
                                SEQUENCE LISTING

<110> Chou, Howard H.
      Keasling, Jay D.

<120> HOST CELLS AND METHODS FOR PRODUCING 3
  METHYL-2-BUTEN-1-OL, 3-METHYL-3-BUTEN-1-OL, AND 3-METHYL
  BUTAN-1-OL

<130> 014939-006810PC

<150> 60/947,285          
<151> 2007-06-29  

<160> 69

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 4
<212> PRT
<213> Artificial Sequence

<220> 
<223> conserved phosphatase enzyme sequence

<400> 1
Arg His Gly Glu
 1              


<210> 2
<211> 5
<212> PRT
<213> Arificial Sequence

<400> 2
Arg His Gly Glu Thr
 1               5  


<210> 3
<211> 5
<212> PRT
<213> Artificial Sequence

<220> 
<223> conserved phosphatase enzyme sequence

<400> 3
Arg His Gly Glu Ser
 1               5  


<210> 4
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<223> conserved phosphatase enzyme sequence

<400> 4
Arg His Gly Glu Thr Trp Asn
 1               5          


<210> 5
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<223> conserved phosphatase enzyme sequence

<400> 5
Arg His Gly Glu Ser Trp Asn
 1               5          


<210> 6
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<223> conserved phosphatase enzyme sequence

<400> 6
Arg His Gly Glu Thr Gly Asn
 1               5          


<210> 7
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<223> conserved phosphatase enzyme sequence

<400> 7
Arg His Gly Glu Ser Gly Asn
 1               5          


<210> 8
<211> 8
<212> PRT
<213> Artificial Sequence

<220> 
<223> conserved phosphatase enzyme sequence

<221> VARIANT        
<222> (4)...(7)
<223> Xaa = any amino acid

<400> 8
Arg His Gly Xaa Xaa Xaa Xaa Asn
 1               5              


<210> 9
<211> 143
<212> PRT
<213> Artificial Sequence

<220> 
<223> conserved phosphatase enzyme sequence

<221> VARIANT        
<222> (5)...(7)
<223> Xaa = any amino acid

<221> VARIANT        
<222> (9)...(50)
<223> Xaa = any amino acid

<221> VARIANT        
<222> (52)...(74)
<223> Xaa = any amino acid

<221> VARIANT        
<222> (76)...(142)
<223> Xaa = any amino acid; Xaa 132 through Xaa 142 can
      be present or absent

<400> 9
Arg His Gly Glu Xaa Xaa Xaa Asn Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa
 1               5                  10                  15      
Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa
            20                  25                  30          
Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa
        35                  40                  45              
Xaa Xaa Arg Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa
    50                  55                  60                  
Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Glu Xaa Xaa Xaa Xaa Xaa
65                  70                  75                  80  
Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa
                85                  90                  95      
Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa
            100                 105                 110         
Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa
        115                 120                 125             
Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa His
    130                 135                 140             


<210> 10
<211> 15
<212> PRT
<213> Artificial Sequence

<220> 
<223> conserved phosphatase enzyme sequence

<221> VARIANT        
<222> (5)...(7)
<223> Xaa =  any amino acid

<221> VARIANT        
<222> (9)...(13)
<223> Xaa = any amino acid

<400> 10
Arg His Gly Glu Xaa Xaa Xaa Asn Xaa Xaa Xaa Xaa Xaa Gln Gly
 1               5                  10                  15  


<210> 11
<211> 25
<212> PRT
<213> Artificial Sequence

<220> 
<223> conserved phosphatase enzyme sequence

<221> VARIANT        
<222> (4)...(7)
<223> Xaa = any amino acid

<221> VARIANT        
<222> (9)...(17)
<223> Xaa = any amino acid; Xaa 16 through Xaa 17 can be
      present or absent

<221> VARIANT        
<222> (19)...(20)
<223> Xaa = any amino acid

<221> VARIANT        
<222> (22)...(24)
<223> Xaa = any amino acid

<400> 11
Arg His Gly Xaa Xaa Xaa Xaa Asn Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa
 1               5                  10                  15      
Xaa Asp Xaa Xaa Leu Xaa Xaa Xaa Gly
            20                  25  


<210> 12
<211> 24
<212> PRT
<213> Artificial Sequence

<220> 
<223> consensus Nudix box motif

<221> VARIANT        
<222> (2)...(6)
<223> Xaa = any amino acid

<221> VARIANT        
<222> (8)...(12)
<223> Xaa = any amino acid

<221> VARIANT        
<222> (13)...(13)
<223> Xaa = any aliphatic, hydrophobic amino acid

<221> VARIANT        
<222> (15)...(15)
<223> Xaa = any amino acid

<221> VARIANT        
<222> (18)...(19)
<223> Xaa = any amino acid

<221> VARIANT        
<222> (22)...(22)
<223> Xaa = any amino acid

<221> VARIANT        
<222> (24)...(24)
<223> Xaa = any aliphatic, hydrophobic amino acid

<400> 12
Gly Xaa Xaa Xaa Xaa Xaa Glu Xaa Xaa Xaa Xaa Xaa Xaa Ala Xaa Arg
 1               5                  10                  15      
Glu Xaa Xaa Glu Glu Xaa Gly Xaa
            20                  


<210> 13
<211> 22
<212> PRT
<213> Artificial Sequence

<220> 
<223> conserved Nudix box motif

<221> VARIANT        
<222> (2)...(6)
<223> Xaa = any amino acid

<221> VARIANT        
<222> (8)...(13)
<223> Xaa = any amino acid

<221> VARIANT        
<222> (16)...(17)
<223> Xaa = any amino acid

<221> VARIANT        
<222> (20)...(20)
<223> Xaa = any amino acid

<221> VARIANT        
<222> (22)...(22)
<223> Xaa = any aliphatic, hyrodphobic amino acid

<400> 13
Gly Xaa Xaa Xaa Xaa Xaa Glu Xaa Xaa Xaa Xaa Xaa Xaa Arg Glu Xaa
 1               5                  10                  15      
Xaa Glu Glu Xaa Gly Xaa
            20          


<210> 14
<211> 11
<212> PRT
<213> Artificial Sequence

<220> 
<223> conserved OYE family consensus sequence

<221> VARIANT        
<222> (3)...(3)
<223> Xaa = any amino acid

<400> 14
Ser Asn Xaa Arg Thr Asp Glu Tyr Gly Gly Ser
 1               5                  10      


<210> 15
<211> 11
<212> PRT
<213> Artificial Sequence

<220> 
<223> conserved OYE family consensus sequence

<221> VARIANT        
<222> (3)...(3)
<223> Xaa = any amino acid

<400> 15
Ser Asn Xaa Arg Thr Asp Glu Tyr Gly Gly Thr
 1               5                  10      


<210> 16
<211> 15
<212> PRT
<213> Artificial Sequence

<220> 
<223> zinc-dependent alcohol dehydrogenase consensus
      sequence

<221> VARIANT        
<222> (4)...(5)
<223> Xaa = any amino acid

<221> VARIANT        
<222> (7)...(11)
<223> Xaa = any amino acid

<221> VARIANT        
<222> (13)...(14)
<223> Xaa = any amino acid

<400> 16
Gly His Glu Xaa Xaa Gly Xaa Xaa Xaa Xaa Xaa Gly Xaa Xaa Ile
 1               5                  10                  15  


<210> 17
<211> 15
<212> PRT
<213> Artificial Sequence

<220> 
<223> zinc-dependent alcohol dehydrogenase consensus
      sequence

<221> VARIANT        
<222> (4)...(5)
<223> Xaa =  any amino acid

<221> VARIANT        
<222> (7)...(11)
<223> Xaa = any amino acid

<221> VARIANT        
<222> (13)...(14)
<223> Xaa =  any amino acid

<400> 17
Gly His Glu Xaa Xaa Gly Xaa Xaa Xaa Xaa Xaa Gly Xaa Xaa Val
 1               5                  10                  15  


<210> 18
<211> 15
<212> PRT
<213> Artificial Sequence

<220> 
<223> zinc-dependent alcohol dehydrogenase consensus
      sequence

<221> VARIANT        
<222> (4)...(5)
<223> Xaa = any amino acid

<221> VARIANT        
<222> (7)...(11)
<223> Xaa = any amino acid

<221> VARIANT        
<222> (13)...(14)
<223> Xaa = any amino acid

<400> 18
Gly His Glu Xaa Xaa Gly Xaa Xaa Xaa Xaa Xaa Gly Xaa Xaa Ala
 1               5                  10                  15  


<210> 19
<211> 15
<212> PRT
<213> Artificial Sequence

<220> 
<223> zinc-dependent alcohol dehydrogenase consensus
      sequence

<221> VARIANT        
<222> (4)...(5)
<223> Xaa = any amino acid

<221> VARIANT        
<222> (7)...(11)
<223> Xaa = any amino acid

<221> VARIANT        
<222> (13)...(14)
<223> Xaa = any amino acid

<400> 19
Gly His Glu Xaa Xaa Gly Xaa Xaa Xaa Xaa Xaa Gly Xaa Xaa Cys
 1               5                  10                  15  


<210> 20
<211> 15
<212> PRT
<213> Artificial Sequence

<220> 
<223> zinc-dependent alcohol dehydrogenase consensus
      sequence

<221> VARIANT        
<222> (4)...(5)
<223> Xaa = any amino acid

<221> VARIANT        
<222> (7)...(11)
<223> Xaa = any amino acid

<221> VARIANT        
<222> (13)...(14)
<223> Xaa = any amino acid

<400> 20
Gly His Glu Xaa Xaa Gly Xaa Xaa Xaa Xaa Xaa Gly Xaa Xaa Ser
 1               5                  10                  15  


<210> 21
<211> 15
<212> PRT
<213> Artificial Sequence

<220> 
<223> zinc-dependent alcohol dehydrogenase consensus
      sequence

<221> VARIANT        
<222> (4)...(5)
<223> Xaa = any amino acid

<221> VARIANT        
<222> (7)...(11)
<223> Xaa = any amino acid

<221> VARIANT        
<222> (13)...(14)
<223> Xaa = any amino acid

<400> 21
Gly His Glu Xaa Xaa Gly Xaa Xaa Xaa Xaa Xaa Ala Xaa Xaa Ile
 1               5                  10                  15  


<210> 22
<211> 15
<212> PRT
<213> Artificial Sequence

<220> 
<223> zinc-dependent alcohol dehydrogenase consensus
      sequence

<221> VARIANT        
<222> (4)...(5)
<223> Xaa = any amino acid

<221> VARIANT        
<222> (7)...(11)
<223> Xaa = any amino acid

<221> VARIANT        
<222> (13)...(14)
<223> Xaa = any amino acid

<400> 22
Gly His Glu Xaa Xaa Gly Xaa Xaa Xaa Xaa Xaa Ala Xaa Xaa Val
 1               5                  10                  15  


<210> 23
<211> 15
<212> PRT
<213> Artificial Sequence

<220> 
<223> zinc-dependent alcohol dehydrogenase consensus
      sequence

<221> VARIANT        
<222> (4)...(5)
<223> Xaa = any amino acid

<221> VARIANT        
<222> (7)...(11)
<223> Xaa = any amino acid

<221> VARIANT        
<222> (13)...(14)
<223> Xaa = any amino acid

<400> 23
Gly His Glu Xaa Xaa Gly Xaa Xaa Xaa Xaa Xaa Ala Xaa Xaa Ala
 1               5                  10                  15  


<210> 24
<211> 15
<212> PRT
<213> Artificial Sequence

<220> 
<223> zinc-dependent alcohol dehydrogenase consensus
      sequence

<221> VARIANT        
<222> (4)...(5)
<223> Xaa = any amino acid

<221> VARIANT        
<222> (7)...(11)
<223> Xaa = any amino acid

<221> VARIANT        
<222> (13)...(14)
<223> Xaa = any amino acid

<400> 24
Gly His Glu Xaa Xaa Gly Xaa Xaa Xaa Xaa Xaa Ala Xaa Xaa Cys
 1               5                  10                  15  


<210> 25
<211> 15
<212> PRT
<213> Artificial Sequence

<220> 
<223> zinc-dependent alcohol dehydrogenase consensus
      sequence

<221> VARIANT        
<222> (4)...(5)
<223> Xaa = any amino acid

<221> VARIANT        
<222> (7)...(11)
<223> Xaa = any amino acid

<221> VARIANT        
<222> (13)...(14)
<223> Xaa = any amino acid

<400> 25
Gly His Glu Xaa Xaa Gly Xaa Xaa Xaa Xaa Xaa Ala Xaa Xaa Ser
 1               5                  10                  15  


<210> 26
<211> 34
<212> DNA
<213> Artificial Sequence

<220> 
<223> YHR179w primer 1

<400> 26
aaccgtcgac atgccatttg ttaaggactt taag                             34

<210> 27
<211> 40
<212> DNA
<213> Artificial Sequence

<220> 
<223> YHR179w primer 2

<400> 27
taacgctagc ttaatttttg tcccaaccga gttttagagc                       40

<210> 28
<211> 35
<212> DNA
<213> Artificial Sequence

<220> 
<223> YPL171c primer 1

<400> 28
aaccgtcgac atgccatttg taaaaggttt tgagc                            35

<210> 29
<211> 37
<212> DNA
<213> Artificial Sequence

<220> 
<223> YPL171c primer 2

<400> 29
taacgctagc tcagttcttg ttccaaccta aatctac                          37

<210> 30
<211> 33
<212> DNA
<213> Artificial Sequence

<220> 
<223> YOR120w primer 1

<400> 30
aaccggatcc atgcctgcta ctttacatga ttc                              33

<210> 31
<211> 33
<212> DNA
<213> Artificial Sequence

<220> 
<223> YOR120w primer 2

<400> 31
taacctcgag ttacttgaat acttcgaaag gag                              33

<210> 32
<211> 30
<212> DNA
<213> Artificial Sequence

<220> 
<223> YDR368w primer 1

<400> 32
aaccgtcgac atgcctgcta cgttaaagaa                                  30

<210> 33
<211> 29
<212> DNA
<213> Artificial Sequence

<220> 
<223> YDR368w primer 2

<400> 33
taacctcgag tcattggaaa attgggaag                                   29

<210> 34
<211> 30
<212> DNA
<213> Artificial Sequence

<220> 
<223> YBR149w primer 1

<400> 34
aaccggatcc atgtcttctt cagtagcctc                                  30

<210> 35
<211> 32
<212> DNA
<213> Artificial Sequence

<220> 
<223> YBR149w primer 2

<400> 35
taacgctagc ttaatacttt aaattgtcca ag                               32

<210> 36
<211> 32
<212> DNA
<213> Artificial Sequence

<220> 
<223> YHR104w primer 1

<400> 36
aaccggatcc atgtcttcac tggttactct ta                               32

<210> 37
<211> 29
<212> DNA
<213> Artificial Sequence

<220> 
<223> YHR104w primer 2

<400> 37
taacctcgag tcaggcaaaa gtggggaat                                   29

<210> 38
<211> 31
<212> DNA
<213> Artificial Sequence

<220> 
<223> YDL124w primer 1

<400> 38
aaccggatcc atgtcatttc accaacagtt c                                31

<210> 39
<211> 29
<212> DNA
<213> Artificial Sequence

<220> 
<223> YDL124w primer 2

<400> 39
taacctcgag ttatactttt tgagcagcg                                   29

<210> 40
<211> 32
<212> DNA
<213> Artificial Sequence

<220> 
<223> YJR096w primer 1

<400> 40
aaccggatcc atggttccta agttttacaa ac                               32

<210> 41
<211> 31
<212> DNA
<213> Artificial Sequence

<220> 
<223> YJR096w primer 2

<400> 41
taacctcgag ttatggcgcg tctgtgcatt c                                31

<210> 42
<211> 32
<212> DNA
<213> Artificial Sequence

<220> 
<223> YIR036c primer 1

<400> 42
aaccggatcc atgggcaagg ttattttgat ta                               32

<210> 43
<211> 33
<212> DNA
<213> Artificial Sequence

<220> 
<223> YIR036c primer 2

<400> 43
taacgctagc ctagccctgc accggcccca gtc                              33

<210> 44
<211> 32
<212> DNA
<213> Artificial Sequence

<220> 
<223> YMR226c primer 1

<400> 44
aaccggatcc atgtcccaag gtagaaaagc tg                               32

<210> 45
<211> 35
<212> DNA
<213> Artificial Sequence

<220> 
<223> YMR226c primer 2

<400> 45
taacgctagc ttatccacgg aagatatgat gaggt                            35

<210> 46
<211> 32
<212> DNA
<213> Artificial Sequence

<220> 
<223> YOL151w primer 1

<400> 46
aaccggatcc atgtcagttt tcgtttcagg tg                               32

<210> 47
<211> 36
<212> DNA
<213> Artificial Sequence

<220> 
<223> YOL151w primer 2

<400> 47
taacctcgag ttatattctg ccctcaaatt ttaaaa                           36

<210> 48
<211> 34
<212> DNA
<213> Artificial Sequence

<220> 
<223> YGL157w primer 1

<400> 48
aaccggatcc atgactactg ataccactgt tttc                             34

<210> 49
<211> 28
<212> DNA
<213> Artificial Sequence

<220> 
<223> YGL157w primer 2

<400> 49
taacctcgag ttaggcttca ttttgaac                                    28

<210> 50
<211> 29
<212> DNA
<213> Artificial Sequence

<220> 
<223> YDR541c primer 1

<400> 50
aaccggatcc atgtctaata cagttctag                                   29

<210> 51
<211> 32
<212> DNA
<213> Artificial Sequence

<220> 
<223> YDR541c primer 2

<400> 51
aaccggatcc tcataatctg ttctccttct tc                               32

<210> 52
<211> 32
<212> DNA
<213> Artificial Sequence

<220> 
<223> YGL039w primer 1

<400> 52
aaccgtcgac atgactactg aaaaaaccgt tg                               32

<210> 53
<211> 42
<212> DNA
<213> Artificial Sequence

<220> 
<223> YGL039w primer 2

<400> 53
taacgctagc ttagctttta ctttgaactt ctagtaattg cg                    42

<210> 54
<211> 31
<212> DNA
<213> Artificial Sequence

<220> 
<223> YAL060w primer 1

<400> 54
aaccggatcc atgagagctt tggcatattt c                                31

<210> 55
<211> 29
<212> DNA
<213> Artificial Sequence

<220> 
<223> YAL060w primer 2

<400> 55
taacctcgag ttacttcatt tcaccgtga                                   29

<210> 56
<211> 30
<212> DNA
<213> Artificial Sequence

<220> 
<223> YLR070c primer 1

<400> 56
aaccggatcc atgactgact taactacaca                                  30

<210> 57
<211> 32
<212> DNA
<213> Artificial Sequence

<220> 
<223> YLR070c primer 2

<400> 57
taacctcgag tcattccggg ccctcaatga tc                               32

<210> 58
<211> 28
<212> DNA
<213> Artificial Sequence

<220> 
<223> YGL185c primer 1

<400> 58
aaccggatcc atgtgcgatt ctcctgca                                    28

<210> 59
<211> 33
<212> DNA
<213> Artificial Sequence

<220> 
<223> YGL185c primer 2

<400> 59
taacctcgag tcaaactaca cgggagaaat gct                              33

<210> 60
<211> 34
<212> DNA
<213> Artificial Sequence

<220> 
<223> YNL274c primer 1

<400> 60
aaccggatcc atgagtaaga aaccaattgt tttg                             34

<210> 61
<211> 31
<212> DNA
<213> Artificial Sequence

<220> 
<223> YNL274c primer 2

<400> 61
taacctcgag tcaaactaat ggcttagatt c                                31

<210> 62
<211> 35
<212> DNA
<213> Artificial Sequence

<220> 
<223> YPL113c primer 1

<400> 62
aaccggatcc atgattactt caattgacat agcag                            35

<210> 63
<211> 36
<212> DNA
<213> Artificial Sequence

<220> 
<223> YPL113c primer 2

<400> 63
taacgctagc tcagttgagc acatacttac catcac                           36

<210> 64
<211> 30
<212> DNA
<213> Artificial Sequence

<220> 
<223> YCR107w primer 1

<400> 64
aaccggatcc atgattgggt ccgcgtccga                                  30

<210> 65
<211> 28
<212> DNA
<213> Artificial Sequence

<220> 
<223> YCR107w primer 2

<400> 65
taacctcgag ctaaacatta ttcgtacc                                    28

<210> 66
<211> 32
<212> DNA
<213> Artificial Sequence

<220> 
<223> YNL331c primer 1

<400> 66
aaccggatcc atgactgact tgtttaaacc tc                               32

<210> 67
<211> 32
<212> DNA
<213> Artificial Sequence

<220> 
<223> YNL331c primer 2

<400> 67
taacctcgag ctaattgtca aaagctatcc tg                               32

<210> 68
<211> 34
<212> DNA
<213> Artificial Sequence

<220> 
<223> YPL275w primer 1

<400> 68
aaccggatcc atggtggtca tcaataagca atta                             34

<210> 69
<211> 36
<212> DNA
<213> Artificial Sequence

<220> 
<223> YPL275w primer 2

<400> 69
taacctcgag ttatttcttc tgtccataag ctctgg                           36
