
                                SEQUENCE LISTING

<110> Allozyne, Ince
       Grabstein, Kenneth H.    
      Wang, Aijun
      Winblade, Natalie
	Graddis, Thomas James
	McCraith, Stephen
	Datta, Deepshikha

   
<120> AMINO ACID SUBSTITUTED MOLECULES
  
<130> 110197.41001PC

<140> PCT                  
<141> 2008-08-20

<150> US 12/105,155
<151> 2008-04-17

<150> US 12/105,154
<151> 2008-04-17

<150> US 11/743,608
<151> 2007-05-02


<160> 18

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 564
<212> DNA
<213> Homo sapiens

<400> 1
atgaccaaca agtgtctcct ccaaattgct ctcctgttgt gcttctccac tacagctctt 60
tccatgagct acaacttgct tggattccta caaagaagca gcaattttca gtgtcagaag 120
ctcctgtggc aattgaatgg gaggcttgaa tactgcctca aggacaggat gaactttgac 180
atccctgagg agattaagca gctgcagcag ttccagaagg aggacgccgc attgaccatc 240
tatgagatgc tccagaacat ctttgctatt ttcagacaag attcatctag cactggctgg 300
aatgagacta ttgttgagaa cctcctggct aatgtctatc atcagataaa ccatctgaag 360
acagtcctgg aagaaaaact ggagaaagaa gatttcacca ggggaaaact catgagcagt 420
ctgcacctga aaagatatta tgggaggatt ctgcattacc tgaaggccaa ggagtacagt 480
cactgtgcct ggaccatagt cagagtggaa atcctaagga acttttactt cattaacaga 540
cttacaggtt acctccgaaa ctga                                        564

<210> 2
<211> 187
<212> PRT
<213> Homo sapiens

<400> 2
Met Thr Asn Lys Cys Leu Leu Gln Ile Ala Leu Leu Leu Cys Phe Ser
 1               5                  10                  15      
Thr Thr Ala Leu Ser Met Ser Tyr Asn Leu Leu Gly Phe Leu Gln Arg
            20                  25                  30          
Ser Ser Asn Phe Gln Cys Gln Lys Leu Leu Trp Gln Leu Asn Gly Arg
        35                  40                  45              
Leu Glu Tyr Cys Leu Lys Asp Arg Met Asn Phe Asp Ile Pro Glu Glu
    50                  55                  60                  
Ile Lys Gln Leu Gln Gln Phe Gln Lys Glu Asp Ala Ala Leu Thr Ile
65                  70                  75                  80  
Tyr Glu Met Leu Gln Asn Ile Phe Ala Ile Phe Arg Gln Asp Ser Ser
                85                  90                  95      
Ser Thr Gly Trp Asn Glu Thr Ile Val Glu Asn Leu Leu Ala Asn Val
            100                 105                 110         
Tyr His Gln Ile Asn His Leu Lys Thr Val Leu Glu Glu Lys Leu Glu
        115                 120                 125             
Lys Glu Asp Phe Thr Arg Gly Lys Leu Met Ser Ser Leu His Leu Lys
    130                 135                 140                 
Arg Tyr Tyr Gly Arg Ile Leu His Tyr Leu Lys Ala Lys Glu Tyr Ser
145                 150                 155                 160 
His Cys Ala Trp Thr Ile Val Arg Val Glu Ile Leu Arg Asn Phe Tyr
                165                 170                 175     
Phe Ile Asn Arg Leu Thr Gly Tyr Leu Arg Asn
            180                 185         


<210> 3
<211> 501
<212> DNA
<213> Artificial Sequence

<220> 
<223> Modified human interferon (Synthetic sequence IFNb
      C17S)

<400> 3
atgagctata atctgttagg ctttctgcaa cgtagcagca attttcagag ccagaaattg 60
ttatggcaac tgaatggtcg tcttgaatac tgtttgaagg accgcatgaa ctttgacatc 120
ccggaagaaa ttaagcaact gcaacagttc cagaaagaag acgccgcact gaccatctat 180
gagatgctcc agaacatctt tgctattttc cgtcaagatt catctagcac tggctggaat 240
gagactattg ttgagaattt actggcgaat gtctatcatc agatcaacca tctgaaaacc 300
gtgctggaag aaaaactgga gaaagaagat ttcacccgtg gtaaactgat gagcagtctg 360
cacctgaaac gctattatgg ccgtattctg cattacctga aggcaaaaga atacagtcac 420
tgtgcctgga ccatcgtccg tgtggaaatc ctgcgcaact tttacttcat taaccgtctt 480
acgggttacc tgcgcaacta a                                           501

<210> 4
<211> 166
<212> PRT
<213> Artificial Sequence

<220> 
<223> Modified human interferon (Synthetic sequence IFNb
      C17S)

<400> 4
Met Ser Tyr Asn Leu Leu Gly Phe Leu Gln Arg Ser Ser Asn Phe Gln
 1               5                  10                  15      
Ser Gln Lys Leu Leu Trp Gln Leu Asn Gly Arg Leu Glu Tyr Cys Leu
            20                  25                  30          
Lys Asp Arg Met Asn Phe Asp Ile Pro Glu Glu Ile Lys Gln Leu Gln
        35                  40                  45              
Gln Phe Gln Lys Glu Asp Ala Ala Leu Thr Ile Tyr Glu Met Leu Gln
    50                  55                  60                  
Asn Ile Phe Ala Ile Phe Arg Gln Asp Ser Ser Ser Thr Gly Trp Asn
65                  70                  75                  80  
Glu Thr Ile Val Glu Asn Leu Leu Ala Asn Val Tyr His Gln Ile Asn
                85                  90                  95      
His Leu Lys Thr Val Leu Glu Glu Lys Leu Glu Lys Glu Asp Phe Thr
            100                 105                 110         
Arg Gly Lys Leu Met Ser Ser Leu His Leu Lys Arg Tyr Tyr Gly Arg
        115                 120                 125             
Ile Leu His Tyr Leu Lys Ala Lys Glu Tyr Ser His Cys Ala Trp Thr
    130                 135                 140                 
Ile Val Arg Val Glu Ile Leu Arg Asn Phe Tyr Phe Ile Asn Arg Leu
145                 150                 155                 160 
Thr Gly Tyr Leu Arg Asn
                165     


<210> 5
<211> 501
<212> DNA
<213> Artificial Sequence

<220> 
<223> Modified human interferon (Synthetic sequence IFNb
      TIS2E triple C17S)

<400> 5
atggagtata atctgttagg ctttctgcaa cgtagcagca attttcagag ccagaaattg 60
ttatggcaac tgaatggtcg tcttgaatac tgtttgaagg accgcattaa ctttgacttc 120
ccggaagaac tgaagcaact gcaacagttc cagaaagaag acgccgcact gaccatctat 180
gagatcctcc agaacatctt tgctattttc cgtcaagatt catctagcac tggctggaat 240
gagactattg ttgagaattt actggcgaat gtctatcatc agatcaacca tctgaaaacc 300
gtgctggaag aaaaactgga gaaagaagat ttcacccgtg gtaaactgac gagcagtctg 360
cacctgaaac gctattatgg ccgtattctg cattacctga aggcaaaaga atacagtcac 420
tgtgcctgga ccatcgtccg tgtggaaatc ctgcgcaact tttacttcat taaccgtctt 480
acgggttacc tgcgcaacta a                                           501

<210> 6
<211> 166
<212> PRT
<213> Artificial Sequence

<220> 
<223> Modified human interferon (Synthetic sequence IFNb
      TIS2E triple C17S)

<400> 6
Met Glu Tyr Asn Leu Leu Gly Phe Leu Gln Arg Ser Ser Asn Phe Gln
 1               5                  10                  15      
Ser Gln Lys Leu Leu Trp Gln Leu Asn Gly Arg Leu Glu Tyr Cys Leu
            20                  25                  30          
Lys Asp Arg Ile Asn Phe Asp Phe Pro Glu Glu Leu Lys Gln Leu Gln
        35                  40                  45              
Gln Phe Gln Lys Glu Asp Ala Ala Leu Thr Ile Tyr Glu Ile Leu Gln
    50                  55                  60                  
Asn Ile Phe Ala Ile Phe Arg Gln Asp Ser Ser Ser Thr Gly Trp Asn
65                  70                  75                  80  
Glu Thr Ile Val Glu Asn Leu Leu Ala Asn Val Tyr His Gln Ile Asn
                85                  90                  95      
His Leu Lys Thr Val Leu Glu Glu Lys Leu Glu Lys Glu Asp Phe Thr
            100                 105                 110         
Arg Gly Lys Leu Thr Ser Ser Leu His Leu Lys Arg Tyr Tyr Gly Arg
        115                 120                 125             
Ile Leu His Tyr Leu Lys Ala Lys Glu Tyr Ser His Cys Ala Trp Thr
    130                 135                 140                 
Ile Val Arg Val Glu Ile Leu Arg Asn Phe Tyr Phe Ile Asn Arg Leu
145                 150                 155                 160 
Thr Gly Tyr Leu Arg Asn
                165     


<210> 7
<211> 33
<212> DNA
<213> Artificial Sequence

<220> 
<223> Oligonucleotide sequence encoding N-terminal
      tryptic peptide that was introduced in the mutein.

<400> 7
atggcgtata atctgttagg ctttctgcaa cgt                              33

<210> 8
<211> 11
<212> PRT
<213> Artificial Sequence

<220> 
<223> Penultimate amino acid residue introduced to the
      mutein.

<220>
<221> VARIANT        
<222> 1
<223> Xaa = Any Amino Acid

<400> 8
Xaa Gln Tyr Asn Leu Leu Gly Phe Leu Gln Arg
 1               5                  10      


<210> 9
<211> 33
<212> DNA
<213> Artificial Sequence

<220> 
<223> Oligonucleotide sequence encoding N-terminal
      tryptic peptide that was introduced in the mutein.

<400> 9
atgagctata atctgttagg ctttctgcaa cgt                              33

<210> 10
<211> 11
<212> PRT
<213> Artificial Sequence

<220> 
<223> Penultimate amino acid residue introduced to the
      mutein.

<220>
<221> VARIANT        
<222> 1
<223> Xaa = Any Amino Acid

<400> 10
Xaa Ser Tyr Asn Leu Leu Gly Phe Leu Gln Arg
 1               5                  10      


<210> 11
<211> 33
<212> DNA
<213> Artificial Sequence

<220> 
<223> Oligonucleotide sequence encoding N-terminal
      tryptic peptide that was introduced in the mutein.

<400> 11
atgggctata atctgttagg ctttctgcaa cgt                              33

<210> 12
<211> 11
<212> PRT
<213> Artificial Sequence

<220> 
<223> Penultimate amino acid residue introduced to the
      mutein.

<220>
<221> VARIANT        
<222> 1
<223> Xaa = Any Amino Acid

<400> 12
Xaa Gly Tyr Asn Leu Leu Gly Phe Leu Gln Arg
 1               5                  10      


<210> 13
<211> 33
<212> DNA
<213> Artificial Sequence

<220> 
<223> Oligonucleotide sequence encoding N-terminal
      tryptic peptide that was introduced in the mutein.

<400> 13
atgcactata atctgttagg ctttctgcaa cgt                              33

<210> 14
<211> 11
<212> PRT
<213> Artificial Sequence

<220> 
<223> Penultimate amino acid residue introduced to the
      mutein.

<220>
<221> VARIANT        
<222> 1
<223> Xaa = Any Amino Acid

<400> 14
Xaa His Tyr Asn Leu Leu Gly Phe Leu Gln Arg
 1               5                  10      


<210> 15
<211> 33
<212> DNA
<213> Artificial Sequence

<220> 
<223> Oligonucleotide sequence encoding N-terminal
      tryptic peptide that was introduced in the mutein.

<400> 15
atgcagtata atctgttagg ctttctgcaa cgt                              33

<210> 16
<211> 11
<212> PRT
<213> Artificial Sequence

<220> 
<223> Penultimate amino acid residue introduced to the
      mutein.

<220>
<221> VARIANT        
<222> 1
<223> Xaa = Any Amino Acid

<400> 16
Xaa Gln Tyr Asn Leu Leu Gly Phe Leu Gln Arg
 1               5                  10      


<210> 17
<211> 33
<212> DNA
<213> Artificial Sequence

<220> 
<223> Oligonucleotide sequence encoding N-terminal
      tryptic peptide that was introduced in the mutein.

<400> 17
atggagtata atctgttagg ctttctgcaa cgt                              33

<210> 18
<211> 11
<212> PRT
<213> Artificial Sequence

<220> 
<223> Penultimate amino acid residue introduced to the
      mutein.

<220>
<221> VARIANT        
<222> 1
<223> Xaa = Any Amino Acid

<400> 18
Xaa Glu Tyr Asn Leu Leu Gly Phe Leu Gln Arg
 1               5                  10      






