                         SEQUENCE LISTING

<110>  USV Ltd.
       RAO, Laxmi Srinivas  Ph.D.
       AHMED, Monsur Borbhuiya  Ph.D.
       NIPHADKAR, Milind  Ph.D.
       PALIWAL, Dinesh  Ph.D.
       SHEKHAWAT, Rakesh  Ph.D.
       KHARE, Aruna  Ph.D.
       SAMANT, Radhika  Ph.D.
       THAKUR, Priti  Ph.D.
       CHUTKE, Anjali  Ph.D.
       SURLIKAR, Nilesh  Ph.D.
       ZAWAR, Sagar  Ph.D.
       MISHRA, Shrikant  Ph.D.
 
<120>  Synthetic Gene for Enhanced Expression in E. coli

<130>  USV Ltd.

<140>  unassigned
<141>  2008-05-23

<150>  1247/MUM/2007
<151>  2007-06-29

<160>  22    

<170>  PatentIn version 3.5

<210>  1
<211>  96
<212>  DNA
<213>  Synthetic nesiritide gene

<400>  1
agcccgaaaa tggtgcaggg cagcggctgc ttcggccgca aaatggatcg catcagcagc       60

agctcaggcc tgggctgcaa agttctgcgc cgtcat                                 96


<210>  2
<211>  32
<212>  PRT
<213>  Homo sapiens (wild-type nesiritide polypeptide)


<220>
<221>  DISULFID
<222>  (10)..(26)

<400>  2

Ser Pro Lys Met Val Glx Gly Ser Gly Cys Phe Gly Arg Lys Met Asp 
1               5                   10                  15      


Arg Ile Ser Ser Ser Ser Gly Leu Gly Cys Lys Val Leu Arg Arg His 
            20                  25                  30          


<210>  3
<211>  290
<212>  DNA
<213>  synthetic nesiritide with fusion partner

<400>  3
atgggcggtg cgcatcatca tcatcatcac gtcgacaatg ttgatgaaag ctggctacag       60

gaaggccaga cgcgaattat ttttgatggc gttaactcgg cgtttcatct gtggattaat      120

gggcgctggg tcggttacgg ccaggacagt cgtttgccca tgggcggtgc ggtcgacgac      180

gacgacaaga gcccgaaaat ggtgcagggc agcggctgct tcggccgcaa aatggatcgc      240

atcagcagca gctcaggcct gggctgcaaa gttctgcgcc gtattaataa                 290


<210>  4
<211>  28
<212>  DNA
<213>  Synthetic primer

<400>  4
tgcgtctttt actggctctt ctcgctaa                                          28


<210>  5
<211>  36
<212>  DNA
<213>  Synthetic primer

<400>  5
agatctcgag ctcggacatt ttcgggctct tgtcgt                                 36


<210>  6
<211>  33
<212>  DNA
<213>  Synthetic primer

<400>  6
ccagctcgag atgctgccct gcaccatttt cgg                                    33


<210>  7
<211>  30
<212>  DNA
<213>  Synthetic primer

<400>  7
taccagctcg agagaagcag ccgctgccct                                        30


<210>  8
<211>  36
<212>  DNA
<213>  Synthetic primer

<400>  8
catatggctc gagatccatt ttgcggccga agcagc                                 36


<210>  9
<211>  34
<212>  DNA
<213>  Synthetic primer

<400>  9
atatggtacc agctgcgcag cccaggccgc tgct                                   34


<210>  10
<211>  31
<212>  DNA
<213>  Synthetic primer

<400>  10
cactgcgtct tttactggct cttctcgcta a                                      31


<210>  11
<211>  37
<212>  DNA
<213>  Synthetic primer

<400>  11
gcttcgaatt cccatatgcg cagaactttg cagccca                                37


<210>  12
<211>  41
<212>  DNA
<213>  Synthetic primer

<400>  12
gccaaaacag ccaagcttct tattaatgac ggcgcagaac t                           41


<210>  13
<211>  39
<212>  DNA
<213>  Synthetic primer

<400>  13
gaactttgca gaggcctgag ctgctgctga tgcgatcca                              39


<210>  14
<211>  30
<212>  DNA
<213>  Synthetic primer

<400>  14
cgcatcagca gctcaggcct gggctgcaaa                                        30


<210>  15
<211>  24
<212>  DNA
<213>  Synthetic primer

<400>  15
tagctccttc ggtcctccga tcgt                                              24


<210>  16
<211>  51
<212>  DNA
<213>  Synthetic primer

<400>  16
cccgtttttt gggctaacag gaggaattaa ccgtcgacaa tgttgatgaa a                51


<210>  17
<211>  32
<212>  DNA
<213>  Synthetic primer

<400>  17
gatgatggtc gaccgcaccg cccatgggca aa                                     32


<210>  18
<211>  33
<212>  DNA
<213>  Synthetic primer

<400>  18
agaaaccaat tgtccatatt gcatcagaca ttg                                    33


<210>  19
<211>  30
<212>  DNA
<213>  Synthetic primer

<400>  19
cgaccgcacc gacgtcgggc aaacgactgt                                        30


<210>  20
<211>  30
<212>  DNA
<213>  Synthetic primer

<400>  20
gacagtcgtt tgcccgacgt cggtgcggtc                                        30


<210>  21
<211>  46
<212>  DNA
<213>  Synthetic primer

<400>  21
gcggctgcgt tctgatttaa tctcgagcag gctgaaaatc ttctct                      46


<210>  22
<211>  5982
<212>  DNA
<213>  vector pET-RAZ-6-N

<400>  22
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat       60

aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg      120

tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat      180

gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat      240

tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt      300

aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag      360

cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa      420

agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg      480

ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct      540

tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac      600

tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca      660

caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat      720

accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact      780

attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc      840

ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga      900

taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg      960

taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg     1020

aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca     1080

agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta     1140

ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca     1200

ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg     1260

cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga     1320

tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa     1380

tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc     1440

tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg     1500

tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac     1560

ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct     1620

acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc     1680

ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg     1740

gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg     1800

ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct     1860

ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga     1920

taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg     1980

cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca     2040

tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc     2100

gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc     2160

gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt     2220

acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac     2280

cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga     2340

tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc     2400

ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg     2460

tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca     2520

cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac     2580

tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg     2640

ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga     2700

acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga     2760

agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc     2820

gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg     2880

tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga     2940

tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg     3000

tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc     3060

cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg     3120

acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt     3180

ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt     3240

taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg     3300

cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat     3360

aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc     3420

ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt     3480

gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat     3540

cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg     3600

tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg     3660

cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg     3720

tgcctaatga gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc     3780

gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt     3840

gcgtattggg cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc     3900

ccttcaccgc ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca     3960

ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat     4020

cgtcgtatcc cactaccgag atatccgcac caacgcgcag cccggactcg gtaatggcgc     4080

gcattgcgcc cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct     4140

cattcagcat ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt     4200

ccgctatcgg ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac     4260

gcgccgagac agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga     4320

ccagatgctc cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg     4380

gtgtctggtc agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag     4440

caatggcatc ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga     4500

gaagattgtg caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca     4560

ccacgctggc acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg     4620

cgtgcagggc cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt     4680

gttgtgccac gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc     4740

gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga     4800

caccggcata ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt     4860

gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt     4920

ccgggatctc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg     4980

ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac     5040

agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg     5100

aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca     5160

cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc     5220

ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag     5280

aaataatttt gtttaacttt aagaaggaga tataccatgg gcggtgcgca tcatcatcat     5340

catcacgtcg acaatgttga tgaaagctgg ctacaggaag gccagacgcg aattattttt     5400

gatggcgtta actcggcgtt tcatctgtgg attaatgggc gctgggtcgg ttacggccag     5460

gacagtcgtt tgcccgacgt cggtgcggtc gacgacgacg acgacaagag cccgaaaatg     5520

gtgcagggca gcggctgctt cggccgcaaa atggatcgca tcagcagcag ctcaggcctg     5580

ggctgcaaag ttctgcgccg tcattaataa gaagcttggc tgttttggcg gatgagagaa     5640

gattttcagc ctgctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc     5700

tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag     5760

gggttttttg ctgaaaggag gaactatatc cggatatccc gcaagaggcc cggcagtacc     5820

ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc     5880

gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta     5940

ccgcattaaa gcttatcgat gataagctgt caaacatgag aa                        5982


