                         SEQUENCE LISTING

<110>  Plant Research International B.V.
 
<120>  Alg3 mutant

<130>  P80679PC00

<150>  EP 07108728.2
<151>  2007-05-23

<160>  25    

<170>  PatentIn version 3.3

<210>  1
<211>  438
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Ala Ala Gly Leu Arg Lys Arg Gly Arg Ser Gly Ser Ala Ala Gln 
1               5                   10                  15      


Ala Glu Gly Leu Cys Lys Gln Trp Leu Gln Arg Ala Trp Gln Glu Arg 
            20                  25                  30          


Arg Leu Leu Leu Arg Glu Pro Arg Tyr Thr Leu Leu Val Ala Ala Cys 
        35                  40                  45              


Leu Cys Leu Ala Glu Val Gly Ile Thr Phe Trp Val Ile His Arg Val 
    50                  55                  60                  


Ala Tyr Thr Glu Ile Asp Trp Lys Ala Tyr Met Ala Glu Val Glu Gly 
65                  70                  75                  80  


Val Ile Asn Gly Thr Tyr Asp Tyr Thr Gln Leu Gln Gly Asp Thr Gly 
                85                  90                  95      


Pro Leu Val Tyr Pro Ala Gly Phe Val Tyr Ile Phe Met Gly Leu Tyr 
            100                 105                 110         


Tyr Ala Thr Ser Arg Gly Thr Asp Ile Arg Met Ala Gln Asn Ile Phe 
        115                 120                 125             


Ala Val Leu Tyr Leu Ala Thr Leu Leu Leu Val Phe Leu Ile Tyr His 
    130                 135                 140                 


Gln Thr Cys Lys Val Pro Pro Phe Val Phe Phe Phe Met Cys Cys Ala 
145                 150                 155                 160 


Ser Tyr Arg Val His Ser Ile Phe Val Leu Arg Leu Phe Asn Asp Pro 
                165                 170                 175     


Val Ala Met Val Leu Leu Phe Leu Ser Ile Asn Leu Leu Leu Ala Gln 
            180                 185                 190         


Arg Trp Gly Trp Gly Cys Cys Phe Phe Ser Leu Ala Val Ser Val Lys 
        195                 200                 205             


Met Asn Val Leu Leu Phe Ala Pro Gly Leu Leu Phe Leu Leu Leu Thr 
    210                 215                 220                 


Gln Phe Gly Phe Arg Gly Ala Leu Pro Lys Leu Gly Ile Cys Ala Gly 
225                 230                 235                 240 


Leu Gln Val Val Leu Gly Leu Pro Phe Leu Leu Glu Asn Pro Ser Gly 
                245                 250                 255     


Tyr Leu Ser Arg Ser Phe Asp Leu Gly Arg Gln Phe Leu Phe His Trp 
            260                 265                 270         


Thr Val Asn Trp Arg Phe Leu Pro Glu Ala Leu Phe Leu His Arg Ala 
        275                 280                 285             


Phe His Leu Ala Leu Leu Thr Ala His Leu Thr Leu Leu Leu Leu Phe 
    290                 295                 300                 


Ala Leu Cys Arg Trp His Arg Thr Gly Glu Ser Ile Leu Ser Leu Leu 
305                 310                 315                 320 


Arg Asp Pro Ser Lys Arg Lys Val Pro Pro Gln Pro Leu Thr Pro Asn 
                325                 330                 335     


Gln Ile Val Ser Thr Leu Phe Thr Ser Asn Phe Ile Gly Ile Cys Phe 
            340                 345                 350         


Ser Arg Ser Leu His Tyr Gln Phe Tyr Val Trp Tyr Phe His Thr Leu 
        355                 360                 365             


Pro Tyr Leu Leu Trp Ala Met Pro Ala Arg Trp Leu Thr His Leu Leu 
    370                 375                 380                 


Arg Leu Leu Val Leu Gly Leu Ile Glu Leu Ser Trp Asn Thr Tyr Pro 
385                 390                 395                 400 


Ser Thr Ser Cys Ser Ser Ala Ala Leu His Ile Cys His Ala Val Ile 
                405                 410                 415     


Leu Leu Gln Leu Trp Leu Gly Pro Gln Pro Phe Pro Lys Ser Thr Gln 
            420                 425                 430         


His Ser Lys Lys Ala His 
        435             


<210>  2
<211>  458
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  2

Met Glu Gly Glu Gln Ser Pro Gln Gly Glu Lys Ser Leu Gln Arg Lys 
1               5                   10                  15      


Gln Phe Val Arg Pro Pro Leu Asp Leu Trp Gln Asp Leu Lys Asp Gly 
            20                  25                  30          


Val Arg Tyr Val Ile Phe Asp Cys Arg Ala Asn Leu Ile Val Met Pro 
        35                  40                  45              


Leu Leu Ile Leu Phe Glu Ser Met Leu Cys Lys Ile Ile Ile Lys Lys 
    50                  55                  60                  


Val Ala Tyr Thr Glu Ile Asp Tyr Lys Ala Tyr Met Glu Gln Ile Glu 
65                  70                  75                  80  


Met Ile Gln Leu Asp Gly Met Leu Asp Tyr Ser Gln Val Ser Gly Gly 
                85                  90                  95      


Thr Gly Pro Leu Val Tyr Pro Ala Gly His Val Leu Ile Tyr Lys Met 
            100                 105                 110         


Met Tyr Trp Leu Thr Glu Gly Met Asp His Val Glu Arg Gly Gln Val 
        115                 120                 125             


Phe Phe Arg Tyr Leu Tyr Leu Leu Thr Leu Ala Leu Gln Met Ala Cys 
    130                 135                 140                 


Tyr Tyr Leu Leu His Leu Pro Pro Trp Cys Val Val Leu Ala Cys Leu 
145                 150                 155                 160 


Ser Lys Arg Leu His Ser Ile Tyr Val Leu Arg Leu Phe Asn Asp Cys 
                165                 170                 175     


Phe Thr Thr Leu Phe Met Val Val Thr Val Leu Gly Ala Ile Val Ala 
            180                 185                 190         


Ser Arg Cys His Gln Arg Pro Lys Leu Lys Lys Ser Leu Ala Leu Val 
        195                 200                 205             


Ile Ser Ala Thr Tyr Ser Met Ala Val Ser Ile Lys Met Asn Ala Leu 
    210                 215                 220                 


Leu Tyr Phe Pro Ala Met Met Ile Ser Leu Phe Ile Leu Asn Asp Ala 
225                 230                 235                 240 


Asn Val Ile Leu Thr Leu Leu Asp Leu Val Ala Met Ile Ala Trp Gln 
                245                 250                 255     


Val Ala Val Ala Val Pro Phe Leu Arg Ser Phe Pro Gln Gln Tyr Leu 
            260                 265                 270         


His Cys Ala Phe Asn Phe Gly Arg Lys Phe Met Tyr Gln Trp Ser Ile 
        275                 280                 285             


Asn Trp Gln Met Met Asp Glu Glu Ala Phe Asn Asp Lys Arg Phe His 
    290                 295                 300                 


Leu Ala Leu Leu Ile Ser His Leu Ile Ala Leu Thr Thr Leu Phe Val 
305                 310                 315                 320 


Thr Arg Tyr Pro Arg Ile Leu Pro Asp Leu Trp Ser Ser Leu Cys His 
                325                 330                 335     


Pro Leu Arg Lys Asn Ala Val Leu Asn Ala Asn Pro Ala Lys Thr Ile 
            340                 345                 350         


Pro Phe Val Leu Ile Ala Ser Asn Phe Ile Gly Val Leu Phe Ser Arg 
        355                 360                 365             


Ser Leu His Tyr Gln Phe Leu Ser Trp Tyr His Trp Thr Leu Pro Ile 
    370                 375                 380                 


Leu Ile Phe Trp Ser Gly Met Pro Phe Phe Val Gly Pro Ile Trp Tyr 
385                 390                 395                 400 


Val Leu His Glu Trp Cys Trp Asn Ser Tyr Pro Pro Asn Ser Gln Ala 
                405                 410                 415     


Ser Thr Leu Leu Leu Ala Leu Asn Thr Val Leu Leu Leu Leu Leu Ala 
            420                 425                 430         


Leu Thr Gln Leu Ser Gly Ser Val Ala Leu Ala Lys Ser His Leu Arg 
        435                 440                 445             


Thr Thr Ser Ser Met Glu Lys Lys Leu Asn 
    450                 455             


<210>  3
<211>  438
<212>  PRT
<213>  Arabidopsis thaliana

<400>  3

Met Ala Gly Ala Ser Ser Pro Ala Ser Leu Arg Ala Ser Arg Ser Arg 
1               5                   10                  15      


Arg Leu Gly Lys Glu Thr Asn Arg Ser Asp Leu Phe Lys Lys Pro Ala 
            20                  25                  30          


Val Pro Phe Ala Phe Ala Leu Ile Leu Ala Asp Ala Ile Leu Val Ala 
        35                  40                  45              


Leu Ile Ile Ala Tyr Val Pro Tyr Thr Lys Ile Asp Trp Asp Ala Tyr 
    50                  55                  60                  


Met Ser Gln Val Ser Gly Phe Leu Gly Gly Glu Arg Asp Tyr Gly Asn 
65                  70                  75                  80  


Leu Lys Gly Asp Thr Gly Pro Leu Val Tyr Pro Ala Gly Phe Leu Tyr 
                85                  90                  95      


Val Tyr Ser Ala Val Gln Asn Leu Thr Gly Gly Glu Val Tyr Pro Ala 
            100                 105                 110         


Gln Ile Leu Phe Gly Val Leu Tyr Ile Val Asn Leu Gly Ile Val Leu 
        115                 120                 125             


Ile Ile Tyr Val Lys Thr Asp Val Val Pro Trp Trp Ala Leu Ser Leu 
    130                 135                 140                 


Leu Cys Leu Ser Lys Arg Ile His Ser Ile Phe Val Leu Arg Leu Phe 
145                 150                 155                 160 


Asn Asp Cys Phe Ala Met Thr Leu Leu His Ala Ser Met Ala Leu Phe 
                165                 170                 175     


Leu Tyr Arg Lys Trp His Leu Gly Met Leu Val Phe Ser Gly Ala Val 
            180                 185                 190         


Ser Val Lys Met Asn Val Leu Leu Tyr Ala Pro Thr Leu Leu Leu Leu 
        195                 200                 205             


Leu Leu Lys Ala Met Asn Ile Ile Gly Val Val Ser Ala Leu Ala Gly 
    210                 215                 220                 


Ala Ala Leu Val Gln Ile Leu Val Gly Leu Pro Phe Leu Ile Thr Tyr 
225                 230                 235                 240 


Pro Val Ser Tyr Ile Ala Asn Ala Phe Asp Leu Gly Arg Val Phe Ile 
                245                 250                 255     


His Phe Trp Ser Val Asn Phe Lys Phe Val Pro Glu Arg Val Phe Val 
            260                 265                 270         


Ser Lys Glu Phe Ala Val Cys Leu Leu Ile Ala His Leu Phe Leu Leu 
        275                 280                 285             


Val Ala Phe Ala Asn Tyr Lys Trp Cys Lys His Glu Gly Gly Ile Ile 
    290                 295                 300                 


Gly Phe Met Arg Ser Arg His Phe Phe Leu Thr Leu Pro Ser Ser Leu 
305                 310                 315                 320 


Ser Phe Ser Asp Val Ser Ala Ser Arg Ile Ile Thr Lys Glu His Val 
                325                 330                 335     


Val Thr Ala Met Phe Val Gly Asn Phe Ile Gly Ile Val Phe Ala Arg 
            340                 345                 350         


Ser Leu His Tyr Gln Phe Tyr Ser Trp Tyr Phe Tyr Ser Leu Pro Tyr 
        355                 360                 365             


Leu Leu Trp Arg Thr Pro Phe Pro Thr Trp Leu Arg Leu Ile Met Phe 
    370                 375                 380                 


Leu Gly Ile Glu Leu Cys Trp Asn Val Tyr Pro Ser Thr Pro Ser Ser 
385                 390                 395                 400 


Ser Gly Leu Leu Leu Cys Leu His Leu Ile Ile Leu Val Gly Leu Trp 
                405                 410                 415     


Leu Ala Pro Ser Val Asp Pro Tyr Gln Leu Lys Glu His Pro Lys Ser 
            420                 425                 430         


Gln Ile His Lys Lys Ala 
        435             


<210>  4
<211>  26
<212>  DNA
<213>  Artificial

<220>
<223>  Alg3 F2.2 primer

<400>  4
cgttcagtga tgtatcagcc tcacgg                                          26


<210>  5
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Alg3-F3 primer

<400>  5
aagcgttgtc catttacgat g                                               21


<210>  6
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  Alg3-R2 primer

<400>  6
ggatttaggg tgttctttga gctgataagg                                      30


<210>  7
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Alg3-R3 primer

<400>  7
cttataattt gcgaacgcca c                                               21


<210>  8
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  actin primer 1

<400>  8
ggtaacattg tgctcagtgg tgg                                             23


<210>  9
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  actin primer 2

<400>  9
aacgacctta atcttcatgc tgc                                             23


<210>  10
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  AtUTR2 primer 1

<400>  10
cacatttatc ggtcaagtct ccgtt                                           25


<210>  11
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  AtUTR2 primer 2

<400>  11
tcgcaggagg cgatggtgat agag                                            24


<210>  12
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  T upstream primer 1

<400>  12
cgcacttatt atcgcctatg ttc                                             23


<210>  13
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  T upstream primer 2

<400>  13
gccctgtatc gcctttcaag                                                 20


<210>  14
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  T downstream primer 1

<400>  14
acttctattc gctaccttat ctac                                            24


<210>  15
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  T downstream primer 2

<400>  15
ggaggacggt gttgatgg                                                   18


<210>  16
<211>  38
<212>  DNA
<213>  Artificial

<220>
<223>  ubiquitin-10 primer 1

<400>  16
gtgacgagct cgtaaatttc tgtgttcctt attctctc                             38


<210>  17
<211>  39
<212>  DNA
<213>  Artificial

<220>
<223>  ubiquitin-10 primer 2

<400>  17
gtgacaagct tctgttaatc agaaaaactc agattaatc                            39


<210>  18
<211>  32
<212>  DNA
<213>  Artificial

<220>
<223>  tobacco alg3 primer 1

<400>  18
gtgaccatgg atgcttatat gtctcaggtt ac                                   32


<210>  19
<211>  33
<212>  DNA
<213>  Artificial

<220>
<223>  tobacco alg3 primer 2

<400>  19
gtgacgagct cagaagtgga tgaaaacacg acc                                  33


<210>  20
<211>  33
<212>  DNA
<213>  Artificial

<220>
<223>  tobacco alg3 primer 3

<400>  20
gtgacggatc catgcttata tgtctcaggt tac                                  33


<210>  21
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  tobacco alg3 primer 4

<400>  21
gtgactctag aagtggatga aaacacgacc                                      30


<210>  22
<211>  304
<212>  DNA
<213>  Arabidopsis thaliana

<400>  22
gtaaatttct gtgttcctta ttctctcaaa atcttcgatt ttgttttcgt tcgatcccaa     60

tttcgtatat gttctttggt ttagattctg ttaatcttag atcgaagacg attttctggg    120

tttgatcgtt agatatcatc ttaattctcg attagggttt catagatatc atccgatttg    180

ttcaaataat ttgagttttg tcgaataatt actcttcgat ttgtgatttc tatctagatc    240

tggtgttagt ttctagtttg tgcgatcgaa tttgtcgatt aatctgagtt tttctgatta    300

acag                                                                 304


<210>  23
<211>  121
<212>  DNA
<213>  Artificial

<220>
<223>  CASesp

<400>  23
ggcgcgcctc gaggcgatcg cagatctatc gatgcatgcc atggtacccg ggagctcgaa     60

ttctagaagc ttctgcagac gcgtcgacgt catatggatc cgcgagagac gtcttaatta    120

a                                                                    121


<210>  24
<211>  1229
<212>  DNA
<213>  Artificial

<220>
<223>  RAP40

<400>  24
ggcgcgccaa gcttgaatta attctactcc aaaaatatca aagatacagt ctcagaagac     60

caaagggcaa ttgagacttt tcaacaaagg gtaatatccg gaaacctcct cggattccat    120

tgcccagcta tctgtcactt tattgtgaag atagtggaaa aggaaggtgg ctcctacaaa    180

tgccatcatt gcgataaagg aaaggccatc gttgaagatg cctctgccga cagtggtccc    240

aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct    300

tcaaagcaag tggattgatg tgataattcc gcatggagtc aaagattcaa atagaggacc    360

taacagaact cgccgtaaag actggcgaac agttcataca gagtctctta cgactcaatg    420

acaagaagaa aatcttcgtc aacatggtgg agcacgacac acttgtctac tccaaaaata    480

tcaaagatac agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat    540

ccggaaacct cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg    600

aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag    660

atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa    720

aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg    780

taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt    840

catttcattt ggagagggtt tttattttta attttctttc aaatacttcc accatgggcg    900

agctcggtac ccggggatcc ccgggtaccg agctcgaatt tccccgatcg ttcaaacatt    960

tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa   1020

tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg   1080

agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa   1140

atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg   1200

gaattccaga tctgcggccg cttaattaa                                     1229


<210>  25
<211>  593
<212>  DNA
<213>  Nicotiana tabacum

<400>  25
ggatgcttat atgtctcagg ttactggctt tcttgaagga gagagggatt atagtaactt     60

gaaaggtgat acagggcctc tagtttatcc agcaggcttt ctttatattt actctgctat    120

tcaatacgtt accggaggtc aagtctatcc tgctcagatt ctttttggct ttctctacat    180

gctggatcta gcaattgtcg tgttcatcta cttgaagact gatgtggtac cttggtgggc    240

tctttccttg ctttctctgt ctaaaagagt tcactctatc tttgttcttc gattatttaa    300

tgattgttta gccactactc tcctccatgc tgcattgatc tcaattattt atcaaaaatg    360

gcatctaggg ttggtaattt tcagtggagc tgtttctata aagatgaatg tgctcctgta    420

tgcaccacct ctgttgctcc tgatggtgaa ggcaatggat attgttggag ttatatctgc    480

tttagcaggt gctgcattag tgcagattgt cattgggctg ccttttgtcc tgtcacatcc    540

agcttcatat atatcaaaag ctttcgatct tggtcgtgtt ttcatccact tct           593


