                         SEQUENCE LISTING

<110>  The Government of the United States of America as 
       Represented by the Secretary of Health and Human Services
       Caughey, Byron W
       Atarashi, Ryuichiro
       Moore, Roger A.
       Priola, Suzette
 
<120>  DETECTION OF INFECTIOUS PRION PROTEIN BY SEEDED CONVERSION OF 
       RECOMBINANT PRION PROTEIN

<130>  4239-77856-03

<150>  US 60/961,364
<151>  2007-07-20

<150>  US 61/021,865
<151>  2008-01-17

<160>  11    

<170>  PatentIn version 3.5

<210>  1
<211>  209
<212>  PRT
<213>  Mesocricetus auratus

<400>  1

Lys Lys Arg Pro Lys Pro Gly Gly Trp Asn Thr Gly Gly Ser Arg Tyr 
1               5                   10                  15      


Pro Gly Gln Gly Ser Pro Gly Gly Asn Arg Tyr Pro Pro Gln Gly Gly 
            20                  25                  30          


Gly Thr Trp Gly Gln Pro His Gly Gly Gly Trp Gly Gln Pro His Gly 
        35                  40                  45              


Gly Gly Trp Gly Gln Pro His Gly Gly Gly Trp Gly Gln Pro His Gly 
    50                  55                  60                  


Gly Gly Trp Gly Gln Gly Gly Gly Thr His Asn Gln Trp Asn Lys Pro 
65                  70                  75                  80  


Asn Lys Pro Lys Thr Ser Met Lys His Met Ala Gly Ala Ala Ala Ala 
                85                  90                  95      


Gly Ala Val Val Gly Gly Leu Gly Gly Tyr Met Leu Gly Ser Ala Met 
            100                 105                 110         


Ser Arg Pro Met Leu His Phe Gly Asn Asp Trp Glu Asp Arg Tyr Tyr 
        115                 120                 125             


Arg Glu Asn Met Asn Arg Tyr Pro Asn Gln Val Tyr Tyr Arg Pro Val 
    130                 135                 140                 


Asp Gln Tyr Asn Asn Gln Asn Asn Phe Val His Asp Cys Val Asn Ile 
145                 150                 155                 160 


Thr Ile Lys Gln His Thr Val Thr Thr Thr Thr Lys Gly Glu Asn Phe 
                165                 170                 175     


Thr Glu Thr Asp Val Lys Met Met Glu Arg Val Val Glu Gln Met Cys 
            180                 185                 190         


Val Thr Gln Tyr Gln Lys Glu Ser Gln Ala Tyr Tyr Asp Gly Arg Arg 
        195                 200                 205             


Ser 
    


<210>  2
<211>  208
<212>  PRT
<213>  Mus musculus

<400>  2

Lys Lys Arg Pro Lys Pro Gly Gly Trp Asn Thr Gly Gly Ser Arg Tyr 
1               5                   10                  15      


Pro Gly Gln Gly Ser Pro Gly Gly Asn Arg Tyr Pro Pro Gln Gly Gly 
            20                  25                  30          


Thr Trp Gly Gln Pro His Gly Gly Gly Trp Gly Gln Pro His Gly Gly 
        35                  40                  45              


Ser Trp Gly Gln Pro His Gly Gly Ser Trp Gly Gln Pro His Gly Gly 
    50                  55                  60                  


Gly Trp Gly Gln Gly Gly Gly Thr His Asn Gln Trp Asn Lys Pro Ser 
65                  70                  75                  80  


Lys Pro Lys Thr Asn Leu Lys His Val Ala Gly Ala Ala Ala Ala Gly 
                85                  90                  95      


Ala Val Val Gly Gly Leu Gly Gly Tyr Met Leu Gly Ser Ala Met Ser 
            100                 105                 110         


Arg Pro Met Ile His Phe Gly Asn Asp Trp Glu Asp Arg Tyr Tyr Arg 
        115                 120                 125             


Glu Asn Met Tyr Arg Tyr Pro Asn Gln Val Tyr Tyr Arg Pro Val Asp 
    130                 135                 140                 


Gln Tyr Ser Asn Gln Asn Asn Phe Val His Asp Cys Val Asn Ile Thr 
145                 150                 155                 160 


Ile Lys Gln His Thr Val Thr Thr Thr Thr Lys Gly Glu Asn Phe Thr 
                165                 170                 175     


Glu Thr Asp Val Lys Met Met Glu Arg Val Val Glu Gln Met Cys Val 
            180                 185                 190         


Thr Gln Tyr Gln Lys Glu Ser Gln Ala Tyr Tyr Asp Gly Arg Arg Ser 
        195                 200                 205             


<210>  3
<211>  209
<212>  PRT
<213>  Homo sapiens

<400>  3

Lys Lys Arg Pro Lys Pro Gly Gly Trp Asn Thr Gly Gly Ser Arg Tyr 
1               5                   10                  15      


Pro Gly Gln Gly Ser Pro Gly Gly Asn Arg Tyr Pro Pro Gln Gly Gly 
            20                  25                  30          


Gly Gly Trp Gly Gln Pro His Gly Gly Gly Trp Gly Gln Pro His Gly 
        35                  40                  45              


Gly Gly Trp Gly Gln Pro His Gly Gly Gly Trp Gly Gln Pro His Gly 
    50                  55                  60                  


Gly Gly Trp Gly Gln Gly Gly Gly Thr His Ser Gln Trp Asn Lys Pro 
65                  70                  75                  80  


Ser Lys Pro Lys Thr Asn Met Lys His Met Ala Gly Ala Ala Ala Ala 
                85                  90                  95      


Gly Ala Val Val Gly Gly Leu Gly Gly Tyr Met Leu Gly Ser Ala Met 
            100                 105                 110         


Ser Arg Pro Ile Ile His Phe Gly Ser Asp Tyr Glu Asp Arg Tyr Tyr 
        115                 120                 125             


Arg Glu Asn Met His Arg Tyr Pro Asn Gln Val Tyr Tyr Arg Pro Met 
    130                 135                 140                 


Asp Glu Tyr Ser Asn Gln Asn Asn Phe Val His Asp Cys Val Asn Ile 
145                 150                 155                 160 


Thr Ile Lys Gln His Thr Val Thr Thr Thr Thr Lys Gly Glu Asn Phe 
                165                 170                 175     


Thr Glu Thr Asp Val Lys Met Met Glu Arg Val Val Glu Gln Met Cys 
            180                 185                 190         


Ile Thr Gln Tyr Glu Arg Glu Ser Gln Ala Tyr Tyr Gln Arg Gly Ser 
        195                 200                 205             


Ser 
    


<210>  4
<211>  209
<212>  PRT
<213>  Homo sapiens

<400>  4

Lys Lys Arg Pro Lys Pro Gly Gly Trp Asn Thr Gly Gly Ser Arg Tyr 
1               5                   10                  15      


Pro Gly Gln Gly Ser Pro Gly Gly Asn Arg Tyr Pro Pro Gln Gly Gly 
            20                  25                  30          


Gly Gly Trp Gly Gln Pro His Gly Gly Gly Trp Gly Gln Pro His Gly 
        35                  40                  45              


Gly Gly Trp Gly Gln Pro His Gly Gly Gly Trp Gly Gln Pro His Gly 
    50                  55                  60                  


Gly Gly Trp Gly Gln Gly Gly Gly Thr His Ser Gln Trp Asn Lys Pro 
65                  70                  75                  80  


Ser Lys Pro Lys Thr Asn Met Lys His Met Ala Gly Ala Ala Ala Ala 
                85                  90                  95      


Gly Ala Val Val Gly Gly Leu Gly Gly Tyr Val Leu Gly Ser Ala Met 
            100                 105                 110         


Ser Arg Pro Ile Ile His Phe Gly Ser Asp Tyr Glu Asp Arg Tyr Tyr 
        115                 120                 125             


Arg Glu Asn Met His Arg Tyr Pro Asn Gln Val Tyr Tyr Arg Pro Met 
    130                 135                 140                 


Asp Glu Tyr Ser Asn Gln Asn Asn Phe Val His Asp Cys Val Asn Ile 
145                 150                 155                 160 


Thr Ile Lys Gln His Thr Val Thr Thr Thr Thr Lys Gly Glu Asn Phe 
                165                 170                 175     


Thr Glu Thr Asp Val Lys Met Met Glu Arg Val Val Glu Gln Met Cys 
            180                 185                 190         


Ile Thr Gln Tyr Glu Arg Glu Ser Gln Ala Tyr Tyr Gln Arg Gly Ser 
        195                 200                 205             


Ser 
    


<210>  5
<211>  218
<212>  PRT
<213>  Bos taurus

<400>  5

Lys Lys Arg Pro Lys Pro Gly Gly Gly Trp Asn Thr Gly Gly Ser Arg 
1               5                   10                  15      


Tyr Pro Gly Gln Gly Ser Pro Gly Gly Asn Arg Tyr Pro Pro Gln Gly 
            20                  25                  30          


Gly Gly Gly Trp Gly Gln Pro His Gly Gly Gly Trp Gly Gln Pro His 
        35                  40                  45              


Gly Gly Gly Trp Gly Gln Pro His Gly Gly Gly Trp Gly Gln Pro His 
    50                  55                  60                  


Gly Gly Gly Trp Gly Gln Pro His Gly Gly Gly Gly Trp Gly Gln Gly 
65                  70                  75                  80  


Gly Thr His Gly Gln Trp Asn Lys Pro Ser Lys Pro Lys Thr Asn Met 
                85                  90                  95      


Lys His Val Ala Gly Ala Ala Ala Ala Gly Ala Val Val Gly Gly Leu 
            100                 105                 110         


Gly Gly Tyr Met Leu Gly Ser Ala Met Ser Arg Pro Leu Ile His Phe 
        115                 120                 125             


Gly Ser Asp Tyr Glu Asp Arg Tyr Tyr Arg Glu Asn Met His Arg Tyr 
    130                 135                 140                 


Pro Asn Gln Val Tyr Tyr Arg Pro Val Asp Gln Tyr Ser Asn Gln Asn 
145                 150                 155                 160 


Asn Phe Val His Asp Cys Val Asn Ile Thr Val Lys Glu His Thr Val 
                165                 170                 175     


Thr Thr Thr Thr Lys Gly Glu Asn Phe Thr Glu Thr Asp Ile Lys Met 
            180                 185                 190         


Met Glu Arg Val Val Glu Gln Met Cys Ile Thr Gln Tyr Gln Arg Glu 
        195                 200                 205             


Ser Gln Ala Tyr Tyr Gln Arg Gly Ala Ser 
    210                 215             


<210>  6
<211>  209
<212>  PRT
<213>  Ovis aries

<400>  6

Lys Lys Arg Pro Lys Pro Gly Gly Gly Trp Asn Thr Gly Gly Ser Arg 
1               5                   10                  15      


Tyr Pro Gly Gln Gly Ser Pro Gly Gly Asn Arg Tyr Pro Pro Gln Gly 
            20                  25                  30          


Gly Gly Gly Trp Gly Gln Pro His Gly Gly Gly Trp Gly Gln Pro His 
        35                  40                  45              


Gly Gly Gly Trp Gly Gln Pro His Gly Gly Gly Trp Gly Gln Pro His 
    50                  55                  60                  


Gly Gly Gly Gly Trp Gly Gln Gly Gly Ser His Ser Gln Trp Asn Lys 
65                  70                  75                  80  


Pro Ser Lys Pro Lys Thr Asn Met Lys His Val Ala Gly Ala Ala Ala 
                85                  90                  95      


Ala Gly Ala Val Val Gly Gly Leu Gly Gly Tyr Met Leu Gly Ser Ala 
            100                 105                 110         


Met Ser Arg Pro Leu Ile His Phe Gly Asn Asp Tyr Glu Asp Arg Tyr 
        115                 120                 125             


Tyr Arg Glu Asn Met Tyr Arg Tyr Pro Asn Gln Val Tyr Tyr Arg Pro 
    130                 135                 140                 


Val Asp Gln Tyr Ser Asn Gln Asn Asn Phe Val His Asp Cys Val Asn 
145                 150                 155                 160 


Ile Thr Val Lys Gln His Thr Val Thr Thr Thr Thr Lys Gly Glu Asn 
                165                 170                 175     


Phe Thr Glu Thr Asp Ile Lys Ile Met Glu Arg Val Val Glu Gln Met 
            180                 185                 190         


Cys Ile Thr Gln Tyr Gln Arg Glu Ser Gln Ala Tyr Tyr Gln Arg Gly 
        195                 200                 205             


Ala 
    


<210>  7
<211>  210
<212>  PRT
<213>  Cervus unicolor

<400>  7

Lys Lys Arg Pro Lys Pro Gly Gly Gly Trp Asn Thr Gly Gly Ser Arg 
1               5                   10                  15      


Tyr Pro Gly Gln Gly Ser Pro Gly Gly Asn Arg Tyr Pro Pro Gln Gly 
            20                  25                  30          


Gly Gly Gly Trp Gly Gln Pro His Gly Gly Gly Trp Gly Gln Pro His 
        35                  40                  45              


Gly Gly Gly Trp Gly Gln Pro His Gly Gly Gly Trp Gly Gln Pro His 
    50                  55                  60                  


Gly Gly Gly Gly Trp Gly Gln Gly Gly Thr His Ser Gln Trp Asn Lys 
65                  70                  75                  80  


Pro Ser Lys Pro Lys Thr Asn Met Lys His Val Ala Gly Ala Ala Ala 
                85                  90                  95      


Ala Gly Ala Val Val Gly Gly Leu Gly Gly Tyr Met Leu Gly Ser Ala 
            100                 105                 110         


Met Ser Arg Pro Leu Ile His Phe Gly Asn Asp Tyr Glu Asp Arg Tyr 
        115                 120                 125             


Tyr Arg Glu Asn Met Tyr Arg Tyr Pro Asn Gln Val Tyr Tyr Arg Pro 
    130                 135                 140                 


Val Asp Gln Tyr Asn Asn Gln Asn Thr Phe Val His Asp Cys Val Asn 
145                 150                 155                 160 


Ile Thr Val Lys Gln His Thr Val Thr Thr Thr Thr Lys Gly Glu Asn 
                165                 170                 175     


Phe Thr Glu Thr Asp Ile Lys Met Met Glu Arg Val Val Glu Gln Met 
            180                 185                 190         


Cys Ile Thr Gln Tyr Gln Arg Glu Ser Gln Ala Tyr Tyr Gln Arg Gly 
        195                 200                 205             


Ala Ser 
    210 


<210>  8
<211>  240
<212>  PRT
<213>  Mesocricetus auratus

<400>  8

Met Trp Thr Asp Val Gly Leu Cys Lys Lys Arg Pro Lys Pro Gly Gly 
1               5                   10                  15      


Trp Asn Thr Gly Gly Ser Arg Tyr Pro Gly Gln Gly Ser Pro Gly Gly 
            20                  25                  30          


Asn Arg Tyr Pro Pro Gln Gly Gly Gly Thr Trp Gly Gln Pro His Gly 
        35                  40                  45              


Gly Gly Trp Gly Gln Pro His Gly Gly Gly Trp Gly Gln Pro His Gly 
    50                  55                  60                  


Gly Gly Trp Gly Gln Pro His Gly Gly Gly Trp Gly Gln Gly Gly Gly 
65                  70                  75                  80  


Thr His Asn Gln Trp Asn Lys Pro Ser Lys Pro Lys Thr Asn Met Lys 
                85                  90                  95      


His Met Ala Gly Ala Ala Ala Ala Gly Ala Val Val Gly Gly Leu Gly 
            100                 105                 110         


Gly Tyr Met Leu Gly Ser Ala Met Ser Arg Pro Met Met His Phe Gly 
        115                 120                 125             


Asn Asp Trp Glu Asp Arg Tyr Tyr Arg Glu Asn Met Asn Arg Tyr Pro 
    130                 135                 140                 


Asn Gln Val Tyr Tyr Arg Pro Val Asp Gln Tyr Asn Asn Gln Asn Asn 
145                 150                 155                 160 


Phe Val His Asp Cys Val Asn Ile Thr Ile Lys Gln His Thr Val Thr 
                165                 170                 175     


Thr Thr Thr Lys Gly Glu Asn Phe Thr Glu Thr Asp Ile Lys Ile Met 
            180                 185                 190         


Glu Arg Val Val Glu Gln Met Cys Thr Thr Gln Tyr Gln Lys Glu Ser 
        195                 200                 205             


Gln Ala Tyr Tyr Asp Gly Arg Arg Ser Ser Ala Val Leu Phe Ser Ser 
    210                 215                 220                 


Pro Pro Val Ile Leu Leu Ile Ser Phe Leu Ile Phe Leu Met Val Gly 
225                 230                 235                 240 


<210>  9
<211>  254
<212>  PRT
<213>  Mus musculus

<400>  9

Met Ala Asn Leu Gly Tyr Trp Leu Leu Ala Leu Phe Val Thr Met Trp 
1               5                   10                  15      


Thr Asp Val Gly Leu Cys Lys Lys Arg Pro Lys Pro Gly Gly Trp Asn 
            20                  25                  30          


Thr Gly Gly Ser Arg Tyr Pro Gly Gln Gly Ser Pro Gly Gly Asn Arg 
        35                  40                  45              


Tyr Pro Pro Gln Gly Gly Thr Trp Gly Gln Pro His Gly Gly Gly Trp 
    50                  55                  60                  


Gly Gln Pro His Gly Gly Ser Trp Gly Gln Pro His Gly Gly Ser Trp 
65                  70                  75                  80  


Gly Gln Pro His Gly Gly Gly Trp Gly Gln Gly Gly Gly Thr His Asn 
                85                  90                  95      


Gln Trp Asn Lys Pro Ser Lys Pro Lys Thr Asn Leu Lys His Val Ala 
            100                 105                 110         


Gly Ala Ala Ala Ala Gly Ala Val Val Gly Gly Leu Gly Gly Tyr Met 
        115                 120                 125             


Leu Gly Ser Ala Met Ser Arg Pro Met Ile His Phe Gly Asn Asp Trp 
    130                 135                 140                 


Glu Asp Arg Tyr Tyr Arg Glu Asn Met Tyr Arg Tyr Pro Asn Gln Val 
145                 150                 155                 160 


Tyr Tyr Arg Pro Val Asp Gln Tyr Ser Asn Gln Asn Asn Phe Val His 
                165                 170                 175     


Asp Cys Val Asn Ile Thr Ile Lys Gln His Thr Val Thr Thr Thr Thr 
            180                 185                 190         


Lys Gly Glu Asn Phe Thr Glu Thr Asp Val Lys Met Met Glu Arg Val 
        195                 200                 205             


Val Glu Gln Met Cys Val Thr Gln Tyr Gln Lys Glu Ser Gln Ala Tyr 
    210                 215                 220                 


Tyr Asp Gly Arg Arg Ser Ser Ser Thr Val Leu Phe Ser Ser Pro Pro 
225                 230                 235                 240 


Val Ile Leu Leu Ile Ser Phe Leu Ile Phe Leu Ile Val Gly 
                245                 250                 


<210>  10
<211>  253
<212>  PRT
<213>  Homo sapiens

<400>  10

Met Ala Asn Leu Gly Cys Trp Met Leu Val Leu Phe Val Ala Thr Trp 
1               5                   10                  15      


Ser Asp Leu Gly Leu Cys Lys Lys Arg Pro Lys Pro Gly Gly Trp Asn 
            20                  25                  30          


Thr Gly Gly Ser Arg Tyr Pro Gly Gln Gly Ser Pro Gly Gly Asn Arg 
        35                  40                  45              


Tyr Pro Pro Gln Gly Gly Gly Gly Trp Gly Gln Pro His Gly Gly Gly 
    50                  55                  60                  


Trp Gly Gln Pro His Gly Gly Gly Trp Gly Gln Pro His Gly Gly Gly 
65                  70                  75                  80  


Trp Gly Gln Pro His Gly Gly Gly Trp Gly Gln Gly Gly Gly Thr His 
                85                  90                  95      


Ser Gln Trp Asn Lys Pro Ser Lys Pro Lys Thr Asn Met Lys His Met 
            100                 105                 110         


Ala Gly Ala Ala Ala Ala Gly Ala Val Val Gly Gly Leu Gly Gly Tyr 
        115                 120                 125             


Met Leu Gly Ser Ala Met Ser Arg Pro Ile Ile His Phe Gly Ser Asp 
    130                 135                 140                 


Tyr Glu Asp Arg Tyr Tyr Arg Glu Asn Met His Arg Tyr Pro Asn Gln 
145                 150                 155                 160 


Val Tyr Tyr Arg Pro Met Asp Glu Tyr Ser Asn Gln Asn Asn Phe Val 
                165                 170                 175     


His Asp Cys Val Asn Ile Thr Ile Lys Gln His Thr Val Thr Thr Thr 
            180                 185                 190         


Thr Lys Gly Glu Asn Phe Thr Glu Thr Asp Val Lys Met Met Glu Arg 
        195                 200                 205             


Val Val Glu Gln Met Cys Ile Thr Gln Tyr Glu Arg Glu Ser Gln Ala 
    210                 215                 220                 


Tyr Tyr Gln Arg Gly Ser Ser Met Val Leu Phe Ser Ser Pro Pro Val 
225                 230                 235                 240 


Ile Leu Leu Ile Ser Phe Leu Ile Phe Leu Ile Val Gly 
                245                 250             


<210>  11
<211>  130
<212>  PRT
<213>  Homo sapiens

<400>  11

Met Ala Asn Leu Gly Cys Trp Met Leu Val Leu Phe Val Ala Thr Trp 
1               5                   10                  15      


Ser Asp Leu Gly Leu Cys Lys Lys Arg Pro Lys Pro Gly Gly Trp Asn 
            20                  25                  30          


Thr Gly Gly Ser Arg Tyr Pro Gly Gln Gly Ser Pro Gly Gly Asn Arg 
        35                  40                  45              


Tyr Pro Pro Gln Gly Gly Gly Gly Trp Gly Gln Pro His Gly Gly Gly 
    50                  55                  60                  


Trp Gly Gln Pro His Gly Gly Gly Trp Gly Gln Pro His Gly Gly Gly 
65                  70                  75                  80  


Trp Gly Gln Pro His Gly Gly Gly Trp Gly Gln Gly Gly Gly Thr His 
                85                  90                  95      


Ser Gln Trp Asn Lys Pro Ser Lys Pro Lys Thr Asn Met Lys His Met 
            100                 105                 110         


Ala Gly Ala Ala Ala Ala Gly Ala Val Val Gly Gly Leu Gly Gly Tyr 
        115                 120                 125             


Val Leu 
    130 


