                         SEQUENCE LISTING

<110>  The United States Army Medical Research and Materiel 
       Command
       Pillips, Christopher
       Ruff, Albert
       Dillman, James
 
<120>  UNIQUE CALIBRATOR POLYNUCLEOTIDES AND METHODS OF USING IN 
       QUANTITATIVE NUCLEIC ACID ASSAYS

<130>  034047.050WO1

<150>  US 60/949,677
<151>  2007-07-13

<160>  14    

<170>  PatentIn version 3.5

<210>  1
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PVS target sequence

<400>  1
ctgtcgcttc ggctactacc cggtg                                             25


<210>  2
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CLS target sequence

<400>  2
agatgcgttc cgctttacaa ctaacgaaca                                        30


<210>  3
<211>  80
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of calibrator polynucleotide

<400>  3
ggagtaattc ccgccgaaac agggttttcc tgtcgcttcg gctactaccc ggtggaaaca       60

actgaagctc ccgagaaccg                                                   80


<210>  4
<211>  95
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of calibrator polynucleotide

<400>  4
gaactcccgg aattgatgga attatctggt agatgcgttc cgctttacaa ctaacgaaca       60

agggctacaa gtacattcga aagaagaacg gtaaa                                  95


<210>  5
<211>  141
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of calibrator polynucleotide


<220>
<221>  misc_feature
<222>  (41)..(41)
<223>  n is a, c, g, or t

<400>  5
ggagtaattc ccgccgaaac agggttttca cccttccttt nttcgggtgt ccttcctcgc       60

gcccgcagga ccacccctcg cccctttgcg ctgtcgcttc ggctactacc cggtggaaac      120

aactgaagct cccgagaacc g                                                141


<210>  6
<211>  131
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of calibrator polynucleotide

<400>  6
gaactcccgg aattgatgga attatctggt catcgtcggc agataagatg cgttccgctt       60

tacaactaac gaacaagggc taccgtgcaa aaccattaac acgaaagtac attcgaaaga      120

agaacggtaa a                                                           131


<210>  7
<211>  358
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of calibrator polynucleotide


<220>
<221>  misc_feature
<222>  (112)..(112)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (147)..(147)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (193)..(193)
<223>  n is a, c, g, or t

<400>  7
cggaactaaa ctcgtggttc ctgtggttca cacctgacct cctgagcaga aaagaaaaaa       60

gaattgcggc tcggaggagc gcttcagggc atccccgggg aaacctggag cnaactggca      120

ataaggcggt gggaagtggc caacggncga caggagtaat tcccgccgaa acagggtttt      180

cacccttcct ttnttcgggt gtccttcctc gcgcccgcag gaccacccct cgcccctttg      240

cgctgtcgct tcggctacta cccggtggaa acaactgaag ctcccgagaa ccgctttttc      300

tctatcttcc ttgcttcggg gcgagggtgt ttagcccttg gaaccgcagt tggttcct        358


<210>  8
<211>  623
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of calibrator polynucleotide

<400>  8
aagcattgat tggaaaagag ttaaagaagt tgttaataac cttcagtctc gaattgcaag       60

tgcagctaag aacggaaaat ggataaccgt gaacaaactc tcccgtcttc tgacccggtc      120

cttatatgcc aaactacttt cagttcgtaa agtaaccact aacaagggaa gccgaactcc      180

cggaattgat ggaattatct ggtcatcgtc ggcagataag atgcgttccg ctttacaact      240

aacgaacaag ggctaccgtg caaaaccatt aacacgaaag tacattcgaa agaagaacgg      300

taaactacga cctcttagca taccaactat gtatgacaga gcaatgcaaa ccctgcactc      360

tctggtgcta ggtccaatcg aatctgctat aggtgacaag acttcgtttg ggtttaaacc      420

ttaccgctca actaaagatg cttacgccta ccttcacatc tgtttaagca agaaaattgc      480

tcctgaatgg attgtcgaag gtgatattaa agcctgcttt gatgaaatca accacacttg      540

gatacttgac aacatcccta tggataaacg aatccttaag gagtttctaa aagccggata      600

tgtcgagaat tatcatctgt ttc                                              623


<210>  9
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer used to amplify SEQ ID NO:3

<400>  9
cgtagcggta ccggagtaat tcccgccgaa aca                                    33


<210>  10
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer used to amplify SEQ ID NO:3

<400>  10
cgccgggtcg accggttctc gggagcttca gtt                                    33


<210>  11
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer used to amplify SEQ ID NO:4

<400>  11
cgtagcggta ccgaactccc ggaattgatg gaat                                   34


<210>  12
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer used to amplify SEQ ID NO:4

<400>  12
cgccgggtcg actttaccgt tcttctttcg aatgtacttg                             40


<210>  13
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer anneals to nucleotides 1-18 on the parent plasmid 
       (pTNT from Promega, GenBank accession #AF479322)

<400>  13
taaggctaga gtacttaa                                                     18


<210>  14
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer anneals to nucleotides 195-213 on the parent 
       plasmid (pTNT from Promega, GenBank accession #AF479322)

<400>  14
ggatccaaaa aacccctc                                                     18


