
                                SEQUENCE LISTING

<110> Merck & Co., Inc.
      Garcia, Maria L.
      Kohler, Martin G.
      Weinglass, Adam

<120> USE OF MDCK CELLS IN THE EVALUATION OF
  CHOLESTEROL MODULATORS
  

<130> 22108

<150> 60/937,798          
<151> 2007-06-28  

<160> 5

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 4
<212> PRT
<213> Artificial Sequence

<220> 
<223> Protein Motif

<400> 1
Tyr Gln Arg Leu
 1              


<210> 2
<211> 30
<212> DNA
<213> Artificial Sequence

<220> 
<223> PCR PRIMER dNL1-s

<400> 2
ctgcacaggg atggcggaca ctggcctgag                                  30

<210> 3
<211> 31
<212> DNA
<213> Artificial Sequence

<220> 
<223> PCR PRIMER dNL1-as

<400> 3
ctccggcttc atcagaggtc cggtccactg c                                31

<210> 4
<211> 3978
<212> DNA
<213> Canine NPC1L1

<400> 4
atggcggaca ctggcctgag gggctggctg ctatgggcac tgctcctgca tgtggcccag 60
agtgagctgt acacacccat ccaccagcct ggctactgcg ctttctacga cgagtgtggg 120
aagaacccag agctgtctgg gggactggcg cctctgtcta atgtgtcctg cctgtccaac 180
acgcccgccc cccgtgtcac tggtgagcac ctgaccctcc tacagcgcat ctgcccccgc 240
ctctacacgg gcaccaccac ctatgcctgc tgctccccca agcagctgct gtccctggag 300
acgagcctgg cggtcaccaa ggccctcctc acccgctgcc ccacctgctc cgacaacttt 360
gtgaacctgc actgccaaaa cacctgcagc cccaaccaaa gtctcttcat caacgtgacc 420
cgcgtggctg ggggcggggg tggccggccc caggctgtgg tggcctatga ggccttctac 480
caggacacct ttgcccagca gacctacgac tcttgcagcc gggtgcgcat ccctgcggct 540
gccacgctgg ccgtgggcac catgtgtggc gtttatggct ccaccctctg caatgctcag 600
cgctggctca atttccaggg ggacacttcg aatggcctgg ctcccctaga catcaccttc 660
cacctgatgg agcccggcca ggccctaggg agtgggatgc aggctctgac cggggagatc 720
aggccctgca acgagtccca gggcaatggc acggtggcct gctcctgcca ggactgtgct 780
gcgtcctgcc ccaccatccc ccagccccag gcactggact ccaccttcta cctgggcggg 840
ctggaaggtg ggctggccct tgtcatcatc ctctgctctg cttttgccct gcttaccacc 900
ttcctggtgg gtacccgcct ggcctcctcc tgtggcaagg acaagacgcc agaccccaag 960
gcaggcatga gcctgtctga caaactcagc ctctccacca acgtcatcct tagccagtgc 1020
ttccagaact ggggcacatg ggtggcctca tggccgctga ccatcctgtt ggtgtccatc 1080
gccgtggtat tggccttgtc aggaggcctg gcctttgtgg aactgaccac ggacccagtg 1140
gagctgtggt cggcccccag cagccaagcc cggagcgaga aggctttcca cgaccagcat 1200
tttggcccct tcctccgaac caaccaggtg atcttgacgg ctcccaaccg gcccagctac 1260
cactacgact ccctgctcct ggggcccaag aacttcagtg gggtcctggc ctctgacctc 1320
ctgctggagc tgctggagct acaggagacg ctgcggcacc tccaggtgtg gtcgcccgag 1380
gagcagcgcc acatctcgct gcaggacatc tgcttcgcgc ccctcaaccc tcacaatgcc 1440
agcctctccg actgctgcat caacagcctc ctgcagtatt tccagagcaa ccgcacgcac 1500
ctgctgctca cggccaacca gacgctgacg ggccagacct cccaggtgga ctggagggac 1560
cactttctct actgtgctaa cgccccactc accttcaagg atggcacagc cctagccctg 1620
agctgcatgg ctgactatgg gggccctgtc ttccccttcc ttgccgtggg tggctacaaa 1680
gggaaggact actctgaggc ggaggccctg attatgacct tctccctcaa caactatgcc 1740
cctggggacc cccggctggc ccaggctaag ctctgggagg cagccttctt ggaggagatg 1800
aaagccttcc agcggcggac agctggcact ttccaggtca cattcatggc tgagcgctcc 1860
ctggaggacg agattaaccg cacgacggcg gaggacctcc ccatcttcgg agtcagctac 1920
atcatcatct tcctgtacat ctccctggcg ctgggcagct actccagctg gcgccgggtg 1980
ccggtggact ccaaggtcac gctgggcctg ggcggggtgg cggtggtgct gggagcagtg 2040
acagcggcca tgggcttctt ctcctacctc ggcgtgccgt cctccctggt gatccttcag 2100
gtggtgcctt tcctggtgtt ggccgtgggc gctgacaaca tcttcatctt tgttctggag 2160
taccagaggc tgccccggag gccgggagag ccgcgggagg cccacatcgg ccgagcgctg 2220
ggcagtgtgg cccctagcat gttgctctgc agcctgtctg aggccatctg cttctttcta 2280
ggggccctga cccctatgcc cgctgtgaag acctttgccc tgatctcggg ctttgccatc 2340
gtcctggact tcttgctgca ggtgtcagcc tttgtggctc tgctttctct ggacagcagg 2400
aggcaggagg cctcccgctt ggacgtctgc tgctgcgtga gcgccccgaa gctgcctgca 2460
cccggccaga gcgagggact cctgcttcga gtcttccgca agttctacgt cccagtcctg 2520
ctgcaccggg tgacacgggc ggtggtgctg ctgctgttca ccggcctctt cggggtgggg 2580
ctctacttca tgtgccacat ccgcgtggga ttggatcagg agctggccct gcccaaggac 2640
tcatacctgc tggactattt cttcttcctg aaccgctact ttgaggtggg ggctcccgtc 2700
tactttgtca ccacgggagg ctacaacttc tccagcgagg cgggcatgaa tgctgtgtgc 2760
tccagtgccg ggtgcgacag ttactcctta acccagaaga tccagtacgc caccgagttc 2820
cccgaggagt cttacctggc catccctgcc tcctcctggg tggatgactt catcgactgg 2880
ctgaccccgt cctcctgctg ccgcctttat gcctttggtg ctaataagga caaattctgc 2940
ccttcgactg tcaactccct agcctgcttg aagaactgcg tgaacttcac actgggccct 3000
gtccggccat ccgtggacca gttccacaag taccttccct ggttcctgag tgacccgccc 3060
aacatcaagt gtcccaaagg tgggctggca gcgtacaaca cctccgtgca tttgggatct 3120
gatggccagg ttttagcctc ccggttcatg gcctaccaca agccgctgcg gaactcggag 3180
gattacactg aggccctgcg ggtgtcacgg gcgctggcgg ccaacatcac ggcccagctg 3240
cggcaggtgc caggcaccga cccggccttc gaggtcttcc cctacacgat caccaacgtg 3300
ttctacgagc agtacctgag cgtggtcccc gagggcctct tcatgctcgc catctgcctg 3360
ctgcccacct tcgtagtctg ctgcctgctg ctgggcatgg acctacgctc cggcctcctc 3420
aacctgttct ccatcgtcat gatcctcgtg gacaccgtgg gcttcatggc cctgtggggc 3480
atcagttaca atgccgtgtc gctcatcaac ctggtcacgg cggtgggcat ctccgtggag 3540
tttgtgtccc acatcacccg ctcctttgca gtcagcaccc ggcccacccg gctggagagg 3600
gccaaggagg ccaccatctc catgggcagc gcggtgtttg ctggcgtggc catgaccaac 3660
ctgccgggca tcctcgtcct gggcctggcc aaggcgcagc tcatccagat cttcttcttc 3720
cgcctcaacc tcctcatcac cgtgctgggt ctgctgcatg gcctggtctt cctgccagtg 3780
gtcctcagct acctcgggcc tgatatcaat gcagctctcg tgctggacca gaagaagaca 3840
gaagaggcca tcggggcccc tgcccacctg gtcccaacat ccacggccag cagcacctat 3900
gtcaactacg gcttccaaca tcccgccaac ggtgtagtgg gcgacagttc tctgccccgc 3960
agtggaccgg acctctga                                               3978

<210> 5
<211> 1325
<212> PRT
<213> Artificial Sequence

<220> 
<223> NPC1L1

<400> 5
Met Ala Asp Thr Gly Leu Arg Gly Trp Leu Leu Trp Ala Leu Leu Leu
 1               5                  10                  15      
His Val Ala Gln Ser Glu Leu Tyr Thr Pro Ile His Gln Pro Gly Tyr
            20                  25                  30          
Cys Ala Phe Tyr Asp Glu Cys Gly Lys Asn Pro Glu Leu Ser Gly Gly
        35                  40                  45              
Leu Ala Pro Leu Ser Asn Val Ser Cys Leu Ser Asn Thr Pro Ala Pro
    50                  55                  60                  
Arg Val Thr Gly Glu His Leu Thr Leu Leu Gln Arg Ile Cys Pro Arg
65                  70                  75                  80  
Leu Tyr Thr Gly Thr Thr Thr Tyr Ala Cys Cys Ser Pro Lys Gln Leu
                85                  90                  95      
Leu Ser Leu Glu Thr Ser Leu Ala Val Thr Lys Ala Leu Leu Thr Arg
            100                 105                 110         
Cys Pro Thr Cys Ser Asp Asn Phe Val Asn Leu His Cys Gln Asn Thr
        115                 120                 125             
Cys Ser Pro Asn Gln Ser Leu Phe Ile Asn Val Thr Arg Val Ala Gly
    130                 135                 140                 
Gly Gly Gly Gly Arg Pro Gln Ala Val Val Ala Tyr Glu Ala Phe Tyr
145                 150                 155                 160 
Gln Asp Thr Phe Ala Gln Gln Thr Tyr Asp Ser Cys Ser Arg Val Arg
                165                 170                 175     
Ile Pro Ala Ala Ala Thr Leu Ala Val Gly Thr Met Cys Gly Val Tyr
            180                 185                 190         
Gly Ser Thr Leu Cys Asn Ala Gln Arg Trp Leu Asn Phe Gln Gly Asp
        195                 200                 205             
Thr Ser Asn Gly Leu Ala Pro Leu Asp Ile Thr Phe His Leu Met Glu
    210                 215                 220                 
Pro Gly Gln Ala Leu Gly Ser Gly Met Gln Ala Leu Thr Gly Glu Ile
225                 230                 235                 240 
Arg Pro Cys Asn Glu Ser Gln Gly Asn Gly Thr Val Ala Cys Ser Cys
                245                 250                 255     
Gln Asp Cys Ala Ala Ser Cys Pro Thr Ile Pro Gln Pro Gln Ala Leu
            260                 265                 270         
Asp Ser Thr Phe Tyr Leu Gly Gly Leu Glu Gly Gly Leu Ala Leu Val
        275                 280                 285             
Ile Ile Leu Cys Ser Ala Phe Ala Leu Leu Thr Thr Phe Leu Val Gly
    290                 295                 300                 
Thr Arg Leu Ala Ser Ser Cys Gly Lys Asp Lys Thr Pro Asp Pro Lys
305                 310                 315                 320 
Ala Gly Met Ser Leu Ser Asp Lys Leu Ser Leu Ser Thr Asn Val Ile
                325                 330                 335     
Leu Ser Gln Cys Phe Gln Asn Trp Gly Thr Trp Val Ala Ser Trp Pro
            340                 345                 350         
Leu Thr Ile Leu Leu Val Ser Ile Ala Val Val Leu Ala Leu Ser Gly
        355                 360                 365             
Gly Leu Ala Phe Val Glu Leu Thr Thr Asp Pro Val Glu Leu Trp Ser
    370                 375                 380                 
Ala Pro Ser Ser Gln Ala Arg Ser Glu Lys Ala Phe His Asp Gln His
385                 390                 395                 400 
Phe Gly Pro Phe Leu Arg Thr Asn Gln Val Ile Leu Thr Ala Pro Asn
                405                 410                 415     
Arg Pro Ser Tyr His Tyr Asp Ser Leu Leu Leu Gly Pro Lys Asn Phe
            420                 425                 430         
Ser Gly Val Leu Ala Ser Asp Leu Leu Leu Glu Leu Leu Glu Leu Gln
        435                 440                 445             
Glu Thr Leu Arg His Leu Gln Val Trp Ser Pro Glu Glu Gln Arg His
    450                 455                 460                 
Ile Ser Leu Gln Asp Ile Cys Phe Ala Pro Leu Asn Pro His Asn Ala
465                 470                 475                 480 
Ser Leu Ser Asp Cys Cys Ile Asn Ser Leu Leu Gln Tyr Phe Gln Ser
                485                 490                 495     
Asn Arg Thr His Leu Leu Leu Thr Ala Asn Gln Thr Leu Thr Gly Gln
            500                 505                 510         
Thr Ser Gln Val Asp Trp Arg Asp His Phe Leu Tyr Cys Ala Asn Ala
        515                 520                 525             
Pro Leu Thr Phe Lys Asp Gly Thr Ala Leu Ala Leu Ser Cys Met Ala
    530                 535                 540                 
Asp Tyr Gly Gly Pro Val Phe Pro Phe Leu Ala Val Gly Gly Tyr Lys
545                 550                 555                 560 
Gly Lys Asp Tyr Ser Glu Ala Glu Ala Leu Ile Met Thr Phe Ser Leu
                565                 570                 575     
Asn Asn Tyr Ala Pro Gly Asp Pro Arg Leu Ala Gln Ala Lys Leu Trp
            580                 585                 590         
Glu Ala Ala Phe Leu Glu Glu Met Lys Ala Phe Gln Arg Arg Thr Ala
        595                 600                 605             
Gly Thr Phe Gln Val Thr Phe Met Ala Glu Arg Ser Leu Glu Asp Glu
    610                 615                 620                 
Ile Asn Arg Thr Thr Ala Glu Asp Leu Pro Ile Phe Gly Val Ser Tyr
625                 630                 635                 640 
Ile Ile Ile Phe Leu Tyr Ile Ser Leu Ala Leu Gly Ser Tyr Ser Ser
                645                 650                 655     
Trp Arg Arg Val Pro Val Asp Ser Lys Val Thr Leu Gly Leu Gly Gly
            660                 665                 670         
Val Ala Val Val Leu Gly Ala Val Thr Ala Ala Met Gly Phe Phe Ser
        675                 680                 685             
Tyr Leu Gly Val Pro Ser Ser Leu Val Ile Leu Gln Val Val Pro Phe
    690                 695                 700                 
Leu Val Leu Ala Val Gly Ala Asp Asn Ile Phe Ile Phe Val Leu Glu
705                 710                 715                 720 
Tyr Gln Arg Leu Pro Arg Arg Pro Gly Glu Pro Arg Glu Ala His Ile
                725                 730                 735     
Gly Arg Ala Leu Gly Ser Val Ala Pro Ser Met Leu Leu Cys Ser Leu
            740                 745                 750         
Ser Glu Ala Ile Cys Phe Phe Leu Gly Ala Leu Thr Pro Met Pro Ala
        755                 760                 765             
Val Lys Thr Phe Ala Leu Ile Ser Gly Phe Ala Ile Val Leu Asp Phe
    770                 775                 780                 
Leu Leu Gln Val Ser Ala Phe Val Ala Leu Leu Ser Leu Asp Ser Arg
785                 790                 795                 800 
Arg Gln Glu Ala Ser Arg Leu Asp Val Cys Cys Cys Val Ser Ala Pro
                805                 810                 815     
Lys Leu Pro Ala Pro Gly Gln Ser Glu Gly Leu Leu Leu Arg Val Phe
            820                 825                 830         
Arg Lys Phe Tyr Val Pro Val Leu Leu His Arg Val Thr Arg Ala Val
        835                 840                 845             
Val Leu Leu Leu Phe Thr Gly Leu Phe Gly Val Gly Leu Tyr Phe Met
    850                 855                 860                 
Cys His Ile Arg Val Gly Leu Asp Gln Glu Leu Ala Leu Pro Lys Asp
865                 870                 875                 880 
Ser Tyr Leu Leu Asp Tyr Phe Phe Phe Leu Asn Arg Tyr Phe Glu Val
                885                 890                 895     
Gly Ala Pro Val Tyr Phe Val Thr Thr Gly Gly Tyr Asn Phe Ser Ser
            900                 905                 910         
Glu Ala Gly Met Asn Ala Val Cys Ser Ser Ala Gly Cys Asp Ser Tyr
        915                 920                 925             
Ser Leu Thr Gln Lys Ile Gln Tyr Ala Thr Glu Phe Pro Glu Glu Ser
    930                 935                 940                 
Tyr Leu Ala Ile Pro Ala Ser Ser Trp Val Asp Asp Phe Ile Asp Trp
945                 950                 955                 960 
Leu Thr Pro Ser Ser Cys Cys Arg Leu Tyr Ala Phe Gly Ala Asn Lys
                965                 970                 975     
Asp Lys Phe Cys Pro Ser Thr Val Asn Ser Leu Ala Cys Leu Lys Asn
            980                 985                 990         
Cys Val Asn Phe Thr Leu Gly Pro Val Arg Pro Ser Val Asp Gln Phe
        995                 1000                1005            
His Lys Tyr Leu Pro Trp Phe Leu Ser Asp Pro Pro Asn Ile Lys Cys
    1010                1015                1020                
Pro Lys Gly Gly Leu Ala Ala Tyr Asn Thr Ser Val His Leu Gly Ser
1025                1030                1035               1040 
Asp Gly Gln Val Leu Ala Ser Arg Phe Met Ala Tyr His Lys Pro Leu
                1045                1050                1055    
Arg Asn Ser Glu Asp Tyr Thr Glu Ala Leu Arg Val Ser Arg Ala Leu
            1060                1065                1070        
Ala Ala Asn Ile Thr Ala Gln Leu Arg Gln Val Pro Gly Thr Asp Pro
        1075                1080                1085            
Ala Phe Glu Val Phe Pro Tyr Thr Ile Thr Asn Val Phe Tyr Glu Gln
    1090                1095                1100                
Tyr Leu Ser Val Val Pro Glu Gly Leu Phe Met Leu Ala Ile Cys Leu
1105                1110                1115               1120 
Leu Pro Thr Phe Val Val Cys Cys Leu Leu Leu Gly Met Asp Leu Arg
                1125                1130                1135    
Ser Gly Leu Leu Asn Leu Phe Ser Ile Val Met Ile Leu Val Asp Thr
            1140                1145                1150        
Val Gly Phe Met Ala Leu Trp Gly Ile Ser Tyr Asn Ala Val Ser Leu
        1155                1160                1165            
Ile Asn Leu Val Thr Ala Val Gly Ile Ser Val Glu Phe Val Ser His
    1170                1175                1180                
Ile Thr Arg Ser Phe Ala Val Ser Thr Arg Pro Thr Arg Leu Glu Arg
1185                1190                1195               1200 
Ala Lys Glu Ala Thr Ile Ser Met Gly Ser Ala Val Phe Ala Gly Val
                1205                1210                1215    
Ala Met Thr Asn Leu Pro Gly Ile Leu Val Leu Gly Leu Ala Lys Ala
            1220                1225                1230        
Gln Leu Ile Gln Ile Phe Phe Phe Arg Leu Asn Leu Leu Ile Thr Val
        1235                1240                1245            
Leu Gly Leu Leu His Gly Leu Val Phe Leu Pro Val Val Leu Ser Tyr
    1250                1255                1260                
Leu Gly Pro Asp Ile Asn Ala Ala Leu Val Leu Asp Gln Lys Lys Thr
1265                1270                1275               1280 
Glu Glu Ala Ile Gly Ala Pro Ala His Leu Val Pro Thr Ser Thr Ala
                1285                1290                1295    
Ser Ser Thr Tyr Val Asn Tyr Gly Phe Gln His Pro Ala Asn Gly Val
            1300                1305                1310        
Val Gly Asp Ser Ser Leu Pro Arg Ser Gly Pro Asp Leu
        1315                1320                1325

