                         SEQUENCE LISTING

<110>  Genmab A/S
 
<120>  STABLE IgG4 ANTIBODIES

<130>  P/44.WO

<160>  49    

<170>  PatentIn version 3.2

<210>  1
<211>  36
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  1
agccaccgta cgtttgattt ccagcttggt gcctcc                               36


<210>  2
<211>  44
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  2
gatgcaagct tgccgccacc atggagtcac agattcaggc attt                      44


<210>  3
<211>  42
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  3
cgatgggccc ttggtgctgg ctgaggagac ggtgactgag gt                        42


<210>  4
<211>  44
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  4
gatgcaagct tgccgccacc atgaaatgca gctgggttat cttc                      44


<210>  5
<211>  36
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  5
agccaccgta cgttttattt ccaactttgt ccccga                               36


<210>  6
<211>  44
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  6
gatgcaagct tgccgccacc atggaatcac agactcaggt cctc                      44


<210>  7
<211>  42
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  7
cgatgggccc ttggtgctgg ctgcagagaa agtgaccaga gt                        42


<210>  8
<211>  44
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  8
gatgcaagct tgccgccacc atgggatgga gctatatcat cctc                      44


<210>  9
<211>  32
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  9
tgagaattcg gtgggtgctt tatttccatg ct                                   32


<210>  10
<211>  33
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  10
gtagaagctt accatcgcgg atagacaaga acc                                  33


<210>  11
<211>  26
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  11
tgttaactgc tcactggatg gtggga                                          26


<210>  12
<211>  27
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  12
tccctgggca caattttctt gtccacc                                         27


<210>  13
<211>  31
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  13
tgaaagcttc taatacgact cactataggg c                                    31


<210>  14
<211>  54
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  14
tgaaagcttc taatacgact cactataggg caagcagtgg tatcaacgca gagt           54


<210>  15
<211>  137
<212>  PRT
<213>  artificial

<220>
<223>  variable region sequence of antibody

<400>  15

Met Lys Cys Ser Trp Val Ile Phe Phe Leu Met Ala Val Val Thr Gly 
1               5                   10                  15      


Val Asn Ser Glu Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Lys 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Leu Ser Cys Thr Ala Ser Gly Phe Asn Ile 
        35                  40                  45              


Lys Asp Thr Tyr Ile His Trp Val Lys Gln Arg Pro Glu Gln Gly Leu 
    50                  55                  60                  


Glu Trp Val Gly Arg Ile Asp Pro Ala Thr Gly Asn Thr Arg Tyr Asp 
65                  70                  75                  80  


Pro Lys Phe Gln Gly Lys Ala Thr Ile Thr Ala Asp Thr Ser Ser Asn 
                85                  90                  95      


Thr Ala Tyr Leu Gln Leu Ser Ser Leu Thr Ser Glu Asp Thr Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ala Ser Phe Arg Pro Gly Tyr Ala Leu Asp Tyr Trp Gly 
        115                 120                 125             


Gln Gly Thr Ser Val Thr Val Ser Ser 
    130                 135         


<210>  16
<211>  127
<212>  PRT
<213>  artificial

<220>
<223>  variable region sequence of antibody

<400>  16

Met Glu Ser Gln Ile Gln Ala Phe Val Phe Val Phe Leu Trp Leu Ser 
1               5                   10                  15      


Gly Val Asp Gly Asp Ile Val Met Thr Gln Ser His Lys Phe Met Ser 
            20                  25                  30          


Thr Ser Val Gly Asp Arg Val Ser Phe Thr Cys Lys Ala Ser Gln Asp 
        35                  40                  45              


Val Phe Thr Ala Val Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro 
    50                  55                  60                  


Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Arg Thr Gly Val Pro Asp 
65                  70                  75                  80  


Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Tyr Thr Leu Thr Ile Ser 
                85                  90                  95      


Ser Val Gln Ala Glu Asp Leu Ala Leu Tyr Tyr Cys Gln Gln His Phe 
            100                 105                 110         


Ser Thr Pro Pro Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
        115                 120                 125         


<210>  17
<211>  138
<212>  PRT
<213>  artificial

<220>
<223>  variable region sequence of antibody

<400>  17

Met Gly Trp Ser Tyr Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Asp 
1               5                   10                  15      


Val His Ser Gln Val Gln Leu Gln Gln Pro Gly Ala Glu Leu Val Lys 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Ser Phe 
        35                  40                  45              


Thr Ser Tyr Trp Met His Trp Leu Lys Gln Arg Pro Gly Gln Gly Leu 
    50                  55                  60                  


Glu Trp Ile Gly Glu Ile Asn Pro Asn Asn Gly Arg Thr Tyr Tyr Asn 
65                  70                  75                  80  


Glu Lys Phe Lys Thr Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser 
                85                  90                  95      


Thr Ala Tyr Met Gln Leu Asn Ser Leu Thr Ser Glu Asp Ser Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ala Arg Arg Leu Thr Met Val Glu Ser Phe Ala Tyr Trp 
        115                 120                 125             


Gly Gln Gly Thr Leu Val Thr Phe Ser Ala 
    130                 135             


<210>  18
<211>  133
<212>  PRT
<213>  artificial

<220>
<223>  variable region sequence of antibody

<400>  18

Met Glu Ser Gln Thr Gln Val Leu Met Ser Leu Leu Phe Trp Val Ser 
1               5                   10                  15      


Gly Thr Cys Gly Asp Ile Val Met Thr Gln Ser Pro Ser Ser Leu Thr 
            20                  25                  30          


Val Thr Ala Gly Glu Lys Val Thr Met Ser Cys Lys Ser Ser Gln Ser 
        35                  40                  45              


Leu Leu Asn Ser Gly Asn Gln Lys Asn Tyr Leu Thr Trp Tyr Gln Gln 
    50                  55                  60                  


Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg 
65                  70                  75                  80  


Glu Ser Gly Val Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp 
                85                  90                  95      


Phe Ser Leu Thr Ile Ser Ser Val Gln Ala Glu Asp Leu Ala Ile Tyr 
            100                 105                 110         


Tyr Cys Gln Asn Asp Tyr Ser Tyr Pro Phe Thr Phe Gly Ser Gly Thr 
        115                 120                 125             


Lys Leu Glu Ile Lys 
    130             


<210>  19
<211>  45
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  19
cttgtgacaa aactcacacc tgcccatcgt gcccaggtaa gccag                     45


<210>  20
<211>  45
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  20
ctggcttacc tgggcacgat gggcaggtgt gagttttgtc acaag                     45


<210>  21
<211>  43
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  21
cccccatgcc caccatgccc aggtaagcca acccaggcct cgc                       43


<210>  22
<211>  43
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  22
gcgaggcctg ggttggctta cctgggcatg gtgggcatgg ggg                       43


<210>  23
<211>  45
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  23
cttgtgacaa aactcacacc tgcccatcgt gcccaggtaa gccag                     45


<210>  24
<211>  45
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  24
ctggcttacc tgggcacgat gggcaggtgt gagttttgtc acaag                     45


<210>  25
<211>  45
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  25
ccttcttcct ctatagcagg ctcaccgtag acaagagcag gtggc                     45


<210>  26
<211>  45
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  26
gccacctgct cttgtctacg gtgagcctgc tatagaggaa gaagg                     45


<210>  27
<211>  45
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  27
ggctccttct tcctctacag caagctaacc gtagacaaga gcagg                     45


<210>  28
<211>  45
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  28
cctgctcttg tctacggtta gcttgctgta gaggaagaag gagcc                     45


<210>  29
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  29
tctcctcagc cagcaccaag                                                 20


<210>  30
<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  30
gtttgtctgc agccagcacc aag                                             23


<210>  31
<211>  25
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  31
catctccaaa gccaaaggtg ggacc                                           25


<210>  32
<211>  25
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  32
ggtcccacct ttggctttgg agatg                                           25


<210>  33
<211>  24
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  33
cgacggctcc ttcttcctct acag                                            24


<210>  34
<211>  24
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  34
ctgtagagga agaaggagcc gtcg                                            24


<210>  35
<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  35
caagagccat atccgggagg acc                                             23


<210>  36
<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  36
ggtcctcccg gatatggctc ttg                                             23


<210>  37
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  37
gtcagaggta actcccgttg                                                 20


<210>  38
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  38
gttgtggttt gtccaaactc                                                 20


<210>  39
<211>  327
<212>  PRT
<213>  homo sapiens

<400>  39

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg 
1               5                   10                  15      


Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr 
65                  70                  75                  80  


Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser Cys Pro Ala Pro 
            100                 105                 110         


Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
        115                 120                 125             


Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
    130                 135                 140                 


Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp 
145                 150                 155                 160 


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe 
                165                 170                 175     


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
            180                 185                 190         


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu 
        195                 200                 205             


Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
    210                 215                 220                 


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys 
225                 230                 235                 240 


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
                245                 250                 255     


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
            260                 265                 270         


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
        275                 280                 285             


Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser 
    290                 295                 300                 


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
305                 310                 315                 320 


Leu Ser Leu Ser Leu Gly Lys 
                325         


<210>  40
<211>  327
<212>  PRT
<213>  homo sapiens

<400>  40

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg 
1               5                   10                  15      


Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr 
65                  70                  75                  80  


Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser Cys Pro Ala Pro 
            100                 105                 110         


Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
        115                 120                 125             


Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
    130                 135                 140                 


Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp 
145                 150                 155                 160 


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe 
                165                 170                 175     


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
            180                 185                 190         


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu 
        195                 200                 205             


Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
    210                 215                 220                 


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys 
225                 230                 235                 240 


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
                245                 250                 255     


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
            260                 265                 270         


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
        275                 280                 285             


Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser 
    290                 295                 300                 


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
305                 310                 315                 320 


Leu Ser Leu Ser Leu Gly Lys 
                325         


<210>  41
<211>  327
<212>  PRT
<213>  homo sapiens

<400>  41

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg 
1               5                   10                  15      


Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr 
65                  70                  75                  80  


Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser Cys Pro Ala Pro 
            100                 105                 110         


Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
        115                 120                 125             


Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
    130                 135                 140                 


Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp 
145                 150                 155                 160 


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe 
                165                 170                 175     


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Val His Gln Asp 
            180                 185                 190         


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu 
        195                 200                 205             


Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
    210                 215                 220                 


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys 
225                 230                 235                 240 


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
                245                 250                 255     


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
            260                 265                 270         


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
        275                 280                 285             


Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser 
    290                 295                 300                 


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
305                 310                 315                 320 


Leu Ser Leu Ser Leu Gly Lys 
                325         


<210>  42
<211>  48
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  42
ccagtgctgg acagcgacgg aagcttcgcc ctgtacagca ggctgacc                  48


<210>  43
<211>  48
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  43
ggtcagcctg ctgtacaggg cgaagcttcc gtcgctgtcc agcactgg                  48


<210>  44
<211>  51
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  44
ccagtgctgg acagcgacgg atccttctta ctgtacagca ggctgaccgt g              51


<210>  45
<211>  51
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  45
cacggtcagc ctgctgtaca gtaagaagga tccgtcgctg tccagcactg g              51


<210>  46
<211>  46
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  46
gctccttctt cctgtacagc gcgttaaccg tggacaagtc caggtg                    46


<210>  47
<211>  46
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  47
cacctggact tgtccacggt taacgcgctg tacaggaaga aggagc                    46


<210>  48
<211>  45
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  48
ctccttcttc ctgtacagca agcttaccgt ggacaagtcc aggtg                     45


<210>  49
<211>  45
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  49
cacctggact tgtccacggt aagcttgctg tacaggaaga aggag                     45


