  45              


Ala Ala Glu Ile Ile Arg Ala Asn Gln Lys Asp His Tyr Phe Glu Ser 
    50                  55                  60                  


Val Leu Glu Gln His Leu Val Thr Phe Leu Gln Lys Trp Lys Gly Val 
65                  70                  75                  80  


Arg Phe Ile His Gln Tyr Lys Glu Glu Leu Glu Thr Ala Ser Lys Phe 
                85                  90                  95      


Ala Tyr Leu Gly Leu Cys Thr Leu Val Gly Ser Lys Thr Leu Gly Glu 
            100                 105                 110         


Glu Tyr Thr Asn Leu Met Tyr Thr Ile Arg Asp Arg Thr Ala Leu Pro 
        115                 120                 125             


Gly Val Val Arg Arg Phe Gly Tyr Val Leu Ser Asn Thr Leu Phe Pro 
    130                 135                 140                 


Tyr Leu Phe Val Arg Tyr Met Gly Lys Leu Arg Ala Lys Leu Met Arg 
145                 150                 155                 160 


Glu Tyr Pro His Leu Val Glu Tyr Asp Glu Asp Glu Pro Val Pro Ser 
                165                 170                 175     


Pro Glu Thr Trp Lys Glu Arg Val Ile Lys Thr Phe Val Asn Lys Phe 
            180                 185                 190         


Asp Lys Phe Thr Ala Leu Glu Gly Phe Thr Ala Ile His Leu Ala Ile 
        195                 200                 205             


Phe Tyr Val Tyr Gly Ser Tyr Tyr Gln Leu Ser Lys Arg Ile Trp Gly 
    210                 215                 220                 


Met Arg Tyr Val Phe Gly His Arg Leu Asp Lys Asn Glu Pro Arg Ile 
225                 230                 235                 240 


Gly Tyr Glu Met Leu Gly Leu Leu Ile Phe Ala Arg Phe Ala Thr Ser 
                245                 250                 255     


Phe Val Gln Thr Gly Arg Glu Tyr Leu Gly Ala Leu Leu Glu Lys Ser 
            260                 265                 270         


Val Glu Lys Glu Ala Gly Glu Lys Glu Asp Glu Lys Glu Ala Val Val 
        275                 280                 285             


Pro Lys Lys Lys Ser Ser Ile Pro Phe Ile Glu Asp Thr Glu Gly Glu 
    290                 295                 300                 


Thr Glu Asp Lys Ile Asp Leu Glu Asp Pro Arg Gln Leu Lys Phe Ile 
305                 310                 315                 320 


Pro Glu Ala Ser Arg Ala Cys Thr Leu Cys Leu Ser Tyr Ile Ser Ala 
                325                 330                 335     


Pro Ala Cys Thr Pro Cys Gly His Phe Phe Cys Trp Asp Cys Ile Ser 
            340                 345                 350         


Glu Trp Val Arg Glu Lys Pro Glu Cys Pro Leu Cys Arg Gln Gly Val 
        355                 360                 365             


Arg Glu Gln Asn Leu Leu Pro Ile Arg 
    370                 375         


<210>  11
<211>  408
<212>  PRT
<213>  Yarrowia lipolytica CLIB122 (GenBank Accession No. CAG81532)


<220>
<221>  MISC_FEATURE
<222>  (1)..(408)
<223>  YlPex12p

<400>  11

Met Asp Tyr Phe Ser Ser Leu Asn Ala Ser Gln Leu Asp Pro Asp Val 
1               5                   10                  15      


Pro Thr Leu Phe Glu Leu Leu Ser Ala Lys Gln Leu Glu Gly Leu Ile 
            20                  25                  30          


Ala Pro Ser Val Arg Tyr Ile Leu Ala Phe Tyr Ala Gln Arg His Pro 
        35                  40                  45              


Arg Tyr Leu Leu Arg Ile Val Asn Arg Tyr Asp Glu Leu Tyr Ala Leu 
    50                  55                  60                  


Phe Met Gly Leu Val Glu Tyr Tyr Asn Leu Lys Thr Trp Asn Ala Ser 
65                  70                  75                  80  


Phe Thr Glu Lys Phe Tyr Gly Leu Lys Arg Thr Gln Ile Leu Thr Asn 
                85                  90                  95      


Pro Ala Leu Arg Thr Arg Gln Ala Val Pro Asp Leu Val Glu Ala Glu 
            100                 105                 110         


Lys Arg Leu Ser Lys Lys Lys Ile Trp Gly Ser Leu Phe Phe Leu Ile 
        115                 120                 125             


Val Val Pro Tyr Val Lys Glu Lys Leu Asp Ala Arg Tyr Glu Arg Leu 
    130                 135                 140                 


Lys Gly Arg Tyr Leu Ala Arg Asp Ile Asn Glu Glu Arg Ile Glu Ile 
145                 150                 155                 160 


Lys Arg Thr Gly Thr Ala Gln Gln Ile Ala Val Phe Glu Phe Asp Tyr 
                165                 170                 175     


Trp Leu Leu Lys Leu Tyr Pro Ile Val Thr Met Gly Cys Thr Thr Ala 
            180                 185                 190         


Thr Leu Ala Phe His Met Leu Phe Leu Phe Ser Val Thr Arg Ala Tyr 
        195                 200                 205             


Ser Ile Asp Asp Phe Leu Leu Asn Ile Gln Phe Ser Arg Met Thr Arg 
    210                 215                 220                 


Tyr Asp Tyr Gln Met Glu Thr Gln Arg Asp Ser Arg Asn Ala Ala Asn 
225                 230                 235                 240 


Val Ala His Thr Met Lys Ser Ile Ser Glu Tyr Pro Val Ala Glu Arg 
                245                 250                 255     


Val Met Leu Leu Leu Thr Thr Lys Ala Gly Ala Asn Ala Met Arg Ser 
            260                 265                 270         


Ala Ala Leu Ser Gly Leu Ser Tyr Val Leu Pro Thr Ser Ile Phe Ala 
        275                 280                 285             


Leu Lys Phe Leu Glu Trp Trp Tyr Ala Ser Asp Phe Ala Arg Gln Leu 
    290                 295                 300                 


Asn Gln Lys Arg Arg Gly Asp Leu Glu Asp Asn Leu Pro Val Pro Asp 
305                 310                 315                 320 


Lys Val Lys Gly Ala Asp Lys Leu Ala Glu Ser Val Ala Lys Trp Lys 
                325                 330                 335     


Glu Asp Thr Ser Lys Cys Pro Leu Cys Ser Lys Glu Leu Val Asn Pro 
            340                 345                 350         


Thr Val Ile Glu Ser Gly Tyr Val Phe Cys Tyr Thr Cys Ile Tyr Arg 
        355                 360                 365             


His Leu Glu Asp Gly Asp Glu Glu Thr Gly Gly Arg Cys Pro Val Thr 
    370                 375                 380                 


Gly Gln Lys Leu Leu Gly Cys Arg Trp Gln Asp Asp Val Trp Gln Val 
385                 390                 395                 400 


Thr Gly Leu Arg Arg Leu Met Val 
                405             


<210>  12
<211>  412
<212>  PRT
<213>  Yarrowia lipolytica CLIB122 (GenBank Accession No. CAG81789)


<220>
<221>  MISC_FEATURE
<222>  (1)..(412)
<223>  YlPex13p

<400>  12

Met Ser Val Pro Arg Pro Lys Pro Trp Glu Gly Ala Ser Gly Ser Ser 
1               5                   10                  15      


Ala Ala Thr Ala Thr Pro Ala Ala Thr Ala Thr Pro Ala Ser Thr Asp 
            20                  25                  30          


Ala Val Ser Ser Ser Ala Gly Ser Ala Thr Gly Ala Pro Glu Leu Pro 
        35                  40                  45              


Ser Arg Pro Ser Ala Met Gly Ser Thr Ser Asn Ala Leu Ser Ser Pro 
    50                  55                  60                  


Met Gly Ser Ser Met Asn Ser Gly Tyr Gly Gly Met Asn Ser Gly Tyr 
65                  70                  75                  80  


Gly Gly Met Gly Ser Ser Tyr Gly Ser Gly Tyr Gly Ser Ser Tyr Gly 
                85                  90                  95      


Met Gly Ser Ser Tyr Gly Ser Gly Tyr Gly Ser Gly Leu Gly Gly Tyr 
            100                 105                 110         


Gly Ser Tyr Gly Gly Met Gly Gly Met Gly Gly Met Tyr Gly Ser Arg 
        115                 120                 125             


Tyr Gly Gly Tyr Gly Ser Tyr Gly Gly Met Gly Gly Tyr Gly Gly Tyr 
    130                 135                 140                 


Gly Gly Met Gly Gly Gly Pro Met Gly Gln Asn Gly Leu Ala Gly Gly 
145                 150                 155                 160 


Thr Gln Ala Thr Phe Gln Leu Ile Glu Ser Ile Val Gly Ala Val Gly 
                165                 170                 175     


Gly Phe Ala Gln Met Leu Glu Ser Thr Tyr Met Ala Thr Gln Ser Ser 
            180                 185                 190         


Phe Phe Ala Met Val Ser Val Ala Glu Gln Phe Gly Asn Leu Lys Asn 
        195                 200                 205             


Thr Leu Gly Ser Leu Leu Gly Ile Tyr Ala Ile Met Arg Trp Ala Arg 
    210                 215                 220                 


Arg Leu Val Ala Lys Leu Ser Gly Gln Pro Val Thr Gly Ala Asn Gly 
225                 230                 235                 240 


Ile Thr Pro Ala Gly Phe Ala Lys Phe Glu Ala Thr Gly Gly Ala Ala 
                245                 250                 255     


Gly Pro Gly Arg Gly Pro Arg Pro Ser Tyr Lys Pro Leu Leu Phe Phe 
            260                 265                 270         


Leu Thr Ala Val Phe Gly Leu Pro Tyr Leu Leu Gly Arg Leu Ile Lys 
        275                 280                 285             


Ala Leu Ala Ala Lys Gln Glu Gly Met Tyr Asp Glu His Gly Asn Leu 
    290                 295                 300                 


Leu Pro Gly Ala Gln Met Gly Met Gly Gly Pro Gly Met Glu Gly Gly 
305                 310                 315                 320 


Ala Glu Ile Asp Pro Ser Lys Leu Glu Phe Cys Arg Ala Asn Phe Asp 
                325                 330                 335     


Phe Val Pro Glu Asn Pro Gln Leu Glu Leu Glu Leu Arg Lys Gly Asp 
            340                 345                 350         


Leu Val Ala Val Leu Ala Lys Thr Asp Pro Met Gly Asn Pro Ser Gln 
        355                 360                 365             


Trp Trp Arg Val Arg Thr Arg Asp Gly Arg Ser Gly Tyr Val Pro Ala 
    370                 375                 380                 


Asn Tyr Leu Glu Val Ile Pro Arg Pro Ala Val Glu Ala Pro Lys Lys 
385                 390                 395                 400 


Val Glu Glu Ile Gly Ala Ser Ala Val Pro Val Asn 
                405                 410         


<210>  13
<211>  380
<212>  PRT
<213>  Yarrowia lipolytica CLIB122 (GenBank Accession No. CAG79323)


<220>
<221>  MISC_FEATURE
<222>  (1)..(380)
<223>  YlPex14p

<400>  13

Met Ile Pro Ser Cys Leu Ser Thr Gln His Met Ala Pro Arg Glu Asp 
1               5                   10                  15      


Leu Val Gln Ser Ala Val Ala Phe Leu Asn Asp Pro Gln Ala Ala Thr 
            20                  25                  30          


Ala Pro Leu Ala Lys Arg Ile Glu Phe Leu Glu Ser Lys Asp Met Thr 
        35                  40                  45              


Pro Glu Glu Ile Glu Glu Ala Leu Lys Arg Ala Gly Ser Gly Ser Ala 
    50                  55                  60                  


Gln Ser His Pro Gly Ser Val Val Ser His Gly Gly Ala Ala Pro Thr 
65                  70                  75                  80  


Val Pro Ala Ser Tyr Ala Phe Gln Ser Ala Pro Pro Leu Pro Glu Arg 
                85                  90                  95      


Asp Trp Lys Asp Val Phe Ile Met Ala Thr Val Thr Val Gly Val Gly 
            100                 105                 110         


Phe Gly Leu Tyr Thr Val Ala Lys Arg Tyr Leu Met Pro Leu Ile Leu 
        115                 120                 125             


Pro Pro Thr Pro Pro Ser Leu Glu Ala Asp Lys Glu Ala Leu Glu Ala 
    130                 135                 140                 


Glu Phe Ala Arg Val Gln Gly Leu Leu Asp Gln Val Gln Gln Asp Thr 
145                 150                 155                 160 


Glu Glu Val Lys Asn Ser Gln Val Glu Val Ala Lys Arg Val Thr Asp 
                165                 170                 175     


Ala Leu Lys Gly Val Glu Glu Thr Ile Asp Gln Leu Lys Ser Gln Thr 
            180                 185                 190         


Lys Lys Arg Asp Asp Glu Met Lys Leu Val Thr Ala Glu Val Glu Arg 
        195                 200                 205             


Ile Arg Asp Arg Leu Pro Lys Asn Ile Asp Lys Leu Lys Asp Ser Gln 
    210                 215                 220                 


Glu Gln Gly Leu Ala Asp Ile Gln Ser Glu Leu Lys Ser Leu Lys Gln 
225                 230                 235                 240 


Leu Leu Ser Thr Arg Thr Ala Ala Ser Ser Gly Pro Lys Leu Pro Pro 
                245                 250                 255     


Ile Pro Pro Pro Ser Ser Tyr Leu Thr Arg Lys Ala Ser Pro Ala Val 
            260                 265                 270         


Pro Ala Ala Ala Pro Ala Pro Val Thr Pro Gly Ser Pro Val His Asn 
        275                 280                 285             


Val Ser Ser Ser Ser Thr Val Pro Ala Asp Arg Asp Asp Phe Ile Pro 
    290                 295                 300                 


Thr Pro Ala Gly Ala Val Pro Met Ile Pro Gln Pro Ala Ser Met Ser 
305                 310                 315                 320 


Ser Ser Ser Thr Ser Thr Val Pro Asn Ser Ala Ile Ser Ser Ala Pro 
                325                 330                 335     


Ser Pro Ile Gln Glu Pro Glu Pro Phe Val Pro Glu Pro Gly Asn Ser 
            340                 345                 350         


Ala Val Lys Lys Pro Ala Pro Lys Ala Ser Ile Pro Ala Trp Gln Leu 
        355                 360                 365             


Ala Ala Leu Glu Lys Glu Lys Glu Lys Glu Lys Glu 
    370                 375                 380 


<210>  14
<211>  391
<212>  PRT
<213>  Yarrowia lipolytica CLIB122 (GenBank Accession No. CAG79622)


<220>
<221>  MISC_FEATURE
<222>  (1)..(391)
<223>  YlPex16p

<400>  14

Met Thr Asp Lys Leu Val Lys Val Met Gln Lys Lys Lys Ser Ala Pro 
1               5                   10                  15      


Gln Thr Trp Leu Asp Ser Tyr Asp Lys Phe Leu Val Arg Asn Ala Ala 
            20                  25                  30          


Ser Ile Gly Ser Ile Glu Ser Thr Leu Arg Thr Val Ser Tyr Val Leu 
        35                  40                  45              


Pro Gly Arg Phe Asn Asp Val Glu Ile Ala Thr Glu Thr Leu Tyr Ala 
    50                  55                  60                  


Val Leu Asn Val Leu Gly Leu Tyr His Asp Thr Ile Ile Ala Arg Ala 
65                  70                  75                  80  


Val Ala Ala Ser Pro Asn Ala Ala Ala Val Tyr Arg Pro Ser Pro His 
                85                  90                  95      


Asn Arg Tyr Thr Asp Trp Phe Ile Lys Asn Arg Lys Gly Tyr Lys Tyr 
            100                 105                 110         


Ala Ser Arg Ala Val Thr Phe Val Lys Phe Gly Glu Leu Val Ala Glu 
        115                 120                 125             


Met Val Ala Lys Lys Asn Gly Gly Glu Met Ala Arg Trp Lys Cys Ile 
    130                 135                 140                 


Ile Gly Ile Glu Gly Ile Lys Ala Gly Leu Arg Ile Tyr Met Leu Gly 
145                 150                 155                 160 


Ser Thr Leu Tyr Gln Pro Leu Cys Thr Thr Pro Tyr Pro Asp Arg Glu 
                165                 170                 175     


Val Thr Gly Glu Leu Leu Glu Thr Ile Cys Arg Asp Glu Gly Glu Leu 
            180                 185                 190         


Asp Ile Glu Lys Gly Leu Met Asp Pro Gln Trp Lys Met Pro Arg Thr 
        195                 200                 205             


Gly Arg Thr Ile Pro Glu Ile Ala Pro Thr Asn Val Glu Gly Tyr Leu 
    210                 215                 220                 


Leu Thr Lys Val Leu Arg Ser Glu Asp Val Asp Arg Pro Tyr Asn Leu 
225                 230                 235                 240 


Leu Ser Arg Leu Asp Asn Trp Gly Val Val Ala Glu Leu Leu Ser Ile 
                245                 250                 255     


Leu Arg Pro Leu Ile Tyr Ala Cys Leu Leu Phe Arg Gln His Val Asn 
            260                 265                 270         


Lys Thr Val Pro Ala Ser Thr Lys Ser Lys Phe Pro Phe Leu Asn Ser 
        275                 280                 285             


Pro Trp Ala Pro Trp Ile Ile Gly Leu Val Ile Glu Ala Leu Ser Arg 
    290                 295                 300                 


Lys Met Met Gly Ser Trp Leu Leu Arg Gln Arg Gln Ser Gly Lys Thr 
305                 310                 315                 320 


Pro Thr Ala Leu Asp Gln Met Glu Val Lys Gly Arg Thr Asn Leu Leu 
                325                 330                 335     


Gly Trp Trp Leu Phe Arg Gly Glu Phe Tyr Gln Ala Tyr Thr Arg Pro 
            340                 345                 350         


Leu Leu Tyr Ser Ile Val Ala Arg Leu Glu Lys Ile Pro Gly Leu Gly 
        355                 360                 365             


Leu Phe Gly Ala Leu Ile Ser Asp Tyr Leu Tyr Leu Phe Asp Arg Tyr 
    370                 375                 380                 


Tyr Phe Thr Ala Ser Thr Leu 
385                 390     


<210>  15
<211>  225
<212>  PRT
<213>  Yarrowia lipolytica CLIB122 (GenBank Accession No. CAG84025)


<220>
<221>  MISC_FEATURE
<222>  (1)..(225)
<223>  YlPex17p

<400>  15

Met Ser Ala Phe Pro Glu Pro Ser Ser Phe Glu Ile Glu Phe Ala Lys 
1               5                   10                  15      


Gln Met Asn Arg Pro Arg Thr Val Gln Phe Lys Gln Leu Val Ala Val 
            20                  25                  30          


Leu Tyr Ile Phe Gly Gly Thr Ser Ala Leu Ile Tyr Ile Ile Ser Lys 
        35                  40                  45              


Thr Ile Leu Asn Pro Leu Phe Glu Glu Leu Thr Phe Ala Arg Ser Glu 
    50                  55                  60                  


Tyr Ala Ile His Ala Arg Arg Leu Met Glu Gln Leu Asn Ala Lys Leu 
65                  70                  75                  80  


Ser Ser Met Ala Ser Tyr Ile Pro Pro Val Arg Ala Leu Gln Gly Gln 
                85                  90                  95      


Arg Phe Val Asp Ala Gln Thr Gln Thr Glu Asp Glu Glu Gly Glu Asp 
            100                 105                 110         


Ile Pro Asn Pro Ser Leu Gly Lys Ser Ser His Val Ser Phe Gly Glu 
        115                 120                 125             


Ser Pro Met Gln Leu Lys Leu Ala Glu Lys Glu Lys Gln Gln Lys Leu 
    130                 135                 140                 


Ile Asp Asp Ser Val Asp Asn Leu Glu Arg Leu Ala Asp Ser Leu Lys 
145                 150                 155                 160 


His Ala Gly Glu Val Ser Asp Leu Ser Ala Leu Ser Gly Phe Lys Tyr 
                165                 170                 175     


Gln Val Glu Glu Leu Thr Asn Tyr Ser Asp Gln Leu Ala Met Ser Gly 
            180                 185                 190         


Tyr Ser Met Met Lys Ser Gly Leu Pro Gly His Glu Thr Ala Met Ser 
        195                 200                 205             


Glu Thr Lys Lys Glu Ile Arg Ser Leu Lys Gly Ser Val Leu Ser Val 
    210                 215                 220                 


Arg 
225 


<210>  16
<211>  324
<212>  PRT
<213>  Yarrowia lipolytica (GenBank Accession No. AAK84827)


<220>
<221>  MISC_FEATURE
<222>  (1)..(324)
<223>  YlPex19p

<400>  16

Met Ser His Glu Glu Asp Leu Asp Asp Leu Asp Asp Phe Leu Asp Glu 
1               5                   10                  15      


Phe Asp Glu Gln Val Leu Ser Lys Pro Pro Gly Ala Gln Lys Asp Ala 
            20                  25                  30          


Thr Pro Thr Thr Ser Thr Ala Pro Thr Thr Ala Glu Ala Lys Pro Asp 
        35                  40                  45              


Ala Thr Lys Lys Ser Thr Glu Thr Ser Gly Thr Asp Ser Lys Thr Glu 
    50                  55                  60                  


Gly Ala Asp Thr Ala Asp Lys Asn Ala Ala Thr Asp Ser Ala Glu Ala 
65                  70                  75                  80  


Gly Ala Glu Lys Val Ser Leu Pro Asn Leu Glu Asp Gln Leu Ala Gly 
                85                  90                  95      


Leu Lys Met Asp Asp Phe Leu Lys Asp Ile Glu Ala Asp Pro Glu Ser 
            100                 105                 110         


Lys Ala Gln Phe Glu Ser Leu Leu Lys Glu Ile Asn Asn Val Thr Ser 
        115                 120                 125             


Ala Thr Ala Ser Glu Lys Ala Gln Gln Pro Lys Ser Phe Lys Glu Thr 
    130                 135                 140                 


Ile Ser Ala Thr Ala Asp Arg Leu Asn Gln Ser Asn Gln Glu Met Gly 
145                 150                 155                 160 


Asp Met Pro Leu Gly Asp Asp Met Leu Ala Gly Leu Met Glu Gln Leu 
                165                 170                 175     


Ser Gly Ala Gly Gly Phe Gly Glu Gly Gly Glu Gly Asp Phe Gly Asp 
            180                 185                 190         


Met Leu Gly Gly Ile Met Arg Gln Leu Ala Ser Lys Glu Val Leu Tyr 
        195                 200                 205             


Gln Pro Leu Lys Glu Met His Asp Asn Tyr Pro Lys Trp Trp Asp Glu 
    210                 215                 220                 


His Gly Ser Lys Val Thr Glu Glu Lys Glu Arg Asp Arg Leu Lys Leu 
225                 230                 235                 240 


Gln Gln Asp Ile Val Gly Lys Ile Cys Ala Lys Phe Glu Asp Pro Ser 
                245                 250                 255     


Tyr Ser Asp Asp Ser Glu Ala Asp Arg Ala Val Ile Thr Gln Leu Met 
            260                 265                 270         


Asp Glu Met Gln Glu Thr Gly Ala Pro Pro Asp Glu Ile Met Ser Asn 
        275                 280                 285             


Val Ala Asp Gly Ser Ile Pro Gly Gly Leu Asp Gly Leu Gly Leu Gly 
    290                 295                 300                 


Gly Leu Gly Gly Gly Lys Met Pro Glu Met Pro Glu Asn Met Pro Glu 
305                 310                 315                 320 


Cys Asn Gln Gln 
                


<210>  17
<211>  417
<212>  PRT
<213>  Yarrowia lipolytica CLIB122 (GenBank Accession No. CAG79226)


<220>
<221>  MISC_FEATURE
<222>  (1)..(417)
<223>  YlPex20p

<400>  17

Met Ala Ser Cys Gly Pro Ser Asn Ala Leu Gln Asn Leu Ser Lys His 
1               5                   10                  15      


Ala Ser Ala Asp Arg Ser Leu Gln His Asp Arg Met Ala Pro Gly Gly 
            20                  25                  30          


Ala Pro Gly Ala Gln Arg Gln Gln Phe Arg Ser Gln Thr Gln Gly Gly 
        35                  40                  45              


Gln Leu Asn Asn Glu Phe Gln Gln Phe Ala Gln Ala Gly Pro Ala His 
    50                  55                  60                  


Asn Ser Phe Glu Gln Ser Gln Met Gly Pro His Phe Gly Gln Gln His 
65                  70                  75                  80  


Phe Gly Gln Pro His Gln Pro Gln Met Gly Gln His Ala Pro Met Ala 
                85                  90                  95      


His Gly Gln Gln Ser Asp Trp Ala Gln Ser Phe Ser Gln Leu Asn Leu 
            100                 105                 110         


Gly Pro Gln Thr Gly Pro Gln His Thr Gln Gln Ser Asn Trp Gly Gln 
        115                 120                 125             


Asp Phe Met Arg Gln Ser Pro Gln Ser His Gln Val Gln Pro Gln Met 
    130                 135                 140                 


Ala Asn Gly Val Met Gly Ser Met Ser Gly Met Ser Ser Phe Gly Pro 
145                 150                 155                 160 


Met Tyr Ser Asn Ser Gln Leu Met Asn Ser Thr Tyr Gly Leu Gln Thr 
                165                 170                 175     


Glu His Gln Gln Thr His Lys Thr Glu Thr Lys Ser Ser Gln Asp Ala 
            180                 185                 190         


Ala Phe Glu Ala Ala Phe Gly Ala Val Glu Glu Ser Ile Thr Lys Thr 
        195                 200                 205             


Ser Asp Lys Gly Lys Glu Val Glu Lys Asp Pro Met Glu Gln Thr Tyr 
    210                 215                 220                 


Arg Tyr Asp Gln Ala Asp Ala Leu Asn Arg Gln Ala Glu His Ile Ser 
225                 230                 235                 240 


Asp Asn Ile Ser Arg Glu Glu Val Asp Ile Lys Thr Asp Glu Asn Gly 
                245                 250                 255     


Glu Phe Ala Ser Ile Ala Arg Gln Ile Ala Ser Ser Leu Glu Glu Ala 
            260                 265                 270         


Asp Lys Ser Lys Phe Glu Lys Ser Thr Phe Met Asn Leu Met Arg Arg 
        275                 280                 285             


Ile Gly Asn His Glu Val Thr Leu Asp Gly Asp Lys Leu Val Asn Lys 
    290                 295                 300                 


Glu Gly Glu Asp Ile Arg Glu Glu Val Arg Asp Glu Leu Leu Arg Glu 
305                 310                 315                 320 


Gly Ala Ser Gln Glu Asn Gly Phe Gln Ser Glu Ala Gln Gln Thr Ala 
                325                 330                 335     


Pro Leu Pro Val His His Glu Ala Pro Pro Pro Glu Gln Ile His Pro 
            340                 345                 350         


His Thr Glu Thr Gly Asp Lys Gln Leu Glu Asp Pro Met Val Tyr Ile 
        355                 360                 365             


Glu Gln Glu Ala Ala Arg Arg Ala Ala Glu Ser Gly Arg Thr Val Glu 
    370                 375                 380                 


Glu Glu Lys Leu Asn Phe Tyr Ser Pro Phe Glu Tyr Ala Gln Lys Leu 
385                 390                 395                 400 


Gly Pro Gln Gly Val Ala Lys Gln Ser Asn Trp Glu Glu Asp Tyr Asp 
                405                 410                 415     


Phe 
    


<210>  18
<211>  195
<212>  PRT
<213>  Yarrowia lipolytica CLIB122 (GenBank Accession No. CAG77876)


<220>
<221>  MISC_FEATURE
<222>  (1)..(195)
<223>  YlPex22p

<400>  18

Val Pro Arg Cys Thr Ser His Pro Cys Asn Leu Thr Leu His Leu Pro 
1               5                   10                  15      


Val Thr Thr Met Ala Pro Arg Lys Thr Arg Leu Pro Ala Val Ile Gly 
            20                  25                  30          


Ala Ala Ala Ala Ala Ala Ala Val Ala Tyr Leu Val Tyr Ser Phe Val 
        35                  40                  45              


Ala Lys Ser Asn Ser Asp Gln Asp Thr Phe Asp Ser Ser Val Gln Ser 
    50                  55                  60                  


Ser Ser Lys Ser Ser Thr Lys Ser Pro Lys Ser Thr Ala Thr Asn Ser 
65                  70                  75                  80  


Lys Ile Thr Val Val Val Ser Gln Glu Leu Val Gln Ser Gln Leu Val 
                85                  90                  95      


Asp Phe Lys His Leu Met Ser Val His Pro Asn Leu Val Val Ile Val 
            100                 105                 110         


Pro Pro Met Val Ala Asn Lys Phe His Arg Ala Leu Lys Ser Ser Val 
        115                 120                 125             


Gly His Asp His Gly Val Lys Val Ile Arg Cys Asp Thr Asp Val Gly 
    130                 135                 140                 


Val Ile His Val Ile Lys His Ile Arg Pro Asp Leu Ala Leu Ile Ala 
145                 150                 155                 160 


Asp Gly Val Gly Asp Asn Ile Gln Gly Glu Ile Lys Arg Phe Val Gly 
                165                 170                 175     


Ser Ser Glu Ala Leu Ser Gly Asp Val Asn Leu Ala Ala Glu Arg Leu 
            180                 185                 190         


Thr Gly Leu 
        195 


<210>  19
<211>  386
<212>  PRT
<213>  Yarrowia lipolytica CLIB122 (GenBank Accession No. NC_006072, antisense translation of nucleotides 117230-118387)


<220>
<221>  MISC_FEATURE
<222>  (1)..(386)
<223>  YlPex26p

<400>  19

Met Pro Pro Ala Met Pro Gln Met Thr Thr Ser Thr Leu Leu Thr Asp 
1               5                   10                  15      


Ser Val Thr Ser Ala Val Asn Gln Ala Ala Thr Pro Lys Val Asp Gln 
            20                  25                  30          


Met Tyr Gln Thr Phe Gly Glu Ser Ala Arg Glu Phe Val Asn Lys Asn 
        35                  40                  45              


Phe Tyr Asn Ser Tyr Glu Leu Ile Arg Pro Phe Phe Asp Glu Ile Thr 
    50                  55                  60                  


Ala Lys Gly Ala Gln Gln Asn Gly Ser Thr Val Leu Asp Ala Glu Asn 
65                  70                  75                  80  


Pro His Asn Ile Pro Leu Ser Leu Trp Ile Lys Val Trp Ser Leu Tyr 
                85                  90                  95      


Leu Ala Ile Leu Asp Ala Ser Cys Lys Gln Ala Gly Glu Ala Leu Leu 
            100                 105                 110         


Asn Ser Thr Gly Asp Leu Ser Gly Ser Asp Ser Gly Glu Trp Asn Gln 
        115                 120                 125             


Thr Arg Lys Leu Leu Ala Arg Lys Leu Thr Ser Gly Ser Val Trp Asp 
    130                 135                 140                 


Glu Leu Val Thr Ala Ser Gly Gly Thr Gly Asn Ile His Pro Thr Ile 
145                 150                 155                 160 


Leu Ala Leu Leu Ala Ser Leu Ser Ile Arg His Asp Thr Asp Ala Lys 
                165                 170                 175     


Leu Met Ala Asp Asn Leu Glu Lys Phe Ile Val Thr Tyr Asn Asp Asn 
            180                 185                 190         


Gly Ser Asp Asp Val Lys Thr Lys Thr Ala Phe Tyr Lys Val Leu Asp 
        195                 200                 205             


Leu Tyr Leu Leu Arg Val Leu Pro Asp Leu Gly Gln Trp Asp Val Ala 
    210                 215                 220                 


His Ser Phe Val Asn Asn Thr Asn Leu Phe Ser His Glu Gln Lys Lys 
225                 230                 235                 240 


Glu Met Thr His Lys Leu Asp Gln Ser Gln Lys His Ala Glu Gln Glu 
                245                 250                 255     


His Lys Arg Leu Leu Glu Glu Ala Gln Glu Lys Glu Lys Ser Asp Ala 
            260                 265                 270         


Lys Glu Lys Glu Arg Glu Glu Arg Val Ser Arg Asp Thr Gln Ser Arg 
        275                 280                 285             


Glu Ile Lys Ser Pro Ile Val Asp Ser Ser Thr Ser Ser Arg Asp Val 
    290                 295                 300                 


Thr Arg Asp Thr Thr Arg Glu Leu Ser Lys Ser Ser Arg Gln Pro Arg 
305                 310                 315                 320 


Thr Leu Ser Gln Ile Ile Ser Thr Ser Leu Lys Ser Gln Phe Asp Gly 
                325                 330                 335     


Asn Ala Ile Phe Arg Thr Leu Ala Leu Ile Val Ile Val Ser Leu Ser 
            340                 345                 350         


Ala Ala Asn Pro Leu Ile Arg Lys Arg Val Val Asp Thr Leu Lys Met 
        355                 360                 365             


Leu Trp Ile Lys Ile Leu Gln Thr Leu Ser Met Gly Phe Lys Val Ser 
    370                 375                 380                 


Tyr Leu 
385     


<210>  20
<211>  3387
<212>  DNA
<213>  Yarrowia lipolytica (GenBank Accession No. AB036770)

<400>  20
ggtaccatca agggtaaaat caaggctatc atcaagggcc atatatcgca agtttggggg     60

aagataatat gttcatagtg aatcgggttg tggatttcct catctaacgg cattataact    120

agtcctggag ggtctttttt atggataacc tccatgtacg atgtatccaa gatctccacg    180

tactgtgttc tgtttcctaa gtaataccca acaacctctc caacaaacac ttgggaagat    240

gcacttgtgc tgagatgtca agatgttaga gagtagagac agtagcaagc gtaaaaggcg    300

gccgaggcca ccgagagaac agcgtagcag ggcgcgtagt caccacaggg gacgcagaac    360

caaacaaatg acgaagaaga accacaagga gacgttttca aaggcaatgc aaacgaagag    420

ggcaatggaa ggattgagat tagagaactg gagactggag tggcgttttc ccgatgaacg    480

aacaaacacg cgaagctatg tggaccaaca tacaacacgg actgaaccag gtttttttat    540

gattttttta ctggaaatag gtacgtgcca agttggacca tgacactaaa cgtgtttaat    600

tagtaatatt cgtgtaagcg tacattcatt tcaaaggtta ttctttcacg gcaaagttat    660

aattaaatga atgtatatgc agaaaaaaaa aaaaaaagta ctgtactgga tggagagaat    720

attaataaat aattgttacc caactacatc ttgtcgattg aaagagaccc ctaagacaga    780

taggatatct gcaacccgag gaatgaaccc cccagcaccg gcaccctttc tattaacaaa    840

atgccaactg aaatttgaaa agttcaacta aacttatttg acccacaaaa actcgtcaaa    900

agtggcggcg aaagctggca aatgatgaca tccccttgga accatgatat cctctcggaa    960

tcttcgtccc catttgccac atctacttgc aacgccacat ctgcttacta agcaacccaa   1020

atctgcctcg gctcaaaatg tggggaagtt cacatgcatt cgctggtgaa tctgatctga   1080

cactacaact acacaccagg tccaacatga gcgacaatac gacaatcaaa aagccgatcc   1140

gacccaaacc gatccggacg gaacgcctgc cttacgctgg ggccgcagaa atcatccgag   1200

ccaaccagaa agaccactac tttgagtccg tgcttgaaca gcatctcgtc acgtttctgc   1260

agaaatggaa gggagtacga tttatccacc agtacaagga ggagctggag acggcgtcca   1320

agtttgcata tctcggtttg tgtacgcttg tgggctccaa gactctcgga gaagagtaca   1380

ccaatctcat gtacactatc agagaccgaa cagctctacc gggggtggtg agacggtttg   1440

gctacgtgct ttccaacact ctgtttccat acctgtttgt gcgctacatg ggcaagttgc   1500

gcgccaaact gatgcgcgag tatccccatc tggtggagta cgacgaagat gagcctgtgc   1560

ccagcccgga aacatggaag gagcgggtca tcaagacgtt tgtgaacaag tttgacaagt   1620

tcacggcgct ggaggggttt accgcgatcc acttggcgat tttctacgtc tacggctcgt   1680

actaccagct cagtaagcgg atctggggca tgcgttatgt atttggacac cgactggaca   1740

agaatgagcc tcgaatcggt tacgagatgc tcggtctgct gattttcgcc cggtttgcca   1800

cgtcatttgt gcagacggga agagagtacc tcggagcgct gctggaaaag agcgtggaga   1860

aagaggcagg ggagaaggaa gatgaaaagg aagcggttgt gccgaaaaag aagtcgtcaa   1920

ttccgttcat tgaggataca gaaggggaga cggaagacaa gatcgatctg gaggaccctc   1980

gacagctcaa gttcattcct gaggcgtcca gagcgtgcac tctgtgtctg tcatacatta   2040

gtgcgccggc atgtacgcca tgtggacact ttttctgttg ggactgtatt tccgaatggg   2100

tgagagagaa gcccgagtgt cccttgtgtc ggcagggtgt gagagagcag aacttgttgc   2160

ctatcagata atgacgaggt ctggatggaa ggactagtca gcgagacaca gagcatcagg   2220

gaccagacac gaccaattca atcgacaaca ctgtgctgca tagcagtgca cagaggtcct   2280

gggcatgaat atattttagc attggagata tgagtggtag agcgtataca gtattaattg   2340

tggaggtatc tcgtcgcatt gatagagcaa tacagttact gctgaaggga atgataccga   2400

gtatttcggc ccgattcagt tcttgatatc gtcattttgt ctctattgtc tacttttcag   2460

ataacctcaa caaatcttca acaaatctcc cagtaaacag tcagagatca tatccgagat   2520

catatcagat atgtcacgat ccgagtacaa taatggatat taatctgctt gattttgaat   2580

tctgttgcga ttatgatttc tttgatttcg atatgaacac atacggcgac tcccagacct   2640

ttagaagctc cagtttggat tcttagcaat ggttacactc aactatatcc caagtaatac   2700

ttggtaacaa tatgccaagt tagtcattca ttcgttatag gagttagcaa gtgtttgtca   2760

gctaaaaatg gttagtcggt cgattaccac ttagatcttt tcagcgtgga acttgatggt   2820

acgcttgaac cgacacttgg agtagtcggg gctgttgatg acgtagatga cgtttcgctc   2880

agggtgagga gtgcaatagt agtactcctt ggggccgtct ctcagctcaa aggttccatc   2940

ggcggcaatg tcaaagaccg agccctggag cttgtagccg tagtcgccgg tccagaacaa   3000

agcctgcagc tccagatagg cgatgggcat gtcgttaaca gagaaggtgt tgccctcgcc   3060

ctcggtgatg gtgatgggtt cgccgtcggt ggaggcggtg atcaggtcat cttggtaggt   3120

gacgggcaga gattcgaccg attgggcgtc tgatctggta taggtcagct tgtacttgtc   3180

tccgacagcc gccagagcgg tggtagcgac ggtgatgagg gagatgagtt tcatattggc   3240

ggcaagttta gcaaaagatg gcagtgggat tgagggacaa gagtgtttat atagatatag   3300

atacaacaca acgagtctga atgagacaac cgagacaacc actcccgaag cctcactaat   3360

agttactaac ggcatatttc aggtacc                                       3387


<210>  21
<211>  1134
<212>  DNA
<213>  Yarrowia lipolytica (GenBank Accession No. AB036770, nucleotides 1038-2171)


<220>
<221>  CDS
<222>  (1)..(1134)
<223>  Pex10

<400>  21
atg tgg gga agt tca cat gca ttc gct ggt gaa tct gat ctg aca cta       48
Met Trp Gly Ser Ser His Ala Phe Ala Gly Glu Ser Asp Leu Thr Leu         
1               5                   10                  15              

caa cta cac acc agg tcc aac atg agc gac aat acg aca atc aaa aag       96
Gln Leu His Thr Arg Ser Asn Met Ser Asp Asn Thr Thr Ile Lys Lys         
            20                  25                  30                  

ccg atc cga ccc aaa ccg atc cgg acg gaa cgc ctg cct tac gct ggg      144
Pro Ile Arg Pro Lys Pro Ile Arg Thr Glu Arg Leu Pro Tyr Ala Gly         
        35                  40                  45                      

gcc gca gaa atc atc cga gcc aac cag aaa gac cac tac ttt gag tcc      192
Ala Ala Glu Ile Ile Arg Ala Asn Gln Lys Asp His Tyr Phe Glu Ser         
    50                  55                  60                          

gtg ctt gaa cag cat ctc gtc acg ttt ctg cag aaa tgg aag gga gta      240
Val Leu Glu Gln His Leu Val Thr Phe Leu Gln Lys Trp Lys Gly Val         
65                  70                  75                  80          

cga ttt atc cac cag tac aag gag gag ctg gag acg gcg tcc aag ttt      288
Arg Phe Ile His Gln Tyr Lys Glu Glu Leu Glu Thr Ala Ser Lys Phe         
                85                  90                  95              

gca tat ctc ggt ttg tgt acg ctt gtg ggc tcc aag act ctc gga gaa      336
Ala Tyr Leu Gly Leu Cys Thr Leu Val Gly Ser Lys Thr Leu Gly Glu         
            100                 105                 110                 

gag tac acc aat ctc atg tac act atc aga gac cga aca gct cta ccg      384
Glu Tyr Thr Asn Leu Met Tyr Thr Ile Arg Asp Arg Thr Ala Leu Pro         
        115                 120                 125                     

ggg gtg gtg aga cgg ttt ggc tac gtg ctt tcc aac act ctg ttt cca      432
Gly Val Val Arg Arg Phe Gly Tyr Val Leu Ser Asn Thr Leu Phe Pro         
    130                 135                 140                         

tac ctg ttt gtg cgc tac atg ggc aag ttg cgc gcc aaa ctg atg cgc      480
Tyr Leu Phe Val Arg Tyr Met Gly Lys Leu Arg Ala Lys Leu Met Arg         
145                 150                 155                 160         

gag tat ccc cat ctg gtg gag tac gac gaa gat gag cct gtg ccc agc      528
Glu Tyr Pro His Leu Val Glu Tyr Asp Glu Asp Glu Pro Val Pro Ser         
                165                 170                 175             

ccg gaa aca tgg aag gag cgg gtc atc aag acg ttt gtg aac aag ttt      576
Pro Glu Thr Trp Lys Glu Arg Val Ile Lys Thr Phe Val Asn Lys Phe         
            180                 185                 190                 

gac aag ttc acg gcg ctg gag ggg ttt acc gcg atc cac ttg gcg att      624
Asp Lys Phe Thr Ala Leu Glu Gly Phe Thr Ala Ile His Leu Ala Ile         
        195                 200                 205                     

ttc tac gtc tac ggc tcg tac tac cag ctc agt aag cgg atc tgg ggc      672
Phe Tyr Val Tyr Gly Ser Tyr Tyr Gln Leu Ser Lys Arg Ile Trp Gly         
    210                 215                 220                         

atg cgt tat gta ttt gga cac cga ctg gac aag aat gag cct cga atc      720
Met Arg Tyr Val Phe Gly His Arg Leu Asp Lys Asn Glu Pro Arg Ile         
225                 230                 235                 240         

ggt tac gag atg ctc ggt ctg ctg att ttc gcc cgg ttt gcc acg tca      768
Gly Tyr Glu Met Leu Gly Leu Leu Ile Phe Ala Arg Phe Ala Thr Ser         
                245                 250                 255             

ttt gtg cag acg gga aga gag tac ctc gga gcg ctg ctg gaa aag agc      816
Phe Val Gln Thr Gly Arg Glu Tyr Leu Gly Ala Leu Leu Glu Lys Ser         
            260                 265                 270                 

gtg gag aaa gag gca ggg gag aag gaa gat gaa aag gaa gcg gtt gtg      864
Val Glu Lys Glu Ala Gly Glu Lys Glu Asp Glu Lys Glu Ala Val Val         
        275                 280                 285                     

ccg aaa aag aag tcg tca att ccg ttc att gag gat aca gaa ggg gag      912
Pro Lys Lys Lys Ser Ser Ile Pro Phe Ile Glu Asp Thr Glu Gly Glu         
    290                 295                 300                         

acg gaa gac aag atc gat ctg gag gac cct cga cag ctc aag ttc att      960
Thr Glu Asp Lys Ile Asp Leu Glu Asp Pro Arg Gln Leu Lys Phe Ile         
305                 310                 315                 320         

cct gag gcg tcc aga gcg tgc act ctg tgt ctg tca tac att agt gcg     1008
Pro Glu Ala Ser Arg Ala Cys Thr Leu Cys Leu Ser Tyr Ile Ser Ala         
                325                 330                 335             

ccg gca tgt acg cca tgt gga cac ttt ttc tgt tgg gac tgt att tcc     1056
Pro Ala Cys Thr Pro Cys Gly His Phe Phe Cys Trp Asp Cys Ile Ser         
            340                 345                 350                 

gaa tgg gtg aga gag aag ccc gag tgt ccc ttg tgt cgg cag ggt gtg     1104
Glu Trp Val Arg Glu Lys Pro Glu Cys Pro Leu Cys Arg Gln Gly Val         
        355                 360                 365                     

aga gag cag aac ttg ttg cct atc aga taa                             1134
Arg Glu Gln Asn Leu Leu Pro Ile Arg                                     
    370                 375                                             


<210>  22
<211>  377
<212>  PRT
<213>  Yarrowia lipolytica (GenBank Accession No. AB036770, nucleotides 1038-2171)

<400>  22

Met Trp Gly Ser Ser His Ala Phe Ala Gly Glu Ser Asp Leu Thr Leu 
1               5                   10                  15      


Gln Leu His Thr Arg Ser Asn Met Ser Asp Asn Thr Thr Ile Lys Lys 
            20                  25                  30          


Pro Ile Arg Pro Lys Pro Ile Arg Thr Glu Arg Leu Pro Tyr Ala Gly 
        35                  40                  45              


Ala Ala Glu Ile Ile Arg Ala Asn Gln Lys Asp His Tyr Phe Glu Ser 
    50                  55                  60                  


Val Leu Glu Gln His Leu Val Thr Phe Leu Gln Lys Trp Lys Gly Val 
65                  70                  75                  80  


Arg Phe Ile His Gln Tyr Lys Glu Glu Leu Glu Thr Ala Ser Lys Phe 
                85                  90                  95      


Ala Tyr Leu Gly Leu Cys Thr Leu Val Gly Ser Lys Thr Leu Gly Glu 
            100                 105                 110         


Glu Tyr Thr Asn Leu Met Tyr Thr Ile Arg Asp Arg Thr Ala Leu Pro 
        115                 120                 125             


Gly Val Val Arg Arg Phe Gly Tyr Val Leu Ser Asn Thr Leu Phe Pro 
    130                 135                 140                 


Tyr Leu Phe Val Arg Tyr Met Gly Lys Leu Arg Ala Lys Leu Met Arg 
145                 150                 155                 160 


Glu Tyr Pro His Leu Val Glu Tyr Asp Glu Asp Glu Pro Val Pro Ser 
                165                 170                 175     


Pro Glu Thr Trp Lys Glu Arg Val Ile Lys Thr Phe Val Asn Lys Phe 
            180                 185                 190         


Asp Lys Phe Thr Ala Leu Glu Gly Phe Thr Ala Ile His Leu Ala Ile 
        195                 200                 205             


Phe Tyr Val Tyr Gly Ser Tyr Tyr Gln Leu Ser Lys Arg Ile Trp Gly 
    210                 215                 220                 


Met Arg Tyr Val Phe Gly His Arg Leu Asp Lys Asn Glu Pro Arg Ile 
225                 230                 235                 240 


Gly Tyr Glu Met Leu Gly Leu Leu Ile Phe Ala Arg Phe Ala Thr Ser 
                245                 250                 255     


Phe Val Gln Thr Gly Arg Glu Tyr Leu Gly Ala Leu Leu Glu Lys Ser 
            260                 265                 270         


Val Glu Lys Glu Ala Gly Glu Lys Glu Asp Glu Lys Glu Ala Val Val 
        275                 280                 285             


Pro Lys Lys Lys Ser Ser Ile Pro Phe Ile Glu Asp Thr Glu Gly Glu 
    290                 295                 300                 


Thr Glu Asp Lys Ile Asp Leu Glu Asp Pro Arg Gln Leu Lys Phe Ile 
305                 310                 315                 320 


Pro Glu Ala Ser Arg Ala Cys Thr Leu Cys Leu Ser Tyr Ile Ser Ala 
                325                 330                 335     


Pro Ala Cys Thr Pro Cys Gly His Phe Phe Cys Trp Asp Cys Ile Ser 
            340                 345                 350         


Glu Trp Val Arg Glu Lys Pro Glu Cys Pro Leu Cys Arg Gln Gly Val 
        355                 360                 365             


Arg Glu Gln Asn Leu Leu Pro Ile Arg 
    370                 375         


<210>  23
<211>  1065
<212>  DNA
<213>  Yarrowia lipolytica (GenBank Accession No. AJ012084, which corresponds to nucleotides 1107-2171 of GenBank Accession No. AB036770)


<220>
<221>  CDS
<222>  (1)..(1065)
<223>  YlPEX10

<400>  23
atg agc gac aat acg aca atc aaa aag ccg atc cga ccc aaa ccg atc       48
Met Ser Asp Asn Thr Thr Ile Lys Lys Pro Ile Arg Pro Lys Pro Ile         
1               5                   10                  15              

cgg acg gaa cgc ctg cct tac gct ggg gcc gca gaa atc atc cga gcc       96
Arg Thr Glu Arg Leu Pro Tyr Ala Gly Ala Ala Glu Ile Ile Arg Ala         
            20                  25                  30                  

aac cag aaa gac cac tac ttt gag tcc gtg ctt gaa cag cat ctc gtc      144
Asn Gln Lys Asp His Tyr Phe Glu Ser Val Leu Glu Gln His Leu Val         
        35                  40                  45                      

acg ttt ctg cag aaa tgg aag gga gta cga ttt atc cac cag tac aag      192
Thr Phe Leu Gln Lys Trp Lys Gly Val Arg Phe Ile His Gln Tyr Lys         
    50                  55                  60                          

gag gag ctg gag acg gcg tcc aag ttt gca tat ctc ggt ttg tgt acg      240
Glu Glu Leu Glu Thr Ala Ser Lys Phe Ala Tyr Leu Gly Leu Cys Thr         
65                  70                  75                  80          

ctt gtg ggc tcc aag act ctc gga gaa gag tac acc aat ctc atg tac      288
Leu Val Gly Ser Lys Thr Leu Gly Glu Glu Tyr Thr Asn Leu Met Tyr         
                85                  90                  95              

act atc aga gac cga aca gct cta ccg ggg gtg gtg aga cgg ttt ggc      336
Thr Ile Arg Asp Arg Thr Ala Leu Pro Gly Val Val Arg Arg Phe Gly         
            100                 105                 110                 

tac gtg ctt tcc aac act ctg ttt cca tac ctg ttt gtg cgc tac atg      384
Tyr Val Leu Ser Asn Thr Leu Phe Pro Tyr Leu Phe Val Arg Tyr Met         
        115                 120                 125                     

ggc aag ttg cgc gcc aaa ctg atg cgc gag tat ccc cat ctg gtg gag      432
Gly Lys Leu Arg Ala Lys Leu Met Arg Glu Tyr Pro His Leu Val Glu         
    130                 135                 140                         

tac gac gaa gat gag cct gtg ccc agc ccg gaa aca tgg aag gag cgg      480
Tyr Asp Glu Asp Glu Pro Val Pro Ser Pro Glu Thr Trp Lys Glu Arg         
145                 150                 155                 160         

gtc atc aag acg ttt gtg aac aag ttt gac aag ttc acg gcg ctg gag      528
Val Ile Lys Thr Phe Val Asn Lys Phe Asp Lys Phe Thr Ala Leu Glu         
                165                 170                 175             

ggg ttt acc gcg atc cac ttg gcg att ttc tac gtc tac ggc tcg tac      576
Gly Phe Thr Ala Ile His Leu Ala Ile Phe Tyr Val Tyr Gly Ser Tyr         
            180                 185                 190                 

tac cag ctc agt aag cgg atc tgg ggc atg cgt tat gta ttt gga cac      624
Tyr Gln Leu Ser Lys Arg Ile Trp Gly Met Arg Tyr Val Phe Gly His         
        195                 200                 205                     

cga ctg gac aag aat gag cct cga atc ggt tac gag atg ctc ggt ctg      672
Arg Leu Asp Lys Asn Glu Pro Arg Ile Gly Tyr Glu Met Leu Gly Leu         
    210                 215                 220                         

ctg att ttc gcc cgg ttt gcc acg tca ttt gtg cag acg gga aga gag      720
Leu Ile Phe Ala Arg Phe Ala Thr Ser Phe Val Gln Thr Gly Arg Glu         
225                 230                 235                 240         

tac ctc gga gcg ctg ctg gaa aag agc gtg gag aaa gag gca ggg gag      768
Tyr Leu Gly Ala Leu Leu Glu Lys Ser Val Glu Lys Glu Ala Gly Glu         
                245                 250                 255             

aag gaa gat gaa aag gaa gcg gtt gtg ccg aaa aag aag tcg tca att      816
Lys Glu Asp Glu Lys Glu Ala Val Val Pro Lys Lys Lys Ser Ser Ile         
            260                 265                 270                 

ccg ttc att gag gat aca gaa ggg gag acg gaa gac aag atc gat ctg      864
Pro Phe Ile Glu Asp Thr Glu Gly Glu Thr Glu Asp Lys Ile Asp Leu         
        275                 280                 285                     

gag gac cct cga cag ctc aag ttc att cct gag gcg tcc aga gcg tgc      912
Glu Asp Pro Arg Gln Leu Lys Phe Ile Pro Glu Ala Ser Arg Ala Cys         
    290                 295                 300                         

act ctg tgt ctg tca tac att agt gcg ccg gca tgt acg cca tgt gga      960
Thr Leu Cys Leu Ser Tyr Ile Ser Ala Pro Ala Cys Thr Pro Cys Gly         
305                 310                 315                 320         

cac ttt ttc tgt tgg gac tgt att tcc gaa tgg gtg aga gag aag ccc     1008
His Phe Phe Cys Trp Asp Cys Ile Ser Glu Trp Val Arg Glu Lys Pro         
                325                 330                 335             

gag tgt ccc ttg tgt cgg cag ggt gtg aga gag cag aac ttg ttg cct     1056
Glu Cys Pro Leu Cys Arg Gln Gly Val Arg Glu Gln Asn Leu Leu Pro         
            340                 345                 350                 

atc aga taa                                                         1065
Ile Arg                                                                 
                                                                        


<210>  24
<211>  354
<212>  PRT
<213>  Yarrowia lipolytica (GenBank Accession No. AJ012084, which corresponds to nucleotides 1107-2171 of GenBank Accession No. AB036770)

<400>  24

Met Ser Asp Asn Thr Thr Ile Lys Lys Pro Ile Arg Pro Lys Pro Ile 
1               5                   10                  15      


Arg Thr Glu Arg Leu Pro Tyr Ala Gly Ala Ala Glu Ile Ile Arg Ala 
            20                  25                  30          


Asn Gln Lys Asp His Tyr Phe Glu Ser Val Leu Glu Gln His Leu Val 
        35                  40                  45              


Thr Phe Leu Gln Lys Trp Lys Gly Val Arg Phe Ile His Gln Tyr Lys 
    50                  55                  60                  


Glu Glu Leu Glu Thr Ala Ser Lys Phe Ala Tyr Leu Gly Leu Cys Thr 
65                  70                  75                  80  


Leu Val Gly Ser Lys Thr Leu Gly Glu Glu Tyr Thr Asn Leu Met Tyr 
                85                  90                  95      


Thr Ile Arg Asp Arg Thr Ala Leu Pro Gly Val Val Arg Arg Phe Gly 
            100                 105                 110         


Tyr Val Leu Ser Asn Thr Leu Phe Pro Tyr Leu Phe Val Arg Tyr Met 
        115                 120                 125             


Gly Lys Leu Arg Ala Lys Leu Met Arg Glu Tyr Pro His Leu Val Glu 
    130                 135                 140                 


Tyr Asp Glu Asp Glu Pro Val Pro Ser Pro Glu Thr Trp Lys Glu Arg 
145                 150                 155                 160 


Val Ile Lys Thr Phe Val Asn Lys Phe Asp Lys Phe Thr Ala Leu Glu 
                165                 170                 175     


Gly Phe Thr Ala Ile His Leu Ala Ile Phe Tyr Val Tyr Gly Ser Tyr 
            180                 185                 190         


Tyr Gln Leu Ser Lys Arg Ile Trp Gly Met Arg Tyr Val Phe Gly His 
        195                 200                 205             


Arg Leu Asp Lys Asn Glu Pro Arg Ile Gly Tyr Glu Met Leu Gly Leu 
    210                 215                 220                 


Leu Ile Phe Ala Arg Phe Ala Thr Ser Phe Val Gln Thr Gly Arg Glu 
225                 230                 235                 240 


Tyr Leu Gly Ala Leu Leu Glu Lys Ser Val Glu Lys Glu Ala Gly Glu 
                245                 250                 255     


Lys Glu Asp Glu Lys Glu Ala Val Val Pro Lys Lys Lys Ser Ser Ile 
            260                 265                 270         


Pro Phe Ile Glu Asp Thr Glu Gly Glu Thr Glu Asp Lys Ile Asp Leu 
        275                 280                 285             


Glu Asp Pro Arg Gln Leu Lys Phe Ile Pro Glu Ala Ser Arg Ala Cys 
    290                 295                 300                 


Thr Leu Cys Leu Ser Tyr Ile Ser Ala Pro Ala Cys Thr Pro Cys Gly 
305                 310                 315                 320 


His Phe Phe Cys Trp Asp Cys Ile Ser Glu Trp Val Arg Glu Lys Pro 
                325                 330                 335     


Glu Cys Pro Leu Cys Arg Gln Gly Val Arg Glu Gln Asn Leu Leu Pro 
            340                 345                 350         


Ile Arg 
        


<210>  25
<211>  38
<212>  PRT
<213>  Yarrowia lipolytica


<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (5)..(15)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (17)..(17)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (22)..(23)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (25)..(34)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (36)..(37)
<223>  Xaa can be any naturally occurring amino acid

<400>  25

Cys Xaa Xaa Cys Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Cys 
1               5                   10                  15      


Xaa His Xaa Xaa Cys Xaa Xaa Cys Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
            20                  25                  30          


Xaa Xaa Cys Xaa Xaa Cys 
        35              


<210>  26
<211>  345
<212>  PRT
<213>  Yarrowia lipolytica 

<400>  26

Met Trp Gly Ser Ser His Ala Phe Ala Gly Glu Ser Asp Leu Thr Leu 
1               5                   10                  15      


Gln Leu His Thr Arg Ser Asn Met Ser Asp Asn Thr Thr Ile Lys Lys 
            20                  25                  30          


Pro Ile Arg Pro Lys Pro Ile Arg Thr Glu Arg Leu Pro Tyr Ala Gly 
        35                  40                  45              


Ala Ala Glu Ile Ile Arg Ala Asn Gln Lys Asp His Tyr Phe Glu Ser 
    50                  55                  60                  


Val Leu Glu Gln His Leu Val Thr Phe Leu Gln Lys Trp Lys Gly Val 
65                  70                  75                  80  


Arg Phe Ile His Gln Tyr Lys Glu Glu Leu Glu Thr Ala Ser Lys Phe 
                85                  90                  95      


Ala Tyr Leu Gly Leu Cys Thr Leu Val Gly Ser Lys Thr Leu Gly Glu 
            100                 105                 110         


Glu Tyr Thr Asn Leu Met Tyr Thr Ile Arg Asp Arg Thr Ala Leu Pro 
        115                 120                 125             


Gly Val Val Arg Arg Phe Gly Tyr Val Leu Ser Asn Thr Leu Phe Pro 
    130                 135                 140                 


Tyr Leu Phe Val Arg Tyr Met Gly Lys Leu Arg Ala Lys Leu Met Arg 
145                 150                 155                 160 


Glu Tyr Pro His Leu Val Glu Tyr Asp Glu Asp Glu Pro Val Pro Ser 
                165                 170                 175     


Pro Glu Thr Trp Lys Glu Arg Val Ile Lys Thr Phe Val Asn Lys Phe 
            180                 185                 190         


Asp Lys Phe Thr Ala Leu Glu Gly Phe Thr Ala Ile His Leu Ala Ile 
        195                 200                 205             


Phe Tyr Val Tyr Gly Ser Tyr Tyr Gln Leu Ser Lys Arg Ile Trp Gly 
    210                 215                 220                 


Met Arg Tyr Val Phe Gly His Arg Leu Asp Lys Asn Glu Pro Arg Ile 
225                 230                 235                 240 


Gly Tyr Glu Met Leu Gly Leu Leu Ile Phe Ala Arg Phe Ala Thr Ser 
                245                 250                 255     


Phe Val Gln Thr Gly Arg Glu Tyr Leu Gly Ala Leu Leu Glu Lys Ser 
            260                 265                 270         


Val Glu Lys Glu Ala Gly Glu Lys Glu Asp Glu Lys Glu Ala Val Val 
        275                 280                 285             


Pro Lys Lys Lys Ser Ser Ile Pro Phe Ile Glu Asp Thr Glu Gly Glu 
    290                 295                 300                 


Thr Glu Asp Lys Ile Asp Leu Glu Asp Pro Arg Gln Leu Lys Phe Ile 
305                 310                 315                 320 


Pro Glu Ala Ser Arg Ala Cys Thr Leu Cys Leu Ser Tyr Ile Ser Ala 
                325                 330                 335     


Pro Ala Cys Thr Pro Cys Gly His Phe 
            340                 345 


<210>  27
<211>  2987
<212>  DNA
<213>  Yarrowia lipolytica


<220>
<221>  misc_feature
<223>  mutant acetohydroxyacid synthase (AHAS) with W497L mutation

<300>
<302>  HIGH EICOSAPENTAENOIC ACID PRODUCING STRAINS OF YARROWIA 
       LIPOLYTICA
<310>  US 2006-0115881-A1
<311>  2005-11-02
<312>  2006-06-01
<313>  (1)..(2987)

<300>
<302>  HIGH EICOSAPENTAENOIC ACID PRODUCING STRAINS OF YARROWIA 
       LIPOLYTICA
<310>  WO 2006/052870
<311>  2005-11-03
<312>  2006-05-18
<313>  (1)..(2987)

<400>  27
ttccctagtc ccagtgtaca cccgccgata tcgcttaccc tgcagccgga ttaaggttgg     60

caatttttca cgtccttgtc tccgcaatta ctcaccgggt ggtttataag attgcaagcg    120

tcttgatttg tctctgtata ctaacatgca atcgcgactc gcccgacggg ccactaacct    180

ggccagaatc tccagatcca agtattctct tggtctgcga tatgtttcca acacaaaagc    240

ccctgctgcc cagccggcaa ctgctgagtg agtattcctt gccataaacg acccagaacc    300

actgtatagt gtttggaagc actagtcaga agaccagcga aaacaggtgg aaaaaactga    360

gacgaaaagc aacgaccaga aatgtaatgt gtggaaaagc gacacacaca gagcagataa    420

agaggtgaca aataacgaca aatgaaatat cagtatcttc ccacaatcac tacctctcag    480

ctgtctgaag gtgcggctga tatatccatc ccacgtctaa cgtatggagt gtgatagaat    540

atgacgacac aagcatgaga actcgctctc tatccaacca ccgaaacact gtcactacag    600

ccgttcttgt tgctccattc gcttttgtga ttccatgcct tctctggtga ctgacaacat    660

tccttccttt tctccagccc tgttgttatc tgctcatgac ctacggccac tctctatcgc    720

atactaacat agacgatccc agcccgctcc ccacttccag ggcaccgttg gcaagcctcc    780

tatcctcaag aaggctgagg ctgccaacgc tgacatggac gagtccttca tcggaatgtc    840

tggaggagag atcttccacg agatgatgct gcgacacaac gtcgacactg tcttcggtta    900

ccccggtgga gccattctcc ccgtctttga cgccattcac aactctgagt acttcaactt    960

tgtgctccct cgacacgagc agggtgccgg ccacatggcc gagggctacg ctcgagcctc   1020

tggtaagccc ggtgtcgttc tcgtcacctc tggccccggt gccaccaacg tcatcacccc   1080

catgcaggac gctctttccg atggtacccc catggttgtc ttcaccggtc aggtcctgac   1140

ctccgttatc ggcactgacg ccttccagga ggccgatgtt gtcggcatct cccgatcttg   1200

caccaagtgg aacgtcatgg tcaagaacgt tgctgagctc ccccgacgaa tcaacgaggc   1260

ctttgagatt gctacttccg gccgacccgg tcccgttctc gtcgatctgc ccaaggatgt   1320

tactgctgcc atcctgcgag agcccatccc caccaagtcc accattccct cgcattctct   1380

gaccaacctc acctctgccg ccgccaccga gttccagaag caggctatcc agcgagccgc   1440

caacctcatc aaccagtcca agaagcccgt cctttacgtc ggacagggta tccttggctc   1500

cgaggagggt cctaagctgc ttaaggagct ggctgagaag gccgagattc ccgtcaccac   1560

tactctgcag ggtcttggtg cctttgacga gcgagacccc aagtctctgc acatgctcgg   1620

tatgcacggt tccggctacg ccaacatggc catgcagaac gctgactgta tcattgctct   1680

cggcgcccga tttgatgacc gagttaccgg ctccatcccc aagtttgccc ccgaggctcg   1740

agccgctgcc cttgagggtc gaggtggtat tgttcacttt gagatccagg ccaagaacat   1800

caacaaggtt gttcaggcca ccgaagccgt tgagggagac gttaccgagt ctgtccgaca   1860

gctcatcccc ctcatcaaca aggtctctgc cgctgagcga gctccctgga ctgagactat   1920

ccagtcctgg aagcagcagt tccccttcct cttcgaggct gaaggtgagg atggtgttat   1980

caagccccag tccgtcattg ctctgctctc tgacctgaca gagaacaaca aggacaagac   2040

catcatcacc accggtgttg gtcagcatca gatgtggact gcccagcatt tccgatggcg   2100

acaccctcga accatgatca cttctggtgg tcttggaact atgggttacg gcctgcccgc   2160

cgctatcggc gccaaggttg cccgacctga ctgcgacgtc attgacatcg atggtgacgc   2220

ttctttcaac atgactctga ccgagctgtc caccgccgtt cagttcaaca ttggcgtcaa   2280

ggctattgtc ctcaacaacg aggaacaggg tatggtcacc cagctgcagt ctctcttcta   2340

cgagaaccga tactgccaca ctcatcagaa gaaccccgac ttcatgaagc tggccgagtc   2400

catgggcatg aagggtatcc gaatcactca cattgaccag ctggaggccg gtctcaagga   2460

gatgctcgca tacaagggcc ctgtgctcgt tgaggttgtt gtcgacaaga agatccccgt   2520

tcttcccatg gttcccgctg gtaaggcttt gcatgagttc cttgtctacg acgctgacgc   2580

cgaggctgct tctcgacccg atcgactgaa gaatgccccc gcccctcacg tccaccagac   2640

cacctttgag aactaagtgg aaaggaacac aagcaatccg aaccaaaaat aattggggtc   2700

ccgtgcccac agagtctagt gcagacctaa aatgaccaca gtaaattata gctgttatta   2760

aacatgagat tttgaccaac aagagcgtag gaatgttatt agctactact tgtacataca   2820

cagcatttgt tttaaataat gttgcctcca ggggcagtga gatcaggacc cagatccgtg   2880

gccagctctc tgacttcaga ccgcttgtac ttaagcagct cgcaacactg ttgtcgagga   2940

ttgaacttgc catattcgat tttgtggtca tgaatccagc acacctc                 2987


<210>  28
<211>  14688
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZKLeuN-29E3

<400>  28
cgattgttgt ctactaacta tcgtacgata acttcgtata gcatacatta tacgaagtta     60

tcgcgtcgac gagtatctgt ctgactcgtc attgccgcct ttggagtacg actccaacta    120

tgagtgtgct tggatcactt tgacgataca ttcttcgttg gaggctgtgg gtctgacagc    180

tgcgttttcg gcgcggttgg ccgacaacaa tatcagctgc aacgtcattg ctggctttca    240

tcatgatcac atttttgtcg gcaaaggcga cgcccagaga gccattgacg ttctttctaa    300

tttggaccga tagccgtata gtccagtcta tctataagtt caactaactc gtaactatta    360

ccataacata tacttcactg ccccagataa ggttccgata aaaagttctg cagactaaat    420

ttatttcagt ctcctcttca ccaccaaaat gccctcctac gaagctcgag ctaacgtcca    480

caagtccgcc tttgccgctc gagtgctcaa gctcgtggca gccaagaaaa ccaacctgtg    540

tgcttctctg gatgttacca ccaccaagga gctcattgag cttgccgata aggtcggacc    600

ttatgtgtgc atgatcaaaa cccatatcga catcattgac gacttcacct acgccggcac    660

tgtgctcccc ctcaaggaac ttgctcttaa gcacggtttc ttcctgttcg aggacagaaa    720

gttcgcagat attggcaaca ctgtcaagca ccagtaccgg tgtcaccgaa tcgccgagtg    780

gtccgatatc accaacgccc acggtgtacc cggaaccgga atcattgctg gcctgcgagc    840

tggtgccgag gaaactgtct ctgaacagaa gaaggaggac gtctctgact acgagaactc    900

ccagtacaag gagttcctag tcccctctcc caacgagaag ctggccagag gtctgctcat    960

gctggccgag ctgtcttgca agggctctct ggccactggc gagtactcca agcagaccat   1020

tgagcttgcc cgatccgacc ccgagtttgt ggttggcttc attgcccaga accgacctaa   1080

gggcgactct gaggactggc ttattctgac ccccggggtg ggtcttgacg acaagggaga   1140

cgctctcgga cagcagtacc gaactgttga ggatgtcatg tctaccggaa cggatatcat   1200

aattgtcggc cgaggtctgt acggccagaa ccgagatcct attgaggagg ccaagcgata   1260

ccagaaggct ggctgggagg cttaccagaa gattaactgt tagaggttag actatggata   1320

tgtaatttaa ctgtgtatat agagagcgtg caagtatgga gcgcttgttc agcttgtatg   1380

atggtcagac gacctgtctg atcgagtatg tatgatactg cacaacctgt gtatccgcat   1440

gatctgtcca atggggcatg ttgttgtgtt tctcgatacg gagatgctgg gtacagtgct   1500

aatacgttga actacttata cttatatgag gctcgaagaa agctgacttg tgtatgactt   1560

attctcaact acatccccag tcacaatacc accactgcac taccactaca ccaaaaccat   1620

gatcaaacca cccatggact tcctggaggc agaagaactt gttatggaaa agctcaagag   1680

agagatcata acttcgtata gcatacatta tacgaagtta tcctgcaggt aaaggaattc   1740

tggagtttct gagagaaaaa ggcaagatac gtatgtaaca aagcgacgca tggtacaata   1800

ataccggagg catgtatcat agagagttag tggttcgatg atggcactgg tgcctggtat   1860

gactttatac ggctgactac atatttgtcc tcagacatac aattacagtc aagcacttac   1920

ccttggacat ctgtaggtac cccccggcca agacgatctc agcgtgtcgt atgtcggatt   1980

ggcgtagctc cctcgctcgt caattggctc ccatctactt tcttctgctt ggctacaccc   2040

agcatgtctg ctatggctcg ttttcgtgcc ttatctatcc tcccagtatt accaactcta   2100

aatgacatga tgtgattggg tctacacttt catatcagag ataaggagta gcacagttgc   2160

ataaaaagcc caactctaat cagcttcttc ctttcttgta attagtacaa aggtgattag   2220

cgaaatctgg aagcttagtt ggccctaaaa aaatcaaaaa aagcaaaaaa cgaaaaacga   2280

aaaaccacag ttttgagaac agggaggtaa cgaaggatcg tatatatata tatatatata   2340

tatacccacg gatcccgaga ccggcctttg attcttccct acaaccaacc attctcacca   2400

ccctaattca caaccatgga gtctggaccc atgcctgctg gcattccctt ccctgagtac   2460

tatgacttct ttatggactg gaagactccc ctggccatcg ctgccaccta cactgctgcc   2520

gtcggtctct tcaaccccaa ggttggcaag gtctcccgag tggttgccaa gtcggctaac   2580

gcaaagcctg ccgagcgaac ccagtccgga gctgccatga ctgccttcgt ctttgtgcac   2640

aacctcattc tgtgtgtcta ctctggcatc accttctact acatgtttcc tgctatggtc   2700

aagaacttcc gaacccacac actgcacgaa gcctactgcg acacggatca gtccctctgg   2760

aacaacgcac ttggctactg gggttacctc ttctacctgt ccaagttcta cgaggtcatt   2820

gacaccatca tcatcatcct gaagggacga cggtcctcgc tgcttcagac ctaccaccat   2880

gctggagcca tgattaccat gtggtctggc atcaactacc aagccactcc catttggatc   2940

tttgtggtct tcaactcctt cattcacacc atcatgtact gttactatgc cttcacctct   3000

atcggattcc atcctcctgg caaaaagtac ctgacttcga tgcagattac tcagtttctg   3060

gtcggtatca ccattgccgt gtcctacctc ttcgttcctg gctgcatccg aacacccggt   3120

gctcagatgg ctgtctggat caacgtcggc tacctgtttc ccttgaccta tctgttcgtg   3180

gactttgcca agcgaaccta ctccaagcga tctgccattg ccgctcagaa aaaggctcag   3240

taagcggccg cattgatgat tggaaacaca cacatgggtt atatctaggt gagagttagt   3300

tggacagtta tatattaaat cagctatgcc aacggtaact tcattcatgt caacgaggaa   3360

ccagtgactg caagtaatat agaatttgac caccttgcca ttctcttgca ctcctttact   3420

atatctcatt tatttcttat atacaaatca cttcttcttc ccagcatcga gctcggaaac   3480

ctcatgagca ataacatcgt ggatctcgtc aatagagggc tttttggact ccttgctgtt   3540

ggccaccttg tccttgctgt ctggctcatt ctgtttcaac gccttttaat taacggagta   3600

ggtctcggtg tcggaagcga cgccagatcc gtcatcctcc tttcgctctc caaagtagat   3660

acctccgacg agctctcgga caatgatgaa gtcggtgccc tcaacgtttc ggatggggga   3720

gagatcggcg agcttgggcg acagcagctg gcagggtcgc aggttggcgt acaggttcag   3780

gtcctttcgc agcttgagga gaccctgctc gggtcgcacg tcggttcgtc cgtcgggagt   3840

ggtccatacg gtgttggcag cgcctccgac agcaccgagc ataatagagt cagcctttcg   3900

gcagatgtcg agagtagcgt cggtgatggg ctcgccctcc ttctcaatgg cagctcctcc   3960

aatgagtcgg tcctcaaaca caaactcggt gccggaggcc tcagcaacag acttgagcac   4020

cttgacggcc tcggcaatca cctcggggcc acagaagtcg ccgccgagaa gaacaatctt   4080

cttggagtca gtcttggtct tcttagtttc gggttccatt gtggatgtgt gtggttgtat   4140

gtgtgatgtg gtgtgtggag tgaaaatctg tggctggcaa acgctcttgt atatatacgc   4200

acttttgccc gtgctatgtg gaagactaaa cctccgaaga ttgtgactca ggtagtgcgg   4260

tatcggctag ggacccaaac cttgtcgatg ccgatagcat gcgacgtcgg gcccaattcg   4320

ccctatagtg agtcgtatta caattcactg gccgtcgttt tacaacgtcg tgactgggaa   4380

aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt   4440

aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa   4500

tggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga   4560

ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg   4620

ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat   4680

ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg   4740

ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata   4800

gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt   4860

tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat   4920

ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc ctgatgcggt attttctcct   4980

tacgcatctg tgcggtattt cacaccgcat caggtggcac ttttcgggga aatgtgcgcg   5040

gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat   5100

aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc   5160

gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa   5220

cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac   5280

tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga   5340

tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag   5400

agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca   5460

cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca   5520

tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa   5580

ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc   5640

tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa   5700

cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag   5760

actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct   5820

ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac   5880

tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa   5940

ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt   6000

aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat   6060

ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg   6120

agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc   6180

ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg   6240

tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag   6300

cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact   6360

ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg   6420

gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc   6480

ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg   6540

aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg   6600

cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag   6660

ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc   6720

gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct   6780

ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc   6840

ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc   6900

gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac   6960

cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcgcgcccac tgagctcgtc   7020

taacggactt gatatacaac caattaaaac aaatgaaaag aaatacagtt ctttgtatca   7080

tttgtaacaa ttaccctgta caaactaagg tattgaaatc ccacaatatt cccaaagtcc   7140

acccctttcc aaattgtcat gcctacaact catataccaa gcactaacct accaaacacc   7200

actaaaaccc cacaaaatat atcttaccga atatacagta acaagctacc accacactcg   7260

ttgggtgcag tcgccagctt aaagatatct atccacatca gccacaactc ccttccttta   7320

ataaaccgac tacacccttg gctattgagg ttatgagtga atatactgta gacaagacac   7380

tttcaagaag actgtttcca aaacgtacca ctgtcctcca ctacaaacac acccaatctg   7440

cttcttctag tcaaggttgc tacaccggta aattataaat catcatttca ttagcagggc   7500

agggcccttt ttatagagtc ttatacacta gcggaccctg ccggtagacc aacccgcagg   7560

cgcgtcagtt tgctccttcc atcaatgcgt cgtagaaacg acttactcct tcttgagcag   7620

ctccttgacc ttgttggcaa caagtctccg acctcggagg tggaggaaga gcctccgata   7680

tcggcggtag tgataccagc ctcgacggac tccttgacgg cagcctcaac agcgtcaccg   7740

gcgggcttca tgttaagaga gaacttgagc atcatggcgg cagacagaat ggtggcgtac   7800

gcaactaaca tgaatgaata cgatatacat caaagactat gatacgcagt attgcacact   7860

gtacgagtaa gagcactagc cactgcactc aagtgaaacc gttgcccggg tacgagtatg   7920

agtatgtaca gtatgtttag tattgtactt ggacagtgct tgtatcgtac attctcaagt   7980

gtcaaacata aatatccgtt gctatatcct cgcaccacca cgtagctcgc tatatccctg   8040

tgttgaatcc atccatcttg gattgccaat tgtgcacaca gaaccgggca ctcacttccc   8100

catccacact tgcggccgct taagcaacgg gcttgataac agcggggggg gtgcccacgt   8160

tgttgcggtt gcggaagaac agaacaccct taccagcacc ctcggcacca gcgctgggct   8220

caacccactg gcacatacgc gcactgcggt acatggcgcg gatgaagcca cgaggaccat   8280

cctggacatc agcccggtag tgcttgccca tgatgggctt aatggcctcg gtggcctcgt   8340

ccgcgttgta gaaggggatg ctgctgacgt agtggtggag gacatgagtc tcgatgatgc   8400

cgtggagaag gtggcggccg atgaagccca tctcacggtc aatggtagca gcggcaccac   8460

ggacgaagtt ccactcgtcg ttggtgtagt ggggaagggt agggtcggtg tgctggagga   8520

aggtgatggc aacgagccag tggttaaccc agaggtaggg aacaaagtac cagatggcca   8580

tgttgtagaa accgaacttc tgaacgagga agtacagagc agtggccatc agaccgatac   8640

caatatcgct gaggacgatg agcttagcgt cactgttctc gtacagaggg ctgcggggat   8700

cgaagtggtt aacaccaccg ccgaggccgt tatgcttgcc cttgccgcga ccctcacgct   8760

ggcgctcgtg gtagttgtgg ccggtaacat tggtgatgag gtagttgggc cagccaacga   8820

gctgctgaag gacgagcatg agaagagtga aagcgggggt ctcctcagta agatgagcga   8880

gctcgtgggt catctttccg agacgagtag cctgctgctc gcgggttcgg ggaacgaaga   8940

ccatgtcacg ctccatgttg ccagtggcct tgtggtgctt tcggtgggag atttgccagc   9000

tgaagtaggg gacaaggagg gaagagtgaa gaacccagcc agtaatgtcg ttgatgatgc   9060

gagaatcgga gaaagcaccg tgaccgcact catgggcaat aacccagaga ccagtaccga   9120

aaagaccctg aagaacggtg tacacggccc acagaccagc gcgggcgggg gtggagggga   9180

tatattcggg ggtcacaaag ttgtaccaga tgctgaaagt ggtagtcagg aggacaatgt   9240

cgcggaggat ataaccgtat cccttgagag cggagcgctt gaagcagtgc ttagggatgg   9300

cattgtagat gtccttgatg gtaaagtcgg gaacctcgaa ctggttgccg taggtgtcga   9360

gcatgacacc atactcggac ttgggcttgg cgatatcaac ctcggacatg gacgagagcg   9420

atgtggaaga ggccgagtgg cggggagagt ctgaaggaga gacggcggca gactcagaat   9480

ccgtcacagt agttgaggtg acggtgcgtc taagcgcagg gttctgcttg ggcagagccg   9540

aagtggacgc catggttgat gtgtgtttaa ttcaagaatg aatatagaga agagaagaag   9600

aaaaaagatt caattgagcc ggcgatgcag acccttatat aaatgttgcc ttggacagac   9660

ggagcaagcc cgcccaaacc tacgttcggt ataatatgtt aagcttttta acacaaaggt   9720

ttggcttggg gtaacctgat gtggtgcaaa agaccgggcg ttggcgagcc attgcgcggg   9780

cgaatggggc cgtgactcgt ctcaaattcg agggcgtgcc tcaattcgtg cccccgtggc   9840

tttttcccgc cgtttccgcc ccgtttgcac cactgcagcc gcttctttgg ttcggacacc   9900

ttgctgcgag ctaggtgcct tgtgctactt aaaaagtggc ctcccaacac caacatgaca   9960

tgagtgcgtg ggccaagaca cgttggcggg gtcgcagtcg gctcaatggc ccggaaaaaa  10020

cgctgctgga gctggttcgg acgcagtccg ccgcggcgta tggatatccg caaggttcca  10080

tagcgccatt gccctccgtc ggcgtctatc ccgcaacctc taaatagagc gggaatataa  10140

cccaagcttc ttttttttcc tttaacacgc acacccccaa ctatcatgtt gctgctgctg  10200

tttgactcta ctctgtggag gggtgctccc acccaaccca acctacaggt ggatccggcg  10260

ctgtgattgg ctgataagtc tcctatccgg actaattctg accaatggga catgcgcgca  10320

ggacccaaat gccgcaatta cgtaacccca acgaaatgcc tacccctctt tggagcccag  10380

cggccccaaa tccccccaag cagcccggtt ctaccggctt ccatctccaa gcacaagcag  10440

cccggttcta ccggcttcca tctccaagca cccctttctc cacaccccac aaaaagaccc  10500

gtgcaggaca tcctactgcg tcgacatcat ttaaattcct tcacttcaag ttcattcttc  10560

atctgcttct gttttacttt gacaggcaaa tgaagacatg gtacgacttg atggaggcca  10620

agaacgccat ttcaccccga gacaccgaag tgcctgaaat cctggctgcc cccattgata  10680

acatcggaaa ctacggtatt ccggaaagtg tatatagaac ctttccccag cttgtgtctg  10740

tggatatgga tggtgtaatc ccctttgagt actcgtcttg gcttctctcc gagcagtatg  10800

aggctctcta atctagcgca tttaatatct caatgtattt atatatttat cttctcatgc  10860

ggccgctcac tgaatctttt tggctccctt gtgcttcctg acgatatacg tttgcacata  10920

gaaattcaag aacaaacaca agactgtgcc aacataaaag taattgaaga accagccaaa  10980

catcctcatc ccatcttggc gataacaggg aatgttcctg tacttccaga caatgtagaa  11040

accaacattg aattgaatga tctgcattga tgtaatcagg gattttggca tggggaactt  11100

cagcttgatc aatctggtcc aataataacc gtacatgatc cagtggatga aaccattcaa  11160

cagcacaaaa atccaaacag cttcatttcg gtaattatag aacagccaca tatccatcgg  11220

tgcccccaaa tgatggaaga attgcaacca ggtcagaggc ttgcccatca gtggcaaata  11280

gaaggagtca atatactcca ggaacttgct caaatagaac aactgcgtgg tgatcctgaa  11340

gacgttgttg tcaaaagcct tctcgcagtt gtcagacata acaccgatgg tgtacatggc  11400

atatgccatt gagaggaatg atcccaacga ataaatggac atgagaaggt tgtaattggt  11460

gaaaacaaac ttcatacgag actgaccttt tggaccaagg gggccaagag tgaacttcaa  11520

gatgacaaat gcgatggaca agtaaagcac ctcacagtga ctggcatcac tccagagttg  11580

ggcataatca actggttggg taaaacttcc tgcccaattg agactatttc attcaccacc  11640

tccatggcca ttgctgtaga tatgtcttgt gtgtaagggg gttggggtgg ttgtttgtgt  11700

tcttgacttt tgtgttagca agggaagacg ggcaaaaaag tgagtgtggt tgggagggag  11760

agacgagcct tatatataat gcttgtttgt gtttgtgcaa gtggacgccg aaacgggcag  11820

gagccaaact aaacaaggca gacaatgcga gcttaattgg attgcctgat gggcaggggt  11880

tagggctcga tcaatggggg tgcgaagtga caaaattggg aattaggttc gcaagcaagg  11940

ctgacaagac tttggcccaa acatttgtac gcggtggaca acaggagcca cccatcgtct  12000

gtcacgggct agccggtcgt gcgtcctgtc aggctccacc taggctccat gccactccat  12060

acaatcccac tagtgtaccg ctaggccgct tttagctccc atctaagacc cccccaaaac  12120

ctccactgta cagtgcactg tactgtgtgg cgatcaaggg caagggaaaa aaggcgcaaa  12180

catgcacgca tggaatgacg taggtaaggc gttactagac tgaaaagtgg cacatttcgg  12240

cgtgccaaag ggtcctaggt gcgtttcgcg agctgggcgc caggccaagc cgctccaaaa  12300

cgcctctccg actccctcca gcggcctcca tatccccatc cctctccaca gcaatgttgt  12360

taagccttgc aaacgaaaaa atagaaaggc taataagctt ccaatattgt ggtgtacgct  12420

gcataacgca acaatgagcg ccaaacaaca cacacacaca gcacacagca gcattaacca  12480

cgatgaacag catgacatta caggtgggtg tgtaatcagg gccctgattg ctggtggtgg  12540

gagcccccat catgggcaga tctgcgtaca ctgtttaaac agtgtacgca gatctactat  12600

agaggaacat ttaaattgcc ccggagaaga cggccaggcc gcctagatga caaattcaac  12660

aactcacagc tgactttctg ccattgccac tagggggggg cctttttata tggccaagcc  12720

aagctctcca cgtcggttgg gctgcaccca acaataaatg ggtagggttg caccaacaaa  12780

gggatgggat ggggggtaga agatacgagg ataacggggc tcaatggcac aaataagaac  12840

gaatactgcc attaagactc gtgatccagc gactgacacc attgcatcat ctaagggcct  12900

caaaactacc tcggaactgc tgcgctgatc tggacaccac agaggttccg agcactttag  12960

gttgcaccaa atgtcccacc aggtgcaggc agaaaacgct ggaacagcgt gtacagtttg  13020

tcttaacaaa aagtgagggc gctgaggtcg agcagggtgg tgtgacttgt tatagccttt  13080

agagctgcga aagcgcgtat ggatttggct catcaggcca gattgagggt ctgtggacac  13140

atgtcatgtt agtgtacttc aatcgccccc tggatatagc cccgacaata ggccgtggcc  13200

tcattttttt gccttccgca catttccatt gctcgatacc cacaccttgc ttctcctgca  13260

cttgccaacc ttaatactgg tttacattga ccaacatctt acaagcgggg ggcttgtcta  13320

gggtatatat aaacagtggc tctcccaatc ggttgccagt ctcttttttc ctttctttcc  13380

ccacagattc gaaatctaaa ctacacatca cagaattccg agccgtgagt atccacgaca  13440

agatcagtgt cgagacgacg cgttttgtgt aatgacacaa tccgaaagtc gctagcaaca  13500

cacactctct acacaaacta acccagctct ggtaccatgg aggtcgtgaa cgaaatcgtc  13560

tccattggcc aggaggttct tcccaaggtc gactatgctc agctctggtc tgatgcctcg  13620

cactgcgagg tgctgtacct ctccatcgcc ttcgtcatcc tgaagttcac ccttggtcct  13680

ctcggaccca agggtcagtc tcgaatgaag tttgtgttca ccaactacaa cctgctcatg  13740

tccatctact cgctgggctc cttcctctct atggcctacg ccatgtacac cattggtgtc  13800

atgtccgaca actgcgagaa ggctttcgac aacaatgtct tccgaatcac cactcagctg  13860

ttctacctca gcaagttcct cgagtacatt gactccttct atctgcccct catgggcaag  13920

cctctgacct ggttgcagtt ctttcaccat ctcggagctc ctatggacat gtggctgttc  13980

tacaactacc gaaacgaagc cgtttggatc tttgtgctgc tcaacggctt cattcactgg  14040

atcatgtacg gctactattg gacccgactg atcaagctca agttccctat gcccaagtcc  14100

ctgattactt ctatgcagat cattcagttc aacgttggct tctacatcgt ctggaagtac  14160

cggaacattc cctgctaccg acaagatgga atgagaatgt ttggctggtt tttcaactac  14220

ttctacgttg gtactgtcct gtgtctgttc ctcaacttct acgtgcagac ctacatcgtc  14280

cgaaagcaca agggagccaa aaagattcag tgagcggccg catgtacata caagattatt  14340

tatagaaatg aatcgcgatc gaacaaagag tacgagtgta cgagtagggg atgatgataa  14400

aagtggaaga agttccgcat ctttggattt atcaacgtgt aggacgatac ttcctgtaaa  14460

aatgcaatgt ctttaccata ggttctgctg tagatgttat taactaccat taacatgtct  14520

acttgtacag ttgcagacca gttggagtat agaatggtac acttaccaaa aagtgttgat  14580

ggttgtaact acgatatata aaactgttga cgggatcccc gctgatatgc ctaaggaaca  14640

atcaaagagg aagatattaa ttcagaatgc tagtatacag ttagggat               14688


<210>  29
<211>  1434
<212>  DNA
<213>  Fusarium monoliforme


<220>
<221>  CDS
<222>  (1)..(1434)
<223>  delta-12 desaturase

<300>
<302>  DELTA-12 DESATURASES SUITABLE FOR ALTERING LEVELS OF 
       POLYUNSATURATED FATTY ACIDS IN OLEAGINOUS YEAST
<310>  WO 2005/047485
<311>  2004-11-12
<312>  2005-05-26
<313>  (1)..(1434)

<300>
<302>  DELTA-12 DESATURASES SUITABLE FOR ALTERING LEVELS OF 
       POLYUNSATURATED FATTY ACIDS IN OLEAGINOUS YEAST
<310>  US 2005-0216975-A1
<311>  2004-11-10
<312>  2005-09-29
<313>  (1)..(1434)

<400>  29
atg gcg tcc act tcg gct ctg ccc aag cag aac cct gcg ctt aga cgc       48
Met Ala Ser Thr Ser Ala Leu Pro Lys Gln Asn Pro Ala Leu Arg Arg         
1               5                   10                  15              

acc gtc acc tca act act gtg acg gat tct gag tct gcc gcc gtc tct       96
Thr Val Thr Ser Thr Thr Val Thr Asp Ser Glu Ser Ala Ala Val Ser         
            20                  25                  30                  

cct tca gac tct ccc cgc cac tcg gcc tct tcc aca tcg ctc tcg tcc      144
Pro Ser Asp Ser Pro Arg His Ser Ala Ser Ser Thr Ser Leu Ser Ser         
        35                  40                  45                      

atg tcc gag gtt gat atc gcc aag ccc aag tcc gag tat ggt gtc atg      192
Met Ser Glu Val Asp Ile Ala Lys Pro Lys Ser Glu Tyr Gly Val Met         
    50                  55                  60                          

ctc gac acc tac ggc aac cag ttc gag gtt ccc gac ttt acc atc aag      240
Leu Asp Thr Tyr Gly Asn Gln Phe Glu Val Pro Asp Phe Thr Ile Lys         
65                  70                  75                  80          

gac atc tac aat gcc atc cct aag cac tgc ttc aag cgc tcc gct ctc      288
Asp Ile Tyr Asn Ala Ile Pro Lys His Cys Phe Lys Arg Ser Ala Leu         
                85                  90                  95              

aag gga tac ggt tat atc ctc cgc gac att gtc ctc ctg act acc act      336
Lys Gly Tyr Gly Tyr Ile Leu Arg Asp Ile Val Leu Leu Thr Thr Thr         
            100                 105                 110                 

ttc agc atc tgg tac aac ttt gtg acc ccc gaa tat atc ccc tcc acc      384
Phe Ser Ile Trp Tyr Asn Phe Val Thr Pro Glu Tyr Ile Pro Ser Thr         
        115                 120                 125                     

ccc gcc cgc gct ggt ctg tgg gcc gtg tac acc gtt ctt cag ggt ctt      432
Pro Ala Arg Ala Gly Leu Trp Ala Val Tyr Thr Val Leu Gln Gly Leu         
    130                 135                 140                         

ttc ggt act ggt ctc tgg gtt att gcc cat gag tgc ggt cac ggt gct      480
Phe Gly Thr Gly Leu Trp Val Ile Ala His Glu Cys Gly His Gly Ala         
145                 150                 155                 160         

ttc tcc gat tct cgc atc atc aac gac att act ggc tgg gtt ctt cac      528
Phe Ser Asp Ser Arg Ile Ile Asn Asp Ile Thr Gly Trp Val Leu His         
                165                 170                 175             

tct tcc ctc ctt gtc ccc tac ttc agc tgg caa atc tcc cac cga aag      576
Ser Ser Leu Leu Val Pro Tyr Phe Ser Trp Gln Ile Ser His Arg Lys         
            180                 185                 190                 

cac cac aag gcc act ggc aac atg gag cgt gac atg gtc ttc gtt ccc      624
His His Lys Ala Thr Gly Asn Met Glu Arg Asp Met Val Phe Val Pro         
        195                 200                 205                     

cga acc cgc gag cag cag gct act cgt ctc gga aag atg acc cac gag      672
Arg Thr Arg Glu Gln Gln Ala Thr Arg Leu Gly Lys Met Thr His Glu         
    210                 215                 220                         

ctc gct cat ctt act gag gag acc ccc gct ttc act ctt ctc atg ctc      720
Leu Ala His Leu Thr Glu Glu Thr Pro Ala Phe Thr Leu Leu Met Leu         
225                 230                 235                 240         

gtc ctt cag cag ctc gtt ggc tgg ccc aac tac ctc atc acc aat gtt      768
Val Leu Gln Gln Leu Val Gly Trp Pro Asn Tyr Leu Ile Thr Asn Val         
                245                 250                 255             

acc ggc cac aac tac cac gag cgc cag cgt gag ggt cgc ggc aag ggc      816
Thr Gly His Asn Tyr His Glu Arg Gln Arg Glu Gly Arg Gly Lys Gly         
            260                 265                 270                 

aag cat aac ggc ctc ggc ggt ggt gtt aac cac ttc gat ccc cgc agc      864
Lys His Asn Gly Leu Gly Gly Gly Val Asn His Phe Asp Pro Arg Ser         
        275                 280                 285                     

cct ctg tac gag aac agt gac gct aag ctc atc gtc ctc agc gat att      912
Pro Leu Tyr Glu Asn Ser Asp Ala Lys Leu Ile Val Leu Ser Asp Ile         
    290                 295                 300                         

ggt atc ggt ctg atg gcc act gct ctg tac ttc ctc gtt cag aag ttc      960
Gly Ile Gly Leu Met Ala Thr Ala Leu Tyr Phe Leu Val Gln Lys Phe         
305                 310                 315                 320         

ggt ttc tac aac atg gcc atc tgg tac ttt gtt ccc tac ctc tgg gtt     1008
Gly Phe Tyr Asn Met Ala Ile Trp Tyr Phe Val Pro Tyr Leu Trp Val         
                325                 330                 335             

aac cac tgg ctc gtt gcc atc acc ttc ctc cag cac acc gac cct acc     1056
Asn His Trp Leu Val Ala Ile Thr Phe Leu Gln His Thr Asp Pro Thr         
            340                 345                 350                 

ctt ccc cac tac acc aac gac gag tgg aac ttc gtc cgt ggt gcc gct     1104
Leu Pro His Tyr Thr Asn Asp Glu Trp Asn Phe Val Arg Gly Ala Ala         
        355                 360                 365                     

gct acc att gac cgt gag atg ggc ttc atc ggc cgc cac ctt ctc cac     1152
Ala Thr Ile Asp Arg Glu Met Gly Phe Ile Gly Arg His Leu Leu His         
    370                 375                 380                         

ggc atc atc gag act cat gtc ctc cac cac tac gtc agc agc atc ccc     1200
Gly Ile Ile Glu Thr His Val Leu His His Tyr Val Ser Ser Ile Pro         
385                 390                 395                 400         

ttc tac aac gcg gac gag gcc acc gag gcc att aag ccc atc atg ggc     1248
Phe Tyr Asn Ala Asp Glu Ala Thr Glu Ala Ile Lys Pro Ile Met Gly         
                405                 410                 415             

aag cac tac cgg gct gat gtc cag gat ggt cct cgt ggc ttc atc cgc     1296
Lys His Tyr Arg Ala Asp Val Gln Asp Gly Pro Arg Gly Phe Ile Arg         
            420                 425                 430                 

gcc atg tac cgc agt gcg cgt atg tgc cag tgg gtt gag ccc agc gct     1344
Ala Met Tyr Arg Ser Ala Arg Met Cys Gln Trp Val Glu Pro Ser Ala         
        435                 440                 445                     

ggt gcc gag ggt gct ggt aag ggt gtt ctg ttc ttc cgc aac cgc aac     1392
Gly Ala Glu Gly Ala Gly Lys Gly Val Leu Phe Phe Arg Asn Arg Asn         
    450                 455                 460                         

aac gtg ggc acc ccc ccc gct gtt atc aag ccc gtt gct taa             1434
Asn Val Gly Thr Pro Pro Ala Val Ile Lys Pro Val Ala                     
465                 470                 475                             


<210>  30
<211>  477
<212>  PRT
<213>  Fusarium monoliforme

<400>  30

Met Ala Ser Thr Ser Ala Leu Pro Lys Gln Asn Pro Ala Leu Arg Arg 
1               5                   10                  15      


Thr Val Thr Ser Thr Thr Val Thr Asp Ser Glu Ser Ala Ala Val Ser 
            20                  25                  30          


Pro Ser Asp Ser Pro Arg His Ser Ala Ser Ser Thr Ser Leu Ser Ser 
        35                  40                  45              


Met Ser Glu Val Asp Ile Ala Lys Pro Lys Ser Glu Tyr Gly Val Met 
    50                  55                  60                  


Leu Asp Thr Tyr Gly Asn Gln Phe Glu Val Pro Asp Phe Thr Ile Lys 
65                  70                  75                  80  


Asp Ile Tyr Asn Ala Ile Pro Lys His Cys Phe Lys Arg Ser Ala Leu 
                85                  90                  95      


Lys Gly Tyr Gly Tyr Ile Leu Arg Asp Ile Val Leu Leu Thr Thr Thr 
            100                 105                 110         


Phe Ser Ile Trp Tyr Asn Phe Val Thr Pro Glu Tyr Ile Pro Ser Thr 
        115                 120                 125             


Pro Ala Arg Ala Gly Leu Trp Ala Val Tyr Thr Val Leu Gln Gly Leu 
    130                 135                 140                 


Phe Gly Thr Gly Leu Trp Val Ile Ala His Glu Cys Gly His Gly Ala 
145                 150                 155                 160 


Phe Ser Asp Ser Arg Ile Ile Asn Asp Ile Thr Gly Trp Val Leu His 
                165                 170                 175     


Ser Ser Leu Leu Val Pro Tyr Phe Ser Trp Gln Ile Ser His Arg Lys 
            180                 185                 190         


His His Lys Ala Thr Gly Asn Met Glu Arg Asp Met Val Phe Val Pro 
        195                 200                 205             


Arg Thr Arg Glu Gln Gln Ala Thr Arg Leu Gly Lys Met Thr His Glu 
    210                 215                 220                 


Leu Ala His Leu Thr Glu Glu Thr Pro Ala Phe Thr Leu Leu Met Leu 
225                 230                 235                 240 


Val Leu Gln Gln Leu Val Gly Trp Pro Asn Tyr Leu Ile Thr Asn Val 
                245                 250                 255     


Thr Gly His Asn Tyr His Glu Arg Gln Arg Glu Gly Arg Gly Lys Gly 
            260                 265                 270         


Lys His Asn Gly Leu Gly Gly Gly Val Asn His Phe Asp Pro Arg Ser 
        275                 280                 285             


Pro Leu Tyr Glu Asn Ser Asp Ala Lys Leu Ile Val Leu Ser Asp Ile 
    290                 295                 300                 


Gly Ile Gly Leu Met Ala Thr Ala Leu Tyr Phe Leu Val Gln Lys Phe 
305                 310                 315                 320 


Gly Phe Tyr Asn Met Ala Ile Trp Tyr Phe Val Pro Tyr Leu Trp Val 
                325                 330                 335     


Asn His Trp Leu Val Ala Ile Thr Phe Leu Gln His Thr Asp Pro Thr 
            340                 345                 350         


Leu Pro His Tyr Thr Asn Asp Glu Trp Asn Phe Val Arg Gly Ala Ala 
        355                 360                 365             


Ala Thr Ile Asp Arg Glu Met Gly Phe Ile Gly Arg His Leu Leu His 
    370                 375                 380                 


Gly Ile Ile Glu Thr His Val Leu His His Tyr Val Ser Ser Ile Pro 
385                 390                 395                 400 


Phe Tyr Asn Ala Asp Glu Ala Thr Glu Ala Ile Lys Pro Ile Met Gly 
                405                 410                 415     


Lys His Tyr Arg Ala Asp Val Gln Asp Gly Pro Arg Gly Phe Ile Arg 
            420                 425                 430         


Ala Met Tyr Arg Ser Ala Arg Met Cys Gln Trp Val Glu Pro Ser Ala 
        435                 440                 445             


Gly Ala Glu Gly Ala Gly Lys Gly Val Leu Phe Phe Arg Asn Arg Asn 
    450                 455                 460                 


Asn Val Gly Thr Pro Pro Ala Val Ile Lys Pro Val Ala 
465                 470                 475         


<210>  31
<211>  777
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(777)
<223>  synthetic delta-9 elongase (codon-optimized for Yarrowia 
       lipolytica)

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  WO 2007/061742
<311>  2006-11-16
<312>  2007-05-31
<313>  (1)..(777)

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  US-2007-0117190-A1
<311>  2006-11-16
<312>  2007-05-24
<313>  (1)..(777)

<400>  31
atg gag gtc gtg aac gaa atc gtc tcc att ggc cag gag gtt ctt ccc       48
Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro         
1               5                   10                  15              

aag gtc gac tat gct cag ctc tgg tct gat gcc tcg cac tgc gag gtg       96
Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val         
            20                  25                  30                  

ctg tac ctc tcc atc gcc ttc gtc atc ctg aag ttc acc ctt ggt cct      144
Leu Tyr Leu Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro         
        35                  40                  45                      

ctc gga ccc aag ggt cag tct cga atg aag ttt gtg ttc acc aac tac      192
Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr         
    50                  55                  60                          

aac ctg ctc atg tcc atc tac tcg ctg ggc tcc ttc ctc tct atg gcc      240
Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala         
65                  70                  75                  80          

tac gcc atg tac acc att ggt gtc atg tcc gac aac tgc gag aag gct      288
Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala         
                85                  90                  95              

ttc gac aac aat gtc ttc cga atc acc act cag ctg ttc tac ctc agc      336
Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser         
            100                 105                 110                 

aag ttc ctc gag tac att gac tcc ttc tat ctg ccc ctc atg ggc aag      384
Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys         
        115                 120                 125                     

cct ctg acc tgg ttg cag ttc ttt cac cat ctc gga gct cct atg gac      432
Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp         
    130                 135                 140                         

atg tgg ctg ttc tac aac tac cga aac gaa gcc gtt tgg atc ttt gtg      480
Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val         
145                 150                 155                 160         

ctg ctc aac ggc ttc att cac tgg atc atg tac ggc tac tat tgg acc      528
Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr         
                165                 170                 175             

cga ctg atc aag ctc aag ttc cct atg ccc aag tcc ctg att act tct      576
Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser         
            180                 185                 190                 

atg cag atc att cag ttc aac gtt ggc ttc tac atc gtc tgg aag tac      624
Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr         
        195                 200                 205                     

cgg aac att ccc tgc tac cga caa gat gga atg aga atg ttt ggc tgg      672
Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp         
    210                 215                 220                         

ttt ttc aac tac ttc tac gtt ggt act gtc ctg tgt ctg ttc ctc aac      720
Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn         
225                 230                 235                 240         

ttc tac gtg cag acc tac atc gtc cga aag cac aag gga gcc aaa aag      768
Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys         
                245                 250                 255             

att cag tga                                                          777
Ile Gln                                                                 
                                                                        


<210>  32
<211>  258
<212>  PRT
<213>  Euglena gracilis

<400>  32

Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro 
1               5                   10                  15      


Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val 
            20                  25                  30          


Leu Tyr Leu Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro 
        35                  40                  45              


Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr 
    50                  55                  60                  


Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala 
65                  70                  75                  80  


Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala 
                85                  90                  95      


Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser 
            100                 105                 110         


Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys 
        115                 120                 125             


Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp 
    130                 135                 140                 


Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val 
145                 150                 155                 160 


Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr 
                165                 170                 175     


Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 
        195                 200                 205             


Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp 
    210                 215                 220                 


Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys 
                245                 250                 255     


Ile Gln 
        


<210>  33
<211>  34
<212>  DNA
<213>  Escherichia coli

<400>  33
ataacttcgt ataatgtatg ctatacgaag ttat                                 34


<210>  34
<211>  828
<212>  DNA
<213>  Mortierella alpina


<220>
<221>  CDS
<222>  (1)..(828)
<223>  synthetic C16/18 elongase (codon-optimized for Yarrowia 
       lipolytica)

<300>
<302>  A MORTIERELLA ALPINA C16/18 FATTY ACID ELONGASE
<310>  US 2007-0087420-A1
<311>  2005-10-19
<312>  2007-04-19
<313>  (1)..(828)

<300>
<302>  A MORTIERELLA ALPINA C16/18 FATTY ACID ELONGASE
<310>  WO 2007/046817
<311>  2005-11-04
<312>  2007-04-26
<313>  (1)..(828)

<400>  34
atg gag tct gga ccc atg cct gct ggc att ccc ttc cct gag tac tat       48
Met Glu Ser Gly Pro Met Pro Ala Gly Ile Pro Phe Pro Glu Tyr Tyr         
1               5                   10                  15              

gac ttc ttt atg gac tgg aag act ccc ctg gcc atc gct gcc acc tac       96
Asp Phe Phe Met Asp Trp Lys Thr Pro Leu Ala Ile Ala Ala Thr Tyr         
            20                  25                  30                  

act gct gcc gtc ggt ctc ttc aac ccc aag gtt ggc aag gtc tcc cga      144
Thr Ala Ala Val Gly Leu Phe Asn Pro Lys Val Gly Lys Val Ser Arg         
        35                  40                  45                      

gtg gtt gcc aag tcg gct aac gca aag cct gcc gag cga acc cag tcc      192
Val Val Ala Lys Ser Ala Asn Ala Lys Pro Ala Glu Arg Thr Gln Ser         
    50                  55                  60                          

gga gct gcc atg act gcc ttc gtc ttt gtg cac aac ctc att ctg tgt      240
Gly Ala Ala Met Thr Ala Phe Val Phe Val His Asn Leu Ile Leu Cys         
65                  70                  75                  80          

gtc tac tct ggc atc acc ttc tac tac atg ttt cct gct atg gtc aag      288
Val Tyr Ser Gly Ile Thr Phe Tyr Tyr Met Phe Pro Ala Met Val Lys         
                85                  90                  95              

aac ttc cga acc cac aca ctg cac gaa gcc tac tgc gac acg gat cag      336
Asn Phe Arg Thr His Thr Leu His Glu Ala Tyr Cys Asp Thr Asp Gln         
            100                 105                 110                 

tcc ctc tgg aac aac gca ctt ggc tac tgg ggt tac ctc ttc tac ctg      384
Ser Leu Trp Asn Asn Ala Leu Gly Tyr Trp Gly Tyr Leu Phe Tyr Leu         
        115                 120                 125                     

tcc aag ttc tac gag gtc att gac acc atc atc atc atc ctg aag gga      432
Ser Lys Phe Tyr Glu Val Ile Asp Thr Ile Ile Ile Ile Leu Lys Gly         
    130                 135                 140                         

cga cgg tcc tcg ctg ctt cag acc tac cac cat gct gga gcc atg att      480
Arg Arg Ser Ser Leu Leu Gln Thr Tyr His His Ala Gly Ala Met Ile         
145                 150                 155                 160         

acc atg tgg tct ggc atc aac tac caa gcc act ccc att tgg atc ttt      528
Thr Met Trp Ser Gly Ile Asn Tyr Gln Ala Thr Pro Ile Trp Ile Phe         
                165                 170                 175             

gtg gtc ttc aac tcc ttc att cac acc atc atg tac tgt tac tat gcc      576
Val Val Phe Asn Ser Phe Ile His Thr Ile Met Tyr Cys Tyr Tyr Ala         
            180                 185                 190                 

ttc acc tct atc gga ttc cat cct cct ggc aaa aag tac ctg act tcg      624
Phe Thr Ser Ile Gly Phe His Pro Pro Gly Lys Lys Tyr Leu Thr Ser         
        195                 200                 205                     

atg cag att act cag ttt ctg gtc ggt atc acc att gcc gtg tcc tac      672
Met Gln Ile Thr Gln Phe Leu Val Gly Ile Thr Ile Ala Val Ser Tyr         
    210                 215                 220                         

ctc ttc gtt cct ggc tgc atc cga aca ccc ggt gct cag atg gct gtc      720
Leu Phe Val Pro Gly Cys Ile Arg Thr Pro Gly Ala Gln Met Ala Val         
225                 230                 235                 240         

tgg atc aac gtc ggc tac ctg ttt ccc ttg acc tat ctg ttc gtg gac      768
Trp Ile Asn Val Gly Tyr Leu Phe Pro Leu Thr Tyr Leu Phe Val Asp         
                245                 250                 255             

ttt gcc aag cga acc tac tcc aag cga tct gcc att gcc gct cag aaa      816
Phe Ala Lys Arg Thr Tyr Ser Lys Arg Ser Ala Ile Ala Ala Gln Lys         
            260                 265                 270                 

aag gct cag taa                                                      828
Lys Ala Gln                                                             
        275                                                             


<210>  35
<211>  275
<212>  PRT
<213>  Mortierella alpina

<400>  35

Met Glu Ser Gly Pro Met Pro Ala Gly Ile Pro Phe Pro Glu Tyr Tyr 
1               5                   10                  15      


Asp Phe Phe Met Asp Trp Lys Thr Pro Leu Ala Ile Ala Ala Thr Tyr 
            20                  25                  30          


Thr Ala Ala Val Gly Leu Phe Asn Pro Lys Val Gly Lys Val Ser Arg 
        35                  40                  45              


Val Val Ala Lys Ser Ala Asn Ala Lys Pro Ala Glu Arg Thr Gln Ser 
    50                  55                  60                  


Gly Ala Ala Met Thr Ala Phe Val Phe Val His Asn Leu Ile Leu Cys 
65                  70                  75                  80  


Val Tyr Ser Gly Ile Thr Phe Tyr Tyr Met Phe Pro Ala Met Val Lys 
                85                  90                  95      


Asn Phe Arg Thr His Thr Leu His Glu Ala Tyr Cys Asp Thr Asp Gln 
            100                 105                 110         


Ser Leu Trp Asn Asn Ala Leu Gly Tyr Trp Gly Tyr Leu Phe Tyr Leu 
        115                 120                 125             


Ser Lys Phe Tyr Glu Val Ile Asp Thr Ile Ile Ile Ile Leu Lys Gly 
    130                 135                 140                 


Arg Arg Ser Ser Leu Leu Gln Thr Tyr His His Ala Gly Ala Met Ile 
145                 150                 155                 160 


Thr Met Trp Ser Gly Ile Asn Tyr Gln Ala Thr Pro Ile Trp Ile Phe 
                165                 170                 175     


Val Val Phe Asn Ser Phe Ile His Thr Ile Met Tyr Cys Tyr Tyr Ala 
            180                 185                 190         


Phe Thr Ser Ile Gly Phe His Pro Pro Gly Lys Lys Tyr Leu Thr Ser 
        195                 200                 205             


Met Gln Ile Thr Gln Phe Leu Val Gly Ile Thr Ile Ala Val Ser Tyr 
    210                 215                 220                 


Leu Phe Val Pro Gly Cys Ile Arg Thr Pro Gly Ala Gln Met Ala Val 
225                 230                 235                 240 


Trp Ile Asn Val Gly Tyr Leu Phe Pro Leu Thr Tyr Leu Phe Val Asp 
                245                 250                 255     


Phe Ala Lys Arg Thr Tyr Ser Lys Arg Ser Ala Ile Ala Ala Gln Lys 
            260                 265                 270         


Lys Ala Gln 
        275 


<210>  36
<211>  8739
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pY116

<400>  36
ggccgccacc gcggcccgag attccggcct cttcggccgc caagcgaccc gggtggacgt     60

ctagaggtac ctagcaatta acagatagtt tgccggtgat aattctctta acctcccaca    120

ctcctttgac ataacgattt atgtaacgaa actgaaattt gaccagatat tgtgtccgcg    180

gtggagctcc agcttttgtt ccctttagtg agggtttaaa cgagcttggc gtaatcatgg    240

tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa cgtacgagcc    300

ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg    360

ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc    420

ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact    480

gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta    540

atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag    600

caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc    660

cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta    720

taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg    780

ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc    840

tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac    900

gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac    960

ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg   1020

aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga   1080

aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt   1140

agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag   1200

cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct   1260

gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg   1320

atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat   1380

gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc   1440

tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg   1500

gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct   1560

ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca   1620

actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg   1680

ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg   1740

tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc   1800

cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag   1860

ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg   1920

ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag   1980

tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat   2040

agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg   2100

atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca   2160

gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca   2220

aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat   2280

tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag   2340

aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc   2400

tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt   2460

gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc   2520

ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta   2580

cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc   2640

tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg   2700

ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt   2760

ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat   2820

tttaacaaaa tattaacgct tacaatttcc attcgccatt caggctgcgc aactgttggg   2880

aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg   2940

caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg   3000

ccagtgaatt gtaatacgac tcactatagg gcgaattggg taccgggccc cccctcgagg   3060

tcgatggtgt cgataagctt gatatcgaat tcatgtcaca caaaccgatc ttcgcctcaa   3120

ggaaacctaa ttctacatcc gagagactgc cgagatccag tctacactga ttaattttcg   3180

ggccaataat ttaaaaaaat cgtgttatat aatattatat gtattatata tatacatcat   3240

gatgatactg acagtcatgt cccattgcta aatagacaga ctccatctgc cgcctccaac   3300

tgatgttctc aatatttaag gggtcatctc gcattgttta ataataaaca gactccatct   3360

accgcctcca aatgatgttc tcaaaatata ttgtatgaac ttatttttat tacttagtat   3420

tattagacaa cttacttgct ttatgaaaaa cacttcctat ttaggaaaca atttataatg   3480

gcagttcgtt catttaacaa tttatgtaga ataaatgtta taaatgcgta tgggaaatct   3540

taaatatgga tagcataaat gatatctgca ttgcctaatt cgaaatcaac agcaacgaaa   3600

aaaatccctt gtacaacata aatagtcatc gagaaatatc aactatcaaa gaacagctat   3660

tcacacgtta ctattgagat tattattgga cgagaatcac acactcaact gtctttctct   3720

cttctagaaa tacaggtaca agtatgtact attctcattg ttcatacttc tagtcatttc   3780

atcccacata ttccttggat ttctctccaa tgaatgacat tctatcttgc aaattcaaca   3840

attataataa gatataccaa agtagcggta tagtggcaat caaaaagctt ctctggtgtg   3900

cttctcgtat ttatttttat tctaatgatc cattaaaggt atatatttat ttcttgttat   3960

ataatccttt tgtttattac atgggctgga tacataaagg tattttgatt taattttttg   4020

cttaaattca atcccccctc gttcagtgtc aactgtaatg gtaggaaatt accatacttt   4080

tgaagaagca aaaaaaatga aagaaaaaaa aaatcgtatt tccaggttag acgttccgca   4140

gaatctagaa tgcggtatgc ggtacattgt tcttcgaacg taaaagttgc gctccctgag   4200

atattgtaca tttttgcttt tacaagtaca agtacatcgt acaactatgt actactgttg   4260

atgcatccac aacagtttgt tttgtttttt tttgtttttt ttttttctaa tgattcatta   4320

ccgctatgta tacctacttg tacttgtagt aagccgggtt attggcgttc aattaatcat   4380

agacttatga atctgcacgg tgtgcgctgc gagttacttt tagcttatgc atgctacttg   4440

ggtgtaatat tgggatctgt tcggaaatca acggatgctc aaccgatttc gacagtaatt   4500

aattaatttg aatcgaatcg gagcctaaaa tgaacccgag tatatctcat aaaattctcg   4560

gtgagaggtc tgtgactgtc agtacaaggt gccttcatta tgccctcaac cttaccatac   4620

ctcactgaat gtagtgtacc tctaaaaatg aaatacagtg ccaaaagcca aggcactgag   4680

ctcgtctaac ggacttgata tacaaccaat taaaacaaat gaaaagaaat acagttcttt   4740

gtatcatttg taacaattac cctgtacaaa ctaaggtatt gaaatcccac aatattccca   4800

aagtccaccc ctttccaaat tgtcatgcct acaactcata taccaagcac taacctacca   4860

aacaccacta aaaccccaca aaatatatct taccgaatat acagtaacaa gctaccacca   4920

cactcgttgg gtgcagtcgc cagcttaaag atatctatcc acatcagcca caactccctt   4980

cctttaataa accgactaca cccttggcta ttgaggttat gagtgaatat actgtagaca   5040

agacactttc aagaagactg tttccaaaac gtaccactgt cctccactac aaacacaccc   5100

aatctgcttc ttctagtcaa ggttgctaca ccggtaaatt ataaatcatc atttcattag   5160

cagggcaggg ccctttttat agagtcttat acactagcgg accctgccgg tagaccaacc   5220

cgcaggcgcg tcagtttgct ccttccatca atgcgtcgta gaaacgactt actccttctt   5280

gagcagctcc ttgaccttgt tggcaacaag tctccgacct cggaggtgga ggaagagcct   5340

ccgatatcgg cggtagtgat accagcctcg acggactcct tgacggcagc ctcaacagcg   5400

tcaccggcgg gcttcatgtt aagagagaac ttgagcatca tggcggcaga cagaatggtg   5460

gcaatggggt tgaccttctg cttgccgaga tcgggggcag atccgtgaca gggctcgtac   5520

agaccgaacg cctcgttggt gtcgggcaga gaagccagag aggcggaggg cagcagaccc   5580

agagaaccgg ggatgacgga ggcctcgtcg gagatgatat cgccaaacat gttggtggtg   5640

atgatgatac cattcatctt ggagggctgc ttgatgagga tcatggcggc cgagtcgatc   5700

agctggtggt tgagctcgag ctgggggaat tcgtccttga ggactcgagt gacagtcttt   5760

cgccaaagtc gagaggaggc cagcacgttg gccttgtcaa gagaccacac gggaagaggg   5820

gggttgtgct gaagggccag gaaggcggcc attcgggcaa ttcgctcaac ctcaggaacg   5880

gagtaggtct cggtgtcgga agcgacgcca gatccgtcat cctcctttcg ctctccaaag   5940

tagatacctc cgacgagctc tcggacaatg atgaagtcgg tgccctcaac gtttcggatg   6000

ggggagagat cggcgagctt gggcgacagc agctggcagg gtcgcaggtt ggcgtacagg   6060

ttcaggtcct ttcgcagctt gaggagaccc tgctcgggtc gcacgtcggt tcgtccgtcg   6120

ggagtggtcc atacggtgtt ggcagcgcct ccgacagcac cgagcataat agagtcagcc   6180

tttcggcaga tgtcgagagt agcgtcggtg atgggctcgc cctccttctc aatggcagct   6240

cctccaatga gtcggtcctc aaacacaaac tcggtgccgg aggcctcagc aacagacttg   6300

agcaccttga cggcctcggc aatcacctcg gggccacaga agtcgccgcc gagaagaaca   6360

atcttcttgg agtcagtctt ggtcttctta gtttcgggtt ccattgtgga tgtgtgtggt   6420

tgtatgtgtg atgtggtgtg tggagtgaaa atctgtggct ggcaaacgct cttgtatata   6480

tacgcacttt tgcccgtgct atgtggaaga ctaaacctcc gaagattgtg actcaggtag   6540

tgcggtatcg gctagggacc caaaccttgt cgatgccgat agcgctatcg aacgtacccc   6600

agccggccgg gagtatgtcg gaggggacat acgagatcgt caagggtttg tggccaactg   6660

gtatttaaat gtagctaacg gtagcaggcg aactactggt acatacctcc cccggaatat   6720

gtacaggcat aatgcgtatc tgtgggacat gtggtcgttg cgccattatg taagcagcgt   6780

gtactcctct gactgtccat atggtttgct ccatctcacc ctcatcgttt tcattgttca   6840

caggcggcca caaaaaaact gtcttctctc cttctctctt cgccttagtc tactcggacc   6900

agttttagtt tagcttggcg ccactggata aatgagacct caggccttgt gatgaggagg   6960

tcacttatga agcatgttag gaggtgcttg tatggataga gaagcaccca aaataataag   7020

aataataata aaacaggggg cgttgtcatt tcatatcgtg ttttcaccat caatacacct   7080

ccaaacaatg cccttcatgt ggccagcccc aatattgtcc tgtagttcaa ctctatgcag   7140

ctcgtatctt attgagcaag taaaactctg tcagccgata ttgcccgacc cgcgacaagg   7200

gtcaacaagg tggtgtaagg ccttcgcaga agtcaaaact gtgccaaaca aacatctaga   7260

gtctctttgg tgtttctcgc atatatttwa tcggctgtct tacgtatttg cgcctcggta   7320

ccggactaat ttcggatcat ccccaatacg ctttttcttc gcagctgtca acagtgtcca   7380

tgatctatcc acctaaatgg gtcatatgag gcgtataatt tcgtggtgct gataataatt   7440

cccatatatt tgacacaaaa cttccccccc tagacataca tctcacaatc tcacttcttg   7500

tgcttctgtc acacatctcc tccagctgac ttcaactcac acctctgccc cagttggtct   7560

acagcggtat aaggtttctc cgcatagagg tgcaccactc ctcccgatac ttgtttgtgt   7620

gacttgtggg tcacgacata tatatctaca cacattgcgc caccctttgg ttcttccagc   7680

acaacaaaaa cacgacacgc taaccatggc caatttactg accgtacacc aaaatttgcc   7740

tgcattaccg gtcgatgcaa cgagtgatga ggttcgcaag aacctgatgg acatgttcag   7800

ggatcgccag gcgttttctg agcatacctg gaaaatgctt ctgtccgttt gccggtcgtg   7860

ggcggcatgg tgcaagttga ataaccggaa atggtttccc gcagaacctg aagatgttcg   7920

cgattatctt ctatatcttc aggcgcgcgg tctggcagta aaaactatcc agcaacattt   7980

gggccagcta aacatgcttc atcgtcggtc cgggctgcca cgaccaagtg acagcaatgc   8040

tgtttcactg gttatgcggc ggatccgaaa agaaaacgtt gatgccggtg aacgtgcaaa   8100

acaggctcta gcgttcgaac gcactgattt cgaccaggtt cgttcactca tggaaaatag   8160

cgatcgctgc caggatatac gtaatctggc atttctgggg attgcttata acaccctgtt   8220

acgtatagcc gaaattgcca ggatcagggt taaagatatc tcacgtactg acggtgggag   8280

aatgttaatc catattggca gaacgaaaac gctggttagc accgcaggtg tagagaaggc   8340

acttagcctg ggggtaacta aactggtcga gcgatggatt tccgtctctg gtgtagctga   8400

tgatccgaat aactacctgt tttgccgggt cagaaaaaat ggtgttgccg cgccatctgc   8460

caccagccag ctatcaactc gcgccctgga agggattttt gaagcaactc atcgattgat   8520

ttacggcgct aaggatgact ctggtcagag atacctggcc tggtctggac acagtgcccg   8580

tgtcggagcc gcgcgagata tggcccgcgc tggagtttca ataccggaga tcatgcaagc   8640

tggtggctgg accaatgtaa atattgtcat gaactatatc cgtaacctgg atagtgaaac   8700

aggggcaatg gtgcgcctgc tggaagatgg cgattaagc                          8739


<210>  37
<211>  15337
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pKO2UF8289

<400>  37
cgatcgagga agaggacaag cggctgcttc ttaagtttgt gacatcagta tccaaggcac     60

cattgcaagg attcaaggct ttgaacccgt catttgccat tcgtaacgct ggtagacagg    120

ttgatcggtt ccctacggcc tccacctgtg tcaatcttct caagctgcct gactatcagg    180

acattgatca acttcggaag aaacttttgt atgccattcg atcacatgct ggtttcgatt    240

tgtcttagag gaacgcatat acagtaatca tagagaataa acgatattca tttattaaag    300

tagatagttg aggtagaagt tgtaaagagt gataaatagc ggccgctcac tgaatctttt    360

tggctccctt gtgcttcctg acgatatacg tttgcacata gaaattcaag aacaaacaca    420

agactgtgcc aacataaaag taattgaaga accagccaaa catcctcatc ccatcttggc    480

gataacaggg aatgttcctg tacttccaga caatgtagaa accaacattg aattgaatga    540

tctgcattga tgtaatcagg gattttggca tggggaactt cagcttgatc aatctggtcc    600

aataataacc gtacatgatc cagtggatga aaccattcaa cagcacaaaa atccaaacag    660

cttcatttcg gtaattatag aacagccaca tatccatcgg tgcccccaaa tgatggaaga    720

attgcaacca ggtcagaggc ttgcccatca gtggcaaata gaaggagtca atatactcca    780

ggaacttgct caaatagaac aactgcgtgg tgatcctgaa gacgttgttg tcaaaagcct    840

tctcgcagtt gtcagacata acaccgatgg tgtacatggc atatgccatt gagaggaatg    900

atcccaacga ataaatggac atgagaaggt tgtaattggt gaaaacaaac ttcatacgag    960

actgaccttt tggaccaagg gggccaagag tgaacttcaa gatgacaaat gcgatggaca   1020

agtaaagcac ctcacagtga ctggcatcac tccagagttg ggcataatca actggttggg   1080

taaaacttcc tgcccaattg agactatttc attcaccacc tccatggtta gcgtgtcgtg   1140

tttttgttgt gctggaagaa ccaaagggtg gcgcaatgtg tgtagatata tatgtcgtga   1200

cccacaagtc acacaaacaa gtatcgggag gagtggtgca cctctatgcg gagaaacctt   1260

ataccgctgt agaccaactg gggcagaggt gtgagttgaa gtcagctgga ggagatgtgt   1320

gacagaagca caagaagtga gattgtgaga tgtatgtcta gggggggaag ttttgtgtca   1380

aatatatggg aattattatc agcaccacga aattatacgc ctcatatgac ccatttaggt   1440

ggatagatca tggacactgt tgacagctgc gaagaaaaag cgtattgggg atgatccgaa   1500

attagtccgg taccgaggcg caaatacgta agacagccga twaaatatat gcgagaaaca   1560

ccaaagagac tctagatgtt tgtttggcac agttttgact tctgcgaagg ccttacacca   1620

ccttgttgac ccttgtcgcg ggtcgggcaa tatcggctga cagagtttta cttgctcaat   1680

aagatacgag ctgcatagag ttgaactaca ggacaatatt ggggctggcc acatgaaggg   1740

cattgtttgg aggtgtattg atggtgaaaa cacgatatga aatgacaacg ccccctgttt   1800

tattattatt cttattattt tgggtgcttc tctatccata caagcacctc ctaacatgct   1860

tcataagtga cctcctcatc acaaggcctg aggtctcatt tatccagtgg cgccaagcta   1920

aactaaaact ggtccgagta gactaaggcg aagagagaag gagagaagac agtttttttg   1980

tggccgcctg tgaacaatga aaacgatgag ggtgagatgg agcaaaccat atggtttaaa   2040

cagtcagagg agtacacgct gcttacataa tggcgcaacg accacatgtc ccacagatac   2100

gcatcgattc gattcaaatt aattaaaagg cgttgaaaca gaatgagcca gacagcaagg   2160

acaaggtggc caacagcaag gagtccaaaa agccctctat tgacgagatc cacgatgtta   2220

ttgctcatga ggtttccgag ctcgatgctg ggaagaagaa gtgatttgta tataagaaat   2280

aaatgagata tagtaaagga gtgcaagaga atggcaaggt ggtcaaattc tatattactt   2340

gcagtcactg gttcctcgtt gacatgaatg aagttaccgt tggcatagct gatttaatat   2400

ataactgtcc aactaactct cacctagata taacccatgt gtgtgtttcc aatcatcaat   2460

gcggccgctt actgagcctt ggcaccgggc tgcttctcgg ccattcgagc gaactgggac   2520

aggtatcgga gcaggatgac gagaccttca tggggcagag ggtttcggta ggggaggttg   2580

tgcttctggc acagctgttc cacctggtag gaaacggcag tgaggttgtg tcgaggcagg   2640

gtgggccaga gatggtgctc gatctggtag ttcaggcctc caaagaacca gtcagtaatg   2700

atgcctcgtc gaatgttcat ggtctcatgg atctgaccca cagagaagcc atgtccgtcc   2760

cagacggaat caccgatctt ctccagaggg tagtggttca tgaagaccac gatggcaatt   2820

ccgaagccac cgacgagctc ggaaacaaag aacaccagca tcgaggtcag gatggagggc   2880

ataaagaaga ggtggaacag ggtcttgaga gtccagtgca gagcgagtcc aatggcctct   2940

ttcttgtact gagatcggta gaactggttg tctcggtcct tgagggatcg aacggtcagc   3000

acagactgga aacaccagat gaatcgcagg agaatacaga tgaccaggaa atagtactgt   3060

tggaactgaa tgagctttcg ggagatggga gaagctcgag tgacatcgtc ctcggaccag   3120

gcgagcagag gcaggttatc aatgtcggga tcgtgaccct gaacgttggt agcagaatga   3180

tgggcgttgt gtctgtcctt ccaccaggtc acggagaagc cctggagtcc gttgccaaag   3240

accagaccca ggacgttatt ccagtttcgg ttcttgaagg tctggtggtg gcagatgtca   3300

tgagacagcc atcccatttg ctggtagtgc ataccgagca cgagagcacc aatgaagtac   3360

aggtggtact ggaccagcat gaagaaggca agcacgccaa gacccagggt ggtcaagatc   3420

ttgtacgagt accagagggg agaggcgtca aacatgccag tggcgatcag ctcttctcgg   3480

agctttcgga aatcctcctg agcttcgttg acggcagcct ggggaggcag ctcggaagcc   3540

tggttgatct tgggcattcg cttgagcttg tcgaaggctt cctgagagtg cataaccatg   3600

aaggcgtcag tagcatctcg tccctggtag ttctcaatga tttcagctcc accagggtgg   3660

aagttcaccc aagcggagac gtcgtacacc tttccgtcga tgacgagggg cagagcctgt   3720

cgagaagcct tcaccatggc cattgctgta gatatgtctt gtgtgtaagg gggttggggt   3780

ggttgtttgt gttcttgact tttgtgttag caagggaaga cgggcaaaaa agtgagtgtg   3840

gttgggaggg agagacgagc cttatatata atgcttgttt gtgtttgtgc aagtggacgc   3900

cgaaacgggc aggagccaaa ctaaacaagg cagacaatgc gagcttaatt ggattgcctg   3960

atgggcaggg gttagggctc gatcaatggg ggtgcgaagt gacaaaattg ggaattaggt   4020

tcgcaagcaa ggctgacaag actttggccc aaacatttgt acgcggtgga caacaggagc   4080

cacccatcgt ctgtcacggg ctagccggtc gtgcgtcctg tcaggctcca cctaggctcc   4140

atgccactcc atacaatccc actagtgtac cgctaggccg cttttagctc ccatctaaga   4200

cccccccaaa acctccactg tacagtgcac tgtactgtgt ggcgatcaag ggcaagggaa   4260

aaaaggcgca aacatgcacg catggaatga cgtaggtaag gcgttactag actgaaaagt   4320

ggcacatttc ggcgtgccaa agggtcctag gtgcgtttcg cgagctgggc gccaggccaa   4380

gccgctccaa aacgcctctc cgactccctc cagcggcctc catatcccca tccctctcca   4440

cagcaatgtt gttaagcctt gcaaacgaaa aaatagaaag gctaataagc ttccaatatt   4500

gtggtgtacg ctgcataacg caacaatgag cgccaaacaa cacacacaca cagcacacag   4560

cagcattaac cacgatgttt aaacagtgta cgcagatccc gtcaacagtt ttatatatcg   4620

tagttacaac catcaacact ttttggtaag tgtaccattc tatactccaa ctggtctgca   4680

actgtacaag tagacatgtt aatggtagtt aataacatct acagcagaac ctatggtaaa   4740

gacattgcat ttttacagga agtatcgtcc tacacgttga taaatccaaa gatgcggaac   4800

ttcttccact tttatcatca tcccctactc gtacactcgt actctttgtt cgatcgcgat   4860

tcatttctat aaataatctt gtatgtacat gcggccgctt aagcaacggg cttgataaca   4920

gcgggggggg tgcccacgtt gttgcggttg cggaagaaca gaacaccctt accagcaccc   4980

tcggcaccag cgctgggctc aacccactgg cacatacgcg cactgcggta catggcgcgg   5040

atgaagccac gaggaccatc ctggacatca gcccggtagt gcttgcccat gatgggctta   5100

atggcctcgg tggcctcgtc cgcgttgtag aaggggatgc tgctgacgta gtggtggagg   5160

acatgagtct cgatgatgcc gtggagaagg tggcggccga tgaagcccat ctcacggtca   5220

atggtagcag cggcaccacg gacgaagttc cactcgtcgt tggtgtagtg gggaagggta   5280

gggtcggtgt gctggaggaa ggtgatggca acgagccagt ggttaaccca gaggtaggga   5340

acaaagtacc agatggccat gttgtagaaa ccgaacttct gaacgaggaa gtacagagca   5400

gtggccatca gaccgatacc aatatcgctg aggacgatga gcttagcgtc actgttctcg   5460

tacagagggc tgcggggatc gaagtggtta acaccaccgc cgaggccgtt atgcttgccc   5520

ttgccgcgac cctcacgctg gcgctcgtgg tagttgtggc cggtaacatt ggtgatgagg   5580

tagttgggcc agccaacgag ctgctgaagg acgagcatga gaagagtgaa agcgggggtc   5640

tcctcagtaa gatgagcgag ctcgtgggtc atctttccga gacgagtagc ctgctgctcg   5700

cgggttcggg gaacgaagac catgtcacgc tccatgttgc cagtggcctt gtggtgcttt   5760

cggtgggaga tttgccagct gaagtagggg acaaggaggg aagagtgaag aacccagcca   5820

gtaatgtcgt tgatgatgcg agaatcggag aaagcaccgt gaccgcactc atgggcaata   5880

acccagagac cagtaccgaa aagaccctga agaacggtgt acacggccca cagaccagcg   5940

cgggcggggg tggaggggat atattcgggg gtcacaaagt tgtaccagat gctgaaagtg   6000

gtagtcagga ggacaatgtc gcggaggata taaccgtatc ccttgagagc ggagcgcttg   6060

aagcagtgct tagggatggc attgtagatg tccttgatgg taaagtcggg aacctcgaac   6120

tggttgccgt aggtgtcgag catgacacca tactcggact tgggcttggc gatatcaacc   6180

tcggacatgg acgagagcga tgtggaagag gccgagtggc ggggagagtc tgaaggagag   6240

acggcggcag actcagaatc cgtcacagta gttgaggtga cggtgcgtct aagcgcaggg   6300

ttctgcttgg gcagagccga agtggacgcc atggttgtga attagggtgg tgagaatggt   6360

tggttgtagg gaagaatcaa aggccggtct cgggatccgt gggtatatat atatatatat   6420

atatatacga tccttcgtta cctccctgtt ctcaaaactg tggtttttcg tttttcgttt   6480

tttgcttttt ttgatttttt tagggccaac taagcttcca gatttcgcta atcacctttg   6540

tactaattac aagaaaggaa gaagctgatt agagttgggc tttttatgca actgtgctac   6600

tccttatctc tgatatgaaa gtgtagaccc aatcacatca tgtcatttag agttggtaat   6660

actgggagga tagataaggc acgaaaacga gccatagcag acatgctggg tgtagccaag   6720

cagaagaaag tagatgggag ccaattgacg agcgagggag ctacgccaat ccgacatacg   6780

acacgctgag atcgtcttgg ccggggggta cctacagatg tccaagggta agtgcttgac   6840

tgtaattgta tgtctgagga caaatatgta gtcagccgta taaagtcata ccaggcacca   6900

gtgccatcat cgaaccacta actctctatg atacatgcct ccggtattat tgtaccatgc   6960

gtcgctttgt tacatacgta tcttgccttt ttctctcaga aactccagac tttggctatt   7020

ggtcgagata agcccggacc atagtgagtc tttcacactc tacatttctc ccttgctcca   7080

actatttaaa ttgccccgga gaagacggcc aggccgccta gatgacaaat tcaacaactc   7140

acagctgact ttctgccatt gccactaggg gggggccttt ttatatggcc aagccaagct   7200

ctccacgtcg gttgggctgc acccaacaat aaatgggtag ggttgcacca acaaagggat   7260

gggatggggg gtagaagata cgaggataac ggggctcaat ggcacaaata agaacgaata   7320

ctgccattaa gactcgtgat ccagcgactg acaccattgc atcatctaag ggcctcaaaa   7380

ctacctcgga actgctgcgc tgatctggac accacagagg ttccgagcac tttaggttgc   7440

accaaatgtc ccaccaggtg caggcagaaa acgctggaac agcgtgtaca gtttgtctta   7500

acaaaaagtg agggcgctga ggtcgagcag ggtggtgtga cttgttatag cctttagagc   7560

tgcgaaagcg cgtatggatt tggctcatca ggccagattg agggtctgtg gacacatgtc   7620

atgttagtgt acttcaatcg ccccctggat atagccccga caataggccg tggcctcatt   7680

tttttgcctt ccgcacattt ccattgctcg gtacccacac cttgcttctc ctgcacttgc   7740

caaccttaat actggtttac attgaccaac atcttacaag cggggggctt gtctagggta   7800

tatataaaca gtggctctcc caatcggttg ccagtctctt ttttcctttc tttccccaca   7860

gattcgaaat ctaaactaca catcacagaa ttccgagccg tgagtatcca cgacaagatc   7920

agtgtcgaga cgacgcgttt tgtgtaatga cacaatccga aagtcgctag caacacacac   7980

tctctacaca aactaaccca gctctggtac catggtgaag gcttctcgac aggctctgcc   8040

cctcgtcatc gacggaaagg tgtacgacgt ctccgcttgg gtgaacttcc accctggtgg   8100

agctgaaatc attgagaact accagggacg agatgctact gacgccttca tggttatgca   8160

ctctcaggaa gccttcgaca agctcaagcg aatgcccaag atcaaccagg cttccgagct   8220

gcctccccag gctgccgtca acgaagctca ggaggatttc cgaaagctcc gagaagagct   8280

gatcgccact ggcatgtttg acgcctctcc cctctggtac tcgtacaaga tcttgaccac   8340

cctgggtctt ggcgtgcttg ccttcttcat gctggtccag taccacctgt acttcattgg   8400

tgctctcgtg ctcggtatgc actaccagca aatgggatgg ctgtctcatg acatctgcca   8460

ccaccagacc ttcaagaacc gaaactggaa taacgtcctg ggtctggtct ttggcaacgg   8520

actccagggc ttctccgtga cctggtggaa ggacagacac aacgcccatc attctgctac   8580

caacgttcag ggtcacgatc ccgacattga taacctgcct ctgctcgcct ggtccgagga   8640

cgatgtcact cgagcttctc ccatctcccg aaagctcatt cagttccaac agtactattt   8700

cctggtcatc tgtattctcc tgcgattcat ctggtgtttc cagtctgtgc tgaccgttcg   8760

atccctcaag gaccgagaca accagttcta ccgatctcag tacaagaaag aggccattgg   8820

actcgctctg cactggactc tcaagaccct gttccacctc ttctttatgc cctccatcct   8880

gacctcgatg ctggtgttct ttgtttccga gctcgtcggt ggcttcggaa ttgccatcgt   8940

ggtcttcatg aaccactacc ctctggagaa gatcggtgat tccgtctggg acggacatgg   9000

cttctctgtg ggtcagatcc atgagaccat gaacattcga cgaggcatca ttactgactg   9060

gttctttgga ggcctgaact accagatcga gcaccatctc tggcccaccc tgcctcgaca   9120

caacctcact gccgtttcct accaggtgga acagctgtgc cagaagcaca acctccccta   9180

ccgaaaccct ctgccccatg aaggtctcgt catcctgctc cgatacctgt cccagttcgc   9240

tcgaatggcc gagaagcagc ccggtgccaa ggctcagtaa gcggccgcaa gtgtggatgg   9300

ggaagtgagt gcccggttct gtgtgcacaa ttggcaatcc aagatggatg gattcaacac   9360

agggatatag cgagctacgt ggtggtgcga ggatatagca acggatattt atgtttgaca   9420

cttgagaatg tacgatacaa gcactgtcca agtacaatac taaacatact gtacatactc   9480

atactcgtac ccgggcaacg gtttcacttg agtgcagtgg ctagtgctct tactcgtaca   9540

gtgtgcaata ctgcgtatca tagtctttga tgtatatcgt attcattcat gttagttgcg   9600

tacgggtgaa gcttccactg gtcggcgtgg tagtggggca gagtggggtc ggtgtgctgc   9660

aggtaggtga tggccacgag ccagtggttg acccacaggt aggggatcag gtagtagagg   9720

gtgacggaag ccaggcccca tcggttgatg gagtatgcga tgacggacat ggtgatacca   9780

ataccgacgt tagagatcca gatgttgaac cagtccttct tctcaaacag cggggcgttg   9840

gggttgaagt ggttgacagc ccatttgttg agcttggggt acttctgtcc ggtaacgtaa   9900

gacagcagat acagaggcca tccaaacacc tgctgggtga tgaggccgta gagggtcatg   9960

aggggagcgt cctcagcaag ctcagaccag tcatgggcgc ctcggttctc cataaactcc  10020

tttcggtcct tgggcacaaa caccatatca cgggtgaggt gaccagtgga cttgtggtgc  10080

atggagtggg tcagcttcca ggcgtagtaa gggaccagca tggaggagtg cagaacccat  10140

ccggtgacgt tgttgacggt gttagagtcg gagaaagcag agtggccaca ctcgtgggca  10200

agaacccaca gaccggtgcc aaacagaccc tggacaatgg agtacatggc ccaggccaca  10260

gctcggccgg aagccgaggg aataagaggc aggtacgcgt aggccatgta ggcaaaaacg  10320

gcgataaaga agcaggcgcg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc  10380

ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt  10440

cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca  10500

ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa  10560

aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat  10620

cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc  10680

cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc  10740

gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt  10800

tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac  10860

cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg  10920

ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca  10980

gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc  11040

gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa  11100

accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa  11160

ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac  11220

tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta  11280

aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt  11340

taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata  11400

gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc  11460

agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac  11520

cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag  11580

tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac  11640

gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc  11700

agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg  11760

gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc  11820

atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct  11880

gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc  11940

tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc  12000

atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc  12060

agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc  12120

gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca  12180

cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt  12240

tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt  12300

ccgcgcacat ttccccgaaa agtgccacct gatgcggtgt gaaataccgc acagatgcgt  12360

aaggagaaaa taccgcatca ggaaattgta agcgttaata ttttgttaaa attcgcgtta  12420

aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat  12480

aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca  12540

ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc  12600

ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta  12660

aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg  12720

gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg  12780

gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc  12840

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat  12900

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt  12960

tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac tcactatagg  13020

gcgaattggg cccgacgtcg catgcttgaa tctacaagta ggagggttgg agtgattaag  13080

tgaaacttct ttaacggctc tatgccagtt ctattgatat ccgaaacatc agtatgaagg  13140

tctgataagg gtgacttctt cccacagatt cgtatcagta cgagtacgag accggtactt  13200

gtaacagtat tgatactaaa gggaaactac aacggttgtc agcgtaatgt gacttcgccc  13260

atgaacgcag acacgcagtg ccgagtgcgg tgatatcgcc tactcgttac gtccatggac  13320

tacacaaccc ctcggcttcg cttggcttag cctcgggctc ggtgctgttc agttaaaaca  13380

caatcaaata acatttctac tttttagaag gcaggccgtc aggagcaact ccgactccat  13440

tgacgtttct aaacatctga atgccttcct taccttcaac aaactggcag gttcgggcga  13500

cagtgtaaag agacttgatg aagttggtgt cgtcgtgtcg gtagtgcttg cccatgacct  13560

tcttgatctt ctcagtggcg attcgggcgt tgtagaaggg aattccttta cctgcaggat  13620

aacttcgtat aatgtatgct atacgaagtt atgatctctc tcttgagctt ttccataaca  13680

agttcttctg cctccaggaa gtccatgggt ggtttgatca tggttttggt gtagtggtag  13740

tgcagtggtg gtattgtgac tggggatgta gttgagaata agtcatacac aagtcagctt  13800

tcttcgagcc tcatataagt ataagtagtt caacgtatta gcactgtacc cagcatctcc  13860

gtatcgagaa acacaacaac atgccccatt ggacagatca tgcggataca caggttgtgc  13920

agtatcatac atactcgatc agacaggtcg tctgaccatc atacaagctg aacaagcgct  13980

ccatacttgc acgctctcta tatacacagt taaattacat atccatagtc taacctctaa  14040

cagttaatct tctggtaagc ctcccagcca gccttctggt atcgcttggc ctcctcaata  14100

ggatctcggt tctggccgta cagacctcgg ccgacaatta tgatatccgt tccggtagac  14160

atgacatcct caacagttcg gtactgctgt ccgagagcgt ctcccttgtc gtcaagaccc  14220

accccggggg tcagaataag ccagtcctca gagtcgccct taggtcggtt ctgggcaatg  14280

aagccaacca caaactcggg gtcggatcgg gcaagctcaa tggtctgctt ggagtactcg  14340

ccagtggcca gagagccctt gcaagacagc tcggccagca tgagcagacc tctggccagc  14400

ttctcgttgg gagaggggac taggaactcc ttgtactggg agttctcgta gtcagagacg  14460

tcctccttct tctgttcaga gacagtttcc tcggcaccag ctcgcaggcc agcaatgatt  14520

ccggttccgg gtacaccgtg ggcgttggtg atatcggacc actcggcgat tcggtgacac  14580

cggtactggt gcttgacagt gttgccaata tctgcgaact ttctgtcctc gaacaggaag  14640

aaaccgtgct taagagcaag ttccttgagg gggagcacag tgccggcgta ggtgaagtcg  14700

tcaatgatgt cgatatgggt tttgatcatg cacacataag gtccgacctt atcggcaagc  14760

tcaatgagct ccttggtggt ggtaacatcc agagaagcac acaggttggt tttcttggct  14820

gccacgagct tgagcactcg agcggcaaag gcggacttgt ggacgttagc tcgagcttcg  14880

taggagggca ttttggtggt gaagaggaga ctgaaataaa tttagtctgc agaacttttt  14940

atcggaacct tatctggggc agtgaagtat atgttatggt aatagttacg agttagttga  15000

acttatagat agactggact atacggctat cggtccaaat tagaaagaac gtcaatggct  15060

ctctgggcgt cgcctttgcc gacaaaaatg tgatcatgat gaaagccagc aatgacgttg  15120

cagctgatat tgttgtcggc caaccgcgcc gaaaacgcag ctgtcagacc cacagcctcc  15180

aacgaagaat gtatcgtcaa agtgatccaa gcacactcat agttggagtc gtactccaaa  15240

ggcggcaatg acgagtcaga cagatactcg tcgacgcgat aacttcgtat aatgtatgct  15300

atacgaagtt atcgtacgat agttagtaga caacaat                           15337


<210>  38
<211>  1272
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutant EgD8M delta-8 desaturase (also designated as "EgD8S-23")


<220>
<221>  CDS
<222>  (2)..(1270)

<400>  38
c atg gtg aag gct tct cga cag gct ctg ccc ctc gtc atc gac gga aag     49
  Met Val Lys Ala Ser Arg Gln Ala Leu Pro Leu Val Ile Asp Gly Lys       
  1               5                   10                  15            

gtg tac gac gtc tcc gct tgg gtg aac ttc cac cct ggt gga gct gaa       97
Val Tyr Asp Val Ser Ala Trp Val Asn Phe His Pro Gly Gly Ala Glu         
            20                  25                  30                  

atc att gag aac tac cag gga cga gat gct act gac gcc ttc atg gtt      145
Ile Ile Glu Asn Tyr Gln Gly Arg Asp Ala Thr Asp Ala Phe Met Val         
        35                  40                  45                      

atg cac tct cag gaa gcc ttc gac aag ctc aag cga atg ccc aag atc      193
Met His Ser Gln Glu Ala Phe Asp Lys Leu Lys Arg Met Pro Lys Ile         
    50                  55                  60                          

aac cag gct tcc gag ctg cct ccc cag gct gcc gtc aac gaa gct cag      241
Asn Gln Ala Ser Glu Leu Pro Pro Gln Ala Ala Val Asn Glu Ala Gln         
65                  70                  75                  80          

gag gat ttc cga aag ctc cga gaa gag ctg atc gcc act ggc atg ttt      289
Glu Asp Phe Arg Lys Leu Arg Glu Glu Leu Ile Ala Thr Gly Met Phe         
                85                  90                  95              

gac gcc tct ccc ctc tgg tac tcg tac aag atc ttg acc acc ctg ggt      337
Asp Ala Ser Pro Leu Trp Tyr Ser Tyr Lys Ile Leu Thr Thr Leu Gly         
            100                 105                 110                 

ctt ggc gtg ctt gcc ttc ttc atg ctg gtc cag tac cac ctg tac ttc      385
Leu Gly Val Leu Ala Phe Phe Met Leu Val Gln Tyr His Leu Tyr Phe         
        115                 120                 125                     

att ggt gct ctc gtg ctc ggt atg cac tac cag caa atg gga tgg ctg      433
Ile Gly Ala Leu Val Leu Gly Met His Tyr Gln Gln Met Gly Trp Leu         
    130                 135                 140                         

tct cat gac atc tgc cac cac cag acc ttc aag aac cga aac tgg aat      481
Ser His Asp Ile Cys His His Gln Thr Phe Lys Asn Arg Asn Trp Asn         
145                 150                 155                 160         

aac gtc ctg ggt ctg gtc ttt ggc aac gga ctc cag ggc ttc tcc gtg      529
Asn Val Leu Gly Leu Val Phe Gly Asn Gly Leu Gln Gly Phe Ser Val         
                165                 170                 175             

acc tgg tgg aag gac aga cac aac gcc cat cat tct gct acc aac gtt      577
Thr Trp Trp Lys Asp Arg His Asn Ala His His Ser Ala Thr Asn Val         
            180                 185                 190                 

cag ggt cac gat ccc gac att gat aac ctg cct ctg ctc gcc tgg tcc      625
Gln Gly His Asp Pro Asp Ile Asp Asn Leu Pro Leu Leu Ala Trp Ser         
        195                 200                 205                     

gag gac gat gtc act cga gct tct ccc atc tcc cga aag ctc att cag      673
Glu Asp Asp Val Thr Arg Ala Ser Pro Ile Ser Arg Lys Leu Ile Gln         
    210                 215                 220                         

ttc caa cag tac tat ttc ctg gtc atc tgt att ctc ctg cga ttc atc      721
Phe Gln Gln Tyr Tyr Phe Leu Val Ile Cys Ile Leu Leu Arg Phe Ile         
225                 230                 235                 240         

tgg tgt ttc cag tct gtg ctg acc gtt cga tcc ctc aag gac cga gac      769
Trp Cys Phe Gln Ser Val Leu Thr Val Arg Ser Leu Lys Asp Arg Asp         
                245                 250                 255             

aac cag ttc tac cga tct cag tac aag aaa gag gcc att gga ctc gct      817
Asn Gln Phe Tyr Arg Ser Gln Tyr Lys Lys Glu Ala Ile Gly Leu Ala         
            260                 265                 270                 

ctg cac tgg act ctc aag acc ctg ttc cac ctc ttc ttt atg ccc tcc      865
Leu His Trp Thr Leu Lys Thr Leu Phe His Leu Phe Phe Met Pro Ser         
        275                 280                 285                     

atc ctg acc tcg atg ctg gtg ttc ttt gtt tcc gag ctc gtc ggt ggc      913
Ile Leu Thr Ser Met Leu Val Phe Phe Val Ser Glu Leu Val Gly Gly         
    290                 295                 300                         

ttc gga att gcc atc gtg gtc ttc atg aac cac tac cct ctg gag aag      961
Phe Gly Ile Ala Ile Val Val Phe Met Asn His Tyr Pro Leu Glu Lys         
305                 310                 315                 320         

atc ggt gat tcc gtc tgg gac gga cat ggc ttc tct gtg ggt cag atc     1009
Ile Gly Asp Ser Val Trp Asp Gly His Gly Phe Ser Val Gly Gln Ile         
                325                 330                 335             

cat gag acc atg aac att cga cga ggc atc att act gac tgg ttc ttt     1057
His Glu Thr Met Asn Ile Arg Arg Gly Ile Ile Thr Asp Trp Phe Phe         
            340                 345                 350                 

gga ggc ctg aac tac cag atc gag cac cat ctc tgg ccc acc ctg cct     1105
Gly Gly Leu Asn Tyr Gln Ile Glu His His Leu Trp Pro Thr Leu Pro         
        355                 360                 365                     

cga cac aac ctc act gcc gtt tcc tac cag gtg gaa cag ctg tgc cag     1153
Arg His Asn Leu Thr Ala Val Ser Tyr Gln Val Glu Gln Leu Cys Gln         
    370                 375                 380                         

aag cac aac ctc ccc tac cga aac cct ctg ccc cat gaa ggt ctc gtc     1201
Lys His Asn Leu Pro Tyr Arg Asn Pro Leu Pro His Glu Gly Leu Val         
385                 390                 395                 400         

atc ctg ctc cga tac ctg tcc cag ttc gct cga atg gcc gag aag cag     1249
Ile Leu Leu Arg Tyr Leu Ser Gln Phe Ala Arg Met Ala Glu Lys Gln         
                405                 410                 415             

ccc ggt gcc aag gct cag taa gc                                      1272
Pro Gly Ala Lys Ala Gln                                                 
            420                                                         


<210>  39
<211>  422
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  39

Met Val Lys Ala Ser Arg Gln Ala Leu Pro Leu Val Ile Asp Gly Lys 
1               5                   10                  15      


Val Tyr Asp Val Ser Ala Trp Val Asn Phe His Pro Gly Gly Ala Glu 
            20                  25                  30          


Ile Ile Glu Asn Tyr Gln Gly Arg Asp Ala Thr Asp Ala Phe Met Val 
        35                  40                  45              


Met His Ser Gln Glu Ala Phe Asp Lys Leu Lys Arg Met Pro Lys Ile 
    50                  55                  60                  


Asn Gln Ala Ser Glu Leu Pro Pro Gln Ala Ala Val Asn Glu Ala Gln 
65                  70                  75                  80  


Glu Asp Phe Arg Lys Leu Arg Glu Glu Leu Ile Ala Thr Gly Met Phe 
                85                  90                  95      


Asp Ala Ser Pro Leu Trp Tyr Ser Tyr Lys Ile Leu Thr Thr Leu Gly 
            100                 105                 110         


Leu Gly Val Leu Ala Phe Phe Met Leu Val Gln Tyr His Leu Tyr Phe 
        115                 120                 125             


Ile Gly Ala Leu Val Leu Gly Met His Tyr Gln Gln Met Gly Trp Leu 
    130                 135                 140                 


Ser His Asp Ile Cys His His Gln Thr Phe Lys Asn Arg Asn Trp Asn 
145                 150                 155                 160 


Asn Val Leu Gly Leu Val Phe Gly Asn Gly Leu Gln Gly Phe Ser Val 
                165                 170                 175     


Thr Trp Trp Lys Asp Arg His Asn Ala His His Ser Ala Thr Asn Val 
            180                 185                 190         


Gln Gly His Asp Pro Asp Ile Asp Asn Leu Pro Leu Leu Ala Trp Ser 
        195                 200                 205             


Glu Asp Asp Val Thr Arg Ala Ser Pro Ile Ser Arg Lys Leu Ile Gln 
    210                 215                 220                 


Phe Gln Gln Tyr Tyr Phe Leu Val Ile Cys Ile Leu Leu Arg Phe Ile 
225                 230                 235                 240 


Trp Cys Phe Gln Ser Val Leu Thr Val Arg Ser Leu Lys Asp Arg Asp 
                245                 250                 255     


Asn Gln Phe Tyr Arg Ser Gln Tyr Lys Lys Glu Ala Ile Gly Leu Ala 
            260                 265                 270         


Leu His Trp Thr Leu Lys Thr Leu Phe His Leu Phe Phe Met Pro Ser 
        275                 280                 285             


Ile Leu Thr Ser Met Leu Val Phe Phe Val Ser Glu Leu Val Gly Gly 
    290                 295                 300                 


Phe Gly Ile Ala Ile Val Val Phe Met Asn His Tyr Pro Leu Glu Lys 
305                 310                 315                 320 


Ile Gly Asp Ser Val Trp Asp Gly His Gly Phe Ser Val Gly Gln Ile 
                325                 330                 335     


His Glu Thr Met Asn Ile Arg Arg Gly Ile Ile Thr Asp Trp Phe Phe 
            340                 345                 350         


Gly Gly Leu Asn Tyr Gln Ile Glu His His Leu Trp Pro Thr Leu Pro 
        355                 360                 365             


Arg His Asn Leu Thr Ala Val Ser Tyr Gln Val Glu Gln Leu Cys Gln 
    370                 375                 380                 


Lys His Asn Leu Pro Tyr Arg Asn Pro Leu Pro His Glu Gly Leu Val 
385                 390                 395                 400 


Ile Leu Leu Arg Tyr Leu Ser Gln Phe Ala Arg Met Ala Glu Lys Gln 
                405                 410                 415     


Pro Gly Ala Lys Ala Gln 
            420         


<210>  40
<211>  777
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(777)
<223>  delta-9 elongase

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  WO 2007/061742
<311>  2006-11-16
<312>  2007-05-31
<313>  (1)..(777)

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  US 2007-0117190-A1
<311>  2006-11-16
<312>  2007-05-24
<313>  (1)..(777)

<400>  40
atg gag gtg gtg aat gaa ata gtc tca att ggg cag gaa gtt tta ccc       48
Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro         
1               5                   10                  15              

aaa gtt gat tat gcc caa ctc tgg agt gat gcc agt cac tgt gag gtg       96
Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val         
            20                  25                  30                  

ctt tac ttg tcc atc gca ttt gtc atc ttg aag ttc act ctt ggc ccc      144
Leu Tyr Leu Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro         
        35                  40                  45                      

ctt ggt cca aaa ggt cag tct cgt atg aag ttt gtt ttc acc aat tac      192
Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr         
    50                  55                  60                          

aac ctt ctc atg tcc att tat tcg ttg gga tca ttc ctc tca atg gca      240
Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala         
65                  70                  75                  80          

tat gcc atg tac acc atc ggt gtt atg tct gac aac tgc gag aag gct      288
Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala         
                85                  90                  95              

ttt gac aac aac gtc ttc agg atc acc acg cag ttg ttc tat ttg agc      336
Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser         
            100                 105                 110                 

aag ttc ctg gag tat att gac tcc ttc tat ttg cca ctg atg ggc aag      384
Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys         
        115                 120                 125                     

cct ctg acc tgg ttg caa ttc ttc cat cat ttg ggg gca ccg atg gat      432
Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp         
    130                 135                 140                         

atg tgg ctg ttc tat aat tac cga aat gaa gct gtt tgg att ttt gtg      480
Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val         
145                 150                 155                 160         

ctg ttg aat ggt ttc atc cac tgg atc atg tac ggt tat tat tgg acc      528
Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr         
                165                 170                 175             

aga ttg atc aag ctg aag ttc ccc atg cca aaa tcc ctg att aca tca      576
Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser         
            180                 185                 190                 

atg cag atc att caa ttc aat gtt ggt ttc tac att gtc tgg aag tac      624
Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr         
        195                 200                 205                     

agg aac att ccc tgt tat cgc caa gat ggg atg agg atg ttt ggc tgg      672
Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp         
    210                 215                 220                         

ttc ttc aat tac ttt tat gtt ggc aca gtc ttg tgt ttg ttc ttg aat      720
Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn         
225                 230                 235                 240         

ttc tat gtg caa acg tat atc gtc agg aag cac aag gga gcc aaa aag      768
Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys         
                245                 250                 255             

att cag tga                                                          777
Ile Gln                                                                 
                                                                        


<210>  41
<211>  258
<212>  PRT
<213>  Euglena gracilis

<400>  41

Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro 
1               5                   10                  15      


Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val 
            20                  25                  30          


Leu Tyr Leu Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro 
        35                  40                  45              


Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr 
    50                  55                  60                  


Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala 
65                  70                  75                  80  


Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala 
                85                  90                  95      


Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser 
            100                 105                 110         


Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys 
        115                 120                 125             


Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp 
    130                 135                 140                 


Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val 
145                 150                 155                 160 


Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr 
                165                 170                 175     


Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 
        195                 200                 205             


Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp 
    210                 215                 220                 


Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys 
                245                 250                 255     


Ile Gln 
        


<210>  42
<211>  13707
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZKSL-555R

<400>  42
aaacagtgta cgcagatctg cccatgatgg gggctcccac caccagcaat cagggccctg     60

attacacacc cacctgtaat gtcatgctgt tcatcgtggt taatgctgct gtgtgctgtg    120

tgtgtgtgtt gtttggcgct cattgttgcg ttatgcagcg tacaccacaa tattggaagc    180

ttattagcct ttctattttt tcgtttgcaa ggcttaacaa cattgctgtg gagagggatg    240

gggatatgga ggccgctgga gggagtcgga gaggcgtttt ggagcggctt ggcctggcgc    300

ccagctcgcg aaacgcacct aggacccttt ggcacgccga aatgtgccac ttttcagtct    360

agtaacgcct tacctacgtc attccatgcg tgcatgtttg cgcctttttt cccttgccct    420

tgatcgccac acagtacagt gcactgtaca gtggaggttt tgggggggtc ttagatggga    480

gctaaaagcg gcctagcggt acactagtgg gattgtatgg agtggcatgg agcctaggtg    540

gagcctgaca ggacgcacga ccggctagcc cgtgacagac gatgggtggc tcctgttgtc    600

caccgcgtac aaatgtttgg gccaaagtct tgtcagcctt gcttgcgaac ctaattccca    660

attttgtcac ttcgcacccc cattgatcga gccctaaccc ctgcccatca ggcaatccaa    720

ttaagctcgc attgtctgcc ttgtttagtt tggctcctgc ccgtttcggc gtccacttgc    780

acaaacacaa acaagcatta tatataaggc tcgtctctcc ctcccaacca cactcacttt    840

tttgcccgtc ttcccttgct aacacaaaag tcaagaacac aaacaaccac cccaaccccc    900

ttacacacaa gacatatcta cagcaatggc catggctctc tcccttacta ccgagcagct    960

gctcgagcga cccgacctgg ttgccatcga cggcattctc tacgatctgg aaggtcttgc   1020

caaggtccat cccggaggcg acttgatcct cgcttctggt gcctccgatg cttctcctct   1080

gttctactcc atgcaccctt acgtcaagcc cgagaactcg aagctgcttc aacagttcgt   1140

gcgaggcaag cacgaccgaa cctccaagga cattgtctac acctacgact ctccctttgc   1200

acaggacgtc aagcgaacta tgcgagaggt catgaaaggt cggaactggt atgccacacc   1260

tggattctgg ctgcgaaccg ttggcatcat tgctgtcacc gccttttgcg agtggcactg   1320

ggctactacc ggaatggtgc tgtggggtct cttgactgga ttcatgcaca tgcagatcgg   1380

cctgtccatt cagcacgatg cctctcatgg tgccatcagc aaaaagccct gggtcaacgc   1440

tctctttgcc tacggcatcg acgtcattgg atcgtccaga tggatctggc tgcagtctca   1500

catcatgcga catcacacct acaccaatca gcatggtctc gacctggatg ccgagtccgc   1560

agaaccattc cttgtgttcc acaactaccc tgctgccaac actgctcgaa agtggtttca   1620

ccgattccag gcctggtaca tgtacctcgt gcttggagcc tacggcgttt cgctggtgta   1680

caaccctctc tacatcttcc gaatgcagca caacgacacc attcccgagt ctgtcacagc   1740

catgcgagag aacggctttc tgcgacggta ccgaaccctt gcattcgtta tgcgagcttt   1800

cttcatcttt cgaaccgcct tcttgccctg gtatctcact ggaacctccc tgctcatcac   1860

cattcctctg gtgcccactg ctaccggtgc cttcctcacc ttctttttca tcttgtctca   1920

caacttcgat ggctcggagc gaatccccga caagaactgc aaggtcaaga gctccgagaa   1980

ggacgttgaa gccgatcaga tcgactggta cagagctcag gtggagacct cttccaccta   2040

cggtggaccc attgccatgt tctttactgg cggtctcaac ttccagatcg agcatcacct   2100

ctttcctcga atgtcgtctt ggcactatcc cttcgtgcag caagctgtcc gagagtgttg   2160

cgaacgacac ggagttcggt acgtcttcta ccctaccatt gtgggcaaca tcatttccac   2220

cctcaagtac atgcacaaag tcggtgtggt tcactgtgtc aaggacgctc aggattccta   2280

agcggccgca agtgtggatg gggaagtgag tgcccggttc tgtgtgcaca attggcaatc   2340

caagatggat ggattcaaca cagggatata gcgagctacg tggtggtgcg aggatatagc   2400

aacggatatt tatgtttgac acttgagaat gtacgataca agcactgtcc aagtacaata   2460

ctaaacatac tgtacatact catactcgta cccgggcaac ggtttcactt gagtgcagtg   2520

gctagtgctc ttactcgtac agtgtgcaat actgcgtatc atagtctttg atgtatatcg   2580

tattcattca tgttagttgc gtacgctgtg ttgttgtatg tggtgaagct tgacaatgga   2640

tggtgtgtcg tatcaggctg gggaacaatt gtgcttaagt atgctgcagt tgagtaagag   2700

tcatcgctcc accaaaataa agtttgccat tagggttgga gagagagatg gtggctggaa   2760

gaattaaatg acatcaagct gaggattgtg ggtgtgcaat aacacatgtt aggggtgacc   2820

tgtggctcga aatctgataa ttattttgta actttatgat tattcttaga ttttttaata   2880

ttcctctata taacacataa gtagctgtcg tctagttgtt catagcctga ctcctgcaat   2940

agattagtgc agagtgattt tgtgcaattg agagccacgg ttgagtcaag tgactttgtg   3000

tgtgaagtca tcttacgttt caagtctcac aggttactca attggttggt tgtctgccct   3060

ttacagatat ttacagtacc tgagcgtaaa gtcgttcatc cacggaatga ctgttcctgt   3120

cacgcagtca tgatcatgga tgtggctggt caggaaccat tttggatagg agacttaggg   3180

attggactat tattgaaaaa actgagccga atatgatata gttctatttg aatgcagaac   3240

ttctgatggt caattcactt atttcaggca tatcggtcat ggtggcagct gccacgatgt   3300

tatctcgttg gaaacctcgg cgcgccagct gcattaatga atcggccaac gcgcggggag   3360

aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt   3420

cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga   3480

atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg   3540

taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa   3600

aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt   3660

tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct   3720

gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct   3780

cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc   3840

cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt   3900

atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc   3960

tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat   4020

ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa   4080

acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa   4140

aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga   4200

aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct   4260

tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga   4320

cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc   4380

catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg   4440

ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat   4500

aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat   4560

ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg   4620

caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc   4680

attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa   4740

agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc   4800

actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt   4860

ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag   4920

ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt   4980

gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag   5040

atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac   5100

cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc   5160

gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca   5220

gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg   5280

ggttccgcgc acatttcccc gaaaagtgcc acctgatgcg gtgtgaaata ccgcacagat   5340

gcgtaaggag aaaataccgc atcaggaaat tgtaagcgtt aatattttgt taaaattcgc   5400

gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc   5460

ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag   5520

tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga   5580

tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc   5640

actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa   5700

cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt   5760

agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc   5820

gtccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg   5880

ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca   5940

gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta   6000

tagggcgaat tgggcccgac gtcgcatgca ttccatagcc acacctttgc ctatggcttc   6060

acaaccgaag gcaattcgag aggtcgcgct tatggaatcg actcgtataa agctgaaggg   6120

aaagggagac gttccgagcg ctcagatgca atagtcgtcc agctaatgtg gattcaaaaa   6180

caaccccaac agtaatcttg aaaatttgaa cggatcaatc tgaacactct tgctccaggt   6240

cattcttcta acgcacatcc ccagagtcta gagggagttg tgttgtgaac atcctaataa   6300

acaatgcaat ggattcggga tatcttctgt ctcgccccct actcgatgtc gagtaaaccg   6360

atcaccaact aacaatactc ctccgcgttc tgccattgac tctcaaacag acatcgctat   6420

caacggaaca gcatatttta gcttcttagg acaataaata ttgataatgc cggctctccc   6480

tcggtatatt aagcaatcca ttcatacact cattcatcag gttaatttta tatatataat   6540

ttgtctattc aaacaccgta aattactggt accatcatct cctccttttc aaatacacgt   6600

ctatttgcat taatgaaatt actcgccaat tcgcagaacg tgtttgtcga acagagcctt   6660

agctcgggtc cagacaggag cagtgtctcg ctgaggaagc tgcaggagag ttaattaact   6720

cacctgcagg attgagacta tgaatggatt cccgtgcccg tattactcta ctaatttgat   6780

cttggaacgc gaaaatacgt ttctaggact ccaaagaatc tcaactcttg tccttactaa   6840

atatactacc catagttgat ggtttacttg aacagagagg acatgttcac ttgacccaaa   6900

gtttctcgca tctcttggat atttgaacaa cggcgtccac tgaccgtcag ttatccagtc   6960

acaaaacccc cacattcata cattcccatg tacgtttaca aagttctcaa ttccatcgtg   7020

caaatcaaaa tcacatctat tcattcatca tatataaacc catcatgtct actaacactc   7080

acaactccat agaaaacatc gactcagaac acacgctcca tgcggccgct taggaatcct   7140

gtgcgtcctt cacgcagtgg acgacaccca ccttatgcat gtacttcagg gtggagatga   7200

tgttgccgac gatggtaggg tagaaaacat atcgcactcc atgtcgttcg caacactccc   7260

ggaccgcctg ctggacgaag gggtagtgcc aagacgacat ccggggaaag aggtggtgct   7320

cgatctggaa attgagaccg ccagtgaaga acatggcgat ggggccaccg tatgtggagg   7380

acgtctccac ctgcgcccga taccagtcaa tttggtcagc ctcaacgtcc ttctcagatc   7440

gcttaacctt gcagttcttg tcggggatcc gttcggagcc atcaaaattg tgggacaaaa   7500

tgaagaagaa cgtcaagaag gcaccagttg cggtgggcac cagaggaatg gtgatcagca   7560

atgaggtccc agtgaggtac cagggcaaga atgcggtccg gaagatgaag aaagctcgca   7620

tcacgaatgc aagtgtgcgg tagcgccgca gaaagccatt ttcccgcatg gccgtgacag   7680

actctgggat ggtgtcattg tgctgcatcc ggaaaatgta gagcgggttg tacaccagcg   7740

ataccccgta tgcccccagc acaaggtaca tgtaccaagc ctggaagcgg tggaaccact   7800

ttcgggcggt gtttgcggcg gggtagttgt ggaacaccag gaacggctct gccgactccg   7860

catccaggtc gaggccgtgc tggttggtgt aggtgtggtg ccgcatgatg tgcgactgca   7920

gccaaatcca ccgggacgat ccgatgacgt caatgccgta ggcgaagagg gcgttgaccc   7980

aaggcttctt gctgatggcc ccgtgggacg catcatgctg gatggataag ccgatctgca   8040

tgtgcatgaa tccagtcaac aggccccaca gcaccatccc cgtggtagcc cagtgccact   8100

cgcaaaaggc cgtcacggcg atgatcccaa cggtgcgcag ccagaagcca ggggttgcgt   8160

accagttcct ccctttcatc acctcgcgca ttgtccgctt aacgtcttgt gcgaagggag   8220

aatcatacgt gtagacaatg tccttcgagg tgcggtcatg cttccctcgg acgaactgtt   8280

gaagcaattt ggagttctcc ggtttgacgt atggatgcat tgaataaaag agaggggagg   8340

catcagaggc accagaagcg agaatcaaat ctcctcctgg atgaactttg gcaagccctt   8400

caaggtcgta gaggatgcca tcaatcgcaa ccaaatcagg gcgttctaac agctgttctg   8460

tggtaagact gagagccatg gagagctggg ttagtttgtg tagagagtgt gtgttgctag   8520

cgactttcgg attgtgtcat tacacaaaac gcgtcgtctc gacactgatc ttgtcgtgga   8580

tactcacggc tcggacatcg tcgccgacga tgacaccgga ctttcgctta aggacgtcag   8640

taacaggcat tgtgtgatgt gtagtttaga tttcgaatct gtggggaaag aaaggaaaaa   8700

agagactggc aaccgattgg gagagccact gtttatatat accctagaca agccccccgc   8760

ttgtaagatg ttggtcaatg taaaccagta ttaaggttgg caagtgcagg agaagcaagg   8820

tgtgggtacc gagcaatgga aatgtgcgga aggcaaaaaa atgaggccac ggcctattgt   8880

cggggctata tccagggggc gattgaagta cactaacatg acatgtgtcc acagaccctc   8940

aatctggcct gatgagccaa atccatacgc gctttcgcag ctctaaaggc tataacaagt   9000

cacaccaccc tgctcgacct cagcgccctc actttttgtt aagacaaact gtacacgctg   9060

ttccagcgtt ttctgcctgc acctggtggg acatttggtg caacctaaag tgctcggaac   9120

ctctgtggtg tccagatcag cgcagcagtt ccgaggtagt tttgaggccc ttagatgatg   9180

caatggtgtc agtcgctgga tcacgagtct taatggcagt attcgttctt atttgtgcca   9240

ttgagccccg ttatcctcgt atcttctacc ccccatccca tccctttgtt ggtgcaaccc   9300

tacccattta ttgttgggtg cagcccaacc gacgtggaga gcttggcttg gccatataaa   9360

aaggcccccc cctagtggca atggcagaaa gtcagctgtg agttgttgaa tttgtcatct   9420

aggcggcctg gccgtcttct ccggggcaat tggggctgtt ttttgggaca caaatacgcc   9480

gccaacccgg tctctcctga attccgtcgt cgcctgagtc gacatcattt atttaccagt   9540

tggccacaaa cccttgacga tctcgtatgt cccctccgac atactcccgg ccggctgggg   9600

tacgttcgat agcgctatcg gcatcgacaa ggtttgggtc cctagccgat accgcactac   9660

ctgagtcaca atcttcggag gtttagtctt ccacatagca cgggcaaaag tgcgtatata   9720

tacaagagcg tttgccagcc acagattttc actccacaca ccacatcaca catacaacca   9780

cacacatcca caatggaacc cgaaactaag aagaccaaga ctgactccaa gaagattgtt   9840

cttctcggcg gcgacttctg tggccccgag gtgattgccg aggccgtcaa ggtgctcaag   9900

tctgttgctg aggcctccgg caccgagttt gtgtttgagg accgactcat tggaggagct   9960

gccattgaga aggagggcga gcccatcacc gacgctactc tcgacatctg ccgaaaggct  10020

gactctatta tgctcggtgc tgtcggaggc gctgccaaca ccgtatggac cactcccgac  10080

ggacgaaccg acgtgcgacc cgagcagggt ctcctcaagc tgcgaaagga cctgaacctg  10140

tacgccaacc tgcgaccctg ccagctgctg tcgcccaagc tcgccgatct ctcccccatc  10200

cgaaacgttg agggcaccga cttcatcatt gtccgagagc tcgtcggagg tatctacttt  10260

ggagagcgaa aggaggatga cggatctggc gtcgcttccg acaccgagac ctactccgtt  10320

cctgaggttg agcgaattgc ccgaatggcc gccttcctgg cccttcagca caacccccct  10380

cttcccgtgt ggtctcttga caaggccaac gtgctggcct cctctcgact ttggcgaaag  10440

actgtcactc gagtcctcaa ggacgaactc ccccagctcg agctcaacca ccagctgatc  10500

gactcggccg ccatgatcct catcaagcag ccctccaaga tgaatggtat catcatcacc  10560

accaacatgt ttggcgatat catctccgac gaggcctccg tcatccccgg ttctctgggt  10620

ctgctgccct ccgcctctct ggcttctctg cccgacacca acgaggcgtt cggtctgtac  10680

gagccctgtc acggatctgc ccccgatctc ggcaagcaga aggtcaaccc cattgccacc  10740

attctgtctg ccgccatgat gctcaagttc tctcttaaca tgaagcccgc cggtgacgct  10800

gttgaggctg ccgtcaagga gtccgtcgag gctggtatca ctaccgccga tatcggaggc  10860

tcttcctcca cctccgaggt cggagacttg ttgccaacaa ggtcaaggag ctgctcaaga  10920

aggagtaagt cgtttctacg acgcattgat ggaaggagca aactgacgcg cctgcgggtt  10980

ggtctaccgg cagggtccgc tagtgtataa gactctataa aaagggccct gccctgctaa  11040

tgaaatgatg atttataatt taccggtgta gcaaccttga ctagaagaag cagattgggt  11100

gtgtttgtag tggaggacag tggtacgttt tggaaacagt cttcttgaaa gtgtcttgtc  11160

tacagtatat tcactcataa cctcaatagc caagggtgta gtcggtttat taaaggaagg  11220

gagttgtggc tgatgtggat atcgatagtt ggagcaaggg agaaatgtag agtgtgaaag  11280

actcactatg gtccgggctt atctcgacca atagccaaag tctggagttt ctgagagaaa  11340

aaggcaagat acgtatgtaa caaagcgacg catggtacaa taataccgga ggcatgtatc  11400

atagagagtt agtggttcga tgatggcact ggtgcctggt atgactttat acggctgact  11460

acatatttgt cctcagacat acaattacag tcaagcactt acccttggac atctgtaggt  11520

accccccggc caagacgatc tcagcgtgtc gtatgtcgga ttggcgtagc tccctcgctc  11580

gtcaattggc tcccatctac tttcttctgc ttggctacac ccagcatgtc tgctatggct  11640

cgttttcgtg ccttatctat cctcccagta ttaccaactc taaatgacat gatgtgattg  11700

ggtctacact ttcatatcag agataaggag tagcacagtt gcataaaaag cccaactcta  11760

atcagcttct tcctttcttg taattagtac aaaggtgatt agcgaaatct ggaagcttag  11820

ttggccctaa aaaaatcaaa aaaagcaaaa aacgaaaaac gaaaaaccac agttttgaga  11880

acagggaggt aacgaaggat cgtatatata tatatatata tatataccca cggatcccga  11940

gaccggcctt tgattcttcc ctacaaccaa ccattctcac caccctaatt cacaaccatg  12000

gctcccgacg ccgacaagct gcgacagcga aaggctcagt ccatccagga cactgccgat  12060

tctcaggcta ccgagctcaa gattggcacc ctgaagggtc tccaaggcac cgagatcgtc  12120

attgatggcg acatctacga catcaaagac ttcgatcacc ctggaggcga atccatcatg  12180

acctttggtg gcaacgacgt tactgccacc tacaagatga ttcatcccta ccactcgaag  12240

catcacctgg agaagatgaa aaaggtcggt cgagtgcccg actacacctc cgagtacaag  12300

ttcgatactc ccttcgaacg agagatcaaa caggaggtct tcaagattgt gcgaagaggt  12360

cgagagtttg gaacacctgg ctacttcttt cgagccttct gctacatcgg tctcttcttt  12420

tacctgcagt atctctgggt taccactcct accactttcg cccttgctat cttctacggt  12480

gtgtctcagg ccttcattgg cctgaacgtc cagcacgacg ccaaccacgg agctgcctcc  12540

aaaaagccct ggatcaacaa tttgctcggc ctgggtgccg actttatcgg aggctccaag  12600

tggctctgga tgaaccagca ctggacccat cacacttaca ccaaccatca cgagaaggat  12660

cccgacgccc tgggtgcaga gcctatgctg ctcttcaacg actatccctt gggtcacccc  12720

aagcgaaccc tcattcatca cttccaagcc ttctactatc tgtttgtcct tgctggctac  12780

tgggtgtctt cggtgttcaa ccctcagatc ctggacctcc agcaccgagg tgcccaggct  12840

gtcggcatga agatggagaa cgactacatt gccaagtctc gaaagtacgc tatcttcctg  12900

cgactcctgt acatctacac caacattgtg gctcccatcc agaaccaagg cttttcgctc  12960

accgtcgttg ctcacattct tactatgggt gtcgcctcca gcctgaccct cgctactctg  13020

ttcgccctct cccacaactt cgagaacgca gatcgggatc ccacctacga ggctcgaaag  13080

ggaggcgagc ctgtctgttg gttcaagtcg caggtggaaa cctcctctac ttacggtggc  13140

ttcatttccg gttgccttac aggcggactc aactttcagg tcgagcatca cctgtttcct  13200

cgaatgtcct ctgcctggta cccctacatc gctcctaccg ttcgagaggt ctgcaaaaag  13260

cacggcgtca agtacgccta ctatccctgg gtgtggcaga acctcatctc gaccgtcaag  13320

tacctgcatc agtccggaac tggctcgaac tggaagaacg gtgccaatcc ctactctggc  13380

aagctgtaag cggccgcatg tacatacaag attatttata gaaatgaatc gcgatcgaac  13440

aaagagtacg agtgtacgag taggggatga tgataaaagt ggaagaagtt ccgcatcttt  13500

ggatttatca acgtgtagga cgatacttcc tgtaaaaatg caatgtcttt accataggtt  13560

ctgctgtaga tgttattaac taccattaac atgtctactt gtacagttgc agaccagttg  13620

gagtatagaa tggtacactt accaaaaagt gttgatggtt gtaactacga tatataaaac  13680

tgttgacggg atctgcgtac actgttt                                      13707


<210>  43
<211>  1350
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(1350)
<223>  synthetic delta-5 desaturase (codon-optimized for Yarrowia 
       lipolytica)

<400>  43
atg gct ctc tcc ctt act acc gag cag ctg ctc gag cga ccc gac ctg       48
Met Ala Leu Ser Leu Thr Thr Glu Gln Leu Leu Glu Arg Pro Asp Leu         
1               5                   10                  15              

gtt gcc atc gac ggc att ctc tac gat ctg gaa ggt ctt gcc aag gtc       96
Val Ala Ile Asp Gly Ile Leu Tyr Asp Leu Glu Gly Leu Ala Lys Val         
            20                  25                  30                  

cat ccc gga ggc gac ttg atc ctc gct tct ggt gcc tcc gat gct tct      144
His Pro Gly Gly Asp Leu Ile Leu Ala Ser Gly Ala Ser Asp Ala Ser         
        35                  40                  45                      

cct ctg ttc tac tcc atg cac cct tac gtc aag ccc gag aac tcg aag      192
Pro Leu Phe Tyr Ser Met His Pro Tyr Val Lys Pro Glu Asn Ser Lys         
    50                  55                  60                          

ctg ctt caa cag ttc gtg cga ggc aag cac gac cga acc tcc aag gac      240
Leu Leu Gln Gln Phe Val Arg Gly Lys His Asp Arg Thr Ser Lys Asp         
65                  70                  75                  80          

att gtc tac acc tac gac tct ccc ttt gca cag gac gtc aag cga act      288
Ile Val Tyr Thr Tyr Asp Ser Pro Phe Ala Gln Asp Val Lys Arg Thr         
                85                  90                  95              

atg cga gag gtc atg aaa ggt cgg aac tgg tat gcc aca cct gga ttc      336
Met Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly Phe         
            100                 105                 110                 

tgg ctg cga acc gtt ggc atc att gct gtc acc gcc ttt tgc gag tgg      384
Trp Leu Arg Thr Val Gly Ile Ile Ala Val Thr Ala Phe Cys Glu Trp         
        115                 120                 125                     

cac tgg gct act acc gga atg gtg ctg tgg ggt ctc ttg act gga ttc      432
His Trp Ala Thr Thr Gly Met Val Leu Trp Gly Leu Leu Thr Gly Phe         
    130                 135                 140                         

atg cac atg cag atc ggc ctg tcc att cag cac gat gcc tct cat ggt      480
Met His Met Gln Ile Gly Leu Ser Ile Gln His Asp Ala Ser His Gly         
145                 150                 155                 160         

gcc atc agc aaa aag ccc tgg gtc aac gct ctc ttt gcc tac ggc atc      528
Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Leu Phe Ala Tyr Gly Ile         
                165                 170                 175             

gac gtc att gga tcg tcc aga tgg atc tgg ctg cag tct cac atc atg      576
Asp Val Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile Met         
            180                 185                 190                 

cga cat cac acc tac acc aat cag cat ggt ctc gac ctg gat gcc gag      624
Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala Glu         
        195                 200                 205                     

tcc gca gaa cca ttc ctt gtg ttc cac aac tac cct gct gcc aac act      672
Ser Ala Glu Pro Phe Leu Val Phe His Asn Tyr Pro Ala Ala Asn Thr         
    210                 215                 220                         

gct cga aag tgg ttt cac cga ttc cag gcc tgg tac atg tac ctc gtg      720
Ala Arg Lys Trp Phe His Arg Phe Gln Ala Trp Tyr Met Tyr Leu Val         
225                 230                 235                 240         

ctt gga gcc tac ggc gtt tcg ctg gtg tac aac cct ctc tac atc ttc      768
Leu Gly Ala Tyr Gly Val Ser Leu Val Tyr Asn Pro Leu Tyr Ile Phe         
                245                 250                 255             

cga atg cag cac aac gac acc att ccc gag tct gtc aca gcc atg cga      816
Arg Met Gln His Asn Asp Thr Ile Pro Glu Ser Val Thr Ala Met Arg         
            260                 265                 270                 

gag aac ggc ttt ctg cga cgg tac cga acc ctt gca ttc gtt atg cga      864
Glu Asn Gly Phe Leu Arg Arg Tyr Arg Thr Leu Ala Phe Val Met Arg         
        275                 280                 285                     

gct ttc ttc atc ttt cga acc gcc ttc ttg ccc tgg tat ctc act gga      912
Ala Phe Phe Ile Phe Arg Thr Ala Phe Leu Pro Trp Tyr Leu Thr Gly         
    290                 295                 300                         

acc tcc ctg ctc atc acc att cct ctg gtg ccc act gct acc ggt gcc      960
Thr Ser Leu Leu Ile Thr Ile Pro Leu Val Pro Thr Ala Thr Gly Ala         
305                 310                 315                 320         

ttc ctc acc ttc ttt ttc atc ttg tct cac aac ttc gat ggc tcg gag     1008
Phe Leu Thr Phe Phe Phe Ile Leu Ser His Asn Phe Asp Gly Ser Glu         
                325                 330                 335             

cga atc ccc gac aag aac tgc aag gtc aag agc tcc gag aag gac gtt     1056
Arg Ile Pro Asp Lys Asn Cys Lys Val Lys Ser Ser Glu Lys Asp Val         
            340                 345                 350                 

gaa gcc gat cag atc gac tgg tac aga gct cag gtg gag acc tct tcc     1104
Glu Ala Asp Gln Ile Asp Trp Tyr Arg Ala Gln Val Glu Thr Ser Ser         
        355                 360                 365                     

acc tac ggt gga ccc att gcc atg ttc ttt act ggc ggt ctc aac ttc     1152
Thr Tyr Gly Gly Pro Ile Ala Met Phe Phe Thr Gly Gly Leu Asn Phe         
    370                 375                 380                         

cag atc gag cat cac ctc ttt cct cga atg tcg tct tgg cac tat ccc     1200
Gln Ile Glu His His Leu Phe Pro Arg Met Ser Ser Trp His Tyr Pro         
385                 390                 395                 400         

ttc gtg cag caa gct gtc cga gag tgt tgc gaa cga cac gga gtt cgg     1248
Phe Val Gln Gln Ala Val Arg Glu Cys Cys Glu Arg His Gly Val Arg         
                405                 410                 415             

tac gtc ttc tac cct acc att gtg ggc aac atc att tcc acc ctc aag     1296
Tyr Val Phe Tyr Pro Thr Ile Val Gly Asn Ile Ile Ser Thr Leu Lys         
            420                 425                 430                 

tac atg cac aaa gtc ggt gtg gtt cac tgt gtc aag gac gct cag gat     1344
Tyr Met His Lys Val Gly Val Val His Cys Val Lys Asp Ala Gln Asp         
        435                 440                 445                     

tcc taa                                                             1350
Ser                                                                     
                                                                        


<210>  44
<211>  449
<212>  PRT
<213>  Euglena gracilis

<400>  44

Met Ala Leu Ser Leu Thr Thr Glu Gln Leu Leu Glu Arg Pro Asp Leu 
1               5                   10                  15      


Val Ala Ile Asp Gly Ile Leu Tyr Asp Leu Glu Gly Leu Ala Lys Val 
            20                  25                  30          


His Pro Gly Gly Asp Leu Ile Leu Ala Ser Gly Ala Ser Asp Ala Ser 
        35                  40                  45              


Pro Leu Phe Tyr Ser Met His Pro Tyr Val Lys Pro Glu Asn Ser Lys 
    50                  55                  60                  


Leu Leu Gln Gln Phe Val Arg Gly Lys His Asp Arg Thr Ser Lys Asp 
65                  70                  75                  80  


Ile Val Tyr Thr Tyr Asp Ser Pro Phe Ala Gln Asp Val Lys Arg Thr 
                85                  90                  95      


Met Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly Phe 
            100                 105                 110         


Trp Leu Arg Thr Val Gly Ile Ile Ala Val Thr Ala Phe Cys Glu Trp 
        115                 120                 125             


His Trp Ala Thr Thr Gly Met Val Leu Trp Gly Leu Leu Thr Gly Phe 
    130                 135                 140                 


Met His Met Gln Ile Gly Leu Ser Ile Gln His Asp Ala Ser His Gly 
145                 150                 155                 160 


Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Leu Phe Ala Tyr Gly Ile 
                165                 170                 175     


Asp Val Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile Met 
            180                 185                 190         


Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala Glu 
        195                 200                 205             


Ser Ala Glu Pro Phe Leu Val Phe His Asn Tyr Pro Ala Ala Asn Thr 
    210                 215                 220                 


Ala Arg Lys Trp Phe His Arg Phe Gln Ala Trp Tyr Met Tyr Leu Val 
225                 230                 235                 240 


Leu Gly Ala Tyr Gly Val Ser Leu Val Tyr Asn Pro Leu Tyr Ile Phe 
                245                 250                 255     


Arg Met Gln His Asn Asp Thr Ile Pro Glu Ser Val Thr Ala Met Arg 
            260                 265                 270         


Glu Asn Gly Phe Leu Arg Arg Tyr Arg Thr Leu Ala Phe Val Met Arg 
        275                 280                 285             


Ala Phe Phe Ile Phe Arg Thr Ala Phe Leu Pro Trp Tyr Leu Thr Gly 
    290                 295                 300                 


Thr Ser Leu Leu Ile Thr Ile Pro Leu Val Pro Thr Ala Thr Gly Ala 
305                 310                 315                 320 


Phe Leu Thr Phe Phe Phe Ile Leu Ser His Asn Phe Asp Gly Ser Glu 
                325                 330                 335     


Arg Ile Pro Asp Lys Asn Cys Lys Val Lys Ser Ser Glu Lys Asp Val 
            340                 345                 350         


Glu Ala Asp Gln Ile Asp Trp Tyr Arg Ala Gln Val Glu Thr Ser Ser 
        355                 360                 365             


Thr Tyr Gly Gly Pro Ile Ala Met Phe Phe Thr Gly Gly Leu Asn Phe 
    370                 375                 380                 


Gln Ile Glu His His Leu Phe Pro Arg Met Ser Ser Trp His Tyr Pro 
385                 390                 395                 400 


Phe Val Gln Gln Ala Val Arg Glu Cys Cys Glu Arg His Gly Val Arg 
                405                 410                 415     


Tyr Val Phe Tyr Pro Thr Ile Val Gly Asn Ile Ile Ser Thr Leu Lys 
            420                 425                 430         


Tyr Met His Lys Val Gly Val Val His Cys Val Lys Asp Ala Gln Asp 
        435                 440                 445             


Ser 
    


<210>  45
<211>  1392
<212>  DNA
<213>  Peridinium sp. CCMP626


<220>
<221>  CDS
<222>  (1)..(1392)
<223>  synthetic delta-5 desaturase (codon-optimized for Yarrowia 
       lipolytica)

<400>  45
atg gct ccc gac gcc gac aag ctg cga cag cga aag gct cag tcc atc       48
Met Ala Pro Asp Ala Asp Lys Leu Arg Gln Arg Lys Ala Gln Ser Ile         
1               5                   10                  15              

cag gac act gcc gat tct cag gct acc gag ctc aag att ggc acc ctg       96
Gln Asp Thr Ala Asp Ser Gln Ala Thr Glu Leu Lys Ile Gly Thr Leu         
            20                  25                  30                  

aag ggt ctc caa ggc acc gag atc gtc att gat ggc gac atc tac gac      144
Lys Gly Leu Gln Gly Thr Glu Ile Val Ile Asp Gly Asp Ile Tyr Asp         
        35                  40                  45                      

atc aaa gac ttc gat cac cct gga ggc gaa tcc atc atg acc ttt ggt      192
Ile Lys Asp Phe Asp His Pro Gly Gly Glu Ser Ile Met Thr Phe Gly         
    50                  55                  60                          

ggc aac gac gtt act gcc acc tac aag atg att cat ccc tac cac tcg      240
Gly Asn Asp Val Thr Ala Thr Tyr Lys Met Ile His Pro Tyr His Ser         
65                  70                  75                  80          

aag cat cac ctg gag aag atg aaa aag gtc ggt cga gtg ccc gac tac      288
Lys His His Leu Glu Lys Met Lys Lys Val Gly Arg Val Pro Asp Tyr         
                85                  90                  95              

acc tcc gag tac aag ttc gat act ccc ttc gaa cga gag atc aaa cag      336
Thr Ser Glu Tyr Lys Phe Asp Thr Pro Phe Glu Arg Glu Ile Lys Gln         
            100                 105                 110                 

gag gtc ttc aag att gtg cga aga ggt cga gag ttt gga aca cct ggc      384
Glu Val Phe Lys Ile Val Arg Arg Gly Arg Glu Phe Gly Thr Pro Gly         
        115                 120                 125                     

tac ttc ttt cga gcc ttc tgc tac atc ggt ctc ttc ttt tac ctg cag      432
Tyr Phe Phe Arg Ala Phe Cys Tyr Ile Gly Leu Phe Phe Tyr Leu Gln         
    130                 135                 140                         

tat ctc tgg gtt acc act cct acc act ttc gcc ctt gct atc ttc tac      480
Tyr Leu Trp Val Thr Thr Pro Thr Thr Phe Ala Leu Ala Ile Phe Tyr         
145                 150                 155                 160         

ggt gtg tct cag gcc ttc att ggc ctg aac gtc cag cac gac gcc aac      528
Gly Val Ser Gln Ala Phe Ile Gly Leu Asn Val Gln His Asp Ala Asn         
                165                 170                 175             

cac gga gct gcc tcc aaa aag ccc tgg atc aac aat ttg ctc ggc ctg      576
His Gly Ala Ala Ser Lys Lys Pro Trp Ile Asn Asn Leu Leu Gly Leu         
            180                 185                 190                 

ggt gcc gac ttt atc gga ggc tcc aag tgg ctc tgg atg aac cag cac      624
Gly Ala Asp Phe Ile Gly Gly Ser Lys Trp Leu Trp Met Asn Gln His         
        195                 200                 205                     

tgg acc cat cac act tac acc aac cat cac gag aag gat ccc gac gcc      672
Trp Thr His His Thr Tyr Thr Asn His His Glu Lys Asp Pro Asp Ala         
    210                 215                 220                         

ctg ggt gca gag cct atg ctg ctc ttc aac gac tat ccc ttg ggt cac      720
Leu Gly Ala Glu Pro Met Leu Leu Phe Asn Asp Tyr Pro Leu Gly His         
225                 230                 235                 240         

ccc aag cga acc ctc att cat cac ttc caa gcc ttc tac tat ctg ttt      768
Pro Lys Arg Thr Leu Ile His His Phe Gln Ala Phe Tyr Tyr Leu Phe         
                245                 250                 255             

gtc ctt gct ggc tac tgg gtg tct tcg gtg ttc aac cct cag atc ctg      816
Val Leu Ala Gly Tyr Trp Val Ser Ser Val Phe Asn Pro Gln Ile Leu         
            260                 265                 270                 

gac ctc cag cac cga ggt gcc cag gct gtc ggc atg aag atg gag aac      864
Asp Leu Gln His Arg Gly Ala Gln Ala Val Gly Met Lys Met Glu Asn         
        275                 280                 285                     

gac tac att gcc aag tct cga aag tac gct atc ttc ctg cga ctc ctg      912
Asp Tyr Ile Ala Lys Ser Arg Lys Tyr Ala Ile Phe Leu Arg Leu Leu         
    290                 295                 300                         

tac atc tac acc aac att gtg gct ccc atc cag aac caa ggc ttt tcg      960
Tyr Ile Tyr Thr Asn Ile Val Ala Pro Ile Gln Asn Gln Gly Phe Ser         
305                 310                 315                 320         

ctc acc gtc gtt gct cac att ctt act atg ggt gtc gcc tcc agc ctg     1008
Leu Thr Val Val Ala His Ile Leu Thr Met Gly Val Ala Ser Ser Leu         
                325                 330                 335             

acc ctc gct act ctg ttc gcc ctc tcc cac aac ttc gag aac gca gat     1056
Thr Leu Ala Thr Leu Phe Ala Leu Ser His Asn Phe Glu Asn Ala Asp         
            340                 345                 350                 

cgg gat ccc acc tac gag gct cga aag gga ggc gag cct gtc tgt tgg     1104
Arg Asp Pro Thr Tyr Glu Ala Arg Lys Gly Gly Glu Pro Val Cys Trp         
        355                 360                 365                     

ttc aag tcg cag gtg gaa acc tcc tct act tac ggt ggc ttc att tcc     1152
Phe Lys Ser Gln Val Glu Thr Ser Ser Thr Tyr Gly Gly Phe Ile Ser         
    370                 375                 380                         

ggt tgc ctt aca ggc gga ctc aac ttt cag gtc gag cat cac ctg ttt     1200
Gly Cys Leu Thr Gly Gly Leu Asn Phe Gln Val Glu His His Leu Phe         
385                 390                 395                 400         

cct cga atg tcc tct gcc tgg tac ccc tac atc gct cct acc gtt cga     1248
Pro Arg Met Ser Ser Ala Trp Tyr Pro Tyr Ile Ala Pro Thr Val Arg         
                405                 410                 415             

gag gtc tgc aaa aag cac ggc gtc aag tac gcc tac tat ccc tgg gtg     1296
Glu Val Cys Lys Lys His Gly Val Lys Tyr Ala Tyr Tyr Pro Trp Val         
            420                 425                 430                 

tgg cag aac ctc atc tcg acc gtc aag tac ctg cat cag tcc gga act     1344
Trp Gln Asn Leu Ile Ser Thr Val Lys Tyr Leu His Gln Ser Gly Thr         
        435                 440                 445                     

ggc tcg aac tgg aag aac ggt gcc aat ccc tac tct ggc aag ctg taa     1392
Gly Ser Asn Trp Lys Asn Gly Ala Asn Pro Tyr Ser Gly Lys Leu             
    450                 455                 460                         


<210>  46
<211>  463
<212>  PRT
<213>  Peridinium sp. CCMP626

<400>  46

Met Ala Pro Asp Ala Asp Lys Leu Arg Gln Arg Lys Ala Gln Ser Ile 
1               5                   10                  15      


Gln Asp Thr Ala Asp Ser Gln Ala Thr Glu Leu Lys Ile Gly Thr Leu 
            20                  25                  30          


Lys Gly Leu Gln Gly Thr Glu Ile Val Ile Asp Gly Asp Ile Tyr Asp 
        35                  40                  45              


Ile Lys Asp Phe Asp His Pro Gly Gly Glu Ser Ile Met Thr Phe Gly 
    50                  55                  60                  


Gly Asn Asp Val Thr Ala Thr Tyr Lys Met Ile His Pro Tyr His Ser 
65                  70                  75                  80  


Lys His His Leu Glu Lys Met Lys Lys Val Gly Arg Val Pro Asp Tyr 
                85                  90                  95      


Thr Ser Glu Tyr Lys Phe Asp Thr Pro Phe Glu Arg Glu Ile Lys Gln 
            100                 105                 110         


Glu Val Phe Lys Ile Val Arg Arg Gly Arg Glu Phe Gly Thr Pro Gly 
        115                 120                 125             


Tyr Phe Phe Arg Ala Phe Cys Tyr Ile Gly Leu Phe Phe Tyr Leu Gln 
    130                 135                 140                 


Tyr Leu Trp Val Thr Thr Pro Thr Thr Phe Ala Leu Ala Ile Phe Tyr 
145                 150                 155                 160 


Gly Val Ser Gln Ala Phe Ile Gly Leu Asn Val Gln His Asp Ala Asn 
                165                 170                 175     


His Gly Ala Ala Ser Lys Lys Pro Trp Ile Asn Asn Leu Leu Gly Leu 
            180                 185                 190         


Gly Ala Asp Phe Ile Gly Gly Ser Lys Trp Leu Trp Met Asn Gln His 
        195                 200                 205             


Trp Thr His His Thr Tyr Thr Asn His His Glu Lys Asp Pro Asp Ala 
    210                 215                 220                 


Leu Gly Ala Glu Pro Met Leu Leu Phe Asn Asp Tyr Pro Leu Gly His 
225                 230                 235                 240 


Pro Lys Arg Thr Leu Ile His His Phe Gln Ala Phe Tyr Tyr Leu Phe 
                245                 250                 255     


Val Leu Ala Gly Tyr Trp Val Ser Ser Val Phe Asn Pro Gln Ile Leu 
            260                 265                 270         


Asp Leu Gln His Arg Gly Ala Gln Ala Val Gly Met Lys Met Glu Asn 
        275                 280                 285             


Asp Tyr Ile Ala Lys Ser Arg Lys Tyr Ala Ile Phe Leu Arg Leu Leu 
    290                 295                 300                 


Tyr Ile Tyr Thr Asn Ile Val Ala Pro Ile Gln Asn Gln Gly Phe Ser 
305                 310                 315                 320 


Leu Thr Val Val Ala His Ile Leu Thr Met Gly Val Ala Ser Ser Leu 
                325                 330                 335     


Thr Leu Ala Thr Leu Phe Ala Leu Ser His Asn Phe Glu Asn Ala Asp 
            340                 345                 350         


Arg Asp Pro Thr Tyr Glu Ala Arg Lys Gly Gly Glu Pro Val Cys Trp 
        355                 360                 365             


Phe Lys Ser Gln Val Glu Thr Ser Ser Thr Tyr Gly Gly Phe Ile Ser 
    370                 375                 380                 


Gly Cys Leu Thr Gly Gly Leu Asn Phe Gln Val Glu His His Leu Phe 
385                 390                 395                 400 


Pro Arg Met Ser Ser Ala Trp Tyr Pro Tyr Ile Ala Pro Thr Val Arg 
                405                 410                 415     


Glu Val Cys Lys Lys His Gly Val Lys Tyr Ala Tyr Tyr Pro Trp Val 
            420                 425                 430         


Trp Gln Asn Leu Ile Ser Thr Val Lys Tyr Leu His Gln Ser Gly Thr 
        435                 440                 445             


Gly Ser Asn Trp Lys Asn Gly Ala Asn Pro Tyr Ser Gly Lys Leu 
    450                 455                 460             


<210>  47
<211>  1350
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(1350)
<223>  delta-5 desaturase

<400>  47
atg gct ctc agt ctt acc aca gaa cag ctg tta gaa cgc cct gat ttg       48
Met Ala Leu Ser Leu Thr Thr Glu Gln Leu Leu Glu Arg Pro Asp Leu         
1               5                   10                  15              

gtt gcg att gat ggc atc ctc tac gac ctt gaa ggg ctt gcc aaa gtt       96
Val Ala Ile Asp Gly Ile Leu Tyr Asp Leu Glu Gly Leu Ala Lys Val         
            20                  25                  30                  

cat cca gga gga gat ttg att ctc gct tct ggt gcc tct gat gcc tcc      144
His Pro Gly Gly Asp Leu Ile Leu Ala Ser Gly Ala Ser Asp Ala Ser         
        35                  40                  45                      

cct ctc ttt tat tca atg cat cca tac gtc aaa ccg gag aat tcc aaa      192
Pro Leu Phe Tyr Ser Met His Pro Tyr Val Lys Pro Glu Asn Ser Lys         
    50                  55                  60                          

ttg ctt caa cag ttc gtc cga ggg aag cat gac cgc acc tcg aag gac      240
Leu Leu Gln Gln Phe Val Arg Gly Lys His Asp Arg Thr Ser Lys Asp         
65                  70                  75                  80          

att gtc tac acg tat gat tct ccc ttc gca caa gac gtt aag cgg aca      288
Ile Val Tyr Thr Tyr Asp Ser Pro Phe Ala Gln Asp Val Lys Arg Thr         
                85                  90                  95              

atg cgc gag gtg atg aaa ggg agg aac tgg tac gca acc cct ggc ttc      336
Met Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly Phe         
            100                 105                 110                 

tgg ctg cgc acc gtt ggg atc atc gcc gtg acg gcc ttt tgc gag tgg      384
Trp Leu Arg Thr Val Gly Ile Ile Ala Val Thr Ala Phe Cys Glu Trp         
        115                 120                 125                     

cac tgg gct acc acg ggg atg gtg ctg tgg ggc ctg ttg act gga ttc      432
His Trp Ala Thr Thr Gly Met Val Leu Trp Gly Leu Leu Thr Gly Phe         
    130                 135                 140                         

atg cac atg cag atc ggc tta tcc atc cag cat gat gcg tcc cac ggg      480
Met His Met Gln Ile Gly Leu Ser Ile Gln His Asp Ala Ser His Gly         
145                 150                 155                 160         

gcc atc agc aag aag cct tgg gtc aac gcc ctc ttc gcc tac ggc att      528
Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Leu Phe Ala Tyr Gly Ile         
                165                 170                 175             

gac gtc atc gga tcg tcc cgg tgg att tgg ctg cag tcg cac atc atg      576
Asp Val Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile Met         
            180                 185                 190                 

cgg cac cac acc tac acc aac cag cac ggc ctc gac ctg gat gcg gag      624
Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala Glu         
        195                 200                 205                     

tcg gca gag ccg ttc ctg gtg ttc cac aac tac ccc gcc gca aac acc      672
Ser Ala Glu Pro Phe Leu Val Phe His Asn Tyr Pro Ala Ala Asn Thr         
    210                 215                 220                         

gcc cga aag tgg ttc cac cgc ttc caa gct tgg tac atg tac ctt gtg      720
Ala Arg Lys Trp Phe His Arg Phe Gln Ala Trp Tyr Met Tyr Leu Val         
225                 230                 235                 240         

ctg ggg gca tac ggg gta tcg ctg gtg tac aac ccg ctc tac att ttc      768
Leu Gly Ala Tyr Gly Val Ser Leu Val Tyr Asn Pro Leu Tyr Ile Phe         
                245                 250                 255             

cgg atg cag cac aat gac acc atc cca gag tct gtc acg gcc atg cgg      816
Arg Met Gln His Asn Asp Thr Ile Pro Glu Ser Val Thr Ala Met Arg         
            260                 265                 270                 

gag aat ggc ttt ctg cgg cgc tac cgc aca ctt gca ttc gtg atg cga      864
Glu Asn Gly Phe Leu Arg Arg Tyr Arg Thr Leu Ala Phe Val Met Arg         
        275                 280                 285                     

gct ttc ttc atc ttc cgg acc gca ttc ttg ccc tgg tac ctc act ggg      912
Ala Phe Phe Ile Phe Arg Thr Ala Phe Leu Pro Trp Tyr Leu Thr Gly         
    290                 295                 300                         

acc tca ttg ctg atc acc att cct ctg gtg ccc act gca act ggt gcc      960
Thr Ser Leu Leu Ile Thr Ile Pro Leu Val Pro Thr Ala Thr Gly Ala         
305                 310                 315                 320         

ttc ttg acg ttc ttc ttc att ttg tcc cac aat ttt gat ggc tcc gaa     1008
Phe Leu Thr Phe Phe Phe Ile Leu Ser His Asn Phe Asp Gly Ser Glu         
                325                 330                 335             

cgg atc ccc gac aag aac tgc aag gtt aag agc tct gag aag gac gtt     1056
Arg Ile Pro Asp Lys Asn Cys Lys Val Lys Ser Ser Glu Lys Asp Val         
            340                 345                 350                 

gag gct gac caa att gac tgg tat cgg gcg cag gtg gag acg tcc tcc     1104
Glu Ala Asp Gln Ile Asp Trp Tyr Arg Ala Gln Val Glu Thr Ser Ser         
        355                 360                 365                     

aca tac ggt ggc ccc atc gcc atg ttc ttc act ggc ggt ctc aat ttc     1152
Thr Tyr Gly Gly Pro Ile Ala Met Phe Phe Thr Gly Gly Leu Asn Phe         
    370                 375                 380                         

cag atc gag cac cac ctc ttt ccc cgg atg tcg tct tgg cac tac ccc     1200
Gln Ile Glu His His Leu Phe Pro Arg Met Ser Ser Trp His Tyr Pro         
385                 390                 395                 400         

ttc gtc cag cag gcg gtc cgg gag tgt tgc gaa cgc cat gga gtg cga     1248
Phe Val Gln Gln Ala Val Arg Glu Cys Cys Glu Arg His Gly Val Arg         
                405                 410                 415             

tat gtt ttc tac cct acc atc gtc ggc aac atc atc tcc acc ctg aag     1296
Tyr Val Phe Tyr Pro Thr Ile Val Gly Asn Ile Ile Ser Thr Leu Lys         
            420                 425                 430                 

tac atg cat aag gtg ggt gtc gtc cac tgc gtg aag gac gca cag gat     1344
Tyr Met His Lys Val Gly Val Val His Cys Val Lys Asp Ala Gln Asp         
        435                 440                 445                     

tcc tga                                                             1350
Ser                                                                     
                                                                        


<210>  48
<211>  449
<212>  PRT
<213>  Euglena gracilis

<400>  48

Met Ala Leu Ser Leu Thr Thr Glu Gln Leu Leu Glu Arg Pro Asp Leu 
1               5                   10                  15      


Val Ala Ile Asp Gly Ile Leu Tyr Asp Leu Glu Gly Leu Ala Lys Val 
            20                  25                  30          


His Pro Gly Gly Asp Leu Ile Leu Ala Ser Gly Ala Ser Asp Ala Ser 
        35                  40                  45              


Pro Leu Phe Tyr Ser Met His Pro Tyr Val Lys Pro Glu Asn Ser Lys 
    50                  55                  60                  


Leu Leu Gln Gln Phe Val Arg Gly Lys His Asp Arg Thr Ser Lys Asp 
65                  70                  75                  80  


Ile Val Tyr Thr Tyr Asp Ser Pro Phe Ala Gln Asp Val Lys Arg Thr 
                85                  90                  95      


Met Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly Phe 
            100                 105                 110         


Trp Leu Arg Thr Val Gly Ile Ile Ala Val Thr Ala Phe Cys Glu Trp 
        115                 120                 125             


His Trp Ala Thr Thr Gly Met Val Leu Trp Gly Leu Leu Thr Gly Phe 
    130                 135                 140                 


Met His Met Gln Ile Gly Leu Ser Ile Gln His Asp Ala Ser His Gly 
145                 150                 155                 160 


Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Leu Phe Ala Tyr Gly Ile 
                165                 170                 175     


Asp Val Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile Met 
            180                 185                 190         


Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala Glu 
        195                 200                 205             


Ser Ala Glu Pro Phe Leu Val Phe His Asn Tyr Pro Ala Ala Asn Thr 
    210                 215                 220                 


Ala Arg Lys Trp Phe His Arg Phe Gln Ala Trp Tyr Met Tyr Leu Val 
225                 230                 235                 240 


Leu Gly Ala Tyr Gly Val Ser Leu Val Tyr Asn Pro Leu Tyr Ile Phe 
                245                 250                 255     


Arg Met Gln His Asn Asp Thr Ile Pro Glu Ser Val Thr Ala Met Arg 
            260                 265                 270         


Glu Asn Gly Phe Leu Arg Arg Tyr Arg Thr Leu Ala Phe Val Met Arg 
        275                 280                 285             


Ala Phe Phe Ile Phe Arg Thr Ala Phe Leu Pro Trp Tyr Leu Thr Gly 
    290                 295                 300                 


Thr Ser Leu Leu Ile Thr Ile Pro Leu Val Pro Thr Ala Thr Gly Ala 
305                 310                 315                 320 


Phe Leu Thr Phe Phe Phe Ile Leu Ser His Asn Phe Asp Gly Ser Glu 
                325                 330                 335     


Arg Ile Pro Asp Lys Asn Cys Lys Val Lys Ser Ser Glu Lys Asp Val 
            340                 345                 350         


Glu Ala Asp Gln Ile Asp Trp Tyr Arg Ala Gln Val Glu Thr Ser Ser 
        355                 360                 365             


Thr Tyr Gly Gly Pro Ile Ala Met Phe Phe Thr Gly Gly Leu Asn Phe 
    370                 375                 380                 


Gln Ile Glu His His Leu Phe Pro Arg Met Ser Ser Trp His Tyr Pro 
385                 390                 395                 400 


Phe Val Gln Gln Ala Val Arg Glu Cys Cys Glu Arg His Gly Val Arg 
                405                 410                 415     


Tyr Val Phe Tyr Pro Thr Ile Val Gly Asn Ile Ile Ser Thr Leu Lys 
            420                 425                 430         


Tyr Met His Lys Val Gly Val Val His Cys Val Lys Asp Ala Gln Asp 
        435                 440                 445             


Ser 
    


<210>  49
<211>  13066
<212>  DNA
<213>  Artificial Sequence 

<220>
<223>  Plasmid pZP3-Pa777U

<400>  49
tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa     60

atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt    120

cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tcaggtggca    180

cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata    240

tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga    300

gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc    360

ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg    420

cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc    480

ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat    540

cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact    600

tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat    660

tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga    720

tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc    780

ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga    840

tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag    900

cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc    960

gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt   1020

ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct   1080

acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg   1140

cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg   1200

atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca   1260

tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga   1320

tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa   1380

aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga   1440

aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt   1500

taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt   1560

taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat   1620

agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct   1680

tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca   1740

cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag   1800

agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc   1860

gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga   1920

aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca   1980

tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag   2040

ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg   2100

aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct   2160

ggcgcgccac caatcacaat tctgaaaagc acatcttgat ctcctcattg cggggagtcc   2220

aacggtggtc ttattccccc gaatttcccg ctcaatctcg ttccagaccg acccggacac   2280

agtgcttaac gccgttccga aactctaccg cagatatgct ccaacggact gggctgcata   2340

gatgtgatcc tcggcttgga gaaatggata aaagccggcc aaaaaaaaag cggaaaaaag   2400

cggaaaaaaa gagaaaaaaa atcgcaaaat ttgaaaaata gggggaaaag acgcaaaaac   2460

gcaaggaggg gggagtatat gacactgata agcaagctca caacggttcc tcttattttt   2520

ttcctcatct tctgcctagg ttcccaaaat cccagatgct tctctccagt gccaaaagta   2580

agtaccccac aggttttcgg ccgaaaattc cacgtgcagc aacgtcgtgt ggggtgttaa   2640

aatgtggggg gggggaacca ggacaagagg ctcttgtggg agccgaatga gagcacaaag   2700

cgggcgggtg tgataagggc atttttgccc attttccctt ctcctgtctc tccgacggtg   2760

atggcgttgt gcgtcctcta tttcttttta tttctttttg ttttatttct ctgactaccg   2820

atttggtttg atttcctcaa ccccacacaa ataagctcgg gccgaggaat atatatatac   2880

acggacacag tcgccctgtg gacaacacgt cactacctct acgatacaca ccgtacgttg   2940

tgtggaagct tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg   3000

ccaagctcga aattaaccct cactaaaggg aacaaaagct ggagctccac cgcggacaca   3060

atatctggtc aaatttcagt ttcgttacat ttaaattcct tcacttcaag ttcattcttc   3120

atctgcttct gttttacttt gacaggcaaa tgaagacatg gtacgacttg atggaggcca   3180

agaacgccat ttcaccccga gacaccgaag tgcctgaaat cctggctgcc cccattgata   3240

acatcggaaa ctacggtatt ccggaaagtg tatatagaac ctttccccag cttgtgtctg   3300

tggatatgga tggtgtaatc ccctttgagt actcgtcttg gcttctctcc gagcagtatg   3360

aggctctcta atctagcgca tttaatatct caatgtattt atatatttat cttctcatgc   3420

ggccgcttag ttggctttgg tcttggcagc cttggcctcc ttgagggtaa acatcttggc   3480

atccttgtcg accacgccgt acttggcgta cataagacca attcggatga aggtgggaat   3540

gatgggagaa gccgactttc gcaccagttc gggaaaggcc tgagcgaagg cagcagtggc   3600

ctcgttgagc ttgtagtgag gaatgatggg aaacagatgg tggatctgat gtgtaccaat   3660

gttgtgggac aggttgtcga tgagggctcc gtagcttcgg tccacagagg acaagttgcc   3720

cttgacatag gtccactccg aatcggcgta ccagggagtt tcctcgtcgt tgtgatggag   3780

gaaggtagtg acaaccagca tggtggcgaa tccaaagaga ggtgcgaagt aatacagagc   3840

catggtcttg aggccgtaga cgtaggtaag gtaggcgtac agaccagcaa aggccacgag   3900

agagccgagg gaaatgatga cggcagacat tcttcgcagg tagagaggct cccagggatt   3960

gaagtggttg acctttcggg gaggaaatcc agcaacgagg taggcaaacc aagccgaacc   4020

aagggagatg accatgtgtc gggacagggg atgagagtcg gcttctcgct gagggtagaa   4080

gatctcatcc ttgtcgatgt tgccggtgtt cttgtgatgg tgtcgatggc tgatcttcca   4140

cgactcgtag ggagtcagaa tgatggagtg aatgagtgtg ccaacagaga agttgagcag   4200

gtgggatcgc gagaaggcac catgtccaca gtcgtgaccg atggtaaaga atccccagaa   4260

cacgataccc tggagcagaa tgtagccagt gcaaaggacg gcatcgagca gtgcaaactc   4320

ctgcacgata gcaagggctc gagcatagta cagtccgaga gcaagggaac cggcaatgcc   4380

cagagctcgc acggtatagt agagggacca gggaacagag gcttcgaagc agtgggcagg   4440

cagggatcgc ttgatctcgg tgagagtagg gaactcgtag ggagcggcaa cggtagagga   4500

agccatggtt gtgaattagg gtggtgagaa tggttggttg tagggaagaa tcaaaggccg   4560

gtctcgggat ccgtgggtat atatatatat atatatatat acgatccttc gttacctccc   4620

tgttctcaaa actgtggttt ttcgtttttc gttttttgct ttttttgatt tttttagggc   4680

caactaagct tccagatttc gctaatcacc tttgtactaa ttacaagaaa ggaagaagct   4740

gattagagtt gggcttttta tgcaactgtg ctactcctta tctctgatat gaaagtgtag   4800

acccaatcac atcatgtcat ttagagttgg taatactggg aggatagata aggcacgaaa   4860

acgagccata gcagacatgc tgggtgtagc caagcagaag aaagtagatg ggagccaatt   4920

gacgagcgag ggagctacgc caatccgaca tacgacacgc tgagatcgtc ttggccgggg   4980

ggtacctaca gatgtccaag ggtaagtgct tgactgtaat tgtatgtctg aggacaaata   5040

tgtagtcagc cgtataaagt cataccaggc accagtgcca tcatcgaacc actaactctc   5100

tatgatacat gcctccggta ttattgtacc atgcgtcgct ttgttacata cgtatcttgc   5160

ctttttctct cagaaactcc agactttggc tattggtcga gataagcccg gaccatagtg   5220

agtctttcac actctacatt tctcccttgc tccaactatc gattgttgtc tactaactat   5280

cgtacgataa cttcgtatag catacattat acgaagttat cgcgtcgacg agtatctgtc   5340

tgactcgtca ttgccgcctt tggagtacga ctccaactat gagtgtgctt ggatcacttt   5400

gacgatacat tcttcgttgg aggctgtggg tctgacagct gcgttttcgg cgcggttggc   5460

cgacaacaat atcagctgca acgtcattgc tggctttcat catgatcaca tttttgtcgg   5520

caaaggcgac gcccagagag ccattgacgt tctttctaat ttggaccgat agccgtatag   5580

tccagtctat ctataagttc aactaactcg taactattac cataacatat acttcactgc   5640

cccagataag gttccgataa aaagttctgc agactaaatt tatttcagtc tcctcttcac   5700

caccaaaatg ccctcctacg aagctcgagc taacgtccac aagtccgcct ttgccgctcg   5760

agtgctcaag ctcgtggcag ccaagaaaac caacctgtgt gcttctctgg atgttaccac   5820

caccaaggag ctcattgagc ttgccgataa ggtcggacct tatgtgtgca tgatcaaaac   5880

ccatatcgac atcattgacg acttcaccta cgccggcact gtgctccccc tcaaggaact   5940

tgctcttaag cacggtttct tcctgttcga ggacagaaag ttcgcagata ttggcaacac   6000

tgtcaagcac cagtaccggt gtcaccgaat cgccgagtgg tccgatatca ccaacgccca   6060

cggtgtaccc ggaaccggaa tcattgctgg cctgcgagct ggtgccgagg aaactgtctc   6120

tgaacagaag aaggaggacg tctctgacta cgagaactcc cagtacaagg agttcctagt   6180

cccctctccc aacgagaagc tggccagagg tctgctcatg ctggccgagc tgtcttgcaa   6240

gggctctctg gccactggcg agtactccaa gcagaccatt gagcttgccc gatccgaccc   6300

cgagtttgtg gttggcttca ttgcccagaa ccgacctaag ggcgactctg aggactggct   6360

tattctgacc cccggggtgg gtcttgacga caagggagac gctctcggac agcagtaccg   6420

aactgttgag gatgtcatgt ctaccggaac ggatatcata attgtcggcc gaggtctgta   6480

cggccagaac cgagatccta ttgaggaggc caagcgatac cagaaggctg gctgggaggc   6540

ttaccagaag attaactgtt agaggttaga ctatggatat gtaatttaac tgtgtatata   6600

gagagcgtgc aagtatggag cgcttgttca gcttgtatga tggtcagacg acctgtctga   6660

tcgagtatgt atgatactgc acaacctgtg tatccgcatg atctgtccaa tggggcatgt   6720

tgttgtgttt ctcgatacgg agatgctggg tacagtgcta atacgttgaa ctacttatac   6780

ttatatgagg ctcgaagaaa gctgacttgt gtatgactta ttctcaacta catccccagt   6840

cacaatacca ccactgcact accactacac caaaaccatg atcaaaccac ccatggactt   6900

cctggaggca gaagaacttg ttatggaaaa gctcaagaga gagatcataa cttcgtatag   6960

catacattat acgaagttat cctgcaggta aaggaattca tgctgttcat cgtggttaat   7020

gctgctgtgt gctgtgtgtg tgtgttgttt ggcgctcatt gttgcgttat gcagcgtaca   7080

ccacaatatt ggaagcttat tagcctttct attttttcgt ttgcaaggct taacaacatt   7140

gctgtggaga gggatgggga tatggaggcc gctggaggga gtcggagagg cgttttggag   7200

cggcttggcc tggcgcccag ctcgcgaaac gcacctagga ccctttggca cgccgaaatg   7260

tgccactttt cagtctagta acgccttacc tacgtcattc catgcgtgca tgtttgcgcc   7320

ttttttccct tgcccttgat cgccacacag tacagtgcac tgtacagtgg aggttttggg   7380

ggggtcttag atgggagcta aaagcggcct agcggtacac tagtgggatt gtatggagtg   7440

gcatggagcc taggtggagc ctgacaggac gcacgaccgg ctagcccgtg acagacgatg   7500

ggtggctcct gttgtccacc gcgtacaaat gtttgggcca aagtcttgtc agccttgctt   7560

gcgaacctaa ttcccaattt tgtcacttcg cacccccatt gatcgagccc taacccctgc   7620

ccatcaggca atccaattaa gctcgcattg tctgccttgt ttagtttggc tcctgcccgt   7680

ttcggcgtcc acttgcacaa acacaaacaa gcattatata taaggctcgt ctctccctcc   7740

caaccacact cacttttttg cccgtcttcc cttgctaaca caaaagtcaa gaacacaaac   7800

aaccacccca acccccttac acacaagaca tatctacagc aatggccatg gcttcttcca   7860

ctgttgctgc gccgtacgag ttcccgacgc tgacggagat caagcgctcg ctgccagcgc   7920

actgctttga ggcctcggtc ccgtggtcgc tctactacac cgtgcgcgcg ctgggcatcg   7980

ccggctcgct cgcgctcggc ctctactacg cgcgcgcgct cgcgatcgtg caggagtttg   8040

ccctgctgga tgcggtgctc tgcacggggt acattctgct gcagggcatc gtattctggg   8100

ggttcttcac catcggccat gactgcggcc acggcgcgtt ctcgcgttcg cacctgctca   8160

acttcagcgt cggcacgctc attcactcga tcatcctcac gccgtacgag tcatggaaga   8220

tctcgcaccg ccaccaccac aagaacacgg gcaacatcga caaggacgag attttctacc   8280

cgcagcgcga ggccgactcg cacccactgt cccgacacat ggtgatctcg ctcggctcgg   8340

cctggttcgc gtacctcgtt gcgggcttcc ctcctcgcaa ggtgaaccac ttcaaccctt   8400

gggaaccgtt gtacctgcgc cgcatgtctg ccgtcatcat ctcactcggc tcgctcgtgg   8460

cgttcgcggg cttgtatgcg tatctcacct acgtctatgg ccttaagacc atggcgctgt   8520

actacttcgc ccctctcttt gggttcgcca cgatgctcgt ggtcactacc tttttgcacc   8580

acaatgacga ggaaacgcca tggtacgccg actcggagtg gacgtacgtc aagggcaacc   8640

tctcgtccgt ggaccgctcg tacggcgcgc tcatcgacaa cctgagccac aacatcggca   8700

cgcaccagat ccaccacctg tttccgatca tcccgcacta caagctgaac gaggcgacgg   8760

cagcgttcgc gcaggcgttc ccggagctcg tgcgcaagag cgcgtcgccg atcatcccga   8820

cgttcatccg catcgggctc atgtacgcca agtacggcgt cgtggacaag gacgccaaga   8880

tgtttacgct caaggaggcc aaggccgcca agaccaaggc caactaggcg gccgcattga   8940

tgattggaaa cacacacatg ggttatatct aggtgagagt tagttggaca gttatatatt   9000

aaatcagcta tgccaacggt aacttcattc atgtcaacga ggaaccagtg actgcaagta   9060

atatagaatt tgaccacctt gccattctct tgcactcctt tactatatct catttatttc   9120

ttatatacaa atcacttctt cttcccagca tcgagctcgg aaacctcatg agcaataaca   9180

tcgtggatct cgtcaataga gggctttttg gactccttgc tgttggccac cttgtccttg   9240

ctgtttaaac agtgtacgca gatctactat agaggaacat ttaaattgcc ccggagaaga   9300

cggccaggcc gcctagatga caaattcaac aactcacagc tgactttctg ccattgccac   9360

tagggggggg cctttttata tggccaagcc aagctctcca cgtcggttgg gctgcaccca   9420

acaataaatg ggtagggttg caccaacaaa gggatgggat ggggggtaga agatacgagg   9480

ataacggggc tcaatggcac aaataagaac gaatactgcc attaagactc gtgatccagc   9540

gactgacacc attgcatcat ctaagggcct caaaactacc tcggaactgc tgcgctgatc   9600

tggacaccac agaggttccg agcactttag gttgcaccaa atgtcccacc aggtgcaggc   9660

agaaaacgct ggaacagcgt gtacagtttg tcttaacaaa aagtgagggc gctgaggtcg   9720

agcagggtgg tgtgacttgt tatagccttt agagctgcga aagcgcgtat ggatttggct   9780

catcaggcca gattgagggt ctgtggacac atgtcatgtt agtgtacttc aatcgccccc   9840

tggatatagc cccgacaata ggccgtggcc tcattttttt gccttccgca catttccatt   9900

gctcggtacc cacaccttgc ttctcctgca cttgccaacc ttaatactgg tttacattga   9960

ccaacatctt acaagcgggg ggcttgtcta gggtatatat aaacagtggc tctcccaatc  10020

ggttgccagt ctcttttttc ctttctttcc ccacagattc gaaatctaaa ctacacatca  10080

cagaattccg agccgtgagt atccacgaca agatcagtgt cgagacgacg cgttttgtgt  10140

aatgacacaa tccgaaagtc gctagcaaca cacactctct acacaaacta acccagctct  10200

ggtaccatgg cttcttccac tgttgctgcg ccgtacgagt tcccgacgct gacggagatc  10260

aagcgctcgc tgccagcgca ctgctttgag gcctcggtcc cgtggtcgct ctactacacc  10320

gtgcgcgcgc tgggcatcgc cggctcgctc gcgctcggcc tctactacgc gcgcgcgctc  10380

gcgatcgtgc aggagtttgc cctgctggat gcggtgctct gcacggggta cattctgctg  10440

cagggcatcg tattctgggg gttcttcacc atcggccatg actgcggcca cggcgcgttc  10500

tcgcgttcgc acctgctcaa cttcagcgtc ggcacgctca ttcactcgat catcctcacg  10560

ccgtacgagt catggaagat ctcgcaccgc caccaccaca agaacacggg caacatcgac  10620

aaggacgaga ttttctaccc gcagcgcgag gccgactcgc acccactgtc ccgacacatg  10680

gtgatctcgc tcggctcggc ctggttcgcg tacctcgttg cgggcttccc tcctcgcaag  10740

gtgaaccact tcaacccttg ggaaccgttg tacctgcgcc gcatgtctgc cgtcatcatc  10800

tcactcggct cgctcgtggc gttcgcgggc ttgtatgcgt atctcaccta cgtctatggc  10860

cttaagacca tggcgctgta ctacttcgcc cctctctttg ggttcgccac gatgctcgtg  10920

gtcactacct ttttgcacca caatgacgag gaaacgccat ggtacgccga ctcggagtgg  10980

acgtacgtca agggcaacct ctcgtccgtg gaccgctcgt acggcgcgct catcgacaac  11040

ctgagccaca acatcggcac gcaccagatc caccacctgt ttccgatcat cccgcactac  11100

aagctgaacg aggcgacggc agcgttcgcg caggcgttcc cggagctcgt gcgcaagagc  11160

gcgtcgccga tcatcccgac gttcatccgc atcgggctca tgtacgccaa gtacggcgtc  11220

gtggacaagg acgccaagat gtttacgctc aaggaggcca aggccgccaa gaccaaggcc  11280

aactaggcgg ccgcatggag cgtgtgttct gagtcgatgt tttctatgga gttgtgagtg  11340

ttagtagaca tgatgggttt atatatgatg aatgaataga tgtgattttg atttgcacga  11400

tggaattgag aactttgtaa acgtacatgg gaatgtatga atgtgggggt tttgtgactg  11460

gataactgac ggtcagtgga cgccgttgtt caaatatcca agagatgcga gaaactttgg  11520

gtcaagtgaa catgtcctct ctgttcaagt aaaccatcaa ctatgggtag tatatttagt  11580

aaggacaaga gttgagattc tttggagtcc tagaaacgta ttttcgcgtt ccaagatcaa  11640

attagtagag taatacgggc acgggaatcc attcatagtc tcaatcctgc aggtgagtta  11700

attaagatga cgacatttgc gagctggacg aggaatagat ggagcgtgtg ttctgagtcg  11760

atgttttcta tggagttgtg agtgttagta gacatgatgg gtttatatat gatgaatgaa  11820

tagatgtgat tttgatttgc acgatggaat tgagaacttt gtaaacgtac atgggaatgt  11880

atgaatgtgg gggttttgtg actggataac tgacggtcag tggacgccgt tgttcaaata  11940

tccaagagat gcgagaaact ttgggtcaag tgaacatgtc ctctctgttc aagtaaacca  12000

tcaactatgg gtagtatatt tagtaaggac aagagttgag attctttgga gtcctagaaa  12060

cgtattttcg cgttccaaga tcaaattagt agagtaatac gggcacggga atccattcat  12120

agtctcaatt ttcccatagg tgtgctacaa ggtgttgaga tgtggtacag taccaccatg  12180

attcgaggta aagagcccag aagtcattga tgaggtcaag aaatacacag atctacagct  12240

caatacaatg aatatcttct ttcatattct tcaggtgaca ccaagggtgt ctattttccc  12300

cagaaatgcg tgaaaaggcg cgtgtgtagc gtggagtatg ggttcggttg gcgtatcctt  12360

catatatcga cgaaatagta gggcaagaga tgacaaaaag tatctatatg tagacagcgt  12420

agaatatgga tttgattggt ataaattcat ttattgcgtg tctcacaaat actctcgata  12480

agttggggtt aaactggaga tggaacaatg tcgatatctc gacgcatgcg acgtcgggcc  12540

caattcgccc tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga  12600

ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag  12660

ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa  12720

tggcgaatgg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc  12780

agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc  12840

tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg  12900

ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca  12960

cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc  13020

tttaatagtg gactcttgtt ccaaactgga acaacactca acccta                 13066


<210>  50
<211>  1080
<212>  DNA
<213>  Pythium aphanidermatum 


<220>
<221>  CDS
<222>  (1)..(1080)
<223>  synthetic delta-17 desaturase (codon-optimized for Yarrowia 
       lipolytica)

<400>  50
atg gct tcc tct acc gtt gcc gct ccc tac gag ttc cct act ctc acc       48
Met Ala Ser Ser Thr Val Ala Ala Pro Tyr Glu Phe Pro Thr Leu Thr         
1               5                   10                  15              

gag atc aag cga tcc ctg cct gcc cac tgc ttc gaa gcc tct gtt ccc       96
Glu Ile Lys Arg Ser Leu Pro Ala His Cys Phe Glu Ala Ser Val Pro         
            20                  25                  30                  

tgg tcc ctc tac tat acc gtg cga gct ctg ggc att gcc ggt tcc ctt      144
Trp Ser Leu Tyr Tyr Thr Val Arg Ala Leu Gly Ile Ala Gly Ser Leu         
        35                  40                  45                      

gct ctc gga ctg tac tat gct cga gcc ctt gct atc gtg cag gag ttt      192
Ala Leu Gly Leu Tyr Tyr Ala Arg Ala Leu Ala Ile Val Gln Glu Phe         
    50                  55                  60                          

gca ctg ctc gat gcc gtc ctt tgc act ggc tac att ctg ctc cag ggt      240
Ala Leu Leu Asp Ala Val Leu Cys Thr Gly Tyr Ile Leu Leu Gln Gly         
65                  70                  75                  80          

atc gtg ttc tgg gga ttc ttt acc atc ggt cac gac tgt gga cat ggt      288
Ile Val Phe Trp Gly Phe Phe Thr Ile Gly His Asp Cys Gly His Gly         
                85                  90                  95              

gcc ttc tcg cga tcc cac ctg ctc aac ttc tct gtt ggc aca ctc att      336
Ala Phe Ser Arg Ser His Leu Leu Asn Phe Ser Val Gly Thr Leu Ile         
            100                 105                 110                 

cac tcc atc att ctg act ccc tac gag tcg tgg aag atc agc cat cga      384
His Ser Ile Ile Leu Thr Pro Tyr Glu Ser Trp Lys Ile Ser His Arg         
        115                 120                 125                     

cac cat cac aag aac acc ggc aac atc gac aag gat gag atc ttc tac      432
His His His Lys Asn Thr Gly Asn Ile Asp Lys Asp Glu Ile Phe Tyr         
    130                 135                 140                         

cct cag cga gaa gcc gac tct cat ccc ctg tcc cga cac atg gtc atc      480
Pro Gln Arg Glu Ala Asp Ser His Pro Leu Ser Arg His Met Val Ile         
145                 150                 155                 160         

tcc ctt ggt tcg gct tgg ttt gcc tac ctc gtt gct gga ttt cct ccc      528
Ser Leu Gly Ser Ala Trp Phe Ala Tyr Leu Val Ala Gly Phe Pro Pro         
                165                 170                 175             

cga aag gtc aac cac ttc aat ccc tgg gag cct ctc tac ctg cga aga      576
Arg Lys Val Asn His Phe Asn Pro Trp Glu Pro Leu Tyr Leu Arg Arg         
            180                 185                 190                 

atg tct gcc gtc atc att tcc ctc ggc tct ctc gtg gcc ttt gct ggt      624
Met Ser Ala Val Ile Ile Ser Leu Gly Ser Leu Val Ala Phe Ala Gly         
        195                 200                 205                     

ctg tac gcc tac ctt acc tac gtc tac ggc ctc aag acc atg gct ctg      672
Leu Tyr Ala Tyr Leu Thr Tyr Val Tyr Gly Leu Lys Thr Met Ala Leu         
    210                 215                 220                         

tat tac ttc gca cct ctc ttt gga ttc gcc acc atg ctg gtt gtc act      720
Tyr Tyr Phe Ala Pro Leu Phe Gly Phe Ala Thr Met Leu Val Val Thr         
225                 230                 235                 240         

acc ttc ctc cat cac aac gac gag gaa act ccc tgg tac gcc gat tcg      768
Thr Phe Leu His His Asn Asp Glu Glu Thr Pro Trp Tyr Ala Asp Ser         
                245                 250                 255             

gag tgg acc tat gtc aag ggc aac ttg tcc tct gtg gac cga agc tac      816
Glu Trp Thr Tyr Val Lys Gly Asn Leu Ser Ser Val Asp Arg Ser Tyr         
            260                 265                 270                 

gga gcc ctc atc gac aac ctg tcc cac aac att ggt aca cat cag atc      864
Gly Ala Leu Ile Asp Asn Leu Ser His Asn Ile Gly Thr His Gln Ile         
        275                 280                 285                     

cac cat ctg ttt ccc atc att cct cac tac aag ctc aac gag gcc act      912
His His Leu Phe Pro Ile Ile Pro His Tyr Lys Leu Asn Glu Ala Thr         
    290                 295                 300                         

gct gcc ttc gct cag gcc ttt ccc gaa ctg gtg cga aag tcg gct tct      960
Ala Ala Phe Ala Gln Ala Phe Pro Glu Leu Val Arg Lys Ser Ala Ser         
305                 310                 315                 320         

ccc atc att ccc acc ttc atc cga att ggt ctt atg tac gcc aag tac     1008
Pro Ile Ile Pro Thr Phe Ile Arg Ile Gly Leu Met Tyr Ala Lys Tyr         
                325                 330                 335             

ggc gtg gtc gac aag gat gcc aag atg ttt acc ctc aag gag gcc aag     1056
Gly Val Val Asp Lys Asp Ala Lys Met Phe Thr Leu Lys Glu Ala Lys         
            340                 345                 350                 

gct gcc aag acc aaa gcc aac taa                                     1080
Ala Ala Lys Thr Lys Ala Asn                                             
        355                                                             


<210>  51
<211>  359
<212>  PRT
<213>  Pythium aphanidermatum 

<400>  51

Met Ala Ser Ser Thr Val Ala Ala Pro Tyr Glu Phe Pro Thr Leu Thr 
1               5                   10                  15      


Glu Ile Lys Arg Ser Leu Pro Ala His Cys Phe Glu Ala Ser Val Pro 
            20                  25                  30          


Trp Ser Leu Tyr Tyr Thr Val Arg Ala Leu Gly Ile Ala Gly Ser Leu 
        35                  40                  45              


Ala Leu Gly Leu Tyr Tyr Ala Arg Ala Leu Ala Ile Val Gln Glu Phe 
    50                  55                  60                  


Ala Leu Leu Asp Ala Val Leu Cys Thr Gly Tyr Ile Leu Leu Gln Gly 
65                  70                  75                  80  


Ile Val Phe Trp Gly Phe Phe Thr Ile Gly His Asp Cys Gly His Gly 
                85                  90                  95      


Ala Phe Ser Arg Ser His Leu Leu Asn Phe Ser Val Gly Thr Leu Ile 
            100                 105                 110         


His Ser Ile Ile Leu Thr Pro Tyr Glu Ser Trp Lys Ile Ser His Arg 
        115                 120                 125             


His His His Lys Asn Thr Gly Asn Ile Asp Lys Asp Glu Ile Phe Tyr 
    130                 135                 140                 


Pro Gln Arg Glu Ala Asp Ser His Pro Leu Ser Arg His Met Val Ile 
145                 150                 155                 160 


Ser Leu Gly Ser Ala Trp Phe Ala Tyr Leu Val Ala Gly Phe Pro Pro 
                165                 170                 175     


Arg Lys Val Asn His Phe Asn Pro Trp Glu Pro Leu Tyr Leu Arg Arg 
            180                 185                 190         


Met Ser Ala Val Ile Ile Ser Leu Gly Ser Leu Val Ala Phe Ala Gly 
        195                 200                 205             


Leu Tyr Ala Tyr Leu Thr Tyr Val Tyr Gly Leu Lys Thr Met Ala Leu 
    210                 215                 220                 


Tyr Tyr Phe Ala Pro Leu Phe Gly Phe Ala Thr Met Leu Val Val Thr 
225                 230                 235                 240 


Thr Phe Leu His His Asn Asp Glu Glu Thr Pro Trp Tyr Ala Asp Ser 
                245                 250                 255     


Glu Trp Thr Tyr Val Lys Gly Asn Leu Ser Ser Val Asp Arg Ser Tyr 
            260                 265                 270         


Gly Ala Leu Ile Asp Asn Leu Ser His Asn Ile Gly Thr His Gln Ile 
        275                 280                 285             


His His Leu Phe Pro Ile Ile Pro His Tyr Lys Leu Asn Glu Ala Thr 
    290                 295                 300                 


Ala Ala Phe Ala Gln Ala Phe Pro Glu Leu Val Arg Lys Ser Ala Ser 
305                 310                 315                 320 


Pro Ile Ile Pro Thr Phe Ile Arg Ile Gly Leu Met Tyr Ala Lys Tyr 
                325                 330                 335     


Gly Val Val Asp Lys Asp Ala Lys Met Phe Thr Leu Lys Glu Ala Lys 
            340                 345                 350         


Ala Ala Lys Thr Lys Ala Asn 
        355                 


<210>  52
<211>  1080
<212>  DNA
<213>  Pythium aphanidermatum  


<220>
<221>  CDS
<222>  (1)..(1080)
<223>  delta-17 desaturase

<400>  52
atg gct tct tcc act gtt gct gcg ccg tac gag ttc ccg acg ctg acg       48
Met Ala Ser Ser Thr Val Ala Ala Pro Tyr Glu Phe Pro Thr Leu Thr         
1               5                   10                  15              

gag atc aag cgc tcg ctg cca gcg cac tgc ttt gag gcc tcg gtc ccg       96
Glu Ile Lys Arg Ser Leu Pro Ala His Cys Phe Glu Ala Ser Val Pro         
            20                  25                  30                  

tgg tcg ctc tac tac acc gtg cgc gcg ctg ggc atc gcc ggc tcg ctc      144
Trp Ser Leu Tyr Tyr Thr Val Arg Ala Leu Gly Ile Ala Gly Ser Leu         
        35                  40                  45                      

gcg ctc ggc ctc tac tac gcg cgc gcg ctc gcg atc gtg cag gag ttt      192
Ala Leu Gly Leu Tyr Tyr Ala Arg Ala Leu Ala Ile Val Gln Glu Phe         
    50                  55                  60                          

gcc ctg ctg gat gcg gtg ctc tgc acg ggg tac att ctg ctg cag ggc      240
Ala Leu Leu Asp Ala Val Leu Cys Thr Gly Tyr Ile Leu Leu Gln Gly         
65                  70                  75                  80          

atc gta ttc tgg ggg ttc ttc acc atc ggc cat gac tgc ggc cac ggc      288
Ile Val Phe Trp Gly Phe Phe Thr Ile Gly His Asp Cys Gly His Gly         
                85                  90                  95              

gcg ttc tcg cgt tcg cac ctg ctc aac ttc agc gtc ggc acg ctc att      336
Ala Phe Ser Arg Ser His Leu Leu Asn Phe Ser Val Gly Thr Leu Ile         
            100                 105                 110                 

cac tcg atc atc ctc acg ccg tac gag tca tgg aag atc tcg cac cgc      384
His Ser Ile Ile Leu Thr Pro Tyr Glu Ser Trp Lys Ile Ser His Arg         
        115                 120                 125                     

cac cac cac aag aac acg ggc aac atc gac aag gac gag att ttc tac      432
His His His Lys Asn Thr Gly Asn Ile Asp Lys Asp Glu Ile Phe Tyr         
    130                 135                 140                         

ccg cag cgc gag gcc gac tcg cac cca ctg tcc cga cac atg gtg atc      480
Pro Gln Arg Glu Ala Asp Ser His Pro Leu Ser Arg His Met Val Ile         
145                 150                 155                 160         

tcg ctc ggc tcg gcc tgg ttc gcg tac ctc gtt gcg ggc ttc cct cct      528
Ser Leu Gly Ser Ala Trp Phe Ala Tyr Leu Val Ala Gly Phe Pro Pro         
                165                 170                 175             

cgc aag gtg aac cac ttc aac cct tgg gaa ccg ttg tac ctg cgc cgc      576
Arg Lys Val Asn His Phe Asn Pro Trp Glu Pro Leu Tyr Leu Arg Arg         
            180                 185                 190                 

atg tct gcc gtc atc atc tca ctc ggc tcg ctc gtg gcg ttc gcg ggc      624
Met Ser Ala Val Ile Ile Ser Leu Gly Ser Leu Val Ala Phe Ala Gly         
        195                 200                 205                     

ttg tat gcg tat ctc acc tac gtc tat ggc ctt aag acc atg gcg ctg      672
Leu Tyr Ala Tyr Leu Thr Tyr Val Tyr Gly Leu Lys Thr Met Ala Leu         
    210                 215                 220                         

tac tac ttc gcc cct ctc ttt ggg ttc gcc acg atg ctc gtg gtc act      720
Tyr Tyr Phe Ala Pro Leu Phe Gly Phe Ala Thr Met Leu Val Val Thr         
225                 230                 235                 240         

acc ttt ttg cac cac aat gac gag gaa acg cca tgg tac gcc gac tcg      768
Thr Phe Leu His His Asn Asp Glu Glu Thr Pro Trp Tyr Ala Asp Ser         
                245                 250                 255             

gag tgg acg tac gtc aag ggc aac ctc tcg tcc gtg gac cgc tcg tac      816
Glu Trp Thr Tyr Val Lys Gly Asn Leu Ser Ser Val Asp Arg Ser Tyr         
            260                 265                 270                 

ggc gcg ctc atc gac aac ctg agc cac aac atc ggc acg cac cag atc      864
Gly Ala Leu Ile Asp Asn Leu Ser His Asn Ile Gly Thr His Gln Ile         
        275                 280                 285                     

cac cac ctg ttt ccg atc atc ccg cac tac aag ctg aac gag gcg acg      912
His His Leu Phe Pro Ile Ile Pro His Tyr Lys Leu Asn Glu Ala Thr         
    290                 295                 300                         

gca gcg ttc gcg cag gcg ttc ccg gag ctc gtg cgc aag agc gcg tcg      960
Ala Ala Phe Ala Gln Ala Phe Pro Glu Leu Val Arg Lys Ser Ala Ser         
305                 310                 315                 320         

ccg atc atc ccg acg ttc atc cgc atc ggg ctc atg tac gcc aag tac     1008
Pro Ile Ile Pro Thr Phe Ile Arg Ile Gly Leu Met Tyr Ala Lys Tyr         
                325                 330                 335             

ggc gtc gtg gac aag gac gcc aag atg ttt acg ctc aag gag gcc aag     1056
Gly Val Val Asp Lys Asp Ala Lys Met Phe Thr Leu Lys Glu Ala Lys         
            340                 345                 350                 

gcc gcc aag acc aag gcc aac tag                                     1080
Ala Ala Lys Thr Lys Ala Asn                                             
        355                                                             


<210>  53
<211>  359
<212>  PRT
<213>  Pythium aphanidermatum  

<400>  53

Met Ala Ser Ser Thr Val Ala Ala Pro Tyr Glu Phe Pro Thr Leu Thr 
1               5                   10                  15      


Glu Ile Lys Arg Ser Leu Pro Ala His Cys Phe Glu Ala Ser Val Pro 
            20                  25                  30          


Trp Ser Leu Tyr Tyr Thr Val Arg Ala Leu Gly Ile Ala Gly Ser Leu 
        35                  40                  45              


Ala Leu Gly Leu Tyr Tyr Ala Arg Ala Leu Ala Ile Val Gln Glu Phe 
    50                  55                  60                  


Ala Leu Leu Asp Ala Val Leu Cys Thr Gly Tyr Ile Leu Leu Gln Gly 
65                  70                  75                  80  


Ile Val Phe Trp Gly Phe Phe Thr Ile Gly His Asp Cys Gly His Gly 
                85                  90                  95      


Ala Phe Ser Arg Ser His Leu Leu Asn Phe Ser Val Gly Thr Leu Ile 
            100                 105                 110         


His Ser Ile Ile Leu Thr Pro Tyr Glu Ser Trp Lys Ile Ser His Arg 
        115                 120                 125             


His His His Lys Asn Thr Gly Asn Ile Asp Lys Asp Glu Ile Phe Tyr 
    130                 135                 140                 


Pro Gln Arg Glu Ala Asp Ser His Pro Leu Ser Arg His Met Val Ile 
145                 150                 155                 160 


Ser Leu Gly Ser Ala Trp Phe Ala Tyr Leu Val Ala Gly Phe Pro Pro 
                165                 170                 175     


Arg Lys Val Asn His Phe Asn Pro Trp Glu Pro Leu Tyr Leu Arg Arg 
            180                 185                 190         


Met Ser Ala Val Ile Ile Ser Leu Gly Ser Leu Val Ala Phe Ala Gly 
        195                 200                 205             


Leu Tyr Ala Tyr Leu Thr Tyr Val Tyr Gly Leu Lys Thr Met Ala Leu 
    210                 215                 220                 


Tyr Tyr Phe Ala Pro Leu Phe Gly Phe Ala Thr Met Leu Val Val Thr 
225                 230                 235                 240 


Thr Phe Leu His His Asn Asp Glu Glu Thr Pro Trp Tyr Ala Asp Ser 
                245                 250                 255     


Glu Trp Thr Tyr Val Lys Gly Asn Leu Ser Ser Val Asp Arg Ser Tyr 
            260                 265                 270         


Gly Ala Leu Ile Asp Asn Leu Ser His Asn Ile Gly Thr His Gln Ile 
        275                 280                 285             


His His Leu Phe Pro Ile Ile Pro His Tyr Lys Leu Asn Glu Ala Thr 
    290                 295                 300                 


Ala Ala Phe Ala Gln Ala Phe Pro Glu Leu Val Arg Lys Ser Ala Ser 
305                 310                 315                 320 


Pro Ile Ile Pro Thr Phe Ile Arg Ile Gly Leu Met Tyr Ala Lys Tyr 
                325                 330                 335     


Gly Val Val Asp Lys Asp Ala Lys Met Phe Thr Leu Lys Glu Ala Lys 
            340                 345                 350         


Ala Ala Lys Thr Lys Ala Asn 
        355                 


<210>  54
<211>  9570
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pY117

<400>  54
ggccgccacc gcggcccgag attccggcct cttcggccgc caagcgaccc gggtggacgt     60

ctagaggtac ctagcaatta acagatagtt tgccggtgat aattctctta acctcccaca    120

ctcctttgac ataacgattt atgtaacgaa actgaaattt gaccagatat tgtgtccgcg    180

gtggagctcc agcttttgtt ccctttagtg agggtttaaa cgagcttggc gtaatcatgg    240

tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa cgtacgagcc    300

ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg    360

ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc    420

ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact    480

gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta    540

atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag    600

caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc    660

cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta    720

taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg    780

ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc    840

tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac    900

gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac    960

ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg   1020

aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga   1080

aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt   1140

agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag   1200

cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct   1260

gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg   1320

atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat   1380

gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc   1440

tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg   1500

gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct   1560

ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca   1620

actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg   1680

ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg   1740

tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc   1800

cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag   1860

ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg   1920

ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag   1980

tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat   2040

agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg   2100

atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca   2160

gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca   2220

aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat   2280

tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag   2340

aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc   2400

tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt   2460

gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc   2520

ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta   2580

cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc   2640

tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg   2700

ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt   2760

ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat   2820

tttaacaaaa tattaacgct tacaatttcc attcgccatt caggctgcgc aactgttggg   2880

aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg   2940

caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg   3000

ccagtgaatt gtaatacgac tcactatagg gcgaattggg taccgggccc cccctcgagg   3060

tcgatggtgt cgataagctt gatatcgaat tcatgtcaca caaaccgatc ttcgcctcaa   3120

ggaaacctaa ttctacatcc gagagactgc cgagatccag tctacactga ttaattttcg   3180

ggccaataat ttaaaaaaat cgtgttatat aatattatat gtattatata tatacatcat   3240

gatgatactg acagtcatgt cccattgcta aatagacaga ctccatctgc cgcctccaac   3300

tgatgttctc aatatttaag gggtcatctc gcattgttta ataataaaca gactccatct   3360

accgcctcca aatgatgttc tcaaaatata ttgtatgaac ttatttttat tacttagtat   3420

tattagacaa cttacttgct ttatgaaaaa cacttcctat ttaggaaaca atttataatg   3480

gcagttcgtt catttaacaa tttatgtaga ataaatgtta taaatgcgta tgggaaatct   3540

taaatatgga tagcataaat gatatctgca ttgcctaatt cgaaatcaac agcaacgaaa   3600

aaaatccctt gtacaacata aatagtcatc gagaaatatc aactatcaaa gaacagctat   3660

tcacacgtta ctattgagat tattattgga cgagaatcac acactcaact gtctttctct   3720

cttctagaaa tacaggtaca agtatgtact attctcattg ttcatacttc tagtcatttc   3780

atcccacata ttccttggat ttctctccaa tgaatgacat tctatcttgc aaattcaaca   3840

attataataa gatataccaa agtagcggta tagtggcaat caaaaagctt ctctggtgtg   3900

cttctcgtat ttatttttat tctaatgatc cattaaaggt atatatttat ttcttgttat   3960

ataatccttt tgtttattac atgggctgga tacataaagg tattttgatt taattttttg   4020

cttaaattca atcccccctc gttcagtgtc aactgtaatg gtaggaaatt accatacttt   4080

tgaagaagca aaaaaaatga aagaaaaaaa aaatcgtatt tccaggttag acgttccgca   4140

gaatctagaa tgcggtatgc ggtacattgt tcttcgaacg taaaagttgc gctccctgag   4200

atattgtaca tttttgcttt tacaagtaca agtacatcgt acaactatgt actactgttg   4260

atgcatccac aacagtttgt tttgtttttt tttgtttttt ttttttctaa tgattcatta   4320

ccgctatgta tacctacttg tacttgtagt aagccgggtt attggcgttc aattaatcat   4380

agacttatga atctgcacgg tgtgcgctgc gagttacttt tagcttatgc atgctacttg   4440

ggtgtaatat tgggatctgt tcggaaatca acggatgctc aaccgatttc gacagtaatt   4500

aattaattcc ctagtcccag tgtacacccg ccgatatcgc ttaccctgca gccggattaa   4560

ggttggcaat ttttcacgtc cttgtctccg caattactca ccgggtggtt tataagattg   4620

caagcgtctt gatttgtctc tgtatactaa catgcaatcg cgactcgccc gacgggccac   4680

taacctggcc agaatctcca gatccaagta ttctcttggt ctgcgatatg tttccaacac   4740

aaaagcccct gctgcccagc cggcaactgc tgagtgagta ttccttgcca taaacgaccc   4800

agaaccactg tatagtgttt ggaagcacta gtcagaagac cagcgaaaac aggtggaaaa   4860

aactgagacg aaaagcaacg accagaaatg taatgtgtgg aaaagcgaca cacacagagc   4920

agataaagag gtgacaaata acgacaaatg aaatatcagt atcttcccac aatcactacc   4980

tctcagctgt ctgaaggtgc ggctgatata tccatcccac gtctaacgta tggagtgtga   5040

tagaatatga cgacacaagc atgagaactc gctctctatc caaccaccga aacactgtca   5100

ctacagccgt tcttgttgct ccattcgctt ttgtgattcc atgccttctc tggtgactga   5160

caacattcct tccttttctc cagccctgtt gttatctgct catgacctac ggccactctc   5220

tatcgcatac taacatagac gatcccagcc cgctccccac ttccagggca ccgttggcaa   5280

gcctcctatc ctcaagaagg ctgaggctgc caacgctgac atggacgagt ccttcatcgg   5340

aatgtctgga ggagagatct tccacgagat gatgctgcga cacaacgtcg acactgtctt   5400

cggttacccc ggtggagcca ttctccccgt ctttgacgcc attcacaact ctgagtactt   5460

caactttgtg ctccctcgac acgagcaggg tgccggccac atggccgagg gctacgctcg   5520

agcctctggt aagcccggtg tcgttctcgt cacctctggc cccggtgcca ccaacgtcat   5580

cacccccatg caggacgctc tttccgatgg tacccccatg gttgtcttca ccggtcaggt   5640

cctgacctcc gttatcggca ctgacgcctt ccaggaggcc gatgttgtcg gcatctcccg   5700

atcttgcacc aagtggaacg tcatggtcaa gaacgttgct gagctccccc gacgaatcaa   5760

cgaggccttt gagattgcta cttccggccg acccggtccc gttctcgtcg atctgcccaa   5820

ggatgttact gctgccatcc tgcgagagcc catccccacc aagtccacca ttccctcgca   5880

ttctctgacc aacctcacct ctgccgccgc caccgagttc cagaagcagg ctatccagcg   5940

agccgccaac ctcatcaacc agtccaagaa gcccgtcctt tacgtcggac agggtatcct   6000

tggctccgag gagggtccta agctgcttaa ggagctggct gagaaggccg agattcccgt   6060

caccactact ctgcagggtc ttggtgcctt tgacgagcga gaccccaagt ctctgcacat   6120

gctcggtatg cacggttccg gctacgccaa catggccatg cagaacgctg actgtatcat   6180

tgctctcggc gcccgatttg atgaccgagt taccggctcc atccccaagt ttgcccccga   6240

ggctcgagcc gctgcccttg agggtcgagg tggtattgtt cactttgaga tccaggccaa   6300

gaacatcaac aaggttgttc aggccaccga agccgttgag ggagacgtta ccgagtctgt   6360

ccgacagctc atccccctca tcaacaaggt ctctgccgct gagcgagctc cctggactga   6420

gactatccag tcctggaagc agcagttccc cttcctcttc gaggctgaag gtgaggatgg   6480

tgttatcaag ccccagtccg tcattgctct gctctctgac ctgacagaga acaacaagga   6540

caagaccatc atcaccaccg gtgttggtca gcatcagatg tggactgccc agcatttccg   6600

atggcgacac cctcgaacca tgatcacttc tggtggtctt ggaactatgg gttacggcct   6660

gcccgccgct atcggcgcca aggttgcccg acctgactgc gacgtcattg acatcgatgg   6720

tgacgcttct ttcaacatga ctctgaccga gctgtccacc gccgttcagt tcaacattgg   6780

cgtcaaggct attgtcctca acaacgagga acagggtatg gtcacccagc tgcagtctct   6840

cttctacgag aaccgatact gccacactca tcagaagaac cccgacttca tgaagctggc   6900

cgagtccatg ggcatgaagg gtatccgaat cactcacatt gaccagctgg aggccggtct   6960

caaggagatg ctcgcataca agggccctgt gctcgttgag gttgttgtcg acaagaagat   7020

ccccgttctt cccatggttc ccgctggtaa ggctttgcat gagttccttg tctacgacgc   7080

tgacgccgag gctgcttctc gacccgatcg actgaagaat gcccccgccc ctcacgtcca   7140

ccagaccacc tttgagaact aagtggaaag gaacacaagc aatccgaacc aaaaataatt   7200

ggggtcccgt gcccacagag tctagtgcag acctaaaatg accacagtaa attatagctg   7260

ttattaaaca tgagattttg accaacaaga gcgtaggaat gttattagct actacttgta   7320

catacacagc atttgtttta aataatgttg cctccagggg cagtgagatc aggacccaga   7380

tccgtggcca gctctctgac ttcagaccgc ttgtacttaa gcagctcgca acactgttgt   7440

cgaggattga acttgccata ttcgattttg tggtcatgaa tccagcacac ctcatttaaa   7500

tgtagctaac ggtagcaggc gaactactgg tacatacctc ccccggaata tgtacaggca   7560

taatgcgtat ctgtgggaca tgtggtcgtt gcgccattat gtaagcagcg tgtactcctc   7620

tgactgtcca tatggtttgc tccatctcac cctcatcgtt ttcattgttc acaggcggcc   7680

acaaaaaaac tgtcttctct ccttctctct tcgccttagt ctactcggac cagttttagt   7740

ttagcttggc gccactggat aaatgagacc tcaggccttg tgatgaggag gtcacttatg   7800

aagcatgtta ggaggtgctt gtatggatag agaagcaccc aaaataataa gaataataat   7860

aaaacagggg gcgttgtcat ttcatatcgt gttttcacca tcaatacacc tccaaacaat   7920

gcccttcatg tggccagccc caatattgtc ctgtagttca actctatgca gctcgtatct   7980

tattgagcaa gtaaaactct gtcagccgat attgcccgac ccgcgacaag ggtcaacaag   8040

gtggtgtaag gccttcgcag aagtcaaaac tgtgccaaac aaacatctag agtctctttg   8100

gtgtttctcg catatatttw atcggctgtc ttacgtattt gcgcctcggt accggactaa   8160

tttcggatca tccccaatac gctttttctt cgcagctgtc aacagtgtcc atgatctatc   8220

cacctaaatg ggtcatatga ggcgtataat ttcgtggtgc tgataataat tcccatatat   8280

ttgacacaaa acttcccccc ctagacatac atctcacaat ctcacttctt gtgcttctgt   8340

cacacatctc ctccagctga cttcaactca cacctctgcc ccagttggtc tacagcggta   8400

taaggtttct ccgcatagag gtgcaccact cctcccgata cttgtttgtg tgacttgtgg   8460

gtcacgacat atatatctac acacattgcg ccaccctttg gttcttccag cacaacaaaa   8520

acacgacacg ctaaccatgg ccaatttact gaccgtacac caaaatttgc ctgcattacc   8580

ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg gacatgttca gggatcgcca   8640

ggcgttttct gagcatacct ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg   8700

gtgcaagttg aataaccgga aatggtttcc cgcagaacct gaagatgttc gcgattatct   8760

tctatatctt caggcgcgcg gtctggcagt aaaaactatc cagcaacatt tgggccagct   8820

aaacatgctt catcgtcggt ccgggctgcc acgaccaagt gacagcaatg ctgtttcact   8880

ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct   8940

agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc atggaaaata gcgatcgctg   9000

ccaggatata cgtaatctgg catttctggg gattgcttat aacaccctgt tacgtatagc   9060

cgaaattgcc aggatcaggg ttaaagatat ctcacgtact gacggtggga gaatgttaat   9120

ccatattggc agaacgaaaa cgctggttag caccgcaggt gtagagaagg cacttagcct   9180

gggggtaact aaactggtcg agcgatggat ttccgtctct ggtgtagctg atgatccgaa   9240

taactacctg ttttgccggg tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca   9300

gctatcaact cgcgccctgg aagggatttt tgaagcaact catcgattga tttacggcgc   9360

taaggatgac tctggtcaga gatacctggc ctggtctgga cacagtgccc gtgtcggagc   9420

cgcgcgagat atggcccgcg ctggagtttc aataccggag atcatgcaag ctggtggctg   9480

gaccaatgta aatattgtca tgaactatat ccgtaacctg gatagtgaaa caggggcaat   9540

ggtgcgcctg ctggaagatg gcgattaagc                                    9570


<210>  55
<211>  15743
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZP2-2988

<400>  55
ggccgcatgt acatacaaga ttatttatag aaatgaatcg cgatcgaaca aagagtacga     60

gtgtacgagt aggggatgat gataaaagtg gaagaagttc cgcatctttg gatttatcaa    120

cgtgtaggac gatacttcct gtaaaaatgc aatgtcttta ccataggttc tgctgtagat    180

gttattaact accattaaca tgtctacttg tacagttgca gaccagttgg agtatagaat    240

ggtacactta ccaaaaagtg ttgatggttg taactacgat atataaaact gttgacggga    300

tctgtatatt cggtaagata tattttgtgg ggttttagtg gtgtttaaac agtgtacgca    360

gtactataga ggaacaattg ccccggagaa gacggccagg ccgcctagat gacaaattca    420

acaactcaca gctgactttc tgccattgcc actagggggg ggccttttta tatggccaag    480

ccaagctctc cacgtcggtt gggctgcacc caacaataaa tgggtagggt tgcaccaaca    540

aagggatggg atggggggta gaagatacga ggataacggg gctcaatggc acaaataaga    600

acgaatactg ccattaagac tcgtgatcca gcgactgaca ccattgcatc atctaagggc    660

ctcaaaacta cctcggaact gctgcgctga tctggacacc acagaggttc cgagcacttt    720

aggttgcacc aaatgtccca ccaggtgcag gcagaaaacg ctggaacagc gtgtacagtt    780

tgtcttaaca aaaagtgagg gcgctgaggt cgagcagggt ggtgtgactt gttatagcct    840

ttagagctgc gaaagcgcgt atggatttgg ctcatcaggc cagattgagg gtctgtggac    900

acatgtcatg ttagtgtact tcaatcgccc cctggatata gccccgacaa taggccgtgg    960

cctcattttt ttgccttccg cacatttcca ttgctcggta cccacacctt gcttctcctg   1020

cacttgccaa ccttaatact ggtttacatt gaccaacatc ttacaagcgg ggggcttgtc   1080

tagggtatat ataaacagtg gctctcccaa tcggttgcca gtctcttttt tcctttcttt   1140

ccccacagat tcgaaatcta aactacacat cacaccatgg aggtcgtgaa cgaaatcgtc   1200

tccattggcc aggaggttct tcccaaggtc gactatgctc agctctggtc tgatgcctcg   1260

cactgcgagg tgctgtacct ctccatcgcc ttcgtcatcc tgaagttcac ccttggtcct   1320

ctcggaccca agggtcagtc tcgaatgaag tttgtgttca ccaactacaa cctgctcatg   1380

tccatctact cgctgggctc cttcctctct atggcctacg ccatgtacac cattggtgtc   1440

atgtccgaca actgcgagaa ggctttcgac aacaatgtct tccgaatcac cactcagctg   1500

ttctacctca gcaagttcct cgagtacatt gactccttct atctgcccct catgggcaag   1560

cctctgacct ggttgcagtt ctttcaccat ctcggagctc ctatggacat gtggctgttc   1620

tacaactacc gaaacgaagc cgtttggatc tttgtgctgc tcaacggctt cattcactgg   1680

atcatgtacg gctactattg gacccgactg atcaagctca agttccctat gcccaagtcc   1740

ctgattactt ctatgcagat cattcagttc aacgttggct tctacatcgt ctggaagtac   1800

cggaacattc cctgctaccg acaagatgga atgagaatgt ttggctggtt tttcaactac   1860

ttctacgttg gtactgtcct gtgtctgttc ctcaacttct acgtgcagac ctacatcgtc   1920

cgaaagcaca agggagccaa aaagattcag tgagcggccg caagtgtgga tggggaagtg   1980

agtgcccggt tctgtgtgca caattggcaa tccaagatgg atggattcaa cacagggata   2040

tagcgagcta cgtggtggtg cgaggatata gcaacggata tttatgtttg acacttgaga   2100

atgtacgata caagcactgt ccaagtacaa tactaaacat actgtacata ctcatactcg   2160

tacccgggca acggtttcac ttgagtgcag tggctagtgc tcttactcgt acagtgtgca   2220

atactgcgta tcatagtctt tgatgtatat cgtattcatt catgttagtt gcgtacgggc   2280

gtcgttgctt gtgtgatttt tgaggaccca tccctttggt atataagtat actctggggt   2340

taaggttgcc cgtgtagtct aggttatagt tttcatgtga aataccgaga gccgagggag   2400

aataaacggg ggtatttgga cttgtttttt tcgcggaaaa gcgtcgaatc aaccctgcgg   2460

gccttgcacc atgtccacga cgtgtttctc gccccaattc gccccttgca cgtcaaaatt   2520

aggcctccat ctagacccct ccataacatg tgactgtggg gaaaagtata agggaaacca   2580

tgcaaccata gacgacgtga aagacgggga ggaaccaatg gaggccaaag aaatggggta   2640

gcaacagtcc aggagacaga caaggagaca aggagagggc gcccgaaaga tcggaaaaac   2700

aaacatgtcc aattggggca gtgacggaaa cgacacggac acttcagtac aatggaccga   2760

ccatctccaa gccagggtta ttccggtatc accttggccg taacctcccg ctggtacctg   2820

atattgtaca cgttcacatt caatatactt tcagctacaa taagagaggc tgtttgtcgg   2880

gcatgtgtgt ccgtcgtatg gggtgatgtc cgagggcgaa attcgctaca agcttaactc   2940

tggcgcttgt ccagtatgaa tagacaagtc aagaccagtg gtgccatgat tgacagggag   3000

gtacaagact tcgatactcg agcattactc ggacttgtgg cgattgaaca gacgggcgat   3060

cgcttctccc ccgtattgcc ggcgcgccag ctgcattaat gaatcggcca acgcgcgggg   3120

agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg   3180

gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca   3240

gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac   3300

cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac   3360

aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg   3420

tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac   3480

ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat   3540

ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag   3600

cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac   3660

ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt   3720

gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt   3780

atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc   3840

aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga   3900

aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac   3960

gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc   4020

cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct   4080

gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca   4140

tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct   4200

ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca   4260

ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc   4320

atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg   4380

cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct   4440

tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa   4500

aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta   4560

tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc   4620

ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg   4680

agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa   4740

gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg   4800

agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc   4860

accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg   4920

gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat   4980

cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata   5040

ggggttccgc gcacatttcc ccgaaaagtg ccacctgatg cggtgtgaaa taccgcacag   5100

atgcgtaagg agaaaatacc gcatcaggaa attgtaagcg ttaatatttt gttaaaattc   5160

gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc   5220

ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag   5280

agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc   5340

gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa   5400

gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg   5460

aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt   5520

gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc   5580

gcgtccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt   5640

cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc   5700

cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac   5760

tatagggcga attgggcccg acgtcgcatg cgctgatgac actttggtct gaaagagatg   5820

cattttgaat cccaaacttg cagtgcccaa gtgacataca tctccgcgtt ttggaaaatg   5880

ttcagaaaca gttgattgtg ttggaatggg gaatggggaa tggaaaaatg actcaagtat   5940

caattccaaa aacttctctg gctggcagta cctactgtcc atactactgc attttctcca   6000

gtcaggccac tctatactcg acgacacagt agtaaaaccc agataatttc gacataaaca   6060

agaaaacaga cccaataata tttatatata gtcagccgtt tgtccagttc agactgtaat   6120

agccgaaaaa aaatccaaag tttctattct aggaaaatat attccaatat ttttaattct   6180

taatctcatt tattttattc tagcgaaata catttcagct acttgagaca tgtgataccc   6240

acaaatcgga ttcggactcg gttgttcaga agagcatatg gcattcgtgc tcgcttgttc   6300

acgtattctt cctgttccat ctcttggccg acaatcacac aaaaatgggg tttttttttt   6360

aattctaatg attcattaca gcaaaattga gatatagcag accacgtatt ccataatcac   6420

caaggaagtt cttgggcgtc ttaattaact cacctgcagg attgagacta tgaatggatt   6480

cccgtgcccg tattactcta ctaatttgat cttggaacgc gaaaatacgt ttctaggact   6540

ccaaagaatc tcaactcttg tccttactaa atatactacc catagttgat ggtttacttg   6600

aacagagagg acatgttcac ttgacccaaa gtttctcgca tctcttggat atttgaacaa   6660

cggcgtccac tgaccgtcag ttatccagtc acaaaacccc cacattcata cattcccatg   6720

tacgtttaca aagttctcaa ttccatcgtg caaatcaaaa tcacatctat tcattcatca   6780

tatataaacc catcatgtct actaacactc acaactccat agaaaacatc gactcagaac   6840

acacgctcca tgcggccgct tactgagcct tggcaccggg ctgcttctcg gccattcgag   6900

cgaactggga caggtatcgg agcaggatga cgagaccttc atggggcaga gggtttcggt   6960

aggggaggtt gtgcttctgg cacagctgtt ccacctggta ggaaacggca gtgaggttgt   7020

gtcgaggcag ggtgggccag agatggtgct cgatctggta gttcaggcct ccaaagaacc   7080

agtcagtaat gatgcctcgt cgaatgttca tggtctcatg gatctgaccc acagagaagc   7140

catgtccgtc ccagacggaa tcaccgatct tctccagagg gtagtggttc atgaagacca   7200

cgatggcaat tccgaagcca ccgacgagct cggaaacaaa gaacaccagc atcgaggtca   7260

ggatggaggg cataaagaag aggtggaaca gggtcttgag agtccagtgc agagcgagtc   7320

caatggcctc tttcttgtac tgagatcggt agaactggtt gtctcggtcc ttgagggatc   7380

gaacggtcag cacagactgg aaacaccaga tgaatcgcag gagaatacag atgaccagga   7440

aatagtactg ttggaactga atgagctttc gggagatggg agaagctcga gtgacatcgt   7500

cctcggacca ggcgagcaga ggcaggttat caatgtcggg atcgtgaccc tgaacgttgg   7560

tagcagaatg atgggcgttg tgtctgtcct tccaccaggt cacggagaag ccctggagtc   7620

cgttgccaaa gaccagaccc aggacgttat tccagtttcg gttcttgaag gtctggtggt   7680

ggcagatgtc atgagacagc catcccattt gctggtagtg cataccgagc acgagagcac   7740

caatgaagta caggtggtac tggaccagca tgaagaaggc aagcacgcca agacccaggg   7800

tggtcaagat cttgtacgag taccagaggg gagaggcgtc aaacatgcca gtggcgatca   7860

gctcttctcg gagctttcgg aaatcctcct gagcttcgtt gacggcagcc tggggaggca   7920

gctcggaagc ctggttgatc ttgggcattc gcttgagctt gtcgaaggct tcctgagagt   7980

gcataaccat gaaggcgtca gtagcatctc gtccctggta gttctcaatg atttcagctc   8040

caccagggtg gaagttcacc caagcggaga cgtcgtacac ctttccgtcg atgacgaggg   8100

gcagagcctg tcgagaagcc ttcaccatgg ttgtgaatta gggtggtgag aatggttggt   8160

tgtagggaag aatcaaaggc cggtctcggg atccgtgggt atatatatat atatatatat   8220

atacgatcct tcgttacctc cctgttctca aaactgtggt ttttcgtttt tcgttttttg   8280

ctttttttga tttttttagg gccaactaag cttccagatt tcgctaatca cctttgtact   8340

aattacaaga aaggaagaag ctgattagag ttgggctttt tatgcaactg tgctactcct   8400

tatctctgat atgaaagtgt agacccaatc acatcatgtc atttagagtt ggtaatactg   8460

ggaggataga taaggcacga aaacgagcca tagcagacat gctgggtgta gccaagcaga   8520

agaaagtaga tgggagccaa ttgacgagcg agggagctac gccaatccga catacgacac   8580

gctgagatcg tcttggccgg ggggtaccta cagatgtcca agggtaagtg cttgactgta   8640

attgtatgtc tgaggacaaa tatgtagtca gccgtataaa gtcataccag gcaccagtgc   8700

catcatcgaa ccactaactc tctatgatac atgcctccgg tattattgta ccatgcgtcg   8760

ctttgttaca tacgtatctt gcctttttct ctcagaaact ccagactttg gctattggtc   8820

gagataagcc cggaccatag tgagtctttc acactctaca tttctccctt gctccaacta   8880

tttaaattcc ttcacttcaa gttcattctt catctgcttc tgttttactt tgacaggcaa   8940

atgaagacat ggtacgactt gatggaggcc aagaacgcca tttcaccccg agacaccgaa   9000

gtgcctgaaa tcctggctgc ccccattgat aacatcggaa actacggtat tccggaaagt   9060

gtatatagaa cctttcccca gcttgtgtct gtggatatgg atggtgtaat cccctttgag   9120

tactcgtctt ggcttctctc cgagcagtat gaggctctct aatctagcgc atttaatatc   9180

tcaatgtatt tatatattta tcttctcatg cggccgctta ctgagccttg gcaccgggct   9240

gcttctcggc cattcgagcg aactgggaca ggtatcggag caggatgacg agaccttcat   9300

ggggcagagg gtttcggtag gggaggttgt gcttctggca cagctgttcc acctggtagg   9360

aaacggcagt gaggttgtgt cgaggcaggg tgggccagag atggtgctcg atctggtagt   9420

tcaggcctcc aaagaaccag tcagtaatga tgcctcgtcg aatgttcatg gtctcatgga   9480

tctgacccac agagaagcca tgtccgtccc agacggaatc accgatcttc tccagagggt   9540

agtggttcat gaagaccacg atggcaattc cgaagccacc gacgagctcg gaaacaaaga   9600

acaccagcat cgaggtcagg atggagggca taaagaagag gtggaacagg gtcttgagag   9660

tccagtgcag agcgagtcca atggcctctt tcttgtactg agatcggtag aactggttgt   9720

ctcggtcctt gagggatcga acggtcagca cagactggaa acaccagatg aatcgcagga   9780

gaatacagat gaccaggaaa tagtactgtt ggaactgaat gagctttcgg gagatgggag   9840

aagctcgagt gacatcgtcc tcggaccagg cgagcagagg caggttatca atgtcgggat   9900

cgtgaccctg aacgttggta gcagaatgat gggcgttgtg tctgtccttc caccaggtca   9960

cggagaagcc ctggagtccg ttgccaaaga ccagacccag gacgttattc cagtttcggt  10020

tcttgaaggt ctggtggtgg cagatgtcat gagacagcca tcccatttgc tggtagtgca  10080

taccgagcac gagagcacca atgaagtaca ggtggtactg gaccagcatg aagaaggcaa  10140

gcacgccaag acccagggtg gtcaagatct tgtacgagta ccagagggga gaggcgtcaa  10200

acatgccagt ggcgatcagc tcttctcgga gctttcggaa atcctcctga gcttcgttga  10260

cggcagcctg gggaggcagc tcggaagcct ggttgatctt gggcattcgc ttgagcttgt  10320

cgaaggcttc ctgagagtgc ataaccatga aggcgtcagt agcatctcgt ccctggtagt  10380

tctcaatgat ttcagctcca ccagggtgga agttcaccca agcggagacg tcgtacacct  10440

ttccgtcgat gacgaggggc agagcctgtc gagaagcctt caccatgggc aggacctgtg  10500

ttagtacatt gtcggggagt catcaattgg ttcgacaggt tgtcgactgt tagtatgagc  10560

tcaattgggc tctggtgggt cgatgacact tgtcatctgt ttctgttggg tcatgtttcc  10620

atcaccttct atggtactca caattcgtcc gattcgcccg aatccgttaa taccgacttt  10680

gatggccatg ttgatgtgtg tttaattcaa gaatgaatat agagaagaga agaagaaaaa  10740

agattcaatt gagccggcga tgcagaccct tatataaatg ttgccttgga cagacggagc  10800

aagcccgccc aaacctacgt tcggtataat atgttaagct ttttaacaca aaggtttggc  10860

ttggggtaac ctgatgtggt gcaaaagacc gggcgttggc gagccattgc gcgggcgaat  10920

ggggccgtga ctcgtctcaa attcgagggc gtgcctcaat tcgtgccccc gtggcttttt  10980

cccgccgttt ccgccccgtt tgcaccactg cagccgcttc tttggttcgg acaccttgct  11040

gcgagctagg tgccttgtgc tacttaaaaa gtggcctccc aacaccaaca tgacatgagt  11100

gcgtgggcca agacacgttg gcggggtcgc agtcggctca atggcccgga aaaaacgctg  11160

ctggagctgg ttcggacgca gtccgccgcg gcgtatggat atccgcaagg ttccatagcg  11220

ccattgccct ccgtcggcgt ctatcccgca acctctaaat agagcgggaa tataacccaa  11280

gcttcttttt tttcctttaa cacgcacacc cccaactatc atgttgctgc tgctgtttga  11340

ctctactctg tggaggggtg ctcccaccca acccaaccta caggtggatc cggcgctgtg  11400

attggctgat aagtctccta tccggactaa ttctgaccaa tgggacatgc gcgcaggacc  11460

caaatgccgc aattacgtaa ccccaacgaa atgcctaccc ctctttggag cccagcggcc  11520

ccaaatcccc ccaagcagcc cggttctacc ggcttccatc tccaagcaca agcagcccgg  11580

aattccttta cctgcaggat aacttcgtat aatgtatgct atacgaagtt atgatctctc  11640

tcttgagctt ttccataaca agttcttctg cctccaggaa gtccatgggt ggtttgatca  11700

tggttttggt gtagtggtag tgcagtggtg gtattgtgac tggggatgta gttgagaata  11760

agtcatacac aagtcagctt tcttcgagcc tcatataagt ataagtagtt caacgtatta  11820

gcactgtacc cagcatctcc gtatcgagaa acacaacaac atgccccatt ggacagatca  11880

tgcggataca caggttgtgc agtatcatac atactcgatc agacaggtcg tctgaccatc  11940

atacaagctg aacaagcgct ccatacttgc acgctctcta tatacacagt taaattacat  12000

atccatagtc taacctctaa cagttaatct tctggtaagc ctcccagcca gccttctggt  12060

atcgcttggc ctcctcaata ggatctcggt tctggccgta cagacctcgg ccgacaatta  12120

tgatatccgt tccggtagac atgacatcct caacagttcg gtactgctgt ccgagagcgt  12180

ctcccttgtc gtcaagaccc accccggggg tcagaataag ccagtcctca gagtcgccct  12240

taggtcggtt ctgggcaatg aagccaacca caaactcggg gtcggatcgg gcaagctcaa  12300

tggtctgctt ggagtactcg ccagtggcca gagagccctt gcaagacagc tcggccagca  12360

tgagcagacc tctggccagc ttctcgttgg gagaggggac taggaactcc ttgtactggg  12420

agttctcgta gtcagagacg tcctccttct tctgttcaga gacagtttcc tcggcaccag  12480

ctcgcaggcc agcaatgatt ccggttccgg gtacaccgtg ggcgttggtg atatcggacc  12540

actcggcgat tcggtgacac cggtactggt gcttgacagt gttgccaata tctgcgaact  12600

ttctgtcctc gaacaggaag aaaccgtgct taagagcaag ttccttgagg gggagcacag  12660

tgccggcgta ggtgaagtcg tcaatgatgt cgatatgggt tttgatcatg cacacataag  12720

gtccgacctt atcggcaagc tcaatgagct ccttggtggt ggtaacatcc agagaagcac  12780

acaggttggt tttcttggct gccacgagct tgagcactcg agcggcaaag gcggacttgt  12840

ggacgttagc tcgagcttcg taggagggca ttttggtggt gaagaggaga ctgaaataaa  12900

tttagtctgc agaacttttt atcggaacct tatctggggc agtgaagtat atgttatggt  12960

aatagttacg agttagttga acttatagat agactggact atacggctat cggtccaaat  13020

tagaaagaac gtcaatggct ctctgggcgt cgcctttgcc gacaaaaatg tgatcatgat  13080

gaaagccagc aatgacgttg cagctgatat tgttgtcggc caaccgcgcc gaaaacgcag  13140

ctgtcagacc cacagcctcc aacgaagaat gtatcgtcaa agtgatccaa gcacactcat  13200

agttggagtc gtactccaaa ggcggcaatg acgagtcaga cagatactcg tcgacgcgat  13260

aacttcgtat aatgtatgct atacgaagtt atcgtacgat agttagtaga caacaatcga  13320

taacgtctcg taccaaccac agattacgac ccattcgcag tcacagttca ctagggtttg  13380

ggttgcatcc gttgagagcg gtttgttttt aaccttctcc atgtgctcac tcaggttttg  13440

ggttcagatc aaatcaaggc gtgaaccact ttgtttgagg acaaatgtga cacaaccaac  13500

cagtgtcagg ggcaagtccg tgacaaaggg gaagatacaa tgcaattact gacagttaca  13560

gactgcctcg atgccctaac cttgccccaa aataagacaa ctgtcctcgt ttaagcgcaa  13620

ccctattcag cgtcacgtca taatagcgtt tggatagcac tagtctatga ggagcgtttt  13680

atgttgcggt gagggcgatt ggtgctcata tgggttcaat tgaggtggcg gaacgagctt  13740

agtcttcaat tgaggtgcga gcgacacaat tgggtgtcac gtggcctaat tgacctcggg  13800

tcgtggagtc cccagttata cagcaaccac gaggtgcatg ggtaggagac gtcaccagac  13860

aatagggttt tttttggact ggagagggtt gggcaaaagc gctcaacggg ctgtttgggg  13920

agctgtgggg gaggaattgg cgatatttgt gaggttaacg gctccgattt gcgtgttttg  13980

tcgctcctgc atctccccat acccatatct tccctcccca cctctttcca cgataatttt  14040

acggatcagc aataaggttc cttctcctag tttccacgtc catatatatc tatgctgcgt  14100

cgtccttttc gtgacatcac caaaacacat acaacaatgg ctgttactga cgtccttaag  14160

cgaaagtccg gtgtcatcgt cggcgacgat gtccgagccg tgagtatcca cgacaagatc  14220

agtgtcgaga cgacgcgttt tgtgtaatga cacaatccga aagtcgctag caacacacac  14280

tctctacaca aactaaccca gctctccatg gcctccacct cggctctgcc caagcagaac  14340

cctgccctcc gacgaaccgt cacttccacc actgtgaccg actcggagtc tgctgccgtc  14400

tctccctccg attctcccag acactcggcc tcctctacat cgctgtcttc catgtccgag  14460

gtggacattg ccaagcccaa gtccgagtac ggtgtcatgc tggataccta cggcaaccag  14520

ttcgaagttc ccgacttcac catcaaggac atctacaacg ctattcccaa gcactgcttc  14580

aagcgatctg ctctcaaggg atacggctac attcttcgag acattgtcct cctgactacc  14640

actttcagca tctggtacaa ctttgtgaca cccgagtaca ttccctccac tcctgctcga  14700

gccggtctgt gggctgtgta caccgttctt cagggactct tcggtactgg actgtgggtc  14760

attgcccacg agtgtggaca tggtgctttc tccgattccc gaatcatcaa cgacattact  14820

ggctgggtgc ttcactcttc cctgcttgtt ccctacttca gctggcaaat ctcccaccgg  14880

aagcatcaca aggccactgg aaacatggag cgagacatgg tcttcgttcc tcgaacccga  14940

gagcagcaag ctactcgact cggcaagatg acccacgaac tcgcccatct taccgaggaa  15000

actcctgctt tcaccctgct catgcttgtg cttcagcaac tggtcggttg gcccaactat  15060

ctcattacca acgttactgg acacaactac catgagcggc agcgagaggg tcgaggcaag  15120

ggaaagcaca acggtcttgg cggtggagtt aaccatttcg atccccgatc tcctctgtac  15180

gagaacagcg acgccaagct catcgtgctc tccgacattg gcattggtct tatggccacc  15240

gctctgtact ttctcgttca gaagttcgga ttctacaaca tggccatctg gtacttcgtt  15300

ccctacttgt gggttaacca ctggctcgtc gccattacct ttctgcagca cacagatcct  15360

actcttcccc actacaccaa cgacgagtgg aactttgtgc gaggtgccgc tgcaaccatc  15420

gaccgagaga tgggcttcat tggacgtcat ctgctccacg gcattatcga gactcacgtc  15480

ctgcatcact acgtctcttc cattcccttc tacaatgcgg acgaagctac cgaggccatc  15540

aaacctatca tgggcaagca ctatcgagct gatgtccagg acggtcctcg aggattcatt  15600

cgagccatgt accgatctgc acgaatgtgc cagtgggttg aaccctccgc tggtgccgag  15660

ggagctggca agggtgtcct gttctttcga aaccgaaaca atgtgggcac tcctcccgct  15720

gtcatcaagc ccgttgccta agc                                          15743


<210>  56
<211>  1434
<212>  DNA
<213>  Fusarium moniliforme 


<220>
<221>  CDS
<222>  (1)..(1434)
<223>  synthetic delta-12 desaturase (codon-optimized for Yarrowia 
       lipolytica)

<300>
<302>  DELTA-12 DESATURASES SUITABLE FOR ALTERING LEVELS OF 
       POLYUNSATURATED FATTY ACIDS IN OLEAGINOUS YEAST
<310>  WO 2005/047485
<311>  2004-11-12
<312>  2005-05-26
<313>  (1)..(1434)

<300>
<302>  DELTA-12 DESATURASES SUITABLE FOR ALTERING LEVELS OF 
       POLYUNSATURATED FATTY ACIDS IN OLEAGINOUS YEAST
<310>  US 2005-0216975-A1
<311>  2004-11-10
<312>  2005-09-29
<313>  (1)..(1434)

<400>  56
atg gcc tcc acc tcg gct ctg ccc aag cag aac cct gcc ctc cga cga       48
Met Ala Ser Thr Ser Ala Leu Pro Lys Gln Asn Pro Ala Leu Arg Arg         
1               5                   10                  15              

acc gtc act tcc acc act gtg acc gac tcg gag tct gct gcc gtc tct       96
Thr Val Thr Ser Thr Thr Val Thr Asp Ser Glu Ser Ala Ala Val Ser         
            20                  25                  30                  

ccc tcc gat tct ccc aga cac tcg gcc tcc tct aca tcg ctg tct tcc      144
Pro Ser Asp Ser Pro Arg His Ser Ala Ser Ser Thr Ser Leu Ser Ser         
        35                  40                  45                      

atg tcc gag gtg gac att gcc aag ccc aag tcc gag tac ggt gtc atg      192
Met Ser Glu Val Asp Ile Ala Lys Pro Lys Ser Glu Tyr Gly Val Met         
    50                  55                  60                          

ctg gat acc tac ggc aac cag ttc gaa gtt ccc gac ttc acc atc aag      240
Leu Asp Thr Tyr Gly Asn Gln Phe Glu Val Pro Asp Phe Thr Ile Lys         
65                  70                  75                  80          

gac atc tac aac gct att ccc aag cac tgc ttc aag cga tct gct ctc      288
Asp Ile Tyr Asn Ala Ile Pro Lys His Cys Phe Lys Arg Ser Ala Leu         
                85                  90                  95              

aag gga tac ggc tac att ctt cga gac att gtc ctc ctg act acc act      336
Lys Gly Tyr Gly Tyr Ile Leu Arg Asp Ile Val Leu Leu Thr Thr Thr         
            100                 105                 110                 

ttc agc atc tgg tac aac ttt gtg aca ccc gag tac att ccc tcc act      384
Phe Ser Ile Trp Tyr Asn Phe Val Thr Pro Glu Tyr Ile Pro Ser Thr         
        115                 120                 125                     

cct gct cga gcc ggt ctg tgg gct gtg tac acc gtt ctt cag gga ctc      432
Pro Ala Arg Ala Gly Leu Trp Ala Val Tyr Thr Val Leu Gln Gly Leu         
    130                 135                 140                         

ttc ggt act gga ctg tgg gtc att gcc cac gag tgt gga cat ggt gct      480
Phe Gly Thr Gly Leu Trp Val Ile Ala His Glu Cys Gly His Gly Ala         
145                 150                 155                 160         

ttc tcc gat tcc cga atc atc aac gac att act ggc tgg gtg ctt cac      528
Phe Ser Asp Ser Arg Ile Ile Asn Asp Ile Thr Gly Trp Val Leu His         
                165                 170                 175             

tct tcc ctg ctt gtt ccc tac ttc agc tgg caa atc tcc cac cgg aag      576
Ser Ser Leu Leu Val Pro Tyr Phe Ser Trp Gln Ile Ser His Arg Lys         
            180                 185                 190                 

cat cac aag gcc act gga aac atg gag cga gac atg gtc ttc gtt cct      624
His His Lys Ala Thr Gly Asn Met Glu Arg Asp Met Val Phe Val Pro         
        195                 200                 205                     

cga acc cga gag cag caa gct act cga ctc ggc aag atg acc cac gaa      672
Arg Thr Arg Glu Gln Gln Ala Thr Arg Leu Gly Lys Met Thr His Glu         
    210                 215                 220                         

ctc gcc cat ctt acc gag gaa act cct gct ttc acc ctg ctc atg ctt      720
Leu Ala His Leu Thr Glu Glu Thr Pro Ala Phe Thr Leu Leu Met Leu         
225                 230                 235                 240         

gtg ctt cag caa ctg gtc ggt tgg ccc aac tat ctc att acc aac gtt      768
Val Leu Gln Gln Leu Val Gly Trp Pro Asn Tyr Leu Ile Thr Asn Val         
                245                 250                 255             

act gga cac aac tac cat gag cgg cag cga gag ggt cga ggc aag gga      816
Thr Gly His Asn Tyr His Glu Arg Gln Arg Glu Gly Arg Gly Lys Gly         
            260                 265                 270                 

aag cac aac ggt ctt ggc ggt gga gtt aac cat ttc gat ccc cga tct      864
Lys His Asn Gly Leu Gly Gly Gly Val Asn His Phe Asp Pro Arg Ser         
        275                 280                 285                     

cct ctg tac gag aac agc gac gcc aag ctc atc gtg ctc tcc gac att      912
Pro Leu Tyr Glu Asn Ser Asp Ala Lys Leu Ile Val Leu Ser Asp Ile         
    290                 295                 300                         

ggc att ggt ctt atg gcc acc gct ctg tac ttt ctc gtt cag aag ttc      960
Gly Ile Gly Leu Met Ala Thr Ala Leu Tyr Phe Leu Val Gln Lys Phe         
305                 310                 315                 320         

gga ttc tac aac atg gcc atc tgg tac ttc gtt ccc tac ttg tgg gtt     1008
Gly Phe Tyr Asn Met Ala Ile Trp Tyr Phe Val Pro Tyr Leu Trp Val         
                325                 330                 335             

aac cac tgg ctc gtc gcc att acc ttt ctg cag cac aca gat cct act     1056
Asn His Trp Leu Val Ala Ile Thr Phe Leu Gln His Thr Asp Pro Thr         
            340                 345                 350                 

ctt ccc cac tac acc aac gac gag tgg aac ttt gtg cga ggt gcc gct     1104
Leu Pro His Tyr Thr Asn Asp Glu Trp Asn Phe Val Arg Gly Ala Ala         
        355                 360                 365                     

gca acc atc gac cga gag atg ggc ttc att gga cgt cat ctg ctc cac     1152
Ala Thr Ile Asp Arg Glu Met Gly Phe Ile Gly Arg His Leu Leu His         
    370                 375                 380                         

ggc att atc gag act cac gtc ctg cat cac tac gtc tct tcc att ccc     1200
Gly Ile Ile Glu Thr His Val Leu His His Tyr Val Ser Ser Ile Pro         
385                 390                 395                 400         

ttc tac aat gcg gac gaa gct acc gag gcc atc aaa cct atc atg ggc     1248
Phe Tyr Asn Ala Asp Glu Ala Thr Glu Ala Ile Lys Pro Ile Met Gly         
                405                 410                 415             

aag cac tat cga gct gat gtc cag gac ggt cct cga gga ttc att cga     1296
Lys His Tyr Arg Ala Asp Val Gln Asp Gly Pro Arg Gly Phe Ile Arg         
            420                 425                 430                 

gcc atg tac cga tct gca cga atg tgc cag tgg gtt gaa ccc tcc gct     1344
Ala Met Tyr Arg Ser Ala Arg Met Cys Gln Trp Val Glu Pro Ser Ala         
        435                 440                 445                     

ggt gcc gag gga gct ggc aag ggt gtc ctg ttc ttt cga aac cga aac     1392
Gly Ala Glu Gly Ala Gly Lys Gly Val Leu Phe Phe Arg Asn Arg Asn         
    450                 455                 460                         

aat gtg ggc act cct ccc gct gtc atc aag ccc gtt gcc taa             1434
Asn Val Gly Thr Pro Pro Ala Val Ile Lys Pro Val Ala                     
465                 470                 475                             


<210>  57
<211>  477
<212>  PRT
<213>  Fusarium moniliforme 

<400>  57

Met Ala Ser Thr Ser Ala Leu Pro Lys Gln Asn Pro Ala Leu Arg Arg 
1               5                   10                  15      


Thr Val Thr Ser Thr Thr Val Thr Asp Ser Glu Ser Ala Ala Val Ser 
            20                  25                  30          


Pro Ser Asp Ser Pro Arg His Ser Ala Ser Ser Thr Ser Leu Ser Ser 
        35                  40                  45              


Met Ser Glu Val Asp Ile Ala Lys Pro Lys Ser Glu Tyr Gly Val Met 
    50                  55                  60                  


Leu Asp Thr Tyr Gly Asn Gln Phe Glu Val Pro Asp Phe Thr Ile Lys 
65                  70                  75                  80  


Asp Ile Tyr Asn Ala Ile Pro Lys His Cys Phe Lys Arg Ser Ala Leu 
                85                  90                  95      


Lys Gly Tyr Gly Tyr Ile Leu Arg Asp Ile Val Leu Leu Thr Thr Thr 
            100                 105                 110         


Phe Ser Ile Trp Tyr Asn Phe Val Thr Pro Glu Tyr Ile Pro Ser Thr 
        115                 120                 125             


Pro Ala Arg Ala Gly Leu Trp Ala Val Tyr Thr Val Leu Gln Gly Leu 
    130                 135                 140                 


Phe Gly Thr Gly Leu Trp Val Ile Ala His Glu Cys Gly His Gly Ala 
145                 150                 155                 160 


Phe Ser Asp Ser Arg Ile Ile Asn Asp Ile Thr Gly Trp Val Leu His 
                165                 170                 175     


Ser Ser Leu Leu Val Pro Tyr Phe Ser Trp Gln Ile Ser His Arg Lys 
            180                 185                 190         


His His Lys Ala Thr Gly Asn Met Glu Arg Asp Met Val Phe Val Pro 
        195                 200                 205             


Arg Thr Arg Glu Gln Gln Ala Thr Arg Leu Gly Lys Met Thr His Glu 
    210                 215                 220                 


Leu Ala His Leu Thr Glu Glu Thr Pro Ala Phe Thr Leu Leu Met Leu 
225                 230                 235                 240 


Val Leu Gln Gln Leu Val Gly Trp Pro Asn Tyr Leu Ile Thr Asn Val 
                245                 250                 255     


Thr Gly His Asn Tyr His Glu Arg Gln Arg Glu Gly Arg Gly Lys Gly 
            260                 265                 270         


Lys His Asn Gly Leu Gly Gly Gly Val Asn His Phe Asp Pro Arg Ser 
        275                 280                 285             


Pro Leu Tyr Glu Asn Ser Asp Ala Lys Leu Ile Val Leu Ser Asp Ile 
    290                 295                 300                 


Gly Ile Gly Leu Met Ala Thr Ala Leu Tyr Phe Leu Val Gln Lys Phe 
305                 310                 315                 320 


Gly Phe Tyr Asn Met Ala Ile Trp Tyr Phe Val Pro Tyr Leu Trp Val 
                325                 330                 335     


Asn His Trp Leu Val Ala Ile Thr Phe Leu Gln His Thr Asp Pro Thr 
            340                 345                 350         


Leu Pro His Tyr Thr Asn Asp Glu Trp Asn Phe Val Arg Gly Ala Ala 
        355                 360                 365             


Ala Thr Ile Asp Arg Glu Met Gly Phe Ile Gly Arg His Leu Leu His 
    370                 375                 380                 


Gly Ile Ile Glu Thr His Val Leu His His Tyr Val Ser Ser Ile Pro 
385                 390                 395                 400 


Phe Tyr Asn Ala Asp Glu Ala Thr Glu Ala Ile Lys Pro Ile Met Gly 
                405                 410                 415     


Lys His Tyr Arg Ala Asp Val Gln Asp Gly Pro Arg Gly Phe Ile Arg 
            420                 425                 430         


Ala Met Tyr Arg Ser Ala Arg Met Cys Gln Trp Val Glu Pro Ser Ala 
        435                 440                 445             


Gly Ala Glu Gly Ala Gly Lys Gly Val Leu Phe Phe Arg Asn Arg Asn 
    450                 455                 460                 


Asn Val Gly Thr Pro Pro Ala Val Ile Lys Pro Val Ala 
465                 470                 475         


<210>  58
<211>  6303
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZKUE3S

<400>  58
ggccgcaagt gtggatgggg aagtgagtgc ccggttctgt gtgcacaatt ggcaatccaa     60

gatggatgga ttcaacacag ggatatagcg agctacgtgg tggtgcgagg atatagcaac    120

ggatatttat gtttgacact tgagaatgta cgatacaagc actgtccaag tacaatacta    180

aacatactgt acatactcat actcgtaccc gggcaacggt ttcacttgag tgcagtggct    240

agtgctctta ctcgtacagt gtgcaatact gcgtatcata gtctttgatg tatatcgtat    300

tcattcatgt tagttgcgta cgaggaaact gtctctgaac agaagaagga ggacgtctct    360

gactacgaga actcccagta caaggagttc ctagtcccct ctcccaacga gaagctggcc    420

agaggtctgc tcatgctggc cgagctgtct tgcaagggct ctctggccac tggcgagtac    480

tccaagcaga ccattgagct tgcccgatcc gaccccgagt ttgtggttgg cttcattgcc    540

cagaaccgac ctaagggcga ctctgaggac tggcttattc tgacccccgg ggtgggtctt    600

gacgacaagg gagacgctct cggacagcag taccgaactg ttgaggatgt catgtctacc    660

ggaacggata tcataattgt cggccgaggt ctgtacggcc agaaccgaga tcctattgag    720

gaggccaagc gataccagaa ggctggctgg gaggcttacc agaagattaa ctgttagagg    780

ttagactatg gatatgtaat ttaactgtgt atatagagag cgtgcaagta tggagcgctt    840

gttcagcttg tatgatggtc agacgacctg tctgatcgag tatgtatgat actgcacaac    900

ctgtgtatcc gcatgatctg tccaatgggg catgttgttg tgtttctcga tacggagatg    960

ctgggtacag tgctaatacg ttgaactact tatacttata tgaggctcga agaaagctga   1020

cttgtgtatg acttaattaa tcgagcttgg cgtaatcatg gtcatagctg tttcctgtgt   1080

gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag   1140

cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt   1200

tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag   1260

gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg   1320

ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat   1380

caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta   1440

aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa   1500

atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc   1560

cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt   1620

ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca   1680

gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg   1740

accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat   1800

cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta   1860

cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct   1920

gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac   1980

aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa   2040

aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa   2100

actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt   2160

taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca   2220

gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca   2280

tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc   2340

ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa   2400

accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc   2460

agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca   2520

acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat   2580

tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag   2640

cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac   2700

tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt   2760

ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt   2820

gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc   2880

tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat   2940

ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca   3000

gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga   3060

cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg   3120

gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg   3180

ttccgcgcac atttccccga aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg   3240

cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg   3300

ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc   3360

taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa   3420

aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc   3480

ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac   3540

tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt   3600

ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc   3660

ttacaatttc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc   3720

ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt   3780

aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat tgtaatacga   3840

ctcactatag ggcgaattgg gtaccgggcc ccccctcgag gtcgacgagt atctgtctga   3900

ctcgtcattg catgcctttg gagtacgact ccaactatga gtgtgcttgg atcactttga   3960

cgatacattc ttcgttggag gctgtgggtc tgacagctgc gttttcggcg cggttggccg   4020

acaacaatat cagctgcaac gtcattgctg gctttcatca tgatcacatt tttgtcggca   4080

aaggcgacgc ccagagagcc attgacgttc tttctaattt ggaccgatag ccgtatagtc   4140

cagtctatct ataagttcaa ctaactcgta actattacca taacatatac ttcactgccc   4200

cagataaggt tccgataaaa agttctgcag actaaattta tttcagtctc ctcttcacca   4260

ccaaaatgcc ctcctacgaa gctcgagtgc tcaagctcgt ggcagccaag aaaaccaacc   4320

tgtgtgcttc tctggatgtt accaccacca aggagctcat tgagcttgcc gataaggtcg   4380

gaccttatgt gtgcatgatc aaaacccata tcgacatcat tgacgacttc acctacgccg   4440

gcactgtgct ccccctcaag gaacttgctc ttaagcacgg tttcttcctg ttcgaggaca   4500

gaaagttcgc agatattggc aacactgtca agcaccagta ccggtgtcac cgaatcgccg   4560

agtggtccga tatcaccaac gcccacggtg tttaaacccg gaaccggaat cgataagctt   4620

gatatcgaat tcatgctgtt catcgtggtt aatgctgctg tgtgctgtgt gtgtgtgttg   4680

tttggcgctc attgttgcgt tatgcagcgt acaccacaat attggaagct tattagcctt   4740

tctatttttt cgtttgcaag gcttaacaac attgctgtgg agagggatgg ggatatggag   4800

gccgctggag ggagtcggag aggcgttttg gagcggcttg gcctggcgcc cagctcgcga   4860

aacgcaccta ggaccctttg gcacgccgaa atgtgccact tttcagtcta gtaacgcctt   4920

acctacgtca ttccatgcgt gcatgtttgc gccttttttc ccttgccctt gatcgccaca   4980

cagtacagtg cactgtacag tggaggtttt gggggggtct tagatgggag ctaaaagcgg   5040

cctagcggta cactagtggg attgtatgga gtggcatgga gcctaggtgg agcctgacag   5100

gacgcacgac cggctagccc gtgacagacg atgggtggct cctgttgtcc accgcgtaca   5160

aatgtttggg ccaaagtctt gtcagccttg cttgcgaacc taattcccaa ttttgtcact   5220

tcgcaccccc attgatcgag ccctaacccc tgcccatcag gcaatccaat taagctcgca   5280

ttgtctgcct tgtttagttt ggctcctgcc cgtttcggcg tccacttgca caaacacaaa   5340

caagcattat atataaggct cgtctctccc tcccaaccac actcactttt ttgcccgtct   5400

tcccttgcta acacaaaagt caagaacaca aacaaccacc ccaaccccct tacacacaag   5460

acatatctac accatggagt ctggacccat gcctgctggc attcccttcc ctgagtacta   5520

tgacttcttt atggactgga agactcccct ggccatcgct gccacctaca ctgctgccgt   5580

cggtctcttc aaccccaagg ttggcaaggt ctcccgagtg gttgccaagt cggctaacgc   5640

aaagcctgcc gagcgaaccc agtccggagc tgccatgact gccttcgtct ttgtgcacaa   5700

cctcattctg tgtgtctact ctggcatcac cttctactac atgtttcctg ctatggtcaa   5760

gaacttccga acccacacac tgcacgaagc ctactgcgac acggatcagt ccctctggaa   5820

caacgcactt ggctactggg gttacctctt ctacctgtcc aagttctacg aggtcattga   5880

caccatcatc atcatcctga agggacgacg gtcctcgctg cttcagacct accaccatgc   5940

tggagccatg attaccatgt ggtctggcat caactaccaa gccactccca tttggatctt   6000

tgtggtcttc aactccttca ttcacaccat catgtactgt tactatgcct tcacctctat   6060

cggattccat cctcctggca aaaagtacct gacttcgatg cagattactc agtttctggt   6120

cggtatcacc attgccgtgt cctacctctt cgttcctggc tgcatccgaa cacccggtgc   6180

tcagatggct gtctggatca acgtcggcta cctgtttccc ttgacctatc tgttcgtgga   6240

ctttgccaag cgaacctact ccaagcgatc tgccattgcc gctcagaaaa aggctcagta   6300

agc                                                                 6303


<210>  59
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer pZP-GW-5-1

<400>  59
cgacaagatg gaatgagaat g                                               21


<210>  60
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer pZP-GW-5-2

<400>  60
ctggtttttc aactacttct ac                                              22


<210>  61
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer pZP-GW-5-3

<400>  61
gtactgtcct gtgtctgttc c                                               21


<210>  62
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer pZP-GW-5-4

<400>  62
ctacatcgtc cgaaagcaca ag                                              22


<210>  63
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer pZP-GW-3-1

<400>  63
ctaccagatc gagcaccatc tctg                                            24


<210>  64
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer pZP-GW-3-2

<400>  64
ctaccaggtg gaacagctgt g                                               21


<210>  65
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer pZP-GW-3-3

<400>  65
tctgccccat gaaggtctcg tc                                              22


<210>  66
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer pZP-GW-3-4

<400>  66
cctgtcccag ttcgctcgaa tg                                              22


<210>  67
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Genome Walker adaptor-1

<400>  67
gtaatacgac tatagggcac gcgtggtcga cggcccgggc tggt                      44


<210>  68
<211>  8
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Genome Walker adaptor-2


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5' end is associated with a -PO4 group

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  3' end is associated with a -H2N group

<400>  68
accagccc                                                               8


<210>  69
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nested adaptor primer

<400>  69
gtaatacgac tcactatagg gc                                              22


<210>  70
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Per10F1

<400>  70
gatcaaccat ggggggaagt tcacatgcat tcgctg                               36


<210>  71
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer ZPGW-5-5

<400>  71
gttatagttt tcatgtgaaa taccgagag                                       29


<210>  72
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Per10R

<400>  72
gatcaagcgg ccgccagacc tcgtcattat ctgatag                              37


<210>  73
<211>  7222
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pFBAIn-MOD-1

<400>  73
catggatcca ggcctgttaa cggccattac ggcctgcagg atccgaaaaa acctcccaca     60

cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc    120

agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt    180

ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgcgg    240

ccgcaagtgt ggatggggaa gtgagtgccc ggttctgtgt gcacaattgg caatccaaga    300

tggatggatt caacacaggg atatagcgag ctacgtggtg gtgcgaggat atagcaacgg    360

atatttatgt ttgacacttg agaatgtacg atacaagcac tgtccaagta caatactaaa    420

catactgtac atactcatac tcgtacccgg gcaacggttt cacttgagtg cagtggctag    480

tgctcttact cgtacagtgt gcaatactgc gtatcatagt ctttgatgta tatcgtattc    540

attcatgtta gttgcgtacg agccggaagc ataaagtgta aagcctgggg tgcctaatga    600

gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg    660

tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg    720

cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg    780

gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga    840

aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg    900

gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag    960

aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc   1020

gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg   1080

ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt   1140

cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc   1200

ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc   1260

actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg   1320

tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca   1380

gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc   1440

ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat   1500

cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt   1560

ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt   1620

tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc   1680

agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc   1740

gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata   1800

ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg   1860

gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc   1920

cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct   1980

acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa   2040

cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt   2100

cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca   2160

ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac   2220

tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca   2280

atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt   2340

tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc   2400

actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca   2460

aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata   2520

ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc   2580

ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc   2640

cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt   2700

acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc   2760

ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct   2820

ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat   2880

ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc   2940

acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc   3000

tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg   3060

atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttccattcgc   3120

cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc   3180

agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc   3240

agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat   3300

tgggtaccgg gccccccctc gaggtcgatg gtgtcgataa gcttgatatc gaattcatgt   3360

cacacaaacc gatcttcgcc tcaaggaaac ctaattctac atccgagaga ctgccgagat   3420

ccagtctaca ctgattaatt ttcgggccaa taatttaaaa aaatcgtgtt atataatatt   3480

atatgtatta tatatataca tcatgatgat actgacagtc atgtcccatt gctaaataga   3540

cagactccat ctgccgcctc caactgatgt tctcaatatt taaggggtca tctcgcattg   3600

tttaataata aacagactcc atctaccgcc tccaaatgat gttctcaaaa tatattgtat   3660

gaacttattt ttattactta gtattattag acaacttact tgctttatga aaaacacttc   3720

ctatttagga aacaatttat aatggcagtt cgttcattta acaatttatg tagaataaat   3780

gttataaatg cgtatgggaa atcttaaata tggatagcat aaatgatatc tgcattgcct   3840

aattcgaaat caacagcaac gaaaaaaatc ccttgtacaa cataaatagt catcgagaaa   3900

tatcaactat caaagaacag ctattcacac gttactattg agattattat tggacgagaa   3960

tcacacactc aactgtcttt ctctcttcta gaaatacagg tacaagtatg tactattctc   4020

attgttcata cttctagtca tttcatccca catattcctt ggatttctct ccaatgaatg   4080

acattctatc ttgcaaattc aacaattata ataagatata ccaaagtagc ggtatagtgg   4140

caatcaaaaa gcttctctgg tgtgcttctc gtatttattt ttattctaat gatccattaa   4200

aggtatatat ttatttcttg ttatataatc cttttgttta ttacatgggc tggatacata   4260

aaggtatttt gatttaattt tttgcttaaa ttcaatcccc cctcgttcag tgtcaactgt   4320

aatggtagga aattaccata cttttgaaga agcaaaaaaa atgaaagaaa aaaaaaatcg   4380

tatttccagg ttagacgttc cgcagaatct agaatgcggt atgcggtaca ttgttcttcg   4440

aacgtaaaag ttgcgctccc tgagatattg tacatttttg cttttacaag tacaagtaca   4500

tcgtacaact atgtactact gttgatgcat ccacaacagt ttgttttgtt tttttttgtt   4560

tttttttttt ctaatgattc attaccgcta tgtataccta cttgtacttg tagtaagccg   4620

ggttattggc gttcaattaa tcatagactt atgaatctgc acggtgtgcg ctgcgagtta   4680

cttttagctt atgcatgcta cttgggtgta atattgggat ctgttcggaa atcaacggat   4740

gctcaatcga tttcgacagt aattaattaa gtcatacaca agtcagcttt cttcgagcct   4800

catataagta taagtagttc aacgtattag cactgtaccc agcatctccg tatcgagaaa   4860

cacaacaaca tgccccattg gacagatcat gcggatacac aggttgtgca gtatcataca   4920

tactcgatca gacaggtcgt ctgaccatca tacaagctga acaagcgctc catacttgca   4980

cgctctctat atacacagtt aaattacata tccatagtct aacctctaac agttaatctt   5040

ctggtaagcc tcccagccag ccttctggta tcgcttggcc tcctcaatag gatctcggtt   5100

ctggccgtac agacctcggc cgacaattat gatatccgtt ccggtagaca tgacatcctc   5160

aacagttcgg tactgctgtc cgagagcgtc tcccttgtcg tcaagaccca ccccgggggt   5220

cagaataagc cagtcctcag agtcgccctt aggtcggttc tgggcaatga agccaaccac   5280

aaactcgggg tcggatcggg caagctcaat ggtctgcttg gagtactcgc cagtggccag   5340

agagcccttg caagacagct cggccagcat gagcagacct ctggccagct tctcgttggg   5400

agaggggact aggaactcct tgtactggga gttctcgtag tcagagacgt cctccttctt   5460

ctgttcagag acagtttcct cggcaccagc tcgcaggcca gcaatgattc cggttccggg   5520

tacaccgtgg gcgttggtga tatcggacca ctcggcgatt cggtgacacc ggtactggtg   5580

cttgacagtg ttgccaatat ctgcgaactt tctgtcctcg aacaggaaga aaccgtgctt   5640

aagagcaagt tccttgaggg ggagcacagt gccggcgtag gtgaagtcgt caatgatgtc   5700

gatatgggtt ttgatcatgc acacataagg tccgacctta tcggcaagct caatgagctc   5760

cttggtggtg gtaacatcca gagaagcaca caggttggtt ttcttggctg ccacgagctt   5820

gagcactcga gcggcaaagg cggacttgtg gacgttagct cgagcttcgt aggagggcat   5880

tttggtggtg aagaggagac tgaaataaat ttagtctgca gaacttttta tcggaacctt   5940

atctggggca gtgaagtata tgttatggta atagttacga gttagttgaa cttatagata   6000

gactggacta tacggctatc ggtccaaatt agaaagaacg tcaatggctc tctgggcgtc   6060

gcctttgccg acaaaaatgt gatcatgatg aaagccagca atgacgttgc agctgatatt   6120

gttgtcggcc aaccgcgccg aaaacgcagc tgtcagaccc acagcctcca acgaagaatg   6180

tatcgtcaaa gtgatccaag cacactcata gttggagtcg tactccaaag gcggcaatga   6240

cgagtcagac agatactcgt cgaaaacagt gtacgcagat ctactataga ggaacattta   6300

aattgccccg gagaagacgg ccaggccgcc tagatgacaa attcaacaac tcacagctga   6360

ctttctgcca ttgccactag gggggggcct ttttatatgg ccaagccaag ctctccacgt   6420

cggttgggct gcacccaaca ataaatgggt agggttgcac caacaaaggg atgggatggg   6480

gggtagaaga tacgaggata acggggctca atggcacaaa taagaacgaa tactgccatt   6540

aagactcgtg atccagcgac tgacaccatt gcatcatcta agggcctcaa aactacctcg   6600

gaactgctgc gctgatctgg acaccacaga ggttccgagc actttaggtt gcaccaaatg   6660

tcccaccagg tgcaggcaga aaacgctgga acagcgtgta cagtttgtct taacaaaaag   6720

tgagggcgct gaggtcgagc agggtggtgt gacttgttat agcctttaga gctgcgaaag   6780

cgcgtatgga tttggctcat caggccagat tgagggtctg tggacacatg tcatgttagt   6840

gtacttcaat cgccccctgg atatagcccc gacaataggc cgtggcctca tttttttgcc   6900

ttccgcacat ttccattgct cggtacccac accttgcttc tcctgcactt gccaacctta   6960

atactggttt acattgacca acatcttaca agcggggggc ttgtctaggg tatatataaa   7020

cagtggctct cccaatcggt tgccagtctc ttttttcctt tctttcccca cagattcgaa   7080

atctaaacta cacatcacag aattccgagc cgtgagtatc cacgacaaga tcagtgtcga   7140

gacgacgcgt tttgtgtaat gacacaatcc gaaagtcgct agcaacacac actctctaca   7200

caaactaacc cagctctggt ac                                            7222


<210>  74
<211>  8133
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pFBAIN-Pex10

<400>  74
ggccgcaagt gtggatgggg aagtgagtgc ccggttctgt gtgcacaatt ggcaatccaa     60

gatggatgga ttcaacacag ggatatagcg agctacgtgg tggtgcgagg atatagcaac    120

ggatatttat gtttgacact tgagaatgta cgatacaagc actgtccaag tacaatacta    180

aacatactgt acatactcat actcgtaccc gggcaacggt ttcacttgag tgcagtggct    240

agtgctctta ctcgtacagt gtgcaatact gcgtatcata gtctttgatg tatatcgtat    300

tcattcatgt tagttgcgta cgagccggaa gcataaagtg taaagcctgg ggtgcctaat    360

gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc    420

tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg    480

ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag    540

cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag    600

gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc    660

tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc    720

agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc    780

tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt    840

cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg    900

ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat    960

ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag   1020

ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt   1080

ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc   1140

cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta   1200

gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag   1260

atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga   1320

ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa   1380

gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa   1440

tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc   1500

ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga   1560

taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa   1620

gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt   1680

gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg   1740

ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc   1800

aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg   1860

gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag   1920

cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt   1980

actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt   2040

caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac   2100

gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac   2160

ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag   2220

caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa   2280

tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga   2340

gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc   2400

cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg   2460

ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct   2520

tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc   2580

ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg   2640

atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt   2700

ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg   2760

tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc   2820

tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttccattc   2880

gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg   2940

ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc   3000

ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga   3060

attgggtacc gggccccccc tcgaggtcga tggtgtcgat aagcttgata tcgaattcat   3120

gtcacacaaa ccgatcttcg cctcaaggaa acctaattct acatccgaga gactgccgag   3180

atccagtcta cactgattaa ttttcgggcc aataatttaa aaaaatcgtg ttatataata   3240

ttatatgtat tatatatata catcatgatg atactgacag tcatgtccca ttgctaaata   3300

gacagactcc atctgccgcc tccaactgat gttctcaata tttaaggggt catctcgcat   3360

tgtttaataa taaacagact ccatctaccg cctccaaatg atgttctcaa aatatattgt   3420

atgaacttat ttttattact tagtattatt agacaactta cttgctttat gaaaaacact   3480

tcctatttag gaaacaattt ataatggcag ttcgttcatt taacaattta tgtagaataa   3540

atgttataaa tgcgtatggg aaatcttaaa tatggatagc ataaatgata tctgcattgc   3600

ctaattcgaa atcaacagca acgaaaaaaa tcccttgtac aacataaata gtcatcgaga   3660

aatatcaact atcaaagaac agctattcac acgttactat tgagattatt attggacgag   3720

aatcacacac tcaactgtct ttctctcttc tagaaataca ggtacaagta tgtactattc   3780

tcattgttca tacttctagt catttcatcc cacatattcc ttggatttct ctccaatgaa   3840

tgacattcta tcttgcaaat tcaacaatta taataagata taccaaagta gcggtatagt   3900

ggcaatcaaa aagcttctct ggtgtgcttc tcgtatttat ttttattcta atgatccatt   3960

aaaggtatat atttatttct tgttatataa tccttttgtt tattacatgg gctggataca   4020

taaaggtatt ttgatttaat tttttgctta aattcaatcc cccctcgttc agtgtcaact   4080

gtaatggtag gaaattacca tacttttgaa gaagcaaaaa aaatgaaaga aaaaaaaaat   4140

cgtatttcca ggttagacgt tccgcagaat ctagaatgcg gtatgcggta cattgttctt   4200

cgaacgtaaa agttgcgctc cctgagatat tgtacatttt tgcttttaca agtacaagta   4260

catcgtacaa ctatgtacta ctgttgatgc atccacaaca gtttgttttg tttttttttg   4320

tttttttttt ttctaatgat tcattaccgc tatgtatacc tacttgtact tgtagtaagc   4380

cgggttattg gcgttcaatt aatcatagac ttatgaatct gcacggtgtg cgctgcgagt   4440

tacttttagc ttatgcatgc tacttgggtg taatattggg atctgttcgg aaatcaacgg   4500

atgctcaatc gatttcgaca gtaattaatt aagtcataca caagtcagct ttcttcgagc   4560

ctcatataag tataagtagt tcaacgtatt agcactgtac ccagcatctc cgtatcgaga   4620

aacacaacaa catgccccat tggacagatc atgcggatac acaggttgtg cagtatcata   4680

catactcgat cagacaggtc gtctgaccat catacaagct gaacaagcgc tccatacttg   4740

cacgctctct atatacacag ttaaattaca tatccatagt ctaacctcta acagttaatc   4800

ttctggtaag cctcccagcc agccttctgg tatcgcttgg cctcctcaat aggatctcgg   4860

ttctggccgt acagacctcg gccgacaatt atgatatccg ttccggtaga catgacatcc   4920

tcaacagttc ggtactgctg tccgagagcg tctcccttgt cgtcaagacc caccccgggg   4980

gtcagaataa gccagtcctc agagtcgccc ttaggtcggt tctgggcaat gaagccaacc   5040

acaaactcgg ggtcggatcg ggcaagctca atggtctgct tggagtactc gccagtggcc   5100

agagagccct tgcaagacag ctcggccagc atgagcagac ctctggccag cttctcgttg   5160

ggagagggga ctaggaactc cttgtactgg gagttctcgt agtcagagac gtcctccttc   5220

ttctgttcag agacagtttc ctcggcacca gctcgcaggc cagcaatgat tccggttccg   5280

ggtacaccgt gggcgttggt gatatcggac cactcggcga ttcggtgaca ccggtactgg   5340

tgcttgacag tgttgccaat atctgcgaac tttctgtcct cgaacaggaa gaaaccgtgc   5400

ttaagagcaa gttccttgag ggggagcaca gtgccggcgt aggtgaagtc gtcaatgatg   5460

tcgatatggg ttttgatcat gcacacataa ggtccgacct tatcggcaag ctcaatgagc   5520

tccttggtgg tggtaacatc cagagaagca cacaggttgg ttttcttggc tgccacgagc   5580

ttgagcactc gagcggcaaa ggcggacttg tggacgttag ctcgagcttc gtaggagggc   5640

attttggtgg tgaagaggag actgaaataa atttagtctg cagaactttt tatcggaacc   5700

ttatctgggg cagtgaagta tatgttatgg taatagttac gagttagttg aacttataga   5760

tagactggac tatacggcta tcggtccaaa ttagaaagaa cgtcaatggc tctctgggcg   5820

tcgcctttgc cgacaaaaat gtgatcatga tgaaagccag caatgacgtt gcagctgata   5880

ttgttgtcgg ccaaccgcgc cgaaaacgca gctgtcagac ccacagcctc caacgaagaa   5940

tgtatcgtca aagtgatcca agcacactca tagttggagt cgtactccaa aggcggcaat   6000

gacgagtcag acagatactc gtcgaaaaca gtgtacgcag atctactata gaggaacatt   6060

taaattgccc cggagaagac ggccaggccg cctagatgac aaattcaaca actcacagct   6120

gactttctgc cattgccact aggggggggc ctttttatat ggccaagcca agctctccac   6180

gtcggttggg ctgcacccaa caataaatgg gtagggttgc accaacaaag ggatgggatg   6240

gggggtagaa gatacgagga taacggggct caatggcaca aataagaacg aatactgcca   6300

ttaagactcg tgatccagcg actgacacca ttgcatcatc taagggcctc aaaactacct   6360

cggaactgct gcgctgatct ggacaccaca gaggttccga gcactttagg ttgcaccaaa   6420

tgtcccacca ggtgcaggca gaaaacgctg gaacagcgtg tacagtttgt cttaacaaaa   6480

agtgagggcg ctgaggtcga gcagggtggt gtgacttgtt atagccttta gagctgcgaa   6540

agcgcgtatg gatttggctc atcaggccag attgagggtc tgtggacaca tgtcatgtta   6600

gtgtacttca atcgccccct ggatatagcc ccgacaatag gccgtggcct catttttttg   6660

ccttccgcac atttccattg ctcggtaccc acaccttgct tctcctgcac ttgccaacct   6720

taatactggt ttacattgac caacatctta caagcggggg gcttgtctag ggtatatata   6780

aacagtggct ctcccaatcg gttgccagtc tcttttttcc tttctttccc cacagattcg   6840

aaatctaaac tacacatcac agaattccga gccgtgagta tccacgacaa gatcagtgtc   6900

gagacgacgc gttttgtgta atgacacaat ccgaaagtcg ctagcaacac acactctcta   6960

cacaaactaa cccagctctg gtaccatggg gggaagttca catgcattcg ctggtgaatc   7020

tgatctgaca ctacaactac acaccaggtc caacatgagc gacaatacga caatcaaaaa   7080

gccgatccga cccaaaccga tccggacgga acgcctgcct tacgctgggg ccgcagaaat   7140

catccgagcc aaccagaaag accactactt tgagtccgtg cttgaacagc atctcgtcac   7200

gtttctgcag aaatggaagg gagtacgatt tatccaccag tacaaggagg agctggagac   7260

ggcgtccaag tttgcatatc tcggtttgtg tacgcttgtg ggctccaaga ctctcggaga   7320

agagtacacc aatctcatgt acactatcag agaccgaaca gctctaccgg gggtggtgag   7380

acggtttggc tacgtgcttt ccaacactct gtttccatac ctgtttgtgc gctacatggg   7440

caagttgcgc gccaaactga tgcgcgagta tccccatctg gtggagtacg acgaagatga   7500

gcctgtgccc agcccggaaa catggaagga gcgggtcatc aagacgtttg tgaacaagtt   7560

tgacaagttc acggcgctgg aggggtttac cgcgatccac ttggcgattt tctacgtcta   7620

cggctcgtac taccagctca gtaagcggat ctggggcatg cgttatgtat ttggacaccg   7680

actggacaag aatgagcctc gaatcggtta cgagatgctc ggtctgctga ttttcgcccg   7740

gtttgccacg tcatttgtgc agacgggaag agagtacctc ggagcgctgc tggaaaagag   7800

cgtggagaaa gaggcagggg agaaggaaga tgaaaaggaa gcggttgtgc cgaaaaagaa   7860

gtcgtcaatt ccgttcattg aggatacaga aggggagacg gaagacaaga tcgatctgga   7920

ggaccctcga cagctcaagt tcattcctga ggcgtccaga gcgtgcactc tgtgtctgtc   7980

atacattagt gcgccggcat gtacgccatg tggacacttt ttctgttggg actgtatttc   8040

cgaatgggtg agagagaagc ccgagtgtcc cttgtgtcgg cagggtgtga gagagcagaa   8100

cttgttgcct atcagataat gacgaggtct ggc                                8133


<210>  75
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer PEX10-R-BsiWI

<400>  75
gatcaacgta cgcttcagca gtaactgtat tgctc                                35


<210>  76
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer PEX10-F1-SalI

<400>  76
gatcaagtcg acattgtaac tagtcctgga gggtc                                35


<210>  77
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer PEX10-F2-SalI

<400>  77
gatcaagtcg acgtcttagc gtcatgtatt ctcaag                               36


<210>  78
<211>  7277
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pEXP-MOD1

<400>  78
catggatcca ggcctgttaa cggccattac ggcctgcagg atccgaaaaa acctcccaca     60

cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc    120

agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt    180

ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgcgg    240

ccgcaagtgt ggatggggaa gtgagtgccc ggttctgtgt gcacaattgg caatccaaga    300

tggatggatt caacacaggg atatagcgag ctacgtggtg gtgcgaggat atagcaacgg    360

atatttatgt ttgacacttg agaatgtacg atacaagcac tgtccaagta caatactaaa    420

catactgtac atactcatac tcgtacccgg gcaacggttt cacttgagtg cagtggctag    480

tgctcttact cgtacagtgt gcaatactgc gtatcatagt ctttgatgta tatcgtattc    540

attcatgtta gttgcgtacg agccggaagc ataaagtgta aagcctgggg tgcctaatga    600

gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg    660

tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg    720

cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg    780

gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga    840

aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg    900

gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag    960

aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc   1020

gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg   1080

ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt   1140

cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc   1200

ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc   1260

actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg   1320

tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca   1380

gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc   1440

ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat   1500

cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt   1560

ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt   1620

tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc   1680

agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc   1740

gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata   1800

ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg   1860

gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc   1920

cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct   1980

acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa   2040

cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt   2100

cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca   2160

ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac   2220

tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca   2280

atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt   2340

tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc   2400

actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca   2460

aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata   2520

ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc   2580

ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc   2640

cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt   2700

acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc   2760

ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct   2820

ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat   2880

ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc   2940

acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc   3000

tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg   3060

atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttccattcgc   3120

cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc   3180

agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc   3240

agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat   3300

tgggtaccgg gccccccctc gaggtcgatg gtgtcgataa gcttgatatc gaattcatgt   3360

cacacaaacc gatcttcgcc tcaaggaaac ctaattctac atccgagaga ctgccgagat   3420

ccagtctaca ctgattaatt ttcgggccaa taatttaaaa aaatcgtgtt atataatatt   3480

atatgtatta tatatataca tcatgatgat actgacagtc atgtcccatt gctaaataga   3540

cagactccat ctgccgcctc caactgatgt tctcaatatt taaggggtca tctcgcattg   3600

tttaataata aacagactcc atctaccgcc tccaaatgat gttctcaaaa tatattgtat   3660

gaacttattt ttattactta gtattattag acaacttact tgctttatga aaaacacttc   3720

ctatttagga aacaatttat aatggcagtt cgttcattta acaatttatg tagaataaat   3780

gttataaatg cgtatgggaa atcttaaata tggatagcat aaatgatatc tgcattgcct   3840

aattcgaaat caacagcaac gaaaaaaatc ccttgtacaa cataaatagt catcgagaaa   3900

tatcaactat caaagaacag ctattcacac gttactattg agattattat tggacgagaa   3960

tcacacactc aactgtcttt ctctcttcta gaaatacagg tacaagtatg tactattctc   4020

attgttcata cttctagtca tttcatccca catattcctt ggatttctct ccaatgaatg   4080

acattctatc ttgcaaattc aacaattata ataagatata ccaaagtagc ggtatagtgg   4140

caatcaaaaa gcttctctgg tgtgcttctc gtatttattt ttattctaat gatccattaa   4200

aggtatatat ttatttcttg ttatataatc cttttgttta ttacatgggc tggatacata   4260

aaggtatttt gatttaattt tttgcttaaa ttcaatcccc cctcgttcag tgtcaactgt   4320

aatggtagga aattaccata cttttgaaga agcaaaaaaa atgaaagaaa aaaaaaatcg   4380

tatttccagg ttagacgttc cgcagaatct agaatgcggt atgcggtaca ttgttcttcg   4440

aacgtaaaag ttgcgctccc tgagatattg tacatttttg cttttacaag tacaagtaca   4500

tcgtacaact atgtactact gttgatgcat ccacaacagt ttgttttgtt tttttttgtt   4560

tttttttttt ctaatgattc attaccgcta tgtataccta cttgtacttg tagtaagccg   4620

ggttattggc gttcaattaa tcatagactt atgaatctgc acggtgtgcg ctgcgagtta   4680

cttttagctt atgcatgcta cttgggtgta atattgggat ctgttcggaa atcaacggat   4740

gctcaatcga tttcgacagt aattaattaa gtcatacaca agtcagcttt cttcgagcct   4800

catataagta taagtagttc aacgtattag cactgtaccc agcatctccg tatcgagaaa   4860

cacaacaaca tgccccattg gacagatcat gcggatacac aggttgtgca gtatcataca   4920

tactcgatca gacaggtcgt ctgaccatca tacaagctga acaagcgctc catacttgca   4980

cgctctctat atacacagtt aaattacata tccatagtct aacctctaac agttaatctt   5040

ctggtaagcc tcccagccag ccttctggta tcgcttggcc tcctcaatag gatctcggtt   5100

ctggccgtac agacctcggc cgacaattat gatatccgtt ccggtagaca tgacatcctc   5160

aacagttcgg tactgctgtc cgagagcgtc tcccttgtcg tcaagaccca ccccgggggt   5220

cagaataagc cagtcctcag agtcgccctt aggtcggttc tgggcaatga agccaaccac   5280

aaactcgggg tcggatcggg caagctcaat ggtctgcttg gagtactcgc cagtggccag   5340

agagcccttg caagacagct cggccagcat gagcagacct ctggccagct tctcgttggg   5400

agaggggact aggaactcct tgtactggga gttctcgtag tcagagacgt cctccttctt   5460

ctgttcagag acagtttcct cggcaccagc tcgcaggcca gcaatgattc cggttccggg   5520

tacaccgtgg gcgttggtga tatcggacca ctcggcgatt cggtgacacc ggtactggtg   5580

cttgacagtg ttgccaatat ctgcgaactt tctgtcctcg aacaggaaga aaccgtgctt   5640

aagagcaagt tccttgaggg ggagcacagt gccggcgtag gtgaagtcgt caatgatgtc   5700

gatatgggtt ttgatcatgc acacataagg tccgacctta tcggcaagct caatgagctc   5760

cttggtggtg gtaacatcca gagaagcaca caggttggtt ttcttggctg ccacgagctt   5820

gagcactcga gcggcaaagg cggacttgtg gacgttagct cgagcttcgt aggagggcat   5880

tttggtggtg aagaggagac tgaaataaat ttagtctgca gaacttttta tcggaacctt   5940

atctggggca gtgaagtata tgttatggta atagttacga gttagttgaa cttatagata   6000

gactggacta tacggctatc ggtccaaatt agaaagaacg tcaatggctc tctgggcgtc   6060

gcctttgccg acaaaaatgt gatcatgatg aaagccagca atgacgttgc agctgatatt   6120

gttgtcggcc aaccgcgccg aaaacgcagc tgtcagaccc acagcctcca acgaagaatg   6180

tatcgtcaaa gtgatccaag cacactcata gttggagtcg tactccaaag gcggcaatga   6240

cgagtcagac agatactcgt cgaccgtacg gggagtttgg cgcccgtttt ttcgagcccc   6300

acacgtttcg gtgagtatga gcggcggcag attcgagcgt ttccggtttc cgcggctgga   6360

cgagagccca tgatgggggc tcccaccacc agcaatcagg gccctgatta cacacccacc   6420

tgtaatgtca tgctgttcat cgatggttaa tgctgctgtg tgctgtgtgt gtgtgttgtt   6480

tggcgctcat tgttgcgtta tgcagcgtac accacaatat tggaagctta ttagcctttc   6540

tattttttcg tttgcaaggc ttaacaacat tgctgtggag agggatgggg atatggaggc   6600

cgctggaggg agtcggagag gcgttttgga gcggcttggc ctggcgccca gctcgcgaaa   6660

cgcacctagg accctttggc acgccgaaat gtgccacttt tcagtctagt aacgccttac   6720

ctacgtcatt ccatgcgtgc atgtttgcgc cttttttccc ttgcccttga tcgccacaca   6780

gtacagtgca ctgtacagtg gaggttttgg gggggtctta gatgggagct aaaagcggcc   6840

tagcggtaca ctagtgggat tgtatggagt ggcatggagc ctaggtggag cctgacagga   6900

cgcacgaccg gctagcccgt gacagacgat gggtggctcc tgttgtccac cgcgtacaaa   6960

tgtttgggcc aaagtcttgt cagccttgct tgcgaaccta attcccaatt ttgtcacttc   7020

gcacccccat tgatcgagcc ctaacccctg cccatcaggc aatccaatta agctcgcatt   7080

gtctgccttg tttagtttgg ctcctgcccg tttcggcgtc cacttgcaca aacacaaaca   7140

agcattatat ataaggctcg tctctccctc ccaaccacac tcactttttt gcccgtcttc   7200

ccttgctaac acaaaagtca agaacacaaa caaccacccc aaccccctta cacacaagac   7260

atatctacag caatggc                                                  7277


<210>  79
<211>  7559
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pPEX10-1

<400>  79
gtacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca     60

ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat    120

taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc    180

tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca    240

aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca    300

aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg    360

ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg    420

acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt    480

ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt    540

tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc    600

tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt    660

gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt    720

agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc    780

tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa    840

agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt    900

tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct    960

acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta   1020

tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa   1080

agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc   1140

tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact   1200

acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc   1260

tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt   1320

ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta   1380

agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg   1440

tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt   1500

acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc   1560

agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt   1620

actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc   1680

tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc   1740

gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa   1800

ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac   1860

tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa   1920

aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt   1980

tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa   2040

tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct   2100

gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc   2160

gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc   2220

acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt   2280

agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg   2340

ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt   2400

ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta   2460

taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt   2520

aacgcgaatt ttaacaaaat attaacgctt acaatttcca ttcgccattc aggctgcgca   2580

actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg   2640

gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta   2700

aaacgacggc cagtgaattg taatacgact cactataggg cgaattgggt accgggcccc   2760

ccctcgaggt cgatggtgtc gataagcttg atatcgaatt catgtcacac aaaccgatct   2820

tcgcctcaag gaaacctaat tctacatccg agagactgcc gagatccagt ctacactgat   2880

taattttcgg gccaataatt taaaaaaatc gtgttatata atattatatg tattatatat   2940

atacatcatg atgatactga cagtcatgtc ccattgctaa atagacagac tccatctgcc   3000

gcctccaact gatgttctca atatttaagg ggtcatctcg cattgtttaa taataaacag   3060

actccatcta ccgcctccaa atgatgttct caaaatatat tgtatgaact tatttttatt   3120

acttagtatt attagacaac ttacttgctt tatgaaaaac acttcctatt taggaaacaa   3180

tttataatgg cagttcgttc atttaacaat ttatgtagaa taaatgttat aaatgcgtat   3240

gggaaatctt aaatatggat agcataaatg atatctgcat tgcctaattc gaaatcaaca   3300

gcaacgaaaa aaatcccttg tacaacataa atagtcatcg agaaatatca actatcaaag   3360

aacagctatt cacacgttac tattgagatt attattggac gagaatcaca cactcaactg   3420

tctttctctc ttctagaaat acaggtacaa gtatgtacta ttctcattgt tcatacttct   3480

agtcatttca tcccacatat tccttggatt tctctccaat gaatgacatt ctatcttgca   3540

aattcaacaa ttataataag atataccaaa gtagcggtat agtggcaatc aaaaagcttc   3600

tctggtgtgc ttctcgtatt tatttttatt ctaatgatcc attaaaggta tatatttatt   3660

tcttgttata taatcctttt gtttattaca tgggctggat acataaaggt attttgattt   3720

aattttttgc ttaaattcaa tcccccctcg ttcagtgtca actgtaatgg taggaaatta   3780

ccatactttt gaagaagcaa aaaaaatgaa agaaaaaaaa aatcgtattt ccaggttaga   3840

cgttccgcag aatctagaat gcggtatgcg gtacattgtt cttcgaacgt aaaagttgcg   3900

ctccctgaga tattgtacat ttttgctttt acaagtacaa gtacatcgta caactatgta   3960

ctactgttga tgcatccaca acagtttgtt ttgttttttt ttgttttttt tttttctaat   4020

gattcattac cgctatgtat acctacttgt acttgtagta agccgggtta ttggcgttca   4080

attaatcata gacttatgaa tctgcacggt gtgcgctgcg agttactttt agcttatgca   4140

tgctacttgg gtgtaatatt gggatctgtt cggaaatcaa cggatgctca atcgatttcg   4200

acagtaatta attaagtcat acacaagtca gctttcttcg agcctcatat aagtataagt   4260

agttcaacgt attagcactg tacccagcat ctccgtatcg agaaacacaa caacatgccc   4320

cattggacag atcatgcgga tacacaggtt gtgcagtatc atacatactc gatcagacag   4380

gtcgtctgac catcatacaa gctgaacaag cgctccatac ttgcacgctc tctatataca   4440

cagttaaatt acatatccat agtctaacct ctaacagtta atcttctggt aagcctccca   4500

gccagccttc tggtatcgct tggcctcctc aataggatct cggttctggc cgtacagacc   4560

tcggccgaca attatgatat ccgttccggt agacatgaca tcctcaacag ttcggtactg   4620

ctgtccgaga gcgtctccct tgtcgtcaag acccaccccg ggggtcagaa taagccagtc   4680

ctcagagtcg cccttaggtc ggttctgggc aatgaagcca accacaaact cggggtcgga   4740

tcgggcaagc tcaatggtct gcttggagta ctcgccagtg gccagagagc ccttgcaaga   4800

cagctcggcc agcatgagca gacctctggc cagcttctcg ttgggagagg ggactaggaa   4860

ctccttgtac tgggagttct cgtagtcaga gacgtcctcc ttcttctgtt cagagacagt   4920

ttcctcggca ccagctcgca ggccagcaat gattccggtt ccgggtacac cgtgggcgtt   4980

ggtgatatcg gaccactcgg cgattcggtg acaccggtac tggtgcttga cagtgttgcc   5040

aatatctgcg aactttctgt cctcgaacag gaagaaaccg tgcttaagag caagttcctt   5100

gagggggagc acagtgccgg cgtaggtgaa gtcgtcaatg atgtcgatat gggttttgat   5160

catgcacaca taaggtccga ccttatcggc aagctcaatg agctccttgg tggtggtaac   5220

atccagagaa gcacacaggt tggttttctt ggctgccacg agcttgagca ctcgagcggc   5280

aaaggcggac ttgtggacgt tagctcgagc ttcgtaggag ggcattttgg tggtgaagag   5340

gagactgaaa taaatttagt ctgcagaact ttttatcgga accttatctg gggcagtgaa   5400

gtatatgtta tggtaatagt tacgagttag ttgaacttat agatagactg gactatacgg   5460

ctatcggtcc aaattagaaa gaacgtcaat ggctctctgg gcgtcgcctt tgccgacaaa   5520

aatgtgatca tgatgaaagc cagcaatgac gttgcagctg atattgttgt cggccaaccg   5580

cgccgaaaac gcagctgtca gacccacagc ctccaacgaa gaatgtatcg tcaaagtgat   5640

ccaagcacac tcatagttgg agtcgtactc caaaggcggc aatgacgagt cagacagata   5700

ctcgtcgaca ttgtaactag tcctggaggg tcttttttat ggataacctc catgtacgat   5760

gtatccaaga tctccacgta ctgtgttctg tttcctaagt aatacccaac aacctctcca   5820

acaaacactt gggaagatgc acttgtgctg agatgtcaag atgttagtac tgtactggat   5880

ggagagaata ttaataaata attgttaccc aactacatct tgtcgattga aagagatacc   5940

cctaagacag ataggatatc tgcaacccga ggaatgaacc ccccagcacc ggcacccttt   6000

ctattaacaa aatgccaact gaaatttgaa aagttcaact aaacttattt gacccacaaa   6060

aactcgtcaa aagtggcggc gaaagctggc aaatgatgac atccccttgg aactatgata   6120

tcccctcgga atcttcgtcc ccatttgcca catctacttg caacgccacg tctgcttact   6180

aagcaaccca aatctgcctc ggctcaaaat gtggggaagt tcacatgcat tcgctggtga   6240

atctgatctg acactacaac tacacaccag gtccaacatg agcgacaata cgacaatcaa   6300

aaagccgatc cgacccaaac cgatccggac ggaacgcctg ccttacgctg gggccgcaga   6360

aatcatccga gccaaccaga aagaccacta ctttgagtcc gtgcttgaac agcatctcgt   6420

cacgtttctg cagaaatgga agggagtacg atttatccac cagtacaagg aggagctgga   6480

gacggcgtcc aagtttgcat atctcggttt gtgtacgctt gtgggctcca agactctcgg   6540

agaagagtac accaatctca tgtacactat cagagaccga acagctctac cgggggtggt   6600

gagacggttt ggctacgtgc tttccaacac tctgtttcca tacctgtttg tgcgctacat   6660

gggcaagttg cgcgccaaac tgatgcgcga gtatccccat ctggtggagt acgacgaaga   6720

tgagcctgtg cccagcccgg aaacatggaa ggagcgggtc atcaagacgt ttgtgaacaa   6780

gtttgacaag ttcacggcgc tggaggggtt taccgcgatc cacttggcga ttttctacgt   6840

ctacggctcg tactaccagc tcagtaagcg gatctggggc atgcgttatg tatttggaca   6900

ccgactggac aagaatgagc ctcgaatcgg ttacgagatg ctcggtctgc tgattttcgc   6960

ccggtttgcc acgtcatttg tgcagacggg aagagagtac ctcggagcgc tgctggaaaa   7020

gagcgtggag aaagaggcag gggagaagga agatgaaaag gaagcggttg tgccgaaaaa   7080

gaagtcgtca attccgttca ttgaggatac agaaggggag acggaagaca agatcgatct   7140

ggaggaccct cgacagctca agttcattcc tgaggcgtcc agagcgtgca ctctgtgtct   7200

gtcatacatt agtgcgccgg catgtacgcc atgtggacac tttttctgtt gggactgtat   7260

ttccgaatgg gtgagagaga agcccgagtg tcccttgtgt cggcagggtg tgagagagca   7320

gaacttgttg cctatcagat aatgacgagg tctggatgga aggactagtc agcgagacac   7380

agagcatcag ggaccagaca cgaccaattc aatcgacaac actgtgctgc atagcagtgc   7440

acagaggtcc tgggcatgaa tatattttag cattggagat atgagtggta gagcgtatac   7500

agtattaatt gtggaggtat ctcgtcgcat tgatagagca atacagttac tgctgaagc    7559


<210>  80
<211>  8051
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pPEX10-2

<400>  80
gtacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca     60

ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat    120

taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc    180

tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca    240

aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca    300

aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg    360

ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg    420

acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt    480

ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt    540

tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc    600

tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt    660

gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt    720

agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc    780

tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa    840

agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt    900

tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct    960

acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta   1020

tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa   1080

agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc   1140

tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact   1200

acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc   1260

tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt   1320

ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta   1380

agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg   1440

tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt   1500

acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc   1560

agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt   1620

actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc   1680

tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc   1740

gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa   1800

ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac   1860

tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa   1920

aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt   1980

tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa   2040

tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct   2100

gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc   2160

gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc   2220

acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt   2280

agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg   2340

ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt   2400

ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta   2460

taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt   2520

aacgcgaatt ttaacaaaat attaacgctt acaatttcca ttcgccattc aggctgcgca   2580

actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg   2640

gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta   2700

aaacgacggc cagtgaattg taatacgact cactataggg cgaattgggt accgggcccc   2760

ccctcgaggt cgatggtgtc gataagcttg atatcgaatt catgtcacac aaaccgatct   2820

tcgcctcaag gaaacctaat tctacatccg agagactgcc gagatccagt ctacactgat   2880

taattttcgg gccaataatt taaaaaaatc gtgttatata atattatatg tattatatat   2940

atacatcatg atgatactga cagtcatgtc ccattgctaa atagacagac tccatctgcc   3000

gcctccaact gatgttctca atatttaagg ggtcatctcg cattgtttaa taataaacag   3060

actccatcta ccgcctccaa atgatgttct caaaatatat tgtatgaact tatttttatt   3120

acttagtatt attagacaac ttacttgctt tatgaaaaac acttcctatt taggaaacaa   3180

tttataatgg cagttcgttc atttaacaat ttatgtagaa taaatgttat aaatgcgtat   3240

gggaaatctt aaatatggat agcataaatg atatctgcat tgcctaattc gaaatcaaca   3300

gcaacgaaaa aaatcccttg tacaacataa atagtcatcg agaaatatca actatcaaag   3360

aacagctatt cacacgttac tattgagatt attattggac gagaatcaca cactcaactg   3420

tctttctctc ttctagaaat acaggtacaa gtatgtacta ttctcattgt tcatacttct   3480

agtcatttca tcccacatat tccttggatt tctctccaat gaatgacatt ctatcttgca   3540

aattcaacaa ttataataag atataccaaa gtagcggtat agtggcaatc aaaaagcttc   3600

tctggtgtgc ttctcgtatt tatttttatt ctaatgatcc attaaaggta tatatttatt   3660

tcttgttata taatcctttt gtttattaca tgggctggat acataaaggt attttgattt   3720

aattttttgc ttaaattcaa tcccccctcg ttcagtgtca actgtaatgg taggaaatta   3780

ccatactttt gaagaagcaa aaaaaatgaa agaaaaaaaa aatcgtattt ccaggttaga   3840

cgttccgcag aatctagaat gcggtatgcg gtacattgtt cttcgaacgt aaaagttgcg   3900

ctccctgaga tattgtacat ttttgctttt acaagtacaa gtacatcgta caactatgta   3960

ctactgttga tgcatccaca acagtttgtt ttgttttttt ttgttttttt tttttctaat   4020

gattcattac cgctatgtat acctacttgt acttgtagta agccgggtta ttggcgttca   4080

attaatcata gacttatgaa tctgcacggt gtgcgctgcg agttactttt agcttatgca   4140

tgctacttgg gtgtaatatt gggatctgtt cggaaatcaa cggatgctca atcgatttcg   4200

acagtaatta attaagtcat acacaagtca gctttcttcg agcctcatat aagtataagt   4260

agttcaacgt attagcactg tacccagcat ctccgtatcg agaaacacaa caacatgccc   4320

cattggacag atcatgcgga tacacaggtt gtgcagtatc atacatactc gatcagacag   4380

gtcgtctgac catcatacaa gctgaacaag cgctccatac ttgcacgctc tctatataca   4440

cagttaaatt acatatccat agtctaacct ctaacagtta atcttctggt aagcctccca   4500

gccagccttc tggtatcgct tggcctcctc aataggatct cggttctggc cgtacagacc   4560

tcggccgaca attatgatat ccgttccggt agacatgaca tcctcaacag ttcggtactg   4620

ctgtccgaga gcgtctccct tgtcgtcaag acccaccccg ggggtcagaa taagccagtc   4680

ctcagagtcg cccttaggtc ggttctgggc aatgaagcca accacaaact cggggtcgga   4740

tcgggcaagc tcaatggtct gcttggagta ctcgccagtg gccagagagc ccttgcaaga   4800

cagctcggcc agcatgagca gacctctggc cagcttctcg ttgggagagg ggactaggaa   4860

ctccttgtac tgggagttct cgtagtcaga gacgtcctcc ttcttctgtt cagagacagt   4920

ttcctcggca ccagctcgca ggccagcaat gattccggtt ccgggtacac cgtgggcgtt   4980

ggtgatatcg gaccactcgg cgattcggtg acaccggtac tggtgcttga cagtgttgcc   5040

aatatctgcg aactttctgt cctcgaacag gaagaaaccg tgcttaagag caagttcctt   5100

gagggggagc acagtgccgg cgtaggtgaa gtcgtcaatg atgtcgatat gggttttgat   5160

catgcacaca taaggtccga ccttatcggc aagctcaatg agctccttgg tggtggtaac   5220

atccagagaa gcacacaggt tggttttctt ggctgccacg agcttgagca ctcgagcggc   5280

aaaggcggac ttgtggacgt tagctcgagc ttcgtaggag ggcattttgg tggtgaagag   5340

gagactgaaa taaatttagt ctgcagaact ttttatcgga accttatctg gggcagtgaa   5400

gtatatgtta tggtaatagt tacgagttag ttgaacttat agatagactg gactatacgg   5460

ctatcggtcc aaattagaaa gaacgtcaat ggctctctgg gcgtcgcctt tgccgacaaa   5520

aatgtgatca tgatgaaagc cagcaatgac gttgcagctg atattgttgt cggccaaccg   5580

cgccgaaaac gcagctgtca gacccacagc ctccaacgaa gaatgtatcg tcaaagtgat   5640

ccaagcacac tcatagttgg agtcgtactc caaaggcggc aatgacgagt cagacagata   5700

ctcgtcgacg tcttagcgtc atgtattctc aagcttagtc agagagaagg actatggagg   5760

agaaggggag aattgagaag ggtatttgaa gggactttga aggtcgcgtg gaagaggtac   5820

ttgaagaggt atttgaaggt cacgtggaag aggtatttga agatcacgtg gaagaagtac   5880

ttgttttaca gagaatatcg gggtgatttt gacagtggga ttgtctccca agtcctaatc   5940

gtttgacatg ggagcagtga aaagtcgggc taaaaaaggg aatatcggaa atcggaaaga   6000

cggaaagaat tactggactc atgtttagta gatctgagca cttcaaattt gaaaatatct   6060

cttcaaacag cagatcggtt ggtcgtggag gtaccatcaa gggtaaaatc aaggctatca   6120

tcaagggcca tatatcgcaa gtttggggga agataatatg ttcatagtga atcagggttg   6180

tggatttcct catctaacgg cattgtaact agtcctggag ggtctttttt atggataacc   6240

tccatgtacg atgtatccaa gatctccacg tactgtgttc tgtttcctaa gtaataccca   6300

acaacctctc caacaaacac ttgggaagat gcacttgtgc tgagatgtca agatgttagt   6360

actgtactgg atggagagaa tattaataaa taattgttac ccaactacat cttgtcgatt   6420

gaaagagata cccctaagac agataggata tctgcaaccc gaggaatgaa ccccccagca   6480

ccggcaccct ttctattaac aaaatgccaa ctgaaatttg aaaagttcaa ctaaacttat   6540

ttgacccaca aaaactcgtc aaaagtggcg gcgaaagctg gcaaatgatg acatcccctt   6600

ggaactatga tatcccctcg gaatcttcgt ccccatttgc cacatctact tgcaacgcca   6660

cgtctgctta ctaagcaacc caaatctgcc tcggctcaaa atgtggggaa gttcacatgc   6720

attcgctggt gaatctgatc tgacactaca actacacacc aggtccaaca tgagcgacaa   6780

tacgacaatc aaaaagccga tccgacccaa accgatccgg acggaacgcc tgccttacgc   6840

tggggccgca gaaatcatcc gagccaacca gaaagaccac tactttgagt ccgtgcttga   6900

acagcatctc gtcacgtttc tgcagaaatg gaagggagta cgatttatcc accagtacaa   6960

ggaggagctg gagacggcgt ccaagtttgc atatctcggt ttgtgtacgc ttgtgggctc   7020

caagactctc ggagaagagt acaccaatct catgtacact atcagagacc gaacagctct   7080

accgggggtg gtgagacggt ttggctacgt gctttccaac actctgtttc catacctgtt   7140

tgtgcgctac atgggcaagt tgcgcgccaa actgatgcgc gagtatcccc atctggtgga   7200

gtacgacgaa gatgagcctg tgcccagccc ggaaacatgg aaggagcggg tcatcaagac   7260

gtttgtgaac aagtttgaca agttcacggc gctggagggg tttaccgcga tccacttggc   7320

gattttctac gtctacggct cgtactacca gctcagtaag cggatctggg gcatgcgtta   7380

tgtatttgga caccgactgg acaagaatga gcctcgaatc ggttacgaga tgctcggtct   7440

gctgattttc gcccggtttg ccacgtcatt tgtgcagacg ggaagagagt acctcggagc   7500

gctgctggaa aagagcgtgg agaaagaggc aggggagaag gaagatgaaa aggaagcggt   7560

tgtgccgaaa aagaagtcgt caattccgtt cattgaggat acagaagggg agacggaaga   7620

caagatcgat ctggaggacc ctcgacagct caagttcatt cctgaggcgt ccagagcgtg   7680

cactctgtgt ctgtcataca ttagtgcgcc ggcatgtacg ccatgtggac actttttctg   7740

ttgggactgt atttccgaat gggtgagaga gaagcccgag tgtcccttgt gtcggcaggg   7800

tgtgagagag cagaacttgt tgcctatcag ataatgacga ggtctggatg gaaggactag   7860

tcagcgagac acagagcatc agggaccaga cacgaccaat tcaatcgaca acactgtgct   7920

gcatagcagt gcacagaggt cctgggcatg aatatatttt agcattggag atatgagtgg   7980

tagagcgtat acagtattaa ttgtggaggt atctcgtcgc attgatagag caatacagtt   8040

actgctgaag c                                                        8051


<210>  81
<211>  15877
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZKL1-2SP98C

<400>  81
aaatgatgtc gacgcagtag gatgtcctgc acgggtcttt ttgtggggtg tggagaaagg     60

ggtgcttgga tcgatggaag ccggtagaac cgggctgctt gtgcttggag atggaagccg    120

gtagaaccgg gctgcttggg gggatttggg gccgctgggc tccaaagagg ggtaggcatt    180

tcgttggggt tacgtaattg cggcatttgg gtcctgcgcg catgtcccat tggtcagaat    240

tagtccggat aggagactta tcagccaatc acagcgccgg atccacctgt aggttgggtt    300

gggtgggagc acccctccac agagtagagt caaacagcag cagcaacatg atagttgggg    360

gtgtgcgtgt taaaggaaaa aaaagaagct tgggttatat tcccgctcta tttagaggtt    420

gcgggataga cgccgacgga gggcaatggc gctatggaac cttgcggata tccatacgcc    480

gcggcggact gcgtccgaac cagctccagc agcgtttttt ccgggccatt gagccgactg    540

cgaccccgcc aacgtgtctt ggcccacgca ctcatgtcat gttggtgttg ggaggccact    600

ttttaagtag cacaaggcac ctagctcgca gcaaggtgtc cgaaccaaag aagcggctgc    660

agtggtgcaa acggggcgga aacggcggga aaaagccacg ggggcacgaa ttgaggcacg    720

ccctcgaatt tgagacgagt cacggcccca ttcgcccgcg caatggctcg ccaacgcccg    780

gtcttttgca ccacatcagg ttaccccaag ccaaaccttt gtgttaaaaa gcttaacata    840

ttataccgaa cgtaggtttg ggcgggcttg ctccgtctgt ccaaggcaac atttatataa    900

gggtctgcat cgccggctca attgaatctt ttttcttctt ctcttctcta tattcattct    960

tgaattaaac acacatcaac catgggcgta ttcattaaac aggagcagct tccggctctc   1020

aagaagtaca agtactccgc cgaggatcac tcgttcatct ccaacaacat tctgcgcccc   1080

ttctggcgac agtttgtcaa aatcttccct ctgtggatgg cccccaacat ggtgactctg   1140

ctgggcttct tctttgtcat tgtgaacttc atcaccatgc tcattgttga tcccacccac   1200

gaccgcgagc ctcccagatg ggtctacctc acctacgctc tgggtctgtt cctttaccag   1260

acatttgatg cctgtgacgg atcccatgcc cgacgaactg gccagagtgg accccttgga   1320

gagctgtttg accactgtgt cgacgccatg aatacctctc tgattctcac ggtggtggtg   1380

tccaccaccc atatgggata taacatgaag ctactgattg tgcagattgc cgctctcgga   1440

aacttctacc tgtcgacctg ggagacctac cataccggaa ctctgtacct ttctggcttc   1500

tctggtcctg ttgaaggtat cttgattctg gtggctcttt tcgtcctcac cttcttcact   1560

ggtcccaacg tgtacgctct gaccgtctac gaggctcttc ccgagtccat cacttcgctg   1620

ctgcctgcca gcttcctgga cgtcaccatc acccagatct acattggatt cggagtgctg   1680

ggcatggtgt tcaacatcta cggcgcctgc ggaaacgtga tcaagtacta caacaacaag   1740

ggcaagagcg ctctccccgc cattctcgga atcgccccct ttggcatctt ctacgtcggc   1800

gtctttgcct gggcccatgt tgctcctctg cttctctcca agtacgccat cgtctatctg   1860

tttgccattg gggctgcctt tgccatgcaa gtcggccaga tgattcttgc ccatctcgtg   1920

cttgctccct ttccccactg gaacgtgctg ctcttcttcc cctttgtggg actggcagtg   1980

cactacattg cacccgtgtt tggctgggac gccgatatcg tgtcggttaa cactctcttc   2040

acctgttttg gcgccaccct ctccatttac gccttctttg tgcttgagat catcgacgag   2100

atcaccaact acctcgatat ctggtgtctg cgaatcaagt accctcagga gaagaagacc   2160

gaataagcgg ccgcatggag cgtgtgttct gagtcgatgt tttctatgga gttgtgagtg   2220

ttagtagaca tgatgggttt atatatgatg aatgaataga tgtgattttg atttgcacga   2280

tggaattgag aactttgtaa acgtacatgg gaatgtatga atgtgggggt tttgtgactg   2340

gataactgac ggtcagtgga cgccgttgtt caaatatcca agagatgcga gaaactttgg   2400

gtcaagtgaa catgtcctct ctgttcaagt aaaccatcaa ctatgggtag tatatttagt   2460

aaggacaaga gttgagattc tttggagtcc tagaaacgta ttttcgcgtt ccaagatcaa   2520

attagtagag taatacgggc acgggaatcc attcatagtc tcaatcctgc aggtgagtta   2580

attaatcgag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc   2640

tcacaattcc acacaacgta cgatagttag tagacaacaa tcagaacatc tccctcctta   2700

tataatcaca caggccagaa cgcgctaaac taaagcgctt tggacactat gttacattgg   2760

cattgattga actgaaacca cagtctccct cgcctgaatc gagcaatgga tgttgtcgga   2820

agtcaacttc actagaagag cggttctatg ccttgtcaag atcatatcat aaactcactc   2880

tgtattaccc catctataga acacttgtta tgaatgggcg gaaacattcc gctatatgca   2940

cctttccaca ctaatgcaaa gatgtgcatc ttcaacgggt agtaagactg gttccgactt   3000

ccgttgcatg gagagcaatg acctcgataa tgcgaacatc ccccacatat acactcttac   3060

acaggccaat ataatctgtg catttactaa atatttaagt ctatgcacct gcttgatgaa   3120

aagcggcacg gatggtatca tctagtttcc gccaatccaa gaaccaactg tgttggcagt   3180

ggtgtagccc atggcacaca gaccaaagat gaaaatacag acatcggcgg ttcgagccgt   3240

ggtgcctcga gcaacaccct tgtaatgcaa aagaggaggg taaatgtaca ccagaggcac   3300

acatgcaaac gatccggtga gagcgacgaa ccgatcgaga tcgtcggcac ctccccatgc   3360

aacaaaggcg gtgacaaaca caaggaagaa ccggaaaatg ttcttctgcc acttgatggt   3420

agagttgtac ttgcctgatc gggtgaagag accattctcg atgattcgga tggcgcgcca   3480

gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc   3540

cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc   3600

tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat   3660

gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt   3720

ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg   3780

aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc   3840

tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt   3900

ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa   3960

gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta   4020

tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa   4080

caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa   4140

ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt   4200

cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt   4260

ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat   4320

cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat   4380

gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc   4440

aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc   4500

acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta   4560

gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga   4620

cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg   4680

cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc   4740

tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat   4800

cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag   4860

gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat   4920

cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa   4980

ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa   5040

gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga   5100

taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg   5160

gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc   5220

acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg   5280

aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact   5340

cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat   5400

atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt   5460

gccacctgat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcagga   5520

aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt   5580

ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat   5640

agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa   5700

cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta   5760

atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc   5820

ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc   5880

gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac   5940

acccgccgcg cttaatgcgc cgctacaggg cgcgtccatt cgccattcag gctgcgcaac   6000

tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga   6060

tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa   6120

acgacggcca gtgaattgta atacgactca ctatagggcg aattgggccc gacgtcgcat   6180

gcttagaagt gaggattaca agaagcctct ggatatcaat gatgaacgta ctcagcggct   6240

ggtcaagcat ttcgaccgtc gaatcgacga ggtgttcacc tttgacaagc gagggttccc   6300

aattgatcac gttctcgagt tgttcaaatc ttctctcaac atctctctgc atgaactatc   6360

tctgttgacg aacgtgtcac ccactgttcc tcgaacgccc ttctccgagt ttggtctgaa   6420

catcttcgat ctcaaactga cccccgcagt gatcaatagt gccatgccac tgccgatgcg   6480

gtgcgaacat ccctggaggg attctcggag ctctacacaa tgcagattct gtcgtcgagt   6540

actctctacc ttgctcgaat gacttattgt gctactactg cactcatgct tcgatcatgt   6600

gccctactgc accccaaatt tggtgatctg attgagacag agtaccctct tcagctgatt   6660

cagaagatca tcagcaacat gaatgatgtg gttgaccagg caggctgttg tagtcacgtc   6720

cttcacttca agttcattct tcatctgctt ctgttttact ttgacaggca aatgaagaca   6780

tggtacgact tgatggaggc caagaacgcc atttcacccc gagacaccga agtgcctgaa   6840

atcctggctg cccccattga taacatcgga aactacggta ttccggaaag tgtatataga   6900

acctttcccc agcttgtgtc tgtggatatg gatggtgtaa tccccttaat taactcacct   6960

gcaggattga gactatgaat ggattcccgt gcccgtatta ctctactaat ttgatcttgg   7020

aacgcgaaaa tacgtttcta ggactccaaa gaatctcaac tcttgtcctt actaaatata   7080

ctacccatag ttgatggttt acttgaacag agaggacatg ttcacttgac ccaaagtttc   7140

tcgcatctct tggatatttg aacaacggcg tccactgacc gtcagttatc cagtcacaaa   7200

acccccacat tcatacattc ccatgtacgt ttacaaagtt ctcaattcca tcgtgcaaat   7260

caaaatcaca tctattcatt catcatatat aaacccatca tgtctactaa cactcacaac   7320

tccatagaaa acatcgactc agaacacacg ctccatgcgg ccgcttaggc aacgggcttg   7380

atgacagcgg gaggagtgcc cacattgttt cggtttcgaa agaacaggac acccttgcca   7440

gctccctcgg caccagcgga gggttcaacc cactggcaca ttcgtgcaga tcggtacatg   7500

gctcgaatga atcctcgagg accgtcctgg acatcagctc gatagtgctt gcccatgata   7560

ggtttgatgg cctcggtagc ttcgtccgca ttgtagaagg gaatggaaga gacgtagtga   7620

tgcaggacgt gagtctcgat aatgccgtgg agcagatgac gtccaatgaa gcccatctct   7680

cggtcgatgg ttgcagcggc acctcgcaca aagttccact cgtcgttggt gtagtgggga   7740

agagtaggat ctgtgtgctg cagaaaggta atggcgacga gccagtggtt aacccacaag   7800

tagggaacga agtaccagat ggccatgttg tagaatccga acttctgaac gagaaagtac   7860

agagcggtgg ccataagacc aatgccaatg tcggagagca cgatgagctt ggcgtcgctg   7920

ttctcgtaca gaggagatcg gggatcgaaa tggttaactc caccgccaag accgttgtgc   7980

tttcccttgc ctcgaccctc tcgctgccgc tcatggtagt tgtgtccagt aacgttggta   8040

atgagatagt tgggccaacc gaccagttgc tgaagcacaa gcatgagcag ggtgaaagca   8100

ggagtttcct cggtaagatg ggcgagttcg tgggtcatct tgccgagtcg agtagcttgc   8160

tgctctcggg ttcgaggaac gaagaccatg tctcgctcca tgtttccagt ggccttgtga   8220

tgcttccggt gggagatttg ccagctgaag tagggaacaa gcagggaaga gtgaagcacc   8280

cagccagtaa tgtcgttgat gattcgggaa tcggagaaag caccatgtcc acactcgtgg   8340

gcaatgaccc acagtccagt accgaagagt ccctgaagaa cggtgtacac agcccacaga   8400

ccggctcgag caggagtgga gggaatgtac tcgggtgtca caaagttgta ccagatgctg   8460

aaagtggtag tcaggaggac aatgtctcga agaatgtagc cgtatccctt gagagcagat   8520

cgcttgaagc agtgcttggg aatagcgttg tagatgtcct tgatggtgaa gtcgggaact   8580

tcgaactggt tgccgtaggt atccagcatg acaccgtact cggacttggg cttggcaatg   8640

tccacctcgg acatggaaga cagcgatgta gaggaggccg agtgtctggg agaatcggag   8700

ggagagacgg cagcagactc cgagtcggtc acagtggtgg aagtgacggt tcgtcggagg   8760

gcagggttct gcttgggcag agccgaggtg gaggccatgg ccattgctgt agatatgtct   8820

tgtgtgtaag ggggttgggg tggttgtttg tgttcttgac ttttgtgtta gcaagggaag   8880

acgggcaaaa aagtgagtgt ggttgggagg gagagacgag ccttatatat aatgcttgtt   8940

tgtgtttgtg caagtggacg ccgaaacggg caggagccaa actaaacaag gcagacaatg   9000

cgagcttaat tggattgcct gatgggcagg ggttagggct cgatcaatgg gggtgcgaag   9060

tgacaaaatt gggaattagg ttcgcaagca aggctgacaa gactttggcc caaacatttg   9120

tacgcggtgg acaacaggag ccacccatcg tctgtcacgg gctagccggt cgtgcgtcct   9180

gtcaggctcc acctaggctc catgccactc catacaatcc cactagtgta ccgctaggcc   9240

gcttttagct cccatctaag acccccccaa aacctccact gtacagtgca ctgtactgtg   9300

tggcgatcaa gggcaaggga aaaaaggcgc aaacatgcac gcatggaatg acgtaggtaa   9360

ggcgttacta gactgaaaag tggcacattt cggcgtgcca aagggtccta ggtgcgtttc   9420

gcgagctggg cgccaggcca agccgctcca aaacgcctct ccgactccct ccagcggcct   9480

ccatatcccc atccctctcc acagcaatgt tgttaagcct tgcaaacgaa aaaatagaaa   9540

ggctaataag cttccaatat tgtggtgtac gctgcataac gcaacaatga gcgccaaaca   9600

acacacacac acagcacaca gcagcattaa ccacgatgaa cagcatgaat tcctttacct   9660

gcaggataac ttcgtataat gtatgctata cgaagttatg atctctctct tgagcttttc   9720

cataacaagt tcttctgcct ccaggaagtc catgggtggt ttgatcatgg ttttggtgta   9780

gtggtagtgc agtggtggta ttgtgactgg ggatgtagtt gagaataagt catacacaag   9840

tcagctttct tcgagcctca tataagtata agtagttcaa cgtattagca ctgtacccag   9900

catctccgta tcgagaaaca caacaacatg ccccattgga cagatcatgc ggatacacag   9960

gttgtgcagt atcatacata ctcgatcaga caggtcgtct gaccatcata caagctgaac  10020

aagcgctcca tacttgcacg ctctctatat acacagttaa attacatatc catagtctaa  10080

cctctaacag ttaatcttct ggtaagcctc ccagccagcc ttctggtatc gcttggcctc  10140

ctcaatagga tctcggttct ggccgtacag acctcggccg acaattatga tatccgttcc  10200

ggtagacatg acatcctcaa cagttcggta ctgctgtccg agagcgtctc ccttgtcgtc  10260

aagacccacc ccgggggtca gaataagcca gtcctcagag tcgcccttag gtcggttctg  10320

ggcaatgaag ccaaccacaa actcggggtc ggatcgggca agctcaatgg tctgcttgga  10380

gtactcgcca gtggccagag agcccttgca agacagctcg gccagcatga gcagacctct  10440

ggccagcttc tcgttgggag aggggactag gaactccttg tactgggagt tctcgtagtc  10500

agagacgtcc tccttcttct gttcagagac agtttcctcg gcaccagctc gcaggccagc  10560

aatgattccg gttccgggta caccgtgggc gttggtgata tcggaccact cggcgattcg  10620

gtgacaccgg tactggtgct tgacagtgtt gccaatatct gcgaactttc tgtcctcgaa  10680

caggaagaaa ccgtgcttaa gagcaagttc cttgaggggg agcacagtgc cggcgtaggt  10740

gaagtcgtca atgatgtcga tatgggtttt gatcatgcac acataaggtc cgaccttatc  10800

ggcaagctca atgagctcct tggtggtggt aacatccaga gaagcacaca ggttggtttt  10860

cttggctgcc acgagcttga gcactcgagc ggcaaaggcg gacttgtgga cgttagctcg  10920

agcttcgtag gagggcattt tggtggtgaa gaggagactg aaataaattt agtctgcaga  10980

actttttatc ggaaccttat ctggggcagt gaagtatatg ttatggtaat agttacgagt  11040

tagttgaact tatagataga ctggactata cggctatcgg tccaaattag aaagaacgtc  11100

aatggctctc tgggcgtcgc ctttgccgac aaaaatgtga tcatgatgaa agccagcaat  11160

gacgttgcag ctgatattgt tgtcggccaa ccgcgccgaa aacgcagctg tcagacccac  11220

agcctccaac gaagaatgta tcgtcaaagt gatccaagca cactcatagt tggagtcgta  11280

ctccaaaggc ggcaatgacg agtcagacag atactcgtcg acgcgataac ttcgtataat  11340

gtatgctata cgaagttatc gtacgatagt tagtagacaa caatcgatcg aggaagagga  11400

caagcggctg cttcttaagt ttgtgacatc agtatccaag gcaccattgc aaggattcaa  11460

ggctttgaac ccgtcatttg ccattcgtaa cgctggtaga caggttgatc ggttccctac  11520

ggcctccacc tgtgtcaatc ttctcaagct gcctgactat caggacattg atcaacttcg  11580

gaagaaactt ttgtatgcca ttcgatcaca tgctggtttc gatttgtctt agaggaacgc  11640

atatacagta atcatagaga ataaacgata ttcatttatt aaagtagata gttgaggtag  11700

aagttgtaaa gagtgataaa tagcggccgc tcactgaatc tttttggctc ccttgtgctt  11760

tcggacgatg taggtctgca cgtagaagtt gaggaacaga cacaggacag taccaacgta  11820

gaagtagttg aaaaaccagc caaacattct cattccatct tgtcggtagc agggaatgtt  11880

ccggtacttc cagacgatgt agaagccaac gttgaactga atgatctgca tagaagtaat  11940

cagggacttg ggcataggga acttgagctt gatcagtcgg gtccaatagt agccgtacat  12000

gatccagtga atgaagccgt tgagcagcac aaagatccaa acggcttcgt ttcggtagtt  12060

gtagaacagc cacatgtcca taggagctcc gagatggtga aagaactgca accaggtcag  12120

aggcttgccc atgaggggca gatagaagga gtcaatgtac tcgaggaact tgctgaggta  12180

gaacagctga gtggtgattc ggaagacatt gttgtcgaaa gccttctcgc agttgtcgga  12240

catgacacca atggtgtaca tggcgtaggc catagagagg aaggagccca gcgagtagat  12300

ggacatgagc aggttgtagt tggtgaacac aaacttcatt cgagactgac ccttgggtcc  12360

gagaggacca agggtgaact tcaggatgac gaaggcgatg gagaggtaca gcacctcgca  12420

gtgcgaggca tcagaccaga gctgagcata gtcgaccttg ggaagaacct cctggccaat  12480

ggagacgatt tcgttcacga cctccatggt tgtgaattag ggtggtgaga atggttggtt  12540

gtagggaaga atcaaaggcc ggtctcggga tccgtgggta tatatatata tatatatata  12600

tacgatcctt cgttacctcc ctgttctcaa aactgtggtt tttcgttttt cgttttttgc  12660

tttttttgat ttttttaggg ccaactaagc ttccagattt cgctaatcac ctttgtacta  12720

attacaagaa aggaagaagc tgattagagt tgggcttttt atgcaactgt gctactcctt  12780

atctctgata tgaaagtgta gacccaatca catcatgtca tttagagttg gtaatactgg  12840

gaggatagat aaggcacgaa aacgagccat agcagacatg ctgggtgtag ccaagcagaa  12900

gaaagtagat gggagccaat tgacgagcga gggagctacg ccaatccgac atacgacacg  12960

ctgagatcgt cttggccggg gggtacctac agatgtccaa gggtaagtgc ttgactgtaa  13020

ttgtatgtct gaggacaaat atgtagtcag ccgtataaag tcataccagg caccagtgcc  13080

atcatcgaac cactaactct ctatgataca tgcctccggt attattgtac catgcgtcgc  13140

tttgttacat acgtatcttg cctttttctc tcagaaactc cagactttgg ctattggtcg  13200

agataagccc ggaccatagt gagtctttca cactctgttt aaacaccact aaaaccccac  13260

aaaatatatc ttaccgaata tacagatcta ctatagagga acaattgccc cggagaagac  13320

ggccaggccg cctagatgac aaattcaaca actcacagct gactttctgc cattgccact  13380

aggggggggc ctttttatat ggccaagcca agctctccac gtcggttggg ctgcacccaa  13440

caataaatgg gtagggttgc accaacaaag ggatgggatg gggggtagaa gatacgagga  13500

taacggggct caatggcaca aataagaacg aatactgcca ttaagactcg tgatccagcg  13560

actgacacca ttgcatcatc taagggcctc aaaactacct cggaactgct gcgctgatct  13620

ggacaccaca gaggttccga gcactttagg ttgcaccaaa tgtcccacca ggtgcaggca  13680

gaaaacgctg gaacagcgtg tacagtttgt cttaacaaaa agtgagggcg ctgaggtcga  13740

gcagggtggt gtgacttgtt atagccttta gagctgcgaa agcgcgtatg gatttggctc  13800

atcaggccag attgagggtc tgtggacaca tgtcatgtta gtgtacttca atcgccccct  13860

ggatatagcc ccgacaatag gccgtggcct catttttttg ccttccgcac atttccattg  13920

ctcggtaccc acaccttgct tctcctgcac ttgccaacct taatactggt ttacattgac  13980

caacatctta caagcggggg gcttgtctag ggtatatata aacagtggct ctcccaatcg  14040

gttgccagtc tcttttttcc tttctttccc cacagattcg aaatctaaac tacacatcac  14100

acaatgcctg ttactgacgt ccttaagcga aagtccggtg tcatcgtcgg cgacgatgtc  14160

cgagccgtga gtatccacga caagatcagt gtcgagacga cgcgttttgt gtaatgacac  14220

aatccgaaag tcgctagcaa cacacactct ctacacaaac taacccagct ctccatggtg  14280

aaggcttctc gacaggctct gcccctcgtc atcgacggaa aggtgtacga cgtctccgct  14340

tgggtgaact tccaccctgg tggagctgaa atcattgaga actaccaggg acgagatgct  14400

actgacgcct tcatggttat gcactctcag gaagccttcg acaagctcaa gcgaatgccc  14460

aagatcaacc aggcttccga gctgcctccc caggctgccg tcaacgaagc tcaggaggat  14520

ttccgaaagc tccgagaaga gctgatcgcc actggcatgt ttgacgcctc tcccctctgg  14580

tactcgtaca agatcttgac caccctgggt cttggcgtgc ttgccttctt catgctggtc  14640

cagtaccacc tgtacttcat tggtgctctc gtgctcggta tgcactacca gcaaatggga  14700

tggctgtctc atgacatctg ccaccaccag accttcaaga accgaaactg gaataacgtc  14760

ctgggtctgg tctttggcaa cggactccag ggcttctccg tgacctggtg gaaggacaga  14820

cacaacgccc atcattctgc taccaacgtt cagggtcacg atcccgacat tgataacctg  14880

cctctgctcg cctggtccga ggacgatgtc actcgagctt ctcccatctc ccgaaagctc  14940

attcagttcc aacagtacta tttcctggtc atctgtattc tcctgcgatt catctggtgt  15000

ttccagtctg tgctgaccgt tcgatccctc aaggaccgag acaaccagtt ctaccgatct  15060

cagtacaaga aagaggccat tggactcgct ctgcactgga ctctcaagac cctgttccac  15120

ctcttcttta tgccctccat cctgacctcg atgctggtgt tctttgtttc cgagctcgtc  15180

ggtggcttcg gaattgccat cgtggtcttc atgaaccact accctctgga gaagatcggt  15240

gattccgtct gggacggaca tggcttctct gtgggtcaga tccatgagac catgaacatt  15300

cgacgaggca tcattactga ctggttcttt ggaggcctga actaccagat cgagcaccat  15360

ctctggccca ccctgcctcg acacaacctc actgccgttt cctaccaggt ggaacagctg  15420

tgccagaagc acaacctccc ctaccgaaac cctctgcccc atgaaggtct cgtcatcctg  15480

ctccgatacc tgtcccagtt cgctcgaatg gccgagaagc agcccggtgc caaggctcag  15540

taagcggccg catgagaaga taaatatata aatacattga gatattaaat gcgctagatt  15600

agagagcctc atactgctcg gagagaagcc aagacgagta ctcaaagggg attacaccat  15660

ccatatccac agacacaagc tggggaaagg ttctatatac actttccgga ataccgtagt  15720

ttccgatgtt atcaatgggg gcagccagga tttcaggcac ttcggtgtct cggggtgaaa  15780

tggcgttctt ggcctccatc aagtcgtacc atgtcttcat ttgcctgtca aagtaaaaca  15840

gaagcagatg aagaatgaac ttgaagtgaa ggaattt                           15877


<210>  82
<211>  1185
<212>  DNA
<213>  Yarrowia lipolytica


<220>
<221>  CDS
<222>  (1)..(1185)
<223>  diacylglycerol cholinephosphotransferase (YlCPT1)

<300>
<302>  HIGH EICOSAPENTAENOIC ACID PRODUCING STRAINS OF
<310>  WO 2006/052870
<311>  2005-11-03
<312>  2006-05-18
<313>  (1)..(1185)

<300>
<302>  HIGH EICOSAPENTAENOIC ACID PRODUCING STRAINS OF
<310>  US 2006-0115881-A1
<311>  2005-11-02
<312>  2006-06-01
<313>  (1)..(1185)

<400>  82
atg ggc gta ttc att aaa cag gag cag ctt ccg gct ctc aag aag tac       48
Met Gly Val Phe Ile Lys Gln Glu Gln Leu Pro Ala Leu Lys Lys Tyr         
1               5                   10                  15              

aag tac tcc gcc gag gat cac tcg ttc atc tcc aac aac att ctg cgc       96
Lys Tyr Ser Ala Glu Asp His Ser Phe Ile Ser Asn Asn Ile Leu Arg         
            20                  25                  30                  

ccc ttc tgg cga cag ttt gtc aaa atc ttc cct ctg tgg atg gcc ccc      144
Pro Phe Trp Arg Gln Phe Val Lys Ile Phe Pro Leu Trp Met Ala Pro         
        35                  40                  45                      

aac atg gtg act ctg ttg ggc ttc ttc ttt gtc att gtg aac ttc atc      192
Asn Met Val Thr Leu Leu Gly Phe Phe Phe Val Ile Val Asn Phe Ile         
    50                  55                  60                          

acc atg ctc att gtt gat ccc acc cac gac cgc gag cct ccc aga tgg      240
Thr Met Leu Ile Val Asp Pro Thr His Asp Arg Glu Pro Pro Arg Trp         
65                  70                  75                  80          

gtc tac ctc acc tac gct ctg ggt ctg ttc ctt tac cag aca ttt gat      288
Val Tyr Leu Thr Tyr Ala Leu Gly Leu Phe Leu Tyr Gln Thr Phe Asp         
                85                  90                  95              

gcc tgt gac gga tcc cat gcc cga cga act ggc cag agt gga ccc ctt      336
Ala Cys Asp Gly Ser His Ala Arg Arg Thr Gly Gln Ser Gly Pro Leu         
            100                 105                 110                 

gga gag ctg ttt gac cac tgt gtc gac gcc atg aat acc tct ctg att      384
Gly Glu Leu Phe Asp His Cys Val Asp Ala Met Asn Thr Ser Leu Ile         
        115                 120                 125                     

ctc acg gtg gtg gtg tcc acc acc cat atg gga tat aac atg aag ctg      432
Leu Thr Val Val Val Ser Thr Thr His Met Gly Tyr Asn Met Lys Leu         
    130                 135                 140                         

ctg att gtg cag att gcc gct ctc gga aac ttc tac ctg tcg acc tgg      480
Leu Ile Val Gln Ile Ala Ala Leu Gly Asn Phe Tyr Leu Ser Thr Trp         
145                 150                 155                 160         

gag acc tac cat acc gga act ctg tac ctt tct ggc ttc tct ggt cct      528
Glu Thr Tyr His Thr Gly Thr Leu Tyr Leu Ser Gly Phe Ser Gly Pro         
                165                 170                 175             

gtt gaa ggt atc ttg att ctg gtg gct ctt ttc gtc ctc acc ttc ttc      576
Val Glu Gly Ile Leu Ile Leu Val Ala Leu Phe Val Leu Thr Phe Phe         
            180                 185                 190                 

act ggt ccc aac gtg tac gct ctg acc gtc tac gag gct ctt ccc gaa      624
Thr Gly Pro Asn Val Tyr Ala Leu Thr Val Tyr Glu Ala Leu Pro Glu         
        195                 200                 205                     

tcc atc act tcg ctg ctg cct gcc agc ttc ctg gac gtc acc atc acc      672
Ser Ile Thr Ser Leu Leu Pro Ala Ser Phe Leu Asp Val Thr Ile Thr         
    210                 215                 220                         

cag atc tac att gga ttc gga gtg ctg ggc atg gtg ttc aac atc tac      720
Gln Ile Tyr Ile Gly Phe Gly Val Leu Gly Met Val Phe Asn Ile Tyr         
225                 230                 235                 240         

ggc gcc tgc gga aac gtg atc aag tac tac aac aac aag ggc aag agc      768
Gly Ala Cys Gly Asn Val Ile Lys Tyr Tyr Asn Asn Lys Gly Lys Ser         
                245                 250                 255             

gct ctc ccc gcc att ctc gga atc gcc ccc ttt ggc atc ttc tac gtc      816
Ala Leu Pro Ala Ile Leu Gly Ile Ala Pro Phe Gly Ile Phe Tyr Val         
            260                 265                 270                 

ggc gtc ttt gcc tgg gcc cat gtt gct cct ctg ctt ctc tcc aag tac      864
Gly Val Phe Ala Trp Ala His Val Ala Pro Leu Leu Leu Ser Lys Tyr         
        275                 280                 285                     

gcc atc gtc tat ctg ttt gcc att ggg gct gcc ttt gcc atg caa gtc      912
Ala Ile Val Tyr Leu Phe Ala Ile Gly Ala Ala Phe Ala Met Gln Val         
    290                 295                 300                         

ggc cag atg att ctt gcc cat ctc gtg ctt gct ccc ttc ccc cac tgg      960
Gly Gln Met Ile Leu Ala His Leu Val Leu Ala Pro Phe Pro His Trp         
305                 310                 315                 320         

aac gtg ctg ctc ttc ttc ccc ttt gtg gga ctg gca gtg cac tac att     1008
Asn Val Leu Leu Phe Phe Pro Phe Val Gly Leu Ala Val His Tyr Ile         
                325                 330                 335             

gca ccc gtg ttt ggc tgg gac gcc gat atc gtg tcg gtt aac act ctc     1056
Ala Pro Val Phe Gly Trp Asp Ala Asp Ile Val Ser Val Asn Thr Leu         
            340                 345                 350                 

ttc acc tgt ttt ggc gcc acc ctc tcc att tac gcc ttc ttt gtg ctt     1104
Phe Thr Cys Phe Gly Ala Thr Leu Ser Ile Tyr Ala Phe Phe Val Leu         
        355                 360                 365                     

gag atc atc gac gag atc acc aac tac ctc gat atc tgg tgt ctg cga     1152
Glu Ile Ile Asp Glu Ile Thr Asn Tyr Leu Asp Ile Trp Cys Leu Arg         
    370                 375                 380                         

atc aag tac cct cag gag aag aag act gag taa                         1185
Ile Lys Tyr Pro Gln Glu Lys Lys Thr Glu                                 
385                 390                                                 


<210>  83
<211>  394
<212>  PRT
<213>  Yarrowia lipolytica

<400>  83

Met Gly Val Phe Ile Lys Gln Glu Gln Leu Pro Ala Leu Lys Lys Tyr 
1               5                   10                  15      


Lys Tyr Ser Ala Glu Asp His Ser Phe Ile Ser Asn Asn Ile Leu Arg 
            20                  25                  30          


Pro Phe Trp Arg Gln Phe Val Lys Ile Phe Pro Leu Trp Met Ala Pro 
        35                  40                  45              


Asn Met Val Thr Leu Leu Gly Phe Phe Phe Val Ile Val Asn Phe Ile 
    50                  55                  60                  


Thr Met Leu Ile Val Asp Pro Thr His Asp Arg Glu Pro Pro Arg Trp 
65                  70                  75                  80  


Val Tyr Leu Thr Tyr Ala Leu Gly Leu Phe Leu Tyr Gln Thr Phe Asp 
                85                  90                  95      


Ala Cys Asp Gly Ser His Ala Arg Arg Thr Gly Gln Ser Gly Pro Leu 
            100                 105                 110         


Gly Glu Leu Phe Asp His Cys Val Asp Ala Met Asn Thr Ser Leu Ile 
        115                 120                 125             


Leu Thr Val Val Val Ser Thr Thr His Met Gly Tyr Asn Met Lys Leu 
    130                 135                 140                 


Leu Ile Val Gln Ile Ala Ala Leu Gly Asn Phe Tyr Leu Ser Thr Trp 
145                 150                 155                 160 


Glu Thr Tyr His Thr Gly Thr Leu Tyr Leu Ser Gly Phe Ser Gly Pro 
                165                 170                 175     


Val Glu Gly Ile Leu Ile Leu Val Ala Leu Phe Val Leu Thr Phe Phe 
            180                 185                 190         


Thr Gly Pro Asn Val Tyr Ala Leu Thr Val Tyr Glu Ala Leu Pro Glu 
        195                 200                 205             


Ser Ile Thr Ser Leu Leu Pro Ala Ser Phe Leu Asp Val Thr Ile Thr 
    210                 215                 220                 


Gln Ile Tyr Ile Gly Phe Gly Val Leu Gly Met Val Phe Asn Ile Tyr 
225                 230                 235                 240 


Gly Ala Cys Gly Asn Val Ile Lys Tyr Tyr Asn Asn Lys Gly Lys Ser 
                245                 250                 255     


Ala Leu Pro Ala Ile Leu Gly Ile Ala Pro Phe Gly Ile Phe Tyr Val 
            260                 265                 270         


Gly Val Phe Ala Trp Ala His Val Ala Pro Leu Leu Leu Ser Lys Tyr 
        275                 280                 285             


Ala Ile Val Tyr Leu Phe Ala Ile Gly Ala Ala Phe Ala Met Gln Val 
    290                 295                 300                 


Gly Gln Met Ile Leu Ala His Leu Val Leu Ala Pro Phe Pro His Trp 
305                 310                 315                 320 


Asn Val Leu Leu Phe Phe Pro Phe Val Gly Leu Ala Val His Tyr Ile 
                325                 330                 335     


Ala Pro Val Phe Gly Trp Asp Ala Asp Ile Val Ser Val Asn Thr Leu 
            340                 345                 350         


Phe Thr Cys Phe Gly Ala Thr Leu Ser Ile Tyr Ala Phe Phe Val Leu 
        355                 360                 365             


Glu Ile Ile Asp Glu Ile Thr Asn Tyr Leu Asp Ile Trp Cys Leu Arg 
    370                 375                 380                 


Ile Lys Tyr Pro Gln Glu Lys Lys Thr Glu 
385                 390                 


<210>  84
<211>  15812
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZKL2-5U89GC

<400>  84
gtacgttatc atttgaacag tgaaaggcta cagtaacaga agcagttgta aacttcattc     60

cgttgattct gtactacagt accccactac gccgcttccg ctgacactgt tcaacccaaa    120

aactacatct gcgtgcgctg tgtaaggcta tcatcagata catactgtag attctgtaga    180

tgcgaacctg cttgtatcat atacatcccc ctccccctga cctgcacaag caagcaatgt    240

gacattgata ttgctgctta tctagtgccg aggatgtgaa agccgagact caaacatttc    300

ttttactctc ttgttcctga ccagacctgg cggagattac gccagtatga ttcttgcagg    360

tctgagacaa gcctggaaca gccaacattt atttttcgaa gcgagaaaca tgccacaccc    420

cggcacgttc agagatgcat atgatttgtt tttcgagtaa cagtaccccc cccccccccc    480

ccaatgaaac cagtattact cacaccatcc tcattcaaag cgttacactg attacgcgcc    540

catcaacgac agcatgaggg gactgctgat ctgatctaat caaatgacta caaaaatcgc    600

aataatgaag agcaaacgac aaaaaagaaa caggttaacc aatcccgctt caatgtctca    660

ccacaatcca gcactgtttc tcattacctc ctccctctaa tttcagagtt gcatcagggt    720

ccttgatggc gcgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc    780

gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc    840

ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata    900

acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg    960

cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct   1020

caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa   1080

gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc   1140

tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt   1200

aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg   1260

ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg   1320

cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct   1380

tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc   1440

tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg   1500

ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc   1560

aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt   1620

aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa   1680

aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat   1740

gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct   1800

gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg   1860

caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag   1920

ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta   1980

attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg   2040

ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg   2100

gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct   2160

ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta   2220

tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg   2280

gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc   2340

cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg   2400

gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga   2460

tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg   2520

ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat   2580

gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc   2640

tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca   2700

catttccccg aaaagtgcca cctgatgcgg tgtgaaatac cgcacagatg cgtaaggaga   2760

aaataccgca tcaggaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt   2820

gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa   2880

aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa   2940

agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac   3000

gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga   3060

accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa   3120

aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc   3180

tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tccattcgcc   3240

attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca   3300

gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca   3360

gtcacgacgt tgtaaaacga cggccagtga attgtaatac gactcactat agggcgaatt   3420

gggcccgacg tcgcatgctg gtttcgattt gtcttagagg aacgcatata cagtaatcat   3480

agagaataaa cgatattcat ttattaaagt agatagttga ggtagaagtt gtaaagagtg   3540

ataaatagct tagataccac agacaccctc ggtgacgaag tactgcagat ggtttccaat   3600

cacattgacc tgctggagca gagtgttacc ggcagagcac tgtttattgc tctggccctg   3660

gcacatgaca acgttggaga gaggagggtg gatcaggggc cagtcaataa agacctcacc   3720

agagcagtgc tggtaaccgt cccagaaggg cacttgaggg acgatatctc ctcggtgggt   3780

gattcggtag agctttcggt ctttggacac cttggagaca tcggggttct cctggccaaa   3840

gaagagttta tcgacccagt tagcaaagcc agcgttaccg acaatgggct gaccaagagt   3900

aacaacgagg ggatcgtggc cgttaacctt gaggttgatt ccgaacagaa gggctgcagc   3960

tcctccgaga gagtgaccgg tgacagcaat ctggtagtcg ggatactgct caatcacaga   4020

gtcgagcttg gggccgatct gattgtaggt gttgttgtag gactggatga agccattgtg   4080

gacaagacag tcatcacaag tagcagtaga agagatgtta gcagcaagat caaagttaat   4140

taactcacct gcaggattga gactatgaat ggattcccgt gcccgtatta ctctactaat   4200

ttgatcttgg aacgcgaaaa tacgtttcta ggactccaaa gaatctcaac tcttgtcctt   4260

actaaatata ctacccatag ttgatggttt acttgaacag agaggacatg ttcacttgac   4320

ccaaagtttc tcgcatctct tggatatttg aacaacggcg tccactgacc gtcagttatc   4380

cagtcacaaa acccccacat tcatacattc ccatgtacgt ttacaaagtt ctcaattcca   4440

tcgtgcaaat caaaatcaca tctattcatt catcatatat aaacccatca tgtctactaa   4500

cactcacaac tccatagaaa acatcgactc agaacacacg ctccatgcgg ccgcttagga   4560

atcctgagcg tccttgacac agtgaaccac accgactttg tgcatgtact tgagggtgga   4620

aatgatgttg cccacaatgg tagggtagaa gacgtaccga actccgtgtc gttcgcaaca   4680

ctctcggaca gcttgctgca cgaagggata gtgccaagac gacattcgag gaaagaggtg   4740

atgctcgatc tggaagttga gaccgccagt aaagaacatg gcaatgggtc caccgtaggt   4800

ggaagaggtc tccacctgag ctctgtacca gtcgatctga tcggcttcaa cgtccttctc   4860

ggagctcttg accttgcagt tcttgtcggg gattcgctcc gagccatcga agttgtgaga   4920

caagatgaaa aagaaggtga ggaaggcacc ggtagcagtg ggcaccagag gaatggtgat   4980

gagcagggag gttccagtga gataccaggg caagaaggcg gttcgaaaga tgaagaaagc   5040

tcgcataacg aatgcaaggg ttcggtaccg tcgcagaaag ccgttctctc gcatggctgt   5100

gacagactcg ggaatggtgt cgttgtgctg cattcggaag atgtagagag ggttgtacac   5160

cagcgaaacg ccgtaggctc caagcacgag gtacatgtac caggcctgga atcggtgaaa   5220

ccactttcga gcagtgttgg cagcagggta gttgtggaac acaaggaatg gttctgcgga   5280

ctcggcatcc aggtcgagac catgctgatt ggtgtaggtg tgatgtcgca tgatgtgaga   5340

ctgcagccag atccatctgg acgatccaat gacgtcgatg ccgtaggcaa agagagcgtt   5400

gacccagggc tttttgctga tggcaccatg agaggcatcg tgctgaatgg acaggccgat   5460

ctgcatgtgc atgaatccag tcaagagacc ccacagcacc attccggtag tagcccagtg   5520

ccactcgcaa aaggcggtga cagcaatgat gccaacggtt cgcagccaga atccaggtgt   5580

ggcataccag ttccgacctt tcatgacctc tcgcatagtt cgcttgacgt cctgtgcaaa   5640

gggagagtcg taggtgtaga caatgtcctt ggaggttcgg tcgtgcttgc ctcgcacgaa   5700

ctgttgaagc agcttcgagt tctcgggctt gacgtaaggg tgcatggagt agaacagagg   5760

agaagcatcg gaggcaccag aagcgaggat caagtcgcct ccgggatgga ccttggcaag   5820

accttccaga tcgtagagaa tgccgtcgat ggcaaccagg tcgggtcgct cgagcagctg   5880

ctcggtagta agggagagag ccatggttgt gaattagggt ggtgagaatg gttggttgta   5940

gggaagaatc aaaggccggt ctcgggatcc gtgggtatat atatatatat atatatatac   6000

gatccttcgt tacctccctg ttctcaaaac tgtggttttt cgtttttcgt tttttgcttt   6060

ttttgatttt tttagggcca actaagcttc cagatttcgc taatcacctt tgtactaatt   6120

acaagaaagg aagaagctga ttagagttgg gctttttatg caactgtgct actccttatc   6180

tctgatatga aagtgtagac ccaatcacat catgtcattt agagttggta atactgggag   6240

gatagataag gcacgaaaac gagccatagc agacatgctg ggtgtagcca agcagaagaa   6300

agtagatggg agccaattga cgagcgaggg agctacgcca atccgacata cgacacgctg   6360

agatcgtctt ggccgggggg tacctacaga tgtccaaggg taagtgcttg actgtaattg   6420

tatgtctgag gacaaatatg tagtcagccg tataaagtca taccaggcac cagtgccatc   6480

atcgaaccac taactctcta tgatacatgc ctccggtatt attgtaccat gcgtcgcttt   6540

gttacatacg tatcttgcct ttttctctca gaaactccag aattctctct cttgagcttt   6600

tccataacaa gttcttctgc ctccaggaag tccatgggtg gtttgatcat ggttttggtg   6660

tagtggtagt gcagtggtgg tattgtgact ggggatgtag ttgagaataa gtcatacaca   6720

agtcagcttt cttcgagcct catataagta taagtagttc aacgtattag cactgtaccc   6780

agcatctccg tatcgagaaa cacaacaaca tgccccattg gacagatcat gcggatacac   6840

aggttgtgca gtatcataca tactcgatca gacaggtcgt ctgaccatca tacaagctga   6900

acaagcgctc catacttgca cgctctctat atacacagtt aaattacata tccatagtct   6960

aacctctaac agttaatctt ctggtaagcc tcccagccag ccttctggta tcgcttggcc   7020

tcctcaatag gatctcggtt ctggccgtac agacctcggc cgacaattat gatatccgtt   7080

ccggtagaca tgacatcctc aacagttcgg tactgctgtc cgagagcgtc tcccttgtcg   7140

tcaagaccca ccccgggggt cagaataagc cagtcctcag agtcgccctt aggtcggttc   7200

tgggcaatga agccaaccac aaactcgggg tcggatcggg caagctcaat ggtctgcttg   7260

gagtactcgc cagtggccag agagcccttg caagacagct cggccagcat gagcagacct   7320

ctggccagct tctcgttggg agaggggact aggaactcct tgtactggga gttctcgtag   7380

tcagagacgt cctccttctt ctgttcagag acagtttcct cggcaccagc tcgcaggcca   7440

gcaatgattc cggttccggg tacaccgtgg gcgttggtga tatcggacca ctcggcgatt   7500

cggtgacacc ggtactggtg cttgacagtg ttgccaatat ctgcgaactt tctgtcctcg   7560

aacaggaaga aaccgtgctt aagagcaagt tccttgaggg ggagcacagt gccggcgtag   7620

gtgaagtcgt caatgatgtc gatatgggtt ttgatcatgc acacataagg tccgacctta   7680

tcggcaagct caatgagctc cttggtggtg gtaacatcca gagaagcaca caggttggtt   7740

ttcttggctg ccacgagctt gagcactcga gcggcaaagg cggacttgtg gacgttagct   7800

cgagcttcgt aggagggcat tttggtggtg aagaggagac tgaaataaat ttagtctgca   7860

gaacttttta tcggaacctt atctggggca gtgaagtata tgttatggta atagttacga   7920

gttagttgaa cttatagata gactggacta tacggctatc ggtccaaatt agaaagaacg   7980

tcaatggctc tctgggcgtc gcctttgccg acaaaaatgt gatcatgatg aaagccagca   8040

atgacgttgc agctgatatt gttgtcggcc aaccgcgccg aaaacgcagc tgtcagaccc   8100

acagcctcca acgaagaatg tatcgtcaaa gtgatccaag cacactcata gttggagtcg   8160

tactccaaag gcggcaatga cgagtcagac agatactcgt cgaccttttc cttgggaacc   8220

accaccgtca gcccttctga ctcacgtatt gtagccaccg acacaggcaa cagtccgtgg   8280

atagcagaat atgtcttgtc ggtccatttc tcaccaactt taggcgtcaa gtgaatgttg   8340

cagaagaagt atgtgccttc attgagaatc ggtgttgctg atttcaataa agtcttgaga   8400

tcagtttggc cagtcatgtt gtggggggta attggattga gttatcgcct acagtctgta   8460

caggtatact cgctgcccac tttatacttt ttgattccgc tgcacttgaa gcaatgtcgt   8520

ttaccaaaag tgagaatgct ccacagaaca caccccaggg tatggttgag caaaaaataa   8580

acactccgat acggggaatc gaaccccggt ctccacggtt ctcaagaagt attcttgatg   8640

agagcgtatc gatcgaggaa gaggacaagc ggctgcttct taagtttgtg acatcagtat   8700

ccaaggcacc attgcaagga ttcaaggctt tgaacccgtc atttgccatt cgtaacgctg   8760

gtagacaggt tgatcggttc cctacggcct ccacctgtgt caatcttctc aagctgcctg   8820

actatcagga cattgatcaa cttcggaaga aacttttgta tgccattcga tcacatgctg   8880

gtttcgattt gtcttagagg aacgcatata cagtaatcat agagaataaa cgatattcat   8940

ttattaaagt agatagttga ggtagaagtt gtaaagagtg ataaatagcg gccgctcact   9000

gaatcttttt ggctcccttg tgctttcgga cgatgtaggt ctgcacgtag aagttgagga   9060

acagacacag gacagtacca acgtagaagt agttgaaaaa ccagccaaac attctcattc   9120

catcttgtcg gtagcaggga atgttccggt acttccagac gatgtagaag ccaacgttga   9180

actgaatgat ctgcatagaa gtaatcaggg acttgggcat agggaacttg agcttgatca   9240

gtcgggtcca atagtagccg tacatgatcc agtgaatgaa gccgttgagc agcacaaaga   9300

tccaaacggc ttcgtttcgg tagttgtaga acagccacat gtccatagga gctccgagat   9360

ggtgaaagaa ctgcaaccag gtcagaggct tgcccatgag gggcagatag aaggagtcaa   9420

tgtactcgag gaacttgctg aggtagaaca gctgagtggt gattcggaag acattgttgt   9480

cgaaagcctt ctcgcagttg tcggacatga caccaatggt gtacatggcg taggccatag   9540

agaggaagga gcccagcgag tagatggaca tgagcaggtt gtagttggtg aacacaaact   9600

tcattcgaga ctgacccttg ggtccgagag gaccaagggt gaacttcagg atgacgaagg   9660

cgatggagag gtacagcacc tcgcagtgcg aggcatcaga ccagagctga gcatagtcga   9720

ccttgggaag aacctcctgg ccaatggaga cgatttcgtt cacgacctcc atggttgatg   9780

tgtgtttaat tcaagaatga atatagagaa gagaagaaga aaaaagattc aattgagccg   9840

gcgatgcaga cccttatata aatgttgcct tggacagacg gagcaagccc gcccaaacct   9900

acgttcggta taatatgtta agctttttaa cacaaaggtt tggcttgggg taacctgatg   9960

tggtgcaaaa gaccgggcgt tggcgagcca ttgcgcgggc gaatggggcc gtgactcgtc  10020

tcaaattcga gggcgtgcct caattcgtgc ccccgtggct ttttcccgcc gtttccgccc  10080

cgtttgcacc actgcagccg cttctttggt tcggacacct tgctgcgagc taggtgcctt  10140

gtgctactta aaaagtggcc tcccaacacc aacatgacat gagtgcgtgg gccaagacac  10200

gttggcgggg tcgcagtcgg ctcaatggcc cggaaaaaac gctgctggag ctggttcgga  10260

cgcagtccgc cgcggcgtat ggatatccgc aaggttccat agcgccattg ccctccgtcg  10320

gcgtctatcc cgcaacctct aaatagagcg ggaatataac ccaagcttct tttttttcct  10380

ttaacacgca cacccccaac tatcatgttg ctgctgctgt ttgactctac tctgtggagg  10440

ggtgctccca cccaacccaa cctacaggtg gatccggcgc tgtgattggc tgataagtct  10500

cctatccgga ctaattctga ccaatgggac atgcgcgcag gacccaaatg ccgcaattac  10560

gtaaccccaa cgaaatgcct acccctcttt ggagcccagc ggccccaaat ccccccaagc  10620

agcccggttc taccggcttc catctccaag cacaagcagc ccggttctac cggcttccat  10680

ctccaagcac ccctttctcc acaccccaca aaaagacccg tgcaggacat cctactgcgt  10740

gtttaaacac cactaaaacc ccacaaaata tatcttaccg aatatacaga tctactatag  10800

aggaacaatt gccccggaga agacggccag gccgcctaga tgacaaattc aacaactcac  10860

agctgacttt ctgccattgc cactaggggg gggccttttt atatggccaa gccaagctct  10920

ccacgtcggt tgggctgcac ccaacaataa atgggtaggg ttgcaccaac aaagggatgg  10980

gatggggggt agaagatacg aggataacgg ggctcaatgg cacaaataag aacgaatact  11040

gccattaaga ctcgtgatcc agcgactgac accattgcat catctaaggg cctcaaaact  11100

acctcggaac tgctgcgctg atctggacac cacagaggtt ccgagcactt taggttgcac  11160

caaatgtccc accaggtgca ggcagaaaac gctggaacag cgtgtacagt ttgtcttaac  11220

aaaaagtgag ggcgctgagg tcgagcaggg tggtgtgact tgttatagcc tttagagctg  11280

cgaaagcgcg tatggatttg gctcatcagg ccagattgag ggtctgtgga cacatgtcat  11340

gttagtgtac ttcaatcgcc ccctggatat agccccgaca ataggccgtg gcctcatttt  11400

tttgccttcc gcacatttcc attgctcggt acccacacct tgcttctcct gcacttgcca  11460

accttaatac tggtttacat tgaccaacat cttacaagcg gggggcttgt ctagggtata  11520

tataaacagt ggctctccca atcggttgcc agtctctttt ttcctttctt tccccacaga  11580

ttcgaaatct aaactacaca tcacacaatg cctgttactg acgtccttaa gcgaaagtcc  11640

ggtgtcatcg tcggcgacga tgtccgagcc gtgagtatcc acgacaagat cagtgtcgag  11700

acgacgcgtt ttgtgtaatg acacaatccg aaagtcgcta gcaacacaca ctctctacac  11760

aaactaaccc agctctccat ggtgaaggct tctcgacagg ctctgcccct cgtcatcgac  11820

ggaaaggtgt acgacgtctc cgcttgggtg aacttccacc ctggtggagc tgaaatcatt  11880

gagaactacc agggacgaga tgctactgac gccttcatgg ttatgcactc tcaggaagcc  11940

ttcgacaagc tcaagcgaat gcccaagatc aaccaggctt ccgagctgcc tccccaggct  12000

gccgtcaacg aagctcagga ggatttccga aagctccgag aagagctgat cgccactggc  12060

atgtttgacg cctctcccct ctggtactcg tacaagatct tgaccaccct gggtcttggc  12120

gtgcttgcct tcttcatgct ggtccagtac cacctgtact tcattggtgc tctcgtgctc  12180

ggtatgcact accagcaaat gggatggctg tctcatgaca tctgccacca ccagaccttc  12240

aagaaccgaa actggaataa cgtcctgggt ctggtctttg gcaacggact ccagggcttc  12300

tccgtgacct ggtggaagga cagacacaac gcccatcatt ctgctaccaa cgttcagggt  12360

cacgatcccg acattgataa cctgcctctg ctcgcctggt ccgaggacga tgtcactcga  12420

gcttctccca tctcccgaaa gctcattcag ttccaacagt actatttcct ggtcatctgt  12480

attctcctgc gattcatctg gtgtttccag tctgtgctga ccgttcgatc cctcaaggac  12540

cgagacaacc agttctaccg atctcagtac aagaaagagg ccattggact cgctctgcac  12600

tggactctca agaccctgtt ccacctcttc tttatgccct ccatcctgac ctcgatgctg  12660

gtgttctttg tttccgagct cgtcggtggc ttcggaattg ccatcgtggt cttcatgaac  12720

cactaccctc tggagaagat cggtgattcc gtctgggacg gacatggctt ctctgtgggt  12780

cagatccatg agaccatgaa cattcgacga ggcatcatta ctgactggtt ctttggaggc  12840

ctgaactacc agatcgagca ccatctctgg cccaccctgc ctcgacacaa cctcactgcc  12900

gtttcctacc aggtggaaca gctgtgccag aagcacaacc tcccctaccg aaaccctctg  12960

ccccatgaag gtctcgtcat cctgctccga tacctgtccc agttcgctcg aatggccgag  13020

aagcagcccg gtgccaaggc tcagtaagcg gccgcatgag aagataaata tataaataca  13080

ttgagatatt aaatgcgcta gattagagag cctcatactg ctcggagaga agccaagacg  13140

agtactcaaa ggggattaca ccatccatat ccacagacac aagctgggga aaggttctat  13200

atacactttc cggaataccg tagtttccga tgttatcaat gggggcagcc aggatttcag  13260

gcacttcggt gtctcggggt gaaatggcgt tcttggcctc catcaagtcg taccatgtct  13320

tcatttgcct gtcaaagtaa aacagaagca gatgaagaat gaacttgaag tgaaggaatt  13380

taaatagttg gagcaaggga gaaatgtaga gtgtgaaaga ctcactatgg tccgggctta  13440

tctcgaccaa tagccaaagt ctggagtttc tgagagaaaa aggcaagata cgtatgtaac  13500

aaagcgacgc atggtacaat aataccggag gcatgtatca tagagagtta gtggttcgat  13560

gatggcactg gtgcctggta tgactttata cggctgacta catatttgtc ctcagacata  13620

caattacagt caagcactta cccttggaca tctgtaggta ccccccggcc aagacgatct  13680

cagcgtgtcg tatgtcggat tggcgtagct ccctcgctcg tcaattggct cccatctact  13740

ttcttctgct tggctacacc cagcatgtct gctatggctc gttttcgtgc cttatctatc  13800

ctcccagtat taccaactct aaatgacatg atgtgattgg gtctacactt tcatatcaga  13860

gataaggagt agcacagttg cataaaaagc ccaactctaa tcagcttctt cctttcttgt  13920

aattagtaca aaggtgatta gcgaaatctg gaagcttagt tggccctaaa aaaatcaaaa  13980

aaagcaaaaa acgaaaaacg aaaaaccaca gttttgagaa cagggaggta acgaaggatc  14040

gtatatatat atatatatat atatacccac ggatcccgag accggccttt gattcttccc  14100

tacaaccaac cattctcacc accctaattc acaaccatgg gcgtattcat taaacaggag  14160

cagcttccgg ctctcaagaa gtacaagtac tccgccgagg atcactcgtt catctccaac  14220

aacattctgc gccccttctg gcgacagttt gtcaaaatct tccctctgtg gatggccccc  14280

aacatggtga ctctgctggg cttcttcttt gtcattgtga acttcatcac catgctcatt  14340

gttgatccca cccacgaccg cgagcctccc agatgggtct acctcaccta cgctctgggt  14400

ctgttccttt accagacatt tgatgcctgt gacggatccc atgcccgacg aactggccag  14460

agtggacccc ttggagagct gtttgaccac tgtgtcgacg ccatgaatac ctctctgatt  14520

ctcacggtgg tggtgtccac cacccatatg ggatataaca tgaagctact gattgtgcag  14580

attgccgctc tcggaaactt ctacctgtcg acctgggaga cctaccatac cggaactctg  14640

tacctttctg gcttctctgg tcctgttgaa ggtatcttga ttctggtggc tcttttcgtc  14700

ctcaccttct tcactggtcc caacgtgtac gctctgaccg tctacgaggc tcttcccgag  14760

tccatcactt cgctgctgcc tgccagcttc ctggacgtca ccatcaccca gatctacatt  14820

ggattcggag tgctgggcat ggtgttcaac atctacggcg cctgcggaaa cgtgatcaag  14880

tactacaaca acaagggcaa gagcgctctc cccgccattc tcggaatcgc cccctttggc  14940

atcttctacg tcggcgtctt tgcctgggcc catgttgctc ctctgcttct ctccaagtac  15000

gccatcgtct atctgtttgc cattggggct gcctttgcca tgcaagtcgg ccagatgatt  15060

cttgcccatc tcgtgcttgc tccctttccc cactggaacg tgctgctctt cttccccttt  15120

gtgggactgg cagtgcacta cattgcaccc gtgtttggct gggacgccga tatcgtgtcg  15180

gttaacactc tcttcacctg ttttggcgcc accctctcca tttacgcctt ctttgtgctt  15240

gagatcatcg acgagatcac caactacctc gatatctggt gtctgcgaat caagtaccct  15300

caggagaaga agaccgaata agcggccgca tggagcgtgt gttctgagtc gatgttttct  15360

atggagttgt gagtgttagt agacatgatg ggtttatata tgatgaatga atagatgtga  15420

ttttgatttg cacgatggaa ttgagaactt tgtaaacgta catgggaatg tatgaatgtg  15480

ggggttttgt gactggataa ctgacggtca gtggacgccg ttgttcaaat atccaagaga  15540

tgcgagaaac tttgggtcaa gtgaacatgt cctctctgtt caagtaaacc atcaactatg  15600

ggtagtatat ttagtaagga caagagttga gattctttgg agtcctagaa acgtattttc  15660

gcgttccaag atcaaattag tagagtaata cgggcacggg aatccattca tagtctcaat  15720

cctgcaggtg agttaattaa tcgagcttgg cgtaatcatg gtcatagctg tttcctgtgt  15780

gaaattgtta tccgctcaca attccacaca ac                                15812


<210>  85
<211>  4313
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZKUM

<400>  85
taatcgagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc     60

acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga    120

gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg    180

tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg    240

cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg    300

gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga    360

aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg    420

gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag    480

aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc    540

gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg    600

ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt    660

cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc    720

ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc    780

actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg    840

tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca    900

gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc    960

ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat   1020

cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt   1080

ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt   1140

tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc   1200

agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc   1260

gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata   1320

ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg   1380

gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc   1440

cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct   1500

acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa   1560

cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt   1620

cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca   1680

ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac   1740

tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca   1800

atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt   1860

tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc   1920

actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca   1980

aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata   2040

ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc   2100

ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc   2160

cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt   2220

acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc   2280

ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct   2340

ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat   2400

ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc   2460

acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc   2520

tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg   2580

atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttccattcgc   2640

cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc   2700

agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc   2760

agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat   2820

tgggtaccgg gccccccctc gaggtcgacg agtatctgtc tgactcgtca ttgccgcctt   2880

tggagtacga ctccaactat gagtgtgctt ggatcacttt gacgatacat tcttcgttgg   2940

aggctgtggg tctgacagct gcgttttcgg cgcggttggc cgacaacaat atcagctgca   3000

acgtcattgc tggctttcat catgatcaca tttttgtcgg caaaggcgac gcccagagag   3060

ccattgacgt tctttctaat ttggaccgat agccgtatag tccagtctat ctataagttc   3120

aactaactcg taactattac cataacatat acttcactgc cccagataag gttccgataa   3180

aaagttctgc agactaaatt tatttcagtc tcctcttcac caccaaaatg ccctcctacg   3240

aagctcgagt gctcaagctc gtggcagcca agaaaaccaa cctgtgtgct tctctggatg   3300

ttaccaccac caaggagctc attgagcttg ccgataaggt cggaccttat gtgtgcatga   3360

tcaaaaccca tatcgacatc attgacgact tcacctacgc cggcactgtg ctccccctca   3420

aggaacttgc tcttaagcac ggtttcttcc tgttcgagga cagaaagttc gcagatattg   3480

gcaacactgt caagcaccag taccggtgtc accgaatcgc cgagtggtcc gatatcacca   3540

acgcccacgg tgtacccgga accggaatcg attgctggcc tgcgagctgg tgcgtacgag   3600

gaaactgtct ctgaacagaa gaaggaggac gtctctgact acgagaactc ccagtacaag   3660

gagttcctag tcccctctcc caacgagaag ctggccagag gtctgctcat gctggccgag   3720

ctgtcttgca agggctctct ggccactggc gagtactcca agcagaccat tgagcttgcc   3780

cgatccgacc ccgagtttgt ggttggcttc attgcccaga accgacctaa gggcgactct   3840

gaggactggc ttattctgac ccccggggtg ggtcttgacg acaagggaga cgctctcgga   3900

cagcagtacc gaactgttga ggatgtcatg tctaccggaa cggatatcat aattgtcggc   3960

cgaggtctgt acggccagaa ccgagatcct attgaggagg ccaagcgata ccagaaggct   4020

ggctgggagg cttaccagaa gattaactgt tagaggttag actatggata tgtaatttaa   4080

ctgtgtatat agagagcgtg caagtatgga gcgcttgttc agcttgtatg atggtcagac   4140

gacctgtctg atcgagtatg tatgatactg cacaacctgt gtatccgcat gatctgtcca   4200

atggggcatg ttgttgtgtt tctcgatacg gagatgctgg gtacagtgct aatacgttga   4260

actacttata cttatatgag gctcgaagaa agctgacttg tgtatgactt aat          4313


<210>  86
<211>  1459
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic mutant Ura3 gene, comprising a 33 bp deletion from +21 
       to +53, a  1 bp deletion at +376 and a 3 bp deletion from +400 to
       403 of the Yarrowia Ura3 coding region (GenBank Accession No. 
       AJ306421)

<400>  86
gagtatctgt ctgactcgtc attgccgcct ttggagtacg actccaacta tgagtgtgct     60

tggatcactt tgacgataca ttcttcgttg gaggctgtgg gtctgacagc tgcgttttcg    120

gcgcggttgg ccgacaacaa tatcagctgc aacgtcattg ctggctttca tcatgatcac    180

atttttgtcg gcaaaggcga cgcccagaga gccattgacg ttctttctaa tttggaccga    240

tagccgtata gtccagtcta tctataagtt caactaactc gtaactatta ccataacata    300

tacttcactg ccccagataa ggttccgata aaaagttctg cagactaaat ttatttcagt    360

ctcctcttca ccaccaaaat gccctcctac gaagctcgag tgctcaagct cgtggcagcc    420

aagaaaacca acctgtgtgc ttctctggat gttaccacca ccaaggagct cattgagctt    480

gccgataagg tcggacctta tgtgtgcatg atcaaaaccc atatcgacat cattgacgac    540

ttcacctacg ccggcactgt gctccccctc aaggaacttg ctcttaagca cggtttcttc    600

ctgttcgagg acagaaagtt cgcagatatt ggcaacactg tcaagcacca gtaccggtgt    660

caccgaatcg ccgagtggtc cgatatcacc aacgcccacg gtgtacccgg aaccggaatc    720

gattgctggc ctgcgagctg gtgcgtacga ggaaactgtc tctgaacaga agaaggagga    780

cgtctctgac tacgagaact cccagtacaa ggagttccta gtcccctctc ccaacgagaa    840

gctggccaga ggtctgctca tgctggccga gctgtcttgc aagggctctc tggccactgg    900

cgagtactcc aagcagacca ttgagcttgc ccgatccgac cccgagtttg tggttggctt    960

cattgcccag aaccgaccta agggcgactc tgaggactgg cttattctga cccccggggt   1020

gggtcttgac gacaagggag acgctctcgg acagcagtac cgaactgttg aggatgtcat   1080

gtctaccgga acggatatca taattgtcgg ccgaggtctg tacggccaga accgagatcc   1140

tattgaggag gccaagcgat accagaaggc tggctgggag gcttaccaga agattaactg   1200

ttagaggtta gactatggat atgtaattta actgtgtata tagagagcgt gcaagtatgg   1260

agcgcttgtt cagcttgtat gatggtcaga cgacctgtct gatcgagtat gtatgatact   1320

gcacaacctg tgtatccgca tgatctgtcc aatggggcat gttgttgtgt ttctcgatac   1380

ggagatgctg ggtacagtgc taatacgttg aactacttat acttatatga ggctcgaaga   1440

aagctgactt gtgtatgac                                                1459


<210>  87
<211>  7966
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pYPS161

<400>  87
aaatgtaacg aaactgaaat ttgaccagat attgtgtccg cggtggagct ccagcttttg     60

ttccctttag tgagggttaa tttcgagctt ggcgtaatca tggtcatagc tgtttcctgt    120

gtgaaattgt tatccgctca caagcttcca cacaacgtac gttctggttg gctcggatga    180

tttctgcggc cccagcgtaa ggcaggcgtt ccgtccggat cggtttgggt cggatcggct    240

ttttgattgt cgtattgtcg ctcatgttgg acctggtgtg tagttgtagt gtcagatcag    300

attcaccagc gaatgcatgt gaacttcccc acattttgag ccgaggcaga tttgggttgc    360

ttagtaagca gacgtggcgt tgcaagtaga tgtggcaaat ggggacgaag attccgaggg    420

gatatcatag ttccaagggg atgtcatcat ttgccagctt tcgccgccac ttttgacgag    480

tttttgtggg tcaaataagt ttagttgaac ttttcaaatt tcagttggca ttttgttaat    540

agaaagggtg ccggtgctgg ggggttcatt cctcgggttg cagatatcct atctgtctta    600

ggggtatctc tttcaatcga caagatgtag ttgggtaaca attatttatt aatattctct    660

ccatccagta cagtactaac atcttgacat ctcagcacaa gtgcatcttc ccaagtgttt    720

gttggagagg ttgttgggta ttacttagga aacagaacac agtacgtgga gatcttggat    780

acatcgtaca tggaggttat ccataaaaaa gaccctccag gactagttac aatgccgtta    840

gatgaggaaa tccacaaccc tgattcacta tgaacatatt atcttccccc aaacttgcga    900

tatatggccc ttgatgatag ccttgatttt acccttgatg gtacctccac gaccaaccga    960

tctgctgttt gaagagatat tttcaaattt gaagtgctca gatctactaa acatgagtcc   1020

agtaattctt tccgtctttc cgatttccga tattcccttt tttagcccga cttttcactg   1080

ctcccatgtc aaacgattag gacttgggag acaatcccac tgtcaaaatc accccgatat   1140

tctctgtaaa acaagtactt cttccacgtg atcttcaaat acctcttcca cgtgaccttc   1200

aaatacctct tcaagtacct cttccacgcg accttcaaag tcccttcaaa tacccttctc   1260

aattctcccc ttctcctcca tagtccttct ctctgactaa gcttgagaat acatgacgct   1320

aagacgaaaa cacactagag accctgagag cctgaacatg catccactct gcagttgcgc   1380

acgtgcctac agcaactatc gggtccagtg ctggatctga cactgcgtct ccctatgaag   1440

aaactgataa acagatctgc actcataaca atgatctgag cgatgaaaac gtgacctcca   1500

cagccacaag tcataatcgg cgcgccagct gcattaatga atcggccaac gcgcggggag   1560

aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt   1620

cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga   1680

atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg   1740

taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa   1800

aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt   1860

tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct   1920

gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct   1980

cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc   2040

cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt   2100

atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc   2160

tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat   2220

ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa   2280

acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa   2340

aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga   2400

aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct   2460

tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga   2520

cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc   2580

catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg   2640

ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat   2700

aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat   2760

ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg   2820

caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc   2880

attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa   2940

agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc   3000

actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt   3060

ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag   3120

ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt   3180

gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag   3240

atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac   3300

cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc   3360

gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca   3420

gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg   3480

ggttccgcgc acatttcccc gaaaagtgcc acctgatgcg gtgtgaaata ccgcacagat   3540

gcgtaaggag aaaataccgc atcaggaaat tgtaagcgtt aatattttgt taaaattcgc   3600

gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc   3660

ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag   3720

tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga   3780

tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc   3840

actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa   3900

cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt   3960

agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc   4020

gtccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg   4080

ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca   4140

gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta   4200

tagggcgaat tgggcccgac gtcgcatgca actattagtg aggcttcggg agtggttgtc   4260

tcggttgtct cattcagact cgttgtgttg tatctatatc tatataaaca ctcttgtccc   4320

tcaatcccac tgccatcttt tgctaaactt gccgccaata tgaaactcat ctccctcatc   4380

accgtcgcta ccaccgctct ggcggctgtc ggagacaagt acaagctgac ctataccaga   4440

tcagacgccc aatcggtcga atctctgccc gtcacctacc aagatgacct gatcaccgcc   4500

tccaccgacg gcgaacccat caccatcacc gagggcgagg gcaacacctt ctctgttaac   4560

gacatgccca tcgcctatct ggagctgcag gctttgttct ggaccggcga ctacggctac   4620

aagctccagg gctcggtctt tgacattgcc gccgatggaa cctttgagct gagagacggc   4680

cccaaggagt actactattg cactcctcac cctgagcgaa acgtcatcta cgtcatcaac   4740

agccccgact actccaagtg tcggttcaag cgtaccatca agttccacgc tgaaaagatc   4800

taagtggtaa tcgaccgact aaccattttt agctgacaaa cacttgctaa ctcctataac   4860

gaatgaatga ctaacttggc atattgttac caagtattac ttgggatata gttgagtgta   4920

accattgcta agaatccaaa ctggagcttc taaaggtctg ggagtcgccg tatgtgttca   4980

tatcgaaatc aaagaaatca taatcgcaac agaattcaaa atcaagcaga ttaatatcca   5040

ttattgtact cggatcgtga catatctgat atgatctcgg atatgatctc tgactgttta   5100

ctgggagatt tgttgaagat ttgttgaggt tatctgaaaa gtagacaata gagacaaaat   5160

gacgatatca agaactgaat cgggccgaaa tactcggtat cattcccttc agcagtaact   5220

gtattgctct atcaatgcga cgagatacct ccacaattaa tactgtatac gctctaccac   5280

tcatatctcc aatgctaaaa tatattcatg cccaggacct ctgtgcactg ctatgcagca   5340

cagtgttgtc gattgaattg gtcgtgtctg gtccctgatg ctctgtgtct cgctgactag   5400

tccttccatc cagacctcgt cattatctga taggcaacaa gttctgctct ctcacaccct   5460

gccgacacaa gggacactcg ggcttctctc tcacccattc ggaaatacag tccttaatta   5520

agttgcgaca catgtcttga tagtatcttg aattctctct cttgagcttt tccataacaa   5580

gttcttctgc ctccaggaag tccatgggtg gtttgatcat ggttttggtg tagtggtagt   5640

gcagtggtgg tattgtgact ggggatgtag ttgagaataa gtcatacaca agtcagcttt   5700

cttcgagcct catataagta taagtagttc aacgtattag cactgtaccc agcatctccg   5760

tatcgagaaa cacaacaaca tgccccattg gacagatcat gcggatacac aggttgtgca   5820

gtatcataca tactcgatca gacaggtcgt ctgaccatca tacaagctga acaagcgctc   5880

catacttgca cgctctctat atacacagtt aaattacata tccatagtct aacctctaac   5940

agttaatctt ctggtaagcc tcccagccag ccttctggta tcgcttggcc tcctcaatag   6000

gatctcggtt ctggccgtac agacctcggc cgacaattat gatatccgtt ccggtagaca   6060

tgacatcctc aacagttcgg tactgctgtc cgagagcgtc tcccttgtcg tcaagaccca   6120

ccccgggggt cagaataagc cagtcctcag agtcgccctt aggtcggttc tgggcaatga   6180

agccaaccac aaactcgggg tcggatcggg caagctcaat ggtctgcttg gagtactcgc   6240

cagtggccag agagcccttg caagacagct cggccagcat gagcagacct ctggccagct   6300

tctcgttggg agaggggact aggaactcct tgtactggga gttctcgtag tcagagacgt   6360

cctccttctt ctgttcagag acagtttcct cggcaccagc tcgcaggcca gcaatgattc   6420

cggttccggg tacaccgtgg gcgttggtga tatcggacca ctcggcgatt cggtgacacc   6480

ggtactggtg cttgacagtg ttgccaatat ctgcgaactt tctgtcctcg aacaggaaga   6540

aaccgtgctt aagagcaagt tccttgaggg ggagcacagt gccggcgtag gtgaagtcgt   6600

caatgatgtc gatatgggtt ttgatcatgc acacataagg tccgacctta tcggcaagct   6660

caatgagctc cttggtggtg gtaacatcca gagaagcaca caggttggtt ttcttggctg   6720

ccacgagctt gagcactcga gcggcaaagg cggacttgtg gacgttagct cgagcttcgt   6780

aggagggcat tttggtggtg aagaggagac tgaaataaat ttagtctgca gaacttttta   6840

tcggaacctt atctggggca gtgaagtata tgttatggta atagttacga gttagttgaa   6900

cttatagata gactggacta tacggctatc ggtccaaatt agaaagaacg tcaatggctc   6960

tctgggcgtc gcctttgccg acaaaaatgt gatcatgatg aaagccagca atgacgttgc   7020

agctgatatt gttgtcggcc aaccgcgccg aaaacgcagc tgtcagaccc acagcctcca   7080

acgaagaatg tatcgtcaaa gtgatccaag cacactcata gttggagtcg tactccaaag   7140

gcggcaatga cgagtcagac agatactcgt cgaccttttc cttgggaacc accaccgtca   7200

gcccttctga ctcacgtatt gtagccaccg acacaggcaa cagtccgtgg atagcagaat   7260

atgtcttgtc ggtccatttc tcaccaactt taggcgtcaa gtgaatgttg cagaagaagt   7320

atgtgccttc attgagaatc ggtgttgctg atttcaataa agtcttgaga tcagtttggc   7380

cagtcatgtt gtggggggta attggattga gttatcgcct acagtctgta caggtatact   7440

cgctgcccac tttatacttt ttgattccgc tgcacttgaa gcaatgtcgt ttaccaaaag   7500

tgagaatgct ccacagaaca caccccaggg tatggttgag caaaaaataa acactccgat   7560

acggggaatc gaaccccggt ctccacggtt ctcaagaagt attcttgatg agagcgtatc   7620

gatgagccta aaatgaaccc gagtatatct cataaaattc tcggtgagag gtctgtgact   7680

gtcagtacaa ggtgccttca ttatgccctc aaccttacca tacctcactg aatgtagtgt   7740

acctctaaaa atgaaataca gtgccaaaag ccaaggcact gagctcgtct aacggacttg   7800

atatacaacc aattaaaaca aatgaaaaga aatacagttc tttgtatcat ttgtaacaat   7860

taccctgtac aaactaaggt attgaaatcc cacaatattc ccaaagtcca cccctttcca   7920

aattgtcatg cctacaactc atataccaag cactaaccta ccgttt                  7966


<210>  88
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Pex-10del1 3'.Forward

<400>  88
ccaacatgag cgacaatacg                                                 20


<210>  89
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Pex-10del2 5'.Reverse

<400>  89
caagttctgc tctctcacac                                                 20


