                         SEQUENCE LISTING

<110>  Knudsen, Nick
       Bussell, Stuart
       Kraynov, Vadim
 
<120>  Modified Human Apolipoprotein A-1 and Its Uses

<130>  AMBX-0141.00PCT

<150>  60/993,963
<151>  2007-09-14

<160>  26    

<170>  PatentIn version 3.4

<210>  1
<211>  729
<212>  DNA
<213>  Homo sapiens

<400>  1
gacgagccac cgcagagtcc atgggatcga gttaaagacc tggctaccgt ttacgtggac     60

gtcctgaaag actctggtcg tgactacgtg tctcagttcg aaggtagcgc tctgggtaaa    120

cagcttaacc tcaaactgct ggacaactgg gactccgtga cctccacctt cagcaaactg    180

cgtgaacagc ttggtccggt gactcaggaa ttttgggata acctcgaaaa ggaaaccgag    240

ggtctgcgtc aggaaatgtc taaagacctc gaagaagtta aagctaaagt gcagccgtac    300

ctggacgact tccaaaaaaa atggcaggag gaaatggagc tgtaccgtca gaaagttgaa    360

cctctgcgtg cggagctgca ggaaggtgcg cgtcagaaat tgcacgaact gcaggaaaaa    420

cttagcccgc tgggtgaaga aatgcgtgat cgtgctcgtg ctcacgttga tgctctgcgt    480

acccacctgg ctccgtatag cgatgaactg cgtcaacgtt tggcggctcg tctggaggct    540

ctgaaagaaa acggtggtgc gcgtctggct gagtaccacg ctaaagccac tgaacacctt    600

agcaccctga gcgaaaaagc taaaccggcg cttgaagacc tgcgtcaggg tctgctgccg    660

gttctggagt ccttcaaagt tagcttcctt agcgctctgg aggaatacac caaaaaactg    720

aacacccag                                                            729


<210>  2
<211>  243
<212>  PRT
<213>  Homo sapiens

<400>  2

Asp Glu Pro Pro Gln Ser Pro Trp Asp Arg Val Lys Asp Leu Ala Thr 
1               5                   10                  15      


Val Tyr Val Asp Val Leu Lys Asp Ser Gly Arg Asp Tyr Val Ser Gln 
            20                  25                  30          


Phe Glu Gly Ser Ala Leu Gly Lys Gln Leu Asn Leu Lys Leu Leu Asp 
        35                  40                  45              


Asn Trp Asp Ser Val Thr Ser Thr Phe Ser Lys Leu Arg Glu Gln Leu 
    50                  55                  60                  


Gly Pro Val Thr Gln Glu Phe Trp Asp Asn Leu Glu Lys Glu Thr Glu 
65                  70                  75                  80  


Gly Leu Arg Gln Glu Met Ser Lys Asp Leu Glu Glu Val Lys Ala Lys 
                85                  90                  95      


Val Gln Pro Tyr Leu Asp Asp Phe Gln Lys Lys Trp Gln Glu Glu Met 
            100                 105                 110         


Glu Leu Tyr Arg Gln Lys Val Glu Pro Leu Arg Ala Glu Leu Gln Glu 
        115                 120                 125             


Gly Ala Arg Gln Lys Leu His Glu Leu Gln Glu Lys Leu Ser Pro Leu 
    130                 135                 140                 


Gly Glu Glu Met Arg Asp Arg Ala Arg Ala His Val Asp Ala Leu Arg 
145                 150                 155                 160 


Thr His Leu Ala Pro Tyr Ser Asp Glu Leu Arg Gln Arg Leu Ala Ala 
                165                 170                 175     


Arg Leu Glu Ala Leu Lys Glu Asn Gly Gly Ala Arg Leu Ala Glu Tyr 
            180                 185                 190         


His Ala Lys Ala Thr Glu His Leu Ser Thr Leu Ser Glu Lys Ala Lys 
        195                 200                 205             


Pro Ala Leu Glu Asp Leu Arg Gln Gly Leu Leu Pro Val Leu Glu Ser 
    210                 215                 220                 


Phe Lys Val Ser Phe Leu Ser Ala Leu Glu Glu Tyr Thr Lys Lys Leu 
225                 230                 235                 240 


Asn Thr Gln 
            


<210>  3
<211>  243
<212>  PRT
<213>  Homo sapiens

<400>  3

Asp Glu Pro Pro Gln Ser Pro Trp Asp Arg Val Lys Asp Leu Ala Thr 
1               5                   10                  15      


Val Tyr Val Asp Val Leu Lys Asp Ser Gly Arg Asp Tyr Val Ser Gln 
            20                  25                  30          


Phe Glu Gly Ser Ala Leu Gly Lys Gln Leu Asn Leu Lys Leu Leu Asp 
        35                  40                  45              


Asn Trp Asp Ser Val Thr Ser Thr Phe Ser Lys Leu Arg Glu Gln Leu 
    50                  55                  60                  


Gly Pro Val Thr Gln Glu Phe Trp Asp Asn Leu Glu Lys Glu Thr Glu 
65                  70                  75                  80  


Gly Leu Arg Gln Glu Met Ser Lys Asp Leu Glu Glu Val Lys Ala Lys 
                85                  90                  95      


Val Gln Pro Tyr Leu Asp Asp Phe Gln Lys Lys Trp Gln Glu Glu Met 
            100                 105                 110         


Glu Leu Tyr Arg Gln Lys Val Glu Pro Leu Arg Ala Glu Leu Gln Glu 
        115                 120                 125             


Gly Ala Arg Gln Lys Leu His Glu Leu Gln Glu Lys Leu Ser Pro Leu 
    130                 135                 140                 


Gly Glu Glu Met Arg Asp Cys Ala Arg Ala His Val Asp Ala Leu Arg 
145                 150                 155                 160 


Thr His Leu Ala Pro Tyr Ser Asp Glu Leu Arg Gln Arg Leu Ala Ala 
                165                 170                 175     


Arg Leu Glu Ala Leu Lys Glu Asn Gly Gly Ala Arg Leu Ala Glu Tyr 
            180                 185                 190         


His Ala Lys Ala Thr Glu His Leu Ser Thr Leu Ser Glu Lys Ala Lys 
        195                 200                 205             


Pro Ala Leu Glu Asp Leu Arg Gln Gly Leu Leu Pro Val Leu Glu Ser 
    210                 215                 220                 


Phe Lys Val Ser Phe Leu Ser Ala Leu Glu Glu Tyr Thr Lys Lys Leu 
225                 230                 235                 240 


Asn Thr Gln 
            


<210>  4
<211>  243
<212>  PRT
<213>  Homo sapiens

<400>  4

Asp Glu Pro Pro Gln Ser Pro Trp Asp Arg Val Lys Asp Leu Ala Thr 
1               5                   10                  15      


Val Tyr Val Asp Val Leu Lys Asp Ser Gly Arg Asp Tyr Val Ser Gln 
            20                  25                  30          


Phe Glu Gly Ser Ala Leu Gly Lys Gln Leu Asn Leu Lys Leu Leu Asp 
        35                  40                  45              


Asn Trp Asp Ser Val Thr Ser Thr Phe Ser Lys Leu Arg Glu Gln Leu 
    50                  55                  60                  


Gly Pro Val Thr Gln Glu Phe Trp Asp Asn Leu Glu Lys Glu Thr Glu 
65                  70                  75                  80  


Gly Leu Arg Gln Glu Met Ser Lys Asp Leu Glu Glu Val Lys Ala Lys 
                85                  90                  95      


Val Gln Pro Tyr Leu Asp Asp Phe Gln Lys Lys Trp Gln Glu Glu Met 
            100                 105                 110         


Glu Leu Tyr Arg Gln Lys Val Glu Pro Leu Arg Ala Glu Leu Gln Glu 
        115                 120                 125             


Gly Ala Arg Gln Lys Leu His Glu Leu Gln Glu Lys Leu Ser Pro Leu 
    130                 135                 140                 


Gly Glu Glu Met Arg Asp Arg Ala Arg Ala His Val Asp Ala Leu Arg 
145                 150                 155                 160 


Thr His Leu Ala Pro Tyr Ser Asp Glu Leu Arg Gln Cys Leu Ala Ala 
                165                 170                 175     


Arg Leu Glu Ala Leu Lys Glu Asn Gly Gly Ala Arg Leu Ala Glu Tyr 
            180                 185                 190         


His Ala Lys Ala Thr Glu His Leu Ser Thr Leu Ser Glu Lys Ala Lys 
        195                 200                 205             


Pro Ala Leu Glu Asp Leu Arg Gln Gly Leu Leu Pro Val Leu Glu Ser 
    210                 215                 220                 


Phe Lys Val Ser Phe Leu Ser Ala Leu Glu Glu Tyr Thr Lys Lys Leu 
225                 230                 235                 240 


Asn Thr Gln 
            


<210>  5
<211>  792
<212>  DNA
<213>  Homo sapiens

<400>  5
atgaaaaaaa ctgctatcgc gatcgctgta gctctggctg gtttcgcgac cgtagctaac     60

gctgacgagc caccgcagag tccatgggat cgagttaaag acctggctac cgtttacgtg    120

gacgtcctga aagactctgg tcgtgactac gtgtctcagt tcgaaggtag cgctctgggt    180

aaacagctta acctcaaact gctggacaac tgggactccg tgacctccac cttcagcaaa    240

ctgcgtgaac agcttggtcc ggtgactcag gaattttggg ataacctcga aaaggaaacc    300

gagggtctgc gtcaggaaat gtctaaagac ctcgaagaag ttaaagctaa agtgcagccg    360

tacctggacg acttccaaaa aaaatggcag gaggaaatgg agctgtaccg tcagaaagtt    420

gaacctctgc gtgcggagct gcaggaaggt gcgcgtcaga aattgcacga actgcaggaa    480

aaacttagcc cgctgggtga agaaatgcgt gatcgtgctc gtgctcacgt tgatgctctg    540

cgtacccacc tggctccgta tagcgatgaa ctgcgtcaac gtttggcggc tcgtctggag    600

gctctgaaag aaaacggtgg tgcgcgtctg gctgagtacc acgctaaagc cactgaacac    660

cttagcaccc tgagcgaaaa agctaaaccg gcgcttgaag acctgcgtca gggtctgctg    720

ccggttctgg agtccttcaa agttagcttc cttagcgctc tggaggaata caccaaaaaa    780

ctgaacaccc ag                                                        792


<210>  6
<211>  264
<212>  PRT
<213>  Homo sapiens

<400>  6

Met Lys Lys Thr Ala Ile Ala Ile Ala Val Ala Leu Ala Gly Phe Ala 
1               5                   10                  15      


Thr Val Ala Asn Ala Asp Glu Pro Pro Gln Ser Pro Trp Asp Arg Val 
            20                  25                  30          


Lys Asp Leu Ala Thr Val Tyr Val Asp Val Leu Lys Asp Ser Gly Arg 
        35                  40                  45              


Asp Tyr Val Ser Gln Phe Glu Gly Ser Ala Leu Gly Lys Gln Leu Asn 
    50                  55                  60                  


Leu Lys Leu Leu Asp Asn Trp Asp Ser Val Thr Ser Thr Phe Ser Lys 
65                  70                  75                  80  


Leu Arg Glu Gln Leu Gly Pro Val Thr Gln Glu Phe Trp Asp Asn Leu 
                85                  90                  95      


Glu Lys Glu Thr Glu Gly Leu Arg Gln Glu Met Ser Lys Asp Leu Glu 
            100                 105                 110         


Glu Val Lys Ala Lys Val Gln Pro Tyr Leu Asp Asp Phe Gln Lys Lys 
        115                 120                 125             


Trp Gln Glu Glu Met Glu Leu Tyr Arg Gln Lys Val Glu Pro Leu Arg 
    130                 135                 140                 


Ala Glu Leu Gln Glu Gly Ala Arg Gln Lys Leu His Glu Leu Gln Glu 
145                 150                 155                 160 


Lys Leu Ser Pro Leu Gly Glu Glu Met Arg Asp Arg Ala Arg Ala His 
                165                 170                 175     


Val Asp Ala Leu Arg Thr His Leu Ala Pro Tyr Ser Asp Glu Leu Arg 
            180                 185                 190         


Gln Arg Leu Ala Ala Arg Leu Glu Ala Leu Lys Glu Asn Gly Gly Ala 
        195                 200                 205             


Arg Leu Ala Glu Tyr His Ala Lys Ala Thr Glu His Leu Ser Thr Leu 
    210                 215                 220                 


Ser Glu Lys Ala Lys Pro Ala Leu Glu Asp Leu Arg Gln Gly Leu Leu 
225                 230                 235                 240 


Pro Val Leu Glu Ser Phe Lys Val Ser Phe Leu Ser Ala Leu Glu Glu 
                245                 250                 255     


Tyr Thr Lys Lys Leu Asn Thr Gln 
            260                 


<210>  7
<211>  77
<212>  DNA
<213>  Methanococcus jannaschii

<400>  7
ccggcggtag ttcagcaggg cagaacggcg gactctaaat ccgcatggcg ctggttcaaa     60

tccggcccgc cggacca                                                    77


<210>  8
<211>  88
<212>  DNA
<213>  Artificial

<220>
<223>  An optimized amber supressor tRNA

<400>  8
cccagggtag ccaagctcgg ccaacggcga cggactctaa atccgttctc gtaggagttc     60

gagggttcga atcccttccc tgggacca                                        88


<210>  9
<211>  89
<212>  DNA
<213>  Artificial

<220>
<223>  An optimized AGGA frameshift supressor tRNA

<400>  9
gcgagggtag ccaagctcgg ccaacggcga cggacttcct aatccgttct cgtaggagtt     60

cgagggttcg aatccctccc ctcgcacca                                       89


<210>  10
<211>  306
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase for the incorporation of 
       p-azido-L-phenylalanine

<400>  10

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Gly 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Thr Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Thr Tyr Tyr 
145                 150                 155                 160 


Tyr Leu Gly Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile 
                165                 170                 175     


His Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His 
            180                 185                 190         


Asn Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser 
        195                 200                 205             


Lys Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala 
    210                 215                 220                 


Lys Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro 
225                 230                 235                 240 


Ile Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys 
                245                 250                 255     


Arg Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu 
            260                 265                 270         


Leu Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys 
        275                 280                 285             


Asn Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys 
    290                 295                 300                 


Arg Leu 
305     


<210>  11
<211>  306
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase  for the incorporation of 
       p-benzoyl-L-phenylalanine

<400>  11

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Gly 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Ser Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Thr Ser His 
145                 150                 155                 160 


Tyr Leu Gly Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile 
                165                 170                 175     


His Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His 
            180                 185                 190         


Asn Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser 
        195                 200                 205             


Lys Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala 
    210                 215                 220                 


Lys Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro 
225                 230                 235                 240 


Ile Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys 
                245                 250                 255     


Arg Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu 
            260                 265                 270         


Leu Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys 
        275                 280                 285             


Asn Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys 
    290                 295                 300                 


Arg Leu 
305     


<210>  12
<211>  305
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase for the incorporation of 
       propargyl-phenylalanine

<400>  12

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Ala 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Pro Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Ala Ile Tyr 
145                 150                 155                 160 


Leu Ala Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile His 
                165                 170                 175     


Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His Asn 
            180                 185                 190         


Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser Lys 
        195                 200                 205             


Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala Lys 
    210                 215                 220                 


Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro Ile 
225                 230                 235                 240 


Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys Arg 
                245                 250                 255     


Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu Leu 
            260                 265                 270         


Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys Asn 
        275                 280                 285             


Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys Arg 
    290                 295                 300                 


Leu 
305 


<210>  13
<211>  305
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase for the incorporation of 
       propargyl-phenylalanine

<400>  13

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Ala 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Pro Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Ile Pro Tyr 
145                 150                 155                 160 


Leu Pro Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile His 
                165                 170                 175     


Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His Asn 
            180                 185                 190         


Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser Lys 
        195                 200                 205             


Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala Lys 
    210                 215                 220                 


Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro Ile 
225                 230                 235                 240 


Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys Arg 
                245                 250                 255     


Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu Leu 
            260                 265                 270         


Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys Asn 
        275                 280                 285             


Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys Arg 
    290                 295                 300                 


Leu 
305 


<210>  14
<211>  305
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase for the incorporation of 
       propargyl-phenylalanine

<400>  14

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Ala 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Lys Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Ala Ile Tyr 
145                 150                 155                 160 


Leu Ala Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile His 
                165                 170                 175     


Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His Asn 
            180                 185                 190         


Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser Lys 
        195                 200                 205             


Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala Lys 
    210                 215                 220                 


Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro Ile 
225                 230                 235                 240 


Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys Arg 
                245                 250                 255     


Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu Leu 
            260                 265                 270         


Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys Asn 
        275                 280                 285             


Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys Arg 
    290                 295                 300                 


Leu 
305 


<210>  15
<211>  306
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase  for the incorporation of 
       p-azido-phenylalanine

<400>  15

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Thr 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Asn Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Pro Leu His 
145                 150                 155                 160 


Tyr Gln Gly Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile 
                165                 170                 175     


His Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His 
            180                 185                 190         


Asn Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser 
        195                 200                 205             


Lys Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala 
    210                 215                 220                 


Lys Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro 
225                 230                 235                 240 


Ile Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys 
                245                 250                 255     


Arg Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu 
            260                 265                 270         


Leu Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys 
        275                 280                 285             


Asn Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys 
    290                 295                 300                 


Arg Leu 
305     


<210>  16
<211>  306
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase  for the incorporation of 
       p-azido-phenylalanine

<400>  16

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Thr 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Ser Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Pro Leu His 
145                 150                 155                 160 


Tyr Gln Gly Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile 
                165                 170                 175     


His Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His 
            180                 185                 190         


Asn Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser 
        195                 200                 205             


Lys Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala 
    210                 215                 220                 


Lys Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro 
225                 230                 235                 240 


Ile Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys 
                245                 250                 255     


Arg Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu 
            260                 265                 270         


Leu Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys 
        275                 280                 285             


Asn Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys 
    290                 295                 300                 


Arg Leu 
305     


<210>  17
<211>  306
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase for the incorporation of 
       p-azido-phenylalanine

<400>  17

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Leu 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Thr Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Pro Val His 
145                 150                 155                 160 


Tyr Gln Gly Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile 
                165                 170                 175     


His Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His 
            180                 185                 190         


Asn Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser 
        195                 200                 205             


Lys Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala 
    210                 215                 220                 


Lys Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro 
225                 230                 235                 240 


Ile Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys 
                245                 250                 255     


Arg Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu 
            260                 265                 270         


Leu Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys 
        275                 280                 285             


Asn Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys 
    290                 295                 300                 


Arg Leu 
305     


<210>  18
<211>  306
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase  for the incorporation of 
       p-azido-phenylalanine

<400>  18

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Thr 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Ser Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Pro Ser His 
145                 150                 155                 160 


Tyr Gln Gly Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile 
                165                 170                 175     


His Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His 
            180                 185                 190         


Asn Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser 
        195                 200                 205             


Lys Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala 
    210                 215                 220                 


Lys Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro 
225                 230                 235                 240 


Ile Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys 
                245                 250                 255     


Arg Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu 
            260                 265                 270         


Leu Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys 
        275                 280                 285             


Asn Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys 
    290                 295                 300                 


Arg Leu 
305     


<210>  19
<211>  306
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase  for the incorporation of 
       p-acetyl-phenylalanine

<400>  19

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Leu 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Glu Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Gly Cys His 
145                 150                 155                 160 


Tyr Arg Gly Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile 
                165                 170                 175     


His Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His 
            180                 185                 190         


Asn Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser 
        195                 200                 205             


Lys Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala 
    210                 215                 220                 


Lys Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro 
225                 230                 235                 240 


Ile Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys 
                245                 250                 255     


Arg Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu 
            260                 265                 270         


Leu Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys 
        275                 280                 285             


Asn Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys 
    290                 295                 300                 


Arg Leu 
305     


<210>  20
<211>  306
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase  for the incorporation of p-acetyl 
       -phenylalanine

<400>  20

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Leu 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Glu Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Gly Thr His 
145                 150                 155                 160 


Tyr Arg Gly Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile 
                165                 170                 175     


His Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His 
            180                 185                 190         


Asn Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser 
        195                 200                 205             


Lys Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala 
    210                 215                 220                 


Lys Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro 
225                 230                 235                 240 


Ile Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys 
                245                 250                 255     


Arg Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu 
            260                 265                 270         


Leu Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys 
        275                 280                 285             


Asn Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys 
    290                 295                 300                 


Arg Leu 
305     


<210>  21
<211>  306
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase  for the incorporation of 
       p-acetyl-phenylalanine

<400>  21

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Ala 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Glu Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Gly Gly His 
145                 150                 155                 160 


Tyr Leu Gly Val Asp Val Ile Val Gly Gly Met Glu Gln Arg Lys Ile 
                165                 170                 175     


His Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His 
            180                 185                 190         


Asn Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser 
        195                 200                 205             


Lys Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala 
    210                 215                 220                 


Lys Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro 
225                 230                 235                 240 


Ile Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys 
                245                 250                 255     


Arg Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu 
            260                 265                 270         


Leu Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys 
        275                 280                 285             


Asn Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys 
    290                 295                 300                 


Arg Leu 
305     


<210>  22
<211>  306
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase  for the incorporation of 
       p-azido-phenylalanine

<400>  22

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Ala 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Arg Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Val Ile His 
145                 150                 155                 160 


Tyr Asp Gly Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile 
                165                 170                 175     


His Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His 
            180                 185                 190         


Asn Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser 
        195                 200                 205             


Lys Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala 
    210                 215                 220                 


Lys Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro 
225                 230                 235                 240 


Ile Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys 
                245                 250                 255     


Arg Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu 
            260                 265                 270         


Leu Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys 
        275                 280                 285             


Asn Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys 
    290                 295                 300                 


Arg Leu 
305     


<210>  23
<211>  306
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase  for the incorporation of 
       p-azido-phenylalanine

<400>  23

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Gly 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Thr Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Thr Tyr Tyr 
145                 150                 155                 160 


Tyr Leu Gly Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile 
                165                 170                 175     


His Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His 
            180                 185                 190         


Asn Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser 
        195                 200                 205             


Lys Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala 
    210                 215                 220                 


Lys Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro 
225                 230                 235                 240 


Ile Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys 
                245                 250                 255     


Arg Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu 
            260                 265                 270         


Leu Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys 
        275                 280                 285             


Asn Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys 
    290                 295                 300                 


Arg Leu 
305     


<210>  24
<211>  306
<212>  PRT
<213>  Artificial

<220>
<223>  ADD

<400>  24

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Val 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Tyr Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Glu His Gly Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Gly Leu His 
145                 150                 155                 160 


Tyr Glu Gly Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile 
                165                 170                 175     


His Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His 
            180                 185                 190         


Asn Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser 
        195                 200                 205             


Lys Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala 
    210                 215                 220                 


Lys Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro 
225                 230                 235                 240 


Ile Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys 
                245                 250                 255     


Arg Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu 
            260                 265                 270         


Leu Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys 
        275                 280                 285             


Asn Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys 
    290                 295                 300                 


Arg Leu 
305     


<210>  25
<211>  306
<212>  PRT
<213>  Artificial

<220>
<223>  Mutant synthetase derived from Methanococcus jannaschii 
       synthetase

<400>  25

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Val 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Tyr Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Glu His Gly Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Gly Ile His 
145                 150                 155                 160 


Tyr Glu Gly Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile 
                165                 170                 175     


His Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His 
            180                 185                 190         


Asn Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser 
        195                 200                 205             


Lys Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala 
    210                 215                 220                 


Lys Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro 
225                 230                 235                 240 


Ile Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys 
                245                 250                 255     


Arg Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu 
            260                 265                 270         


Leu Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Arg Leu Lys 
        275                 280                 285             


Asn Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys 
    290                 295                 300                 


Arg Leu 
305     


<210>  26
<211>  88
<212>  DNA
<213>  Halobacterium sp. NRC-1

<400>  26
cccagggtag ccaagctcgg ccaacggcga cggactctaa atccgttctc gtaggagttc     60

gagggttcga atcccttccc tgggacca                                        88


