                         SEQUENCE LISTING

<110>  Danisco US Inc., Genencor Div.
       Bowers, Benjamin S.
       Larenas, Edmund A.
 
<120>  Heterologous and Homologous Cellulase Expression System

<130>  30984WO

<150>  US 60/933,894
<151>  2007-06-08

<160>  14    

<170>  PatentIn version 3.4

<210>  1
<211>  2656
<212>  DNA
<213>  Artificial

<220>
<223>  composite of Trichoderma reesei, Acidothermus cellulolyticus and 
       synthetic sequences

<400>  1
atgtatcgga agttggccgt catctcggcc ttcttggcca cagctcgtgc tcagtcggcc     60

tgcactctcc aatcggagac tcacccgcct ctgacatggc agaaatgctc gtctggtggc    120

acttgcactc aacagacagg ctccgtggtc atcgacgcca actggcgctg gactcacgct    180

acgaacagca gcacgaactg ctacgatggc aacacttgga gctcgaccct atgtcctgac    240

aacgagacct gcgcgaagaa ctgctgtctg gacggtgccg cctacgcgtc cacgtacgga    300

gttaccacga gcggtaacag cctctccatt ggctttgtca cccagtctgc gcagaagaac    360

gttggcgctc gcctttacct tatggcgagc gacacgacct accaggaatt caccctgctt    420

ggcaacgagt tctctttcga tgttgatgtt tcgcagctgc cgtaagtgac ttaccatgaa    480

cccctgacgt atcttcttgt gggctcccag ctgactggcc aatttaaggt gcggcttgaa    540

cggagctctc tacttcgtgt ccatggacgc ggatggtggc gtgagcaagt atcccaccaa    600

caccgctggc gccaagtacg gcacggggta ctgtgacagc cagtgtcccc gcgatctgaa    660

gttcatcaat ggccaggcca acgttgaggg ctgggagccg tcatccaaca acgcaaacac    720

gggcattgga ggacacggaa gctgctgctc tgagatggat atctgggagg ccaactccat    780

ctccgaggct cttacccccc acccttgcac gactgtcggc caggagatct gcgagggtga    840

tgggtgcggc ggaacttact ccgataacag atatggcggc acttgcgatc ccgatggctg    900

cgactggaac ccataccgcc tgggcaacac cagcttctac ggccctggct caagctttac    960

cctcgatacc accaagaaat tgaccgttgt cacccagttc gagacgtcgg gtgccatcaa   1020

ccgatactat gtccagaatg gcgtcacttt ccagcagccc aacgccgagc ttggtagtta   1080

ctctggcaac gagctcaacg atgattactg cacagctgag gaggcagaat tcggcggatc   1140

ctctttctca gacaagggcg gcctgactca gttcaagaag gctacctctg gcggcatggt   1200

tctggtcatg agtctgtggg atgatgtgag tttgatggac aaacatgcgc gttgacaaag   1260

agtcaagcag ctgactgaga tgttacagta ctacgccaac atgctgtggc tggactccac   1320

ctacccgaca aacgagacct cctccacacc cggtgccgtg cgcggaagct gctccaccag   1380

ctccggtgtc cctgctcagg tcgaatctca gtctcccaac gccaaggtca ccttctccaa   1440

catcaagttc ggacccattg gcagcaccgg caaccctagc ggcggcaacc ctcccggcgg   1500

aaacccgcct ggcaccacca ccacccgccg cccagccact accactggaa gctctcccgg   1560

acctactagt aagcgggcgg gcggcggcta ttggcacacg agcggccggg agatcctgga   1620

cgcgaacaac gtgccggtac ggatcgccgg catcaactgg tttgggttcg aaacctgcaa   1680

ttacgtcgtg cacggtctct ggtcacgcga ctaccgcagc atgctcgacc agataaagtc   1740

gctcggctac aacacaatcc ggctgccgta ctctgacgac attctcaagc cgggcaccat   1800

gccgaacagc atcaattttt accagatgaa tcaggacctg cagggtctga cgtccttgca   1860

ggtcatggac aaaatcgtcg cgtacgccgg tcagatcggc ctgcgcatca ttcttgaccg   1920

ccaccgaccg gattgcagcg ggcagtcggc gctgtggtac acgagcagcg tctcggaggc   1980

tacgtggatt tccgacctgc aagcgctggc gcagcgctac aagggaaacc cgacggtcgt   2040

cggctttgac ttgcacaacg agccgcatga cccggcctgc tggggctgcg gcgatccgag   2100

catcgactgg cgattggccg ccgagcgggc cggaaacgcc gtgctctcgg tgaatccgaa   2160

cctgctcatt ttcgtcgaag gtgtgcagag ctacaacgga gactcctact ggtggggcgg   2220

caacctgcaa ggagccggcc agtacccggt cgtgctgaac gtgccgaacc gcctggtgta   2280

ctcggcgcac gactacgcga cgagcgtcta cccgcagacg tggttcagcg atccgacctt   2340

ccccaacaac atgcccggca tctggaacaa gaactgggga tacctcttca atcagaacat   2400

tgcaccggta tggctgggcg aattcggtac gacactgcaa tccacgaccg accagacgtg   2460

gctgaagacg ctcgtccagt acctacggcc gaccgcgcaa tacggtgcgg acagcttcca   2520

gtggaccttc tggtcctgga accccgattc cggcgacaca ggaggaattc tcaaggatga   2580

ctggcagacg gtcgacacag taaaagacgg ctatctcgcg ccgatcaagt cgtcgatttt   2640

cgatcctgtc ggctaa                                                   2656


<210>  2
<211>  841
<212>  PRT
<213>  Artificial

<220>
<223>  composite of T. reesei, Aciothermus cellulyticus and synthetic 
       sequences


<220>
<221>  SIGNAL
<222>  (1)..(17)

<400>  2

Met Tyr Arg Lys Leu Ala Val Ile Ser Ala Phe Leu Ala Thr Ala Arg 
1               5                   10                  15      


Ala Gln Ser Ala Cys Thr Leu Gln Ser Glu Thr His Pro Pro Leu Thr 
            20                  25                  30          


Trp Gln Lys Cys Ser Ser Gly Gly Thr Cys Thr Gln Gln Thr Gly Ser 
        35                  40                  45              


Val Val Ile Asp Ala Asn Trp Arg Trp Thr His Ala Thr Asn Ser Ser 
    50                  55                  60                  


Thr Asn Cys Tyr Asp Gly Asn Thr Trp Ser Ser Thr Leu Cys Pro Asp 
65                  70                  75                  80  


Asn Glu Thr Cys Ala Lys Asn Cys Cys Leu Asp Gly Ala Ala Tyr Ala 
                85                  90                  95      


Ser Thr Tyr Gly Val Thr Thr Ser Gly Asn Ser Leu Ser Ile Gly Phe 
            100                 105                 110         


Val Thr Gln Ser Ala Gln Lys Asn Val Gly Ala Arg Leu Tyr Leu Met 
        115                 120                 125             


Ala Ser Asp Thr Thr Tyr Gln Glu Phe Thr Leu Leu Gly Asn Glu Phe 
    130                 135                 140                 


Ser Phe Asp Val Asp Val Ser Gln Leu Pro Cys Gly Leu Asn Gly Ala 
145                 150                 155                 160 


Leu Tyr Phe Val Ser Met Asp Ala Asp Gly Gly Val Ser Lys Tyr Pro 
                165                 170                 175     


Thr Asn Thr Ala Gly Ala Lys Tyr Gly Thr Gly Tyr Cys Asp Ser Gln 
            180                 185                 190         


Cys Pro Arg Asp Leu Lys Phe Ile Asn Gly Gln Ala Asn Val Glu Gly 
        195                 200                 205             


Trp Glu Pro Ser Ser Asn Asn Ala Asn Thr Gly Ile Gly Gly His Gly 
    210                 215                 220                 


Ser Cys Cys Ser Glu Met Asp Ile Trp Glu Ala Asn Ser Ile Ser Glu 
225                 230                 235                 240 


Ala Leu Thr Pro His Pro Cys Thr Thr Val Gly Gln Glu Ile Cys Glu 
                245                 250                 255     


Gly Asp Gly Cys Gly Gly Thr Tyr Ser Asp Asn Arg Tyr Gly Gly Thr 
            260                 265                 270         


Cys Asp Pro Asp Gly Cys Asp Trp Asn Pro Tyr Arg Leu Gly Asn Thr 
        275                 280                 285             


Ser Phe Tyr Gly Pro Gly Ser Ser Phe Thr Leu Asp Thr Thr Lys Lys 
    290                 295                 300                 


Leu Thr Val Val Thr Gln Phe Glu Thr Ser Gly Ala Ile Asn Arg Tyr 
305                 310                 315                 320 


Tyr Val Gln Asn Gly Val Thr Phe Gln Gln Pro Asn Ala Glu Leu Gly 
                325                 330                 335     


Ser Tyr Ser Gly Asn Glu Leu Asn Asp Asp Tyr Cys Thr Ala Glu Glu 
            340                 345                 350         


Ala Glu Phe Gly Gly Ser Ser Phe Ser Asp Lys Gly Gly Leu Thr Gln 
        355                 360                 365             


Phe Lys Lys Ala Thr Ser Gly Gly Met Val Leu Val Met Ser Leu Trp 
    370                 375                 380                 


Asp Asp Tyr Tyr Ala Asn Met Leu Trp Leu Asp Ser Thr Tyr Pro Thr 
385                 390                 395                 400 


Asn Glu Thr Ser Ser Thr Pro Gly Ala Val Arg Gly Ser Cys Ser Thr 
                405                 410                 415     


Ser Ser Gly Val Pro Ala Gln Val Glu Ser Gln Ser Pro Asn Ala Lys 
            420                 425                 430         


Val Thr Phe Ser Asn Ile Lys Phe Gly Pro Ile Gly Ser Thr Gly Asn 
        435                 440                 445             


Pro Ser Gly Gly Asn Pro Pro Gly Gly Asn Pro Pro Gly Thr Thr Thr 
    450                 455                 460                 


Thr Arg Arg Pro Ala Thr Thr Thr Gly Ser Ser Pro Gly Pro Thr Ser 
465                 470                 475                 480 


Lys Arg Ala Gly Gly Gly Tyr Trp His Thr Ser Gly Arg Glu Ile Leu 
                485                 490                 495     


Asp Ala Asn Asn Val Pro Val Arg Ile Ala Gly Ile Asn Trp Phe Gly 
            500                 505                 510         


Phe Glu Thr Cys Asn Tyr Val Val His Gly Leu Trp Ser Arg Asp Tyr 
        515                 520                 525             


Arg Ser Met Leu Asp Gln Ile Lys Ser Leu Gly Tyr Asn Thr Ile Arg 
    530                 535                 540                 


Leu Pro Tyr Ser Asp Asp Ile Leu Lys Pro Gly Thr Met Pro Asn Ser 
545                 550                 555                 560 


Ile Asn Phe Tyr Gln Met Asn Gln Asp Leu Gln Gly Leu Thr Ser Leu 
                565                 570                 575     


Gln Val Met Asp Lys Ile Val Ala Tyr Ala Gly Gln Ile Gly Leu Arg 
            580                 585                 590         


Ile Ile Leu Asp Arg His Arg Pro Asp Cys Ser Gly Gln Ser Ala Leu 
        595                 600                 605             


Trp Tyr Thr Ser Ser Val Ser Glu Ala Thr Trp Ile Ser Asp Leu Gln 
    610                 615                 620                 


Ala Leu Ala Gln Arg Tyr Lys Gly Asn Pro Thr Val Val Gly Phe Asp 
625                 630                 635                 640 


Leu His Asn Glu Pro His Asp Pro Ala Cys Trp Gly Cys Gly Asp Pro 
                645                 650                 655     


Ser Ile Asp Trp Arg Leu Ala Ala Glu Arg Ala Gly Asn Ala Val Leu 
            660                 665                 670         


Ser Val Asn Pro Asn Leu Leu Ile Phe Val Glu Gly Val Gln Ser Tyr 
        675                 680                 685             


Asn Gly Asp Ser Tyr Trp Trp Gly Gly Asn Leu Gln Gly Ala Gly Gln 
    690                 695                 700                 


Tyr Pro Val Val Leu Asn Val Pro Asn Arg Leu Val Tyr Ser Ala His 
705                 710                 715                 720 


Asp Tyr Ala Thr Ser Val Tyr Pro Gln Thr Trp Phe Ser Asp Pro Thr 
                725                 730                 735     


Phe Pro Asn Asn Met Pro Gly Ile Trp Asn Lys Asn Trp Gly Tyr Leu 
            740                 745                 750         


Phe Asn Gln Asn Ile Ala Pro Val Trp Leu Gly Glu Phe Gly Thr Thr 
        755                 760                 765             


Leu Gln Ser Thr Thr Asp Gln Thr Trp Leu Lys Thr Leu Val Gln Tyr 
    770                 775                 780                 


Leu Arg Pro Thr Ala Gln Tyr Gly Ala Asp Ser Phe Gln Trp Thr Phe 
785                 790                 795                 800 


Trp Ser Trp Asn Pro Asp Ser Gly Asp Thr Gly Gly Ile Leu Lys Asp 
                805                 810                 815     


Asp Trp Gln Thr Val Asp Thr Val Lys Asp Gly Tyr Leu Ala Pro Ile 
            820                 825                 830         


Lys Ser Ser Ile Phe Asp Pro Val Gly 
        835                 840     


<210>  3
<211>  41
<212>  DNA
<213>  Artificial

<220>
<223>  forward PCR primer

<400>  3
gcttatacta gtaagcgcgc gggcggcggc tattggcaca c                         41


<210>  4
<211>  39
<212>  DNA
<213>  Artificial

<220>
<223>  reverse PCR primer

<400>  4
gcttatggcg cgccttagac aggatcgaaa atcgacgac                            39


<210>  5
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  forward PCR primer

<400>  5
caccatgcgt accgccaagt tcgc                                            24


<210>  6
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  reverse PCR primer

<400>  6
ttacaggcac tgagagtacc ag                                              22


<210>  7
<211>  10232
<212>  DNA
<213>  Artificial

<220>
<223>  pTrex3g-Hgrisea-cbh1 expression vector

<400>  7
aagcttacta gtacttctcg agctctgtac atgtccggtc gcgacgtacg cgtatcgatg     60

gcgccagctg caggcggccg cctgcagcca cttgcagtcc cgtggaattc tcacggtgaa    120

tgtaggcctt ttgtagggta ggaattgtca ctcaagcacc cccaacctcc attacgcctc    180

ccccatagag ttcccaatca gtgagtcatg gcactgttct caaatagatt ggggagaagt    240

tgacttccgc ccagagctga aggtcgcaca accgcatgat atagggtcgg caacggcaaa    300

aaagcacgtg gctcaccgaa aagcaagatg tttgcgatct aacatccagg aacctggata    360

catccatcat cacgcacgac cactttgatc tgctggtaaa ctcgtattcg ccctaaaccg    420

aagtgcgtgg taaatctaca cgtgggcccc tttcggtata ctgcgtgtgt cttctctagg    480

tgccattctt ttcccttcct ctagtgttga attgtttgtg ttggagtccg agctgtaact    540

acctctgaat ctctggagaa tggtggacta acgactaccg tgcacctgca tcatgtatat    600

aatagtgatc ctgagaaggg gggtttggag caatgtggga ctttgatggt catcaaacaa    660

agaacgaaga cgcctctttt gcaaagtttt gtttcggcta cggtgaagaa ctggatactt    720

gttgtgtctt ctgtgtattt ttgtggcaac aagaggccag agacaatcta ttcaaacacc    780

aagcttgctc ttttgagcta caagaacctg tggggtatat atctagagtt gtgaagtcgg    840

taatcccgct gtatagtaat acgagtcgca tctaaatact ccgaagctgc tgcgaacccg    900

gagaatcgag atgtgctgga aagcttctag cgagcggcta aattagcatg aaaggctatg    960

agaaattctg gagacggctt gttgaatcat ggcgttccat tcttcgacaa gcaaagcgtt   1020

ccgtcgcagt agcaggcact cattcccgaa aaaactcgga gattcctaag tagcgatgga   1080

accggaataa tataataggc aatacattga gttgcctcga cggttgcaat gcaggggtac   1140

tgagcttgga cataactgtt ccgtacccca cctcttctca acctttggcg tttccctgat   1200

tcagcgtacc cgtacaagtc gtaatcacta ttaacccaga ctgaccggac gtgttttgcc   1260

cttcatttgg agaaataatg tcattgcgat gtgtaatttg cctgcttgac cgactggggc   1320

tgttcgaagc ccgaatgtag gattgttatc cgaactctgc tcgtagaggc atgttgtgaa   1380

tctgtgtcgg gcaggacacg cctcgaaggt tcacggcaag ggaaaccacc gatagcagtg   1440

tctagtagca acctgtaaag ccgcaatgca gcatcactgg aaaatacaaa ccaatggcta   1500

aaagtacata agttaatgcc taaagaagtc atataccagc ggctaataat tgtacaatca   1560

agtggctaaa cgtaccgtaa tttgccaacg gcttgtgggg ttgcagaagc aacggcaaag   1620

ccccacttcc ccacgtttgt ttcttcactc agtccaatct cagctggtga tcccccaatt   1680

gggtcgcttg tttgttccgg tgaagtgaaa gaagacagag gtaagaatgt ctgactcgga   1740

gcgttttgca tacaaccaag ggcagtgatg gaagacagtg aaatgttgac attcaaggag   1800

tatttagcca gggatgcttg agtgtatcgt gtaaggaggt ttgtctgccg atacgacgaa   1860

tactgtatag tcacttctga tgaagtggtc catattgaaa tgtaaagtcg gcactgaaca   1920

ggcaaaagat tgagttgaaa ctgcctaaga tctcgggccc tcgggccttc ggcctttggg   1980

tgtacatgtt tgtgctccgg gcaaatgcaa agtgtggtag gatcgaacac actgctgcct   2040

ttaccaagca gctgagggta tgtgataggc aaatgttcag gggccactgc atggtttcga   2100

atagaaagag aagcttagcc aagaacaata gccgataaag atagcctcat taaacggaat   2160

gagctagtag gcaaagtcag cgaatgtgta tatataaagg ttcgaggtcc gtgcctccct   2220

catgctctcc ccatctactc atcaactcag atcctccagg agacttgtac accatctttt   2280

gaggcacaga aacccaatag tcaaccatca caagtttgta caaaaaagca ggctatgcgt   2340

accgccaagt tcgccaccct cgccgccctt gtggcctcgg ccgccgccca gcaggcgtgc   2400

agtctcacca ccgagaggca cccttccctc tcttggaaga agtgcaccgc cggcggccag   2460

tgccagaccg tccaggcttc catcactctc gactccaact ggcgctggac tcaccaggtg   2520

tctggctcca ccaactgcta cacgggcaac aagtgggata ctagcatctg cactgatgcc   2580

aagtcgtgcg ctcagaactg ctgcgtcgat ggtgccgact acaccagcac ctatggcatc   2640

accaccaacg gtgattccct gagcctcaag ttcgtcacca agggccagca ctcgaccaac   2700

gtcggctcgc gtacctacct gatggacggc gaggacaagt atcagagtac gttctatctt   2760

cagccttctc gcgccttgaa tcctggctaa cgtttacact tcacagcctt cgagctcctc   2820

ggcaacgagt tcaccttcga tgtcgatgtc tccaacatcg gctgcggtct caacggcgcc   2880

ctgtacttcg tctccatgga cgccgatggt ggtctcagcc gctatcctgg caacaaggct   2940

ggtgccaagt acggtaccgg ctactgcgat gctcagtgcc cccgtgacat caagttcatc   3000

aacggcgagg ccaacattga gggctggacc ggctccacca acgaccccaa cgccggcgcg   3060

ggccgctatg gtacctgctg ctctgagatg gatatctggg aagccaacaa catggctact   3120

gccttcactc ctcacccttg caccatcatt ggccagagcc gctgcgaggg cgactcgtgc   3180

ggtggcacct acagcaacga gcgctacgcc ggcgtctgcg accccgatgg ctgcgacttc   3240

aactcgtacc gccagggcaa caagaccttc tacggcaagg gcatgaccgt cgacaccacc   3300

aagaagatca ctgtcgtcac ccagttcctc aaggatgcca acggcgatct cggcgagatc   3360

aagcgcttct acgtccagga tggcaagatc atccccaact ccgagtccac catccccggc   3420

gtcgagggca attccatcac ccaggactgg tgcgaccgcc agaaggttgc ctttggcgac   3480

attgacgact tcaaccgcaa gggcggcatg aagcagatgg gcaaggccct cgccggcccc   3540

atggtcctgg tcatgtccat ctgggatgac cacgcctcca acatgctctg gctcgactcg   3600

accttccctg tcgatgccgc tggcaagccc ggcgccgagc gcggtgcctg cccgaccacc   3660

tcgggtgtcc ctgctgaggt tgaggccgag gcccccaaca gcaacgtcgt cttctccaac   3720

atccgcttcg gccccatcgg ctcgaccgtt gctggtctcc ccggcgcggg caacggcggc   3780

aacaacggcg gcaacccccc gccccccacc accaccacct cctcggctcc ggccaccacc   3840

accaccgcca gcgctggccc caaggctggc cgctggcagc agtgcggcgg catcggcttc   3900

actggcccga cccagtgcga ggagccctac acttgcacca agctcaacga ctggtactct   3960

cagtgcctgt aaacccagct ttcttgtaca aagtggtgat cgcgccagct ccgtgcgaaa   4020

gcctgacgca ccggtagatt cttggtgagc ccgtatcatg acggcggcgg gagctacatg   4080

gccccgggtg atttattttt tttgtatcta cttctgaccc ttttcaaata tacggtcaac   4140

tcatctttca ctggagatgc ggcctgcttg gtattgcgat gttgtcagct tggcaaattg   4200

tggctttcga aaacacaaaa cgattcctta gtagccatgc attttaagat aacggaatag   4260

aagaaagagg aaattaaaaa aaaaaaaaaa acaaacatcc cgttcataac ccgtagaatc   4320

gccgctcttc gtgtatccca gtaccagttt attttgaata gctcgcccgc tggagagcat   4380

cctgaatgca agtaacaacc gtagaggctg acacggcagg tgttgctagg gagcgtcgtg   4440

ttctacaagg ccagacgtct tcgcggttga tatatatgta tgtttgactg caggctgctc   4500

agcgacgaca gtcaagttcg ccctcgctgc ttgtgcaata atcgcagtgg ggaagccaca   4560

ccgtgactcc catctttcag taaagctctg ttggtgttta tcagcaatac acgtaattta   4620

aactcgttag catggggctg atagcttaat taccgtttac cagtgccatg gttctgcagc   4680

tttccttggc ccgtaaaatt cggcgaagcc agccaatcac cagctaggca ccagctaaac   4740

cctataatta gtctcttatc aacaccatcc gctcccccgg gatcaatgag gagaatgagg   4800

gggatgcggg gctaaagaag cctacataac cctcatgcca actcccagtt tacactcgtc   4860

gagccaacat cctgactata agctaacaca gaatgcctca atcctgggaa gaactggccg   4920

ctgataagcg cgcccgcctc gcaaaaacca tccctgatga atggaaagtc cagacgctgc   4980

ctgcggaaga cagcgttatt gatttcccaa agaaatcggg gatcctttca gaggccgaac   5040

tgaagatcac agaggcctcc gctgcagatc ttgtgtccaa gctggcggcc ggagagttga   5100

cctcggtgga agttacgcta gcattctgta aacgggcagc aatcgcccag cagttagtag   5160

ggtcccctct acctctcagg gagatgtaac aacgccacct tatgggacta tcaagctgac   5220

gctggcttct gtgcagacaa actgcgccca cgagttcttc cctgacgccg ctctcgcgca   5280

ggcaagggaa ctcgatgaat actacgcaaa gcacaagaga cccgttggtc cactccatgg   5340

cctccccatc tctctcaaag accagcttcg agtcaaggta caccgttgcc cctaagtcgt   5400

tagatgtccc tttttgtcag ctaacatatg ccaccagggc tacgaaacat caatgggcta   5460

catctcatgg ctaaacaagt acgacgaagg ggactcggtt ctgacaacca tgctccgcaa   5520

agccggtgcc gtcttctacg tcaagacctc tgtcccgcag accctgatgg tctgcgagac   5580

agtcaacaac atcatcgggc gcaccgtcaa cccacgcaac aagaactggt cgtgcggcgg   5640

cagttctggt ggtgagggtg cgatcgttgg gattcgtggt ggcgtcatcg gtgtaggaac   5700

ggatatcggt ggctcgattc gagtgccggc cgcgttcaac ttcctgtacg gtctaaggcc   5760

gagtcatggg cggctgccgt atgcaaagat ggcgaacagc atggagggtc aggagacggt   5820

gcacagcgtt gtcgggccga ttacgcactc tgttgagggt gagtccttcg cctcttcctt   5880

cttttcctgc tctataccag gcctccactg tcctcctttc ttgcttttta tactatatac   5940

gagaccggca gtcactgatg aagtatgtta gacctccgcc tcttcaccaa atccgtcctc   6000

ggtcaggagc catggaaata cgactccaag gtcatcccca tgccctggcg ccagtccgag   6060

tcggacatta ttgcctccaa gatcaagaac ggcgggctca atatcggcta ctacaacttc   6120

gacggcaatg tccttccaca ccctcctatc ctgcgcggcg tggaaaccac cgtcgccgca   6180

ctcgccaaag ccggtcacac cgtgaccccg tggacgccat acaagcacga tttcggccac   6240

gatctcatct cccatatcta cgcggctgac ggcagcgccg acgtaatgcg cgatatcagt   6300

gcatccggcg agccggcgat tccaaatatc aaagacctac tgaacccgaa catcaaagct   6360

gttaacatga acgagctctg ggacacgcat ctccagaagt ggaattacca gatggagtac   6420

cttgagaaat ggcgggaggc tgaagaaaag gccgggaagg aactggacgc catcatcgcg   6480

ccgattacgc ctaccgctgc ggtacggcat gaccagttcc ggtactatgg gtatgcctct   6540

gtgatcaacc tgctggattt cacgagcgtg gttgttccgg ttacctttgc ggataagaac   6600

atcgataaga agaatgagag tttcaaggcg gttagtgagc ttgatgccct cgtgcaggaa   6660

gagtatgatc cggaggcgta ccatggggca ccggttgcag tgcaggttat cggacggaga   6720

ctcagtgaag agaggacgtt ggcgattgca gaggaagtgg ggaagttgct gggaaatgtg   6780

gtgactccat agctaataag tgtcagatag caatttgcac aagaaatcaa taccagcaac   6840

tgtaaataag cgctgaagtg accatgccat gctacgaaag agcagaaaaa aacctgccgt   6900

agaaccgaag agatatgaca cgcttccatc tctcaaagga agaatccctt cagggttgcg   6960

tttccagtct agacacgtat aacggcacaa gtgtctctca ccaaatgggt tatatctcaa   7020

atgtgatcta aggatggaaa gcccagaata tcgatcgcgc gcagatccat atatagggcc   7080

cgggttataa ttacctcagg tcgacgtccc atggccattc gaattcgtaa tcatggtcat   7140

agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa   7200

gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc   7260

gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc   7320

aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact   7380

cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac   7440

ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa   7500

aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg   7560

acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa   7620

gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc   7680

ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac   7740

gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac   7800

cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg   7860

taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt   7920

atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa   7980

cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct   8040

cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga   8100

ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg   8160

ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct   8220

tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt   8280

aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc   8340

tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg   8400

gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag   8460

atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt   8520

tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag   8580

ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt   8640

ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca   8700

tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg   8760

ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat   8820

ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta   8880

tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca   8940

gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct   9000

taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat   9060

cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa   9120

agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt   9180

gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa   9240

ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa   9300

ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg   9360

cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag   9420

cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg   9480

gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc   9540

ataaaattgt aaacgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct   9600

cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagcccg   9660

agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact   9720

ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac   9780

ccaaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga   9840

gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga   9900

aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca   9960

ccacacccgc cgcgcttaat gcgccgctac agggcgcgta ctatggttgc tttgacgtat  10020

gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc  10080

attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca  10140

gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca  10200

gtcacgacgt tgtaaaacga cggccagtgc cc                                10232


<210>  8
<211>  1545
<212>  DNA
<213>  Artificial

<220>
<223>  engineered sequence based on T. reesei

<400>  8
atgtatcgga agttggccgt catctcggcc ttcttggcca cagctcgtgc tcagtcggcc     60

tgcactcttc aaccggagac tcacccgcct ctgacatggc agaaatgctc gtctggtggc    120

acgtgcactc aacagacagg ctccgtggtc atcgacgcca actggcgctg gattcacgct    180

acgaacagca gcacgagctg ctacgatggc aacacttgga gctcgaccct atgtcctgac    240

aacgagacct gcacgaagaa ctgctgtctg gacggtgccg cctacgcgtc cacgtacgga    300

gttaccacga gcggtgacag cctcaccatt ggctttgtca cccagtctgc gcagaagaac    360

gttggcgctc gcctttacct tatggcgaac gacacgacct accaggaatt caccctgctt    420

ggcaacgagt tctctttcga tgttgatgtt tcgcagctgc cgtgcggctt gaacggagct    480

ctctacttcg tgtccatgga cgcggatggt ggcgtgagca agtatcccac caacaccgct    540

ggcgccaagt acggcacggg gtactgtgac agccagtgtc cccgcgatct gaagttcatc    600

aatggccagg ccaacgttga gggctgggag ccgtcaacca acaacgcgaa cacgggcatt    660

ggaggacacg gaagctgctg ctctgagatg gatatctggg aggccaactc tatctccgag    720

gctcttaccc tccacccttg cacgactgtc ggccaggaga tctgcgaggg tgatgggtgc    780

ggcggaactt actccaagaa cagatatggc ggcccttgcg atcccgatgg ctgcgactgg    840

aacccatacc gcctgggcaa caccagcttc tacggccctg gcccaagctt taccctcgat    900

accaccaaga aattgaccgt tgtcacccag ttcaagccgt cgggtgccat caaccgatac    960

tatgtccaga atggcgtcac tttccagcag cccaacgccg agcttggtag ttactctggc   1020

aacgagctca acgatgatta ctgctacgct gaggaggcag aattcggcgg atcctctttc   1080

tcagacaagg gcggcctgac tcagttcaag aaggctacct ctggcggcat ggttctggtc   1140

atgagtctgt gggatgatta ctacgccaac atgctgtggc tggactccac ctacccgaca   1200

aacgagacct cctccacacc cggtgccgtg cgcggaagct gctccaccag ctccggtgac   1260

cctgctcagg tcgaatctca gtttcccaac gccaaggtca ccttctccaa catcaagttc   1320

ggacccattg gcagcaccgg caaccctagc ggcggcaacc ctcccggcgg aaacccgcct   1380

ggcaccacca ccacccgccg cccagccact accactggaa gctctcccgg acctacccag   1440

tctcactacg gccagtgcgg cggtattggc tacagcggcc ccacggtctg cgccagcggc   1500

acaacttgcc aggtcctgaa cccttactac tctcagtgcc tgtaa                   1545


<210>  9
<211>  514
<212>  PRT
<213>  Artificial

<220>
<223>  engineered sequence based on T. reesei

<400>  9

Met Tyr Arg Lys Leu Ala Val Ile Ser Ala Phe Leu Ala Thr Ala Arg 
1               5                   10                  15      


Ala Gln Ser Ala Cys Thr Leu Gln Pro Glu Thr His Pro Pro Leu Thr 
            20                  25                  30          


Trp Gln Lys Cys Ser Ser Gly Gly Thr Cys Thr Gln Gln Thr Gly Ser 
        35                  40                  45              


Val Val Ile Asp Ala Asn Trp Arg Trp Ile His Ala Thr Asn Ser Ser 
    50                  55                  60                  


Thr Ser Cys Tyr Asp Gly Asn Thr Trp Ser Ser Thr Leu Cys Pro Asp 
65                  70                  75                  80  


Asn Glu Thr Cys Thr Lys Asn Cys Cys Leu Asp Gly Ala Ala Tyr Ala 
                85                  90                  95      


Ser Thr Tyr Gly Val Thr Thr Ser Gly Asp Ser Leu Thr Ile Gly Phe 
            100                 105                 110         


Val Thr Gln Ser Ala Gln Lys Asn Val Gly Ala Arg Leu Tyr Leu Met 
        115                 120                 125             


Ala Asn Asp Thr Thr Tyr Gln Glu Phe Thr Leu Leu Gly Asn Glu Phe 
    130                 135                 140                 


Ser Phe Asp Val Asp Val Ser Gln Leu Pro Cys Gly Leu Asn Gly Ala 
145                 150                 155                 160 


Leu Tyr Phe Val Ser Met Asp Ala Asp Gly Gly Val Ser Lys Tyr Pro 
                165                 170                 175     


Thr Asn Thr Ala Gly Ala Lys Tyr Gly Thr Gly Tyr Cys Asp Ser Gln 
            180                 185                 190         


Cys Pro Arg Asp Leu Lys Phe Ile Asn Gly Gln Ala Asn Val Glu Gly 
        195                 200                 205             


Trp Glu Pro Ser Thr Asn Asn Ala Asn Thr Gly Ile Gly Gly His Gly 
    210                 215                 220                 


Ser Cys Cys Ser Glu Met Asp Ile Trp Glu Ala Asn Ser Ile Ser Glu 
225                 230                 235                 240 


Ala Leu Thr Leu His Pro Cys Thr Thr Val Gly Gln Glu Ile Cys Glu 
                245                 250                 255     


Gly Asp Gly Cys Gly Gly Thr Tyr Ser Lys Asn Arg Tyr Gly Gly Pro 
            260                 265                 270         


Cys Asp Pro Asp Gly Cys Asp Trp Asn Pro Tyr Arg Leu Gly Asn Thr 
        275                 280                 285             


Ser Phe Tyr Gly Pro Gly Pro Ser Phe Thr Leu Asp Thr Thr Lys Lys 
    290                 295                 300                 


Leu Thr Val Val Thr Gln Phe Lys Pro Ser Gly Ala Ile Asn Arg Tyr 
305                 310                 315                 320 


Tyr Val Gln Asn Gly Val Thr Phe Gln Gln Pro Asn Ala Glu Leu Gly 
                325                 330                 335     


Ser Tyr Ser Gly Asn Glu Leu Asn Asp Asp Tyr Cys Tyr Ala Glu Glu 
            340                 345                 350         


Ala Glu Phe Gly Gly Ser Ser Phe Ser Asp Lys Gly Gly Leu Thr Gln 
        355                 360                 365             


Phe Lys Lys Ala Thr Ser Gly Gly Met Val Leu Val Met Ser Leu Trp 
    370                 375                 380                 


Asp Asp Tyr Tyr Ala Asn Met Leu Trp Leu Asp Ser Thr Tyr Pro Thr 
385                 390                 395                 400 


Asn Glu Thr Ser Ser Thr Pro Gly Ala Val Arg Gly Ser Cys Ser Thr 
                405                 410                 415     


Ser Ser Gly Asp Pro Ala Gln Val Glu Ser Gln Phe Pro Asn Ala Lys 
            420                 425                 430         


Val Thr Phe Ser Asn Ile Lys Phe Gly Pro Ile Gly Ser Thr Gly Asn 
        435                 440                 445             


Pro Ser Gly Gly Asn Pro Pro Gly Gly Asn Pro Pro Gly Thr Thr Thr 
    450                 455                 460                 


Thr Arg Arg Pro Ala Thr Thr Thr Gly Ser Ser Pro Gly Pro Thr Gln 
465                 470                 475                 480 


Ser His Tyr Gly Gln Cys Gly Gly Ile Gly Tyr Ser Gly Pro Thr Val 
                485                 490                 495     


Cys Ala Ser Gly Thr Thr Cys Gln Val Leu Asn Pro Tyr Tyr Ser Gln 
            500                 505                 510         


Cys Leu 
        


<210>  10
<211>  10145
<212>  DNA
<213>  Artificial

<220>
<223>  pTrex3g-cbh1 expression vector

<400>  10
aagcttacta gtacttctcg agctctgtac atgtccggtc gcgacgtacg cgtatcgatg     60

gcgccagctg caggcggccg cctgcagcca cttgcagtcc cgtggaattc tcacggtgaa    120

tgtaggcctt ttgtagggta ggaattgtca ctcaagcacc cccaacctcc attacgcctc    180

ccccatagag ttcccaatca gtgagtcatg gcactgttct caaatagatt ggggagaagt    240

tgacttccgc ccagagctga aggtcgcaca accgcatgat atagggtcgg caacggcaaa    300

aaagcacgtg gctcaccgaa aagcaagatg tttgcgatct aacatccagg aacctggata    360

catccatcat cacgcacgac cactttgatc tgctggtaaa ctcgtattcg ccctaaaccg    420

aagtgcgtgg taaatctaca cgtgggcccc tttcggtata ctgcgtgtgt cttctctagg    480

tgccattctt ttcccttcct ctagtgttga attgtttgtg ttggagtccg agctgtaact    540

acctctgaat ctctggagaa tggtggacta acgactaccg tgcacctgca tcatgtatat    600

aatagtgatc ctgagaaggg gggtttggag caatgtggga ctttgatggt catcaaacaa    660

agaacgaaga cgcctctttt gcaaagtttt gtttcggcta cggtgaagaa ctggatactt    720

gttgtgtctt ctgtgtattt ttgtggcaac aagaggccag agacaatcta ttcaaacacc    780

aagcttgctc ttttgagcta caagaacctg tggggtatat atctagagtt gtgaagtcgg    840

taatcccgct gtatagtaat acgagtcgca tctaaatact ccgaagctgc tgcgaacccg    900

gagaatcgag atgtgctgga aagcttctag cgagcggcta aattagcatg aaaggctatg    960

agaaattctg gagacggctt gttgaatcat ggcgttccat tcttcgacaa gcaaagcgtt   1020

ccgtcgcagt agcaggcact cattcccgaa aaaactcgga gattcctaag tagcgatgga   1080

accggaataa tataataggc aatacattga gttgcctcga cggttgcaat gcaggggtac   1140

tgagcttgga cataactgtt ccgtacccca cctcttctca acctttggcg tttccctgat   1200

tcagcgtacc cgtacaagtc gtaatcacta ttaacccaga ctgaccggac gtgttttgcc   1260

cttcatttgg agaaataatg tcattgcgat gtgtaatttg cctgcttgac cgactggggc   1320

tgttcgaagc ccgaatgtag gattgttatc cgaactctgc tcgtagaggc atgttgtgaa   1380

tctgtgtcgg gcaggacacg cctcgaaggt tcacggcaag ggaaaccacc gatagcagtg   1440

tctagtagca acctgtaaag ccgcaatgca gcatcactgg aaaatacaaa ccaatggcta   1500

aaagtacata agttaatgcc taaagaagtc atataccagc ggctaataat tgtacaatca   1560

agtggctaaa cgtaccgtaa tttgccaacg gcttgtgggg ttgcagaagc aacggcaaag   1620

ccccacttcc ccacgtttgt ttcttcactc agtccaatct cagctggtga tcccccaatt   1680

gggtcgcttg tttgttccgg tgaagtgaaa gaagacagag gtaagaatgt ctgactcgga   1740

gcgttttgca tacaaccaag ggcagtgatg gaagacagtg aaatgttgac attcaaggag   1800

tatttagcca gggatgcttg agtgtatcgt gtaaggaggt ttgtctgccg atacgacgaa   1860

tactgtatag tcacttctga tgaagtggtc catattgaaa tgtaaagtcg gcactgaaca   1920

ggcaaaagat tgagttgaaa ctgcctaaga tctcgggccc tcgggccttc ggcctttggg   1980

tgtacatgtt tgtgctccgg gcaaatgcaa agtgtggtag gatcgaacac actgctgcct   2040

ttaccaagca gctgagggta tgtgataggc aaatgttcag gggccactgc atggtttcga   2100

atagaaagag aagcttagcc aagaacaata gccgataaag atagcctcat taaacggaat   2160

gagctagtag gcaaagtcag cgaatgtgta tatataaagg ttcgaggtcc gtgcctccct   2220

catgctctcc ccatctactc atcaactcag atcctccagg agacttgtac accatctttt   2280

gaggcacaga aacccaatag tcaaccatca caagtttgta caaaaaacag gctatgtatc   2340

ggaagttggc cgtcatctcg gccttcttgg ccacagctcg tgctcagtcg gcctgcactc   2400

ttcaaccgga gactcacccg cctctgacat ggcagaaatg ctcgtctggt ggcacgtgca   2460

ctcaacagac aggctccgtg gtcatcgacg ccaactggcg ctggattcac gctacgaaca   2520

gcagcacgag ctgctacgat ggcaacactt ggagctcgac cctatgtcct gacaacgaga   2580

cctgcacgaa gaactgctgt ctggacggtg ccgcctacgc gtccacgtac ggagttacca   2640

cgagcggtga cagcctcacc attggctttg tcacccagtc tgcgcagaag aacgttggcg   2700

ctcgccttta ccttatggcg aacgacacga cctaccagga attcaccctg cttggcaacg   2760

agttctcttt cgatgttgat gtttcgcagc tgccgtgcgg cttgaacgga gctctctact   2820

tcgtgtccat ggacgcggat ggtggcgtga gcaagtatcc caccaacacc gctggcgcca   2880

agtacggcac ggggtactgt gacagccagt gtccccgcga tctgaagttc atcaatggcc   2940

aggccaacgt tgagggctgg gagccgtcaa ccaacaacgc gaacacgggc attggaggac   3000

acggaagctg ctgctctgag atggatatct gggaggccaa ctctatctcc gaggctctta   3060

ccctccaccc ttgcacgact gtcggccagg agatctgcga gggtgatggg tgcggcggaa   3120

cttactccaa gaacagatat ggcggccctt gcgatcccga tggctgcgac tggaacccat   3180

accgcctggg caacaccagc ttctacggcc ctggcccaag ctttaccctc gataccacca   3240

agaaattgac cgttgtcacc cagttcaagc cgtcgggtgc catcaaccga tactatgtcc   3300

agaatggcgt cactttccag cagcccaacg ccgagcttgg tagttactct ggcaacgagc   3360

tcaacgatga ttactgctac gctgaggagg cagaattcgg cggatcctct ttctcagaca   3420

agggcggcct gactcagttc aagaaggcta cctctggcgg catggttctg gtcatgagtc   3480

tgtgggatga ttactacgcc aacatgctgt ggctggactc cacctacccg acaaacgaga   3540

cctcctccac acccggtgcc gtgcgcggaa gctgctccac cagctccggt gaccctgctc   3600

aggtcgaatc tcagtttccc aacgccaagg tcaccttctc caacatcaag ttcggaccca   3660

ttggcagcac cggcaaccct agcggcggca accctcccgg cggaaacccg cctggcacca   3720

ccaccacccg ccgcccagcc actaccactg gaagctctcc cggacctacc cagtctcact   3780

acggccagtg cggcggtatt ggctacagcg gccccacggt ctgcgccagc ggcacaactt   3840

gccaggtcct gaacccttac tactctcagt gcctgtaaac ccagctttct tgtacaaagt   3900

ggtgatcgcg ccgcgcgcca gctccgtgcg aaagcctgac gcaccggtag attcttggtg   3960

agcccgtatc atgacggcgg cgggagctac atggccccgg gtgatttatt ttttttgtat   4020

ctacttctga cccttttcaa atatacggtc aactcatctt tcactggaga tgcggcctgc   4080

ttggtattgc gatgttgtca gcttggcaaa ttgtggcttt cgaaaacaca aaacgattcc   4140

ttagtagcca tgcattttaa gataacggaa tagaagaaag aggaaattaa aaaaaaaaaa   4200

aaaacaaaca tcccgttcat aacccgtaga atcgccgctc ttcgtgtatc ccagtaccag   4260

tttattttga atagctcgcc cgctggagag catcctgaat gcaagtaaca accgtagagg   4320

ctgacacggc aggtgttgct agggagcgtc gtgttctaca aggccagacg tcttcgcggt   4380

tgatatatat gtatgtttga ctgcaggctg ctcagcgacg acagtcaagt tcgccctcgc   4440

tgcttgtgca ataatcgcag tggggaagcc acaccgtgac tcccatcttt cagtaaagct   4500

ctgttggtgt ttatcagcaa tacacgtaat ttaaactcgt tagcatgggg ctgatagctt   4560

aattaccgtt taccagtgcc atggttctgc agctttcctt ggcccgtaaa attcggcgaa   4620

gccagccaat caccagctag gcaccagcta aaccctataa ttagtctctt atcaacacca   4680

tccgctcccc cgggatcaat gaggagaatg agggggatgc ggggctaaag aagcctacat   4740

aaccctcatg ccaactccca gtttacactc gtcgagccaa catcctgact ataagctaac   4800

acagaatgcc tcaatcctgg gaagaactgg ccgctgataa gcgcgcccgc ctcgcaaaaa   4860

ccatccctga tgaatggaaa gtccagacgc tgcctgcgga agacagcgtt attgatttcc   4920

caaagaaatc ggggatcctt tcagaggccg aactgaagat cacagaggcc tccgctgcag   4980

atcttgtgtc caagctggcg gccggagagt tgacctcggt ggaagttacg ctagcattct   5040

gtaaacgggc agcaatcgcc cagcagttag tagggtcccc tctacctctc agggagatgt   5100

aacaacgcca ccttatggga ctatcaagct gacgctggct tctgtgcaga caaactgcgc   5160

ccacgagttc ttccctgacg ccgctctcgc gcaggcaagg gaactcgatg aatactacgc   5220

aaagcacaag agacccgttg gtccactcca tggcctcccc atctctctca aagaccagct   5280

tcgagtcaag gtacaccgtt gcccctaagt cgttagatgt ccctttttgt cagctaacat   5340

atgccaccag ggctacgaaa catcaatggg ctacatctca tggctaaaca agtacgacga   5400

aggggactcg gttctgacaa ccatgctccg caaagccggt gccgtcttct acgtcaagac   5460

ctctgtcccg cagaccctga tggtctgcga gacagtcaac aacatcatcg ggcgcaccgt   5520

caacccacgc aacaagaact ggtcgtgcgg cggcagttct ggtggtgagg gtgcgatcgt   5580

tgggattcgt ggtggcgtca tcggtgtagg aacggatatc ggtggctcga ttcgagtgcc   5640

ggccgcgttc aacttcctgt acggtctaag gccgagtcat gggcggctgc cgtatgcaaa   5700

gatggcgaac agcatggagg gtcaggagac ggtgcacagc gttgtcgggc cgattacgca   5760

ctctgttgag ggtgagtcct tcgcctcttc cttcttttcc tgctctatac caggcctcca   5820

ctgtcctcct ttcttgcttt ttatactata tacgagaccg gcagtcactg atgaagtatg   5880

ttagacctcc gcctcttcac caaatccgtc ctcggtcagg agccatggaa atacgactcc   5940

aaggtcatcc ccatgccctg gcgccagtcc gagtcggaca ttattgcctc caagatcaag   6000

aacggcgggc tcaatatcgg ctactacaac ttcgacggca atgtccttcc acaccctcct   6060

atcctgcgcg gcgtggaaac caccgtcgcc gcactcgcca aagccggtca caccgtgacc   6120

ccgtggacgc catacaagca cgatttcggc cacgatctca tctcccatat ctacgcggct   6180

gacggcagcg ccgacgtaat gcgcgatatc agtgcatccg gcgagccggc gattccaaat   6240

atcaaagacc tactgaaccc gaacatcaaa gctgttaaca tgaacgagct ctgggacacg   6300

catctccaga agtggaatta ccagatggag taccttgaga aatggcggga ggctgaagaa   6360

aaggccggga aggaactgga cgccatcatc gcgccgatta cgcctaccgc tgcggtacgg   6420

catgaccagt tccggtacta tgggtatgcc tctgtgatca acctgctgga tttcacgagc   6480

gtggttgttc cggttacctt tgcggataag aacatcgata agaagaatga gagtttcaag   6540

gcggttagtg agcttgatgc cctcgtgcag gaagagtatg atccggaggc gtaccatggg   6600

gcaccggttg cagtgcaggt tatcggacgg agactcagtg aagagaggac gttggcgatt   6660

gcagaggaag tggggaagtt gctgggaaat gtggtgactc catagctaat aagtgtcaga   6720

tagcaatttg cacaagaaat caataccagc aactgtaaat aagcgctgaa gtgaccatgc   6780

catgctacga aagagcagaa aaaaacctgc cgtagaaccg aagagatatg acacgcttcc   6840

atctctcaaa ggaagaatcc cttcagggtt gcgtttccag tctagacacg tataacggca   6900

caagtgtctc tcaccaaatg ggttatatct caaatgtgat ctaaggatgg aaagcccaga   6960

atatcgatcg cgcgcagatc catatatagg gcccgggtta taattacctc aggtcgacgt   7020

cccatggcca ttcgaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc   7080

cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct   7140

aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa   7200

acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta   7260

ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc   7320

gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg   7380

caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt   7440

tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa   7500

gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct   7560

ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc   7620

cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg   7680

tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct   7740

tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag   7800

cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga   7860

agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga   7920

agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg   7980

gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag   8040

aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag   8100

ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat   8160

gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct   8220

taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac   8280

tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa   8340

tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg   8400

gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt   8460

gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca   8520

ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt   8580

cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct   8640

tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg   8700

cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg   8760

agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg   8820

cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa   8880

aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt   8940

aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt   9000

gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt   9060

gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca   9120

tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat   9180

ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata   9240

aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc   9300

tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca   9360

gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg   9420

cggcatcaga gcagattgta ctgagagtgc accataaaat tgtaaacgtt aatattttgt   9480

taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg   9540

gcaaaatccc ttataaatca aaagaatagc ccgagatagg gttgagtgtt gttccagttt   9600

ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct   9660

atcagggcga tggcccacta cgtgaaccat cacccaaatc aagttttttg gggtcgaggt   9720

gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa   9780

agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc   9840

tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc   9900

tacagggcgc gtactatggt tgctttgacg tatgcggtgt gaaataccgc acagatgcgt   9960

aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg  10020

gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag  10080

gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag  10140

tgccc                                                              10145


<210>  11
<211>  1416
<212>  DNA
<213>  Artificial

<220>
<223>  engineered sequence based on T. reesei

<400>  11
atgattgtcg gcattctcac cacgctggct acgctggcca cactcgcagc tagtgtgcct     60

ctagaggagc ggcaagcttg ctcaagcgtc tggggccaat gtggtggcca gaattggtcg    120

ggtccgactt gctgtgcttc cggaagcaca tgcgtctact ccaacgacta ttactcccag    180

tgtcttcccg gcgctgcaag ctcaagctcg tccacgcgcg ccgcgtcgac gacttctcga    240

gtatccccca caacatcccg gtcgagctcc gcgacgcctc cacctggttc tactactacc    300

agagtacctc cagtcggatc gggaaccgct acgtattcag gcaacccttt tgttggggtc    360

actctttggg ccaatgcata ttacgcctct gaagttagca gcctcgctat tcctagcttg    420

actggagcca tggccactgc tgcagcagct gtcgcaaagg ttccctcttt tgtgtggcta    480

gatactcttg acaagacccc tctcatggag caaaccttgg ccgacatccg cgccgccaac    540

aagaatggcg gtaactatgc cggacagttt gtggtgtatg acttgccgga tcgcgattgc    600

gctgcccttg cctcgaatgg cgaatactct attgccgatg gtggcgtcgc caaatataag    660

aactatatcg acaccattcg tcaaattgtc gtggaatatt ccgatgtccg gaccctcctg    720

gttattgagc ctgactctct tgccaacctg gtgaccaacc tcggtactcc aaagtgtgcc    780

aatgctcagt cagcctacct tgagtgcatc aactacgccg tcacacagct gaaccttcca    840

aatgttgcga tgtatttgga cgctggccat gcaggatggc ttggctggcc ggcaaaccaa    900

gacccggccg ctcagctatt tgcaaatgtt tacaagaatg catcgtctcc gagagctctt    960

cgcggattgg caaccaatgt cgccaactac aacgggtgga acattaccag ccccccaccg   1020

tacacgcaag gcaacgctgt ctacaacgag aagctgtaca tccacgctat tggacctctt   1080

cttgccaatc acggctggtc caacgccttc ttcatcactg atcaaggtcg atcgggaaag   1140

cagcctaccg gacagcaaca gtggggagac tggtgcaatg tgatcggcac cggatttggt   1200

attcgcccat ccgcaaacac tggggactcg ttgctggatt cgtttgtctg ggtcaagcca   1260

ggcggcgagt gtgacggcac cagcgacagc agtgcgccac gatttgacta ccactgtgcg   1320

ctcccagatg ccttgcaacc ggcgcctcaa gctggtgctt ggttccaagc ctactttgtg   1380

cagcttctca caaacgcaaa cccatcgttc ctgtaa                             1416


<210>  12
<211>  471
<212>  PRT
<213>  Artificial

<220>
<223>  engineered sequence based on T. reesei

<400>  12

Met Ile Val Gly Ile Leu Thr Thr Leu Ala Thr Leu Ala Thr Leu Ala 
1               5                   10                  15      


Ala Ser Val Pro Leu Glu Glu Arg Gln Ala Cys Ser Ser Val Trp Gly 
            20                  25                  30          


Gln Cys Gly Gly Gln Asn Trp Ser Gly Pro Thr Cys Cys Ala Ser Gly 
        35                  40                  45              


Ser Thr Cys Val Tyr Ser Asn Asp Tyr Tyr Ser Gln Cys Leu Pro Gly 
    50                  55                  60                  


Ala Ala Ser Ser Ser Ser Ser Thr Arg Ala Ala Ser Thr Thr Ser Arg 
65                  70                  75                  80  


Val Ser Pro Thr Thr Ser Arg Ser Ser Ser Ala Thr Pro Pro Pro Gly 
                85                  90                  95      


Ser Thr Thr Thr Arg Val Pro Pro Val Gly Ser Gly Thr Ala Thr Tyr 
            100                 105                 110         


Ser Gly Asn Pro Phe Val Gly Val Thr Pro Trp Ala Asn Ala Tyr Tyr 
        115                 120                 125             


Ala Ser Glu Val Ser Ser Leu Ala Ile Pro Ser Leu Thr Gly Ala Met 
    130                 135                 140                 


Ala Thr Ala Ala Ala Ala Val Ala Lys Val Pro Ser Phe Met Trp Leu 
145                 150                 155                 160 


Asp Thr Leu Asp Lys Thr Pro Leu Met Glu Gln Thr Leu Ala Asp Ile 
                165                 170                 175     


Arg Thr Ala Asn Lys Asn Gly Gly Asn Tyr Ala Gly Gln Phe Val Val 
            180                 185                 190         


Tyr Asp Leu Pro Asp Arg Asp Cys Ala Ala Leu Ala Ser Asn Gly Glu 
        195                 200                 205             


Tyr Ser Ile Ala Asp Gly Gly Val Ala Lys Tyr Lys Asn Tyr Ile Asp 
    210                 215                 220                 


Thr Ile Arg Gln Ile Val Val Glu Tyr Ser Asp Ile Arg Thr Leu Leu 
225                 230                 235                 240 


Val Ile Glu Pro Asp Ser Leu Ala Asn Leu Val Thr Asn Leu Gly Thr 
                245                 250                 255     


Pro Lys Cys Ala Asn Ala Gln Ser Ala Tyr Leu Glu Cys Ile Asn Tyr 
            260                 265                 270         


Ala Val Thr Gln Leu Asn Leu Pro Asn Val Ala Met Tyr Leu Asp Ala 
        275                 280                 285             


Gly His Ala Gly Trp Leu Gly Trp Pro Ala Asn Gln Asp Pro Ala Ala 
    290                 295                 300                 


Gln Leu Phe Ala Asn Val Tyr Lys Asn Ala Ser Ser Pro Arg Ala Leu 
305                 310                 315                 320 


Arg Gly Leu Ala Thr Asn Val Ala Asn Tyr Asn Gly Trp Asn Ile Thr 
                325                 330                 335     


Ser Pro Pro Ser Tyr Thr Gln Gly Asn Ala Val Tyr Asn Glu Lys Leu 
            340                 345                 350         


Tyr Ile His Ala Ile Gly Pro Leu Leu Ala Asn His Gly Trp Ser Asn 
        355                 360                 365             


Ala Phe Phe Ile Thr Asp Gln Gly Arg Ser Gly Lys Gln Pro Thr Gly 
    370                 375                 380                 


Gln Gln Gln Trp Gly Asp Trp Cys Asn Val Ile Gly Thr Gly Phe Gly 
385                 390                 395                 400 


Ile Arg Pro Ser Ala Asn Thr Gly Asp Ser Leu Leu Asp Ser Phe Val 
                405                 410                 415     


Trp Val Lys Pro Gly Gly Glu Cys Asp Gly Thr Ser Asp Ser Ser Ala 
            420                 425                 430         


Pro Arg Phe Asp Ser His Cys Ala Leu Pro Asp Ala Leu Gln Pro Ala 
        435                 440                 445             


Pro Gln Ala Gly Ala Trp Phe Gln Ala Tyr Phe Val Gln Leu Leu Thr 
    450                 455                 460                 


Asn Ala Asn Pro Ser Phe Leu 
465                 470     


<210>  13
<211>  14158
<212>  DNA
<213>  Artificial

<220>
<223>  expression vector pExp-cbhII

<400>  13
gcggccgccg gggtagacga agtgacacgt atccgaaaca gcagtggtat tatggcagct     60

cagcggcatc aaacacgaac ctgagctggc catcgctgag ctgacaagag ccccgccgag    120

cagccatcgc tgaagcgcca tccttatgag caaggaaggg agttattttc gaggatggaa    180

atcttgagtg gatgtctgat ctaggttctt tattgcccag agctgtccct tttaatactc    240

tcgacatcta aaagtttttt ctcctcagcg gtctagcccg cattagcagc agtttcgtca    300

aagcttacgg ctgcatttgc acaccgaggt cgatgtgcca agagctgggg tgctgagagc    360

tggacaatga ttctccactt cagtgttgtt atcggtttcg agcttccact tgaagttagc    420

aggtgcgagt cgctatctct gtagttgagg acgggaccat ttgtactttg ttgtatgtag    480

cctctgcagt ggttggtcct gaataatctt tgaatactcc ggccggctgt gcatttccgt    540

tctctacagc gcagcatctg actagttgta tcgaaccatt agtccgtata gtatcgcatg    600

caattgctag tcaatggtag cagatcagtc gaaggcgtga agtcaatata cgattgcatt    660

gcccgccttg ttatgacaaa cgtaccgagg aagagaagac agtgtatgcc tctatgtatc    720

aaataaggag ccaggaacct cattacccgt atgctattat cgagtggcac tacatgatct    780

ccgaaaaatt taaaaaagaa ataaaaaatt gtcgttaggt ttttacagca agctctcttg    840

ggttatcgga ggctggctgg ttggagcttg tgcagtctct ttgctgatcg agaagattag    900

catgtttctt tcacaatgca aaagaagtat tgctaggaag gttcgaaaga acacttactc    960

ttctacacag tcatttcctg gagactaaca gagctttatg tagtatatat ggagacgtga   1020

agctactgcc gggtgcatgg cttgcccatc accgcacgag ttcgagcacg ttaatattcc   1080

aattacgact caaatcaata cctttgtcaa tgggagctcg tcttgacatt aacgcatcct   1140

ttcaagtaat gcaatgcagc aatggaggaa cttgtagaga ccgagggagg aatggcgaag   1200

ggcggccgga gcttggagtc ctggtggagg ctgaaagctt cgagtttcag cgtctcccag   1260

aagttaccca acccaagtgg ctacaacgac aataagtatt ctatacctag taatattgtt   1320

cgatgcttgt atggagtaga tgctggagtc tggtgtaata ttaatggctt agttcatact   1380

acatttgaca tttccagccc gagagcgcac cgaagccaca tgccgcatat tgacaaagtg   1440

ctagattgtg taaggagggc attctctata gaggaatcag cgtttgcata tacctactac   1500

gtcattgccc taatggacag taagctagcc agctgcatta tgataagagt aacgtgagat   1560

aggtaataag tcttacaaca ctttccctta tagccactaa actacaacat cgtcctgcag   1620

ttcctatatg atacgtataa cccattgata catccaagta tccagaggtg tatggaaata   1680

tcagatcaag acctctctct tctaagaaac ctagaaccag acgctggtag tataataagc   1740

acactgtgac tcgcttaggc ccttaagctt aggccggctt gcttactatt aacctctcat   1800

aaacgctact gcaatgattg gaaacttctt atagtagaat gaggcaataa gacgcatctc   1860

aggtcacata tagtcttatg tttgaaaccc ctcactactg ccatttatct tgtggaaata   1920

tctattattt cagtctatac gtaatgaagg cacttttcag gatctcttcc ctaagcttgt   1980

ataagcaggt ttgttgccgt aaccattctg tctcctcgcc taatacctgt gaagcacaga   2040

atacgtttat tctataagag acgtcttacc ttccatcgag attgaaagct taaaccgtct   2100

acaacggatg ccctcatcat gacccgtcta actcgaacat ctgccacatt agtctcgggt   2160

aacaggagga gtaacacgac cagtgtaaca cgttaagcat acaattgaac gagaatggtg   2220

aggactgaga taaaagaatt ctgttaagga tctaaaatta tagtgcatac aaggtagatg   2280

ttagtaggtg gtttcagttt tcctttcctt tacgttggta tagagcagcg ttcaccaaat   2340

gttagcagag ttctatctat gtcgtatcca ttctgcctta tatctctcaa gggcgccgag   2400

ctcatcctac gaagctctca ggccatcgta ggaaatacag gatagacact gaattctagg   2460

ctaggtatgc gaggcacgcg gatctagggc agactgggca ttgcatagct atggtgtagt   2520

agaactcccg tcaacggcta ttctcaccta gactttcccc ttcgaactga caagttgtta   2580

tattgcctgt gtaccaagcg ctaatgtgga caggattaat gccagagttc attagcctca   2640

agtagagcct atttcctcgc cggaaagtca tctctcttat tgcatttctg cccttcccac   2700

taactcaggg tgcagcgcaa cactacacgc aacatataca ctttattagc cgtgcaacaa   2760

ggctattcta cgaaaaatgc tacactccac atgttaaagg cgcattcaac cagcttcttt   2820

attgggtaat atacagccag gcggggatga agctcattag ccgccactca aggctataca   2880

atgttgccaa ctctccgggc tttatcctgt gctcccgaat accacatcgt gatgatgctt   2940

cagcgcacgg aagtcacaga caccgcctgt ataaaagggg gactgtgacc ctgtatgagg   3000

cgcaacatgg tctcacagca gctcacctga agaggcttgt aagatcaccc taggctgtgt   3060

attgcaccat gattgtcggc attctcacca cgctggctac gctggccaca ctcgcagcta   3120

gtgtgcctct agaggagcgg caagcttgct caagcgtctg gggccaatgt ggtggccaga   3180

attggtcggg tccgacttgc tgtgcttccg gaagcacatg cgtctactcc aacgactatt   3240

actcccagtg tcttcccggc gctgcaagct caagctcgtc cacgcgcgcc gcgtcgacga   3300

cttctcgagt atcccccaca acatcccggt cgagctccgc gacgcctcca cctggttcta   3360

ctactaccag agtacctcca gtcggatcgg gaaccgctac gtattcaggc aacccttttg   3420

ttggggtcac tctttgggcc aatgcatatt acgcctctga agttagcagc ctcgctattc   3480

ctagcttgac tggagccatg gccactgctg cagcagctgt cgcaaaggtt ccctcttttg   3540

tgtggctaga tactcttgac aagacccctc tcatggagca aaccttggcc gacatccgcg   3600

ccgccaacaa gaatggcggt aactatgccg gacagtttgt ggtgtatgac ttgccggatc   3660

gcgattgcgc tgcccttgcc tcgaatggcg aatactctat tgccgatggt ggcgtcgcca   3720

aatataagaa ctatatcgac accattcgtc aaattgtcgt ggaatattcc gatgtccgga   3780

ccctcctggt tattgagcct gactctcttg ccaacctggt gaccaacctc ggtactccaa   3840

agtgtgccaa tgctcagtca gcctaccttg agtgcatcaa ctacgccgtc acacagctga   3900

accttccaaa tgttgcgatg tatttggacg ctggccatgc aggatggctt ggctggccgg   3960

caaaccaaga cccggccgct cagctatttg caaatgttta caagaatgca tcgtctccga   4020

gagctcttcg cggattggca accaatgtcg ccaactacaa cgggtggaac attaccagcc   4080

ccccaccgta cacgcaaggc aacgctgtct acaacgagaa gctgtacatc cacgctattg   4140

gacctcttct tgccaatcac ggctggtcca acgccttctt catcactgat caaggtcgat   4200

cgggaaagca gcctaccgga cagcaacagt ggggagactg gtgcaatgtg atcggcaccg   4260

gatttggtat tcgcccatcc gcaaacactg gggactcgtt gctggattcg tttgtctggg   4320

tcaagccagg cggcgagtgt gacggcacca gcgacagcag tgcgccacga tttgactacc   4380

actgtgcgct cccagatgcc ttgcaaccgg cgcctcaagc tggtgcttgg ttccaagcct   4440

actttgtgca gcttctcaca aacgcaaacc catcgttcct gtaaggcgcg cctaaggctt   4500

tcgtgaccgg gcttcaaaca atgatgtgcg atggtgtggt tcccggttgg cggagtcttt   4560

gtctactttg gttgtctgtc gcaggtcggt agaccgcaaa tgagcaactg atggattgtt   4620

gccagcgata ctataattca catggatggt ctttgtcgat cagtagctag tgagagagag   4680

agaacatcta tccacaatgt cgagtgtcta ttagacatac tccgagaata aagtcaactg   4740

tgtctgtgat ctaaagatcg attcggcagt cgagtagcgt ataacaactc cgagtaccag   4800

caaaagcacg tcgtgacagg agcagggctt tgccaactgc gcaaccttaa ttaaaatagc   4860

tcgcccgctg gagagcatcc tgaatgcaag taacaaccgt agaggctgac acggcaggtg   4920

ttgctaggga gcgtcgtgtt ctacaaggcc agacgtcttc gcggttgata tatatgtatg   4980

tttgactgca ggctgctcag cgacgacagt caagttcgcc ctcgctgctt gtgcaataat   5040

cgcagtgggg aagccacacc gtgactccca tctttcagta aagctctgtt ggtgtttatc   5100

agcaatacac gtaatttaaa ctcgttagca tggggctgat agcttaatta ccgtttacca   5160

gtgccatggt tctgcagctt tccttggccc gtaaaattcg gcgaagccag ccaatcacca   5220

gctaggcacc agctaaaccc tataattagt ctcttatcaa caccatccgc tcccccggga   5280

tcaatgagga gaatgagggg gatgcggggc taaagaagcc tacataaccc tcatgccaac   5340

tcccagttta cactcgtcga gccaacatcc tgactataag ctaacacaga atgcctcaat   5400

cctgggaaga actggccgct gataagcgcg cccgcctcgc aaaaaccatc cctgatgaat   5460

ggaaagtcca gacgctgcct gcggaagaca gcgttattga tttcccaaag aaatcgggga   5520

tcctttcaga ggccgaactg aagatcacag aggcctccgc tgcagatctt gtgtccaagc   5580

tggcggccgg agagttgacc tcggtggaag ttacgctagc attctgtaaa cgggcagcaa   5640

tcgcccagca gttagtaggg tcccctctac ctctcaggga gatgtaacaa cgccacctta   5700

tgggactatc aagctgacgc tggcttctgt gcagacaaac tgcgcccacg agttcttccc   5760

tgacgccgct ctcgcgcagg caagggaact cgatgaatac tacgcaaagc acaagagacc   5820

cgttggtcca ctccatggcc tccccatctc tctcaaagac cagcttcgag tcaaggtaca   5880

ccgttgcccc taagtcgtta gatgtccctt tttgtcagct aacatatgcc accagggcta   5940

cgaaacatca atgggctaca tctcatggct aaacaagtac gacgaagggg actcggttct   6000

gacaaccatg ctccgcaaag ccggtgccgt cttctacgtc aagacctctg tcccgcagac   6060

cctgatggtc tgcgagacag tcaacaacat catcgggcgc accgtcaacc cacgcaacaa   6120

gaactggtcg tgcggcggca gttctggtgg tgagggtgcg atcgttggga ttcgtggtgg   6180

cgtcatcggt gtaggaacgg atatcggtgg ctcgattcga gtgccggccg cgttcaactt   6240

cctgtacggt ctaaggccga gtcatgggcg gctgccgtat gcaaagatgg cgaacagcat   6300

ggagggtcag gagacggtgc acagcgttgt cgggccgatt acgcactctg ttgagggtga   6360

gtccttcgcc tcttccttct tttcctgctc tataccaggc ctccactgtc ctcctttctt   6420

gctttttata ctatatacga gaccggcagt cactgatgaa gtatgttaga cctccgcctc   6480

ttcaccaaat ccgtcctcgg tcaggagcca tggaaatacg actccaaggt catccccatg   6540

ccctggcgcc agtccgagtc ggacattatt gcctccaaga tcaagaacgg cgggctcaat   6600

atcggctact acaacttcga cggcaatgtc cttccacacc ctcctatcct gcgcggcgtg   6660

gaaaccaccg tcgccgcact cgccaaagcc ggtcacaccg tgaccccgtg gacgccatac   6720

aagcacgatt tcggccacga tctcatctcc catatctacg cggctgacgg cagcgccgac   6780

gtaatgcgcg atatcagtgc atccggcgag ccggcgattc caaatatcaa agacctactg   6840

aacccgaaca tcaaagctgt taacatgaac gagctctggg acacgcatct ccagaagtgg   6900

aattaccaga tggagtacct tgagaaatgg cgggaggctg aagaaaaggc cgggaaggaa   6960

ctggacgcca tcatcgcgcc gattacgcct accgctgcgg tacggcatga ccagttccgg   7020

tactatgggt atgcctctgt gatcaacctg ctggatttca cgagcgtggt tgttccggtt   7080

acctttgcgg ataagaacat cgataagaag aatgagagtt tcaaggcggt tagtgagctt   7140

gatgccctcg tgcaggaaga gtatgatccg gaggcgtacc atggggcacc ggttgcagtg   7200

caggttatcg gacggagact cagtgaagag aggacgttgg cgattgcaga ggaagtgggg   7260

aagttgctgg gaaatgtggt gactccatag ctaataagtg tcagatagca atttgcacaa   7320

gaaatcaata ccagcaactg taaataagcg ctgaagtgac catgccatgc tacgaaagag   7380

cagaaaaaaa cctgccgtag aaccgaagag atatgacacg cttccatctc tcaaaggaag   7440

aatcccttca gggttgcgtt tccagtctag acacgtataa cggcacaagt gtctctcacc   7500

aaatgggtta tatctcaaat gtgatctaag gatggaaagc ccagaatatc gatcgcgcgc   7560

atttaaatca gctgcggagc atgagcctat ggcgatcagt ctggtcatgt taaccagcct   7620

gtgctctgac gttaatgcag aatagaaagc cgcggttgca atgcaaatga tgatgccttt   7680

gcagaaatgg cttgctcgct gactgatacc agtaacaact ttgcttggcc gtctagcgct   7740

gttgattgta ttcatcacaa cctcgtctcc ctcctttggg ttgagctctt tggatggctt   7800

tccaaacgtt aatagcgcgt ttttctccac aaagtattcg tatggacgcg cttttgcgtg   7860

tattgcgtga gctaccagca gcccaattgg cgaagtcttg agccgcatcg catagaataa   7920

ttgattgcgc atttgatgcg atttttgagc ggctgtttca ggcgacattt cgcccgccct   7980

tatttgctcc attatatcat cgacggcatg tccaatagcc cggtgatagt cttgtcgaat   8040

atggctgtcg tggataaccc atcggcagca gatgataatg attccgcagc acaagctcgt   8100

atgtgggtag cagaagaact gagcgagatc ttcgagggcg taactctgca tatccgattg   8160

gcctgctgcc acatgtcatt tgcttcggtt tcttttctgt tgagttcttg tatttgggtg   8220

aaagtaacat ggtgtatgac gagagacatt ggtggtaaga aaaaatttca cctcctctta   8280

gtgcaggact gactctcaaa atctatatgc aaatgtgtcg tgtaacaccc ttcgcatgag   8340

cgctgaccgt accctaccat ttcgccccac tcatgatagc agaagagaca tattaattcg   8400

gcaatgctac gaaagtctgc aggtatgctt aaataaacgc ttgccacaga agccgacagt   8460

ttattgttac tacttactat actgtattat tgttgctcac ataaggcggt gaaccattgg   8520

ttcaccacga cgcctgacga ggtaaattac tctctcgtag ggctgccaag gtaggtccca   8580

accccgtatc ctcggtcgag ggtgcgaggt tctttggtcc ttccctcttt ggtaaagccc   8640

agtagcgtgt ttgaatcagt tcacaatctc tcctaaacac agtccgacac taggtaggta   8700

cgttgtaata gcaactcaaa catgtaattc gttcaaggca ggaacatttt ataaacttcc   8760

ctgcgtattt aatcaataaa gatcctagtc caatcgtata ctacctacct acctagctaa   8820

ggtaggtagg tagttcgtgg gaacctggtc gctaattcac gcaacccact ttgcgctctt   8880

cgcctggccg tcgttgaagg taaagcagtt gtacccatca cctaactcaa ccgacacacc   8940

gttgatctgc tcaaggcagt tttcgtcact gtagaattcc acaggttgtt ccacgttgtc   9000

gaattggatc cccctatatt gggcactggc aaacgcggtc gtggacctgg tacagtcgcc   9060

tggctgaaca gtagtagttt cgactacgac gccgccagca caccttccgc cggtatagga   9120

attgaagagt acggggttct gtgcgaagac agccgggcag gcggaaagga tatagaagag   9180

ctgtccagtc acgttagcta gtgaagtaac gtaatggaag gaaagagaaa aggggagcag   9240

ggaggaaact cgtcatttac tcacaacttt gtgcatcttg acaaaagact tctgatatgg   9300

caacctataa ttcaacaaca tgcagcgtag taaagaatag gtgatcttct tgattcagtt   9360

gcttgagggc agggagaatg aagttccttg gaacgattta tatacccttc gcagcaagag   9420

agtcggctta aagaaaggag actgaaagtg tttacgggac gaatatctat ccgattagcg   9480

tagtatcgtc tctacaaggc ggggcgtaaa ttatgttcca aggccggaca acgtgaacaa   9540

caaatggaaa ttccagacgt ttgaggagaa tcaagctcac ttgctcgtgg ataccagtgg   9600

ttatgagcgc caccgctcaa cattgccgcc aatcggataa aaaaaagcct ctagaagagg   9660

agaccagcag ttgttttagg caaaacaatt gtacagagat cggttgtcgt ttgcgagata   9720

ggtaggtatt tacggagtaa cactaaatca aagatacaaa gttttctgcg attattaatt   9780

ctgcgacggt tggcgccatg tggtcttcca gggtgagcaa acgttactct tgctattgac   9840

tattgcaacg acgccgctcg gctgcgacac aacaaagaga cataaggccc tggggaggaa   9900

cgatgtgatc gtcagatcct tcgtagtgaa gatggcgcta cttatgactg catcaagcac   9960

actgtaccga acgcgttaca aaggatcctt tactgacctt cataccaagt ttccaatttg  10020

ttacttgcta aggtcgtgat aatattcatg gtctcctaga ggattgttac agatattaac  10080

agcttgaata gtgtcgagct tataacctgc aaggtacagc caagttgccc agcaccagga  10140

tgttacctcg cttaagttag gcaatagttt gcgagcctaa tgtcgacaaa gtatggcgca  10200

agctgagtac tgccttgggt gaatcctcgc tcaatggtaa ctttgcaagc tcatatgctt  10260

tccaaagctt gtgatacgtg cggttataag ctggcactga cgtgtttcga ggccagatgc  10320

ttgcgaaatc atcaagtgta ttgtggaaag gtctcaggat gaggtcctag aatacgcgag  10380

gcaaatttgt ctgatcgtct ttcaataacc tcatagtcga gtcacaaatg ttggaggtct  10440

ggttcaagcc gagccaagca atagcttggt cgggcgcgtc acagcatcag gaatgctaac  10500

gcttgcacat ctcgcggact ttattatgcc tggacgcaaa tattgatacc agaatcaagc  10560

cacaccctgt gaagcgtaac ttgtttttct ctgctttctt aaaaagctgc gtatatcatt  10620

gctagagcgc ccgtgaacaa cggaactcat tgtctcttta tcttcttact cgcccgggca  10680

agggcgaatt ccagcacact ggcggccgtt actagtggat ccgagctcgg taccaagctt  10740

gatgcatagc ttgagtattc taacgcgtca cctaaatagc ttggcgtaat catggtcata  10800

gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag  10860

cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg  10920

ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca  10980

acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc  11040

gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg  11100

gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa  11160

gcccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga  11220

cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag  11280

ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct  11340

taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg  11400

ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc  11460

ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt  11520

aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta  11580

tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac  11640

agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc  11700

ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat  11760

tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc  11820

tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt  11880

cacctagatc cttttaaatt aaaaatgaag ttttagcacg tgtcagtcct gctcctcggc  11940

cacgaagtgc acgcagttgc cggccgggtc gcgcagggcg aactcccgcc cccacggctg  12000

ctcgccgatc tcggtcatgg ccggcccgga ggcgtcccgg aagttcgtgg acacgacctc  12060

cgaccactcg gcgtacagct cgtccaggcc gcgcacccac acccaggcca gggtgttgtc  12120

cggcaccacc tggtcctgga ccgcgctgat gaacagggtc acgtcgtccc ggaccacacc  12180

ggcgaagtcg tcctccacga agtcccggga gaacccgagc cggtcggtcc agaactcgac  12240

cgctccggcg acgtcgcgcg cggtgagcac cggaacggca ctggtcaact tggccatggt  12300

ggccctcctc acgtgctatt attgaagcat ttatcagggt tattgtctca tgagcggata  12360

catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa  12420

agtgccacct gtatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc  12480

aggaaattgt aagcgttaat aattcagaag aactcgtcaa gaaggcgata gaaggcgatg  12540

cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg  12600

ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca  12660

cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc  12720

aagcaggcat cgccatgggt cacgacgaga tcctcgccgt cgggcatgct cgccttgagc  12780

ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg  12840

acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg  12900

aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat  12960

actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat  13020

agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc  13080

gtcgtggcca gccacgatag ccgcgctgcc tcgtcttgca gttcattcag ggcaccggac  13140

aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca  13200

tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg  13260

gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc  13320

tcttgatcag agcttgatcc cctgcgccat cagatccttg gcggcgagaa agccatccag  13380

tttactttgc agggcttccc aaccttacca gagggcgccc cagctggcaa ttccggttcg  13440

cttgctgtcc ataaaaccgc ccagtctagc tatcgccatg taagcccact gcaagctacc  13500

tgctttctct ttgcgcttgc gttttccctt gtccagatag cccagtagct gacattcatc  13560

cggggtcagc accgtttctg cggactggct ttctacgtga aaaggatcta ggtgaagatc  13620

ctttttgata atctcatgcc tgacatttat attccccaga acatcaggtt aatggcgttt  13680

ttgatgtcat tttcgcggtg gctgagatca gccacttctt ccccgataac ggagaccggc  13740

acactggcca tatcggtggt catcatgcgc cagctttcat ccccgatatg caccaccggg  13800

taaagttcac gggagacttt atctgacagc agacgtgcac tggccagggg gatcaccatc  13860

cgtcgccccg gcgtgtcaat aatatcactc tgtacatcca caaacagacg ataacggctc  13920

tctcttttat aggtgtaaac cttaaactgc cgtacgtata ggctgcgcaa ctgttgggaa  13980

gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca  14040

aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc  14100

agtgaattgt aatacgactc actatagggc gaattgggcc ctctagatgc atgctcga    14158


<210>  14
<211>  11312
<212>  DNA
<213>  Artificial

<220>
<223>  pTrex4:CBH1-E1 expression vector

<400>  14
aagcttaact agtacttctc gagctctgta catgtccggt cgcgacgtac gcgtatcgat     60

ggcgccagct gcaggcggcc gcctgcagcc acttgcagtc ccgtggaatt ctcacggtga    120

atgtaggcct tttgtagggt aggaattgtc actcaagcac ccccaacctc cattacgcct    180

cccccataga gttcccaatc agtgagtcat ggcactgttc tcaaatagat tggggagaag    240

ttgacttccg cccagagctg aaggtcgcac aaccgcatga tatagggtcg gcaacggcaa    300

aaaagcacgt ggctcaccga aaagcaagat gtttgcgatc taacatccag gaacctggat    360

acatccatca tcacgcacga ccactttgat ctgctggtaa actcgtattc gccctaaacc    420

gaagtgacgt ggtaaatcta cacgtgggcc cctttcggta tactgcgtgt gtcttctcta    480

ggtgccattc ttttcccttc ctctagtgtt gaattgtttg tgttggagtc cgagctgtaa    540

ctacctctga atctctggag aatggtggac taacgactac cgtgcacctg catcatgtat    600

ataatagtga tcctgagaag gggggtttgg agcaatgtgg gactttgatg gtcatcaaac    660

aaagaacgaa gacgcctctt ttgcaaagtt ttgtttcggc tacggtgaag aactggatac    720

ttgttgtgtc ttctgtgtat ttttgtggca acaagaggcc agagacaatc tattcaaaca    780

ccaagcttgc tcttttgagc tacaagaacc tgtggggtat atatctagag ttgtgaagtc    840

ggtaatcccg ctgtatagta atacgagtcg catctaaata ctccgaagct gctgcgaacc    900

cggagaatcg agatgtgctg gaaagcttct agcgagcggc taaattagca tgaaaggcta    960

tgagaaattc tggagacggc ttgttgaatc atggcgttcc attcttcgac aagcaaagcg   1020

ttccgtcgca gtagcaggca ctcattcccg aaaaaactcg gagattccta agtagcgatg   1080

gaaccggaat aatataatag gcaatacatt gagttgcctc gacggttgca atgcaggggt   1140

actgagcttg gacataactg ttccgtaccc cacctcttct caacctttgg cgtttccctg   1200

attcagcgta cccgtacaag tcgtaatcac tattaaccca gactgaccgg acgtgttttg   1260

cccttcattt ggagaaataa tgtcattgcg atgtgtaatt tgcctgcttg accgactggg   1320

gctgttcgaa gcccgaatgt aggattgtta tccgaactct gctcgtagag gcatgttgtg   1380

aatctgtgtc gggcaggaca cgcctcgaag gttcacggca agggaaacca ccgatagcag   1440

tgtctagtag caacctgtaa agccgcaatg cagcatcact ggaaaataca aaccaatggc   1500

taaaagtaca taagttaatg cctaaagaag tcatatacca gcggctaata attgtacaat   1560

caagtggcta aacgtaccgt aatttgccaa cggcttgtgg ggttgcagaa gcaacggcaa   1620

agccccactt ccccacgttt gtttcttcac tcagtccaat ctcagctggt gatcccccaa   1680

ttgggtcgct tgtttgttcc ggtgaagtga aagaagacag aggtaagaat gtctgactcg   1740

gagcgttttg catacaacca agggcagtga tggaagacag tgaaatgttg acattcaagg   1800

agtatttagc cagggatgct tgagtgtatc gtgtaaggag gtttgtctgc cgatacgacg   1860

aatactgtat agtcacttct gatgaagtgg tccatattga aatgtaagtc ggcactgaac   1920

aggcaaaaga ttgagttgaa actgcctaag atctcgggcc ctcgggcctt cggcctttgg   1980

gtgtacatgt ttgtgctccg ggcaaatgca aagtgtggta ggatcgaaca cactgctgcc   2040

tttaccaagc agctgagggt atgtgatagg caaatgttca ggggccactg catggtttcg   2100

aatagaaaga gaagcttagc caagaacaat agccgataaa gatagcctca ttaaacggaa   2160

tgagctagta ggcaaagtca gcgaatgtgt atatataaag gttcgaggtc cgtgcctccc   2220

tcatgctctc cccatctact catcaactca gatcctccag gagacttgta caccatcttt   2280

tgaggcacag aaacccaata gtcaaccgcg gactgcgcat catgtatcgg aagttggccg   2340

tcatctcggc cttcttggcc acagctcgtg ctcagtcggc ctgcactctc caatcggaga   2400

ctcacccgcc tctgacatgg cagaaatgct cgtctggtgg cacttgcact caacagacag   2460

gctccgtggt catcgacgcc aactggcgct ggactcacgc tacgaacagc agcacgaact   2520

gctacgatgg caacacttgg agctcgaccc tatgtcctga caacgagacc tgcgcgaaga   2580

actgctgtct ggacggtgcc gcctacgcgt ccacgtacgg agttaccacg agcggtaaca   2640

gcctctccat tggctttgtc acccagtctg cgcagaagaa cgttggcgct cgcctttacc   2700

ttatggcgag cgacacgacc taccaggaat tcaccctgct tggcaacgag ttctctttcg   2760

atgttgatgt ttcgcagctg ccgtaagtga cttaccatga acccctgacg tatcttcttg   2820

tgggctccca gctgactggc caatttaagg tgcggcttga acggagctct ctacttcgtg   2880

tccatggacg cggatggtgg cgtgagcaag tatcccacca acaccgctgg cgccaagtac   2940

ggcacggggt actgtgacag ccagtgtccc cgcgatctga agttcatcaa tggccaggcc   3000

aacgttgagg gctgggagcc gtcatccaac aacgcaaaca cgggcattgg aggacacgga   3060

agctgctgct ctgagatgga tatctgggag gccaactcca tctccgaggc tcttaccccc   3120

cacccttgca cgactgtcgg ccaggagatc tgcgagggtg atgggtgcgg cggaacttac   3180

tccgataaca gatatggcgg cacttgcgat cccgatggct gcgactggaa cccataccgc   3240

ctgggcaaca ccagcttcta cggccctggc tcaagcttta ccctcgatac caccaagaaa   3300

ttgaccgttg tcacccagtt cgagacgtcg ggtgccatca accgatacta tgtccagaat   3360

ggcgtcactt tccagcagcc caacgccgag cttggtagtt actctggcaa cgagctcaac   3420

gatgattact gcacagctga ggaggcagaa ttcggcggat cctctttctc agacaagggc   3480

ggcctgactc agttcaagaa ggctacctct ggcggcatgg ttctggtcat gagtctgtgg   3540

gatgatgtga gtttgatgga caaacatgcg cgttgacaaa gagtcaagca gctgactgag   3600

atgttacagt actacgccaa catgctgtgg ctggactcca cctacccgac aaacgagacc   3660

tcctccacac ccggtgccgt gcgcggaagc tgctccacca gctccggtgt ccctgctcag   3720

gtcgaatctc agtctcccaa cgccaaggtc accttctcca acatcaagtt cggacccatt   3780

ggcagcaccg gcaaccctag cggcggcaac cctcccggcg gaaacccgcc tggcaccacc   3840

accacccgcc gcccagccac taccactgga agctctcccg gacctactag taagcgggcg   3900

ggcggcggct attggcacac gagcggccgg gagatcctgg acgcgaacaa cgtgccggta   3960

cggatcgccg gcatcaactg gtttgggttc gaaacctgca attacgtcgt gcacggtctc   4020

tggtcacgcg actaccgcag catgctcgac cagataaagt cgctcggcta caacacaatc   4080

cggctgccgt actctgacga cattctcaag ccgggcacca tgccgaacag catcaatttt   4140

taccagatga atcaggacct gcagggtctg acgtccttgc aggtcatgga caaaatcgtc   4200

gcgtacgccg gtcagatcgg cctgcgcatc attcttgacc gccaccgacc ggattgcagc   4260

gggcagtcgg cgctgtggta cacgagcagc gtctcggagg ctacgtggat ttccgacctg   4320

caagcgctgg cgcagcgcta caagggaaac ccgacggtcg tcggctttga cttgcacaac   4380

gagccgcatg acccggcctg ctggggctgc ggcgatccga gcatcgactg gcgattggcc   4440

gccgagcggg ccggaaacgc cgtgctctcg gtgaatccga acctgctcat tttcgtcgaa   4500

ggtgtgcaga gctacaacgg agactcctac tggtggggcg gcaacctgca aggagccggc   4560

cagtacccgg tcgtgctgaa cgtgccgaac cgcctggtgt actcggcgca cgactacgcg   4620

acgagcgtct acccgcagac gtggttcagc gatccgacct tccccaacaa catgcccggc   4680

atctggaaca agaactgggg atacctcttc aatcagaaca ttgcaccggt atggctgggc   4740

gaattcggta cgacactgca atccacgacc gaccagacgt ggctgaagac gctcgtccag   4800

tacctacggc cgaccgcgca atacggtgcg gacagcttcc agtggacctt ctggtcctgg   4860

aaccccgatt ccggcgacac aggaggaatt ctcaaggatg actggcagac ggtcgacaca   4920

gtaaaagacg gctatctcgc gccgatcaag tcgtcgattt tcgatcctgt ctaaggcgcg   4980

ccgcgcgcca gctccgtgcg aaagcctgac gcaccggtag attcttggtg agcccgtatc   5040

atgacggcgg cgggagctac atggccccgg gtgatttatt ttttttgtat ctacttctga   5100

cccttttcaa atatacggtc aactcatctt tcactggaga tgcggcctgc ttggtattgc   5160

gatgttgtca gcttggcaaa ttgtggcttt cgaaaacaca aaacgattcc ttagtagcca   5220

tgcattttaa gataacggaa tagaagaaag aggaaattaa aaaaaaaaaa aaaacaaaca   5280

tcccgttcat aacccgtaga atcgccgctc ttcgtgtatc ccagtaccag tttattttga   5340

atagctcgcc cgctggagag catcctgaat gcaagtaaca accgtagagg ctgacacggc   5400

aggtgttgct agggagcgtc gtgttctaca aggccagacg tcttcgcggt tgatatatat   5460

gtatgtttga ctgcaggctg ctcagcgacg acagtcaagt tcgccctcgc tgcttgtgca   5520

ataatcgcag tggggaagcc acaccgtgac tcccatcttt cagtaaagct ctgttggtgt   5580

ttatcagcaa tacacgtaat ttaaactcgt tagcatgggg ctgatagctt aattaccgtt   5640

taccagtgcc gcggttctgc agctttcctt ggcccgtaaa attcggcgaa gccagccaat   5700

caccagctag gcaccagcta aaccctataa ttagtctctt atcaacacca tccgctcccc   5760

cgggatcaat gaggagaatg agggggatgc ggggctaaag aagcctacat aaccctcatg   5820

ccaactccca gtttacactc gtcgagccaa catcctgact ataagctaac acagaatgcc   5880

tcaatcctgg gaagaactgg ccgctgataa gcgcgcccgc ctcgcaaaaa ccatccctga   5940

tgaatggaaa gtccagacgc tgcctgcgga agacagcgtt attgatttcc caaagaaatc   6000

ggggatcctt tcagaggccg aactgaagat cacagaggcc tccgctgcag atcttgtgtc   6060

caagctggcg gccggagagt tgacctcggt ggaagttacg ctagcattct gtaaacgggc   6120

agcaatcgcc cagcagttag tagggtcccc tctacctctc agggagatgt aacaacgcca   6180

ccttatggga ctatcaagct gacgctggct tctgtgcaga caaactgcgc ccacgagttc   6240

ttccctgacg ccgctctcgc gcaggcaagg gaactcgatg aatactacgc aaagcacaag   6300

agacccgttg gtccactcca tggcctcccc atctctctca aagaccagct tcgagtcaag   6360

gtacaccgtt gcccctaagt cgttagatgt ccctttttgt cagctaacat atgccaccag   6420

ggctacgaaa catcaatggg ctacatctca tggctaaaca agtacgacga aggggactcg   6480

gttctgacaa ccatgctccg caaagccggt gccgtcttct acgtcaagac ctctgtcccg   6540

cagaccctga tggtctgcga gacagtcaac aacatcatcg ggcgcaccgt caacccacgc   6600

aacaagaact ggtcgtgcgg cggcagttct ggtggtgagg gtgcgatcgt tgggattcgt   6660

ggtggcgtca tcggtgtagg aacggatatc ggtggctcga ttcgagtgcc ggccgcgttc   6720

aacttcctgt acggtctaag gccgagtcat gggcggctgc cgtatgcaaa gatggcgaac   6780

agcatggagg gtcaggagac ggtgcacagc gttgtcgggc cgattacgca ctctgttgag   6840

ggtgagtcct tcgcctcttc cttcttttcc tgctctatac caggcctcca ctgtcctcct   6900

ttcttgcttt ttatactata tacgagaccg gcagtcactg atgaagtatg ttagacctcc   6960

gcctcttcac caaatccgtc ctcggtcagg agccatggaa atacgactcc aaggtcatcc   7020

ccatgccctg gcgccagtcc gagtcggaca ttattgcctc caagatcaag aacggcgggc   7080

tcaatatcgg ctactacaac ttcgacggca atgtccttcc acaccctcct atcctgcgcg   7140

gcgtggaaac caccgtcgcc gcactcgcca aagccggtca caccgtgacc ccgtggacgc   7200

catacaagca cgatttcggc cacgatctca tctcccatat ctacgcggct gacggcagcg   7260

ccgacgtaat gcgcgatatc agtgcatccg gcgagccggc gattccaaat atcaaagacc   7320

tactgaaccc gaacatcaaa gctgttaaca tgaacgagct ctgggacacg catctccaga   7380

agtggaatta ccagatggag taccttgaga aatggcggga ggctgaagaa aaggccggga   7440

aggaactgga cgccatcatc gcgccgatta cgcctaccgc tgcggtacgg catgaccagt   7500

tccggtacta tgggtatgcc tctgtgatca acctgctgga tttcacgagc gtggttgttc   7560

cggttacctt tgcggataag aacatcgata agaagaatga gagtttcaag gcggttagtg   7620

agcttgatgc cctcgtgcag gaagagtatg atccggaggc gtaccatggg gcaccggttg   7680

cagtgcaggt tatcggacgg agactcagtg aagagaggac gttggcgatt gcagaggaag   7740

tggggaagtt gctgggaaat gtggtgactc catagctaat aagtgtcaga tagcaatttg   7800

cacaagaaat caataccagc aactgtaaat aagcgctgaa gtgaccatgc catgctacga   7860

aagagcagaa aaaaacctgc cgtagaaccg aagagatatg acacgcttcc atctctcaaa   7920

ggaagaatcc cttcagggtt gcgtttccag tctagacacg tataacggca caagtgtctc   7980

tcaccaaatg ggttatatct caaatgtgat ctaaggatgg aaagcccaga atctaggcct   8040

attaatattc cggagtatac gtagccggct aacgttaaca accggtacct ctagaactat   8100

agctagcatg cgcaaattta aagcgctgat atcgatcgcg cgcagatcca tatatagggc   8160

ccgggttata attacctcag gtcgacgtcc catggccatt cgaattcgta atcatggtca   8220

tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga   8280

agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg   8340

cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc   8400

caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac   8460

tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata   8520

cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa   8580

aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct   8640

gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa   8700

agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg   8760

cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca   8820

cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa   8880

ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg   8940

gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg   9000

tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga   9060

acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc   9120

tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag   9180

attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac   9240

gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc   9300

ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag   9360

taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt   9420

ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag   9480

ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca   9540

gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact   9600

ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca   9660

gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg   9720

tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc   9780

atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg   9840

gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca   9900

tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt   9960

atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc  10020

agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc  10080

ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca  10140

tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa  10200

aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat  10260

tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa  10320

aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa  10380

accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc  10440

gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca  10500

gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt  10560

ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac  10620

cataaaattg taaacgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc  10680

tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagccc  10740

gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac  10800

tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca  10860

cccaaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg  10920

agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag  10980

aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc  11040

accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt actatggttg ctttgacgta  11100

tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc  11160

cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc  11220

agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc  11280

agtcacgacg ttgtaaaacg acggccagtg cc                                11312


