                         SEQUENCE LISTING

<110>  Takeda Pharmaceutical Company Limited
 
<120>  POLO-LIKE KINASE INHIBITORS

<130>  PLK-5010-WO

<150>  60/975,127
<151>  2007-09-25

<150>  61/037,303
<151>  2007-03-17

<160>  6     

<170>  PatentIn version 3.3

<210>  1
<211>  1809
<212>  DNA
<213>  Homo sapiens

<400>  1
atgagtgctg cagtgactgc agggaagctg gcacgggcac cggccgaccc tgggaaagcc     60

ggggtccccg gagttgcagc tcccggagct ccggcggcgg ctccaccggc gaaagagatc    120

ccggaggtcc tagtggaccc acgcagccgg cggcgctatg tgcggggccg ctttttgggc    180

aagggcggct ttgccaagtg cttcgagatc tcggacgcgg acaccaagga ggtgttcgcg    240

ggcaagattg tgcctaagtc tctgctgctc aagccgcacc agagggagaa gatgtccatg    300

gaaatatcca ttcaccgcag cctcgcccac cagcacgtcg taggattcca cggctttttc    360

gaggacaacg acttcgtgtt cgtggtgttg gagctctgcc gccggaggtc tctcctggag    420

ctgcacaaga ggaggaaagc cctgactgag cctgaggccc gatactacct acggcaaatt    480

gtgcttggct gccagtacct gcaccgaaac cgagttattc atcgagacct caagctgggc    540

aaccttttcc tgaatgaaga tctggaggtg aaaatagggg attttggact ggcaaccaaa    600

gtcgaatatg acggggagag gaagaagacc ctgtgtggga ctcctaatta catagctccc    660

gaggtgctga gcaagaaagg gcacagtttc gaggtggatg tgtggtccat tgggtgtatc    720

atgtatacct tgttagtggg caaaccacct tttgagactt cttgcctaaa agagacctac    780

ctccggatca agaagaatga atacagtatt cccaagcaca tcaaccccgt ggccgcctcc    840

ctcatccaga agatgcttca gacagatccc actgcccgcc caaccattaa cgagctgctt    900

aatgacgagt tctttacttc tggctatatc cctgcccgtc tccccatcac ctgcctgacc    960

attccaccaa ggttttcgat tgctcccagc agcctggacc ccagcaaccg gaagcccctc   1020

acagtcctca ataaaggctt ggagaacccc ctgcctgagc gtccccggga aaaagaagaa   1080

ccagtggttc gagagacagg tgaggtggtc gactgccacc tcagtgacat gctgcagcag   1140

ctgcacagtg tcaatgcctc caagccctcg gagcgtgggc tggtcaggca agaggaggct   1200

gaggatcctg cctgcatccc catcttctgg gtcagcaagt gggtggacta ttcggacaag   1260

tacggccttg ggtatcagct ctgtgataac agcgtggggg tgctcttcaa tgactcaaca   1320

cgcctcatcc tctacaatga tggtgacagc ctgcagtaca tagagcgtga cggcactgag   1380

tcctacctca ccgtgagttc ccatcccaac tccttgatga agaagatcac cctccttaaa   1440

tatttccgca attacatgag cgagcacttg ctgaaggcag gtgccaacat cacgccgcgc   1500

gaaggtgatg agctcgcccg gctgccctac ctacggacct ggttccgcac ccgcagcgcc   1560

atcatcctgc acctcagcaa cggcagcgtg cagatcaact tcttccagga tcacaccaag   1620

ctcatcttgt gcccactgat ggcagccgtg acctacatcg acgagaagcg ggacttccgc   1680

acataccgcc tgagtctcct ggaggagtac ggctgctgca aggagctggc cagccggctc   1740

cgctacgccc gcactatggt ggacaagctg ctgagctcac gctcggccag caaccgtctc   1800

aaggcctcc                                                           1809


<210>  2
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  DNA sequence endoding PCR Primer


<220>
<221>  misc_feature
<222>  (1)..(27)
<223>  DNA sequence endoding PCR Primer

<400>  2
agtgctgcag tgactgcagg gaagctg                                         27


<210>  3
<211>  29
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic Primer


<220>
<221>  misc_feature
<222>  (1)..(29)
<223>  DNA sequence endoding PCR Primer

<400>  3
ttaggaggcc ttgagacggt tgctggccg                                       29


<210>  4
<211>  69
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic Primer


<220>
<221>  misc_feature
<222>  (1)..(69)
<223>  DAN sequence endoding PCT Primer

<400>  4
aaatctagag ccaccatgga ctacaaggac gacgatgaca agagtgctgc agtgactgca     60

gggaagctg                                                             69


<210>  5
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic Primer


<220>
<221>  misc_feature
<222>  (1)..(27)
<223>  DNA sequence endoding PCT Primer

<400>  5
tggcaactag aaggcacagt cgaggct                                         27


<210>  6
<211>  611
<212>  PRT
<213>  Artificial

<220>
<223>  Protein Sequence encoding FLAG-tagged PLK1


<220>
<221>  MISC_FEATURE
<222>  (1)..(9)
<223>  FLAG-tag

<220>
<221>  MISC_FEATURE
<222>  (10)..(611)
<223>  Human PLK1

<400>  6

Met Asp Tyr Lys Asp Asp Asp Asp Lys Ser Ala Ala Val Thr Ala Gly 
1               5                   10                  15      


Lys Leu Ala Arg Ala Pro Ala Asp Pro Gly Lys Ala Gly Val Pro Gly 
            20                  25                  30          


Val Ala Ala Pro Gly Ala Pro Ala Ala Ala Pro Pro Ala Lys Glu Ile 
        35                  40                  45              


Pro Glu Val Leu Val Asp Pro Arg Ser Arg Arg Arg Tyr Val Arg Gly 
    50                  55                  60                  


Arg Phe Leu Gly Lys Gly Gly Phe Ala Lys Cys Phe Glu Ile Ser Asp 
65                  70                  75                  80  


Ala Asp Thr Lys Glu Val Phe Ala Gly Lys Ile Val Pro Lys Ser Leu 
                85                  90                  95      


Leu Leu Lys Pro His Gln Arg Glu Lys Met Ser Met Glu Ile Ser Ile 
            100                 105                 110         


His Arg Ser Leu Ala His Gln His Val Val Gly Phe His Gly Phe Phe 
        115                 120                 125             


Glu Asp Asn Asp Phe Val Phe Val Val Leu Glu Leu Cys Arg Arg Arg 
    130                 135                 140                 


Ser Leu Leu Glu Leu His Lys Arg Arg Lys Ala Leu Thr Glu Pro Glu 
145                 150                 155                 160 


Ala Arg Tyr Tyr Leu Arg Gln Ile Val Leu Gly Cys Gln Tyr Leu His 
                165                 170                 175     


Arg Asn Arg Val Ile His Arg Asp Leu Lys Leu Gly Asn Leu Phe Leu 
            180                 185                 190         


Asn Glu Asp Leu Glu Val Lys Ile Gly Asp Phe Gly Leu Ala Thr Lys 
        195                 200                 205             


Val Glu Tyr Asp Gly Glu Arg Lys Lys Thr Leu Cys Gly Thr Pro Asn 
    210                 215                 220                 


Tyr Ile Ala Pro Glu Val Leu Ser Lys Lys Gly His Ser Phe Glu Val 
225                 230                 235                 240 


Asp Val Trp Ser Ile Gly Cys Ile Met Tyr Thr Leu Leu Val Gly Lys 
                245                 250                 255     


Pro Pro Phe Glu Thr Ser Cys Leu Lys Glu Thr Tyr Leu Arg Ile Lys 
            260                 265                 270         


Lys Asn Glu Tyr Ser Ile Pro Lys His Ile Asn Pro Val Ala Ala Ser 
        275                 280                 285             


Leu Ile Gln Lys Met Leu Gln Thr Asp Pro Thr Ala Arg Pro Thr Ile 
    290                 295                 300                 


Asn Glu Leu Leu Asn Asp Glu Phe Phe Thr Ser Gly Tyr Ile Pro Ala 
305                 310                 315                 320 


Arg Leu Pro Ile Thr Cys Leu Thr Ile Pro Pro Arg Phe Ser Ile Ala 
                325                 330                 335     


Pro Ser Ser Leu Asp Pro Ser Asn Arg Lys Pro Leu Thr Val Leu Asn 
            340                 345                 350         


Lys Gly Leu Glu Asn Pro Leu Pro Glu Arg Pro Arg Glu Lys Glu Glu 
        355                 360                 365             


Pro Val Val Arg Glu Thr Gly Glu Val Val Asp Cys His Leu Ser Asp 
    370                 375                 380                 


Met Leu Gln Gln Leu His Ser Val Asn Ala Ser Lys Pro Ser Glu Arg 
385                 390                 395                 400 


Gly Leu Val Arg Gln Glu Glu Ala Glu Asp Pro Ala Cys Ile Pro Ile 
                405                 410                 415     


Phe Trp Val Ser Lys Trp Val Asp Tyr Ser Asp Lys Tyr Gly Leu Gly 
            420                 425                 430         


Tyr Gln Leu Cys Asp Asn Ser Val Gly Val Leu Phe Asn Asp Ser Thr 
        435                 440                 445             


Arg Leu Ile Leu Tyr Asn Asp Gly Asp Ser Leu Gln Tyr Ile Glu Arg 
    450                 455                 460                 


Asp Gly Thr Glu Ser Tyr Leu Thr Val Ser Ser His Pro Asn Ser Leu 
465                 470                 475                 480 


Met Lys Lys Ile Thr Leu Leu Lys Tyr Phe Arg Asn Tyr Met Ser Glu 
                485                 490                 495     


His Leu Leu Lys Ala Gly Ala Asn Ile Thr Pro Arg Glu Gly Asp Glu 
            500                 505                 510         


Leu Ala Arg Leu Pro Tyr Leu Arg Thr Trp Phe Arg Thr Arg Ser Ala 
        515                 520                 525             


Ile Ile Leu His Leu Ser Asn Gly Ser Val Gln Ile Asn Phe Phe Gln 
    530                 535                 540                 


Asp His Thr Lys Leu Ile Leu Cys Pro Leu Met Ala Ala Val Thr Tyr 
545                 550                 555                 560 


Ile Asp Glu Lys Arg Asp Phe Arg Thr Tyr Arg Leu Ser Leu Leu Glu 
                565                 570                 575     


Glu Tyr Gly Cys Cys Lys Glu Leu Ala Ser Arg Leu Arg Tyr Ala Arg 
            580                 585                 590         


Thr Met Val Asp Lys Leu Leu Ser Ser Arg Ser Ala Ser Asn Arg Leu 
        595                 600                 605             


Lys Ala Ser 
    610     


