                         SEQUENCE LISTING

<110>  E. I. du Pont de Nemours & Co., Inc.
       
 
<120>  OPTIMIZED STRAINS OF YARROWIA LIPOLYTICA FOR HIGH 
       EICOSAPENTAENOIC ACID PRODUCTION

<130>  CL3941

<160>  201   

<170>  PatentIn version 3.4

<210>  1
<211>  1936
<212>  DNA
<213>  Yarrowia lipolytica


<220>
<221>  CDS
<222>  (283)..(1539)

<300>
<302>  A  DELTA-12 DESATURASE GENE SUITABLE FOR ALTERING LEVELS OF 
       POLYUNSATURATED FATTY ACIDS IN OLEAGINOUS YEASTS
<310>  U.S. 7,214,491
<311>  2004-05-06
<312>  2007-05-08
<313>  (1)..(1936)

<300>
<302>  A  DELTA-12 DESATURASE GENE SUITABLE FOR ALTERING LEVELS OF 
       POLYUNSATURATED FATTY ACIDS IN OLEAGINOUS YEASTS
<310>  WO 2004/104167
<311>  2004-05-07
<312>  2004-12-02
<313>  (1)..(1936)

<400>  1
cgtagttata tacaagaggt agatgcgtgc tggtgttaga ggggctctca ggattaggag     60

gaaaatttga cattggccct caacatataa cctcgggtgt gcctctgttt accctcagct    120

tttgcttgtc cccaagtcag tcacgccagg ccaaaaaggt tggtggattg acagggagaa    180

aaaaaaaagc ctagtgggtt taaactcgag gtaagacatt gaaatatata ccggtcggca    240

tcctgagtcc ctttctcgta ttccaacaga ccgaccatag aa atg gat tcg acc       294
                                               Met Asp Ser Thr          
                                               1                        

acg cag acc aac acc ggc acc ggc aag gtg gcc gtg cag ccc ccc acg      342
Thr Gln Thr Asn Thr Gly Thr Gly Lys Val Ala Val Gln Pro Pro Thr         
5                   10                  15                  20          

gcc ttc att aag ccc att gag aag gtg tcc gag ccc gtc tac gac acc      390
Ala Phe Ile Lys Pro Ile Glu Lys Val Ser Glu Pro Val Tyr Asp Thr         
                25                  30                  35              

ttt ggc aac gag ttc act cct cca gac tac tct atc aag gat att ctg      438
Phe Gly Asn Glu Phe Thr Pro Pro Asp Tyr Ser Ile Lys Asp Ile Leu         
            40                  45                  50                  

gat gcc att ccc cag gag tgc tac aag cgg tcc tac gtt aag tcc tac      486
Asp Ala Ile Pro Gln Glu Cys Tyr Lys Arg Ser Tyr Val Lys Ser Tyr         
        55                  60                  65                      

tcg tac gtg gcc cga gac tgc ttc ttt atc gcc gtt ttt gcc tac atg      534
Ser Tyr Val Ala Arg Asp Cys Phe Phe Ile Ala Val Phe Ala Tyr Met         
    70                  75                  80                          

gcc tac gcg tac ctg cct ctt att ccc tcg gct tcc ggc cga gct gtg      582
Ala Tyr Ala Tyr Leu Pro Leu Ile Pro Ser Ala Ser Gly Arg Ala Val         
85                  90                  95                  100         

gcc tgg gcc atg tac tcc att gtc cag ggt ctg ttt ggc acc ggt ctg      630
Ala Trp Ala Met Tyr Ser Ile Val Gln Gly Leu Phe Gly Thr Gly Leu         
                105                 110                 115             

tgg gtt ctt gcc cac gag tgt ggc cac tct gct ttc tcc gac tct aac      678
Trp Val Leu Ala His Glu Cys Gly His Ser Ala Phe Ser Asp Ser Asn         
            120                 125                 130                 

acc gtc aac aac gtc acc gga tgg gtt ctg cac tcc tcc atg ctg gtc      726
Thr Val Asn Asn Val Thr Gly Trp Val Leu His Ser Ser Met Leu Val         
        135                 140                 145                     

cct tac tac gcc tgg aag ctg acc cac tcc atg cac cac aag tcc act      774
Pro Tyr Tyr Ala Trp Lys Leu Thr His Ser Met His His Lys Ser Thr         
    150                 155                 160                         

ggt cac ctc acc cgt gat atg gtg ttt gtg ccc aag gac cga aag gag      822
Gly His Leu Thr Arg Asp Met Val Phe Val Pro Lys Asp Arg Lys Glu         
165                 170                 175                 180         

ttt atg gag aac cga ggc gcc cat gac tgg tct gag ctt gct gag gac      870
Phe Met Glu Asn Arg Gly Ala His Asp Trp Ser Glu Leu Ala Glu Asp         
                185                 190                 195             

gct ccc ctc atg acc ctc tac ggc ctc atc acc cag cag gtg ttt gga      918
Ala Pro Leu Met Thr Leu Tyr Gly Leu Ile Thr Gln Gln Val Phe Gly         
            200                 205                 210                 

tgg cct ctg tat ctg ctg tct tac gtt acc gga cag aag tac ccc aag      966
Trp Pro Leu Tyr Leu Leu Ser Tyr Val Thr Gly Gln Lys Tyr Pro Lys         
        215                 220                 225                     

ctc aac aaa tgg gct gtc aac cac ttc aac ccc aac gcc ccg ctg ttt     1014
Leu Asn Lys Trp Ala Val Asn His Phe Asn Pro Asn Ala Pro Leu Phe         
    230                 235                 240                         

gag aag aag gac tgg ttc aac atc tgg atc tct aac gtc ggt att ggt     1062
Glu Lys Lys Asp Trp Phe Asn Ile Trp Ile Ser Asn Val Gly Ile Gly         
245                 250                 255                 260         

atc acc atg tcc gtc atc gca tac tcc atc aac cga tgg ggc ctg gct     1110
Ile Thr Met Ser Val Ile Ala Tyr Ser Ile Asn Arg Trp Gly Leu Ala         
                265                 270                 275             

tcc gtc acc ctc tac tac ctg atc ccc tac ctg tgg gtc aac cac tgg     1158
Ser Val Thr Leu Tyr Tyr Leu Ile Pro Tyr Leu Trp Val Asn His Trp         
            280                 285                 290                 

ctc gtg gcc atc acc tac ctg cag cac acc gac ccc act ctg ccc cac     1206
Leu Val Ala Ile Thr Tyr Leu Gln His Thr Asp Pro Thr Leu Pro His         
        295                 300                 305                     

tac cac gcc gac cag tgg aac ttc acc cga gga gcc gcc gcc acc atc     1254
Tyr His Ala Asp Gln Trp Asn Phe Thr Arg Gly Ala Ala Ala Thr Ile         
    310                 315                 320                         

gac cga gag ttt ggc ttc atc ggc tcc ttc tgc ttc cat gac atc atc     1302
Asp Arg Glu Phe Gly Phe Ile Gly Ser Phe Cys Phe His Asp Ile Ile         
325                 330                 335                 340         

gag acc cac gtt ctg cac cac tac gtg tct cga att ccc ttc tac aac     1350
Glu Thr His Val Leu His His Tyr Val Ser Arg Ile Pro Phe Tyr Asn         
                345                 350                 355             

gcc cga atc gcc act gag aag atc aag aag gtc atg ggc aag cac tac     1398
Ala Arg Ile Ala Thr Glu Lys Ile Lys Lys Val Met Gly Lys His Tyr         
            360                 365                 370                 

cga cac gac gac acc aac ttc atc aag tct ctt tac act gtc gcc cga     1446
Arg His Asp Asp Thr Asn Phe Ile Lys Ser Leu Tyr Thr Val Ala Arg         
        375                 380                 385                     

acc tgc cag ttt gtt gaa ggt aag gaa ggc att cag atg ttt aga aac     1494
Thr Cys Gln Phe Val Glu Gly Lys Glu Gly Ile Gln Met Phe Arg Asn         
    390                 395                 400                         

gtc aat gga gtc gga gtt gct cct gac ggc ctg cct tct aaa aag         1539
Val Asn Gly Val Gly Val Ala Pro Asp Gly Leu Pro Ser Lys Lys             
405                 410                 415                             

tagagctaga aatgttattt gattgtgttt taactgaaca gcaccgagcc cgaggctaag   1599

ccaagcgaag ccgaggggtt gtgtagtcca tggacgtaac gagtaggcga tatcaccgca   1659

ctcggcactg cgtgtctgcg ttcatgggcg aagtcacatt acgctgacaa ccgttgtagt   1719

ttccctttag tatcaatact gttacaagta ccggtctcgt actcgtactg atacgaatct   1779

gtgggaagaa gtcaccctta tcagaccttc atactgatgt ttcggatatc aatagaactg   1839

gcatagagcc gttaaagaag tttcacttaa tcactccaac cctcctactt gtagattcaa   1899

gcagatcgat aagatggatt tgatggtcag tgctagc                            1936


<210>  2
<211>  419
<212>  PRT
<213>  Yarrowia lipolytica

<400>  2

Met Asp Ser Thr Thr Gln Thr Asn Thr Gly Thr Gly Lys Val Ala Val 
1               5                   10                  15      


Gln Pro Pro Thr Ala Phe Ile Lys Pro Ile Glu Lys Val Ser Glu Pro 
            20                  25                  30          


Val Tyr Asp Thr Phe Gly Asn Glu Phe Thr Pro Pro Asp Tyr Ser Ile 
        35                  40                  45              


Lys Asp Ile Leu Asp Ala Ile Pro Gln Glu Cys Tyr Lys Arg Ser Tyr 
    50                  55                  60                  


Val Lys Ser Tyr Ser Tyr Val Ala Arg Asp Cys Phe Phe Ile Ala Val 
65                  70                  75                  80  


Phe Ala Tyr Met Ala Tyr Ala Tyr Leu Pro Leu Ile Pro Ser Ala Ser 
                85                  90                  95      


Gly Arg Ala Val Ala Trp Ala Met Tyr Ser Ile Val Gln Gly Leu Phe 
            100                 105                 110         


Gly Thr Gly Leu Trp Val Leu Ala His Glu Cys Gly His Ser Ala Phe 
        115                 120                 125             


Ser Asp Ser Asn Thr Val Asn Asn Val Thr Gly Trp Val Leu His Ser 
    130                 135                 140                 


Ser Met Leu Val Pro Tyr Tyr Ala Trp Lys Leu Thr His Ser Met His 
145                 150                 155                 160 


His Lys Ser Thr Gly His Leu Thr Arg Asp Met Val Phe Val Pro Lys 
                165                 170                 175     


Asp Arg Lys Glu Phe Met Glu Asn Arg Gly Ala His Asp Trp Ser Glu 
            180                 185                 190         


Leu Ala Glu Asp Ala Pro Leu Met Thr Leu Tyr Gly Leu Ile Thr Gln 
        195                 200                 205             


Gln Val Phe Gly Trp Pro Leu Tyr Leu Leu Ser Tyr Val Thr Gly Gln 
    210                 215                 220                 


Lys Tyr Pro Lys Leu Asn Lys Trp Ala Val Asn His Phe Asn Pro Asn 
225                 230                 235                 240 


Ala Pro Leu Phe Glu Lys Lys Asp Trp Phe Asn Ile Trp Ile Ser Asn 
                245                 250                 255     


Val Gly Ile Gly Ile Thr Met Ser Val Ile Ala Tyr Ser Ile Asn Arg 
            260                 265                 270         


Trp Gly Leu Ala Ser Val Thr Leu Tyr Tyr Leu Ile Pro Tyr Leu Trp 
        275                 280                 285             


Val Asn His Trp Leu Val Ala Ile Thr Tyr Leu Gln His Thr Asp Pro 
    290                 295                 300                 


Thr Leu Pro His Tyr His Ala Asp Gln Trp Asn Phe Thr Arg Gly Ala 
305                 310                 315                 320 


Ala Ala Thr Ile Asp Arg Glu Phe Gly Phe Ile Gly Ser Phe Cys Phe 
                325                 330                 335     


His Asp Ile Ile Glu Thr His Val Leu His His Tyr Val Ser Arg Ile 
            340                 345                 350         


Pro Phe Tyr Asn Ala Arg Ile Ala Thr Glu Lys Ile Lys Lys Val Met 
        355                 360                 365             


Gly Lys His Tyr Arg His Asp Asp Thr Asn Phe Ile Lys Ser Leu Tyr 
    370                 375                 380                 


Thr Val Ala Arg Thr Cys Gln Phe Val Glu Gly Lys Glu Gly Ile Gln 
385                 390                 395                 400 


Met Phe Arg Asn Val Asn Gly Val Gly Val Ala Pro Asp Gly Leu Pro 
                405                 410                 415     


Ser Lys Lys 
            


<210>  3
<211>  10
<212>  DNA
<213>  Yarrowia lipolytica


<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  n is a, c, g, or t

<300>
<302>  PRODUCTION OF POLYUNSATURATED FATTY ACIDS IN OLEAGINOUS YEASTS
<310>  U.S. 7,238,482
<311>  2004-05-06
<312>  2007-07-03
<313>  (1)..(10)

<300>
<302>  PRODUCTION OF POLYUNSATURATED FATTY ACIDS IN OLEAGINOUS YEASTS
<310>  WO 2004/101757
<311>  2004-05-07
<312>  2004-11-25
<313>  (1)..(10)

<400>  3
mammatgnhs                                                            10


<210>  4
<211>  777
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(777)
<223>  delta-9 elongase

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  WO 2007/061742
<311>  2006-11-16
<312>  2007-05-31
<313>  (1)..(777)

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  US 2007-0117190-A1
<311>  2006-11-16
<312>  2007-05-24
<313>  (1)..(777)

<400>  4
atg gag gtg gtg aat gaa ata gtc tca att ggg cag gaa gtt tta ccc       48
Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro         
1               5                   10                  15              

aaa gtt gat tat gcc caa ctc tgg agt gat gcc agt cac tgt gag gtg       96
Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val         
            20                  25                  30                  

ctt tac ttg tcc atc gca ttt gtc atc ttg aag ttc act ctt ggc ccc      144
Leu Tyr Leu Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro         
        35                  40                  45                      

ctt ggt cca aaa ggt cag tct cgt atg aag ttt gtt ttc acc aat tac      192
Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr         
    50                  55                  60                          

aac ctt ctc atg tcc att tat tcg ttg gga tca ttc ctc tca atg gca      240
Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala         
65                  70                  75                  80          

tat gcc atg tac acc atc ggt gtt atg tct gac aac tgc gag aag gct      288
Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala         
                85                  90                  95              

ttt gac aac aac gtc ttc agg atc acc acg cag ttg ttc tat ttg agc      336
Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser         
            100                 105                 110                 

aag ttc ctg gag tat att gac tcc ttc tat ttg cca ctg atg ggc aag      384
Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys         
        115                 120                 125                     

cct ctg acc tgg ttg caa ttc ttc cat cat ttg ggg gca ccg atg gat      432
Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp         
    130                 135                 140                         

atg tgg ctg ttc tat aat tac cga aat gaa gct gtt tgg att ttt gtg      480
Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val         
145                 150                 155                 160         

ctg ttg aat ggt ttc atc cac tgg atc atg tac ggt tat tat tgg acc      528
Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr         
                165                 170                 175             

aga ttg atc aag ctg aag ttc ccc atg cca aaa tcc ctg att aca tca      576
Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser         
            180                 185                 190                 

atg cag atc att caa ttc aat gtt ggt ttc tac att gtc tgg aag tac      624
Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr         
        195                 200                 205                     

agg aac att ccc tgt tat cgc caa gat ggg atg agg atg ttt ggc tgg      672
Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp         
    210                 215                 220                         

ttc ttc aat tac ttt tat gtt ggc aca gtc ttg tgt ttg ttc ttg aat      720
Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn         
225                 230                 235                 240         

ttc tat gtg caa acg tat atc gtc agg aag cac aag gga gcc aaa aag      768
Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys         
                245                 250                 255             

att cag tga                                                          777
Ile Gln                                                                 
                                                                        


<210>  5
<211>  258
<212>  PRT
<213>  Euglena gracilis

<400>  5

Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro 
1               5                   10                  15      


Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val 
            20                  25                  30          


Leu Tyr Leu Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro 
        35                  40                  45              


Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr 
    50                  55                  60                  


Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala 
65                  70                  75                  80  


Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala 
                85                  90                  95      


Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser 
            100                 105                 110         


Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys 
        115                 120                 125             


Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp 
    130                 135                 140                 


Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val 
145                 150                 155                 160 


Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr 
                165                 170                 175     


Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 
        195                 200                 205             


Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp 
    210                 215                 220                 


Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys 
                245                 250                 255     


Ile Gln 
        


<210>  6
<211>  777
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(777)
<223>  synthetic delta-9 elongase (codon-optimized for Yarrowia 
       lipolytica)

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  WO 2007/061742
<311>  2006-11-16
<312>  2007-05-31
<313>  (1)..(777)

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  US-2007-0117190-A1
<311>  2006-11-16
<312>  2007-05-24
<313>  (1)..(777)

<400>  6
atg gag gtc gtg aac gaa atc gtc tcc att ggc cag gag gtt ctt ccc       48
Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro         
1               5                   10                  15              

aag gtc gac tat gct cag ctc tgg tct gat gcc tcg cac tgc gag gtg       96
Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val         
            20                  25                  30                  

ctg tac ctc tcc atc gcc ttc gtc atc ctg aag ttc acc ctt ggt cct      144
Leu Tyr Leu Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro         
        35                  40                  45                      

ctc gga ccc aag ggt cag tct cga atg aag ttt gtg ttc acc aac tac      192
Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr         
    50                  55                  60                          

aac ctg ctc atg tcc atc tac tcg ctg ggc tcc ttc ctc tct atg gcc      240
Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala         
65                  70                  75                  80          

tac gcc atg tac acc att ggt gtc atg tcc gac aac tgc gag aag gct      288
Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala         
                85                  90                  95              

ttc gac aac aat gtc ttc cga atc acc act cag ctg ttc tac ctc agc      336
Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser         
            100                 105                 110                 

aag ttc ctc gag tac att gac tcc ttc tat ctg ccc ctc atg ggc aag      384
Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys         
        115                 120                 125                     

cct ctg acc tgg ttg cag ttc ttt cac cat ctc gga gct cct atg gac      432
Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp         
    130                 135                 140                         

atg tgg ctg ttc tac aac tac cga aac gaa gcc gtt tgg atc ttt gtg      480
Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val         
145                 150                 155                 160         

ctg ctc aac ggc ttc att cac tgg atc atg tac ggc tac tat tgg acc      528
Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr         
                165                 170                 175             

cga ctg atc aag ctc aag ttc cct atg ccc aag tcc ctg att act tct      576
Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser         
            180                 185                 190                 

atg cag atc att cag ttc aac gtt ggc ttc tac atc gtc tgg aag tac      624
Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr         
        195                 200                 205                     

cgg aac att ccc tgc tac cga caa gat gga atg aga atg ttt ggc tgg      672
Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp         
    210                 215                 220                         

ttt ttc aac tac ttc tac gtt ggt act gtc ctg tgt ctg ttc ctc aac      720
Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn         
225                 230                 235                 240         

ttc tac gtg cag acc tac atc gtc cga aag cac aag gga gcc aaa aag      768
Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys         
                245                 250                 255             

att cag tga                                                          777
Ile Gln                                                                 
                                                                        


<210>  7
<211>  258
<212>  PRT
<213>  Euglena gracilis

<400>  7

Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro 
1               5                   10                  15      


Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val 
            20                  25                  30          


Leu Tyr Leu Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro 
        35                  40                  45              


Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr 
    50                  55                  60                  


Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala 
65                  70                  75                  80  


Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala 
                85                  90                  95      


Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser 
            100                 105                 110         


Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys 
        115                 120                 125             


Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp 
    130                 135                 140                 


Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val 
145                 150                 155                 160 


Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr 
                165                 170                 175     


Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 
        195                 200                 205             


Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp 
    210                 215                 220                 


Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys 
                245                 250                 255     


Ile Gln 
        


<210>  8
<211>  792
<212>  DNA
<213>  Eutreptiella sp. CCMP389


<220>
<221>  CDS
<222>  (1)..(792)
<223>  delta-9 elongase

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  WO 2007/061742
<311>  2006-11-16
<312>  2007-05-31
<313>  (1)..(792)

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  US 2007-0117190-A1
<311>  2006-11-16
<312>  2007-05-24
<313>  (1)..(792)

<400>  8
atg gct gcg gtg ata gag gtc gcc aac gag ttt gta gcc atc acg gca       48
Met Ala Ala Val Ile Glu Val Ala Asn Glu Phe Val Ala Ile Thr Ala         
1               5                   10                  15              

gaa acg ctc ccc aaa gtt gac tat caa cga cta tgg cga gac att tac       96
Glu Thr Leu Pro Lys Val Asp Tyr Gln Arg Leu Trp Arg Asp Ile Tyr         
            20                  25                  30                  

agt tgt gag cta ctg tat ttc tcc att gcc ttc gtg atc ttg aag ttt      144
Ser Cys Glu Leu Leu Tyr Phe Ser Ile Ala Phe Val Ile Leu Lys Phe         
        35                  40                  45                      

acg ttg ggc gag ttg agc gac agc gga aaa aag att ttg aga gtg ttg      192
Thr Leu Gly Glu Leu Ser Asp Ser Gly Lys Lys Ile Leu Arg Val Leu         
    50                  55                  60                          

ttc aag tgg tac aat ctc ttc atg tcc gtg ttc tcc ttg gtg tct ttc      240
Phe Lys Trp Tyr Asn Leu Phe Met Ser Val Phe Ser Leu Val Ser Phe         
65                  70                  75                  80          

ctt tgc atg ggc tat gcc att tat acc gtg ggc cta tac tct aac gaa      288
Leu Cys Met Gly Tyr Ala Ile Tyr Thr Val Gly Leu Tyr Ser Asn Glu         
                85                  90                  95              

tgc gac agg gct ttc gac aac tcg ttg ttc cgc ttt gca aca aag gtg      336
Cys Asp Arg Ala Phe Asp Asn Ser Leu Phe Arg Phe Ala Thr Lys Val         
            100                 105                 110                 

ttc tac tac agt aag ttt ttg gag tac atc gac tct ttt tat ctt ccg      384
Phe Tyr Tyr Ser Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro         
        115                 120                 125                     

ctc atg gcc aag ccg ctg tct ttc ctg caa ttc ttc cat cac ttg gga      432
Leu Met Ala Lys Pro Leu Ser Phe Leu Gln Phe Phe His His Leu Gly         
    130                 135                 140                         

gcc ccc atg gac atg tgg ctc ttt gtc caa tat tct ggg gaa tct att      480
Ala Pro Met Asp Met Trp Leu Phe Val Gln Tyr Ser Gly Glu Ser Ile         
145                 150                 155                 160         

tgg atc ttt gtg ttt ttg aat ggg ttc att cac ttt gtt atg tac ggg      528
Trp Ile Phe Val Phe Leu Asn Gly Phe Ile His Phe Val Met Tyr Gly         
                165                 170                 175             

tac tac tgg act cgg ctg atg aag ttc aat ttc cca atg ccc aag cag      576
Tyr Tyr Trp Thr Arg Leu Met Lys Phe Asn Phe Pro Met Pro Lys Gln         
            180                 185                 190                 

ttg att acc gcg atg cag atc acg cag ttc aac gtt ggt ttc tac ctc      624
Leu Ile Thr Ala Met Gln Ile Thr Gln Phe Asn Val Gly Phe Tyr Leu         
        195                 200                 205                     

gtg tgg tgg tac aaa gat att ccc tgc tac cga aag gat ccc atg cga      672
Val Trp Trp Tyr Lys Asp Ile Pro Cys Tyr Arg Lys Asp Pro Met Arg         
    210                 215                 220                         

atg ttg gcc tgg atc ttc aat tac tgg tat gtt ggg act gtc ttg ctg      720
Met Leu Ala Trp Ile Phe Asn Tyr Trp Tyr Val Gly Thr Val Leu Leu         
225                 230                 235                 240         

ctg ttc att aat ttc ttc gtc aaa tcc tat gtg ttc cca aag ccg aag      768
Leu Phe Ile Asn Phe Phe Val Lys Ser Tyr Val Phe Pro Lys Pro Lys         
                245                 250                 255             

act gca gat aaa aag gtc caa tag                                      792
Thr Ala Asp Lys Lys Val Gln                                             
            260                                                         


<210>  9
<211>  263
<212>  PRT
<213>  Eutreptiella sp. CCMP389

<400>  9

Met Ala Ala Val Ile Glu Val Ala Asn Glu Phe Val Ala Ile Thr Ala 
1               5                   10                  15      


Glu Thr Leu Pro Lys Val Asp Tyr Gln Arg Leu Trp Arg Asp Ile Tyr 
            20                  25                  30          


Ser Cys Glu Leu Leu Tyr Phe Ser Ile Ala Phe Val Ile Leu Lys Phe 
        35                  40                  45              


Thr Leu Gly Glu Leu Ser Asp Ser Gly Lys Lys Ile Leu Arg Val Leu 
    50                  55                  60                  


Phe Lys Trp Tyr Asn Leu Phe Met Ser Val Phe Ser Leu Val Ser Phe 
65                  70                  75                  80  


Leu Cys Met Gly Tyr Ala Ile Tyr Thr Val Gly Leu Tyr Ser Asn Glu 
                85                  90                  95      


Cys Asp Arg Ala Phe Asp Asn Ser Leu Phe Arg Phe Ala Thr Lys Val 
            100                 105                 110         


Phe Tyr Tyr Ser Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro 
        115                 120                 125             


Leu Met Ala Lys Pro Leu Ser Phe Leu Gln Phe Phe His His Leu Gly 
    130                 135                 140                 


Ala Pro Met Asp Met Trp Leu Phe Val Gln Tyr Ser Gly Glu Ser Ile 
145                 150                 155                 160 


Trp Ile Phe Val Phe Leu Asn Gly Phe Ile His Phe Val Met Tyr Gly 
                165                 170                 175     


Tyr Tyr Trp Thr Arg Leu Met Lys Phe Asn Phe Pro Met Pro Lys Gln 
            180                 185                 190         


Leu Ile Thr Ala Met Gln Ile Thr Gln Phe Asn Val Gly Phe Tyr Leu 
        195                 200                 205             


Val Trp Trp Tyr Lys Asp Ile Pro Cys Tyr Arg Lys Asp Pro Met Arg 
    210                 215                 220                 


Met Leu Ala Trp Ile Phe Asn Tyr Trp Tyr Val Gly Thr Val Leu Leu 
225                 230                 235                 240 


Leu Phe Ile Asn Phe Phe Val Lys Ser Tyr Val Phe Pro Lys Pro Lys 
                245                 250                 255     


Thr Ala Asp Lys Lys Val Gln 
            260             


<210>  10
<211>  792
<212>  DNA
<213>  Eutreptiella sp. CCMP389


<220>
<221>  CDS
<222>  (1)..(792)
<223>  synthetic delta-9 elongase (codon-optimized for Yarrowia 
       lipolytica)

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  WO 2007/061742
<311>  2006-11-16
<312>  2007-05-31
<313>  (1)..(792)

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  US 2007-0117190-A1
<311>  2006-11-16
<312>  2007-05-24
<313>  (1)..(792)

<400>  10
atg gct gcc gtc atc gag gtg gcc aac gag ttc gtc gct atc act gcc       48
Met Ala Ala Val Ile Glu Val Ala Asn Glu Phe Val Ala Ile Thr Ala         
1               5                   10                  15              

gag acc ctt ccc aag gtg gac tat cag cga ctc tgg cga gac atc tac       96
Glu Thr Leu Pro Lys Val Asp Tyr Gln Arg Leu Trp Arg Asp Ile Tyr         
            20                  25                  30                  

tcc tgc gag ctc ctg tac ttc tcc att gct ttc gtc atc ctc aag ttt      144
Ser Cys Glu Leu Leu Tyr Phe Ser Ile Ala Phe Val Ile Leu Lys Phe         
        35                  40                  45                      

acc ctt ggc gag ctc tcg gat tct ggc aaa aag att ctg cga gtg ctg      192
Thr Leu Gly Glu Leu Ser Asp Ser Gly Lys Lys Ile Leu Arg Val Leu         
    50                  55                  60                          

ttc aag tgg tac aac ctc ttc atg tcc gtc ttt tcg ctg gtg tcc ttc      240
Phe Lys Trp Tyr Asn Leu Phe Met Ser Val Phe Ser Leu Val Ser Phe         
65                  70                  75                  80          

ctc tgt atg ggt tac gcc atc tac acc gtt gga ctg tac tcc aac gaa      288
Leu Cys Met Gly Tyr Ala Ile Tyr Thr Val Gly Leu Tyr Ser Asn Glu         
                85                  90                  95              

tgc gac aga gct ttc gac aac agc ttg ttc cga ttt gcc acc aag gtc      336
Cys Asp Arg Ala Phe Asp Asn Ser Leu Phe Arg Phe Ala Thr Lys Val         
            100                 105                 110                 

ttc tac tat tcc aag ttt ctg gag tac atc gac tct ttc tac ctt ccc      384
Phe Tyr Tyr Ser Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro         
        115                 120                 125                     

ctc atg gcc aag cct ctg tcc ttt ctg cag ttc ttt cat cac ttg gga      432
Leu Met Ala Lys Pro Leu Ser Phe Leu Gln Phe Phe His His Leu Gly         
    130                 135                 140                         

gct cct atg gac atg tgg ctc ttc gtg cag tac tct ggc gaa tcc att      480
Ala Pro Met Asp Met Trp Leu Phe Val Gln Tyr Ser Gly Glu Ser Ile         
145                 150                 155                 160         

tgg atc ttt gtg ttc ctg aac gga ttc att cac ttt gtc atg tac ggc      528
Trp Ile Phe Val Phe Leu Asn Gly Phe Ile His Phe Val Met Tyr Gly         
                165                 170                 175             

tac tat tgg aca cgg ctg atg aag ttc aac ttt ccc atg ccc aag cag      576
Tyr Tyr Trp Thr Arg Leu Met Lys Phe Asn Phe Pro Met Pro Lys Gln         
            180                 185                 190                 

ctc att acc gca atg cag atc acc cag ttc aac gtt ggc ttc tac ctc      624
Leu Ile Thr Ala Met Gln Ile Thr Gln Phe Asn Val Gly Phe Tyr Leu         
        195                 200                 205                     

gtg tgg tgg tac aag gac att ccc tgt tac cga aag gat ccc atg cga      672
Val Trp Trp Tyr Lys Asp Ile Pro Cys Tyr Arg Lys Asp Pro Met Arg         
    210                 215                 220                         

atg ctg gcc tgg atc ttc aac tac tgg tac gtc ggt acc gtt ctt ctg      720
Met Leu Ala Trp Ile Phe Asn Tyr Trp Tyr Val Gly Thr Val Leu Leu         
225                 230                 235                 240         

ctc ttc atc aac ttc ttt gtc aag tcc tac gtg ttt ccc aag cct aag      768
Leu Phe Ile Asn Phe Phe Val Lys Ser Tyr Val Phe Pro Lys Pro Lys         
                245                 250                 255             

act gcc gac aaa aag gtc cag tag                                      792
Thr Ala Asp Lys Lys Val Gln                                             
            260                                                         


<210>  11
<211>  263
<212>  PRT
<213>  Eutreptiella sp. CCMP389

<400>  11

Met Ala Ala Val Ile Glu Val Ala Asn Glu Phe Val Ala Ile Thr Ala 
1               5                   10                  15      


Glu Thr Leu Pro Lys Val Asp Tyr Gln Arg Leu Trp Arg Asp Ile Tyr 
            20                  25                  30          


Ser Cys Glu Leu Leu Tyr Phe Ser Ile Ala Phe Val Ile Leu Lys Phe 
        35                  40                  45              


Thr Leu Gly Glu Leu Ser Asp Ser Gly Lys Lys Ile Leu Arg Val Leu 
    50                  55                  60                  


Phe Lys Trp Tyr Asn Leu Phe Met Ser Val Phe Ser Leu Val Ser Phe 
65                  70                  75                  80  


Leu Cys Met Gly Tyr Ala Ile Tyr Thr Val Gly Leu Tyr Ser Asn Glu 
                85                  90                  95      


Cys Asp Arg Ala Phe Asp Asn Ser Leu Phe Arg Phe Ala Thr Lys Val 
            100                 105                 110         


Phe Tyr Tyr Ser Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro 
        115                 120                 125             


Leu Met Ala Lys Pro Leu Ser Phe Leu Gln Phe Phe His His Leu Gly 
    130                 135                 140                 


Ala Pro Met Asp Met Trp Leu Phe Val Gln Tyr Ser Gly Glu Ser Ile 
145                 150                 155                 160 


Trp Ile Phe Val Phe Leu Asn Gly Phe Ile His Phe Val Met Tyr Gly 
                165                 170                 175     


Tyr Tyr Trp Thr Arg Leu Met Lys Phe Asn Phe Pro Met Pro Lys Gln 
            180                 185                 190         


Leu Ile Thr Ala Met Gln Ile Thr Gln Phe Asn Val Gly Phe Tyr Leu 
        195                 200                 205             


Val Trp Trp Tyr Lys Asp Ile Pro Cys Tyr Arg Lys Asp Pro Met Arg 
    210                 215                 220                 


Met Leu Ala Trp Ile Phe Asn Tyr Trp Tyr Val Gly Thr Val Leu Leu 
225                 230                 235                 240 


Leu Phe Ile Asn Phe Phe Val Lys Ser Tyr Val Phe Pro Lys Pro Lys 
                245                 250                 255     


Thr Ala Asp Lys Lys Val Gln 
            260             


<210>  12
<211>  774
<212>  DNA
<213>  Euglena anabaena UTEX 373


<220>
<221>  CDS
<222>  (1)..(774)
<223>  delta-9 elongase

<400>  12
atg gaa gca gcc aaa gaa ttg gtt tcc atc gtc caa gag gag ctc ccc       48
Met Glu Ala Ala Lys Glu Leu Val Ser Ile Val Gln Glu Glu Leu Pro         
1               5                   10                  15              

aag gtg gac tat gcc cag ctt tgg cag gat gcc agc agc tgt gag gtc       96
Lys Val Asp Tyr Ala Gln Leu Trp Gln Asp Ala Ser Ser Cys Glu Val         
            20                  25                  30                  

ctt tac ctc tcg gtg gca ttc gtg gcg atc aag ttc atg ctg cgc cca      144
Leu Tyr Leu Ser Val Ala Phe Val Ala Ile Lys Phe Met Leu Arg Pro         
        35                  40                  45                      

ctg gac ctg aag cgc cag gcc acc ttg aag aag ctg ttc aca gca tac      192
Leu Asp Leu Lys Arg Gln Ala Thr Leu Lys Lys Leu Phe Thr Ala Tyr         
    50                  55                  60                          

aac ttc ctc atg tcg atc tat tcc ttt ggc tcc ttc ctg gcc atg gcc      240
Asn Phe Leu Met Ser Ile Tyr Ser Phe Gly Ser Phe Leu Ala Met Ala         
65                  70                  75                  80          

tat gcc cta tca gta act ggc atc ctc tcc ggc gac tgt gag acg gcg      288
Tyr Ala Leu Ser Val Thr Gly Ile Leu Ser Gly Asp Cys Glu Thr Ala         
                85                  90                  95              

ttc aac aac gat gtg ttc agg atc aca act cag ctg ttc tac ctc agc      336
Phe Asn Asn Asp Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser         
            100                 105                 110                 

aag ttc gta gag tac atc gac tcc ttc tac ctt ccc ctt atg gac aag      384
Lys Phe Val Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Asp Lys         
        115                 120                 125                     

cca ctg tcg ttc ctt cag ttc ttc cat cat ttg ggg gcc ccc att gac      432
Pro Leu Ser Phe Leu Gln Phe Phe His His Leu Gly Ala Pro Ile Asp         
    130                 135                 140                         

atg tgg cta ttc tac aaa tac cgc aac gaa gga gtc tgg atc ttt gtc      480
Met Trp Leu Phe Tyr Lys Tyr Arg Asn Glu Gly Val Trp Ile Phe Val         
145                 150                 155                 160         

ctg ttg aat ggg ttc att cac tgg atc atg tac ggt tac tat tgg acg      528
Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr         
                165                 170                 175             

cgg ctc atc aag ctg aac ttc ccc atg ccc aag aac ctg atc acc tcc      576
Arg Leu Ile Lys Leu Asn Phe Pro Met Pro Lys Asn Leu Ile Thr Ser         
            180                 185                 190                 

atg cag atc atc cag ttc aat gtc ggg ttc tac atc gtc tgg aag tac      624
Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr         
        195                 200                 205                     

cgc aat gtg cca tgc tac cgc cag gat ggg atg cgc atg ttt gcc tgg      672
Arg Asn Val Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Ala Trp         
    210                 215                 220                         

atc ttc aac tac tgg tat gtc ggg acg gtc ttg ctg ctg ttc ctc aac      720
Ile Phe Asn Tyr Trp Tyr Val Gly Thr Val Leu Leu Leu Phe Leu Asn         
225                 230                 235                 240         

ttt tac gtg cag acg tac atc cgg aag ccg agg aag aac cga ggg aag      768
Phe Tyr Val Gln Thr Tyr Ile Arg Lys Pro Arg Lys Asn Arg Gly Lys         
                245                 250                 255             

aag gag                                                              774
Lys Glu                                                                 
                                                                        


<210>  13
<211>  258
<212>  PRT
<213>  Euglena anabaena UTEX 373

<400>  13

Met Glu Ala Ala Lys Glu Leu Val Ser Ile Val Gln Glu Glu Leu Pro 
1               5                   10                  15      


Lys Val Asp Tyr Ala Gln Leu Trp Gln Asp Ala Ser Ser Cys Glu Val 
            20                  25                  30          


Leu Tyr Leu Ser Val Ala Phe Val Ala Ile Lys Phe Met Leu Arg Pro 
        35                  40                  45              


Leu Asp Leu Lys Arg Gln Ala Thr Leu Lys Lys Leu Phe Thr Ala Tyr 
    50                  55                  60                  


Asn Phe Leu Met Ser Ile Tyr Ser Phe Gly Ser Phe Leu Ala Met Ala 
65                  70                  75                  80  


Tyr Ala Leu Ser Val Thr Gly Ile Leu Ser Gly Asp Cys Glu Thr Ala 
                85                  90                  95      


Phe Asn Asn Asp Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser 
            100                 105                 110         


Lys Phe Val Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Asp Lys 
        115                 120                 125             


Pro Leu Ser Phe Leu Gln Phe Phe His His Leu Gly Ala Pro Ile Asp 
    130                 135                 140                 


Met Trp Leu Phe Tyr Lys Tyr Arg Asn Glu Gly Val Trp Ile Phe Val 
145                 150                 155                 160 


Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr 
                165                 170                 175     


Arg Leu Ile Lys Leu Asn Phe Pro Met Pro Lys Asn Leu Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 
        195                 200                 205             


Arg Asn Val Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Ala Trp 
    210                 215                 220                 


Ile Phe Asn Tyr Trp Tyr Val Gly Thr Val Leu Leu Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Val Gln Thr Tyr Ile Arg Lys Pro Arg Lys Asn Arg Gly Lys 
                245                 250                 255     


Lys Glu 
        


<210>  14
<211>  774
<212>  DNA
<213>  Euglena anabaena UTEX 373


<220>
<221>  CDS
<222>  (1)..(774)
<223>  synthetic delta-9 elongase (codon-optimized for Yarrowia 
       lipolytica)

<400>  14
atg gag gct gcc aag gag ctg gtc tcc atc gtc cag gag gaa ctt ccc       48
Met Glu Ala Ala Lys Glu Leu Val Ser Ile Val Gln Glu Glu Leu Pro         
1               5                   10                  15              

aag gtg gac tac gcc cag ctc tgg cag gac gcc tcc tct tgc gag gtt       96
Lys Val Asp Tyr Ala Gln Leu Trp Gln Asp Ala Ser Ser Cys Glu Val         
            20                  25                  30                  

ctg tac ctc tcg gtc gct ttc gtg gcc atc aag ttc atg ctt cga cct      144
Leu Tyr Leu Ser Val Ala Phe Val Ala Ile Lys Phe Met Leu Arg Pro         
        35                  40                  45                      

ctg gac ctc aag cga caa gcc acc ctc aaa aag ctg ttc acc gca tac      192
Leu Asp Leu Lys Arg Gln Ala Thr Leu Lys Lys Leu Phe Thr Ala Tyr         
    50                  55                  60                          

aac ttt ctc atg tcc atc tac tcg ttc ggc tcc ttc ctg gcg atg gcc      240
Asn Phe Leu Met Ser Ile Tyr Ser Phe Gly Ser Phe Leu Ala Met Ala         
65                  70                  75                  80          

tac gct ctc tct gtc act ggt att ctt tcc ggc gat tgt gag act gcc      288
Tyr Ala Leu Ser Val Thr Gly Ile Leu Ser Gly Asp Cys Glu Thr Ala         
                85                  90                  95              

ttc aac aat gac gtg ttc cga atc acc act cag ctg ttc tac ctc agc      336
Phe Asn Asn Asp Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser         
            100                 105                 110                 

aag ttc gtc gag tac atc gac tcc ttc tac ctt ccc ctc atg gac aag      384
Lys Phe Val Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Asp Lys         
        115                 120                 125                     

ccc ttg tcg ttt ctg cag ttc ttt cac cat ctc gga gct ccc atc gac      432
Pro Leu Ser Phe Leu Gln Phe Phe His His Leu Gly Ala Pro Ile Asp         
    130                 135                 140                         

atg tgg ctg ttc tac aag tat cga aac gaa ggc gtc tgg atc ttt gtt      480
Met Trp Leu Phe Tyr Lys Tyr Arg Asn Glu Gly Val Trp Ile Phe Val         
145                 150                 155                 160         

ctg ctc aac ggc ttc att cac tgg atc atg tac ggt tac tat tgg acg      528
Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr         
                165                 170                 175             

cga ctc atc aag ctg aac ttc cct atg ccc aag aac ctc att acc tcc      576
Arg Leu Ile Lys Leu Asn Phe Pro Met Pro Lys Asn Leu Ile Thr Ser         
            180                 185                 190                 

atg caa att atc cag ttc aac gtc gga ttc tac atc gtc tgg aag tac      624
Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr         
        195                 200                 205                     

cga aac gtg ccc tgc tac cgg cag gac ggt atg cga atg ttt gcc tgg      672
Arg Asn Val Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Ala Trp         
    210                 215                 220                         

atc ttc aac tac tgg tat gtc ggc acg gtg ctg ctt ctg ttc ctc aac      720
Ile Phe Asn Tyr Trp Tyr Val Gly Thr Val Leu Leu Leu Phe Leu Asn         
225                 230                 235                 240         

ttc tac gtc cag acc tac att cgg aag cct cga aag aac cga ggc aaa      768
Phe Tyr Val Gln Thr Tyr Ile Arg Lys Pro Arg Lys Asn Arg Gly Lys         
                245                 250                 255             

aag gag                                                              774
Lys Glu                                                                 
                                                                        


<210>  15
<211>  258
<212>  PRT
<213>  Euglena anabaena UTEX 373

<400>  15

Met Glu Ala Ala Lys Glu Leu Val Ser Ile Val Gln Glu Glu Leu Pro 
1               5                   10                  15      


Lys Val Asp Tyr Ala Gln Leu Trp Gln Asp Ala Ser Ser Cys Glu Val 
            20                  25                  30          


Leu Tyr Leu Ser Val Ala Phe Val Ala Ile Lys Phe Met Leu Arg Pro 
        35                  40                  45              


Leu Asp Leu Lys Arg Gln Ala Thr Leu Lys Lys Leu Phe Thr Ala Tyr 
    50                  55                  60                  


Asn Phe Leu Met Ser Ile Tyr Ser Phe Gly Ser Phe Leu Ala Met Ala 
65                  70                  75                  80  


Tyr Ala Leu Ser Val Thr Gly Ile Leu Ser Gly Asp Cys Glu Thr Ala 
                85                  90                  95      


Phe Asn Asn Asp Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser 
            100                 105                 110         


Lys Phe Val Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Asp Lys 
        115                 120                 125             


Pro Leu Ser Phe Leu Gln Phe Phe His His Leu Gly Ala Pro Ile Asp 
    130                 135                 140                 


Met Trp Leu Phe Tyr Lys Tyr Arg Asn Glu Gly Val Trp Ile Phe Val 
145                 150                 155                 160 


Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr 
                165                 170                 175     


Arg Leu Ile Lys Leu Asn Phe Pro Met Pro Lys Asn Leu Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 
        195                 200                 205             


Arg Asn Val Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Ala Trp 
    210                 215                 220                 


Ile Phe Asn Tyr Trp Tyr Val Gly Thr Val Leu Leu Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Val Gln Thr Tyr Ile Arg Lys Pro Arg Lys Asn Arg Gly Lys 
                245                 250                 255     


Lys Glu 
        


<210>  16
<211>  774
<212>  DNA
<213>  Euglena anabaena UTEX 373


<220>
<221>  CDS
<222>  (1)..(774)
<223>  delta-9 elongase

<400>  16
atg gaa gca gcc aaa gaa ttg gtt tcc atc gtc caa gag gag ctc ccc       48
Met Glu Ala Ala Lys Glu Leu Val Ser Ile Val Gln Glu Glu Leu Pro         
1               5                   10                  15              

aag gtg gac tat gcc cag ctt tgg cag gac gcc agc agc tgt gag gtc       96
Lys Val Asp Tyr Ala Gln Leu Trp Gln Asp Ala Ser Ser Cys Glu Val         
            20                  25                  30                  

ctt tac ctc tcg gtg gca ttc gtg gcg atc aag ttc atg ctg cgc cca      144
Leu Tyr Leu Ser Val Ala Phe Val Ala Ile Lys Phe Met Leu Arg Pro         
        35                  40                  45                      

ctg gac ctg aag cgc cag gcc acc ttg aag aag ctg ttc aca gca tac      192
Leu Asp Leu Lys Arg Gln Ala Thr Leu Lys Lys Leu Phe Thr Ala Tyr         
    50                  55                  60                          

aac ttc ctc atg tcg atc tat tcc ttt ggc tcc ttc ctg gcc atg gcc      240
Asn Phe Leu Met Ser Ile Tyr Ser Phe Gly Ser Phe Leu Ala Met Ala         
65                  70                  75                  80          

tat gcc cta tca gta act ggc atc ctc tcc ggc gac tgt gag aca gcg      288
Tyr Ala Leu Ser Val Thr Gly Ile Leu Ser Gly Asp Cys Glu Thr Ala         
                85                  90                  95              

ttc aac aac gat gtg ttc agg atc aca act cag ctg ttc tac ctc agc      336
Phe Asn Asn Asp Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser         
            100                 105                 110                 

aag ttc gta gag tac atc gac tcc ttc tac ctt ccc ctt atg gac aag      384
Lys Phe Val Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Asp Lys         
        115                 120                 125                     

cca ctg tcg ttc ctt cag ttc ttc cat cat ttg ggg gct ccc att gac      432
Pro Leu Ser Phe Leu Gln Phe Phe His His Leu Gly Ala Pro Ile Asp         
    130                 135                 140                         

atg tgg cta ttc tac aaa tac cgc aac gaa gga gtc tgg atc ttt gtc      480
Met Trp Leu Phe Tyr Lys Tyr Arg Asn Glu Gly Val Trp Ile Phe Val         
145                 150                 155                 160         

ctg ttg aat ggg ttc att cac tgg atc atg tac ggt tac tac tgg acg      528
Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr         
                165                 170                 175             

cgg ctc atc aag ctg aac ttc ccc atg ccc aag aac ctg atc acc tcc      576
Arg Leu Ile Lys Leu Asn Phe Pro Met Pro Lys Asn Leu Ile Thr Ser         
            180                 185                 190                 

atg cag atc atc cag ttc aat gtc ggg ttc tac atc gtc tgg aag tac      624
Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr         
        195                 200                 205                     

cgc aat gtg cca tgc tac cgc cag gat ggg atg cgc atg ttt gcc tgg      672
Arg Asn Val Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Ala Trp         
    210                 215                 220                         

atc ttc aac tac tgg tac gtc ggg acg gtc ttg ctg ctg ttc ctc aac      720
Ile Phe Asn Tyr Trp Tyr Val Gly Thr Val Leu Leu Leu Phe Leu Asn         
225                 230                 235                 240         

ttt tac gtg cag acg tac atc cgg aag ccg agg aag aac caa ggg aag      768
Phe Tyr Val Gln Thr Tyr Ile Arg Lys Pro Arg Lys Asn Gln Gly Lys         
                245                 250                 255             

aag gag                                                              774
Lys Glu                                                                 
                                                                        


<210>  17
<211>  258
<212>  PRT
<213>  Euglena anabaena UTEX 373

<400>  17

Met Glu Ala Ala Lys Glu Leu Val Ser Ile Val Gln Glu Glu Leu Pro 
1               5                   10                  15      


Lys Val Asp Tyr Ala Gln Leu Trp Gln Asp Ala Ser Ser Cys Glu Val 
            20                  25                  30          


Leu Tyr Leu Ser Val Ala Phe Val Ala Ile Lys Phe Met Leu Arg Pro 
        35                  40                  45              


Leu Asp Leu Lys Arg Gln Ala Thr Leu Lys Lys Leu Phe Thr Ala Tyr 
    50                  55                  60                  


Asn Phe Leu Met Ser Ile Tyr Ser Phe Gly Ser Phe Leu Ala Met Ala 
65                  70                  75                  80  


Tyr Ala Leu Ser Val Thr Gly Ile Leu Ser Gly Asp Cys Glu Thr Ala 
                85                  90                  95      


Phe Asn Asn Asp Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser 
            100                 105                 110         


Lys Phe Val Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Asp Lys 
        115                 120                 125             


Pro Leu Ser Phe Leu Gln Phe Phe His His Leu Gly Ala Pro Ile Asp 
    130                 135                 140                 


Met Trp Leu Phe Tyr Lys Tyr Arg Asn Glu Gly Val Trp Ile Phe Val 
145                 150                 155                 160 


Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr 
                165                 170                 175     


Arg Leu Ile Lys Leu Asn Phe Pro Met Pro Lys Asn Leu Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 
        195                 200                 205             


Arg Asn Val Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Ala Trp 
    210                 215                 220                 


Ile Phe Asn Tyr Trp Tyr Val Gly Thr Val Leu Leu Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Val Gln Thr Tyr Ile Arg Lys Pro Arg Lys Asn Gln Gly Lys 
                245                 250                 255     


Lys Glu 
        


<210>  18
<211>  1271
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (4)..(1269)
<223>  delta-8 desaturase ("Eg5" or "EgD8")

<300>
<302>  DELTA-8 DESATURASE AND ITS USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  WO 2006/012325 and WO 2006/012326
<311>  2005-06-24
<312>  2006-02-02
<313>  (1)..(1271)

<300>
<302>  DELTA-8 DESATURASE AND ITS USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  U.S. 7,256,033 
<311>  2005-06-24
<312>  2007-08-14
<313>  (1)..(1271)

<400>  18
gaa atg aag tca aag cgc caa gcg ctt ccc ctt aca att gat gga aca       48
    Met Lys Ser Lys Arg Gln Ala Leu Pro Leu Thr Ile Asp Gly Thr         
    1               5                   10                  15          

aca tat gat gtg tct gcc tgg gtc aat ttc cac cct ggt ggt gcg gaa       96
Thr Tyr Asp Val Ser Ala Trp Val Asn Phe His Pro Gly Gly Ala Glu         
                20                  25                  30              

att ata gag aat tac caa gga agg gat gcc act gat gcc ttc atg gtt      144
Ile Ile Glu Asn Tyr Gln Gly Arg Asp Ala Thr Asp Ala Phe Met Val         
            35                  40                  45                  

atg cac tct caa gaa gcc ttc gac aag ctc aag cgc atg ccc aaa atc      192
Met His Ser Gln Glu Ala Phe Asp Lys Leu Lys Arg Met Pro Lys Ile         
        50                  55                  60                      

aat ccc agt tct gag ttg cca ccc cag gct gca gtg aat gaa gct caa      240
Asn Pro Ser Ser Glu Leu Pro Pro Gln Ala Ala Val Asn Glu Ala Gln         
    65                  70                  75                          

gag gat ttc cgg aag ctc cga gaa gag ttg atc gca act ggc atg ttt      288
Glu Asp Phe Arg Lys Leu Arg Glu Glu Leu Ile Ala Thr Gly Met Phe         
80                  85                  90                  95          

gat gcc tcc ccc ctc tgg tac tca tac aaa atc agc acc aca ctg ggc      336
Asp Ala Ser Pro Leu Trp Tyr Ser Tyr Lys Ile Ser Thr Thr Leu Gly         
                100                 105                 110             

ctt gga gtg ctg ggt tat ttc ctg atg gtt cag tat cag atg tat ttc      384
Leu Gly Val Leu Gly Tyr Phe Leu Met Val Gln Tyr Gln Met Tyr Phe         
            115                 120                 125                 

att ggg gca gtg ttg ctt ggg atg cac tat caa cag atg ggc tgg ctt      432
Ile Gly Ala Val Leu Leu Gly Met His Tyr Gln Gln Met Gly Trp Leu         
        130                 135                 140                     

tct cat gac att tgc cac cac cag act ttc aag aac cgg aac tgg aac      480
Ser His Asp Ile Cys His His Gln Thr Phe Lys Asn Arg Asn Trp Asn         
    145                 150                 155                         

aac ctc gtg gga ctg gta ttt ggc aat ggt ctg caa ggt ttt tcc gtg      528
Asn Leu Val Gly Leu Val Phe Gly Asn Gly Leu Gln Gly Phe Ser Val         
160                 165                 170                 175         

aca tgg tgg aag gac aga cac aat gca cat cat tcg gca acc aat gtt      576
Thr Trp Trp Lys Asp Arg His Asn Ala His His Ser Ala Thr Asn Val         
                180                 185                 190             

caa ggg cac gac cct gat att gac aac ctc ccc ctc tta gcc tgg tct      624
Gln Gly His Asp Pro Asp Ile Asp Asn Leu Pro Leu Leu Ala Trp Ser         
            195                 200                 205                 

gag gat gac gtc aca cgg gcg tca ccg att tcc cgc aag ctc att cag      672
Glu Asp Asp Val Thr Arg Ala Ser Pro Ile Ser Arg Lys Leu Ile Gln         
        210                 215                 220                     

ttc cag cag tac tat ttc ttg gtc atc tgt atc ttg ttg cgg ttc att      720
Phe Gln Gln Tyr Tyr Phe Leu Val Ile Cys Ile Leu Leu Arg Phe Ile         
    225                 230                 235                         

tgg tgt ttc cag agc gtg ttg acc gtg cgc agt ttg aag gac aga gat      768
Trp Cys Phe Gln Ser Val Leu Thr Val Arg Ser Leu Lys Asp Arg Asp         
240                 245                 250                 255         

aac caa ttc tat cgc tct cag tat aag aag gag gcc att ggc ctc gcc      816
Asn Gln Phe Tyr Arg Ser Gln Tyr Lys Lys Glu Ala Ile Gly Leu Ala         
                260                 265                 270             

ctg cac tgg acc ttg aag acc ctg ttc cac tta ttc ttt atg ccc agc      864
Leu His Trp Thr Leu Lys Thr Leu Phe His Leu Phe Phe Met Pro Ser         
            275                 280                 285                 

atc ctc aca tcg ctg ttg gtg ttt ttc gtt tcg gag ctg gtt ggc ggc      912
Ile Leu Thr Ser Leu Leu Val Phe Phe Val Ser Glu Leu Val Gly Gly         
        290                 295                 300                     

ttc ggc att gcg atc gtg gtg ttc atg aac cac tac cca ctg gag aag      960
Phe Gly Ile Ala Ile Val Val Phe Met Asn His Tyr Pro Leu Glu Lys         
    305                 310                 315                         

atc ggg gac tca gtc tgg gat ggc cat gga ttc tcg gtt ggc cag atc     1008
Ile Gly Asp Ser Val Trp Asp Gly His Gly Phe Ser Val Gly Gln Ile         
320                 325                 330                 335         

cat gag acc atg aac att cgg cga ggg att atc aca gat tgg ttt ttc     1056
His Glu Thr Met Asn Ile Arg Arg Gly Ile Ile Thr Asp Trp Phe Phe         
                340                 345                 350             

gga ggc ttg aat tac cag att gag cac cat ttg tgg ccg acc ctc cct     1104
Gly Gly Leu Asn Tyr Gln Ile Glu His His Leu Trp Pro Thr Leu Pro         
            355                 360                 365                 

cgc cac aac ctg aca gcg gtt agc tac cag gtg gaa cag ctg tgc cag     1152
Arg His Asn Leu Thr Ala Val Ser Tyr Gln Val Glu Gln Leu Cys Gln         
        370                 375                 380                     

aag cac aac ctg ccg tat cgg aac ccg ctg ccc cat gaa ggg ttg gtc     1200
Lys His Asn Leu Pro Tyr Arg Asn Pro Leu Pro His Glu Gly Leu Val         
    385                 390                 395                         

atc ctg ctg cgc tat ctg gcg gtg ttc gcc cgg atg gcg gag aag caa     1248
Ile Leu Leu Arg Tyr Leu Ala Val Phe Ala Arg Met Ala Glu Lys Gln         
400                 405                 410                 415         

ccc gcg ggg aag gct cta taa gg                                      1271
Pro Ala Gly Lys Ala Leu                                                 
                420                                                     


<210>  19
<211>  421
<212>  PRT
<213>  Euglena gracilis

<400>  19

Met Lys Ser Lys Arg Gln Ala Leu Pro Leu Thr Ile Asp Gly Thr Thr 
1               5                   10                  15      


Tyr Asp Val Ser Ala Trp Val Asn Phe His Pro Gly Gly Ala Glu Ile 
            20                  25                  30          


Ile Glu Asn Tyr Gln Gly Arg Asp Ala Thr Asp Ala Phe Met Val Met 
        35                  40                  45              


His Ser Gln Glu Ala Phe Asp Lys Leu Lys Arg Met Pro Lys Ile Asn 
    50                  55                  60                  


Pro Ser Ser Glu Leu Pro Pro Gln Ala Ala Val Asn Glu Ala Gln Glu 
65                  70                  75                  80  


Asp Phe Arg Lys Leu Arg Glu Glu Leu Ile Ala Thr Gly Met Phe Asp 
                85                  90                  95      


Ala Ser Pro Leu Trp Tyr Ser Tyr Lys Ile Ser Thr Thr Leu Gly Leu 
            100                 105                 110         


Gly Val Leu Gly Tyr Phe Leu Met Val Gln Tyr Gln Met Tyr Phe Ile 
        115                 120                 125             


Gly Ala Val Leu Leu Gly Met His Tyr Gln Gln Met Gly Trp Leu Ser 
    130                 135                 140                 


His Asp Ile Cys His His Gln Thr Phe Lys Asn Arg Asn Trp Asn Asn 
145                 150                 155                 160 


Leu Val Gly Leu Val Phe Gly Asn Gly Leu Gln Gly Phe Ser Val Thr 
                165                 170                 175     


Trp Trp Lys Asp Arg His Asn Ala His His Ser Ala Thr Asn Val Gln 
            180                 185                 190         


Gly His Asp Pro Asp Ile Asp Asn Leu Pro Leu Leu Ala Trp Ser Glu 
        195                 200                 205             


Asp Asp Val Thr Arg Ala Ser Pro Ile Ser Arg Lys Leu Ile Gln Phe 
    210                 215                 220                 


Gln Gln Tyr Tyr Phe Leu Val Ile Cys Ile Leu Leu Arg Phe Ile Trp 
225                 230                 235                 240 


Cys Phe Gln Ser Val Leu Thr Val Arg Ser Leu Lys Asp Arg Asp Asn 
                245                 250                 255     


Gln Phe Tyr Arg Ser Gln Tyr Lys Lys Glu Ala Ile Gly Leu Ala Leu 
            260                 265                 270         


His Trp Thr Leu Lys Thr Leu Phe His Leu Phe Phe Met Pro Ser Ile 
        275                 280                 285             


Leu Thr Ser Leu Leu Val Phe Phe Val Ser Glu Leu Val Gly Gly Phe 
    290                 295                 300                 


Gly Ile Ala Ile Val Val Phe Met Asn His Tyr Pro Leu Glu Lys Ile 
305                 310                 315                 320 


Gly Asp Ser Val Trp Asp Gly His Gly Phe Ser Val Gly Gln Ile His 
                325                 330                 335     


Glu Thr Met Asn Ile Arg Arg Gly Ile Ile Thr Asp Trp Phe Phe Gly 
            340                 345                 350         


Gly Leu Asn Tyr Gln Ile Glu His His Leu Trp Pro Thr Leu Pro Arg 
        355                 360                 365             


His Asn Leu Thr Ala Val Ser Tyr Gln Val Glu Gln Leu Cys Gln Lys 
    370                 375                 380                 


His Asn Leu Pro Tyr Arg Asn Pro Leu Pro His Glu Gly Leu Val Ile 
385                 390                 395                 400 


Leu Leu Arg Tyr Leu Ala Val Phe Ala Arg Met Ala Glu Lys Gln Pro 
                405                 410                 415     


Ala Gly Lys Ala Leu 
            420     


<210>  20
<211>  1272
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (2)..(1270)
<223>  synthetic delta-8 desaturase (codon-optimized for Yarrowia 
       lipolytica) ("D8SF" or "EgD8S")

<300>
<302>  DELTA-8 DESATURASE AND ITS USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  WO 2006/012325 and WO 2006/012326
<311>  2005-06-24
<312>  2006-02-02
<313>  (1)..(1272)

<300>
<302>  DELTA-8 DESATURASE AND ITS USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  U.S. 7,256,033 
<311>  2005-06-24
<312>  2007-08-14
<313>  (1)..(1272)

<400>  20
c atg gtg aag tcc aag cga cag gct ctg ccc ctc acc atc gac gga act     49
  Met Val Lys Ser Lys Arg Gln Ala Leu Pro Leu Thr Ile Asp Gly Thr       
  1               5                   10                  15            

acc tac gac gtc tcc gct tgg gtg aac ttc cac cct ggt gga gct gaa       97
Thr Tyr Asp Val Ser Ala Trp Val Asn Phe His Pro Gly Gly Ala Glu         
            20                  25                  30                  

atc att gag aac tac cag gga cga gat gct act gac gcc ttc atg gtt      145
Ile Ile Glu Asn Tyr Gln Gly Arg Asp Ala Thr Asp Ala Phe Met Val         
        35                  40                  45                      

atg cac tct cag gaa gcc ttc gac aag ctc aag cga atg ccc aag atc      193
Met His Ser Gln Glu Ala Phe Asp Lys Leu Lys Arg Met Pro Lys Ile         
    50                  55                  60                          

aac ccc tcc tcc gag ctg cct ccc cag gct gcc gtc aac gaa gct cag      241
Asn Pro Ser Ser Glu Leu Pro Pro Gln Ala Ala Val Asn Glu Ala Gln         
65                  70                  75                  80          

gag gat ttc cga aag ctc cga gaa gag ctg atc gcc act ggc atg ttt      289
Glu Asp Phe Arg Lys Leu Arg Glu Glu Leu Ile Ala Thr Gly Met Phe         
                85                  90                  95              

gac gcc tct ccc ctc tgg tac tcg tac aag atc tcc acc acc ctg ggt      337
Asp Ala Ser Pro Leu Trp Tyr Ser Tyr Lys Ile Ser Thr Thr Leu Gly         
            100                 105                 110                 

ctt ggc gtg ctt gga tac ttc ctg atg gtc cag tac cag atg tac ttc      385
Leu Gly Val Leu Gly Tyr Phe Leu Met Val Gln Tyr Gln Met Tyr Phe         
        115                 120                 125                     

att ggt gct gtg ctg ctc ggt atg cac tac cag caa atg gga tgg ctg      433
Ile Gly Ala Val Leu Leu Gly Met His Tyr Gln Gln Met Gly Trp Leu         
    130                 135                 140                         

tct cat gac atc tgc cac cac cag acc ttc aag aac cga aac tgg aat      481
Ser His Asp Ile Cys His His Gln Thr Phe Lys Asn Arg Asn Trp Asn         
145                 150                 155                 160         

aac ctc gtg ggt ctg gtc ttt ggc aac gga ctc cag ggc ttc tcc gtg      529
Asn Leu Val Gly Leu Val Phe Gly Asn Gly Leu Gln Gly Phe Ser Val         
                165                 170                 175             

acc tgg tgg aag gac aga cac aac gcc cat cat tct gct acc aac gtt      577
Thr Trp Trp Lys Asp Arg His Asn Ala His His Ser Ala Thr Asn Val         
            180                 185                 190                 

cag ggt cac gat ccc gac att gat aac ctg cct ctg ctc gcc tgg tcc      625
Gln Gly His Asp Pro Asp Ile Asp Asn Leu Pro Leu Leu Ala Trp Ser         
        195                 200                 205                     

gag gac gat gtc act cga gct tct ccc atc tcc cga aag ctc att cag      673
Glu Asp Asp Val Thr Arg Ala Ser Pro Ile Ser Arg Lys Leu Ile Gln         
    210                 215                 220                         

ttc caa cag tac tat ttc ctg gtc atc tgt att ctc ctg cga ttc atc      721
Phe Gln Gln Tyr Tyr Phe Leu Val Ile Cys Ile Leu Leu Arg Phe Ile         
225                 230                 235                 240         

tgg tgt ttc cag tct gtg ctg acc gtt cga tcc ctc aag gac cga gac      769
Trp Cys Phe Gln Ser Val Leu Thr Val Arg Ser Leu Lys Asp Arg Asp         
                245                 250                 255             

aac cag ttc tac cga tct cag tac aag aaa gag gcc att gga ctc gct      817
Asn Gln Phe Tyr Arg Ser Gln Tyr Lys Lys Glu Ala Ile Gly Leu Ala         
            260                 265                 270                 

ctg cac tgg act ctc aag acc ctg ttc cac ctc ttc ttt atg ccc tcc      865
Leu His Trp Thr Leu Lys Thr Leu Phe His Leu Phe Phe Met Pro Ser         
        275                 280                 285                     

atc ctg acc tcg ctc ctg gtg ttc ttt gtt tcc gag ctc gtc ggt ggc      913
Ile Leu Thr Ser Leu Leu Val Phe Phe Val Ser Glu Leu Val Gly Gly         
    290                 295                 300                         

ttc gga att gcc atc gtg gtc ttc atg aac cac tac cct ctg gag aag      961
Phe Gly Ile Ala Ile Val Val Phe Met Asn His Tyr Pro Leu Glu Lys         
305                 310                 315                 320         

atc ggt gat tcc gtc tgg gac gga cat ggc ttc tct gtg ggt cag atc     1009
Ile Gly Asp Ser Val Trp Asp Gly His Gly Phe Ser Val Gly Gln Ile         
                325                 330                 335             

cat gag acc atg aac att cga cga ggc atc att act gac tgg ttc ttt     1057
His Glu Thr Met Asn Ile Arg Arg Gly Ile Ile Thr Asp Trp Phe Phe         
            340                 345                 350                 

gga ggc ctg aac tac cag atc gag cac cat ctc tgg ccc acc ctg cct     1105
Gly Gly Leu Asn Tyr Gln Ile Glu His His Leu Trp Pro Thr Leu Pro         
        355                 360                 365                     

cga cac aac ctc act gcc gtt tcc tac cag gtg gaa cag ctg tgc cag     1153
Arg His Asn Leu Thr Ala Val Ser Tyr Gln Val Glu Gln Leu Cys Gln         
    370                 375                 380                         

aag cac aac ctc ccc tac cga aac cct ctg ccc cat gaa ggt ctc gtc     1201
Lys His Asn Leu Pro Tyr Arg Asn Pro Leu Pro His Glu Gly Leu Val         
385                 390                 395                 400         

atc ctg ctc cga tac ctg gcc gtg ttc gct cga atg gcc gag aag cag     1249
Ile Leu Leu Arg Tyr Leu Ala Val Phe Ala Arg Met Ala Glu Lys Gln         
                405                 410                 415             

ccc gct ggc aag gct ctc taa gc                                      1272
Pro Ala Gly Lys Ala Leu                                                 
            420                                                         


<210>  21
<211>  422
<212>  PRT
<213>  Euglena gracilis

<400>  21

Met Val Lys Ser Lys Arg Gln Ala Leu Pro Leu Thr Ile Asp Gly Thr 
1               5                   10                  15      


Thr Tyr Asp Val Ser Ala Trp Val Asn Phe His Pro Gly Gly Ala Glu 
            20                  25                  30          


Ile Ile Glu Asn Tyr Gln Gly Arg Asp Ala Thr Asp Ala Phe Met Val 
        35                  40                  45              


Met His Ser Gln Glu Ala Phe Asp Lys Leu Lys Arg Met Pro Lys Ile 
    50                  55                  60                  


Asn Pro Ser Ser Glu Leu Pro Pro Gln Ala Ala Val Asn Glu Ala Gln 
65                  70                  75                  80  


Glu Asp Phe Arg Lys Leu Arg Glu Glu Leu Ile Ala Thr Gly Met Phe 
                85                  90                  95      


Asp Ala Ser Pro Leu Trp Tyr Ser Tyr Lys Ile Ser Thr Thr Leu Gly 
            100                 105                 110         


Leu Gly Val Leu Gly Tyr Phe Leu Met Val Gln Tyr Gln Met Tyr Phe 
        115                 120                 125             


Ile Gly Ala Val Leu Leu Gly Met His Tyr Gln Gln Met Gly Trp Leu 
    130                 135                 140                 


Ser His Asp Ile Cys His His Gln Thr Phe Lys Asn Arg Asn Trp Asn 
145                 150                 155                 160 


Asn Leu Val Gly Leu Val Phe Gly Asn Gly Leu Gln Gly Phe Ser Val 
                165                 170                 175     


Thr Trp Trp Lys Asp Arg His Asn Ala His His Ser Ala Thr Asn Val 
            180                 185                 190         


Gln Gly His Asp Pro Asp Ile Asp Asn Leu Pro Leu Leu Ala Trp Ser 
        195                 200                 205             


Glu Asp Asp Val Thr Arg Ala Ser Pro Ile Ser Arg Lys Leu Ile Gln 
    210                 215                 220                 


Phe Gln Gln Tyr Tyr Phe Leu Val Ile Cys Ile Leu Leu Arg Phe Ile 
225                 230                 235                 240 


Trp Cys Phe Gln Ser Val Leu Thr Val Arg Ser Leu Lys Asp Arg Asp 
                245                 250                 255     


Asn Gln Phe Tyr Arg Ser Gln Tyr Lys Lys Glu Ala Ile Gly Leu Ala 
            260                 265                 270         


Leu His Trp Thr Leu Lys Thr Leu Phe His Leu Phe Phe Met Pro Ser 
        275                 280                 285             


Ile Leu Thr Ser Leu Leu Val Phe Phe Val Ser Glu Leu Val Gly Gly 
    290                 295                 300                 


Phe Gly Ile Ala Ile Val Val Phe Met Asn His Tyr Pro Leu Glu Lys 
305                 310                 315                 320 


Ile Gly Asp Ser Val Trp Asp Gly His Gly Phe Ser Val Gly Gln Ile 
                325                 330                 335     


His Glu Thr Met Asn Ile Arg Arg Gly Ile Ile Thr Asp Trp Phe Phe 
            340                 345                 350         


Gly Gly Leu Asn Tyr Gln Ile Glu His His Leu Trp Pro Thr Leu Pro 
        355                 360                 365             


Arg His Asn Leu Thr Ala Val Ser Tyr Gln Val Glu Gln Leu Cys Gln 
    370                 375                 380                 


Lys His Asn Leu Pro Tyr Arg Asn Pro Leu Pro His Glu Gly Leu Val 
385                 390                 395                 400 


Ile Leu Leu Arg Tyr Leu Ala Val Phe Ala Arg Met Ala Glu Lys Gln 
                405                 410                 415     


Pro Ala Gly Lys Ala Leu 
            420         


<210>  22
<211>  1272
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutant EgD8S-23 delta-8 desaturase


<220>
<221>  CDS
<222>  (2)..(1270)

<300>
<302>  MUTANT DELTA-8 DESATURASE GENES ENGINEERED BY TARGETED MUTAGENSIS
        AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS
<310>  WO 2008/073271
<311>  2007-12-05
<312>  2008-06-19
<313>  (1)..(1272)

<300>
<302>  MUTANT DELTA-8 DESATURASE GENES ENGINEERED BY TARGETED MUTAGENSIS
        AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS
<310>  US 2008-0138868-A1
<311>  2006-12-07
<312>  2008-06-12
<313>  (1)..(1272)

<400>  22
c atg gtg aag gct tct cga cag gct ctg ccc ctc gtc atc gac gga aag     49
  Met Val Lys Ala Ser Arg Gln Ala Leu Pro Leu Val Ile Asp Gly Lys       
  1               5                   10                  15            

gtg tac gac gtc tcc gct tgg gtg aac ttc cac cct ggt gga gct gaa       97
Val Tyr Asp Val Ser Ala Trp Val Asn Phe His Pro Gly Gly Ala Glu         
            20                  25                  30                  

atc att gag aac tac cag gga cga gat gct act gac gcc ttc atg gtt      145
Ile Ile Glu Asn Tyr Gln Gly Arg Asp Ala Thr Asp Ala Phe Met Val         
        35                  40                  45                      

atg cac tct cag gaa gcc ttc gac aag ctc aag cga atg ccc aag atc      193
Met His Ser Gln Glu Ala Phe Asp Lys Leu Lys Arg Met Pro Lys Ile         
    50                  55                  60                          

aac cag gct tcc gag ctg cct ccc cag gct gcc gtc aac gaa gct cag      241
Asn Gln Ala Ser Glu Leu Pro Pro Gln Ala Ala Val Asn Glu Ala Gln         
65                  70                  75                  80          

gag gat ttc cga aag ctc cga gaa gag ctg atc gcc act ggc atg ttt      289
Glu Asp Phe Arg Lys Leu Arg Glu Glu Leu Ile Ala Thr Gly Met Phe         
                85                  90                  95              

gac gcc tct ccc ctc tgg tac tcg tac aag atc ttg acc acc ctg ggt      337
Asp Ala Ser Pro Leu Trp Tyr Ser Tyr Lys Ile Leu Thr Thr Leu Gly         
            100                 105                 110                 

ctt ggc gtg ctt gcc ttc ttc atg ctg gtc cag tac cac ctg tac ttc      385
Leu Gly Val Leu Ala Phe Phe Met Leu Val Gln Tyr His Leu Tyr Phe         
        115                 120                 125                     

att ggt gct ctc gtg ctc ggt atg cac tac cag caa atg gga tgg ctg      433
Ile Gly Ala Leu Val Leu Gly Met His Tyr Gln Gln Met Gly Trp Leu         
    130                 135                 140                         

tct cat gac atc tgc cac cac cag acc ttc aag aac cga aac tgg aat      481
Ser His Asp Ile Cys His His Gln Thr Phe Lys Asn Arg Asn Trp Asn         
145                 150                 155                 160         

aac gtc ctg ggt ctg gtc ttt ggc aac gga ctc cag ggc ttc tcc gtg      529
Asn Val Leu Gly Leu Val Phe Gly Asn Gly Leu Gln Gly Phe Ser Val         
                165                 170                 175             

acc tgg tgg aag gac aga cac aac gcc cat cat tct gct acc aac gtt      577
Thr Trp Trp Lys Asp Arg His Asn Ala His His Ser Ala Thr Asn Val         
            180                 185                 190                 

cag ggt cac gat ccc gac att gat aac ctg cct ctg ctc gcc tgg tcc      625
Gln Gly His Asp Pro Asp Ile Asp Asn Leu Pro Leu Leu Ala Trp Ser         
        195                 200                 205                     

gag gac gat gtc act cga gct tct ccc atc tcc cga aag ctc att cag      673
Glu Asp Asp Val Thr Arg Ala Ser Pro Ile Ser Arg Lys Leu Ile Gln         
    210                 215                 220                         

ttc caa cag tac tat ttc ctg gtc atc tgt att ctc ctg cga ttc atc      721
Phe Gln Gln Tyr Tyr Phe Leu Val Ile Cys Ile Leu Leu Arg Phe Ile         
225                 230                 235                 240         

tgg tgt ttc cag tct gtg ctg acc gtt cga tcc ctc aag gac cga gac      769
Trp Cys Phe Gln Ser Val Leu Thr Val Arg Ser Leu Lys Asp Arg Asp         
                245                 250                 255             

aac cag ttc tac cga tct cag tac aag aaa gag gcc att gga ctc gct      817
Asn Gln Phe Tyr Arg Ser Gln Tyr Lys Lys Glu Ala Ile Gly Leu Ala         
            260                 265                 270                 

ctg cac tgg act ctc aag acc ctg ttc cac ctc ttc ttt atg ccc tcc      865
Leu His Trp Thr Leu Lys Thr Leu Phe His Leu Phe Phe Met Pro Ser         
        275                 280                 285                     

atc ctg acc tcg atg ctg gtg ttc ttt gtt tcc gag ctc gtc ggt ggc      913
Ile Leu Thr Ser Met Leu Val Phe Phe Val Ser Glu Leu Val Gly Gly         
    290                 295                 300                         

ttc gga att gcc atc gtg gtc ttc atg aac cac tac cct ctg gag aag      961
Phe Gly Ile Ala Ile Val Val Phe Met Asn His Tyr Pro Leu Glu Lys         
305                 310                 315                 320         

atc ggt gat tcc gtc tgg gac gga cat ggc ttc tct gtg ggt cag atc     1009
Ile Gly Asp Ser Val Trp Asp Gly His Gly Phe Ser Val Gly Gln Ile         
                325                 330                 335             

cat gag acc atg aac att cga cga ggc atc att act gac tgg ttc ttt     1057
His Glu Thr Met Asn Ile Arg Arg Gly Ile Ile Thr Asp Trp Phe Phe         
            340                 345                 350                 

gga ggc ctg aac tac cag atc gag cac cat ctc tgg ccc acc ctg cct     1105
Gly Gly Leu Asn Tyr Gln Ile Glu His His Leu Trp Pro Thr Leu Pro         
        355                 360                 365                     

cga cac aac ctc act gcc gtt tcc tac cag gtg gaa cag ctg tgc cag     1153
Arg His Asn Leu Thr Ala Val Ser Tyr Gln Val Glu Gln Leu Cys Gln         
    370                 375                 380                         

aag cac aac ctc ccc tac cga aac cct ctg ccc cat gaa ggt ctc gtc     1201
Lys His Asn Leu Pro Tyr Arg Asn Pro Leu Pro His Glu Gly Leu Val         
385                 390                 395                 400         

atc ctg ctc cga tac ctg tcc cag ttc gct cga atg gcc gag aag cag     1249
Ile Leu Leu Arg Tyr Leu Ser Gln Phe Ala Arg Met Ala Glu Lys Gln         
                405                 410                 415             

ccc ggt gcc aag gct cag taa gc                                      1272
Pro Gly Ala Lys Ala Gln                                                 
            420                                                         


<210>  23
<211>  422
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  23

Met Val Lys Ala Ser Arg Gln Ala Leu Pro Leu Val Ile Asp Gly Lys 
1               5                   10                  15      


Val Tyr Asp Val Ser Ala Trp Val Asn Phe His Pro Gly Gly Ala Glu 
            20                  25                  30          


Ile Ile Glu Asn Tyr Gln Gly Arg Asp Ala Thr Asp Ala Phe Met Val 
        35                  40                  45              


Met His Ser Gln Glu Ala Phe Asp Lys Leu Lys Arg Met Pro Lys Ile 
    50                  55                  60                  


Asn Gln Ala Ser Glu Leu Pro Pro Gln Ala Ala Val Asn Glu Ala Gln 
65                  70                  75                  80  


Glu Asp Phe Arg Lys Leu Arg Glu Glu Leu Ile Ala Thr Gly Met Phe 
                85                  90                  95      


Asp Ala Ser Pro Leu Trp Tyr Ser Tyr Lys Ile Leu Thr Thr Leu Gly 
            100                 105                 110         


Leu Gly Val Leu Ala Phe Phe Met Leu Val Gln Tyr His Leu Tyr Phe 
        115                 120                 125             


Ile Gly Ala Leu Val Leu Gly Met His Tyr Gln Gln Met Gly Trp Leu 
    130                 135                 140                 


Ser His Asp Ile Cys His His Gln Thr Phe Lys Asn Arg Asn Trp Asn 
145                 150                 155                 160 


Asn Val Leu Gly Leu Val Phe Gly Asn Gly Leu Gln Gly Phe Ser Val 
                165                 170                 175     


Thr Trp Trp Lys Asp Arg His Asn Ala His His Ser Ala Thr Asn Val 
            180                 185                 190         


Gln Gly His Asp Pro Asp Ile Asp Asn Leu Pro Leu Leu Ala Trp Ser 
        195                 200                 205             


Glu Asp Asp Val Thr Arg Ala Ser Pro Ile Ser Arg Lys Leu Ile Gln 
    210                 215                 220                 


Phe Gln Gln Tyr Tyr Phe Leu Val Ile Cys Ile Leu Leu Arg Phe Ile 
225                 230                 235                 240 


Trp Cys Phe Gln Ser Val Leu Thr Val Arg Ser Leu Lys Asp Arg Asp 
                245                 250                 255     


Asn Gln Phe Tyr Arg Ser Gln Tyr Lys Lys Glu Ala Ile Gly Leu Ala 
            260                 265                 270         


Leu His Trp Thr Leu Lys Thr Leu Phe His Leu Phe Phe Met Pro Ser 
        275                 280                 285             


Ile Leu Thr Ser Met Leu Val Phe Phe Val Ser Glu Leu Val Gly Gly 
    290                 295                 300                 


Phe Gly Ile Ala Ile Val Val Phe Met Asn His Tyr Pro Leu Glu Lys 
305                 310                 315                 320 


Ile Gly Asp Ser Val Trp Asp Gly His Gly Phe Ser Val Gly Gln Ile 
                325                 330                 335     


His Glu Thr Met Asn Ile Arg Arg Gly Ile Ile Thr Asp Trp Phe Phe 
            340                 345                 350         


Gly Gly Leu Asn Tyr Gln Ile Glu His His Leu Trp Pro Thr Leu Pro 
        355                 360                 365             


Arg His Asn Leu Thr Ala Val Ser Tyr Gln Val Glu Gln Leu Cys Gln 
    370                 375                 380                 


Lys His Asn Leu Pro Tyr Arg Asn Pro Leu Pro His Glu Gly Leu Val 
385                 390                 395                 400 


Ile Leu Leu Arg Tyr Leu Ser Gln Phe Ala Arg Met Ala Glu Lys Gln 
                405                 410                 415     


Pro Gly Ala Lys Ala Gln 
            420         


<210>  24
<211>  1260
<212>  DNA
<213>  Euglena anabaena UTEX 373


<220>
<221>  CDS
<222>  (1)..(1260)
<223>  delta-8 desaturase

<400>  24
atg gtg aaa agg cca gca ctt ccg ctg acc gtt gat ggt gtc acc tat       48
Met Val Lys Arg Pro Ala Leu Pro Leu Thr Val Asp Gly Val Thr Tyr         
1               5                   10                  15              

gat gtg tct gcc tgg ttg aac cat cat cca ggg ggt gct gac atc att       96
Asp Val Ser Ala Trp Leu Asn His His Pro Gly Gly Ala Asp Ile Ile         
            20                  25                  30                  

gag aac tac cgc ggt cgt gat gcc act gat gtc ttt atg gtt atg cac      144
Glu Asn Tyr Arg Gly Arg Asp Ala Thr Asp Val Phe Met Val Met His         
        35                  40                  45                      

tct gaa aat gct gtg agt aaa cta aga agg atg cct atc atg gaa cca      192
Ser Glu Asn Ala Val Ser Lys Leu Arg Arg Met Pro Ile Met Glu Pro         
    50                  55                  60                          

tca tct cca ctg acg cct acg cca ccg aaa ccc aac tca gac gaa ccg      240
Ser Ser Pro Leu Thr Pro Thr Pro Pro Lys Pro Asn Ser Asp Glu Pro         
65                  70                  75                  80          

cag gag gat ttc cgc aag ctc cga gat gag ctc atc gca gca gga atg      288
Gln Glu Asp Phe Arg Lys Leu Arg Asp Glu Leu Ile Ala Ala Gly Met         
                85                  90                  95              

ttc gac gca tca ccg atg tgg tac gca tat aag acg ctc agt acg ctg      336
Phe Asp Ala Ser Pro Met Trp Tyr Ala Tyr Lys Thr Leu Ser Thr Leu         
            100                 105                 110                 

ggc ctc ggg gtc ctc gcg gtg cta ttg atg acc cag tgg cac tgg tac      384
Gly Leu Gly Val Leu Ala Val Leu Leu Met Thr Gln Trp His Trp Tyr         
        115                 120                 125                     

ctc gtc ggg gca atc gtg ttg ggc att cac ttc caa caa atg ggt tgg      432
Leu Val Gly Ala Ile Val Leu Gly Ile His Phe Gln Gln Met Gly Trp         
    130                 135                 140                         

ttg tcg cac gat atc tgc cac cat cag ctg ttc aag gac cga tcg atc      480
Leu Ser His Asp Ile Cys His His Gln Leu Phe Lys Asp Arg Ser Ile         
145                 150                 155                 160         

aac aac gcc atc ggc ttg ctt ttc ggg aac gtc ttg caa ggg ttc tct      528
Asn Asn Ala Ile Gly Leu Leu Phe Gly Asn Val Leu Gln Gly Phe Ser         
                165                 170                 175             

gtg acc tgg tgg aag gac agg cac aat gca cac cac tcc gcc acc aac      576
Val Thr Trp Trp Lys Asp Arg His Asn Ala His His Ser Ala Thr Asn         
            180                 185                 190                 

gtg caa ggc cac gac ccc gac att gac aac ctg ccg ctg ctg gca tgg      624
Val Gln Gly His Asp Pro Asp Ile Asp Asn Leu Pro Leu Leu Ala Trp         
        195                 200                 205                     

tcc aag gag gac gtg gag agg gcc ggc ccg ttc tca cgg cgg atg atc      672
Ser Lys Glu Asp Val Glu Arg Ala Gly Pro Phe Ser Arg Arg Met Ile         
    210                 215                 220                         

aag tac cag caa tac tac ttc ttc ttc atc tgt gcc ctc ctg agg ttc      720
Lys Tyr Gln Gln Tyr Tyr Phe Phe Phe Ile Cys Ala Leu Leu Arg Phe         
225                 230                 235                 240         

atc tgg tgc ttc cag agc atc cac aca gcc acg ggc ctg aag gat cgc      768
Ile Trp Cys Phe Gln Ser Ile His Thr Ala Thr Gly Leu Lys Asp Arg         
                245                 250                 255             

agc aac cag tac tac cgc agg cag tac gag aaa gag agc gtg ggc ctg      816
Ser Asn Gln Tyr Tyr Arg Arg Gln Tyr Glu Lys Glu Ser Val Gly Leu         
            260                 265                 270                 

gcc ctc cac tgg ggc ctg aag gcg ttg ttc tac tac ttt tat atg cca      864
Ala Leu His Trp Gly Leu Lys Ala Leu Phe Tyr Tyr Phe Tyr Met Pro         
        275                 280                 285                     

agc ttc ttg acc gga ctc atg gtg ttt ttc gtg tcc gag ttg ctt ggg      912
Ser Phe Leu Thr Gly Leu Met Val Phe Phe Val Ser Glu Leu Leu Gly         
    290                 295                 300                         

ggc ttc ggc atc gcc atc gtg gtg ttc atg aac cac tac ccc ctg gag      960
Gly Phe Gly Ile Ala Ile Val Val Phe Met Asn His Tyr Pro Leu Glu         
305                 310                 315                 320         

aag atc cag gac tcg gtg tgg gac ggc cac ggc ttt tgc gcc ggc cag     1008
Lys Ile Gln Asp Ser Val Trp Asp Gly His Gly Phe Cys Ala Gly Gln         
                325                 330                 335             

att cac gaa acg atg aac gtc cag cgg gga ctc gtc acg gac tgg ttc     1056
Ile His Glu Thr Met Asn Val Gln Arg Gly Leu Val Thr Asp Trp Phe         
            340                 345                 350                 

ttc ggt ggg ctg aat tac caa atc gag cac cac ctg tgg ccg acg ctg     1104
Phe Gly Gly Leu Asn Tyr Gln Ile Glu His His Leu Trp Pro Thr Leu         
        355                 360                 365                     

ccc cgg cac aac ctg acg gcg gcc agc atc aaa gtg gag cag ttg tgc     1152
Pro Arg His Asn Leu Thr Ala Ala Ser Ile Lys Val Glu Gln Leu Cys         
    370                 375                 380                         

aag aag cac aac ttg ccg tat cgc agc ccc cca atg ctg gag ggg gtg     1200
Lys Lys His Asn Leu Pro Tyr Arg Ser Pro Pro Met Leu Glu Gly Val         
385                 390                 395                 400         

ggc atc ctg atc agc tac ctg ggc acc ttt gcc cgc atg gtg gca aag     1248
Gly Ile Leu Ile Ser Tyr Leu Gly Thr Phe Ala Arg Met Val Ala Lys         
                405                 410                 415             

gcc gac aag gcg                                                     1260
Ala Asp Lys Ala                                                         
            420                                                         


<210>  25
<211>  420
<212>  PRT
<213>  Euglena anabaena UTEX 373

<400>  25

Met Val Lys Arg Pro Ala Leu Pro Leu Thr Val Asp Gly Val Thr Tyr 
1               5                   10                  15      


Asp Val Ser Ala Trp Leu Asn His His Pro Gly Gly Ala Asp Ile Ile 
            20                  25                  30          


Glu Asn Tyr Arg Gly Arg Asp Ala Thr Asp Val Phe Met Val Met His 
        35                  40                  45              


Ser Glu Asn Ala Val Ser Lys Leu Arg Arg Met Pro Ile Met Glu Pro 
    50                  55                  60                  


Ser Ser Pro Leu Thr Pro Thr Pro Pro Lys Pro Asn Ser Asp Glu Pro 
65                  70                  75                  80  


Gln Glu Asp Phe Arg Lys Leu Arg Asp Glu Leu Ile Ala Ala Gly Met 
                85                  90                  95      


Phe Asp Ala Ser Pro Met Trp Tyr Ala Tyr Lys Thr Leu Ser Thr Leu 
            100                 105                 110         


Gly Leu Gly Val Leu Ala Val Leu Leu Met Thr Gln Trp His Trp Tyr 
        115                 120                 125             


Leu Val Gly Ala Ile Val Leu Gly Ile His Phe Gln Gln Met Gly Trp 
    130                 135                 140                 


Leu Ser His Asp Ile Cys His His Gln Leu Phe Lys Asp Arg Ser Ile 
145                 150                 155                 160 


Asn Asn Ala Ile Gly Leu Leu Phe Gly Asn Val Leu Gln Gly Phe Ser 
                165                 170                 175     


Val Thr Trp Trp Lys Asp Arg His Asn Ala His His Ser Ala Thr Asn 
            180                 185                 190         


Val Gln Gly His Asp Pro Asp Ile Asp Asn Leu Pro Leu Leu Ala Trp 
        195                 200                 205             


Ser Lys Glu Asp Val Glu Arg Ala Gly Pro Phe Ser Arg Arg Met Ile 
    210                 215                 220                 


Lys Tyr Gln Gln Tyr Tyr Phe Phe Phe Ile Cys Ala Leu Leu Arg Phe 
225                 230                 235                 240 


Ile Trp Cys Phe Gln Ser Ile His Thr Ala Thr Gly Leu Lys Asp Arg 
                245                 250                 255     


Ser Asn Gln Tyr Tyr Arg Arg Gln Tyr Glu Lys Glu Ser Val Gly Leu 
            260                 265                 270         


Ala Leu His Trp Gly Leu Lys Ala Leu Phe Tyr Tyr Phe Tyr Met Pro 
        275                 280                 285             


Ser Phe Leu Thr Gly Leu Met Val Phe Phe Val Ser Glu Leu Leu Gly 
    290                 295                 300                 


Gly Phe Gly Ile Ala Ile Val Val Phe Met Asn His Tyr Pro Leu Glu 
305                 310                 315                 320 


Lys Ile Gln Asp Ser Val Trp Asp Gly His Gly Phe Cys Ala Gly Gln 
                325                 330                 335     


Ile His Glu Thr Met Asn Val Gln Arg Gly Leu Val Thr Asp Trp Phe 
            340                 345                 350         


Phe Gly Gly Leu Asn Tyr Gln Ile Glu His His Leu Trp Pro Thr Leu 
        355                 360                 365             


Pro Arg His Asn Leu Thr Ala Ala Ser Ile Lys Val Glu Gln Leu Cys 
    370                 375                 380                 


Lys Lys His Asn Leu Pro Tyr Arg Ser Pro Pro Met Leu Glu Gly Val 
385                 390                 395                 400 


Gly Ile Leu Ile Ser Tyr Leu Gly Thr Phe Ala Arg Met Val Ala Lys 
                405                 410                 415     


Ala Asp Lys Ala 
            420 


<210>  26
<211>  1260
<212>  DNA
<213>  Euglena anabaena UTEX 373


<220>
<221>  CDS
<222>  (1)..(1260)
<223>  synthetic delta-8 desaturase (codon-optimized for Yarrowia 
       lipolytica)

<400>  26
atg gtc aag cga ccc gct ctg cct ctc acc gtg gac ggt gtc acc tac       48
Met Val Lys Arg Pro Ala Leu Pro Leu Thr Val Asp Gly Val Thr Tyr         
1               5                   10                  15              

gac gtt tct gcc tgg ctc aac cac cat ccc gga ggt gcc gac att atc       96
Asp Val Ser Ala Trp Leu Asn His His Pro Gly Gly Ala Asp Ile Ile         
            20                  25                  30                  

gag aac tac cga ggt cgg gat gct acc gac gtc ttc atg gtt atg cac      144
Glu Asn Tyr Arg Gly Arg Asp Ala Thr Asp Val Phe Met Val Met His         
        35                  40                  45                      

tcc gag aac gcc gtg tcc aaa ctc aga cga atg ccc atc atg gaa cct      192
Ser Glu Asn Ala Val Ser Lys Leu Arg Arg Met Pro Ile Met Glu Pro         
    50                  55                  60                          

tcc tct ccc ctg act cca aca cct ccc aag cca aac tcc gac gaa cct      240
Ser Ser Pro Leu Thr Pro Thr Pro Pro Lys Pro Asn Ser Asp Glu Pro         
65                  70                  75                  80          

cag gag gat ttc cga aag ctg cga gac gag ctc att gct gca ggc atg      288
Gln Glu Asp Phe Arg Lys Leu Arg Asp Glu Leu Ile Ala Ala Gly Met         
                85                  90                  95              

ttc gat gcc tct ccc atg tgg tac gct tac aag acc ctg tcg act ctc      336
Phe Asp Ala Ser Pro Met Trp Tyr Ala Tyr Lys Thr Leu Ser Thr Leu         
            100                 105                 110                 

gga ctg ggt gtc ctt gcc gtg ctg ttg atg acc cag tgg cac tgg tac      384
Gly Leu Gly Val Leu Ala Val Leu Leu Met Thr Gln Trp His Trp Tyr         
        115                 120                 125                     

ctg gtt ggt gct atc gtc ctc ggc att cac ttt caa cag atg gga tgg      432
Leu Val Gly Ala Ile Val Leu Gly Ile His Phe Gln Gln Met Gly Trp         
    130                 135                 140                         

ctc tcg cac gac att tgc cat cac cag ctg ttc aag gac cga tcc atc      480
Leu Ser His Asp Ile Cys His His Gln Leu Phe Lys Asp Arg Ser Ile         
145                 150                 155                 160         

aac aat gcc att ggc ctg ctc ttc gga aac gtg ctt cag ggc ttt tct      528
Asn Asn Ala Ile Gly Leu Leu Phe Gly Asn Val Leu Gln Gly Phe Ser         
                165                 170                 175             

gtc act tgg tgg aag gac cga cac aac gct cat cac tcc gcc acc aac      576
Val Thr Trp Trp Lys Asp Arg His Asn Ala His His Ser Ala Thr Asn         
            180                 185                 190                 

gtg cag ggt cac gat ccc gac atc gac aac ctg cct ctc ctg gcg tgg      624
Val Gln Gly His Asp Pro Asp Ile Asp Asn Leu Pro Leu Leu Ala Trp         
        195                 200                 205                     

tcc aag gag gac gtc gag cga gct ggc ccg ttt tct cga cgg atg atc      672
Ser Lys Glu Asp Val Glu Arg Ala Gly Pro Phe Ser Arg Arg Met Ile         
    210                 215                 220                         

aag tac caa cag tat tac ttc ttt ttc atc tgt gcc ctt ctg cga ttc      720
Lys Tyr Gln Gln Tyr Tyr Phe Phe Phe Ile Cys Ala Leu Leu Arg Phe         
225                 230                 235                 240         

atc tgg tgc ttt cag tcc att cat act gcc acg ggt ctc aag gat cga      768
Ile Trp Cys Phe Gln Ser Ile His Thr Ala Thr Gly Leu Lys Asp Arg         
                245                 250                 255             

agc aat cag tac tat cga aga cag tac gag aag gag tcc gtc ggt ctg      816
Ser Asn Gln Tyr Tyr Arg Arg Gln Tyr Glu Lys Glu Ser Val Gly Leu         
            260                 265                 270                 

gca ctc cac tgg ggt ctc aag gcc ttg ttc tac tat ttc tac atg ccc      864
Ala Leu His Trp Gly Leu Lys Ala Leu Phe Tyr Tyr Phe Tyr Met Pro         
        275                 280                 285                     

tcg ttt ctc acc gga ctc atg gtg ttc ttt gtc tcc gag ctg ctt ggt      912
Ser Phe Leu Thr Gly Leu Met Val Phe Phe Val Ser Glu Leu Leu Gly         
    290                 295                 300                         

ggc ttc gga att gcc atc gtt gtc ttc atg aac cac tac cct ctg gag      960
Gly Phe Gly Ile Ala Ile Val Val Phe Met Asn His Tyr Pro Leu Glu         
305                 310                 315                 320         

aag att cag gac tcc gtg tgg gat ggt cat ggc ttc tgt gct gga cag     1008
Lys Ile Gln Asp Ser Val Trp Asp Gly His Gly Phe Cys Ala Gly Gln         
                325                 330                 335             

att cac gag acc atg aac gtt cag cga ggc ctc gtc aca gac tgg ttt     1056
Ile His Glu Thr Met Asn Val Gln Arg Gly Leu Val Thr Asp Trp Phe         
            340                 345                 350                 

ttc ggt ggc ctc aac tac cag atc gaa cat cac ctg tgg cct act ctt     1104
Phe Gly Gly Leu Asn Tyr Gln Ile Glu His His Leu Trp Pro Thr Leu         
        355                 360                 365                     

ccc aga cac aac ctc acc gct gcc tcc atc aaa gtg gag cag ctg tgc     1152
Pro Arg His Asn Leu Thr Ala Ala Ser Ile Lys Val Glu Gln Leu Cys         
    370                 375                 380                         

aag aag cac aac ctg ccc tac cga tct cct ccc atg ctc gaa ggt gtc     1200
Lys Lys His Asn Leu Pro Tyr Arg Ser Pro Pro Met Leu Glu Gly Val         
385                 390                 395                 400         

ggc att ctt atc tcc tac ctg ggc acc ttc gct cga atg gtt gcc aag     1248
Gly Ile Leu Ile Ser Tyr Leu Gly Thr Phe Ala Arg Met Val Ala Lys         
                405                 410                 415             

gca gac aag gcc                                                     1260
Ala Asp Lys Ala                                                         
            420                                                         


<210>  27
<211>  420
<212>  PRT
<213>  Euglena anabaena UTEX 373

<400>  27

Met Val Lys Arg Pro Ala Leu Pro Leu Thr Val Asp Gly Val Thr Tyr 
1               5                   10                  15      


Asp Val Ser Ala Trp Leu Asn His His Pro Gly Gly Ala Asp Ile Ile 
            20                  25                  30          


Glu Asn Tyr Arg Gly Arg Asp Ala Thr Asp Val Phe Met Val Met His 
        35                  40                  45              


Ser Glu Asn Ala Val Ser Lys Leu Arg Arg Met Pro Ile Met Glu Pro 
    50                  55                  60                  


Ser Ser Pro Leu Thr Pro Thr Pro Pro Lys Pro Asn Ser Asp Glu Pro 
65                  70                  75                  80  


Gln Glu Asp Phe Arg Lys Leu Arg Asp Glu Leu Ile Ala Ala Gly Met 
                85                  90                  95      


Phe Asp Ala Ser Pro Met Trp Tyr Ala Tyr Lys Thr Leu Ser Thr Leu 
            100                 105                 110         


Gly Leu Gly Val Leu Ala Val Leu Leu Met Thr Gln Trp His Trp Tyr 
        115                 120                 125             


Leu Val Gly Ala Ile Val Leu Gly Ile His Phe Gln Gln Met Gly Trp 
    130                 135                 140                 


Leu Ser His Asp Ile Cys His His Gln Leu Phe Lys Asp Arg Ser Ile 
145                 150                 155                 160 


Asn Asn Ala Ile Gly Leu Leu Phe Gly Asn Val Leu Gln Gly Phe Ser 
                165                 170                 175     


Val Thr Trp Trp Lys Asp Arg His Asn Ala His His Ser Ala Thr Asn 
            180                 185                 190         


Val Gln Gly His Asp Pro Asp Ile Asp Asn Leu Pro Leu Leu Ala Trp 
        195                 200                 205             


Ser Lys Glu Asp Val Glu Arg Ala Gly Pro Phe Ser Arg Arg Met Ile 
    210                 215                 220                 


Lys Tyr Gln Gln Tyr Tyr Phe Phe Phe Ile Cys Ala Leu Leu Arg Phe 
225                 230                 235                 240 


Ile Trp Cys Phe Gln Ser Ile His Thr Ala Thr Gly Leu Lys Asp Arg 
                245                 250                 255     


Ser Asn Gln Tyr Tyr Arg Arg Gln Tyr Glu Lys Glu Ser Val Gly Leu 
            260                 265                 270         


Ala Leu His Trp Gly Leu Lys Ala Leu Phe Tyr Tyr Phe Tyr Met Pro 
        275                 280                 285             


Ser Phe Leu Thr Gly Leu Met Val Phe Phe Val Ser Glu Leu Leu Gly 
    290                 295                 300                 


Gly Phe Gly Ile Ala Ile Val Val Phe Met Asn His Tyr Pro Leu Glu 
305                 310                 315                 320 


Lys Ile Gln Asp Ser Val Trp Asp Gly His Gly Phe Cys Ala Gly Gln 
                325                 330                 335     


Ile His Glu Thr Met Asn Val Gln Arg Gly Leu Val Thr Asp Trp Phe 
            340                 345                 350         


Phe Gly Gly Leu Asn Tyr Gln Ile Glu His His Leu Trp Pro Thr Leu 
        355                 360                 365             


Pro Arg His Asn Leu Thr Ala Ala Ser Ile Lys Val Glu Gln Leu Cys 
    370                 375                 380                 


Lys Lys His Asn Leu Pro Tyr Arg Ser Pro Pro Met Leu Glu Gly Val 
385                 390                 395                 400 


Gly Ile Leu Ile Ser Tyr Leu Gly Thr Phe Ala Arg Met Val Ala Lys 
                405                 410                 415     


Ala Asp Lys Ala 
            420 


<210>  28
<211>  1260
<212>  DNA
<213>  Euglena anabaena UTEX 373


<220>
<221>  CDS
<222>  (1)..(1260)
<223>  delta-8 desaturase

<400>  28
atg gtg aaa agg cca gca ctt ccg ctg acc gtt gat ggt gtc acc tat       48
Met Val Lys Arg Pro Ala Leu Pro Leu Thr Val Asp Gly Val Thr Tyr         
1               5                   10                  15              

gat gtg tct gcc tgg ttg aac cat cat cca ggg ggt gct gac atc att       96
Asp Val Ser Ala Trp Leu Asn His His Pro Gly Gly Ala Asp Ile Ile         
            20                  25                  30                  

gag aac tac cgc ggt cgt gat gcc act gat gtc ttt atg gtt atg cac      144
Glu Asn Tyr Arg Gly Arg Asp Ala Thr Asp Val Phe Met Val Met His         
        35                  40                  45                      

tct gaa aat gct gtg agt aaa cta aga agg atg cct atc atg gaa cca      192
Ser Glu Asn Ala Val Ser Lys Leu Arg Arg Met Pro Ile Met Glu Pro         
    50                  55                  60                          

tca tct cca ctg acg cct acg cca ccg aaa ccc aac tca gac gaa ccg      240
Ser Ser Pro Leu Thr Pro Thr Pro Pro Lys Pro Asn Ser Asp Glu Pro         
65                  70                  75                  80          

cag gag gat ttc cgc aag ctc cga gat gag ctc atc gca gca gga atg      288
Gln Glu Asp Phe Arg Lys Leu Arg Asp Glu Leu Ile Ala Ala Gly Met         
                85                  90                  95              

ttc gac gca tca ccg atg tgg tac gca tat aag acg ctc act acg ctg      336
Phe Asp Ala Ser Pro Met Trp Tyr Ala Tyr Lys Thr Leu Thr Thr Leu         
            100                 105                 110                 

ggc ctc ggg gtc ctc gcg gtg cta ttg atg acc cag tgg cac tgg tac      384
Gly Leu Gly Val Leu Ala Val Leu Leu Met Thr Gln Trp His Trp Tyr         
        115                 120                 125                     

ctc gtc ggg gca atc gtg ttg ggc att cac ttc caa caa atg ggt tgg      432
Leu Val Gly Ala Ile Val Leu Gly Ile His Phe Gln Gln Met Gly Trp         
    130                 135                 140                         

ttg tcg cac gat atc tgc cac cat cag ctg ttc aag gac cga tcg atc      480
Leu Ser His Asp Ile Cys His His Gln Leu Phe Lys Asp Arg Ser Ile         
145                 150                 155                 160         

aac aac gcc atc ggc ttg ctt ttc ggg aac gtc ttg caa ggg ttc tct      528
Asn Asn Ala Ile Gly Leu Leu Phe Gly Asn Val Leu Gln Gly Phe Ser         
                165                 170                 175             

gtg acc tgg tgg aag gac agg cac aat gca cac cac tcc gcc acc aac      576
Val Thr Trp Trp Lys Asp Arg His Asn Ala His His Ser Ala Thr Asn         
            180                 185                 190                 

gtg caa ggc cac gac ccc gac att gac aac ctg ccg ctg ctg gca tgg      624
Val Gln Gly His Asp Pro Asp Ile Asp Asn Leu Pro Leu Leu Ala Trp         
        195                 200                 205                     

tcc aag gag gac gtg gag agg gcc ggc ccg ttc tca cgg cgg atg atc      672
Ser Lys Glu Asp Val Glu Arg Ala Gly Pro Phe Ser Arg Arg Met Ile         
    210                 215                 220                         

aag tac cag caa tac tac ttc ttc ttc atc tgt gcc ctc ctg agg ttc      720
Lys Tyr Gln Gln Tyr Tyr Phe Phe Phe Ile Cys Ala Leu Leu Arg Phe         
225                 230                 235                 240         

atc tgg tgc ttc cag agc atc cac aca gcc aag ggc ctg aag gat cgc      768
Ile Trp Cys Phe Gln Ser Ile His Thr Ala Lys Gly Leu Lys Asp Arg         
                245                 250                 255             

agc aac cag tac tac cgc agg cag tac gag aaa gag agc gtg ggc ctg      816
Ser Asn Gln Tyr Tyr Arg Arg Gln Tyr Glu Lys Glu Ser Val Gly Leu         
            260                 265                 270                 

gcc ctc cac tgg ggc ctg aag gcg ttg ttc tac tac ttt tat atg cca      864
Ala Leu His Trp Gly Leu Lys Ala Leu Phe Tyr Tyr Phe Tyr Met Pro         
        275                 280                 285                     

agc ttc ttg acc gga ctc atg gtg ttt ttc gtg tcc gag ttg ctt ggg      912
Ser Phe Leu Thr Gly Leu Met Val Phe Phe Val Ser Glu Leu Leu Gly         
    290                 295                 300                         

ggc ttc ggc atc gcc atc gtg gtg ttc atg aac cac tac ccc ctg gag      960
Gly Phe Gly Ile Ala Ile Val Val Phe Met Asn His Tyr Pro Leu Glu         
305                 310                 315                 320         

aag atc cag gac tcg gtg tgg gac ggc cac ggc ttt tgc gcc ggc cag     1008
Lys Ile Gln Asp Ser Val Trp Asp Gly His Gly Phe Cys Ala Gly Gln         
                325                 330                 335             

att cac gaa acg atg aac gtc cag cgg gga ctc gtc acg gac tgg ttc     1056
Ile His Glu Thr Met Asn Val Gln Arg Gly Leu Val Thr Asp Trp Phe         
            340                 345                 350                 

ttc ggt ggg ctg aat tac caa atc gag cac cac ctg tgg ccg acg ctg     1104
Phe Gly Gly Leu Asn Tyr Gln Ile Glu His His Leu Trp Pro Thr Leu         
        355                 360                 365                     

ccc cgg cac aac ctg acg gcg gcc agc atc aaa gtg gag cag ttg tgc     1152
Pro Arg His Asn Leu Thr Ala Ala Ser Ile Lys Val Glu Gln Leu Cys         
    370                 375                 380                         

aag aag cac aac ttg ccg tat cgc agc ccc cca atg ctg gag ggg gtg     1200
Lys Lys His Asn Leu Pro Tyr Arg Ser Pro Pro Met Leu Glu Gly Val         
385                 390                 395                 400         

ggc atc ctg atc agc tac ctg ggc acc ttt gcc cgc atg gtg gca aag     1248
Gly Ile Leu Ile Ser Tyr Leu Gly Thr Phe Ala Arg Met Val Ala Lys         
                405                 410                 415             

gcc gac aag gcg                                                     1260
Ala Asp Lys Ala                                                         
            420                                                         


<210>  29
<211>  420
<212>  PRT
<213>  Euglena anabaena UTEX 373

<400>  29

Met Val Lys Arg Pro Ala Leu Pro Leu Thr Val Asp Gly Val Thr Tyr 
1               5                   10                  15      


Asp Val Ser Ala Trp Leu Asn His His Pro Gly Gly Ala Asp Ile Ile 
            20                  25                  30          


Glu Asn Tyr Arg Gly Arg Asp Ala Thr Asp Val Phe Met Val Met His 
        35                  40                  45              


Ser Glu Asn Ala Val Ser Lys Leu Arg Arg Met Pro Ile Met Glu Pro 
    50                  55                  60                  


Ser Ser Pro Leu Thr Pro Thr Pro Pro Lys Pro Asn Ser Asp Glu Pro 
65                  70                  75                  80  


Gln Glu Asp Phe Arg Lys Leu Arg Asp Glu Leu Ile Ala Ala Gly Met 
                85                  90                  95      


Phe Asp Ala Ser Pro Met Trp Tyr Ala Tyr Lys Thr Leu Thr Thr Leu 
            100                 105                 110         


Gly Leu Gly Val Leu Ala Val Leu Leu Met Thr Gln Trp His Trp Tyr 
        115                 120                 125             


Leu Val Gly Ala Ile Val Leu Gly Ile His Phe Gln Gln Met Gly Trp 
    130                 135                 140                 


Leu Ser His Asp Ile Cys His His Gln Leu Phe Lys Asp Arg Ser Ile 
145                 150                 155                 160 


Asn Asn Ala Ile Gly Leu Leu Phe Gly Asn Val Leu Gln Gly Phe Ser 
                165                 170                 175     


Val Thr Trp Trp Lys Asp Arg His Asn Ala His His Ser Ala Thr Asn 
            180                 185                 190         


Val Gln Gly His Asp Pro Asp Ile Asp Asn Leu Pro Leu Leu Ala Trp 
        195                 200                 205             


Ser Lys Glu Asp Val Glu Arg Ala Gly Pro Phe Ser Arg Arg Met Ile 
    210                 215                 220                 


Lys Tyr Gln Gln Tyr Tyr Phe Phe Phe Ile Cys Ala Leu Leu Arg Phe 
225                 230                 235                 240 


Ile Trp Cys Phe Gln Ser Ile His Thr Ala Lys Gly Leu Lys Asp Arg 
                245                 250                 255     


Ser Asn Gln Tyr Tyr Arg Arg Gln Tyr Glu Lys Glu Ser Val Gly Leu 
            260                 265                 270         


Ala Leu His Trp Gly Leu Lys Ala Leu Phe Tyr Tyr Phe Tyr Met Pro 
        275                 280                 285             


Ser Phe Leu Thr Gly Leu Met Val Phe Phe Val Ser Glu Leu Leu Gly 
    290                 295                 300                 


Gly Phe Gly Ile Ala Ile Val Val Phe Met Asn His Tyr Pro Leu Glu 
305                 310                 315                 320 


Lys Ile Gln Asp Ser Val Trp Asp Gly His Gly Phe Cys Ala Gly Gln 
                325                 330                 335     


Ile His Glu Thr Met Asn Val Gln Arg Gly Leu Val Thr Asp Trp Phe 
            340                 345                 350         


Phe Gly Gly Leu Asn Tyr Gln Ile Glu His His Leu Trp Pro Thr Leu 
        355                 360                 365             


Pro Arg His Asn Leu Thr Ala Ala Ser Ile Lys Val Glu Gln Leu Cys 
    370                 375                 380                 


Lys Lys His Asn Leu Pro Tyr Arg Ser Pro Pro Met Leu Glu Gly Val 
385                 390                 395                 400 


Gly Ile Leu Ile Ser Tyr Leu Gly Thr Phe Ala Arg Met Val Ala Lys 
                405                 410                 415     


Ala Asp Lys Ala 
            420 


<210>  30
<211>  1260
<212>  DNA
<213>  Euglena anabaena UTEX 373


<220>
<221>  CDS
<222>  (1)..(1260)
<223>  delta-8 desaturase

<400>  30
atg gtg aaa agg cca gca ctt ccg ctg acc gtt gat ggt gtc acc tat       48
Met Val Lys Arg Pro Ala Leu Pro Leu Thr Val Asp Gly Val Thr Tyr         
1               5                   10                  15              

gat gtg tct gcc tgg ttg aac cat cat cca ggg ggt gct gac atc att       96
Asp Val Ser Ala Trp Leu Asn His His Pro Gly Gly Ala Asp Ile Ile         
            20                  25                  30                  

gag aac tac cgc ggt cgt gat gcc act gat gtc ttt atg gtt atg cac      144
Glu Asn Tyr Arg Gly Arg Asp Ala Thr Asp Val Phe Met Val Met His         
        35                  40                  45                      

tct gaa aat gct gtg agt aaa cta aga agg atg cct atc atg gaa cca      192
Ser Glu Asn Ala Val Ser Lys Leu Arg Arg Met Pro Ile Met Glu Pro         
    50                  55                  60                          

tca tct cca ctg acg cct acg cca ccg aaa ccc aac tca gac gaa ccg      240
Ser Ser Pro Leu Thr Pro Thr Pro Pro Lys Pro Asn Ser Asp Glu Pro         
65                  70                  75                  80          

cag gag gat ttc cgc aag ctc cga gat gag ctc atc gca gca gga atg      288
Gln Glu Asp Phe Arg Lys Leu Arg Asp Glu Leu Ile Ala Ala Gly Met         
                85                  90                  95              

ttc gac gca tca ccg atg tgg tac gca tat aag acg ctc agt acg ctg      336
Phe Asp Ala Ser Pro Met Trp Tyr Ala Tyr Lys Thr Leu Ser Thr Leu         
            100                 105                 110                 

ggc ctc ggg gtc ctc gcg gtg cta ttg atg acc cag tgg cac tgg tac      384
Gly Leu Gly Val Leu Ala Val Leu Leu Met Thr Gln Trp His Trp Tyr         
        115                 120                 125                     

ctc gtc ggg gca atc gtg ttg ggc att cac ttc caa caa atg ggt tgg      432
Leu Val Gly Ala Ile Val Leu Gly Ile His Phe Gln Gln Met Gly Trp         
    130                 135                 140                         

ttg tcg cac gat atc tgc cac cat cag ctg ttc aag gac cga tcg atc      480
Leu Ser His Asp Ile Cys His His Gln Leu Phe Lys Asp Arg Ser Ile         
145                 150                 155                 160         

aac aac gcc atc ggc ttg ctt ttc ggg aac gtc ttg caa ggg ttc tct      528
Asn Asn Ala Ile Gly Leu Leu Phe Gly Asn Val Leu Gln Gly Phe Ser         
                165                 170                 175             

gtg acc tgg tgg aag gac agg cac aat gca cac cac tcc gcc acc aac      576
Val Thr Trp Trp Lys Asp Arg His Asn Ala His His Ser Ala Thr Asn         
            180                 185                 190                 

gtg caa ggc cac gac ccc gac att gac aac ctg ccg ctg ctg gca tgg      624
Val Gln Gly His Asp Pro Asp Ile Asp Asn Leu Pro Leu Leu Ala Trp         
        195                 200                 205                     

tcc aag gag gac gtg gag agg gcc ggc ccg ttc tca cgg cgg att atc      672
Ser Lys Glu Asp Val Glu Arg Ala Gly Pro Phe Ser Arg Arg Ile Ile         
    210                 215                 220                         

aag tac cag caa tac tac ttc ttc ttc atc tgt gcc ctc ctg agg ttc      720
Lys Tyr Gln Gln Tyr Tyr Phe Phe Phe Ile Cys Ala Leu Leu Arg Phe         
225                 230                 235                 240         

atc tgg tgc ttc cag agc atc cac aca gcc acg ggc ctg aag gat cgc      768
Ile Trp Cys Phe Gln Ser Ile His Thr Ala Thr Gly Leu Lys Asp Arg         
                245                 250                 255             

agc aac cag tac tac cgc agg cag tac gag aaa gag agc gtg ggc ctg      816
Ser Asn Gln Tyr Tyr Arg Arg Gln Tyr Glu Lys Glu Ser Val Gly Leu         
            260                 265                 270                 

gcc ctc cac tgg ggc ctg aag gcg ttg ttc tac tac ttt tat atg cca      864
Ala Leu His Trp Gly Leu Lys Ala Leu Phe Tyr Tyr Phe Tyr Met Pro         
        275                 280                 285                     

agc ttc ttg acc gga ctc atg gtg ttt ttc gtg tcc gag ttg ctt ggg      912
Ser Phe Leu Thr Gly Leu Met Val Phe Phe Val Ser Glu Leu Leu Gly         
    290                 295                 300                         

ggc ttc ggc atc gcc atc gtg gtg ttc atg aac cac tac ccc ctg gag      960
Gly Phe Gly Ile Ala Ile Val Val Phe Met Asn His Tyr Pro Leu Glu         
305                 310                 315                 320         

aag atc cag gac tcg gtg tgg gac ggc cac ggc ttt tgc gcc ggc cag     1008
Lys Ile Gln Asp Ser Val Trp Asp Gly His Gly Phe Cys Ala Gly Gln         
                325                 330                 335             

att cac gaa acg atg aac gtc cag cgg gga ctc gtc acg gac tgg ttc     1056
Ile His Glu Thr Met Asn Val Gln Arg Gly Leu Val Thr Asp Trp Phe         
            340                 345                 350                 

ttc ggt ggg ctg aat tac caa atc gag cac cac ctg tgg ccg acg ctg     1104
Phe Gly Gly Leu Asn Tyr Gln Ile Glu His His Leu Trp Pro Thr Leu         
        355                 360                 365                     

ccc cgg cac aac ctg acg gcg gcc agc atc aaa gtg gag cag ttg tgc     1152
Pro Arg His Asn Leu Thr Ala Ala Ser Ile Lys Val Glu Gln Leu Cys         
    370                 375                 380                         

aag aag cac aac ttg ccg tat cgc agc ccc cca atg ctg gag ggg gtg     1200
Lys Lys His Asn Leu Pro Tyr Arg Ser Pro Pro Met Leu Glu Gly Val         
385                 390                 395                 400         

ggc atc ctg atc agc tac ctg ggc acc ttt gcc cgc atg gtg gca aag     1248
Gly Ile Leu Ile Ser Tyr Leu Gly Thr Phe Ala Arg Met Val Ala Lys         
                405                 410                 415             

gcc gac aag gcg                                                     1260
Ala Asp Lys Ala                                                         
            420                                                         


<210>  31
<211>  420
<212>  PRT
<213>  Euglena anabaena UTEX 373

<400>  31

Met Val Lys Arg Pro Ala Leu Pro Leu Thr Val Asp Gly Val Thr Tyr 
1               5                   10                  15      


Asp Val Ser Ala Trp Leu Asn His His Pro Gly Gly Ala Asp Ile Ile 
            20                  25                  30          


Glu Asn Tyr Arg Gly Arg Asp Ala Thr Asp Val Phe Met Val Met His 
        35                  40                  45              


Ser Glu Asn Ala Val Ser Lys Leu Arg Arg Met Pro Ile Met Glu Pro 
    50                  55                  60                  


Ser Ser Pro Leu Thr Pro Thr Pro Pro Lys Pro Asn Ser Asp Glu Pro 
65                  70                  75                  80  


Gln Glu Asp Phe Arg Lys Leu Arg Asp Glu Leu Ile Ala Ala Gly Met 
                85                  90                  95      


Phe Asp Ala Ser Pro Met Trp Tyr Ala Tyr Lys Thr Leu Ser Thr Leu 
            100                 105                 110         


Gly Leu Gly Val Leu Ala Val Leu Leu Met Thr Gln Trp His Trp Tyr 
        115                 120                 125             


Leu Val Gly Ala Ile Val Leu Gly Ile His Phe Gln Gln Met Gly Trp 
    130                 135                 140                 


Leu Ser His Asp Ile Cys His His Gln Leu Phe Lys Asp Arg Ser Ile 
145                 150                 155                 160 


Asn Asn Ala Ile Gly Leu Leu Phe Gly Asn Val Leu Gln Gly Phe Ser 
                165                 170                 175     


Val Thr Trp Trp Lys Asp Arg His Asn Ala His His Ser Ala Thr Asn 
            180                 185                 190         


Val Gln Gly His Asp Pro Asp Ile Asp Asn Leu Pro Leu Leu Ala Trp 
        195                 200                 205             


Ser Lys Glu Asp Val Glu Arg Ala Gly Pro Phe Ser Arg Arg Ile Ile 
    210                 215                 220                 


Lys Tyr Gln Gln Tyr Tyr Phe Phe Phe Ile Cys Ala Leu Leu Arg Phe 
225                 230                 235                 240 


Ile Trp Cys Phe Gln Ser Ile His Thr Ala Thr Gly Leu Lys Asp Arg 
                245                 250                 255     


Ser Asn Gln Tyr Tyr Arg Arg Gln Tyr Glu Lys Glu Ser Val Gly Leu 
            260                 265                 270         


Ala Leu His Trp Gly Leu Lys Ala Leu Phe Tyr Tyr Phe Tyr Met Pro 
        275                 280                 285             


Ser Phe Leu Thr Gly Leu Met Val Phe Phe Val Ser Glu Leu Leu Gly 
    290                 295                 300                 


Gly Phe Gly Ile Ala Ile Val Val Phe Met Asn His Tyr Pro Leu Glu 
305                 310                 315                 320 


Lys Ile Gln Asp Ser Val Trp Asp Gly His Gly Phe Cys Ala Gly Gln 
                325                 330                 335     


Ile His Glu Thr Met Asn Val Gln Arg Gly Leu Val Thr Asp Trp Phe 
            340                 345                 350         


Phe Gly Gly Leu Asn Tyr Gln Ile Glu His His Leu Trp Pro Thr Leu 
        355                 360                 365             


Pro Arg His Asn Leu Thr Ala Ala Ser Ile Lys Val Glu Gln Leu Cys 
    370                 375                 380                 


Lys Lys His Asn Leu Pro Tyr Arg Ser Pro Pro Met Leu Glu Gly Val 
385                 390                 395                 400 


Gly Ile Leu Ile Ser Tyr Leu Gly Thr Phe Ala Arg Met Val Ala Lys 
                405                 410                 415     


Ala Asp Lys Ala 
            420 


<210>  32
<211>  1260
<212>  DNA
<213>  Euglena anabaena UTEX 373


<220>
<221>  CDS
<222>  (1)..(1260)
<223>  delta-8 desaturase

<400>  32
atg gtg aaa agg cca gca ctt ccg ctg acc gtt gat ggt gtc acc tat       48
Met Val Lys Arg Pro Ala Leu Pro Leu Thr Val Asp Gly Val Thr Tyr         
1               5                   10                  15              

gat gtg tct gcc tgg ttg aac cat cat cca ggg ggt gct gac atc att       96
Asp Val Ser Ala Trp Leu Asn His His Pro Gly Gly Ala Asp Ile Ile         
            20                  25                  30                  

gag aac tac cgc ggt cgt gat gcc act gat gtc ttt atg gtt atg cac      144
Glu Asn Tyr Arg Gly Arg Asp Ala Thr Asp Val Phe Met Val Met His         
        35                  40                  45                      

tct gaa aat gct gtg agt aaa cta aga agg atg cct atc atg gaa cca      192
Ser Glu Asn Ala Val Ser Lys Leu Arg Arg Met Pro Ile Met Glu Pro         
    50                  55                  60                          

tca tct cca ctg acg cct acg cca ccg aaa ccc aac tca gac gaa ccg      240
Ser Ser Pro Leu Thr Pro Thr Pro Pro Lys Pro Asn Ser Asp Glu Pro         
65                  70                  75                  80          

cag gag gat ttc cgc aag ctc cga gat gag ctc atc gca gca gga atg      288
Gln Glu Asp Phe Arg Lys Leu Arg Asp Glu Leu Ile Ala Ala Gly Met         
                85                  90                  95              

ttc gac gca tca ccg atg tgg tac gca tat aag acg ctc agt acg ctg      336
Phe Asp Ala Ser Pro Met Trp Tyr Ala Tyr Lys Thr Leu Ser Thr Leu         
            100                 105                 110                 

ggc ctc ggg gtc ctc gcg gtg cta ttg atg acc cag tgg cac tgg tac      384
Gly Leu Gly Val Leu Ala Val Leu Leu Met Thr Gln Trp His Trp Tyr         
        115                 120                 125                     

ctc gtc ggg gca atc gtg ttg ggc att cac ttc caa caa atg ggt tgg      432
Leu Val Gly Ala Ile Val Leu Gly Ile His Phe Gln Gln Met Gly Trp         
    130                 135                 140                         

ttg tcg cac gat atc tgc cac cat cag ctg ttc aag gac cga tcg atc      480
Leu Ser His Asp Ile Cys His His Gln Leu Phe Lys Asp Arg Ser Ile         
145                 150                 155                 160         

aac aac gcc atc ggc ttg ctt ttc ggg aac gtc ttg caa ggg ttc tct      528
Asn Asn Ala Ile Gly Leu Leu Phe Gly Asn Val Leu Gln Gly Phe Ser         
                165                 170                 175             

gtg acc tgg tgg aag gac agg cac aat gca cac cac tcc gcc acc aac      576
Val Thr Trp Trp Lys Asp Arg His Asn Ala His His Ser Ala Thr Asn         
            180                 185                 190                 

gtg caa ggc cac gac ccc gac att gac aac ctg ccg ctg ctg gca tgg      624
Val Gln Gly His Asp Pro Asp Ile Asp Asn Leu Pro Leu Leu Ala Trp         
        195                 200                 205                     

tcc aag gag gac gtg gag agg gcc ggc ccg ttc tca cgg cgg atg atc      672
Ser Lys Glu Asp Val Glu Arg Ala Gly Pro Phe Ser Arg Arg Met Ile         
    210                 215                 220                         

aag tac cag caa tac tac ttc ttc ttc atc tgt gcc ctc ctg agg ttc      720
Lys Tyr Gln Gln Tyr Tyr Phe Phe Phe Ile Cys Ala Leu Leu Arg Phe         
225                 230                 235                 240         

atc tgg tgc ttc cag agc atc cac aca gcc aag ggc ctg aag gat cgc      768
Ile Trp Cys Phe Gln Ser Ile His Thr Ala Lys Gly Leu Lys Asp Arg         
                245                 250                 255             

agc aac cag tac tac cgc agg cag tac gag aaa gag agc gtg ggc ctg      816
Ser Asn Gln Tyr Tyr Arg Arg Gln Tyr Glu Lys Glu Ser Val Gly Leu         
            260                 265                 270                 

gcc ctc cac tgg ggc ctg aag gcg ttg ttc tac tac ttt tat atg cca      864
Ala Leu His Trp Gly Leu Lys Ala Leu Phe Tyr Tyr Phe Tyr Met Pro         
        275                 280                 285                     

agc ttc ttg acc gga ctc atg gtg ttt ttc gtg tcc gag ttg ctt ggg      912
Ser Phe Leu Thr Gly Leu Met Val Phe Phe Val Ser Glu Leu Leu Gly         
    290                 295                 300                         

ggc ttc ggc atc gcc atc gtg gtg ttc atg aac cac tac ccc ctg gag      960
Gly Phe Gly Ile Ala Ile Val Val Phe Met Asn His Tyr Pro Leu Glu         
305                 310                 315                 320         

aag atc cag gac tcg gtg tgg gac ggc cac ggc ttt tgc gcc ggc cag     1008
Lys Ile Gln Asp Ser Val Trp Asp Gly His Gly Phe Cys Ala Gly Gln         
                325                 330                 335             

att cac gaa acg atg aac gtc cag cgg gga ctc gtc acg gac tgg ttc     1056
Ile His Glu Thr Met Asn Val Gln Arg Gly Leu Val Thr Asp Trp Phe         
            340                 345                 350                 

ttc ggt ggg ctg aat tac caa atc gag cac cac ctg tgg ccg acg ctg     1104
Phe Gly Gly Leu Asn Tyr Gln Ile Glu His His Leu Trp Pro Thr Leu         
        355                 360                 365                     

ccc cgg cac aac ctg acg gcg gcc agc atc aaa gtg gag cag ttg tgc     1152
Pro Arg His Asn Leu Thr Ala Ala Ser Ile Lys Val Glu Gln Leu Cys         
    370                 375                 380                         

aag aag cac aac ttg ccg tat cgc agc ccc cca atg ctg gag ggg gtg     1200
Lys Lys His Asn Leu Pro Tyr Arg Ser Pro Pro Met Leu Glu Gly Val         
385                 390                 395                 400         

ggc atc ctg atc agc tac ctg ggc acc ttt gcc cgc atg gtg gca aag     1248
Gly Ile Leu Ile Ser Tyr Leu Gly Thr Phe Ala Arg Met Val Ala Lys         
                405                 410                 415             

gcc gac aag gcg                                                     1260
Ala Asp Lys Ala                                                         
            420                                                         


<210>  33
<211>  420
<212>  PRT
<213>  Euglena anabaena UTEX 373

<400>  33

Met Val Lys Arg Pro Ala Leu Pro Leu Thr Val Asp Gly Val Thr Tyr 
1               5                   10                  15      


Asp Val Ser Ala Trp Leu Asn His His Pro Gly Gly Ala Asp Ile Ile 
            20                  25                  30          


Glu Asn Tyr Arg Gly Arg Asp Ala Thr Asp Val Phe Met Val Met His 
        35                  40                  45              


Ser Glu Asn Ala Val Ser Lys Leu Arg Arg Met Pro Ile Met Glu Pro 
    50                  55                  60                  


Ser Ser Pro Leu Thr Pro Thr Pro Pro Lys Pro Asn Ser Asp Glu Pro 
65                  70                  75                  80  


Gln Glu Asp Phe Arg Lys Leu Arg Asp Glu Leu Ile Ala Ala Gly Met 
                85                  90                  95      


Phe Asp Ala Ser Pro Met Trp Tyr Ala Tyr Lys Thr Leu Ser Thr Leu 
            100                 105                 110         


Gly Leu Gly Val Leu Ala Val Leu Leu Met Thr Gln Trp His Trp Tyr 
        115                 120                 125             


Leu Val Gly Ala Ile Val Leu Gly Ile His Phe Gln Gln Met Gly Trp 
    130                 135                 140                 


Leu Ser His Asp Ile Cys His His Gln Leu Phe Lys Asp Arg Ser Ile 
145                 150                 155                 160 


Asn Asn Ala Ile Gly Leu Leu Phe Gly Asn Val Leu Gln Gly Phe Ser 
                165                 170                 175     


Val Thr Trp Trp Lys Asp Arg His Asn Ala His His Ser Ala Thr Asn 
            180                 185                 190         


Val Gln Gly His Asp Pro Asp Ile Asp Asn Leu Pro Leu Leu Ala Trp 
        195                 200                 205             


Ser Lys Glu Asp Val Glu Arg Ala Gly Pro Phe Ser Arg Arg Met Ile 
    210                 215                 220                 


Lys Tyr Gln Gln Tyr Tyr Phe Phe Phe Ile Cys Ala Leu Leu Arg Phe 
225                 230                 235                 240 


Ile Trp Cys Phe Gln Ser Ile His Thr Ala Lys Gly Leu Lys Asp Arg 
                245                 250                 255     


Ser Asn Gln Tyr Tyr Arg Arg Gln Tyr Glu Lys Glu Ser Val Gly Leu 
            260                 265                 270         


Ala Leu His Trp Gly Leu Lys Ala Leu Phe Tyr Tyr Phe Tyr Met Pro 
        275                 280                 285             


Ser Phe Leu Thr Gly Leu Met Val Phe Phe Val Ser Glu Leu Leu Gly 
    290                 295                 300                 


Gly Phe Gly Ile Ala Ile Val Val Phe Met Asn His Tyr Pro Leu Glu 
305                 310                 315                 320 


Lys Ile Gln Asp Ser Val Trp Asp Gly His Gly Phe Cys Ala Gly Gln 
                325                 330                 335     


Ile His Glu Thr Met Asn Val Gln Arg Gly Leu Val Thr Asp Trp Phe 
            340                 345                 350         


Phe Gly Gly Leu Asn Tyr Gln Ile Glu His His Leu Trp Pro Thr Leu 
        355                 360                 365             


Pro Arg His Asn Leu Thr Ala Ala Ser Ile Lys Val Glu Gln Leu Cys 
    370                 375                 380                 


Lys Lys His Asn Leu Pro Tyr Arg Ser Pro Pro Met Leu Glu Gly Val 
385                 390                 395                 400 


Gly Ile Leu Ile Ser Tyr Leu Gly Thr Phe Ala Arg Met Val Ala Lys 
                405                 410                 415     


Ala Asp Lys Ala 
            420 


<210>  34
<211>  1350
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(1350)
<223>  delta-5 desaturase

<300>
<302>  DELTA-5 DESATURASE AND ITS USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  WO 2007/136671
<311>  2007-05-16
<312>  2007-11-29
<313>  (1)..(1350)

<300>
<302>  DELTA-5 DESATURASE AND ITS USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  US 2007-0292924-A1
<311>  2007-05-15
<312>  2007-12-20
<313>  (1)..(1350)

<400>  34
atg gct ctc agt ctt acc aca gaa cag ctg tta gaa cgc cct gat ttg       48
Met Ala Leu Ser Leu Thr Thr Glu Gln Leu Leu Glu Arg Pro Asp Leu         
1               5                   10                  15              

gtt gcg att gat ggc atc ctc tac gac ctt gaa ggg ctt gcc aaa gtt       96
Val Ala Ile Asp Gly Ile Leu Tyr Asp Leu Glu Gly Leu Ala Lys Val         
            20                  25                  30                  

cat cca gga gga gat ttg att ctc gct tct ggt gcc tct gat gcc tcc      144
His Pro Gly Gly Asp Leu Ile Leu Ala Ser Gly Ala Ser Asp Ala Ser         
        35                  40                  45                      

cct ctc ttt tat tca atg cat cca tac gtc aaa ccg gag aat tcc aaa      192
Pro Leu Phe Tyr Ser Met His Pro Tyr Val Lys Pro Glu Asn Ser Lys         
    50                  55                  60                          

ttg ctt caa cag ttc gtc cga ggg aag cat gac cgc acc tcg aag gac      240
Leu Leu Gln Gln Phe Val Arg Gly Lys His Asp Arg Thr Ser Lys Asp         
65                  70                  75                  80          

att gtc tac acg tat gat tct ccc ttc gca caa gac gtt aag cgg aca      288
Ile Val Tyr Thr Tyr Asp Ser Pro Phe Ala Gln Asp Val Lys Arg Thr         
                85                  90                  95              

atg cgc gag gtg atg aaa ggg agg aac tgg tac gca acc cct ggc ttc      336
Met Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly Phe         
            100                 105                 110                 

tgg ctg cgc acc gtt ggg atc atc gcc gtg acg gcc ttt tgc gag tgg      384
Trp Leu Arg Thr Val Gly Ile Ile Ala Val Thr Ala Phe Cys Glu Trp         
        115                 120                 125                     

cac tgg gct acc acg ggg atg gtg ctg tgg ggc ctg ttg act gga ttc      432
His Trp Ala Thr Thr Gly Met Val Leu Trp Gly Leu Leu Thr Gly Phe         
    130                 135                 140                         

atg cac atg cag atc ggc tta tcc atc cag cat gat gcg tcc cac ggg      480
Met His Met Gln Ile Gly Leu Ser Ile Gln His Asp Ala Ser His Gly         
145                 150                 155                 160         

gcc atc agc aag aag cct tgg gtc aac gcc ctc ttc gcc tac ggc att      528
Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Leu Phe Ala Tyr Gly Ile         
                165                 170                 175             

gac gtc atc gga tcg tcc cgg tgg att tgg ctg cag tcg cac atc atg      576
Asp Val Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile Met         
            180                 185                 190                 

cgg cac cac acc tac acc aac cag cac ggc ctc gac ctg gat gcg gag      624
Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala Glu         
        195                 200                 205                     

tcg gca gag ccg ttc ctg gtg ttc cac aac tac ccc gcc gca aac acc      672
Ser Ala Glu Pro Phe Leu Val Phe His Asn Tyr Pro Ala Ala Asn Thr         
    210                 215                 220                         

gcc cga aag tgg ttc cac cgc ttc caa gct tgg tac atg tac ctt gtg      720
Ala Arg Lys Trp Phe His Arg Phe Gln Ala Trp Tyr Met Tyr Leu Val         
225                 230                 235                 240         

ctg ggg gca tac ggg gta tcg ctg gtg tac aac ccg ctc tac att ttc      768
Leu Gly Ala Tyr Gly Val Ser Leu Val Tyr Asn Pro Leu Tyr Ile Phe         
                245                 250                 255             

cgg atg cag cac aat gac acc atc cca gag tct gtc acg gcc atg cgg      816
Arg Met Gln His Asn Asp Thr Ile Pro Glu Ser Val Thr Ala Met Arg         
            260                 265                 270                 

gag aat ggc ttt ctg cgg cgc tac cgc aca ctt gca ttc gtg atg cga      864
Glu Asn Gly Phe Leu Arg Arg Tyr Arg Thr Leu Ala Phe Val Met Arg         
        275                 280                 285                     

gct ttc ttc atc ttc cgg acc gca ttc ttg ccc tgg tac ctc act ggg      912
Ala Phe Phe Ile Phe Arg Thr Ala Phe Leu Pro Trp Tyr Leu Thr Gly         
    290                 295                 300                         

acc tca ttg ctg atc acc att cct ctg gtg ccc act gca act ggt gcc      960
Thr Ser Leu Leu Ile Thr Ile Pro Leu Val Pro Thr Ala Thr Gly Ala         
305                 310                 315                 320         

ttc ttg acg ttc ttc ttc att ttg tcc cac aat ttt gat ggc tcc gaa     1008
Phe Leu Thr Phe Phe Phe Ile Leu Ser His Asn Phe Asp Gly Ser Glu         
                325                 330                 335             

cgg atc ccc gac aag aac tgc aag gtt aag agc tct gag aag gac gtt     1056
Arg Ile Pro Asp Lys Asn Cys Lys Val Lys Ser Ser Glu Lys Asp Val         
            340                 345                 350                 

gag gct gac caa att gac tgg tat cgg gcg cag gtg gag acg tcc tcc     1104
Glu Ala Asp Gln Ile Asp Trp Tyr Arg Ala Gln Val Glu Thr Ser Ser         
        355                 360                 365                     

aca tac ggt ggc ccc atc gcc atg ttc ttc act ggc ggt ctc aat ttc     1152
Thr Tyr Gly Gly Pro Ile Ala Met Phe Phe Thr Gly Gly Leu Asn Phe         
    370                 375                 380                         

cag atc gag cac cac ctc ttt ccc cgg atg tcg tct tgg cac tac ccc     1200
Gln Ile Glu His His Leu Phe Pro Arg Met Ser Ser Trp His Tyr Pro         
385                 390                 395                 400         

ttc gtc cag cag gcg gtc cgg gag tgt tgc gaa cgc cat gga gtg cga     1248
Phe Val Gln Gln Ala Val Arg Glu Cys Cys Glu Arg His Gly Val Arg         
                405                 410                 415             

tat gtt ttc tac cct acc atc gtc ggc aac atc atc tcc acc ctg aag     1296
Tyr Val Phe Tyr Pro Thr Ile Val Gly Asn Ile Ile Ser Thr Leu Lys         
            420                 425                 430                 

tac atg cat aag gtg ggt gtc gtc cac tgc gtg aag gac gca cag gat     1344
Tyr Met His Lys Val Gly Val Val His Cys Val Lys Asp Ala Gln Asp         
        435                 440                 445                     

tcc tga                                                             1350
Ser                                                                     
                                                                        


<210>  35
<211>  449
<212>  PRT
<213>  Euglena gracilis

<400>  35

Met Ala Leu Ser Leu Thr Thr Glu Gln Leu Leu Glu Arg Pro Asp Leu 
1               5                   10                  15      


Val Ala Ile Asp Gly Ile Leu Tyr Asp Leu Glu Gly Leu Ala Lys Val 
            20                  25                  30          


His Pro Gly Gly Asp Leu Ile Leu Ala Ser Gly Ala Ser Asp Ala Ser 
        35                  40                  45              


Pro Leu Phe Tyr Ser Met His Pro Tyr Val Lys Pro Glu Asn Ser Lys 
    50                  55                  60                  


Leu Leu Gln Gln Phe Val Arg Gly Lys His Asp Arg Thr Ser Lys Asp 
65                  70                  75                  80  


Ile Val Tyr Thr Tyr Asp Ser Pro Phe Ala Gln Asp Val Lys Arg Thr 
                85                  90                  95      


Met Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly Phe 
            100                 105                 110         


Trp Leu Arg Thr Val Gly Ile Ile Ala Val Thr Ala Phe Cys Glu Trp 
        115                 120                 125             


His Trp Ala Thr Thr Gly Met Val Leu Trp Gly Leu Leu Thr Gly Phe 
    130                 135                 140                 


Met His Met Gln Ile Gly Leu Ser Ile Gln His Asp Ala Ser His Gly 
145                 150                 155                 160 


Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Leu Phe Ala Tyr Gly Ile 
                165                 170                 175     


Asp Val Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile Met 
            180                 185                 190         


Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala Glu 
        195                 200                 205             


Ser Ala Glu Pro Phe Leu Val Phe His Asn Tyr Pro Ala Ala Asn Thr 
    210                 215                 220                 


Ala Arg Lys Trp Phe His Arg Phe Gln Ala Trp Tyr Met Tyr Leu Val 
225                 230                 235                 240 


Leu Gly Ala Tyr Gly Val Ser Leu Val Tyr Asn Pro Leu Tyr Ile Phe 
                245                 250                 255     


Arg Met Gln His Asn Asp Thr Ile Pro Glu Ser Val Thr Ala Met Arg 
            260                 265                 270         


Glu Asn Gly Phe Leu Arg Arg Tyr Arg Thr Leu Ala Phe Val Met Arg 
        275                 280                 285             


Ala Phe Phe Ile Phe Arg Thr Ala Phe Leu Pro Trp Tyr Leu Thr Gly 
    290                 295                 300                 


Thr Ser Leu Leu Ile Thr Ile Pro Leu Val Pro Thr Ala Thr Gly Ala 
305                 310                 315                 320 


Phe Leu Thr Phe Phe Phe Ile Leu Ser His Asn Phe Asp Gly Ser Glu 
                325                 330                 335     


Arg Ile Pro Asp Lys Asn Cys Lys Val Lys Ser Ser Glu Lys Asp Val 
            340                 345                 350         


Glu Ala Asp Gln Ile Asp Trp Tyr Arg Ala Gln Val Glu Thr Ser Ser 
        355                 360                 365             


Thr Tyr Gly Gly Pro Ile Ala Met Phe Phe Thr Gly Gly Leu Asn Phe 
    370                 375                 380                 


Gln Ile Glu His His Leu Phe Pro Arg Met Ser Ser Trp His Tyr Pro 
385                 390                 395                 400 


Phe Val Gln Gln Ala Val Arg Glu Cys Cys Glu Arg His Gly Val Arg 
                405                 410                 415     


Tyr Val Phe Tyr Pro Thr Ile Val Gly Asn Ile Ile Ser Thr Leu Lys 
            420                 425                 430         


Tyr Met His Lys Val Gly Val Val His Cys Val Lys Asp Ala Gln Asp 
        435                 440                 445             


Ser 
    


<210>  36
<211>  1350
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(1350)
<223>  synthetic delta-5 desaturase (codon-optimized for Yarrowia 
       lipolytica)

<300>
<302>  DELTA-5 DESATURASE AND ITS USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  WO 2007/136671
<311>  2007-05-16
<312>  2007-11-29
<313>  (1)..(1350)

<300>
<302>  DELTA-5 DESATURASE AND ITS USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  US 2007-0292924-A1
<311>  2007-05-15
<312>  2007-12-20
<313>  (1)..(1350)

<400>  36
atg gct ctc tcc ctt act acc gag cag ctg ctc gag cga ccc gac ctg       48
Met Ala Leu Ser Leu Thr Thr Glu Gln Leu Leu Glu Arg Pro Asp Leu         
1               5                   10                  15              

gtt gcc atc gac ggc att ctc tac gat ctg gaa ggt ctt gcc aag gtc       96
Val Ala Ile Asp Gly Ile Leu Tyr Asp Leu Glu Gly Leu Ala Lys Val         
            20                  25                  30                  

cat ccc gga ggc gac ttg atc ctc gct tct ggt gcc tcc gat gct tct      144
His Pro Gly Gly Asp Leu Ile Leu Ala Ser Gly Ala Ser Asp Ala Ser         
        35                  40                  45                      

cct ctg ttc tac tcc atg cac cct tac gtc aag ccc gag aac tcg aag      192
Pro Leu Phe Tyr Ser Met His Pro Tyr Val Lys Pro Glu Asn Ser Lys         
    50                  55                  60                          

ctg ctt caa cag ttc gtg cga ggc aag cac gac cga acc tcc aag gac      240
Leu Leu Gln Gln Phe Val Arg Gly Lys His Asp Arg Thr Ser Lys Asp         
65                  70                  75                  80          

att gtc tac acc tac gac tct ccc ttt gca cag gac gtc aag cga act      288
Ile Val Tyr Thr Tyr Asp Ser Pro Phe Ala Gln Asp Val Lys Arg Thr         
                85                  90                  95              

atg cga gag gtc atg aaa ggt cgg aac tgg tat gcc aca cct gga ttc      336
Met Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly Phe         
            100                 105                 110                 

tgg ctg cga acc gtt ggc atc att gct gtc acc gcc ttt tgc gag tgg      384
Trp Leu Arg Thr Val Gly Ile Ile Ala Val Thr Ala Phe Cys Glu Trp         
        115                 120                 125                     

cac tgg gct act acc gga atg gtg ctg tgg ggt ctc ttg act gga ttc      432
His Trp Ala Thr Thr Gly Met Val Leu Trp Gly Leu Leu Thr Gly Phe         
    130                 135                 140                         

atg cac atg cag atc ggc ctg tcc att cag cac gat gcc tct cat ggt      480
Met His Met Gln Ile Gly Leu Ser Ile Gln His Asp Ala Ser His Gly         
145                 150                 155                 160         

gcc atc agc aaa aag ccc tgg gtc aac gct ctc ttt gcc tac ggc atc      528
Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Leu Phe Ala Tyr Gly Ile         
                165                 170                 175             

gac gtc att gga tcg tcc aga tgg atc tgg ctg cag tct cac atc atg      576
Asp Val Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile Met         
            180                 185                 190                 

cga cat cac acc tac acc aat cag cat ggt ctc gac ctg gat gcc gag      624
Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala Glu         
        195                 200                 205                     

tcc gca gaa cca ttc ctt gtg ttc cac aac tac cct gct gcc aac act      672
Ser Ala Glu Pro Phe Leu Val Phe His Asn Tyr Pro Ala Ala Asn Thr         
    210                 215                 220                         

gct cga aag tgg ttt cac cga ttc cag gcc tgg tac atg tac ctc gtg      720
Ala Arg Lys Trp Phe His Arg Phe Gln Ala Trp Tyr Met Tyr Leu Val         
225                 230                 235                 240         

ctt gga gcc tac ggc gtt tcg ctg gtg tac aac cct ctc tac atc ttc      768
Leu Gly Ala Tyr Gly Val Ser Leu Val Tyr Asn Pro Leu Tyr Ile Phe         
                245                 250                 255             

cga atg cag cac aac gac acc att ccc gag tct gtc aca gcc atg cga      816
Arg Met Gln His Asn Asp Thr Ile Pro Glu Ser Val Thr Ala Met Arg         
            260                 265                 270                 

gag aac ggc ttt ctg cga cgg tac cga acc ctt gca ttc gtt atg cga      864
Glu Asn Gly Phe Leu Arg Arg Tyr Arg Thr Leu Ala Phe Val Met Arg         
        275                 280                 285                     

gct ttc ttc atc ttt cga acc gcc ttc ttg ccc tgg tat ctc act gga      912
Ala Phe Phe Ile Phe Arg Thr Ala Phe Leu Pro Trp Tyr Leu Thr Gly         
    290                 295                 300                         

acc tcc ctg ctc atc acc att cct ctg gtg ccc act gct acc ggt gcc      960
Thr Ser Leu Leu Ile Thr Ile Pro Leu Val Pro Thr Ala Thr Gly Ala         
305                 310                 315                 320         

ttc ctc acc ttc ttt ttc atc ttg tct cac aac ttc gat ggc tcg gag     1008
Phe Leu Thr Phe Phe Phe Ile Leu Ser His Asn Phe Asp Gly Ser Glu         
                325                 330                 335             

cga atc ccc gac aag aac tgc aag gtc aag agc tcc gag aag gac gtt     1056
Arg Ile Pro Asp Lys Asn Cys Lys Val Lys Ser Ser Glu Lys Asp Val         
            340                 345                 350                 

gaa gcc gat cag atc gac tgg tac aga gct cag gtg gag acc tct tcc     1104
Glu Ala Asp Gln Ile Asp Trp Tyr Arg Ala Gln Val Glu Thr Ser Ser         
        355                 360                 365                     

acc tac ggt gga ccc att gcc atg ttc ttt act ggc ggt ctc aac ttc     1152
Thr Tyr Gly Gly Pro Ile Ala Met Phe Phe Thr Gly Gly Leu Asn Phe         
    370                 375                 380                         

cag atc gag cat cac ctc ttt cct cga atg tcg tct tgg cac tat ccc     1200
Gln Ile Glu His His Leu Phe Pro Arg Met Ser Ser Trp His Tyr Pro         
385                 390                 395                 400         

ttc gtg cag caa gct gtc cga gag tgt tgc gaa cga cac gga gtt cgg     1248
Phe Val Gln Gln Ala Val Arg Glu Cys Cys Glu Arg His Gly Val Arg         
                405                 410                 415             

tac gtc ttc tac cct acc att gtg ggc aac atc att tcc acc ctc aag     1296
Tyr Val Phe Tyr Pro Thr Ile Val Gly Asn Ile Ile Ser Thr Leu Lys         
            420                 425                 430                 

tac atg cac aaa gtc ggt gtg gtt cac tgt gtc aag gac gct cag gat     1344
Tyr Met His Lys Val Gly Val Val His Cys Val Lys Asp Ala Gln Asp         
        435                 440                 445                     

tcc taa                                                             1350
Ser                                                                     
                                                                        


<210>  37
<211>  449
<212>  PRT
<213>  Euglena gracilis

<400>  37

Met Ala Leu Ser Leu Thr Thr Glu Gln Leu Leu Glu Arg Pro Asp Leu 
1               5                   10                  15      


Val Ala Ile Asp Gly Ile Leu Tyr Asp Leu Glu Gly Leu Ala Lys Val 
            20                  25                  30          


His Pro Gly Gly Asp Leu Ile Leu Ala Ser Gly Ala Ser Asp Ala Ser 
        35                  40                  45              


Pro Leu Phe Tyr Ser Met His Pro Tyr Val Lys Pro Glu Asn Ser Lys 
    50                  55                  60                  


Leu Leu Gln Gln Phe Val Arg Gly Lys His Asp Arg Thr Ser Lys Asp 
65                  70                  75                  80  


Ile Val Tyr Thr Tyr Asp Ser Pro Phe Ala Gln Asp Val Lys Arg Thr 
                85                  90                  95      


Met Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly Phe 
            100                 105                 110         


Trp Leu Arg Thr Val Gly Ile Ile Ala Val Thr Ala Phe Cys Glu Trp 
        115                 120                 125             


His Trp Ala Thr Thr Gly Met Val Leu Trp Gly Leu Leu Thr Gly Phe 
    130                 135                 140                 


Met His Met Gln Ile Gly Leu Ser Ile Gln His Asp Ala Ser His Gly 
145                 150                 155                 160 


Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Leu Phe Ala Tyr Gly Ile 
                165                 170                 175     


Asp Val Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile Met 
            180                 185                 190         


Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala Glu 
        195                 200                 205             


Ser Ala Glu Pro Phe Leu Val Phe His Asn Tyr Pro Ala Ala Asn Thr 
    210                 215                 220                 


Ala Arg Lys Trp Phe His Arg Phe Gln Ala Trp Tyr Met Tyr Leu Val 
225                 230                 235                 240 


Leu Gly Ala Tyr Gly Val Ser Leu Val Tyr Asn Pro Leu Tyr Ile Phe 
                245                 250                 255     


Arg Met Gln His Asn Asp Thr Ile Pro Glu Ser Val Thr Ala Met Arg 
            260                 265                 270         


Glu Asn Gly Phe Leu Arg Arg Tyr Arg Thr Leu Ala Phe Val Met Arg 
        275                 280                 285             


Ala Phe Phe Ile Phe Arg Thr Ala Phe Leu Pro Trp Tyr Leu Thr Gly 
    290                 295                 300                 


Thr Ser Leu Leu Ile Thr Ile Pro Leu Val Pro Thr Ala Thr Gly Ala 
305                 310                 315                 320 


Phe Leu Thr Phe Phe Phe Ile Leu Ser His Asn Phe Asp Gly Ser Glu 
                325                 330                 335     


Arg Ile Pro Asp Lys Asn Cys Lys Val Lys Ser Ser Glu Lys Asp Val 
            340                 345                 350         


Glu Ala Asp Gln Ile Asp Trp Tyr Arg Ala Gln Val Glu Thr Ser Ser 
        355                 360                 365             


Thr Tyr Gly Gly Pro Ile Ala Met Phe Phe Thr Gly Gly Leu Asn Phe 
    370                 375                 380                 


Gln Ile Glu His His Leu Phe Pro Arg Met Ser Ser Trp His Tyr Pro 
385                 390                 395                 400 


Phe Val Gln Gln Ala Val Arg Glu Cys Cys Glu Arg His Gly Val Arg 
                405                 410                 415     


Tyr Val Phe Tyr Pro Thr Ile Val Gly Asn Ile Ile Ser Thr Leu Lys 
            420                 425                 430         


Tyr Met His Lys Val Gly Val Val His Cys Val Lys Asp Ala Gln Asp 
        435                 440                 445             


Ser 
    


<210>  38
<211>  1392
<212>  DNA
<213>  Peridinium sp. CCMP626


<220>
<221>  CDS
<222>  (1)..(1392)
<223>  delta-5 desaturase

<300>
<302>  DELTA-5 DESATURASE AND ITS USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  WO 2007/136646
<311>  2007-05-16
<312>  2007-11-29
<313>  (1)..(1392)

<300>
<302>  DELTA-5 DESATURASE AND ITS USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  US 2007-0271632-A1
<311>  2007-05-15
<312>  2007-11-22
<313>  (1)..(1392)

<400>  38
atg gct cca gat gcg gac aag ttg aga cag cgc aag gcg caa tcg att       48
Met Ala Pro Asp Ala Asp Lys Leu Arg Gln Arg Lys Ala Gln Ser Ile         
1               5                   10                  15              

caa gac acg gct gat tcg caa gct acc gaa ctc aag att ggc acc ctg       96
Gln Asp Thr Ala Asp Ser Gln Ala Thr Glu Leu Lys Ile Gly Thr Leu         
            20                  25                  30                  

aag ggc ttg cag ggg aca gaa atc gtc att gat gga gac att tac gat      144
Lys Gly Leu Gln Gly Thr Glu Ile Val Ile Asp Gly Asp Ile Tyr Asp         
        35                  40                  45                      

ata aaa gac ttt gat cac ccc ggt ggt gaa tcc atc atg act ttt ggg      192
Ile Lys Asp Phe Asp His Pro Gly Gly Glu Ser Ile Met Thr Phe Gly         
    50                  55                  60                          

gga aac gat gtc acc gcc acg tac aag atg atc cac ccc tac cac tct      240
Gly Asn Asp Val Thr Ala Thr Tyr Lys Met Ile His Pro Tyr His Ser         
65                  70                  75                  80          

aag cac cat ttg gag aag atg aag aaa gtg gga cga gtt ccg gac tac      288
Lys His His Leu Glu Lys Met Lys Lys Val Gly Arg Val Pro Asp Tyr         
                85                  90                  95              

acc tcg gaa tac aag ttt gat act ccc ttt gag cgt gaa atc aag caa      336
Thr Ser Glu Tyr Lys Phe Asp Thr Pro Phe Glu Arg Glu Ile Lys Gln         
            100                 105                 110                 

gag gtc ttc aag att gtg cga cga ggc cgc gag ttt gga aca cct gga      384
Glu Val Phe Lys Ile Val Arg Arg Gly Arg Glu Phe Gly Thr Pro Gly         
        115                 120                 125                     

tac ttc ttc cgg gct ttc tgc tac att gga ctt ttc ttt tac ttg cag      432
Tyr Phe Phe Arg Ala Phe Cys Tyr Ile Gly Leu Phe Phe Tyr Leu Gln         
    130                 135                 140                         

tat ttg tgg gtc acg act ccc act acc ttt gcc ttg gcg atc ttc tat      480
Tyr Leu Trp Val Thr Thr Pro Thr Thr Phe Ala Leu Ala Ile Phe Tyr         
145                 150                 155                 160         

ggt gtt tcg caa gct ttc att ggt ttg aac gta caa cat gat gcc aac      528
Gly Val Ser Gln Ala Phe Ile Gly Leu Asn Val Gln His Asp Ala Asn         
                165                 170                 175             

cac gga gct gcc tcc aag aag cct tgg atc aat aac ttg cta gga ttg      576
His Gly Ala Ala Ser Lys Lys Pro Trp Ile Asn Asn Leu Leu Gly Leu         
            180                 185                 190                 

ggg gct gac ttt atc gga ggt tcc aaa tgg ttg tgg atg aac cag cac      624
Gly Ala Asp Phe Ile Gly Gly Ser Lys Trp Leu Trp Met Asn Gln His         
        195                 200                 205                     

tgg acg cac cac aca tac acc aac cac cat gag aag gat ccc gat gcc      672
Trp Thr His His Thr Tyr Thr Asn His His Glu Lys Asp Pro Asp Ala         
    210                 215                 220                         

ttg ggc gct gaa cca atg ttg ttg ttc aat gat tat ccc ttg ggt cac      720
Leu Gly Ala Glu Pro Met Leu Leu Phe Asn Asp Tyr Pro Leu Gly His         
225                 230                 235                 240         

cca aag cgt act ttg att cac cac ttc cag gcc ttc tat tac ctt ttc      768
Pro Lys Arg Thr Leu Ile His His Phe Gln Ala Phe Tyr Tyr Leu Phe         
                245                 250                 255             

gtc ttg gcc gga tac tgg gtc tct tcg gtc ttc aac cct caa att ttg      816
Val Leu Ala Gly Tyr Trp Val Ser Ser Val Phe Asn Pro Gln Ile Leu         
            260                 265                 270                 

gac ttg caa cac cgc ggt gct caa gcg gtt gga atg aaa atg gag aac      864
Asp Leu Gln His Arg Gly Ala Gln Ala Val Gly Met Lys Met Glu Asn         
        275                 280                 285                     

gat tac att gcc aaa agc cga aag tat gcc atc ttc ttg cgt ctc ttg      912
Asp Tyr Ile Ala Lys Ser Arg Lys Tyr Ala Ile Phe Leu Arg Leu Leu         
    290                 295                 300                         

tat att tac acc aac att gtc gct ccg atc caa aac caa ggc ttc tcg      960
Tyr Ile Tyr Thr Asn Ile Val Ala Pro Ile Gln Asn Gln Gly Phe Ser         
305                 310                 315                 320         

ttg acc gtg gtc gcc cac att ttg acc atg ggc gtc gct tcc agt ttg     1008
Leu Thr Val Val Ala His Ile Leu Thr Met Gly Val Ala Ser Ser Leu         
                325                 330                 335             

act ttg gcg act ctt ttt gcc ttg tcg cac aat ttt gaa aac gcg gat     1056
Thr Leu Ala Thr Leu Phe Ala Leu Ser His Asn Phe Glu Asn Ala Asp         
            340                 345                 350                 

cgc gat ccc act tac gag gcc cgc aag gga gga gag cct gtt tgt tgg     1104
Arg Asp Pro Thr Tyr Glu Ala Arg Lys Gly Gly Glu Pro Val Cys Trp         
        355                 360                 365                     

ttc aag tcg caa gtc gaa acc tcg tca act tac gga ggt ttc atc tcg     1152
Phe Lys Ser Gln Val Glu Thr Ser Ser Thr Tyr Gly Gly Phe Ile Ser         
    370                 375                 380                         

ggt tgc ttg acg ggc gga ctc aac ttc caa gtg gaa cac cac ttg ttc     1200
Gly Cys Leu Thr Gly Gly Leu Asn Phe Gln Val Glu His His Leu Phe         
385                 390                 395                 400         

cct cgt atg agt tcg gcc tgg tac ccc tac att gcc cct act gtt cga     1248
Pro Arg Met Ser Ser Ala Trp Tyr Pro Tyr Ile Ala Pro Thr Val Arg         
                405                 410                 415             

gag gtt tgc aaa aag cac gga gtc aag tac gca tac tat ccc tgg gtc     1296
Glu Val Cys Lys Lys His Gly Val Lys Tyr Ala Tyr Tyr Pro Trp Val         
            420                 425                 430                 

tgg caa aac ttg att tca act gtc aag tat ctg cat caa agc gga act     1344
Trp Gln Asn Leu Ile Ser Thr Val Lys Tyr Leu His Gln Ser Gly Thr         
        435                 440                 445                     

gga tcc aac tgg aag aat ggc gcc aac ccc tac tcg gga aaa ttg taa     1392
Gly Ser Asn Trp Lys Asn Gly Ala Asn Pro Tyr Ser Gly Lys Leu             
    450                 455                 460                         


<210>  39
<211>  463
<212>  PRT
<213>  Peridinium sp. CCMP626

<400>  39

Met Ala Pro Asp Ala Asp Lys Leu Arg Gln Arg Lys Ala Gln Ser Ile 
1               5                   10                  15      


Gln Asp Thr Ala Asp Ser Gln Ala Thr Glu Leu Lys Ile Gly Thr Leu 
            20                  25                  30          


Lys Gly Leu Gln Gly Thr Glu Ile Val Ile Asp Gly Asp Ile Tyr Asp 
        35                  40                  45              


Ile Lys Asp Phe Asp His Pro Gly Gly Glu Ser Ile Met Thr Phe Gly 
    50                  55                  60                  


Gly Asn Asp Val Thr Ala Thr Tyr Lys Met Ile His Pro Tyr His Ser 
65                  70                  75                  80  


Lys His His Leu Glu Lys Met Lys Lys Val Gly Arg Val Pro Asp Tyr 
                85                  90                  95      


Thr Ser Glu Tyr Lys Phe Asp Thr Pro Phe Glu Arg Glu Ile Lys Gln 
            100                 105                 110         


Glu Val Phe Lys Ile Val Arg Arg Gly Arg Glu Phe Gly Thr Pro Gly 
        115                 120                 125             


Tyr Phe Phe Arg Ala Phe Cys Tyr Ile Gly Leu Phe Phe Tyr Leu Gln 
    130                 135                 140                 


Tyr Leu Trp Val Thr Thr Pro Thr Thr Phe Ala Leu Ala Ile Phe Tyr 
145                 150                 155                 160 


Gly Val Ser Gln Ala Phe Ile Gly Leu Asn Val Gln His Asp Ala Asn 
                165                 170                 175     


His Gly Ala Ala Ser Lys Lys Pro Trp Ile Asn Asn Leu Leu Gly Leu 
            180                 185                 190         


Gly Ala Asp Phe Ile Gly Gly Ser Lys Trp Leu Trp Met Asn Gln His 
        195                 200                 205             


Trp Thr His His Thr Tyr Thr Asn His His Glu Lys Asp Pro Asp Ala 
    210                 215                 220                 


Leu Gly Ala Glu Pro Met Leu Leu Phe Asn Asp Tyr Pro Leu Gly His 
225                 230                 235                 240 


Pro Lys Arg Thr Leu Ile His His Phe Gln Ala Phe Tyr Tyr Leu Phe 
                245                 250                 255     


Val Leu Ala Gly Tyr Trp Val Ser Ser Val Phe Asn Pro Gln Ile Leu 
            260                 265                 270         


Asp Leu Gln His Arg Gly Ala Gln Ala Val Gly Met Lys Met Glu Asn 
        275                 280                 285             


Asp Tyr Ile Ala Lys Ser Arg Lys Tyr Ala Ile Phe Leu Arg Leu Leu 
    290                 295                 300                 


Tyr Ile Tyr Thr Asn Ile Val Ala Pro Ile Gln Asn Gln Gly Phe Ser 
305                 310                 315                 320 


Leu Thr Val Val Ala His Ile Leu Thr Met Gly Val Ala Ser Ser Leu 
                325                 330                 335     


Thr Leu Ala Thr Leu Phe Ala Leu Ser His Asn Phe Glu Asn Ala Asp 
            340                 345                 350         


Arg Asp Pro Thr Tyr Glu Ala Arg Lys Gly Gly Glu Pro Val Cys Trp 
        355                 360                 365             


Phe Lys Ser Gln Val Glu Thr Ser Ser Thr Tyr Gly Gly Phe Ile Ser 
    370                 375                 380                 


Gly Cys Leu Thr Gly Gly Leu Asn Phe Gln Val Glu His His Leu Phe 
385                 390                 395                 400 


Pro Arg Met Ser Ser Ala Trp Tyr Pro Tyr Ile Ala Pro Thr Val Arg 
                405                 410                 415     


Glu Val Cys Lys Lys His Gly Val Lys Tyr Ala Tyr Tyr Pro Trp Val 
            420                 425                 430         


Trp Gln Asn Leu Ile Ser Thr Val Lys Tyr Leu His Gln Ser Gly Thr 
        435                 440                 445             


Gly Ser Asn Trp Lys Asn Gly Ala Asn Pro Tyr Ser Gly Lys Leu 
    450                 455                 460             


<210>  40
<211>  1392
<212>  DNA
<213>  Peridinium sp. CCMP626


<220>
<221>  CDS
<222>  (1)..(1392)
<223>  synthetic delta-5 desaturase (codon-optimized for Yarrowia 
       lipolytica)

<300>
<302>  DELTA-5 DESATURASE AND ITS USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  WO 2007/136646
<311>  2007-05-16
<312>  2007-11-29
<313>  (1)..(1392)

<300>
<302>  DELTA-5 DESATURASE AND ITS USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  US 2007-0271632-A1
<311>  2007-05-15
<312>  2007-11-22
<313>  (1)..(1392)

<400>  40
atg gct ccc gac gcc gac aag ctg cga cag cga aag gct cag tcc atc       48
Met Ala Pro Asp Ala Asp Lys Leu Arg Gln Arg Lys Ala Gln Ser Ile         
1               5                   10                  15              

cag gac act gcc gat tct cag gct acc gag ctc aag att ggc acc ctg       96
Gln Asp Thr Ala Asp Ser Gln Ala Thr Glu Leu Lys Ile Gly Thr Leu         
            20                  25                  30                  

aag ggt ctc caa ggc acc gag atc gtc att gat ggc gac atc tac gac      144
Lys Gly Leu Gln Gly Thr Glu Ile Val Ile Asp Gly Asp Ile Tyr Asp         
        35                  40                  45                      

atc aaa gac ttc gat cac cct gga ggc gaa tcc atc atg acc ttt ggt      192
Ile Lys Asp Phe Asp His Pro Gly Gly Glu Ser Ile Met Thr Phe Gly         
    50                  55                  60                          

ggc aac gac gtt act gcc acc tac aag atg att cat ccc tac cac tcg      240
Gly Asn Asp Val Thr Ala Thr Tyr Lys Met Ile His Pro Tyr His Ser         
65                  70                  75                  80          

aag cat cac ctg gag aag atg aaa aag gtc ggt cga gtg ccc gac tac      288
Lys His His Leu Glu Lys Met Lys Lys Val Gly Arg Val Pro Asp Tyr         
                85                  90                  95              

acc tcc gag tac aag ttc gat act ccc ttc gaa cga gag atc aaa cag      336
Thr Ser Glu Tyr Lys Phe Asp Thr Pro Phe Glu Arg Glu Ile Lys Gln         
            100                 105                 110                 

gag gtc ttc aag att gtg cga aga ggt cga gag ttt gga aca cct ggc      384
Glu Val Phe Lys Ile Val Arg Arg Gly Arg Glu Phe Gly Thr Pro Gly         
        115                 120                 125                     

tac ttc ttt cga gcc ttc tgc tac atc ggt ctc ttc ttt tac ctg cag      432
Tyr Phe Phe Arg Ala Phe Cys Tyr Ile Gly Leu Phe Phe Tyr Leu Gln         
    130                 135                 140                         

tat ctc tgg gtt acc act cct acc act ttc gcc ctt gct atc ttc tac      480
Tyr Leu Trp Val Thr Thr Pro Thr Thr Phe Ala Leu Ala Ile Phe Tyr         
145                 150                 155                 160         

ggt gtg tct cag gcc ttc att ggc ctg aac gtc cag cac gac gcc aac      528
Gly Val Ser Gln Ala Phe Ile Gly Leu Asn Val Gln His Asp Ala Asn         
                165                 170                 175             

cac gga gct gcc tcc aaa aag ccc tgg atc aac aat ttg ctc ggc ctg      576
His Gly Ala Ala Ser Lys Lys Pro Trp Ile Asn Asn Leu Leu Gly Leu         
            180                 185                 190                 

ggt gcc gac ttt atc gga ggc tcc aag tgg ctc tgg atg aac cag cac      624
Gly Ala Asp Phe Ile Gly Gly Ser Lys Trp Leu Trp Met Asn Gln His         
        195                 200                 205                     

tgg acc cat cac act tac acc aac cat cac gag aag gat ccc gac gcc      672
Trp Thr His His Thr Tyr Thr Asn His His Glu Lys Asp Pro Asp Ala         
    210                 215                 220                         

ctg ggt gca gag cct atg ctg ctc ttc aac gac tat ccc ttg ggt cac      720
Leu Gly Ala Glu Pro Met Leu Leu Phe Asn Asp Tyr Pro Leu Gly His         
225                 230                 235                 240         

ccc aag cga acc ctc att cat cac ttc caa gcc ttc tac tat ctg ttt      768
Pro Lys Arg Thr Leu Ile His His Phe Gln Ala Phe Tyr Tyr Leu Phe         
                245                 250                 255             

gtc ctt gct ggc tac tgg gtg tct tcg gtg ttc aac cct cag atc ctg      816
Val Leu Ala Gly Tyr Trp Val Ser Ser Val Phe Asn Pro Gln Ile Leu         
            260                 265                 270                 

gac ctc cag cac cga ggt gcc cag gct gtc ggc atg aag atg gag aac      864
Asp Leu Gln His Arg Gly Ala Gln Ala Val Gly Met Lys Met Glu Asn         
        275                 280                 285                     

gac tac att gcc aag tct cga aag tac gct atc ttc ctg cga ctc ctg      912
Asp Tyr Ile Ala Lys Ser Arg Lys Tyr Ala Ile Phe Leu Arg Leu Leu         
    290                 295                 300                         

tac atc tac acc aac att gtg gct ccc atc cag aac caa ggc ttt tcg      960
Tyr Ile Tyr Thr Asn Ile Val Ala Pro Ile Gln Asn Gln Gly Phe Ser         
305                 310                 315                 320         

ctc acc gtc gtt gct cac att ctt act atg ggt gtc gcc tcc agc ctg     1008
Leu Thr Val Val Ala His Ile Leu Thr Met Gly Val Ala Ser Ser Leu         
                325                 330                 335             

acc ctc gct act ctg ttc gcc ctc tcc cac aac ttc gag aac gca gat     1056
Thr Leu Ala Thr Leu Phe Ala Leu Ser His Asn Phe Glu Asn Ala Asp         
            340                 345                 350                 

cgg gat ccc acc tac gag gct cga aag gga ggc gag cct gtc tgt tgg     1104
Arg Asp Pro Thr Tyr Glu Ala Arg Lys Gly Gly Glu Pro Val Cys Trp         
        355                 360                 365                     

ttc aag tcg cag gtg gaa acc tcc tct act tac ggt ggc ttc att tcc     1152
Phe Lys Ser Gln Val Glu Thr Ser Ser Thr Tyr Gly Gly Phe Ile Ser         
    370                 375                 380                         

ggt tgc ctt aca ggc gga ctc aac ttt cag gtc gag cat cac ctg ttt     1200
Gly Cys Leu Thr Gly Gly Leu Asn Phe Gln Val Glu His His Leu Phe         
385                 390                 395                 400         

cct cga atg tcc tct gcc tgg tac ccc tac atc gct cct acc gtt cga     1248
Pro Arg Met Ser Ser Ala Trp Tyr Pro Tyr Ile Ala Pro Thr Val Arg         
                405                 410                 415             

gag gtc tgc aaa aag cac ggc gtc aag tac gcc tac tat ccc tgg gtg     1296
Glu Val Cys Lys Lys His Gly Val Lys Tyr Ala Tyr Tyr Pro Trp Val         
            420                 425                 430                 

tgg cag aac ctc atc tcg acc gtc aag tac ctg cat cag tcc gga act     1344
Trp Gln Asn Leu Ile Ser Thr Val Lys Tyr Leu His Gln Ser Gly Thr         
        435                 440                 445                     

ggc tcg aac tgg aag aac ggt gcc aat ccc tac tct ggc aag ctg taa     1392
Gly Ser Asn Trp Lys Asn Gly Ala Asn Pro Tyr Ser Gly Lys Leu             
    450                 455                 460                         


<210>  41
<211>  463
<212>  PRT
<213>  Peridinium sp. CCMP626

<400>  41

Met Ala Pro Asp Ala Asp Lys Leu Arg Gln Arg Lys Ala Gln Ser Ile 
1               5                   10                  15      


Gln Asp Thr Ala Asp Ser Gln Ala Thr Glu Leu Lys Ile Gly Thr Leu 
            20                  25                  30          


Lys Gly Leu Gln Gly Thr Glu Ile Val Ile Asp Gly Asp Ile Tyr Asp 
        35                  40                  45              


Ile Lys Asp Phe Asp His Pro Gly Gly Glu Ser Ile Met Thr Phe Gly 
    50                  55                  60                  


Gly Asn Asp Val Thr Ala Thr Tyr Lys Met Ile His Pro Tyr His Ser 
65                  70                  75                  80  


Lys His His Leu Glu Lys Met Lys Lys Val Gly Arg Val Pro Asp Tyr 
                85                  90                  95      


Thr Ser Glu Tyr Lys Phe Asp Thr Pro Phe Glu Arg Glu Ile Lys Gln 
            100                 105                 110         


Glu Val Phe Lys Ile Val Arg Arg Gly Arg Glu Phe Gly Thr Pro Gly 
        115                 120                 125             


Tyr Phe Phe Arg Ala Phe Cys Tyr Ile Gly Leu Phe Phe Tyr Leu Gln 
    130                 135                 140                 


Tyr Leu Trp Val Thr Thr Pro Thr Thr Phe Ala Leu Ala Ile Phe Tyr 
145                 150                 155                 160 


Gly Val Ser Gln Ala Phe Ile Gly Leu Asn Val Gln His Asp Ala Asn 
                165                 170                 175     


His Gly Ala Ala Ser Lys Lys Pro Trp Ile Asn Asn Leu Leu Gly Leu 
            180                 185                 190         


Gly Ala Asp Phe Ile Gly Gly Ser Lys Trp Leu Trp Met Asn Gln His 
        195                 200                 205             


Trp Thr His His Thr Tyr Thr Asn His His Glu Lys Asp Pro Asp Ala 
    210                 215                 220                 


Leu Gly Ala Glu Pro Met Leu Leu Phe Asn Asp Tyr Pro Leu Gly His 
225                 230                 235                 240 


Pro Lys Arg Thr Leu Ile His His Phe Gln Ala Phe Tyr Tyr Leu Phe 
                245                 250                 255     


Val Leu Ala Gly Tyr Trp Val Ser Ser Val Phe Asn Pro Gln Ile Leu 
            260                 265                 270         


Asp Leu Gln His Arg Gly Ala Gln Ala Val Gly Met Lys Met Glu Asn 
        275                 280                 285             


Asp Tyr Ile Ala Lys Ser Arg Lys Tyr Ala Ile Phe Leu Arg Leu Leu 
    290                 295                 300                 


Tyr Ile Tyr Thr Asn Ile Val Ala Pro Ile Gln Asn Gln Gly Phe Ser 
305                 310                 315                 320 


Leu Thr Val Val Ala His Ile Leu Thr Met Gly Val Ala Ser Ser Leu 
                325                 330                 335     


Thr Leu Ala Thr Leu Phe Ala Leu Ser His Asn Phe Glu Asn Ala Asp 
            340                 345                 350         


Arg Asp Pro Thr Tyr Glu Ala Arg Lys Gly Gly Glu Pro Val Cys Trp 
        355                 360                 365             


Phe Lys Ser Gln Val Glu Thr Ser Ser Thr Tyr Gly Gly Phe Ile Ser 
    370                 375                 380                 


Gly Cys Leu Thr Gly Gly Leu Asn Phe Gln Val Glu His His Leu Phe 
385                 390                 395                 400 


Pro Arg Met Ser Ser Ala Trp Tyr Pro Tyr Ile Ala Pro Thr Val Arg 
                405                 410                 415     


Glu Val Cys Lys Lys His Gly Val Lys Tyr Ala Tyr Tyr Pro Trp Val 
            420                 425                 430         


Trp Gln Asn Leu Ile Ser Thr Val Lys Tyr Leu His Gln Ser Gly Thr 
        435                 440                 445             


Gly Ser Asn Trp Lys Asn Gly Ala Asn Pro Tyr Ser Gly Lys Leu 
    450                 455                 460             


<210>  42
<211>  1362
<212>  DNA
<213>  Euglena anabaena UTEX 373


<220>
<221>  CDS
<222>  (1)..(1362)
<223>  delta-5 desaturase

<400>  42
atg gcc acc atc tct ttg act act gag caa ctt tta gaa cac cca gaa       48
Met Ala Thr Ile Ser Leu Thr Thr Glu Gln Leu Leu Glu His Pro Glu         
1               5                   10                  15              

ctg gtt gca att gat ggg gtg ttg tac gat ctc ttc gga ctg gcg aaa       96
Leu Val Ala Ile Asp Gly Val Leu Tyr Asp Leu Phe Gly Leu Ala Lys         
            20                  25                  30                  

gtg cat cca ggt ggc aac ctc att gaa gcc gcc ggt gcc tcc gac gga      144
Val His Pro Gly Gly Asn Leu Ile Glu Ala Ala Gly Ala Ser Asp Gly         
        35                  40                  45                      

acc gcc ctg ttc tac tcc atg cac cct gga gtg aag cca gag aat tcg      192
Thr Ala Leu Phe Tyr Ser Met His Pro Gly Val Lys Pro Glu Asn Ser         
    50                  55                  60                          

aag ctg ctg cag caa ttt gcc cga ggc aaa cac gaa cga agc tcg aag      240
Lys Leu Leu Gln Gln Phe Ala Arg Gly Lys His Glu Arg Ser Ser Lys         
65                  70                  75                  80          

gac cca gtg tac acc ttt gac agt ccc ttc gcc cag gat gtc aag cag      288
Asp Pro Val Tyr Thr Phe Asp Ser Pro Phe Ala Gln Asp Val Lys Gln         
                85                  90                  95              

agc gtt cgg gag gtc atg aag ggg cgc aac tgg tac gcc acg ccc ggc      336
Ser Val Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly         
            100                 105                 110                 

ttt tgg ctg cgg acc gcg ctg atc atc gcg tgc act gcc ata ggc gaa      384
Phe Trp Leu Arg Thr Ala Leu Ile Ile Ala Cys Thr Ala Ile Gly Glu         
        115                 120                 125                     

tgg tat tgg atc act acc ggg gca gtg atg tgg ggc atc ttc acc ggg      432
Trp Tyr Trp Ile Thr Thr Gly Ala Val Met Trp Gly Ile Phe Thr Gly         
    130                 135                 140                         

tac ttc cac agc cag att ggg ttg gcg att caa cac gat gcc tct cac      480
Tyr Phe His Ser Gln Ile Gly Leu Ala Ile Gln His Asp Ala Ser His         
145                 150                 155                 160         

gga gcc atc agc aaa aag ccc tgg gtg aac gcc ttt ttc gcc tac ggc      528
Gly Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Phe Phe Ala Tyr Gly         
                165                 170                 175             

atc gac gcc att gga tcc tcc cgc tgg atc tgg ctg cag tcc cac att      576
Ile Asp Ala Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile         
            180                 185                 190                 

atg cgc cac cac acc tac acc aac cag cat ggc ctg gac ctg gac gct      624
Met Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala         
        195                 200                 205                     

gcc tcg gcg gag ccg ttc att ttg ttc cac tcc tac ccg gca aca aat      672
Ala Ser Ala Glu Pro Phe Ile Leu Phe His Ser Tyr Pro Ala Thr Asn         
    210                 215                 220                         

gcg tca cga aag tgg tac cat cgg ttc cag gcg tgg tac atg tac atc      720
Ala Ser Arg Lys Trp Tyr His Arg Phe Gln Ala Trp Tyr Met Tyr Ile         
225                 230                 235                 240         

gtt ttg ggg atg tat ggt gtg tcg atg gtg tac aat ccg atg tac ttg      768
Val Leu Gly Met Tyr Gly Val Ser Met Val Tyr Asn Pro Met Tyr Leu         
                245                 250                 255             

ttc acg atg cag cac aac gac aca atc cca gag gcc acc tct ctt aga      816
Phe Thr Met Gln His Asn Asp Thr Ile Pro Glu Ala Thr Ser Leu Arg         
            260                 265                 270                 

cca ggc agc ttt ttc aac cgg cag cgc gcc ttc gcc gtt tcc ctc cgc      864
Pro Gly Ser Phe Phe Asn Arg Gln Arg Ala Phe Ala Val Ser Leu Arg         
        275                 280                 285                     

cta ctg ttc atc ttc cgc aac gcc ttc ctc ccc tgg tac atc gcg ggc      912
Leu Leu Phe Ile Phe Arg Asn Ala Phe Leu Pro Trp Tyr Ile Ala Gly         
    290                 295                 300                         

gcc tct ccg ctg ctc acc atc ctg ctg gtg cca acg gtc aca ggc atc      960
Ala Ser Pro Leu Leu Thr Ile Leu Leu Val Pro Thr Val Thr Gly Ile         
305                 310                 315                 320         

ttc ttg aca ttt gtt ttt gtg ctg tcc cat aac ttt gaa ggc gct gag     1008
Phe Leu Thr Phe Val Phe Val Leu Ser His Asn Phe Glu Gly Ala Glu         
                325                 330                 335             

cgg acc ccc gaa aag aac tgc aag gcc aaa agg gcc aag gag ggg aag     1056
Arg Thr Pro Glu Lys Asn Cys Lys Ala Lys Arg Ala Lys Glu Gly Lys         
            340                 345                 350                 

gag gtc cgc gat gta gag gag gac cgg gtg gac tgg tac cgg gcg cag     1104
Glu Val Arg Asp Val Glu Glu Asp Arg Val Asp Trp Tyr Arg Ala Gln         
        355                 360                 365                     

gcc gag acc gcg gcg acc tac ggg ggc agc gtc ggg atg atg ctg acc     1152
Ala Glu Thr Ala Ala Thr Tyr Gly Gly Ser Val Gly Met Met Leu Thr         
    370                 375                 380                         

ggc ggt ttg aac ctg cag atc gag cac cac ttg ttc ccc cgc atg tcc     1200
Gly Gly Leu Asn Leu Gln Ile Glu His His Leu Phe Pro Arg Met Ser         
385                 390                 395                 400         

tct tgg cac tac ccc ttc atc caa gat acg gtg cgg gaa tgt tgc aag     1248
Ser Trp His Tyr Pro Phe Ile Gln Asp Thr Val Arg Glu Cys Cys Lys         
                405                 410                 415             

cgc cat ggc gtg cgc tac aca tac tac ccg acc atc ctg gag aat ata     1296
Arg His Gly Val Arg Tyr Thr Tyr Tyr Pro Thr Ile Leu Glu Asn Ile         
            420                 425                 430                 

atg tcc acg ctc cgc tac atg cag aag gtg ggc gtg gcc cac aca att     1344
Met Ser Thr Leu Arg Tyr Met Gln Lys Val Gly Val Ala His Thr Ile         
        435                 440                 445                     

cag gat gcc cag gaa ttc                                             1362
Gln Asp Ala Gln Glu Phe                                                 
    450                                                                 


<210>  43
<211>  454
<212>  PRT
<213>  Euglena anabaena UTEX 373

<400>  43

Met Ala Thr Ile Ser Leu Thr Thr Glu Gln Leu Leu Glu His Pro Glu 
1               5                   10                  15      


Leu Val Ala Ile Asp Gly Val Leu Tyr Asp Leu Phe Gly Leu Ala Lys 
            20                  25                  30          


Val His Pro Gly Gly Asn Leu Ile Glu Ala Ala Gly Ala Ser Asp Gly 
        35                  40                  45              


Thr Ala Leu Phe Tyr Ser Met His Pro Gly Val Lys Pro Glu Asn Ser 
    50                  55                  60                  


Lys Leu Leu Gln Gln Phe Ala Arg Gly Lys His Glu Arg Ser Ser Lys 
65                  70                  75                  80  


Asp Pro Val Tyr Thr Phe Asp Ser Pro Phe Ala Gln Asp Val Lys Gln 
                85                  90                  95      


Ser Val Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly 
            100                 105                 110         


Phe Trp Leu Arg Thr Ala Leu Ile Ile Ala Cys Thr Ala Ile Gly Glu 
        115                 120                 125             


Trp Tyr Trp Ile Thr Thr Gly Ala Val Met Trp Gly Ile Phe Thr Gly 
    130                 135                 140                 


Tyr Phe His Ser Gln Ile Gly Leu Ala Ile Gln His Asp Ala Ser His 
145                 150                 155                 160 


Gly Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Phe Phe Ala Tyr Gly 
                165                 170                 175     


Ile Asp Ala Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile 
            180                 185                 190         


Met Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala 
        195                 200                 205             


Ala Ser Ala Glu Pro Phe Ile Leu Phe His Ser Tyr Pro Ala Thr Asn 
    210                 215                 220                 


Ala Ser Arg Lys Trp Tyr His Arg Phe Gln Ala Trp Tyr Met Tyr Ile 
225                 230                 235                 240 


Val Leu Gly Met Tyr Gly Val Ser Met Val Tyr Asn Pro Met Tyr Leu 
                245                 250                 255     


Phe Thr Met Gln His Asn Asp Thr Ile Pro Glu Ala Thr Ser Leu Arg 
            260                 265                 270         


Pro Gly Ser Phe Phe Asn Arg Gln Arg Ala Phe Ala Val Ser Leu Arg 
        275                 280                 285             


Leu Leu Phe Ile Phe Arg Asn Ala Phe Leu Pro Trp Tyr Ile Ala Gly 
    290                 295                 300                 


Ala Ser Pro Leu Leu Thr Ile Leu Leu Val Pro Thr Val Thr Gly Ile 
305                 310                 315                 320 


Phe Leu Thr Phe Val Phe Val Leu Ser His Asn Phe Glu Gly Ala Glu 
                325                 330                 335     


Arg Thr Pro Glu Lys Asn Cys Lys Ala Lys Arg Ala Lys Glu Gly Lys 
            340                 345                 350         


Glu Val Arg Asp Val Glu Glu Asp Arg Val Asp Trp Tyr Arg Ala Gln 
        355                 360                 365             


Ala Glu Thr Ala Ala Thr Tyr Gly Gly Ser Val Gly Met Met Leu Thr 
    370                 375                 380                 


Gly Gly Leu Asn Leu Gln Ile Glu His His Leu Phe Pro Arg Met Ser 
385                 390                 395                 400 


Ser Trp His Tyr Pro Phe Ile Gln Asp Thr Val Arg Glu Cys Cys Lys 
                405                 410                 415     


Arg His Gly Val Arg Tyr Thr Tyr Tyr Pro Thr Ile Leu Glu Asn Ile 
            420                 425                 430         


Met Ser Thr Leu Arg Tyr Met Gln Lys Val Gly Val Ala His Thr Ile 
        435                 440                 445             


Gln Asp Ala Gln Glu Phe 
    450                 


<210>  44
<211>  1362
<212>  DNA
<213>  Euglena anabaena UTEX 373


<220>
<221>  CDS
<222>  (1)..(1362)
<223>  synthetic delta-5 desaturase (codon-optimized for Yarrowia 
       lipolytica)

<400>  44
atg gcc acc atc tcc ctg act acc gag cag ctc ctg gaa cac ccc gag       48
Met Ala Thr Ile Ser Leu Thr Thr Glu Gln Leu Leu Glu His Pro Glu         
1               5                   10                  15              

ctc gtt gcc atc gac gga gtc ctg tac gat ctc ttc ggt ctg gcc aag       96
Leu Val Ala Ile Asp Gly Val Leu Tyr Asp Leu Phe Gly Leu Ala Lys         
            20                  25                  30                  

gtg cat cca gga ggc aac ctc atc gaa gct gcc ggt gca tcc gac gga      144
Val His Pro Gly Gly Asn Leu Ile Glu Ala Ala Gly Ala Ser Asp Gly         
        35                  40                  45                      

acc gct ctg ttc tac tcc atg cat cct gga gtc aag cca gag aac tcg      192
Thr Ala Leu Phe Tyr Ser Met His Pro Gly Val Lys Pro Glu Asn Ser         
    50                  55                  60                          

aag ctt ctg cag caa ttt gcc cga ggc aag cac gaa cga agc tcc aag      240
Lys Leu Leu Gln Gln Phe Ala Arg Gly Lys His Glu Arg Ser Ser Lys         
65                  70                  75                  80          

gat ccc gtg tac acc ttc gac tct ccc ttt gct cag gac gtc aag cag      288
Asp Pro Val Tyr Thr Phe Asp Ser Pro Phe Ala Gln Asp Val Lys Gln         
                85                  90                  95              

tcc gtt cga gag gtc atg aag ggt cga aac tgg tac gcc act cct ggc      336
Ser Val Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly         
            100                 105                 110                 

ttc tgg ctg aga acc gca ctc atc atc gct tgt act gcc att ggc gag      384
Phe Trp Leu Arg Thr Ala Leu Ile Ile Ala Cys Thr Ala Ile Gly Glu         
        115                 120                 125                     

tgg tac tgg atc aca acc gga gca gtg atg tgg ggt atc ttt act gga      432
Trp Tyr Trp Ile Thr Thr Gly Ala Val Met Trp Gly Ile Phe Thr Gly         
    130                 135                 140                         

tac ttc cac tcg cag att ggc ttg gcc att caa cac gat gct tct cac      480
Tyr Phe His Ser Gln Ile Gly Leu Ala Ile Gln His Asp Ala Ser His         
145                 150                 155                 160         

gga gcc atc agc aaa aag ccc tgg gtc aac gcc ttt ttc gct tat ggc      528
Gly Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Phe Phe Ala Tyr Gly         
                165                 170                 175             

atc gac gcc att ggt tcc tct cgt tgg atc tgg ctg cag tcc cac att      576
Ile Asp Ala Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile         
            180                 185                 190                 

atg cga cat cac act tac acc aac cag cat ggc ctc gac ctg gat gct      624
Met Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala         
        195                 200                 205                     

gcc tcg gca gag ccg ttc atc ttg ttc cac tcc tat cct gct acc aac      672
Ala Ser Ala Glu Pro Phe Ile Leu Phe His Ser Tyr Pro Ala Thr Asn         
    210                 215                 220                         

gcc tct cga aag tgg tac cac cga ttt cag gcg tgg tac atg tac atc      720
Ala Ser Arg Lys Trp Tyr His Arg Phe Gln Ala Trp Tyr Met Tyr Ile         
225                 230                 235                 240         

gtt ctg gga atg tat ggt gtc tcg atg gtg tac aat ccc atg tac ctc      768
Val Leu Gly Met Tyr Gly Val Ser Met Val Tyr Asn Pro Met Tyr Leu         
                245                 250                 255             

ttc aca atg cag cac aac gac acc att ccc gag gcc act tct ctc aga      816
Phe Thr Met Gln His Asn Asp Thr Ile Pro Glu Ala Thr Ser Leu Arg         
            260                 265                 270                 

cca ggc agc ttt ttc aat cgg cag cga gct ttc gcc gtt tcc ctt cga      864
Pro Gly Ser Phe Phe Asn Arg Gln Arg Ala Phe Ala Val Ser Leu Arg         
        275                 280                 285                     

ctg ctc ttc atc ttc cga aac gcc ttt ctt ccc tgg tac att gct ggt      912
Leu Leu Phe Ile Phe Arg Asn Ala Phe Leu Pro Trp Tyr Ile Ala Gly         
    290                 295                 300                         

gcc tct cct ctg ctc acc att ctt ctg gtg ccc acg gtc aca ggc atc      960
Ala Ser Pro Leu Leu Thr Ile Leu Leu Val Pro Thr Val Thr Gly Ile         
305                 310                 315                 320         

ttc ctc acc ttt gtg ttc gtt ctg tcc cat aac ttc gag gga gcc gaa     1008
Phe Leu Thr Phe Val Phe Val Leu Ser His Asn Phe Glu Gly Ala Glu         
                325                 330                 335             

cgg acc cca gag aag aac tgc aag gcc aaa cga gct aag gaa ggc aag     1056
Arg Thr Pro Glu Lys Asn Cys Lys Ala Lys Arg Ala Lys Glu Gly Lys         
            340                 345                 350                 

gag gtc aga gac gtg gaa gag gat cga gtc gac tgg tac cga gca cag     1104
Glu Val Arg Asp Val Glu Glu Asp Arg Val Asp Trp Tyr Arg Ala Gln         
        355                 360                 365                     

gcc gag act gct gcc acc tac ggt ggc agc gtg gga atg atg ctt aca     1152
Ala Glu Thr Ala Ala Thr Tyr Gly Gly Ser Val Gly Met Met Leu Thr         
    370                 375                 380                         

ggc ggt ctc aac ctg cag atc gag cat cac ttg ttt ccc cga atg tcc     1200
Gly Gly Leu Asn Leu Gln Ile Glu His His Leu Phe Pro Arg Met Ser         
385                 390                 395                 400         

tct tgg cac tat ccc ttc att caa gac acc gtt cgg gag tgt tgc aag     1248
Ser Trp His Tyr Pro Phe Ile Gln Asp Thr Val Arg Glu Cys Cys Lys         
                405                 410                 415             

cga cat ggc gtc cgt tac aca tac tat cct acc att ctc gag aac atc     1296
Arg His Gly Val Arg Tyr Thr Tyr Tyr Pro Thr Ile Leu Glu Asn Ile         
            420                 425                 430                 

atg tcc act ctt cga tac atg cag aag gtg ggt gtt gct cac acc att     1344
Met Ser Thr Leu Arg Tyr Met Gln Lys Val Gly Val Ala His Thr Ile         
        435                 440                 445                     

cag gat gcc cag gag ttc                                             1362
Gln Asp Ala Gln Glu Phe                                                 
    450                                                                 


<210>  45
<211>  454
<212>  PRT
<213>  Euglena anabaena UTEX 373

<400>  45

Met Ala Thr Ile Ser Leu Thr Thr Glu Gln Leu Leu Glu His Pro Glu 
1               5                   10                  15      


Leu Val Ala Ile Asp Gly Val Leu Tyr Asp Leu Phe Gly Leu Ala Lys 
            20                  25                  30          


Val His Pro Gly Gly Asn Leu Ile Glu Ala Ala Gly Ala Ser Asp Gly 
        35                  40                  45              


Thr Ala Leu Phe Tyr Ser Met His Pro Gly Val Lys Pro Glu Asn Ser 
    50                  55                  60                  


Lys Leu Leu Gln Gln Phe Ala Arg Gly Lys His Glu Arg Ser Ser Lys 
65                  70                  75                  80  


Asp Pro Val Tyr Thr Phe Asp Ser Pro Phe Ala Gln Asp Val Lys Gln 
                85                  90                  95      


Ser Val Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly 
            100                 105                 110         


Phe Trp Leu Arg Thr Ala Leu Ile Ile Ala Cys Thr Ala Ile Gly Glu 
        115                 120                 125             


Trp Tyr Trp Ile Thr Thr Gly Ala Val Met Trp Gly Ile Phe Thr Gly 
    130                 135                 140                 


Tyr Phe His Ser Gln Ile Gly Leu Ala Ile Gln His Asp Ala Ser His 
145                 150                 155                 160 


Gly Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Phe Phe Ala Tyr Gly 
                165                 170                 175     


Ile Asp Ala Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile 
            180                 185                 190         


Met Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala 
        195                 200                 205             


Ala Ser Ala Glu Pro Phe Ile Leu Phe His Ser Tyr Pro Ala Thr Asn 
    210                 215                 220                 


Ala Ser Arg Lys Trp Tyr His Arg Phe Gln Ala Trp Tyr Met Tyr Ile 
225                 230                 235                 240 


Val Leu Gly Met Tyr Gly Val Ser Met Val Tyr Asn Pro Met Tyr Leu 
                245                 250                 255     


Phe Thr Met Gln His Asn Asp Thr Ile Pro Glu Ala Thr Ser Leu Arg 
            260                 265                 270         


Pro Gly Ser Phe Phe Asn Arg Gln Arg Ala Phe Ala Val Ser Leu Arg 
        275                 280                 285             


Leu Leu Phe Ile Phe Arg Asn Ala Phe Leu Pro Trp Tyr Ile Ala Gly 
    290                 295                 300                 


Ala Ser Pro Leu Leu Thr Ile Leu Leu Val Pro Thr Val Thr Gly Ile 
305                 310                 315                 320 


Phe Leu Thr Phe Val Phe Val Leu Ser His Asn Phe Glu Gly Ala Glu 
                325                 330                 335     


Arg Thr Pro Glu Lys Asn Cys Lys Ala Lys Arg Ala Lys Glu Gly Lys 
            340                 345                 350         


Glu Val Arg Asp Val Glu Glu Asp Arg Val Asp Trp Tyr Arg Ala Gln 
        355                 360                 365             


Ala Glu Thr Ala Ala Thr Tyr Gly Gly Ser Val Gly Met Met Leu Thr 
    370                 375                 380                 


Gly Gly Leu Asn Leu Gln Ile Glu His His Leu Phe Pro Arg Met Ser 
385                 390                 395                 400 


Ser Trp His Tyr Pro Phe Ile Gln Asp Thr Val Arg Glu Cys Cys Lys 
                405                 410                 415     


Arg His Gly Val Arg Tyr Thr Tyr Tyr Pro Thr Ile Leu Glu Asn Ile 
            420                 425                 430         


Met Ser Thr Leu Arg Tyr Met Gln Lys Val Gly Val Ala His Thr Ile 
        435                 440                 445             


Gln Asp Ala Gln Glu Phe 
    450                 


<210>  46
<211>  1086
<212>  DNA
<213>  Phytophthora ramorum


<220>
<221>  CDS
<222>  (1)..(1086)
<223>  delta-17 desaturase

<300>
<302>  DELTA-17 DESATURASES AND THEIR USE IN MAKING POLYUNSATURATED 
       FATTY ACIDS
<310>  WO 2007/123999
<311>  2007-04-19
<312>  2007-11-01
<313>  (1)..(1086)

<300>
<302>  DELTA-17 DESATURASES AND THEIR USE IN MAKING POLYUNSATURATED 
       FATTY ACIDS
<310>  US 2007-0249026-A1
<311>  2007-04-18
<312>  2007-10-25
<313>  (1)..(1086)

<400>  46
atg gcg act aag cag ccg tac cag ttc ccg acc ctg acg gag atc aag       48
Met Ala Thr Lys Gln Pro Tyr Gln Phe Pro Thr Leu Thr Glu Ile Lys         
1               5                   10                  15              

cgg tcg ctg ccc agc gag tgc ttt gag gcc tcg gtg ccg ctg tcg ctc       96
Arg Ser Leu Pro Ser Glu Cys Phe Glu Ala Ser Val Pro Leu Ser Leu         
            20                  25                  30                  

tac tac acg gtg cgc atc gtg gcc atc gcc gtg gcg ctg gcg ttc ggc      144
Tyr Tyr Thr Val Arg Ile Val Ala Ile Ala Val Ala Leu Ala Phe Gly         
        35                  40                  45                      

ctc aac tac gcg cgc gcg ctg ccc gtg gtc gag agc ttg tgg gcg ctg      192
Leu Asn Tyr Ala Arg Ala Leu Pro Val Val Glu Ser Leu Trp Ala Leu         
    50                  55                  60                          

gac gct gcg ctc tgc tgc ggt tac gtg ctg ctg cag ggc atc gtg ttc      240
Asp Ala Ala Leu Cys Cys Gly Tyr Val Leu Leu Gln Gly Ile Val Phe         
65                  70                  75                  80          

tgg ggc ttc ttc acg gtg ggc cat gac gcc ggc cac ggc gcc ttc tcg      288
Trp Gly Phe Phe Thr Val Gly His Asp Ala Gly His Gly Ala Phe Ser         
                85                  90                  95              

cgt tac cac ctg ctc aac ttc gtg gtg ggc acc ttc atc cac tcg ctc      336
Arg Tyr His Leu Leu Asn Phe Val Val Gly Thr Phe Ile His Ser Leu         
            100                 105                 110                 

atc ctc acg ccc ttc gag tcg tgg aag ctc acg cac cgc cac cac cac      384
Ile Leu Thr Pro Phe Glu Ser Trp Lys Leu Thr His Arg His His His         
        115                 120                 125                     

aag aac acg ggc aac att gac cgc gac gag atc ttc tac ccg cag cgc      432
Lys Asn Thr Gly Asn Ile Asp Arg Asp Glu Ile Phe Tyr Pro Gln Arg         
    130                 135                 140                         

aag gcc gac gac cac ccg ctg tcg cgc aac ctc gtg ctg gcg ctc ggc      480
Lys Ala Asp Asp His Pro Leu Ser Arg Asn Leu Val Leu Ala Leu Gly         
145                 150                 155                 160         

gcc gcg tgg ttc gcc tac ctg gtc gag ggc ttc ccg ccc cgc aag gtc      528
Ala Ala Trp Phe Ala Tyr Leu Val Glu Gly Phe Pro Pro Arg Lys Val         
                165                 170                 175             

aac cac ttc aac cca ttc gag ccg ctg ttt gtg cgc cag gtg gcc gcc      576
Asn His Phe Asn Pro Phe Glu Pro Leu Phe Val Arg Gln Val Ala Ala         
            180                 185                 190                 

gtc gtc atc tcg ctc tcc gcg cac ttc gcc gtg ttg gcg ctg tcc gtg      624
Val Val Ile Ser Leu Ser Ala His Phe Ala Val Leu Ala Leu Ser Val         
        195                 200                 205                     

tat ctg agc ttc cag ttc ggt ctc aag acc atg gcg ctc tac tac tac      672
Tyr Leu Ser Phe Gln Phe Gly Leu Lys Thr Met Ala Leu Tyr Tyr Tyr         
    210                 215                 220                         

ggc ccc gtc ttc gtg ttc ggc agc atg ctt gtg atc acc acc ttc ctg      720
Gly Pro Val Phe Val Phe Gly Ser Met Leu Val Ile Thr Thr Phe Leu         
225                 230                 235                 240         

cat cac aat gac gag gag acc cca tgg tac gga gac tcc gac tgg acc      768
His His Asn Asp Glu Glu Thr Pro Trp Tyr Gly Asp Ser Asp Trp Thr         
                245                 250                 255             

tac gtc aag ggc aac ctg tcg tcc gtg gac cgg tcc tac ggc gcg ttc      816
Tyr Val Lys Gly Asn Leu Ser Ser Val Asp Arg Ser Tyr Gly Ala Phe         
            260                 265                 270                 

atc gac aac ctg agc cac aac atc ggc acg cac cag atc cac cac ctc      864
Ile Asp Asn Leu Ser His Asn Ile Gly Thr His Gln Ile His His Leu         
        275                 280                 285                     

ttc ccc atc atc ccg cac tac aag ctc aac cgc gct acg gcg gca ttc      912
Phe Pro Ile Ile Pro His Tyr Lys Leu Asn Arg Ala Thr Ala Ala Phe         
    290                 295                 300                         

cac cag gcc ttc ccc gag ctc gtg cgc aag agc gac gag ccg atc ctc      960
His Gln Ala Phe Pro Glu Leu Val Arg Lys Ser Asp Glu Pro Ile Leu         
305                 310                 315                 320         

aag gcc ttc tgg cgc gtc ggc cga ctg tac gcc aac tac ggc gtc gtg     1008
Lys Ala Phe Trp Arg Val Gly Arg Leu Tyr Ala Asn Tyr Gly Val Val         
                325                 330                 335             

gac ccg gac gcc aag ctc ttc acg ctc aag gag gcc aag gcg gcg tcc     1056
Asp Pro Asp Ala Lys Leu Phe Thr Leu Lys Glu Ala Lys Ala Ala Ser         
            340                 345                 350                 

gag gcg gcg acc aag acc aag gcc acc taa                             1086
Glu Ala Ala Thr Lys Thr Lys Ala Thr                                     
        355                 360                                         


<210>  47
<211>  361
<212>  PRT
<213>  Phytophthora ramorum

<400>  47

Met Ala Thr Lys Gln Pro Tyr Gln Phe Pro Thr Leu Thr Glu Ile Lys 
1               5                   10                  15      


Arg Ser Leu Pro Ser Glu Cys Phe Glu Ala Ser Val Pro Leu Ser Leu 
            20                  25                  30          


Tyr Tyr Thr Val Arg Ile Val Ala Ile Ala Val Ala Leu Ala Phe Gly 
        35                  40                  45              


Leu Asn Tyr Ala Arg Ala Leu Pro Val Val Glu Ser Leu Trp Ala Leu 
    50                  55                  60                  


Asp Ala Ala Leu Cys Cys Gly Tyr Val Leu Leu Gln Gly Ile Val Phe 
65                  70                  75                  80  


Trp Gly Phe Phe Thr Val Gly His Asp Ala Gly His Gly Ala Phe Ser 
                85                  90                  95      


Arg Tyr His Leu Leu Asn Phe Val Val Gly Thr Phe Ile His Ser Leu 
            100                 105                 110         


Ile Leu Thr Pro Phe Glu Ser Trp Lys Leu Thr His Arg His His His 
        115                 120                 125             


Lys Asn Thr Gly Asn Ile Asp Arg Asp Glu Ile Phe Tyr Pro Gln Arg 
    130                 135                 140                 


Lys Ala Asp Asp His Pro Leu Ser Arg Asn Leu Val Leu Ala Leu Gly 
145                 150                 155                 160 


Ala Ala Trp Phe Ala Tyr Leu Val Glu Gly Phe Pro Pro Arg Lys Val 
                165                 170                 175     


Asn His Phe Asn Pro Phe Glu Pro Leu Phe Val Arg Gln Val Ala Ala 
            180                 185                 190         


Val Val Ile Ser Leu Ser Ala His Phe Ala Val Leu Ala Leu Ser Val 
        195                 200                 205             


Tyr Leu Ser Phe Gln Phe Gly Leu Lys Thr Met Ala Leu Tyr Tyr Tyr 
    210                 215                 220                 


Gly Pro Val Phe Val Phe Gly Ser Met Leu Val Ile Thr Thr Phe Leu 
225                 230                 235                 240 


His His Asn Asp Glu Glu Thr Pro Trp Tyr Gly Asp Ser Asp Trp Thr 
                245                 250                 255     


Tyr Val Lys Gly Asn Leu Ser Ser Val Asp Arg Ser Tyr Gly Ala Phe 
            260                 265                 270         


Ile Asp Asn Leu Ser His Asn Ile Gly Thr His Gln Ile His His Leu 
        275                 280                 285             


Phe Pro Ile Ile Pro His Tyr Lys Leu Asn Arg Ala Thr Ala Ala Phe 
    290                 295                 300                 


His Gln Ala Phe Pro Glu Leu Val Arg Lys Ser Asp Glu Pro Ile Leu 
305                 310                 315                 320 


Lys Ala Phe Trp Arg Val Gly Arg Leu Tyr Ala Asn Tyr Gly Val Val 
                325                 330                 335     


Asp Pro Asp Ala Lys Leu Phe Thr Leu Lys Glu Ala Lys Ala Ala Ser 
            340                 345                 350         


Glu Ala Ala Thr Lys Thr Lys Ala Thr 
        355                 360     


<210>  48
<211>  1086
<212>  DNA
<213>  Phytophthora ramorum


<220>
<221>  CDS
<222>  (1)..(1086)
<223>  synthetic delta-17 desaturase (codon-optimized for Yarrowia 
       lipolytica)

<300>
<302>  DELTA-17 DESATURASES AND THEIR USE IN MAKING POLYUNSATURATED 
       FATTY ACIDS
<310>  WO 2007/123999
<311>  2007-04-19
<312>  2007-11-01
<313>  (1)..(1086)

<300>
<302>  DELTA-17 DESATURASES AND THEIR USE IN MAKING POLYUNSATURATED 
       FATTY ACIDS
<310>  US 2007-0249026-A1
<311>  2007-04-18
<312>  2007-10-25
<313>  (1)..(1086)

<400>  48
atg gct acc aag cag ccc tac cag ttc cct act ctg acc gag atc aag       48
Met Ala Thr Lys Gln Pro Tyr Gln Phe Pro Thr Leu Thr Glu Ile Lys         
1               5                   10                  15              

cga tct ctt ccc tcc gag tgc ttt gaa gcc tcg gtc cct ctg tcc ttg       96
Arg Ser Leu Pro Ser Glu Cys Phe Glu Ala Ser Val Pro Leu Ser Leu         
            20                  25                  30                  

tac tac acc gtg cga atc gtc gct att gcc gtt gct ctg gcc ttc gga      144
Tyr Tyr Thr Val Arg Ile Val Ala Ile Ala Val Ala Leu Ala Phe Gly         
        35                  40                  45                      

ctc aac tac gct cga gcc ctt ccc gtg gtc gag tct ctg tgg gca ctc      192
Leu Asn Tyr Ala Arg Ala Leu Pro Val Val Glu Ser Leu Trp Ala Leu         
    50                  55                  60                          

gac gct gcc ctt tgt tgc ggt tac gtt ctg ctc caa ggc att gtc ttc      240
Asp Ala Ala Leu Cys Cys Gly Tyr Val Leu Leu Gln Gly Ile Val Phe         
65                  70                  75                  80          

tgg gga ttc ttt acc gtg ggt cac gat gct gga cat ggt gcc ttc tct      288
Trp Gly Phe Phe Thr Val Gly His Asp Ala Gly His Gly Ala Phe Ser         
                85                  90                  95              

cga tac cac ctg ctc aac ttt gtc gtt ggc acc ttt atc cac tcc ctc      336
Arg Tyr His Leu Leu Asn Phe Val Val Gly Thr Phe Ile His Ser Leu         
            100                 105                 110                 

att ctt act ccc ttc gag tcg tgg aag ctc aca cat cga cac cat cac      384
Ile Leu Thr Pro Phe Glu Ser Trp Lys Leu Thr His Arg His His His         
        115                 120                 125                     

aag aac acc gga aac atc gac cga gac gaa atc ttc tac cct cag cga      432
Lys Asn Thr Gly Asn Ile Asp Arg Asp Glu Ile Phe Tyr Pro Gln Arg         
    130                 135                 140                         

aag gcc gac gat cat cct ctg tct cga aac ctc gtc ctg gct ctc ggt      480
Lys Ala Asp Asp His Pro Leu Ser Arg Asn Leu Val Leu Ala Leu Gly         
145                 150                 155                 160         

gcc gct tgg ttt gcc tac ctt gtc gag ggc ttt cct ccc cga aag gtc      528
Ala Ala Trp Phe Ala Tyr Leu Val Glu Gly Phe Pro Pro Arg Lys Val         
                165                 170                 175             

aac cac ttc aac ccc ttc gaa cct ctg ttt gtg cga cag gtg gct gcc      576
Asn His Phe Asn Pro Phe Glu Pro Leu Phe Val Arg Gln Val Ala Ala         
            180                 185                 190                 

gtt gtc att tcc ctc tct gct cac ttc gcc gtc ctg gca ctg tcc gtg      624
Val Val Ile Ser Leu Ser Ala His Phe Ala Val Leu Ala Leu Ser Val         
        195                 200                 205                     

tat ctg agc ttt cag ttc ggt ctc aag aca atg gct ctg tac tac tat      672
Tyr Leu Ser Phe Gln Phe Gly Leu Lys Thr Met Ala Leu Tyr Tyr Tyr         
    210                 215                 220                         

gga ccc gtc ttc gtg ttc ggc tcc atg ctc gtc att act acc ttt ctg      720
Gly Pro Val Phe Val Phe Gly Ser Met Leu Val Ile Thr Thr Phe Leu         
225                 230                 235                 240         

cat cac aat gac gag gaa act cct tgg tac gga gat tcc gac tgg acc      768
His His Asn Asp Glu Glu Thr Pro Trp Tyr Gly Asp Ser Asp Trp Thr         
                245                 250                 255             

tac gtc aag ggc aac ttg tct tcc gtg gac cga tct tac ggt gcc ttc      816
Tyr Val Lys Gly Asn Leu Ser Ser Val Asp Arg Ser Tyr Gly Ala Phe         
            260                 265                 270                 

atc gac aac ctc tcg cac aac att ggc aca cac cag atc cac cat ctg      864
Ile Asp Asn Leu Ser His Asn Ile Gly Thr His Gln Ile His His Leu         
        275                 280                 285                     

ttt ccc atc att cct cac tac aag ctc aac cga gcc acc gct gcc ttc      912
Phe Pro Ile Ile Pro His Tyr Lys Leu Asn Arg Ala Thr Ala Ala Phe         
    290                 295                 300                         

cac cag gcc ttt ccc gaa ctt gtc cga aag agc gac gag ccc att ctc      960
His Gln Ala Phe Pro Glu Leu Val Arg Lys Ser Asp Glu Pro Ile Leu         
305                 310                 315                 320         

aag gct ttc tgg aga gtt ggt cga ctt tac gcc aac tac gga gtc gtg     1008
Lys Ala Phe Trp Arg Val Gly Arg Leu Tyr Ala Asn Tyr Gly Val Val         
                325                 330                 335             

gat ccc gac gca aag ctg ttt act ctc aag gag gcc aaa gct gcc tcc     1056
Asp Pro Asp Ala Lys Leu Phe Thr Leu Lys Glu Ala Lys Ala Ala Ser         
            340                 345                 350                 

gag gct gcc acc aag acc aag gct act taa                             1086
Glu Ala Ala Thr Lys Thr Lys Ala Thr                                     
        355                 360                                         


<210>  49
<211>  361
<212>  PRT
<213>  Phytophthora ramorum

<400>  49

Met Ala Thr Lys Gln Pro Tyr Gln Phe Pro Thr Leu Thr Glu Ile Lys 
1               5                   10                  15      


Arg Ser Leu Pro Ser Glu Cys Phe Glu Ala Ser Val Pro Leu Ser Leu 
            20                  25                  30          


Tyr Tyr Thr Val Arg Ile Val Ala Ile Ala Val Ala Leu Ala Phe Gly 
        35                  40                  45              


Leu Asn Tyr Ala Arg Ala Leu Pro Val Val Glu Ser Leu Trp Ala Leu 
    50                  55                  60                  


Asp Ala Ala Leu Cys Cys Gly Tyr Val Leu Leu Gln Gly Ile Val Phe 
65                  70                  75                  80  


Trp Gly Phe Phe Thr Val Gly His Asp Ala Gly His Gly Ala Phe Ser 
                85                  90                  95      


Arg Tyr His Leu Leu Asn Phe Val Val Gly Thr Phe Ile His Ser Leu 
            100                 105                 110         


Ile Leu Thr Pro Phe Glu Ser Trp Lys Leu Thr His Arg His His His 
        115                 120                 125             


Lys Asn Thr Gly Asn Ile Asp Arg Asp Glu Ile Phe Tyr Pro Gln Arg 
    130                 135                 140                 


Lys Ala Asp Asp His Pro Leu Ser Arg Asn Leu Val Leu Ala Leu Gly 
145                 150                 155                 160 


Ala Ala Trp Phe Ala Tyr Leu Val Glu Gly Phe Pro Pro Arg Lys Val 
                165                 170                 175     


Asn His Phe Asn Pro Phe Glu Pro Leu Phe Val Arg Gln Val Ala Ala 
            180                 185                 190         


Val Val Ile Ser Leu Ser Ala His Phe Ala Val Leu Ala Leu Ser Val 
        195                 200                 205             


Tyr Leu Ser Phe Gln Phe Gly Leu Lys Thr Met Ala Leu Tyr Tyr Tyr 
    210                 215                 220                 


Gly Pro Val Phe Val Phe Gly Ser Met Leu Val Ile Thr Thr Phe Leu 
225                 230                 235                 240 


His His Asn Asp Glu Glu Thr Pro Trp Tyr Gly Asp Ser Asp Trp Thr 
                245                 250                 255     


Tyr Val Lys Gly Asn Leu Ser Ser Val Asp Arg Ser Tyr Gly Ala Phe 
            260                 265                 270         


Ile Asp Asn Leu Ser His Asn Ile Gly Thr His Gln Ile His His Leu 
        275                 280                 285             


Phe Pro Ile Ile Pro His Tyr Lys Leu Asn Arg Ala Thr Ala Ala Phe 
    290                 295                 300                 


His Gln Ala Phe Pro Glu Leu Val Arg Lys Ser Asp Glu Pro Ile Leu 
305                 310                 315                 320 


Lys Ala Phe Trp Arg Val Gly Arg Leu Tyr Ala Asn Tyr Gly Val Val 
                325                 330                 335     


Asp Pro Asp Ala Lys Leu Phe Thr Leu Lys Glu Ala Lys Ala Ala Ser 
            340                 345                 350         


Glu Ala Ala Thr Lys Thr Lys Ala Thr 
        355                 360     


<210>  50
<211>  1080
<212>  DNA
<213>  Pythium aphanidermatum  


<220>
<221>  CDS
<222>  (1)..(1080)
<223>  delta-17 desaturase

<300>
<302>  DELTA-17 DESATURASES AND THEIR USE IN MAKING POLYUNSATURATED 
       FATTY ACIDS
<310>  WO 2008/054565
<311>  2007-07-19
<312>  2008-05-08
<313>  (1)..(1080)

<300>
<302>  DELTA-17 DESATURASES AND THEIR USE IN MAKING POLYUNSATURATED 
       FATTY ACIDS
<310>  US 2008-0125326-A1
<311>  2007-07-19
<312>  2008-05-29
<313>  (1)..(1080)

<400>  50
atg gct tct tcc act gtt gct gcg ccg tac gag ttc ccg acg ctg acg       48
Met Ala Ser Ser Thr Val Ala Ala Pro Tyr Glu Phe Pro Thr Leu Thr         
1               5                   10                  15              

gag atc aag cgc tcg ctg cca gcg cac tgc ttt gag gcc tcg gtc ccg       96
Glu Ile Lys Arg Ser Leu Pro Ala His Cys Phe Glu Ala Ser Val Pro         
            20                  25                  30                  

tgg tcg ctc tac tac acc gtg cgc gcg ctg ggc atc gcc ggc tcg ctc      144
Trp Ser Leu Tyr Tyr Thr Val Arg Ala Leu Gly Ile Ala Gly Ser Leu         
        35                  40                  45                      

gcg ctc ggc ctc tac tac gcg cgc gcg ctc gcg atc gtg cag gag ttt      192
Ala Leu Gly Leu Tyr Tyr Ala Arg Ala Leu Ala Ile Val Gln Glu Phe         
    50                  55                  60                          

gcc ctg ctg gat gcg gtg ctc tgc acg ggg tac att ctg ctg cag ggc      240
Ala Leu Leu Asp Ala Val Leu Cys Thr Gly Tyr Ile Leu Leu Gln Gly         
65                  70                  75                  80          

atc gta ttc tgg ggg ttc ttc acc atc ggc cat gac tgc ggc cac ggc      288
Ile Val Phe Trp Gly Phe Phe Thr Ile Gly His Asp Cys Gly His Gly         
                85                  90                  95              

gcg ttc tcg cgt tcg cac ctg ctc aac ttc agc gtc ggc acg ctc att      336
Ala Phe Ser Arg Ser His Leu Leu Asn Phe Ser Val Gly Thr Leu Ile         
            100                 105                 110                 

cac tcg atc atc ctc acg ccg tac gag tca tgg aag atc tcg cac cgc      384
His Ser Ile Ile Leu Thr Pro Tyr Glu Ser Trp Lys Ile Ser His Arg         
        115                 120                 125                     

cac cac cac aag aac acg ggc aac atc gac aag gac gag att ttc tac      432
His His His Lys Asn Thr Gly Asn Ile Asp Lys Asp Glu Ile Phe Tyr         
    130                 135                 140                         

ccg cag cgc gag gcc gac tcg cac cca ctg tcc cga cac atg gtg atc      480
Pro Gln Arg Glu Ala Asp Ser His Pro Leu Ser Arg His Met Val Ile         
145                 150                 155                 160         

tcg ctc ggc tcg gcc tgg ttc gcg tac ctc gtt gcg ggc ttc cct cct      528
Ser Leu Gly Ser Ala Trp Phe Ala Tyr Leu Val Ala Gly Phe Pro Pro         
                165                 170                 175             

cgc aag gtg aac cac ttc aac cct tgg gaa ccg ttg tac ctg cgc cgc      576
Arg Lys Val Asn His Phe Asn Pro Trp Glu Pro Leu Tyr Leu Arg Arg         
            180                 185                 190                 

atg tct gcc gtc atc atc tca ctc ggc tcg ctc gtg gcg ttc gcg ggc      624
Met Ser Ala Val Ile Ile Ser Leu Gly Ser Leu Val Ala Phe Ala Gly         
        195                 200                 205                     

ttg tat gcg tat ctc acc tac gtc tat ggc ctt aag acc atg gcg ctg      672
Leu Tyr Ala Tyr Leu Thr Tyr Val Tyr Gly Leu Lys Thr Met Ala Leu         
    210                 215                 220                         

tac tac ttc gcc cct ctc ttt ggg ttc gcc acg atg ctc gtg gtc act      720
Tyr Tyr Phe Ala Pro Leu Phe Gly Phe Ala Thr Met Leu Val Val Thr         
225                 230                 235                 240         

acc ttt ttg cac cac aat gac gag gaa acg cca tgg tac gcc gac tcg      768
Thr Phe Leu His His Asn Asp Glu Glu Thr Pro Trp Tyr Ala Asp Ser         
                245                 250                 255             

gag tgg acg tac gtc aag ggc aac ctc tcg tcc gtg gac cgc tcg tac      816
Glu Trp Thr Tyr Val Lys Gly Asn Leu Ser Ser Val Asp Arg Ser Tyr         
            260                 265                 270                 

ggc gcg ctc atc gac aac ctg agc cac aac atc ggc acg cac cag atc      864
Gly Ala Leu Ile Asp Asn Leu Ser His Asn Ile Gly Thr His Gln Ile         
        275                 280                 285                     

cac cac ctg ttt ccg atc atc ccg cac tac aag ctg aac gag gcg acg      912
His His Leu Phe Pro Ile Ile Pro His Tyr Lys Leu Asn Glu Ala Thr         
    290                 295                 300                         

gca gcg ttc gcg cag gcg ttc ccg gag ctc gtg cgc aag agc gcg tcg      960
Ala Ala Phe Ala Gln Ala Phe Pro Glu Leu Val Arg Lys Ser Ala Ser         
305                 310                 315                 320         

ccg atc atc ccg acg ttc atc cgc atc ggg ctc atg tac gcc aag tac     1008
Pro Ile Ile Pro Thr Phe Ile Arg Ile Gly Leu Met Tyr Ala Lys Tyr         
                325                 330                 335             

ggc gtc gtg gac aag gac gcc aag atg ttt acg ctc aag gag gcc aag     1056
Gly Val Val Asp Lys Asp Ala Lys Met Phe Thr Leu Lys Glu Ala Lys         
            340                 345                 350                 

gcc gcc aag acc aag gcc aac tag                                     1080
Ala Ala Lys Thr Lys Ala Asn                                             
        355                                                             


<210>  51
<211>  359
<212>  PRT
<213>  Pythium aphanidermatum  

<400>  51

Met Ala Ser Ser Thr Val Ala Ala Pro Tyr Glu Phe Pro Thr Leu Thr 
1               5                   10                  15      


Glu Ile Lys Arg Ser Leu Pro Ala His Cys Phe Glu Ala Ser Val Pro 
            20                  25                  30          


Trp Ser Leu Tyr Tyr Thr Val Arg Ala Leu Gly Ile Ala Gly Ser Leu 
        35                  40                  45              


Ala Leu Gly Leu Tyr Tyr Ala Arg Ala Leu Ala Ile Val Gln Glu Phe 
    50                  55                  60                  


Ala Leu Leu Asp Ala Val Leu Cys Thr Gly Tyr Ile Leu Leu Gln Gly 
65                  70                  75                  80  


Ile Val Phe Trp Gly Phe Phe Thr Ile Gly His Asp Cys Gly His Gly 
                85                  90                  95      


Ala Phe Ser Arg Ser His Leu Leu Asn Phe Ser Val Gly Thr Leu Ile 
            100                 105                 110         


His Ser Ile Ile Leu Thr Pro Tyr Glu Ser Trp Lys Ile Ser His Arg 
        115                 120                 125             


His His His Lys Asn Thr Gly Asn Ile Asp Lys Asp Glu Ile Phe Tyr 
    130                 135                 140                 


Pro Gln Arg Glu Ala Asp Ser His Pro Leu Ser Arg His Met Val Ile 
145                 150                 155                 160 


Ser Leu Gly Ser Ala Trp Phe Ala Tyr Leu Val Ala Gly Phe Pro Pro 
                165                 170                 175     


Arg Lys Val Asn His Phe Asn Pro Trp Glu Pro Leu Tyr Leu Arg Arg 
            180                 185                 190         


Met Ser Ala Val Ile Ile Ser Leu Gly Ser Leu Val Ala Phe Ala Gly 
        195                 200                 205             


Leu Tyr Ala Tyr Leu Thr Tyr Val Tyr Gly Leu Lys Thr Met Ala Leu 
    210                 215                 220                 


Tyr Tyr Phe Ala Pro Leu Phe Gly Phe Ala Thr Met Leu Val Val Thr 
225                 230                 235                 240 


Thr Phe Leu His His Asn Asp Glu Glu Thr Pro Trp Tyr Ala Asp Ser 
                245                 250                 255     


Glu Trp Thr Tyr Val Lys Gly Asn Leu Ser Ser Val Asp Arg Ser Tyr 
            260                 265                 270         


Gly Ala Leu Ile Asp Asn Leu Ser His Asn Ile Gly Thr His Gln Ile 
        275                 280                 285             


His His Leu Phe Pro Ile Ile Pro His Tyr Lys Leu Asn Glu Ala Thr 
    290                 295                 300                 


Ala Ala Phe Ala Gln Ala Phe Pro Glu Leu Val Arg Lys Ser Ala Ser 
305                 310                 315                 320 


Pro Ile Ile Pro Thr Phe Ile Arg Ile Gly Leu Met Tyr Ala Lys Tyr 
                325                 330                 335     


Gly Val Val Asp Lys Asp Ala Lys Met Phe Thr Leu Lys Glu Ala Lys 
            340                 345                 350         


Ala Ala Lys Thr Lys Ala Asn 
        355                 


<210>  52
<211>  1080
<212>  DNA
<213>  Pythium aphanidermatum 


<220>
<221>  CDS
<222>  (1)..(1080)
<223>  synthetic delta-17 desaturase (codon-optimized for Yarrowia 
       lipolytica)

<300>
<302>  DELTA-17 DESATURASES AND THEIR USE IN MAKING POLYUNSATURATED 
       FATTY ACIDS
<310>  WO 2008/054565
<311>  2007-07-19
<312>  2008-05-08
<313>  (1)..(1080)

<300>
<302>  DELTA-17 DESATURASES AND THEIR USE IN MAKING POLYUNSATURATED 
       FATTY ACIDS
<310>  US 2008-0125326-A1
<311>  2007-07-19
<312>  2008-05-29
<313>  (1)..(1080)

<400>  52
atg gct tcc tct acc gtt gcc gct ccc tac gag ttc cct act ctc acc       48
Met Ala Ser Ser Thr Val Ala Ala Pro Tyr Glu Phe Pro Thr Leu Thr         
1               5                   10                  15              

gag atc aag cga tcc ctg cct gcc cac tgc ttc gaa gcc tct gtt ccc       96
Glu Ile Lys Arg Ser Leu Pro Ala His Cys Phe Glu Ala Ser Val Pro         
            20                  25                  30                  

tgg tcc ctc tac tat acc gtg cga gct ctg ggc att gcc ggt tcc ctt      144
Trp Ser Leu Tyr Tyr Thr Val Arg Ala Leu Gly Ile Ala Gly Ser Leu         
        35                  40                  45                      

gct ctc gga ctg tac tat gct cga gcc ctt gct atc gtg cag gag ttt      192
Ala Leu Gly Leu Tyr Tyr Ala Arg Ala Leu Ala Ile Val Gln Glu Phe         
    50                  55                  60                          

gca ctg ctc gat gcc gtc ctt tgc act ggc tac att ctg ctc cag ggt      240
Ala Leu Leu Asp Ala Val Leu Cys Thr Gly Tyr Ile Leu Leu Gln Gly         
65                  70                  75                  80          

atc gtg ttc tgg gga ttc ttt acc atc ggt cac gac tgt gga cat ggt      288
Ile Val Phe Trp Gly Phe Phe Thr Ile Gly His Asp Cys Gly His Gly         
                85                  90                  95              

gcc ttc tcg cga tcc cac ctg ctc aac ttc tct gtt ggc aca ctc att      336
Ala Phe Ser Arg Ser His Leu Leu Asn Phe Ser Val Gly Thr Leu Ile         
            100                 105                 110                 

cac tcc atc att ctg act ccc tac gag tcg tgg aag atc agc cat cga      384
His Ser Ile Ile Leu Thr Pro Tyr Glu Ser Trp Lys Ile Ser His Arg         
        115                 120                 125                     

cac cat cac aag aac acc ggc aac atc gac aag gat gag atc ttc tac      432
His His His Lys Asn Thr Gly Asn Ile Asp Lys Asp Glu Ile Phe Tyr         
    130                 135                 140                         

cct cag cga gaa gcc gac tct cat ccc ctg tcc cga cac atg gtc atc      480
Pro Gln Arg Glu Ala Asp Ser His Pro Leu Ser Arg His Met Val Ile         
145                 150                 155                 160         

tcc ctt ggt tcg gct tgg ttt gcc tac ctc gtt gct gga ttt cct ccc      528
Ser Leu Gly Ser Ala Trp Phe Ala Tyr Leu Val Ala Gly Phe Pro Pro         
                165                 170                 175             

cga aag gtc aac cac ttc aat ccc tgg gag cct ctc tac ctg cga aga      576
Arg Lys Val Asn His Phe Asn Pro Trp Glu Pro Leu Tyr Leu Arg Arg         
            180                 185                 190                 

atg tct gcc gtc atc att tcc ctc ggc tct ctc gtg gcc ttt gct ggt      624
Met Ser Ala Val Ile Ile Ser Leu Gly Ser Leu Val Ala Phe Ala Gly         
        195                 200                 205                     

ctg tac gcc tac ctt acc tac gtc tac ggc ctc aag acc atg gct ctg      672
Leu Tyr Ala Tyr Leu Thr Tyr Val Tyr Gly Leu Lys Thr Met Ala Leu         
    210                 215                 220                         

tat tac ttc gca cct ctc ttt gga ttc gcc acc atg ctg gtt gtc act      720
Tyr Tyr Phe Ala Pro Leu Phe Gly Phe Ala Thr Met Leu Val Val Thr         
225                 230                 235                 240         

acc ttc ctc cat cac aac gac gag gaa act ccc tgg tac gcc gat tcg      768
Thr Phe Leu His His Asn Asp Glu Glu Thr Pro Trp Tyr Ala Asp Ser         
                245                 250                 255             

gag tgg acc tat gtc aag ggc aac ttg tcc tct gtg gac cga agc tac      816
Glu Trp Thr Tyr Val Lys Gly Asn Leu Ser Ser Val Asp Arg Ser Tyr         
            260                 265                 270                 

gga gcc ctc atc gac aac ctg tcc cac aac att ggt aca cat cag atc      864
Gly Ala Leu Ile Asp Asn Leu Ser His Asn Ile Gly Thr His Gln Ile         
        275                 280                 285                     

cac cat ctg ttt ccc atc att cct cac tac aag ctc aac gag gcc act      912
His His Leu Phe Pro Ile Ile Pro His Tyr Lys Leu Asn Glu Ala Thr         
    290                 295                 300                         

gct gcc ttc gct cag gcc ttt ccc gaa ctg gtg cga aag tcg gct tct      960
Ala Ala Phe Ala Gln Ala Phe Pro Glu Leu Val Arg Lys Ser Ala Ser         
305                 310                 315                 320         

ccc atc att ccc acc ttc atc cga att ggt ctt atg tac gcc aag tac     1008
Pro Ile Ile Pro Thr Phe Ile Arg Ile Gly Leu Met Tyr Ala Lys Tyr         
                325                 330                 335             

ggc gtg gtc gac aag gat gcc aag atg ttt acc ctc aag gag gcc aag     1056
Gly Val Val Asp Lys Asp Ala Lys Met Phe Thr Leu Lys Glu Ala Lys         
            340                 345                 350                 

gct gcc aag acc aaa gcc aac taa                                     1080
Ala Ala Lys Thr Lys Ala Asn                                             
        355                                                             


<210>  53
<211>  359
<212>  PRT
<213>  Pythium aphanidermatum 

<400>  53

Met Ala Ser Ser Thr Val Ala Ala Pro Tyr Glu Phe Pro Thr Leu Thr 
1               5                   10                  15      


Glu Ile Lys Arg Ser Leu Pro Ala His Cys Phe Glu Ala Ser Val Pro 
            20                  25                  30          


Trp Ser Leu Tyr Tyr Thr Val Arg Ala Leu Gly Ile Ala Gly Ser Leu 
        35                  40                  45              


Ala Leu Gly Leu Tyr Tyr Ala Arg Ala Leu Ala Ile Val Gln Glu Phe 
    50                  55                  60                  


Ala Leu Leu Asp Ala Val Leu Cys Thr Gly Tyr Ile Leu Leu Gln Gly 
65                  70                  75                  80  


Ile Val Phe Trp Gly Phe Phe Thr Ile Gly His Asp Cys Gly His Gly 
                85                  90                  95      


Ala Phe Ser Arg Ser His Leu Leu Asn Phe Ser Val Gly Thr Leu Ile 
            100                 105                 110         


His Ser Ile Ile Leu Thr Pro Tyr Glu Ser Trp Lys Ile Ser His Arg 
        115                 120                 125             


His His His Lys Asn Thr Gly Asn Ile Asp Lys Asp Glu Ile Phe Tyr 
    130                 135                 140                 


Pro Gln Arg Glu Ala Asp Ser His Pro Leu Ser Arg His Met Val Ile 
145                 150                 155                 160 


Ser Leu Gly Ser Ala Trp Phe Ala Tyr Leu Val Ala Gly Phe Pro Pro 
                165                 170                 175     


Arg Lys Val Asn His Phe Asn Pro Trp Glu Pro Leu Tyr Leu Arg Arg 
            180                 185                 190         


Met Ser Ala Val Ile Ile Ser Leu Gly Ser Leu Val Ala Phe Ala Gly 
        195                 200                 205             


Leu Tyr Ala Tyr Leu Thr Tyr Val Tyr Gly Leu Lys Thr Met Ala Leu 
    210                 215                 220                 


Tyr Tyr Phe Ala Pro Leu Phe Gly Phe Ala Thr Met Leu Val Val Thr 
225                 230                 235                 240 


Thr Phe Leu His His Asn Asp Glu Glu Thr Pro Trp Tyr Ala Asp Ser 
                245                 250                 255     


Glu Trp Thr Tyr Val Lys Gly Asn Leu Ser Ser Val Asp Arg Ser Tyr 
            260                 265                 270         


Gly Ala Leu Ile Asp Asn Leu Ser His Asn Ile Gly Thr His Gln Ile 
        275                 280                 285             


His His Leu Phe Pro Ile Ile Pro His Tyr Lys Leu Asn Glu Ala Thr 
    290                 295                 300                 


Ala Ala Phe Ala Gln Ala Phe Pro Glu Leu Val Arg Lys Ser Ala Ser 
305                 310                 315                 320 


Pro Ile Ile Pro Thr Phe Ile Arg Ile Gly Leu Met Tyr Ala Lys Tyr 
                325                 330                 335     


Gly Val Val Asp Lys Asp Ala Lys Met Phe Thr Leu Lys Glu Ala Lys 
            340                 345                 350         


Ala Ala Lys Thr Lys Ala Asn 
        355                 


<210>  54
<211>  1434
<212>  DNA
<213>  Fusarium monoliforme


<220>
<221>  CDS
<222>  (1)..(1434)
<223>  delta-12 desaturase

<300>
<302>  DELTA-12 DESATURASES SUITABLE FOR ALTERING LEVELS OF 
       POLYUNSATURATED FATTY ACIDS IN OLEAGINOUS YEAST
<310>  WO 2005/047485
<311>  2004-11-12
<312>  2005-05-26
<313>  (1)..(1434)

<300>
<302>  DELTA-12 DESATURASES SUITABLE FOR ALTERING LEVELS OF 
       POLYUNSATURATED FATTY ACIDS IN OLEAGINOUS YEAST
<310>  US 2005-0216975-A1
<311>  2004-11-10
<312>  2005-09-29
<313>  (1)..(1434)

<400>  54
atg gcg tcc act tcg gct ctg ccc aag cag aac cct gcg ctt aga cgc       48
Met Ala Ser Thr Ser Ala Leu Pro Lys Gln Asn Pro Ala Leu Arg Arg         
1               5                   10                  15              

acc gtc acc tca act act gtg acg gat tct gag tct gcc gcc gtc tct       96
Thr Val Thr Ser Thr Thr Val Thr Asp Ser Glu Ser Ala Ala Val Ser         
            20                  25                  30                  

cct tca gac tct ccc cgc cac tcg gcc tct tcc aca tcg ctc tcg tcc      144
Pro Ser Asp Ser Pro Arg His Ser Ala Ser Ser Thr Ser Leu Ser Ser         
        35                  40                  45                      

atg tcc gag gtt gat atc gcc aag ccc aag tcc gag tat ggt gtc atg      192
Met Ser Glu Val Asp Ile Ala Lys Pro Lys Ser Glu Tyr Gly Val Met         
    50                  55                  60                          

ctc gac acc tac ggc aac cag ttc gag gtt ccc gac ttt acc atc aag      240
Leu Asp Thr Tyr Gly Asn Gln Phe Glu Val Pro Asp Phe Thr Ile Lys         
65                  70                  75                  80          

gac atc tac aat gcc atc cct aag cac tgc ttc aag cgc tcc gct ctc      288
Asp Ile Tyr Asn Ala Ile Pro Lys His Cys Phe Lys Arg Ser Ala Leu         
                85                  90                  95              

aag gga tac ggt tat atc ctc cgc gac att gtc ctc ctg act acc act      336
Lys Gly Tyr Gly Tyr Ile Leu Arg Asp Ile Val Leu Leu Thr Thr Thr         
            100                 105                 110                 

ttc agc atc tgg tac aac ttt gtg acc ccc gaa tat atc ccc tcc acc      384
Phe Ser Ile Trp Tyr Asn Phe Val Thr Pro Glu Tyr Ile Pro Ser Thr         
        115                 120                 125                     

ccc gcc cgc gct ggt ctg tgg gcc gtg tac acc gtt ctt cag ggt ctt      432
Pro Ala Arg Ala Gly Leu Trp Ala Val Tyr Thr Val Leu Gln Gly Leu         
    130                 135                 140                         

ttc ggt act ggt ctc tgg gtt att gcc cat gag tgc ggt cac ggt gct      480
Phe Gly Thr Gly Leu Trp Val Ile Ala His Glu Cys Gly His Gly Ala         
145                 150                 155                 160         

ttc tcc gat tct cgc atc atc aac gac att act ggc tgg gtt ctt cac      528
Phe Ser Asp Ser Arg Ile Ile Asn Asp Ile Thr Gly Trp Val Leu His         
                165                 170                 175             

tct tcc ctc ctt gtc ccc tac ttc agc tgg caa atc tcc cac cga aag      576
Ser Ser Leu Leu Val Pro Tyr Phe Ser Trp Gln Ile Ser His Arg Lys         
            180                 185                 190                 

cac cac aag gcc act ggc aac atg gag cgt gac atg gtc ttc gtt ccc      624
His His Lys Ala Thr Gly Asn Met Glu Arg Asp Met Val Phe Val Pro         
        195                 200                 205                     

cga acc cgc gag cag cag gct act cgt ctc gga aag atg acc cac gag      672
Arg Thr Arg Glu Gln Gln Ala Thr Arg Leu Gly Lys Met Thr His Glu         
    210                 215                 220                         

ctc gct cat ctt act gag gag acc ccc gct ttc act ctt ctc atg ctc      720
Leu Ala His Leu Thr Glu Glu Thr Pro Ala Phe Thr Leu Leu Met Leu         
225                 230                 235                 240         

gtc ctt cag cag ctc gtt ggc tgg ccc aac tac ctc atc acc aat gtt      768
Val Leu Gln Gln Leu Val Gly Trp Pro Asn Tyr Leu Ile Thr Asn Val         
                245                 250                 255             

acc ggc cac aac tac cac gag cgc cag cgt gag ggt cgc ggc aag ggc      816
Thr Gly His Asn Tyr His Glu Arg Gln Arg Glu Gly Arg Gly Lys Gly         
            260                 265                 270                 

aag cat aac ggc ctc ggc ggt ggt gtt aac cac ttc gat ccc cgc agc      864
Lys His Asn Gly Leu Gly Gly Gly Val Asn His Phe Asp Pro Arg Ser         
        275                 280                 285                     

cct ctg tac gag aac agt gac gct aag ctc atc gtc ctc agc gat att      912
Pro Leu Tyr Glu Asn Ser Asp Ala Lys Leu Ile Val Leu Ser Asp Ile         
    290                 295                 300                         

ggt atc ggt ctg atg gcc act gct ctg tac ttc ctc gtt cag aag ttc      960
Gly Ile Gly Leu Met Ala Thr Ala Leu Tyr Phe Leu Val Gln Lys Phe         
305                 310                 315                 320         

ggt ttc tac aac atg gcc atc tgg tac ttt gtt ccc tac ctc tgg gtt     1008
Gly Phe Tyr Asn Met Ala Ile Trp Tyr Phe Val Pro Tyr Leu Trp Val         
                325                 330                 335             

aac cac tgg ctc gtt gcc atc acc ttc ctc cag cac acc gac cct acc     1056
Asn His Trp Leu Val Ala Ile Thr Phe Leu Gln His Thr Asp Pro Thr         
            340                 345                 350                 

ctt ccc cac tac acc aac gac gag tgg aac ttc gtc cgt ggt gcc gct     1104
Leu Pro His Tyr Thr Asn Asp Glu Trp Asn Phe Val Arg Gly Ala Ala         
        355                 360                 365                     

gct acc att gac cgt gag atg ggc ttc atc ggc cgc cac ctt ctc cac     1152
Ala Thr Ile Asp Arg Glu Met Gly Phe Ile Gly Arg His Leu Leu His         
    370                 375                 380                         

ggc atc atc gag act cat gtc ctc cac cac tac gtc agc agc atc ccc     1200
Gly Ile Ile Glu Thr His Val Leu His His Tyr Val Ser Ser Ile Pro         
385                 390                 395                 400         

ttc tac aac gcg gac gag gcc acc gag gcc att aag ccc atc atg ggc     1248
Phe Tyr Asn Ala Asp Glu Ala Thr Glu Ala Ile Lys Pro Ile Met Gly         
                405                 410                 415             

aag cac tac cgg gct gat gtc cag gat ggt cct cgt ggc ttc atc cgc     1296
Lys His Tyr Arg Ala Asp Val Gln Asp Gly Pro Arg Gly Phe Ile Arg         
            420                 425                 430                 

gcc atg tac cgc agt gcg cgt atg tgc cag tgg gtt gag ccc agc gct     1344
Ala Met Tyr Arg Ser Ala Arg Met Cys Gln Trp Val Glu Pro Ser Ala         
        435                 440                 445                     

ggt gcc gag ggt gct ggt aag ggt gtt ctg ttc ttc cgc aac cgc aac     1392
Gly Ala Glu Gly Ala Gly Lys Gly Val Leu Phe Phe Arg Asn Arg Asn         
    450                 455                 460                         

aac gtg ggc acc ccc ccc gct gtt atc aag ccc gtt gct taa             1434
Asn Val Gly Thr Pro Pro Ala Val Ile Lys Pro Val Ala                     
465                 470                 475                             


<210>  55
<211>  477
<212>  PRT
<213>  Fusarium monoliforme

<400>  55

Met Ala Ser Thr Ser Ala Leu Pro Lys Gln Asn Pro Ala Leu Arg Arg 
1               5                   10                  15      


Thr Val Thr Ser Thr Thr Val Thr Asp Ser Glu Ser Ala Ala Val Ser 
            20                  25                  30          


Pro Ser Asp Ser Pro Arg His Ser Ala Ser Ser Thr Ser Leu Ser Ser 
        35                  40                  45              


Met Ser Glu Val Asp Ile Ala Lys Pro Lys Ser Glu Tyr Gly Val Met 
    50                  55                  60                  


Leu Asp Thr Tyr Gly Asn Gln Phe Glu Val Pro Asp Phe Thr Ile Lys 
65                  70                  75                  80  


Asp Ile Tyr Asn Ala Ile Pro Lys His Cys Phe Lys Arg Ser Ala Leu 
                85                  90                  95      


Lys Gly Tyr Gly Tyr Ile Leu Arg Asp Ile Val Leu Leu Thr Thr Thr 
            100                 105                 110         


Phe Ser Ile Trp Tyr Asn Phe Val Thr Pro Glu Tyr Ile Pro Ser Thr 
        115                 120                 125             


Pro Ala Arg Ala Gly Leu Trp Ala Val Tyr Thr Val Leu Gln Gly Leu 
    130                 135                 140                 


Phe Gly Thr Gly Leu Trp Val Ile Ala His Glu Cys Gly His Gly Ala 
145                 150                 155                 160 


Phe Ser Asp Ser Arg Ile Ile Asn Asp Ile Thr Gly Trp Val Leu His 
                165                 170                 175     


Ser Ser Leu Leu Val Pro Tyr Phe Ser Trp Gln Ile Ser His Arg Lys 
            180                 185                 190         


His His Lys Ala Thr Gly Asn Met Glu Arg Asp Met Val Phe Val Pro 
        195                 200                 205             


Arg Thr Arg Glu Gln Gln Ala Thr Arg Leu Gly Lys Met Thr His Glu 
    210                 215                 220                 


Leu Ala His Leu Thr Glu Glu Thr Pro Ala Phe Thr Leu Leu Met Leu 
225                 230                 235                 240 


Val Leu Gln Gln Leu Val Gly Trp Pro Asn Tyr Leu Ile Thr Asn Val 
                245                 250                 255     


Thr Gly His Asn Tyr His Glu Arg Gln Arg Glu Gly Arg Gly Lys Gly 
            260                 265                 270         


Lys His Asn Gly Leu Gly Gly Gly Val Asn His Phe Asp Pro Arg Ser 
        275                 280                 285             


Pro Leu Tyr Glu Asn Ser Asp Ala Lys Leu Ile Val Leu Ser Asp Ile 
    290                 295                 300                 


Gly Ile Gly Leu Met Ala Thr Ala Leu Tyr Phe Leu Val Gln Lys Phe 
305                 310                 315                 320 


Gly Phe Tyr Asn Met Ala Ile Trp Tyr Phe Val Pro Tyr Leu Trp Val 
                325                 330                 335     


Asn His Trp Leu Val Ala Ile Thr Phe Leu Gln His Thr Asp Pro Thr 
            340                 345                 350         


Leu Pro His Tyr Thr Asn Asp Glu Trp Asn Phe Val Arg Gly Ala Ala 
        355                 360                 365             


Ala Thr Ile Asp Arg Glu Met Gly Phe Ile Gly Arg His Leu Leu His 
    370                 375                 380                 


Gly Ile Ile Glu Thr His Val Leu His His Tyr Val Ser Ser Ile Pro 
385                 390                 395                 400 


Phe Tyr Asn Ala Asp Glu Ala Thr Glu Ala Ile Lys Pro Ile Met Gly 
                405                 410                 415     


Lys His Tyr Arg Ala Asp Val Gln Asp Gly Pro Arg Gly Phe Ile Arg 
            420                 425                 430         


Ala Met Tyr Arg Ser Ala Arg Met Cys Gln Trp Val Glu Pro Ser Ala 
        435                 440                 445             


Gly Ala Glu Gly Ala Gly Lys Gly Val Leu Phe Phe Arg Asn Arg Asn 
    450                 455                 460                 


Asn Val Gly Thr Pro Pro Ala Val Ile Lys Pro Val Ala 
465                 470                 475         


<210>  56
<211>  1434
<212>  DNA
<213>  Fusarium moniliforme 


<220>
<221>  CDS
<222>  (1)..(1434)
<223>  synthetic delta-12 desaturase (codon-optimized for Yarrowia 
       lipolytica)

<300>
<302>  DELTA-12 DESATURASES SUITABLE FOR ALTERING LEVELS OF 
       POLYUNSATURATED FATTY ACIDS IN OLEAGINOUS YEAST
<310>  WO 2005/047485
<311>  2004-11-12
<312>  2005-05-26
<313>  (1)..(1434)

<300>
<302>  DELTA-12 DESATURASES SUITABLE FOR ALTERING LEVELS OF 
       POLYUNSATURATED FATTY ACIDS IN OLEAGINOUS YEAST
<310>  US 2005-0216975-A1
<311>  2004-11-10
<312>  2005-09-29
<313>  (1)..(1434)

<400>  56
atg gcc tcc acc tcg gct ctg ccc aag cag aac cct gcc ctc cga cga       48
Met Ala Ser Thr Ser Ala Leu Pro Lys Gln Asn Pro Ala Leu Arg Arg         
1               5                   10                  15              

acc gtc act tcc acc act gtg acc gac tcg gag tct gct gcc gtc tct       96
Thr Val Thr Ser Thr Thr Val Thr Asp Ser Glu Ser Ala Ala Val Ser         
            20                  25                  30                  

ccc tcc gat tct ccc aga cac tcg gcc tcc tct aca tcg ctg tct tcc      144
Pro Ser Asp Ser Pro Arg His Ser Ala Ser Ser Thr Ser Leu Ser Ser         
        35                  40                  45                      

atg tcc gag gtg gac att gcc aag ccc aag tcc gag tac ggt gtc atg      192
Met Ser Glu Val Asp Ile Ala Lys Pro Lys Ser Glu Tyr Gly Val Met         
    50                  55                  60                          

ctg gat acc tac ggc aac cag ttc gaa gtt ccc gac ttc acc atc aag      240
Leu Asp Thr Tyr Gly Asn Gln Phe Glu Val Pro Asp Phe Thr Ile Lys         
65                  70                  75                  80          

gac atc tac aac gct att ccc aag cac tgc ttc aag cga tct gct ctc      288
Asp Ile Tyr Asn Ala Ile Pro Lys His Cys Phe Lys Arg Ser Ala Leu         
                85                  90                  95              

aag gga tac ggc tac att ctt cga gac att gtc ctc ctg act acc act      336
Lys Gly Tyr Gly Tyr Ile Leu Arg Asp Ile Val Leu Leu Thr Thr Thr         
            100                 105                 110                 

ttc agc atc tgg tac aac ttt gtg aca ccc gag tac att ccc tcc act      384
Phe Ser Ile Trp Tyr Asn Phe Val Thr Pro Glu Tyr Ile Pro Ser Thr         
        115                 120                 125                     

cct gct cga gcc ggt ctg tgg gct gtg tac acc gtt ctt cag gga ctc      432
Pro Ala Arg Ala Gly Leu Trp Ala Val Tyr Thr Val Leu Gln Gly Leu         
    130                 135                 140                         

ttc ggt act gga ctg tgg gtc att gcc cac gag tgt gga cat ggt gct      480
Phe Gly Thr Gly Leu Trp Val Ile Ala His Glu Cys Gly His Gly Ala         
145                 150                 155                 160         

ttc tcc gat tcc cga atc atc aac gac att act ggc tgg gtg ctt cac      528
Phe Ser Asp Ser Arg Ile Ile Asn Asp Ile Thr Gly Trp Val Leu His         
                165                 170                 175             

tct tcc ctg ctt gtt ccc tac ttc agc tgg caa atc tcc cac cgg aag      576
Ser Ser Leu Leu Val Pro Tyr Phe Ser Trp Gln Ile Ser His Arg Lys         
            180                 185                 190                 

cat cac aag gcc act gga aac atg gag cga gac atg gtc ttc gtt cct      624
His His Lys Ala Thr Gly Asn Met Glu Arg Asp Met Val Phe Val Pro         
        195                 200                 205                     

cga acc cga gag cag caa gct act cga ctc ggc aag atg acc cac gaa      672
Arg Thr Arg Glu Gln Gln Ala Thr Arg Leu Gly Lys Met Thr His Glu         
    210                 215                 220                         

ctc gcc cat ctt acc gag gaa act cct gct ttc acc ctg ctc atg ctt      720
Leu Ala His Leu Thr Glu Glu Thr Pro Ala Phe Thr Leu Leu Met Leu         
225                 230                 235                 240         

gtg ctt cag caa ctg gtc ggt tgg ccc aac tat ctc att acc aac gtt      768
Val Leu Gln Gln Leu Val Gly Trp Pro Asn Tyr Leu Ile Thr Asn Val         
                245                 250                 255             

act gga cac aac tac cat gag cgg cag cga gag ggt cga ggc aag gga      816
Thr Gly His Asn Tyr His Glu Arg Gln Arg Glu Gly Arg Gly Lys Gly         
            260                 265                 270                 

aag cac aac ggt ctt ggc ggt gga gtt aac cat ttc gat ccc cga tct      864
Lys His Asn Gly Leu Gly Gly Gly Val Asn His Phe Asp Pro Arg Ser         
        275                 280                 285                     

cct ctg tac gag aac agc gac gcc aag ctc atc gtg ctc tcc gac att      912
Pro Leu Tyr Glu Asn Ser Asp Ala Lys Leu Ile Val Leu Ser Asp Ile         
    290                 295                 300                         

ggc att ggt ctt atg gcc acc gct ctg tac ttt ctc gtt cag aag ttc      960
Gly Ile Gly Leu Met Ala Thr Ala Leu Tyr Phe Leu Val Gln Lys Phe         
305                 310                 315                 320         

gga ttc tac aac atg gcc atc tgg tac ttc gtt ccc tac ttg tgg gtt     1008
Gly Phe Tyr Asn Met Ala Ile Trp Tyr Phe Val Pro Tyr Leu Trp Val         
                325                 330                 335             

aac cac tgg ctc gtc gcc att acc ttt ctg cag cac aca gat cct act     1056
Asn His Trp Leu Val Ala Ile Thr Phe Leu Gln His Thr Asp Pro Thr         
            340                 345                 350                 

ctt ccc cac tac acc aac gac gag tgg aac ttt gtg cga ggt gcc gct     1104
Leu Pro His Tyr Thr Asn Asp Glu Trp Asn Phe Val Arg Gly Ala Ala         
        355                 360                 365                     

gca acc atc gac cga gag atg ggc ttc att gga cgt cat ctg ctc cac     1152
Ala Thr Ile Asp Arg Glu Met Gly Phe Ile Gly Arg His Leu Leu His         
    370                 375                 380                         

ggc att atc gag act cac gtc ctg cat cac tac gtc tct tcc att ccc     1200
Gly Ile Ile Glu Thr His Val Leu His His Tyr Val Ser Ser Ile Pro         
385                 390                 395                 400         

ttc tac aat gcg gac gaa gct acc gag gcc atc aaa cct atc atg ggc     1248
Phe Tyr Asn Ala Asp Glu Ala Thr Glu Ala Ile Lys Pro Ile Met Gly         
                405                 410                 415             

aag cac tat cga gct gat gtc cag gac ggt cct cga gga ttc att cga     1296
Lys His Tyr Arg Ala Asp Val Gln Asp Gly Pro Arg Gly Phe Ile Arg         
            420                 425                 430                 

gcc atg tac cga tct gca cga atg tgc cag tgg gtt gaa ccc tcc gct     1344
Ala Met Tyr Arg Ser Ala Arg Met Cys Gln Trp Val Glu Pro Ser Ala         
        435                 440                 445                     

ggt gcc gag gga gct ggc aag ggt gtc ctg ttc ttt cga aac cga aac     1392
Gly Ala Glu Gly Ala Gly Lys Gly Val Leu Phe Phe Arg Asn Arg Asn         
    450                 455                 460                         

aat gtg ggc act cct ccc gct gtc atc aag ccc gtt gcc taa             1434
Asn Val Gly Thr Pro Pro Ala Val Ile Lys Pro Val Ala                     
465                 470                 475                             


<210>  57
<211>  477
<212>  PRT
<213>  Fusarium moniliforme 

<400>  57

Met Ala Ser Thr Ser Ala Leu Pro Lys Gln Asn Pro Ala Leu Arg Arg 
1               5                   10                  15      


Thr Val Thr Ser Thr Thr Val Thr Asp Ser Glu Ser Ala Ala Val Ser 
            20                  25                  30          


Pro Ser Asp Ser Pro Arg His Ser Ala Ser Ser Thr Ser Leu Ser Ser 
        35                  40                  45              


Met Ser Glu Val Asp Ile Ala Lys Pro Lys Ser Glu Tyr Gly Val Met 
    50                  55                  60                  


Leu Asp Thr Tyr Gly Asn Gln Phe Glu Val Pro Asp Phe Thr Ile Lys 
65                  70                  75                  80  


Asp Ile Tyr Asn Ala Ile Pro Lys His Cys Phe Lys Arg Ser Ala Leu 
                85                  90                  95      


Lys Gly Tyr Gly Tyr Ile Leu Arg Asp Ile Val Leu Leu Thr Thr Thr 
            100                 105                 110         


Phe Ser Ile Trp Tyr Asn Phe Val Thr Pro Glu Tyr Ile Pro Ser Thr 
        115                 120                 125             


Pro Ala Arg Ala Gly Leu Trp Ala Val Tyr Thr Val Leu Gln Gly Leu 
    130                 135                 140                 


Phe Gly Thr Gly Leu Trp Val Ile Ala His Glu Cys Gly His Gly Ala 
145                 150                 155                 160 


Phe Ser Asp Ser Arg Ile Ile Asn Asp Ile Thr Gly Trp Val Leu His 
                165                 170                 175     


Ser Ser Leu Leu Val Pro Tyr Phe Ser Trp Gln Ile Ser His Arg Lys 
            180                 185                 190         


His His Lys Ala Thr Gly Asn Met Glu Arg Asp Met Val Phe Val Pro 
        195                 200                 205             


Arg Thr Arg Glu Gln Gln Ala Thr Arg Leu Gly Lys Met Thr His Glu 
    210                 215                 220                 


Leu Ala His Leu Thr Glu Glu Thr Pro Ala Phe Thr Leu Leu Met Leu 
225                 230                 235                 240 


Val Leu Gln Gln Leu Val Gly Trp Pro Asn Tyr Leu Ile Thr Asn Val 
                245                 250                 255     


Thr Gly His Asn Tyr His Glu Arg Gln Arg Glu Gly Arg Gly Lys Gly 
            260                 265                 270         


Lys His Asn Gly Leu Gly Gly Gly Val Asn His Phe Asp Pro Arg Ser 
        275                 280                 285             


Pro Leu Tyr Glu Asn Ser Asp Ala Lys Leu Ile Val Leu Ser Asp Ile 
    290                 295                 300                 


Gly Ile Gly Leu Met Ala Thr Ala Leu Tyr Phe Leu Val Gln Lys Phe 
305                 310                 315                 320 


Gly Phe Tyr Asn Met Ala Ile Trp Tyr Phe Val Pro Tyr Leu Trp Val 
                325                 330                 335     


Asn His Trp Leu Val Ala Ile Thr Phe Leu Gln His Thr Asp Pro Thr 
            340                 345                 350         


Leu Pro His Tyr Thr Asn Asp Glu Trp Asn Phe Val Arg Gly Ala Ala 
        355                 360                 365             


Ala Thr Ile Asp Arg Glu Met Gly Phe Ile Gly Arg His Leu Leu His 
    370                 375                 380                 


Gly Ile Ile Glu Thr His Val Leu His His Tyr Val Ser Ser Ile Pro 
385                 390                 395                 400 


Phe Tyr Asn Ala Asp Glu Ala Thr Glu Ala Ile Lys Pro Ile Met Gly 
                405                 410                 415     


Lys His Tyr Arg Ala Asp Val Gln Asp Gly Pro Arg Gly Phe Ile Arg 
            420                 425                 430         


Ala Met Tyr Arg Ser Ala Arg Met Cys Gln Trp Val Glu Pro Ser Ala 
        435                 440                 445             


Gly Ala Glu Gly Ala Gly Lys Gly Val Leu Phe Phe Arg Asn Arg Asn 
    450                 455                 460                 


Asn Val Gly Thr Pro Pro Ala Val Ile Lys Pro Val Ala 
465                 470                 475         


<210>  58
<211>  828
<212>  DNA
<213>  Mortierella alpina


<220>
<221>  CDS
<222>  (1)..(828)
<223>  C16/18 elongase

<300>
<302>  A MORTIERELLA ALPINA C16/18 FATTY ACID ELONGASE
<310>  WO 2007/046817
<311>  2005-11-04
<312>  2007-04-26
<313>  (1)..(828)

<300>
<302>  A MORTIERELLA ALPINA C16/18 FATTY ACID ELONGASE
<310>  US 2007-0087420-A1
<311>  2005-10-19
<312>  2007-04-19
<313>  (1)..(828)

<400>  58
atg gag tct gga cct atg cct gcc ggg atc ccc ttc cct gaa tac tat       48
Met Glu Ser Gly Pro Met Pro Ala Gly Ile Pro Phe Pro Glu Tyr Tyr         
1               5                   10                  15              

gac ttt ttc atg gac tgg aag aca ccc ctg gca att gct gcc acc tac       96
Asp Phe Phe Met Asp Trp Lys Thr Pro Leu Ala Ile Ala Ala Thr Tyr         
            20                  25                  30                  

acc gcc gct gtt ggg ctc ttc aac ccc aag gtt ggc aaa gtc tcg cgc      144
Thr Ala Ala Val Gly Leu Phe Asn Pro Lys Val Gly Lys Val Ser Arg         
        35                  40                  45                      

gtg gta gcc aag tcg gct aac gcc aag ccg gca gag cgc acg cag tcc      192
Val Val Ala Lys Ser Ala Asn Ala Lys Pro Ala Glu Arg Thr Gln Ser         
    50                  55                  60                          

ggc gcc gcc atg acc gcc ttt gtc ttt gtc cac aac ctt atc ctc tgc      240
Gly Ala Ala Met Thr Ala Phe Val Phe Val His Asn Leu Ile Leu Cys         
65                  70                  75                  80          

gtg tac tct gga atc acc ttc tac tac atg ttc cca gcc atg gtc aag      288
Val Tyr Ser Gly Ile Thr Phe Tyr Tyr Met Phe Pro Ala Met Val Lys         
                85                  90                  95              

aac ttt aga aca cat acc ctc cat gag gcc tac tgc gat acg gat cag      336
Asn Phe Arg Thr His Thr Leu His Glu Ala Tyr Cys Asp Thr Asp Gln         
            100                 105                 110                 

agc ctg tgg aac aac gcc ctt ggc tac tgg ggc tac ctc ttc tac ctt      384
Ser Leu Trp Asn Asn Ala Leu Gly Tyr Trp Gly Tyr Leu Phe Tyr Leu         
        115                 120                 125                     

tca aag ttt tac gag gtc att gac acc atc atc atc atc ttg aag ggg      432
Ser Lys Phe Tyr Glu Val Ile Asp Thr Ile Ile Ile Ile Leu Lys Gly         
    130                 135                 140                         

cgc cgc tcg tcc ctg ctc cag acc tac cac cac gcc ggc gct atg atc      480
Arg Arg Ser Ser Leu Leu Gln Thr Tyr His His Ala Gly Ala Met Ile         
145                 150                 155                 160         

acc atg tgg tcc ggc atc aac tac cag gca acg ccc att tgg att ttt      528
Thr Met Trp Ser Gly Ile Asn Tyr Gln Ala Thr Pro Ile Trp Ile Phe         
                165                 170                 175             

gtc gtc ttc aac tcg ttc atc cac acc atc atg tac tgt tac tat gcc      576
Val Val Phe Asn Ser Phe Ile His Thr Ile Met Tyr Cys Tyr Tyr Ala         
            180                 185                 190                 

ttc acc tca atc ggc ttc cac ccc cca ggc aag aag tac ctc acc tcc      624
Phe Thr Ser Ile Gly Phe His Pro Pro Gly Lys Lys Tyr Leu Thr Ser         
        195                 200                 205                     

atg cag atc acc cag ttt ttg gtc ggc atc act atc gcc gtc tct tat      672
Met Gln Ile Thr Gln Phe Leu Val Gly Ile Thr Ile Ala Val Ser Tyr         
    210                 215                 220                         

ctc ttc gtc cct gga tgt atc cgc aca ccc ggt gct cag atg gct gtc      720
Leu Phe Val Pro Gly Cys Ile Arg Thr Pro Gly Ala Gln Met Ala Val         
225                 230                 235                 240         

tgg atc aac gtc gga tac ctc ttt ccc ctc act tat ctc ttt gtg gat      768
Trp Ile Asn Val Gly Tyr Leu Phe Pro Leu Thr Tyr Leu Phe Val Asp         
                245                 250                 255             

ttt gcc aag cgt act tac tcc aag cgt agt gcc atc gcc gct cag aag      816
Phe Ala Lys Arg Thr Tyr Ser Lys Arg Ser Ala Ile Ala Ala Gln Lys         
            260                 265                 270                 

aag gcc cag taa                                                      828
Lys Ala Gln                                                             
        275                                                             


<210>  59
<211>  275
<212>  PRT
<213>  Mortierella alpina

<400>  59

Met Glu Ser Gly Pro Met Pro Ala Gly Ile Pro Phe Pro Glu Tyr Tyr 
1               5                   10                  15      


Asp Phe Phe Met Asp Trp Lys Thr Pro Leu Ala Ile Ala Ala Thr Tyr 
            20                  25                  30          


Thr Ala Ala Val Gly Leu Phe Asn Pro Lys Val Gly Lys Val Ser Arg 
        35                  40                  45              


Val Val Ala Lys Ser Ala Asn Ala Lys Pro Ala Glu Arg Thr Gln Ser 
    50                  55                  60                  


Gly Ala Ala Met Thr Ala Phe Val Phe Val His Asn Leu Ile Leu Cys 
65                  70                  75                  80  


Val Tyr Ser Gly Ile Thr Phe Tyr Tyr Met Phe Pro Ala Met Val Lys 
                85                  90                  95      


Asn Phe Arg Thr His Thr Leu His Glu Ala Tyr Cys Asp Thr Asp Gln 
            100                 105                 110         


Ser Leu Trp Asn Asn Ala Leu Gly Tyr Trp Gly Tyr Leu Phe Tyr Leu 
        115                 120                 125             


Ser Lys Phe Tyr Glu Val Ile Asp Thr Ile Ile Ile Ile Leu Lys Gly 
    130                 135                 140                 


Arg Arg Ser Ser Leu Leu Gln Thr Tyr His His Ala Gly Ala Met Ile 
145                 150                 155                 160 


Thr Met Trp Ser Gly Ile Asn Tyr Gln Ala Thr Pro Ile Trp Ile Phe 
                165                 170                 175     


Val Val Phe Asn Ser Phe Ile His Thr Ile Met Tyr Cys Tyr Tyr Ala 
            180                 185                 190         


Phe Thr Ser Ile Gly Phe His Pro Pro Gly Lys Lys Tyr Leu Thr Ser 
        195                 200                 205             


Met Gln Ile Thr Gln Phe Leu Val Gly Ile Thr Ile Ala Val Ser Tyr 
    210                 215                 220                 


Leu Phe Val Pro Gly Cys Ile Arg Thr Pro Gly Ala Gln Met Ala Val 
225                 230                 235                 240 


Trp Ile Asn Val Gly Tyr Leu Phe Pro Leu Thr Tyr Leu Phe Val Asp 
                245                 250                 255     


Phe Ala Lys Arg Thr Tyr Ser Lys Arg Ser Ala Ile Ala Ala Gln Lys 
            260                 265                 270         


Lys Ala Gln 
        275 


<210>  60
<211>  828
<212>  DNA
<213>  Mortierella alpina


<220>
<221>  CDS
<222>  (1)..(828)
<223>  synthetic C16/18 elongase (codon-optimized for Yarrowia 
       lipolytica)

<300>
<302>  A MORTIERELLA ALPINA C16/18 FATTY ACID ELONGASE
<310>  US 2007-0087420-A1
<311>  2005-10-19
<312>  2007-04-19
<313>  (1)..(828)

<300>
<302>  A MORTIERELLA ALPINA C16/18 FATTY ACID ELONGASE
<310>  WO 2007/046817
<311>  2005-11-04
<312>  2007-04-26
<313>  (1)..(828)

<400>  60
atg gag tct gga ccc atg cct gct ggc att ccc ttc cct gag tac tat       48
Met Glu Ser Gly Pro Met Pro Ala Gly Ile Pro Phe Pro Glu Tyr Tyr         
1               5                   10                  15              

gac ttc ttt atg gac tgg aag act ccc ctg gcc atc gct gcc acc tac       96
Asp Phe Phe Met Asp Trp Lys Thr Pro Leu Ala Ile Ala Ala Thr Tyr         
            20                  25                  30                  

act gct gcc gtc ggt ctc ttc aac ccc aag gtt ggc aag gtc tcc cga      144
Thr Ala Ala Val Gly Leu Phe Asn Pro Lys Val Gly Lys Val Ser Arg         
        35                  40                  45                      

gtg gtt gcc aag tcg gct aac gca aag cct gcc gag cga acc cag tcc      192
Val Val Ala Lys Ser Ala Asn Ala Lys Pro Ala Glu Arg Thr Gln Ser         
    50                  55                  60                          

gga gct gcc atg act gcc ttc gtc ttt gtg cac aac ctc att ctg tgt      240
Gly Ala Ala Met Thr Ala Phe Val Phe Val His Asn Leu Ile Leu Cys         
65                  70                  75                  80          

gtc tac tct ggc atc acc ttc tac tac atg ttt cct gct atg gtc aag      288
Val Tyr Ser Gly Ile Thr Phe Tyr Tyr Met Phe Pro Ala Met Val Lys         
                85                  90                  95              

aac ttc cga acc cac aca ctg cac gaa gcc tac tgc gac acg gat cag      336
Asn Phe Arg Thr His Thr Leu His Glu Ala Tyr Cys Asp Thr Asp Gln         
            100                 105                 110                 

tcc ctc tgg aac aac gca ctt ggc tac tgg ggt tac ctc ttc tac ctg      384
Ser Leu Trp Asn Asn Ala Leu Gly Tyr Trp Gly Tyr Leu Phe Tyr Leu         
        115                 120                 125                     

tcc aag ttc tac gag gtc att gac acc atc atc atc atc ctg aag gga      432
Ser Lys Phe Tyr Glu Val Ile Asp Thr Ile Ile Ile Ile Leu Lys Gly         
    130                 135                 140                         

cga cgg tcc tcg ctg ctt cag acc tac cac cat gct gga gcc atg att      480
Arg Arg Ser Ser Leu Leu Gln Thr Tyr His His Ala Gly Ala Met Ile         
145                 150                 155                 160         

acc atg tgg tct ggc atc aac tac caa gcc act ccc att tgg atc ttt      528
Thr Met Trp Ser Gly Ile Asn Tyr Gln Ala Thr Pro Ile Trp Ile Phe         
                165                 170                 175             

gtg gtc ttc aac tcc ttc att cac acc atc atg tac tgt tac tat gcc      576
Val Val Phe Asn Ser Phe Ile His Thr Ile Met Tyr Cys Tyr Tyr Ala         
            180                 185                 190                 

ttc acc tct atc gga ttc cat cct cct ggc aaa aag tac ctg act tcg      624
Phe Thr Ser Ile Gly Phe His Pro Pro Gly Lys Lys Tyr Leu Thr Ser         
        195                 200                 205                     

atg cag att act cag ttt ctg gtc ggt atc acc att gcc gtg tcc tac      672
Met Gln Ile Thr Gln Phe Leu Val Gly Ile Thr Ile Ala Val Ser Tyr         
    210                 215                 220                         

ctc ttc gtt cct ggc tgc atc cga aca ccc ggt gct cag atg gct gtc      720
Leu Phe Val Pro Gly Cys Ile Arg Thr Pro Gly Ala Gln Met Ala Val         
225                 230                 235                 240         

tgg atc aac gtc ggc tac ctg ttt ccc ttg acc tat ctg ttc gtg gac      768
Trp Ile Asn Val Gly Tyr Leu Phe Pro Leu Thr Tyr Leu Phe Val Asp         
                245                 250                 255             

ttt gcc aag cga acc tac tcc aag cga tct gcc att gcc gct cag aaa      816
Phe Ala Lys Arg Thr Tyr Ser Lys Arg Ser Ala Ile Ala Ala Gln Lys         
            260                 265                 270                 

aag gct cag taa                                                      828
Lys Ala Gln                                                             
        275                                                             


<210>  61
<211>  275
<212>  PRT
<213>  Mortierella alpina

<400>  61

Met Glu Ser Gly Pro Met Pro Ala Gly Ile Pro Phe Pro Glu Tyr Tyr 
1               5                   10                  15      


Asp Phe Phe Met Asp Trp Lys Thr Pro Leu Ala Ile Ala Ala Thr Tyr 
            20                  25                  30          


Thr Ala Ala Val Gly Leu Phe Asn Pro Lys Val Gly Lys Val Ser Arg 
        35                  40                  45              


Val Val Ala Lys Ser Ala Asn Ala Lys Pro Ala Glu Arg Thr Gln Ser 
    50                  55                  60                  


Gly Ala Ala Met Thr Ala Phe Val Phe Val His Asn Leu Ile Leu Cys 
65                  70                  75                  80  


Val Tyr Ser Gly Ile Thr Phe Tyr Tyr Met Phe Pro Ala Met Val Lys 
                85                  90                  95      


Asn Phe Arg Thr His Thr Leu His Glu Ala Tyr Cys Asp Thr Asp Gln 
            100                 105                 110         


Ser Leu Trp Asn Asn Ala Leu Gly Tyr Trp Gly Tyr Leu Phe Tyr Leu 
        115                 120                 125             


Ser Lys Phe Tyr Glu Val Ile Asp Thr Ile Ile Ile Ile Leu Lys Gly 
    130                 135                 140                 


Arg Arg Ser Ser Leu Leu Gln Thr Tyr His His Ala Gly Ala Met Ile 
145                 150                 155                 160 


Thr Met Trp Ser Gly Ile Asn Tyr Gln Ala Thr Pro Ile Trp Ile Phe 
                165                 170                 175     


Val Val Phe Asn Ser Phe Ile His Thr Ile Met Tyr Cys Tyr Tyr Ala 
            180                 185                 190         


Phe Thr Ser Ile Gly Phe His Pro Pro Gly Lys Lys Tyr Leu Thr Ser 
        195                 200                 205             


Met Gln Ile Thr Gln Phe Leu Val Gly Ile Thr Ile Ala Val Ser Tyr 
    210                 215                 220                 


Leu Phe Val Pro Gly Cys Ile Arg Thr Pro Gly Ala Gln Met Ala Val 
225                 230                 235                 240 


Trp Ile Asn Val Gly Tyr Leu Phe Pro Leu Thr Tyr Leu Phe Val Asp 
                245                 250                 255     


Phe Ala Lys Arg Thr Tyr Ser Lys Arg Ser Ala Ile Ala Ala Gln Lys 
            260                 265                 270         


Lys Ala Gln 
        275 


<210>  62
<211>  1209
<212>  DNA
<213>  Fusarium monoliforme


<220>
<221>  CDS
<222>  (1)..(1209)
<223>  delta-15 desaturase

<300>
<302>  DELTA-15 DESATURASES SUITABLE FOR ALTERING LEVELS OF 
       POLYUNSATURATED FATTY ACIDS IN OLEAGINOUS PLANTS AND YEAST
<310>  WO 2005/047480
<311>  2004-11-10
<312>  2005-05-26
<313>  (1)..(1209)

<300>
<302>  DELTA-15 DESATURASES SUITABLE FOR ALTERING LEVELS OF 
       POLYUNSATURATED FATTY ACIDS IN OLEAGINOUS PLANTS AND YEAST
<310>  US 2005-0132442-A1
<311>  2004-11-10
<312>  2005-06-16
<313>  (1)..(1209)

<400>  62
atg gcg act cga cag cga act gcc acc act gtt gtg gtc gag gac ctt       48
Met Ala Thr Arg Gln Arg Thr Ala Thr Thr Val Val Val Glu Asp Leu         
1               5                   10                  15              

ccc aag gtc act ctt gag gcc aag tct gaa cct gtg ttc ccc gat atc       96
Pro Lys Val Thr Leu Glu Ala Lys Ser Glu Pro Val Phe Pro Asp Ile         
            20                  25                  30                  

aag acc atc aag gat gcc att ccc gcg cac tgc ttc cag ccc tcg ctc      144
Lys Thr Ile Lys Asp Ala Ile Pro Ala His Cys Phe Gln Pro Ser Leu         
        35                  40                  45                      

gtc acc tca ttc tac tac gtc ttc cgc gat ttt gcc atg gtc tct gcc      192
Val Thr Ser Phe Tyr Tyr Val Phe Arg Asp Phe Ala Met Val Ser Ala         
    50                  55                  60                          

ctc gtc tgg gct gct ctc acc tac atc ccc agc atc ccc gac cag acc      240
Leu Val Trp Ala Ala Leu Thr Tyr Ile Pro Ser Ile Pro Asp Gln Thr         
65                  70                  75                  80          

ctc cgc gtc gca gct tgg atg gtc tac ggc ttc gtc cag ggt ctg ttc      288
Leu Arg Val Ala Ala Trp Met Val Tyr Gly Phe Val Gln Gly Leu Phe         
                85                  90                  95              

tgc acc ggt gtc tgg att ctc ggc cat gag tgc ggc cac ggt gct ttc      336
Cys Thr Gly Val Trp Ile Leu Gly His Glu Cys Gly His Gly Ala Phe         
            100                 105                 110                 

tct ctc cac gga aag gtc aac aat gtg acc ggc tgg ttc ctc cac tcg      384
Ser Leu His Gly Lys Val Asn Asn Val Thr Gly Trp Phe Leu His Ser         
        115                 120                 125                     

ttc ctc ctc gtc ccc tac ttc agc tgg aag tac tct cac cac cgc cac      432
Phe Leu Leu Val Pro Tyr Phe Ser Trp Lys Tyr Ser His His Arg His         
    130                 135                 140                         

cac cgc ttc acc ggc cac atg gat ctc gac atg gct ttc gtc ccc aag      480
His Arg Phe Thr Gly His Met Asp Leu Asp Met Ala Phe Val Pro Lys         
145                 150                 155                 160         

act gag ccc aag ccc tcc aag tcg ctc atg att gct ggc att gac gtc      528
Thr Glu Pro Lys Pro Ser Lys Ser Leu Met Ile Ala Gly Ile Asp Val         
                165                 170                 175             

gcc gag ctt gtt gag gac acc ccc gct gct cag atg gtc aag ctc atc      576
Ala Glu Leu Val Glu Asp Thr Pro Ala Ala Gln Met Val Lys Leu Ile         
            180                 185                 190                 

ttc cac cag ctt ttc gga tgg cag gcg tac ctc ttc ttc aac gct agc      624
Phe His Gln Leu Phe Gly Trp Gln Ala Tyr Leu Phe Phe Asn Ala Ser         
        195                 200                 205                     

tct ggc aag ggc agc aag cag tgg gag ccc aag act ggc ctc tcc aag      672
Ser Gly Lys Gly Ser Lys Gln Trp Glu Pro Lys Thr Gly Leu Ser Lys         
    210                 215                 220                         

tgg ttc cga gtc agt cac ttc gag cct acc agc gct gtc ttc cgc ccc      720
Trp Phe Arg Val Ser His Phe Glu Pro Thr Ser Ala Val Phe Arg Pro         
225                 230                 235                 240         

aac gag gcc atc ttc atc ctc atc tcc gat atc ggt ctt gct cta atg      768
Asn Glu Ala Ile Phe Ile Leu Ile Ser Asp Ile Gly Leu Ala Leu Met         
                245                 250                 255             

gga act gct ctg tac ttt gct tcc aag caa gtt ggt gtt tcg acc att      816
Gly Thr Ala Leu Tyr Phe Ala Ser Lys Gln Val Gly Val Ser Thr Ile         
            260                 265                 270                 

ctc ttc ctc tac ctt gtt ccc tac ctg tgg gtt cac cac tgg ctc gtt      864
Leu Phe Leu Tyr Leu Val Pro Tyr Leu Trp Val His His Trp Leu Val         
        275                 280                 285                     

gcc att acc tac ctc cac cac cac cac acc gag ctc cct cac tac acc      912
Ala Ile Thr Tyr Leu His His His His Thr Glu Leu Pro His Tyr Thr         
    290                 295                 300                         

gct gag ggc tgg acc tac gtc aag gga gct ctc gcc act gtc gac cgt      960
Ala Glu Gly Trp Thr Tyr Val Lys Gly Ala Leu Ala Thr Val Asp Arg         
305                 310                 315                 320         

gag ttt ggc ttc atc gga aag cac ctc ttc cac ggt atc att gag aag     1008
Glu Phe Gly Phe Ile Gly Lys His Leu Phe His Gly Ile Ile Glu Lys         
                325                 330                 335             

cac gtt gtt cac cat ctc ttc cct aag atc ccc ttc tac aag gct gac     1056
His Val Val His His Leu Phe Pro Lys Ile Pro Phe Tyr Lys Ala Asp         
            340                 345                 350                 

gag gcc acc gag gcc atc aag ccc gtc att ggc gac cac tac tgc cac     1104
Glu Ala Thr Glu Ala Ile Lys Pro Val Ile Gly Asp His Tyr Cys His         
        355                 360                 365                     

gac gac cga agc ttc ctg ggc cag ctg tgg acc atc ttc ggc acg ctc     1152
Asp Asp Arg Ser Phe Leu Gly Gln Leu Trp Thr Ile Phe Gly Thr Leu         
    370                 375                 380                         

aag tac gtc gag cac gac cct gcc cga ccc ggt gcc atg cga tgg aac     1200
Lys Tyr Val Glu His Asp Pro Ala Arg Pro Gly Ala Met Arg Trp Asn         
385                 390                 395                 400         

aag gac tag                                                         1209
Lys Asp                                                                 
                                                                        


<210>  63
<211>  402
<212>  PRT
<213>  Fusarium monoliforme

<400>  63

Met Ala Thr Arg Gln Arg Thr Ala Thr Thr Val Val Val Glu Asp Leu 
1               5                   10                  15      


Pro Lys Val Thr Leu Glu Ala Lys Ser Glu Pro Val Phe Pro Asp Ile 
            20                  25                  30          


Lys Thr Ile Lys Asp Ala Ile Pro Ala His Cys Phe Gln Pro Ser Leu 
        35                  40                  45              


Val Thr Ser Phe Tyr Tyr Val Phe Arg Asp Phe Ala Met Val Ser Ala 
    50                  55                  60                  


Leu Val Trp Ala Ala Leu Thr Tyr Ile Pro Ser Ile Pro Asp Gln Thr 
65                  70                  75                  80  


Leu Arg Val Ala Ala Trp Met Val Tyr Gly Phe Val Gln Gly Leu Phe 
                85                  90                  95      


Cys Thr Gly Val Trp Ile Leu Gly His Glu Cys Gly His Gly Ala Phe 
            100                 105                 110         


Ser Leu His Gly Lys Val Asn Asn Val Thr Gly Trp Phe Leu His Ser 
        115                 120                 125             


Phe Leu Leu Val Pro Tyr Phe Ser Trp Lys Tyr Ser His His Arg His 
    130                 135                 140                 


His Arg Phe Thr Gly His Met Asp Leu Asp Met Ala Phe Val Pro Lys 
145                 150                 155                 160 


Thr Glu Pro Lys Pro Ser Lys Ser Leu Met Ile Ala Gly Ile Asp Val 
                165                 170                 175     


Ala Glu Leu Val Glu Asp Thr Pro Ala Ala Gln Met Val Lys Leu Ile 
            180                 185                 190         


Phe His Gln Leu Phe Gly Trp Gln Ala Tyr Leu Phe Phe Asn Ala Ser 
        195                 200                 205             


Ser Gly Lys Gly Ser Lys Gln Trp Glu Pro Lys Thr Gly Leu Ser Lys 
    210                 215                 220                 


Trp Phe Arg Val Ser His Phe Glu Pro Thr Ser Ala Val Phe Arg Pro 
225                 230                 235                 240 


Asn Glu Ala Ile Phe Ile Leu Ile Ser Asp Ile Gly Leu Ala Leu Met 
                245                 250                 255     


Gly Thr Ala Leu Tyr Phe Ala Ser Lys Gln Val Gly Val Ser Thr Ile 
            260                 265                 270         


Leu Phe Leu Tyr Leu Val Pro Tyr Leu Trp Val His His Trp Leu Val 
        275                 280                 285             


Ala Ile Thr Tyr Leu His His His His Thr Glu Leu Pro His Tyr Thr 
    290                 295                 300                 


Ala Glu Gly Trp Thr Tyr Val Lys Gly Ala Leu Ala Thr Val Asp Arg 
305                 310                 315                 320 


Glu Phe Gly Phe Ile Gly Lys His Leu Phe His Gly Ile Ile Glu Lys 
                325                 330                 335     


His Val Val His His Leu Phe Pro Lys Ile Pro Phe Tyr Lys Ala Asp 
            340                 345                 350         


Glu Ala Thr Glu Ala Ile Lys Pro Val Ile Gly Asp His Tyr Cys His 
        355                 360                 365             


Asp Asp Arg Ser Phe Leu Gly Gln Leu Trp Thr Ile Phe Gly Thr Leu 
    370                 375                 380                 


Lys Tyr Val Glu His Asp Pro Ala Arg Pro Gly Ala Met Arg Trp Asn 
385                 390                 395                 400 


Lys Asp 
        


<210>  64
<211>  1209
<212>  DNA
<213>  Fusarium moniliforme


<220>
<221>  CDS
<222>  (1)..(1209)
<223>  synthetic delta-15 desaturase (codon-optimized for Yarrowia 
       lipolytica)

<300>
<302>  DELTA-15 DESATURASES SUITABLE FOR ALTERING LEVELS OF 
       POLYUNSATURATED FATTY ACIDS IN OLEAGINOUS PLANTS AND YEAST
<310>  WO 2005/047480
<311>  2004-11-10
<312>  2005-05-26
<313>  (1)..(1209)

<300>
<302>  DELTA-15 DESATURASES SUITABLE FOR ALTERING LEVELS OF 
       POLYUNSATURATED FATTY ACIDS IN OLEAGINOUS PLANTS AND YEAST
<310>  US 2005-0132442-A1
<311>  2004-11-10
<312>  2005-06-16
<313>  (1)..(1209)

<400>  64
atg gcc acc cga cag cga act gct acc act gtc gtg gtc gag gac ctg       48
Met Ala Thr Arg Gln Arg Thr Ala Thr Thr Val Val Val Glu Asp Leu         
1               5                   10                  15              

ccc aag gtt acc ctc gag gcc aag tcc gaa cct gtc ttt ccc gac atc       96
Pro Lys Val Thr Leu Glu Ala Lys Ser Glu Pro Val Phe Pro Asp Ile         
            20                  25                  30                  

aag acc atc aag gat gcc att cct gct cac tgc ttt cag ccc tct ctg      144
Lys Thr Ile Lys Asp Ala Ile Pro Ala His Cys Phe Gln Pro Ser Leu         
        35                  40                  45                      

gtc acc tcc ttc tac tat gtg ttc cga gac ttt gct atg gtt tct gcc      192
Val Thr Ser Phe Tyr Tyr Val Phe Arg Asp Phe Ala Met Val Ser Ala         
    50                  55                  60                          

ctc gtc tgg gct gcc ctt acc tac att ccc tcg atc cct gat cag act      240
Leu Val Trp Ala Ala Leu Thr Tyr Ile Pro Ser Ile Pro Asp Gln Thr         
65                  70                  75                  80          

ctg cga gtg gca gct tgg atg gtc tac ggc ttc gtt cag gga ctc ttc      288
Leu Arg Val Ala Ala Trp Met Val Tyr Gly Phe Val Gln Gly Leu Phe         
                85                  90                  95              

tgt acc ggt gtc tgg att ctc gga cac gag tgc ggt cat gga gcc ttc      336
Cys Thr Gly Val Trp Ile Leu Gly His Glu Cys Gly His Gly Ala Phe         
            100                 105                 110                 

tct ctg cac ggc aag gtc aac aat gtc acc gga tgg ttt ctt cat tcc      384
Ser Leu His Gly Lys Val Asn Asn Val Thr Gly Trp Phe Leu His Ser         
        115                 120                 125                     

ttc ctg ctc gtt ccc tac ttc agc tgg aag tac tct cat cac cga cat      432
Phe Leu Leu Val Pro Tyr Phe Ser Trp Lys Tyr Ser His His Arg His         
    130                 135                 140                         

cac cga ttc aca ggt cac atg gat ctg gac atg gct ttc gtt ccc aag      480
His Arg Phe Thr Gly His Met Asp Leu Asp Met Ala Phe Val Pro Lys         
145                 150                 155                 160         

acc gag ccc aaa ccc tcc aag tct ctc atg att gct ggc att gac gtt      528
Thr Glu Pro Lys Pro Ser Lys Ser Leu Met Ile Ala Gly Ile Asp Val         
                165                 170                 175             

gcc gaa ctt gtc gag gac act cct gct gcc cag atg gtc aag ctc atc      576
Ala Glu Leu Val Glu Asp Thr Pro Ala Ala Gln Met Val Lys Leu Ile         
            180                 185                 190                 

ttc cat cag ctg ttc gga tgg cag gcg tac ctc ttc ttc aac gcc agc      624
Phe His Gln Leu Phe Gly Trp Gln Ala Tyr Leu Phe Phe Asn Ala Ser         
        195                 200                 205                     

tct ggc aag ggt tcc aag cag tgg gag ccc aag act gga ctc tcg aag      672
Ser Gly Lys Gly Ser Lys Gln Trp Glu Pro Lys Thr Gly Leu Ser Lys         
    210                 215                 220                         

tgg ttt cga gtg tct cac ttc gag cct acc agc gct gtc ttc aga ccc      720
Trp Phe Arg Val Ser His Phe Glu Pro Thr Ser Ala Val Phe Arg Pro         
225                 230                 235                 240         

aac gag gcc atc ttc att ctc atc tcg gac atc ggt ctt gct ctc atg      768
Asn Glu Ala Ile Phe Ile Leu Ile Ser Asp Ile Gly Leu Ala Leu Met         
                245                 250                 255             

ggc act gca ctg tac ttt gct tcc aag caa gtc gga gtt tct acc att      816
Gly Thr Ala Leu Tyr Phe Ala Ser Lys Gln Val Gly Val Ser Thr Ile         
            260                 265                 270                 

ctg ttc ctc tac ctt gtt ccc tac ctg tgg gtc cat cac tgg ctc gtg      864
Leu Phe Leu Tyr Leu Val Pro Tyr Leu Trp Val His His Trp Leu Val         
        275                 280                 285                     

gcc att act tac ctt cac cat cac cat acc gaa ctg cct cac tac acc      912
Ala Ile Thr Tyr Leu His His His His Thr Glu Leu Pro His Tyr Thr         
    290                 295                 300                         

gct gag ggc tgg acc tac gtc aag ggt gca ctc gcc act gtg gat cga      960
Ala Glu Gly Trp Thr Tyr Val Lys Gly Ala Leu Ala Thr Val Asp Arg         
305                 310                 315                 320         

gag ttt gga ttc atc ggc aag cat ctc ttt cac ggt atc att gag aag     1008
Glu Phe Gly Phe Ile Gly Lys His Leu Phe His Gly Ile Ile Glu Lys         
                325                 330                 335             

cac gtt gtg cat cac ttg ttt ccc aag att ccc ttc tac aag gct gac     1056
His Val Val His His Leu Phe Pro Lys Ile Pro Phe Tyr Lys Ala Asp         
            340                 345                 350                 

gaa gcc acc gag gcc atc aag cct gtc att ggc gac cac tac tgt cac     1104
Glu Ala Thr Glu Ala Ile Lys Pro Val Ile Gly Asp His Tyr Cys His         
        355                 360                 365                     

gac gat cgg tcc ttc ctg ggt cag ctg tgg acc atc ttc gga act ctc     1152
Asp Asp Arg Ser Phe Leu Gly Gln Leu Trp Thr Ile Phe Gly Thr Leu         
    370                 375                 380                         

aag tac gtg gag cac gat cct gcc cga ccc ggt gcc atg cga tgg aac     1200
Lys Tyr Val Glu His Asp Pro Ala Arg Pro Gly Ala Met Arg Trp Asn         
385                 390                 395                 400         

aag gac taa                                                         1209
Lys Asp                                                                 
                                                                        


<210>  65
<211>  402
<212>  PRT
<213>  Fusarium moniliforme

<400>  65

Met Ala Thr Arg Gln Arg Thr Ala Thr Thr Val Val Val Glu Asp Leu 
1               5                   10                  15      


Pro Lys Val Thr Leu Glu Ala Lys Ser Glu Pro Val Phe Pro Asp Ile 
            20                  25                  30          


Lys Thr Ile Lys Asp Ala Ile Pro Ala His Cys Phe Gln Pro Ser Leu 
        35                  40                  45              


Val Thr Ser Phe Tyr Tyr Val Phe Arg Asp Phe Ala Met Val Ser Ala 
    50                  55                  60                  


Leu Val Trp Ala Ala Leu Thr Tyr Ile Pro Ser Ile Pro Asp Gln Thr 
65                  70                  75                  80  


Leu Arg Val Ala Ala Trp Met Val Tyr Gly Phe Val Gln Gly Leu Phe 
                85                  90                  95      


Cys Thr Gly Val Trp Ile Leu Gly His Glu Cys Gly His Gly Ala Phe 
            100                 105                 110         


Ser Leu His Gly Lys Val Asn Asn Val Thr Gly Trp Phe Leu His Ser 
        115                 120                 125             


Phe Leu Leu Val Pro Tyr Phe Ser Trp Lys Tyr Ser His His Arg His 
    130                 135                 140                 


His Arg Phe Thr Gly His Met Asp Leu Asp Met Ala Phe Val Pro Lys 
145                 150                 155                 160 


Thr Glu Pro Lys Pro Ser Lys Ser Leu Met Ile Ala Gly Ile Asp Val 
                165                 170                 175     


Ala Glu Leu Val Glu Asp Thr Pro Ala Ala Gln Met Val Lys Leu Ile 
            180                 185                 190         


Phe His Gln Leu Phe Gly Trp Gln Ala Tyr Leu Phe Phe Asn Ala Ser 
        195                 200                 205             


Ser Gly Lys Gly Ser Lys Gln Trp Glu Pro Lys Thr Gly Leu Ser Lys 
    210                 215                 220                 


Trp Phe Arg Val Ser His Phe Glu Pro Thr Ser Ala Val Phe Arg Pro 
225                 230                 235                 240 


Asn Glu Ala Ile Phe Ile Leu Ile Ser Asp Ile Gly Leu Ala Leu Met 
                245                 250                 255     


Gly Thr Ala Leu Tyr Phe Ala Ser Lys Gln Val Gly Val Ser Thr Ile 
            260                 265                 270         


Leu Phe Leu Tyr Leu Val Pro Tyr Leu Trp Val His His Trp Leu Val 
        275                 280                 285             


Ala Ile Thr Tyr Leu His His His His Thr Glu Leu Pro His Tyr Thr 
    290                 295                 300                 


Ala Glu Gly Trp Thr Tyr Val Lys Gly Ala Leu Ala Thr Val Asp Arg 
305                 310                 315                 320 


Glu Phe Gly Phe Ile Gly Lys His Leu Phe His Gly Ile Ile Glu Lys 
                325                 330                 335     


His Val Val His His Leu Phe Pro Lys Ile Pro Phe Tyr Lys Ala Asp 
            340                 345                 350         


Glu Ala Thr Glu Ala Ile Lys Pro Val Ile Gly Asp His Tyr Cys His 
        355                 360                 365             


Asp Asp Arg Ser Phe Leu Gly Gln Leu Trp Thr Ile Phe Gly Thr Leu 
    370                 375                 380                 


Lys Tyr Val Glu His Asp Pro Ala Arg Pro Gly Ala Met Arg Trp Asn 
385                 390                 395                 400 


Lys Asp 
        


<210>  66
<211>  1449
<212>  DNA
<213>  Yarrowia lipolytica


<220>
<221>  CDS
<222>  (1)..(1449)
<223>  delta-9 desaturase

<400>  66
atg gtg aaa aac gtg gac caa gtg gat ctc tcg cag gtc gac acc att       48
Met Val Lys Asn Val Asp Gln Val Asp Leu Ser Gln Val Asp Thr Ile         
1               5                   10                  15              

gcc tcc ggc cga gat gtc aac tac aag gtc aag tac acc tcc ggc gtt       96
Ala Ser Gly Arg Asp Val Asn Tyr Lys Val Lys Tyr Thr Ser Gly Val         
            20                  25                  30                  

aag atg agc cag ggc gcc tac gac gac aag ggc cgc cac att tcc gag      144
Lys Met Ser Gln Gly Ala Tyr Asp Asp Lys Gly Arg His Ile Ser Glu         
        35                  40                  45                      

cag ccc ttc acc tgg gcc aac tgg cac cag cac atc aac tgg ctc aac      192
Gln Pro Phe Thr Trp Ala Asn Trp His Gln His Ile Asn Trp Leu Asn         
    50                  55                  60                          

ttc att ctg gtg att gcg ctg cct ctg tcg tcc ttt gct gcc gct ccc      240
Phe Ile Leu Val Ile Ala Leu Pro Leu Ser Ser Phe Ala Ala Ala Pro         
65                  70                  75                  80          

ttc gtc tcc ttc aac tgg aag acc gcc gcg ttt gct gtc ggc tat tac      288
Phe Val Ser Phe Asn Trp Lys Thr Ala Ala Phe Ala Val Gly Tyr Tyr         
                85                  90                  95              

atg tgc acc ggt ctc ggt atc acc gcc ggc tac cac cga atg tgg gcc      336
Met Cys Thr Gly Leu Gly Ile Thr Ala Gly Tyr His Arg Met Trp Ala         
            100                 105                 110                 

cat cga gcc tac aag gcc gct ctg ccc gtt cga atc atc ctt gct ctg      384
His Arg Ala Tyr Lys Ala Ala Leu Pro Val Arg Ile Ile Leu Ala Leu         
        115                 120                 125                     

ttt gga gga gga gct gtc gag ggc tcc atc cga tgg tgg gcc tcg tct      432
Phe Gly Gly Gly Ala Val Glu Gly Ser Ile Arg Trp Trp Ala Ser Ser         
    130                 135                 140                         

cac cga gtc cac cac cga tgg acc gac tcc aac aag gac cct tac gac      480
His Arg Val His His Arg Trp Thr Asp Ser Asn Lys Asp Pro Tyr Asp         
145                 150                 155                 160         

gcc cga aag gga ttc tgg ttc tcc cac ttt ggc tgg atg ctg ctt gtg      528
Ala Arg Lys Gly Phe Trp Phe Ser His Phe Gly Trp Met Leu Leu Val         
                165                 170                 175             

ccc aac ccc aag aac aag ggc cga act gac att tct gac ctc aac aac      576
Pro Asn Pro Lys Asn Lys Gly Arg Thr Asp Ile Ser Asp Leu Asn Asn         
            180                 185                 190                 

gac tgg gtt gtc cga ctc cag cac aag tac tac gtt tac gtt ctc gtc      624
Asp Trp Val Val Arg Leu Gln His Lys Tyr Tyr Val Tyr Val Leu Val         
        195                 200                 205                     

ttc atg gcc att gtt ctg ccc acc ctc gtc tgt ggc ttt ggc tgg ggc      672
Phe Met Ala Ile Val Leu Pro Thr Leu Val Cys Gly Phe Gly Trp Gly         
    210                 215                 220                         

gac tgg aag gga ggt ctt gtc tac gcc ggt atc atg cga tac acc ttt      720
Asp Trp Lys Gly Gly Leu Val Tyr Ala Gly Ile Met Arg Tyr Thr Phe         
225                 230                 235                 240         

gtg cag cag gtg act ttc tgt gtc aac tcc ctt gcc cac tgg att gga      768
Val Gln Gln Val Thr Phe Cys Val Asn Ser Leu Ala His Trp Ile Gly         
                245                 250                 255             

gag cag ccc ttc gac gac cga cga act ccc cga gac cac gct ctt acc      816
Glu Gln Pro Phe Asp Asp Arg Arg Thr Pro Arg Asp His Ala Leu Thr         
            260                 265                 270                 

gcc ctg gtc acc ttt gga gag ggc tac cac aac ttc cac cac gag ttc      864
Ala Leu Val Thr Phe Gly Glu Gly Tyr His Asn Phe His His Glu Phe         
        275                 280                 285                     

ccc tcg gac tac cga aac gcc ctc atc tgg tac cag tac gac ccc acc      912
Pro Ser Asp Tyr Arg Asn Ala Leu Ile Trp Tyr Gln Tyr Asp Pro Thr         
    290                 295                 300                         

aag tgg ctc atc tgg acc ctc aag cag gtt ggt ctc gcc tgg gac ctc      960
Lys Trp Leu Ile Trp Thr Leu Lys Gln Val Gly Leu Ala Trp Asp Leu         
305                 310                 315                 320         

cag acc ttc tcc cag aac gcc atc gag cag ggt ctc gtg cag cag cga     1008
Gln Thr Phe Ser Gln Asn Ala Ile Glu Gln Gly Leu Val Gln Gln Arg         
                325                 330                 335             

cag aag aag ctg gac aag tgg cga aac aac ctc aac tgg ggt atc ccc     1056
Gln Lys Lys Leu Asp Lys Trp Arg Asn Asn Leu Asn Trp Gly Ile Pro         
            340                 345                 350                 

att gag cag ctg cct gtc att gag ttt gag gag ttc caa gag cag gcc     1104
Ile Glu Gln Leu Pro Val Ile Glu Phe Glu Glu Phe Gln Glu Gln Ala         
        355                 360                 365                     

aag acc cga gat ctg gtt ctc att tct ggc att gtc cac gac gtg tct     1152
Lys Thr Arg Asp Leu Val Leu Ile Ser Gly Ile Val His Asp Val Ser         
    370                 375                 380                         

gcc ttt gtc gag cac cac cct ggt gga aag gcc ctc att atg agc gcc     1200
Ala Phe Val Glu His His Pro Gly Gly Lys Ala Leu Ile Met Ser Ala         
385                 390                 395                 400         

gtc ggc aag gac ggt acc gct gtc ttc aac gga ggt gtc tac cga cac     1248
Val Gly Lys Asp Gly Thr Ala Val Phe Asn Gly Gly Val Tyr Arg His         
                405                 410                 415             

tcc aac gct ggc cac aac ctg ctt gcc acc atg cga gtt tcg gtc att     1296
Ser Asn Ala Gly His Asn Leu Leu Ala Thr Met Arg Val Ser Val Ile         
            420                 425                 430                 

cga ggc ggc atg gag gtt gag gtg tgg aag act gcc cag aac gaa aag     1344
Arg Gly Gly Met Glu Val Glu Val Trp Lys Thr Ala Gln Asn Glu Lys         
        435                 440                 445                     

aag gac cag aac att gtc tcc gat gag agt gga aac cga atc cac cga     1392
Lys Asp Gln Asn Ile Val Ser Asp Glu Ser Gly Asn Arg Ile His Arg         
    450                 455                 460                         

gct ggt ctc cag gcc acc cgg gtc gag aac ccc ggt atg tct ggc atg     1440
Ala Gly Leu Gln Ala Thr Arg Val Glu Asn Pro Gly Met Ser Gly Met         
465                 470                 475                 480         

gct gct tag                                                         1449
Ala Ala                                                                 
                                                                        


<210>  67
<211>  482
<212>  PRT
<213>  Yarrowia lipolytica

<400>  67

Met Val Lys Asn Val Asp Gln Val Asp Leu Ser Gln Val Asp Thr Ile 
1               5                   10                  15      


Ala Ser Gly Arg Asp Val Asn Tyr Lys Val Lys Tyr Thr Ser Gly Val 
            20                  25                  30          


Lys Met Ser Gln Gly Ala Tyr Asp Asp Lys Gly Arg His Ile Ser Glu 
        35                  40                  45              


Gln Pro Phe Thr Trp Ala Asn Trp His Gln His Ile Asn Trp Leu Asn 
    50                  55                  60                  


Phe Ile Leu Val Ile Ala Leu Pro Leu Ser Ser Phe Ala Ala Ala Pro 
65                  70                  75                  80  


Phe Val Ser Phe Asn Trp Lys Thr Ala Ala Phe Ala Val Gly Tyr Tyr 
                85                  90                  95      


Met Cys Thr Gly Leu Gly Ile Thr Ala Gly Tyr His Arg Met Trp Ala 
            100                 105                 110         


His Arg Ala Tyr Lys Ala Ala Leu Pro Val Arg Ile Ile Leu Ala Leu 
        115                 120                 125             


Phe Gly Gly Gly Ala Val Glu Gly Ser Ile Arg Trp Trp Ala Ser Ser 
    130                 135                 140                 


His Arg Val His His Arg Trp Thr Asp Ser Asn Lys Asp Pro Tyr Asp 
145                 150                 155                 160 


Ala Arg Lys Gly Phe Trp Phe Ser His Phe Gly Trp Met Leu Leu Val 
                165                 170                 175     


Pro Asn Pro Lys Asn Lys Gly Arg Thr Asp Ile Ser Asp Leu Asn Asn 
            180                 185                 190         


Asp Trp Val Val Arg Leu Gln His Lys Tyr Tyr Val Tyr Val Leu Val 
        195                 200                 205             


Phe Met Ala Ile Val Leu Pro Thr Leu Val Cys Gly Phe Gly Trp Gly 
    210                 215                 220                 


Asp Trp Lys Gly Gly Leu Val Tyr Ala Gly Ile Met Arg Tyr Thr Phe 
225                 230                 235                 240 


Val Gln Gln Val Thr Phe Cys Val Asn Ser Leu Ala His Trp Ile Gly 
                245                 250                 255     


Glu Gln Pro Phe Asp Asp Arg Arg Thr Pro Arg Asp His Ala Leu Thr 
            260                 265                 270         


Ala Leu Val Thr Phe Gly Glu Gly Tyr His Asn Phe His His Glu Phe 
        275                 280                 285             


Pro Ser Asp Tyr Arg Asn Ala Leu Ile Trp Tyr Gln Tyr Asp Pro Thr 
    290                 295                 300                 


Lys Trp Leu Ile Trp Thr Leu Lys Gln Val Gly Leu Ala Trp Asp Leu 
305                 310                 315                 320 


Gln Thr Phe Ser Gln Asn Ala Ile Glu Gln Gly Leu Val Gln Gln Arg 
                325                 330                 335     


Gln Lys Lys Leu Asp Lys Trp Arg Asn Asn Leu Asn Trp Gly Ile Pro 
            340                 345                 350         


Ile Glu Gln Leu Pro Val Ile Glu Phe Glu Glu Phe Gln Glu Gln Ala 
        355                 360                 365             


Lys Thr Arg Asp Leu Val Leu Ile Ser Gly Ile Val His Asp Val Ser 
    370                 375                 380                 


Ala Phe Val Glu His His Pro Gly Gly Lys Ala Leu Ile Met Ser Ala 
385                 390                 395                 400 


Val Gly Lys Asp Gly Thr Ala Val Phe Asn Gly Gly Val Tyr Arg His 
                405                 410                 415     


Ser Asn Ala Gly His Asn Leu Leu Ala Thr Met Arg Val Ser Val Ile 
            420                 425                 430         


Arg Gly Gly Met Glu Val Glu Val Trp Lys Thr Ala Gln Asn Glu Lys 
        435                 440                 445             


Lys Asp Gln Asn Ile Val Ser Asp Glu Ser Gly Asn Arg Ile His Arg 
    450                 455                 460                 


Ala Gly Leu Gln Ala Thr Arg Val Glu Asn Pro Gly Met Ser Gly Met 
465                 470                 475                 480 


Ala Ala 
        


<210>  68
<211>  1185
<212>  DNA
<213>  Yarrowia lipolytica


<220>
<221>  CDS
<222>  (1)..(1185)
<223>  diacylglycerol cholinephosphotransferase (YlCPT1)

<300>
<302>  HIGH EICOSAPENTAENOIC ACID PRODUCING STRAINS OF
<310>  WO 2006/052870
<311>  2005-11-03
<312>  2006-05-18
<313>  (1)..(1185)

<300>
<302>  HIGH EICOSAPENTAENOIC ACID PRODUCING STRAINS OF
<310>  US 2006-0115881-A1
<311>  2005-11-02
<312>  2006-06-01
<313>  (1)..(1185)

<400>  68
atg ggc gta ttc att aaa cag gag cag ctt ccg gct ctc aag aag tac       48
Met Gly Val Phe Ile Lys Gln Glu Gln Leu Pro Ala Leu Lys Lys Tyr         
1               5                   10                  15              

aag tac tcc gcc gag gat cac tcg ttc atc tcc aac aac att ctg cgc       96
Lys Tyr Ser Ala Glu Asp His Ser Phe Ile Ser Asn Asn Ile Leu Arg         
            20                  25                  30                  

ccc ttc tgg cga cag ttt gtc aaa atc ttc cct ctg tgg atg gcc ccc      144
Pro Phe Trp Arg Gln Phe Val Lys Ile Phe Pro Leu Trp Met Ala Pro         
        35                  40                  45                      

aac atg gtg act ctg ttg ggc ttc ttc ttt gtc att gtg aac ttc atc      192
Asn Met Val Thr Leu Leu Gly Phe Phe Phe Val Ile Val Asn Phe Ile         
    50                  55                  60                          

acc atg ctc att gtt gat ccc acc cac gac cgc gag cct ccc aga tgg      240
Thr Met Leu Ile Val Asp Pro Thr His Asp Arg Glu Pro Pro Arg Trp         
65                  70                  75                  80          

gtc tac ctc acc tac gct ctg ggt ctg ttc ctt tac cag aca ttt gat      288
Val Tyr Leu Thr Tyr Ala Leu Gly Leu Phe Leu Tyr Gln Thr Phe Asp         
                85                  90                  95              

gcc tgt gac gga tcc cat gcc cga cga act ggc cag agt gga ccc ctt      336
Ala Cys Asp Gly Ser His Ala Arg Arg Thr Gly Gln Ser Gly Pro Leu         
            100                 105                 110                 

gga gag ctg ttt gac cac tgt gtc gac gcc atg aat acc tct ctg att      384
Gly Glu Leu Phe Asp His Cys Val Asp Ala Met Asn Thr Ser Leu Ile         
        115                 120                 125                     

ctc acg gtg gtg gtg tcc acc acc cat atg gga tat aac atg aag ctg      432
Leu Thr Val Val Val Ser Thr Thr His Met Gly Tyr Asn Met Lys Leu         
    130                 135                 140                         

ctg att gtg cag att gcc gct ctc gga aac ttc tac ctg tcg acc tgg      480
Leu Ile Val Gln Ile Ala Ala Leu Gly Asn Phe Tyr Leu Ser Thr Trp         
145                 150                 155                 160         

gag acc tac cat acc gga act ctg tac ctt tct ggc ttc tct ggt cct      528
Glu Thr Tyr His Thr Gly Thr Leu Tyr Leu Ser Gly Phe Ser Gly Pro         
                165                 170                 175             

gtt gaa ggt atc ttg att ctg gtg gct ctt ttc gtc ctc acc ttc ttc      576
Val Glu Gly Ile Leu Ile Leu Val Ala Leu Phe Val Leu Thr Phe Phe         
            180                 185                 190                 

act ggt ccc aac gtg tac gct ctg acc gtc tac gag gct ctt ccc gaa      624
Thr Gly Pro Asn Val Tyr Ala Leu Thr Val Tyr Glu Ala Leu Pro Glu         
        195                 200                 205                     

tcc atc act tcg ctg ctg cct gcc agc ttc ctg gac gtc acc atc acc      672
Ser Ile Thr Ser Leu Leu Pro Ala Ser Phe Leu Asp Val Thr Ile Thr         
    210                 215                 220                         

cag atc tac att gga ttc gga gtg ctg ggc atg gtg ttc aac atc tac      720
Gln Ile Tyr Ile Gly Phe Gly Val Leu Gly Met Val Phe Asn Ile Tyr         
225                 230                 235                 240         

ggc gcc tgc gga aac gtg atc aag tac tac aac aac aag ggc aag agc      768
Gly Ala Cys Gly Asn Val Ile Lys Tyr Tyr Asn Asn Lys Gly Lys Ser         
                245                 250                 255             

gct ctc ccc gcc att ctc gga atc gcc ccc ttt ggc atc ttc tac gtc      816
Ala Leu Pro Ala Ile Leu Gly Ile Ala Pro Phe Gly Ile Phe Tyr Val         
            260                 265                 270                 

ggc gtc ttt gcc tgg gcc cat gtt gct cct ctg ctt ctc tcc aag tac      864
Gly Val Phe Ala Trp Ala His Val Ala Pro Leu Leu Leu Ser Lys Tyr         
        275                 280                 285                     

gcc atc gtc tat ctg ttt gcc att ggg gct gcc ttt gcc atg caa gtc      912
Ala Ile Val Tyr Leu Phe Ala Ile Gly Ala Ala Phe Ala Met Gln Val         
    290                 295                 300                         

ggc cag atg att ctt gcc cat ctc gtg ctt gct ccc ttc ccc cac tgg      960
Gly Gln Met Ile Leu Ala His Leu Val Leu Ala Pro Phe Pro His Trp         
305                 310                 315                 320         

aac gtg ctg ctc ttc ttc ccc ttt gtg gga ctg gca gtg cac tac att     1008
Asn Val Leu Leu Phe Phe Pro Phe Val Gly Leu Ala Val His Tyr Ile         
                325                 330                 335             

gca ccc gtg ttt ggc tgg gac gcc gat atc gtg tcg gtt aac act ctc     1056
Ala Pro Val Phe Gly Trp Asp Ala Asp Ile Val Ser Val Asn Thr Leu         
            340                 345                 350                 

ttc acc tgt ttt ggc gcc acc ctc tcc att tac gcc ttc ttt gtg ctt     1104
Phe Thr Cys Phe Gly Ala Thr Leu Ser Ile Tyr Ala Phe Phe Val Leu         
        355                 360                 365                     

gag atc atc gac gag atc acc aac tac ctc gat atc tgg tgt ctg cga     1152
Glu Ile Ile Asp Glu Ile Thr Asn Tyr Leu Asp Ile Trp Cys Leu Arg         
    370                 375                 380                         

atc aag tac cct cag gag aag aag act gag taa                         1185
Ile Lys Tyr Pro Gln Glu Lys Lys Thr Glu                                 
385                 390                                                 


<210>  69
<211>  394
<212>  PRT
<213>  Yarrowia lipolytica

<400>  69

Met Gly Val Phe Ile Lys Gln Glu Gln Leu Pro Ala Leu Lys Lys Tyr 
1               5                   10                  15      


Lys Tyr Ser Ala Glu Asp His Ser Phe Ile Ser Asn Asn Ile Leu Arg 
            20                  25                  30          


Pro Phe Trp Arg Gln Phe Val Lys Ile Phe Pro Leu Trp Met Ala Pro 
        35                  40                  45              


Asn Met Val Thr Leu Leu Gly Phe Phe Phe Val Ile Val Asn Phe Ile 
    50                  55                  60                  


Thr Met Leu Ile Val Asp Pro Thr His Asp Arg Glu Pro Pro Arg Trp 
65                  70                  75                  80  


Val Tyr Leu Thr Tyr Ala Leu Gly Leu Phe Leu Tyr Gln Thr Phe Asp 
                85                  90                  95      


Ala Cys Asp Gly Ser His Ala Arg Arg Thr Gly Gln Ser Gly Pro Leu 
            100                 105                 110         


Gly Glu Leu Phe Asp His Cys Val Asp Ala Met Asn Thr Ser Leu Ile 
        115                 120                 125             


Leu Thr Val Val Val Ser Thr Thr His Met Gly Tyr Asn Met Lys Leu 
    130                 135                 140                 


Leu Ile Val Gln Ile Ala Ala Leu Gly Asn Phe Tyr Leu Ser Thr Trp 
145                 150                 155                 160 


Glu Thr Tyr His Thr Gly Thr Leu Tyr Leu Ser Gly Phe Ser Gly Pro 
                165                 170                 175     


Val Glu Gly Ile Leu Ile Leu Val Ala Leu Phe Val Leu Thr Phe Phe 
            180                 185                 190         


Thr Gly Pro Asn Val Tyr Ala Leu Thr Val Tyr Glu Ala Leu Pro Glu 
        195                 200                 205             


Ser Ile Thr Ser Leu Leu Pro Ala Ser Phe Leu Asp Val Thr Ile Thr 
    210                 215                 220                 


Gln Ile Tyr Ile Gly Phe Gly Val Leu Gly Met Val Phe Asn Ile Tyr 
225                 230                 235                 240 


Gly Ala Cys Gly Asn Val Ile Lys Tyr Tyr Asn Asn Lys Gly Lys Ser 
                245                 250                 255     


Ala Leu Pro Ala Ile Leu Gly Ile Ala Pro Phe Gly Ile Phe Tyr Val 
            260                 265                 270         


Gly Val Phe Ala Trp Ala His Val Ala Pro Leu Leu Leu Ser Lys Tyr 
        275                 280                 285             


Ala Ile Val Tyr Leu Phe Ala Ile Gly Ala Ala Phe Ala Met Gln Val 
    290                 295                 300                 


Gly Gln Met Ile Leu Ala His Leu Val Leu Ala Pro Phe Pro His Trp 
305                 310                 315                 320 


Asn Val Leu Leu Phe Phe Pro Phe Val Gly Leu Ala Val His Tyr Ile 
                325                 330                 335     


Ala Pro Val Phe Gly Trp Asp Ala Asp Ile Val Ser Val Asn Thr Leu 
            340                 345                 350         


Phe Thr Cys Phe Gly Ala Thr Leu Ser Ile Tyr Ala Phe Phe Val Leu 
        355                 360                 365             


Glu Ile Ile Asp Glu Ile Thr Asn Tyr Leu Asp Ile Trp Cys Leu Arg 
    370                 375                 380                 


Ile Lys Tyr Pro Gln Glu Lys Lys Thr Glu 
385                 390                 


<210>  70
<211>  4844
<212>  DNA
<213>  Yarrowia lipolytica (GenBank Accession No. AJ306421)


<220>
<221>  CDS
<222>  (1195)..(2049)
<223>  Ura3

<400>  70
acagtatcat taggaaaggg gtgacactgc actattggtt tgcttctgat gatacgtatg     60

aatacgatgg tatcttgata cagagtcttg catacattaa tttcgaaact agagcacagc    120

aacagctccc aaatgagcat tcccctgaag cccatcaaaa ctgcaccaca aatttgatcc    180

catattacca ttttaaagca attgttaaca agatactact gtagaaaact gattaaacat    240

tcgtaacttg cctgtggctc gacttacagc caaatgttcg cggcaaaatt tgaatatttt    300

gcacacttat tgtcccaaaa ccaaataatg ccaacaaaat ccgatatagt gtaggggcta    360

tcacatcacg ctctcatcaa gaatacttct tgagaaccgt ggagaccggg gttcgattcc    420

ccgtatcgga gtgtttattt tttgctcaac cataccctgg ggtgtgttct gtggagcatt    480

ctcacttttg gtaaacgaca ttgcttcaag tgcagcggaa tcaaaaagta taaagtgggc    540

agcgagtata cctgtacaga ctgtaggcga taactcaatc caattacccc ccacaacatg    600

actggccaaa ctgatctcaa gactttattg aaatcagcaa caccgattct caatgaaggc    660

acatacttct tctgcaacat tcacttgacg cctaaagttg gtgagaaatg gaccgacaag    720

acatattctg ctatccacgg actgttgcct gtgtcggtgg ctacaatacg tgagtcagaa    780

gggctgacgg tggtggttcc caaggaaaag gtcgacgagt atctgtctga ctcgtcattg    840

ccgcctttgg agtacgactc caactatgag tgtgcttgga tcactttgac gatacattct    900

tcgttggagg ctgtgggtct gacagctgcg ttttcggcgc ggttggccga caacaatatc    960

agctgcaacg tcattgctgg ctttcatcat gatcacattt ttgtcggcaa aggcgacgcc   1020

cagagagcca ttgacgttct ttctaatttg gaccgatagc cgtatagtcc agtctatcta   1080

taagttcaac taactcgtaa ctattaccat aacatatact tcactgcccc agataaggtt   1140

ccgataaaaa gttctgcaga ctaaatttat ttcagtctcc tcttcaccac caaa atg     1197
                                                            Met         
                                                            1           

ccc tcc tac gaa gct cga gct aac gtc cac aag tcc gcc ttt gcc gct     1245
Pro Ser Tyr Glu Ala Arg Ala Asn Val His Lys Ser Ala Phe Ala Ala         
            5                   10                  15                  

cga gtg ctc aag ctc gtg gca gcc aag aaa acc aac ctg tgt gct tct     1293
Arg Val Leu Lys Leu Val Ala Ala Lys Lys Thr Asn Leu Cys Ala Ser         
        20                  25                  30                      

ctg gat gtt acc acc acc aag gag ctc att gag ctt gcc gat aag gtc     1341
Leu Asp Val Thr Thr Thr Lys Glu Leu Ile Glu Leu Ala Asp Lys Val         
    35                  40                  45                          

gga cct tat gtg tgc atg atc aaa acc cat atc gac atc att gac gac     1389
Gly Pro Tyr Val Cys Met Ile Lys Thr His Ile Asp Ile Ile Asp Asp         
50                  55                  60                  65          

ttc acc tac gcc ggc act gtg ctc ccc ctc aag gaa ctt gct ctt aag     1437
Phe Thr Tyr Ala Gly Thr Val Leu Pro Leu Lys Glu Leu Ala Leu Lys         
                70                  75                  80              

cac ggt ttc ttc ctg ttc gag gac aga aag ttc gca gat att ggc aac     1485
His Gly Phe Phe Leu Phe Glu Asp Arg Lys Phe Ala Asp Ile Gly Asn         
            85                  90                  95                  

act gtc aag cac cag tac cgg tgt cac cga atc gcc gag tgg tcc gat     1533
Thr Val Lys His Gln Tyr Arg Cys His Arg Ile Ala Glu Trp Ser Asp         
        100                 105                 110                     

atc acc aac gcc cac ggt gta ccc gga acc gga atc att gct ggc ctg     1581
Ile Thr Asn Ala His Gly Val Pro Gly Thr Gly Ile Ile Ala Gly Leu         
    115                 120                 125                         

cga gct ggt gcc gag gaa act gtc tct gaa cag aag aag gag gac gtc     1629
Arg Ala Gly Ala Glu Glu Thr Val Ser Glu Gln Lys Lys Glu Asp Val         
130                 135                 140                 145         

tct gac tac gag aac tcc cag tac aag gag ttc cta gtc ccc tct ccc     1677
Ser Asp Tyr Glu Asn Ser Gln Tyr Lys Glu Phe Leu Val Pro Ser Pro         
                150                 155                 160             

aac gag aag ctg gcc aga ggt ctg ctc atg ctg gcc gag ctg tct tgc     1725
Asn Glu Lys Leu Ala Arg Gly Leu Leu Met Leu Ala Glu Leu Ser Cys         
            165                 170                 175                 

aag ggc tct ctg gcc act ggc gag tac tcc aag cag acc att gag ctt     1773
Lys Gly Ser Leu Ala Thr Gly Glu Tyr Ser Lys Gln Thr Ile Glu Leu         
        180                 185                 190                     

gcc cga tcc gac ccc gag ttt gtg gtt ggc ttc att gcc cag aac cga     1821
Ala Arg Ser Asp Pro Glu Phe Val Val Gly Phe Ile Ala Gln Asn Arg         
    195                 200                 205                         

cct aag ggc gac tct gag gac tgg ctt att ctg acc ccc ggg gtg ggt     1869
Pro Lys Gly Asp Ser Glu Asp Trp Leu Ile Leu Thr Pro Gly Val Gly         
210                 215                 220                 225         

ctt gac gac aag gga gac gct ctc gga cag cag tac cga act gtt gag     1917
Leu Asp Asp Lys Gly Asp Ala Leu Gly Gln Gln Tyr Arg Thr Val Glu         
                230                 235                 240             

gat gtc atg tct acc gga acg gat atc ata att gtc ggc cga ggt ctg     1965
Asp Val Met Ser Thr Gly Thr Asp Ile Ile Ile Val Gly Arg Gly Leu         
            245                 250                 255                 

tac ggc cag aac cga gat cct att gag gag gcc aag cga tac cag aag     2013
Tyr Gly Gln Asn Arg Asp Pro Ile Glu Glu Ala Lys Arg Tyr Gln Lys         
        260                 265                 270                     

gct ggc tgg gag gct tac cag aag att aac tgt tag aggttagact          2059
Ala Gly Trp Glu Ala Tyr Gln Lys Ile Asn Cys                             
    275                 280                                             

atggatatgt aatttaactg tgtatataga gagcgtgcaa gtatggagcg cttgttcagc   2119

ttgtatgatg gtcagacgac ctgtctgatc gagtatgtat gatactgcac aacctgtgta   2179

tccgcatgat ctgtccaatg gggcatgttg ttgtgtttct cgatacggag atgctgggta   2239

cagtgctaat acgttgaact acttatactt atatgaggct cgaagaaagc tgacttgtgt   2299

atgacttatt ctcaactaca tccccagtca caataccacc actgcactac cactacacca   2359

aaaccatgat caaaccaccc atggacttcc tggaggcaga agaacttgtt atggaaaagc   2419

tcaagagaga gaagccaaga tactatcaag acatgtgtcg caacttcaag gaggaccaag   2479

ctctgtacac cgagaaacag gcctttgtcg acgaagctct aaagctgaaa cgggacatcg   2539

aaaagttgga gaagtcaatc tggaaactac acgtagaaaa cagacaccat ctccaacaag   2599

tacaggactt caaatttgca ctgaagacat tctgtgggtg gagccataag taacaatcgc   2659

ctgctccagg aacttaaaaa agaggcatta taggcccctt catctgaata ttctttgagt   2719

accaactgcc gccaactccc caatcgttaa ttagtgttat ctattctatc tatgctatta   2779

gcacatattt atcatgtgct gctgttatgt acgggttcaa tatacaaatg cttagtcctc   2839

aaacaccttc ataggtgggt cgtcttcctg gccaaatcga attctcatga tttcaaacac   2899

gaacagaccg atagacgtag acggaagcgc actgagggtc gtccagccac aaccatggta   2959

taggtatctg ggccaactca accgcagacc ctttcggatg ttattggctg tatgcagagt   3019

ctcaacgtag gctcggtagt agagatggat acccgggaag attctgtatc taagagtagt   3079

aaaacatcta atagaaaagt tgacggtctt actccatccc ttaccaaact tcacaagaag   3139

tgaatgcgtt ctgggaagag tctcctccag cgtttcagac agttccacca taagagggtt   3199

tctgtggtga atccgacgct cgtagcatag aaatgttgac tggaaaggag aggcgggact   3259

ggcctgatgt tttattcgct ctccaagttt gttctgtgtc ttttgctcct tggatcggtt   3319

agcttccagt tgttttcttg cgtcaatgag gtcttgagta tcgagacgag tcaggtagat   3379

gccctgcagc ttctgaagag gaaactgaac cacatgcagt gagatggtgg ctgctgctcc   3439

agctacaagg atgctgaaag gtgctaggac cttgttgacg atgctttttc cattctcatc   3499

aaagcctgaa ggatgttcct tgagacgatc agcatacagc cacttggcat aggacctgta   3559

ccacgatccc ttaaccattt cgaacgtggc aaaaaagacc ccaaaaccca gactctcttt   3619

gacaactgac aacgtaaagc cagcaaagac acctggtgca cctgtttggc gcaacttctc   3679

cattccatag gaccacatag agggcaactg tttggtcttg agcacttctg ctatcgaaga   3739

acgcctgtag atggcgtcga ttggtgctgc agctactgcc tgggctgctc cagcgactgc   3799

tccggcacaa aacgtctgtt tgaacgtgta tcgatgaggc atgagttcat cgatgatgta   3859

tttcacatgc gagccgtcca ttccctgggt atcataccat gatttcctta gcagcagttg   3919

tcgttggtgt tcgtcttctt catcctcctt ttgtcggtct ttctctgatt tgaataatgg   3979

tagacatccc agatatgtgg agtacaatac cagtcctacg gccgagttcg ccaataaggg   4039

gggcagcaat tgatttggaa taaaacccca gccatgctgt ttgactgcgt tggcaatcag   4099

agccggactg ctgtgtgttt tgaagctcca aggtttgtcg ctgtttggag acagcgcccg   4159

tggaagcgct aagtagtcga ctctggtagg tcgaaagagc ttgacaggca cacggatgta   4219

aaatgtgaag agctgggatg cgagcgcccg agtaccggcc gtcgtagctc caataatccg   4279

ggccgagctt ggtttccatg cttcgttatt gttttcgtgc ggttcggtga attccggaac   4339

ctcgggcaga aatcccggaa cgtcgccggg ctcgattttc tgcatgaggc ctagaaacac   4399

tctccagtgt cgcgggacgt gaggaacggg cttctatctg ctgagcgata tcaggtactg   4459

gtcgtgcttg cggttgtcca gagatccaaa acttttgcga tgcgtcaggg acgcacagtt   4519

taccgacaac ggaggagtgc aaaacgtggg ggaacagggc agtttgtgtg ggtgggtcgg   4579

cgagtccgaa tgatgatttt gtatcgttac agattgcgtg gggtggagat taatatggct   4639

gctcgaaaat tgctcgtgtc gcgttactga gaggcagagt acatgtacgc gagctctcct   4699

gtgtctactc cttcacctct ttaatgttgc tgtacatata cgcggtatga tagatgtggt   4759

caggtggtgg atttgggaat gaaggtcggt ttgggctcgg tagtggtaat gagtgtcatg   4819

ggtgaggctt aagtggcgag agatc                                         4844


<210>  71
<211>  284
<212>  PRT
<213>  Yarrowia lipolytica (GenBank Accession No. AJ306421)

<400>  71

Met Pro Ser Tyr Glu Ala Arg Ala Asn Val His Lys Ser Ala Phe Ala 
1               5                   10                  15      


Ala Arg Val Leu Lys Leu Val Ala Ala Lys Lys Thr Asn Leu Cys Ala 
            20                  25                  30          


Ser Leu Asp Val Thr Thr Thr Lys Glu Leu Ile Glu Leu Ala Asp Lys 
        35                  40                  45              


Val Gly Pro Tyr Val Cys Met Ile Lys Thr His Ile Asp Ile Ile Asp 
    50                  55                  60                  


Asp Phe Thr Tyr Ala Gly Thr Val Leu Pro Leu Lys Glu Leu Ala Leu 
65                  70                  75                  80  


Lys His Gly Phe Phe Leu Phe Glu Asp Arg Lys Phe Ala Asp Ile Gly 
                85                  90                  95      


Asn Thr Val Lys His Gln Tyr Arg Cys His Arg Ile Ala Glu Trp Ser 
            100                 105                 110         


Asp Ile Thr Asn Ala His Gly Val Pro Gly Thr Gly Ile Ile Ala Gly 
        115                 120                 125             


Leu Arg Ala Gly Ala Glu Glu Thr Val Ser Glu Gln Lys Lys Glu Asp 
    130                 135                 140                 


Val Ser Asp Tyr Glu Asn Ser Gln Tyr Lys Glu Phe Leu Val Pro Ser 
145                 150                 155                 160 


Pro Asn Glu Lys Leu Ala Arg Gly Leu Leu Met Leu Ala Glu Leu Ser 
                165                 170                 175     


Cys Lys Gly Ser Leu Ala Thr Gly Glu Tyr Ser Lys Gln Thr Ile Glu 
            180                 185                 190         


Leu Ala Arg Ser Asp Pro Glu Phe Val Val Gly Phe Ile Ala Gln Asn 
        195                 200                 205             


Arg Pro Lys Gly Asp Ser Glu Asp Trp Leu Ile Leu Thr Pro Gly Val 
    210                 215                 220                 


Gly Leu Asp Asp Lys Gly Asp Ala Leu Gly Gln Gln Tyr Arg Thr Val 
225                 230                 235                 240 


Glu Asp Val Met Ser Thr Gly Thr Asp Ile Ile Ile Val Gly Arg Gly 
                245                 250                 255     


Leu Tyr Gly Gln Asn Arg Asp Pro Ile Glu Glu Ala Lys Arg Tyr Gln 
            260                 265                 270         


Lys Ala Gly Trp Glu Ala Tyr Gln Lys Ile Asn Cys 
        275                 280                 


<210>  72
<211>  5194
<212>  DNA
<213>  Yarrowia lipolytica (GenBank Accession No. AF260230)


<220>
<221>  CDS
<222>  (798)..(2015)
<223>  Leu2

<400>  72
tcgacaccat atcatataaa actaacaatg cattgcttat tacgaagact acccgttgct     60

atctccacac cgttatctcc acggtccaaa ggctgctcaa tgtgctgcat acgtaacgtg    120

gggtgcaacc ttgagcacat agtacttttc cgaaaaccgg cgataattaa gtgtgcactc    180

caacttttca cactgagcgt aaaatgtgga gaagaaatcg gcactaaaaa gtcaggtaga    240

ctggaaaatg cgccatgaaa tgaatatctc ttgctacagt aatgcccagc atcgaggggt    300

attgtgtcac caacactata gtggcagctg aagcgctcgt gattgtagta tgagtcttta    360

ttggtgatgg gaagagttca ctcaatattc tcgttactgc caaaacacca cggtaatcgg    420

ccagacacca tggatgtaga tcaccaagcc tgtgaatgtt attcgagcta aaatgcacat    480

ggttggtgaa aggagtagtt gctgtcgaat tccgtcgtcg cctgagtcat catttattta    540

ccagttggcc acaaaccctt gacgatctcg tatgtcccct ccgacatact cccggccggc    600

tggggtacgt tcgatagcgc tatcggcatc gacaaggttt gggtccctag ccgataccgc    660

actacctgag tcacaatctt cggaggttta gtcttccaca tagcacgggc aaaagtgcgt    720

atatatacaa gagcgtttgc cagccacaga ttttcactcc acacaccaca tcacacatac    780

aaccacacac atccaca atg gaa ccc gaa act aag aag acc aag act gac       830
                   Met Glu Pro Glu Thr Lys Lys Thr Lys Thr Asp          
                   1               5                   10               

tcc aag aag att gtt ctt ctc ggc ggc gac ttc tgt ggc ccc gag gtg      878
Ser Lys Lys Ile Val Leu Leu Gly Gly Asp Phe Cys Gly Pro Glu Val         
            15                  20                  25                  

att gcc gag gcc gtc aag gtg ctc aag tct gtt gct gag gcc tcc ggc      926
Ile Ala Glu Ala Val Lys Val Leu Lys Ser Val Ala Glu Ala Ser Gly         
        30                  35                  40                      

acc gag ttt gtg ttt gag gac cga ctc att gga gga gct gcc att gag      974
Thr Glu Phe Val Phe Glu Asp Arg Leu Ile Gly Gly Ala Ala Ile Glu         
    45                  50                  55                          

aag gag ggc gag ccc atc acc gac gct act ctc gac atc tgc cga aag     1022
Lys Glu Gly Glu Pro Ile Thr Asp Ala Thr Leu Asp Ile Cys Arg Lys         
60                  65                  70                  75          

gct gac tct att atg ctc ggt gct gtc gga ggc gct gcc aac acc gta     1070
Ala Asp Ser Ile Met Leu Gly Ala Val Gly Gly Ala Ala Asn Thr Val         
                80                  85                  90              

tgg acc act ccc gac gga cga acc gac gtg cga ccc gag cag ggt ctc     1118
Trp Thr Thr Pro Asp Gly Arg Thr Asp Val Arg Pro Glu Gln Gly Leu         
            95                  100                 105                 

ctc aag ctg cga aag gac ctg aac ctg tac gcc aac ctg cga ccc tgc     1166
Leu Lys Leu Arg Lys Asp Leu Asn Leu Tyr Ala Asn Leu Arg Pro Cys         
        110                 115                 120                     

cag ctg ctg tcg ccc aag ctc gcc gat ctc tcc ccc atc cga aac gtt     1214
Gln Leu Leu Ser Pro Lys Leu Ala Asp Leu Ser Pro Ile Arg Asn Val         
    125                 130                 135                         

gag ggc acc gac ttc atc att gtc cga gag ctc gtc gga ggt atc tac     1262
Glu Gly Thr Asp Phe Ile Ile Val Arg Glu Leu Val Gly Gly Ile Tyr         
140                 145                 150                 155         

ttt gga gag cga aag gag gat gac gga tct ggc gtc gct tcc gac acc     1310
Phe Gly Glu Arg Lys Glu Asp Asp Gly Ser Gly Val Ala Ser Asp Thr         
                160                 165                 170             

gag acc tac tcc gtt cct gag gtt gag cga att gcc cga atg gcc gcc     1358
Glu Thr Tyr Ser Val Pro Glu Val Glu Arg Ile Ala Arg Met Ala Ala         
            175                 180                 185                 

ttc ctg gcc ctt cag cac aac ccc cct ctt ccc gtg tgg tct ctt gac     1406
Phe Leu Ala Leu Gln His Asn Pro Pro Leu Pro Val Trp Ser Leu Asp         
        190                 195                 200                     

aag gcc aac gtg ctg gcc tcc tct cga ctt tgg cga aag act gtc act     1454
Lys Ala Asn Val Leu Ala Ser Ser Arg Leu Trp Arg Lys Thr Val Thr         
    205                 210                 215                         

cga gtc ctc aag gac gaa ttc ccc cag ctc gag ctc aac cac cag ctg     1502
Arg Val Leu Lys Asp Glu Phe Pro Gln Leu Glu Leu Asn His Gln Leu         
220                 225                 230                 235         

atc gac tcg gcc gcc atg atc ctc atc aag cag ccc tcc aag atg aat     1550
Ile Asp Ser Ala Ala Met Ile Leu Ile Lys Gln Pro Ser Lys Met Asn         
                240                 245                 250             

ggt atc atc atc acc acc aac atg ttt ggc gat atc atc tcc gac gag     1598
Gly Ile Ile Ile Thr Thr Asn Met Phe Gly Asp Ile Ile Ser Asp Glu         
            255                 260                 265                 

gcc tcc gtc atc ccc ggt tct ctg ggt ctg ctg ccc tcc gcc tct ctg     1646
Ala Ser Val Ile Pro Gly Ser Leu Gly Leu Leu Pro Ser Ala Ser Leu         
        270                 275                 280                     

gct tct ctg ccc gac acc aac gag gcg ttc ggt ctg tac gag ccc tgt     1694
Ala Ser Leu Pro Asp Thr Asn Glu Ala Phe Gly Leu Tyr Glu Pro Cys         
    285                 290                 295                         

cac gga tct gcc ccc gat ctc ggc aag cag aag gtc aac ccc att gcc     1742
His Gly Ser Ala Pro Asp Leu Gly Lys Gln Lys Val Asn Pro Ile Ala         
300                 305                 310                 315         

acc att ctg tct gcc gcc atg atg ctc aag ttc tct ctt aac atg aag     1790
Thr Ile Leu Ser Ala Ala Met Met Leu Lys Phe Ser Leu Asn Met Lys         
                320                 325                 330             

ccc gcc ggt gac gct gtt gag gct gcc gtc aag gag tcc gtc gag gct     1838
Pro Ala Gly Asp Ala Val Glu Ala Ala Val Lys Glu Ser Val Glu Ala         
            335                 340                 345                 

ggt atc act acc gcc gat atc gga ggc tct tcc tcc acc tcc gag gtc     1886
Gly Ile Thr Thr Ala Asp Ile Gly Gly Ser Ser Ser Thr Ser Glu Val         
        350                 355                 360                     

gga gac ttg ttg cca aca agg tca agg agc tgc tca aga agg agt aag     1934
Gly Asp Leu Leu Pro Thr Arg Ser Arg Ser Cys Ser Arg Arg Ser Lys         
    365                 370                 375                         

tcg ttt cta cga cgc att gat gga agg agc aaa ctg acg cgc ctg cgg     1982
Ser Phe Leu Arg Arg Ile Asp Gly Arg Ser Lys Leu Thr Arg Leu Arg         
380                 385                 390                 395         

gtt ggt cta ccg gca ggg tcc gct agt gta taa gactctataa aaagggccct   2035
Val Gly Leu Pro Ala Gly Ser Ala Ser Val                                 
                400                 405                                 

gccctgctaa tgaaatgatg atttataatt taccggtgta gcaaccttga ctagaagaag   2095

cagattgggt gtgtttgtag tggaggacag tggtacgttt tggaaacagt cttcttgaaa   2155

gtgtcttgtc tacagtatat tcactcataa cctcaatagc caagggtgta gtcggtttat   2215

taaaggaagg gagttgtggc tgatgtggat agatatcttt aagctggcga ctgcacccaa   2275

cgagtgtggt ggtagcttgt tactgtatat tcggtaagat atattttgtg gggttttagt   2335

ggtgtttggt aggttagtgc ttggtatatg agttgtaggc atgacaattt ggaaaggggt   2395

ggactttggg aatattgtgg gatttcaata ccttagtttg tacagggtaa ttgttacaaa   2455

tgatacaaag aactgtattt cttttcattt gttttaattg gttgtatatc aagtccgtta   2515

gacgagctca gtgccatggc ttttggcact gtatttcatt tttagaggta cactacattc   2575

agtgaggtat ggtaaggttg agggcataat gaaggcacct tgtactgaca gtcacagacc   2635

tctcaccgag aattttatga gatatactcg ggttcatttt aggctccgat tcgattcaaa   2695

ttattactgt cgaaatcggt tgagcatccg ttgatttccg aacagatcta ctcatcgctt   2755

ctctccatta tcctaatgtt tgatccattt agagtgcaag caccaaagtc ggttcagtac   2815

agtaaccata ctgcaacaac caagttgtca aggctgtctc cctcggcttt gtaacgccag   2875

gcgtttctgt tttcgtccac tgcaaccatg cagtggcacg acagaggcgt gtggtctgta   2935

cagtgcattc agttcgtttt tgtatcctta cgtggcctgt cagattgaaa gatttttcag   2995

atcacccctt cgctggttct ttgttatgat acgtatgaga tgagtagagc tatggggtgt   3055

gtgttttagg accctttctc gacgactaaa tttgctattt ccccacatta aaacaactaa   3115

cctcgataaa gtggacttca cgtgtgtaag aaggctacaa gagcgagtac atacgagata   3175

tgccgtgtgc ttcttgtact gcctgttgca ctgcttgttg tacttcctcg tactgcttgt   3235

tgcactgtat gtgggggtgg tgttgttgta ctgtagacgt attgaccttc cttgtacggc   3295

tccttgtatg gctcgtattt atatgcgtct ctttttttgt tcttggctct acccctaata   3355

ctcgtcctta catgacgctt tagttgacgt cttgtgggtg tctatctcat cgggtttagc   3415

tggtcatccc cctgcaccac cgagcaacaa tagctaccgg catgtgagac actggaggca   3475

gaggcagaga aaaagcaaag ggtaaagtga gcaaggagtt tcacggattt ggcttaatag   3535

gatgtggcgt tccactgatg acttttgtct ctctagagcg gttgagaata gtttggcact   3595

ggtctttggc gttggtagcg ttctcacaca ctgtccaagt tgacgtcagc ggccgcagcc   3655

tctgtttcag cctcaaaaca ggttctattg ttgccattgt taccctgtct cctatctgat   3715

gctctccctt gtatcgagca aataagacaa tttaacgtgc atacaaaggg ctattgttgg   3775

ctcgagttga ttggccagtt tggggcaaca atggtagctg atccagggtc cttgagttca   3835

aaatgggggg gaaaccatgt acccagcacg acaagtttct ctcgggtgcc cccatcaact   3895

ctttccacaa atacatccaa gattgctgtg gatgtggctg cgtgggagat gggagttgca   3955

gtgtggttgc cgaaagcggc agcgatgtgc atcttgtggc aagagagtta aaaaaaaaaa   4015

agatagaaaa aaaagggtta cggcgaaaag aaatattaag tggtccgact gggtcgtgtg   4075

gtgcactttg ttaggaaaga cggaatgtaa atcagtcgtc agtttgggtg tttaaaccgg   4135

tgcagagaga acatcaactc tcacgccccc tcccctccgc agttgatgtc tgtccaaacc   4195

attaacccct tgataaattg tcattttgat aacaagatat gccactaatg ctgtaaacca   4255

aattcgacat ctttattcag acacctgtcc gttgtccaca aaaatgcttt taccccactg   4315

taaggacaaa acgtagagtg taccagaggt tgactttctg tctttaagct cagacttggt   4375

gtggagaagg acaaggttcc agcactctga catgacccca gcatcattct aatctctttt   4435

ggatcaagtt taacatactc ttggaatatc tatagcaccc agagaccttg acttctgcct   4495

caacactcgc cccttcgacc atgggaaacg cagaatccaa accccggcgc aagcagcggt   4555

ccacgggcag cgaaagctcc caaaccagca cccagtcgct tccggcacag actcaaacac   4615

aaacacattc cgccgccatc aaagcctcct ctcgccccaa acgaacacag agacccgcct   4675

ctgcgcaccc cgcagtaccc atccccacaa agtcttttca ggagaaatca gctctcatga   4735

accgacttgt taccccggcc cagccctgct ctgctcgggt tacttacatc aagcctgtca   4795

ccgtgtctcc tgacgacgaa aaggacgccg atattggccc caccgcaaaa gctccggtgg   4855

ttgttgcgac agaaatccct tccattctga cgccagaaga ctcgcccaaa tacctggagc   4915

cctctgaggc agcgcccttc ggtaagctcc acgtgggagc ccaagtcgcc gagggtctcc   4975

agggaaaccg acgagactca gataatcgca acattgtcaa ctccaagatc cagctgcgag   5035

atgactcgcc cgcagaagac gacgaagacg actcagaaga ctactactac aaaaacaacc   5095

tcacctacgg cctcaaacag ctcaagctag gcaccgatgt cgccaacatg aacattgagg   5155

tggtgggaca agacgccgtc tcaaagccgc tgtcgcggg                          5194


<210>  73
<211>  405
<212>  PRT
<213>  Yarrowia lipolytica (GenBank Accession No. AF260230)

<400>  73

Met Glu Pro Glu Thr Lys Lys Thr Lys Thr Asp Ser Lys Lys Ile Val 
1               5                   10                  15      


Leu Leu Gly Gly Asp Phe Cys Gly Pro Glu Val Ile Ala Glu Ala Val 
            20                  25                  30          


Lys Val Leu Lys Ser Val Ala Glu Ala Ser Gly Thr Glu Phe Val Phe 
        35                  40                  45              


Glu Asp Arg Leu Ile Gly Gly Ala Ala Ile Glu Lys Glu Gly Glu Pro 
    50                  55                  60                  


Ile Thr Asp Ala Thr Leu Asp Ile Cys Arg Lys Ala Asp Ser Ile Met 
65                  70                  75                  80  


Leu Gly Ala Val Gly Gly Ala Ala Asn Thr Val Trp Thr Thr Pro Asp 
                85                  90                  95      


Gly Arg Thr Asp Val Arg Pro Glu Gln Gly Leu Leu Lys Leu Arg Lys 
            100                 105                 110         


Asp Leu Asn Leu Tyr Ala Asn Leu Arg Pro Cys Gln Leu Leu Ser Pro 
        115                 120                 125             


Lys Leu Ala Asp Leu Ser Pro Ile Arg Asn Val Glu Gly Thr Asp Phe 
    130                 135                 140                 


Ile Ile Val Arg Glu Leu Val Gly Gly Ile Tyr Phe Gly Glu Arg Lys 
145                 150                 155                 160 


Glu Asp Asp Gly Ser Gly Val Ala Ser Asp Thr Glu Thr Tyr Ser Val 
                165                 170                 175     


Pro Glu Val Glu Arg Ile Ala Arg Met Ala Ala Phe Leu Ala Leu Gln 
            180                 185                 190         


His Asn Pro Pro Leu Pro Val Trp Ser Leu Asp Lys Ala Asn Val Leu 
        195                 200                 205             


Ala Ser Ser Arg Leu Trp Arg Lys Thr Val Thr Arg Val Leu Lys Asp 
    210                 215                 220                 


Glu Phe Pro Gln Leu Glu Leu Asn His Gln Leu Ile Asp Ser Ala Ala 
225                 230                 235                 240 


Met Ile Leu Ile Lys Gln Pro Ser Lys Met Asn Gly Ile Ile Ile Thr 
                245                 250                 255     


Thr Asn Met Phe Gly Asp Ile Ile Ser Asp Glu Ala Ser Val Ile Pro 
            260                 265                 270         


Gly Ser Leu Gly Leu Leu Pro Ser Ala Ser Leu Ala Ser Leu Pro Asp 
        275                 280                 285             


Thr Asn Glu Ala Phe Gly Leu Tyr Glu Pro Cys His Gly Ser Ala Pro 
    290                 295                 300                 


Asp Leu Gly Lys Gln Lys Val Asn Pro Ile Ala Thr Ile Leu Ser Ala 
305                 310                 315                 320 


Ala Met Met Leu Lys Phe Ser Leu Asn Met Lys Pro Ala Gly Asp Ala 
                325                 330                 335     


Val Glu Ala Ala Val Lys Glu Ser Val Glu Ala Gly Ile Thr Thr Ala 
            340                 345                 350         


Asp Ile Gly Gly Ser Ser Ser Thr Ser Glu Val Gly Asp Leu Leu Pro 
        355                 360                 365             


Thr Arg Ser Arg Ser Cys Ser Arg Arg Ser Lys Ser Phe Leu Arg Arg 
    370                 375                 380                 


Ile Asp Gly Arg Ser Lys Leu Thr Arg Leu Arg Val Gly Leu Pro Ala 
385                 390                 395                 400 


Gly Ser Ala Ser Val 
                405 


<210>  74
<211>  2569
<212>  DNA
<213>  Yarrowia lipolytica (GenBank Accession No. M34929)


<220>
<221>  misc_feature
<223>  Lys5 coding sequence is complement of 655..1764

<400>  74
gagtctggaa cggtccctct tctcagaatt gtgaacgaaa ggaacaggtt caacgaacag     60

aacagattcc atagccacac ctttgcctat ggcttcacaa ccgaaggcaa ttcgagaggt    120

cgcgcttatg gaatcgactc gtataaagct gaagggaaag ggagacgttc cgagcgctca    180

gatgcaatag tcgtccagct aatgtggatt caaaaacaac cccaacagta atcttgaaaa    240

tttgaacgga tcaatctgaa cactcttgct ccaggtcatt cttctaacgc acatccccag    300

agtctagagg gagttgtgtt gtgaacatcc taataaacaa tgcaatggat tcgggatatc    360

ttctgtctcg ccccctactc gatgtcgagt aaaccgatca ccaactaaca atactcctcc    420

gcgttctgcc attgactctc aaacagacat cgctatcaac ggaacagcat attttagctt    480

cttaggacaa taaatattga taatgccggc tctccctcgg tatattaagc aatccattca    540

tacactcatt catcaggtta attttatata tataatttgt ctattcaaac accgtaaatt    600

actggtacca tcatctcctc cttttcaaat acacgtctat ttgcattaat gaaattactc    660

gccaattcgc agaacgtgtt tgtcgaacag agccttagct cgggtccaga caggagcagt    720

gtctcgctga ggaagctgca ggagagaagg cagcagagcc tcagaaaagg cctcggaagc    780

ctctcgcggc agaagagagg gcaggtggtc gatcgagcac acggacagct tggggccagc    840

agtggtctca acaggcacgg tgggatggtc gaacgtggta gcaattgtgt acacggggac    900

agggttgtgg gggttggtgg tgtcagcaga gacgtcgaca atgacactga gctttcgggt    960

ctccttgttg agcagatcgt agttgatgaa aggaggaatg ggctgagaca ggtagatgca   1020

gttgatgaag atatccgcgt cagcaatctc ttggaagggt ccacccttct tggtctcgtt   1080

catgtcccat cgaatgatgt tctcttcggg gataccgacc tttcgggcca gatcaatggc   1140

accggatcca catcggccaa gagcaccaat caccaggacg gtagggagct tggagccctt   1200

ctcaacagca gcggcaagat ccttcttaat cttgtcgaca agctcggtct cattgggata   1260

ggcagagaca ccgggcaggt tttcgctgtc ggggtgggtc tgctggaagg cccaagtctc   1320

gacaccgatg gcggcaccgg cgaatccagc gtggaagcca aaggcggcaa ctcgtcgtcc   1380

attgtcatcc tccaggaact caaggtcgta cagagttccg tttcctgcgg ggaatcggct   1440

cagaacgtcc ttccatccgc cctgatcctt gtagcagtga gcaaactgga tgtgctcgtg   1500

agacagaggg aaagattcct caggcagctc cttaagacca ataatcattc ggtcctcggg   1560

ggcagagacc caagagccct cctcgacaag agtggctccg acatcgacaa actcctggtc   1620

gtcgaagatt cgaaggggag acttctccac aaagacctcg aatccagcat caagcagctt   1680

tcgggtggta gtaggcgtga gagcagatcg gtgctcgaga ggcttggtct cggctcggag   1740

atggagcttc actggtgcgg tcattgtgtc tgtgttgttg tatgtggtga agtgacaatg   1800

gatggtgtgt cgtatcaggc tggggaacaa ttgtgcttaa gtatgctgca gttgagtaag   1860

agtcatcgat ccaccaaaat aaagtttgcc attagggttg gagagagaga tggtggctgg   1920

aagaattaaa tgacatcaag ctgaggattg tgggtgtgca ataacacatg ttaggggtga   1980

cctgtggctc gaaatctgat aattattttg taactttatg attattctta gattttttaa   2040

tattcctcta tataacacat aagtagctgt cgtctagttg ttcatagcct gactcctgca   2100

atagattagt gcagagtgat tttgtgcaat tgagagccac ggttgagtca agtgactttg   2160

tgtgtgaagt catcttacgt ttcaagtctc acaggttact caattggttg gttgtctgcc   2220

ctttacagat atttacagta cctgagcgta aagtcgttca tccacggaat gactgttcct   2280

gtcacgcagt catgatcatg gatgtggctg gtcaggaacc attttggata ggagacttag   2340

ggattggact attattgaaa aaactgagcc gaatatgata tagttctatt tgaatgcaga   2400

acttctgatg gtcaattcac ttatttcagg catatcggtc atggtggcag ctgccacgat   2460

gttatctcgt tggaaacctc gtccgccatt aaccggaaca tgcttctaga agatgagaat   2520

acagcatgta cttgtacaag attctcggca ttgatggtct caggcatgc               2569


<210>  75
<211>  677
<212>  PRT
<213>  Yarrowia lipolytica (GenBank Accession No. XP_504703)


<220>
<221>  MISC_FEATURE
<222>  (1)..(677)
<223>  Pox1

<400>  75

Met Ala Lys Glu Arg Gly Lys Thr Gln Phe Thr Val Arg Asp Val Thr 
1               5                   10                  15      


Asn Phe Leu Asn Gly Gly Glu Glu Glu Thr Gln Ile Val Glu Lys Ile 
            20                  25                  30          


Met Ser Ser Ile Glu Arg Asp Pro Val Leu Ser Val Thr Ala Asp Tyr 
        35                  40                  45              


Asp Cys Asn Leu Gln Gln Ala Arg Lys Gln Thr Met Glu Arg Val Ala 
    50                  55                  60                  


Ala Leu Ser Pro Tyr Leu Val Thr Asp Thr Glu Lys Leu Ser Leu Trp 
65                  70                  75                  80  


Arg Ala Gln Leu His Gly Met Val Asp Met Ser Thr Arg Thr Arg Leu 
                85                  90                  95      


Ser Ile His Asn Asn Leu Phe Ile Gly Ser Ile Arg Gly Ser Gly Thr 
            100                 105                 110         


Pro Glu Gln Phe Lys Tyr Trp Val Lys Lys Gly Ala Val Ala Val Lys 
        115                 120                 125             


Gln Phe Tyr Gly Cys Phe Ala Met Thr Glu Leu Gly His Gly Ser Asn 
    130                 135                 140                 


Leu Lys Gly Leu Glu Thr Thr Ala Thr Tyr Asp Gln Asp Ser Asp Gln 
145                 150                 155                 160 


Phe Ile Ile Asn Thr Pro His Ile Gly Ala Thr Lys Trp Trp Ile Gly 
                165                 170                 175     


Gly Ala Ala His Thr Ser Thr His Cys Val Cys Phe Ala Lys Leu Ile 
            180                 185                 190         


Val His Gly Lys Asp Tyr Gly Thr Arg Asn Phe Val Val Pro Leu Arg 
        195                 200                 205             


Asn Val His Asp His Ser Leu Lys Val Gly Val Ser Ile Gly Asp Ile 
    210                 215                 220                 


Gly Lys Lys Met Gly Arg Asp Gly Val Asp Asn Gly Trp Ile Gln Phe 
225                 230                 235                 240 


Thr Asn Val Arg Ile Pro Arg Gln Asn Met Leu Met Arg Tyr Ala Lys 
                245                 250                 255     


Val Ser Asp Thr Gly Val Val Thr Lys Pro Ala Leu Asp Gln Leu Thr 
            260                 265                 270         


Tyr Gly Ala Leu Ile Arg Gly Arg Val Ser Met Ile Ala Asp Ser Phe 
        275                 280                 285             


His Val Ser Lys Arg Phe Leu Thr Ile Ala Leu Arg Tyr Ala Cys Val 
    290                 295                 300                 


Arg Arg Gln Phe Gly Thr Ser Gly Asp Thr Lys Glu Thr Lys Ile Ile 
305                 310                 315                 320 


Asp Tyr Pro Tyr His Gln Arg Arg Leu Leu Pro Leu Leu Ala Tyr Cys 
                325                 330                 335     


Tyr Ala Met Lys Met Gly Ala Asp Glu Ala Gln Lys Thr Trp Ile Glu 
            340                 345                 350         


Thr Thr Asp Arg Ile Leu Ala Leu Asn Pro Asn Asp Pro Ala Gln Lys 
        355                 360                 365             


Asn Asp Leu Glu Lys Ala Val Thr Asp Thr Lys Glu Leu Phe Ala Ala 
    370                 375                 380                 


Ser Ala Gly Met Lys Ala Phe Thr Thr Trp Gly Cys Ala Lys Ile Ile 
385                 390                 395                 400 


Asp Glu Cys Arg Gln Ala Cys Gly Gly His Gly Tyr Ser Gly Tyr Asn 
                405                 410                 415     


Gly Phe Gly Gln Gly Tyr Ala Asp Trp Val Val Gln Cys Thr Trp Glu 
            420                 425                 430         


Gly Asp Asn Asn Val Leu Cys Leu Ser Met Gly Arg Gly Leu Val Gln 
        435                 440                 445             


Ser Ala Leu Gln Ile Leu Ala Gly Lys His Val Gly Ala Ser Ile Gln 
    450                 455                 460                 


Tyr Val Gly Asp Lys Ser Lys Ile Ser Gln Asn Gly Gln Gly Thr Pro 
465                 470                 475                 480 


Arg Glu Gln Leu Leu Ser Pro Glu Phe Leu Val Glu Ala Phe Arg Thr 
                485                 490                 495     


Ala Ser Arg Asn Asn Ile Leu Arg Thr Thr Asp Lys Tyr Gln Glu Leu 
            500                 505                 510         


Val Lys Thr Leu Asn Pro Asp Gln Ala Phe Glu Glu Leu Ser Gln Gln 
        515                 520                 525             


Arg Phe Gln Cys Ala Arg Ile His Thr Arg Gln His Leu Ile Ser Ser 
    530                 535                 540                 


Phe Tyr Ala Arg Ile Ala Thr Ala Lys Asp Asp Ile Lys Pro His Leu 
545                 550                 555                 560 


Leu Lys Leu Ala Asn Leu Phe Ala Leu Trp Ser Ile Glu Glu Asp Thr 
                565                 570                 575     


Gly Ile Phe Leu Arg Glu Asn Ile Leu Thr Pro Gly Asp Ile Asp Leu 
            580                 585                 590         


Ile Asn Ser Leu Val Asp Glu Leu Cys Val Ala Val Arg Asp Gln Val 
        595                 600                 605             


Ile Gly Leu Thr Asp Ala Phe Gly Leu Ser Asp Phe Phe Ile Asn Ala 
    610                 615                 620                 


Pro Ile Gly Ser Tyr Asp Gly Asn Val Tyr Glu Lys Tyr Phe Ala Lys 
625                 630                 635                 640 


Val Asn Gln Gln Asn Pro Ala Thr Asn Pro Arg Pro Pro Tyr Tyr Glu 
                645                 650                 655     


Ser Thr Leu Lys Pro Phe Leu Phe Arg Glu Glu Glu Asp Asp Glu Ile 
            660                 665                 670         


Cys Asp Leu Asp Glu 
        675         


<210>  76
<211>  700
<212>  PRT
<213>  Yarrowia lipolytica (GenBank Accession No. XP_505264)


<220>
<221>  MISC_FEATURE
<222>  (1)..(700)
<223>  Pox2

<400>  76

Met Asn Pro Asn Asn Thr Gly Thr Ile Glu Ile Asn Gly Lys Glu Tyr 
1               5                   10                  15      


Asn Thr Phe Thr Glu Pro Pro Val Ala Met Ala Gln Glu Arg Ala Lys 
            20                  25                  30          


Thr Ser Phe Pro Val Arg Glu Met Thr Tyr Phe Leu Asp Gly Gly Glu 
        35                  40                  45              


Lys Asn Thr Leu Lys Asn Glu Gln Ile Met Glu Glu Ile Glu Arg Asp 
    50                  55                  60                  


Pro Leu Phe Asn Asn Asp Asn Tyr Tyr Asp Leu Asn Lys Glu Gln Ile 
65                  70                  75                  80  


Arg Glu Leu Thr Met Glu Arg Val Ala Lys Leu Ser Leu Phe Val Arg 
                85                  90                  95      


Asp Gln Pro Glu Asp Asp Ile Lys Lys Arg Phe Ala Leu Ile Gly Ile 
            100                 105                 110         


Ala Asp Met Gly Thr Tyr Thr Arg Leu Gly Val His Tyr Gly Leu Phe 
        115                 120                 125             


Phe Gly Ala Val Arg Gly Thr Gly Thr Ala Glu Gln Phe Gly His Trp 
    130                 135                 140                 


Ile Ser Lys Gly Ala Gly Asp Leu Arg Lys Phe Tyr Gly Cys Phe Ser 
145                 150                 155                 160 


Met Thr Glu Leu Gly His Gly Ser Asn Leu Ala Gly Leu Glu Thr Thr 
                165                 170                 175     


Ala Ile Tyr Asp Glu Glu Thr Asp Glu Phe Ile Ile Asn Thr Pro His 
            180                 185                 190         


Ile Ala Ala Thr Lys Trp Trp Ile Gly Gly Ala Ala His Thr Ala Thr 
        195                 200                 205             


His Thr Val Val Phe Ala Arg Leu Ile Val Lys Gly Lys Asp Tyr Gly 
    210                 215                 220                 


Val Lys Thr Phe Val Val Gln Leu Arg Asn Ile Asn Asp His Ser Leu 
225                 230                 235                 240 


Lys Val Gly Ile Ser Ile Gly Asp Ile Gly Lys Lys Met Gly Arg Asp 
                245                 250                 255     


Gly Ile Asp Asn Gly Trp Ile Gln Phe Thr Asn Val Arg Ile Pro Arg 
            260                 265                 270         


Gln Asn Leu Leu Met Lys Tyr Thr Lys Val Asp Arg Glu Gly Asn Val 
        275                 280                 285             


Thr Gln Pro Pro Leu Ala Gln Leu Thr Tyr Gly Ser Leu Ile Thr Gly 
    290                 295                 300                 


Arg Val Ser Met Ala Ser Asp Ser His Gln Val Gly Lys Arg Phe Ile 
305                 310                 315                 320 


Thr Ile Ala Leu Arg Tyr Ala Cys Ile Arg Arg Gln Phe Ser Thr Thr 
                325                 330                 335     


Pro Gly Gln Pro Glu Thr Lys Ile Ile Asp Tyr Pro Tyr His Gln Arg 
            340                 345                 350         


Arg Leu Leu Pro Leu Leu Ala Tyr Val Tyr Ala Leu Lys Met Thr Ala 
        355                 360                 365             


Asp Glu Val Gly Ala Leu Phe Ser Arg Thr Met Leu Lys Met Asp Asp 
    370                 375                 380                 


Leu Lys Pro Asp Asp Lys Ala Gly Leu Asn Glu Val Val Ser Asp Val 
385                 390                 395                 400 


Lys Glu Leu Phe Ser Val Ser Ala Gly Leu Lys Ala Phe Ser Thr Trp 
                405                 410                 415     


Ala Cys Ala Asp Val Ile Asp Lys Thr Arg Gln Ala Cys Gly Gly His 
            420                 425                 430         


Gly Tyr Ser Gly Tyr Asn Gly Phe Gly Gln Ala Tyr Ala Asp Trp Val 
        435                 440                 445             


Val Gln Cys Thr Trp Glu Gly Asp Asn Asn Ile Leu Thr Leu Ser Ala 
    450                 455                 460                 


Gly Arg Ala Leu Ile Gln Ser Ala Val Ala Leu Arg Lys Gly Glu Pro 
465                 470                 475                 480 


Val Gly Asn Ala Val Ser Tyr Leu Lys Arg Tyr Lys Asp Leu Ala Asn 
                485                 490                 495     


Ala Lys Leu Asn Gly Arg Ser Leu Thr Asp Pro Lys Val Leu Val Glu 
            500                 505                 510         


Ala Trp Glu Val Ala Ala Gly Asn Ile Ile Asn Arg Ala Thr Asp Gln 
        515                 520                 525             


Tyr Glu Lys Leu Ile Gly Glu Gly Leu Asn Ala Asp Gln Ala Phe Glu 
    530                 535                 540                 


Val Leu Ser Gln Gln Arg Phe Gln Ala Ala Lys Val His Thr Arg Arg 
545                 550                 555                 560 


His Leu Ile Ala Ala Phe Phe Ser Arg Ile Asp Thr Glu Ala Gly Glu 
                565                 570                 575     


Ala Ile Lys Gln Pro Leu Leu Asn Leu Ala Leu Leu Phe Ala Leu Trp 
            580                 585                 590         


Ser Ile Glu Glu Asp Ser Gly Leu Phe Leu Arg Glu Gly Phe Leu Glu 
        595                 600                 605             


Pro Lys Asp Ile Asp Thr Val Thr Glu Leu Val Asn Lys Tyr Cys Thr 
    610                 615                 620                 


Thr Val Arg Glu Glu Val Ile Gly Tyr Thr Asp Ala Phe Asn Leu Ser 
625                 630                 635                 640 


Asp Tyr Phe Ile Asn Ala Pro Ile Gly Cys Tyr Asp Gly Asp Ala Tyr 
                645                 650                 655     


Arg His Tyr Phe Gln Lys Val Asn Glu Gln Asn Pro Ala Arg Asp Pro 
            660                 665                 670         


Arg Pro Pro Tyr Tyr Ala Ser Thr Leu Lys Pro Phe Leu Phe Arg Glu 
        675                 680                 685             


Glu Glu Asp Asp Asp Ile Cys Glu Leu Asp Glu Glu 
    690                 695                 700 


<210>  77
<211>  700
<212>  PRT
<213>  Yarrowia lipolytica (GenBank Accession No. XP_503244)


<220>
<221>  MISC_FEATURE
<222>  (1)..(700)
<223>  Pox3

<400>  77

Met Ile Ser Pro Asn Leu Thr Ala Asn Val Glu Ile Asp Gly Lys Gln 
1               5                   10                  15      


Tyr Asn Thr Phe Thr Glu Pro Pro Lys Ala Leu Ala Gly Glu Arg Ala 
            20                  25                  30          


Lys Val Lys Phe Pro Ile Lys Asp Met Thr Glu Phe Leu His Gly Gly 
        35                  40                  45              


Glu Glu Asn Val Thr Met Ile Glu Arg Leu Met Thr Glu Leu Glu Arg 
    50                  55                  60                  


Asp Pro Val Leu Asn Val Ser Gly Asp Tyr Asp Met Pro Lys Glu Gln 
65                  70                  75                  80  


Leu Arg Glu Thr Ala Val Ala Arg Ile Ala Ala Leu Ser Gly His Trp 
                85                  90                  95      


Lys Lys Asp Thr Glu Lys Glu Ala Leu Leu Arg Ser Gln Leu His Gly 
            100                 105                 110         


Ile Val Asp Met Gly Thr Arg Ile Arg Leu Gly Val His Thr Gly Leu 
        115                 120                 125             


Phe Met Gly Ala Ile Arg Gly Ser Gly Thr Lys Glu Gln Tyr Asp Tyr 
    130                 135                 140                 


Trp Val Arg Lys Gly Ala Ala Asp Val Lys Gly Phe Tyr Gly Cys Phe 
145                 150                 155                 160 


Ala Met Thr Glu Leu Gly His Gly Ser Asn Val Ala Gly Leu Glu Thr 
                165                 170                 175     


Thr Ala Thr Tyr Ile Gln Asp Thr Asp Glu Phe Ile Ile Asn Thr Pro 
            180                 185                 190         


Asn Thr Gly Ala Thr Lys Trp Trp Ile Gly Gly Ala Ala His Ser Ala 
        195                 200                 205             


Thr His Thr Ala Cys Phe Ala Arg Leu Leu Val Asp Gly Lys Asp Tyr 
    210                 215                 220                 


Gly Val Lys Ile Phe Val Val Gln Leu Arg Asp Val Ser Ser His Ser 
225                 230                 235                 240 


Leu Met Pro Gly Ile Ala Leu Gly Asp Ile Gly Lys Lys Met Gly Arg 
                245                 250                 255     


Asp Ala Ile Asp Asn Gly Trp Ile Gln Phe Thr Asn Val Arg Ile Pro 
            260                 265                 270         


Arg Gln Asn Met Leu Met Lys Tyr Ala Lys Val Ser Ser Thr Gly Lys 
        275                 280                 285             


Val Ser Gln Pro Pro Leu Ala Gln Leu Thr Tyr Gly Ala Leu Ile Gly 
    290                 295                 300                 


Gly Arg Val Thr Met Ile Ala Asp Ser Phe Phe Val Ser Gln Arg Phe 
305                 310                 315                 320 


Ile Thr Ile Ala Leu Arg Tyr Ala Cys Val Arg Arg Gln Phe Gly Thr 
                325                 330                 335     


Thr Pro Gly Gln Pro Glu Thr Lys Ile Ile Asp Tyr Pro Tyr His Gln 
            340                 345                 350         


Arg Arg Leu Leu Pro Leu Leu Ala Phe Thr Tyr Ala Met Lys Met Ala 
        355                 360                 365             


Ala Asp Gln Ser Gln Ile Gln Tyr Asp Gln Thr Thr Asp Leu Leu Gln 
    370                 375                 380                 


Thr Ile Asp Pro Lys Asp Lys Gly Ala Leu Gly Lys Ala Ile Val Asp 
385                 390                 395                 400 


Leu Lys Glu Leu Phe Ala Ser Ser Ala Gly Leu Lys Ala Phe Thr Thr 
                405                 410                 415     


Trp Thr Cys Ala Asn Ile Ile Asp Gln Cys Arg Gln Ala Cys Gly Gly 
            420                 425                 430         


His Gly Tyr Ser Gly Tyr Asn Gly Phe Gly Gln Ala Tyr Ala Asp Trp 
        435                 440                 445             


Val Val Gln Cys Thr Trp Glu Gly Asp Asn Asn Val Leu Cys Leu Ser 
    450                 455                 460                 


Met Gly Arg Gly Leu Ile Gln Ser Cys Leu Gly His Arg Lys Gly Lys 
465                 470                 475                 480 


Pro Leu Gly Ser Ser Val Gly Tyr Leu Ala Asn Lys Gly Leu Glu Gln 
                485                 490                 495     


Ala Thr Leu Ser Gly Arg Asp Leu Lys Asp Pro Lys Val Leu Ile Glu 
            500                 505                 510         


Ala Trp Glu Lys Val Ala Asn Gly Ala Ile Gln Arg Ala Thr Asp Lys 
        515                 520                 525             


Phe Val Glu Leu Thr Lys Gly Gly Leu Ser Pro Asp Gln Ala Phe Glu 
    530                 535                 540                 


Glu Leu Ser Gln Gln Arg Phe Gln Cys Ala Lys Ile His Thr Arg Lys 
545                 550                 555                 560 


His Leu Val Thr Ala Phe Tyr Glu Arg Ile Asn Ala Ser Ala Lys Ala 
                565                 570                 575     


Asp Val Lys Pro Tyr Leu Ile Asn Leu Ala Asn Leu Phe Thr Leu Trp 
            580                 585                 590         


Ser Ile Glu Glu Asp Ser Gly Leu Phe Leu Arg Glu Gly Phe Leu Gln 
        595                 600                 605             


Pro Lys Asp Ile Asp Gln Val Thr Glu Leu Val Asn His Tyr Cys Lys 
    610                 615                 620                 


Glu Val Arg Asp Gln Val Ala Gly Tyr Thr Asp Ala Phe Gly Leu Ser 
625                 630                 635                 640 


Asp Trp Phe Ile Asn Ala Pro Ile Gly Asn Tyr Asp Gly Asp Val Tyr 
                645                 650                 655     


Lys His Tyr Phe Ala Lys Val Asn Gln Gln Asn Pro Ala Gln Asn Pro 
            660                 665                 670         


Arg Pro Pro Tyr Tyr Glu Ser Thr Leu Arg Pro Phe Leu Phe Arg Glu 
        675                 680                 685             


Asp Glu Asp Asp Asp Ile Cys Glu Leu Asp Glu Glu 
    690                 695                 700 


<210>  78
<211>  701
<212>  PRT
<213>  Yarrowia lipolytica (GenBank Accession No. XP_504475)


<220>
<221>  MISC_FEATURE
<222>  (1)..(701)
<223>  Pox4

<400>  78

Met Ile Thr Pro Asn Pro Ala Asn Asp Ile Val His Asp Gly Lys Leu 
1               5                   10                  15      


Tyr Asp Thr Phe Thr Glu Pro Pro Lys Leu Met Ala Gln Glu Arg Ala 
            20                  25                  30          


Gln Leu Asp Phe Asp Pro Arg Asp Ile Thr Tyr Phe Leu Asp Gly Ser 
        35                  40                  45              


Lys Glu Glu Thr Glu Leu Leu Glu Ser Leu Met Leu Met Tyr Glu Arg 
    50                  55                  60                  


Asp Pro Leu Phe Asn Asn Gln Asn Glu Tyr Asp Glu Ser Phe Glu Thr 
65                  70                  75                  80  


Leu Arg Glu Arg Ser Val Lys Arg Ile Phe Gln Leu Ser Lys Ser Ile 
                85                  90                  95      


Ala Met Asp Pro Glu Pro Met Ser Phe Arg Lys Ile Gly Phe Leu Gly 
            100                 105                 110         


Ile Leu Asp Met Gly Thr Tyr Ala Arg Leu Gly Val His Tyr Ala Leu 
        115                 120                 125             


Phe Cys Asn Ser Ile Arg Gly Gln Gly Thr Pro Asp Gln Leu Met Tyr 
    130                 135                 140                 


Trp Leu Asp Gln Gly Ala Met Val Ile Lys Gly Phe Tyr Gly Cys Phe 
145                 150                 155                 160 


Ala Met Thr Glu Met Gly His Gly Ser Asn Leu Ser Arg Leu Glu Thr 
                165                 170                 175     


Ile Ala Thr Phe Asp Lys Glu Thr Asp Glu Phe Ile Ile Asn Thr Pro 
            180                 185                 190         


His Val Gly Ala Thr Lys Trp Trp Ile Gly Gly Ala Ala His Thr Ala 
        195                 200                 205             


Thr His Thr Leu Ala Phe Ala Arg Leu Gln Val Asp Gly Lys Asp Tyr 
    210                 215                 220                 


Gly Val Lys Ser Phe Val Val Pro Leu Arg Asn Leu Asp Asp His Ser 
225                 230                 235                 240 


Leu Arg Pro Gly Ile Ala Thr Gly Asp Ile Gly Lys Lys Met Gly Arg 
                245                 250                 255     


Asp Ala Val Asp Asn Gly Trp Ile Gln Phe Thr Asn Val Arg Val Pro 
            260                 265                 270         


Arg Asn Tyr Met Leu Met Lys His Thr Lys Val Leu Arg Asp Gly Thr 
        275                 280                 285             


Val Lys Gln Pro Pro Leu Ala Gln Leu Thr Tyr Gly Ser Leu Ile Thr 
    290                 295                 300                 


Gly Arg Val Gln Met Thr Thr Asp Ser His Asn Val Ser Lys Lys Phe 
305                 310                 315                 320 


Leu Thr Ile Ala Leu Arg Tyr Ala Thr Ile Arg Arg Gln Phe Ser Ser 
                325                 330                 335     


Thr Pro Gly Glu Pro Glu Thr Arg Leu Ile Asp Tyr Leu Tyr His Gln 
            340                 345                 350         


Arg Arg Leu Leu Pro Leu Met Ala Tyr Ser Tyr Ala Met Lys Leu Ala 
        355                 360                 365             


Gly Asp His Val Arg Glu Leu Phe Phe Ala Ser Gln Glu Lys Ala Glu 
    370                 375                 380                 


Ser Leu Lys Glu Asp Asp Lys Ala Gly Val Glu Ser Tyr Val Gln Asp 
385                 390                 395                 400 


Ile Lys Glu Leu Phe Ser Val Ser Ala Gly Leu Lys Ala Ala Thr Thr 
                405                 410                 415     


Trp Ala Cys Ala Asp Ile Ile Asp Lys Ala Arg Gln Ala Cys Gly Gly 
            420                 425                 430         


His Gly Tyr Ser Ala Tyr Asn Gly Phe Gly Gln Ala Phe Gln Asp Trp 
        435                 440                 445             


Val Val Gln Cys Thr Trp Glu Gly Asp Asn Thr Val Leu Thr Leu Ser 
    450                 455                 460                 


Ala Gly Arg Ala Leu Ile Gln Ser Ala Leu Val Tyr Arg Lys Glu Gly 
465                 470                 475                 480 


Lys Leu Gly Asn Ala Thr Lys Tyr Leu Ser Arg Ser Lys Glu Leu Ala 
                485                 490                 495     


Asn Ala Lys Arg Asn Gly Arg Ser Leu Glu Asp Pro Lys Leu Leu Val 
            500                 505                 510         


Glu Ala Trp Glu Ala Val Ser Ala Gly Ala Ile Asn Ala Ala Thr Asp 
        515                 520                 525             


Ala Tyr Glu Glu Leu Ser Lys Gln Gly Val Ser Val Asp Glu Cys Phe 
    530                 535                 540                 


Glu Gln Val Ser Gln Glu Arg Phe Gln Ala Ala Arg Ile His Thr Arg 
545                 550                 555                 560 


Arg Ala Leu Ile Glu Ala Phe Tyr Ser Arg Ile Ala Thr Ala Asp Glu 
                565                 570                 575     


Lys Val Lys Pro His Leu Ile Pro Leu Ala Asn Leu Phe Ala Leu Trp 
            580                 585                 590         


Ser Ile Glu Glu Asp Ser Ala Leu Phe Leu Ala Glu Gly Tyr Phe Glu 
        595                 600                 605             


Pro Glu Asp Ile Ile Glu Val Thr Ser Leu Val Asn Lys Tyr Cys Gly 
    610                 615                 620                 


Ile Val Arg Lys Asn Val Ile Gly Tyr Thr Asp Ala Phe Asn Leu Ser 
625                 630                 635                 640 


Asp Tyr Phe Ile Asn Ala Ala Ile Gly Arg Tyr Asp Gly Asp Val Tyr 
                645                 650                 655     


Lys Asn Tyr Phe Glu Lys Val Lys Gln Gln Tyr Pro Pro Glu Gly Gly 
            660                 665                 670         


Lys Pro His Tyr Tyr Glu Asp Val Met Lys Pro Phe Leu His Arg Glu 
        675                 680                 685             


Arg Ile Pro Asp Val Pro Met Glu Pro Glu Asp Ile Gln 
    690                 695                 700     


<210>  79
<211>  699
<212>  PRT
<213>  Yarrowia lipolytica (GenBank Accession No. XP_502199)


<220>
<221>  MISC_FEATURE
<222>  (1)..(699)
<223>  Pox5

<400>  79

Met Asn Asn Asn Pro Thr Asn Val Ile Leu Gly Gly Lys Glu Tyr Asp 
1               5                   10                  15      


Thr Phe Thr Glu Pro Pro Ala Gln Met Glu Leu Glu Arg Ala Lys Thr 
            20                  25                  30          


Gln Phe Lys Val Arg Asp Val Thr Asn Phe Leu Thr Gly Ser Glu Gln 
        35                  40                  45              


Glu Thr Leu Leu Thr Glu Arg Ile Met Arg Glu Ile Glu Arg Asp Pro 
    50                  55                  60                  


Val Leu Asn Val Ala Gly Asp Tyr Asp Ala Asp Leu Pro Thr Lys Arg 
65                  70                  75                  80  


Arg Gln Ala Val Glu Arg Ile Gly Ala Leu Ala Arg Tyr Leu Pro Lys 
                85                  90                  95      


Asp Ser Glu Lys Glu Ala Ile Leu Arg Gly Gln Leu His Gly Ile Val 
            100                 105                 110         


Asp Met Gly Thr Arg Thr Arg Ile Ala Val His Tyr Gly Leu Phe Met 
        115                 120                 125             


Gly Ala Ile Arg Gly Ser Gly Thr Lys Glu Gln Tyr Asp Tyr Trp Val 
    130                 135                 140                 


Ala Lys Gly Ala Ala Thr Leu His Lys Phe Tyr Gly Cys Phe Ala Met 
145                 150                 155                 160 


Thr Glu Leu Gly His Gly Ser Asn Val Ala Gly Leu Glu Thr Thr Ala 
                165                 170                 175     


Thr Leu Asp Lys Asp Thr Asp Glu Phe Ile Ile Asn Thr Pro Asn Ser 
            180                 185                 190         


Gly Ala Thr Lys Trp Trp Ile Gly Gly Ala Ala His Ser Ala Thr His 
        195                 200                 205             


Thr Ala Cys Leu Ala Arg Leu Ile Val Asp Gly Lys Asp Tyr Gly Val 
    210                 215                 220                 


Lys Ile Phe Ile Val Gln Leu Arg Asp Leu Asn Ser His Ser Leu Leu 
225                 230                 235                 240 


Asn Gly Ile Ala Ile Gly Asp Ile Gly Lys Lys Met Gly Arg Asp Ala 
                245                 250                 255     


Ile Asp Asn Gly Trp Ile Gln Phe Thr Asp Val Arg Ile Pro Arg Gln 
            260                 265                 270         


Asn Met Leu Met Arg Tyr Asp Arg Val Ser Arg Asp Gly Glu Val Thr 
        275                 280                 285             


Thr Ser Glu Leu Ala Gln Leu Thr Tyr Gly Ala Leu Leu Ser Gly Arg 
    290                 295                 300                 


Val Thr Met Ile Ala Glu Ser His Leu Leu Ser Ala Arg Phe Leu Thr 
305                 310                 315                 320 


Ile Ala Leu Arg Tyr Ala Cys Ile Arg Arg Gln Phe Gly Ala Val Pro 
                325                 330                 335     


Asp Lys Pro Glu Thr Lys Leu Ile Asp Tyr Pro Tyr His Gln Arg Arg 
            340                 345                 350         


Leu Leu Pro Leu Leu Ala Tyr Thr Tyr Ala Met Lys Met Gly Ala Asp 
        355                 360                 365             


Glu Ala Gln Gln Gln Tyr Asn Ser Ser Phe Gly Ala Leu Leu Lys Leu 
    370                 375                 380                 


Asn Pro Val Lys Asp Ala Glu Lys Phe Ala Val Ala Thr Ala Asp Leu 
385                 390                 395                 400 


Lys Ala Leu Phe Ala Ser Ser Ala Gly Met Lys Ala Phe Thr Thr Trp 
                405                 410                 415     


Ala Ala Ala Lys Ile Ile Asp Glu Cys Arg Gln Ala Cys Gly Gly His 
            420                 425                 430         


Gly Tyr Ser Gly Tyr Asn Gly Phe Gly Gln Ala Tyr Ala Asp Trp Val 
        435                 440                 445             


Val Gln Cys Thr Trp Glu Gly Asp Asn Asn Val Leu Cys Leu Ser Met 
    450                 455                 460                 


Gly Arg Ser Leu Ile Gln Ser Cys Ile Ala Met Arg Lys Lys Lys Gly 
465                 470                 475                 480 


His Val Gly Lys Ser Val Glu Tyr Leu Gln Arg Arg Asp Glu Leu Gln 
                485                 490                 495     


Asn Ala Arg Val Asp Asn Lys Pro Leu Thr Asp Pro Ala Val Leu Ile 
            500                 505                 510         


Thr Ala Trp Glu Lys Val Ala Cys Glu Ala Ile Asn Arg Ala Thr Asp 
        515                 520                 525             


Ser Phe Ile Lys Leu Thr Gln Glu Gly Leu Ser Pro Asp Gln Ala Phe 
    530                 535                 540                 


Glu Glu Leu Ser Gln Gln Arg Phe Glu Cys Ala Arg Ile His Thr Arg 
545                 550                 555                 560 


Lys His Leu Ile Thr Ser Phe Tyr Ala Arg Ile Ser Lys Ala Lys Ala 
                565                 570                 575     


Arg Val Lys Pro His Leu Thr Val Leu Ala Asn Leu Phe Ala Val Trp 
            580                 585                 590         


Ser Ile Glu Glu Asp Ser Gly Leu Phe Leu Arg Glu Gly Cys Phe Glu 
        595                 600                 605             


Pro Ala Glu Met Asp Glu Ile Thr Ala Leu Val Asp Glu Leu Cys Cys 
    610                 615                 620                 


Glu Ala Arg Glu Gln Val Ile Gly Phe Thr Asp Ala Phe Asn Leu Ser 
625                 630                 635                 640 


Asp Phe Phe Ile Asn Ala Pro Ile Gly Arg Phe Asp Gly Asp Ala Tyr 
                645                 650                 655     


Lys His Tyr Met Asp Glu Val Lys Ala Ala Asn Asn Pro Arg Asn Thr 
            660                 665                 670         


His Ala Pro Tyr Tyr Glu Thr Lys Leu Arg Pro Phe Leu Phe Arg Pro 
        675                 680                 685             


Asp Glu Asp Glu Glu Ile Cys Asp Leu Asp Glu 
    690                 695                 


<210>  80
<211>  3278
<212>  DNA
<213>  Yarrowia lipolytica (GenBank Accession No. Z50020)


<220>
<221>  CDS
<222>  (847)..(2307)
<223>  Lip1

<400>  80
ctgcagctct tccctgccat ccgaatcatc gagaatggtc tcttcacccg atcaggcaag     60

tacaactcta ccatcaagtg gcagaagaac attttccggt tcttccttgt gtttgtcacc    120

gcctttgttg catggggagg tgccgacgat ctcgatcggt tcgtcgctct caccggatcg    180

tttgcatgtg tgcctctggt gtacatttac cctcctcttt tgcattacaa gggtgttgct    240

cgaggcacca cggctcgaac cgccgatgtc tgtattttca tctttggtct gtgtgccatg    300

ggctacacca ctgccaacac agttggttct tggattggcg gaaactagat gataccatcc    360

gtgccgcttt tcatcaagca ggtgcataga cttaaatatt tagtaaatgc acagattata    420

ttggcctgtg taagagtgta tatgtggggg atgttcgcat tatcgaggtc attgctctcc    480

atgcaacgga agtcggaacc agtcttacta cccgttgaag atgcacatct ttgcattagt    540

gtggaaaggt gcatatagcg gaatgtttcc gcccattcat aacaagtgtt ctatagatgg    600

ggtaatacag agtgagttta tgatatgatc ttgacaaggc atagaaccgc tcttctagtg    660

aagttgactt ccgacaacat ccattgctcg attcaggcga gggagactgt ggtttcagtt    720

caatcaatgc caatgtaaca tagtgtccaa agcgctttag tttagcgcgt tctggcctgt    780

gtgattatat aaggagggag atgttctgat tgttgtctac taactatcaa ttccggtcaa    840

gaaatc atg tct gtc aca tca act tct ctc aat gga acc ttc aac ggt       888
       Met Ser Val Thr Ser Thr Ser Leu Asn Gly Thr Phe Asn Gly          
       1               5                   10                           

atc tct gaa gac ggc atc gag atc ttc aag ggt atc aag tac gca aac      936
Ile Ser Glu Asp Gly Ile Glu Ile Phe Lys Gly Ile Lys Tyr Ala Asn         
15                  20                  25                  30          

aga cgg tat cga tgg gcg tat gcc gag cga atc gac gac tac gat aat      984
Arg Arg Tyr Arg Trp Ala Tyr Ala Glu Arg Ile Asp Asp Tyr Asp Asn         
                35                  40                  45              

ggc gtt ttc gac tgc act cag gaa ggc atg gct tgt ccg caa gta ctt     1032
Gly Val Phe Asp Cys Thr Gln Glu Gly Met Ala Cys Pro Gln Val Leu         
            50                  55                  60                  

cct ttc gac tac aac att gaa aag ggg ccc aag gag atg cct ttt gat     1080
Pro Phe Asp Tyr Asn Ile Glu Lys Gly Pro Lys Glu Met Pro Phe Asp         
        65                  70                  75                      

gag ttt gaa tgc tcc aat ctc atg atc act cga ccc caa ggc gct aca     1128
Glu Phe Glu Cys Ser Asn Leu Met Ile Thr Arg Pro Gln Gly Ala Thr         
    80                  85                  90                          

aat ctt cct gtg ttt gta tgg atc cac gga gga gga aat ctg gct gga     1176
Asn Leu Pro Val Phe Val Trp Ile His Gly Gly Gly Asn Leu Ala Gly         
95                  100                 105                 110         

aat ggg tac tgt tcc gat cac aac ccc gtt cct ttc gtc aag cac tcc     1224
Asn Gly Tyr Cys Ser Asp His Asn Pro Val Pro Phe Val Lys His Ser         
                115                 120                 125             

att gtg gct gga aga ccc gtt ctt cat gtg atg atc gag tac cgt ctc     1272
Ile Val Ala Gly Arg Pro Val Leu His Val Met Ile Glu Tyr Arg Leu         
            130                 135                 140                 

agt gcc ttt ggg tat ctt gct gtt ccg gac aca aat ggc aac tgg gtt     1320
Ser Ala Phe Gly Tyr Leu Ala Val Pro Asp Thr Asn Gly Asn Trp Val         
        145                 150                 155                     

ggc aat tgg ggt gcc cga gac caa tac aca gcc ttg caa tgg atc tcc     1368
Gly Asn Trp Gly Ala Arg Asp Gln Tyr Thr Ala Leu Gln Trp Ile Ser         
    160                 165                 170                         

aaa cat atc gtg gag ttt gga ggc gac cct tcc cag atc acc att gga     1416
Lys His Ile Val Glu Phe Gly Gly Asp Pro Ser Gln Ile Thr Ile Gly         
175                 180                 185                 190         

gga gag tcc gca gga agt att gga ctt cac gct ctc atg gtg cac gag     1464
Gly Glu Ser Ala Gly Ser Ile Gly Leu His Ala Leu Met Val His Glu         
                195                 200                 205             

agt atg aag ccc aag gaa gag tgt att atc cac aat gtt atc ctc tcg     1512
Ser Met Lys Pro Lys Glu Glu Cys Ile Ile His Asn Val Ile Leu Ser         
            210                 215                 220                 

tca ggg acc atg gat cga atg ggt acg ggg aca att tca gag gat gct     1560
Ser Gly Thr Met Asp Arg Met Gly Thr Gly Thr Ile Ser Glu Asp Ala         
        225                 230                 235                     

ttt aag ccc atc tat gac ggt atc aaa act ctg gtt gga gat atc aac     1608
Phe Lys Pro Ile Tyr Asp Gly Ile Lys Thr Leu Val Gly Asp Ile Asn         
    240                 245                 250                         

acc tgt tct gcg gac gag ctg ttg gag gct caa atc aaa gcc ggc ctc     1656
Thr Cys Ser Ala Asp Glu Leu Leu Glu Ala Gln Ile Lys Ala Gly Leu         
255                 260                 265                 270         

gac ctc ggc ttt tac ctc caa gac gac ttc ttc cct cca gac tgg aga     1704
Asp Leu Gly Phe Tyr Leu Gln Asp Asp Phe Phe Pro Pro Asp Trp Arg         
                275                 280                 285             

aac gtg cgg ttc aaa gtc tct cgg gtt tta ctc tcg gac gtg att gtg     1752
Asn Val Arg Phe Lys Val Ser Arg Val Leu Leu Ser Asp Val Ile Val         
            290                 295                 300                 

gat gga acc aac ttc aag aac aag atc aat cca gcg gta cga gta act     1800
Asp Gly Thr Asn Phe Lys Asn Lys Ile Asn Pro Ala Val Arg Val Thr         
        305                 310                 315                     

cca gag aac gac ttt gac cac aag gtc ttc aag ctc tac aac ata tct     1848
Pro Glu Asn Asp Phe Asp His Lys Val Phe Lys Leu Tyr Asn Ile Ser         
    320                 325                 330                         

acc gaa gat aca tgg gag gac tac cac tat aag atg atg cta ttt aag     1896
Thr Glu Asp Thr Trp Glu Asp Tyr His Tyr Lys Met Met Leu Phe Lys         
335                 340                 345                 350         

gga gac gag acc ttt att cgt gga aac cag cag ctg gaa ctt ctg ttt     1944
Gly Asp Glu Thr Phe Ile Arg Gly Asn Gln Gln Leu Glu Leu Leu Phe         
                355                 360                 365             

gag cag gaa aat atc ccc gtc tgg aga cag ttg ttc gac cag att cat     1992
Glu Gln Glu Asn Ile Pro Val Trp Arg Gln Leu Phe Asp Gln Ile His         
            370                 375                 380                 

cca aat gac ccc agt cgt ctc tgc cac cat gcc gtg gat ctc tat tac     2040
Pro Asn Asp Pro Ser Arg Leu Cys His His Ala Val Asp Leu Tyr Tyr         
        385                 390                 395                     

atg tgg gac aac tgg gag atg cct gaa gat aaa cat gca gtg gcc cgt     2088
Met Trp Asp Asn Trp Glu Met Pro Glu Asp Lys His Ala Val Ala Arg         
    400                 405                 410                         

cag tac cag gac act ctg acc aag ttt gtt tac gga caa gac ccc tgg     2136
Gln Tyr Gln Asp Thr Leu Thr Lys Phe Val Tyr Gly Gln Asp Pro Trp         
415                 420                 425                 430         

ccg gtc gac aag ctc cat tat gtc cac gat aac cag ttt gag atc cta     2184
Pro Val Asp Lys Leu His Tyr Val His Asp Asn Gln Phe Glu Ile Leu         
                435                 440                 445             

gac aag tct cag ttt ggc gac ttc cgc aac gtt cca gcc ctc aag ttt     2232
Asp Lys Ser Gln Phe Gly Asp Phe Arg Asn Val Pro Ala Leu Lys Phe         
            450                 455                 460                 

ctc ttg gga ttc tca gcc gaa gag ctt gga gaa ctc acg aaa aag tac     2280
Leu Leu Gly Phe Ser Ala Glu Glu Leu Gly Glu Leu Thr Lys Lys Tyr         
        465                 470                 475                     

act gga gag ggt cac tac acg ttg tag ggttcatgag aagataaata           2327
Thr Gly Glu Gly His Tyr Thr Leu                                         
    480                 485                                             

tataaataca ttgagatatt aaatgcgcta gattagagag cctcatactg ctcggagaga   2387

agccaagacg agtactcaaa ggggattaca ccatccatat ccacagacac aagctgggga   2447

aaggttctat atacactttc cggaataccg tagtttccga tgttatcaat gggggcagcc   2507

aggatttcag gcacttcggt gtctcggggt gaaatggcgt tcttggcctc catcaagtcg   2567

taccatgtct tcatttgcct gtcaaagtaa aacagaagca gatgaagaat gaacttgaag   2627

tgaaggacgt gactacaaca gcctgcctgg tcaaccacat cattcatgtt gctgatgatc   2687

ttctgaatca gctgaagagg gtactctgtc tcaatcagat caccaaattt ggggtgcagt   2747

agggcacatg atcgaagcat gagtgcagta gtagcacaat aagtcattcg agcaaggtag   2807

agagtactcg acgacagaat ctgcattgtg tagagctccg agaatccctc cagggatgtt   2867

cgcaccgcat cggcagtggc atggcactat tgatcactgc gggggtcagt ttgagatcga   2927

agatgttcag accaaactcg gagaagggcg ttcgaggaac agtgggtgac acgttcgtca   2987

acagagatag ttcatgcaga gagatcttga gagaagattt gaacaactcg agaacgtgat   3047

caattgggaa ccctcgcttg tcaaaggtga acacctcgtc gattcgacgg tcgaaatgct   3107

tgaccagccg ctgagtacgt tcatcattga tatccagagg cttcttgtaa tcctcacttc   3167

taagcatggc catcatatct tccaactcct tgaacacttt ggccagcttg gcacatctga   3227

tctcattaat ggttgaatca ggtgtggaaa agaggtcaca gcattctgca g            3278


<210>  81
<211>  486
<212>  PRT
<213>  Yarrowia lipolytica (GenBank Accession No. Z50020)

<400>  81

Met Ser Val Thr Ser Thr Ser Leu Asn Gly Thr Phe Asn Gly Ile Ser 
1               5                   10                  15      


Glu Asp Gly Ile Glu Ile Phe Lys Gly Ile Lys Tyr Ala Asn Arg Arg 
            20                  25                  30          


Tyr Arg Trp Ala Tyr Ala Glu Arg Ile Asp Asp Tyr Asp Asn Gly Val 
        35                  40                  45              


Phe Asp Cys Thr Gln Glu Gly Met Ala Cys Pro Gln Val Leu Pro Phe 
    50                  55                  60                  


Asp Tyr Asn Ile Glu Lys Gly Pro Lys Glu Met Pro Phe Asp Glu Phe 
65                  70                  75                  80  


Glu Cys Ser Asn Leu Met Ile Thr Arg Pro Gln Gly Ala Thr Asn Leu 
                85                  90                  95      


Pro Val Phe Val Trp Ile His Gly Gly Gly Asn Leu Ala Gly Asn Gly 
            100                 105                 110         


Tyr Cys Ser Asp His Asn Pro Val Pro Phe Val Lys His Ser Ile Val 
        115                 120                 125             


Ala Gly Arg Pro Val Leu His Val Met Ile Glu Tyr Arg Leu Ser Ala 
    130                 135                 140                 


Phe Gly Tyr Leu Ala Val Pro Asp Thr Asn Gly Asn Trp Val Gly Asn 
145                 150                 155                 160 


Trp Gly Ala Arg Asp Gln Tyr Thr Ala Leu Gln Trp Ile Ser Lys His 
                165                 170                 175     


Ile Val Glu Phe Gly Gly Asp Pro Ser Gln Ile Thr Ile Gly Gly Glu 
            180                 185                 190         


Ser Ala Gly Ser Ile Gly Leu His Ala Leu Met Val His Glu Ser Met 
        195                 200                 205             


Lys Pro Lys Glu Glu Cys Ile Ile His Asn Val Ile Leu Ser Ser Gly 
    210                 215                 220                 


Thr Met Asp Arg Met Gly Thr Gly Thr Ile Ser Glu Asp Ala Phe Lys 
225                 230                 235                 240 


Pro Ile Tyr Asp Gly Ile Lys Thr Leu Val Gly Asp Ile Asn Thr Cys 
                245                 250                 255     


Ser Ala Asp Glu Leu Leu Glu Ala Gln Ile Lys Ala Gly Leu Asp Leu 
            260                 265                 270         


Gly Phe Tyr Leu Gln Asp Asp Phe Phe Pro Pro Asp Trp Arg Asn Val 
        275                 280                 285             


Arg Phe Lys Val Ser Arg Val Leu Leu Ser Asp Val Ile Val Asp Gly 
    290                 295                 300                 


Thr Asn Phe Lys Asn Lys Ile Asn Pro Ala Val Arg Val Thr Pro Glu 
305                 310                 315                 320 


Asn Asp Phe Asp His Lys Val Phe Lys Leu Tyr Asn Ile Ser Thr Glu 
                325                 330                 335     


Asp Thr Trp Glu Asp Tyr His Tyr Lys Met Met Leu Phe Lys Gly Asp 
            340                 345                 350         


Glu Thr Phe Ile Arg Gly Asn Gln Gln Leu Glu Leu Leu Phe Glu Gln 
        355                 360                 365             


Glu Asn Ile Pro Val Trp Arg Gln Leu Phe Asp Gln Ile His Pro Asn 
    370                 375                 380                 


Asp Pro Ser Arg Leu Cys His His Ala Val Asp Leu Tyr Tyr Met Trp 
385                 390                 395                 400 


Asp Asn Trp Glu Met Pro Glu Asp Lys His Ala Val Ala Arg Gln Tyr 
                405                 410                 415     


Gln Asp Thr Leu Thr Lys Phe Val Tyr Gly Gln Asp Pro Trp Pro Val 
            420                 425                 430         


Asp Lys Leu His Tyr Val His Asp Asn Gln Phe Glu Ile Leu Asp Lys 
        435                 440                 445             


Ser Gln Phe Gly Asp Phe Arg Asn Val Pro Ala Leu Lys Phe Leu Leu 
    450                 455                 460                 


Gly Phe Ser Ala Glu Glu Leu Gly Glu Leu Thr Lys Lys Tyr Thr Gly 
465                 470                 475                 480 


Glu Gly His Tyr Thr Leu 
                485     


<210>  82
<211>  5304
<212>  DNA
<213>  Yarrowia lipolytica (GenBank Accession No. AJ012632)


<220>
<221>  CDS
<222>  (3737)..(4741)
<223>  Lip2

<400>  82
gatatcggta gcaaacagac acgaagaaga ggccttggag aatttggaaa caacatccat     60

tcgtgctcct tgtttctgct tgccatgcaa atgcaacagg gggataccgg gctgcagagt    120

tcggaaggtc tcgtagacgt atcgcacctg ttttgatgaa ctgaagaaaa cgatgatctt    180

gaacttggtg tgtgttctca ggaaacccca cagtgtgtcc agcttgtctt gcagctccac    240

gcagacatag ttctgttcga ggttcttggg tgtgctggaa gtggtgtccg ggttggcgga    300

gatgtacttg gggtcggcga gtgacaaacg ggccagatca ctgacgctct tggtctgtgt    360

tgcgctgaaa agcagagtct gtctgtccac aggcaggttt tccaaaatgg catccatggt    420

cttcttgaaa cccatatcga ggattcgatc ggcctcgtcc agcaccagca tcttcacgtt    480

agaaagatca aacccgctag tttggtccat gtgttgtaac agtcgtccgg gtgtacaaat    540

gagaatgttc agcttggcca gtcggtcagc ctccatggca acgtcctttc ctccaataac    600

caaaccagca gagaagctgt gacaacggcc gatttttcgc agaacctgga aaatctggac    660

cgccagttct cgagtcggac taataaccag ggctcctaaa ccatccacat cactccactt    720

gtttcgaaac aggcactcca acacaggcac cagaaacgcc agagtcttac cacttccggt    780

acgcgctgct ccaagcagat cgtggccttg cagagcggga gggatggccc gcttctgaac    840

atcggtacag gtgacataat gtgaattttt tagaccctca atagtagcct cagacaaggg    900

cagctcctca aacttggaca cctccttgtc ggcctcgtcc tctccaaagt tgtcgacgcg    960

ctcctgcagc ttggagagct tctgtttgtg ttcctctcgt ttgattttac gaggatcggc   1020

tcgtttcttc attttcgcca tatctcaagc tgtcggataa atttcttgga caaaaaaatc   1080

taacttttcg tgcgccttca cttttatcag tctgaaaatt acacatgcat gcagcttgga   1140

gtatggaggc gacagaggtg catgccagag tgcatgaatt agggcaacgg gggtgtgaag   1200

aagggcgcaa aagatgtgac gacggtgcaa gacgacggta tactgcagac aaggtctttc   1260

actgcaaaac tcctggttaa atcgtgggtc ccgcgtctcc gtatcatatg actttgggtc   1320

acgtgactgc aagattttta aggtttcgaa ctcccaaatt gagtcacgtg tcgtctctct   1380

gcaacatgag gtatcgtaaa ttaggaatga ctcgacctga caggccatca aggaccctga   1440

tgcaactctg aaattagagg gaggaggtaa tgagaaacag tgctggattg tggtgagaca   1500

ttgaagcggg attggttaac ctgtttcttt tttgtcgttt gctcttcatt attgcgattt   1560

ttgtagtcat ttgattagat cagatcagca gtcccctcat gctgtcgttg atgggcgcgt   1620

aatcagtgta acgctttgaa tgaggatggt gtgagtaata ctggtttcat tggggggggg   1680

ggggggggta ctgttactcg aaaaacaaat catatgcatc tctgaacgtg ccggggtgtg   1740

gcatgtttct cgcttcgaaa aataaatgtt ggctgttcca ggcttgtctc agacctgcaa   1800

gaatcatact ggcgtaatct ccgccaggtc tggtcaggaa caagagagta aaagaaatgt   1860

ttgagtctcg gctttcacat cctcggcact agataagcag caatatcaat gtcacattgc   1920

ttgcttgtgc aggtcagggg gagggggatg tatatgatac aagcaggttc gcatctacag   1980

aatctacagt atgtatctga tgatagcctt acacagcgca cgcagatgta gtttttgggt   2040

tgaacagtgt cagcggaagc ggcgtagtgg ggtactgtag tacagaatca acggaatgaa   2100

gtttacaact gcttctgtta ctgtagcctt tcactgttca aatgataacg ttggagaaga   2160

gtgcaccctc tgttagatag gattacagtc cagacacaca ttggcaccga agtctgtagt   2220

caaacattgg tctcagttgg tgtaatgaca ttcaatagac tgattctgca actcctacac   2280

cttgcattgt acacccgaga ccccttgtac ggcccgcatt tctccttcac atgtcggcat   2340

gtgtcttgtc tgtagccact tcaatgggat gcagtattag cactgtcgga cacacctcag   2400

tcaatgggaa tggaatgcca tgctcctagc ggctcattgt ttgctaggaa atacagttaa   2460

tagcacagat catagagtct aggctctata cgtcttagaa caaccattat ttgtttaaac   2520

gacaacttgg taagcgaatt acagtaagta gctattgttt ctatctctgt cagtgttcgg   2580

ataagtcctt agatcaccat actcaaagtg gtacgtttcg tcgcatcgga cgagaagtgt   2640

gtctatcacc gtttcttttt gccgctctca tttttgcggt cggaataatt actgtggacc   2700

ctcggcgccg accgttttgc agggctcatc aacctaaaac ttctccgagt ctgtgccttc   2760

aggtgggcat agttgatggg tgttttgaag ttaatagtgg ggaagaacta tggcaaacaa   2820

gcagatgcag gcaccttgta actgcagacc ggttcttgtc taccgactcc gctgcacctg   2880

tgccgcggta catgtcgtca caggctgcgg ggttcggagg cccccttgca acctcctttg   2940

atagttgcta tggccccaaa gagttatacg agatagaccc acagatctac ttgactgttg   3000

tcacagaacc tgctaggttt gcttattgta cccgctttgt agctactgta caacgacaac   3060

gtctaaaatt gagacgcgaa caaactccag atgcagaacc caaacctctc tctcagagtt   3120

tcgagtgctt ctacctcaca gtaaagtgga ggtggacctg caagggaatt cagtcacaag   3180

gccccgaatg tctccgaaac tccaatcgga ccgtttaaac agactaatat cacgtcattg   3240

attgatatta gcatccggca agagccgcaa ggttatctcc tcaccaatga gcctgttgta   3300

cggctcattc cgcatctgcg gctgattcag tttcgagtgg ggatggtaga cttcattgca   3360

gcattcctaa ccttctactt ggtccgtgga gatgtcatgg acatcgattt tgggctgaga   3420

agccttttga cgatgtcgat atcactgacc gctaatttac tctggcagtt tctccggctc   3480

tcgaggcatc gtcgatcacc aaacactatc tgctagtcta aatgtccgac acgacagctt   3540

ttgatcgccg tgaacggcgc agacctcatg caccatgcac cagggccaaa tcaattacgg   3600

gtcgcttagc gttgcagtcg gggcattatg gtggaagttc cgatacggca cagacacatt   3660

ccatagtggg gggattggat tataaaaggg ccatagaaag ccctcaattg atacccaagt   3720

accagctccc ctcact atg aag ctt tcc acc atc ctt ttc aca gcc tgc gct   3772
                  Met Lys Leu Ser Thr Ile Leu Phe Thr Ala Cys Ala       
                  1               5                   10                

acc ctg gct gcc gcc ctc cct tcc ccc atc act cct tct gag gcc gca     3820
Thr Leu Ala Ala Ala Leu Pro Ser Pro Ile Thr Pro Ser Glu Ala Ala         
        15                  20                  25                      

gtt ctc cag aag cga gtg tac acc tct acc gag acc tct cac att gac     3868
Val Leu Gln Lys Arg Val Tyr Thr Ser Thr Glu Thr Ser His Ile Asp         
    30                  35                  40                          

cag gag tcc tac aac ttc ttt gag aag tac gcc cga ctc gca aac att     3916
Gln Glu Ser Tyr Asn Phe Phe Glu Lys Tyr Ala Arg Leu Ala Asn Ile         
45                  50                  55                  60          

gga tat tgt gtt ggt ccc ggc act aag atc ttc aag ccc ttc aac tgt     3964
Gly Tyr Cys Val Gly Pro Gly Thr Lys Ile Phe Lys Pro Phe Asn Cys         
                65                  70                  75              

ggc ctg caa tgt gcc cac ttc ccc aac gtt gag ctc atc gag gag ttc     4012
Gly Leu Gln Cys Ala His Phe Pro Asn Val Glu Leu Ile Glu Glu Phe         
            80                  85                  90                  

cac gac ccc cgt ctc atc ttt gat gtt tct ggt tac ctc gct gtt gat     4060
His Asp Pro Arg Leu Ile Phe Asp Val Ser Gly Tyr Leu Ala Val Asp         
        95                  100                 105                     

cat gcc tcc aag cag atc tac ctt gtt att cga gga acc cac tct ctg     4108
His Ala Ser Lys Gln Ile Tyr Leu Val Ile Arg Gly Thr His Ser Leu         
    110                 115                 120                         

gag gac gtc ata acc gac atc cga atc atg cag gct cct ctg acg aac     4156
Glu Asp Val Ile Thr Asp Ile Arg Ile Met Gln Ala Pro Leu Thr Asn         
125                 130                 135                 140         

ttt gat ctt gct gct aac atc tct tct act gct act tgt gat gac tgt     4204
Phe Asp Leu Ala Ala Asn Ile Ser Ser Thr Ala Thr Cys Asp Asp Cys         
                145                 150                 155             

ctt gtc cac aat ggc ttc atc cag tcc tac aac aac acc tac aat cag     4252
Leu Val His Asn Gly Phe Ile Gln Ser Tyr Asn Asn Thr Tyr Asn Gln         
            160                 165                 170                 

atc ggc ccc aag ctc gac tct gtg att gag cag tat ccc gac tac cag     4300
Ile Gly Pro Lys Leu Asp Ser Val Ile Glu Gln Tyr Pro Asp Tyr Gln         
        175                 180                 185                     

att gct gtc acc ggt cac tct ctc gga gga gct gca gcc ctt ctg ttc     4348
Ile Ala Val Thr Gly His Ser Leu Gly Gly Ala Ala Ala Leu Leu Phe         
    190                 195                 200                         

gga atc aac ctc aag gtt aac ggc cac gat ccc ctc gtt gtt act ctt     4396
Gly Ile Asn Leu Lys Val Asn Gly His Asp Pro Leu Val Val Thr Leu         
205                 210                 215                 220         

ggt cag ccc att gtc ggt aac gct ggc ttt gct aac tgg gtc gat aaa     4444
Gly Gln Pro Ile Val Gly Asn Ala Gly Phe Ala Asn Trp Val Asp Lys         
                225                 230                 235             

ctc ttc ttt ggc cag gag aac ccc gat gtc tcc aag gtg tcc aaa gac     4492
Leu Phe Phe Gly Gln Glu Asn Pro Asp Val Ser Lys Val Ser Lys Asp         
            240                 245                 250                 

cga aag ctc tac cga atc acc cac cga gga gat atc gtc cct caa gtg     4540
Arg Lys Leu Tyr Arg Ile Thr His Arg Gly Asp Ile Val Pro Gln Val         
        255                 260                 265                     

ccc ttc tgg gac ggt tac cag cac tgc tct ggt gag gtc ttt att gac     4588
Pro Phe Trp Asp Gly Tyr Gln His Cys Ser Gly Glu Val Phe Ile Asp         
    270                 275                 280                         

tgg ccc ctg atc cac cct cct ctc tcc aac gtt gtc atg tgc cag ggc     4636
Trp Pro Leu Ile His Pro Pro Leu Ser Asn Val Val Met Cys Gln Gly         
285                 290                 295                 300         

cag agc aat aaa cag tgc tct gcc ggt aac act ctg ctc cag cag gtc     4684
Gln Ser Asn Lys Gln Cys Ser Ala Gly Asn Thr Leu Leu Gln Gln Val         
                305                 310                 315             

aat gtg att gga aac cat ctg cag tac ttc gtc acc gag ggt gtc tgt     4732
Asn Val Ile Gly Asn His Leu Gln Tyr Phe Val Thr Glu Gly Val Cys         
            320                 325                 330                 

ggt atc taa gctatttatc actctttaca acttctacct caactatcta             4781
Gly Ile                                                                 
                                                                        

ctttaataaa tgaatatcgt ttattctcta tgattactgt atatgcgttc ctctaagaca   4841

aatcgaaacc agcatgcgat cgaatggcat acaaaagttt cttccgaagt tgatcaatgt   4901

cctgatagtc aggcagcttg agaagattga cacaggtgga ggccgtaggg aaccgatcaa   4961

cctgtctacc agcgttacga atggcaaatg acgggttcaa agccttgaat ccttgcaatg   5021

gtgccttgga tactgatgtc acaaacttaa gaagcagccg cttgtcctct tcctcgaaac   5081

tctcaaacac agtccagagg tcctttatag cttgatctgt atccagatag cctccgtaat   5141

tggtgtgtgt cttcaaatcc cagacgtcca cattggcatg tcctccactg ataagcattt   5201

gaagttcatc tgcgttgaac attgagaccc acgaagggtc aatgagctgg tatagaccgc   5261

ccaagaatgc atctgtctgt gttctgatac tggtgttaag ctt                     5304


<210>  83
<211>  334
<212>  PRT
<213>  Yarrowia lipolytica (GenBank Accession No. AJ012632)

<400>  83

Met Lys Leu Ser Thr Ile Leu Phe Thr Ala Cys Ala Thr Leu Ala Ala 
1               5                   10                  15      


Ala Leu Pro Ser Pro Ile Thr Pro Ser Glu Ala Ala Val Leu Gln Lys 
            20                  25                  30          


Arg Val Tyr Thr Ser Thr Glu Thr Ser His Ile Asp Gln Glu Ser Tyr 
        35                  40                  45              


Asn Phe Phe Glu Lys Tyr Ala Arg Leu Ala Asn Ile Gly Tyr Cys Val 
    50                  55                  60                  


Gly Pro Gly Thr Lys Ile Phe Lys Pro Phe Asn Cys Gly Leu Gln Cys 
65                  70                  75                  80  


Ala His Phe Pro Asn Val Glu Leu Ile Glu Glu Phe His Asp Pro Arg 
                85                  90                  95      


Leu Ile Phe Asp Val Ser Gly Tyr Leu Ala Val Asp His Ala Ser Lys 
            100                 105                 110         


Gln Ile Tyr Leu Val Ile Arg Gly Thr His Ser Leu Glu Asp Val Ile 
        115                 120                 125             


Thr Asp Ile Arg Ile Met Gln Ala Pro Leu Thr Asn Phe Asp Leu Ala 
    130                 135                 140                 


Ala Asn Ile Ser Ser Thr Ala Thr Cys Asp Asp Cys Leu Val His Asn 
145                 150                 155                 160 


Gly Phe Ile Gln Ser Tyr Asn Asn Thr Tyr Asn Gln Ile Gly Pro Lys 
                165                 170                 175     


Leu Asp Ser Val Ile Glu Gln Tyr Pro Asp Tyr Gln Ile Ala Val Thr 
            180                 185                 190         


Gly His Ser Leu Gly Gly Ala Ala Ala Leu Leu Phe Gly Ile Asn Leu 
        195                 200                 205             


Lys Val Asn Gly His Asp Pro Leu Val Val Thr Leu Gly Gln Pro Ile 
    210                 215                 220                 


Val Gly Asn Ala Gly Phe Ala Asn Trp Val Asp Lys Leu Phe Phe Gly 
225                 230                 235                 240 


Gln Glu Asn Pro Asp Val Ser Lys Val Ser Lys Asp Arg Lys Leu Tyr 
                245                 250                 255     


Arg Ile Thr His Arg Gly Asp Ile Val Pro Gln Val Pro Phe Trp Asp 
            260                 265                 270         


Gly Tyr Gln His Cys Ser Gly Glu Val Phe Ile Asp Trp Pro Leu Ile 
        275                 280                 285             


His Pro Pro Leu Ser Asn Val Val Met Cys Gln Gly Gln Ser Asn Lys 
    290                 295                 300                 


Gln Cys Ser Ala Gly Asn Thr Leu Leu Gln Gln Val Asn Val Ile Gly 
305                 310                 315                 320 


Asn His Leu Gln Tyr Phe Val Thr Glu Gly Val Cys Gly Ile 
                325                 330                 


<210>  84
<211>  3630
<212>  DNA
<213>  Yarrowia lipolytica (GenBank Accession No. AJ249751)


<220>
<221>  CDS
<222>  (1810)..(3306)
<223>  Lip3

<400>  84
cgacactgaa acagactccc gtcttcgtct gcgatacatg gcttcgagag tcgcatttcc     60

acactcagcg gtagatgcac ctgcgacggc aaggcttcag tccgactcac attcccgcgt    120

ctgatctccc agagaactcc ggagtggtct acacaatgtt atcgataccc ccggcaaatg    180

actccattct gcccacaaaa ctttgacaaa ttccaccccc agtccccgaa taagatatca    240

cgggactcaa gcaatcgctc tctagcatgt ttcagttctt tgaattcata ttctgactat    300

cgcccaaaaa gtcacgttac ggaccaaacg aaatcacatg acccatgaaa cgaaatcaca    360

tgaccagcag ccatttagat cgtcgttttg gtcacatgac atatcgcttt cacccctccg    420

aaatgtctcc ggaacctgcc tctagtaccc taaccctgtt cagatgcatc ggttttaacg    480

gtttttcggc cgactttatt tgtgtggggt aaggatgagc cgaggttgtg aattgcggtt    540

taggatcaag tatttcaggg ttaggcaagt aaatggagtg ggcaaagggg ttggggttga    600

tatgaggaaa aaaattggtc tccacaagtc tccgttgttc cagcagagat cgagtctgta    660

tttttcacat ttgtttctaa ttttgattac taacaaacat taaatatatc ggttatagaa    720

atatacataa atggcgtcat tatgagtgag ttgtagttga tatatacaag aaaatcggcg    780

aaaaaagtca atatgaaaac gagcaagaac tccccgacgt aatctgatca ttattggacg    840

agttacaggg agtaggatgt ggttgaggtc tatggttgga gtaattgtag tatggaagaa    900

gttacagagg ttgaagagcc tccgctggaa cttggaatgt gttaattggg gtggtatatt    960

tctactgaag taaattcatc aaaatccgat gggaattggg gtgattgccg atatgcgatt   1020

ttcgacgcat gtcttgtggt ctttgctatc gcttgatagg aagacgataa agactggaaa   1080

taggtccaat gacacattat actacgagaa aattggattg agaccaataa cgggcacttt   1140

tgagtattct ttattccatg agaggagatt tacatgttgg aaacagaaca ggggttttaa   1200

gagtctaagt acttcagaat cgtcattttt gacgctgaaa actgtgaaaa aaacgttagc   1260

tataattgct gccgttgtgg agacatatct gatcaacttc aaaccccaga atgtctctca   1320

tttacgtcat ctgaatcgga tctcattttt ctccatgaaa tctccaactt taatgattag   1380

ccgagagatt gcggtcagac ggacgcaagt tgccgaaaaa cggtcctata gcggatatta   1440

aagtgggatt caattctcct agttccaagg agtccactga acctctctca tctcctacat   1500

tttcccccaa aacccttcaa aatggagcat ctatttcacc cccgattcca catgcacccc   1560

acacgcatgc atgcatgcag ctgcataaac cgaaaaacgc accgaaataa aagatagaga   1620

aagaagttga cttcctctcc attttttgtg acgagttaaa caaataccac gcgcgcgaaa   1680

acggcccaga aacaactcga atcgacttgc aatccgccca gcgagtctca cagacgcaaa   1740

ccaatagaca caccaatcga caccacacca aacgacacta ccccaataga cggtctttcc   1800

ccagtcgcc atg cct ctc gaa ctc ccc tcg ctc aac gcc tcg att gtc ggc   1851
          Met Pro Leu Glu Leu Pro Ser Leu Asn Ala Ser Ile Val Gly       
          1               5                   10                        

aac acc gtt cag aac ggc gct gtt gag cag ttt ctc aac atc cga tac     1899
Asn Thr Val Gln Asn Gly Ala Val Glu Gln Phe Leu Asn Ile Arg Tyr         
15                  20                  25                  30          

gcc gac att cct ggc aag ttt gag aag ccc gtg ctc aag aac gat tgg     1947
Ala Asp Ile Pro Gly Lys Phe Glu Lys Pro Val Leu Lys Asn Asp Trp         
                35                  40                  45              

aac ggc gcg gag atc gac gcc acc aag gtc ggt ccc gtg tgc ccc caa     1995
Asn Gly Ala Glu Ile Asp Ala Thr Lys Val Gly Pro Val Cys Pro Gln         
            50                  55                  60                  

ccc cgc acc cca ttc aac ttc ttc tcc gtg cca gac gac ctc tgg gag     2043
Pro Arg Thr Pro Phe Asn Phe Phe Ser Val Pro Asp Asp Leu Trp Glu         
        65                  70                  75                      

aaa gtc aat gtg gac acg tac cag gac ggt ctg ctg tgc gac aac ctg     2091
Lys Val Asn Val Asp Thr Tyr Gln Asp Gly Leu Leu Cys Asp Asn Leu         
    80                  85                  90                          

att gtg acg cga ccg aag ggc gtg tct gcc aac gcc cgg ctg ccc act     2139
Ile Val Thr Arg Pro Lys Gly Val Ser Ala Asn Ala Arg Leu Pro Thr         
95                  100                 105                 110         

gtt gtg tgg atc cac ggc ggc tcc aat att gag ggc agt atc tac aac     2187
Val Val Trp Ile His Gly Gly Ser Asn Ile Glu Gly Ser Ile Tyr Asn         
                115                 120                 125             

ctc atc tat gag ccc cag ttc ctg gtg gca gag tcg gtg cga gta ggc     2235
Leu Ile Tyr Glu Pro Gln Phe Leu Val Ala Glu Ser Val Arg Val Gly         
            130                 135                 140                 

aag ccg att gtg cac gtg tgt atc gag tac cga ctg ggt ctc gcg ggc     2283
Lys Pro Ile Val His Val Cys Ile Glu Tyr Arg Leu Gly Leu Ala Gly         
        145                 150                 155                     

ttc ctc acc aag aac ggc aag ggc aac tgg ggc acg tgg gat cag tac     2331
Phe Leu Thr Lys Asn Gly Lys Gly Asn Trp Gly Thr Trp Asp Gln Tyr         
    160                 165                 170                         

acg ggc tgc cag tgg gtc aac cgc cac att cag gac ttt gga ggc gat     2379
Thr Gly Cys Gln Trp Val Asn Arg His Ile Gln Asp Phe Gly Gly Asp         
175                 180                 185                 190         

cct ttg aac gtg aca ttg acc ggt gag tct gcc ggc tct gta gca gtc     2427
Pro Leu Asn Val Thr Leu Thr Gly Glu Ser Ala Gly Ser Val Ala Val         
                195                 200                 205             

cat aac atg ctc atc aag gac tcc atg aac ggc cga aag ttg ttc cga     2475
His Asn Met Leu Ile Lys Asp Ser Met Asn Gly Arg Lys Leu Phe Arg         
            210                 215                 220                 

aat gcc gtc atg atg agt ggc act ctc gag acc atc act cct cag cct     2523
Asn Ala Val Met Met Ser Gly Thr Leu Glu Thr Ile Thr Pro Gln Pro         
        225                 230                 235                     

ccc aag tgg cat gct cgt ttg gag gag aag gtg gcc aag gtc act ggc     2571
Pro Lys Trp His Ala Arg Leu Glu Glu Lys Val Ala Lys Val Thr Gly         
    240                 245                 250                         

aag gaa gtg gcc gac ctt gct tct ctg tcc gat aag gag ctg ctc gac     2619
Lys Glu Val Ala Asp Leu Ala Ser Leu Ser Asp Lys Glu Leu Leu Asp         
255                 260                 265                 270         

gcc cag atc aag ctc aat gtg gct gtg tgc atg act tgc gac gac ggc     2667
Ala Gln Ile Lys Leu Asn Val Ala Val Cys Met Thr Cys Asp Asp Gly         
                275                 280                 285             

gac ttt ttc gag ccc gga tgg aag cag cat ctg act cct gac tgg ctc     2715
Asp Phe Phe Glu Pro Gly Trp Lys Gln His Leu Thr Pro Asp Trp Leu         
            290                 295                 300                 

gac aag ctc atc atc tcc gat tgc aag gac gag ggc atg ctg tat ttc     2763
Asp Lys Leu Ile Ile Ser Asp Cys Lys Asp Glu Gly Met Leu Tyr Phe         
        305                 310                 315                     

ctg cca gtc aac gcg cag gac gac gag gag ctg ttg gca aag gtg gcc     2811
Leu Pro Val Asn Ala Gln Asp Asp Glu Glu Leu Leu Ala Lys Val Ala         
    320                 325                 330                         

aag tcg ccc gtg ggt aag gag att tcc gag ctt tac ggc atc aag gag     2859
Lys Ser Pro Val Gly Lys Glu Ile Ser Glu Leu Tyr Gly Ile Lys Glu         
335                 340                 345                 350         

ggt ggc gat atc aag tct gcg tgt ctc gat ctc aag act gac gcc acc     2907
Gly Gly Asp Ile Lys Ser Ala Cys Leu Asp Leu Lys Thr Asp Ala Thr         
                355                 360                 365             

ttc aat tac ttt aac cat ctg ctg ttc aag aag atg gag gag gcc cga     2955
Phe Asn Tyr Phe Asn His Leu Leu Phe Lys Lys Met Glu Glu Ala Arg         
            370                 375                 380                 

aac aac ggc tcc act tct cga gtt tac cgt ctg gcc gtc gat gag ccc     3003
Asn Asn Gly Ser Thr Ser Arg Val Tyr Arg Leu Ala Val Asp Glu Pro         
        385                 390                 395                     

aac ccc cac aac ccc gac cag cgg gcc cac cac gcc gtc gac gtg ctg     3051
Asn Pro His Asn Pro Asp Gln Arg Ala His His Ala Val Asp Val Leu         
    400                 405                 410                         

tac atg ttc aac tcg acc aag ttc aac gag cac ggc gac aag ctg tct     3099
Tyr Met Phe Asn Ser Thr Lys Phe Asn Glu His Gly Asp Lys Leu Ser         
415                 420                 425                 430         

cgg ctg ttc cag agc cac ttt ttg cgg ctg gcg tat ggc ctg gag ccc     3147
Arg Leu Phe Gln Ser His Phe Leu Arg Leu Ala Tyr Gly Leu Glu Pro         
                435                 440                 445             

tgg gac cat cga aac ttt gga gtg tac aga aac ggc ggc tac cag cag     3195
Trp Asp His Arg Asn Phe Gly Val Tyr Arg Asn Gly Gly Tyr Gln Gln         
            450                 455                 460                 

ctg ccg ctg agt gag ttg aac aag gtc cga ccc gtc gag cgg tac gag     3243
Leu Pro Leu Ser Glu Leu Asn Lys Val Arg Pro Val Glu Arg Tyr Glu         
        465                 470                 475                     

gcg ctg tcc aag atg gac ttt ggc cag gtt ggg cgt ttg tcc aat gcg     3291
Ala Leu Ser Lys Met Asp Phe Gly Gln Val Gly Arg Leu Ser Asn Ala         
    480                 485                 490                         

ctt tcg cgc cta tga atcgcggatg aatatctgga cacgtatcac tatgaaattg     3346
Leu Ser Arg Leu                                                         
495                                                                     

ccattgatga aatcgggctt agtgaatgtg agccagggga tgagatggta gatgttgatg   3406

tgcgttcaag tgattcggtt gatgttggag gacaggccga gccgcgaaga tagatagatt   3466

tgtaaatgag tgttttgttg atcttatcga atatctccca ttctctactg tacgtactgc   3526

agcgcaatgt agttgctgga ggtggttggg cgacttgttg agcatcagga ccagctgatc   3586

gggatataca atacatggtt gtggtcggct tgacggcagt tttc                    3630


<210>  85
<211>  498
<212>  PRT
<213>  Yarrowia lipolytica (GenBank Accession No. AJ249751)

<400>  85

Met Pro Leu Glu Leu Pro Ser Leu Asn Ala Ser Ile Val Gly Asn Thr 
1               5                   10                  15      


Val Gln Asn Gly Ala Val Glu Gln Phe Leu Asn Ile Arg Tyr Ala Asp 
            20                  25                  30          


Ile Pro Gly Lys Phe Glu Lys Pro Val Leu Lys Asn Asp Trp Asn Gly 
        35                  40                  45              


Ala Glu Ile Asp Ala Thr Lys Val Gly Pro Val Cys Pro Gln Pro Arg 
    50                  55                  60                  


Thr Pro Phe Asn Phe Phe Ser Val Pro Asp Asp Leu Trp Glu Lys Val 
65                  70                  75                  80  


Asn Val Asp Thr Tyr Gln Asp Gly Leu Leu Cys Asp Asn Leu Ile Val 
                85                  90                  95      


Thr Arg Pro Lys Gly Val Ser Ala Asn Ala Arg Leu Pro Thr Val Val 
            100                 105                 110         


Trp Ile His Gly Gly Ser Asn Ile Glu Gly Ser Ile Tyr Asn Leu Ile 
        115                 120                 125             


Tyr Glu Pro Gln Phe Leu Val Ala Glu Ser Val Arg Val Gly Lys Pro 
    130                 135                 140                 


Ile Val His Val Cys Ile Glu Tyr Arg Leu Gly Leu Ala Gly Phe Leu 
145                 150                 155                 160 


Thr Lys Asn Gly Lys Gly Asn Trp Gly Thr Trp Asp Gln Tyr Thr Gly 
                165                 170                 175     


Cys Gln Trp Val Asn Arg His Ile Gln Asp Phe Gly Gly Asp Pro Leu 
            180                 185                 190         


Asn Val Thr Leu Thr Gly Glu Ser Ala Gly Ser Val Ala Val His Asn 
        195                 200                 205             


Met Leu Ile Lys Asp Ser Met Asn Gly Arg Lys Leu Phe Arg Asn Ala 
    210                 215                 220                 


Val Met Met Ser Gly Thr Leu Glu Thr Ile Thr Pro Gln Pro Pro Lys 
225                 230                 235                 240 


Trp His Ala Arg Leu Glu Glu Lys Val Ala Lys Val Thr Gly Lys Glu 
                245                 250                 255     


Val Ala Asp Leu Ala Ser Leu Ser Asp Lys Glu Leu Leu Asp Ala Gln 
            260                 265                 270         


Ile Lys Leu Asn Val Ala Val Cys Met Thr Cys Asp Asp Gly Asp Phe 
        275                 280                 285             


Phe Glu Pro Gly Trp Lys Gln His Leu Thr Pro Asp Trp Leu Asp Lys 
    290                 295                 300                 


Leu Ile Ile Ser Asp Cys Lys Asp Glu Gly Met Leu Tyr Phe Leu Pro 
305                 310                 315                 320 


Val Asn Ala Gln Asp Asp Glu Glu Leu Leu Ala Lys Val Ala Lys Ser 
                325                 330                 335     


Pro Val Gly Lys Glu Ile Ser Glu Leu Tyr Gly Ile Lys Glu Gly Gly 
            340                 345                 350         


Asp Ile Lys Ser Ala Cys Leu Asp Leu Lys Thr Asp Ala Thr Phe Asn 
        355                 360                 365             


Tyr Phe Asn His Leu Leu Phe Lys Lys Met Glu Glu Ala Arg Asn Asn 
    370                 375                 380                 


Gly Ser Thr Ser Arg Val Tyr Arg Leu Ala Val Asp Glu Pro Asn Pro 
385                 390                 395                 400 


His Asn Pro Asp Gln Arg Ala His His Ala Val Asp Val Leu Tyr Met 
                405                 410                 415     


Phe Asn Ser Thr Lys Phe Asn Glu His Gly Asp Lys Leu Ser Arg Leu 
            420                 425                 430         


Phe Gln Ser His Phe Leu Arg Leu Ala Tyr Gly Leu Glu Pro Trp Asp 
        435                 440                 445             


His Arg Asn Phe Gly Val Tyr Arg Asn Gly Gly Tyr Gln Gln Leu Pro 
    450                 455                 460                 


Leu Ser Glu Leu Asn Lys Val Arg Pro Val Glu Arg Tyr Glu Ala Leu 
465                 470                 475                 480 


Ser Lys Met Asp Phe Gly Gln Val Gly Arg Leu Ser Asn Ala Leu Ser 
                485                 490                 495     


Arg Leu 
        


<210>  86
<211>  406
<212>  PRT
<213>  Yarrowia lipolytica (GenBank Accession No. XP_503825)


<220>
<221>  MISC_FEATURE
<222>  (1)..(406)
<223>  Lip4a

<400>  86

Met Thr Phe Thr Ile Gly Ile Met Asn Leu Thr Leu Gly Gln Val Val 
1               5                   10                  15      


Ala Tyr Leu Tyr Ala Ser Leu Phe Gly Glu Pro Pro Ala Thr Leu Ser 
            20                  25                  30          


Lys Thr Arg Val Gln Ala Ser Gln Glu Leu Tyr Asn Phe Thr Ala Lys 
        35                  40                  45              


Phe Ser Arg Ile Ala Asn Ile Ala Tyr Cys Val Asn Ala Pro Val Thr 
    50                  55                  60                  


Pro Leu Arg Thr Asp Phe Thr Cys Gly Glu Ser Cys Arg Tyr Phe Pro 
65                  70                  75                  80  


Asn Met Thr Leu Asp Ser Val Phe Gly Gly Asp Phe Tyr Ser Thr Ser 
                85                  90                  95      


Ile Thr Gly Tyr Ile Ala Tyr Asp His Leu Asn Lys Glu Lys Tyr Val 
            100                 105                 110         


Val Ile Arg Gly Thr Phe Ser Ile Pro Asp Ala Val Thr Asp Ile Gln 
        115                 120                 125             


Phe Gln Gln Ser Pro Trp Leu Val Glu Leu Pro Lys His Leu Ile Pro 
    130                 135                 140                 


Thr Gln Asp Asp Met Lys Gln Arg Trp Ala Val Arg Pro Asp Leu Glu 
145                 150                 155                 160 


Val Glu Asn Lys Gly Leu Asp Asn Leu Lys Glu Arg Ala Leu Val Val 
                165                 170                 175     


Glu Asp Pro Arg Leu Ile Pro Ile Lys Thr Ser Glu Cys Lys Gly Cys 
            180                 185                 190         


Met Ile His Asp Gly Phe Ala Lys Ala Phe Asn Glu Thr Met Val Asn 
        195                 200                 205             


Ala Ala Pro Gln Phe Glu Lys Phe Leu Thr Asn His Thr Asp Tyr Lys 
    210                 215                 220                 


Met Tyr Val Thr Gly His Ser Leu Gly Ala Ala Gln Ala Leu Leu Phe 
225                 230                 235                 240 


Ala Thr His Phe Lys Leu Leu Gly His Asp Pro Thr Met Ile Asn Phe 
                245                 250                 255     


Gly Gln Pro Arg Val Gly Asn Ser Glu Phe Ala Asn Tyr Ile Asn Gln 
            260                 265                 270         


Leu Trp Phe Asn Asp Thr Gly Leu Glu Val Asn Asp Lys Arg Arg Asn 
        275                 280                 285             


Tyr Arg Leu Thr His Trp Asn Asp Ile Phe Val Gly Leu Pro Asp Trp 
    290                 295                 300                 


Ser Asn Tyr Thr His Ala Ile Gly Glu Val Tyr Ile Asp Gln Glu Ser 
305                 310                 315                 320 


Val Tyr Pro Ala Leu Asp Lys Val Ser Val Cys Glu Gly Gly Glu Asn 
                325                 330                 335     


Glu Ala Cys His Arg Gly Thr Phe Asn Leu Trp Ser Arg Ile Asp Leu 
            340                 345                 350         


Leu Gln Asn His Leu Ala Tyr Ile Tyr Tyr Ile Gly Tyr Cys Ala Leu 
        355                 360                 365             


Asn Ile Gly Arg Arg Asn Val Leu Ala Met Pro Lys Tyr His Gly Asn 
    370                 375                 380                 


Asn Ser Tyr Lys Tyr Ala Thr Glu Pro Gly Tyr Thr Gly Pro Gly Arg 
385                 390                 395                 400 


Thr Leu Val Pro Thr Asn 
                405     


<210>  87
<211>  390
<212>  DNA
<213>  Yarrowia lipolytica (GenBank Accession No. XM_503410)


<220>
<221>  CDS
<222>  (1)..(390)
<223>  SCP2 (YALI0E01298g)

<400>  87
atg tct ctc aag gtc gac ggc ttc act tct tct atc atc ttc gac gtc       48
Met Ser Leu Lys Val Asp Gly Phe Thr Ser Ser Ile Ile Phe Asp Val         
1               5                   10                  15              

atc cgt gac ggt ctt aac gac ccc tct cag gcc aag cag aag gct gag       96
Ile Arg Asp Gly Leu Asn Asp Pro Ser Gln Ala Lys Gln Lys Ala Glu         
            20                  25                  30                  

tcc atc aag aag gcc aac gcc atc att gtc ttc aac ctc aag aac aag      144
Ser Ile Lys Lys Ala Asn Ala Ile Ile Val Phe Asn Leu Lys Asn Lys         
        35                  40                  45                      

gct ggc aag acc gag tct tgg tac ctt gac ctc aag aac gac ggt gac      192
Ala Gly Lys Thr Glu Ser Trp Tyr Leu Asp Leu Lys Asn Asp Gly Asp         
    50                  55                  60                          

gtc ggc aag ggc aac aag tcc ccc aag ggt gat gct gac atc cag ctc      240
Val Gly Lys Gly Asn Lys Ser Pro Lys Gly Asp Ala Asp Ile Gln Leu         
65                  70                  75                  80          

act ctc tct gac gac cac ttc cag cag ctc gtt gag ggt aag gct aac      288
Thr Leu Ser Asp Asp His Phe Gln Gln Leu Val Glu Gly Lys Ala Asn         
                85                  90                  95              

gcc cag cga ctc ttc atg acc ggc aag ctc aag gtt aag ggc aac gtc      336
Ala Gln Arg Leu Phe Met Thr Gly Lys Leu Lys Val Lys Gly Asn Val         
            100                 105                 110                 

atg aag gct gcc gcc att gag ggt atc ctc aag aac gct cag aac aac      384
Met Lys Ala Ala Ala Ile Glu Gly Ile Leu Lys Asn Ala Gln Asn Asn         
        115                 120                 125                     

ctc taa                                                              390
Leu                                                                     
                                                                        


<210>  88
<211>  129
<212>  PRT
<213>  Yarrowia lipolytica (GenBank Accession No. XM_503410)

<400>  88

Met Ser Leu Lys Val Asp Gly Phe Thr Ser Ser Ile Ile Phe Asp Val 
1               5                   10                  15      


Ile Arg Asp Gly Leu Asn Asp Pro Ser Gln Ala Lys Gln Lys Ala Glu 
            20                  25                  30          


Ser Ile Lys Lys Ala Asn Ala Ile Ile Val Phe Asn Leu Lys Asn Lys 
        35                  40                  45              


Ala Gly Lys Thr Glu Ser Trp Tyr Leu Asp Leu Lys Asn Asp Gly Asp 
    50                  55                  60                  


Val Gly Lys Gly Asn Lys Ser Pro Lys Gly Asp Ala Asp Ile Gln Leu 
65                  70                  75                  80  


Thr Leu Ser Asp Asp His Phe Gln Gln Leu Val Glu Gly Lys Ala Asn 
                85                  90                  95      


Ala Gln Arg Leu Phe Met Thr Gly Lys Leu Lys Val Lys Gly Asn Val 
            100                 105                 110         


Met Lys Ala Ala Ala Ile Glu Gly Ile Leu Lys Asn Ala Gln Asn Asn 
        115                 120                 125             


Leu 
    


<210>  89
<211>  546
<212>  DNA
<213>  Yarrowia lipolytica (GenBank Accession No. XM_501987)


<220>
<221>  CDS
<222>  (1)..(546)
<223>  YALI0C18711g

<400>  89
atg cat cta cta caa cta agt tgt tca tca tca act ttc atc ttt caa       48
Met His Leu Leu Gln Leu Ser Cys Ser Ser Ser Thr Phe Ile Phe Gln         
1               5                   10                  15              

cac gat aca cca cat aac atg tcc acg cga gac acc ttt tac ggg tgg       96
His Asp Thr Pro His Asn Met Ser Thr Arg Asp Thr Phe Tyr Gly Trp         
            20                  25                  30                  

tat ttt gcc atc cac ttt gtc atc acc ctg atg gtc gat gga ggc ttt      144
Tyr Phe Ala Ile His Phe Val Ile Thr Leu Met Val Asp Gly Gly Phe         
        35                  40                  45                      

gcc att cct gcg gcg tac aaa cta ccc ggg caa cag ttc atg gaa gac      192
Ala Ile Pro Ala Ala Tyr Lys Leu Pro Gly Gln Gln Phe Met Glu Asp         
    50                  55                  60                          

ttc cac gtg gca tcc aac aag gat ttc att gtg ctc gaa aaa cca gcg      240
Phe His Val Ala Ser Asn Lys Asp Phe Ile Val Leu Glu Lys Pro Ala         
65                  70                  75                  80          

tgg ttc atg tcc ttc atc tgg atc gag atc ctc ttc cag ctg ccc ttc      288
Trp Phe Met Ser Phe Ile Trp Ile Glu Ile Leu Phe Gln Leu Pro Phe         
                85                  90                  95              

ttt gtt tat gga gcc tac aag ctg cta acc aag acc gcc act ccc acg      336
Phe Val Tyr Gly Ala Tyr Lys Leu Leu Thr Lys Thr Ala Thr Pro Thr         
            100                 105                 110                 

aca tac ctg tgg atg ctg gtg tac ggt gtc aat gcc agc cta acg act      384
Thr Tyr Leu Trp Met Leu Val Tyr Gly Val Asn Ala Ser Leu Thr Thr         
        115                 120                 125                     

ctg gca tgt ctc gcg gag gtg tgg gct cga gcc ggt ctt acc gat gcc      432
Leu Ala Cys Leu Ala Glu Val Trp Ala Arg Ala Gly Leu Thr Asp Ala         
    130                 135                 140                         

ctt cga tac aac ctg ctc gct gtc tac gcc ccc ttt ttc ttc att tct      480
Leu Arg Tyr Asn Leu Leu Ala Val Tyr Ala Pro Phe Phe Phe Ile Ser         
145                 150                 155                 160         

ggc tac att gtc att gac gta ttc cag cgg ttg cag gga gag ctg aga      528
Gly Tyr Ile Val Ile Asp Val Phe Gln Arg Leu Gln Gly Glu Leu Arg         
                165                 170                 175             

aag gtc aag act gat taa                                              546
Lys Val Lys Thr Asp                                                     
            180                                                         


<210>  90
<211>  181
<212>  PRT
<213>  Yarrowia lipolytica (GenBank Accession No. XM_501987)

<400>  90

Met His Leu Leu Gln Leu Ser Cys Ser Ser Ser Thr Phe Ile Phe Gln 
1               5                   10                  15      


His Asp Thr Pro His Asn Met Ser Thr Arg Asp Thr Phe Tyr Gly Trp 
            20                  25                  30          


Tyr Phe Ala Ile His Phe Val Ile Thr Leu Met Val Asp Gly Gly Phe 
        35                  40                  45              


Ala Ile Pro Ala Ala Tyr Lys Leu Pro Gly Gln Gln Phe Met Glu Asp 
    50                  55                  60                  


Phe His Val Ala Ser Asn Lys Asp Phe Ile Val Leu Glu Lys Pro Ala 
65                  70                  75                  80  


Trp Phe Met Ser Phe Ile Trp Ile Glu Ile Leu Phe Gln Leu Pro Phe 
                85                  90                  95      


Phe Val Tyr Gly Ala Tyr Lys Leu Leu Thr Lys Thr Ala Thr Pro Thr 
            100                 105                 110         


Thr Tyr Leu Trp Met Leu Val Tyr Gly Val Asn Ala Ser Leu Thr Thr 
        115                 120                 125             


Leu Ala Cys Leu Ala Glu Val Trp Ala Arg Ala Gly Leu Thr Asp Ala 
    130                 135                 140                 


Leu Arg Tyr Asn Leu Leu Ala Val Tyr Ala Pro Phe Phe Phe Ile Ser 
145                 150                 155                 160 


Gly Tyr Ile Val Ile Asp Val Phe Gln Arg Leu Gln Gly Glu Leu Arg 
                165                 170                 175     


Lys Val Lys Thr Asp 
            180     


<210>  91
<211>  1556
<212>  DNA
<213>  Yarrowia lipolytica (GenBank Accession No. XM_505819)


<220>
<221>  CDS
<222>  (501)..(1556)
<223>  YALI0F24167g

<400>  91
gctccaactt tcacgtggcg tcacacacac aggttcatgc acatcccgtt ttcaaagttg     60

cattgcatgc atttcgttcc cttagattga ccggaacaat tatcttggcg cactgagaat    120

gacctcacct cattatcggt gcagatcccg agagccgagg gtcggaggtc tctggtgagc    180

tcgagatgcg ttgcaatgac agctgggacg ggtccgcgac acacaggggg atatcctgct    240

gttctcgatg gctatctgtg ctgcccacta gtgcgccatt tctttccaca ccacacactg    300

agcctaggtt tttcgttttt ctgcttttgg gtggctccag ccggaccttc ccgctacagg    360

agccgaaaaa ccactgtttt ggattgagtt tcaagctcga actctacacc tggaaacttt    420

gctttgacag caaaatggat aagcgcaggt aatgaaaccc aatgcgggag tgagacaagc    480

acggataaat ttgggcgctg atg cct ata aaa gac ttc act aac cca gct ttt    533
                      Met Pro Ile Lys Asp Phe Thr Asn Pro Ala Phe       
                      1               5                   10            

tcg aac cca caa gag aca tcc tca cag cat aca cac aca aaa atg cct      581
Ser Asn Pro Gln Glu Thr Ser Ser Gln His Thr His Thr Lys Met Pro         
            15                  20                  25                  

tct gtc gcc gac aac act tcc aac ggt ccc att gag ggc aac gtt ctg      629
Ser Val Ala Asp Asn Thr Ser Asn Gly Pro Ile Glu Gly Asn Val Leu         
        30                  35                  40                      

ccc cag tcc aag ttc atc cag cac ctg agc gag tat cca gct gtt gct      677
Pro Gln Ser Lys Phe Ile Gln His Leu Ser Glu Tyr Pro Ala Val Ala         
    45                  50                  55                          

gcc gtg act ggc ttc gct gcc tct ttc ccc gtt gtc aag att ttt gca      725
Ala Val Thr Gly Phe Ala Ala Ser Phe Pro Val Val Lys Ile Phe Ala         
60                  65                  70                  75          

tcc aac gcc gtt cct ctc atc cag gcc atc cag aac cga ggt gcc ccc      773
Ser Asn Ala Val Pro Leu Ile Gln Ala Ile Gln Asn Arg Gly Ala Pro         
                80                  85                  90              

gtc gcc gag ccc gtc gtc aag cga gcc gcc ccc tac atc tct cag att      821
Val Ala Glu Pro Val Val Lys Arg Ala Ala Pro Tyr Ile Ser Gln Ile         
            95                  100                 105                 

gac aac gct gcc gac gag gcc ctc aac cga ctc gac aag gcc gtg ccc      869
Asp Asn Ala Ala Asp Glu Ala Leu Asn Arg Leu Asp Lys Ala Val Pro         
        110                 115                 120                     

tct ctc aag aac acc aag ccc gac gag gtc tac agc cga atc gtg acc      917
Ser Leu Lys Asn Thr Lys Pro Asp Glu Val Tyr Ser Arg Ile Val Thr         
    125                 130                 135                         

cag ccc ttg gag aac gtg cga ggt act gtc gac aag tac gcc gac gag      965
Gln Pro Leu Glu Asn Val Arg Gly Thr Val Asp Lys Tyr Ala Asp Glu         
140                 145                 150                 155         

acc aag aac act gtc tcc cga gtg gtt gtg cag ccc atc cga gac gtt     1013
Thr Lys Asn Thr Val Ser Arg Val Val Val Gln Pro Ile Arg Asp Val         
                160                 165                 170             

gcc tcc aga gtc cag tcc cag gtt gtc acc tac tac gat gcc cat gga     1061
Ala Ser Arg Val Gln Ser Gln Val Val Thr Tyr Tyr Asp Ala His Gly         
            175                 180                 185                 

aag ccc att gtc cac gcc cga ctc gac ccc atc ttc cac cct ctc aac     1109
Lys Pro Ile Val His Ala Arg Leu Asp Pro Ile Phe His Pro Leu Asn         
        190                 195                 200                     

gac cgt ctc gaa gcc ctg atc aac gcc tac ctg ccc aag ggc cag gag     1157
Asp Arg Leu Glu Ala Leu Ile Asn Ala Tyr Leu Pro Lys Gly Gln Glu         
    205                 210                 215                         

att gtg act gat gcc gag aac gag ctg gcc cga gca tgg cgc ctc acc     1205
Ile Val Thr Asp Ala Glu Asn Glu Leu Ala Arg Ala Trp Arg Leu Thr         
220                 225                 230                 235         

gtg gtt gct ttt gac cga gcc cga cct ctg att gag cag cag act tcc     1253
Val Val Ala Phe Asp Arg Ala Arg Pro Leu Ile Glu Gln Gln Thr Ser         
                240                 245                 250             

cag att cag gaa atc aac cag cac acc cga gaa cac atc cag aag gtt     1301
Gln Ile Gln Glu Ile Asn Gln His Thr Arg Glu His Ile Gln Lys Val         
            255                 260                 265                 

tac gac ggc aag cga tcg gaa atc gac gat aag aag act gtc tct ggt     1349
Tyr Asp Gly Lys Arg Ser Glu Ile Asp Asp Lys Lys Thr Val Ser Gly         
        270                 275                 280                     

cca gta tac gcc acc gtc gcc act gtc cga gat ctt tca cag gag ggc     1397
Pro Val Tyr Ala Thr Val Ala Thr Val Arg Asp Leu Ser Gln Glu Gly         
    285                 290                 295                         

ctc cag tac gcc cag tcc att ctg aat gcc aag aag ccc gag gag aag     1445
Leu Gln Tyr Ala Gln Ser Ile Leu Asn Ala Lys Lys Pro Glu Glu Lys         
300                 305                 310                 315         

gcc gac tct aac tct ggt gtt gct ccc gtg agc cag cct cac acc acc     1493
Ala Asp Ser Asn Ser Gly Val Ala Pro Val Ser Gln Pro His Thr Thr         
                320                 325                 330             

tcc gct gtt gac aac tcc ctc gct gct ccc acc gcc gtt cac gag gtt     1541
Ser Ala Val Asp Asn Ser Leu Ala Ala Pro Thr Ala Val His Glu Val         
            335                 340                 345                 

act gct tct gct taa                                                 1556
Thr Ala Ser Ala                                                         
        350                                                             


<210>  92
<211>  351
<212>  PRT
<213>  Yarrowia lipolytica (GenBank Accession No. XM_505819)

<400>  92

Met Pro Ile Lys Asp Phe Thr Asn Pro Ala Phe Ser Asn Pro Gln Glu 
1               5                   10                  15      


Thr Ser Ser Gln His Thr His Thr Lys Met Pro Ser Val Ala Asp Asn 
            20                  25                  30          


Thr Ser Asn Gly Pro Ile Glu Gly Asn Val Leu Pro Gln Ser Lys Phe 
        35                  40                  45              


Ile Gln His Leu Ser Glu Tyr Pro Ala Val Ala Ala Val Thr Gly Phe 
    50                  55                  60                  


Ala Ala Ser Phe Pro Val Val Lys Ile Phe Ala Ser Asn Ala Val Pro 
65                  70                  75                  80  


Leu Ile Gln Ala Ile Gln Asn Arg Gly Ala Pro Val Ala Glu Pro Val 
                85                  90                  95      


Val Lys Arg Ala Ala Pro Tyr Ile Ser Gln Ile Asp Asn Ala Ala Asp 
            100                 105                 110         


Glu Ala Leu Asn Arg Leu Asp Lys Ala Val Pro Ser Leu Lys Asn Thr 
        115                 120                 125             


Lys Pro Asp Glu Val Tyr Ser Arg Ile Val Thr Gln Pro Leu Glu Asn 
    130                 135                 140                 


Val Arg Gly Thr Val Asp Lys Tyr Ala Asp Glu Thr Lys Asn Thr Val 
145                 150                 155                 160 


Ser Arg Val Val Val Gln Pro Ile Arg Asp Val Ala Ser Arg Val Gln 
                165                 170                 175     


Ser Gln Val Val Thr Tyr Tyr Asp Ala His Gly Lys Pro Ile Val His 
            180                 185                 190         


Ala Arg Leu Asp Pro Ile Phe His Pro Leu Asn Asp Arg Leu Glu Ala 
        195                 200                 205             


Leu Ile Asn Ala Tyr Leu Pro Lys Gly Gln Glu Ile Val Thr Asp Ala 
    210                 215                 220                 


Glu Asn Glu Leu Ala Arg Ala Trp Arg Leu Thr Val Val Ala Phe Asp 
225                 230                 235                 240 


Arg Ala Arg Pro Leu Ile Glu Gln Gln Thr Ser Gln Ile Gln Glu Ile 
                245                 250                 255     


Asn Gln His Thr Arg Glu His Ile Gln Lys Val Tyr Asp Gly Lys Arg 
            260                 265                 270         


Ser Glu Ile Asp Asp Lys Lys Thr Val Ser Gly Pro Val Tyr Ala Thr 
        275                 280                 285             


Val Ala Thr Val Arg Asp Leu Ser Gln Glu Gly Leu Gln Tyr Ala Gln 
    290                 295                 300                 


Ser Ile Leu Asn Ala Lys Lys Pro Glu Glu Lys Ala Asp Ser Asn Ser 
305                 310                 315                 320 


Gly Val Ala Pro Val Ser Gln Pro His Thr Thr Ser Ala Val Asp Asn 
                325                 330                 335     


Ser Leu Ala Ala Pro Thr Ala Val His Glu Val Thr Ala Ser Ala 
            340                 345                 350     


<210>  93
<211>  2119
<212>  DNA
<213>  Yarrowia lipolytica


<220>
<221>  CDS
<222>  (291)..(1835)
<223>  DGAT2 opening reading frame, comprising 2 smaller internal 
       opening reading frames

<220>
<221>  misc_feature
<222>  (291)..(293)
<223>  initiation codon ('ATG')

<220>
<221>  misc_feature
<222>  (456)..(458)
<223>  initiation codon ('ATG')

<220>
<221>  misc_feature
<222>  (768)..(770)
<223>  initiation codon ('ATG')

<400>  93
aaacgcaccc actgctcgtc ctccttgctc ctcgaaaccg actcctctac acacgtcaaa     60

tccgaggttg aaatcttccc cacatttggc agccaaacca gcacatccca gcaacctcgc    120

acagcgccga aatcgacctg tcgacttggc cacaaaaaaa agcaccggct ctgcaacagt    180

tctcacgacc aattacgtac aagtacgaaa tcgttcgtgg accgtgactg ataagctccc    240

actttttctt ctaacaacag gcaacagaca agtcacacaa aacaaaagct atg act       296
                                                       Met Thr          
                                                       1                

atc gac tca caa tac tac aag tcg cga gac aaa aac gac acg gca ccc      344
Ile Asp Ser Gln Tyr Tyr Lys Ser Arg Asp Lys Asn Asp Thr Ala Pro         
        5                   10                  15                      

aaa atc gcg gga atc cga tat gcc ccg cta tcg aca cca tta ctc aac      392
Lys Ile Ala Gly Ile Arg Tyr Ala Pro Leu Ser Thr Pro Leu Leu Asn         
    20                  25                  30                          

cga tgt gag acc ttc tct ctg gtc tgg cac att ttc agc att ccc act      440
Arg Cys Glu Thr Phe Ser Leu Val Trp His Ile Phe Ser Ile Pro Thr         
35                  40                  45                  50          

ttc ctc aca att ttc atg cta tgc tgc gca att cca ctg ctc tgg cca      488
Phe Leu Thr Ile Phe Met Leu Cys Cys Ala Ile Pro Leu Leu Trp Pro         
                55                  60                  65              

ttt gtg att gcg tat gta gtg tac gct gtt aaa gac gac tcc ccg tcc      536
Phe Val Ile Ala Tyr Val Val Tyr Ala Val Lys Asp Asp Ser Pro Ser         
            70                  75                  80                  

aac gga gga gtg gtc aag cga tac tcg cct att tca aga aac ttc ttc      584
Asn Gly Gly Val Val Lys Arg Tyr Ser Pro Ile Ser Arg Asn Phe Phe         
        85                  90                  95                      

atc tgg aag ctc ttt ggc cgc tac ttc ccc ata act ctg cac aag acg      632
Ile Trp Lys Leu Phe Gly Arg Tyr Phe Pro Ile Thr Leu His Lys Thr         
    100                 105                 110                         

gtg gat ctg gag ccc acg cac aca tac tac cct ctg gac gtc cag gag      680
Val Asp Leu Glu Pro Thr His Thr Tyr Tyr Pro Leu Asp Val Gln Glu         
115                 120                 125                 130         

tat cac ctg att gct gag aga tac tgg ccg cag aac aag tac ctc cga      728
Tyr His Leu Ile Ala Glu Arg Tyr Trp Pro Gln Asn Lys Tyr Leu Arg         
                135                 140                 145             

gca atc atc tcc acc atc gag tac ttt ctg ccc gcc ttc atg aaa cgg      776
Ala Ile Ile Ser Thr Ile Glu Tyr Phe Leu Pro Ala Phe Met Lys Arg         
            150                 155                 160                 

tct ctt tct atc aac gag cag gag cag cct gcc gag cga gat cct ctc      824
Ser Leu Ser Ile Asn Glu Gln Glu Gln Pro Ala Glu Arg Asp Pro Leu         
        165                 170                 175                     

ctg tct ccc gtt tct ccc agc tct ccg ggt tct caa cct gac aag tgg      872
Leu Ser Pro Val Ser Pro Ser Ser Pro Gly Ser Gln Pro Asp Lys Trp         
    180                 185                 190                         

att aac cac gac agc aga tat agc cgt gga gaa tca tct ggc tcc aac      920
Ile Asn His Asp Ser Arg Tyr Ser Arg Gly Glu Ser Ser Gly Ser Asn         
195                 200                 205                 210         

ggc cac gcc tcg ggc tcc gaa ctt aac ggc aac ggc aac aat ggc acc      968
Gly His Ala Ser Gly Ser Glu Leu Asn Gly Asn Gly Asn Asn Gly Thr         
                215                 220                 225             

act aac cga cga cct ttg tcg tcc gcc tct gct ggc tcc act gca tct     1016
Thr Asn Arg Arg Pro Leu Ser Ser Ala Ser Ala Gly Ser Thr Ala Ser         
            230                 235                 240                 

gat tcc acg ctt ctt aac ggg tcc ctc aac tcc tac gcc aac cag atc     1064
Asp Ser Thr Leu Leu Asn Gly Ser Leu Asn Ser Tyr Ala Asn Gln Ile         
        245                 250                 255                     

att ggc gaa aac gac cca cag ctg tcg ccc aca aaa ctc aag ccc act     1112
Ile Gly Glu Asn Asp Pro Gln Leu Ser Pro Thr Lys Leu Lys Pro Thr         
    260                 265                 270                         

ggc aga aaa tac atc ttc ggc tac cac ccc cac ggc att atc ggc atg     1160
Gly Arg Lys Tyr Ile Phe Gly Tyr His Pro His Gly Ile Ile Gly Met         
275                 280                 285                 290         

gga gcc ttt ggt gga att gcc acc gag gga gct gga tgg tcc aag ctc     1208
Gly Ala Phe Gly Gly Ile Ala Thr Glu Gly Ala Gly Trp Ser Lys Leu         
                295                 300                 305             

ttt ccg ggc atc cct gtt tct ctt atg act ctc acc aac aac ttc cga     1256
Phe Pro Gly Ile Pro Val Ser Leu Met Thr Leu Thr Asn Asn Phe Arg         
            310                 315                 320                 

gtg cct ctc tac aga gag tac ctc atg agt ctg gga gtc gct tct gtc     1304
Val Pro Leu Tyr Arg Glu Tyr Leu Met Ser Leu Gly Val Ala Ser Val         
        325                 330                 335                     

tcc aag aag tcc tgc aag gcc ctc ctc aag cga aac cag tct atc tgc     1352
Ser Lys Lys Ser Cys Lys Ala Leu Leu Lys Arg Asn Gln Ser Ile Cys         
    340                 345                 350                         

att gtc gtt ggt gga gca cag gaa agt ctt ctg gcc aga ccc ggt gtc     1400
Ile Val Val Gly Gly Ala Gln Glu Ser Leu Leu Ala Arg Pro Gly Val         
355                 360                 365                 370         

atg gac ctg gtg cta ctc aag cga aag ggt ttt gtt cga ctt ggt atg     1448
Met Asp Leu Val Leu Leu Lys Arg Lys Gly Phe Val Arg Leu Gly Met         
                375                 380                 385             

gag gtc gga aat gtc gcc ctt gtt ccc atc atg gcc ttt ggt gag aac     1496
Glu Val Gly Asn Val Ala Leu Val Pro Ile Met Ala Phe Gly Glu Asn         
            390                 395                 400                 

gac ctc tat gac cag gtt agc aac gac aag tcg tcc aag ctg tac cga     1544
Asp Leu Tyr Asp Gln Val Ser Asn Asp Lys Ser Ser Lys Leu Tyr Arg         
        405                 410                 415                     

ttc cag cag ttt gtc aag aac ttc ctt gga ttc acc ctt cct ttg atg     1592
Phe Gln Gln Phe Val Lys Asn Phe Leu Gly Phe Thr Leu Pro Leu Met         
    420                 425                 430                         

cat gcc cga ggc gtc ttc aac tac gat gtc ggt ctt gtc ccc tac agg     1640
His Ala Arg Gly Val Phe Asn Tyr Asp Val Gly Leu Val Pro Tyr Arg         
435                 440                 445                 450         

cga ccc gtc aac att gtg gtt ggt tcc ccc att gac ttg cct tat ctc     1688
Arg Pro Val Asn Ile Val Val Gly Ser Pro Ile Asp Leu Pro Tyr Leu         
                455                 460                 465             

cca cac ccc acc gac gaa gaa gtg tcc gaa tac cac gac cga tac atc     1736
Pro His Pro Thr Asp Glu Glu Val Ser Glu Tyr His Asp Arg Tyr Ile         
            470                 475                 480                 

gcc gag ctg cag cga atc tac aac gag cac aag gat gaa tat ttc atc     1784
Ala Glu Leu Gln Arg Ile Tyr Asn Glu His Lys Asp Glu Tyr Phe Ile         
        485                 490                 495                     

gat tgg acc gag gag ggc aaa gga gcc cca gag ttc cga atg att gag     1832
Asp Trp Thr Glu Glu Gly Lys Gly Ala Pro Glu Phe Arg Met Ile Glu         
    500                 505                 510                         

taa ggaaaactgc ctgggttagg caaatagcta atgagtattt ttttgatggc          1885

aaccaaatgt agaaagaaaa aaaaaaaaaa agaaaaaaaa aagagaatat tatatctatg   1945

taattctatt aaaagctctg ttgagtgagc ggaataaata ctgttgaaga ggggattgtg   2005

tagagatctg tttactcaat ggcaaactca tctgggggag atccttccac tgtgggaagc   2065

tcctggatag cctttgcatc ggggttcaag aagaccattg tgaacagccc ttga         2119


<210>  94
<211>  514
<212>  PRT
<213>  Yarrowia lipolytica

<400>  94

Met Thr Ile Asp Ser Gln Tyr Tyr Lys Ser Arg Asp Lys Asn Asp Thr 
1               5                   10                  15      


Ala Pro Lys Ile Ala Gly Ile Arg Tyr Ala Pro Leu Ser Thr Pro Leu 
            20                  25                  30          


Leu Asn Arg Cys Glu Thr Phe Ser Leu Val Trp His Ile Phe Ser Ile 
        35                  40                  45              


Pro Thr Phe Leu Thr Ile Phe Met Leu Cys Cys Ala Ile Pro Leu Leu 
    50                  55                  60                  


Trp Pro Phe Val Ile Ala Tyr Val Val Tyr Ala Val Lys Asp Asp Ser 
65                  70                  75                  80  


Pro Ser Asn Gly Gly Val Val Lys Arg Tyr Ser Pro Ile Ser Arg Asn 
                85                  90                  95      


Phe Phe Ile Trp Lys Leu Phe Gly Arg Tyr Phe Pro Ile Thr Leu His 
            100                 105                 110         


Lys Thr Val Asp Leu Glu Pro Thr His Thr Tyr Tyr Pro Leu Asp Val 
        115                 120                 125             


Gln Glu Tyr His Leu Ile Ala Glu Arg Tyr Trp Pro Gln Asn Lys Tyr 
    130                 135                 140                 


Leu Arg Ala Ile Ile Ser Thr Ile Glu Tyr Phe Leu Pro Ala Phe Met 
145                 150                 155                 160 


Lys Arg Ser Leu Ser Ile Asn Glu Gln Glu Gln Pro Ala Glu Arg Asp 
                165                 170                 175     


Pro Leu Leu Ser Pro Val Ser Pro Ser Ser Pro Gly Ser Gln Pro Asp 
            180                 185                 190         


Lys Trp Ile Asn His Asp Ser Arg Tyr Ser Arg Gly Glu Ser Ser Gly 
        195                 200                 205             


Ser Asn Gly His Ala Ser Gly Ser Glu Leu Asn Gly Asn Gly Asn Asn 
    210                 215                 220                 


Gly Thr Thr Asn Arg Arg Pro Leu Ser Ser Ala Ser Ala Gly Ser Thr 
225                 230                 235                 240 


Ala Ser Asp Ser Thr Leu Leu Asn Gly Ser Leu Asn Ser Tyr Ala Asn 
                245                 250                 255     


Gln Ile Ile Gly Glu Asn Asp Pro Gln Leu Ser Pro Thr Lys Leu Lys 
            260                 265                 270         


Pro Thr Gly Arg Lys Tyr Ile Phe Gly Tyr His Pro His Gly Ile Ile 
        275                 280                 285             


Gly Met Gly Ala Phe Gly Gly Ile Ala Thr Glu Gly Ala Gly Trp Ser 
    290                 295                 300                 


Lys Leu Phe Pro Gly Ile Pro Val Ser Leu Met Thr Leu Thr Asn Asn 
305                 310                 315                 320 


Phe Arg Val Pro Leu Tyr Arg Glu Tyr Leu Met Ser Leu Gly Val Ala 
                325                 330                 335     


Ser Val Ser Lys Lys Ser Cys Lys Ala Leu Leu Lys Arg Asn Gln Ser 
            340                 345                 350         


Ile Cys Ile Val Val Gly Gly Ala Gln Glu Ser Leu Leu Ala Arg Pro 
        355                 360                 365             


Gly Val Met Asp Leu Val Leu Leu Lys Arg Lys Gly Phe Val Arg Leu 
    370                 375                 380                 


Gly Met Glu Val Gly Asn Val Ala Leu Val Pro Ile Met Ala Phe Gly 
385                 390                 395                 400 


Glu Asn Asp Leu Tyr Asp Gln Val Ser Asn Asp Lys Ser Ser Lys Leu 
                405                 410                 415     


Tyr Arg Phe Gln Gln Phe Val Lys Asn Phe Leu Gly Phe Thr Leu Pro 
            420                 425                 430         


Leu Met His Ala Arg Gly Val Phe Asn Tyr Asp Val Gly Leu Val Pro 
        435                 440                 445             


Tyr Arg Arg Pro Val Asn Ile Val Val Gly Ser Pro Ile Asp Leu Pro 
    450                 455                 460                 


Tyr Leu Pro His Pro Thr Asp Glu Glu Val Ser Glu Tyr His Asp Arg 
465                 470                 475                 480 


Tyr Ile Ala Glu Leu Gln Arg Ile Tyr Asn Glu His Lys Asp Glu Tyr 
                485                 490                 495     


Phe Ile Asp Trp Thr Glu Glu Gly Lys Gly Ala Pro Glu Phe Arg Met 
            500                 505                 510         


Ile Glu 
        


<210>  95
<211>  1024
<212>  PRT
<213>  Yarrowia lipolytica CLIB122 (GenBank Accession No. CAG82178)


<220>
<221>  MISC_FEATURE
<222>  (1)..(1024)
<223>  YlPex1p

<400>  95

Met Thr Ser Lys Ser Asp Tyr Ser Gly Lys Asp Lys Ile Glu Leu Asp 
1               5                   10                  15      


Pro Val Phe Ala Lys Ser Ile Asp Leu Leu Pro Asn Thr Gln Val Val 
            20                  25                  30          


Ile Asp Ile Gln Leu Asn Pro Lys Ile Ala His Thr Ile His Leu Glu 
        35                  40                  45              


Pro Val Thr Val Ala Asp Trp Glu Ile Val Glu Leu His Ala Ala Tyr 
    50                  55                  60                  


Leu Glu Ser Arg Met Ile Asn Gln Val Arg Ala Val Ser Pro Asn Gln 
65                  70                  75                  80  


Pro Val Thr Val Tyr Pro Ser Ser Thr Thr Ser Ala Thr Leu Lys Val 
                85                  90                  95      


Ile Arg Ile Glu Pro Asp Leu Gly Ala Ala Gly Phe Ala Lys Leu Ser 
            100                 105                 110         


Pro Asp Ser Glu Val Val Val Ala Pro Lys Gln Arg Lys Lys Glu Glu 
        115                 120                 125             


Lys Gln Val Lys Lys Arg Ser Gly Ser Ala Arg Ser Thr Gly Ser Gln 
    130                 135                 140                 


Lys Arg Lys Gly Gly Arg Gly Pro His Ala Leu Arg Arg Ala Ile Ser 
145                 150                 155                 160 


Glu Asp Phe Asp Gly His Leu Arg Leu Glu Val Ser Leu Asp Val Ser 
                165                 170                 175     


Gln Leu Pro Pro Glu Phe His Gln Leu Lys Asn Val Ser Ile Lys Val 
            180                 185                 190         


Ile Thr Pro Pro Asn Leu Ala Ser Pro Gln Gln Ala Ala Ser Ile Ala 
        195                 200                 205             


Val Glu Glu Lys Ser Glu Glu Ser Leu Ser Gln Asn Lys Pro Pro Ser 
    210                 215                 220                 


Ser Glu Pro Lys Val Glu Val Pro Pro Asp Ile Ile Asn Pro Ala Ser 
225                 230                 235                 240 


Glu Ile Val Ala Thr Leu Val Asn Asp Thr Thr Ser Pro Thr Gly His 
                245                 250                 255     


Ala Lys Leu Ser Tyr Ala Leu Ala Asp Ala Leu Gly Ile Pro Ser Ser 
            260                 265                 270         


Val Gly His Val Ile Arg Phe Glu Ser Ala Ser Lys Pro Leu Ser Gln 
        275                 280                 285             


Lys Pro Gly Ala Leu Val Ile His Arg Phe Ile Thr Lys Thr Val Gly 
    290                 295                 300                 


Ala Ala Glu Gln Lys Ser Leu Arg Leu Lys Gly Glu Lys Asn Ala Asp 
305                 310                 315                 320 


Asp Gly Val Ser Ala Asp Asp Gln Phe Ser Leu Leu Glu Glu Leu Lys 
                325                 330                 335     


Lys Leu Gln Met Leu Glu Gly Pro Ile Thr Asn Phe Gln Arg Leu Pro 
            340                 345                 350         


Pro Ile Pro Glu Leu Leu Pro Leu Gly Gly Val Ile Gly Leu Gln Asn 
        355                 360                 365             


Ser Glu Gly Trp Ile Gln Gly Gly Tyr Leu Gly Glu Glu Pro Ile Pro 
    370                 375                 380                 


Phe Val Ser Gly Ser Glu Ile Leu Arg Ser Glu Ser Ser Leu Ser Pro 
385                 390                 395                 400 


Ser Asn Ile Glu Ser Glu Asp Lys Arg Val Val Gly Leu Asp Asn Met 
                405                 410                 415     


Leu Asn Lys Ile Asn Glu Val Leu Ser Arg Asp Ser Ile Gly Cys Leu 
            420                 425                 430         


Val Tyr Gly Ser Arg Gly Ser Gly Lys Ser Ala Val Leu Asn His Ile 
        435                 440                 445             


Lys Lys Glu Cys Lys Val Ser His Thr His Thr Val Ser Ile Ala Cys 
    450                 455                 460                 


Gly Leu Ile Ala Gln Asp Arg Val Gln Ala Val Arg Glu Ile Leu Thr 
465                 470                 475                 480 


Lys Ala Phe Leu Glu Ala Ser Trp Phe Ser Pro Ser Val Leu Phe Leu 
                485                 490                 495     


Asp Asp Ile Asp Ala Leu Met Pro Ala Glu Val Glu His Ala Asp Ser 
            500                 505                 510         


Ser Arg Thr Arg Gln Leu Thr Gln Leu Phe Leu Glu Leu Ala Leu Pro 
        515                 520                 525             


Ile Met Lys Ser Arg His Val Ser Val Val Ala Ser Ala Gln Ala Lys 
    530                 535                 540                 


Glu Ser Leu His Met Asn Leu Val Thr Gly His Val Phe Glu Glu Leu 
545                 550                 555                 560 


Phe His Leu Lys Ser Pro Asp Lys Glu Ala Arg Leu Ala Ile Leu Ser 
                565                 570                 575     


Glu Ala Val Lys Leu Met Asp Gln Asn Val Ser Phe Ser Gln Asn Asp 
            580                 585                 590         


Val Leu Glu Ile Ala Ser Gln Val Asp Gly Tyr Leu Pro Gly Asp Leu 
        595                 600                 605             


Trp Thr Leu Ser Glu Arg Ala Gln His Glu Met Ala Leu Arg Gln Ile 
    610                 615                 620                 


Glu Ile Gly Leu Glu Asn Pro Ser Ile Gln Leu Ala Asp Phe Met Lys 
625                 630                 635                 640 


Ala Leu Glu Asp Phe Val Pro Ser Ser Leu Arg Gly Val Lys Leu Gln 
                645                 650                 655     


Lys Ser Asn Val Lys Trp Asn Asp Ile Gly Gly Leu Lys Glu Thr Lys 
            660                 665                 670         


Ala Val Leu Leu Glu Thr Leu Glu Trp Pro Thr Lys Tyr Ala Pro Ile 
        675                 680                 685             


Phe Ala Ser Cys Pro Leu Arg Leu Arg Ser Gly Leu Leu Leu Tyr Gly 
    690                 695                 700                 


Tyr Pro Gly Cys Gly Lys Thr Tyr Leu Ala Ser Ala Val Ala Ala Gln 
705                 710                 715                 720 


Cys Gly Leu Asn Phe Ile Ser Ile Lys Gly Pro Glu Ile Leu Asn Lys 
                725                 730                 735     


Tyr Ile Gly Ala Ser Glu Gln Ser Val Arg Glu Leu Phe Glu Arg Ala 
            740                 745                 750         


Gln Ala Ala Lys Pro Cys Ile Leu Phe Phe Asp Glu Phe Asp Ser Ile 
        755                 760                 765             


Ala Pro Lys Arg Gly His Asp Ser Thr Gly Val Thr Asp Arg Val Val 
    770                 775                 780                 


Asn Gln Met Leu Thr Gln Met Asp Gly Ala Glu Gly Leu Asp Gly Val 
785                 790                 795                 800 


Tyr Val Leu Ala Ala Thr Ser Arg Pro Asp Leu Ile Asp Pro Ala Leu 
                805                 810                 815     


Leu Arg Pro Gly Arg Leu Asp Lys Met Leu Ile Cys Asp Leu Pro Ser 
            820                 825                 830         


Tyr Glu Asp Arg Leu Asp Ile Leu Arg Ala Ile Val Asp Gly Lys Met 
        835                 840                 845             


His Leu Asp Gly Glu Val Glu Leu Glu Tyr Val Ala Ser Arg Thr Asp 
    850                 855                 860                 


Gly Phe Ser Gly Ala Asp Leu Gln Ala Val Met Phe Asn Ala Tyr Leu 
865                 870                 875                 880 


Glu Ala Ile His Glu Val Val Asp Val Ala Asp Asp Thr Ala Ala Asp 
                885                 890                 895     


Thr Pro Ala Leu Glu Asp Lys Arg Leu Glu Phe Phe Gln Thr Thr Leu 
            900                 905                 910         


Gly Asp Ala Lys Lys Asp Pro Ala Ala Val Gln Asn Glu Val Met Asn 
        915                 920                 925             


Ala Arg Ala Ala Val Ala Glu Lys Ala Arg Val Thr Ala Lys Leu Glu 
    930                 935                 940                 


Ala Leu Phe Lys Gly Met Ser Val Gly Val Asp Asn Asp Asp Asp Lys 
945                 950                 955                 960 


Pro Arg Lys Lys Ala Val Val Val Ile Lys Pro Gln His Met Asn Lys 
                965                 970                 975     


Ser Leu Asp Glu Thr Ser Pro Ser Ile Ser Lys Lys Glu Leu Leu Lys 
            980                 985                 990         


Leu Lys Gly Ile Tyr Ser Gln Phe  Val Ser Gly Arg Ser  Gly Asp Met 
        995                 1000                 1005             


Pro Pro  Gly Thr Ala Ser Thr  Asp Val Gly Gly Arg  Ala Thr Leu 
    1010                 1015                 1020             


Ala 
    


<210>  96
<211>  381
<212>  PRT
<213>  Yarrowia lipolytica CLIB122 (GenBank Accession No. CAG77647)


<220>
<221>  MISC_FEATURE
<222>  (1)..(381)
<223>  YlPex2p

<400>  96

Met Ser Ser Val Leu Arg Leu Phe Lys Ile Gly Ala Pro Val Pro Asn 
1               5                   10                  15      


Val Arg Val His Gln Leu Asp Ala Ser Leu Leu Asp Ala Glu Leu Val 
            20                  25                  30          


Asp Leu Leu Lys Asn Gln Leu Phe Lys Gly Phe Thr Asn Phe His Pro 
        35                  40                  45              


Glu Phe Arg Asp Lys Tyr Glu Ser Glu Leu Val Leu Ala Leu Lys Leu 
    50                  55                  60                  


Ile Leu Phe Lys Leu Thr Val Trp Asp His Ala Ile Thr Tyr Gly Gly 
65                  70                  75                  80  


Lys Leu Gln Asn Leu Lys Phe Ile Asp Ser Arg His Ser Ser Lys Leu 
                85                  90                  95      


Gln Ile Gln Pro Ser Val Ile Gln Lys Leu Gly Tyr Gly Ile Leu Val 
            100                 105                 110         


Val Gly Gly Gly Tyr Leu Trp Ser Lys Ile Glu Gly Tyr Leu Leu Ala 
        115                 120                 125             


Arg Ser Glu Asp Asp Val Ala Thr Asp Gly Thr Ser Val Arg Gly Ala 
    130                 135                 140                 


Ser Ala Ala Arg Gly Ala Leu Lys Val Ala Asn Phe Ala Ser Leu Leu 
145                 150                 155                 160 


Tyr Ser Ala Ala Thr Leu Gly Asn Phe Val Ala Phe Leu Tyr Thr Gly 
                165                 170                 175     


Arg Tyr Ala Thr Val Ile Met Arg Leu Leu Arg Ile Arg Leu Val Pro 
            180                 185                 190         


Ser Gln Arg Thr Ser Ser Arg Gln Val Ser Tyr Glu Phe Gln Asn Arg 
        195                 200                 205             


Gln Leu Val Trp Asn Ala Phe Thr Glu Phe Leu Ile Phe Ile Leu Pro 
    210                 215                 220                 


Leu Leu Gln Leu Pro Lys Leu Lys Arg Arg Ile Glu Arg Lys Leu Gln 
225                 230                 235                 240 


Ser Leu Asn Val Thr Arg Val Gly Asn Val Glu Glu Ala Ser Glu Gly 
                245                 250                 255     


Glu Leu Ala His Leu Pro Gln Lys Thr Cys Ala Ile Cys Phe Arg Asp 
            260                 265                 270         


Glu Glu Glu Gln Glu Gly Gly Gly Gly Ala Ser His Tyr Ser Thr Asp 
        275                 280                 285             


Val Thr Asn Pro Tyr Gln Ala Asp Cys Gly His Val Tyr Cys Tyr Val 
    290                 295                 300                 


Cys Leu Val Thr Lys Leu Ala Gln Gly Asp Gly Asp Gly Trp Asn Cys 
305                 310                 315                 320 


Tyr Arg Cys Ala Lys Gln Val Gln Lys Met Lys Pro Trp Val Asp Val 
                325                 330                 335     


Asp Glu Ala Ala Val Val Gly Ala Ala Glu Met His Glu Lys Val Asp 
            340                 345                 350         


Val Ile Glu His Ala Glu Asp Asn Glu Gln Glu Glu Glu Glu Phe Asp 
        355                 360                 365             


Asp Asp Asp Glu Asp Ser Asn Phe Gln Leu Met Lys Asp 
    370                 375                 380     


<210>  97
<211>  431
<212>  PRT
<213>  Yarrowia lipolytica CLIB122 (GenBank Accession No. CAG78565)


<220>
<221>  MISC_FEATURE
<222>  (1)..(431)
<223>  YlPex3p

<400>  97

Met Asp Phe Phe Arg Arg His Gln Lys Lys Val Leu Ala Leu Val Gly 
1               5                   10                  15      


Val Ala Leu Ser Ser Tyr Leu Phe Ile Asp Tyr Val Lys Lys Lys Phe 
            20                  25                  30          


Phe Glu Ile Gln Gly Arg Leu Ser Ser Glu Arg Thr Ala Lys Gln Asn 
        35                  40                  45              


Leu Arg Arg Arg Phe Glu Gln Asn Gln Gln Asp Ala Asp Phe Thr Ile 
    50                  55                  60                  


Met Ala Leu Leu Ser Ser Leu Thr Thr Pro Val Met Glu Arg Tyr Pro 
65                  70                  75                  80  


Val Asp Gln Ile Lys Ala Glu Leu Gln Ser Lys Arg Arg Pro Thr Asp 
                85                  90                  95      


Arg Val Leu Ala Leu Glu Ser Ser Thr Ser Ser Ser Ala Thr Ala Gln 
            100                 105                 110         


Thr Val Pro Thr Met Thr Ser Gly Ala Thr Glu Glu Gly Glu Lys Ser 
        115                 120                 125             


Lys Thr Gln Leu Trp Gln Asp Leu Lys Arg Thr Thr Ile Ser Arg Ala 
    130                 135                 140                 


Phe Ser Leu Val Tyr Ala Asp Ala Leu Leu Ile Phe Phe Thr Arg Leu 
145                 150                 155                 160 


Gln Leu Asn Ile Leu Gly Arg Arg Asn Tyr Val Asn Ser Val Val Ala 
                165                 170                 175     


Leu Ala Gln Gln Gly Arg Glu Gly Asn Ala Glu Gly Arg Val Ala Pro 
            180                 185                 190         


Ser Phe Gly Asp Leu Ala Asp Met Gly Tyr Phe Gly Asp Leu Ser Gly 
        195                 200                 205             


Ser Ser Ser Phe Gly Glu Thr Ile Val Asp Pro Asp Leu Asp Glu Gln 
    210                 215                 220                 


Tyr Leu Thr Phe Ser Trp Trp Leu Leu Asn Glu Gly Trp Val Ser Leu 
225                 230                 235                 240 


Ser Glu Arg Val Glu Glu Ala Val Arg Arg Val Trp Asp Pro Val Ser 
                245                 250                 255     


Pro Lys Ala Glu Leu Gly Phe Asp Glu Leu Ser Glu Leu Ile Gly Arg 
            260                 265                 270         


Thr Gln Met Leu Ile Asp Arg Pro Leu Asn Pro Ser Ser Pro Leu Asn 
        275                 280                 285             


Phe Leu Ser Gln Leu Leu Pro Pro Arg Glu Gln Glu Glu Tyr Val Leu 
    290                 295                 300                 


Ala Gln Asn Pro Ser Asp Thr Ala Ala Pro Ile Val Gly Pro Thr Leu 
305                 310                 315                 320 


Arg Arg Leu Leu Asp Glu Thr Ala Asp Phe Ile Glu Ser Pro Asn Ala 
                325                 330                 335     


Ala Glu Val Ile Glu Arg Leu Val His Ser Gly Leu Ser Val Phe Met 
            340                 345                 350         


Asp Lys Leu Ala Val Thr Phe Gly Ala Thr Pro Ala Asp Ser Gly Ser 
        355                 360                 365             


Pro Tyr Pro Val Val Leu Pro Thr Ala Lys Val Lys Leu Pro Ser Ile 
    370                 375                 380                 


Leu Ala Asn Met Ala Arg Gln Ala Gly Gly Met Ala Gln Gly Ser Pro 
385                 390                 395                 400 


Gly Val Glu Asn Glu Tyr Ile Asp Val Met Asn Gln Val Gln Glu Leu 
                405                 410                 415     


Thr Ser Phe Ser Ala Val Val Tyr Ser Ser Phe Asp Trp Ala Leu 
            420                 425                 430     


<210>  98
<211>  395
<212>  PRT
<213>  Yarrowia lipolytica CLIB122 (GenBank Accession No. CAG83356)


<220>
<221>  MISC_FEATURE
<222>  (1)..(395)
<223>  YlPex3Bp

<400>  98

Met Leu Gln Ser Leu Asn Arg Asn Lys Lys Arg Leu Ala Val Ser Thr 
1               5                   10                  15      


Gly Leu Ile Ala Val Ala Tyr Val Val Ile Ser Tyr Thr Thr Lys Arg 
            20                  25                  30          


Leu Ile Glu Lys Gln Glu Gln Lys Leu Glu Glu Glu Arg Ala Lys Glu 
        35                  40                  45              


Arg Leu Lys Gln Leu Phe Ala Gln Thr Gln Asn Glu Ala Ala Phe His 
    50                  55                  60                  


Thr Ala Ser Val Leu Pro Gln Leu Cys Glu Gln Ile Met Glu Phe Val 
65                  70                  75                  80  


Ala Val Glu Lys Ile Ala Glu Gln Leu Gln Asn Met Arg Ala Glu Lys 
                85                  90                  95      


Arg Lys Lys Gln Asn Met Asp Asp Asp Lys His Ser Val Leu Ser Leu 
            100                 105                 110         


Gly Thr Glu Thr Thr Ala Ser Met Ala Asp Gly Gln Lys Met Ser Lys 
        115                 120                 125             


Ile Gln Leu Trp Asp Glu Leu Lys Ile Glu Ser Leu Thr Arg Ile Val 
    130                 135                 140                 


Thr Leu Ile Tyr Cys Val Ser Leu Leu Asn Tyr Leu Ile Arg Leu Gln 
145                 150                 155                 160 


Thr Asn Ile Val Gly Arg Lys Arg Tyr Gln Asn Glu Ala Gly Pro Ala 
                165                 170                 175     


Gly Ala Thr Tyr Asp Met Ser Leu Glu Gln Cys Tyr Thr Trp Leu Leu 
            180                 185                 190         


Thr Arg Gly Trp Lys Ser Val Val Asp Asn Val Arg Arg Ser Val Gln 
        195                 200                 205             


Gln Val Phe Thr Gly Val Asn Pro Arg Gln Asn Leu Ser Leu Asp Glu 
    210                 215                 220                 


Phe Ala Thr Leu Leu Lys Arg Val Gln Thr Leu Val Asn Ser Pro Pro 
225                 230                 235                 240 


Tyr Ser Thr Thr Pro Asn Thr Phe Leu Thr Ser Leu Leu Pro Pro Arg 
                245                 250                 255     


Glu Leu Glu Gln Leu Arg Leu Glu Lys Glu Lys Gln Ser Leu Ser Pro 
            260                 265                 270         


Asn Tyr Thr Tyr Gly Ser Pro Leu Lys Asp Leu Val Phe Glu Ser Ala 
        275                 280                 285             


Gln His Ile Gln Ser Pro Gln Gly Met Ser Ser Phe Arg Ala Ile Ile 
    290                 295                 300                 


Asp Gln Ser Phe Lys Val Phe Leu Glu Lys Val Asn Glu Ser Gln Tyr 
305                 310                 315                 320 


Val Asn Pro Pro Ser Thr Gly Gly Lys Arg Ile Ala Val Gly Ala Leu 
                325                 330                 335     


Gln Pro Pro Ile Ile Ser Gly Gly Pro Lys Lys Val Lys Leu Ala Ser 
            340                 345                 350         


Leu Leu Ser Val Ala Thr Arg Gln Ser Ser Val Ile Ser His Ala Gln 
        355                 360                 365             


Pro Asn Pro Tyr Val Asp Ala Ile Asn Ser Val Ala Glu Tyr Asn Gly 
    370                 375                 380                 


Leu Cys Ala Val Ile Tyr Ser Ser Phe Glu Gln 
385                 390                 395 


<210>  99
<211>  153
<212>  PRT
<213>  Yarrowia lipolytica CLIB122 (GenBank Accession No. CAG79130)


<220>
<221>  MISC_FEATURE
<222>  (1)..(153)
<223>  YlPex4p

<400>  99

Met Ala Ser Gln Lys Arg Leu Ile Lys Glu Leu Ala Ala Tyr Lys Lys 
1               5                   10                  15      


Asp Pro Asn Pro Cys Leu Ala Ser Leu Thr Ala Asp Gly Asp Ser Leu 
            20                  25                  30          


Tyr Lys Trp Thr Ala Val Met Arg Gly Thr Glu Gly Thr Ala Tyr Glu 
        35                  40                  45              


Asn Gly Leu Trp Gln Val Glu Ile Asn Ile Pro Glu Asn Tyr Pro Leu 
    50                  55                  60                  


Gln Pro Pro Thr Met Phe Phe Arg Thr Lys Ile Cys His Pro Asn Ile 
65                  70                  75                  80  


His Phe Glu Thr Gly Glu Val Cys Ile Asp Val Leu Lys Thr Gln Trp 
                85                  90                  95      


Ser Pro Ala Trp Thr Ile Ser Ser Ala Cys Thr Ala Val Ser Ala Met 
            100                 105                 110         


Leu Ser Leu Pro Glu Pro Asp Ser Pro Leu Asn Ile Asp Ala Ala Asn 
        115                 120                 125             


Leu Val Arg Cys Gly Asp Glu Ser Ala Met Glu Gly Leu Val Arg Tyr 
    130                 135                 140                 


Tyr Val Asn Lys Tyr Ala Ser Gly Asn 
145                 150             


<210>  100
<211>  598
<212>  PRT
<213>  Yarrowia lipolytica CLIB122 (GenBank Accession No. CAG78803)


<220>
<221>  MISC_FEATURE
<222>  (1)..(598)
<223>  YlPex5p

<400>  100

Met Ser Phe Met Arg Gly Gly Ser Glu Cys Ser Thr Gly Arg Asn Pro 
1               5                   10                  15      


Leu Ser Gln Phe Thr Lys His Thr Ala Glu Asp Arg Ser Leu Gln His 
            20                  25                  30          


Asp Arg Val Ala Gly Pro Ser Gly Gly Arg Val Gly Gly Met Arg Ser 
        35                  40                  45              


Asn Thr Gly Glu Met Ser Gln Gln Asp Arg Glu Met Met Ala Arg Phe 
    50                  55                  60                  


Gly Ala Ala Gly Pro Glu Gln Ser Ser Phe Asn Tyr Glu Gln Met Arg 
65                  70                  75                  80  


His Glu Leu His Asn Met Gly Ala Gln Gly Gly Gln Ile Pro Gln Val 
                85                  90                  95      


Pro Ser Gln Gln Gly Ala Ala Asn Gly Gly Gln Trp Ala Arg Asp Phe 
            100                 105                 110         


Gly Gly Gln Gln Thr Ala Pro Gly Ala Ala Pro Gln Asp Ala Lys Asn 
        115                 120                 125             


Trp Asn Ala Glu Phe Gln Arg Gly Gly Ser Pro Ala Glu Ala Met Gln 
    130                 135                 140                 


Gln Gln Gly Pro Gly Pro Met Gln Gly Gly Met Gly Met Gly Gly Met 
145                 150                 155                 160 


Pro Met Tyr Gly Met Ala Arg Pro Met Tyr Ser Gly Met Ser Ala Asn 
                165                 170                 175     


Met Ala Pro Gln Phe Gln Pro Gln Gln Ala Asn Ala Arg Val Val Glu 
            180                 185                 190         


Leu Asp Glu Gln Asn Trp Glu Glu Gln Phe Lys Gln Met Asp Ser Ala 
        195                 200                 205             


Val Gly Lys Gly Lys Glu Val Glu Glu Gln Thr Ala Glu Thr Ala Thr 
    210                 215                 220                 


Ala Thr Glu Thr Val Thr Glu Thr Glu Thr Thr Thr Glu Asp Lys Pro 
225                 230                 235                 240 


Met Asp Ile Lys Asn Met Asp Phe Glu Asn Ile Trp Lys Asn Leu Gln 
                245                 250                 255     


Val Asn Val Leu Asp Asn Met Asp Glu Trp Leu Glu Glu Thr Asn Ser 
            260                 265                 270         


Pro Ala Trp Glu Arg Asp Phe His Glu Tyr Thr His Asn Arg Pro Glu 
        275                 280                 285             


Phe Ala Asp Tyr Gln Phe Glu Glu Asn Asn Gln Phe Met Glu His Pro 
    290                 295                 300                 


Asp Pro Phe Lys Ile Gly Val Glu Leu Met Glu Thr Gly Gly Arg Leu 
305                 310                 315                 320 


Ser Glu Ala Ala Leu Ala Phe Glu Ala Ala Val Gln Lys Asn Thr Glu 
                325                 330                 335     


His Ala Glu Ala Trp Gly Arg Leu Gly Ala Cys Gln Ala Gln Asn Glu 
            340                 345                 350         


Lys Glu Asp Pro Ala Ile Arg Ala Leu Glu Arg Cys Ile Lys Leu Glu 
        355                 360                 365             


Pro Gly Asn Leu Ser Ala Leu Met Asn Leu Ser Val Ser Tyr Thr Asn 
    370                 375                 380                 


Glu Gly Tyr Glu Asn Ala Ala Tyr Ala Thr Leu Glu Arg Trp Leu Ala 
385                 390                 395                 400 


Thr Lys Tyr Pro Glu Val Val Asp Gln Ala Arg Asn Gln Glu Pro Arg 
                405                 410                 415     


Leu Gly Asn Glu Asp Lys Phe Gln Leu His Ser Arg Val Thr Glu Leu 
            420                 425                 430         


Phe Ile Arg Ala Ala Gln Leu Ser Pro Asp Gly Ala Asn Ile Asp Ala 
        435                 440                 445             


Asp Val Gln Val Gly Leu Gly Val Leu Phe Tyr Gly Asn Glu Glu Tyr 
    450                 455                 460                 


Asp Lys Ala Ile Asp Cys Phe Asn Ala Ala Ile Ala Val Arg Pro Asp 
465                 470                 475                 480 


Asp Ala Leu Leu Trp Asn Arg Leu Gly Ala Thr Leu Ala Asn Ser His 
                485                 490                 495     


Arg Ser Glu Glu Ala Ile Asp Ala Tyr Tyr Lys Ala Leu Glu Leu Arg 
            500                 505                 510         


Pro Ser Phe Val Arg Ala Arg Tyr Asn Leu Gly Val Ser Cys Ile Asn 
        515                 520                 525             


Ile Gly Cys Tyr Lys Glu Ala Ala Gln Tyr Leu Leu Gly Ala Leu Ser 
    530                 535                 540                 


Met His Lys Val Glu Gly Val Gln Asp Asp Val Leu Ala Asn Gln Ser 
545                 550                 555                 560 


Thr Asn Leu Tyr Asp Thr Leu Lys Arg Val Phe Leu Gly Met Asp Arg 
                565                 570                 575     


Arg Asp Leu Val Ala Lys Val Gly Asn Gly Met Asp Val Asn Gln Phe 
            580                 585                 590         


Arg Asn Glu Phe Glu Phe 
        595             


<210>  101
<211>  1024
<212>  PRT
<213>  Yarrowia lipolytica CLIB122 (GenBank Accession No. CAG82306)


<220>
<221>  MISC_FEATURE
<222>  (1)..(1024)
<223>  YlPex6p

<400>  101

Met Pro Ser Ile Ser His Lys Pro Ile Thr Ala Lys Leu Val Ala Ala 
1               5                   10                  15      


Pro Asp Ala Thr Lys Leu Glu Leu Ser Ser Tyr Leu Tyr Gln Gln Leu 
            20                  25                  30          


Phe Ser Asp Lys Pro Ala Glu Pro Tyr Val Ala Phe Glu Ala Pro Gly 
        35                  40                  45              


Ile Lys Trp Ala Leu Tyr Pro Ala Ser Glu Asp Arg Ser Leu Pro Gln 
    50                  55                  60                  


Tyr Thr Cys Lys Ala Asp Ile Arg His Val Ala Gly Ser Leu Lys Lys 
65                  70                  75                  80  


Phe Met Pro Val Val Leu Lys Arg Val Asn Pro Val Thr Ile Glu His 
                85                  90                  95      


Ala Ile Val Thr Val Pro Ala Ser Gln Tyr Glu Thr Leu Asn Thr Pro 
            100                 105                 110         


Glu Gln Val Leu Lys Ala Leu Glu Pro Gln Leu Asp Lys Asp Arg Pro 
        115                 120                 125             


Val Ile Arg Gln Gly Asp Val Leu Leu Asn Gly Cys Arg Val Arg Leu 
    130                 135                 140                 


Cys Glu Pro Val Asn Gln Gly Lys Val Val Lys Gly Thr Thr Lys Leu 
145                 150                 155                 160 


Thr Val Ala Lys Glu Gln Glu Thr Ile Gln Pro Ala Asp Glu Ala Ala 
                165                 170                 175     


Asp Val Ala Phe Asp Ile Ala Glu Phe Leu Asp Phe Asp Thr Ser Val 
            180                 185                 190         


Ala Lys Thr Arg Glu Ser Thr Asn Leu Gln Val Ala Pro Leu Glu Gly 
        195                 200                 205             


Ala Ile Pro Thr Pro Leu Ser Asp Arg Phe Asp Asp Cys Glu Ser Arg 
    210                 215                 220                 


Gly Phe Val Lys Ser Glu Thr Met Ser Lys Leu Gly Val Phe Ser Gly 
225                 230                 235                 240 


Asp Ile Val Ser Ile Lys Thr Lys Asn Gly Ala Glu Arg Val Leu Arg 
                245                 250                 255     


Leu Phe Ala Tyr Pro Glu Pro Asn Thr Val Lys Tyr Asp Val Val Tyr 
            260                 265                 270         


Val Ser Pro Ile Leu Tyr His Asn Ile Gly Asp Lys Glu Ile Glu Val 
        275                 280                 285             


Thr Pro Asn Gly Glu Thr His Lys Ser Val Gly Glu Ala Leu Asp Ser 
    290                 295                 300                 


Val Leu Glu Ala Ala Glu Glu Val Lys Leu Ala Arg Val Leu Gly Pro 
305                 310                 315                 320 


Thr Thr Thr Asp Arg Thr Phe Gln Thr Ala Tyr His Ala Gly Leu Gln 
                325                 330                 335     


Ala Tyr Phe Lys Pro Val Lys Arg Ala Val Arg Val Gly Asp Leu Ile 
            340                 345                 350         


Pro Ile Pro Phe Asp Ser Ile Leu Ala Arg Thr Ile Gly Glu Asp Pro 
        355                 360                 365             


Glu Met Ser His Ile Pro Leu Glu Ala Leu Ala Val Lys Pro Asp Ser 
    370                 375                 380                 


Val Ala Trp Phe Gln Val Thr Ser Leu Asn Gly Ser Glu Asp Pro Ala 
385                 390                 395                 400 


Ser Lys Gln Tyr Leu Val Asp Ser Ser Gln Thr Lys Leu Ile Glu Gly 
                405                 410                 415     


Gly Thr Thr Ser Ser Ala Val Ile Pro Thr Ser Val Pro Trp Arg Glu 
            420                 425                 430         


Tyr Leu Gly Leu Asp Thr Leu Pro Lys Phe Gly Ser Glu Phe Ala Tyr 
        435                 440                 445             


Ala Asp Lys Ile Arg Asn Leu Val Gln Ile Ser Thr Ser Ala Leu Ser 
    450                 455                 460                 


His Ala Lys Leu Asn Thr Ser Val Leu Leu His Ser Ala Lys Arg Gly 
465                 470                 475                 480 


Val Gly Lys Ser Thr Val Leu Arg Ser Val Ala Ala Gln Cys Gly Ile 
                485                 490                 495     


Ser Val Phe Glu Ile Ser Cys Phe Gly Leu Ile Gly Asp Asn Glu Ala 
            500                 505                 510         


Gln Thr Leu Gly Thr Leu Arg Ala Lys Leu Asp Arg Ala Tyr Gly Cys 
        515                 520                 525             


Ser Pro Cys Val Val Val Leu Gln His Leu Glu Ser Ile Ala Lys Lys 
    530                 535                 540                 


Ser Asp Gln Asp Gly Lys Asp Glu Gly Ile Val Ser Lys Leu Val Asp 
545                 550                 555                 560 


Val Leu Ala Asp Tyr Ser Gly His Gly Val Leu Leu Ala Ala Thr Ser 
                565                 570                 575     


Asn Asp Pro Asp Lys Ile Ser Glu Ala Ile Arg Ser Arg Phe Gln Phe 
            580                 585                 590         


Glu Ile Glu Ile Gly Val Pro Ser Glu Pro Gln Arg Arg Gln Ile Phe 
        595                 600                 605             


Ser His Leu Thr Lys Ser Gly Pro Gly Gly Asp Ser Ile Arg Asn Ala 
    610                 615                 620                 


Pro Ile Ser Leu Arg Ser Asp Val Ser Val Glu Asn Leu Ala Leu Gln 
625                 630                 635                 640 


Ser Ala Gly Leu Thr Pro Pro Asp Leu Thr Ala Ile Val Gln Thr Thr 
                645                 650                 655     


Arg Leu Arg Ala Ile Asp Arg Leu Asn Lys Leu Thr Lys Asp Ser Asp 
            660                 665                 670         


Thr Thr Leu Asp Asp Leu Leu Thr Leu Ser His Gly Thr Leu Gln Leu 
        675                 680                 685             


Thr Pro Ser Asp Phe Asp Asp Ala Ile Ala Asp Ala Arg Gln Lys Tyr 
    690                 695                 700                 


Ser Asp Ser Ile Gly Ala Pro Arg Ile Pro Asn Val Gly Trp Asp Asp 
705                 710                 715                 720 


Val Gly Gly Met Glu Gly Val Lys Lys Asp Ile Leu Asp Thr Ile Glu 
                725                 730                 735     


Thr Pro Leu Lys Tyr Pro His Trp Phe Ser Asp Gly Val Lys Lys Arg 
            740                 745                 750         


Ser Gly Ile Leu Phe Tyr Gly Pro Pro Gly Thr Gly Lys Thr Leu Leu 
        755                 760                 765             


Ala Lys Ala Ile Ala Thr Thr Phe Ser Leu Asn Phe Phe Ser Val Lys 
    770                 775                 780                 


Gly Pro Glu Leu Leu Asn Met Tyr Ile Gly Glu Ser Glu Ala Asn Val 
785                 790                 795                 800 


Arg Arg Val Phe Gln Lys Ala Arg Asp Ala Lys Pro Cys Val Val Phe 
                805                 810                 815     


Phe Asp Glu Leu Asp Ser Val Ala Pro Gln Arg Gly Asn Gln Gly Asp 
            820                 825                 830         


Ser Gly Gly Val Met Asp Arg Ile Val Ser Gln Leu Leu Ala Glu Leu 
        835                 840                 845             


Asp Gly Met Ser Thr Ala Gly Gly Glu Gly Val Phe Val Val Gly Ala 
    850                 855                 860                 


Thr Asn Arg Pro Asp Leu Leu Asp Glu Ala Leu Leu Arg Pro Gly Arg 
865                 870                 875                 880 


Phe Asp Lys Met Leu Tyr Leu Gly Ile Ser Asp Thr His Glu Lys Gln 
                885                 890                 895     


Gln Thr Ile Met Glu Ala Leu Thr Arg Lys Phe Arg Leu Ala Ala Asp 
            900                 905                 910         


Val Ser Leu Glu Ala Ile Ser Lys Arg Cys Pro Phe Thr Phe Thr Gly 
        915                 920                 925             


Ala Asp Phe Tyr Ala Leu Cys Ser Asp Ala Met Leu Asn Ala Met Thr 
    930                 935                 940                 


Arg Thr Ala Asn Glu Val Asp Ala Lys Ile Lys Leu Leu Asn Lys Asn 
945                 950                 955                 960 


Arg Glu Glu Ala Gly Glu Glu Pro Val Ser Ile Arg Trp Trp Phe Asp 
                965                 970                 975     


His Glu Ala Thr Lys Ser Asp Ile Glu Val Glu Val Ala Gln Gln Asp 
            980                 985                 990         


Phe Glu Lys Ala Lys Asp Glu Leu  Ser Pro Ser Val Ser  Ala Glu Glu 
        995                 1000                 1005             


Leu Gln  His Tyr Leu Lys Leu  Arg Gln Gln Phe Glu  Gly Gly Lys 
    1010                 1015                 1020             


Lys 
    


<210>  102
<211>  356
<212>  PRT
<213>  Yarrowia lipolytica CLIB122 (GenBank Accession No. CAG78389)


<220>
<221>  MISC_FEATURE
<222>  (1)..(356)
<223>  YlPex7p

<400>  102

Met Leu Gly Phe Lys Thr Gln Gly Phe Asn Gly Tyr Ala Ala Asn Tyr 
1               5                   10                  15      


Ser Pro Phe Phe Asn Asp Lys Ile Ala Val Gly Thr Ala Ala Asn Tyr 
            20                  25                  30          


Gly Leu Val Gly Asn Gly Lys Leu Phe Ile Leu Gly Ile Ser Pro Glu 
        35                  40                  45              


Gly Arg Met Val Cys Glu Gly Gln Phe Asp Thr Gln Asp Gly Ile Phe 
    50                  55                  60                  


Asp Val Ala Trp Ser Glu Gln His Glu Asn His Val Ala Thr Ala Cys 
65                  70                  75                  80  


Gly Asp Gly Ser Val Lys Leu Phe Asp Ile Lys Ala Gly Ala Phe Pro 
                85                  90                  95      


Leu Val Ser Phe Lys Glu His Thr Arg Glu Val Phe Ser Val Asn Trp 
            100                 105                 110         


Asn Met Ala Asn Lys Ala Leu Phe Cys Thr Ser Ser Trp Asp Ser Thr 
        115                 120                 125             


Ile Lys Ile Trp Thr Pro Glu Arg Thr Asn Ser Ile Met Thr Leu Gly 
    130                 135                 140                 


Gln Pro Ala Pro Ala Gln Gly Thr Asn Ala Ser Ala His Ile Gly Arg 
145                 150                 155                 160 


Gln Thr Ala Pro Asn Gln Ala Ala Ala Gln Glu Cys Ile Tyr Ser Ala 
                165                 170                 175     


Lys Phe Ser Pro His Thr Asp Ser Ile Ile Ala Ser Ala His Ser Thr 
            180                 185                 190         


Gly Met Val Lys Val Trp Asp Thr Arg Ala Pro Gln Pro Leu Gln Gln 
        195                 200                 205             


Gln Phe Ser Thr Gln Gln Thr Glu Ser Gly Gly Pro Pro Glu Val Leu 
    210                 215                 220                 


Ser Leu Asp Trp Asn Lys Tyr Arg Pro Thr Val Ile Ala Thr Gly Gly 
225                 230                 235                 240 


Val Asp Arg Ser Val Gln Val Tyr Asp Ile Arg Met Thr Gln Pro Ala 
                245                 250                 255     


Ala Asn Gln Pro Val Gln Pro Leu Ser Leu Ile Leu Gly His Arg Leu 
            260                 265                 270         


Pro Val Arg Gly Val Ser Trp Ser Pro His His Ala Asp Leu Leu Leu 
        275                 280                 285             


Ser Cys Ser Tyr Asp Met Thr Ala Arg Val Trp Arg Asp Ala Ser Thr 
    290                 295                 300                 


Gly Gly Asn Tyr Leu Ala Arg Gln Arg Gly Gly Thr Glu Val Lys Cys 
305                 310                 315                 320 


Met Asp Arg His Thr Glu Phe Val Ile Gly Gly Asp Trp Ser Leu Trp 
                325                 330                 335     


Gly Asp Pro Gly Trp Ile Thr Thr Val Gly Trp Asp Gln Met Val Tyr 
            340                 345                 350         


Val Trp His Ala 
        355     


<210>  103
<211>  671
<212>  PRT
<213>  Yarrowia lipolytica CLIB122 (GenBank Accession No. CAG80447)


<220>
<221>  MISC_FEATURE
<222>  (1)..(671)
<223>  YlPex8p

<400>  103

Met Asn Lys Tyr Leu Val Pro Pro Pro Gln Ala Asn Arg Thr Val Thr 
1               5                   10                  15      


Asn Leu Asp Leu Leu Ile Asn Asn Leu Arg Gly Ser Ser Thr Pro Gly 
            20                  25                  30          


Ala Ala Glu Val Asp Thr Arg Asp Ile Leu Gln Arg Ile Val Phe Ile 
        35                  40                  45              


Leu Pro Thr Ile Lys Asn Pro Leu Asn Leu Asp Leu Val Ile Lys Glu 
    50                  55                  60                  


Ile Ile Asn Ser Pro Arg Leu Leu Pro Pro Leu Ile Asp Leu His Asp 
65                  70                  75                  80  


Tyr Gln Gln Leu Thr Asp Ala Phe Arg Ala Thr Ile Lys Arg Lys Ala 
                85                  90                  95      


Leu Val Thr Asp Pro Thr Ile Ser Phe Glu Ala Trp Leu Glu Thr Cys 
            100                 105                 110         


Phe Gln Val Ile Thr Arg Phe Ala Gly Pro Gly Trp Lys Lys Leu Pro 
        115                 120                 125             


Leu Leu Ala Gly Leu Ile Leu Ala Asp Tyr Asp Ile Ser Ala Asp Gly 
    130                 135                 140                 


Pro Thr Leu Glu Arg Lys Pro Gly Phe Pro Ser Lys Leu Lys His Leu 
145                 150                 155                 160 


Leu Lys Arg Glu Phe Val Thr Thr Phe Asp Gln Cys Leu Ser Ile Asp 
                165                 170                 175     


Thr Arg Asn Arg Ser Asp Ala Thr Lys Trp Val Pro Val Leu Ala Cys 
            180                 185                 190         


Ile Ser Ile Ala Gln Val Tyr Ser Leu Leu Gly Asp Val Ala Ile Asn 
        195                 200                 205             


Tyr Arg Arg Phe Leu Gln Val Gly Leu Asp Leu Ile Phe Ser Asn Tyr 
    210                 215                 220                 


Gly Leu Glu Met Gly Thr Ala Leu Ala Arg Leu His Ala Glu Ser Gly 
225                 230                 235                 240 


Gly Asp Ala Thr Thr Ala Gly Gly Leu Ile Gly Lys Lys Leu Lys Glu 
                245                 250                 255     


Pro Val Val Ala Leu Leu Asn Thr Phe Ala His Ile Ala Ser Ser Cys 
            260                 265                 270         


Ile Val His Val Asp Ile Asp Tyr Ile Asp Arg Ile Gln Asn Lys Ile 
        275                 280                 285             


Ile Leu Val Cys Glu Asn Gln Ala Glu Thr Trp Arg Ile Leu Thr Ile 
    290                 295                 300                 


Glu Ser Pro Thr Val Met His His Gln Glu Ser Val Gln Tyr Leu Lys 
305                 310                 315                 320 


Trp Glu Leu Phe Thr Leu Cys Ile Ile Met Gln Gly Ile Ala Asn Met 
                325                 330                 335     


Leu Leu Thr Gln Lys Met Asn Gln Phe Met Tyr Leu Gln Leu Ala Tyr 
            340                 345                 350         


Lys Gln Leu Gln Ala Leu His Ser Ile Tyr Phe Ile Val Asp Gln Met 
        355                 360                 365             


Gly Ser Gln Phe Ala Ala Tyr Asp Tyr Val Phe Phe Ser Ala Ile Asp 
    370                 375                 380                 


Val Leu Leu Ser Glu Tyr Ala Pro Tyr Ile Lys Asn Arg Gly Thr Ile 
385                 390                 395                 400 


Pro Pro Asn Lys Glu Phe Val Ala Glu Arg Leu Ala Ala Asn Leu Ala 
                405                 410                 415     


Gly Thr Ser Asn Val Gly Ser His Leu Pro Ile Asp Arg Ser Arg Val 
            420                 425                 430         


Leu Phe Ala Leu Asn Tyr Tyr Glu Gln Leu Val Thr Val Cys His Asp 
        435                 440                 445             


Ser Cys Val Glu Thr Ile Ile Tyr Pro Met Ala Arg Ser Phe Leu Tyr 
    450                 455                 460                 


Pro Thr Ser Asp Ile Gln Gln Leu Lys Pro Leu Val Glu Ala Ala His 
465                 470                 475                 480 


Ser Val Ile Leu Ala Gly Leu Ala Val Pro Thr Asn Ala Val Val Asn 
                485                 490                 495     


Ala Lys Leu Ile Pro Glu Tyr Met Gly Gly Val Leu Pro Leu Phe Pro 
            500                 505                 510         


Gly Val Phe Ser Trp Asn Gln Phe Val Leu Ala Ile Gln Ser Ile Val 
        515                 520                 525             


Asn Thr Val Ser Pro Pro Ser Glu Val Phe Lys Thr Asn Gln Lys Leu 
    530                 535                 540                 


Phe Arg Leu Val Leu Asp Ser Leu Met Lys Lys Cys Arg Asp Thr Pro 
545                 550                 555                 560 


Val Gly Ile Pro Val Pro His Ser Val Thr Val Ser Gln Glu Gln Glu 
                565                 570                 575     


Asp Ile Pro Pro Thr Gln Arg Ala Val Val Met Leu Ala Leu Ile Asn 
            580                 585                 590         


Ser Leu Pro Tyr Val Asp Ile Arg Ser Phe Glu Leu Trp Leu Gln Glu 
        595                 600                 605             


Thr Trp Asn Met Ile Glu Ala Thr Pro Met Leu Ala Glu Asn Ala Pro 
    610                 615                 620                 


Asn Lys Glu Leu Ala His Ala Glu His Glu Phe Leu Val Leu Glu Met 
625                 630                 635                 640 


Trp Lys Met Ile Ser Gly Asn Ile Asp Gln Arg Leu Asn Asp Val Ala 
                645                 650                 655     


Ile Arg Trp Trp Tyr Lys Lys Asn Ala Arg Val His Gly Thr Leu 
            660                 665                 670     


<210>  104
<211>  377
<212>  PRT
<213>  Yarrowia lipolytica CLIB122 (GenBank Accession No. CAG81606)


<220>
<221>  MISC_FEATURE
<222>  (1)..(377)
<223>  YlPex10p

<400>  104

Met Trp Gly Ser Ser His Ala Phe Ala Gly Glu Ser Asp Leu Thr Leu 
1               5                   10                  15      


Gln Leu His Thr Arg Ser Asn Met Ser Asp Asn Thr Thr Ile Lys Lys 
            20                  25                  30          


Pro Ile Arg Pro Lys Pro Ile Arg Thr Glu Arg Leu Pro Tyr Ala Gly 
        35                  40                  45              


Ala Ala Glu Ile Ile Arg Ala Asn Gln Lys Asp His Tyr Phe Glu Ser 
    50                  55                  60                  


Val Leu Glu Gln His Leu Val Thr Phe Leu Gln Lys Trp Lys Gly Val 
65                  70                  75                  80  


Arg Phe Ile His Gln Tyr Lys Glu Glu Leu Glu Thr Ala Ser Lys Phe 
                85                  90                  95      


Ala Tyr Leu Gly Leu Cys Thr Leu Val Gly Ser Lys Thr Leu Gly Glu 
            100                 105                 110         


Glu Tyr Thr Asn Leu Met Tyr Thr Ile Arg Asp Arg Thr Ala Leu Pro 
        115                 120                 125             


Gly Val Val Arg Arg Phe Gly Tyr Val Leu Ser Asn Thr Leu Phe Pro 
    130                 135                 140                 


Tyr Leu Phe Val Arg Tyr Met Gly Lys Leu Arg Ala Lys Leu Met Arg 
145                 150                 155                 160 


Glu Tyr Pro His Leu Val Glu Tyr Asp Glu Asp Glu Pro Val Pro Ser 
                165                 170                 175     


Pro Glu Thr Trp Lys Glu Arg Val Ile Lys Thr Phe Val Asn Lys Phe 
            180                 185                 190         


Asp Lys Phe Thr Ala Leu Glu Gly Phe Thr Ala Ile His Leu Ala Ile 
        195                 200                 205             


Phe Tyr Val Tyr Gly Ser Tyr Tyr Gln Leu Ser Lys Arg Ile Trp Gly 
    210                 215                 220                 


Met Arg Tyr Val Phe Gly His Arg Leu Asp Lys Asn Glu Pro Arg Ile 
225                 230                 235                 240 


Gly Tyr Glu Met Leu Gly Leu Leu Ile Phe Ala Arg Phe Ala Thr Ser 
                245                 250                 255     


Phe Val Gln Thr Gly Arg Glu Tyr Leu Gly Ala Leu Leu Glu Lys Ser 
            260                 265                 270         


Val Glu Lys Glu Ala Gly Glu Lys Glu Asp Glu Lys Glu Ala Val Val 
        275                 280                 285             


Pro Lys Lys Lys Ser Ser Ile Pro Phe Ile Glu Asp Thr Glu Gly Glu 
    290                 295                 300                 


Thr Glu Asp Lys Ile Asp Leu Glu Asp Pro Arg Gln Leu Lys Phe Ile 
305                 310                 315                 320 


Pro Glu Ala Ser Arg Ala Cys Thr Leu Cys Leu Ser Tyr Ile Ser Ala 
                325                 330                 335     


Pro Ala Cys Thr Pro Cys Gly His Phe Phe Cys Trp Asp Cys Ile Ser 
            340                 345                 350         


Glu Trp Val Arg Glu Lys Pro Glu Cys Pro Leu Cys Arg Gln Gly Val 
        355                 360                 365             


Arg Glu Gln Asn Leu Leu Pro Ile Arg 
    370                 375         


<210>  105
<211>  408
<212>  PRT
<213>  Yarrowia lipolytica CLIB122 (GenBank Accession No. CAG81532)


<220>
<221>  MISC_FEATURE
<222>  (1)..(408)
<223>  YlPex12p

<400>  105

Met Asp Tyr Phe Ser Ser Leu Asn Ala Ser Gln Leu Asp Pro Asp Val 
1               5                   10                  15      


Pro Thr Leu Phe Glu Leu Leu Ser Ala Lys Gln Leu Glu Gly Leu Ile 
            20                  25                  30          


Ala Pro Ser Val Arg Tyr Ile Leu Ala Phe Tyr Ala Gln Arg His Pro 
        35                  40                  45              


Arg Tyr Leu Leu Arg Ile Val Asn Arg Tyr Asp Glu Leu Tyr Ala Leu 
    50                  55                  60                  


Phe Met Gly Leu Val Glu Tyr Tyr Asn Leu Lys Thr Trp Asn Ala Ser 
65                  70                  75                  80  


Phe Thr Glu Lys Phe Tyr Gly Leu Lys Arg Thr Gln Ile Leu Thr Asn 
                85                  90                  95      


Pro Ala Leu Arg Thr Arg Gln Ala Val Pro Asp Leu Val Glu Ala Glu 
            100                 105                 110         


Lys Arg Leu Ser Lys Lys Lys Ile Trp Gly Ser Leu Phe Phe Leu Ile 
        115                 120                 125             


Val Val Pro Tyr Val Lys Glu Lys Leu Asp Ala Arg Tyr Glu Arg Leu 
    130                 135                 140                 


Lys Gly Arg Tyr Leu Ala Arg Asp Ile Asn Glu Glu Arg Ile Glu Ile 
145                 150                 155                 160 


Lys Arg Thr Gly Thr Ala Gln Gln Ile Ala Val Phe Glu Phe Asp Tyr 
                165                 170                 175     


Trp Leu Leu Lys Leu Tyr Pro Ile Val Thr Met Gly Cys Thr Thr Ala 
            180                 185                 190         


Thr Leu Ala Phe His Met Leu Phe Leu Phe Ser Val Thr Arg Ala Tyr 
        195                 200                 205             


Ser Ile Asp Asp Phe Leu Leu Asn Ile Gln Phe Ser Arg Met Thr Arg 
    210                 215                 220                 


Tyr Asp Tyr Gln Met Glu Thr Gln Arg Asp Ser Arg Asn Ala Ala Asn 
225                 230                 235                 240 


Val Ala His Thr Met Lys Ser Ile Ser Glu Tyr Pro Val Ala Glu Arg 
                245                 250                 255     


Val Met Leu Leu Leu Thr Thr Lys Ala Gly Ala Asn Ala Met Arg Ser 
            260                 265                 270         


Ala Ala Leu Ser Gly Leu Ser Tyr Val Leu Pro Thr Ser Ile Phe Ala 
        275                 280                 285             


Leu Lys Phe Leu Glu Trp Trp Tyr Ala Ser Asp Phe Ala Arg Gln Leu 
    290                 295                 300                 


Asn Gln Lys Arg Arg Gly Asp Leu Glu Asp Asn Leu Pro Val Pro Asp 
305                 310                 315                 320 


Lys Val Lys Gly Ala Asp Lys Leu Ala Glu Ser Val Ala Lys Trp Lys 
                325                 330                 335     


Glu Asp Thr Ser Lys Cys Pro Leu Cys Ser Lys Glu Leu Val Asn Pro 
            340                 345                 350         


Thr Val Ile Glu Ser Gly Tyr Val Phe Cys Tyr Thr Cys Ile Tyr Arg 
        355                 360                 365             


His Leu Glu Asp Gly Asp Glu Glu Thr Gly Gly Arg Cys Pro Val Thr 
    370                 375                 380                 


Gly Gln Lys Leu Leu Gly Cys Arg Trp Gln Asp Asp Val Trp Gln Val 
385                 390                 395                 400 


Thr Gly Leu Arg Arg Leu Met Val 
                405             


<210>  106
<211>  412
<212>  PRT
<213>  Yarrowia lipolytica CLIB122 (GenBank Accession No. CAG81789)


<220>
<221>  MISC_FEATURE
<222>  (1)..(412)
<223>  YlPex13p

<400>  106

Met Ser Val Pro Arg Pro Lys Pro Trp Glu Gly Ala Ser Gly Ser Ser 
1               5                   10                  15      


Ala Ala Thr Ala Thr Pro Ala Ala Thr Ala Thr Pro Ala Ser Thr Asp 
            20                  25                  30          


Ala Val Ser Ser Ser Ala Gly Ser Ala Thr Gly Ala Pro Glu Leu Pro 
        35                  40                  45              


Ser Arg Pro Ser Ala Met Gly Ser Thr Ser Asn Ala Leu Ser Ser Pro 
    50                  55                  60                  


Met Gly Ser Ser Met Asn Ser Gly Tyr Gly Gly Met Asn Ser Gly Tyr 
65                  70                  75                  80  


Gly Gly Met Gly Ser Ser Tyr Gly Ser Gly Tyr Gly Ser Ser Tyr Gly 
                85                  90                  95      


Met Gly Ser Ser Tyr Gly Ser Gly Tyr Gly Ser Gly Leu Gly Gly Tyr 
            100                 105                 110         


Gly Ser Tyr Gly Gly Met Gly Gly Met Gly Gly Met Tyr Gly Ser Arg 
        115                 120                 125             


Tyr Gly Gly Tyr Gly Ser Tyr Gly Gly Met Gly Gly Tyr Gly Gly Tyr 
    130                 135                 140                 


Gly Gly Met Gly Gly Gly Pro Met Gly Gln Asn Gly Leu Ala Gly Gly 
145                 150                 155                 160 


Thr Gln Ala Thr Phe Gln Leu Ile Glu Ser Ile Val Gly Ala Val Gly 
                165                 170                 175     


Gly Phe Ala Gln Met Leu Glu Ser Thr Tyr Met Ala Thr Gln Ser Ser 
            180                 185                 190         


Phe Phe Ala Met Val Ser Val Ala Glu Gln Phe Gly Asn Leu Lys Asn 
        195                 200                 205             


Thr Leu Gly Ser Leu Leu Gly Ile Tyr Ala Ile Met Arg Trp Ala Arg 
    210                 215                 220                 


Arg Leu Val Ala Lys Leu Ser Gly Gln Pro Val Thr Gly Ala Asn Gly 
225                 230                 235                 240 


Ile Thr Pro Ala Gly Phe Ala Lys Phe Glu Ala Thr Gly Gly Ala Ala 
                245                 250                 255     


Gly Pro Gly Arg Gly Pro Arg Pro Ser Tyr Lys Pro Leu Leu Phe Phe 
            260                 265                 270         


Leu Thr Ala Val Phe Gly Leu Pro Tyr Leu Leu Gly Arg Leu Ile Lys 
        275                 280                 285             


Ala Leu Ala Ala Lys Gln Glu Gly Met Tyr Asp Glu His Gly Asn Leu 
    290                 295                 300                 


Leu Pro Gly Ala Gln Met Gly Met Gly Gly Pro Gly Met Glu Gly Gly 
305                 310                 315                 320 


Ala Glu Ile Asp Pro Ser Lys Leu Glu Phe Cys Arg Ala Asn Phe Asp 
                325                 330                 335     


Phe Val Pro Glu Asn Pro Gln Leu Glu Leu Glu Leu Arg Lys Gly Asp 
            340                 345                 350         


Leu Val Ala Val Leu Ala Lys Thr Asp Pro Met Gly Asn Pro Ser Gln 
        355                 360                 365             


Trp Trp Arg Val Arg Thr Arg Asp Gly Arg Ser Gly Tyr Val Pro Ala 
    370                 375                 380                 


Asn Tyr Leu Glu Val Ile Pro Arg Pro Ala Val Glu Ala Pro Lys Lys 
385                 390                 395                 400 


Val Glu Glu Ile Gly Ala Ser Ala Val Pro Val Asn 
                405                 410         


<210>  107
<211>  380
<212>  PRT
<213>  Yarrowia lipolytica CLIB122 (GenBank Accession No. CAG79323)


<220>
<221>  MISC_FEATURE
<222>  (1)..(380)
<223>  YlPex14p

<400>  107

Met Ile Pro Ser Cys Leu Ser Thr Gln His Met Ala Pro Arg Glu Asp 
1               5                   10                  15      


Leu Val Gln Ser Ala Val Ala Phe Leu Asn Asp Pro Gln Ala Ala Thr 
            20                  25                  30          


Ala Pro Leu Ala Lys Arg Ile Glu Phe Leu Glu Ser Lys Asp Met Thr 
        35                  40                  45              


Pro Glu Glu Ile Glu Glu Ala Leu Lys Arg Ala Gly Ser Gly Ser Ala 
    50                  55                  60                  


Gln Ser His Pro Gly Ser Val Val Ser His Gly Gly Ala Ala Pro Thr 
65                  70                  75                  80  


Val Pro Ala Ser Tyr Ala Phe Gln Ser Ala Pro Pro Leu Pro Glu Arg 
                85                  90                  95      


Asp Trp Lys Asp Val Phe Ile Met Ala Thr Val Thr Val Gly Val Gly 
            100                 105                 110         


Phe Gly Leu Tyr Thr Val Ala Lys Arg Tyr Leu Met Pro Leu Ile Leu 
        115                 120                 125             


Pro Pro Thr Pro Pro Ser Leu Glu Ala Asp Lys Glu Ala Leu Glu Ala 
    130                 135                 140                 


Glu Phe Ala Arg Val Gln Gly Leu Leu Asp Gln Val Gln Gln Asp Thr 
145                 150                 155                 160 


Glu Glu Val Lys Asn Ser Gln Val Glu Val Ala Lys Arg Val Thr Asp 
                165                 170                 175     


Ala Leu Lys Gly Val Glu Glu Thr Ile Asp Gln Leu Lys Ser Gln Thr 
            180                 185                 190         


Lys Lys Arg Asp Asp Glu Met Lys Leu Val Thr Ala Glu Val Glu Arg 
        195                 200                 205             


Ile Arg Asp Arg Leu Pro Lys Asn Ile Asp Lys Leu Lys Asp Ser Gln 
    210                 215                 220                 


Glu Gln Gly Leu Ala Asp Ile Gln Ser Glu Leu Lys Ser Leu Lys Gln 
225                 230                 235                 240 


Leu Leu Ser Thr Arg Thr Ala Ala Ser Ser Gly Pro Lys Leu Pro Pro 
                245                 250                 255     


Ile Pro Pro Pro Ser Ser Tyr Leu Thr Arg Lys Ala Ser Pro Ala Val 
            260                 265                 270         


Pro Ala Ala Ala Pro Ala Pro Val Thr Pro Gly Ser Pro Val His Asn 
        275                 280                 285             


Val Ser Ser Ser Ser Thr Val Pro Ala Asp Arg Asp Asp Phe Ile Pro 
    290                 295                 300                 


Thr Pro Ala Gly Ala Val Pro Met Ile Pro Gln Pro Ala Ser Met Ser 
305                 310                 315                 320 


Ser Ser Ser Thr Ser Thr Val Pro Asn Ser Ala Ile Ser Ser Ala Pro 
                325                 330                 335     


Ser Pro Ile Gln Glu Pro Glu Pro Phe Val Pro Glu Pro Gly Asn Ser 
            340                 345                 350         


Ala Val Lys Lys Pro Ala Pro Lys Ala Ser Ile Pro Ala Trp Gln Leu 
        355                 360                 365             


Ala Ala Leu Glu Lys Glu Lys Glu Lys Glu Lys Glu 
    370                 375                 380 


<210>  108
<211>  391
<212>  PRT
<213>  Yarrowia lipolytica CLIB122 (GenBank Accession No. CAG79622)


<220>
<221>  MISC_FEATURE
<222>  (1)..(391)
<223>  YlPex16p

<400>  108

Met Thr Asp Lys Leu Val Lys Val Met Gln Lys Lys Lys Ser Ala Pro 
1               5                   10                  15      


Gln Thr Trp Leu Asp Ser Tyr Asp Lys Phe Leu Val Arg Asn Ala Ala 
            20                  25                  30          


Ser Ile Gly Ser Ile Glu Ser Thr Leu Arg Thr Val Ser Tyr Val Leu 
        35                  40                  45              


Pro Gly Arg Phe Asn Asp Val Glu Ile Ala Thr Glu Thr Leu Tyr Ala 
    50                  55                  60                  


Val Leu Asn Val Leu Gly Leu Tyr His Asp Thr Ile Ile Ala Arg Ala 
65                  70                  75                  80  


Val Ala Ala Ser Pro Asn Ala Ala Ala Val Tyr Arg Pro Ser Pro His 
                85                  90                  95      


Asn Arg Tyr Thr Asp Trp Phe Ile Lys Asn Arg Lys Gly Tyr Lys Tyr 
            100                 105                 110         


Ala Ser Arg Ala Val Thr Phe Val Lys Phe Gly Glu Leu Val Ala Glu 
        115                 120                 125             


Met Val Ala Lys Lys Asn Gly Gly Glu Met Ala Arg Trp Lys Cys Ile 
    130                 135                 140                 


Ile Gly Ile Glu Gly Ile Lys Ala Gly Leu Arg Ile Tyr Met Leu Gly 
145                 150                 155                 160 


Ser Thr Leu Tyr Gln Pro Leu Cys Thr Thr Pro Tyr Pro Asp Arg Glu 
                165                 170                 175     


Val Thr Gly Glu Leu Leu Glu Thr Ile Cys Arg Asp Glu Gly Glu Leu 
            180                 185                 190         


Asp Ile Glu Lys Gly Leu Met Asp Pro Gln Trp Lys Met Pro Arg Thr 
        195                 200                 205             


Gly Arg Thr Ile Pro Glu Ile Ala Pro Thr Asn Val Glu Gly Tyr Leu 
    210                 215                 220                 


Leu Thr Lys Val Leu Arg Ser Glu Asp Val Asp Arg Pro Tyr Asn Leu 
225                 230                 235                 240 


Leu Ser Arg Leu Asp Asn Trp Gly Val Val Ala Glu Leu Leu Ser Ile 
                245                 250                 255     


Leu Arg Pro Leu Ile Tyr Ala Cys Leu Leu Phe Arg Gln His Val Asn 
            260                 265                 270         


Lys Thr Val Pro Ala Ser Thr Lys Ser Lys Phe Pro Phe Leu Asn Ser 
        275                 280                 285             


Pro Trp Ala Pro Trp Ile Ile Gly Leu Val Ile Glu Ala Leu Ser Arg 
    290                 295                 300                 


Lys Met Met Gly Ser Trp Leu Leu Arg Gln Arg Gln Ser Gly Lys Thr 
305                 310                 315                 320 


Pro Thr Ala Leu Asp Gln Met Glu Val Lys Gly Arg Thr Asn Leu Leu 
                325                 330                 335     


Gly Trp Trp Leu Phe Arg Gly Glu Phe Tyr Gln Ala Tyr Thr Arg Pro 
            340                 345                 350         


Leu Leu Tyr Ser Ile Val Ala Arg Leu Glu Lys Ile Pro Gly Leu Gly 
        355                 360                 365             


Leu Phe Gly Ala Leu Ile Ser Asp Tyr Leu Tyr Leu Phe Asp Arg Tyr 
    370                 375                 380                 


Tyr Phe Thr Ala Ser Thr Leu 
385                 390     


<210>  109
<211>  225
<212>  PRT
<213>  Yarrowia lipolytica CLIB122 (GenBank Accession No. CAG84025)


<220>
<221>  MISC_FEATURE
<222>  (1)..(225)
<223>  YlPex17p

<400>  109

Met Ser Ala Phe Pro Glu Pro Ser Ser Phe Glu Ile Glu Phe Ala Lys 
1               5                   10                  15      


Gln Met Asn Arg Pro Arg Thr Val Gln Phe Lys Gln Leu Val Ala Val 
            20                  25                  30          


Leu Tyr Ile Phe Gly Gly Thr Ser Ala Leu Ile Tyr Ile Ile Ser Lys 
        35                  40                  45              


Thr Ile Leu Asn Pro Leu Phe Glu Glu Leu Thr Phe Ala Arg Ser Glu 
    50                  55                  60                  


Tyr Ala Ile His Ala Arg Arg Leu Met Glu Gln Leu Asn Ala Lys Leu 
65                  70                  75                  80  


Ser Ser Met Ala Ser Tyr Ile Pro Pro Val Arg Ala Leu Gln Gly Gln 
                85                  90                  95      


Arg Phe Val Asp Ala Gln Thr Gln Thr Glu Asp Glu Glu Gly Glu Asp 
            100                 105                 110         


Ile Pro Asn Pro Ser Leu Gly Lys Ser Ser His Val Ser Phe Gly Glu 
        115                 120                 125             


Ser Pro Met Gln Leu Lys Leu Ala Glu Lys Glu Lys Gln Gln Lys Leu 
    130                 135                 140                 


Ile Asp Asp Ser Val Asp Asn Leu Glu Arg Leu Ala Asp Ser Leu Lys 
145                 150                 155                 160 


His Ala Gly Glu Val Ser Asp Leu Ser Ala Leu Ser Gly Phe Lys Tyr 
                165                 170                 175     


Gln Val Glu Glu Leu Thr Asn Tyr Ser Asp Gln Leu Ala Met Ser Gly 
            180                 185                 190         


Tyr Ser Met Met Lys Ser Gly Leu Pro Gly His Glu Thr Ala Met Ser 
        195                 200                 205             


Glu Thr Lys Lys Glu Ile Arg Ser Leu Lys Gly Ser Val Leu Ser Val 
    210                 215                 220                 


Arg 
225 


<210>  110
<211>  324
<212>  PRT
<213>  Yarrowia lipolytica (GenBank Accession No. AAK84827)


<220>
<221>  MISC_FEATURE
<222>  (1)..(324)
<223>  YlPex19p

<400>  110

Met Ser His Glu Glu Asp Leu Asp Asp Leu Asp Asp Phe Leu Asp Glu 
1               5                   10                  15      


Phe Asp Glu Gln Val Leu Ser Lys Pro Pro Gly Ala Gln Lys Asp Ala 
            20                  25                  30          


Thr Pro Thr Thr Ser Thr Ala Pro Thr Thr Ala Glu Ala Lys Pro Asp 
        35                  40                  45              


Ala Thr Lys Lys Ser Thr Glu Thr Ser Gly Thr Asp Ser Lys Thr Glu 
    50                  55                  60                  


Gly Ala Asp Thr Ala Asp Lys Asn Ala Ala Thr Asp Ser Ala Glu Ala 
65                  70                  75                  80  


Gly Ala Glu Lys Val Ser Leu Pro Asn Leu Glu Asp Gln Leu Ala Gly 
                85                  90                  95      


Leu Lys Met Asp Asp Phe Leu Lys Asp Ile Glu Ala Asp Pro Glu Ser 
            100                 105                 110         


Lys Ala Gln Phe Glu Ser Leu Leu Lys Glu Ile Asn Asn Val Thr Ser 
        115                 120                 125             


Ala Thr Ala Ser Glu Lys Ala Gln Gln Pro Lys Ser Phe Lys Glu Thr 
    130                 135                 140                 


Ile Ser Ala Thr Ala Asp Arg Leu Asn Gln Ser Asn Gln Glu Met Gly 
145                 150                 155                 160 


Asp Met Pro Leu Gly Asp Asp Met Leu Ala Gly Leu Met Glu Gln Leu 
                165                 170                 175     


Ser Gly Ala Gly Gly Phe Gly Glu Gly Gly Glu Gly Asp Phe Gly Asp 
            180                 185                 190         


Met Leu Gly Gly Ile Met Arg Gln Leu Ala Ser Lys Glu Val Leu Tyr 
        195                 200                 205             


Gln Pro Leu Lys Glu Met His Asp Asn Tyr Pro Lys Trp Trp Asp Glu 
    210                 215                 220                 


His Gly Ser Lys Val Thr Glu Glu Lys Glu Arg Asp Arg Leu Lys Leu 
225                 230                 235                 240 


Gln Gln Asp Ile Val Gly Lys Ile Cys Ala Lys Phe Glu Asp Pro Ser 
                245                 250                 255     


Tyr Ser Asp Asp Ser Glu Ala Asp Arg Ala Val Ile Thr Gln Leu Met 
            260                 265                 270         


Asp Glu Met Gln Glu Thr Gly Ala Pro Pro Asp Glu Ile Met Ser Asn 
        275                 280                 285             


Val Ala Asp Gly Ser Ile Pro Gly Gly Leu Asp Gly Leu Gly Leu Gly 
    290                 295                 300                 


Gly Leu Gly Gly Gly Lys Met Pro Glu Met Pro Glu Asn Met Pro Glu 
305                 310                 315                 320 


Cys Asn Gln Gln 
                


<210>  111
<211>  417
<212>  PRT
<213>  Yarrowia lipolytica CLIB122 (GenBank Accession No. CAG79226)


<220>
<221>  MISC_FEATURE
<222>  (1)..(417)
<223>  YlPex20p

<400>  111

Met Ala Ser Cys Gly Pro Ser Asn Ala Leu Gln Asn Leu Ser Lys His 
1               5                   10                  15      


Ala Ser Ala Asp Arg Ser Leu Gln His Asp Arg Met Ala Pro Gly Gly 
            20                  25                  30          


Ala Pro Gly Ala Gln Arg Gln Gln Phe Arg Ser Gln Thr Gln Gly Gly 
        35                  40                  45              


Gln Leu Asn Asn Glu Phe Gln Gln Phe Ala Gln Ala Gly Pro Ala His 
    50                  55                  60                  


Asn Ser Phe Glu Gln Ser Gln Met Gly Pro His Phe Gly Gln Gln His 
65                  70                  75                  80  


Phe Gly Gln Pro His Gln Pro Gln Met Gly Gln His Ala Pro Met Ala 
                85                  90                  95      


His Gly Gln Gln Ser Asp Trp Ala Gln Ser Phe Ser Gln Leu Asn Leu 
            100                 105                 110         


Gly Pro Gln Thr Gly Pro Gln His Thr Gln Gln Ser Asn Trp Gly Gln 
        115                 120                 125             


Asp Phe Met Arg Gln Ser Pro Gln Ser His Gln Val Gln Pro Gln Met 
    130                 135                 140                 


Ala Asn Gly Val Met Gly Ser Met Ser Gly Met Ser Ser Phe Gly Pro 
145                 150                 155                 160 


Met Tyr Ser Asn Ser Gln Leu Met Asn Ser Thr Tyr Gly Leu Gln Thr 
                165                 170                 175     


Glu His Gln Gln Thr His Lys Thr Glu Thr Lys Ser Ser Gln Asp Ala 
            180                 185                 190         


Ala Phe Glu Ala Ala Phe Gly Ala Val Glu Glu Ser Ile Thr Lys Thr 
        195                 200                 205             


Ser Asp Lys Gly Lys Glu Val Glu Lys Asp Pro Met Glu Gln Thr Tyr 
    210                 215                 220                 


Arg Tyr Asp Gln Ala Asp Ala Leu Asn Arg Gln Ala Glu His Ile Ser 
225                 230                 235                 240 


Asp Asn Ile Ser Arg Glu Glu Val Asp Ile Lys Thr Asp Glu Asn Gly 
                245                 250                 255     


Glu Phe Ala Ser Ile Ala Arg Gln Ile Ala Ser Ser Leu Glu Glu Ala 
            260                 265                 270         


Asp Lys Ser Lys Phe Glu Lys Ser Thr Phe Met Asn Leu Met Arg Arg 
        275                 280                 285             


Ile Gly Asn His Glu Val Thr Leu Asp Gly Asp Lys Leu Val Asn Lys 
    290                 295                 300                 


Glu Gly Glu Asp Ile Arg Glu Glu Val Arg Asp Glu Leu Leu Arg Glu 
305                 310                 315                 320 


Gly Ala Ser Gln Glu Asn Gly Phe Gln Ser Glu Ala Gln Gln Thr Ala 
                325                 330                 335     


Pro Leu Pro Val His His Glu Ala Pro Pro Pro Glu Gln Ile His Pro 
            340                 345                 350         


His Thr Glu Thr Gly Asp Lys Gln Leu Glu Asp Pro Met Val Tyr Ile 
        355                 360                 365             


Glu Gln Glu Ala Ala Arg Arg Ala Ala Glu Ser Gly Arg Thr Val Glu 
    370                 375                 380                 


Glu Glu Lys Leu Asn Phe Tyr Ser Pro Phe Glu Tyr Ala Gln Lys Leu 
385                 390                 395                 400 


Gly Pro Gln Gly Val Ala Lys Gln Ser Asn Trp Glu Glu Asp Tyr Asp 
                405                 410                 415     


Phe 
    


<210>  112
<211>  195
<212>  PRT
<213>  Yarrowia lipolytica CLIB122 (GenBank Accession No. CAG77876)


<220>
<221>  MISC_FEATURE
<222>  (1)..(195)
<223>  YlPex22p

<400>  112

Val Pro Arg Cys Thr Ser His Pro Cys Asn Leu Thr Leu His Leu Pro 
1               5                   10                  15      


Val Thr Thr Met Ala Pro Arg Lys Thr Arg Leu Pro Ala Val Ile Gly 
            20                  25                  30          


Ala Ala Ala Ala Ala Ala Ala Val Ala Tyr Leu Val Tyr Ser Phe Val 
        35                  40                  45              


Ala Lys Ser Asn Ser Asp Gln Asp Thr Phe Asp Ser Ser Val Gln Ser 
    50                  55                  60                  


Ser Ser Lys Ser Ser Thr Lys Ser Pro Lys Ser Thr Ala Thr Asn Ser 
65                  70                  75                  80  


Lys Ile Thr Val Val Val Ser Gln Glu Leu Val Gln Ser Gln Leu Val 
                85                  90                  95      


Asp Phe Lys His Leu Met Ser Val His Pro Asn Leu Val Val Ile Val 
            100                 105                 110         


Pro Pro Met Val Ala Asn Lys Phe His Arg Ala Leu Lys Ser Ser Val 
        115                 120                 125             


Gly His Asp His Gly Val Lys Val Ile Arg Cys Asp Thr Asp Val Gly 
    130                 135                 140                 


Val Ile His Val Ile Lys His Ile Arg Pro Asp Leu Ala Leu Ile Ala 
145                 150                 155                 160 


Asp Gly Val Gly Asp Asn Ile Gln Gly Glu Ile Lys Arg Phe Val Gly 
                165                 170                 175     


Ser Ser Glu Ala Leu Ser Gly Asp Val Asn Leu Ala Ala Glu Arg Leu 
            180                 185                 190         


Thr Gly Leu 
        195 


<210>  113
<211>  386
<212>  PRT
<213>  Yarrowia lipolytica CLIB122 (GenBank Accession No. NC_006072, antisense translation of nucleotides 117230-118387)


<220>
<221>  MISC_FEATURE
<222>  (1)..(386)
<223>  YlPex26p

<400>  113

Met Pro Pro Ala Met Pro Gln Met Thr Thr Ser Thr Leu Leu Thr Asp 
1               5                   10                  15      


Ser Val Thr Ser Ala Val Asn Gln Ala Ala Thr Pro Lys Val Asp Gln 
            20                  25                  30          


Met Tyr Gln Thr Phe Gly Glu Ser Ala Arg Glu Phe Val Asn Lys Asn 
        35                  40                  45              


Phe Tyr Asn Ser Tyr Glu Leu Ile Arg Pro Phe Phe Asp Glu Ile Thr 
    50                  55                  60                  


Ala Lys Gly Ala Gln Gln Asn Gly Ser Thr Val Leu Asp Ala Glu Asn 
65                  70                  75                  80  


Pro His Asn Ile Pro Leu Ser Leu Trp Ile Lys Val Trp Ser Leu Tyr 
                85                  90                  95      


Leu Ala Ile Leu Asp Ala Ser Cys Lys Gln Ala Gly Glu Ala Leu Leu 
            100                 105                 110         


Asn Ser Thr Gly Asp Leu Ser Gly Ser Asp Ser Gly Glu Trp Asn Gln 
        115                 120                 125             


Thr Arg Lys Leu Leu Ala Arg Lys Leu Thr Ser Gly Ser Val Trp Asp 
    130                 135                 140                 


Glu Leu Val Thr Ala Ser Gly Gly Thr Gly Asn Ile His Pro Thr Ile 
145                 150                 155                 160 


Leu Ala Leu Leu Ala Ser Leu Ser Ile Arg His Asp Thr Asp Ala Lys 
                165                 170                 175     


Leu Met Ala Asp Asn Leu Glu Lys Phe Ile Val Thr Tyr Asn Asp Asn 
            180                 185                 190         


Gly Ser Asp Asp Val Lys Thr Lys Thr Ala Phe Tyr Lys Val Leu Asp 
        195                 200                 205             


Leu Tyr Leu Leu Arg Val Leu Pro Asp Leu Gly Gln Trp Asp Val Ala 
    210                 215                 220                 


His Ser Phe Val Asn Asn Thr Asn Leu Phe Ser His Glu Gln Lys Lys 
225                 230                 235                 240 


Glu Met Thr His Lys Leu Asp Gln Ser Gln Lys His Ala Glu Gln Glu 
                245                 250                 255     


His Lys Arg Leu Leu Glu Glu Ala Gln Glu Lys Glu Lys Ser Asp Ala 
            260                 265                 270         


Lys Glu Lys Glu Arg Glu Glu Arg Val Ser Arg Asp Thr Gln Ser Arg 
        275                 280                 285             


Glu Ile Lys Ser Pro Ile Val Asp Ser Ser Thr Ser Ser Arg Asp Val 
    290                 295                 300                 


Thr Arg Asp Thr Thr Arg Glu Leu Ser Lys Ser Ser Arg Gln Pro Arg 
305                 310                 315                 320 


Thr Leu Ser Gln Ile Ile Ser Thr Ser Leu Lys Ser Gln Phe Asp Gly 
                325                 330                 335     


Asn Ala Ile Phe Arg Thr Leu Ala Leu Ile Val Ile Val Ser Leu Ser 
            340                 345                 350         


Ala Ala Asn Pro Leu Ile Arg Lys Arg Val Val Asp Thr Leu Lys Met 
        355                 360                 365             


Leu Trp Ile Lys Ile Leu Gln Thr Leu Ser Met Gly Phe Lys Val Ser 
    370                 375                 380                 


Tyr Leu 
385     


<210>  114
<211>  3387
<212>  DNA
<213>  Yarrowia lipolytica (GenBank Accession No. AB036770)

<400>  114
ggtaccatca agggtaaaat caaggctatc atcaagggcc atatatcgca agtttggggg     60

aagataatat gttcatagtg aatcgggttg tggatttcct catctaacgg cattataact    120

agtcctggag ggtctttttt atggataacc tccatgtacg atgtatccaa gatctccacg    180

tactgtgttc tgtttcctaa gtaataccca acaacctctc caacaaacac ttgggaagat    240

gcacttgtgc tgagatgtca agatgttaga gagtagagac agtagcaagc gtaaaaggcg    300

gccgaggcca ccgagagaac agcgtagcag ggcgcgtagt caccacaggg gacgcagaac    360

caaacaaatg acgaagaaga accacaagga gacgttttca aaggcaatgc aaacgaagag    420

ggcaatggaa ggattgagat tagagaactg gagactggag tggcgttttc ccgatgaacg    480

aacaaacacg cgaagctatg tggaccaaca tacaacacgg actgaaccag gtttttttat    540

gattttttta ctggaaatag gtacgtgcca agttggacca tgacactaaa cgtgtttaat    600

tagtaatatt cgtgtaagcg tacattcatt tcaaaggtta ttctttcacg gcaaagttat    660

aattaaatga atgtatatgc agaaaaaaaa aaaaaaagta ctgtactgga tggagagaat    720

attaataaat aattgttacc caactacatc ttgtcgattg aaagagaccc ctaagacaga    780

taggatatct gcaacccgag gaatgaaccc cccagcaccg gcaccctttc tattaacaaa    840

atgccaactg aaatttgaaa agttcaacta aacttatttg acccacaaaa actcgtcaaa    900

agtggcggcg aaagctggca aatgatgaca tccccttgga accatgatat cctctcggaa    960

tcttcgtccc catttgccac atctacttgc aacgccacat ctgcttacta agcaacccaa   1020

atctgcctcg gctcaaaatg tggggaagtt cacatgcatt cgctggtgaa tctgatctga   1080

cactacaact acacaccagg tccaacatga gcgacaatac gacaatcaaa aagccgatcc   1140

gacccaaacc gatccggacg gaacgcctgc cttacgctgg ggccgcagaa atcatccgag   1200

ccaaccagaa agaccactac tttgagtccg tgcttgaaca gcatctcgtc acgtttctgc   1260

agaaatggaa gggagtacga tttatccacc agtacaagga ggagctggag acggcgtcca   1320

agtttgcata tctcggtttg tgtacgcttg tgggctccaa gactctcgga gaagagtaca   1380

ccaatctcat gtacactatc agagaccgaa cagctctacc gggggtggtg agacggtttg   1440

gctacgtgct ttccaacact ctgtttccat acctgtttgt gcgctacatg ggcaagttgc   1500

gcgccaaact gatgcgcgag tatccccatc tggtggagta cgacgaagat gagcctgtgc   1560

ccagcccgga aacatggaag gagcgggtca tcaagacgtt tgtgaacaag tttgacaagt   1620

tcacggcgct ggaggggttt accgcgatcc acttggcgat tttctacgtc tacggctcgt   1680

actaccagct cagtaagcgg atctggggca tgcgttatgt atttggacac cgactggaca   1740

agaatgagcc tcgaatcggt tacgagatgc tcggtctgct gattttcgcc cggtttgcca   1800

cgtcatttgt gcagacggga agagagtacc tcggagcgct gctggaaaag agcgtggaga   1860

aagaggcagg ggagaaggaa gatgaaaagg aagcggttgt gccgaaaaag aagtcgtcaa   1920

ttccgttcat tgaggataca gaaggggaga cggaagacaa gatcgatctg gaggaccctc   1980

gacagctcaa gttcattcct gaggcgtcca gagcgtgcac tctgtgtctg tcatacatta   2040

gtgcgccggc atgtacgcca tgtggacact ttttctgttg ggactgtatt tccgaatggg   2100

tgagagagaa gcccgagtgt cccttgtgtc ggcagggtgt gagagagcag aacttgttgc   2160

ctatcagata atgacgaggt ctggatggaa ggactagtca gcgagacaca gagcatcagg   2220

gaccagacac gaccaattca atcgacaaca ctgtgctgca tagcagtgca cagaggtcct   2280

gggcatgaat atattttagc attggagata tgagtggtag agcgtataca gtattaattg   2340

tggaggtatc tcgtcgcatt gatagagcaa tacagttact gctgaaggga atgataccga   2400

gtatttcggc ccgattcagt tcttgatatc gtcattttgt ctctattgtc tacttttcag   2460

ataacctcaa caaatcttca acaaatctcc cagtaaacag tcagagatca tatccgagat   2520

catatcagat atgtcacgat ccgagtacaa taatggatat taatctgctt gattttgaat   2580

tctgttgcga ttatgatttc tttgatttcg atatgaacac atacggcgac tcccagacct   2640

ttagaagctc cagtttggat tcttagcaat ggttacactc aactatatcc caagtaatac   2700

ttggtaacaa tatgccaagt tagtcattca ttcgttatag gagttagcaa gtgtttgtca   2760

gctaaaaatg gttagtcggt cgattaccac ttagatcttt tcagcgtgga acttgatggt   2820

acgcttgaac cgacacttgg agtagtcggg gctgttgatg acgtagatga cgtttcgctc   2880

agggtgagga gtgcaatagt agtactcctt ggggccgtct ctcagctcaa aggttccatc   2940

ggcggcaatg tcaaagaccg agccctggag cttgtagccg tagtcgccgg tccagaacaa   3000

agcctgcagc tccagatagg cgatgggcat gtcgttaaca gagaaggtgt tgccctcgcc   3060

ctcggtgatg gtgatgggtt cgccgtcggt ggaggcggtg atcaggtcat cttggtaggt   3120

gacgggcaga gattcgaccg attgggcgtc tgatctggta taggtcagct tgtacttgtc   3180

tccgacagcc gccagagcgg tggtagcgac ggtgatgagg gagatgagtt tcatattggc   3240

ggcaagttta gcaaaagatg gcagtgggat tgagggacaa gagtgtttat atagatatag   3300

atacaacaca acgagtctga atgagacaac cgagacaacc actcccgaag cctcactaat   3360

agttactaac ggcatatttc aggtacc                                       3387


<210>  115
<211>  1134
<212>  DNA
<213>  Yarrowia lipolytica (GenBank Accession No. AB036770, nucleotides 1038-2171)


<220>
<221>  CDS
<222>  (1)..(1134)
<223>  Pex10

<400>  115
atg tgg gga agt tca cat gca ttc gct ggt gaa tct gat ctg aca cta       48
Met Trp Gly Ser Ser His Ala Phe Ala Gly Glu Ser Asp Leu Thr Leu         
1               5                   10                  15              

caa cta cac acc agg tcc aac atg agc gac aat acg aca atc aaa aag       96
Gln Leu His Thr Arg Ser Asn Met Ser Asp Asn Thr Thr Ile Lys Lys         
            20                  25                  30                  

ccg atc cga ccc aaa ccg atc cgg acg gaa cgc ctg cct tac gct ggg      144
Pro Ile Arg Pro Lys Pro Ile Arg Thr Glu Arg Leu Pro Tyr Ala Gly         
        35                  40                  45                      

gcc gca gaa atc atc cga gcc aac cag aaa gac cac tac ttt gag tcc      192
Ala Ala Glu Ile Ile Arg Ala Asn Gln Lys Asp His Tyr Phe Glu Ser         
    50                  55                  60                          

gtg ctt gaa cag cat ctc gtc acg ttt ctg cag aaa tgg aag gga gta      240
Val Leu Glu Gln His Leu Val Thr Phe Leu Gln Lys Trp Lys Gly Val         
65                  70                  75                  80          

cga ttt atc cac cag tac aag gag gag ctg gag acg gcg tcc aag ttt      288
Arg Phe Ile His Gln Tyr Lys Glu Glu Leu Glu Thr Ala Ser Lys Phe         
                85                  90                  95              

gca tat ctc ggt ttg tgt acg ctt gtg ggc tcc aag act ctc gga gaa      336
Ala Tyr Leu Gly Leu Cys Thr Leu Val Gly Ser Lys Thr Leu Gly Glu         
            100                 105                 110                 

gag tac acc aat ctc atg tac act atc aga gac cga aca gct cta ccg      384
Glu Tyr Thr Asn Leu Met Tyr Thr Ile Arg Asp Arg Thr Ala Leu Pro         
        115                 120                 125                     

ggg gtg gtg aga cgg ttt ggc tac gtg ctt tcc aac act ctg ttt cca      432
Gly Val Val Arg Arg Phe Gly Tyr Val Leu Ser Asn Thr Leu Phe Pro         
    130                 135                 140                         

tac ctg ttt gtg cgc tac atg ggc aag ttg cgc gcc aaa ctg atg cgc      480
Tyr Leu Phe Val Arg Tyr Met Gly Lys Leu Arg Ala Lys Leu Met Arg         
145                 150                 155                 160         

gag tat ccc cat ctg gtg gag tac gac gaa gat gag cct gtg ccc agc      528
Glu Tyr Pro His Leu Val Glu Tyr Asp Glu Asp Glu Pro Val Pro Ser         
                165                 170                 175             

ccg gaa aca tgg aag gag cgg gtc atc aag acg ttt gtg aac aag ttt      576
Pro Glu Thr Trp Lys Glu Arg Val Ile Lys Thr Phe Val Asn Lys Phe         
            180                 185                 190                 

gac aag ttc acg gcg ctg gag ggg ttt acc gcg atc cac ttg gcg att      624
Asp Lys Phe Thr Ala Leu Glu Gly Phe Thr Ala Ile His Leu Ala Ile         
        195                 200                 205                     

ttc tac gtc tac ggc tcg tac tac cag ctc agt aag cgg atc tgg ggc      672
Phe Tyr Val Tyr Gly Ser Tyr Tyr Gln Leu Ser Lys Arg Ile Trp Gly         
    210                 215                 220                         

atg cgt tat gta ttt gga cac cga ctg gac aag aat gag cct cga atc      720
Met Arg Tyr Val Phe Gly His Arg Leu Asp Lys Asn Glu Pro Arg Ile         
225                 230                 235                 240         

ggt tac gag atg ctc ggt ctg ctg att ttc gcc cgg ttt gcc acg tca      768
Gly Tyr Glu Met Leu Gly Leu Leu Ile Phe Ala Arg Phe Ala Thr Ser         
                245                 250                 255             

ttt gtg cag acg gga aga gag tac ctc gga gcg ctg ctg gaa aag agc      816
Phe Val Gln Thr Gly Arg Glu Tyr Leu Gly Ala Leu Leu Glu Lys Ser         
            260                 265                 270                 

gtg gag aaa gag gca ggg gag aag gaa gat gaa aag gaa gcg gtt gtg      864
Val Glu Lys Glu Ala Gly Glu Lys Glu Asp Glu Lys Glu Ala Val Val         
        275                 280                 285                     

ccg aaa aag aag tcg tca att ccg ttc att gag gat aca gaa ggg gag      912
Pro Lys Lys Lys Ser Ser Ile Pro Phe Ile Glu Asp Thr Glu Gly Glu         
    290                 295                 300                         

acg gaa gac aag atc gat ctg gag gac cct cga cag ctc aag ttc att      960
Thr Glu Asp Lys Ile Asp Leu Glu Asp Pro Arg Gln Leu Lys Phe Ile         
305                 310                 315                 320         

cct gag gcg tcc aga gcg tgc act ctg tgt ctg tca tac att agt gcg     1008
Pro Glu Ala Ser Arg Ala Cys Thr Leu Cys Leu Ser Tyr Ile Ser Ala         
                325                 330                 335             

ccg gca tgt acg cca tgt gga cac ttt ttc tgt tgg gac tgt att tcc     1056
Pro Ala Cys Thr Pro Cys Gly His Phe Phe Cys Trp Asp Cys Ile Ser         
            340                 345                 350                 

gaa tgg gtg aga gag aag ccc gag tgt ccc ttg tgt cgg cag ggt gtg     1104
Glu Trp Val Arg Glu Lys Pro Glu Cys Pro Leu Cys Arg Gln Gly Val         
        355                 360                 365                     

aga gag cag aac ttg ttg cct atc aga taa                             1134
Arg Glu Gln Asn Leu Leu Pro Ile Arg                                     
    370                 375                                             


<210>  116
<211>  377
<212>  PRT
<213>  Yarrowia lipolytica (GenBank Accession No. AB036770, nucleotides 1038-2171)

<400>  116

Met Trp Gly Ser Ser His Ala Phe Ala Gly Glu Ser Asp Leu Thr Leu 
1               5                   10                  15      


Gln Leu His Thr Arg Ser Asn Met Ser Asp Asn Thr Thr Ile Lys Lys 
            20                  25                  30          


Pro Ile Arg Pro Lys Pro Ile Arg Thr Glu Arg Leu Pro Tyr Ala Gly 
        35                  40                  45              


Ala Ala Glu Ile Ile Arg Ala Asn Gln Lys Asp His Tyr Phe Glu Ser 
    50                  55                  60                  


Val Leu Glu Gln His Leu Val Thr Phe Leu Gln Lys Trp Lys Gly Val 
65                  70                  75                  80  


Arg Phe Ile His Gln Tyr Lys Glu Glu Leu Glu Thr Ala Ser Lys Phe 
                85                  90                  95      


Ala Tyr Leu Gly Leu Cys Thr Leu Val Gly Ser Lys Thr Leu Gly Glu 
            100                 105                 110         


Glu Tyr Thr Asn Leu Met Tyr Thr Ile Arg Asp Arg Thr Ala Leu Pro 
        115                 120                 125             


Gly Val Val Arg Arg Phe Gly Tyr Val Leu Ser Asn Thr Leu Phe Pro 
    130                 135                 140                 


Tyr Leu Phe Val Arg Tyr Met Gly Lys Leu Arg Ala Lys Leu Met Arg 
145                 150                 155                 160 


Glu Tyr Pro His Leu Val Glu Tyr Asp Glu Asp Glu Pro Val Pro Ser 
                165                 170                 175     


Pro Glu Thr Trp Lys Glu Arg Val Ile Lys Thr Phe Val Asn Lys Phe 
            180                 185                 190         


Asp Lys Phe Thr Ala Leu Glu Gly Phe Thr Ala Ile His Leu Ala Ile 
        195                 200                 205             


Phe Tyr Val Tyr Gly Ser Tyr Tyr Gln Leu Ser Lys Arg Ile Trp Gly 
    210                 215                 220                 


Met Arg Tyr Val Phe Gly His Arg Leu Asp Lys Asn Glu Pro Arg Ile 
225                 230                 235                 240 


Gly Tyr Glu Met Leu Gly Leu Leu Ile Phe Ala Arg Phe Ala Thr Ser 
                245                 250                 255     


Phe Val Gln Thr Gly Arg Glu Tyr Leu Gly Ala Leu Leu Glu Lys Ser 
            260                 265                 270         


Val Glu Lys Glu Ala Gly Glu Lys Glu Asp Glu Lys Glu Ala Val Val 
        275                 280                 285             


Pro Lys Lys Lys Ser Ser Ile Pro Phe Ile Glu Asp Thr Glu Gly Glu 
    290                 295                 300                 


Thr Glu Asp Lys Ile Asp Leu Glu Asp Pro Arg Gln Leu Lys Phe Ile 
305                 310                 315                 320 


Pro Glu Ala Ser Arg Ala Cys Thr Leu Cys Leu Ser Tyr Ile Ser Ala 
                325                 330                 335     


Pro Ala Cys Thr Pro Cys Gly His Phe Phe Cys Trp Asp Cys Ile Ser 
            340                 345                 350         


Glu Trp Val Arg Glu Lys Pro Glu Cys Pro Leu Cys Arg Gln Gly Val 
        355                 360                 365             


Arg Glu Gln Asn Leu Leu Pro Ile Arg 
    370                 375         


<210>  117
<211>  1065
<212>  DNA
<213>  Yarrowia lipolytica (GenBank Accession No. AJ012084, which corresponds to nucleotides 1107-2171 of GenBank Accession No. AB036770)


<220>
<221>  CDS
<222>  (1)..(1065)
<223>  YlPEX10

<400>  117
atg agc gac aat acg aca atc aaa aag ccg atc cga ccc aaa ccg atc       48
Met Ser Asp Asn Thr Thr Ile Lys Lys Pro Ile Arg Pro Lys Pro Ile         
1               5                   10                  15              

cgg acg gaa cgc ctg cct tac gct ggg gcc gca gaa atc atc cga gcc       96
Arg Thr Glu Arg Leu Pro Tyr Ala Gly Ala Ala Glu Ile Ile Arg Ala         
            20                  25                  30                  

aac cag aaa gac cac tac ttt gag tcc gtg ctt gaa cag cat ctc gtc      144
Asn Gln Lys Asp His Tyr Phe Glu Ser Val Leu Glu Gln His Leu Val         
        35                  40                  45                      

acg ttt ctg cag aaa tgg aag gga gta cga ttt atc cac cag tac aag      192
Thr Phe Leu Gln Lys Trp Lys Gly Val Arg Phe Ile His Gln Tyr Lys         
    50                  55                  60                          

gag gag ctg gag acg gcg tcc aag ttt gca tat ctc ggt ttg tgt acg      240
Glu Glu Leu Glu Thr Ala Ser Lys Phe Ala Tyr Leu Gly Leu Cys Thr         
65                  70                  75                  80          

ctt gtg ggc tcc aag act ctc gga gaa gag tac acc aat ctc atg tac      288
Leu Val Gly Ser Lys Thr Leu Gly Glu Glu Tyr Thr Asn Leu Met Tyr         
                85                  90                  95              

act atc aga gac cga aca gct cta ccg ggg gtg gtg aga cgg ttt ggc      336
Thr Ile Arg Asp Arg Thr Ala Leu Pro Gly Val Val Arg Arg Phe Gly         
            100                 105                 110                 

tac gtg ctt tcc aac act ctg ttt cca tac ctg ttt gtg cgc tac atg      384
Tyr Val Leu Ser Asn Thr Leu Phe Pro Tyr Leu Phe Val Arg Tyr Met         
        115                 120                 125                     

ggc aag ttg cgc gcc aaa ctg atg cgc gag tat ccc cat ctg gtg gag      432
Gly Lys Leu Arg Ala Lys Leu Met Arg Glu Tyr Pro His Leu Val Glu         
    130                 135                 140                         

tac gac gaa gat gag cct gtg ccc agc ccg gaa aca tgg aag gag cgg      480
Tyr Asp Glu Asp Glu Pro Val Pro Ser Pro Glu Thr Trp Lys Glu Arg         
145                 150                 155                 160         

gtc atc aag acg ttt gtg aac aag ttt gac aag ttc acg gcg ctg gag      528
Val Ile Lys Thr Phe Val Asn Lys Phe Asp Lys Phe Thr Ala Leu Glu         
                165                 170                 175             

ggg ttt acc gcg atc cac ttg gcg att ttc tac gtc tac ggc tcg tac      576
Gly Phe Thr Ala Ile His Leu Ala Ile Phe Tyr Val Tyr Gly Ser Tyr         
            180                 185                 190                 

tac cag ctc agt aag cgg atc tgg ggc atg cgt tat gta ttt gga cac      624
Tyr Gln Leu Ser Lys Arg Ile Trp Gly Met Arg Tyr Val Phe Gly His         
        195                 200                 205                     

cga ctg gac aag aat gag cct cga atc ggt tac gag atg ctc ggt ctg      672
Arg Leu Asp Lys Asn Glu Pro Arg Ile Gly Tyr Glu Met Leu Gly Leu         
    210                 215                 220                         

ctg att ttc gcc cgg ttt gcc acg tca ttt gtg cag acg gga aga gag      720
Leu Ile Phe Ala Arg Phe Ala Thr Ser Phe Val Gln Thr Gly Arg Glu         
225                 230                 235                 240         

tac ctc gga gcg ctg ctg gaa aag agc gtg gag aaa gag gca ggg gag      768
Tyr Leu Gly Ala Leu Leu Glu Lys Ser Val Glu Lys Glu Ala Gly Glu         
                245                 250                 255             

aag gaa gat gaa aag gaa gcg gtt gtg ccg aaa aag aag tcg tca att      816
Lys Glu Asp Glu Lys Glu Ala Val Val Pro Lys Lys Lys Ser Ser Ile         
            260                 265                 270                 

ccg ttc att gag gat aca gaa ggg gag acg gaa gac aag atc gat ctg      864
Pro Phe Ile Glu Asp Thr Glu Gly Glu Thr Glu Asp Lys Ile Asp Leu         
        275                 280                 285                     

gag gac cct cga cag ctc aag ttc att cct gag gcg tcc aga gcg tgc      912
Glu Asp Pro Arg Gln Leu Lys Phe Ile Pro Glu Ala Ser Arg Ala Cys         
    290                 295                 300                         

act ctg tgt ctg tca tac att agt gcg ccg gca tgt acg cca tgt gga      960
Thr Leu Cys Leu Ser Tyr Ile Ser Ala Pro Ala Cys Thr Pro Cys Gly         
305                 310                 315                 320         

cac ttt ttc tgt tgg gac tgt att tcc gaa tgg gtg aga gag aag ccc     1008
His Phe Phe Cys Trp Asp Cys Ile Ser Glu Trp Val Arg Glu Lys Pro         
                325                 330                 335             

gag tgt ccc ttg tgt cgg cag ggt gtg aga gag cag aac ttg ttg cct     1056
Glu Cys Pro Leu Cys Arg Gln Gly Val Arg Glu Gln Asn Leu Leu Pro         
            340                 345                 350                 

atc aga taa                                                         1065
Ile Arg                                                                 
                                                                        


<210>  118
<211>  354
<212>  PRT
<213>  Yarrowia lipolytica (GenBank Accession No. AJ012084, which corresponds to nucleotides 1107-2171 of GenBank Accession No. AB036770)

<400>  118

Met Ser Asp Asn Thr Thr Ile Lys Lys Pro Ile Arg Pro Lys Pro Ile 
1               5                   10                  15      


Arg Thr Glu Arg Leu Pro Tyr Ala Gly Ala Ala Glu Ile Ile Arg Ala 
            20                  25                  30          


Asn Gln Lys Asp His Tyr Phe Glu Ser Val Leu Glu Gln His Leu Val 
        35                  40                  45              


Thr Phe Leu Gln Lys Trp Lys Gly Val Arg Phe Ile His Gln Tyr Lys 
    50                  55                  60                  


Glu Glu Leu Glu Thr Ala Ser Lys Phe Ala Tyr Leu Gly Leu Cys Thr 
65                  70                  75                  80  


Leu Val Gly Ser Lys Thr Leu Gly Glu Glu Tyr Thr Asn Leu Met Tyr 
                85                  90                  95      


Thr Ile Arg Asp Arg Thr Ala Leu Pro Gly Val Val Arg Arg Phe Gly 
            100                 105                 110         


Tyr Val Leu Ser Asn Thr Leu Phe Pro Tyr Leu Phe Val Arg Tyr Met 
        115                 120                 125             


Gly Lys Leu Arg Ala Lys Leu Met Arg Glu Tyr Pro His Leu Val Glu 
    130                 135                 140                 


Tyr Asp Glu Asp Glu Pro Val Pro Ser Pro Glu Thr Trp Lys Glu Arg 
145                 150                 155                 160 


Val Ile Lys Thr Phe Val Asn Lys Phe Asp Lys Phe Thr Ala Leu Glu 
                165                 170                 175     


Gly Phe Thr Ala Ile His Leu Ala Ile Phe Tyr Val Tyr Gly Ser Tyr 
            180                 185                 190         


Tyr Gln Leu Ser Lys Arg Ile Trp Gly Met Arg Tyr Val Phe Gly His 
        195                 200                 205             


Arg Leu Asp Lys Asn Glu Pro Arg Ile Gly Tyr Glu Met Leu Gly Leu 
    210                 215                 220                 


Leu Ile Phe Ala Arg Phe Ala Thr Ser Phe Val Gln Thr Gly Arg Glu 
225                 230                 235                 240 


Tyr Leu Gly Ala Leu Leu Glu Lys Ser Val Glu Lys Glu Ala Gly Glu 
                245                 250                 255     


Lys Glu Asp Glu Lys Glu Ala Val Val Pro Lys Lys Lys Ser Ser Ile 
            260                 265                 270         


Pro Phe Ile Glu Asp Thr Glu Gly Glu Thr Glu Asp Lys Ile Asp Leu 
        275                 280                 285             


Glu Asp Pro Arg Gln Leu Lys Phe Ile Pro Glu Ala Ser Arg Ala Cys 
    290                 295                 300                 


Thr Leu Cys Leu Ser Tyr Ile Ser Ala Pro Ala Cys Thr Pro Cys Gly 
305                 310                 315                 320 


His Phe Phe Cys Trp Asp Cys Ile Ser Glu Trp Val Arg Glu Lys Pro 
                325                 330                 335     


Glu Cys Pro Leu Cys Arg Gln Gly Val Arg Glu Gln Asn Leu Leu Pro 
            340                 345                 350         


Ile Arg 
        


<210>  119
<211>  38
<212>  PRT
<213>  Yarrowia lipolytica


<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (5)..(15)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (17)..(17)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (22)..(23)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (25)..(34)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (36)..(37)
<223>  Xaa can be any naturally occurring amino acid

<400>  119

Cys Xaa Xaa Cys Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Cys 
1               5                   10                  15      


Xaa His Xaa Xaa Cys Xaa Xaa Cys Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
            20                  25                  30          


Xaa Xaa Cys Xaa Xaa Cys 
        35              


<210>  120
<211>  345
<212>  PRT
<213>  Yarrowia lipolytica 

<400>  120

Met Trp Gly Ser Ser His Ala Phe Ala Gly Glu Ser Asp Leu Thr Leu 
1               5                   10                  15      


Gln Leu His Thr Arg Ser Asn Met Ser Asp Asn Thr Thr Ile Lys Lys 
            20                  25                  30          


Pro Ile Arg Pro Lys Pro Ile Arg Thr Glu Arg Leu Pro Tyr Ala Gly 
        35                  40                  45              


Ala Ala Glu Ile Ile Arg Ala Asn Gln Lys Asp His Tyr Phe Glu Ser 
    50                  55                  60                  


Val Leu Glu Gln His Leu Val Thr Phe Leu Gln Lys Trp Lys Gly Val 
65                  70                  75                  80  


Arg Phe Ile His Gln Tyr Lys Glu Glu Leu Glu Thr Ala Ser Lys Phe 
                85                  90                  95      


Ala Tyr Leu Gly Leu Cys Thr Leu Val Gly Ser Lys Thr Leu Gly Glu 
            100                 105                 110         


Glu Tyr Thr Asn Leu Met Tyr Thr Ile Arg Asp Arg Thr Ala Leu Pro 
        115                 120                 125             


Gly Val Val Arg Arg Phe Gly Tyr Val Leu Ser Asn Thr Leu Phe Pro 
    130                 135                 140                 


Tyr Leu Phe Val Arg Tyr Met Gly Lys Leu Arg Ala Lys Leu Met Arg 
145                 150                 155                 160 


Glu Tyr Pro His Leu Val Glu Tyr Asp Glu Asp Glu Pro Val Pro Ser 
                165                 170                 175     


Pro Glu Thr Trp Lys Glu Arg Val Ile Lys Thr Phe Val Asn Lys Phe 
            180                 185                 190         


Asp Lys Phe Thr Ala Leu Glu Gly Phe Thr Ala Ile His Leu Ala Ile 
        195                 200                 205             


Phe Tyr Val Tyr Gly Ser Tyr Tyr Gln Leu Ser Lys Arg Ile Trp Gly 
    210                 215                 220                 


Met Arg Tyr Val Phe Gly His Arg Leu Asp Lys Asn Glu Pro Arg Ile 
225                 230                 235                 240 


Gly Tyr Glu Met Leu Gly Leu Leu Ile Phe Ala Arg Phe Ala Thr Ser 
                245                 250                 255     


Phe Val Gln Thr Gly Arg Glu Tyr Leu Gly Ala Leu Leu Glu Lys Ser 
            260                 265                 270         


Val Glu Lys Glu Ala Gly Glu Lys Glu Asp Glu Lys Glu Ala Val Val 
        275                 280                 285             


Pro Lys Lys Lys Ser Ser Ile Pro Phe Ile Glu Asp Thr Glu Gly Glu 
    290                 295                 300                 


Thr Glu Asp Lys Ile Asp Leu Glu Asp Pro Arg Gln Leu Lys Phe Ile 
305                 310                 315                 320 


Pro Glu Ala Ser Arg Ala Cys Thr Leu Cys Leu Ser Tyr Ile Ser Ala 
                325                 330                 335     


Pro Ala Cys Thr Pro Cys Gly His Phe 
            340                 345 


<210>  121
<211>  2987
<212>  DNA
<213>  Yarrowia lipolytica


<220>
<221>  misc_feature
<223>  mutant acetohydroxyacid synthase (AHAS) with W497L mutation

<300>
<302>  HIGH EICOSAPENTAENOIC ACID PRODUCING STRAINS OF YARROWIA 
       LIPOLYTICA
<310>  US 2006-0115881-A1
<311>  2005-11-02
<312>  2006-06-01
<313>  (1)..(2987)

<300>
<302>  HIGH EICOSAPENTAENOIC ACID PRODUCING STRAINS OF YARROWIA 
       LIPOLYTICA
<310>  WO 2006/052870
<311>  2005-11-03
<312>  2006-05-18
<313>  (1)..(2987)

<400>  121
ttccctagtc ccagtgtaca cccgccgata tcgcttaccc tgcagccgga ttaaggttgg     60

caatttttca cgtccttgtc tccgcaatta ctcaccgggt ggtttataag attgcaagcg    120

tcttgatttg tctctgtata ctaacatgca atcgcgactc gcccgacggg ccactaacct    180

ggccagaatc tccagatcca agtattctct tggtctgcga tatgtttcca acacaaaagc    240

ccctgctgcc cagccggcaa ctgctgagtg agtattcctt gccataaacg acccagaacc    300

actgtatagt gtttggaagc actagtcaga agaccagcga aaacaggtgg aaaaaactga    360

gacgaaaagc aacgaccaga aatgtaatgt gtggaaaagc gacacacaca gagcagataa    420

agaggtgaca aataacgaca aatgaaatat cagtatcttc ccacaatcac tacctctcag    480

ctgtctgaag gtgcggctga tatatccatc ccacgtctaa cgtatggagt gtgatagaat    540

atgacgacac aagcatgaga actcgctctc tatccaacca ccgaaacact gtcactacag    600

ccgttcttgt tgctccattc gcttttgtga ttccatgcct tctctggtga ctgacaacat    660

tccttccttt tctccagccc tgttgttatc tgctcatgac ctacggccac tctctatcgc    720

atactaacat agacgatccc agcccgctcc ccacttccag ggcaccgttg gcaagcctcc    780

tatcctcaag aaggctgagg ctgccaacgc tgacatggac gagtccttca tcggaatgtc    840

tggaggagag atcttccacg agatgatgct gcgacacaac gtcgacactg tcttcggtta    900

ccccggtgga gccattctcc ccgtctttga cgccattcac aactctgagt acttcaactt    960

tgtgctccct cgacacgagc agggtgccgg ccacatggcc gagggctacg ctcgagcctc   1020

tggtaagccc ggtgtcgttc tcgtcacctc tggccccggt gccaccaacg tcatcacccc   1080

catgcaggac gctctttccg atggtacccc catggttgtc ttcaccggtc aggtcctgac   1140

ctccgttatc ggcactgacg ccttccagga ggccgatgtt gtcggcatct cccgatcttg   1200

caccaagtgg aacgtcatgg tcaagaacgt tgctgagctc ccccgacgaa tcaacgaggc   1260

ctttgagatt gctacttccg gccgacccgg tcccgttctc gtcgatctgc ccaaggatgt   1320

tactgctgcc atcctgcgag agcccatccc caccaagtcc accattccct cgcattctct   1380

gaccaacctc acctctgccg ccgccaccga gttccagaag caggctatcc agcgagccgc   1440

caacctcatc aaccagtcca agaagcccgt cctttacgtc ggacagggta tccttggctc   1500

cgaggagggt cctaagctgc ttaaggagct ggctgagaag gccgagattc ccgtcaccac   1560

tactctgcag ggtcttggtg cctttgacga gcgagacccc aagtctctgc acatgctcgg   1620

tatgcacggt tccggctacg ccaacatggc catgcagaac gctgactgta tcattgctct   1680

cggcgcccga tttgatgacc gagttaccgg ctccatcccc aagtttgccc ccgaggctcg   1740

agccgctgcc cttgagggtc gaggtggtat tgttcacttt gagatccagg ccaagaacat   1800

caacaaggtt gttcaggcca ccgaagccgt tgagggagac gttaccgagt ctgtccgaca   1860

gctcatcccc ctcatcaaca aggtctctgc cgctgagcga gctccctgga ctgagactat   1920

ccagtcctgg aagcagcagt tccccttcct cttcgaggct gaaggtgagg atggtgttat   1980

caagccccag tccgtcattg ctctgctctc tgacctgaca gagaacaaca aggacaagac   2040

catcatcacc accggtgttg gtcagcatca gatgtggact gcccagcatt tccgatggcg   2100

acaccctcga accatgatca cttctggtgg tcttggaact atgggttacg gcctgcccgc   2160

cgctatcggc gccaaggttg cccgacctga ctgcgacgtc attgacatcg atggtgacgc   2220

ttctttcaac atgactctga ccgagctgtc caccgccgtt cagttcaaca ttggcgtcaa   2280

ggctattgtc ctcaacaacg aggaacaggg tatggtcacc cagctgcagt ctctcttcta   2340

cgagaaccga tactgccaca ctcatcagaa gaaccccgac ttcatgaagc tggccgagtc   2400

catgggcatg aagggtatcc gaatcactca cattgaccag ctggaggccg gtctcaagga   2460

gatgctcgca tacaagggcc ctgtgctcgt tgaggttgtt gtcgacaaga agatccccgt   2520

tcttcccatg gttcccgctg gtaaggcttt gcatgagttc cttgtctacg acgctgacgc   2580

cgaggctgct tctcgacccg atcgactgaa gaatgccccc gcccctcacg tccaccagac   2640

cacctttgag aactaagtgg aaaggaacac aagcaatccg aaccaaaaat aattggggtc   2700

ccgtgcccac agagtctagt gcagacctaa aatgaccaca gtaaattata gctgttatta   2760

aacatgagat tttgaccaac aagagcgtag gaatgttatt agctactact tgtacataca   2820

cagcatttgt tttaaataat gttgcctcca ggggcagtga gatcaggacc cagatccgtg   2880

gccagctctc tgacttcaga ccgcttgtac ttaagcagct cgcaacactg ttgtcgagga   2940

ttgaacttgc catattcgat tttgtggtca tgaatccagc acacctc                 2987


<210>  122
<211>  449
<212>  PRT
<213>  Euglena gracilis


<220>
<221>  MISC_FEATURE
<222>  (34)..(34)
<223>  Xaa = Gly (G) or His (H)

<400>  122

Met Ala Leu Ser Leu Thr Thr Glu Gln Leu Leu Glu Arg Pro Asp Leu 
1               5                   10                  15      


Val Ala Ile Asp Gly Ile Leu Tyr Asp Leu Glu Gly Leu Ala Lys Val 
            20                  25                  30          


His Xaa Gly Gly Asp Leu Ile Leu Ala Ser Gly Ala Ser Asp Ala Ser 
        35                  40                  45              


Pro Leu Phe Tyr Ser Met His Pro Tyr Val Lys Pro Glu Asn Ser Lys 
    50                  55                  60                  


Leu Leu Gln Gln Phe Val Arg Gly Lys His Asp Arg Thr Ser Lys Asp 
65                  70                  75                  80  


Ile Val Tyr Thr Tyr Asp Ser Pro Phe Ala Gln Asp Val Lys Arg Thr 
                85                  90                  95      


Met Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly Phe 
            100                 105                 110         


Trp Leu Arg Thr Val Gly Ile Ile Ala Val Thr Ala Phe Cys Glu Trp 
        115                 120                 125             


His Trp Ala Thr Thr Gly Met Val Leu Trp Gly Leu Leu Thr Gly Phe 
    130                 135                 140                 


Met His Met Gln Ile Gly Leu Ser Ile Gln His Asp Ala Ser His Gly 
145                 150                 155                 160 


Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Leu Phe Ala Tyr Gly Ile 
                165                 170                 175     


Asp Val Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile Met 
            180                 185                 190         


Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala Glu 
        195                 200                 205             


Ser Ala Glu Pro Phe Leu Val Phe His Asn Tyr Pro Ala Ala Asn Thr 
    210                 215                 220                 


Ala Arg Lys Trp Phe His Arg Phe Gln Ala Trp Tyr Met Tyr Leu Val 
225                 230                 235                 240 


Leu Gly Ala Tyr Gly Val Ser Leu Val Tyr Asn Pro Leu Tyr Ile Phe 
                245                 250                 255     


Arg Met Gln His Asn Asp Thr Ile Pro Glu Ser Val Thr Ala Met Arg 
            260                 265                 270         


Glu Asn Gly Phe Leu Arg Arg Tyr Arg Thr Leu Ala Phe Val Met Arg 
        275                 280                 285             


Ala Phe Phe Ile Phe Arg Thr Ala Phe Leu Pro Trp Tyr Leu Thr Gly 
    290                 295                 300                 


Thr Ser Leu Leu Ile Thr Ile Pro Leu Val Pro Thr Ala Thr Gly Ala 
305                 310                 315                 320 


Phe Leu Thr Phe Phe Phe Ile Leu Ser His Asn Phe Asp Gly Ser Glu 
                325                 330                 335     


Arg Ile Pro Asp Lys Asn Cys Lys Val Lys Ser Ser Glu Lys Asp Val 
            340                 345                 350         


Glu Ala Asp Gln Ile Asp Trp Tyr Arg Ala Gln Val Glu Thr Ser Ser 
        355                 360                 365             


Thr Tyr Gly Gly Pro Ile Ala Met Phe Phe Thr Gly Gly Leu Asn Phe 
    370                 375                 380                 


Gln Ile Glu His His Leu Phe Pro Arg Met Ser Ser Trp His Tyr Pro 
385                 390                 395                 400 


Phe Val Gln Gln Ala Val Arg Glu Cys Cys Glu Arg His Gly Val Arg 
                405                 410                 415     


Tyr Val Phe Tyr Pro Thr Ile Val Gly Asn Ile Ile Ser Thr Leu Lys 
            420                 425                 430         


Tyr Met His Lys Val Gly Val Val His Cys Val Lys Asp Ala Gln Asp 
        435                 440                 445             


Ser 
    


<210>  123
<211>  34
<212>  DNA
<213>  Escherichia coli

<400>  123
ataacttcgt ataatgtatg ctatacgaag ttat                                 34


<210>  124
<211>  449
<212>  PRT
<213>  Euglena gracilis

<400>  124

Met Ala Leu Ser Leu Thr Thr Glu Gln Leu Leu Glu Arg Pro Asp Leu 
1               5                   10                  15      


Val Ala Ile Asp Gly Ile Leu Tyr Asp Leu Glu Gly Leu Ala Lys Val 
            20                  25                  30          


His Pro Gly Ser Asp Leu Ile Leu Ala Ser Gly Ala Ser Asp Ala Ser 
        35                  40                  45              


Pro Leu Phe Tyr Ser Met His Pro Tyr Val Lys Pro Glu Asn Ser Lys 
    50                  55                  60                  


Leu Leu Gln Gln Phe Val Arg Gly Lys His Asp Arg Thr Ser Lys Asp 
65                  70                  75                  80  


Ile Val Tyr Thr Tyr Asp Ser Pro Phe Ala Gln Asp Val Lys Arg Thr 
                85                  90                  95      


Met Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly Phe 
            100                 105                 110         


Trp Leu Arg Thr Val Gly Ile Ile Ala Val Thr Ala Phe Cys Glu Trp 
        115                 120                 125             


His Trp Ala Thr Thr Gly Met Val Leu Trp Gly Leu Leu Thr Gly Phe 
    130                 135                 140                 


Met His Met Gln Ile Gly Leu Ser Ile Gln His Asp Ala Ser His Gly 
145                 150                 155                 160 


Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Leu Phe Ala Tyr Gly Ile 
                165                 170                 175     


Asp Val Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile Met 
            180                 185                 190         


Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala Glu 
        195                 200                 205             


Ser Ala Glu Pro Phe Leu Val Phe His Asn Tyr Pro Ala Ala Asn Thr 
    210                 215                 220                 


Ala Arg Lys Trp Phe His Arg Phe Gln Ala Trp Tyr Met Tyr Leu Val 
225                 230                 235                 240 


Leu Gly Ala Tyr Gly Val Ser Leu Val Tyr Asn Pro Leu Tyr Ile Phe 
                245                 250                 255     


Arg Met Gln His Asn Asp Thr Ile Pro Glu Ser Val Thr Ala Met Arg 
            260                 265                 270         


Glu Asn Gly Phe Leu Arg Arg Tyr Arg Thr Leu Ala Phe Val Met Arg 
        275                 280                 285             


Ala Phe Phe Ile Phe Arg Thr Ala Phe Leu Pro Trp Tyr Leu Thr Gly 
    290                 295                 300                 


Thr Ser Leu Leu Ile Thr Ile Pro Leu Val Pro Thr Ala Thr Gly Ala 
305                 310                 315                 320 


Phe Leu Thr Phe Phe Phe Ile Leu Ser His Asn Phe Asp Gly Ser Glu 
                325                 330                 335     


Arg Ile Pro Asp Lys Asn Cys Lys Val Lys Ser Ser Glu Lys Asp Val 
            340                 345                 350         


Glu Ala Asp Gln Ile Asp Trp Tyr Arg Ala Gln Val Glu Thr Ser Ser 
        355                 360                 365             


Thr Tyr Gly Gly Pro Ile Ala Met Phe Phe Thr Gly Gly Leu Asn Phe 
    370                 375                 380                 


Gln Ile Glu His His Leu Phe Pro Arg Met Ser Ser Trp His Tyr Pro 
385                 390                 395                 400 


Phe Val Gln Gln Ala Val Arg Glu Cys Cys Glu Arg His Gly Val Arg 
                405                 410                 415     


Tyr Val Phe Tyr Pro Thr Ile Val Gly Asn Ile Ile Ser Thr Leu Lys 
            420                 425                 430         


Tyr Met His Lys Val Gly Val Val His Cys Val Lys Asp Ala Gln Asp 
        435                 440                 445             


Ser 
    


<210>  125
<211>  454
<212>  PRT
<213>  Euglena anabaena UTEX 373

<400>  125

Met Ala Thr Ile Ser Leu Thr Thr Glu Gln Leu Leu Glu His Pro Glu 
1               5                   10                  15      


Leu Val Ala Ile Asp Gly Val Leu Tyr Asp Leu Phe Gly Leu Ala Lys 
            20                  25                  30          


Val His Cys Gly Gly Asn Leu Ile Glu Ala Ala Gly Ala Ser Asp Gly 
        35                  40                  45              


Thr Ala Leu Phe Tyr Ser Met His Pro Gly Val Lys Pro Glu Asn Ser 
    50                  55                  60                  


Lys Leu Leu Gln Gln Phe Ala Arg Gly Lys His Glu Arg Ser Ser Lys 
65                  70                  75                  80  


Asp Pro Val Tyr Thr Phe Asp Ser Pro Phe Ala Gln Asp Val Lys Gln 
                85                  90                  95      


Ser Val Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly 
            100                 105                 110         


Phe Trp Leu Arg Thr Ala Leu Ile Ile Ala Cys Thr Ala Ile Gly Glu 
        115                 120                 125             


Trp Tyr Trp Ile Thr Thr Gly Ala Val Met Trp Gly Ile Phe Thr Gly 
    130                 135                 140                 


Tyr Phe His Ser Gln Ile Gly Leu Ala Ile Gln His Asp Ala Ser His 
145                 150                 155                 160 


Gly Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Phe Phe Ala Tyr Gly 
                165                 170                 175     


Ile Asp Ala Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile 
            180                 185                 190         


Met Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala 
        195                 200                 205             


Ala Ser Ala Glu Pro Phe Ile Leu Phe His Ser Tyr Pro Ala Thr Asn 
    210                 215                 220                 


Ala Ser Arg Lys Trp Tyr His Arg Phe Gln Ala Trp Tyr Met Tyr Ile 
225                 230                 235                 240 


Val Leu Gly Met Tyr Gly Val Ser Met Val Tyr Asn Pro Met Tyr Leu 
                245                 250                 255     


Phe Thr Met Gln His Asn Asp Thr Ile Pro Glu Ala Thr Ser Leu Arg 
            260                 265                 270         


Pro Gly Ser Phe Phe Asn Arg Gln Arg Ala Phe Ala Val Ser Leu Arg 
        275                 280                 285             


Leu Leu Phe Ile Phe Arg Asn Ala Phe Leu Pro Trp Tyr Ile Ala Gly 
    290                 295                 300                 


Ala Ser Pro Leu Leu Thr Ile Leu Leu Val Pro Thr Val Thr Gly Ile 
305                 310                 315                 320 


Phe Leu Thr Phe Val Phe Val Leu Ser His Asn Phe Glu Gly Ala Glu 
                325                 330                 335     


Arg Thr Pro Glu Lys Asn Cys Lys Ala Lys Arg Ala Lys Glu Gly Lys 
            340                 345                 350         


Glu Val Arg Asp Val Glu Glu Asp Arg Val Asp Trp Tyr Arg Ala Gln 
        355                 360                 365             


Ala Glu Thr Ala Ala Thr Tyr Gly Gly Ser Val Gly Met Met Leu Thr 
    370                 375                 380                 


Gly Gly Leu Asn Leu Gln Ile Glu His His Leu Phe Pro Arg Met Ser 
385                 390                 395                 400 


Ser Trp His Tyr Pro Phe Ile Gln Asp Thr Val Arg Glu Cys Cys Lys 
                405                 410                 415     


Arg His Gly Val Arg Tyr Thr Tyr Tyr Pro Thr Ile Leu Glu Asn Ile 
            420                 425                 430         


Met Ser Thr Leu Arg Tyr Met Gln Lys Val Gly Val Ala His Thr Ile 
        435                 440                 445             


Gln Asp Ala Gln Glu Phe 
    450                 


<210>  126
<211>  463
<212>  PRT
<213>  Peridinium sp. CCMP626


<220>
<221>  MISC_FEATURE
<222>  (55)..(55)
<223>  Xaa = Cys (C) or Trp (W)

<400>  126

Met Ala Pro Asp Ala Asp Lys Leu Arg Gln Arg Lys Ala Gln Ser Ile 
1               5                   10                  15      


Gln Asp Thr Ala Asp Ser Gln Ala Thr Glu Leu Lys Ile Gly Thr Leu 
            20                  25                  30          


Lys Gly Leu Gln Gly Thr Glu Ile Val Ile Asp Gly Asp Ile Tyr Asp 
        35                  40                  45              


Ile Lys Asp Phe Asp His Xaa Gly Gly Glu Ser Ile Met Thr Phe Gly 
    50                  55                  60                  


Gly Asn Asp Val Thr Ala Thr Tyr Lys Met Ile His Pro Tyr His Ser 
65                  70                  75                  80  


Lys His His Leu Glu Lys Met Lys Lys Val Gly Arg Val Pro Asp Tyr 
                85                  90                  95      


Thr Ser Glu Tyr Lys Phe Asp Thr Pro Phe Glu Arg Glu Ile Lys Gln 
            100                 105                 110         


Glu Val Phe Lys Ile Val Arg Arg Gly Arg Glu Phe Gly Thr Pro Gly 
        115                 120                 125             


Tyr Phe Phe Arg Ala Phe Cys Tyr Ile Gly Leu Phe Phe Tyr Leu Gln 
    130                 135                 140                 


Tyr Leu Trp Val Thr Thr Pro Thr Thr Phe Ala Leu Ala Ile Phe Tyr 
145                 150                 155                 160 


Gly Val Ser Gln Ala Phe Ile Gly Leu Asn Val Gln His Asp Ala Asn 
                165                 170                 175     


His Gly Ala Ala Ser Lys Lys Pro Trp Ile Asn Asn Leu Leu Gly Leu 
            180                 185                 190         


Gly Ala Asp Phe Ile Gly Gly Ser Lys Trp Leu Trp Met Asn Gln His 
        195                 200                 205             


Trp Thr His His Thr Tyr Thr Asn His His Glu Lys Asp Pro Asp Ala 
    210                 215                 220                 


Leu Gly Ala Glu Pro Met Leu Leu Phe Asn Asp Tyr Pro Leu Gly His 
225                 230                 235                 240 


Pro Lys Arg Thr Leu Ile His His Phe Gln Ala Phe Tyr Tyr Leu Phe 
                245                 250                 255     


Val Leu Ala Gly Tyr Trp Val Ser Ser Val Phe Asn Pro Gln Ile Leu 
            260                 265                 270         


Asp Leu Gln His Arg Gly Ala Gln Ala Val Gly Met Lys Met Glu Asn 
        275                 280                 285             


Asp Tyr Ile Ala Lys Ser Arg Lys Tyr Ala Ile Phe Leu Arg Leu Leu 
    290                 295                 300                 


Tyr Ile Tyr Thr Asn Ile Val Ala Pro Ile Gln Asn Gln Gly Phe Ser 
305                 310                 315                 320 


Leu Thr Val Val Ala His Ile Leu Thr Met Gly Val Ala Ser Ser Leu 
                325                 330                 335     


Thr Leu Ala Thr Leu Phe Ala Leu Ser His Asn Phe Glu Asn Ala Asp 
            340                 345                 350         


Arg Asp Pro Thr Tyr Glu Ala Arg Lys Gly Gly Glu Pro Val Cys Trp 
        355                 360                 365             


Phe Lys Ser Gln Val Glu Thr Ser Ser Thr Tyr Gly Gly Phe Ile Ser 
    370                 375                 380                 


Gly Cys Leu Thr Gly Gly Leu Asn Phe Gln Val Glu His His Leu Phe 
385                 390                 395                 400 


Pro Arg Met Ser Ser Ala Trp Tyr Pro Tyr Ile Ala Pro Thr Val Arg 
                405                 410                 415     


Glu Val Cys Lys Lys His Gly Val Lys Tyr Ala Tyr Tyr Pro Trp Val 
            420                 425                 430         


Trp Gln Asn Leu Ile Ser Thr Val Lys Tyr Leu His Gln Ser Gly Thr 
        435                 440                 445             


Gly Ser Asn Trp Lys Asn Gly Ala Asn Pro Tyr Ser Gly Lys Leu 
    450                 455                 460             


<210>  127
<211>  13066
<212>  DNA
<213>  Artificial Sequence 

<220>
<223>  Plasmid pZP3-Pa777U

<400>  127
tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa     60

atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt    120

cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tcaggtggca    180

cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata    240

tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga    300

gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc    360

ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg    420

cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc    480

ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat    540

cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact    600

tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat    660

tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga    720

tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc    780

ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga    840

tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag    900

cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc    960

gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt   1020

ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct   1080

acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg   1140

cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg   1200

atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca   1260

tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga   1320

tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa   1380

aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga   1440

aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt   1500

taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt   1560

taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat   1620

agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct   1680

tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca   1740

cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag   1800

agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc   1860

gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga   1920

aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca   1980

tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag   2040

ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg   2100

aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct   2160

ggcgcgccac caatcacaat tctgaaaagc acatcttgat ctcctcattg cggggagtcc   2220

aacggtggtc ttattccccc gaatttcccg ctcaatctcg ttccagaccg acccggacac   2280

agtgcttaac gccgttccga aactctaccg cagatatgct ccaacggact gggctgcata   2340

gatgtgatcc tcggcttgga gaaatggata aaagccggcc aaaaaaaaag cggaaaaaag   2400

cggaaaaaaa gagaaaaaaa atcgcaaaat ttgaaaaata gggggaaaag acgcaaaaac   2460

gcaaggaggg gggagtatat gacactgata agcaagctca caacggttcc tcttattttt   2520

ttcctcatct tctgcctagg ttcccaaaat cccagatgct tctctccagt gccaaaagta   2580

agtaccccac aggttttcgg ccgaaaattc cacgtgcagc aacgtcgtgt ggggtgttaa   2640

aatgtggggg gggggaacca ggacaagagg ctcttgtggg agccgaatga gagcacaaag   2700

cgggcgggtg tgataagggc atttttgccc attttccctt ctcctgtctc tccgacggtg   2760

atggcgttgt gcgtcctcta tttcttttta tttctttttg ttttatttct ctgactaccg   2820

atttggtttg atttcctcaa ccccacacaa ataagctcgg gccgaggaat atatatatac   2880

acggacacag tcgccctgtg gacaacacgt cactacctct acgatacaca ccgtacgttg   2940

tgtggaagct tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg   3000

ccaagctcga aattaaccct cactaaaggg aacaaaagct ggagctccac cgcggacaca   3060

atatctggtc aaatttcagt ttcgttacat ttaaattcct tcacttcaag ttcattcttc   3120

atctgcttct gttttacttt gacaggcaaa tgaagacatg gtacgacttg atggaggcca   3180

agaacgccat ttcaccccga gacaccgaag tgcctgaaat cctggctgcc cccattgata   3240

acatcggaaa ctacggtatt ccggaaagtg tatatagaac ctttccccag cttgtgtctg   3300

tggatatgga tggtgtaatc ccctttgagt actcgtcttg gcttctctcc gagcagtatg   3360

aggctctcta atctagcgca tttaatatct caatgtattt atatatttat cttctcatgc   3420

ggccgcttag ttggctttgg tcttggcagc cttggcctcc ttgagggtaa acatcttggc   3480

atccttgtcg accacgccgt acttggcgta cataagacca attcggatga aggtgggaat   3540

gatgggagaa gccgactttc gcaccagttc gggaaaggcc tgagcgaagg cagcagtggc   3600

ctcgttgagc ttgtagtgag gaatgatggg aaacagatgg tggatctgat gtgtaccaat   3660

gttgtgggac aggttgtcga tgagggctcc gtagcttcgg tccacagagg acaagttgcc   3720

cttgacatag gtccactccg aatcggcgta ccagggagtt tcctcgtcgt tgtgatggag   3780

gaaggtagtg acaaccagca tggtggcgaa tccaaagaga ggtgcgaagt aatacagagc   3840

catggtcttg aggccgtaga cgtaggtaag gtaggcgtac agaccagcaa aggccacgag   3900

agagccgagg gaaatgatga cggcagacat tcttcgcagg tagagaggct cccagggatt   3960

gaagtggttg acctttcggg gaggaaatcc agcaacgagg taggcaaacc aagccgaacc   4020

aagggagatg accatgtgtc gggacagggg atgagagtcg gcttctcgct gagggtagaa   4080

gatctcatcc ttgtcgatgt tgccggtgtt cttgtgatgg tgtcgatggc tgatcttcca   4140

cgactcgtag ggagtcagaa tgatggagtg aatgagtgtg ccaacagaga agttgagcag   4200

gtgggatcgc gagaaggcac catgtccaca gtcgtgaccg atggtaaaga atccccagaa   4260

cacgataccc tggagcagaa tgtagccagt gcaaaggacg gcatcgagca gtgcaaactc   4320

ctgcacgata gcaagggctc gagcatagta cagtccgaga gcaagggaac cggcaatgcc   4380

cagagctcgc acggtatagt agagggacca gggaacagag gcttcgaagc agtgggcagg   4440

cagggatcgc ttgatctcgg tgagagtagg gaactcgtag ggagcggcaa cggtagagga   4500

agccatggtt gtgaattagg gtggtgagaa tggttggttg tagggaagaa tcaaaggccg   4560

gtctcgggat ccgtgggtat atatatatat atatatatat acgatccttc gttacctccc   4620

tgttctcaaa actgtggttt ttcgtttttc gttttttgct ttttttgatt tttttagggc   4680

caactaagct tccagatttc gctaatcacc tttgtactaa ttacaagaaa ggaagaagct   4740

gattagagtt gggcttttta tgcaactgtg ctactcctta tctctgatat gaaagtgtag   4800

acccaatcac atcatgtcat ttagagttgg taatactggg aggatagata aggcacgaaa   4860

acgagccata gcagacatgc tgggtgtagc caagcagaag aaagtagatg ggagccaatt   4920

gacgagcgag ggagctacgc caatccgaca tacgacacgc tgagatcgtc ttggccgggg   4980

ggtacctaca gatgtccaag ggtaagtgct tgactgtaat tgtatgtctg aggacaaata   5040

tgtagtcagc cgtataaagt cataccaggc accagtgcca tcatcgaacc actaactctc   5100

tatgatacat gcctccggta ttattgtacc atgcgtcgct ttgttacata cgtatcttgc   5160

ctttttctct cagaaactcc agactttggc tattggtcga gataagcccg gaccatagtg   5220

agtctttcac actctacatt tctcccttgc tccaactatc gattgttgtc tactaactat   5280

cgtacgataa cttcgtatag catacattat acgaagttat cgcgtcgacg agtatctgtc   5340

tgactcgtca ttgccgcctt tggagtacga ctccaactat gagtgtgctt ggatcacttt   5400

gacgatacat tcttcgttgg aggctgtggg tctgacagct gcgttttcgg cgcggttggc   5460

cgacaacaat atcagctgca acgtcattgc tggctttcat catgatcaca tttttgtcgg   5520

caaaggcgac gcccagagag ccattgacgt tctttctaat ttggaccgat agccgtatag   5580

tccagtctat ctataagttc aactaactcg taactattac cataacatat acttcactgc   5640

cccagataag gttccgataa aaagttctgc agactaaatt tatttcagtc tcctcttcac   5700

caccaaaatg ccctcctacg aagctcgagc taacgtccac aagtccgcct ttgccgctcg   5760

agtgctcaag ctcgtggcag ccaagaaaac caacctgtgt gcttctctgg atgttaccac   5820

caccaaggag ctcattgagc ttgccgataa ggtcggacct tatgtgtgca tgatcaaaac   5880

ccatatcgac atcattgacg acttcaccta cgccggcact gtgctccccc tcaaggaact   5940

tgctcttaag cacggtttct tcctgttcga ggacagaaag ttcgcagata ttggcaacac   6000

tgtcaagcac cagtaccggt gtcaccgaat cgccgagtgg tccgatatca ccaacgccca   6060

cggtgtaccc ggaaccggaa tcattgctgg cctgcgagct ggtgccgagg aaactgtctc   6120

tgaacagaag aaggaggacg tctctgacta cgagaactcc cagtacaagg agttcctagt   6180

cccctctccc aacgagaagc tggccagagg tctgctcatg ctggccgagc tgtcttgcaa   6240

gggctctctg gccactggcg agtactccaa gcagaccatt gagcttgccc gatccgaccc   6300

cgagtttgtg gttggcttca ttgcccagaa ccgacctaag ggcgactctg aggactggct   6360

tattctgacc cccggggtgg gtcttgacga caagggagac gctctcggac agcagtaccg   6420

aactgttgag gatgtcatgt ctaccggaac ggatatcata attgtcggcc gaggtctgta   6480

cggccagaac cgagatccta ttgaggaggc caagcgatac cagaaggctg gctgggaggc   6540

ttaccagaag attaactgtt agaggttaga ctatggatat gtaatttaac tgtgtatata   6600

gagagcgtgc aagtatggag cgcttgttca gcttgtatga tggtcagacg acctgtctga   6660

tcgagtatgt atgatactgc acaacctgtg tatccgcatg atctgtccaa tggggcatgt   6720

tgttgtgttt ctcgatacgg agatgctggg tacagtgcta atacgttgaa ctacttatac   6780

ttatatgagg ctcgaagaaa gctgacttgt gtatgactta ttctcaacta catccccagt   6840

cacaatacca ccactgcact accactacac caaaaccatg atcaaaccac ccatggactt   6900

cctggaggca gaagaacttg ttatggaaaa gctcaagaga gagatcataa cttcgtatag   6960

catacattat acgaagttat cctgcaggta aaggaattca tgctgttcat cgtggttaat   7020

gctgctgtgt gctgtgtgtg tgtgttgttt ggcgctcatt gttgcgttat gcagcgtaca   7080

ccacaatatt ggaagcttat tagcctttct attttttcgt ttgcaaggct taacaacatt   7140

gctgtggaga gggatgggga tatggaggcc gctggaggga gtcggagagg cgttttggag   7200

cggcttggcc tggcgcccag ctcgcgaaac gcacctagga ccctttggca cgccgaaatg   7260

tgccactttt cagtctagta acgccttacc tacgtcattc catgcgtgca tgtttgcgcc   7320

ttttttccct tgcccttgat cgccacacag tacagtgcac tgtacagtgg aggttttggg   7380

ggggtcttag atgggagcta aaagcggcct agcggtacac tagtgggatt gtatggagtg   7440

gcatggagcc taggtggagc ctgacaggac gcacgaccgg ctagcccgtg acagacgatg   7500

ggtggctcct gttgtccacc gcgtacaaat gtttgggcca aagtcttgtc agccttgctt   7560

gcgaacctaa ttcccaattt tgtcacttcg cacccccatt gatcgagccc taacccctgc   7620

ccatcaggca atccaattaa gctcgcattg tctgccttgt ttagtttggc tcctgcccgt   7680

ttcggcgtcc acttgcacaa acacaaacaa gcattatata taaggctcgt ctctccctcc   7740

caaccacact cacttttttg cccgtcttcc cttgctaaca caaaagtcaa gaacacaaac   7800

aaccacccca acccccttac acacaagaca tatctacagc aatggccatg gcttcttcca   7860

ctgttgctgc gccgtacgag ttcccgacgc tgacggagat caagcgctcg ctgccagcgc   7920

actgctttga ggcctcggtc ccgtggtcgc tctactacac cgtgcgcgcg ctgggcatcg   7980

ccggctcgct cgcgctcggc ctctactacg cgcgcgcgct cgcgatcgtg caggagtttg   8040

ccctgctgga tgcggtgctc tgcacggggt acattctgct gcagggcatc gtattctggg   8100

ggttcttcac catcggccat gactgcggcc acggcgcgtt ctcgcgttcg cacctgctca   8160

acttcagcgt cggcacgctc attcactcga tcatcctcac gccgtacgag tcatggaaga   8220

tctcgcaccg ccaccaccac aagaacacgg gcaacatcga caaggacgag attttctacc   8280

cgcagcgcga ggccgactcg cacccactgt cccgacacat ggtgatctcg ctcggctcgg   8340

cctggttcgc gtacctcgtt gcgggcttcc ctcctcgcaa ggtgaaccac ttcaaccctt   8400

gggaaccgtt gtacctgcgc cgcatgtctg ccgtcatcat ctcactcggc tcgctcgtgg   8460

cgttcgcggg cttgtatgcg tatctcacct acgtctatgg ccttaagacc atggcgctgt   8520

actacttcgc ccctctcttt gggttcgcca cgatgctcgt ggtcactacc tttttgcacc   8580

acaatgacga ggaaacgcca tggtacgccg actcggagtg gacgtacgtc aagggcaacc   8640

tctcgtccgt ggaccgctcg tacggcgcgc tcatcgacaa cctgagccac aacatcggca   8700

cgcaccagat ccaccacctg tttccgatca tcccgcacta caagctgaac gaggcgacgg   8760

cagcgttcgc gcaggcgttc ccggagctcg tgcgcaagag cgcgtcgccg atcatcccga   8820

cgttcatccg catcgggctc atgtacgcca agtacggcgt cgtggacaag gacgccaaga   8880

tgtttacgct caaggaggcc aaggccgcca agaccaaggc caactaggcg gccgcattga   8940

tgattggaaa cacacacatg ggttatatct aggtgagagt tagttggaca gttatatatt   9000

aaatcagcta tgccaacggt aacttcattc atgtcaacga ggaaccagtg actgcaagta   9060

atatagaatt tgaccacctt gccattctct tgcactcctt tactatatct catttatttc   9120

ttatatacaa atcacttctt cttcccagca tcgagctcgg aaacctcatg agcaataaca   9180

tcgtggatct cgtcaataga gggctttttg gactccttgc tgttggccac cttgtccttg   9240

ctgtttaaac agtgtacgca gatctactat agaggaacat ttaaattgcc ccggagaaga   9300

cggccaggcc gcctagatga caaattcaac aactcacagc tgactttctg ccattgccac   9360

tagggggggg cctttttata tggccaagcc aagctctcca cgtcggttgg gctgcaccca   9420

acaataaatg ggtagggttg caccaacaaa gggatgggat ggggggtaga agatacgagg   9480

ataacggggc tcaatggcac aaataagaac gaatactgcc attaagactc gtgatccagc   9540

gactgacacc attgcatcat ctaagggcct caaaactacc tcggaactgc tgcgctgatc   9600

tggacaccac agaggttccg agcactttag gttgcaccaa atgtcccacc aggtgcaggc   9660

agaaaacgct ggaacagcgt gtacagtttg tcttaacaaa aagtgagggc gctgaggtcg   9720

agcagggtgg tgtgacttgt tatagccttt agagctgcga aagcgcgtat ggatttggct   9780

catcaggcca gattgagggt ctgtggacac atgtcatgtt agtgtacttc aatcgccccc   9840

tggatatagc cccgacaata ggccgtggcc tcattttttt gccttccgca catttccatt   9900

gctcggtacc cacaccttgc ttctcctgca cttgccaacc ttaatactgg tttacattga   9960

ccaacatctt acaagcgggg ggcttgtcta gggtatatat aaacagtggc tctcccaatc  10020

ggttgccagt ctcttttttc ctttctttcc ccacagattc gaaatctaaa ctacacatca  10080

cagaattccg agccgtgagt atccacgaca agatcagtgt cgagacgacg cgttttgtgt  10140

aatgacacaa tccgaaagtc gctagcaaca cacactctct acacaaacta acccagctct  10200

ggtaccatgg cttcttccac tgttgctgcg ccgtacgagt tcccgacgct gacggagatc  10260

aagcgctcgc tgccagcgca ctgctttgag gcctcggtcc cgtggtcgct ctactacacc  10320

gtgcgcgcgc tgggcatcgc cggctcgctc gcgctcggcc tctactacgc gcgcgcgctc  10380

gcgatcgtgc aggagtttgc cctgctggat gcggtgctct gcacggggta cattctgctg  10440

cagggcatcg tattctgggg gttcttcacc atcggccatg actgcggcca cggcgcgttc  10500

tcgcgttcgc acctgctcaa cttcagcgtc ggcacgctca ttcactcgat catcctcacg  10560

ccgtacgagt catggaagat ctcgcaccgc caccaccaca agaacacggg caacatcgac  10620

aaggacgaga ttttctaccc gcagcgcgag gccgactcgc acccactgtc ccgacacatg  10680

gtgatctcgc tcggctcggc ctggttcgcg tacctcgttg cgggcttccc tcctcgcaag  10740

gtgaaccact tcaacccttg ggaaccgttg tacctgcgcc gcatgtctgc cgtcatcatc  10800

tcactcggct cgctcgtggc gttcgcgggc ttgtatgcgt atctcaccta cgtctatggc  10860

cttaagacca tggcgctgta ctacttcgcc cctctctttg ggttcgccac gatgctcgtg  10920

gtcactacct ttttgcacca caatgacgag gaaacgccat ggtacgccga ctcggagtgg  10980

acgtacgtca agggcaacct ctcgtccgtg gaccgctcgt acggcgcgct catcgacaac  11040

ctgagccaca acatcggcac gcaccagatc caccacctgt ttccgatcat cccgcactac  11100

aagctgaacg aggcgacggc agcgttcgcg caggcgttcc cggagctcgt gcgcaagagc  11160

gcgtcgccga tcatcccgac gttcatccgc atcgggctca tgtacgccaa gtacggcgtc  11220

gtggacaagg acgccaagat gtttacgctc aaggaggcca aggccgccaa gaccaaggcc  11280

aactaggcgg ccgcatggag cgtgtgttct gagtcgatgt tttctatgga gttgtgagtg  11340

ttagtagaca tgatgggttt atatatgatg aatgaataga tgtgattttg atttgcacga  11400

tggaattgag aactttgtaa acgtacatgg gaatgtatga atgtgggggt tttgtgactg  11460

gataactgac ggtcagtgga cgccgttgtt caaatatcca agagatgcga gaaactttgg  11520

gtcaagtgaa catgtcctct ctgttcaagt aaaccatcaa ctatgggtag tatatttagt  11580

aaggacaaga gttgagattc tttggagtcc tagaaacgta ttttcgcgtt ccaagatcaa  11640

attagtagag taatacgggc acgggaatcc attcatagtc tcaatcctgc aggtgagtta  11700

attaagatga cgacatttgc gagctggacg aggaatagat ggagcgtgtg ttctgagtcg  11760

atgttttcta tggagttgtg agtgttagta gacatgatgg gtttatatat gatgaatgaa  11820

tagatgtgat tttgatttgc acgatggaat tgagaacttt gtaaacgtac atgggaatgt  11880

atgaatgtgg gggttttgtg actggataac tgacggtcag tggacgccgt tgttcaaata  11940

tccaagagat gcgagaaact ttgggtcaag tgaacatgtc ctctctgttc aagtaaacca  12000

tcaactatgg gtagtatatt tagtaaggac aagagttgag attctttgga gtcctagaaa  12060

cgtattttcg cgttccaaga tcaaattagt agagtaatac gggcacggga atccattcat  12120

agtctcaatt ttcccatagg tgtgctacaa ggtgttgaga tgtggtacag taccaccatg  12180

attcgaggta aagagcccag aagtcattga tgaggtcaag aaatacacag atctacagct  12240

caatacaatg aatatcttct ttcatattct tcaggtgaca ccaagggtgt ctattttccc  12300

cagaaatgcg tgaaaaggcg cgtgtgtagc gtggagtatg ggttcggttg gcgtatcctt  12360

catatatcga cgaaatagta gggcaagaga tgacaaaaag tatctatatg tagacagcgt  12420

agaatatgga tttgattggt ataaattcat ttattgcgtg tctcacaaat actctcgata  12480

agttggggtt aaactggaga tggaacaatg tcgatatctc gacgcatgcg acgtcgggcc  12540

caattcgccc tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga  12600

ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag  12660

ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa  12720

tggcgaatgg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc  12780

agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc  12840

tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg  12900

ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca  12960

cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc  13020

tttaatagtg gactcttgtt ccaaactgga acaacactca acccta                 13066


<210>  128
<211>  9570
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pY117

<400>  128
ggccgccacc gcggcccgag attccggcct cttcggccgc caagcgaccc gggtggacgt     60

ctagaggtac ctagcaatta acagatagtt tgccggtgat aattctctta acctcccaca    120

ctcctttgac ataacgattt atgtaacgaa actgaaattt gaccagatat tgtgtccgcg    180

gtggagctcc agcttttgtt ccctttagtg agggtttaaa cgagcttggc gtaatcatgg    240

tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa cgtacgagcc    300

ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg    360

ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc    420

ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact    480

gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta    540

atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag    600

caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc    660

cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta    720

taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg    780

ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc    840

tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac    900

gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac    960

ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg   1020

aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga   1080

aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt   1140

agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag   1200

cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct   1260

gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg   1320

atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat   1380

gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc   1440

tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg   1500

gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct   1560

ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca   1620

actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg   1680

ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg   1740

tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc   1800

cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag   1860

ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg   1920

ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag   1980

tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat   2040

agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg   2100

atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca   2160

gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca   2220

aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat   2280

tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag   2340

aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc   2400

tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt   2460

gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc   2520

ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta   2580

cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc   2640

tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg   2700

ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt   2760

ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat   2820

tttaacaaaa tattaacgct tacaatttcc attcgccatt caggctgcgc aactgttggg   2880

aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg   2940

caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg   3000

ccagtgaatt gtaatacgac tcactatagg gcgaattggg taccgggccc cccctcgagg   3060

tcgatggtgt cgataagctt gatatcgaat tcatgtcaca caaaccgatc ttcgcctcaa   3120

ggaaacctaa ttctacatcc gagagactgc cgagatccag tctacactga ttaattttcg   3180

ggccaataat ttaaaaaaat cgtgttatat aatattatat gtattatata tatacatcat   3240

gatgatactg acagtcatgt cccattgcta aatagacaga ctccatctgc cgcctccaac   3300

tgatgttctc aatatttaag gggtcatctc gcattgttta ataataaaca gactccatct   3360

accgcctcca aatgatgttc tcaaaatata ttgtatgaac ttatttttat tacttagtat   3420

tattagacaa cttacttgct ttatgaaaaa cacttcctat ttaggaaaca atttataatg   3480

gcagttcgtt catttaacaa tttatgtaga ataaatgtta taaatgcgta tgggaaatct   3540

taaatatgga tagcataaat gatatctgca ttgcctaatt cgaaatcaac agcaacgaaa   3600

aaaatccctt gtacaacata aatagtcatc gagaaatatc aactatcaaa gaacagctat   3660

tcacacgtta ctattgagat tattattgga cgagaatcac acactcaact gtctttctct   3720

cttctagaaa tacaggtaca agtatgtact attctcattg ttcatacttc tagtcatttc   3780

atcccacata ttccttggat ttctctccaa tgaatgacat tctatcttgc aaattcaaca   3840

attataataa gatataccaa agtagcggta tagtggcaat caaaaagctt ctctggtgtg   3900

cttctcgtat ttatttttat tctaatgatc cattaaaggt atatatttat ttcttgttat   3960

ataatccttt tgtttattac atgggctgga tacataaagg tattttgatt taattttttg   4020

cttaaattca atcccccctc gttcagtgtc aactgtaatg gtaggaaatt accatacttt   4080

tgaagaagca aaaaaaatga aagaaaaaaa aaatcgtatt tccaggttag acgttccgca   4140

gaatctagaa tgcggtatgc ggtacattgt tcttcgaacg taaaagttgc gctccctgag   4200

atattgtaca tttttgcttt tacaagtaca agtacatcgt acaactatgt actactgttg   4260

atgcatccac aacagtttgt tttgtttttt tttgtttttt ttttttctaa tgattcatta   4320

ccgctatgta tacctacttg tacttgtagt aagccgggtt attggcgttc aattaatcat   4380

agacttatga atctgcacgg tgtgcgctgc gagttacttt tagcttatgc atgctacttg   4440

ggtgtaatat tgggatctgt tcggaaatca acggatgctc aaccgatttc gacagtaatt   4500

aattaattcc ctagtcccag tgtacacccg ccgatatcgc ttaccctgca gccggattaa   4560

ggttggcaat ttttcacgtc cttgtctccg caattactca ccgggtggtt tataagattg   4620

caagcgtctt gatttgtctc tgtatactaa catgcaatcg cgactcgccc gacgggccac   4680

taacctggcc agaatctcca gatccaagta ttctcttggt ctgcgatatg tttccaacac   4740

aaaagcccct gctgcccagc cggcaactgc tgagtgagta ttccttgcca taaacgaccc   4800

agaaccactg tatagtgttt ggaagcacta gtcagaagac cagcgaaaac aggtggaaaa   4860

aactgagacg aaaagcaacg accagaaatg taatgtgtgg aaaagcgaca cacacagagc   4920

agataaagag gtgacaaata acgacaaatg aaatatcagt atcttcccac aatcactacc   4980

tctcagctgt ctgaaggtgc ggctgatata tccatcccac gtctaacgta tggagtgtga   5040

tagaatatga cgacacaagc atgagaactc gctctctatc caaccaccga aacactgtca   5100

ctacagccgt tcttgttgct ccattcgctt ttgtgattcc atgccttctc tggtgactga   5160

caacattcct tccttttctc cagccctgtt gttatctgct catgacctac ggccactctc   5220

tatcgcatac taacatagac gatcccagcc cgctccccac ttccagggca ccgttggcaa   5280

gcctcctatc ctcaagaagg ctgaggctgc caacgctgac atggacgagt ccttcatcgg   5340

aatgtctgga ggagagatct tccacgagat gatgctgcga cacaacgtcg acactgtctt   5400

cggttacccc ggtggagcca ttctccccgt ctttgacgcc attcacaact ctgagtactt   5460

caactttgtg ctccctcgac acgagcaggg tgccggccac atggccgagg gctacgctcg   5520

agcctctggt aagcccggtg tcgttctcgt cacctctggc cccggtgcca ccaacgtcat   5580

cacccccatg caggacgctc tttccgatgg tacccccatg gttgtcttca ccggtcaggt   5640

cctgacctcc gttatcggca ctgacgcctt ccaggaggcc gatgttgtcg gcatctcccg   5700

atcttgcacc aagtggaacg tcatggtcaa gaacgttgct gagctccccc gacgaatcaa   5760

cgaggccttt gagattgcta cttccggccg acccggtccc gttctcgtcg atctgcccaa   5820

ggatgttact gctgccatcc tgcgagagcc catccccacc aagtccacca ttccctcgca   5880

ttctctgacc aacctcacct ctgccgccgc caccgagttc cagaagcagg ctatccagcg   5940

agccgccaac ctcatcaacc agtccaagaa gcccgtcctt tacgtcggac agggtatcct   6000

tggctccgag gagggtccta agctgcttaa ggagctggct gagaaggccg agattcccgt   6060

caccactact ctgcagggtc ttggtgcctt tgacgagcga gaccccaagt ctctgcacat   6120

gctcggtatg cacggttccg gctacgccaa catggccatg cagaacgctg actgtatcat   6180

tgctctcggc gcccgatttg atgaccgagt taccggctcc atccccaagt ttgcccccga   6240

ggctcgagcc gctgcccttg agggtcgagg tggtattgtt cactttgaga tccaggccaa   6300

gaacatcaac aaggttgttc aggccaccga agccgttgag ggagacgtta ccgagtctgt   6360

ccgacagctc atccccctca tcaacaaggt ctctgccgct gagcgagctc cctggactga   6420

gactatccag tcctggaagc agcagttccc cttcctcttc gaggctgaag gtgaggatgg   6480

tgttatcaag ccccagtccg tcattgctct gctctctgac ctgacagaga acaacaagga   6540

caagaccatc atcaccaccg gtgttggtca gcatcagatg tggactgccc agcatttccg   6600

atggcgacac cctcgaacca tgatcacttc tggtggtctt ggaactatgg gttacggcct   6660

gcccgccgct atcggcgcca aggttgcccg acctgactgc gacgtcattg acatcgatgg   6720

tgacgcttct ttcaacatga ctctgaccga gctgtccacc gccgttcagt tcaacattgg   6780

cgtcaaggct attgtcctca acaacgagga acagggtatg gtcacccagc tgcagtctct   6840

cttctacgag aaccgatact gccacactca tcagaagaac cccgacttca tgaagctggc   6900

cgagtccatg ggcatgaagg gtatccgaat cactcacatt gaccagctgg aggccggtct   6960

caaggagatg ctcgcataca agggccctgt gctcgttgag gttgttgtcg acaagaagat   7020

ccccgttctt cccatggttc ccgctggtaa ggctttgcat gagttccttg tctacgacgc   7080

tgacgccgag gctgcttctc gacccgatcg actgaagaat gcccccgccc ctcacgtcca   7140

ccagaccacc tttgagaact aagtggaaag gaacacaagc aatccgaacc aaaaataatt   7200

ggggtcccgt gcccacagag tctagtgcag acctaaaatg accacagtaa attatagctg   7260

ttattaaaca tgagattttg accaacaaga gcgtaggaat gttattagct actacttgta   7320

catacacagc atttgtttta aataatgttg cctccagggg cagtgagatc aggacccaga   7380

tccgtggcca gctctctgac ttcagaccgc ttgtacttaa gcagctcgca acactgttgt   7440

cgaggattga acttgccata ttcgattttg tggtcatgaa tccagcacac ctcatttaaa   7500

tgtagctaac ggtagcaggc gaactactgg tacatacctc ccccggaata tgtacaggca   7560

taatgcgtat ctgtgggaca tgtggtcgtt gcgccattat gtaagcagcg tgtactcctc   7620

tgactgtcca tatggtttgc tccatctcac cctcatcgtt ttcattgttc acaggcggcc   7680

acaaaaaaac tgtcttctct ccttctctct tcgccttagt ctactcggac cagttttagt   7740

ttagcttggc gccactggat aaatgagacc tcaggccttg tgatgaggag gtcacttatg   7800

aagcatgtta ggaggtgctt gtatggatag agaagcaccc aaaataataa gaataataat   7860

aaaacagggg gcgttgtcat ttcatatcgt gttttcacca tcaatacacc tccaaacaat   7920

gcccttcatg tggccagccc caatattgtc ctgtagttca actctatgca gctcgtatct   7980

tattgagcaa gtaaaactct gtcagccgat attgcccgac ccgcgacaag ggtcaacaag   8040

gtggtgtaag gccttcgcag aagtcaaaac tgtgccaaac aaacatctag agtctctttg   8100

gtgtttctcg catatatttw atcggctgtc ttacgtattt gcgcctcggt accggactaa   8160

tttcggatca tccccaatac gctttttctt cgcagctgtc aacagtgtcc atgatctatc   8220

cacctaaatg ggtcatatga ggcgtataat ttcgtggtgc tgataataat tcccatatat   8280

ttgacacaaa acttcccccc ctagacatac atctcacaat ctcacttctt gtgcttctgt   8340

cacacatctc ctccagctga cttcaactca cacctctgcc ccagttggtc tacagcggta   8400

taaggtttct ccgcatagag gtgcaccact cctcccgata cttgtttgtg tgacttgtgg   8460

gtcacgacat atatatctac acacattgcg ccaccctttg gttcttccag cacaacaaaa   8520

acacgacacg ctaaccatgg ccaatttact gaccgtacac caaaatttgc ctgcattacc   8580

ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg gacatgttca gggatcgcca   8640

ggcgttttct gagcatacct ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg   8700

gtgcaagttg aataaccgga aatggtttcc cgcagaacct gaagatgttc gcgattatct   8760

tctatatctt caggcgcgcg gtctggcagt aaaaactatc cagcaacatt tgggccagct   8820

aaacatgctt catcgtcggt ccgggctgcc acgaccaagt gacagcaatg ctgtttcact   8880

ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct   8940

agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc atggaaaata gcgatcgctg   9000

ccaggatata cgtaatctgg catttctggg gattgcttat aacaccctgt tacgtatagc   9060

cgaaattgcc aggatcaggg ttaaagatat ctcacgtact gacggtggga gaatgttaat   9120

ccatattggc agaacgaaaa cgctggttag caccgcaggt gtagagaagg cacttagcct   9180

gggggtaact aaactggtcg agcgatggat ttccgtctct ggtgtagctg atgatccgaa   9240

taactacctg ttttgccggg tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca   9300

gctatcaact cgcgccctgg aagggatttt tgaagcaact catcgattga tttacggcgc   9360

taaggatgac tctggtcaga gatacctggc ctggtctgga cacagtgccc gtgtcggagc   9420

cgcgcgagat atggcccgcg ctggagtttc aataccggag atcatgcaag ctggtggctg   9480

gaccaatgta aatattgtca tgaactatat ccgtaacctg gatagtgaaa caggggcaat   9540

ggtgcgcctg ctggaagatg gcgattaagc                                    9570


<210>  129
<211>  15743
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZP2-2988

<400>  129
ggccgcatgt acatacaaga ttatttatag aaatgaatcg cgatcgaaca aagagtacga     60

gtgtacgagt aggggatgat gataaaagtg gaagaagttc cgcatctttg gatttatcaa    120

cgtgtaggac gatacttcct gtaaaaatgc aatgtcttta ccataggttc tgctgtagat    180

gttattaact accattaaca tgtctacttg tacagttgca gaccagttgg agtatagaat    240

ggtacactta ccaaaaagtg ttgatggttg taactacgat atataaaact gttgacggga    300

tctgtatatt cggtaagata tattttgtgg ggttttagtg gtgtttaaac agtgtacgca    360

gtactataga ggaacaattg ccccggagaa gacggccagg ccgcctagat gacaaattca    420

acaactcaca gctgactttc tgccattgcc actagggggg ggccttttta tatggccaag    480

ccaagctctc cacgtcggtt gggctgcacc caacaataaa tgggtagggt tgcaccaaca    540

aagggatggg atggggggta gaagatacga ggataacggg gctcaatggc acaaataaga    600

acgaatactg ccattaagac tcgtgatcca gcgactgaca ccattgcatc atctaagggc    660

ctcaaaacta cctcggaact gctgcgctga tctggacacc acagaggttc cgagcacttt    720

aggttgcacc aaatgtccca ccaggtgcag gcagaaaacg ctggaacagc gtgtacagtt    780

tgtcttaaca aaaagtgagg gcgctgaggt cgagcagggt ggtgtgactt gttatagcct    840

ttagagctgc gaaagcgcgt atggatttgg ctcatcaggc cagattgagg gtctgtggac    900

acatgtcatg ttagtgtact tcaatcgccc cctggatata gccccgacaa taggccgtgg    960

cctcattttt ttgccttccg cacatttcca ttgctcggta cccacacctt gcttctcctg   1020

cacttgccaa ccttaatact ggtttacatt gaccaacatc ttacaagcgg ggggcttgtc   1080

tagggtatat ataaacagtg gctctcccaa tcggttgcca gtctcttttt tcctttcttt   1140

ccccacagat tcgaaatcta aactacacat cacaccatgg aggtcgtgaa cgaaatcgtc   1200

tccattggcc aggaggttct tcccaaggtc gactatgctc agctctggtc tgatgcctcg   1260

cactgcgagg tgctgtacct ctccatcgcc ttcgtcatcc tgaagttcac ccttggtcct   1320

ctcggaccca agggtcagtc tcgaatgaag tttgtgttca ccaactacaa cctgctcatg   1380

tccatctact cgctgggctc cttcctctct atggcctacg ccatgtacac cattggtgtc   1440

atgtccgaca actgcgagaa ggctttcgac aacaatgtct tccgaatcac cactcagctg   1500

ttctacctca gcaagttcct cgagtacatt gactccttct atctgcccct catgggcaag   1560

cctctgacct ggttgcagtt ctttcaccat ctcggagctc ctatggacat gtggctgttc   1620

tacaactacc gaaacgaagc cgtttggatc tttgtgctgc tcaacggctt cattcactgg   1680

atcatgtacg gctactattg gacccgactg atcaagctca agttccctat gcccaagtcc   1740

ctgattactt ctatgcagat cattcagttc aacgttggct tctacatcgt ctggaagtac   1800

cggaacattc cctgctaccg acaagatgga atgagaatgt ttggctggtt tttcaactac   1860

ttctacgttg gtactgtcct gtgtctgttc ctcaacttct acgtgcagac ctacatcgtc   1920

cgaaagcaca agggagccaa aaagattcag tgagcggccg caagtgtgga tggggaagtg   1980

agtgcccggt tctgtgtgca caattggcaa tccaagatgg atggattcaa cacagggata   2040

tagcgagcta cgtggtggtg cgaggatata gcaacggata tttatgtttg acacttgaga   2100

atgtacgata caagcactgt ccaagtacaa tactaaacat actgtacata ctcatactcg   2160

tacccgggca acggtttcac ttgagtgcag tggctagtgc tcttactcgt acagtgtgca   2220

atactgcgta tcatagtctt tgatgtatat cgtattcatt catgttagtt gcgtacgggc   2280

gtcgttgctt gtgtgatttt tgaggaccca tccctttggt atataagtat actctggggt   2340

taaggttgcc cgtgtagtct aggttatagt tttcatgtga aataccgaga gccgagggag   2400

aataaacggg ggtatttgga cttgtttttt tcgcggaaaa gcgtcgaatc aaccctgcgg   2460

gccttgcacc atgtccacga cgtgtttctc gccccaattc gccccttgca cgtcaaaatt   2520

aggcctccat ctagacccct ccataacatg tgactgtggg gaaaagtata agggaaacca   2580

tgcaaccata gacgacgtga aagacgggga ggaaccaatg gaggccaaag aaatggggta   2640

gcaacagtcc aggagacaga caaggagaca aggagagggc gcccgaaaga tcggaaaaac   2700

aaacatgtcc aattggggca gtgacggaaa cgacacggac acttcagtac aatggaccga   2760

ccatctccaa gccagggtta ttccggtatc accttggccg taacctcccg ctggtacctg   2820

atattgtaca cgttcacatt caatatactt tcagctacaa taagagaggc tgtttgtcgg   2880

gcatgtgtgt ccgtcgtatg gggtgatgtc cgagggcgaa attcgctaca agcttaactc   2940

tggcgcttgt ccagtatgaa tagacaagtc aagaccagtg gtgccatgat tgacagggag   3000

gtacaagact tcgatactcg agcattactc ggacttgtgg cgattgaaca gacgggcgat   3060

cgcttctccc ccgtattgcc ggcgcgccag ctgcattaat gaatcggcca acgcgcgggg   3120

agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg   3180

gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca   3240

gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac   3300

cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac   3360

aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg   3420

tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac   3480

ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat   3540

ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag   3600

cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac   3660

ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt   3720

gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt   3780

atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc   3840

aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga   3900

aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac   3960

gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc   4020

cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct   4080

gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca   4140

tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct   4200

ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca   4260

ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc   4320

atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg   4380

cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct   4440

tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa   4500

aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta   4560

tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc   4620

ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg   4680

agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa   4740

gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg   4800

agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc   4860

accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg   4920

gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat   4980

cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata   5040

ggggttccgc gcacatttcc ccgaaaagtg ccacctgatg cggtgtgaaa taccgcacag   5100

atgcgtaagg agaaaatacc gcatcaggaa attgtaagcg ttaatatttt gttaaaattc   5160

gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc   5220

ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag   5280

agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc   5340

gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa   5400

gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg   5460

aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt   5520

gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc   5580

gcgtccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt   5640

cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc   5700

cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac   5760

tatagggcga attgggcccg acgtcgcatg cgctgatgac actttggtct gaaagagatg   5820

cattttgaat cccaaacttg cagtgcccaa gtgacataca tctccgcgtt ttggaaaatg   5880

ttcagaaaca gttgattgtg ttggaatggg gaatggggaa tggaaaaatg actcaagtat   5940

caattccaaa aacttctctg gctggcagta cctactgtcc atactactgc attttctcca   6000

gtcaggccac tctatactcg acgacacagt agtaaaaccc agataatttc gacataaaca   6060

agaaaacaga cccaataata tttatatata gtcagccgtt tgtccagttc agactgtaat   6120

agccgaaaaa aaatccaaag tttctattct aggaaaatat attccaatat ttttaattct   6180

taatctcatt tattttattc tagcgaaata catttcagct acttgagaca tgtgataccc   6240

acaaatcgga ttcggactcg gttgttcaga agagcatatg gcattcgtgc tcgcttgttc   6300

acgtattctt cctgttccat ctcttggccg acaatcacac aaaaatgggg tttttttttt   6360

aattctaatg attcattaca gcaaaattga gatatagcag accacgtatt ccataatcac   6420

caaggaagtt cttgggcgtc ttaattaact cacctgcagg attgagacta tgaatggatt   6480

cccgtgcccg tattactcta ctaatttgat cttggaacgc gaaaatacgt ttctaggact   6540

ccaaagaatc tcaactcttg tccttactaa atatactacc catagttgat ggtttacttg   6600

aacagagagg acatgttcac ttgacccaaa gtttctcgca tctcttggat atttgaacaa   6660

cggcgtccac tgaccgtcag ttatccagtc acaaaacccc cacattcata cattcccatg   6720

tacgtttaca aagttctcaa ttccatcgtg caaatcaaaa tcacatctat tcattcatca   6780

tatataaacc catcatgtct actaacactc acaactccat agaaaacatc gactcagaac   6840

acacgctcca tgcggccgct tactgagcct tggcaccggg ctgcttctcg gccattcgag   6900

cgaactggga caggtatcgg agcaggatga cgagaccttc atggggcaga gggtttcggt   6960

aggggaggtt gtgcttctgg cacagctgtt ccacctggta ggaaacggca gtgaggttgt   7020

gtcgaggcag ggtgggccag agatggtgct cgatctggta gttcaggcct ccaaagaacc   7080

agtcagtaat gatgcctcgt cgaatgttca tggtctcatg gatctgaccc acagagaagc   7140

catgtccgtc ccagacggaa tcaccgatct tctccagagg gtagtggttc atgaagacca   7200

cgatggcaat tccgaagcca ccgacgagct cggaaacaaa gaacaccagc atcgaggtca   7260

ggatggaggg cataaagaag aggtggaaca gggtcttgag agtccagtgc agagcgagtc   7320

caatggcctc tttcttgtac tgagatcggt agaactggtt gtctcggtcc ttgagggatc   7380

gaacggtcag cacagactgg aaacaccaga tgaatcgcag gagaatacag atgaccagga   7440

aatagtactg ttggaactga atgagctttc gggagatggg agaagctcga gtgacatcgt   7500

cctcggacca ggcgagcaga ggcaggttat caatgtcggg atcgtgaccc tgaacgttgg   7560

tagcagaatg atgggcgttg tgtctgtcct tccaccaggt cacggagaag ccctggagtc   7620

cgttgccaaa gaccagaccc aggacgttat tccagtttcg gttcttgaag gtctggtggt   7680

ggcagatgtc atgagacagc catcccattt gctggtagtg cataccgagc acgagagcac   7740

caatgaagta caggtggtac tggaccagca tgaagaaggc aagcacgcca agacccaggg   7800

tggtcaagat cttgtacgag taccagaggg gagaggcgtc aaacatgcca gtggcgatca   7860

gctcttctcg gagctttcgg aaatcctcct gagcttcgtt gacggcagcc tggggaggca   7920

gctcggaagc ctggttgatc ttgggcattc gcttgagctt gtcgaaggct tcctgagagt   7980

gcataaccat gaaggcgtca gtagcatctc gtccctggta gttctcaatg atttcagctc   8040

caccagggtg gaagttcacc caagcggaga cgtcgtacac ctttccgtcg atgacgaggg   8100

gcagagcctg tcgagaagcc ttcaccatgg ttgtgaatta gggtggtgag aatggttggt   8160

tgtagggaag aatcaaaggc cggtctcggg atccgtgggt atatatatat atatatatat   8220

atacgatcct tcgttacctc cctgttctca aaactgtggt ttttcgtttt tcgttttttg   8280

ctttttttga tttttttagg gccaactaag cttccagatt tcgctaatca cctttgtact   8340

aattacaaga aaggaagaag ctgattagag ttgggctttt tatgcaactg tgctactcct   8400

tatctctgat atgaaagtgt agacccaatc acatcatgtc atttagagtt ggtaatactg   8460

ggaggataga taaggcacga aaacgagcca tagcagacat gctgggtgta gccaagcaga   8520

agaaagtaga tgggagccaa ttgacgagcg agggagctac gccaatccga catacgacac   8580

gctgagatcg tcttggccgg ggggtaccta cagatgtcca agggtaagtg cttgactgta   8640

attgtatgtc tgaggacaaa tatgtagtca gccgtataaa gtcataccag gcaccagtgc   8700

catcatcgaa ccactaactc tctatgatac atgcctccgg tattattgta ccatgcgtcg   8760

ctttgttaca tacgtatctt gcctttttct ctcagaaact ccagactttg gctattggtc   8820

gagataagcc cggaccatag tgagtctttc acactctaca tttctccctt gctccaacta   8880

tttaaattcc ttcacttcaa gttcattctt catctgcttc tgttttactt tgacaggcaa   8940

atgaagacat ggtacgactt gatggaggcc aagaacgcca tttcaccccg agacaccgaa   9000

gtgcctgaaa tcctggctgc ccccattgat aacatcggaa actacggtat tccggaaagt   9060

gtatatagaa cctttcccca gcttgtgtct gtggatatgg atggtgtaat cccctttgag   9120

tactcgtctt ggcttctctc cgagcagtat gaggctctct aatctagcgc atttaatatc   9180

tcaatgtatt tatatattta tcttctcatg cggccgctta ctgagccttg gcaccgggct   9240

gcttctcggc cattcgagcg aactgggaca ggtatcggag caggatgacg agaccttcat   9300

ggggcagagg gtttcggtag gggaggttgt gcttctggca cagctgttcc acctggtagg   9360

aaacggcagt gaggttgtgt cgaggcaggg tgggccagag atggtgctcg atctggtagt   9420

tcaggcctcc aaagaaccag tcagtaatga tgcctcgtcg aatgttcatg gtctcatgga   9480

tctgacccac agagaagcca tgtccgtccc agacggaatc accgatcttc tccagagggt   9540

agtggttcat gaagaccacg atggcaattc cgaagccacc gacgagctcg gaaacaaaga   9600

acaccagcat cgaggtcagg atggagggca taaagaagag gtggaacagg gtcttgagag   9660

tccagtgcag agcgagtcca atggcctctt tcttgtactg agatcggtag aactggttgt   9720

ctcggtcctt gagggatcga acggtcagca cagactggaa acaccagatg aatcgcagga   9780

gaatacagat gaccaggaaa tagtactgtt ggaactgaat gagctttcgg gagatgggag   9840

aagctcgagt gacatcgtcc tcggaccagg cgagcagagg caggttatca atgtcgggat   9900

cgtgaccctg aacgttggta gcagaatgat gggcgttgtg tctgtccttc caccaggtca   9960

cggagaagcc ctggagtccg ttgccaaaga ccagacccag gacgttattc cagtttcggt  10020

tcttgaaggt ctggtggtgg cagatgtcat gagacagcca tcccatttgc tggtagtgca  10080

taccgagcac gagagcacca atgaagtaca ggtggtactg gaccagcatg aagaaggcaa  10140

gcacgccaag acccagggtg gtcaagatct tgtacgagta ccagagggga gaggcgtcaa  10200

acatgccagt ggcgatcagc tcttctcgga gctttcggaa atcctcctga gcttcgttga  10260

cggcagcctg gggaggcagc tcggaagcct ggttgatctt gggcattcgc ttgagcttgt  10320

cgaaggcttc ctgagagtgc ataaccatga aggcgtcagt agcatctcgt ccctggtagt  10380

tctcaatgat ttcagctcca ccagggtgga agttcaccca agcggagacg tcgtacacct  10440

ttccgtcgat gacgaggggc agagcctgtc gagaagcctt caccatgggc aggacctgtg  10500

ttagtacatt gtcggggagt catcaattgg ttcgacaggt tgtcgactgt tagtatgagc  10560

tcaattgggc tctggtgggt cgatgacact tgtcatctgt ttctgttggg tcatgtttcc  10620

atcaccttct atggtactca caattcgtcc gattcgcccg aatccgttaa taccgacttt  10680

gatggccatg ttgatgtgtg tttaattcaa gaatgaatat agagaagaga agaagaaaaa  10740

agattcaatt gagccggcga tgcagaccct tatataaatg ttgccttgga cagacggagc  10800

aagcccgccc aaacctacgt tcggtataat atgttaagct ttttaacaca aaggtttggc  10860

ttggggtaac ctgatgtggt gcaaaagacc gggcgttggc gagccattgc gcgggcgaat  10920

ggggccgtga ctcgtctcaa attcgagggc gtgcctcaat tcgtgccccc gtggcttttt  10980

cccgccgttt ccgccccgtt tgcaccactg cagccgcttc tttggttcgg acaccttgct  11040

gcgagctagg tgccttgtgc tacttaaaaa gtggcctccc aacaccaaca tgacatgagt  11100

gcgtgggcca agacacgttg gcggggtcgc agtcggctca atggcccgga aaaaacgctg  11160

ctggagctgg ttcggacgca gtccgccgcg gcgtatggat atccgcaagg ttccatagcg  11220

ccattgccct ccgtcggcgt ctatcccgca acctctaaat agagcgggaa tataacccaa  11280

gcttcttttt tttcctttaa cacgcacacc cccaactatc atgttgctgc tgctgtttga  11340

ctctactctg tggaggggtg ctcccaccca acccaaccta caggtggatc cggcgctgtg  11400

attggctgat aagtctccta tccggactaa ttctgaccaa tgggacatgc gcgcaggacc  11460

caaatgccgc aattacgtaa ccccaacgaa atgcctaccc ctctttggag cccagcggcc  11520

ccaaatcccc ccaagcagcc cggttctacc ggcttccatc tccaagcaca agcagcccgg  11580

aattccttta cctgcaggat aacttcgtat aatgtatgct atacgaagtt atgatctctc  11640

tcttgagctt ttccataaca agttcttctg cctccaggaa gtccatgggt ggtttgatca  11700

tggttttggt gtagtggtag tgcagtggtg gtattgtgac tggggatgta gttgagaata  11760

agtcatacac aagtcagctt tcttcgagcc tcatataagt ataagtagtt caacgtatta  11820

gcactgtacc cagcatctcc gtatcgagaa acacaacaac atgccccatt ggacagatca  11880

tgcggataca caggttgtgc agtatcatac atactcgatc agacaggtcg tctgaccatc  11940

atacaagctg aacaagcgct ccatacttgc acgctctcta tatacacagt taaattacat  12000

atccatagtc taacctctaa cagttaatct tctggtaagc ctcccagcca gccttctggt  12060

atcgcttggc ctcctcaata ggatctcggt tctggccgta cagacctcgg ccgacaatta  12120

tgatatccgt tccggtagac atgacatcct caacagttcg gtactgctgt ccgagagcgt  12180

ctcccttgtc gtcaagaccc accccggggg tcagaataag ccagtcctca gagtcgccct  12240

taggtcggtt ctgggcaatg aagccaacca caaactcggg gtcggatcgg gcaagctcaa  12300

tggtctgctt ggagtactcg ccagtggcca gagagccctt gcaagacagc tcggccagca  12360

tgagcagacc tctggccagc ttctcgttgg gagaggggac taggaactcc ttgtactggg  12420

agttctcgta gtcagagacg tcctccttct tctgttcaga gacagtttcc tcggcaccag  12480

ctcgcaggcc agcaatgatt ccggttccgg gtacaccgtg ggcgttggtg atatcggacc  12540

actcggcgat tcggtgacac cggtactggt gcttgacagt gttgccaata tctgcgaact  12600

ttctgtcctc gaacaggaag aaaccgtgct taagagcaag ttccttgagg gggagcacag  12660

tgccggcgta ggtgaagtcg tcaatgatgt cgatatgggt tttgatcatg cacacataag  12720

gtccgacctt atcggcaagc tcaatgagct ccttggtggt ggtaacatcc agagaagcac  12780

acaggttggt tttcttggct gccacgagct tgagcactcg agcggcaaag gcggacttgt  12840

ggacgttagc tcgagcttcg taggagggca ttttggtggt gaagaggaga ctgaaataaa  12900

tttagtctgc agaacttttt atcggaacct tatctggggc agtgaagtat atgttatggt  12960

aatagttacg agttagttga acttatagat agactggact atacggctat cggtccaaat  13020

tagaaagaac gtcaatggct ctctgggcgt cgcctttgcc gacaaaaatg tgatcatgat  13080

gaaagccagc aatgacgttg cagctgatat tgttgtcggc caaccgcgcc gaaaacgcag  13140

ctgtcagacc cacagcctcc aacgaagaat gtatcgtcaa agtgatccaa gcacactcat  13200

agttggagtc gtactccaaa ggcggcaatg acgagtcaga cagatactcg tcgacgcgat  13260

aacttcgtat aatgtatgct atacgaagtt atcgtacgat agttagtaga caacaatcga  13320

taacgtctcg taccaaccac agattacgac ccattcgcag tcacagttca ctagggtttg  13380

ggttgcatcc gttgagagcg gtttgttttt aaccttctcc atgtgctcac tcaggttttg  13440

ggttcagatc aaatcaaggc gtgaaccact ttgtttgagg acaaatgtga cacaaccaac  13500

cagtgtcagg ggcaagtccg tgacaaaggg gaagatacaa tgcaattact gacagttaca  13560

gactgcctcg atgccctaac cttgccccaa aataagacaa ctgtcctcgt ttaagcgcaa  13620

ccctattcag cgtcacgtca taatagcgtt tggatagcac tagtctatga ggagcgtttt  13680

atgttgcggt gagggcgatt ggtgctcata tgggttcaat tgaggtggcg gaacgagctt  13740

agtcttcaat tgaggtgcga gcgacacaat tgggtgtcac gtggcctaat tgacctcggg  13800

tcgtggagtc cccagttata cagcaaccac gaggtgcatg ggtaggagac gtcaccagac  13860

aatagggttt tttttggact ggagagggtt gggcaaaagc gctcaacggg ctgtttgggg  13920

agctgtgggg gaggaattgg cgatatttgt gaggttaacg gctccgattt gcgtgttttg  13980

tcgctcctgc atctccccat acccatatct tccctcccca cctctttcca cgataatttt  14040

acggatcagc aataaggttc cttctcctag tttccacgtc catatatatc tatgctgcgt  14100

cgtccttttc gtgacatcac caaaacacat acaacaatgg ctgttactga cgtccttaag  14160

cgaaagtccg gtgtcatcgt cggcgacgat gtccgagccg tgagtatcca cgacaagatc  14220

agtgtcgaga cgacgcgttt tgtgtaatga cacaatccga aagtcgctag caacacacac  14280

tctctacaca aactaaccca gctctccatg gcctccacct cggctctgcc caagcagaac  14340

cctgccctcc gacgaaccgt cacttccacc actgtgaccg actcggagtc tgctgccgtc  14400

tctccctccg attctcccag acactcggcc tcctctacat cgctgtcttc catgtccgag  14460

gtggacattg ccaagcccaa gtccgagtac ggtgtcatgc tggataccta cggcaaccag  14520

ttcgaagttc ccgacttcac catcaaggac atctacaacg ctattcccaa gcactgcttc  14580

aagcgatctg ctctcaaggg atacggctac attcttcgag acattgtcct cctgactacc  14640

actttcagca tctggtacaa ctttgtgaca cccgagtaca ttccctccac tcctgctcga  14700

gccggtctgt gggctgtgta caccgttctt cagggactct tcggtactgg actgtgggtc  14760

attgcccacg agtgtggaca tggtgctttc tccgattccc gaatcatcaa cgacattact  14820

ggctgggtgc ttcactcttc cctgcttgtt ccctacttca gctggcaaat ctcccaccgg  14880

aagcatcaca aggccactgg aaacatggag cgagacatgg tcttcgttcc tcgaacccga  14940

gagcagcaag ctactcgact cggcaagatg acccacgaac tcgcccatct taccgaggaa  15000

actcctgctt tcaccctgct catgcttgtg cttcagcaac tggtcggttg gcccaactat  15060

ctcattacca acgttactgg acacaactac catgagcggc agcgagaggg tcgaggcaag  15120

ggaaagcaca acggtcttgg cggtggagtt aaccatttcg atccccgatc tcctctgtac  15180

gagaacagcg acgccaagct catcgtgctc tccgacattg gcattggtct tatggccacc  15240

gctctgtact ttctcgttca gaagttcgga ttctacaaca tggccatctg gtacttcgtt  15300

ccctacttgt gggttaacca ctggctcgtc gccattacct ttctgcagca cacagatcct  15360

actcttcccc actacaccaa cgacgagtgg aactttgtgc gaggtgccgc tgcaaccatc  15420

gaccgagaga tgggcttcat tggacgtcat ctgctccacg gcattatcga gactcacgtc  15480

ctgcatcact acgtctcttc cattcccttc tacaatgcgg acgaagctac cgaggccatc  15540

aaacctatca tgggcaagca ctatcgagct gatgtccagg acggtcctcg aggattcatt  15600

cgagccatgt accgatctgc acgaatgtgc cagtgggttg aaccctccgc tggtgccgag  15660

ggagctggca agggtgtcct gttctttcga aaccgaaaca atgtgggcac tcctcccgct  15720

gtcatcaagc ccgttgccta agc                                          15743


<210>  130
<211>  6303
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZKUE3S

<400>  130
ggccgcaagt gtggatgggg aagtgagtgc ccggttctgt gtgcacaatt ggcaatccaa     60

gatggatgga ttcaacacag ggatatagcg agctacgtgg tggtgcgagg atatagcaac    120

ggatatttat gtttgacact tgagaatgta cgatacaagc actgtccaag tacaatacta    180

aacatactgt acatactcat actcgtaccc gggcaacggt ttcacttgag tgcagtggct    240

agtgctctta ctcgtacagt gtgcaatact gcgtatcata gtctttgatg tatatcgtat    300

tcattcatgt tagttgcgta cgaggaaact gtctctgaac agaagaagga ggacgtctct    360

gactacgaga actcccagta caaggagttc ctagtcccct ctcccaacga gaagctggcc    420

agaggtctgc tcatgctggc cgagctgtct tgcaagggct ctctggccac tggcgagtac    480

tccaagcaga ccattgagct tgcccgatcc gaccccgagt ttgtggttgg cttcattgcc    540

cagaaccgac ctaagggcga ctctgaggac tggcttattc tgacccccgg ggtgggtctt    600

gacgacaagg gagacgctct cggacagcag taccgaactg ttgaggatgt catgtctacc    660

ggaacggata tcataattgt cggccgaggt ctgtacggcc agaaccgaga tcctattgag    720

gaggccaagc gataccagaa ggctggctgg gaggcttacc agaagattaa ctgttagagg    780

ttagactatg gatatgtaat ttaactgtgt atatagagag cgtgcaagta tggagcgctt    840

gttcagcttg tatgatggtc agacgacctg tctgatcgag tatgtatgat actgcacaac    900

ctgtgtatcc gcatgatctg tccaatgggg catgttgttg tgtttctcga tacggagatg    960

ctgggtacag tgctaatacg ttgaactact tatacttata tgaggctcga agaaagctga   1020

cttgtgtatg acttaattaa tcgagcttgg cgtaatcatg gtcatagctg tttcctgtgt   1080

gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag   1140

cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt   1200

tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag   1260

gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg   1320

ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat   1380

caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta   1440

aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa   1500

atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc   1560

cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt   1620

ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca   1680

gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg   1740

accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat   1800

cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta   1860

cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct   1920

gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac   1980

aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa   2040

aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa   2100

actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt   2160

taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca   2220

gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca   2280

tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc   2340

ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa   2400

accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc   2460

agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca   2520

acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat   2580

tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag   2640

cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac   2700

tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt   2760

ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt   2820

gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc   2880

tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat   2940

ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca   3000

gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga   3060

cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg   3120

gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg   3180

ttccgcgcac atttccccga aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg   3240

cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg   3300

ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc   3360

taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa   3420

aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc   3480

ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac   3540

tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt   3600

ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc   3660

ttacaatttc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc   3720

ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt   3780

aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat tgtaatacga   3840

ctcactatag ggcgaattgg gtaccgggcc ccccctcgag gtcgacgagt atctgtctga   3900

ctcgtcattg catgcctttg gagtacgact ccaactatga gtgtgcttgg atcactttga   3960

cgatacattc ttcgttggag gctgtgggtc tgacagctgc gttttcggcg cggttggccg   4020

acaacaatat cagctgcaac gtcattgctg gctttcatca tgatcacatt tttgtcggca   4080

aaggcgacgc ccagagagcc attgacgttc tttctaattt ggaccgatag ccgtatagtc   4140

cagtctatct ataagttcaa ctaactcgta actattacca taacatatac ttcactgccc   4200

cagataaggt tccgataaaa agttctgcag actaaattta tttcagtctc ctcttcacca   4260

ccaaaatgcc ctcctacgaa gctcgagtgc tcaagctcgt ggcagccaag aaaaccaacc   4320

tgtgtgcttc tctggatgtt accaccacca aggagctcat tgagcttgcc gataaggtcg   4380

gaccttatgt gtgcatgatc aaaacccata tcgacatcat tgacgacttc acctacgccg   4440

gcactgtgct ccccctcaag gaacttgctc ttaagcacgg tttcttcctg ttcgaggaca   4500

gaaagttcgc agatattggc aacactgtca agcaccagta ccggtgtcac cgaatcgccg   4560

agtggtccga tatcaccaac gcccacggtg tttaaacccg gaaccggaat cgataagctt   4620

gatatcgaat tcatgctgtt catcgtggtt aatgctgctg tgtgctgtgt gtgtgtgttg   4680

tttggcgctc attgttgcgt tatgcagcgt acaccacaat attggaagct tattagcctt   4740

tctatttttt cgtttgcaag gcttaacaac attgctgtgg agagggatgg ggatatggag   4800

gccgctggag ggagtcggag aggcgttttg gagcggcttg gcctggcgcc cagctcgcga   4860

aacgcaccta ggaccctttg gcacgccgaa atgtgccact tttcagtcta gtaacgcctt   4920

acctacgtca ttccatgcgt gcatgtttgc gccttttttc ccttgccctt gatcgccaca   4980

cagtacagtg cactgtacag tggaggtttt gggggggtct tagatgggag ctaaaagcgg   5040

cctagcggta cactagtggg attgtatgga gtggcatgga gcctaggtgg agcctgacag   5100

gacgcacgac cggctagccc gtgacagacg atgggtggct cctgttgtcc accgcgtaca   5160

aatgtttggg ccaaagtctt gtcagccttg cttgcgaacc taattcccaa ttttgtcact   5220

tcgcaccccc attgatcgag ccctaacccc tgcccatcag gcaatccaat taagctcgca   5280

ttgtctgcct tgtttagttt ggctcctgcc cgtttcggcg tccacttgca caaacacaaa   5340

caagcattat atataaggct cgtctctccc tcccaaccac actcactttt ttgcccgtct   5400

tcccttgcta acacaaaagt caagaacaca aacaaccacc ccaaccccct tacacacaag   5460

acatatctac accatggagt ctggacccat gcctgctggc attcccttcc ctgagtacta   5520

tgacttcttt atggactgga agactcccct ggccatcgct gccacctaca ctgctgccgt   5580

cggtctcttc aaccccaagg ttggcaaggt ctcccgagtg gttgccaagt cggctaacgc   5640

aaagcctgcc gagcgaaccc agtccggagc tgccatgact gccttcgtct ttgtgcacaa   5700

cctcattctg tgtgtctact ctggcatcac cttctactac atgtttcctg ctatggtcaa   5760

gaacttccga acccacacac tgcacgaagc ctactgcgac acggatcagt ccctctggaa   5820

caacgcactt ggctactggg gttacctctt ctacctgtcc aagttctacg aggtcattga   5880

caccatcatc atcatcctga agggacgacg gtcctcgctg cttcagacct accaccatgc   5940

tggagccatg attaccatgt ggtctggcat caactaccaa gccactccca tttggatctt   6000

tgtggtcttc aactccttca ttcacaccat catgtactgt tactatgcct tcacctctat   6060

cggattccat cctcctggca aaaagtacct gacttcgatg cagattactc agtttctggt   6120

cggtatcacc attgccgtgt cctacctctt cgttcctggc tgcatccgaa cacccggtgc   6180

tcagatggct gtctggatca acgtcggcta cctgtttccc ttgacctatc tgttcgtgga   6240

ctttgccaag cgaacctact ccaagcgatc tgccattgcc gctcagaaaa aggctcagta   6300

agc                                                                 6303


<210>  131
<211>  15812
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZKL2-5U89GC

<400>  131
gtacgttatc atttgaacag tgaaaggcta cagtaacaga agcagttgta aacttcattc     60

cgttgattct gtactacagt accccactac gccgcttccg ctgacactgt tcaacccaaa    120

aactacatct gcgtgcgctg tgtaaggcta tcatcagata catactgtag attctgtaga    180

tgcgaacctg cttgtatcat atacatcccc ctccccctga cctgcacaag caagcaatgt    240

gacattgata ttgctgctta tctagtgccg aggatgtgaa agccgagact caaacatttc    300

ttttactctc ttgttcctga ccagacctgg cggagattac gccagtatga ttcttgcagg    360

tctgagacaa gcctggaaca gccaacattt atttttcgaa gcgagaaaca tgccacaccc    420

cggcacgttc agagatgcat atgatttgtt tttcgagtaa cagtaccccc cccccccccc    480

ccaatgaaac cagtattact cacaccatcc tcattcaaag cgttacactg attacgcgcc    540

catcaacgac agcatgaggg gactgctgat ctgatctaat caaatgacta caaaaatcgc    600

aataatgaag agcaaacgac aaaaaagaaa caggttaacc aatcccgctt caatgtctca    660

ccacaatcca gcactgtttc tcattacctc ctccctctaa tttcagagtt gcatcagggt    720

ccttgatggc gcgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc    780

gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc    840

ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata    900

acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg    960

cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct   1020

caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa   1080

gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc   1140

tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt   1200

aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg   1260

ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg   1320

cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct   1380

tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc   1440

tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg   1500

ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc   1560

aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt   1620

aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa   1680

aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat   1740

gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct   1800

gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg   1860

caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag   1920

ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta   1980

attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg   2040

ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg   2100

gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct   2160

ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta   2220

tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg   2280

gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc   2340

cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg   2400

gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga   2460

tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg   2520

ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat   2580

gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc   2640

tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca   2700

catttccccg aaaagtgcca cctgatgcgg tgtgaaatac cgcacagatg cgtaaggaga   2760

aaataccgca tcaggaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt   2820

gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa   2880

aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa   2940

agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac   3000

gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga   3060

accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa   3120

aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc   3180

tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tccattcgcc   3240

attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca   3300

gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca   3360

gtcacgacgt tgtaaaacga cggccagtga attgtaatac gactcactat agggcgaatt   3420

gggcccgacg tcgcatgctg gtttcgattt gtcttagagg aacgcatata cagtaatcat   3480

agagaataaa cgatattcat ttattaaagt agatagttga ggtagaagtt gtaaagagtg   3540

ataaatagct tagataccac agacaccctc ggtgacgaag tactgcagat ggtttccaat   3600

cacattgacc tgctggagca gagtgttacc ggcagagcac tgtttattgc tctggccctg   3660

gcacatgaca acgttggaga gaggagggtg gatcaggggc cagtcaataa agacctcacc   3720

agagcagtgc tggtaaccgt cccagaaggg cacttgaggg acgatatctc ctcggtgggt   3780

gattcggtag agctttcggt ctttggacac cttggagaca tcggggttct cctggccaaa   3840

gaagagttta tcgacccagt tagcaaagcc agcgttaccg acaatgggct gaccaagagt   3900

aacaacgagg ggatcgtggc cgttaacctt gaggttgatt ccgaacagaa gggctgcagc   3960

tcctccgaga gagtgaccgg tgacagcaat ctggtagtcg ggatactgct caatcacaga   4020

gtcgagcttg gggccgatct gattgtaggt gttgttgtag gactggatga agccattgtg   4080

gacaagacag tcatcacaag tagcagtaga agagatgtta gcagcaagat caaagttaat   4140

taactcacct gcaggattga gactatgaat ggattcccgt gcccgtatta ctctactaat   4200

ttgatcttgg aacgcgaaaa tacgtttcta ggactccaaa gaatctcaac tcttgtcctt   4260

actaaatata ctacccatag ttgatggttt acttgaacag agaggacatg ttcacttgac   4320

ccaaagtttc tcgcatctct tggatatttg aacaacggcg tccactgacc gtcagttatc   4380

cagtcacaaa acccccacat tcatacattc ccatgtacgt ttacaaagtt ctcaattcca   4440

tcgtgcaaat caaaatcaca tctattcatt catcatatat aaacccatca tgtctactaa   4500

cactcacaac tccatagaaa acatcgactc agaacacacg ctccatgcgg ccgcttagga   4560

atcctgagcg tccttgacac agtgaaccac accgactttg tgcatgtact tgagggtgga   4620

aatgatgttg cccacaatgg tagggtagaa gacgtaccga actccgtgtc gttcgcaaca   4680

ctctcggaca gcttgctgca cgaagggata gtgccaagac gacattcgag gaaagaggtg   4740

atgctcgatc tggaagttga gaccgccagt aaagaacatg gcaatgggtc caccgtaggt   4800

ggaagaggtc tccacctgag ctctgtacca gtcgatctga tcggcttcaa cgtccttctc   4860

ggagctcttg accttgcagt tcttgtcggg gattcgctcc gagccatcga agttgtgaga   4920

caagatgaaa aagaaggtga ggaaggcacc ggtagcagtg ggcaccagag gaatggtgat   4980

gagcagggag gttccagtga gataccaggg caagaaggcg gttcgaaaga tgaagaaagc   5040

tcgcataacg aatgcaaggg ttcggtaccg tcgcagaaag ccgttctctc gcatggctgt   5100

gacagactcg ggaatggtgt cgttgtgctg cattcggaag atgtagagag ggttgtacac   5160

cagcgaaacg ccgtaggctc caagcacgag gtacatgtac caggcctgga atcggtgaaa   5220

ccactttcga gcagtgttgg cagcagggta gttgtggaac acaaggaatg gttctgcgga   5280

ctcggcatcc aggtcgagac catgctgatt ggtgtaggtg tgatgtcgca tgatgtgaga   5340

ctgcagccag atccatctgg acgatccaat gacgtcgatg ccgtaggcaa agagagcgtt   5400

gacccagggc tttttgctga tggcaccatg agaggcatcg tgctgaatgg acaggccgat   5460

ctgcatgtgc atgaatccag tcaagagacc ccacagcacc attccggtag tagcccagtg   5520

ccactcgcaa aaggcggtga cagcaatgat gccaacggtt cgcagccaga atccaggtgt   5580

ggcataccag ttccgacctt tcatgacctc tcgcatagtt cgcttgacgt cctgtgcaaa   5640

gggagagtcg taggtgtaga caatgtcctt ggaggttcgg tcgtgcttgc ctcgcacgaa   5700

ctgttgaagc agcttcgagt tctcgggctt gacgtaaggg tgcatggagt agaacagagg   5760

agaagcatcg gaggcaccag aagcgaggat caagtcgcct ccgggatgga ccttggcaag   5820

accttccaga tcgtagagaa tgccgtcgat ggcaaccagg tcgggtcgct cgagcagctg   5880

ctcggtagta agggagagag ccatggttgt gaattagggt ggtgagaatg gttggttgta   5940

gggaagaatc aaaggccggt ctcgggatcc gtgggtatat atatatatat atatatatac   6000

gatccttcgt tacctccctg ttctcaaaac tgtggttttt cgtttttcgt tttttgcttt   6060

ttttgatttt tttagggcca actaagcttc cagatttcgc taatcacctt tgtactaatt   6120

acaagaaagg aagaagctga ttagagttgg gctttttatg caactgtgct actccttatc   6180

tctgatatga aagtgtagac ccaatcacat catgtcattt agagttggta atactgggag   6240

gatagataag gcacgaaaac gagccatagc agacatgctg ggtgtagcca agcagaagaa   6300

agtagatggg agccaattga cgagcgaggg agctacgcca atccgacata cgacacgctg   6360

agatcgtctt ggccgggggg tacctacaga tgtccaaggg taagtgcttg actgtaattg   6420

tatgtctgag gacaaatatg tagtcagccg tataaagtca taccaggcac cagtgccatc   6480

atcgaaccac taactctcta tgatacatgc ctccggtatt attgtaccat gcgtcgcttt   6540

gttacatacg tatcttgcct ttttctctca gaaactccag aattctctct cttgagcttt   6600

tccataacaa gttcttctgc ctccaggaag tccatgggtg gtttgatcat ggttttggtg   6660

tagtggtagt gcagtggtgg tattgtgact ggggatgtag ttgagaataa gtcatacaca   6720

agtcagcttt cttcgagcct catataagta taagtagttc aacgtattag cactgtaccc   6780

agcatctccg tatcgagaaa cacaacaaca tgccccattg gacagatcat gcggatacac   6840

aggttgtgca gtatcataca tactcgatca gacaggtcgt ctgaccatca tacaagctga   6900

acaagcgctc catacttgca cgctctctat atacacagtt aaattacata tccatagtct   6960

aacctctaac agttaatctt ctggtaagcc tcccagccag ccttctggta tcgcttggcc   7020

tcctcaatag gatctcggtt ctggccgtac agacctcggc cgacaattat gatatccgtt   7080

ccggtagaca tgacatcctc aacagttcgg tactgctgtc cgagagcgtc tcccttgtcg   7140

tcaagaccca ccccgggggt cagaataagc cagtcctcag agtcgccctt aggtcggttc   7200

tgggcaatga agccaaccac aaactcgggg tcggatcggg caagctcaat ggtctgcttg   7260

gagtactcgc cagtggccag agagcccttg caagacagct cggccagcat gagcagacct   7320

ctggccagct tctcgttggg agaggggact aggaactcct tgtactggga gttctcgtag   7380

tcagagacgt cctccttctt ctgttcagag acagtttcct cggcaccagc tcgcaggcca   7440

gcaatgattc cggttccggg tacaccgtgg gcgttggtga tatcggacca ctcggcgatt   7500

cggtgacacc ggtactggtg cttgacagtg ttgccaatat ctgcgaactt tctgtcctcg   7560

aacaggaaga aaccgtgctt aagagcaagt tccttgaggg ggagcacagt gccggcgtag   7620

gtgaagtcgt caatgatgtc gatatgggtt ttgatcatgc acacataagg tccgacctta   7680

tcggcaagct caatgagctc cttggtggtg gtaacatcca gagaagcaca caggttggtt   7740

ttcttggctg ccacgagctt gagcactcga gcggcaaagg cggacttgtg gacgttagct   7800

cgagcttcgt aggagggcat tttggtggtg aagaggagac tgaaataaat ttagtctgca   7860

gaacttttta tcggaacctt atctggggca gtgaagtata tgttatggta atagttacga   7920

gttagttgaa cttatagata gactggacta tacggctatc ggtccaaatt agaaagaacg   7980

tcaatggctc tctgggcgtc gcctttgccg acaaaaatgt gatcatgatg aaagccagca   8040

atgacgttgc agctgatatt gttgtcggcc aaccgcgccg aaaacgcagc tgtcagaccc   8100

acagcctcca acgaagaatg tatcgtcaaa gtgatccaag cacactcata gttggagtcg   8160

tactccaaag gcggcaatga cgagtcagac agatactcgt cgaccttttc cttgggaacc   8220

accaccgtca gcccttctga ctcacgtatt gtagccaccg acacaggcaa cagtccgtgg   8280

atagcagaat atgtcttgtc ggtccatttc tcaccaactt taggcgtcaa gtgaatgttg   8340

cagaagaagt atgtgccttc attgagaatc ggtgttgctg atttcaataa agtcttgaga   8400

tcagtttggc cagtcatgtt gtggggggta attggattga gttatcgcct acagtctgta   8460

caggtatact cgctgcccac tttatacttt ttgattccgc tgcacttgaa gcaatgtcgt   8520

ttaccaaaag tgagaatgct ccacagaaca caccccaggg tatggttgag caaaaaataa   8580

acactccgat acggggaatc gaaccccggt ctccacggtt ctcaagaagt attcttgatg   8640

agagcgtatc gatcgaggaa gaggacaagc ggctgcttct taagtttgtg acatcagtat   8700

ccaaggcacc attgcaagga ttcaaggctt tgaacccgtc atttgccatt cgtaacgctg   8760

gtagacaggt tgatcggttc cctacggcct ccacctgtgt caatcttctc aagctgcctg   8820

actatcagga cattgatcaa cttcggaaga aacttttgta tgccattcga tcacatgctg   8880

gtttcgattt gtcttagagg aacgcatata cagtaatcat agagaataaa cgatattcat   8940

ttattaaagt agatagttga ggtagaagtt gtaaagagtg ataaatagcg gccgctcact   9000

gaatcttttt ggctcccttg tgctttcgga cgatgtaggt ctgcacgtag aagttgagga   9060

acagacacag gacagtacca acgtagaagt agttgaaaaa ccagccaaac attctcattc   9120

catcttgtcg gtagcaggga atgttccggt acttccagac gatgtagaag ccaacgttga   9180

actgaatgat ctgcatagaa gtaatcaggg acttgggcat agggaacttg agcttgatca   9240

gtcgggtcca atagtagccg tacatgatcc agtgaatgaa gccgttgagc agcacaaaga   9300

tccaaacggc ttcgtttcgg tagttgtaga acagccacat gtccatagga gctccgagat   9360

ggtgaaagaa ctgcaaccag gtcagaggct tgcccatgag gggcagatag aaggagtcaa   9420

tgtactcgag gaacttgctg aggtagaaca gctgagtggt gattcggaag acattgttgt   9480

cgaaagcctt ctcgcagttg tcggacatga caccaatggt gtacatggcg taggccatag   9540

agaggaagga gcccagcgag tagatggaca tgagcaggtt gtagttggtg aacacaaact   9600

tcattcgaga ctgacccttg ggtccgagag gaccaagggt gaacttcagg atgacgaagg   9660

cgatggagag gtacagcacc tcgcagtgcg aggcatcaga ccagagctga gcatagtcga   9720

ccttgggaag aacctcctgg ccaatggaga cgatttcgtt cacgacctcc atggttgatg   9780

tgtgtttaat tcaagaatga atatagagaa gagaagaaga aaaaagattc aattgagccg   9840

gcgatgcaga cccttatata aatgttgcct tggacagacg gagcaagccc gcccaaacct   9900

acgttcggta taatatgtta agctttttaa cacaaaggtt tggcttgggg taacctgatg   9960

tggtgcaaaa gaccgggcgt tggcgagcca ttgcgcgggc gaatggggcc gtgactcgtc  10020

tcaaattcga gggcgtgcct caattcgtgc ccccgtggct ttttcccgcc gtttccgccc  10080

cgtttgcacc actgcagccg cttctttggt tcggacacct tgctgcgagc taggtgcctt  10140

gtgctactta aaaagtggcc tcccaacacc aacatgacat gagtgcgtgg gccaagacac  10200

gttggcgggg tcgcagtcgg ctcaatggcc cggaaaaaac gctgctggag ctggttcgga  10260

cgcagtccgc cgcggcgtat ggatatccgc aaggttccat agcgccattg ccctccgtcg  10320

gcgtctatcc cgcaacctct aaatagagcg ggaatataac ccaagcttct tttttttcct  10380

ttaacacgca cacccccaac tatcatgttg ctgctgctgt ttgactctac tctgtggagg  10440

ggtgctccca cccaacccaa cctacaggtg gatccggcgc tgtgattggc tgataagtct  10500

cctatccgga ctaattctga ccaatgggac atgcgcgcag gacccaaatg ccgcaattac  10560

gtaaccccaa cgaaatgcct acccctcttt ggagcccagc ggccccaaat ccccccaagc  10620

agcccggttc taccggcttc catctccaag cacaagcagc ccggttctac cggcttccat  10680

ctccaagcac ccctttctcc acaccccaca aaaagacccg tgcaggacat cctactgcgt  10740

gtttaaacac cactaaaacc ccacaaaata tatcttaccg aatatacaga tctactatag  10800

aggaacaatt gccccggaga agacggccag gccgcctaga tgacaaattc aacaactcac  10860

agctgacttt ctgccattgc cactaggggg gggccttttt atatggccaa gccaagctct  10920

ccacgtcggt tgggctgcac ccaacaataa atgggtaggg ttgcaccaac aaagggatgg  10980

gatggggggt agaagatacg aggataacgg ggctcaatgg cacaaataag aacgaatact  11040

gccattaaga ctcgtgatcc agcgactgac accattgcat catctaaggg cctcaaaact  11100

acctcggaac tgctgcgctg atctggacac cacagaggtt ccgagcactt taggttgcac  11160

caaatgtccc accaggtgca ggcagaaaac gctggaacag cgtgtacagt ttgtcttaac  11220

aaaaagtgag ggcgctgagg tcgagcaggg tggtgtgact tgttatagcc tttagagctg  11280

cgaaagcgcg tatggatttg gctcatcagg ccagattgag ggtctgtgga cacatgtcat  11340

gttagtgtac ttcaatcgcc ccctggatat agccccgaca ataggccgtg gcctcatttt  11400

tttgccttcc gcacatttcc attgctcggt acccacacct tgcttctcct gcacttgcca  11460

accttaatac tggtttacat tgaccaacat cttacaagcg gggggcttgt ctagggtata  11520

tataaacagt ggctctccca atcggttgcc agtctctttt ttcctttctt tccccacaga  11580

ttcgaaatct aaactacaca tcacacaatg cctgttactg acgtccttaa gcgaaagtcc  11640

ggtgtcatcg tcggcgacga tgtccgagcc gtgagtatcc acgacaagat cagtgtcgag  11700

acgacgcgtt ttgtgtaatg acacaatccg aaagtcgcta gcaacacaca ctctctacac  11760

aaactaaccc agctctccat ggtgaaggct tctcgacagg ctctgcccct cgtcatcgac  11820

ggaaaggtgt acgacgtctc cgcttgggtg aacttccacc ctggtggagc tgaaatcatt  11880

gagaactacc agggacgaga tgctactgac gccttcatgg ttatgcactc tcaggaagcc  11940

ttcgacaagc tcaagcgaat gcccaagatc aaccaggctt ccgagctgcc tccccaggct  12000

gccgtcaacg aagctcagga ggatttccga aagctccgag aagagctgat cgccactggc  12060

atgtttgacg cctctcccct ctggtactcg tacaagatct tgaccaccct gggtcttggc  12120

gtgcttgcct tcttcatgct ggtccagtac cacctgtact tcattggtgc tctcgtgctc  12180

ggtatgcact accagcaaat gggatggctg tctcatgaca tctgccacca ccagaccttc  12240

aagaaccgaa actggaataa cgtcctgggt ctggtctttg gcaacggact ccagggcttc  12300

tccgtgacct ggtggaagga cagacacaac gcccatcatt ctgctaccaa cgttcagggt  12360

cacgatcccg acattgataa cctgcctctg ctcgcctggt ccgaggacga tgtcactcga  12420

gcttctccca tctcccgaaa gctcattcag ttccaacagt actatttcct ggtcatctgt  12480

attctcctgc gattcatctg gtgtttccag tctgtgctga ccgttcgatc cctcaaggac  12540

cgagacaacc agttctaccg atctcagtac aagaaagagg ccattggact cgctctgcac  12600

tggactctca agaccctgtt ccacctcttc tttatgccct ccatcctgac ctcgatgctg  12660

gtgttctttg tttccgagct cgtcggtggc ttcggaattg ccatcgtggt cttcatgaac  12720

cactaccctc tggagaagat cggtgattcc gtctgggacg gacatggctt ctctgtgggt  12780

cagatccatg agaccatgaa cattcgacga ggcatcatta ctgactggtt ctttggaggc  12840

ctgaactacc agatcgagca ccatctctgg cccaccctgc ctcgacacaa cctcactgcc  12900

gtttcctacc aggtggaaca gctgtgccag aagcacaacc tcccctaccg aaaccctctg  12960

ccccatgaag gtctcgtcat cctgctccga tacctgtccc agttcgctcg aatggccgag  13020

aagcagcccg gtgccaaggc tcagtaagcg gccgcatgag aagataaata tataaataca  13080

ttgagatatt aaatgcgcta gattagagag cctcatactg ctcggagaga agccaagacg  13140

agtactcaaa ggggattaca ccatccatat ccacagacac aagctgggga aaggttctat  13200

atacactttc cggaataccg tagtttccga tgttatcaat gggggcagcc aggatttcag  13260

gcacttcggt gtctcggggt gaaatggcgt tcttggcctc catcaagtcg taccatgtct  13320

tcatttgcct gtcaaagtaa aacagaagca gatgaagaat gaacttgaag tgaaggaatt  13380

taaatagttg gagcaaggga gaaatgtaga gtgtgaaaga ctcactatgg tccgggctta  13440

tctcgaccaa tagccaaagt ctggagtttc tgagagaaaa aggcaagata cgtatgtaac  13500

aaagcgacgc atggtacaat aataccggag gcatgtatca tagagagtta gtggttcgat  13560

gatggcactg gtgcctggta tgactttata cggctgacta catatttgtc ctcagacata  13620

caattacagt caagcactta cccttggaca tctgtaggta ccccccggcc aagacgatct  13680

cagcgtgtcg tatgtcggat tggcgtagct ccctcgctcg tcaattggct cccatctact  13740

ttcttctgct tggctacacc cagcatgtct gctatggctc gttttcgtgc cttatctatc  13800

ctcccagtat taccaactct aaatgacatg atgtgattgg gtctacactt tcatatcaga  13860

gataaggagt agcacagttg cataaaaagc ccaactctaa tcagcttctt cctttcttgt  13920

aattagtaca aaggtgatta gcgaaatctg gaagcttagt tggccctaaa aaaatcaaaa  13980

aaagcaaaaa acgaaaaacg aaaaaccaca gttttgagaa cagggaggta acgaaggatc  14040

gtatatatat atatatatat atatacccac ggatcccgag accggccttt gattcttccc  14100

tacaaccaac cattctcacc accctaattc acaaccatgg gcgtattcat taaacaggag  14160

cagcttccgg ctctcaagaa gtacaagtac tccgccgagg atcactcgtt catctccaac  14220

aacattctgc gccccttctg gcgacagttt gtcaaaatct tccctctgtg gatggccccc  14280

aacatggtga ctctgctggg cttcttcttt gtcattgtga acttcatcac catgctcatt  14340

gttgatccca cccacgaccg cgagcctccc agatgggtct acctcaccta cgctctgggt  14400

ctgttccttt accagacatt tgatgcctgt gacggatccc atgcccgacg aactggccag  14460

agtggacccc ttggagagct gtttgaccac tgtgtcgacg ccatgaatac ctctctgatt  14520

ctcacggtgg tggtgtccac cacccatatg ggatataaca tgaagctact gattgtgcag  14580

attgccgctc tcggaaactt ctacctgtcg acctgggaga cctaccatac cggaactctg  14640

tacctttctg gcttctctgg tcctgttgaa ggtatcttga ttctggtggc tcttttcgtc  14700

ctcaccttct tcactggtcc caacgtgtac gctctgaccg tctacgaggc tcttcccgag  14760

tccatcactt cgctgctgcc tgccagcttc ctggacgtca ccatcaccca gatctacatt  14820

ggattcggag tgctgggcat ggtgttcaac atctacggcg cctgcggaaa cgtgatcaag  14880

tactacaaca acaagggcaa gagcgctctc cccgccattc tcggaatcgc cccctttggc  14940

atcttctacg tcggcgtctt tgcctgggcc catgttgctc ctctgcttct ctccaagtac  15000

gccatcgtct atctgtttgc cattggggct gcctttgcca tgcaagtcgg ccagatgatt  15060

cttgcccatc tcgtgcttgc tccctttccc cactggaacg tgctgctctt cttccccttt  15120

gtgggactgg cagtgcacta cattgcaccc gtgtttggct gggacgccga tatcgtgtcg  15180

gttaacactc tcttcacctg ttttggcgcc accctctcca tttacgcctt ctttgtgctt  15240

gagatcatcg acgagatcac caactacctc gatatctggt gtctgcgaat caagtaccct  15300

caggagaaga agaccgaata agcggccgca tggagcgtgt gttctgagtc gatgttttct  15360

atggagttgt gagtgttagt agacatgatg ggtttatata tgatgaatga atagatgtga  15420

ttttgatttg cacgatggaa ttgagaactt tgtaaacgta catgggaatg tatgaatgtg  15480

ggggttttgt gactggataa ctgacggtca gtggacgccg ttgttcaaat atccaagaga  15540

tgcgagaaac tttgggtcaa gtgaacatgt cctctctgtt caagtaaacc atcaactatg  15600

ggtagtatat ttagtaagga caagagttga gattctttgg agtcctagaa acgtattttc  15660

gcgttccaag atcaaattag tagagtaata cgggcacggg aatccattca tagtctcaat  15720

cctgcaggtg agttaattaa tcgagcttgg cgtaatcatg gtcatagctg tttcctgtgt  15780

gaaattgtta tccgctcaca attccacaca ac                                15812


<210>  132
<211>  15877
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZKL1-2SP98C

<400>  132
aaatgatgtc gacgcagtag gatgtcctgc acgggtcttt ttgtggggtg tggagaaagg     60

ggtgcttgga tcgatggaag ccggtagaac cgggctgctt gtgcttggag atggaagccg    120

gtagaaccgg gctgcttggg gggatttggg gccgctgggc tccaaagagg ggtaggcatt    180

tcgttggggt tacgtaattg cggcatttgg gtcctgcgcg catgtcccat tggtcagaat    240

tagtccggat aggagactta tcagccaatc acagcgccgg atccacctgt aggttgggtt    300

gggtgggagc acccctccac agagtagagt caaacagcag cagcaacatg atagttgggg    360

gtgtgcgtgt taaaggaaaa aaaagaagct tgggttatat tcccgctcta tttagaggtt    420

gcgggataga cgccgacgga gggcaatggc gctatggaac cttgcggata tccatacgcc    480

gcggcggact gcgtccgaac cagctccagc agcgtttttt ccgggccatt gagccgactg    540

cgaccccgcc aacgtgtctt ggcccacgca ctcatgtcat gttggtgttg ggaggccact    600

ttttaagtag cacaaggcac ctagctcgca gcaaggtgtc cgaaccaaag aagcggctgc    660

agtggtgcaa acggggcgga aacggcggga aaaagccacg ggggcacgaa ttgaggcacg    720

ccctcgaatt tgagacgagt cacggcccca ttcgcccgcg caatggctcg ccaacgcccg    780

gtcttttgca ccacatcagg ttaccccaag ccaaaccttt gtgttaaaaa gcttaacata    840

ttataccgaa cgtaggtttg ggcgggcttg ctccgtctgt ccaaggcaac atttatataa    900

gggtctgcat cgccggctca attgaatctt ttttcttctt ctcttctcta tattcattct    960

tgaattaaac acacatcaac catgggcgta ttcattaaac aggagcagct tccggctctc   1020

aagaagtaca agtactccgc cgaggatcac tcgttcatct ccaacaacat tctgcgcccc   1080

ttctggcgac agtttgtcaa aatcttccct ctgtggatgg cccccaacat ggtgactctg   1140

ctgggcttct tctttgtcat tgtgaacttc atcaccatgc tcattgttga tcccacccac   1200

gaccgcgagc ctcccagatg ggtctacctc acctacgctc tgggtctgtt cctttaccag   1260

acatttgatg cctgtgacgg atcccatgcc cgacgaactg gccagagtgg accccttgga   1320

gagctgtttg accactgtgt cgacgccatg aatacctctc tgattctcac ggtggtggtg   1380

tccaccaccc atatgggata taacatgaag ctactgattg tgcagattgc cgctctcgga   1440

aacttctacc tgtcgacctg ggagacctac cataccggaa ctctgtacct ttctggcttc   1500

tctggtcctg ttgaaggtat cttgattctg gtggctcttt tcgtcctcac cttcttcact   1560

ggtcccaacg tgtacgctct gaccgtctac gaggctcttc ccgagtccat cacttcgctg   1620

ctgcctgcca gcttcctgga cgtcaccatc acccagatct acattggatt cggagtgctg   1680

ggcatggtgt tcaacatcta cggcgcctgc ggaaacgtga tcaagtacta caacaacaag   1740

ggcaagagcg ctctccccgc cattctcgga atcgccccct ttggcatctt ctacgtcggc   1800

gtctttgcct gggcccatgt tgctcctctg cttctctcca agtacgccat cgtctatctg   1860

tttgccattg gggctgcctt tgccatgcaa gtcggccaga tgattcttgc ccatctcgtg   1920

cttgctccct ttccccactg gaacgtgctg ctcttcttcc cctttgtggg actggcagtg   1980

cactacattg cacccgtgtt tggctgggac gccgatatcg tgtcggttaa cactctcttc   2040

acctgttttg gcgccaccct ctccatttac gccttctttg tgcttgagat catcgacgag   2100

atcaccaact acctcgatat ctggtgtctg cgaatcaagt accctcagga gaagaagacc   2160

gaataagcgg ccgcatggag cgtgtgttct gagtcgatgt tttctatgga gttgtgagtg   2220

ttagtagaca tgatgggttt atatatgatg aatgaataga tgtgattttg atttgcacga   2280

tggaattgag aactttgtaa acgtacatgg gaatgtatga atgtgggggt tttgtgactg   2340

gataactgac ggtcagtgga cgccgttgtt caaatatcca agagatgcga gaaactttgg   2400

gtcaagtgaa catgtcctct ctgttcaagt aaaccatcaa ctatgggtag tatatttagt   2460

aaggacaaga gttgagattc tttggagtcc tagaaacgta ttttcgcgtt ccaagatcaa   2520

attagtagag taatacgggc acgggaatcc attcatagtc tcaatcctgc aggtgagtta   2580

attaatcgag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc   2640

tcacaattcc acacaacgta cgatagttag tagacaacaa tcagaacatc tccctcctta   2700

tataatcaca caggccagaa cgcgctaaac taaagcgctt tggacactat gttacattgg   2760

cattgattga actgaaacca cagtctccct cgcctgaatc gagcaatgga tgttgtcgga   2820

agtcaacttc actagaagag cggttctatg ccttgtcaag atcatatcat aaactcactc   2880

tgtattaccc catctataga acacttgtta tgaatgggcg gaaacattcc gctatatgca   2940

cctttccaca ctaatgcaaa gatgtgcatc ttcaacgggt agtaagactg gttccgactt   3000

ccgttgcatg gagagcaatg acctcgataa tgcgaacatc ccccacatat acactcttac   3060

acaggccaat ataatctgtg catttactaa atatttaagt ctatgcacct gcttgatgaa   3120

aagcggcacg gatggtatca tctagtttcc gccaatccaa gaaccaactg tgttggcagt   3180

ggtgtagccc atggcacaca gaccaaagat gaaaatacag acatcggcgg ttcgagccgt   3240

ggtgcctcga gcaacaccct tgtaatgcaa aagaggaggg taaatgtaca ccagaggcac   3300

acatgcaaac gatccggtga gagcgacgaa ccgatcgaga tcgtcggcac ctccccatgc   3360

aacaaaggcg gtgacaaaca caaggaagaa ccggaaaatg ttcttctgcc acttgatggt   3420

agagttgtac ttgcctgatc gggtgaagag accattctcg atgattcgga tggcgcgcca   3480

gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc   3540

cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc   3600

tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat   3660

gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt   3720

ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg   3780

aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc   3840

tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt   3900

ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa   3960

gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta   4020

tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa   4080

caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa   4140

ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt   4200

cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt   4260

ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat   4320

cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat   4380

gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc   4440

aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc   4500

acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta   4560

gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga   4620

cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg   4680

cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc   4740

tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat   4800

cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag   4860

gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat   4920

cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa   4980

ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa   5040

gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga   5100

taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg   5160

gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc   5220

acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg   5280

aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact   5340

cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat   5400

atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt   5460

gccacctgat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcagga   5520

aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt   5580

ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat   5640

agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa   5700

cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta   5760

atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc   5820

ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc   5880

gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac   5940

acccgccgcg cttaatgcgc cgctacaggg cgcgtccatt cgccattcag gctgcgcaac   6000

tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga   6060

tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa   6120

acgacggcca gtgaattgta atacgactca ctatagggcg aattgggccc gacgtcgcat   6180

gcttagaagt gaggattaca agaagcctct ggatatcaat gatgaacgta ctcagcggct   6240

ggtcaagcat ttcgaccgtc gaatcgacga ggtgttcacc tttgacaagc gagggttccc   6300

aattgatcac gttctcgagt tgttcaaatc ttctctcaac atctctctgc atgaactatc   6360

tctgttgacg aacgtgtcac ccactgttcc tcgaacgccc ttctccgagt ttggtctgaa   6420

catcttcgat ctcaaactga cccccgcagt gatcaatagt gccatgccac tgccgatgcg   6480

gtgcgaacat ccctggaggg attctcggag ctctacacaa tgcagattct gtcgtcgagt   6540

actctctacc ttgctcgaat gacttattgt gctactactg cactcatgct tcgatcatgt   6600

gccctactgc accccaaatt tggtgatctg attgagacag agtaccctct tcagctgatt   6660

cagaagatca tcagcaacat gaatgatgtg gttgaccagg caggctgttg tagtcacgtc   6720

cttcacttca agttcattct tcatctgctt ctgttttact ttgacaggca aatgaagaca   6780

tggtacgact tgatggaggc caagaacgcc atttcacccc gagacaccga agtgcctgaa   6840

atcctggctg cccccattga taacatcgga aactacggta ttccggaaag tgtatataga   6900

acctttcccc agcttgtgtc tgtggatatg gatggtgtaa tccccttaat taactcacct   6960

gcaggattga gactatgaat ggattcccgt gcccgtatta ctctactaat ttgatcttgg   7020

aacgcgaaaa tacgtttcta ggactccaaa gaatctcaac tcttgtcctt actaaatata   7080

ctacccatag ttgatggttt acttgaacag agaggacatg ttcacttgac ccaaagtttc   7140

tcgcatctct tggatatttg aacaacggcg tccactgacc gtcagttatc cagtcacaaa   7200

acccccacat tcatacattc ccatgtacgt ttacaaagtt ctcaattcca tcgtgcaaat   7260

caaaatcaca tctattcatt catcatatat aaacccatca tgtctactaa cactcacaac   7320

tccatagaaa acatcgactc agaacacacg ctccatgcgg ccgcttaggc aacgggcttg   7380

atgacagcgg gaggagtgcc cacattgttt cggtttcgaa agaacaggac acccttgcca   7440

gctccctcgg caccagcgga gggttcaacc cactggcaca ttcgtgcaga tcggtacatg   7500

gctcgaatga atcctcgagg accgtcctgg acatcagctc gatagtgctt gcccatgata   7560

ggtttgatgg cctcggtagc ttcgtccgca ttgtagaagg gaatggaaga gacgtagtga   7620

tgcaggacgt gagtctcgat aatgccgtgg agcagatgac gtccaatgaa gcccatctct   7680

cggtcgatgg ttgcagcggc acctcgcaca aagttccact cgtcgttggt gtagtgggga   7740

agagtaggat ctgtgtgctg cagaaaggta atggcgacga gccagtggtt aacccacaag   7800

tagggaacga agtaccagat ggccatgttg tagaatccga acttctgaac gagaaagtac   7860

agagcggtgg ccataagacc aatgccaatg tcggagagca cgatgagctt ggcgtcgctg   7920

ttctcgtaca gaggagatcg gggatcgaaa tggttaactc caccgccaag accgttgtgc   7980

tttcccttgc ctcgaccctc tcgctgccgc tcatggtagt tgtgtccagt aacgttggta   8040

atgagatagt tgggccaacc gaccagttgc tgaagcacaa gcatgagcag ggtgaaagca   8100

ggagtttcct cggtaagatg ggcgagttcg tgggtcatct tgccgagtcg agtagcttgc   8160

tgctctcggg ttcgaggaac gaagaccatg tctcgctcca tgtttccagt ggccttgtga   8220

tgcttccggt gggagatttg ccagctgaag tagggaacaa gcagggaaga gtgaagcacc   8280

cagccagtaa tgtcgttgat gattcgggaa tcggagaaag caccatgtcc acactcgtgg   8340

gcaatgaccc acagtccagt accgaagagt ccctgaagaa cggtgtacac agcccacaga   8400

ccggctcgag caggagtgga gggaatgtac tcgggtgtca caaagttgta ccagatgctg   8460

aaagtggtag tcaggaggac aatgtctcga agaatgtagc cgtatccctt gagagcagat   8520

cgcttgaagc agtgcttggg aatagcgttg tagatgtcct tgatggtgaa gtcgggaact   8580

tcgaactggt tgccgtaggt atccagcatg acaccgtact cggacttggg cttggcaatg   8640

tccacctcgg acatggaaga cagcgatgta gaggaggccg agtgtctggg agaatcggag   8700

ggagagacgg cagcagactc cgagtcggtc acagtggtgg aagtgacggt tcgtcggagg   8760

gcagggttct gcttgggcag agccgaggtg gaggccatgg ccattgctgt agatatgtct   8820

tgtgtgtaag ggggttgggg tggttgtttg tgttcttgac ttttgtgtta gcaagggaag   8880

acgggcaaaa aagtgagtgt ggttgggagg gagagacgag ccttatatat aatgcttgtt   8940

tgtgtttgtg caagtggacg ccgaaacggg caggagccaa actaaacaag gcagacaatg   9000

cgagcttaat tggattgcct gatgggcagg ggttagggct cgatcaatgg gggtgcgaag   9060

tgacaaaatt gggaattagg ttcgcaagca aggctgacaa gactttggcc caaacatttg   9120

tacgcggtgg acaacaggag ccacccatcg tctgtcacgg gctagccggt cgtgcgtcct   9180

gtcaggctcc acctaggctc catgccactc catacaatcc cactagtgta ccgctaggcc   9240

gcttttagct cccatctaag acccccccaa aacctccact gtacagtgca ctgtactgtg   9300

tggcgatcaa gggcaaggga aaaaaggcgc aaacatgcac gcatggaatg acgtaggtaa   9360

ggcgttacta gactgaaaag tggcacattt cggcgtgcca aagggtccta ggtgcgtttc   9420

gcgagctggg cgccaggcca agccgctcca aaacgcctct ccgactccct ccagcggcct   9480

ccatatcccc atccctctcc acagcaatgt tgttaagcct tgcaaacgaa aaaatagaaa   9540

ggctaataag cttccaatat tgtggtgtac gctgcataac gcaacaatga gcgccaaaca   9600

acacacacac acagcacaca gcagcattaa ccacgatgaa cagcatgaat tcctttacct   9660

gcaggataac ttcgtataat gtatgctata cgaagttatg atctctctct tgagcttttc   9720

cataacaagt tcttctgcct ccaggaagtc catgggtggt ttgatcatgg ttttggtgta   9780

gtggtagtgc agtggtggta ttgtgactgg ggatgtagtt gagaataagt catacacaag   9840

tcagctttct tcgagcctca tataagtata agtagttcaa cgtattagca ctgtacccag   9900

catctccgta tcgagaaaca caacaacatg ccccattgga cagatcatgc ggatacacag   9960

gttgtgcagt atcatacata ctcgatcaga caggtcgtct gaccatcata caagctgaac  10020

aagcgctcca tacttgcacg ctctctatat acacagttaa attacatatc catagtctaa  10080

cctctaacag ttaatcttct ggtaagcctc ccagccagcc ttctggtatc gcttggcctc  10140

ctcaatagga tctcggttct ggccgtacag acctcggccg acaattatga tatccgttcc  10200

ggtagacatg acatcctcaa cagttcggta ctgctgtccg agagcgtctc ccttgtcgtc  10260

aagacccacc ccgggggtca gaataagcca gtcctcagag tcgcccttag gtcggttctg  10320

ggcaatgaag ccaaccacaa actcggggtc ggatcgggca agctcaatgg tctgcttgga  10380

gtactcgcca gtggccagag agcccttgca agacagctcg gccagcatga gcagacctct  10440

ggccagcttc tcgttgggag aggggactag gaactccttg tactgggagt tctcgtagtc  10500

agagacgtcc tccttcttct gttcagagac agtttcctcg gcaccagctc gcaggccagc  10560

aatgattccg gttccgggta caccgtgggc gttggtgata tcggaccact cggcgattcg  10620

gtgacaccgg tactggtgct tgacagtgtt gccaatatct gcgaactttc tgtcctcgaa  10680

caggaagaaa ccgtgcttaa gagcaagttc cttgaggggg agcacagtgc cggcgtaggt  10740

gaagtcgtca atgatgtcga tatgggtttt gatcatgcac acataaggtc cgaccttatc  10800

ggcaagctca atgagctcct tggtggtggt aacatccaga gaagcacaca ggttggtttt  10860

cttggctgcc acgagcttga gcactcgagc ggcaaaggcg gacttgtgga cgttagctcg  10920

agcttcgtag gagggcattt tggtggtgaa gaggagactg aaataaattt agtctgcaga  10980

actttttatc ggaaccttat ctggggcagt gaagtatatg ttatggtaat agttacgagt  11040

tagttgaact tatagataga ctggactata cggctatcgg tccaaattag aaagaacgtc  11100

aatggctctc tgggcgtcgc ctttgccgac aaaaatgtga tcatgatgaa agccagcaat  11160

gacgttgcag ctgatattgt tgtcggccaa ccgcgccgaa aacgcagctg tcagacccac  11220

agcctccaac gaagaatgta tcgtcaaagt gatccaagca cactcatagt tggagtcgta  11280

ctccaaaggc ggcaatgacg agtcagacag atactcgtcg acgcgataac ttcgtataat  11340

gtatgctata cgaagttatc gtacgatagt tagtagacaa caatcgatcg aggaagagga  11400

caagcggctg cttcttaagt ttgtgacatc agtatccaag gcaccattgc aaggattcaa  11460

ggctttgaac ccgtcatttg ccattcgtaa cgctggtaga caggttgatc ggttccctac  11520

ggcctccacc tgtgtcaatc ttctcaagct gcctgactat caggacattg atcaacttcg  11580

gaagaaactt ttgtatgcca ttcgatcaca tgctggtttc gatttgtctt agaggaacgc  11640

atatacagta atcatagaga ataaacgata ttcatttatt aaagtagata gttgaggtag  11700

aagttgtaaa gagtgataaa tagcggccgc tcactgaatc tttttggctc ccttgtgctt  11760

tcggacgatg taggtctgca cgtagaagtt gaggaacaga cacaggacag taccaacgta  11820

gaagtagttg aaaaaccagc caaacattct cattccatct tgtcggtagc agggaatgtt  11880

ccggtacttc cagacgatgt agaagccaac gttgaactga atgatctgca tagaagtaat  11940

cagggacttg ggcataggga acttgagctt gatcagtcgg gtccaatagt agccgtacat  12000

gatccagtga atgaagccgt tgagcagcac aaagatccaa acggcttcgt ttcggtagtt  12060

gtagaacagc cacatgtcca taggagctcc gagatggtga aagaactgca accaggtcag  12120

aggcttgccc atgaggggca gatagaagga gtcaatgtac tcgaggaact tgctgaggta  12180

gaacagctga gtggtgattc ggaagacatt gttgtcgaaa gccttctcgc agttgtcgga  12240

catgacacca atggtgtaca tggcgtaggc catagagagg aaggagccca gcgagtagat  12300

ggacatgagc aggttgtagt tggtgaacac aaacttcatt cgagactgac ccttgggtcc  12360

gagaggacca agggtgaact tcaggatgac gaaggcgatg gagaggtaca gcacctcgca  12420

gtgcgaggca tcagaccaga gctgagcata gtcgaccttg ggaagaacct cctggccaat  12480

ggagacgatt tcgttcacga cctccatggt tgtgaattag ggtggtgaga atggttggtt  12540

gtagggaaga atcaaaggcc ggtctcggga tccgtgggta tatatatata tatatatata  12600

tacgatcctt cgttacctcc ctgttctcaa aactgtggtt tttcgttttt cgttttttgc  12660

tttttttgat ttttttaggg ccaactaagc ttccagattt cgctaatcac ctttgtacta  12720

attacaagaa aggaagaagc tgattagagt tgggcttttt atgcaactgt gctactcctt  12780

atctctgata tgaaagtgta gacccaatca catcatgtca tttagagttg gtaatactgg  12840

gaggatagat aaggcacgaa aacgagccat agcagacatg ctgggtgtag ccaagcagaa  12900

gaaagtagat gggagccaat tgacgagcga gggagctacg ccaatccgac atacgacacg  12960

ctgagatcgt cttggccggg gggtacctac agatgtccaa gggtaagtgc ttgactgtaa  13020

ttgtatgtct gaggacaaat atgtagtcag ccgtataaag tcataccagg caccagtgcc  13080

atcatcgaac cactaactct ctatgataca tgcctccggt attattgtac catgcgtcgc  13140

tttgttacat acgtatcttg cctttttctc tcagaaactc cagactttgg ctattggtcg  13200

agataagccc ggaccatagt gagtctttca cactctgttt aaacaccact aaaaccccac  13260

aaaatatatc ttaccgaata tacagatcta ctatagagga acaattgccc cggagaagac  13320

ggccaggccg cctagatgac aaattcaaca actcacagct gactttctgc cattgccact  13380

aggggggggc ctttttatat ggccaagcca agctctccac gtcggttggg ctgcacccaa  13440

caataaatgg gtagggttgc accaacaaag ggatgggatg gggggtagaa gatacgagga  13500

taacggggct caatggcaca aataagaacg aatactgcca ttaagactcg tgatccagcg  13560

actgacacca ttgcatcatc taagggcctc aaaactacct cggaactgct gcgctgatct  13620

ggacaccaca gaggttccga gcactttagg ttgcaccaaa tgtcccacca ggtgcaggca  13680

gaaaacgctg gaacagcgtg tacagtttgt cttaacaaaa agtgagggcg ctgaggtcga  13740

gcagggtggt gtgacttgtt atagccttta gagctgcgaa agcgcgtatg gatttggctc  13800

atcaggccag attgagggtc tgtggacaca tgtcatgtta gtgtacttca atcgccccct  13860

ggatatagcc ccgacaatag gccgtggcct catttttttg ccttccgcac atttccattg  13920

ctcggtaccc acaccttgct tctcctgcac ttgccaacct taatactggt ttacattgac  13980

caacatctta caagcggggg gcttgtctag ggtatatata aacagtggct ctcccaatcg  14040

gttgccagtc tcttttttcc tttctttccc cacagattcg aaatctaaac tacacatcac  14100

acaatgcctg ttactgacgt ccttaagcga aagtccggtg tcatcgtcgg cgacgatgtc  14160

cgagccgtga gtatccacga caagatcagt gtcgagacga cgcgttttgt gtaatgacac  14220

aatccgaaag tcgctagcaa cacacactct ctacacaaac taacccagct ctccatggtg  14280

aaggcttctc gacaggctct gcccctcgtc atcgacggaa aggtgtacga cgtctccgct  14340

tgggtgaact tccaccctgg tggagctgaa atcattgaga actaccaggg acgagatgct  14400

actgacgcct tcatggttat gcactctcag gaagccttcg acaagctcaa gcgaatgccc  14460

aagatcaacc aggcttccga gctgcctccc caggctgccg tcaacgaagc tcaggaggat  14520

ttccgaaagc tccgagaaga gctgatcgcc actggcatgt ttgacgcctc tcccctctgg  14580

tactcgtaca agatcttgac caccctgggt cttggcgtgc ttgccttctt catgctggtc  14640

cagtaccacc tgtacttcat tggtgctctc gtgctcggta tgcactacca gcaaatggga  14700

tggctgtctc atgacatctg ccaccaccag accttcaaga accgaaactg gaataacgtc  14760

ctgggtctgg tctttggcaa cggactccag ggcttctccg tgacctggtg gaaggacaga  14820

cacaacgccc atcattctgc taccaacgtt cagggtcacg atcccgacat tgataacctg  14880

cctctgctcg cctggtccga ggacgatgtc actcgagctt ctcccatctc ccgaaagctc  14940

attcagttcc aacagtacta tttcctggtc atctgtattc tcctgcgatt catctggtgt  15000

ttccagtctg tgctgaccgt tcgatccctc aaggaccgag acaaccagtt ctaccgatct  15060

cagtacaaga aagaggccat tggactcgct ctgcactgga ctctcaagac cctgttccac  15120

ctcttcttta tgccctccat cctgacctcg atgctggtgt tctttgtttc cgagctcgtc  15180

ggtggcttcg gaattgccat cgtggtcttc atgaaccact accctctgga gaagatcggt  15240

gattccgtct gggacggaca tggcttctct gtgggtcaga tccatgagac catgaacatt  15300

cgacgaggca tcattactga ctggttcttt ggaggcctga actaccagat cgagcaccat  15360

ctctggccca ccctgcctcg acacaacctc actgccgttt cctaccaggt ggaacagctg  15420

tgccagaagc acaacctccc ctaccgaaac cctctgcccc atgaaggtct cgtcatcctg  15480

ctccgatacc tgtcccagtt cgctcgaatg gccgagaagc agcccggtgc caaggctcag  15540

taagcggccg catgagaaga taaatatata aatacattga gatattaaat gcgctagatt  15600

agagagcctc atactgctcg gagagaagcc aagacgagta ctcaaagggg attacaccat  15660

ccatatccac agacacaagc tggggaaagg ttctatatac actttccgga ataccgtagt  15720

ttccgatgtt atcaatgggg gcagccagga tttcaggcac ttcggtgtct cggggtgaaa  15780

tggcgttctt ggcctccatc aagtcgtacc atgtcttcat ttgcctgtca aagtaaaaca  15840

gaagcagatg aagaatgaac ttgaagtgaa ggaattt                           15877


<210>  133
<211>  4313
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZKUM

<400>  133
taatcgagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc     60

acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga    120

gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg    180

tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg    240

cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg    300

gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga    360

aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg    420

gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag    480

aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc    540

gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg    600

ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt    660

cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc    720

ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc    780

actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg    840

tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca    900

gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc    960

ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat   1020

cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt   1080

ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt   1140

tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc   1200

agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc   1260

gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata   1320

ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg   1380

gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc   1440

cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct   1500

acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa   1560

cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt   1620

cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca   1680

ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac   1740

tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca   1800

atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt   1860

tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc   1920

actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca   1980

aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata   2040

ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc   2100

ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc   2160

cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt   2220

acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc   2280

ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct   2340

ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat   2400

ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc   2460

acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc   2520

tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg   2580

atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttccattcgc   2640

cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc   2700

agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc   2760

agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat   2820

tgggtaccgg gccccccctc gaggtcgacg agtatctgtc tgactcgtca ttgccgcctt   2880

tggagtacga ctccaactat gagtgtgctt ggatcacttt gacgatacat tcttcgttgg   2940

aggctgtggg tctgacagct gcgttttcgg cgcggttggc cgacaacaat atcagctgca   3000

acgtcattgc tggctttcat catgatcaca tttttgtcgg caaaggcgac gcccagagag   3060

ccattgacgt tctttctaat ttggaccgat agccgtatag tccagtctat ctataagttc   3120

aactaactcg taactattac cataacatat acttcactgc cccagataag gttccgataa   3180

aaagttctgc agactaaatt tatttcagtc tcctcttcac caccaaaatg ccctcctacg   3240

aagctcgagt gctcaagctc gtggcagcca agaaaaccaa cctgtgtgct tctctggatg   3300

ttaccaccac caaggagctc attgagcttg ccgataaggt cggaccttat gtgtgcatga   3360

tcaaaaccca tatcgacatc attgacgact tcacctacgc cggcactgtg ctccccctca   3420

aggaacttgc tcttaagcac ggtttcttcc tgttcgagga cagaaagttc gcagatattg   3480

gcaacactgt caagcaccag taccggtgtc accgaatcgc cgagtggtcc gatatcacca   3540

acgcccacgg tgtacccgga accggaatcg attgctggcc tgcgagctgg tgcgtacgag   3600

gaaactgtct ctgaacagaa gaaggaggac gtctctgact acgagaactc ccagtacaag   3660

gagttcctag tcccctctcc caacgagaag ctggccagag gtctgctcat gctggccgag   3720

ctgtcttgca agggctctct ggccactggc gagtactcca agcagaccat tgagcttgcc   3780

cgatccgacc ccgagtttgt ggttggcttc attgcccaga accgacctaa gggcgactct   3840

gaggactggc ttattctgac ccccggggtg ggtcttgacg acaagggaga cgctctcgga   3900

cagcagtacc gaactgttga ggatgtcatg tctaccggaa cggatatcat aattgtcggc   3960

cgaggtctgt acggccagaa ccgagatcct attgaggagg ccaagcgata ccagaaggct   4020

ggctgggagg cttaccagaa gattaactgt tagaggttag actatggata tgtaatttaa   4080

ctgtgtatat agagagcgtg caagtatgga gcgcttgttc agcttgtatg atggtcagac   4140

gacctgtctg atcgagtatg tatgatactg cacaacctgt gtatccgcat gatctgtcca   4200

atggggcatg ttgttgtgtt tctcgatacg gagatgctgg gtacagtgct aatacgttga   4260

actacttata cttatatgag gctcgaagaa agctgacttg tgtatgactt aat          4313


<210>  134
<211>  1459
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic mutant Ura3 gene, comprising a 33 bp deletion from +21 
       to +53, a  1 bp deletion at +376 and a 3 bp deletion from +400 to
       403 of the Yarrowia Ura3 coding region (GenBank Accession No. 
       AJ306421)

<400>  134
gagtatctgt ctgactcgtc attgccgcct ttggagtacg actccaacta tgagtgtgct     60

tggatcactt tgacgataca ttcttcgttg gaggctgtgg gtctgacagc tgcgttttcg    120

gcgcggttgg ccgacaacaa tatcagctgc aacgtcattg ctggctttca tcatgatcac    180

atttttgtcg gcaaaggcga cgcccagaga gccattgacg ttctttctaa tttggaccga    240

tagccgtata gtccagtcta tctataagtt caactaactc gtaactatta ccataacata    300

tacttcactg ccccagataa ggttccgata aaaagttctg cagactaaat ttatttcagt    360

ctcctcttca ccaccaaaat gccctcctac gaagctcgag tgctcaagct cgtggcagcc    420

aagaaaacca acctgtgtgc ttctctggat gttaccacca ccaaggagct cattgagctt    480

gccgataagg tcggacctta tgtgtgcatg atcaaaaccc atatcgacat cattgacgac    540

ttcacctacg ccggcactgt gctccccctc aaggaacttg ctcttaagca cggtttcttc    600

ctgttcgagg acagaaagtt cgcagatatt ggcaacactg tcaagcacca gtaccggtgt    660

caccgaatcg ccgagtggtc cgatatcacc aacgcccacg gtgtacccgg aaccggaatc    720

gattgctggc ctgcgagctg gtgcgtacga ggaaactgtc tctgaacaga agaaggagga    780

cgtctctgac tacgagaact cccagtacaa ggagttccta gtcccctctc ccaacgagaa    840

gctggccaga ggtctgctca tgctggccga gctgtcttgc aagggctctc tggccactgg    900

cgagtactcc aagcagacca ttgagcttgc ccgatccgac cccgagtttg tggttggctt    960

cattgcccag aaccgaccta agggcgactc tgaggactgg cttattctga cccccggggt   1020

gggtcttgac gacaagggag acgctctcgg acagcagtac cgaactgttg aggatgtcat   1080

gtctaccgga acggatatca taattgtcgg ccgaggtctg tacggccaga accgagatcc   1140

tattgaggag gccaagcgat accagaaggc tggctgggag gcttaccaga agattaactg   1200

ttagaggtta gactatggat atgtaattta actgtgtata tagagagcgt gcaagtatgg   1260

agcgcttgtt cagcttgtat gatggtcaga cgacctgtct gatcgagtat gtatgatact   1320

gcacaacctg tgtatccgca tgatctgtcc aatggggcat gttgttgtgt ttctcgatac   1380

ggagatgctg ggtacagtgc taatacgttg aactacttat acttatatga ggctcgaaga   1440

aagctgactt gtgtatgac                                                1459


<210>  135
<211>  15966
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZKD2-5U89A2

<400>  135
gtacgtttca tgaaggcggg cagaaagtac tcgatggtgg agatgattgc tcggaggtac     60

ttgttctgcg gccagtatct ctcagcaatc aggtgatact cctggacgtc cagagggtag    120

tatgtgtgcg tgggctccag atccaccgtc ttgtgcagag ttatggggaa gtagcggcca    180

aagagcttcc agatgaagaa gtttcttgaa ataggcgagt atcgcttgac cactcctccg    240

ttggacgggg agtcgtcttt aacagcgtac actacatacg caatcacaaa tggccagagc    300

agtggaattg cgcagcatag catgaaaatt gtgaggaaag tgggaatgct gaaaatgtgc    360

cagaccagag agaaggtctc acatcggttg agtaatggtg tcgatagcgg ggcatatcgg    420

attcccgcga ttttgggtgc cgtgtcgttt ttgtctcgcg acttgtagta ttgtgagtcg    480

atagtcatag cttttgtttt gtgtgacttg tctgttgcct gttgttagaa gaaaaagtgg    540

gagcttatca gtcacggtcc acgaacgatt tcgtacttgt acgtaattgg tcgtgagaac    600

tgttgcagag ccggtgcttt tttttgtggc caagtcgaca ggtcgatttc ggcgctgtgc    660

gaggttgctg ggatgtgctg gtttggctgc caaatgtggg gaagatttca acctcggatt    720

tgacgtgtgt agaggcgcgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg    780

gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc    840

ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag    900

gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa    960

aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc   1020

gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc   1080

ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg   1140

cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt   1200

cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc   1260

gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc   1320

cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag   1380

agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg   1440

ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa   1500

ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag   1560

gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact   1620

cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa   1680

attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt   1740

accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag   1800

ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca   1860

gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc   1920

agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt   1980

ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg   2040

ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca   2100

gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg   2160

ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca   2220

tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg   2280

tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct   2340

cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca   2400

tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca   2460

gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg   2520

tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac   2580

ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt   2640

attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc   2700

cgcgcacatt tccccgaaaa gtgccacctg atgcggtgtg aaataccgca cagatgcgta   2760

aggagaaaat accgcatcag gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa   2820

atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata   2880

aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac   2940

tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc   3000

cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa   3060

atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg   3120

cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg   3180

tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcca   3240

ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt   3300

acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt   3360

ttcccagtca cgacgttgta aaacgacggc cagtgaattg taatacgact cactataggg   3420

cgaattgggc ccgacgtcgc atgcatcaaa ggaagggtga atccaaggaa gttcttgaca   3480

aactgctgga atcggtacag cttggacgac ttgtcgttgc taacctggtc atagaggtcg   3540

ttctcaccaa aggccatgat gggaacaagg gcgacatttc cgacctccat accaagtcga   3600

acaaaaccct ttcgcttgag tagcaccagg tccatgacac cgggtctggc cagaagactt   3660

tcctgtgctc caccaacgac aatgcagata gactggtttc gcttgaggag ggccttgcag   3720

gacttcttgg agacagaagc gactcccaga ctcatgaggt actctctgta gagaggcact   3780

cggaagttgt tggtgagagt cataagagaa acagggatgc ccggaaagag cttggaccat   3840

ccagctccct cggtggcaat tccaccaaag gctcccatgc cgataatgcc gtgggggtgg   3900

tagccgaaga tgtattttct gccagtgggc ttgagttttg tgggcgacag ctgtgggtcg   3960

ttttcgccaa tgatctggtt ggcgtaggag ttgagggacc cgttaagaag cgtggaatca   4020

gatgcagtgg agccagcaga ggcggacgac aaaggtcgtc ggttagtggt gccattgttg   4080

ccgttgccgt taagttcgga gcccgaggcg tggccgttgg agccagatga ttctccacgg   4140

ctatatctgc tgtcgtggtt aattaactca cctgcaggat tgagactatg aatggattcc   4200

cgtgcccgta ttactctact aatttgatct tggaacgcga aaatacgttt ctaggactcc   4260

aaagaatctc aactcttgtc cttactaaat atactaccca tagttgatgg tttacttgaa   4320

cagagaggac atgttcactt gacccaaagt ttctcgcatc tcttggatat ttgaacaacg   4380

gcgtccactg accgtcagtt atccagtcac aaaaccccca cattcataca ttcccatgta   4440

cgtttacaaa gttctcaatt ccatcgtgca aatcaaaatc acatctattc attcatcata   4500

tataaaccca tcatgtctac taacactcac aactccatag aaaacatcga ctcagaacac   4560

acgctccatg cggccgctta ggaatcctga gcgtccttga cacagtgaac cacaccgact   4620

ttgtgcatgt acttgagggt ggaaatgatg ttgcccacaa tggtagggta gaagacgtac   4680

cgaactccgt gtcgttcgca acactctcgg acagcttgct gcacgaaggg atagtgccaa   4740

gacgacattc gaggaaagag gtgatgctcg atctggaagt tgagaccgcc agtaaagaac   4800

atggcaatgg gtccaccgta ggtggaagag gtctccacct gagctctgta ccagtcgatc   4860

tgatcggctt caacgtcctt ctcggagctc ttgaccttgc agttcttgtc ggggattcgc   4920

tccgagccat cgaagttgtg agacaagatg aaaaagaagg tgaggaaggc accggtagca   4980

gtgggcacca gaggaatggt gatgagcagg gaggttccag tgagatacca gggcaagaag   5040

gcggttcgaa agatgaagaa agctcgcata acgaatgcaa gggttcggta ccgtcgcaga   5100

aagccgttct ctcgcatggc tgtgacagac tcgggaatgg tgtcgttgtg ctgcattcgg   5160

aagatgtaga gagggttgta caccagcgaa acgccgtagg ctccaagcac gaggtacatg   5220

taccaggcct ggaatcggtg aaaccacttt cgagcagtgt tggcagcagg gtagttgtgg   5280

aacacaagga atggttctgc ggactcggca tccaggtcga gaccatgctg attggtgtag   5340

gtgtgatgtc gcatgatgtg agactgcagc cagatccatc tggacgatcc aatgacgtcg   5400

atgccgtagg caaagagagc gttgacccag ggctttttgc tgatggcacc atgagaggca   5460

tcgtgctgaa tggacaggcc gatctgcatg tgcatgaatc cagtcaagag accccacagc   5520

accattccgg tagtagccca gtgccactcg caaaaggcgg tgacagcaat gatgccaacg   5580

gttcgcagcc agaatccagg tgtggcatac cagttccgac ctttcatgac ctctcgcata   5640

gttcgcttga cgtcctgtgc aaagggagag tcgtaggtgt agacaatgtc cttggaggtt   5700

cggtcgtgct tgcctcgcac gaactgttga agcagcttcg agttctcggg cttgacgtaa   5760

gggtgcatgg agtagaacag aggagaagca tcggaggcac cagaagcgag gatcaagtcg   5820

cctccgggat ggaccttggc aagaccttcc agatcgtaga gaatgccgtc gatggcaacc   5880

aggtcgggtc gctcgagcag ctgctcggta gtaagggaga gagccatggc cattgctgta   5940

gatatgtctt gtgtgtaagg gggttggggt ggttgtttgt gttcttgact tttgtgttag   6000

caagggaaga cgggcaaaaa agtgagtgtg gttgggaggg agagacgagc cttatatata   6060

atgcttgttt gtgtttgtgc aagtggacgc cgaaacgggc aggagccaaa ctaaacaagg   6120

cagacaatgc gagcttaatt ggattgcctg atgggcaggg gttagggctc gatcaatggg   6180

ggtgcgaagt gacaaaattg ggaattaggt tcgcaagcaa ggctgacaag actttggccc   6240

aaacatttgt acgcggtgga caacaggagc cacccatcgt ctgtcacggg ctagccggtc   6300

gtgcgtcctg tcaggctcca cctaggctcc atgccactcc atacaatccc actagtgtac   6360

cgctaggccg cttttagctc ccatctaaga cccccccaaa acctccactg tacagtgcac   6420

tgtactgtgt ggcgatcaag ggcaagggaa aaaaggcgca aacatgcacg catggaatga   6480

cgtaggtaag gcgttactag actgaaaagt ggcacatttc ggcgtgccaa agggtcctag   6540

gtgcgtttcg cgagctgggc gccaggccaa gccgctccaa aacgcctctc cgactccctc   6600

cagcggcctc catatcccca tccctctcca cagcaatgtt gttaagcctt gcaaacgaaa   6660

aaatagaaag gctaataagc ttccaatatt gtggtgtacg ctgcataacg caacaatgag   6720

cgccaaacaa cacacacaca cagcacacag cagcattaac cacgatgaac agcatgaatt   6780

ctctctcttg agcttttcca taacaagttc ttctgcctcc aggaagtcca tgggtggttt   6840

gatcatggtt ttggtgtagt ggtagtgcag tggtggtatt gtgactgggg atgtagttga   6900

gaataagtca tacacaagtc agctttcttc gagcctcata taagtataag tagttcaacg   6960

tattagcact gtacccagca tctccgtatc gagaaacaca acaacatgcc ccattggaca   7020

gatcatgcgg atacacaggt tgtgcagtat catacatact cgatcagaca ggtcgtctga   7080

ccatcataca agctgaacaa gcgctccata cttgcacgct ctctatatac acagttaaat   7140

tacatatcca tagtctaacc tctaacagtt aatcttctgg taagcctccc agccagcctt   7200

ctggtatcgc ttggcctcct caataggatc tcggttctgg ccgtacagac ctcggccgac   7260

aattatgata tccgttccgg tagacatgac atcctcaaca gttcggtact gctgtccgag   7320

agcgtctccc ttgtcgtcaa gacccacccc gggggtcaga ataagccagt cctcagagtc   7380

gcccttaggt cggttctggg caatgaagcc aaccacaaac tcggggtcgg atcgggcaag   7440

ctcaatggtc tgcttggagt actcgccagt ggccagagag cccttgcaag acagctcggc   7500

cagcatgagc agacctctgg ccagcttctc gttgggagag gggactagga actccttgta   7560

ctgggagttc tcgtagtcag agacgtcctc cttcttctgt tcagagacag tttcctcggc   7620

accagctcgc aggccagcaa tgattccggt tccgggtaca ccgtgggcgt tggtgatatc   7680

ggaccactcg gcgattcggt gacaccggta ctggtgcttg acagtgttgc caatatctgc   7740

gaactttctg tcctcgaaca ggaagaaacc gtgcttaaga gcaagttcct tgagggggag   7800

cacagtgccg gcgtaggtga agtcgtcaat gatgtcgata tgggttttga tcatgcacac   7860

ataaggtccg accttatcgg caagctcaat gagctccttg gtggtggtaa catccagaga   7920

agcacacagg ttggttttct tggctgccac gagcttgagc actcgagcgg caaaggcgga   7980

cttgtggacg ttagctcgag cttcgtagga gggcattttg gtggtgaaga ggagactgaa   8040

ataaatttag tctgcagaac tttttatcgg aaccttatct ggggcagtga agtatatgtt   8100

atggtaatag ttacgagtta gttgaactta tagatagact ggactatacg gctatcggtc   8160

caaattagaa agaacgtcaa tggctctctg ggcgtcgcct ttgccgacaa aaatgtgatc   8220

atgatgaaag ccagcaatga cgttgcagct gatattgttg tcggccaacc gcgccgaaaa   8280

cgcagctgtc agacccacag cctccaacga agaatgtatc gtcaaagtga tccaagcaca   8340

ctcatagttg gagtcgtact ccaaaggcgg caatgacgag tcagacagat actcgtcgac   8400

cttttccttg ggaaccacca ccgtcagccc ttctgactca cgtattgtag ccaccgacac   8460

aggcaacagt ccgtggatag cagaatatgt cttgtcggtc catttctcac caactttagg   8520

cgtcaagtga atgttgcaga agaagtatgt gccttcattg agaatcggtg ttgctgattt   8580

caataaagtc ttgagatcag tttggccagt catgttgtgg ggggtaattg gattgagtta   8640

tcgcctacag tctgtacagg tatactcgct gcccacttta tactttttga ttccgctgca   8700

cttgaagcaa tgtcgtttac caaaagtgag aatgctccac agaacacacc ccagggtatg   8760

gttgagcaaa aaataaacac tccgatacgg ggaatcgaac cccggtctcc acggttctca   8820

agaagtattc ttgatgagag cgtatcgata gttggagcaa gggagaaatg tagagtgtga   8880

aagactcact atggtccggg cttatctcga ccaatagcca aagtctggag tttctgagag   8940

aaaaaggcaa gatacgtatg taacaaagcg acgcatggta caataatacc ggaggcatgt   9000

atcatagaga gttagtggtt cgatgatggc actggtgcct ggtatgactt tatacggctg   9060

actacatatt tgtcctcaga catacaatta cagtcaagca cttacccttg gacatctgta   9120

ggtacccccc ggccaagacg atctcagcgt gtcgtatgtc ggattggcgt agctccctcg   9180

ctcgtcaatt ggctcccatc tactttcttc tgcttggcta cacccagcat gtctgctatg   9240

gctcgttttc gtgccttatc tatcctccca gtattaccaa ctctaaatga catgatgtga   9300

ttgggtctac actttcatat cagagataag gagtagcaca gttgcataaa aagcccaact   9360

ctaatcagct tcttcctttc ttgtaattag tacaaaggtg attagcgaaa tctggaagct   9420

tagttggccc taaaaaaatc aaaaaaagca aaaaacgaaa aacgaaaaac cacagttttg   9480

agaacaggga ggtaacgaag gatcgtatat atatatatat atatatatac ccacggatcc   9540

cgagaccggc ctttgattct tccctacaac caaccattct caccacccta attcacaacc   9600

atggctgccg tcatcgaggt ggccaacgag ttcgtcgcta tcactgccga gacccttccc   9660

aaggtggact atcagcgact ctggcgagac atctactcct gcgagctcct gtacttctcc   9720

attgctttcg tcatcctcaa gtttaccctt ggcgagctct cggattctgg caaaaagatt   9780

ctgcgagtgc tgttcaagtg gtacaacctc ttcatgtccg tcttttcgct ggtgtccttc   9840

ctctgtatgg gttacgccat ctacaccgtt ggactgtact ccaacgaatg cgacagagct   9900

ttcgacaaca gcttgttccg atttgccacc aaggtcttct actattccaa gtttctggag   9960

tacatcgact ctttctacct tcccctcatg gccaagcctc tgtcctttct gcagttcttt  10020

catcacttgg gagctcctat ggacatgtgg ctcttcgtgc agtactctgg cgaatccatt  10080

tggatctttg tgttcctgaa cggattcatt cactttgtca tgtacggcta ctattggaca  10140

cggctgatga agttcaactt tcccatgccc aagcagctca ttaccgcaat gcagatcacc  10200

cagttcaacg ttggcttcta cctcgtgtgg tggtacaagg acattccctg ttaccgaaag  10260

gatcccatgc gaatgctggc ctggatcttc aactactggt acgtcggtac cgttcttctg  10320

ctcttcatca acttctttgt caagtcctac gtgtttccca agcctaagac tgccgacaaa  10380

aaggtccagt agcggccgca tgtacataca agattattta tagaaatgaa tcgcgatcga  10440

acaaagagta cgagtgtacg agtaggggat gatgataaaa gtggaagaag ttccgcatct  10500

ttggatttat caacgtgtag gacgatactt cctgtaaaaa tgcaatgtct ttaccatagg  10560

ttctgctgta gatgttatta actaccatta acatgtctac ttgtacagtt gcagaccagt  10620

tggagtatag aatggtacac ttaccaaaaa gtgttgatgg ttgtaactac gatatataaa  10680

actgttgacg ggatctgtat attcggtaag atatattttg tggggtttta gtggtgttta  10740

aacaccacta aaaccccaca aaatatatct taccgaatat acagatctac tatagaggaa  10800

caattgcccc ggagaagacg gccaggccgc ctagatgaca aattcaacaa ctcacagctg  10860

actttctgcc attgccacta ggggggggcc tttttatatg gccaagccaa gctctccacg  10920

tcggttgggc tgcacccaac aataaatggg tagggttgca ccaacaaagg gatgggatgg  10980

ggggtagaag atacgaggat aacggggctc aatggcacaa ataagaacga atactgccat  11040

taagactcgt gatccagcga ctgacaccat tgcatcatct aagggcctca aaactacctc  11100

ggaactgctg cgctgatctg gacaccacag aggttccgag cactttaggt tgcaccaaat  11160

gtcccaccag gtgcaggcag aaaacgctgg aacagcgtgt acagtttgtc ttaacaaaaa  11220

gtgagggcgc tgaggtcgag cagggtggtg tgacttgtta tagcctttag agctgcgaaa  11280

gcgcgtatgg atttggctca tcaggccaga ttgagggtct gtggacacat gtcatgttag  11340

tgtacttcaa tcgccccctg gatatagccc cgacaatagg ccgtggcctc atttttttgc  11400

cttccgcaca tttccattgc tcggtaccca caccttgctt ctcctgcact tgccaacctt  11460

aatactggtt tacattgacc aacatcttac aagcgggggg cttgtctagg gtatatataa  11520

acagtggctc tcccaatcgg ttgccagtct cttttttcct ttctttcccc acagattcga  11580

aatctaaact acacatcaca caatgcctgt tactgacgtc cttaagcgaa agtccggtgt  11640

catcgtcggc gacgatgtcc gagccgtgag tatccacgac aagatcagtg tcgagacgac  11700

gcgttttgtg taatgacaca atccgaaagt cgctagcaac acacactctc tacacaaact  11760

aacccagctc tccatggtga aggcttctcg acaggctctg cccctcgtca tcgacggaaa  11820

ggtgtacgac gtctccgctt gggtgaactt ccaccctggt ggagctgaaa tcattgagaa  11880

ctaccaggga cgagatgcta ctgacgcctt catggttatg cactctcagg aagccttcga  11940

caagctcaag cgaatgccca agatcaacca ggcttccgag ctgcctcccc aggctgccgt  12000

caacgaagct caggaggatt tccgaaagct ccgagaagag ctgatcgcca ctggcatgtt  12060

tgacgcctct cccctctggt actcgtacaa gatcttgacc accctgggtc ttggcgtgct  12120

tgccttcttc atgctggtcc agtaccacct gtacttcatt ggtgctctcg tgctcggtat  12180

gcactaccag caaatgggat ggctgtctca tgacatctgc caccaccaga ccttcaagaa  12240

ccgaaactgg aataacgtcc tgggtctggt ctttggcaac ggactccagg gcttctccgt  12300

gacctggtgg aaggacagac acaacgccca tcattctgct accaacgttc agggtcacga  12360

tcccgacatt gataacctgc ctctgctcgc ctggtccgag gacgatgtca ctcgagcttc  12420

tcccatctcc cgaaagctca ttcagttcca acagtactat ttcctggtca tctgtattct  12480

cctgcgattc atctggtgtt tccagtctgt gctgaccgtt cgatccctca aggaccgaga  12540

caaccagttc taccgatctc agtacaagaa agaggccatt ggactcgctc tgcactggac  12600

tctcaagacc ctgttccacc tcttctttat gccctccatc ctgacctcga tgctggtgtt  12660

ctttgtttcc gagctcgtcg gtggcttcgg aattgccatc gtggtcttca tgaaccacta  12720

ccctctggag aagatcggtg attccgtctg ggacggacat ggcttctctg tgggtcagat  12780

ccatgagacc atgaacattc gacgaggcat cattactgac tggttctttg gaggcctgaa  12840

ctaccagatc gagcaccatc tctggcccac cctgcctcga cacaacctca ctgccgtttc  12900

ctaccaggtg gaacagctgt gccagaagca caacctcccc taccgaaacc ctctgcccca  12960

tgaaggtctc gtcatcctgc tccgatacct gtcccagttc gctcgaatgg ccgagaagca  13020

gcccggtgcc aaggctcagt aagcggccgc atgagaagat aaatatataa atacattgag  13080

atattaaatg cgctagatta gagagcctca tactgctcgg agagaagcca agacgagtac  13140

tcaaagggga ttacaccatc catatccaca gacacaagct ggggaaaggt tctatataca  13200

ctttccggaa taccgtagtt tccgatgtta tcaatggggg cagccaggat ttcaggcact  13260

tcggtgtctc ggggtgaaat ggcgttcttg gcctccatca agtcgtacca tgtcttcatt  13320

tgcctgtcaa agtaaaacag aagcagatga agaatgaact tgaagtgaag gaatttaaat  13380

agttggagca agggagaaat gtagagtgtg aaagactcac tatggtccgg gcttatctcg  13440

accaatagcc aaagtctgga gtttctgaga gaaaaaggca agatacgtat gtaacaaagc  13500

gacgcatggt acaataatac cggaggcatg tatcatagag agttagtggt tcgatgatgg  13560

cactggtgcc tggtatgact ttatacggct gactacatat ttgtcctcag acatacaatt  13620

acagtcaagc acttaccctt ggacatctgt aggtaccccc cggccaagac gatctcagcg  13680

tgtcgtatgt cggattggcg tagctccctc gctcgtcaat tggctcccat ctactttctt  13740

ctgcttggct acacccagca tgtctgctat ggctcgtttt cgtgccttat ctatcctccc  13800

agtattacca actctaaatg acatgatgtg attgggtcta cactttcata tcagagataa  13860

ggagtagcac agttgcataa aaagcccaac tctaatcagc ttcttccttt cttgtaatta  13920

gtacaaaggt gattagcgaa atctggaagc ttagttggcc ctaaaaaaat caaaaaaagc  13980

aaaaaacgaa aaacgaaaaa ccacagtttt gagaacaggg aggtaacgaa ggatcgtata  14040

tatatatata tatatatata cccacggatc ccgagaccgg cctttgattc ttccctacaa  14100

ccaaccattc tcaccaccct aattcacaac catggcctcc acctcggctc tgcccaagca  14160

gaaccctgcc ctccgacgaa ccgtcacttc caccactgtg accgactcgg agtctgctgc  14220

cgtctctccc tccgattctc ccagacactc ggcctcctct acatcgctgt cttccatgtc  14280

cgaggtggac attgccaagc ccaagtccga gtacggtgtc atgctggata cctacggcaa  14340

ccagttcgaa gttcccgact tcaccatcaa ggacatctac aacgctattc ccaagcactg  14400

cttcaagcga tctgctctca agggatacgg ctacattctt cgagacattg tcctcctgac  14460

taccactttc agcatctggt acaactttgt gacacccgag tacattccct ccactcctgc  14520

tcgagccggt ctgtgggctg tgtacaccgt tcttcaggga ctcttcggta ctggactgtg  14580

ggtcattgcc cacgagtgtg gacatggtgc tttctccgat tcccgaatca tcaacgacat  14640

tactggctgg gtgcttcact cttccctgct tgttccctac ttcagctggc aaatctccca  14700

ccggaagcat cacaaggcca ctggaaacat ggagcgagac atggtcttcg ttcctcgaac  14760

ccgagagcag caagctactc gactcggcaa gatgacccac gaactcgccc atcttaccga  14820

ggaaactcct gctttcaccc tgctcatgct tgtgcttcag caactggtcg gttggcccaa  14880

ctatctcatt accaacgtta ctggacacaa ctaccatgag cggcagcgag agggtcgagg  14940

caagggaaag cacaacggtc ttggcggtgg agttaaccat ttcgatcccc gatctcctct  15000

gtacgagaac agcgacgcca agctcatcgt gctctccgac attggcattg gtcttatggc  15060

caccgctctg tactttctcg ttcagaagtt cggattctac aacatggcca tctggtactt  15120

cgttccctac ttgtgggtta accactggct cgtcgccatt acctttctgc agcacacaga  15180

tcctactctt ccccactaca ccaacgacga gtggaacttt gtgcgaggtg ccgctgcaac  15240

catcgaccga gagatgggct tcattggacg tcatctgctc cacggcatta tcgagactca  15300

cgtcctgcat cactacgtct cttccattcc cttctacaat gcggacgaag ctaccgaggc  15360

catcaaacct atcatgggca agcactatcg agctgatgtc caggacggtc ctcgaggatt  15420

cattcgagcc atgtaccgat ctgcacgaat gtgccagtgg gttgaaccct ccgctggtgc  15480

cgagggagct ggcaagggtg tcctgttctt tcgaaaccga aacaatgtgg gcactcctcc  15540

cgctgtcatc aagcccgttg cctaagcggc cgctatttat cactctttac aacttctacc  15600

tcaactatct actttaataa atgaatatcg tttattctct atgattactg tatatgcgtt  15660

cctctaagac aaatcgaaac cagcatgtga tcgaatggca tacaaaagtt tcttccgaag  15720

ttgatcaatg tcctgatagt caggcagctt gagaagattg acacaggtgg aggccgtagg  15780

gaaccgatca acctgtctac cagcgttacg aatggcaaat gacgggttca aagccttgaa  15840

tccttgcaat ggtgccttgg atactgatgt cacaaactta agaagcagcc gcttgtcctc  15900

ttcctcgatc gatggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca  15960

cacaac                                                             15966


<210>  136
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer pZP-GW-5-1

<400>  136
cgacaagatg gaatgagaat g                                               21


<210>  137
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer pZP-GW-5-2

<400>  137
ctggtttttc aactacttct ac                                              22


<210>  138
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer pZP-GW-5-3

<400>  138
gtactgtcct gtgtctgttc c                                               21


<210>  139
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer pZP-GW-5-4

<400>  139
ctacatcgtc cgaaagcaca ag                                              22


<210>  140
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer pZP-GW-3-1

<400>  140
ctaccagatc gagcaccatc tctg                                            24


<210>  141
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer pZP-GW-3-2

<400>  141
ctaccaggtg gaacagctgt g                                               21


<210>  142
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer pZP-GW-3-3

<400>  142
tctgccccat gaaggtctcg tc                                              22


<210>  143
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer pZP-GW-3-4

<400>  143
cctgtcccag ttcgctcgaa tg                                              22


<210>  144
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Genome Walker adaptor-1

<400>  144
gtaatacgac tatagggcac gcgtggtcga cggcccgggc tggt                      44


<210>  145
<211>  8
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Genome Walker adaptor-2


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5' end is associated with a -PO4 group

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  3' end is associated with a -H2N group

<400>  145
accagccc                                                               8


<210>  146
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nested adaptor primer

<400>  146
gtaatacgac tcactatagg gc                                              22


<210>  147
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Per10F1

<400>  147
gatcaaccat ggggggaagt tcacatgcat tcgctg                               36


<210>  148
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer ZPGW-5-5

<400>  148
gttatagttt tcatgtgaaa taccgagag                                       29


<210>  149
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Per10R

<400>  149
gatcaagcgg ccgccagacc tcgtcattat ctgatag                              37


<210>  150
<211>  7222
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pFBAIn-MOD-1

<400>  150
catggatcca ggcctgttaa cggccattac ggcctgcagg atccgaaaaa acctcccaca     60

cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc    120

agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt    180

ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgcgg    240

ccgcaagtgt ggatggggaa gtgagtgccc ggttctgtgt gcacaattgg caatccaaga    300

tggatggatt caacacaggg atatagcgag ctacgtggtg gtgcgaggat atagcaacgg    360

atatttatgt ttgacacttg agaatgtacg atacaagcac tgtccaagta caatactaaa    420

catactgtac atactcatac tcgtacccgg gcaacggttt cacttgagtg cagtggctag    480

tgctcttact cgtacagtgt gcaatactgc gtatcatagt ctttgatgta tatcgtattc    540

attcatgtta gttgcgtacg agccggaagc ataaagtgta aagcctgggg tgcctaatga    600

gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg    660

tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg    720

cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg    780

gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga    840

aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg    900

gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag    960

aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc   1020

gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg   1080

ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt   1140

cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc   1200

ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc   1260

actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg   1320

tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca   1380

gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc   1440

ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat   1500

cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt   1560

ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt   1620

tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc   1680

agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc   1740

gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata   1800

ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg   1860

gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc   1920

cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct   1980

acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa   2040

cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt   2100

cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca   2160

ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac   2220

tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca   2280

atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt   2340

tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc   2400

actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca   2460

aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata   2520

ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc   2580

ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc   2640

cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt   2700

acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc   2760

ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct   2820

ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat   2880

ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc   2940

acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc   3000

tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg   3060

atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttccattcgc   3120

cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc   3180

agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc   3240

agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat   3300

tgggtaccgg gccccccctc gaggtcgatg gtgtcgataa gcttgatatc gaattcatgt   3360

cacacaaacc gatcttcgcc tcaaggaaac ctaattctac atccgagaga ctgccgagat   3420

ccagtctaca ctgattaatt ttcgggccaa taatttaaaa aaatcgtgtt atataatatt   3480

atatgtatta tatatataca tcatgatgat actgacagtc atgtcccatt gctaaataga   3540

cagactccat ctgccgcctc caactgatgt tctcaatatt taaggggtca tctcgcattg   3600

tttaataata aacagactcc atctaccgcc tccaaatgat gttctcaaaa tatattgtat   3660

gaacttattt ttattactta gtattattag acaacttact tgctttatga aaaacacttc   3720

ctatttagga aacaatttat aatggcagtt cgttcattta acaatttatg tagaataaat   3780

gttataaatg cgtatgggaa atcttaaata tggatagcat aaatgatatc tgcattgcct   3840

aattcgaaat caacagcaac gaaaaaaatc ccttgtacaa cataaatagt catcgagaaa   3900

tatcaactat caaagaacag ctattcacac gttactattg agattattat tggacgagaa   3960

tcacacactc aactgtcttt ctctcttcta gaaatacagg tacaagtatg tactattctc   4020

attgttcata cttctagtca tttcatccca catattcctt ggatttctct ccaatgaatg   4080

acattctatc ttgcaaattc aacaattata ataagatata ccaaagtagc ggtatagtgg   4140

caatcaaaaa gcttctctgg tgtgcttctc gtatttattt ttattctaat gatccattaa   4200

aggtatatat ttatttcttg ttatataatc cttttgttta ttacatgggc tggatacata   4260

aaggtatttt gatttaattt tttgcttaaa ttcaatcccc cctcgttcag tgtcaactgt   4320

aatggtagga aattaccata cttttgaaga agcaaaaaaa atgaaagaaa aaaaaaatcg   4380

tatttccagg ttagacgttc cgcagaatct agaatgcggt atgcggtaca ttgttcttcg   4440

aacgtaaaag ttgcgctccc tgagatattg tacatttttg cttttacaag tacaagtaca   4500

tcgtacaact atgtactact gttgatgcat ccacaacagt ttgttttgtt tttttttgtt   4560

tttttttttt ctaatgattc attaccgcta tgtataccta cttgtacttg tagtaagccg   4620

ggttattggc gttcaattaa tcatagactt atgaatctgc acggtgtgcg ctgcgagtta   4680

cttttagctt atgcatgcta cttgggtgta atattgggat ctgttcggaa atcaacggat   4740

gctcaatcga tttcgacagt aattaattaa gtcatacaca agtcagcttt cttcgagcct   4800

catataagta taagtagttc aacgtattag cactgtaccc agcatctccg tatcgagaaa   4860

cacaacaaca tgccccattg gacagatcat gcggatacac aggttgtgca gtatcataca   4920

tactcgatca gacaggtcgt ctgaccatca tacaagctga acaagcgctc catacttgca   4980

cgctctctat atacacagtt aaattacata tccatagtct aacctctaac agttaatctt   5040

ctggtaagcc tcccagccag ccttctggta tcgcttggcc tcctcaatag gatctcggtt   5100

ctggccgtac agacctcggc cgacaattat gatatccgtt ccggtagaca tgacatcctc   5160

aacagttcgg tactgctgtc cgagagcgtc tcccttgtcg tcaagaccca ccccgggggt   5220

cagaataagc cagtcctcag agtcgccctt aggtcggttc tgggcaatga agccaaccac   5280

aaactcgggg tcggatcggg caagctcaat ggtctgcttg gagtactcgc cagtggccag   5340

agagcccttg caagacagct cggccagcat gagcagacct ctggccagct tctcgttggg   5400

agaggggact aggaactcct tgtactggga gttctcgtag tcagagacgt cctccttctt   5460

ctgttcagag acagtttcct cggcaccagc tcgcaggcca gcaatgattc cggttccggg   5520

tacaccgtgg gcgttggtga tatcggacca ctcggcgatt cggtgacacc ggtactggtg   5580

cttgacagtg ttgccaatat ctgcgaactt tctgtcctcg aacaggaaga aaccgtgctt   5640

aagagcaagt tccttgaggg ggagcacagt gccggcgtag gtgaagtcgt caatgatgtc   5700

gatatgggtt ttgatcatgc acacataagg tccgacctta tcggcaagct caatgagctc   5760

cttggtggtg gtaacatcca gagaagcaca caggttggtt ttcttggctg ccacgagctt   5820

gagcactcga gcggcaaagg cggacttgtg gacgttagct cgagcttcgt aggagggcat   5880

tttggtggtg aagaggagac tgaaataaat ttagtctgca gaacttttta tcggaacctt   5940

atctggggca gtgaagtata tgttatggta atagttacga gttagttgaa cttatagata   6000

gactggacta tacggctatc ggtccaaatt agaaagaacg tcaatggctc tctgggcgtc   6060

gcctttgccg acaaaaatgt gatcatgatg aaagccagca atgacgttgc agctgatatt   6120

gttgtcggcc aaccgcgccg aaaacgcagc tgtcagaccc acagcctcca acgaagaatg   6180

tatcgtcaaa gtgatccaag cacactcata gttggagtcg tactccaaag gcggcaatga   6240

cgagtcagac agatactcgt cgaaaacagt gtacgcagat ctactataga ggaacattta   6300

aattgccccg gagaagacgg ccaggccgcc tagatgacaa attcaacaac tcacagctga   6360

ctttctgcca ttgccactag gggggggcct ttttatatgg ccaagccaag ctctccacgt   6420

cggttgggct gcacccaaca ataaatgggt agggttgcac caacaaaggg atgggatggg   6480

gggtagaaga tacgaggata acggggctca atggcacaaa taagaacgaa tactgccatt   6540

aagactcgtg atccagcgac tgacaccatt gcatcatcta agggcctcaa aactacctcg   6600

gaactgctgc gctgatctgg acaccacaga ggttccgagc actttaggtt gcaccaaatg   6660

tcccaccagg tgcaggcaga aaacgctgga acagcgtgta cagtttgtct taacaaaaag   6720

tgagggcgct gaggtcgagc agggtggtgt gacttgttat agcctttaga gctgcgaaag   6780

cgcgtatgga tttggctcat caggccagat tgagggtctg tggacacatg tcatgttagt   6840

gtacttcaat cgccccctgg atatagcccc gacaataggc cgtggcctca tttttttgcc   6900

ttccgcacat ttccattgct cggtacccac accttgcttc tcctgcactt gccaacctta   6960

atactggttt acattgacca acatcttaca agcggggggc ttgtctaggg tatatataaa   7020

cagtggctct cccaatcggt tgccagtctc ttttttcctt tctttcccca cagattcgaa   7080

atctaaacta cacatcacag aattccgagc cgtgagtatc cacgacaaga tcagtgtcga   7140

gacgacgcgt tttgtgtaat gacacaatcc gaaagtcgct agcaacacac actctctaca   7200

caaactaacc cagctctggt ac                                            7222


<210>  151
<211>  8133
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pFBAIN-Pex10

<400>  151
ggccgcaagt gtggatgggg aagtgagtgc ccggttctgt gtgcacaatt ggcaatccaa     60

gatggatgga ttcaacacag ggatatagcg agctacgtgg tggtgcgagg atatagcaac    120

ggatatttat gtttgacact tgagaatgta cgatacaagc actgtccaag tacaatacta    180

aacatactgt acatactcat actcgtaccc gggcaacggt ttcacttgag tgcagtggct    240

agtgctctta ctcgtacagt gtgcaatact gcgtatcata gtctttgatg tatatcgtat    300

tcattcatgt tagttgcgta cgagccggaa gcataaagtg taaagcctgg ggtgcctaat    360

gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc    420

tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg    480

ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag    540

cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag    600

gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc    660

tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc    720

agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc    780

tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt    840

cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg    900

ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat    960

ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag   1020

ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt   1080

ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc   1140

cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta   1200

gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag   1260

atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga   1320

ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa   1380

gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa   1440

tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc   1500

ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga   1560

taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa   1620

gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt   1680

gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg   1740

ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc   1800

aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg   1860

gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag   1920

cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt   1980

actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt   2040

caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac   2100

gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac   2160

ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag   2220

caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa   2280

tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga   2340

gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc   2400

cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg   2460

ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct   2520

tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc   2580

ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg   2640

atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt   2700

ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg   2760

tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc   2820

tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttccattc   2880

gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg   2940

ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc   3000

ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga   3060

attgggtacc gggccccccc tcgaggtcga tggtgtcgat aagcttgata tcgaattcat   3120

gtcacacaaa ccgatcttcg cctcaaggaa acctaattct acatccgaga gactgccgag   3180

atccagtcta cactgattaa ttttcgggcc aataatttaa aaaaatcgtg ttatataata   3240

ttatatgtat tatatatata catcatgatg atactgacag tcatgtccca ttgctaaata   3300

gacagactcc atctgccgcc tccaactgat gttctcaata tttaaggggt catctcgcat   3360

tgtttaataa taaacagact ccatctaccg cctccaaatg atgttctcaa aatatattgt   3420

atgaacttat ttttattact tagtattatt agacaactta cttgctttat gaaaaacact   3480

tcctatttag gaaacaattt ataatggcag ttcgttcatt taacaattta tgtagaataa   3540

atgttataaa tgcgtatggg aaatcttaaa tatggatagc ataaatgata tctgcattgc   3600

ctaattcgaa atcaacagca acgaaaaaaa tcccttgtac aacataaata gtcatcgaga   3660

aatatcaact atcaaagaac agctattcac acgttactat tgagattatt attggacgag   3720

aatcacacac tcaactgtct ttctctcttc tagaaataca ggtacaagta tgtactattc   3780

tcattgttca tacttctagt catttcatcc cacatattcc ttggatttct ctccaatgaa   3840

tgacattcta tcttgcaaat tcaacaatta taataagata taccaaagta gcggtatagt   3900

ggcaatcaaa aagcttctct ggtgtgcttc tcgtatttat ttttattcta atgatccatt   3960

aaaggtatat atttatttct tgttatataa tccttttgtt tattacatgg gctggataca   4020

taaaggtatt ttgatttaat tttttgctta aattcaatcc cccctcgttc agtgtcaact   4080

gtaatggtag gaaattacca tacttttgaa gaagcaaaaa aaatgaaaga aaaaaaaaat   4140

cgtatttcca ggttagacgt tccgcagaat ctagaatgcg gtatgcggta cattgttctt   4200

cgaacgtaaa agttgcgctc cctgagatat tgtacatttt tgcttttaca agtacaagta   4260

catcgtacaa ctatgtacta ctgttgatgc atccacaaca gtttgttttg tttttttttg   4320

tttttttttt ttctaatgat tcattaccgc tatgtatacc tacttgtact tgtagtaagc   4380

cgggttattg gcgttcaatt aatcatagac ttatgaatct gcacggtgtg cgctgcgagt   4440

tacttttagc ttatgcatgc tacttgggtg taatattggg atctgttcgg aaatcaacgg   4500

atgctcaatc gatttcgaca gtaattaatt aagtcataca caagtcagct ttcttcgagc   4560

ctcatataag tataagtagt tcaacgtatt agcactgtac ccagcatctc cgtatcgaga   4620

aacacaacaa catgccccat tggacagatc atgcggatac acaggttgtg cagtatcata   4680

catactcgat cagacaggtc gtctgaccat catacaagct gaacaagcgc tccatacttg   4740

cacgctctct atatacacag ttaaattaca tatccatagt ctaacctcta acagttaatc   4800

ttctggtaag cctcccagcc agccttctgg tatcgcttgg cctcctcaat aggatctcgg   4860

ttctggccgt acagacctcg gccgacaatt atgatatccg ttccggtaga catgacatcc   4920

tcaacagttc ggtactgctg tccgagagcg tctcccttgt cgtcaagacc caccccgggg   4980

gtcagaataa gccagtcctc agagtcgccc ttaggtcggt tctgggcaat gaagccaacc   5040

acaaactcgg ggtcggatcg ggcaagctca atggtctgct tggagtactc gccagtggcc   5100

agagagccct tgcaagacag ctcggccagc atgagcagac ctctggccag cttctcgttg   5160

ggagagggga ctaggaactc cttgtactgg gagttctcgt agtcagagac gtcctccttc   5220

ttctgttcag agacagtttc ctcggcacca gctcgcaggc cagcaatgat tccggttccg   5280

ggtacaccgt gggcgttggt gatatcggac cactcggcga ttcggtgaca ccggtactgg   5340

tgcttgacag tgttgccaat atctgcgaac tttctgtcct cgaacaggaa gaaaccgtgc   5400

ttaagagcaa gttccttgag ggggagcaca gtgccggcgt aggtgaagtc gtcaatgatg   5460

tcgatatggg ttttgatcat gcacacataa ggtccgacct tatcggcaag ctcaatgagc   5520

tccttggtgg tggtaacatc cagagaagca cacaggttgg ttttcttggc tgccacgagc   5580

ttgagcactc gagcggcaaa ggcggacttg tggacgttag ctcgagcttc gtaggagggc   5640

attttggtgg tgaagaggag actgaaataa atttagtctg cagaactttt tatcggaacc   5700

ttatctgggg cagtgaagta tatgttatgg taatagttac gagttagttg aacttataga   5760

tagactggac tatacggcta tcggtccaaa ttagaaagaa cgtcaatggc tctctgggcg   5820

tcgcctttgc cgacaaaaat gtgatcatga tgaaagccag caatgacgtt gcagctgata   5880

ttgttgtcgg ccaaccgcgc cgaaaacgca gctgtcagac ccacagcctc caacgaagaa   5940

tgtatcgtca aagtgatcca agcacactca tagttggagt cgtactccaa aggcggcaat   6000

gacgagtcag acagatactc gtcgaaaaca gtgtacgcag atctactata gaggaacatt   6060

taaattgccc cggagaagac ggccaggccg cctagatgac aaattcaaca actcacagct   6120

gactttctgc cattgccact aggggggggc ctttttatat ggccaagcca agctctccac   6180

gtcggttggg ctgcacccaa caataaatgg gtagggttgc accaacaaag ggatgggatg   6240

gggggtagaa gatacgagga taacggggct caatggcaca aataagaacg aatactgcca   6300

ttaagactcg tgatccagcg actgacacca ttgcatcatc taagggcctc aaaactacct   6360

cggaactgct gcgctgatct ggacaccaca gaggttccga gcactttagg ttgcaccaaa   6420

tgtcccacca ggtgcaggca gaaaacgctg gaacagcgtg tacagtttgt cttaacaaaa   6480

agtgagggcg ctgaggtcga gcagggtggt gtgacttgtt atagccttta gagctgcgaa   6540

agcgcgtatg gatttggctc atcaggccag attgagggtc tgtggacaca tgtcatgtta   6600

gtgtacttca atcgccccct ggatatagcc ccgacaatag gccgtggcct catttttttg   6660

ccttccgcac atttccattg ctcggtaccc acaccttgct tctcctgcac ttgccaacct   6720

taatactggt ttacattgac caacatctta caagcggggg gcttgtctag ggtatatata   6780

aacagtggct ctcccaatcg gttgccagtc tcttttttcc tttctttccc cacagattcg   6840

aaatctaaac tacacatcac agaattccga gccgtgagta tccacgacaa gatcagtgtc   6900

gagacgacgc gttttgtgta atgacacaat ccgaaagtcg ctagcaacac acactctcta   6960

cacaaactaa cccagctctg gtaccatggg gggaagttca catgcattcg ctggtgaatc   7020

tgatctgaca ctacaactac acaccaggtc caacatgagc gacaatacga caatcaaaaa   7080

gccgatccga cccaaaccga tccggacgga acgcctgcct tacgctgggg ccgcagaaat   7140

catccgagcc aaccagaaag accactactt tgagtccgtg cttgaacagc atctcgtcac   7200

gtttctgcag aaatggaagg gagtacgatt tatccaccag tacaaggagg agctggagac   7260

ggcgtccaag tttgcatatc tcggtttgtg tacgcttgtg ggctccaaga ctctcggaga   7320

agagtacacc aatctcatgt acactatcag agaccgaaca gctctaccgg gggtggtgag   7380

acggtttggc tacgtgcttt ccaacactct gtttccatac ctgtttgtgc gctacatggg   7440

caagttgcgc gccaaactga tgcgcgagta tccccatctg gtggagtacg acgaagatga   7500

gcctgtgccc agcccggaaa catggaagga gcgggtcatc aagacgtttg tgaacaagtt   7560

tgacaagttc acggcgctgg aggggtttac cgcgatccac ttggcgattt tctacgtcta   7620

cggctcgtac taccagctca gtaagcggat ctggggcatg cgttatgtat ttggacaccg   7680

actggacaag aatgagcctc gaatcggtta cgagatgctc ggtctgctga ttttcgcccg   7740

gtttgccacg tcatttgtgc agacgggaag agagtacctc ggagcgctgc tggaaaagag   7800

cgtggagaaa gaggcagggg agaaggaaga tgaaaaggaa gcggttgtgc cgaaaaagaa   7860

gtcgtcaatt ccgttcattg aggatacaga aggggagacg gaagacaaga tcgatctgga   7920

ggaccctcga cagctcaagt tcattcctga ggcgtccaga gcgtgcactc tgtgtctgtc   7980

atacattagt gcgccggcat gtacgccatg tggacacttt ttctgttggg actgtatttc   8040

cgaatgggtg agagagaagc ccgagtgtcc cttgtgtcgg cagggtgtga gagagcagaa   8100

cttgttgcct atcagataat gacgaggtct ggc                                8133


<210>  152
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer PEX10-R-BsiWI

<400>  152
gatcaacgta cgcttcagca gtaactgtat tgctc                                35


<210>  153
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer PEX10-F1-SalI

<400>  153
gatcaagtcg acattgtaac tagtcctgga gggtc                                35


<210>  154
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer PEX10-F2-SalI

<400>  154
gatcaagtcg acgtcttagc gtcatgtatt ctcaag                               36


<210>  155
<211>  7277
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pEXP-MOD1

<400>  155
catggatcca ggcctgttaa cggccattac ggcctgcagg atccgaaaaa acctcccaca     60

cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc    120

agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt    180

ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgcgg    240

ccgcaagtgt ggatggggaa gtgagtgccc ggttctgtgt gcacaattgg caatccaaga    300

tggatggatt caacacaggg atatagcgag ctacgtggtg gtgcgaggat atagcaacgg    360

atatttatgt ttgacacttg agaatgtacg atacaagcac tgtccaagta caatactaaa    420

catactgtac atactcatac tcgtacccgg gcaacggttt cacttgagtg cagtggctag    480

tgctcttact cgtacagtgt gcaatactgc gtatcatagt ctttgatgta tatcgtattc    540

attcatgtta gttgcgtacg agccggaagc ataaagtgta aagcctgggg tgcctaatga    600

gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg    660

tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg    720

cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg    780

gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga    840

aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg    900

gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag    960

aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc   1020

gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg   1080

ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt   1140

cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc   1200

ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc   1260

actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg   1320

tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca   1380

gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc   1440

ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat   1500

cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt   1560

ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt   1620

tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc   1680

agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc   1740

gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata   1800

ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg   1860

gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc   1920

cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct   1980

acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa   2040

cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt   2100

cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca   2160

ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac   2220

tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca   2280

atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt   2340

tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc   2400

actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca   2460

aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata   2520

ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc   2580

ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc   2640

cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt   2700

acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc   2760

ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct   2820

ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat   2880

ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc   2940

acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc   3000

tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg   3060

atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttccattcgc   3120

cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc   3180

agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc   3240

agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat   3300

tgggtaccgg gccccccctc gaggtcgatg gtgtcgataa gcttgatatc gaattcatgt   3360

cacacaaacc gatcttcgcc tcaaggaaac ctaattctac atccgagaga ctgccgagat   3420

ccagtctaca ctgattaatt ttcgggccaa taatttaaaa aaatcgtgtt atataatatt   3480

atatgtatta tatatataca tcatgatgat actgacagtc atgtcccatt gctaaataga   3540

cagactccat ctgccgcctc caactgatgt tctcaatatt taaggggtca tctcgcattg   3600

tttaataata aacagactcc atctaccgcc tccaaatgat gttctcaaaa tatattgtat   3660

gaacttattt ttattactta gtattattag acaacttact tgctttatga aaaacacttc   3720

ctatttagga aacaatttat aatggcagtt cgttcattta acaatttatg tagaataaat   3780

gttataaatg cgtatgggaa atcttaaata tggatagcat aaatgatatc tgcattgcct   3840

aattcgaaat caacagcaac gaaaaaaatc ccttgtacaa cataaatagt catcgagaaa   3900

tatcaactat caaagaacag ctattcacac gttactattg agattattat tggacgagaa   3960

tcacacactc aactgtcttt ctctcttcta gaaatacagg tacaagtatg tactattctc   4020

attgttcata cttctagtca tttcatccca catattcctt ggatttctct ccaatgaatg   4080

acattctatc ttgcaaattc aacaattata ataagatata ccaaagtagc ggtatagtgg   4140

caatcaaaaa gcttctctgg tgtgcttctc gtatttattt ttattctaat gatccattaa   4200

aggtatatat ttatttcttg ttatataatc cttttgttta ttacatgggc tggatacata   4260

aaggtatttt gatttaattt tttgcttaaa ttcaatcccc cctcgttcag tgtcaactgt   4320

aatggtagga aattaccata cttttgaaga agcaaaaaaa atgaaagaaa aaaaaaatcg   4380

tatttccagg ttagacgttc cgcagaatct agaatgcggt atgcggtaca ttgttcttcg   4440

aacgtaaaag ttgcgctccc tgagatattg tacatttttg cttttacaag tacaagtaca   4500

tcgtacaact atgtactact gttgatgcat ccacaacagt ttgttttgtt tttttttgtt   4560

tttttttttt ctaatgattc attaccgcta tgtataccta cttgtacttg tagtaagccg   4620

ggttattggc gttcaattaa tcatagactt atgaatctgc acggtgtgcg ctgcgagtta   4680

cttttagctt atgcatgcta cttgggtgta atattgggat ctgttcggaa atcaacggat   4740

gctcaatcga tttcgacagt aattaattaa gtcatacaca agtcagcttt cttcgagcct   4800

catataagta taagtagttc aacgtattag cactgtaccc agcatctccg tatcgagaaa   4860

cacaacaaca tgccccattg gacagatcat gcggatacac aggttgtgca gtatcataca   4920

tactcgatca gacaggtcgt ctgaccatca tacaagctga acaagcgctc catacttgca   4980

cgctctctat atacacagtt aaattacata tccatagtct aacctctaac agttaatctt   5040

ctggtaagcc tcccagccag ccttctggta tcgcttggcc tcctcaatag gatctcggtt   5100

ctggccgtac agacctcggc cgacaattat gatatccgtt ccggtagaca tgacatcctc   5160

aacagttcgg tactgctgtc cgagagcgtc tcccttgtcg tcaagaccca ccccgggggt   5220

cagaataagc cagtcctcag agtcgccctt aggtcggttc tgggcaatga agccaaccac   5280

aaactcgggg tcggatcggg caagctcaat ggtctgcttg gagtactcgc cagtggccag   5340

agagcccttg caagacagct cggccagcat gagcagacct ctggccagct tctcgttggg   5400

agaggggact aggaactcct tgtactggga gttctcgtag tcagagacgt cctccttctt   5460

ctgttcagag acagtttcct cggcaccagc tcgcaggcca gcaatgattc cggttccggg   5520

tacaccgtgg gcgttggtga tatcggacca ctcggcgatt cggtgacacc ggtactggtg   5580

cttgacagtg ttgccaatat ctgcgaactt tctgtcctcg aacaggaaga aaccgtgctt   5640

aagagcaagt tccttgaggg ggagcacagt gccggcgtag gtgaagtcgt caatgatgtc   5700

gatatgggtt ttgatcatgc acacataagg tccgacctta tcggcaagct caatgagctc   5760

cttggtggtg gtaacatcca gagaagcaca caggttggtt ttcttggctg ccacgagctt   5820

gagcactcga gcggcaaagg cggacttgtg gacgttagct cgagcttcgt aggagggcat   5880

tttggtggtg aagaggagac tgaaataaat ttagtctgca gaacttttta tcggaacctt   5940

atctggggca gtgaagtata tgttatggta atagttacga gttagttgaa cttatagata   6000

gactggacta tacggctatc ggtccaaatt agaaagaacg tcaatggctc tctgggcgtc   6060

gcctttgccg acaaaaatgt gatcatgatg aaagccagca atgacgttgc agctgatatt   6120

gttgtcggcc aaccgcgccg aaaacgcagc tgtcagaccc acagcctcca acgaagaatg   6180

tatcgtcaaa gtgatccaag cacactcata gttggagtcg tactccaaag gcggcaatga   6240

cgagtcagac agatactcgt cgaccgtacg gggagtttgg cgcccgtttt ttcgagcccc   6300

acacgtttcg gtgagtatga gcggcggcag attcgagcgt ttccggtttc cgcggctgga   6360

cgagagccca tgatgggggc tcccaccacc agcaatcagg gccctgatta cacacccacc   6420

tgtaatgtca tgctgttcat cgatggttaa tgctgctgtg tgctgtgtgt gtgtgttgtt   6480

tggcgctcat tgttgcgtta tgcagcgtac accacaatat tggaagctta ttagcctttc   6540

tattttttcg tttgcaaggc ttaacaacat tgctgtggag agggatgggg atatggaggc   6600

cgctggaggg agtcggagag gcgttttgga gcggcttggc ctggcgccca gctcgcgaaa   6660

cgcacctagg accctttggc acgccgaaat gtgccacttt tcagtctagt aacgccttac   6720

ctacgtcatt ccatgcgtgc atgtttgcgc cttttttccc ttgcccttga tcgccacaca   6780

gtacagtgca ctgtacagtg gaggttttgg gggggtctta gatgggagct aaaagcggcc   6840

tagcggtaca ctagtgggat tgtatggagt ggcatggagc ctaggtggag cctgacagga   6900

cgcacgaccg gctagcccgt gacagacgat gggtggctcc tgttgtccac cgcgtacaaa   6960

tgtttgggcc aaagtcttgt cagccttgct tgcgaaccta attcccaatt ttgtcacttc   7020

gcacccccat tgatcgagcc ctaacccctg cccatcaggc aatccaatta agctcgcatt   7080

gtctgccttg tttagtttgg ctcctgcccg tttcggcgtc cacttgcaca aacacaaaca   7140

agcattatat ataaggctcg tctctccctc ccaaccacac tcactttttt gcccgtcttc   7200

ccttgctaac acaaaagtca agaacacaaa caaccacccc aaccccctta cacacaagac   7260

atatctacag caatggc                                                  7277


<210>  156
<211>  7559
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pPEX10-1

<400>  156
gtacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca     60

ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat    120

taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc    180

tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca    240

aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca    300

aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg    360

ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg    420

acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt    480

ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt    540

tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc    600

tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt    660

gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt    720

agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc    780

tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa    840

agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt    900

tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct    960

acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta   1020

tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa   1080

agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc   1140

tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact   1200

acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc   1260

tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt   1320

ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta   1380

agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg   1440

tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt   1500

acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc   1560

agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt   1620

actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc   1680

tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc   1740

gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa   1800

ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac   1860

tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa   1920

aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt   1980

tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa   2040

tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct   2100

gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc   2160

gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc   2220

acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt   2280

agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg   2340

ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt   2400

ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta   2460

taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt   2520

aacgcgaatt ttaacaaaat attaacgctt acaatttcca ttcgccattc aggctgcgca   2580

actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg   2640

gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta   2700

aaacgacggc cagtgaattg taatacgact cactataggg cgaattgggt accgggcccc   2760

ccctcgaggt cgatggtgtc gataagcttg atatcgaatt catgtcacac aaaccgatct   2820

tcgcctcaag gaaacctaat tctacatccg agagactgcc gagatccagt ctacactgat   2880

taattttcgg gccaataatt taaaaaaatc gtgttatata atattatatg tattatatat   2940

atacatcatg atgatactga cagtcatgtc ccattgctaa atagacagac tccatctgcc   3000

gcctccaact gatgttctca atatttaagg ggtcatctcg cattgtttaa taataaacag   3060

actccatcta ccgcctccaa atgatgttct caaaatatat tgtatgaact tatttttatt   3120

acttagtatt attagacaac ttacttgctt tatgaaaaac acttcctatt taggaaacaa   3180

tttataatgg cagttcgttc atttaacaat ttatgtagaa taaatgttat aaatgcgtat   3240

gggaaatctt aaatatggat agcataaatg atatctgcat tgcctaattc gaaatcaaca   3300

gcaacgaaaa aaatcccttg tacaacataa atagtcatcg agaaatatca actatcaaag   3360

aacagctatt cacacgttac tattgagatt attattggac gagaatcaca cactcaactg   3420

tctttctctc ttctagaaat acaggtacaa gtatgtacta ttctcattgt tcatacttct   3480

agtcatttca tcccacatat tccttggatt tctctccaat gaatgacatt ctatcttgca   3540

aattcaacaa ttataataag atataccaaa gtagcggtat agtggcaatc aaaaagcttc   3600

tctggtgtgc ttctcgtatt tatttttatt ctaatgatcc attaaaggta tatatttatt   3660

tcttgttata taatcctttt gtttattaca tgggctggat acataaaggt attttgattt   3720

aattttttgc ttaaattcaa tcccccctcg ttcagtgtca actgtaatgg taggaaatta   3780

ccatactttt gaagaagcaa aaaaaatgaa agaaaaaaaa aatcgtattt ccaggttaga   3840

cgttccgcag aatctagaat gcggtatgcg gtacattgtt cttcgaacgt aaaagttgcg   3900

ctccctgaga tattgtacat ttttgctttt acaagtacaa gtacatcgta caactatgta   3960

ctactgttga tgcatccaca acagtttgtt ttgttttttt ttgttttttt tttttctaat   4020

gattcattac cgctatgtat acctacttgt acttgtagta agccgggtta ttggcgttca   4080

attaatcata gacttatgaa tctgcacggt gtgcgctgcg agttactttt agcttatgca   4140

tgctacttgg gtgtaatatt gggatctgtt cggaaatcaa cggatgctca atcgatttcg   4200

acagtaatta attaagtcat acacaagtca gctttcttcg agcctcatat aagtataagt   4260

agttcaacgt attagcactg tacccagcat ctccgtatcg agaaacacaa caacatgccc   4320

cattggacag atcatgcgga tacacaggtt gtgcagtatc atacatactc gatcagacag   4380

gtcgtctgac catcatacaa gctgaacaag cgctccatac ttgcacgctc tctatataca   4440

cagttaaatt acatatccat agtctaacct ctaacagtta atcttctggt aagcctccca   4500

gccagccttc tggtatcgct tggcctcctc aataggatct cggttctggc cgtacagacc   4560

tcggccgaca attatgatat ccgttccggt agacatgaca tcctcaacag ttcggtactg   4620

ctgtccgaga gcgtctccct tgtcgtcaag acccaccccg ggggtcagaa taagccagtc   4680

ctcagagtcg cccttaggtc ggttctgggc aatgaagcca accacaaact cggggtcgga   4740

tcgggcaagc tcaatggtct gcttggagta ctcgccagtg gccagagagc ccttgcaaga   4800

cagctcggcc agcatgagca gacctctggc cagcttctcg ttgggagagg ggactaggaa   4860

ctccttgtac tgggagttct cgtagtcaga gacgtcctcc ttcttctgtt cagagacagt   4920

ttcctcggca ccagctcgca ggccagcaat gattccggtt ccgggtacac cgtgggcgtt   4980

ggtgatatcg gaccactcgg cgattcggtg acaccggtac tggtgcttga cagtgttgcc   5040

aatatctgcg aactttctgt cctcgaacag gaagaaaccg tgcttaagag caagttcctt   5100

gagggggagc acagtgccgg cgtaggtgaa gtcgtcaatg atgtcgatat gggttttgat   5160

catgcacaca taaggtccga ccttatcggc aagctcaatg agctccttgg tggtggtaac   5220

atccagagaa gcacacaggt tggttttctt ggctgccacg agcttgagca ctcgagcggc   5280

aaaggcggac ttgtggacgt tagctcgagc ttcgtaggag ggcattttgg tggtgaagag   5340

gagactgaaa taaatttagt ctgcagaact ttttatcgga accttatctg gggcagtgaa   5400

gtatatgtta tggtaatagt tacgagttag ttgaacttat agatagactg gactatacgg   5460

ctatcggtcc aaattagaaa gaacgtcaat ggctctctgg gcgtcgcctt tgccgacaaa   5520

aatgtgatca tgatgaaagc cagcaatgac gttgcagctg atattgttgt cggccaaccg   5580

cgccgaaaac gcagctgtca gacccacagc ctccaacgaa gaatgtatcg tcaaagtgat   5640

ccaagcacac tcatagttgg agtcgtactc caaaggcggc aatgacgagt cagacagata   5700

ctcgtcgaca ttgtaactag tcctggaggg tcttttttat ggataacctc catgtacgat   5760

gtatccaaga tctccacgta ctgtgttctg tttcctaagt aatacccaac aacctctcca   5820

acaaacactt gggaagatgc acttgtgctg agatgtcaag atgttagtac tgtactggat   5880

ggagagaata ttaataaata attgttaccc aactacatct tgtcgattga aagagatacc   5940

cctaagacag ataggatatc tgcaacccga ggaatgaacc ccccagcacc ggcacccttt   6000

ctattaacaa aatgccaact gaaatttgaa aagttcaact aaacttattt gacccacaaa   6060

aactcgtcaa aagtggcggc gaaagctggc aaatgatgac atccccttgg aactatgata   6120

tcccctcgga atcttcgtcc ccatttgcca catctacttg caacgccacg tctgcttact   6180

aagcaaccca aatctgcctc ggctcaaaat gtggggaagt tcacatgcat tcgctggtga   6240

atctgatctg acactacaac tacacaccag gtccaacatg agcgacaata cgacaatcaa   6300

aaagccgatc cgacccaaac cgatccggac ggaacgcctg ccttacgctg gggccgcaga   6360

aatcatccga gccaaccaga aagaccacta ctttgagtcc gtgcttgaac agcatctcgt   6420

cacgtttctg cagaaatgga agggagtacg atttatccac cagtacaagg aggagctgga   6480

gacggcgtcc aagtttgcat atctcggttt gtgtacgctt gtgggctcca agactctcgg   6540

agaagagtac accaatctca tgtacactat cagagaccga acagctctac cgggggtggt   6600

gagacggttt ggctacgtgc tttccaacac tctgtttcca tacctgtttg tgcgctacat   6660

gggcaagttg cgcgccaaac tgatgcgcga gtatccccat ctggtggagt acgacgaaga   6720

tgagcctgtg cccagcccgg aaacatggaa ggagcgggtc atcaagacgt ttgtgaacaa   6780

gtttgacaag ttcacggcgc tggaggggtt taccgcgatc cacttggcga ttttctacgt   6840

ctacggctcg tactaccagc tcagtaagcg gatctggggc atgcgttatg tatttggaca   6900

ccgactggac aagaatgagc ctcgaatcgg ttacgagatg ctcggtctgc tgattttcgc   6960

ccggtttgcc acgtcatttg tgcagacggg aagagagtac ctcggagcgc tgctggaaaa   7020

gagcgtggag aaagaggcag gggagaagga agatgaaaag gaagcggttg tgccgaaaaa   7080

gaagtcgtca attccgttca ttgaggatac agaaggggag acggaagaca agatcgatct   7140

ggaggaccct cgacagctca agttcattcc tgaggcgtcc agagcgtgca ctctgtgtct   7200

gtcatacatt agtgcgccgg catgtacgcc atgtggacac tttttctgtt gggactgtat   7260

ttccgaatgg gtgagagaga agcccgagtg tcccttgtgt cggcagggtg tgagagagca   7320

gaacttgttg cctatcagat aatgacgagg tctggatgga aggactagtc agcgagacac   7380

agagcatcag ggaccagaca cgaccaattc aatcgacaac actgtgctgc atagcagtgc   7440

acagaggtcc tgggcatgaa tatattttag cattggagat atgagtggta gagcgtatac   7500

agtattaatt gtggaggtat ctcgtcgcat tgatagagca atacagttac tgctgaagc    7559


<210>  157
<211>  8051
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pPEX10-2

<400>  157
gtacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca     60

ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat    120

taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc    180

tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca    240

aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca    300

aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg    360

ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg    420

acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt    480

ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt    540

tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc    600

tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt    660

gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt    720

agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc    780

tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa    840

agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt    900

tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct    960

acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta   1020

tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa   1080

agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc   1140

tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact   1200

acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc   1260

tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt   1320

ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta   1380

agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg   1440

tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt   1500

acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc   1560

agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt   1620

actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc   1680

tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc   1740

gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa   1800

ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac   1860

tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa   1920

aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt   1980

tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa   2040

tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct   2100

gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc   2160

gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc   2220

acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt   2280

agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg   2340

ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt   2400

ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta   2460

taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt   2520

aacgcgaatt ttaacaaaat attaacgctt acaatttcca ttcgccattc aggctgcgca   2580

actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg   2640

gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta   2700

aaacgacggc cagtgaattg taatacgact cactataggg cgaattgggt accgggcccc   2760

ccctcgaggt cgatggtgtc gataagcttg atatcgaatt catgtcacac aaaccgatct   2820

tcgcctcaag gaaacctaat tctacatccg agagactgcc gagatccagt ctacactgat   2880

taattttcgg gccaataatt taaaaaaatc gtgttatata atattatatg tattatatat   2940

atacatcatg atgatactga cagtcatgtc ccattgctaa atagacagac tccatctgcc   3000

gcctccaact gatgttctca atatttaagg ggtcatctcg cattgtttaa taataaacag   3060

actccatcta ccgcctccaa atgatgttct caaaatatat tgtatgaact tatttttatt   3120

acttagtatt attagacaac ttacttgctt tatgaaaaac acttcctatt taggaaacaa   3180

tttataatgg cagttcgttc atttaacaat ttatgtagaa taaatgttat aaatgcgtat   3240

gggaaatctt aaatatggat agcataaatg atatctgcat tgcctaattc gaaatcaaca   3300

gcaacgaaaa aaatcccttg tacaacataa atagtcatcg agaaatatca actatcaaag   3360

aacagctatt cacacgttac tattgagatt attattggac gagaatcaca cactcaactg   3420

tctttctctc ttctagaaat acaggtacaa gtatgtacta ttctcattgt tcatacttct   3480

agtcatttca tcccacatat tccttggatt tctctccaat gaatgacatt ctatcttgca   3540

aattcaacaa ttataataag atataccaaa gtagcggtat agtggcaatc aaaaagcttc   3600

tctggtgtgc ttctcgtatt tatttttatt ctaatgatcc attaaaggta tatatttatt   3660

tcttgttata taatcctttt gtttattaca tgggctggat acataaaggt attttgattt   3720

aattttttgc ttaaattcaa tcccccctcg ttcagtgtca actgtaatgg taggaaatta   3780

ccatactttt gaagaagcaa aaaaaatgaa agaaaaaaaa aatcgtattt ccaggttaga   3840

cgttccgcag aatctagaat gcggtatgcg gtacattgtt cttcgaacgt aaaagttgcg   3900

ctccctgaga tattgtacat ttttgctttt acaagtacaa gtacatcgta caactatgta   3960

ctactgttga tgcatccaca acagtttgtt ttgttttttt ttgttttttt tttttctaat   4020

gattcattac cgctatgtat acctacttgt acttgtagta agccgggtta ttggcgttca   4080

attaatcata gacttatgaa tctgcacggt gtgcgctgcg agttactttt agcttatgca   4140

tgctacttgg gtgtaatatt gggatctgtt cggaaatcaa cggatgctca atcgatttcg   4200

acagtaatta attaagtcat acacaagtca gctttcttcg agcctcatat aagtataagt   4260

agttcaacgt attagcactg tacccagcat ctccgtatcg agaaacacaa caacatgccc   4320

cattggacag atcatgcgga tacacaggtt gtgcagtatc atacatactc gatcagacag   4380

gtcgtctgac catcatacaa gctgaacaag cgctccatac ttgcacgctc tctatataca   4440

cagttaaatt acatatccat agtctaacct ctaacagtta atcttctggt aagcctccca   4500

gccagccttc tggtatcgct tggcctcctc aataggatct cggttctggc cgtacagacc   4560

tcggccgaca attatgatat ccgttccggt agacatgaca tcctcaacag ttcggtactg   4620

ctgtccgaga gcgtctccct tgtcgtcaag acccaccccg ggggtcagaa taagccagtc   4680

ctcagagtcg cccttaggtc ggttctgggc aatgaagcca accacaaact cggggtcgga   4740

tcgggcaagc tcaatggtct gcttggagta ctcgccagtg gccagagagc ccttgcaaga   4800

cagctcggcc agcatgagca gacctctggc cagcttctcg ttgggagagg ggactaggaa   4860

ctccttgtac tgggagttct cgtagtcaga gacgtcctcc ttcttctgtt cagagacagt   4920

ttcctcggca ccagctcgca ggccagcaat gattccggtt ccgggtacac cgtgggcgtt   4980

ggtgatatcg gaccactcgg cgattcggtg acaccggtac tggtgcttga cagtgttgcc   5040

aatatctgcg aactttctgt cctcgaacag gaagaaaccg tgcttaagag caagttcctt   5100

gagggggagc acagtgccgg cgtaggtgaa gtcgtcaatg atgtcgatat gggttttgat   5160

catgcacaca taaggtccga ccttatcggc aagctcaatg agctccttgg tggtggtaac   5220

atccagagaa gcacacaggt tggttttctt ggctgccacg agcttgagca ctcgagcggc   5280

aaaggcggac ttgtggacgt tagctcgagc ttcgtaggag ggcattttgg tggtgaagag   5340

gagactgaaa taaatttagt ctgcagaact ttttatcgga accttatctg gggcagtgaa   5400

gtatatgtta tggtaatagt tacgagttag ttgaacttat agatagactg gactatacgg   5460

ctatcggtcc aaattagaaa gaacgtcaat ggctctctgg gcgtcgcctt tgccgacaaa   5520

aatgtgatca tgatgaaagc cagcaatgac gttgcagctg atattgttgt cggccaaccg   5580

cgccgaaaac gcagctgtca gacccacagc ctccaacgaa gaatgtatcg tcaaagtgat   5640

ccaagcacac tcatagttgg agtcgtactc caaaggcggc aatgacgagt cagacagata   5700

ctcgtcgacg tcttagcgtc atgtattctc aagcttagtc agagagaagg actatggagg   5760

agaaggggag aattgagaag ggtatttgaa gggactttga aggtcgcgtg gaagaggtac   5820

ttgaagaggt atttgaaggt cacgtggaag aggtatttga agatcacgtg gaagaagtac   5880

ttgttttaca gagaatatcg gggtgatttt gacagtggga ttgtctccca agtcctaatc   5940

gtttgacatg ggagcagtga aaagtcgggc taaaaaaggg aatatcggaa atcggaaaga   6000

cggaaagaat tactggactc atgtttagta gatctgagca cttcaaattt gaaaatatct   6060

cttcaaacag cagatcggtt ggtcgtggag gtaccatcaa gggtaaaatc aaggctatca   6120

tcaagggcca tatatcgcaa gtttggggga agataatatg ttcatagtga atcagggttg   6180

tggatttcct catctaacgg cattgtaact agtcctggag ggtctttttt atggataacc   6240

tccatgtacg atgtatccaa gatctccacg tactgtgttc tgtttcctaa gtaataccca   6300

acaacctctc caacaaacac ttgggaagat gcacttgtgc tgagatgtca agatgttagt   6360

actgtactgg atggagagaa tattaataaa taattgttac ccaactacat cttgtcgatt   6420

gaaagagata cccctaagac agataggata tctgcaaccc gaggaatgaa ccccccagca   6480

ccggcaccct ttctattaac aaaatgccaa ctgaaatttg aaaagttcaa ctaaacttat   6540

ttgacccaca aaaactcgtc aaaagtggcg gcgaaagctg gcaaatgatg acatcccctt   6600

ggaactatga tatcccctcg gaatcttcgt ccccatttgc cacatctact tgcaacgcca   6660

cgtctgctta ctaagcaacc caaatctgcc tcggctcaaa atgtggggaa gttcacatgc   6720

attcgctggt gaatctgatc tgacactaca actacacacc aggtccaaca tgagcgacaa   6780

tacgacaatc aaaaagccga tccgacccaa accgatccgg acggaacgcc tgccttacgc   6840

tggggccgca gaaatcatcc gagccaacca gaaagaccac tactttgagt ccgtgcttga   6900

acagcatctc gtcacgtttc tgcagaaatg gaagggagta cgatttatcc accagtacaa   6960

ggaggagctg gagacggcgt ccaagtttgc atatctcggt ttgtgtacgc ttgtgggctc   7020

caagactctc ggagaagagt acaccaatct catgtacact atcagagacc gaacagctct   7080

accgggggtg gtgagacggt ttggctacgt gctttccaac actctgtttc catacctgtt   7140

tgtgcgctac atgggcaagt tgcgcgccaa actgatgcgc gagtatcccc atctggtgga   7200

gtacgacgaa gatgagcctg tgcccagccc ggaaacatgg aaggagcggg tcatcaagac   7260

gtttgtgaac aagtttgaca agttcacggc gctggagggg tttaccgcga tccacttggc   7320

gattttctac gtctacggct cgtactacca gctcagtaag cggatctggg gcatgcgtta   7380

tgtatttgga caccgactgg acaagaatga gcctcgaatc ggttacgaga tgctcggtct   7440

gctgattttc gcccggtttg ccacgtcatt tgtgcagacg ggaagagagt acctcggagc   7500

gctgctggaa aagagcgtgg agaaagaggc aggggagaag gaagatgaaa aggaagcggt   7560

tgtgccgaaa aagaagtcgt caattccgtt cattgaggat acagaagggg agacggaaga   7620

caagatcgat ctggaggacc ctcgacagct caagttcatt cctgaggcgt ccagagcgtg   7680

cactctgtgt ctgtcataca ttagtgcgcc ggcatgtacg ccatgtggac actttttctg   7740

ttgggactgt atttccgaat gggtgagaga gaagcccgag tgtcccttgt gtcggcaggg   7800

tgtgagagag cagaacttgt tgcctatcag ataatgacga ggtctggatg gaaggactag   7860

tcagcgagac acagagcatc agggaccaga cacgaccaat tcaatcgaca acactgtgct   7920

gcatagcagt gcacagaggt cctgggcatg aatatatttt agcattggag atatgagtgg   7980

tagagcgtat acagtattaa ttgtggaggt atctcgtcgc attgatagag caatacagtt   8040

actgctgaag c                                                        8051


<210>  158
<211>  7966
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pYPS161

<400>  158
aaatgtaacg aaactgaaat ttgaccagat attgtgtccg cggtggagct ccagcttttg     60

ttccctttag tgagggttaa tttcgagctt ggcgtaatca tggtcatagc tgtttcctgt    120

gtgaaattgt tatccgctca caagcttcca cacaacgtac gttctggttg gctcggatga    180

tttctgcggc cccagcgtaa ggcaggcgtt ccgtccggat cggtttgggt cggatcggct    240

ttttgattgt cgtattgtcg ctcatgttgg acctggtgtg tagttgtagt gtcagatcag    300

attcaccagc gaatgcatgt gaacttcccc acattttgag ccgaggcaga tttgggttgc    360

ttagtaagca gacgtggcgt tgcaagtaga tgtggcaaat ggggacgaag attccgaggg    420

gatatcatag ttccaagggg atgtcatcat ttgccagctt tcgccgccac ttttgacgag    480

tttttgtggg tcaaataagt ttagttgaac ttttcaaatt tcagttggca ttttgttaat    540

agaaagggtg ccggtgctgg ggggttcatt cctcgggttg cagatatcct atctgtctta    600

ggggtatctc tttcaatcga caagatgtag ttgggtaaca attatttatt aatattctct    660

ccatccagta cagtactaac atcttgacat ctcagcacaa gtgcatcttc ccaagtgttt    720

gttggagagg ttgttgggta ttacttagga aacagaacac agtacgtgga gatcttggat    780

acatcgtaca tggaggttat ccataaaaaa gaccctccag gactagttac aatgccgtta    840

gatgaggaaa tccacaaccc tgattcacta tgaacatatt atcttccccc aaacttgcga    900

tatatggccc ttgatgatag ccttgatttt acccttgatg gtacctccac gaccaaccga    960

tctgctgttt gaagagatat tttcaaattt gaagtgctca gatctactaa acatgagtcc   1020

agtaattctt tccgtctttc cgatttccga tattcccttt tttagcccga cttttcactg   1080

ctcccatgtc aaacgattag gacttgggag acaatcccac tgtcaaaatc accccgatat   1140

tctctgtaaa acaagtactt cttccacgtg atcttcaaat acctcttcca cgtgaccttc   1200

aaatacctct tcaagtacct cttccacgcg accttcaaag tcccttcaaa tacccttctc   1260

aattctcccc ttctcctcca tagtccttct ctctgactaa gcttgagaat acatgacgct   1320

aagacgaaaa cacactagag accctgagag cctgaacatg catccactct gcagttgcgc   1380

acgtgcctac agcaactatc gggtccagtg ctggatctga cactgcgtct ccctatgaag   1440

aaactgataa acagatctgc actcataaca atgatctgag cgatgaaaac gtgacctcca   1500

cagccacaag tcataatcgg cgcgccagct gcattaatga atcggccaac gcgcggggag   1560

aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt   1620

cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga   1680

atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg   1740

taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa   1800

aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt   1860

tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct   1920

gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct   1980

cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc   2040

cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt   2100

atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc   2160

tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat   2220

ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa   2280

acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa   2340

aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga   2400

aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct   2460

tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga   2520

cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc   2580

catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg   2640

ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat   2700

aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat   2760

ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg   2820

caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc   2880

attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa   2940

agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc   3000

actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt   3060

ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag   3120

ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt   3180

gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag   3240

atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac   3300

cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc   3360

gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca   3420

gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg   3480

ggttccgcgc acatttcccc gaaaagtgcc acctgatgcg gtgtgaaata ccgcacagat   3540

gcgtaaggag aaaataccgc atcaggaaat tgtaagcgtt aatattttgt taaaattcgc   3600

gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc   3660

ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag   3720

tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga   3780

tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc   3840

actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa   3900

cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt   3960

agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc   4020

gtccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg   4080

ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca   4140

gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta   4200

tagggcgaat tgggcccgac gtcgcatgca actattagtg aggcttcggg agtggttgtc   4260

tcggttgtct cattcagact cgttgtgttg tatctatatc tatataaaca ctcttgtccc   4320

tcaatcccac tgccatcttt tgctaaactt gccgccaata tgaaactcat ctccctcatc   4380

accgtcgcta ccaccgctct ggcggctgtc ggagacaagt acaagctgac ctataccaga   4440

tcagacgccc aatcggtcga atctctgccc gtcacctacc aagatgacct gatcaccgcc   4500

tccaccgacg gcgaacccat caccatcacc gagggcgagg gcaacacctt ctctgttaac   4560

gacatgccca tcgcctatct ggagctgcag gctttgttct ggaccggcga ctacggctac   4620

aagctccagg gctcggtctt tgacattgcc gccgatggaa cctttgagct gagagacggc   4680

cccaaggagt actactattg cactcctcac cctgagcgaa acgtcatcta cgtcatcaac   4740

agccccgact actccaagtg tcggttcaag cgtaccatca agttccacgc tgaaaagatc   4800

taagtggtaa tcgaccgact aaccattttt agctgacaaa cacttgctaa ctcctataac   4860

gaatgaatga ctaacttggc atattgttac caagtattac ttgggatata gttgagtgta   4920

accattgcta agaatccaaa ctggagcttc taaaggtctg ggagtcgccg tatgtgttca   4980

tatcgaaatc aaagaaatca taatcgcaac agaattcaaa atcaagcaga ttaatatcca   5040

ttattgtact cggatcgtga catatctgat atgatctcgg atatgatctc tgactgttta   5100

ctgggagatt tgttgaagat ttgttgaggt tatctgaaaa gtagacaata gagacaaaat   5160

gacgatatca agaactgaat cgggccgaaa tactcggtat cattcccttc agcagtaact   5220

gtattgctct atcaatgcga cgagatacct ccacaattaa tactgtatac gctctaccac   5280

tcatatctcc aatgctaaaa tatattcatg cccaggacct ctgtgcactg ctatgcagca   5340

cagtgttgtc gattgaattg gtcgtgtctg gtccctgatg ctctgtgtct cgctgactag   5400

tccttccatc cagacctcgt cattatctga taggcaacaa gttctgctct ctcacaccct   5460

gccgacacaa gggacactcg ggcttctctc tcacccattc ggaaatacag tccttaatta   5520

agttgcgaca catgtcttga tagtatcttg aattctctct cttgagcttt tccataacaa   5580

gttcttctgc ctccaggaag tccatgggtg gtttgatcat ggttttggtg tagtggtagt   5640

gcagtggtgg tattgtgact ggggatgtag ttgagaataa gtcatacaca agtcagcttt   5700

cttcgagcct catataagta taagtagttc aacgtattag cactgtaccc agcatctccg   5760

tatcgagaaa cacaacaaca tgccccattg gacagatcat gcggatacac aggttgtgca   5820

gtatcataca tactcgatca gacaggtcgt ctgaccatca tacaagctga acaagcgctc   5880

catacttgca cgctctctat atacacagtt aaattacata tccatagtct aacctctaac   5940

agttaatctt ctggtaagcc tcccagccag ccttctggta tcgcttggcc tcctcaatag   6000

gatctcggtt ctggccgtac agacctcggc cgacaattat gatatccgtt ccggtagaca   6060

tgacatcctc aacagttcgg tactgctgtc cgagagcgtc tcccttgtcg tcaagaccca   6120

ccccgggggt cagaataagc cagtcctcag agtcgccctt aggtcggttc tgggcaatga   6180

agccaaccac aaactcgggg tcggatcggg caagctcaat ggtctgcttg gagtactcgc   6240

cagtggccag agagcccttg caagacagct cggccagcat gagcagacct ctggccagct   6300

tctcgttggg agaggggact aggaactcct tgtactggga gttctcgtag tcagagacgt   6360

cctccttctt ctgttcagag acagtttcct cggcaccagc tcgcaggcca gcaatgattc   6420

cggttccggg tacaccgtgg gcgttggtga tatcggacca ctcggcgatt cggtgacacc   6480

ggtactggtg cttgacagtg ttgccaatat ctgcgaactt tctgtcctcg aacaggaaga   6540

aaccgtgctt aagagcaagt tccttgaggg ggagcacagt gccggcgtag gtgaagtcgt   6600

caatgatgtc gatatgggtt ttgatcatgc acacataagg tccgacctta tcggcaagct   6660

caatgagctc cttggtggtg gtaacatcca gagaagcaca caggttggtt ttcttggctg   6720

ccacgagctt gagcactcga gcggcaaagg cggacttgtg gacgttagct cgagcttcgt   6780

aggagggcat tttggtggtg aagaggagac tgaaataaat ttagtctgca gaacttttta   6840

tcggaacctt atctggggca gtgaagtata tgttatggta atagttacga gttagttgaa   6900

cttatagata gactggacta tacggctatc ggtccaaatt agaaagaacg tcaatggctc   6960

tctgggcgtc gcctttgccg acaaaaatgt gatcatgatg aaagccagca atgacgttgc   7020

agctgatatt gttgtcggcc aaccgcgccg aaaacgcagc tgtcagaccc acagcctcca   7080

acgaagaatg tatcgtcaaa gtgatccaag cacactcata gttggagtcg tactccaaag   7140

gcggcaatga cgagtcagac agatactcgt cgaccttttc cttgggaacc accaccgtca   7200

gcccttctga ctcacgtatt gtagccaccg acacaggcaa cagtccgtgg atagcagaat   7260

atgtcttgtc ggtccatttc tcaccaactt taggcgtcaa gtgaatgttg cagaagaagt   7320

atgtgccttc attgagaatc ggtgttgctg atttcaataa agtcttgaga tcagtttggc   7380

cagtcatgtt gtggggggta attggattga gttatcgcct acagtctgta caggtatact   7440

cgctgcccac tttatacttt ttgattccgc tgcacttgaa gcaatgtcgt ttaccaaaag   7500

tgagaatgct ccacagaaca caccccaggg tatggttgag caaaaaataa acactccgat   7560

acggggaatc gaaccccggt ctccacggtt ctcaagaagt attcttgatg agagcgtatc   7620

gatgagccta aaatgaaccc gagtatatct cataaaattc tcggtgagag gtctgtgact   7680

gtcagtacaa ggtgccttca ttatgccctc aaccttacca tacctcactg aatgtagtgt   7740

acctctaaaa atgaaataca gtgccaaaag ccaaggcact gagctcgtct aacggacttg   7800

atatacaacc aattaaaaca aatgaaaaga aatacagttc tttgtatcat ttgtaacaat   7860

taccctgtac aaactaaggt attgaaatcc cacaatattc ccaaagtcca cccctttcca   7920

aattgtcatg cctacaactc atataccaag cactaaccta ccgttt                  7966


<210>  159
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Pex-10del1 3'.Forward

<400>  159
ccaacatgag cgacaatacg                                                 20


<210>  160
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Pex-10del2 5'.Reverse

<400>  160
caagttctgc tctctcacac                                                 20


<210>  161
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer KL2-3-1

<400>  161
ctcgaggatt cattcgagcc atg                                             23


<210>  162
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer KL2-3-2

<400>  162
cgagggagct ggatggtcca ag                                              22


<210>  163
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer SCP-5-2

<400>  163
atgtctctca aggtcgacgg cttc                                            24


<210>  164
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer KD2-5-3

<400>  164
gtgacttgtc tgttgcctgt tg                                              22


<210>  165
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 79-5-POX-1

<400>  165
gccatcaccg tcggagagac ag                                              22


<210>  166
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 79-5-POX-2

<400>  166
cgttgctgca cgtggaattt tc                                              22


<210>  167
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 4305ZP3-3-2

<400>  167
gttagtgaag tcttttatag                                                 20


<210>  168
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 79-3-POX-3

<400>  168
gtagggcaag agatgacaaa aag                                             23


<210>  169
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer KL2-5-2

<400>  169
gtacgttatc atttgaacag tg                                              22


<210>  170
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer KL2-5-3

<400>  170
ctcacaccat cctcattcaa ag                                              22


<210>  171
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer KL2-3-2

<400>  171
gactctgtga ttgagcagta tc                                              22


<210>  172
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer KL2-3-3

<400>  172
ctggtgaggt ctttattgac tg                                              22


<210>  173
<211>  844
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  chimeric fragment comprising DNA from chromosome E, unknown DNA 
       and 5'-end of pZKD2-5U89A2


<220>
<221>  misc_feature
<222>  (797)..(797)
<223>  n is a, c, g, or t

<400>  173
gtgacttgtc tgttggctgt tgttagaaga aaaagtggga gcttatcagt cacggtccac     60

gaacgatttc gtacttgtac gtaattggtc gtgagaactg ttgcagagcc ggtgcttttt    120

tttgtggcca agtcgacagg tcgatttcgg cgctgtgcga ggttgctggg atgtgctggt    180

ttggctacca aatgtgggga agatttcaac ctcggatttg acgtgtgtag agggcataac    240

acattattat agacgtgtct ctctcctttc ctaagggatc ttagagggat cttagaatac    300

cgcagtactg ttccttttcc actcaaagcg ggtctccaga acagccaggg ataaaataac    360

actatgatta acttgaaacg atctcatcca catcatcaaa ttggctgagc cagccttgaa    420

taaaagctga tatgctgaga agttgacact caacttgcca gtataaagtt atttcttatt    480

tgcttaacat cagtgtgtga atgaaggcca gatccagagt tttcaataac aaagaagact    540

tgttgccctt gccgacgtca ccgtcgttct tgaggtcaag gtaccaagac tcggtcttgc    600

cagccttgtt cttgaggttg aagacaatga tagcgttggc cttcttgatg gactcagcct    660

tctgcttggc ctgagagggg tcgttaagac cgtcacggat gacgtcgaag atgatagaag    720

aagtgaagcc gtcgaccttg agagacattg ttgtttttgt gtaatgaata agagatattc    780

tgggagctaa accggangat gcaccctacc tatatatatg tcgttagatt gactatatgc    840

attt                                                                 844


<210>  174
<211>  303
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  unknown DNA between the 5'-end of pZKD2-5U89A2 and chromosome E

<400>  174
gcataacaca ttattataga cgtgtctctc tcctttccta agggatctta gagggatctt     60

agaataccgc agtactgttc cttttccact caaagcgggt ctccagaaca gccagggata    120

aaataacact atgattaact tgaaacgatc tcatccacat catcaaattg gctgagccag    180

ccttgaataa aagctgatat gctgagaagt tgacactcaa cttgccagta taaagttatt    240

tcttatttgc ttaacatcag tgtgtgaatg aaggccagat ccagagtttt caataacaaa    300

gaa                                                                  303


<210>  175
<211>  2365
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  chimeric fragment comprising chromosome F, unknown DNA and 5'-end
       of pZP3-PA777U


<220>
<221>  misc_feature
<222>  (1403)..(1403)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (1439)..(1439)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (1686)..(1686)
<223>  n is a, c, g, or t

<400>  175
cgttgctgca cgtggaattt tcggccgaaa acctgtgggg tacttacttt tggcactgga     60

gagaagcatc tgggattttg ggaacctagg cagaagatga ggaaaaaaat aagaggaacc    120

gttgtgagct tgcttatcag tgtcatatac tcccccctcc ttgcgttttt gcgtcttttc    180

cccctatttt tccgattttg cgattttttt tctctttttt tccgcttttt tccgcttttt    240

ttttggccgg cttttatcca tttctccaag ccgaggatca catctatgca gcccagtccg    300

ttggagcata tctgcggtag agtttcggaa cggcgttaag cactgtgtcc gggtcggtct    360

ggaacgatat tgagcgggaa attcggggga ataagaccac cgttggactc cccgcaatga    420

ggagatcaag atgtgctttt cacaattctg attggtggcg ctctattttt gatcctgtgt    480

gggattattc accctgtatg ttgggttgac tgtgttgttt tgattgccgg agaattaaac    540

tgtcgaatca cttaacctgc tctctgcgcc tgattccaac caccttgaga atacgtgaca    600

gtaaggcaga cattttatac cgtactctaa tacgttattt agttgtattg ttaggcagac    660

attttatacc atactctaaa tgttatttag ttgtagtgtt tagcagacat tttataccat    720

actataatat gttatttagt tgcagtgttt tgcagacatt ttatacaata ctataatacg    780

ttatttagtt gtagtgtttg tgccaggggc ctctatggcc tccatcaggc gggctcctgc    840

atcccgtgtc tctgctcgcc tgacgggacc gtacctgggt ctgtgtgtga cccagagact    900

ggacagtgtg tgtgtaaggt gagtgtgtga gaatatgttt actgacatct attactgata    960

attatcttat ctactagtca ttttttatat atcacttagg aaaacactga gggacctcgc   1020

tgcgactcgt gccgccgtgg ttaccacagc ctggagagga ggaactcctt ggggtgtctg   1080

gcgtgtgcgt gtgacgtccg cggcaccctg aaggggggtg tgtgtgaccc cgtcaactcc   1140

cagtgtccct gtagggaggg agtggagggt gcccagtgta cacgctgctc cctacactac   1200

tacaacacaa cctctgaact ctacgatgac ctcacacctg acccctacta caacatgacc   1260

tttgaccccc aggggtcatc ccagggatgt gttccgtgtg tgtgtgaccc cacgggaaca   1320

gtggaggggg cgatgtgtga cgcggacacc gggcagtgtg tgtgtgtgcc cacgcgccac   1380

ggaagggatt gcggtagatg tangccaggt gagtgtgtgt gtgtgtgtgt gtccactcnc   1440

catggaaggg actgcggtag atgtaggcca ggtaagtgtg tgtgtgtgtg tgtgtgtgtg   1500

tgtgtgtgtg tgtgtgtgct tatgtttgtt cataaatcta cccaactggt ttgcaatggg   1560

tagaagtgct tagcaacaga atgttctctt actcacacct ttaactctgt ggacagaatg   1620

tttgtgttta tctctcttac tcacaccttt aactctgtgg acagaatgtt tgtggtttat   1680

ctctcnttac tcacaccttt aactctgtag gagaagatat gtatatgaca gtaatacttt   1740

atacttactg ttgaataagt atcgacgcac acaactccca gctcattaca ctgaagcatc   1800

tcagctctgg cattgacgac aagctgccat gctgtttacc acaatgctca acacactcct   1860

ctttcactcc ctttgttcct gtctctctct ttcaaatcac accctcggtc tgttcttttc   1920

tctcggtctg atggatggat ggtccctgta gtgtgtcttt ttgtttcttg gctcctggcg   1980

gtgttgtcgt gtgcctgcgt atgtgtgcgt atgtgtgtgt atgtgtgtat atgtgtgtgt   2040

atgtgtttgt gtgtgcgtgt gtatgcgtgt gtgtgtgtgt gtgtatgcat gtgtgtgtgc   2100

gtatgcatgt gtatgtgtgt gtgcctattt gtgtgtgtgt ctgtgctttg tcctggggaa   2160

tgatgatgag tgcttggcat tcactgtccg gctggagcca cccaaaagca gaaaaacgaa   2220

aaacctaggc tcagtgtgtg gtgtggaaag aaatggcgca ctagtgggca gcacagatag   2280

ccatcgagaa cagcaggata tccccctgtg tgtcgcggac ccgtcccaga ccagcccggg   2340

ccgtcgacca cgcgtgccct atagt                                         2365


<210>  176
<211>  1729
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Unknown DNA between the 5'-end of pZP3-PA777U and chromosome F


<220>
<221>  misc_feature
<222>  (947)..(947)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (983)..(983)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (1230)..(1230)
<223>  n is a, c, g, or t

<400>  176
ggcgctctat ttttgatcct gtgtgggatt attcaccctg tatgttgggt tgactgtgtt     60

gttttgattg ccggagaatt aaactgtcga atcacttaac ctgctctctg cgcctgattc    120

caaccacctt gagaatacgt gacagtaagg cagacatttt ataccgtact ctaatacgtt    180

atttagttgt attgttaggc agacatttta taccatactc taaatgttat ttagttgtag    240

tgtttagcag acattttata ccatactata atatgttatt tagttgcagt gttttgcaga    300

cattttatac aatactataa tacgttattt agttgtagtg tttgtgccag gggcctctat    360

ggcctccatc aggcgggctc ctgcatcccg tgtctctgct cgcctgacgg gaccgtacct    420

gggtctgtgt gtgacccaga gactggacag tgtgtgtgta aggtgagtgt gtgagaatat    480

gtttactgac atctattact gataattatc ttatctacta gtcatttttt atatatcact    540

taggaaaaca ctgagggacc tcgctgcgac tcgtgccgcc gtggttacca cagcctggag    600

aggaggaact ccttggggtg tctggcgtgt gcgtgtgacg tccgcggcac cctgaagggg    660

ggtgtgtgtg accccgtcaa ctcccagtgt ccctgtaggg agggagtgga gggtgcccag    720

tgtacacgct gctccctaca ctactacaac acaacctctg aactctacga tgacctcaca    780

cctgacccct actacaacat gacctttgac ccccaggggt catcccaggg atgtgttccg    840

tgtgtgtgtg accccacggg aacagtggag ggggcgatgt gtgacgcgga caccgggcag    900

tgtgtgtgtg tgcccacgcg ccacggaagg gattgcggta gatgtangcc aggtgagtgt    960

gtgtgtgtgt gtgtgtccac tcnccatgga agggactgcg gtagatgtag gccaggtaag   1020

tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgcttatgtt tgttcataaa   1080

tctacccaac tggtttgcaa tgggtagaag tgcttagcaa cagaatgttc tcttactcac   1140

acctttaact ctgtggacag aatgtttgtg tttatctctc ttactcacac ctttaactct   1200

gtggacagaa tgtttgtggt ttatctctcn ttactcacac ctttaactct gtaggagaag   1260

atatgtatat gacagtaata ctttatactt actgttgaat aagtatcgac gcacacaact   1320

cccagctcat tacactgaag catctcagct ctggcattga cgacaagctg ccatgctgtt   1380

taccacaatg ctcaacacac tcctctttca ctccctttgt tcctgtctct ctctttcaaa   1440

tcacaccctc ggtctgttct tttctctcgg tctgatggat ggatggtccc tgtagtgtgt   1500

ctttttgttt cttggctcct ggcggtgttg tcgtgtgcct gcgtatgtgt gcgtatgtgt   1560

gtgtatgtgt gtatatgtgt gtgtatgtgt ttgtgtgtgc gtgtgtatgc gtgtgtgtgt   1620

gtgtgtgtat gcatgtgtgt gtgcgtatgc atgtgtatgt gtgtgtgcct atttgtgtgt   1680

gtgtctgtgc tttgtcctgg ggaatgatga tgagtgcttg gcattcact               1729


<210>  177
<211>  326
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  chimeric fragment comprising chromosome F and 3'-end of the 
       AscI/PacI fragment of pZP3-PA777U

<400>  177
gttagtgaag tcttttatag gcatcagcgc ccaattttat ccgtgcttgt ctcactcccg     60

cattgggttt cattacctgc gcttatccat tttgctgtca aagcaaagtt tccaggtgta    120

gagttcgagc ttgaaactca atccaaaaca gtggtttttc ggctcctgta gcgggaaggc    180

gtcgagatat cgacattgtt ccatctccag tttaacccca acttatcgag agtatttgtg    240

agacacgcaa taaatgaatt tataccaatc aaatccatat tctacgctgt ctacatatag    300

atactttttg tcatctcttg ccctac                                         326


<210>  178
<211>  519
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chimeric fragment comprising chromosome C and the 5'-end of the 
       AscI/SphI fragment of pZKL2-5U89GC

<400>  178
ctcacaccat cctcattcaa agcgttacac tgattacgcg cccatcaacg acagcatgag     60

gggactgctg atctgatcta atcaaatgac tacaaaaatc gcaataatga agagcaaacg    120

acaaaaaaga aacaggttaa ccaatcccgc ttcaatgtct caccacaatc cagcactgtt    180

tctcattacc tcctccctct aatttcagag ttgcatcagg gtccttgatt tgttctcttt    240

ggttctgacg agaaatttta atagatgtct tgctgaatta ttacaattct aacttagtag    300

ttctgttaga gggcaattta gaggggcctt gtggttttga tttacacgaa attgaataga    360

atggggacaa aaaattgaaa gctaggacaa ctcctgagac acagtccttc tctttttata    420

ccaaaaccca tctcgtacgc cctatctcag tgtcttttgg tcgacacttt accgcatcta    480

tagaccagcc cgggccgtcg accacgcgtg ccctatagt                           519


<210>  179
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  unknown DNA at the junction of chromosome C and 5'-end of the 
       AscI/SphI fragment of pZKL2-5U89GC

<400>  179
ttgttctctt tggttctgac gagaaatttt aatagatgtc ttgctgaatt attacaattc     60

taactt                                                                66


<210>  180
<211>  711
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  fragment containing DNA from chromosome C and the 3'-end of the 
       AscI/SphI fragment of pZKL2-5U89GC

<400>  180
ctggtgaggt ctttattgac tggcccctga tccaccctcc tctctccaac gttgtcatgt     60

gccagggcca gagcaataaa cagtgctctg ccggtaacac tctgctccag caggtcaatg    120

tgattggaaa ccatctgcag tacttcgtca ccgagggtgt ctgtggtatc taagctattt    180

atcactcttt acaacttcta cctcaactat ctactttaat aaatgaatat cgtttattct    240

ctatgattac tgtatatgcg ttcctctaaa ctaaacaaca cagctaaaca acacagctaa    300

acaacacagc taaacaacac agctaacaca tgcatctact acaactaagt tgttcatcat    360

caactttcat ctttcaacac gatacaccac acaacatgtc cacgcgagac accttttacg    420

ggtggtattt gtgagtacaa cacggcgaac acacggtcta ccgcagacta tctacgctcc    480

gctccctgct aacatagtgc catccacttt gtcatcaccc tgatggtcga tggaggcttt    540

gccattcctg cggcgtacaa actacccggg caacagttca tggaagactt ccacgtggca    600

tccaacaagg atttcattgt gctcgaaaaa ccagcgtggt tcatgtcctt catctggatc    660

gagatcctct tccagaccag cccgggccgt cgaccacgcg tgccctatag t             711


<210>  181
<211>  65
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  unknown DNA at the junction of chromosome C and the 3'-end of the
       AscI/SphI fragment of pZKL2-5U89GC

<400>  181
ctaaactaaa caacacagct aaacaacaca gctaaacaac acagctaaac aacacagcta     60

acaca                                                                 65


<210>  182
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HPGG motif

<400>  182

His Pro Gly Gly 
1               


<210>  183
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HXGG motif


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid

<400>  183

His Xaa Gly Gly 
1               


<210>  184
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HPGX motif


<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  Xaa can be any naturally occurring amino acid

<400>  184

His Pro Gly Xaa 
1               


<210>  185
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HGGG motif

<400>  185

His Gly Gly Gly 
1               


<210>  186
<211>  4
<212>  PRT
<213>  Artificial Sequence 

<220>
<223>  HHGG motif

<400>  186

His His Gly Gly 
1               


<210>  187
<211>  4
<212>  PRT
<213>  Artificial Sequence 

<220>
<223>  HPGS motif

<400>  187

His Pro Gly Ser 
1               


<210>  188
<211>  4
<212>  PRT
<213>  Artificial Sequence 

<220>
<223>  HCGG motif

<400>  188

His Cys Gly Gly 
1               


<210>  189
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HWGG motif

<400>  189

His Trp Gly Gly 
1               


<210>  190
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His-rich motif


<220>
<221>  misc_feature
<222>  (2)..(4)
<223>  Xaa can be any naturally occurring amino acid

<400>  190

His Xaa Xaa Xaa His 
1               5   


<210>  191
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His-rich motif


<220>
<221>  misc_feature
<222>  (2)..(5)
<223>  Xaa can be any naturally occurring amino acid

<400>  191

His Xaa Xaa Xaa Xaa His 
1               5       


<210>  192
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His-rich motif


<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Xaa can be any naturally occurring amino acid

<400>  192

His Xaa Xaa His His 
1               5   


<210>  193
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His-rich motif


<220>
<221>  misc_feature
<222>  (2)..(4)
<223>  Xaa can be any naturally occurring amino acid

<400>  193

His Xaa Xaa Xaa His His 
1               5       


<210>  194
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His-rich motif


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa = His [H] or Gln [Q]

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Xaa can be any naturally occurring amino acid

<400>  194

Xaa Xaa Xaa His His 
1               5   


<210>  195
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His-rich motif


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa = His [H] or Gln [Q]

<220>
<221>  misc_feature
<222>  (2)..(4)
<223>  Xaa can be any naturally occurring amino acid

<400>  195

Xaa Xaa Xaa Xaa His His 
1               5       


<210>  196
<211>  1350
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  misc_feature
<222>  (1)..(1350)
<223>  synthetic mutant delta-5 desaturase

<400>  196
atggctctct cccttactac cgagcagctg ctcgagcgac ccgacctggt tgccatcgac     60

ggcattctct acgatctgga aggtcttgcc aaggtccatg gtggaggcga cttgatcctc    120

gcttctggtg cctccgatgc ttctcctctg ttctactcca tgcaccctta cgtcaagccc    180

gagaactcga agctgcttca acagttcgtg cgaggcaagc acgaccgaac ctccaaggac    240

attgtctaca cctacgactc tccctttgca caggacgtca agcgaactat gcgagaggtc    300

atgaaaggtc ggaactggta tgccacacct ggattctggc tgcgaaccgt tggcatcatt    360

gctgtcaccg ccttttgcga gtggcactgg gctactaccg gaatggtgct gtggggtctc    420

ttgactggat tcatgcacat gcagatcggc ctgtccattc agcacgatgc ctctcatggt    480

gccatcagca aaaagccctg ggtcaacgct ctctttgcct acggcatcga cgtcattgga    540

tcgtccagat ggatctggct gcagtctcac atcatgcgac atcacaccta caccaatcag    600

catggtctcg acctggatgc cgagtccgca gaaccattcc ttgtgttcca caactaccct    660

gctgccaaca ctgctcgaaa gtggtttcac cgattccagg cctggtacat gtacctcgtg    720

cttggagcct acggcgtttc gctggtgtac aaccctctct acatcttccg aatgcagcac    780

aacgacacca ttcccgagtc tgtcacagcc atgcgagaga acggctttct gcgacggtac    840

cgaacccttg cattcgttat gcgagctttc ttcatctttc gaaccgcctt cttgccctgg    900

tatctcactg gaacctccct gctcatcacc attcctctgg tgcccactgc taccggtgcc    960

ttcctcacct tctttttcat cttgtctcac aacttcgatg gctcggagcg aatccccgac   1020

aagaactgca aggtcaagag ctccgagaag gacgttgaag ccgatcagat cgactggtac   1080

agagctcagg tggagacctc ttccacctac ggtggaccca ttgccatgtt ctttactggc   1140

ggtctcaact tccagatcga gcatcacctc tttcctcgaa tgtcgtcttg gcactatccc   1200

ttcgtgcagc aagctgtccg agagtgttgc gaacgacacg gagttcggta cgtcttctac   1260

cctaccattg tgggcaacat catttccacc ctcaagtaca tgcacaaagt cggtgtggtt   1320

cactgtgtca aggacgctca ggattcctaa                                    1350


<210>  197
<211>  1350
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  misc_feature
<222>  (1)..(1350)
<223>  synthetic mutant delta-5 desaturase

<400>  197
atggctctct cccttactac cgagcagctg ctcgagcgac ccgacctggt tgccatcgac     60

ggcattctct acgatctgga aggtcttgcc aaggtccatc acggaggcga cttgatcctc    120

gcttctggtg cctccgatgc ttctcctctg ttctactcca tgcaccctta cgtcaagccc    180

gagaactcga agctgcttca acagttcgtg cgaggcaagc acgaccgaac ctccaaggac    240

attgtctaca cctacgactc tccctttgca caggacgtca agcgaactat gcgagaggtc    300

atgaaaggtc ggaactggta tgccacacct ggattctggc tgcgaaccgt tggcatcatt    360

gctgtcaccg ccttttgcga gtggcactgg gctactaccg gaatggtgct gtggggtctc    420

ttgactggat tcatgcacat gcagatcggc ctgtccattc agcacgatgc ctctcatggt    480

gccatcagca aaaagccctg ggtcaacgct ctctttgcct acggcatcga cgtcattgga    540

tcgtccagat ggatctggct gcagtctcac atcatgcgac atcacaccta caccaatcag    600

catggtctcg acctggatgc cgagtccgca gaaccattcc ttgtgttcca caactaccct    660

gctgccaaca ctgctcgaaa gtggtttcac cgattccagg cctggtacat gtacctcgtg    720

cttggagcct acggcgtttc gctggtgtac aaccctctct acatcttccg aatgcagcac    780

aacgacacca ttcccgagtc tgtcacagcc atgcgagaga acggctttct gcgacggtac    840

cgaacccttg cattcgttat gcgagctttc ttcatctttc gaaccgcctt cttgccctgg    900

tatctcactg gaacctccct gctcatcacc attcctctgg tgcccactgc taccggtgcc    960

ttcctcacct tctttttcat cttgtctcac aacttcgatg gctcggagcg aatccccgac   1020

aagaactgca aggtcaagag ctccgagaag gacgttgaag ccgatcagat cgactggtac   1080

agagctcagg tggagacctc ttccacctac ggtggaccca ttgccatgtt ctttactggc   1140

ggtctcaact tccagatcga gcatcacctc tttcctcgaa tgtcgtcttg gcactatccc   1200

ttcgtgcagc aagctgtccg agagtgttgc gaacgacacg gagttcggta cgtcttctac   1260

cctaccattg tgggcaacat catttccacc ctcaagtaca tgcacaaagt cggtgtggtt   1320

cactgtgtca aggacgctca ggattcctaa                                    1350


<210>  198
<211>  1350
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  misc_feature
<222>  (1)..(1350)
<223>  synthetic mutant delta-5 desaturase

<400>  198
atggctctct cccttactac cgagcagctg ctcgagcgac ccgacctggt tgccatcgac     60

ggcattctct acgatctgga aggtcttgcc aaggtccatc ccggatccga cttgatcctc    120

gcttctggtg cctccgatgc ttctcctctg ttctactcca tgcaccctta cgtcaagccc    180

gagaactcga agctgcttca acagttcgtg cgaggcaagc acgaccgaac ctccaaggac    240

attgtctaca cctacgactc tccctttgca caggacgtca agcgaactat gcgagaggtc    300

atgaaaggtc ggaactggta tgccacacct ggattctggc tgcgaaccgt tggcatcatt    360

gctgtcaccg ccttttgcga gtggcactgg gctactaccg gaatggtgct gtggggtctc    420

ttgactggat tcatgcacat gcagatcggc ctgtccattc agcacgatgc ctctcatggt    480

gccatcagca aaaagccctg ggtcaacgct ctctttgcct acggcatcga cgtcattgga    540

tcgtccagat ggatctggct gcagtctcac atcatgcgac atcacaccta caccaatcag    600

catggtctcg acctggatgc cgagtccgca gaaccattcc ttgtgttcca caactaccct    660

gctgccaaca ctgctcgaaa gtggtttcac cgattccagg cctggtacat gtacctcgtg    720

cttggagcct acggcgtttc gctggtgtac aaccctctct acatcttccg aatgcagcac    780

aacgacacca ttcccgagtc tgtcacagcc atgcgagaga acggctttct gcgacggtac    840

cgaacccttg cattcgttat gcgagctttc ttcatctttc gaaccgcctt cttgccctgg    900

tatctcactg gaacctccct gctcatcacc attcctctgg tgcccactgc taccggtgcc    960

ttcctcacct tctttttcat cttgtctcac aacttcgatg gctcggagcg aatccccgac   1020

aagaactgca aggtcaagag ctccgagaag gacgttgaag ccgatcagat cgactggtac   1080

agagctcagg tggagacctc ttccacctac ggtggaccca ttgccatgtt ctttactggc   1140

ggtctcaact tccagatcga gcatcacctc tttcctcgaa tgtcgtcttg gcactatccc   1200

ttcgtgcagc aagctgtccg agagtgttgc gaacgacacg gagttcggta cgtcttctac   1260

cctaccattg tgggcaacat catttccacc ctcaagtaca tgcacaaagt cggtgtggtt   1320

cactgtgtca aggacgctca ggattcctaa                                    1350


<210>  199
<211>  1365
<212>  DNA
<213>  Euglena anabaena UTEX 373


<220>
<221>  misc_feature
<222>  (1)..(1365)
<223>  synthetic mutant delta-5 desaturase

<400>  199
atggccacca tctccctgac taccgagcag ctcctggaac accccgagct cgttgccatc     60

gacggagtcc tgtacgatct cttcggtctg gccaaggtgc attgcggagg caacctcatc    120

gaagctgccg gtgcatccga cggaaccgct ctgttctact ccatgcatcc tggagtcaag    180

ccagagaact cgaagcttct gcagcaattt gcccgaggca agcacgaacg aagctccaag    240

gatcccgtgt acaccttcga ctctcccttt gctcaggacg tcaagcagtc cgttcgagag    300

gtcatgaagg gtcgaaactg gtacgccact cctggcttct ggctgagaac cgcactcatc    360

atcgcttgta ctgccattgg cgagtggtac tggatcacaa ccggagcagt gatgtggggt    420

atctttactg gatacttcca ctcgcagatt ggcttggcca ttcaacacga tgcttctcac    480

ggagccatca gcaaaaagcc ctgggtcaac gcctttttcg cttatggcat cgacgccatt    540

ggttcctctc gttggatctg gctgcagtcc cacattatgc gacatcacac ttacaccaac    600

cagcatggcc tcgacctgga tgctgcctcg gcagagccgt tcatcttgtt ccactcctat    660

cctgctacca acgcctctcg aaagtggtac caccgatttc aggcgtggta catgtacatc    720

gttctgggaa tgtatggtgt ctcgatggtg tacaatccca tgtacctctt cacaatgcag    780

cacaacgaca ccattcccga ggccacttct ctcagaccag gcagcttttt caatcggcag    840

cgagctttcg ccgtttccct tcgactgctc ttcatcttcc gaaacgcctt tcttccctgg    900

tacattgctg gtgcctctcc tctgctcacc attcttctgg tgcccacggt cacaggcatc    960

ttcctcacct ttgtgttcgt tctgtcccat aacttcgagg gagccgaacg gaccccagag   1020

aagaactgca aggccaaacg agctaaggaa ggcaaggagg tcagagacgt ggaagaggat   1080

cgagtcgact ggtaccgagc acaggccgag actgctgcca cctacggtgg cagcgtggga   1140

atgatgctta caggcggtct caacctgcag atcgagcatc acttgtttcc ccgaatgtcc   1200

tcttggcact atcccttcat tcaagacacc gttcgggagt gttgcaagcg acatggcgtc   1260

cgttacacat actatcctac cattctcgag aacatcatgt ccactcttcg atacatgcag   1320

aaggtgggtg ttgctcacac cattcaggat gcccaggagt tctaa                   1365


<210>  200
<211>  1392
<212>  DNA
<213>  Peridinium sp. CCMP626


<220>
<221>  misc_feature
<222>  (1)..(1392)
<223>  synthetic mutant delta-5 desaturase

<400>  200
atggctcccg acgccgacaa gctgcgacag cgaaaggctc agtccatcca ggacactgcc     60

gattctcagg ctaccgagct caagattggc accctgaagg gtctccaagg caccgagatc    120

gtcattgatg gcgacatcta cgacatcaaa gacttcgatc actgcggagg cgaatccatc    180

atgacctttg gtggcaacga cgttactgcc acctacaaga tgattcatcc ctaccactcg    240

aagcatcacc tggagaagat gaaaaaggtc ggtcgagtgc ccgactacac ctccgagtac    300

aagttcgata ctcccttcga acgagagatc aaacaggagg tcttcaagat tgtgcgaaga    360

ggtcgagagt ttggaacacc tggctacttc tttcgagcct tctgctacat cggtctcttc    420

ttttacctgc agtatctctg ggttaccact cctaccactt tcgcccttgc tatcttctac    480

ggtgtgtctc aggccttcat tggcctgaac gtccagcacg acgccaacca cggagctgcc    540

tccaaaaagc cctggatcaa caatttgctc ggcctgggtg ccgactttat cggaggctcc    600

aagtggctct ggatgaacca gcactggacc catcacactt acaccaacca tcacgagaag    660

gatcccgacg ccctgggtgc agagcctatg ctgctcttca acgactatcc cttgggtcac    720

cccaagcgaa ccctcattca tcacttccaa gccttctact atctgtttgt ccttgctggc    780

tactgggtgt cttcggtgtt caaccctcag atcctggacc tccagcaccg aggtgcccag    840

gctgtcggca tgaagatgga gaacgactac attgccaagt ctcgaaagta cgctatcttc    900

ctgcgactcc tgtacatcta caccaacatt gtggctccca tccagaacca aggcttttcg    960

ctcaccgtcg ttgctcacat tcttactatg ggtgtcgcct ccagcctgac cctcgctact   1020

ctgttcgccc tctcccacaa cttcgagaac gcagatcggg atcccaccta cgaggctcga   1080

aagggaggcg agcctgtctg ttggttcaag tcgcaggtgg aaacctcctc tacttacggt   1140

ggcttcattt ccggttgcct tacaggcgga ctcaactttc aggtcgagca tcacctgttt   1200

cctcgaatgt cctctgcctg gtacccctac atcgctccta ccgttcgaga ggtctgcaaa   1260

aagcacggcg tcaagtacgc ctactatccc tgggtgtggc agaacctcat ctcgaccgtc   1320

aagtacctgc atcagtccgg aactggctcg aactggaaga acggtgccaa tccctactct   1380

ggcaagctgt aa                                                       1392


<210>  201
<211>  1392
<212>  DNA
<213>  Peridinium sp. CCMP626


<220>
<221>  misc_feature
<222>  (1)..(1392)
<223>  synthetic mutant delta-5 desaturase

<400>  201
atggctcccg acgccgacaa gctgcgacag cgaaaggctc agtccatcca ggacactgcc     60

gattctcagg ctaccgagct caagattggc accctgaagg gtctccaagg caccgagatc    120

gtcattgatg gcgacatcta cgacatcaaa gacttcgatc actggggagg cgaatccatc    180

atgacctttg gtggcaacga cgttactgcc acctacaaga tgattcatcc ctaccactcg    240

aagcatcacc tggagaagat gaaaaaggtc ggtcgagtgc ccgactacac ctccgagtac    300

aagttcgata ctcccttcga acgagagatc aaacaggagg tcttcaagat tgtgcgaaga    360

ggtcgagagt ttggaacacc tggctacttc tttcgagcct tctgctacat cggtctcttc    420

ttttacctgc agtatctctg ggttaccact cctaccactt tcgcccttgc tatcttctac    480

ggtgtgtctc aggccttcat tggcctgaac gtccagcacg acgccaacca cggagctgcc    540

tccaaaaagc cctggatcaa caatttgctc ggcctgggtg ccgactttat cggaggctcc    600

aagtggctct ggatgaacca gcactggacc catcacactt acaccaacca tcacgagaag    660

gatcccgacg ccctgggtgc agagcctatg ctgctcttca acgactatcc cttgggtcac    720

cccaagcgaa ccctcattca tcacttccaa gccttctact atctgtttgt ccttgctggc    780

tactgggtgt cttcggtgtt caaccctcag atcctggacc tccagcaccg aggtgcccag    840

gctgtcggca tgaagatgga gaacgactac attgccaagt ctcgaaagta cgctatcttc    900

ctgcgactcc tgtacatcta caccaacatt gtggctccca tccagaacca aggcttttcg    960

ctcaccgtcg ttgctcacat tcttactatg ggtgtcgcct ccagcctgac cctcgctact   1020

ctgttcgccc tctcccacaa cttcgagaac gcagatcggg atcccaccta cgaggctcga   1080

aagggaggcg agcctgtctg ttggttcaag tcgcaggtgg aaacctcctc tacttacggt   1140

ggcttcattt ccggttgcct tacaggcgga ctcaactttc aggtcgagca tcacctgttt   1200

cctcgaatgt cctctgcctg gtacccctac atcgctccta ccgttcgaga ggtctgcaaa   1260

aagcacggcg tcaagtacgc ctactatccc tgggtgtggc agaacctcat ctcgaccgtc   1320

aagtacctgc atcagtccgg aactggctcg aactggaaga acggtgccaa tccctactct   1380

ggcaagctgt aa                                                       1392


