                         SEQUENCE LISTING

<110>  Archer-Daniels-Midland Company
       Binder, Thomas P.
       Hanke, Paul D.
 
<120>  Production of Amino Acids from Sucrose in Corynebacterium 
       glutamicum

<130>  060661

<150>  60/995348
<151>  2007-09-26

<160>  12    

<170>  PatentIn version 3.3

<210>  1
<211>  688
<212>  PRT
<213>  Corynebacterium glutamicum

<400>  1

Met Asn Ser Val Asn Asn Ser Ser Leu Val Arg Leu Asp Val Asp Phe 
1               5                   10                  15      


Gly Asp Ser Thr Thr Asp Val Ile Asn Asn Leu Ala Thr Val Ile Phe 
            20                  25                  30          


Asp Ala Gly Arg Ala Ser Ser Ala Asp Ala Leu Ala Lys Asp Ala Leu 
        35                  40                  45              


Asp Arg Glu Ala Lys Ser Gly Thr Gly Val Pro Gly Gln Val Ala Ile 
    50                  55                  60                  


Pro His Cys Arg Ser Glu Ala Val Ser Val Pro Thr Leu Gly Phe Ala 
65                  70                  75                  80  


Arg Leu Ser Lys Gly Val Asp Phe Ser Gly Pro Asp Gly Asp Ala Asn 
                85                  90                  95      


Leu Val Phe Leu Ile Ala Ala Pro Ala Gly Gly Gly Lys Glu His Leu 
            100                 105                 110         


Lys Ile Leu Ser Lys Leu Ala Arg Ser Leu Val Lys Lys Asp Phe Ile 
        115                 120                 125             


Lys Ala Leu Gln Glu Ala Thr Thr Glu Gln Glu Ile Val Asp Val Val 
    130                 135                 140                 


Asp Ala Val Leu Asn Pro Ala Pro Lys Thr Thr Glu Pro Ala Ala Ala 
145                 150                 155                 160 


Pro Ala Ala Ala Ala Val Ala Glu Ser Gly Ala Ala Ser Thr Ser Val 
                165                 170                 175     


Thr Arg Ile Val Ala Ile Thr Ala Cys Pro Thr Gly Ile Ala His Thr 
            180                 185                 190         


Tyr Met Ala Ala Asp Ser Leu Thr Gln Asn Ala Glu Gly Arg Asp Asp 
        195                 200                 205             


Val Glu Leu Val Val Glu Thr Gln Gly Ser Ser Ala Val Thr Pro Val 
    210                 215                 220                 


Asp Pro Lys Ile Ile Glu Ala Ala Asp Ala Val Ile Phe Ala Thr Asp 
225                 230                 235                 240 


Val Gly Val Lys Asp Arg Glu Arg Phe Ala Gly Lys Pro Val Ile Glu 
                245                 250                 255     


Ser Gly Val Lys Arg Ala Ile Asn Glu Pro Ala Lys Met Ile Asp Glu 
            260                 265                 270         


Ala Ile Ala Ala Ser Lys Asn Pro Asn Ala Arg Lys Val Ser Gly Ser 
        275                 280                 285             


Gly Val Ala Ala Ser Ala Glu Thr Thr Gly Glu Lys Leu Gly Trp Gly 
    290                 295                 300                 


Lys Arg Ile Gln Gln Ala Val Met Thr Gly Val Ser Tyr Met Val Pro 
305                 310                 315                 320 


Phe Val Ala Ala Gly Gly Leu Leu Leu Ala Leu Gly Phe Ala Phe Gly 
                325                 330                 335     


Gly Tyr Asp Met Ala Asn Gly Trp Gln Ala Ile Ala Thr Gln Phe Ser 
            340                 345                 350         


Leu Thr Asn Leu Pro Gly Asn Thr Val Asp Val Asp Gly Val Ala Met 
        355                 360                 365             


Thr Phe Glu Arg Ser Gly Phe Leu Leu Tyr Phe Gly Ala Val Leu Phe 
    370                 375                 380                 


Ala Thr Gly Gln Ala Ala Met Gly Phe Ile Val Ala Ala Leu Ser Gly 
385                 390                 395                 400 


Tyr Thr Ala Tyr Ala Leu Ala Gly Arg Pro Gly Ile Ala Pro Gly Phe 
                405                 410                 415     


Val Gly Gly Ala Ile Ser Val Thr Ile Gly Ala Gly Phe Ile Gly Gly 
            420                 425                 430         


Leu Val Thr Gly Ile Leu Ala Gly Leu Ile Ala Leu Trp Ile Gly Ser 
        435                 440                 445             


Trp Lys Val Pro Arg Val Val Gln Ser Leu Met Pro Val Val Ile Ile 
    450                 455                 460                 


Pro Leu Leu Thr Ser Val Val Val Gly Leu Val Met Tyr Leu Leu Leu 
465                 470                 475                 480 


Gly Arg Pro Leu Ala Ser Ile Met Thr Gly Leu Gln Asp Trp Leu Ser 
                485                 490                 495     


Ser Met Ser Gly Ser Ser Ala Ile Leu Leu Gly Ile Ile Leu Gly Leu 
            500                 505                 510         


Met Met Cys Phe Asp Leu Gly Gly Pro Val Asn Lys Ala Ala Tyr Leu 
        515                 520                 525             


Phe Gly Thr Ala Gly Leu Ser Thr Gly Asp Gln Ala Ser Met Glu Ile 
    530                 535                 540                 


Met Ala Ala Ile Met Ala Ala Gly Met Val Pro Pro Ile Ala Leu Ser 
545                 550                 555                 560 


Ile Ala Thr Leu Leu Arg Lys Lys Leu Phe Thr Pro Ala Glu Gln Glu 
                565                 570                 575     


Asn Gly Lys Ser Ser Trp Leu Leu Gly Leu Ala Phe Val Ser Glu Gly 
            580                 585                 590         


Ala Ile Pro Phe Ala Ala Ala Asp Pro Phe Arg Val Ile Pro Ala Met 
        595                 600                 605             


Met Ala Gly Gly Ala Thr Thr Gly Ala Ile Ser Met Ala Leu Gly Val 
    610                 615                 620                 


Gly Ser Arg Ala Pro His Gly Gly Ile Phe Val Val Trp Ala Ile Glu 
625                 630                 635                 640 


Pro Trp Trp Gly Trp Leu Ile Ala Leu Ala Ala Gly Thr Ile Val Ser 
                645                 650                 655     


Thr Ile Val Val Ile Ala Leu Lys Gln Phe Trp Pro Asn Lys Ala Val 
            660                 665                 670         


Ala Ala Glu Val Ala Lys Gln Glu Ala Gln Gln Ala Ala Val Asn Ala 
        675                 680                 685             


<210>  2
<211>  2067
<212>  DNA
<213>  Corynebacterium glutamicum

<400>  2
atgaatagcg taaataattc ctcgcttgtc cggctggatg tcgatttcgg cgactccacc     60

acggatgtca tcaacaacct tgccactgtt attttcgacg ctggccgagc ttcctccgcc    120

gacgcccttg ccaaagacgc gctggatcgt gaagcaaagt ccggcaccgg cgttcctggt    180

caagttgcta tcccccactg ccgttccgaa gccgtatctg tccctacctt gggctttgct    240

cgcctgagca agggtgtgga cttcagcgga cctgatggcg atgccaactt ggtgttcctc    300

attgcagcac ctgctggcgg cggcaaagag cacctgaaga tcctgtccaa gcttgctcgc    360

tccttggtga agaaggattt catcaaggct ctgcaggaag ccaccaccga gcaggaaatc    420

gtcgacgttg tcgatgccgt gctcaaccca gcaccaaaaa ccaccgagcc agctgcagct    480

ccggctgcgg cggcggttgc tgagagtggg gcggcgtcga caagcgttac tcgtatcgtg    540

gcaatcaccg catgcccaac cggtatcgca cacacctaca tggctgcgga ttccctgacg    600

caaaacgcgg aaggccgcga tgatgtggaa ctcgttgtgg agactcaggg ctcttccgct    660

gtcaccccag tcgatccgaa gatcatcgaa gctgccgacg ccgtcatctt cgccaccgac    720

gtgggagtta aagaccgcga gcgtttcgct ggcaagccag tcattgaatc cggcgtcaag    780

cgcgcgatca atgagccagc caagatgatc gacgaggcca tcgcagcctc caagaaccca    840

aacgcccgca aggtttccgg ttccggtgtc gcggcatctg ctgaaaccac cggcgagaag    900

ctcggctggg gcaagcgcat ccagcaggca gtcatgaccg gcgtgtccta catggttcca    960

ttcgtagctg ccggcggcct cctgttggct ctcggcttcg cattcggtgg atacgacatg   1020

gcgaacggct ggcaagcaat cgccacccag ttctctctga ccaacctgcc aggcaacacc   1080

gtcgatgttg acggcgtggc catgaccttc gagcgttcag gcttcctgtt gtacttcggc   1140

gcagtcctgt tcgccaccgg ccaagcagcc atgggcttca tcgtggcagc cctgtctggc   1200

tacaccgcat acgcacttgc tggacgccca ggcatcgcgc cgggcttcgt cggtggcgcc   1260

atctccgtca ccatcggcgc tggcttcatt ggtggtctgg ttaccggtat cttggctggt   1320

ctcattgccc tgtggattgg ctcctggaag gtgccacgcg tggtgcagtc actgatgcct   1380

gtggtcatca tcccgctact tacctcagtg gttgttggtc tcgtcatgta cctcctgctg   1440

ggtcgcccac tcgcatccat catgactggt ttgcaggact ggctatcgtc aatgtccgga   1500

agctccgcca tcttgctggg tatcatcttg ggcctcatga tgtgtttcga cctcggcgga   1560

ccagtaaaca aggcagccta cctctttggt accgcaggcc tgtctaccgg cgaccaagct   1620

tccatggaaa tcatggccgc gatcatggca gctggcatgg tcccaccaat cgcgttgtcc   1680

attgctaccc tgctgcgcaa gaagctgttc accccagcag agcaagaaaa cggcaagtct   1740

tcctggctgc ttggcctggc attcgtctcc gaaggtgcca tcccattcgc cgcagctgac   1800

ccattccgtg tgatcccagc aatgatggct ggcggtgcaa ccactggtgc aatctccatg   1860

gcactgggcg tcggctctcg ggctccacac ggcggtatct tcgtggtctg ggcaatcgaa   1920

ccatggtggg gctggctcat cgcacttgca gcaggcacca tcgtgtccac catcgttgtc   1980

atcgcactga agcagttctg gccaaacaag gccgtcgctg cagaagtcgc gaagcaagaa   2040

gcacaacaag cagctgtaaa cgcataa                                       2067


<210>  3
<211>  1167
<212>  DNA
<213>  Streptomyces rubiginosus 

<400>  3
atgaactacc agcccacccc cgaggacagg ttcaccttcg gactgtggac cgtcggctgg     60

cagggacggg accccttcgg tgacgccacg cggcgcgccc tcgacccggt cgagtcggtg    120

cggcggctgg ccgagctggg cgcccacggc gtcacgttcc acgacgacga cctcatcccc    180

ttcggctcca gcgacagcga gcgcgaggag cacgtcaagc ggttccggca ggcgctggac    240

gacaccggca tgaaggtgcc gatggccacc accaacctgt tcacccaccc ggtgttcaag    300

gacggcggct tcaccgccaa cgaccgcgac gtgcgccgct acgccctgcg caagaccatc    360

cgcaacatcg acctcgcggt cgagctcggc gccgagacct atgtggcctg gggcggccgc    420

gagggtgccg agtcgggtgg cgccaaggac gtgcgggacg ccctcgaccg catgaaggag    480

gccttcgacc tgctcggcga gtacgtcacc tcccagggct acgacatccg cttcgccatc    540

gagcccaagc cgaacgagcc gcgcggcgac atcctgctcc ccaccgtcgg ccacgccctg    600

gcgttcatcg agcgcctgga gcgaccggag ctgtacggcg tgaaccccga ggtcggccac    660

gagcagatgg ccgggctgaa cttcccgcac ggcatcgcgc aggcgctgtg ggcgggcaag    720

ctgttccaca tcgacctcaa cggccagaac ggcatcaagt acgaccagga cctccgcttc    780

ggcgcgggcg acctgcgggc cgcgttctgg ctggtggacc tgctggagtc ggccggctac    840

agcggcccgc ggcacttcga cttcaagccg ccgcggaccg aggacttcga cggggtgtgg    900

gcctcggcgg ccggctgcat gcgcaactac ctgatcctca aggagcgtgc ggcggccttc    960

cgcgccgacc ccgaggtgca ggaggcgctg cgcgcgtccc gtctggacga gctggcccgg   1020

cccacggcgg ccgacggtct gcaggccctg ctcgacgacc ggtccgcctt cgaggagttc   1080

gacgtcgacg cggcggcggc ccgtgggatg gccttcgagc gcctggacca gctggcgatg   1140

gaccacctgc tgggcgcccg gggctga                                       1167


<210>  4
<211>  388
<212>  PRT
<213>  Streptomyces rubiginosus

<400>  4

Met Asn Tyr Gln Pro Thr Pro Glu Asp Arg Phe Thr Phe Gly Leu Trp 
1               5                   10                  15      


Thr Val Gly Trp Gln Gly Arg Asp Pro Phe Gly Asp Ala Thr Arg Arg 
            20                  25                  30          


Ala Leu Asp Pro Val Glu Ser Val Arg Arg Leu Ala Glu Leu Gly Ala 
        35                  40                  45              


His Gly Val Thr Phe His Asp Asp Asp Leu Ile Pro Phe Gly Ser Ser 
    50                  55                  60                  


Asp Ser Glu Arg Glu Glu His Val Lys Arg Phe Arg Gln Ala Leu Asp 
65                  70                  75                  80  


Asp Thr Gly Met Lys Val Pro Met Ala Thr Thr Asn Leu Phe Thr His 
                85                  90                  95      


Pro Val Phe Lys Asp Gly Gly Phe Thr Ala Asn Asp Arg Asp Val Arg 
            100                 105                 110         


Arg Tyr Ala Leu Arg Lys Thr Ile Arg Asn Ile Asp Leu Ala Val Glu 
        115                 120                 125             


Leu Gly Ala Glu Thr Tyr Val Ala Trp Gly Gly Arg Glu Gly Ala Glu 
    130                 135                 140                 


Ser Gly Gly Ala Lys Asp Val Arg Asp Ala Leu Asp Arg Met Lys Glu 
145                 150                 155                 160 


Ala Phe Asp Leu Leu Gly Glu Tyr Val Thr Ser Gln Gly Tyr Asp Ile 
                165                 170                 175     


Arg Phe Ala Ile Glu Pro Lys Pro Asn Glu Pro Arg Gly Asp Ile Leu 
            180                 185                 190         


Leu Pro Thr Val Gly His Ala Leu Ala Phe Ile Glu Arg Leu Glu Arg 
        195                 200                 205             


Pro Glu Leu Tyr Gly Val Asn Pro Glu Val Gly His Glu Gln Met Ala 
    210                 215                 220                 


Gly Leu Asn Phe Pro His Gly Ile Ala Gln Ala Leu Trp Ala Gly Lys 
225                 230                 235                 240 


Leu Phe His Ile Asp Leu Asn Gly Gln Asn Gly Ile Lys Tyr Asp Gln 
                245                 250                 255     


Asp Leu Arg Phe Gly Ala Gly Asp Leu Arg Ala Ala Phe Trp Leu Val 
            260                 265                 270         


Asp Leu Leu Glu Ser Ala Gly Tyr Ser Gly Pro Arg His Phe Asp Phe 
        275                 280                 285             


Lys Pro Pro Arg Thr Glu Asp Phe Asp Gly Val Trp Ala Ser Ala Ala 
    290                 295                 300                 


Gly Cys Met Arg Asn Tyr Leu Ile Leu Lys Glu Arg Ala Ala Ala Phe 
305                 310                 315                 320 


Arg Ala Asp Pro Glu Val Gln Glu Ala Leu Arg Ala Ser Arg Leu Asp 
                325                 330                 335     


Glu Leu Ala Arg Pro Thr Ala Ala Asp Gly Leu Gln Ala Leu Leu Asp 
            340                 345                 350         


Asp Arg Ser Ala Phe Glu Glu Phe Asp Val Asp Ala Ala Ala Ala Arg 
        355                 360                 365             


Gly Met Ala Phe Glu Arg Leu Asp Gln Leu Ala Met Asp His Leu Leu 
    370                 375                 380                 


Gly Ala Arg Gly 
385             


<210>  5
<211>  323
<212>  PRT
<213>  Corynebacterium glutamicum

<400>  5

Met Pro Gln Lys Pro Ala Ser Phe Ala Val Gly Phe Asp Ile Gly Gly 
1               5                   10                  15      


Thr Asn Met Arg Ala Gly Leu Val Asp Glu Ser Gly Arg Ile Val Thr 
            20                  25                  30          


Ser Leu Ser Ala Pro Ser Pro Arg Thr Thr Gln Ala Met Glu Gln Gly 
        35                  40                  45              


Ile Phe Asp Leu Val Glu Gln Leu Lys Ala Glu Tyr Pro Val Gly Ala 
    50                  55                  60                  


Val Gly Leu Ala Val Ala Gly Phe Leu Asp Pro Glu Cys Glu Val Val 
65                  70                  75                  80  


Arg Phe Ala Pro His Leu Pro Trp Arg Asp Glu Pro Val Arg Glu Lys 
                85                  90                  95      


Leu Glu Asn Leu Leu Gly Leu Pro Val Arg Leu Glu His Asp Ala Asn 
            100                 105                 110         


Ser Ala Ala Trp Gly Glu His Arg Phe Gly Ala Ala Gln Gly Ala Asp 
        115                 120                 125             


Asn Trp Val Leu Leu Ala Leu Gly Thr Gly Ile Gly Ala Ala Leu Ile 
    130                 135                 140                 


Glu Lys Gly Glu Ile Tyr Arg Gly Ala Tyr Gly Thr Ala Pro Glu Phe 
145                 150                 155                 160 


Gly His Leu Arg Val Val Arg Gly Gly Arg Ala Cys Ala Cys Gly Lys 
                165                 170                 175     


Glu Gly Cys Leu Glu Arg Tyr Cys Ser Gly Thr Ala Leu Val Tyr Thr 
            180                 185                 190         


Ala Arg Glu Leu Ala Ser His Gly Ser Phe Arg Asn Ser Gly Leu Phe 
        195                 200                 205             


Asp Lys Ile Lys Ala Asp Pro Asn Ser Ile Asn Gly Lys Thr Ile Thr 
    210                 215                 220                 


Ala Ala Ala Arg Gln Glu Asp Pro Leu Ala Leu Ala Val Leu Glu Asp 
225                 230                 235                 240 


Phe Ser Glu Trp Leu Gly Glu Thr Leu Ala Ile Ile Ala Asp Val Leu 
                245                 250                 255     


Asp Pro Gly Met Ile Ile Ile Gly Gly Gly Leu Ser Asn Ala Ala Asp 
            260                 265                 270         


Leu Tyr Leu Asp Arg Ser Val Asn His Tyr Ser Thr Arg Ile Val Gly 
        275                 280                 285             


Ala Gly Tyr Arg Pro Leu Ala Arg Val Ala Thr Ala Gln Leu Gly Ala 
    290                 295                 300                 


Asp Ala Gly Met Ile Gly Val Ala Asp Leu Ala Arg Arg Ser Val Val 
305                 310                 315                 320 


Glu Ala Asn 
            


<210>  6
<211>  972
<212>  DNA
<213>  Corynebacterium glutamicum

<400>  6
atgccacaaa aaccggccag tttcgcggtg ggctttgaca tcggcggcac caacatgcga     60

gccgggcttg tcgacgaatc cgggcgcatc gtgaccagtt tgtcggcgcc gtcgccgcgc    120

acgacgcagg caatggaaca ggggattttt gatctagtcg aacagctcaa ggccgaatac    180

ccggttggtg ctgtgggact tgccgtcgcg ggatttttgg atcctgagtg cgaggttgtt    240

cgatttgccc cgcaccttcc ttggcgcgat gagccagtgc gtgaaaagtt ggaaaacctt    300

ttgggcctgc ctgttcgttt ggaacatgat gccaactcag cagcgtgggg tgagcatcgt    360

tttggtgcag ctcaaggcgc tgacaactgg gttttgttgg cactcggcac tggaattggt    420

gcagcgctga ttgaaaaagg cgaaatttac cgtggtgcat atggcacggc accagaattt    480

ggtcatttgc gtgttgttcg tggcggacgc gcatgtgcgt gtggcaaaga aggctgcctg    540

gagcgttact gttccggtac tgccttggtt tacactgcgc gtgaattggc ttcgcatggc    600

tcattccgca acagcgggct gtttgacaag atcaaagccg atccgaattc catcaatgga    660

aaaacgatca ctgcggcagc gcgccaagaa gacccacttg ctctcgccgt tctggaagat    720

ttcagcgagt ggctgggcga aactttggcg atcattgctg atgtccttga cccaggcatg    780

atcatcattg gtggcggact gtccaatgct gccgaccttt atttggatcg ctcggtcaac    840

cactattcca cccgcatcgt cggcgcagga tatcgccctt tggcacgcgt tgccacagct    900

cagttgggtg cggatgctgg catgatcggt gtcgctgatc tagctcgacg ctctgtagtg    960

gaagccaact ag                                                        972


<210>  7
<211>  28
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer

<400>  7
gcaagcttcc atcgcagcct ccaagaac                                        28


<210>  8
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  8
gcaagcttcg gagcttccgg acattgac                                        28


<210>  9
<211>  95
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR Primer

<400>  9
attgacaatt aatcatcggc tcgtataatg tgtggaattg tgagcggata acaatttcac     60

acaggaaaca gctatgaact accagcccac ccccg                                95


<210>  10
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  10
tcagccccgg gcgcccagc                                                  19


<210>  11
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  11
gaacgcgagg gggcactctt atgccacaaa aaccggcc                             38


<210>  12
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  12
ctagttggct tccactacag agcg                                            24


