SEQUENCE LISTING

<110>  CENTOCOR, INC.
 
<120>  ANTI-ALPHA-V IMMUNOLIPOSOME COMPOSITION, METHODS, AND USES

<130>  ALZ5286PCT

<140>  To Be Assigned
<141>  2008-05-12

<150> 60/917,586
<151> 2007-05-11

<160>  5    

<170>  PatentIn version 3.3

<210>  1
<211>  449
<212>  PRT
<213>  Homo sapiens


<220>
<221>  BINDING
<222>  (1)..(119)
<223>  HC Variable Region

<220>
<221>  BINDING
<222>  (31)..(35)
<223>  HC CDR1

<220>
<221>  BINDING
<222>  (50)..(66)
<223>  HC CDR2

<220>
<221>  BINDING
<222>  (99)..(108)
<223>  HC CDR3

<400>  1

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg 
1               5                   10                  15      
Ser Arg Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Arg Tyr 
            20                  25                  30          
Thr Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              
Ala Val Ile Ser Phe Asp Gly Ser Asn Lys Tyr Tyr Val Asp Ser Val 
    50                  55                  60                  

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Glu Asn Thr Leu Tyr 
65                  70                  75                  80  
Leu Gln Val Asn Ile Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      
Ala Arg Glu Ala Arg Gly Ser Tyr Ala Phe Asp Ile Trp Gly Gln Gly 
            100                 105                 110         
Thr Met Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe 
        115                 120                 125             
Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu 
    130                 135                 140                 
Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp 
145                 150                 155                 160 
Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu 
                165                 170                 175     
Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser 
            180                 185                 190         
Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro 
        195                 200                 205             
Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys 
    210                 215                 220                 
Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro 
225                 230                 235                 240 
Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser 
                245                 250                 255     
Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp 
            260                 265                 270         
Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn 
        275                 280                 285             
Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val 
    290                 295                 300                 
Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu 
305                 310                 315                 320 
Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys 
                325                 330                 335     
Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr 
            340                 345                 350         
Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr 
        355                 360                 365             
Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu 
    370                 375                 380                 
Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu 
385                 390                 395                 400 
Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys 
                405                 410                 415     
Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu 
            420                 425                 430         
Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly 
        435                 440                 445             
Lys 
    


<210>  2
<211>  215
<212>  PRT
<213>  Homo sapiens


<220>
<221>  BINDING
<222>  (1)..(108)
<223>  LC Variable Region

<220>
<221>  BINDING
<222>  (24)..(34)
<223>  CDR1

<220>
<221>  BINDING
<222>  (50)..(56)
<223>  CDR2

<220>
<221>  BINDING
<222>  (89)..(97)
<223>  CDR3

<400>  2

Glu Ile Val Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      
Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Val Ser Ser Tyr 
            20                  25                  30          
Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile 
        35                  40                  45              
Tyr Asp Ala Ser Asn Arg Ala Thr Gly Ile Pro Ala Arg Phe Ser Gly 
    50                  55                  60                  
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Glu Pro 
65                  70                  75                  80  
Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Arg Ser Asn Trp Pro Pro 
                85                  90                  95      
Phe Thr Phe Gly Pro Gly Thr Lys Val Asp Ile Lys Arg Thr Val Ala 
            100                 105                 110         
Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser 
        115                 120                 125             
Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu 
    130                 135                 140                 
Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser 
145                 150                 155                 160 
Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu 
                165                 170                 175     
Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val 
            180                 185                 190         
Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys 
        195                 200                 205             
Ser Phe Asn Arg Gly Glu Cys 
    210                 215 


<210>  3
<211>  231
<212>  PRT
<213>  Homo sapiens


<220>
<221>  BINDING
<222>  (1)..(119)
<223>  Heavy chain variable domain

<400>  3

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg 
1               5                   10                  15      
Ser Arg Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Arg Tyr 
            20                  25                  30          
Thr Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              
Ala Val Ile Ser Phe Asp Gly Ser Asn Lys Tyr Tyr Val Asp Ser Val 
    50                  55                  60                  
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Glu Asn Thr Leu Tyr 
65                  70                  75                  80  
Leu Gln Val Asn Ile Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      
Ala Arg Glu Ala Arg Gly Ser Tyr Ala Phe Asp Ile Trp Gly Gln Gly 
            100                 105                 110         
Thr Met Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe 
        115                 120                 125             
Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu 
    130                 135                 140                 
Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp 
145                 150                 155                 160 
Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu 
                165                 170                 175     
Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser 
            180                 185                 190         
Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro 
        195                 200                 205             
Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys 
    210                 215                 220                 
Thr His Thr Cys Pro Gly Lys 
225                 230     


<210>  4
<211>  275
<212>  PRT
<213>  Artificial

<220>
<223>  Construct comprising human immunogobulin regions and artifical 
       sequences


<220>
<221>  SIGNAL
<222>  (1)..(22)
<223>  pelB

<220>
<221>  BINDING
<222>  (23)..(141)
<223>  CNTO95 heavy chain variable region

<220>
<221>  BINDING
<222>  (143)..(156)
<223>  flexible linker sequence of 3 X GGGS

<220>
<221>  BINDING
<222>  (157)..(264)
<223>  CNTO95 light chain variable region

<220>
<221>  BINDING
<222>  (265)..(270)
<223>  Purification sequence

<220>
<221>  BINDING
<222>  (271)..(275)
<223>  Conjugation region

<400>  4

Met Lys Tyr Leu Leu Pro Thr Ala Ala Ala Gly Leu Leu Leu Leu Ala 
1               5                   10                  15      
Ala Gln Pro Ala Met Ala Gln Val Gln Leu Val Glu Ser Gly Gly Gly 
            20                  25                  30          
Val Val Gln Pro Gly Arg Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly 
        35                  40                  45              
Phe Thr Phe Ser Arg Tyr Thr Met His Trp Val Arg Gln Ala Pro Gly 
    50                  55                  60                  
Lys Gly Leu Glu Trp Val Ala Val Ile Ser Phe Asp Gly Ser Asn Lys 
65                  70                  75                  80  
Tyr Tyr Val Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn 
                85                  90                  95      
Ser Glu Asn Thr Leu Tyr Leu Gln Val Asn Ile Leu Arg Ala Glu Asp 
            100                 105                 110         
Thr Ala Val Tyr Tyr Cys Ala Arg Glu Ala Arg Gly Ser Tyr Ala Phe 
        115                 120                 125             
Asp Ile Trp Gly Gln Gly Thr Met Val Thr Val Ser Ser Gly Gly Gly 
    130                 135                 140                 
Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Ile Val Leu 
145                 150                 155                 160 
Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly Glu Arg Ala Thr 
                165                 170                 175     
Leu Ser Cys Arg Ala Ser Gln Ser Val Ser Ser Tyr Leu Ala Trp Tyr 
            180                 185                 190         
Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile Tyr Asp Ala Ser 
        195                 200                 205             
Asn Arg Ala Thr Gly Ile Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly 
    210                 215                 220                 
Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Glu Pro Glu Asp Phe Ala 
225                 230                 235                 240 

Val Tyr Tyr Cys Gln Gln Arg Ser Asn Trp Pro Pro Phe Thr Phe Gly 
                245                 250                 255     
Pro Gly Thr Lys Val Asp Ile Lys His His His His His His Gly Gly 
            260                 265                 270         
Gly Gly Cys 
        275 


<210>  5
<211>  893
<212>  DNA
<213>  Artificial

<220>
<223>  human codons replaced with E. coli preferred codons


<220>
<221>  sig_peptide
<222>  (57)..(122)
<223>  pelB signal

<220>
<221>  terminator
<222>  (882)..(887)
<223>  Tandem stop codons

<400>  5
gaattctatg gaacgataaa tgcccatgaa aattctattt caaggagaca gtcataatga    60
aatacctgct gccgaccgct gctgctggtc tgctgctgct ggcggcccag ccggccatgg   120
ctcaggtgca gctggtggag tctggtggtg gcgtggtcca gcctggtcgt tcccgtcgcc   180
tctcctgtgc agcctctggt ttcaccttca gtcgttatac tatgcactgg gtccgccagg   240
ctccaggcaa gggtctggag tgggtggcag ttatctcatt tgatggtagc aacaaatact   300
acgtagactc cgtgaagggc cgtttcacca tctcccgtga caactccgag aacacgctgt   360
atctgcaagt gaacatcctg cgtgctgagg acacggctgt gtattactgt gcgcgtgagg   420
cccgtggttc ctatgctttt gatatctggg gccaaggtac catggtcacc gtctcttccg   480
gtggcggtgg ctccggtggc ggtggctccg gtggcggtgg atccgaaatt gtgctgaccc   540
agtctccagc caccctgtct ctgtctccag gtgaacgtgc caccctctcc tgccgtgcca   600
gtcagagtgt tagcagctac ctggcctggt accaacagaa acctggccag gctccgcgtc   660
tcctcatcta tgatgcatcc aaccgtgcca ctggcatccc agcccgtttc agtggcagtg   720
gttctggtac cgacttcact ctcaccatca gcagcctgga gcctgaagat tttgcagttt   780
attactgtca gcagcgtagc aactggcctc cattcacttt cggccctggt accaaagtgg   840
atatcaaaca ccaccatcac caccatggtg gtggtggttg ctaataactc gag          893



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