                         SEQUENCE LISTING

<110>  University of Georgia Research Foundation, Inc.
 
<120>  Enhancement of Bacillus Thuringiensis CRY Protein Toxicities to 
       Coleopterans, and Novel Insect Cadherin Fragments

<130>  UGR-109XC1

<150>  US 60/964,249
<151>  2007-08-10

<150>  US 61/084,952
<151>  2008-07-30

<160>  12    

<170>  PatentIn version 3.3

<210>  1
<211>  960
<212>  DNA
<213>  Manduca sexta


<220>
<221>  CDS
<222>  (1)..(957)

<400>  1
atg cac ttg gag cgg ata tcg gcg act gac ccg gac gga ctc cac gcg       48
Met His Leu Glu Arg Ile Ser Ala Thr Asp Pro Asp Gly Leu His Ala         
1               5                   10                  15              

ggc gtc gtc acc ttc caa gtg gta ggc gat gag gaa tca caa cgg tac       96
Gly Val Val Thr Phe Gln Val Val Gly Asp Glu Glu Ser Gln Arg Tyr         
            20                  25                  30                  

ttt caa gta gtt aac gat ggc gcg aac ctc ggc tcg ttg agg tta ctg      144
Phe Gln Val Val Asn Asp Gly Ala Asn Leu Gly Ser Leu Arg Leu Leu         
        35                  40                  45                      

caa gcc gtt cca gag gag atc agg gag ttc cgg ata acg att cgc gct      192
Gln Ala Val Pro Glu Glu Ile Arg Glu Phe Arg Ile Thr Ile Arg Ala         
    50                  55                  60                          

aca gac cag gga acg gac cca gga ccg ctg tcc acg gac atg acg ttc      240
Thr Asp Gln Gly Thr Asp Pro Gly Pro Leu Ser Thr Asp Met Thr Phe         
65                  70                  75                  80          

aga gtt gtt ttt gtg ccc acg caa gga gaa cct aga ttc gcg tcc tca      288
Arg Val Val Phe Val Pro Thr Gln Gly Glu Pro Arg Phe Ala Ser Ser         
                85                  90                  95              

gaa cat gct gtc gct ttc ata gaa aag agt gcc ggc atg gaa gag tct      336
Glu His Ala Val Ala Phe Ile Glu Lys Ser Ala Gly Met Glu Glu Ser         
            100                 105                 110                 

cac caa ctt cct cta gca caa gac atc aag aac cat ctc tgt gaa gac      384
His Gln Leu Pro Leu Ala Gln Asp Ile Lys Asn His Leu Cys Glu Asp         
        115                 120                 125                     

gac tgt cac agc att tac tat cgt att atc gat ggc aac agc gag ggt      432
Asp Cys His Ser Ile Tyr Tyr Arg Ile Ile Asp Gly Asn Ser Glu Gly         
    130                 135                 140                         

cat ttc ggc ctg gat cct gtt cgc aac agg ttg ttc ctg aag aaa gag      480
His Phe Gly Leu Asp Pro Val Arg Asn Arg Leu Phe Leu Lys Lys Glu         
145                 150                 155                 160         

ctg ata aga gaa caa agt gcc tcc cac act ctg caa gtg gcg gct agt      528
Leu Ile Arg Glu Gln Ser Ala Ser His Thr Leu Gln Val Ala Ala Ser         
                165                 170                 175             

aac tcg ccc gat ggt ggc att cca ctt cct gct tcc atc ctt act gtc      576
Asn Ser Pro Asp Gly Gly Ile Pro Leu Pro Ala Ser Ile Leu Thr Val         
            180                 185                 190                 

act gtt acc gtg agg gag gca gac cct cgt cca gtg ttt atg agg gaa      624
Thr Val Thr Val Arg Glu Ala Asp Pro Arg Pro Val Phe Met Arg Glu         
        195                 200                 205                     

ttg tac acc gca ggg ata tcc aca gcg gac tcc atc ggc aga gag ctg      672
Leu Tyr Thr Ala Gly Ile Ser Thr Ala Asp Ser Ile Gly Arg Glu Leu         
    210                 215                 220                         

ctc aga tta cat gcg acc cag tct gaa ggc gcg gcc att act tat gct      720
Leu Arg Leu His Ala Thr Gln Ser Glu Gly Ala Ala Ile Thr Tyr Ala         
225                 230                 235                 240         

ata gac tac gat aca atg gta gtg gac ccc agc ctg gag gca gtg aga      768
Ile Asp Tyr Asp Thr Met Val Val Asp Pro Ser Leu Glu Ala Val Arg         
                245                 250                 255             

cag tcg gct ttc gta ctg aac gct caa acc gga gtg ctg acg ctt aat      816
Gln Ser Ala Phe Val Leu Asn Ala Gln Thr Gly Val Leu Thr Leu Asn         
            260                 265                 270                 

atc cag ccc acg gcc acg atg cat gga ctg ttc aaa ttc gaa gtc aca      864
Ile Gln Pro Thr Ala Thr Met His Gly Leu Phe Lys Phe Glu Val Thr         
        275                 280                 285                     

gct act gac acg gcc ggc gct cag gac cgc acc gac gtc acc gtg tac      912
Ala Thr Asp Thr Ala Gly Ala Gln Asp Arg Thr Asp Val Thr Val Tyr         
    290                 295                 300                         

gtg gta tcc tcg cag aac cgc ctc gag cac cac cac cac cac cac tga      960
Val Val Ser Ser Gln Asn Arg Leu Glu His His His His His His             
305                 310                 315                             


<210>  2
<211>  319
<212>  PRT
<213>  Manduca sexta

<400>  2

Met His Leu Glu Arg Ile Ser Ala Thr Asp Pro Asp Gly Leu His Ala 
1               5                   10                  15      


Gly Val Val Thr Phe Gln Val Val Gly Asp Glu Glu Ser Gln Arg Tyr 
            20                  25                  30          


Phe Gln Val Val Asn Asp Gly Ala Asn Leu Gly Ser Leu Arg Leu Leu 
        35                  40                  45              


Gln Ala Val Pro Glu Glu Ile Arg Glu Phe Arg Ile Thr Ile Arg Ala 
    50                  55                  60                  


Thr Asp Gln Gly Thr Asp Pro Gly Pro Leu Ser Thr Asp Met Thr Phe 
65                  70                  75                  80  


Arg Val Val Phe Val Pro Thr Gln Gly Glu Pro Arg Phe Ala Ser Ser 
                85                  90                  95      


Glu His Ala Val Ala Phe Ile Glu Lys Ser Ala Gly Met Glu Glu Ser 
            100                 105                 110         


His Gln Leu Pro Leu Ala Gln Asp Ile Lys Asn His Leu Cys Glu Asp 
        115                 120                 125             


Asp Cys His Ser Ile Tyr Tyr Arg Ile Ile Asp Gly Asn Ser Glu Gly 
    130                 135                 140                 


His Phe Gly Leu Asp Pro Val Arg Asn Arg Leu Phe Leu Lys Lys Glu 
145                 150                 155                 160 


Leu Ile Arg Glu Gln Ser Ala Ser His Thr Leu Gln Val Ala Ala Ser 
                165                 170                 175     


Asn Ser Pro Asp Gly Gly Ile Pro Leu Pro Ala Ser Ile Leu Thr Val 
            180                 185                 190         


Thr Val Thr Val Arg Glu Ala Asp Pro Arg Pro Val Phe Met Arg Glu 
        195                 200                 205             


Leu Tyr Thr Ala Gly Ile Ser Thr Ala Asp Ser Ile Gly Arg Glu Leu 
    210                 215                 220                 


Leu Arg Leu His Ala Thr Gln Ser Glu Gly Ala Ala Ile Thr Tyr Ala 
225                 230                 235                 240 


Ile Asp Tyr Asp Thr Met Val Val Asp Pro Ser Leu Glu Ala Val Arg 
                245                 250                 255     


Gln Ser Ala Phe Val Leu Asn Ala Gln Thr Gly Val Leu Thr Leu Asn 
            260                 265                 270         


Ile Gln Pro Thr Ala Thr Met His Gly Leu Phe Lys Phe Glu Val Thr 
        275                 280                 285             


Ala Thr Asp Thr Ala Gly Ala Gln Asp Arg Thr Asp Val Thr Val Tyr 
    290                 295                 300                 


Val Val Ser Ser Gln Asn Arg Leu Glu His His His His His His 
305                 310                 315                 


<210>  3
<211>  960
<212>  DNA
<213>  Artificial sequence

<220>
<223>  protease stabilized version of SEQ ID NOs: 1 and 2


<220>
<221>  CDS
<222>  (1)..(957)

<400>  3
atg cac ctg gaa tgt atc tct gca acc gac ccg gat ggc ctg cat gct       48
Met His Leu Glu Cys Ile Ser Ala Thr Asp Pro Asp Gly Leu His Ala         
1               5                   10                  15              

ggt gta gta act ttc caa gtg gtt ggt gac gaa gaa agc cag gct tat       96
Gly Val Val Thr Phe Gln Val Val Gly Asp Glu Glu Ser Gln Ala Tyr         
            20                  25                  30                  

ttc cag gtt gtt aac gac ggt gca aac ctg ggc tcc ctt tcc ctg ctg      144
Phe Gln Val Val Asn Asp Gly Ala Asn Leu Gly Ser Leu Ser Leu Leu         
        35                  40                  45                      

cag gcc gtg cca gaa gaa atc gca gag ttc agc att acc atc tgc gct      192
Gln Ala Val Pro Glu Glu Ile Ala Glu Phe Ser Ile Thr Ile Cys Ala         
    50                  55                  60                          

acc gac caa ggt acc gac ccg ggc ccg ctg agc acc gac atg acc ttc      240
Thr Asp Gln Gly Thr Asp Pro Gly Pro Leu Ser Thr Asp Met Thr Phe         
65                  70                  75                  80          

gct gtt gta ttc gtt cct act cag ggt gaa cca gct ttc gct tcc tct      288
Ala Val Val Phe Val Pro Thr Gln Gly Glu Pro Ala Phe Ala Ser Ser         
                85                  90                  95              

gag cac gca gta gca ttc atc gaa gcc tcc gcg ggt atg gaa gaa tct      336
Glu His Ala Val Ala Phe Ile Glu Ala Ser Ala Gly Met Glu Glu Ser         
            100                 105                 110                 

cat cag ctc cca ctg gct caa gat atc gcg aac cat ctg tgt gaa gac      384
His Gln Leu Pro Leu Ala Gln Asp Ile Ala Asn His Leu Cys Glu Asp         
        115                 120                 125                     

gac tgc cac tct atc tac tac gct atc atc gac ggt aac agc gaa ggt      432
Asp Cys His Ser Ile Tyr Tyr Ala Ile Ile Asp Gly Asn Ser Glu Gly         
    130                 135                 140                         

cac ttc ggt ctg gac ccg gta gct aac gcg ctg ttc ctg tct gct gaa      480
His Phe Gly Leu Asp Pro Val Ala Asn Ala Leu Phe Leu Ser Ala Glu         
145                 150                 155                 160         

ctg atc gcg gaa cag agc gct tct cac act tta caa gtt gct gcg tcc      528
Leu Ile Ala Glu Gln Ser Ala Ser His Thr Leu Gln Val Ala Ala Ser         
                165                 170                 175             

aac agc ccg gac ggt ggc atc cct ctg cct gca tct atc ctt acc gtt      576
Asn Ser Pro Asp Gly Gly Ile Pro Leu Pro Ala Ser Ile Leu Thr Val         
            180                 185                 190                 

acc gta acc gtc gct gaa gca gat cca gca ccg gta ttc atg gct gag      624
Thr Val Thr Val Ala Glu Ala Asp Pro Ala Pro Val Phe Met Ala Glu         
        195                 200                 205                     

ctg tac acg gct ggc atc agc act gcc gac tcc att ggc tgc gaa ctt      672
Leu Tyr Thr Ala Gly Ile Ser Thr Ala Asp Ser Ile Gly Cys Glu Leu         
    210                 215                 220                         

ctg gct ctg cat gcg act cag tca gaa ggc gcg gcc atc acc tat gct      720
Leu Ala Leu His Ala Thr Gln Ser Glu Gly Ala Ala Ile Thr Tyr Ala         
225                 230                 235                 240         

atc gac tat gat acc atg gta gtt gat ccg tct ctg gaa gca gtt tgc      768
Ile Asp Tyr Asp Thr Met Val Val Asp Pro Ser Leu Glu Ala Val Cys         
                245                 250                 255             

cag tct gct ttc gtt ctg aac gca cag act ggt gtt ctg act ctg aac      816
Gln Ser Ala Phe Val Leu Asn Ala Gln Thr Gly Val Leu Thr Leu Asn         
            260                 265                 270                 

atc cag ccg act gca acg atg cat ggt ctg ttc aac ttc gaa gtt act      864
Ile Gln Pro Thr Ala Thr Met His Gly Leu Phe Asn Phe Glu Val Thr         
        275                 280                 285                     

gcg acc gac act gcg ggc gct cag gac cgt act gac gtt acc gtc tac      912
Ala Thr Asp Thr Ala Gly Ala Gln Asp Arg Thr Asp Val Thr Val Tyr         
    290                 295                 300                         

gta gtg tct tct cag aac cgt ctg gaa cac cac cac cac cac cac taa      960
Val Val Ser Ser Gln Asn Arg Leu Glu His His His His His His             
305                 310                 315                             


<210>  4
<211>  319
<212>  PRT
<213>  Artificial sequence

<220>
<223>  protease stabilized version of SEQ ID NOs: 1 and 2

<400>  4

Met His Leu Glu Cys Ile Ser Ala Thr Asp Pro Asp Gly Leu His Ala 
1               5                   10                  15      


Gly Val Val Thr Phe Gln Val Val Gly Asp Glu Glu Ser Gln Ala Tyr 
            20                  25                  30          


Phe Gln Val Val Asn Asp Gly Ala Asn Leu Gly Ser Leu Ser Leu Leu 
        35                  40                  45              


Gln Ala Val Pro Glu Glu Ile Ala Glu Phe Ser Ile Thr Ile Cys Ala 
    50                  55                  60                  


Thr Asp Gln Gly Thr Asp Pro Gly Pro Leu Ser Thr Asp Met Thr Phe 
65                  70                  75                  80  


Ala Val Val Phe Val Pro Thr Gln Gly Glu Pro Ala Phe Ala Ser Ser 
                85                  90                  95      


Glu His Ala Val Ala Phe Ile Glu Ala Ser Ala Gly Met Glu Glu Ser 
            100                 105                 110         


His Gln Leu Pro Leu Ala Gln Asp Ile Ala Asn His Leu Cys Glu Asp 
        115                 120                 125             


Asp Cys His Ser Ile Tyr Tyr Ala Ile Ile Asp Gly Asn Ser Glu Gly 
    130                 135                 140                 


His Phe Gly Leu Asp Pro Val Ala Asn Ala Leu Phe Leu Ser Ala Glu 
145                 150                 155                 160 


Leu Ile Ala Glu Gln Ser Ala Ser His Thr Leu Gln Val Ala Ala Ser 
                165                 170                 175     


Asn Ser Pro Asp Gly Gly Ile Pro Leu Pro Ala Ser Ile Leu Thr Val 
            180                 185                 190         


Thr Val Thr Val Ala Glu Ala Asp Pro Ala Pro Val Phe Met Ala Glu 
        195                 200                 205             


Leu Tyr Thr Ala Gly Ile Ser Thr Ala Asp Ser Ile Gly Cys Glu Leu 
    210                 215                 220                 


Leu Ala Leu His Ala Thr Gln Ser Glu Gly Ala Ala Ile Thr Tyr Ala 
225                 230                 235                 240 


Ile Asp Tyr Asp Thr Met Val Val Asp Pro Ser Leu Glu Ala Val Cys 
                245                 250                 255     


Gln Ser Ala Phe Val Leu Asn Ala Gln Thr Gly Val Leu Thr Leu Asn 
            260                 265                 270         


Ile Gln Pro Thr Ala Thr Met His Gly Leu Phe Asn Phe Glu Val Thr 
        275                 280                 285             


Ala Thr Asp Thr Ala Gly Ala Gln Asp Arg Thr Asp Val Thr Val Tyr 
    290                 295                 300                 


Val Val Ser Ser Gln Asn Arg Leu Glu His His His His His His 
305                 310                 315                 


<210>  5
<211>  1160
<212>  DNA
<213>  Artificial sequence

<220>
<223>  version of SEQ ID NO: 6 with cloning sites

<400>  5
gaattcaagg agatatacat atggctgttc ttatgactaa agacctgcag tgctctgaaa     60

acctgaacaa ggatggtgaa gtaggtgagg gcatcattgg tgaagacatt gatgatggtg    120

acaacgctaa aattgacttc tctgttttgt ctattgtaga taaggaaact aagaacgaca    180

tccaggaatc ctttaacatc tccaaaattg attctgatta tgtgctcaac gacactctga    240

agaaagtaca cctgattgct tttgaagatc tgaaaggtaa atatggaacc tatgaagtaa    300

ccctccacat gcatgatgaa ggtgacccaa tgcagactac tgacccagat ccaaccctga    360

ccctgaccat tgagaaatgg aactaccaga cccctagcat tattttccca gagaacgacc    420

agacctacat tgtgctgagc gaccagcagc ctggtcagcc actggcactg tttaacaaca    480

ctggtacatc caacactctg ccagacttct cagctactga tggtgagact aaagactata    540

gcaaatggga tgtaaagttc agctacaccc agaccaacta tgaagatgac aagatctttg    600

ttattgatca catccagcca tgcgtttccc agcttcaggt tagcaaacac ttcaactctg    660

acctggttcg ctccaagaaa tacaaactga ctatcactgc ttctgtgaag gatggtgctg    720

aacaggaagg tgaggctggc tactctactt ctgcaaacat ctctattgtt ttcctgaaca    780

acgatgctca gccaatcttt cagaactctg actggtctgt ttcctttgtt gagttcaaca    840

ccactcagcc agcaaaacca ctggaagaac aggcagaata tgaaaacacc aagggtggcc    900

tgccgatcta ttaccatttc tactctgaaa accagaccct gtccaaatac tttgaggttg    960

atgagacttc tggtgacctg tctgttattg gtaacctgac ttatgactat gaccaggaca   1020

tcagctttca cattgttgct tccaacgatt ctcaggtacg catgctggac ccacgctctt   1080

ctctgaacgt aactgtaaac ttcctgccac gtaaccgtcg tgccccacag tggcaccacc   1140

accaccacca ctaaaagctt                                               1160


<210>  6
<211>  1134
<212>  DNA
<213>  Diabrotica virgifera


<220>
<221>  CDS
<222>  (1)..(1131)

<400>  6
atg gct gtt ctt atg act aaa gac ctg cag tgc tct gaa aac ctg aac       48
Met Ala Val Leu Met Thr Lys Asp Leu Gln Cys Ser Glu Asn Leu Asn         
1               5                   10                  15              

aag gat ggt gaa gta ggt gag ggc atc att ggt gaa gac att gat gat       96
Lys Asp Gly Glu Val Gly Glu Gly Ile Ile Gly Glu Asp Ile Asp Asp         
            20                  25                  30                  

ggt gac aac gct aaa att gac ttc tct gtt ttg tct att gta gat aag      144
Gly Asp Asn Ala Lys Ile Asp Phe Ser Val Leu Ser Ile Val Asp Lys         
        35                  40                  45                      

gaa act aag aac gac atc cag gaa tcc ttt aac atc tcc aaa att gat      192
Glu Thr Lys Asn Asp Ile Gln Glu Ser Phe Asn Ile Ser Lys Ile Asp         
    50                  55                  60                          

tct gat tat gtg ctc aac gac act ctg aag aaa gta cac ctg att gct      240
Ser Asp Tyr Val Leu Asn Asp Thr Leu Lys Lys Val His Leu Ile Ala         
65                  70                  75                  80          

ttt gaa gat ctg aaa ggt aaa tat gga acc tat gaa gta acc ctc cac      288
Phe Glu Asp Leu Lys Gly Lys Tyr Gly Thr Tyr Glu Val Thr Leu His         
                85                  90                  95              

atg cat gat gaa ggt gac cca atg cag act act gac cca gat cca acc      336
Met His Asp Glu Gly Asp Pro Met Gln Thr Thr Asp Pro Asp Pro Thr         
            100                 105                 110                 

ctg acc ctg acc att gag aaa tgg aac tac cag acc cct agc att att      384
Leu Thr Leu Thr Ile Glu Lys Trp Asn Tyr Gln Thr Pro Ser Ile Ile         
        115                 120                 125                     

ttc cca gaa aac gac cag acc tac att gtg ctg agc gac cag cag cct      432
Phe Pro Glu Asn Asp Gln Thr Tyr Ile Val Leu Ser Asp Gln Gln Pro         
    130                 135                 140                         

ggt cag cca ctg gca ctg ttt aac aac act ggt aca tcc aac act ctg      480
Gly Gln Pro Leu Ala Leu Phe Asn Asn Thr Gly Thr Ser Asn Thr Leu         
145                 150                 155                 160         

cca gac ttc tca gct act gat ggt gag act aaa gac tat agc aaa tgg      528
Pro Asp Phe Ser Ala Thr Asp Gly Glu Thr Lys Asp Tyr Ser Lys Trp         
                165                 170                 175             

gat gta aag ttc agc tac acc cag acc aac tat gaa gat gac aag atc      576
Asp Val Lys Phe Ser Tyr Thr Gln Thr Asn Tyr Glu Asp Asp Lys Ile         
            180                 185                 190                 

ttt gtt att gat cac atc cag cca tgc gtt tcc cag ctt cag gtt agc      624
Phe Val Ile Asp His Ile Gln Pro Cys Val Ser Gln Leu Gln Val Ser         
        195                 200                 205                     

aaa cac ttc aac tct gac ctg gtt cgc tcc aag aaa tac aaa ctg act      672
Lys His Phe Asn Ser Asp Leu Val Arg Ser Lys Lys Tyr Lys Leu Thr         
    210                 215                 220                         

atc act gct tct gtg aag gat ggt gct gaa cag gaa ggt gag gct ggc      720
Ile Thr Ala Ser Val Lys Asp Gly Ala Glu Gln Glu Gly Glu Ala Gly         
225                 230                 235                 240         

tac tct act tct gca aac atc tct att gtt ttc ctg aac aac gat gct      768
Tyr Ser Thr Ser Ala Asn Ile Ser Ile Val Phe Leu Asn Asn Asp Ala         
                245                 250                 255             

cag cca atc ttt cag aac tct gac tgg tct gtt tcc ttt gtt gag ttc      816
Gln Pro Ile Phe Gln Asn Ser Asp Trp Ser Val Ser Phe Val Glu Phe         
            260                 265                 270                 

aac acc act cag cca gca aaa cca ctg gaa gaa cag gca gaa tat gaa      864
Asn Thr Thr Gln Pro Ala Lys Pro Leu Glu Glu Gln Ala Glu Tyr Glu         
        275                 280                 285                     

aac acc aag ggt ggc ctg ccg atc tat tac cat ttc tac tct gaa aac      912
Asn Thr Lys Gly Gly Leu Pro Ile Tyr Tyr His Phe Tyr Ser Glu Asn         
    290                 295                 300                         

cag acc ctg tcc aaa tac ttt gag gtt gat gag act tct ggt gac ctg      960
Gln Thr Leu Ser Lys Tyr Phe Glu Val Asp Glu Thr Ser Gly Asp Leu         
305                 310                 315                 320         

tct gtt att ggt aac ctg act tat gac tat gac cag gac atc agc ttt     1008
Ser Val Ile Gly Asn Leu Thr Tyr Asp Tyr Asp Gln Asp Ile Ser Phe         
                325                 330                 335             

cac att gtt gct tcc aac gat tct cag gta cgc atg ctg gac cca cgc     1056
His Ile Val Ala Ser Asn Asp Ser Gln Val Arg Met Leu Asp Pro Arg         
            340                 345                 350                 

tct tct ctg aac gta act gta aac ttc ctg cca cgt aac cgt cgt gcc     1104
Ser Ser Leu Asn Val Thr Val Asn Phe Leu Pro Arg Asn Arg Arg Ala         
        355                 360                 365                     

cca cag tgg cac cac cac cac cac cac taa                             1134
Pro Gln Trp His His His His His His                                     
    370                 375                                             


<210>  7
<211>  377
<212>  PRT
<213>  Diabrotica virgifera

<400>  7

Met Ala Val Leu Met Thr Lys Asp Leu Gln Cys Ser Glu Asn Leu Asn 
1               5                   10                  15      


Lys Asp Gly Glu Val Gly Glu Gly Ile Ile Gly Glu Asp Ile Asp Asp 
            20                  25                  30          


Gly Asp Asn Ala Lys Ile Asp Phe Ser Val Leu Ser Ile Val Asp Lys 
        35                  40                  45              


Glu Thr Lys Asn Asp Ile Gln Glu Ser Phe Asn Ile Ser Lys Ile Asp 
    50                  55                  60                  


Ser Asp Tyr Val Leu Asn Asp Thr Leu Lys Lys Val His Leu Ile Ala 
65                  70                  75                  80  


Phe Glu Asp Leu Lys Gly Lys Tyr Gly Thr Tyr Glu Val Thr Leu His 
                85                  90                  95      


Met His Asp Glu Gly Asp Pro Met Gln Thr Thr Asp Pro Asp Pro Thr 
            100                 105                 110         


Leu Thr Leu Thr Ile Glu Lys Trp Asn Tyr Gln Thr Pro Ser Ile Ile 
        115                 120                 125             


Phe Pro Glu Asn Asp Gln Thr Tyr Ile Val Leu Ser Asp Gln Gln Pro 
    130                 135                 140                 


Gly Gln Pro Leu Ala Leu Phe Asn Asn Thr Gly Thr Ser Asn Thr Leu 
145                 150                 155                 160 


Pro Asp Phe Ser Ala Thr Asp Gly Glu Thr Lys Asp Tyr Ser Lys Trp 
                165                 170                 175     


Asp Val Lys Phe Ser Tyr Thr Gln Thr Asn Tyr Glu Asp Asp Lys Ile 
            180                 185                 190         


Phe Val Ile Asp His Ile Gln Pro Cys Val Ser Gln Leu Gln Val Ser 
        195                 200                 205             


Lys His Phe Asn Ser Asp Leu Val Arg Ser Lys Lys Tyr Lys Leu Thr 
    210                 215                 220                 


Ile Thr Ala Ser Val Lys Asp Gly Ala Glu Gln Glu Gly Glu Ala Gly 
225                 230                 235                 240 


Tyr Ser Thr Ser Ala Asn Ile Ser Ile Val Phe Leu Asn Asn Asp Ala 
                245                 250                 255     


Gln Pro Ile Phe Gln Asn Ser Asp Trp Ser Val Ser Phe Val Glu Phe 
            260                 265                 270         


Asn Thr Thr Gln Pro Ala Lys Pro Leu Glu Glu Gln Ala Glu Tyr Glu 
        275                 280                 285             


Asn Thr Lys Gly Gly Leu Pro Ile Tyr Tyr His Phe Tyr Ser Glu Asn 
    290                 295                 300                 


Gln Thr Leu Ser Lys Tyr Phe Glu Val Asp Glu Thr Ser Gly Asp Leu 
305                 310                 315                 320 


Ser Val Ile Gly Asn Leu Thr Tyr Asp Tyr Asp Gln Asp Ile Ser Phe 
                325                 330                 335     


His Ile Val Ala Ser Asn Asp Ser Gln Val Arg Met Leu Asp Pro Arg 
            340                 345                 350         


Ser Ser Leu Asn Val Thr Val Asn Phe Leu Pro Arg Asn Arg Arg Ala 
        355                 360                 365             


Pro Gln Trp His His His His His His 
    370                 375         


<210>  8
<211>  639
<212>  DNA
<213>  Artificial sequence

<220>
<223>  version of SEQ ID NO: 9 with added cloning sites

<400>  8
gaattcatga aacagtggta tcagcgtcgt gttgccatca ccgcaggtaa agacaccacc     60

tctaaagaca aactgcagta caacatcgac aacatcaccc cgtccaacct ggacctggac    120

atcaaatccg cgttcactat gaacactcag agcggtgata tcaccatcaa ctttgaggtt    180

aaagactcta tggaaggcta cttcaccctg gacctgtccg tacaggatga agaaccggaa    240

aaccacaaag ctgacgcaac cctgaaaatc tacattgtaa cctctaagaa cactgtagtt    300

ttccgcttcg aaaacgacca ggaaactgtt agcgacaaag cgggggacat caaatctgta    360

ctcgacgaag aattccagta cgaaaccaaa gttgaagccc cgacaggtaa caccactgac    420

ggtacgccgc tgacccgtag cccggttttc ttcctgaacc tcaacaccaa tgaaccggtt    480

gacgctaccg agatcctgaa gaaagttact aacgtagatg ttttccagcg tctgaagaac    540

aacttctcta aagttggcct ggttctgctg tccttcgact ctagctctga gactaacgaa    600

aacctggagg cacatcatca tcatcatcat taaaagctt                           639


<210>  9
<211>  627
<212>  DNA
<213>  Tenebrio molitor


<220>
<221>  CDS
<222>  (1)..(624)

<400>  9
atg aaa cag tgg tat cag cgt cgt gtt gcc atc acc gca ggt aaa gac       48
Met Lys Gln Trp Tyr Gln Arg Arg Val Ala Ile Thr Ala Gly Lys Asp         
1               5                   10                  15              

acc acc tct aaa gac aaa ctg cag tac aac atc gac aac atc acc ccg       96
Thr Thr Ser Lys Asp Lys Leu Gln Tyr Asn Ile Asp Asn Ile Thr Pro         
            20                  25                  30                  

tcc aac ctg gac ctg gac atc aaa tcc gcg ttc act atg aac act cag      144
Ser Asn Leu Asp Leu Asp Ile Lys Ser Ala Phe Thr Met Asn Thr Gln         
        35                  40                  45                      

agc ggt gat atc acc atc aac ttt gag gtt aaa gac tct atg gaa ggc      192
Ser Gly Asp Ile Thr Ile Asn Phe Glu Val Lys Asp Ser Met Glu Gly         
    50                  55                  60                          

tac ttc acc ctg gac ctg tcc gta cag gat gaa gaa ccg gaa aac cac      240
Tyr Phe Thr Leu Asp Leu Ser Val Gln Asp Glu Glu Pro Glu Asn His         
65                  70                  75                  80          

aaa gct gac gca acc ctg aaa atc tac att gta acc tct aag aac act      288
Lys Ala Asp Ala Thr Leu Lys Ile Tyr Ile Val Thr Ser Lys Asn Thr         
                85                  90                  95              

gta gtt ttc cgc ttc gaa aac gac cag gaa act gtt agc gac aaa gcg      336
Val Val Phe Arg Phe Glu Asn Asp Gln Glu Thr Val Ser Asp Lys Ala         
            100                 105                 110                 

ggg gac atc aaa tct gta ctc gac gaa gaa ttc cag tac gaa acc aaa      384
Gly Asp Ile Lys Ser Val Leu Asp Glu Glu Phe Gln Tyr Glu Thr Lys         
        115                 120                 125                     

gtt gaa gcc ccg aca ggt aac acc act gac ggt acg ccg ctg acc cgt      432
Val Glu Ala Pro Thr Gly Asn Thr Thr Asp Gly Thr Pro Leu Thr Arg         
    130                 135                 140                         

agc ccg gtt ttc ttc ctg aac ctc aac acc aat gaa ccg gtt gac gct      480
Ser Pro Val Phe Phe Leu Asn Leu Asn Thr Asn Glu Pro Val Asp Ala         
145                 150                 155                 160         

acc gag atc ctg aag aaa gtt act aac gta gat gtt ttc cag cgt ctg      528
Thr Glu Ile Leu Lys Lys Val Thr Asn Val Asp Val Phe Gln Arg Leu         
                165                 170                 175             

aag aac aac ttc tct aaa gtt ggc ctg gtt ctg ctg tcc ttc gac tct      576
Lys Asn Asn Phe Ser Lys Val Gly Leu Val Leu Leu Ser Phe Asp Ser         
            180                 185                 190                 

agc tct gag act aac gaa aac ctg gag gca cat cat cat cat cat cat      624
Ser Ser Glu Thr Asn Glu Asn Leu Glu Ala His His His His His His         
        195                 200                 205                     

taa                                                                  627


<210>  10
<211>  208
<212>  PRT
<213>  Tenebrio molitor

<400>  10

Met Lys Gln Trp Tyr Gln Arg Arg Val Ala Ile Thr Ala Gly Lys Asp 
1               5                   10                  15      


Thr Thr Ser Lys Asp Lys Leu Gln Tyr Asn Ile Asp Asn Ile Thr Pro 
            20                  25                  30          


Ser Asn Leu Asp Leu Asp Ile Lys Ser Ala Phe Thr Met Asn Thr Gln 
        35                  40                  45              


Ser Gly Asp Ile Thr Ile Asn Phe Glu Val Lys Asp Ser Met Glu Gly 
    50                  55                  60                  


Tyr Phe Thr Leu Asp Leu Ser Val Gln Asp Glu Glu Pro Glu Asn His 
65                  70                  75                  80  


Lys Ala Asp Ala Thr Leu Lys Ile Tyr Ile Val Thr Ser Lys Asn Thr 
                85                  90                  95      


Val Val Phe Arg Phe Glu Asn Asp Gln Glu Thr Val Ser Asp Lys Ala 
            100                 105                 110         


Gly Asp Ile Lys Ser Val Leu Asp Glu Glu Phe Gln Tyr Glu Thr Lys 
        115                 120                 125             


Val Glu Ala Pro Thr Gly Asn Thr Thr Asp Gly Thr Pro Leu Thr Arg 
    130                 135                 140                 


Ser Pro Val Phe Phe Leu Asn Leu Asn Thr Asn Glu Pro Val Asp Ala 
145                 150                 155                 160 


Thr Glu Ile Leu Lys Lys Val Thr Asn Val Asp Val Phe Gln Arg Leu 
                165                 170                 175     


Lys Asn Asn Phe Ser Lys Val Gly Leu Val Leu Leu Ser Phe Asp Ser 
            180                 185                 190         


Ser Ser Glu Thr Asn Glu Asn Leu Glu Ala His His His His His His 
        195                 200                 205             


<210>  11
<211>  1134
<212>  DNA
<213>  Artificial sequence

<220>
<223>  protease-stabilized version of SEQ ID NOs: 6 and 7


<220>
<221>  CDS
<222>  (1)..(1131)

<400>  11
atg gct gta ctg atg acc tgt gac ctg cag tgt tct gaa aac ctg aac       48
Met Ala Val Leu Met Thr Cys Asp Leu Gln Cys Ser Glu Asn Leu Asn         
1               5                   10                  15              

tct gat ggt gaa gtt ggt gag ggt atc att ggt gaa gac att gat gat       96
Ser Asp Gly Glu Val Gly Glu Gly Ile Ile Gly Glu Asp Ile Asp Asp         
            20                  25                  30                  

ggt gac aat gct tgc att gac ttc tct gtt ctg tct att gtt gac agt      144
Gly Asp Asn Ala Cys Ile Asp Phe Ser Val Leu Ser Ile Val Asp Ser         
        35                  40                  45                      

gaa act tct aat gac atc cag gaa tcc ttc aac atc tct tgc att gac      192
Glu Thr Ser Asn Asp Ile Gln Glu Ser Phe Asn Ile Ser Cys Ile Asp         
    50                  55                  60                          

tct gac tat gtc ctg aat gat acc ctg tct tct gtt cac ctc att gct      240
Ser Asp Tyr Val Leu Asn Asp Thr Leu Ser Ser Val His Leu Ile Ala         
65                  70                  75                  80          

ttt gag gat ctg tct ggt tcc tat ggc acc tat gaa gta acc ctg cac      288
Phe Glu Asp Leu Ser Gly Ser Tyr Gly Thr Tyr Glu Val Thr Leu His         
                85                  90                  95              

atg cat gat gaa ggt gac cca atg cag act act gac cca gac cct acc      336
Met His Asp Glu Gly Asp Pro Met Gln Thr Thr Asp Pro Asp Pro Thr         
            100                 105                 110                 

ctg acc ctg act att gaa tct tgg aat tac cag act cca tct atc atc      384
Leu Thr Leu Thr Ile Glu Ser Trp Asn Tyr Gln Thr Pro Ser Ile Ile         
        115                 120                 125                     

ttc cca gag aat gac cag acc tac att gta ctg tct gac cag caa cca      432
Phe Pro Glu Asn Asp Gln Thr Tyr Ile Val Leu Ser Asp Gln Gln Pro         
    130                 135                 140                         

ggc cag cct ctg gct ctg ttt aac aac act ggc acc tct aac act ctc      480
Gly Gln Pro Leu Ala Leu Phe Asn Asn Thr Gly Thr Ser Asn Thr Leu         
145                 150                 155                 160         

cca gac ttc agt gct act gat ggt gaa act tct gac tat tcc tgc tgg      528
Pro Asp Phe Ser Ala Thr Asp Gly Glu Thr Ser Asp Tyr Ser Cys Trp         
                165                 170                 175             

gat gtt tct ttc agc tac acc cag act aac tat gaa gat gac tca atc      576
Asp Val Ser Phe Ser Tyr Thr Gln Thr Asn Tyr Glu Asp Asp Ser Ile         
            180                 185                 190                 

ttt gtt att gac cac atc cag cca tgt gtg tcc cag ctg cag gtt tct      624
Phe Val Ile Asp His Ile Gln Pro Cys Val Ser Gln Leu Gln Val Ser         
        195                 200                 205                     

gct cac ttc aac tct gat ctg gta gct tct tgc tct tat gct ctg acc      672
Ala His Phe Asn Ser Asp Leu Val Ala Ser Cys Ser Tyr Ala Leu Thr         
    210                 215                 220                         

atc act gct tct gtt gct gat ggt gca gaa caa gaa ggt gaa gct ggt      720
Ile Thr Ala Ser Val Ala Asp Gly Ala Glu Gln Glu Gly Glu Ala Gly         
225                 230                 235                 240         

tat tcc act tct gct aac att tct att gta ttc ctg aac aac gat gca      768
Tyr Ser Thr Ser Ala Asn Ile Ser Ile Val Phe Leu Asn Asn Asp Ala         
                245                 250                 255             

cag cca atc ttc cag aac tct gac tgg agt gtt agc ttt gtt gaa ttt      816
Gln Pro Ile Phe Gln Asn Ser Asp Trp Ser Val Ser Phe Val Glu Phe         
            260                 265                 270                 

aac acc act cag cca gca tct cct ctt gaa gaa cag gct gaa tat gag      864
Asn Thr Thr Gln Pro Ala Ser Pro Leu Glu Glu Gln Ala Glu Tyr Glu         
        275                 280                 285                     

aac acc tct ggt ggt ctg cca atc tac tac cac ttc tat tct gaa aac      912
Asn Thr Ser Gly Gly Leu Pro Ile Tyr Tyr His Phe Tyr Ser Glu Asn         
    290                 295                 300                         

cag acc ctg tcc tgt tac ttt gaa gta gat gag acc agt ggt gat ctg      960
Gln Thr Leu Ser Cys Tyr Phe Glu Val Asp Glu Thr Ser Gly Asp Leu         
305                 310                 315                 320         

agt gtg att ggt aac ctg act tat gac tat gat caa gac atc tct ttc     1008
Ser Val Ile Gly Asn Leu Thr Tyr Asp Tyr Asp Gln Asp Ile Ser Phe         
                325                 330                 335             

cac att gtt gca tct aat gac tcc cag gta tcc atg ctg gac cca tgc     1056
His Ile Val Ala Ser Asn Asp Ser Gln Val Ser Met Leu Asp Pro Cys         
            340                 345                 350                 

tct tct ctg aac gtt act gtt aac ttc ctg cct gct aac aga cgt gct     1104
Ser Ser Leu Asn Val Thr Val Asn Phe Leu Pro Ala Asn Arg Arg Ala         
        355                 360                 365                     

cca cag tgg cac cac cac cac cat cac taa                             1134
Pro Gln Trp His His His His His His                                     
    370                 375                                             


<210>  12
<211>  377
<212>  PRT
<213>  Artificial sequence

<220>
<223>  protease-stabilized version of SEQ ID NOs: 6 and 7

<400>  12

Met Ala Val Leu Met Thr Cys Asp Leu Gln Cys Ser Glu Asn Leu Asn 
1               5                   10                  15      


Ser Asp Gly Glu Val Gly Glu Gly Ile Ile Gly Glu Asp Ile Asp Asp 
            20                  25                  30          


Gly Asp Asn Ala Cys Ile Asp Phe Ser Val Leu Ser Ile Val Asp Ser 
        35                  40                  45              


Glu Thr Ser Asn Asp Ile Gln Glu Ser Phe Asn Ile Ser Cys Ile Asp 
    50                  55                  60                  


Ser Asp Tyr Val Leu Asn Asp Thr Leu Ser Ser Val His Leu Ile Ala 
65                  70                  75                  80  


Phe Glu Asp Leu Ser Gly Ser Tyr Gly Thr Tyr Glu Val Thr Leu His 
                85                  90                  95      


Met His Asp Glu Gly Asp Pro Met Gln Thr Thr Asp Pro Asp Pro Thr 
            100                 105                 110         


Leu Thr Leu Thr Ile Glu Ser Trp Asn Tyr Gln Thr Pro Ser Ile Ile 
        115                 120                 125             


Phe Pro Glu Asn Asp Gln Thr Tyr Ile Val Leu Ser Asp Gln Gln Pro 
    130                 135                 140                 


Gly Gln Pro Leu Ala Leu Phe Asn Asn Thr Gly Thr Ser Asn Thr Leu 
145                 150                 155                 160 


Pro Asp Phe Ser Ala Thr Asp Gly Glu Thr Ser Asp Tyr Ser Cys Trp 
                165                 170                 175     


Asp Val Ser Phe Ser Tyr Thr Gln Thr Asn Tyr Glu Asp Asp Ser Ile 
            180                 185                 190         


Phe Val Ile Asp His Ile Gln Pro Cys Val Ser Gln Leu Gln Val Ser 
        195                 200                 205             


Ala His Phe Asn Ser Asp Leu Val Ala Ser Cys Ser Tyr Ala Leu Thr 
    210                 215                 220                 


Ile Thr Ala Ser Val Ala Asp Gly Ala Glu Gln Glu Gly Glu Ala Gly 
225                 230                 235                 240 


Tyr Ser Thr Ser Ala Asn Ile Ser Ile Val Phe Leu Asn Asn Asp Ala 
                245                 250                 255     


Gln Pro Ile Phe Gln Asn Ser Asp Trp Ser Val Ser Phe Val Glu Phe 
            260                 265                 270         


Asn Thr Thr Gln Pro Ala Ser Pro Leu Glu Glu Gln Ala Glu Tyr Glu 
        275                 280                 285             


Asn Thr Ser Gly Gly Leu Pro Ile Tyr Tyr His Phe Tyr Ser Glu Asn 
    290                 295                 300                 


Gln Thr Leu Ser Cys Tyr Phe Glu Val Asp Glu Thr Ser Gly Asp Leu 
305                 310                 315                 320 


Ser Val Ile Gly Asn Leu Thr Tyr Asp Tyr Asp Gln Asp Ile Ser Phe 
                325                 330                 335     


His Ile Val Ala Ser Asn Asp Ser Gln Val Ser Met Leu Asp Pro Cys 
            340                 345                 350         


Ser Ser Leu Asn Val Thr Val Asn Phe Leu Pro Ala Asn Arg Arg Ala 
        355                 360                 365             


Pro Gln Trp His His His His His His 
    370                 375         


