                         SEQUENCE LISTING

<110>  University of Kentucky Research Foundation
       Daunert, Sylvia
       Turner, Kendrick
       Joel, Smita
       Rowe, Laura
 
<120>  POLYPEPTIDES, SYSTEMS, AND METHODS USEFUL FOR DETECTING GLUCOSE

<130>  13177N/1499PCT

<150>  60/954,269
<151>  2007-08-06

<150>  60/954,348
<151>  2007-08-07

<160>  7     

<170>  PatentIn version 3.5

<210>  1
<211>  309
<212>  PRT
<213>  Escherichia coli

<400>  1

Ala Asp Thr Arg Ile Gly Val Thr Ile Tyr Lys Tyr Asp Asp Asn Phe 
1               5                   10                  15      


Met Ser Val Val Arg Lys Ala Ile Glu Gln Asp Ala Lys Ala Ala Pro 
            20                  25                  30          


Asp Val Gln Leu Leu Met Asn Asp Ser Gln Asn Asp Gln Ser Lys Gln 
        35                  40                  45              


Asn Asp Gln Ile Asp Val Leu Leu Ala Lys Gly Val Lys Ala Leu Ala 
    50                  55                  60                  


Ile Asn Leu Val Asp Pro Ala Ala Ala Gly Thr Val Ile Glu Lys Ala 
65                  70                  75                  80  


Arg Gly Gln Asn Val Pro Val Val Phe Phe Asn Lys Glu Pro Ser Arg 
                85                  90                  95      


Lys Ala Leu Asp Ser Tyr Asp Lys Ala Tyr Tyr Val Gly Thr Asp Ser 
            100                 105                 110         


Lys Glu Ser Gly Ile Ile Gln Gly Asp Leu Ile Ala Lys His Trp Ala 
        115                 120                 125             


Ala Asn Gln Gly Trp Asp Leu Asn Lys Asp Gly Gln Ile Gln Phe Val 
    130                 135                 140                 


Leu Leu Lys Gly Glu Pro Gly His Pro Asp Ala Glu Ala Arg Thr Thr 
145                 150                 155                 160 


Tyr Val Ile Lys Glu Leu Asn Asp Lys Gly Ile Lys Thr Glu Gln Leu 
                165                 170                 175     


Gln Leu Asp Thr Ala Met Trp Asp Thr Ala Gln Ala Lys Asp Lys Met 
            180                 185                 190         


Asp Ala Trp Leu Ser Gly Pro Asn Ala Asn Lys Ile Glu Val Val Ile 
        195                 200                 205             


Ala Asn Asn Asp Ala Met Ala Met Gly Ala Val Glu Ala Leu Lys Ala 
    210                 215                 220                 


His Asn Lys Ser Ser Ile Pro Val Phe Gly Val Asp Ala Leu Pro Glu 
225                 230                 235                 240 


Ala Leu Ala Leu Val Lys Ser Gly Ala Leu Ala Gly Thr Val Leu Asn 
                245                 250                 255     


Asp Ala Asn Asn Gln Ala Lys Ala Thr Phe Asp Leu Ala Lys Asn Leu 
            260                 265                 270         


Ala Asp Gly Lys Gly Ala Ala Asp Gly Thr Asn Trp Lys Ile Asp Asn 
        275                 280                 285             


Lys Val Val Arg Val Pro Tyr Val Gly Val Asp Lys Asp Asn Leu Ala 
    290                 295                 300                 


Glu Phe Ser Lys Lys 
305                 


<210>  2
<211>  283
<212>  PRT
<213>  Escherichia coli

<400>  2

Asp Asn Phe Met Ser Val Val Arg Lys Ala Ile Glu Gln Asp Ala Lys 
1               5                   10                  15      


Ala Ala Pro Asp Val Gln Leu Leu Met Asn Asp Ser Gln Asn Asp Gln 
            20                  25                  30          


Ser Lys Gln Asn Asp Gln Ile Asp Val Leu Leu Ala Lys Gly Val Lys 
        35                  40                  45              


Ala Leu Ala Ile Asn Leu Val Asp Pro Ala Ala Ala Gly Thr Val Ile 
    50                  55                  60                  


Glu Lys Ala Arg Gly Gln Asn Val Pro Val Val Phe Phe Asn Lys Glu 
65                  70                  75                  80  


Pro Ser Arg Lys Ala Leu Asp Ser Tyr Asp Lys Ala Tyr Tyr Val Gly 
                85                  90                  95      


Thr Asp Ser Lys Glu Ser Gly Ile Ile Gln Gly Asp Leu Ile Ala Lys 
            100                 105                 110         


His Trp Ala Ala Asn Gln Gly Trp Asp Leu Asn Lys Asp Gly Gln Ile 
        115                 120                 125             


Gln Phe Val Leu Leu Lys Gly Glu Pro Gly His Pro Asp Ala Glu Ala 
    130                 135                 140                 


Arg Thr Thr Tyr Val Ile Lys Glu Leu Asn Asp Lys Gly Ile Lys Thr 
145                 150                 155                 160 


Glu Gln Leu Gln Leu Asp Thr Ala Met Trp Asp Thr Ala Gln Ala Lys 
                165                 170                 175     


Asp Lys Met Asp Ala Trp Leu Ser Gly Pro Asn Ala Asn Lys Ile Glu 
            180                 185                 190         


Val Val Ile Ala Asn Asn Asp Ala Met Ala Met Gly Ala Val Glu Ala 
        195                 200                 205             


Leu Lys Ala His Asn Lys Ser Ser Ile Pro Val Phe Gly Val Asp Ala 
    210                 215                 220                 


Leu Pro Glu Ala Leu Ala Leu Val Lys Ser Gly Ala Leu Ala Gly Thr 
225                 230                 235                 240 


Val Leu Asn Asp Ala Asn Asn Gln Ala Lys Ala Thr Phe Asp Leu Ala 
                245                 250                 255     


Lys Asn Leu Ala Asp Gly Lys Gly Ala Ala Asp Gly Thr Asn Trp Lys 
            260                 265                 270         


Ile Asp Asn Lys Val Val Arg Val Pro Tyr Val 
        275                 280             


<210>  3
<211>  243
<212>  PRT
<213>  Escherichia coli

<400>  3

Asp Asn Phe Met Ser Val Val Arg Lys Ala Ile Glu Gln Asp Ala Lys 
1               5                   10                  15      


Ala Ala Pro Asp Val Gln Leu Leu Met Asn Asp Ser Gln Asn Asp Gln 
            20                  25                  30          


Ser Lys Gln Asn Asp Gln Ile Asp Val Leu Leu Ala Lys Gly Val Lys 
        35                  40                  45              


Ala Leu Ala Ile Asn Leu Val Asp Pro Ala Ala Ala Gly Thr Val Ile 
    50                  55                  60                  


Glu Lys Ala Arg Gly Gln Asn Val Pro Val Val Phe Phe Asn Lys Glu 
65                  70                  75                  80  


Pro Ser Arg Lys Ala Leu Asp Ser Tyr Asp Lys Ala Tyr Tyr Val Gly 
                85                  90                  95      


Thr Asp Ser Lys Glu Ser Gly Ile Ile Gln Gly Asp Leu Ile Ala Lys 
            100                 105                 110         


His Trp Ala Ala Asn Gln Gly Trp Asp Leu Asn Lys Asp Gly Gln Ile 
        115                 120                 125             


Gln Phe Val Leu Leu Lys Gly Glu Pro Gly His Pro Asp Ala Glu Ala 
    130                 135                 140                 


Arg Thr Thr Tyr Val Ile Lys Glu Leu Asn Asp Lys Gly Ile Lys Thr 
145                 150                 155                 160 


Glu Gln Leu Gln Leu Asp Thr Ala Met Trp Asp Thr Ala Gln Ala Lys 
                165                 170                 175     


Asp Lys Met Asp Ala Trp Leu Ser Gly Pro Asn Ala Asn Lys Ile Glu 
            180                 185                 190         


Val Val Ile Ala Asn Asn Asp Ala Met Ala Met Gly Ala Val Glu Ala 
        195                 200                 205             


Leu Lys Ala His Asn Lys Ser Ser Ile Pro Val Phe Gly Val Asp Ala 
    210                 215                 220                 


Leu Pro Glu Ala Leu Ala Leu Val Lys Ser Gly Ala Leu Ala Gly Thr 
225                 230                 235                 240 


Val Leu Asn 
            


<210>  4
<211>  213
<212>  PRT
<213>  Escherichia coli

<400>  4

Asp Asn Phe Met Ser Val Val Arg Lys Ala Ile Glu Gln Asp Ala Lys 
1               5                   10                  15      


Ala Ala Pro Asp Val Gln Leu Leu Met Asn Asp Ser Gln Asn Asp Gln 
            20                  25                  30          


Ser Lys Gln Asn Asp Gln Ile Asp Val Leu Leu Ala Lys Gly Val Lys 
        35                  40                  45              


Ala Leu Ala Ile Asn Leu Val Asp Pro Ala Ala Ala Gly Thr Val Ile 
    50                  55                  60                  


Glu Lys Ala Arg Gly Gln Asn Val Pro Val Val Phe Phe Asn Lys Glu 
65                  70                  75                  80  


Pro Ser Arg Lys Ala Leu Asp Ser Tyr Asp Lys Ala Tyr Tyr Val Gly 
                85                  90                  95      


Thr Asp Ser Lys Glu Ser Gly Ile Ile Gln Gly Asp Leu Ile Ala Lys 
            100                 105                 110         


His Trp Ala Ala Asn Gln Gly Trp Asp Leu Asn Lys Asp Gly Gln Ile 
        115                 120                 125             


Gln Phe Val Leu Leu Lys Gly Glu Pro Gly His Pro Asp Ala Glu Ala 
    130                 135                 140                 


Arg Thr Thr Tyr Val Ile Lys Glu Leu Asn Asp Lys Gly Ile Lys Thr 
145                 150                 155                 160 


Glu Gln Leu Gln Leu Asp Thr Ala Met Trp Asp Thr Ala Gln Ala Lys 
                165                 170                 175     


Asp Lys Met Asp Ala Trp Leu Ser Gly Pro Asn Ala Asn Lys Ile Glu 
            180                 185                 190         


Val Val Ile Ala Asn Asn Asp Ala Met Ala Met Gly Ala Val Glu Ala 
        195                 200                 205             


Leu Lys Ala His Asn 
    210             


<210>  5
<211>  304
<212>  PRT
<213>  Thermotoga maritima

<400>  5

Leu Thr Ile Gly Val Ile Gly Lys Ser Val His Pro Tyr Trp Ser Gln 
1               5                   10                  15      


Val Glu Gln Gly Val Lys Ala Ala Gly Lys Ala Leu Gly Val Asp Thr 
            20                  25                  30          


Lys Phe Phe Val Pro Gln Lys Glu Asp Ile Asn Ala Gln Leu Gln Met 
        35                  40                  45              


Leu Glu Ser Phe Ile Ala Glu Gly Val Asn Gly Ile Ala Ile Ala Pro 
    50                  55                  60                  


Ser Asp Pro Thr Ala Val Ile Pro Thr Ile Lys Lys Ala Leu Glu Met 
65                  70                  75                  80  


Gly Ile Pro Val Val Thr Leu Asp Thr Asp Ser Pro Asp Ser Gly Arg 
                85                  90                  95      


Tyr Val Tyr Ile Gly Thr Asp Asn Tyr Gln Ala Gly Tyr Thr Ala Gly 
            100                 105                 110         


Leu Ile Met Lys Glu Leu Leu Gly Gly Lys Gly Lys Val Val Ile Gly 
        115                 120                 125             


Thr Gly Ser Leu Thr Ala Met Asn Ser Leu Gln Arg Ile Gln Gly Phe 
    130                 135                 140                 


Lys Asp Ala Ile Lys Asp Ser Glu Ile Glu Ile Val Asp Ile Leu Asn 
145                 150                 155                 160 


Asp Glu Glu Asp Gly Ala Arg Ala Val Ser Leu Ala Glu Ala Ala Leu 
                165                 170                 175     


Asn Ala His Pro Asp Leu Asp Ala Phe Phe Gly Val Tyr Ala Tyr Asn 
            180                 185                 190         


Gly Pro Ala Gln Ala Leu Val Val Lys Asn Ala Gly Lys Val Gly Lys 
        195                 200                 205             


Val Lys Ile Val Cys Phe Asp Thr Thr Pro Asp Ile Leu Gln Tyr Val 
    210                 215                 220                 


Lys Glu Gly Val Ile Gln Ala Thr Met Gly Gln Arg Pro Tyr Met Met 
225                 230                 235                 240 


Gly Tyr Leu Ser Val Thr Val Leu Tyr Leu Met Asn Lys Ile Gly Val 
                245                 250                 255     


Gln Asn Thr Leu Met Met Leu Pro Lys Val Lys Val Asp Gly Lys Val 
            260                 265                 270         


Asp Tyr Val Ile Asp Thr Gly Val Asp Val Val Thr Pro Glu Asn Leu 
        275                 280                 285             


Asp Glu Tyr Leu Lys Lys Met Glu Glu Leu Gly Ile Pro Ile Lys Phe 
    290                 295                 300                 


<210>  6
<211>  241
<212>  PRT
<213>  Thermotoga maritima

<400>  6

Tyr Trp Ser Gln Val Glu Gln Gly Val Lys Ala Ala Gly Lys Ala Leu 
1               5                   10                  15      


Gly Val Asp Thr Lys Phe Phe Val Pro Gln Lys Glu Asp Ile Asn Ala 
            20                  25                  30          


Gln Leu Gln Met Leu Glu Ser Phe Ile Ala Glu Gly Val Asn Gly Ile 
        35                  40                  45              


Ala Ile Ala Pro Ser Asp Pro Thr Ala Val Ile Pro Thr Ile Lys Lys 
    50                  55                  60                  


Ala Leu Glu Met Gly Ile Pro Val Val Thr Leu Asp Thr Asp Ser Pro 
65                  70                  75                  80  


Asp Ser Gly Arg Tyr Val Tyr Ile Gly Thr Asp Asn Tyr Gln Ala Gly 
                85                  90                  95      


Tyr Thr Ala Gly Leu Ile Met Lys Glu Leu Leu Gly Gly Lys Gly Lys 
            100                 105                 110         


Val Val Ile Gly Thr Gly Ser Leu Thr Ala Met Asn Ser Leu Gln Arg 
        115                 120                 125             


Ile Gln Gly Phe Lys Asp Ala Ile Lys Asp Ser Glu Ile Glu Ile Val 
    130                 135                 140                 


Asp Ile Leu Asn Asp Glu Glu Asp Gly Ala Arg Ala Val Ser Leu Ala 
145                 150                 155                 160 


Glu Ala Ala Leu Asn Ala His Pro Asp Leu Asp Ala Phe Phe Gly Val 
                165                 170                 175     


Tyr Ala Tyr Asn Gly Pro Ala Gln Ala Leu Val Val Lys Asn Ala Gly 
            180                 185                 190         


Lys Val Gly Lys Val Lys Ile Val Cys Phe Asp Thr Thr Pro Asp Ile 
        195                 200                 205             


Leu Gln Tyr Val Lys Glu Gly Val Ile Gln Ala Thr Met Gly Gln Arg 
    210                 215                 220                 


Pro Tyr Met Met Gly Tyr Leu Ser Val Thr Val Leu Tyr Leu Met Asn 
225                 230                 235                 240 


Lys 
    


<210>  7
<211>  193
<212>  PRT
<213>  Thermotoga maritima

<400>  7

Tyr Trp Ser Gln Val Glu Gln Gly Val Lys Ala Ala Gly Lys Ala Leu 
1               5                   10                  15      


Gly Val Asp Thr Lys Phe Phe Val Pro Gln Lys Glu Asp Ile Asn Ala 
            20                  25                  30          


Gln Leu Gln Met Leu Glu Ser Phe Ile Ala Glu Gly Val Asn Gly Ile 
        35                  40                  45              


Ala Ile Ala Pro Ser Asp Pro Thr Ala Val Ile Pro Thr Ile Lys Lys 
    50                  55                  60                  


Ala Leu Glu Met Gly Ile Pro Val Val Thr Leu Asp Thr Asp Ser Pro 
65                  70                  75                  80  


Asp Ser Gly Arg Tyr Val Tyr Ile Gly Thr Asp Asn Tyr Gln Ala Gly 
                85                  90                  95      


Tyr Thr Ala Gly Leu Ile Met Lys Glu Leu Leu Gly Gly Lys Gly Lys 
            100                 105                 110         


Val Val Ile Gly Thr Gly Ser Leu Thr Ala Met Asn Ser Leu Gln Arg 
        115                 120                 125             


Ile Gln Gly Phe Lys Asp Ala Ile Lys Asp Ser Glu Ile Glu Ile Val 
    130                 135                 140                 


Asp Ile Leu Asn Asp Glu Glu Asp Gly Ala Arg Ala Val Ser Leu Ala 
145                 150                 155                 160 


Glu Ala Ala Leu Asn Ala His Pro Asp Leu Asp Ala Phe Phe Gly Val 
                165                 170                 175     


Tyr Ala Tyr Asn Gly Pro Ala Gln Ala Leu Val Val Lys Asn Ala Gly 
            180                 185                 190         


Lys 
    


