                         SEQUENCE LISTING

<110>  E.I. duPont de Nemours and Company, Inc.
       Damude, Howard
       Zhu, Quinn
 
<120>  Delta-5 Desaturases and Their Use in Making Polyunsaturated Fatty
        Acids

<130>  CL3922

<150>  US 60/915733
<151>  2007-05-03

<160>  44    

<170>  PatentIn version 3.4

<210>  1
<211>  1350
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(1350)
<223>  delta-5 desaturase

<300>
<302>  DELTA-5 DESATURASE AND ITS USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  WO2007/136671
<311>  2007-05-16
<312>  2007-11-29
<313>  (1)..(1350)

<300>
<302>  DELTA-5 DESATURASE AND ITS USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  US-2007-0292924-A1
<311>  2007-05-15
<312>  2007-12-20
<313>  (1)..(1350)

<400>  1
atg gct ctc agt ctt acc aca gaa cag ctg tta gaa cgc cct gat ttg       48
Met Ala Leu Ser Leu Thr Thr Glu Gln Leu Leu Glu Arg Pro Asp Leu         
1               5                   10                  15              

gtt gcg att gat ggc atc ctc tac gac ctt gaa ggg ctt gcc aaa gtt       96
Val Ala Ile Asp Gly Ile Leu Tyr Asp Leu Glu Gly Leu Ala Lys Val         
            20                  25                  30                  

cat cca gga gga gat ttg att ctc gct tct ggt gcc tct gat gcc tcc      144
His Pro Gly Gly Asp Leu Ile Leu Ala Ser Gly Ala Ser Asp Ala Ser         
        35                  40                  45                      

cct ctc ttt tat tca atg cat cca tac gtc aaa ccg gag aat tcc aaa      192
Pro Leu Phe Tyr Ser Met His Pro Tyr Val Lys Pro Glu Asn Ser Lys         
    50                  55                  60                          

ttg ctt caa cag ttc gtc cga ggg aag cat gac cgc acc tcg aag gac      240
Leu Leu Gln Gln Phe Val Arg Gly Lys His Asp Arg Thr Ser Lys Asp         
65                  70                  75                  80          

att gtc tac acg tat gat tct ccc ttc gca caa gac gtt aag cgg aca      288
Ile Val Tyr Thr Tyr Asp Ser Pro Phe Ala Gln Asp Val Lys Arg Thr         
                85                  90                  95              

atg cgc gag gtg atg aaa ggg agg aac tgg tac gca acc cct ggc ttc      336
Met Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly Phe         
            100                 105                 110                 

tgg ctg cgc acc gtt ggg atc atc gcc gtg acg gcc ttt tgc gag tgg      384
Trp Leu Arg Thr Val Gly Ile Ile Ala Val Thr Ala Phe Cys Glu Trp         
        115                 120                 125                     

cac tgg gct acc acg ggg atg gtg ctg tgg ggc ctg ttg act gga ttc      432
His Trp Ala Thr Thr Gly Met Val Leu Trp Gly Leu Leu Thr Gly Phe         
    130                 135                 140                         

atg cac atg cag atc ggc tta tcc atc cag cat gat gcg tcc cac ggg      480
Met His Met Gln Ile Gly Leu Ser Ile Gln His Asp Ala Ser His Gly         
145                 150                 155                 160         

gcc atc agc aag aag cct tgg gtc aac gcc ctc ttc gcc tac ggc att      528
Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Leu Phe Ala Tyr Gly Ile         
                165                 170                 175             

gac gtc atc gga tcg tcc cgg tgg att tgg ctg cag tcg cac atc atg      576
Asp Val Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile Met         
            180                 185                 190                 

cgg cac cac acc tac acc aac cag cac ggc ctc gac ctg gat gcg gag      624
Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala Glu         
        195                 200                 205                     

tcg gca gag ccg ttc ctg gtg ttc cac aac tac ccc gcc gca aac acc      672
Ser Ala Glu Pro Phe Leu Val Phe His Asn Tyr Pro Ala Ala Asn Thr         
    210                 215                 220                         

gcc cga aag tgg ttc cac cgc ttc caa gct tgg tac atg tac ctt gtg      720
Ala Arg Lys Trp Phe His Arg Phe Gln Ala Trp Tyr Met Tyr Leu Val         
225                 230                 235                 240         

ctg ggg gca tac ggg gta tcg ctg gtg tac aac ccg ctc tac att ttc      768
Leu Gly Ala Tyr Gly Val Ser Leu Val Tyr Asn Pro Leu Tyr Ile Phe         
                245                 250                 255             

cgg atg cag cac aat gac acc atc cca gag tct gtc acg gcc atg cgg      816
Arg Met Gln His Asn Asp Thr Ile Pro Glu Ser Val Thr Ala Met Arg         
            260                 265                 270                 

gag aat ggc ttt ctg cgg cgc tac cgc aca ctt gca ttc gtg atg cga      864
Glu Asn Gly Phe Leu Arg Arg Tyr Arg Thr Leu Ala Phe Val Met Arg         
        275                 280                 285                     

gct ttc ttc atc ttc cgg acc gca ttc ttg ccc tgg tac ctc act ggg      912
Ala Phe Phe Ile Phe Arg Thr Ala Phe Leu Pro Trp Tyr Leu Thr Gly         
    290                 295                 300                         

acc tca ttg ctg atc acc att cct ctg gtg ccc act gca act ggt gcc      960
Thr Ser Leu Leu Ile Thr Ile Pro Leu Val Pro Thr Ala Thr Gly Ala         
305                 310                 315                 320         

ttc ttg acg ttc ttc ttc att ttg tcc cac aat ttt gat ggc tcc gaa     1008
Phe Leu Thr Phe Phe Phe Ile Leu Ser His Asn Phe Asp Gly Ser Glu         
                325                 330                 335             

cgg atc ccc gac aag aac tgc aag gtt aag agc tct gag aag gac gtt     1056
Arg Ile Pro Asp Lys Asn Cys Lys Val Lys Ser Ser Glu Lys Asp Val         
            340                 345                 350                 

gag gct gac caa att gac tgg tat cgg gcg cag gtg gag acg tcc tcc     1104
Glu Ala Asp Gln Ile Asp Trp Tyr Arg Ala Gln Val Glu Thr Ser Ser         
        355                 360                 365                     

aca tac ggt ggc ccc atc gcc atg ttc ttc act ggc ggt ctc aat ttc     1152
Thr Tyr Gly Gly Pro Ile Ala Met Phe Phe Thr Gly Gly Leu Asn Phe         
    370                 375                 380                         

cag atc gag cac cac ctc ttt ccc cgg atg tcg tct tgg cac tac ccc     1200
Gln Ile Glu His His Leu Phe Pro Arg Met Ser Ser Trp His Tyr Pro         
385                 390                 395                 400         

ttc gtc cag cag gcg gtc cgg gag tgt tgc gaa cgc cat gga gtg cga     1248
Phe Val Gln Gln Ala Val Arg Glu Cys Cys Glu Arg His Gly Val Arg         
                405                 410                 415             

tat gtt ttc tac cct acc atc gtc ggc aac atc atc tcc acc ctg aag     1296
Tyr Val Phe Tyr Pro Thr Ile Val Gly Asn Ile Ile Ser Thr Leu Lys         
            420                 425                 430                 

tac atg cat aag gtg ggt gtc gtc cac tgc gtg aag gac gca cag gat     1344
Tyr Met His Lys Val Gly Val Val His Cys Val Lys Asp Ala Gln Asp         
        435                 440                 445                     

tcc tga                                                             1350
Ser                                                                     
                                                                        


<210>  2
<211>  449
<212>  PRT
<213>  Euglena gracilis

<400>  2

Met Ala Leu Ser Leu Thr Thr Glu Gln Leu Leu Glu Arg Pro Asp Leu 
1               5                   10                  15      


Val Ala Ile Asp Gly Ile Leu Tyr Asp Leu Glu Gly Leu Ala Lys Val 
            20                  25                  30          


His Pro Gly Gly Asp Leu Ile Leu Ala Ser Gly Ala Ser Asp Ala Ser 
        35                  40                  45              


Pro Leu Phe Tyr Ser Met His Pro Tyr Val Lys Pro Glu Asn Ser Lys 
    50                  55                  60                  


Leu Leu Gln Gln Phe Val Arg Gly Lys His Asp Arg Thr Ser Lys Asp 
65                  70                  75                  80  


Ile Val Tyr Thr Tyr Asp Ser Pro Phe Ala Gln Asp Val Lys Arg Thr 
                85                  90                  95      


Met Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly Phe 
            100                 105                 110         


Trp Leu Arg Thr Val Gly Ile Ile Ala Val Thr Ala Phe Cys Glu Trp 
        115                 120                 125             


His Trp Ala Thr Thr Gly Met Val Leu Trp Gly Leu Leu Thr Gly Phe 
    130                 135                 140                 


Met His Met Gln Ile Gly Leu Ser Ile Gln His Asp Ala Ser His Gly 
145                 150                 155                 160 


Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Leu Phe Ala Tyr Gly Ile 
                165                 170                 175     


Asp Val Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile Met 
            180                 185                 190         


Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala Glu 
        195                 200                 205             


Ser Ala Glu Pro Phe Leu Val Phe His Asn Tyr Pro Ala Ala Asn Thr 
    210                 215                 220                 


Ala Arg Lys Trp Phe His Arg Phe Gln Ala Trp Tyr Met Tyr Leu Val 
225                 230                 235                 240 


Leu Gly Ala Tyr Gly Val Ser Leu Val Tyr Asn Pro Leu Tyr Ile Phe 
                245                 250                 255     


Arg Met Gln His Asn Asp Thr Ile Pro Glu Ser Val Thr Ala Met Arg 
            260                 265                 270         


Glu Asn Gly Phe Leu Arg Arg Tyr Arg Thr Leu Ala Phe Val Met Arg 
        275                 280                 285             


Ala Phe Phe Ile Phe Arg Thr Ala Phe Leu Pro Trp Tyr Leu Thr Gly 
    290                 295                 300                 


Thr Ser Leu Leu Ile Thr Ile Pro Leu Val Pro Thr Ala Thr Gly Ala 
305                 310                 315                 320 


Phe Leu Thr Phe Phe Phe Ile Leu Ser His Asn Phe Asp Gly Ser Glu 
                325                 330                 335     


Arg Ile Pro Asp Lys Asn Cys Lys Val Lys Ser Ser Glu Lys Asp Val 
            340                 345                 350         


Glu Ala Asp Gln Ile Asp Trp Tyr Arg Ala Gln Val Glu Thr Ser Ser 
        355                 360                 365             


Thr Tyr Gly Gly Pro Ile Ala Met Phe Phe Thr Gly Gly Leu Asn Phe 
    370                 375                 380                 


Gln Ile Glu His His Leu Phe Pro Arg Met Ser Ser Trp His Tyr Pro 
385                 390                 395                 400 


Phe Val Gln Gln Ala Val Arg Glu Cys Cys Glu Arg His Gly Val Arg 
                405                 410                 415     


Tyr Val Phe Tyr Pro Thr Ile Val Gly Asn Ile Ile Ser Thr Leu Lys 
            420                 425                 430         


Tyr Met His Lys Val Gly Val Val His Cys Val Lys Asp Ala Gln Asp 
        435                 440                 445             


Ser 
    


<210>  3
<211>  1524
<212>  DNA
<213>  Euglena anabaena

<400>  3
acccagtgta cacctttgac agtcccttcg cccaggatgt caagcagagc gttcgggagg     60

tcatgaaggg gcgcaactgg tacgccacgc ccggcttttg gctgcggacc gcgctgatca    120

tcgcgtgcac tgccataggc gaatggtatt ggatcactac cggggcagtg atgtggggca    180

tcttcaccgg gtacttccac agccagattg ggttggcgat tcaacacgat gcctctcacg    240

gagccatcag caaaaagccc tgggtgaacg cctttttcgc ctacggcatc gacgccattg    300

gatcctcccg ctggatctgg ctgcagtccc acattatgcg ccaccacacc tacaccaacc    360

agcatggcct ggacctggac gctgcctcgg cggagccgtt cattttgttc cactcctacc    420

cggcaacaaa tgcgtcacga aagtggtacc atcggttcca ggcgtggtac atgtacatcg    480

ttttggggat gtatggtgtg tcgatggtgt acaatccgat gtacttgttc acgatgcagc    540

acaacgacac aatcccagag gccacctctc ttagaccagg cagctttttc aaccggcagc    600

gcgccttcgc cgtttccctc cgcctactgt tcatcttccg caacgccttc ctcccctggt    660

acatcgcggg cgcctctccg ctgctcacca tcctgctggt gccaacggtc acaggcatct    720

tcttgacatt tgtttttgtg ctgtcccata actttgaagg cgctgagcgg acccccgaaa    780

agaactgcaa ggccaaaagg gccaaggagg ggaaggaggt ccgcgatgta gaggaggacc    840

gggtggactg gtaccgggcg caggccgaga ccgcggcgac ctacgggggc agcgtcggga    900

tgatgctgac cggcggtttg aacctgcaga tcgagcacca cttgttcccc cgcatgtcct    960

cttggcacta ccccttcatc caagatacgg tgcgggaatg ttgcaagcgc catggcgtgc   1020

gctacacata ctacccgacc atcctggaga atataatgtc cacgctccgc tacatgcaga   1080

aggtgggcgt ggcccacaca attcaggatg cccaggaatt ctgagtgagt tcgatccgca   1140

tcgacgtcta ccatttttga tgctgtctat tcctgttttc agtcacctcc agcattctca   1200

tggctggtga ccactgcccc tctaacccat tgtgacacac cgccaaagac tttgcctctt   1260

ttttttccct ttcttttgtc ctcggggtgc tttggccggt gtttactcgc cttgcagtcc   1320

ccgcaaacga ccgacgttta agctccgttg ttgactgggc cgctcgtaaa cccatctgca   1380

ggttgaggct cccatggaga attgtgatgg ctgattagga ggtggcgggg catacatgcc   1440

tcgacactca aagccgggcg gcttctggat tcgaaaacgc aaatgggcgc tttggaaaaa   1500

aaaaaaaaaa aaaaaaaaaa aaaa                                          1524


<210>  4
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide YL794

<400>  4
tttccatggc tctcagtctt accacagaac ag                                   32


<210>  5
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide YL797

<400>  5
gtacatgtac caagcttgga agcggtg                                         27


<210>  6
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide YL796

<400>  6
caccgcttcc aagcttggta catgtac                                         27


<210>  7
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide YL795

<400>  7
tttgcggccg cttaggaatc ctgtgcgtcc ttcacgcag                            39


<210>  8
<211>  8165
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  plasmid pZUF17

<400>  8
gtacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca     60

ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat    120

taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc    180

tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca    240

aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca    300

aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg    360

ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg    420

acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt    480

ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt    540

tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc    600

tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt    660

gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt    720

agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc    780

tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa    840

agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt    900

tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct    960

acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta   1020

tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa   1080

agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc   1140

tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact   1200

acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc   1260

tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt   1320

ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta   1380

agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg   1440

tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt   1500

acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc   1560

agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt   1620

actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc   1680

tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc   1740

gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa   1800

ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac   1860

tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa   1920

aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt   1980

tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa   2040

tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct   2100

gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc   2160

gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc   2220

acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt   2280

agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg   2340

ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt   2400

ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta   2460

taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt   2520

aacgcgaatt ttaacaaaat attaacgctt acaatttcca ttcgccattc aggctgcgca   2580

actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg   2640

gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta   2700

aaacgacggc cagtgaattg taatacgact cactataggg cgaattgggt accgggcccc   2760

ccctcgaggt cgatggtgtc gataagcttg atatcgaatt catgtcacac aaaccgatct   2820

tcgcctcaag gaaacctaat tctacatccg agagactgcc gagatccagt ctacactgat   2880

taattttcgg gccaataatt taaaaaaatc gtgttatata atattatatg tattatatat   2940

atacatcatg atgatactga cagtcatgtc ccattgctaa atagacagac tccatctgcc   3000

gcctccaact gatgttctca atatttaagg ggtcatctcg cattgtttaa taataaacag   3060

actccatcta ccgcctccaa atgatgttct caaaatatat tgtatgaact tatttttatt   3120

acttagtatt attagacaac ttacttgctt tatgaaaaac acttcctatt taggaaacaa   3180

tttataatgg cagttcgttc atttaacaat ttatgtagaa taaatgttat aaatgcgtat   3240

gggaaatctt aaatatggat agcataaatg atatctgcat tgcctaattc gaaatcaaca   3300

gcaacgaaaa aaatcccttg tacaacataa atagtcatcg agaaatatca actatcaaag   3360

aacagctatt cacacgttac tattgagatt attattggac gagaatcaca cactcaactg   3420

tctttctctc ttctagaaat acaggtacaa gtatgtacta ttctcattgt tcatacttct   3480

agtcatttca tcccacatat tccttggatt tctctccaat gaatgacatt ctatcttgca   3540

aattcaacaa ttataataag atataccaaa gtagcggtat agtggcaatc aaaaagcttc   3600

tctggtgtgc ttctcgtatt tatttttatt ctaatgatcc attaaaggta tatatttatt   3660

tcttgttata taatcctttt gtttattaca tgggctggat acataaaggt attttgattt   3720

aattttttgc ttaaattcaa tcccccctcg ttcagtgtca actgtaatgg taggaaatta   3780

ccatactttt gaagaagcaa aaaaaatgaa agaaaaaaaa aatcgtattt ccaggttaga   3840

cgttccgcag aatctagaat gcggtatgcg gtacattgtt cttcgaacgt aaaagttgcg   3900

ctccctgaga tattgtacat ttttgctttt acaagtacaa gtacatcgta caactatgta   3960

ctactgttga tgcatccaca acagtttgtt ttgttttttt ttgttttttt tttttctaat   4020

gattcattac cgctatgtat acctacttgt acttgtagta agccgggtta ttggcgttca   4080

attaatcata gacttatgaa tctgcacggt gtgcgctgcg agttactttt agcttatgca   4140

tgctacttgg gtgtaatatt gggatctgtt cggaaatcaa cggatgctca atcgatttcg   4200

acagtaatta attaagtcat acacaagtca gctttcttcg agcctcatat aagtataagt   4260

agttcaacgt attagcactg tacccagcat ctccgtatcg agaaacacaa caacatgccc   4320

cattggacag atcatgcgga tacacaggtt gtgcagtatc atacatactc gatcagacag   4380

gtcgtctgac catcatacaa gctgaacaag cgctccatac ttgcacgctc tctatataca   4440

cagttaaatt acatatccat agtctaacct ctaacagtta atcttctggt aagcctccca   4500

gccagccttc tggtatcgct tggcctcctc aataggatct cggttctggc cgtacagacc   4560

tcggccgaca attatgatat ccgttccggt agacatgaca tcctcaacag ttcggtactg   4620

ctgtccgaga gcgtctccct tgtcgtcaag acccaccccg ggggtcagaa taagccagtc   4680

ctcagagtcg cccttaggtc ggttctgggc aatgaagcca accacaaact cggggtcgga   4740

tcgggcaagc tcaatggtct gcttggagta ctcgccagtg gccagagagc ccttgcaaga   4800

cagctcggcc agcatgagca gacctctggc cagcttctcg ttgggagagg ggactaggaa   4860

ctccttgtac tgggagttct cgtagtcaga gacgtcctcc ttcttctgtt cagagacagt   4920

ttcctcggca ccagctcgca ggccagcaat gattccggtt ccgggtacac cgtgggcgtt   4980

ggtgatatcg gaccactcgg cgattcggtg acaccggtac tggtgcttga cagtgttgcc   5040

aatatctgcg aactttctgt cctcgaacag gaagaaaccg tgcttaagag caagttcctt   5100

gagggggagc acagtgccgg cgtaggtgaa gtcgtcaatg atgtcgatat gggttttgat   5160

catgcacaca taaggtccga ccttatcggc aagctcaatg agctccttgg tggtggtaac   5220

atccagagaa gcacacaggt tggttttctt ggctgccacg agcttgagca ctcgagcggc   5280

aaaggcggac ttgtggacgt tagctcgagc ttcgtaggag ggcattttgg tggtgaagag   5340

gagactgaaa taaatttagt ctgcagaact ttttatcgga accttatctg gggcagtgaa   5400

gtatatgtta tggtaatagt tacgagttag ttgaacttat agatagactg gactatacgg   5460

ctatcggtcc aaattagaaa gaacgtcaat ggctctctgg gcgtcgcctt tgccgacaaa   5520

aatgtgatca tgatgaaagc cagcaatgac gttgcagctg atattgttgt cggccaaccg   5580

cgccgaaaac gcagctgtca gacccacagc ctccaacgaa gaatgtatcg tcaaagtgat   5640

ccaagcacac tcatagttgg agtcgtactc caaaggcggc aatgacgagt cagacagata   5700

ctcgtcgact caggcgacga cggaattcct gcagcccatc tgcagaattc aggagagacc   5760

gggttggcgg cgtatttgtg tcccaaaaaa cagccccaat tgccccggag aagacggcca   5820

ggccgcctag atgacaaatt caacaactca cagctgactt tctgccattg ccactagggg   5880

ggggcctttt tatatggcca agccaagctc tccacgtcgg ttgggctgca cccaacaata   5940

aatgggtagg gttgcaccaa caaagggatg ggatgggggg tagaagatac gaggataacg   6000

gggctcaatg gcacaaataa gaacgaatac tgccattaag actcgtgatc cagcgactga   6060

caccattgca tcatctaagg gcctcaaaac tacctcggaa ctgctgcgct gatctggaca   6120

ccacagaggt tccgagcact ttaggttgca ccaaatgtcc caccaggtgc aggcagaaaa   6180

cgctggaaca gcgtgtacag tttgtcttaa caaaaagtga gggcgctgag gtcgagcagg   6240

gtggtgtgac ttgttatagc ctttagagct gcgaaagcgc gtatggattt ggctcatcag   6300

gccagattga gggtctgtgg acacatgtca tgttagtgta cttcaatcgc cccctggata   6360

tagccccgac aataggccgt ggcctcattt ttttgccttc cgcacatttc cattgctcgg   6420

tacccacacc ttgcttctcc tgcacttgcc aaccttaata ctggtttaca ttgaccaaca   6480

tcttacaagc ggggggcttg tctagggtat atataaacag tggctctccc aatcggttgc   6540

cagtctcttt tttcctttct ttccccacag attcgaaatc taaactacac atcacacaat   6600

gcctgttact gacgtcctta agcgaaagtc cggtgtcatc gtcggcgacg atgtccgagc   6660

cgtgagtatc cacgacaaga tcagtgtcga gacgacgcgt tttgtgtaat gacacaatcc   6720

gaaagtcgct agcaacacac actctctaca caaactaacc cagctctcca tggctgagga   6780

taagaccaag gtcgagttcc ctaccctgac tgagctgaag cactctatcc ctaacgcttg   6840

ctttgagtcc aacctcggac tctcgctcta ctacactgcc cgagcgatct tcaacgcatc   6900

tgcctctgct gctctgctct acgctgcccg atctactccc ttcattgccg ataacgttct   6960

gctccacgct ctggtttgcg ccacctacat ctacgtgcag ggtgtcatct tctggggttt   7020

ctttaccgtc ggtcacgact gtggtcactc tgccttctcc cgataccact ccgtcaactt   7080

catcattggc tgcatcatgc actctgccat tctgactccc ttcgagtcct ggcgagtgac   7140

ccaccgacac catcacaaga acactggcaa cattgataag gacgagatct tctaccctca   7200

tcggtccgtc aaggacctcc aggacgtgcg acaatgggtc tacaccctcg gaggtgcttg   7260

gtttgtctac ctgaaggtcg gatatgctcc tcgaaccatg tcccactttg acccctggga   7320

ccctctcctg cttcgacgag cctccgctgt catcgtgtcc ctcggagtct gggctgcctt   7380

cttcgctgcc tacgcctacc tcacatactc gctcggcttt gccgtcatgg gcctctacta   7440

ctatgctcct ctctttgtct ttgcttcgtt cctcgtcatt actaccttct tgcatcacaa   7500

cgacgaagct actccctggt acggtgactc ggagtggacc tacgtcaagg gcaacctgag   7560

ctccgtcgac cgatcgtacg gagctttcgt ggacaacctg tctcaccaca ttggcaccca   7620

ccaggtccat cacttgttcc ctatcattcc ccactacaag ctcaacgaag ccaccaagca   7680

ctttgctgcc gcttaccctc acctcgtgag acgtaacgac gagcccatca ttactgcctt   7740

cttcaagacc gctcacctct ttgtcaacta cggagctgtg cccgagactg ctcagatttt   7800

caccctcaaa gagtctgccg ctgcagccaa ggccaagagc gactaagcgg ccgcaagtgt   7860

ggatggggaa gtgagtgccc ggttctgtgt gcacaattgg caatccaaga tggatggatt   7920

caacacaggg atatagcgag ctacgtggtg gtgcgaggat atagcaacgg atatttatgt   7980

ttgacacttg agaatgtacg atacaagcac tgtccaagta caatactaaa catactgtac   8040

atactcatac tcgtacccgg gcaacggttt cacttgagtg cagtggctag tgctcttact   8100

cgtacagtgt gcaatactgc gtatcatagt ctttgatgta tatcgtattc attcatgtta   8160

gttgc                                                               8165


<210>  9
<211>  8438
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  plasmid pDMW367

<400>  9
catggctctc agtcttacca cagaacagct gttagaacgc cctgatttgg ttgcgattga     60

tggcatcctc tacgaccttg aagggcttgc caaagttcat ccaggaggag atttgattct    120

cgcttctggt gcctctgatg cctcccctct cttttattca atgcatccat acgtcaaacc    180

ggagaattcc aaattgcttc aacagttcgt ccgagggaag catgaccgca cctcgaagga    240

cattgtctac acgtatgatt ctcccttcgc acaagacgtt aagcggacaa tgcgcgaggt    300

gatgaaaggg aggaactggt acgcaacccc tggcttctgg ctgcgcaccg ttgggatcat    360

cgccgtgacg gccttttgcg agtggcactg ggctaccacg gggatggtgc tgtggggcct    420

gttgactgga ttcatgcaca tgcagatcgg cttatccatc cagcatgatg cgtcccacgg    480

ggccatcagc aagaagcctt gggtcaacgc cctcttcgcc tacggcattg acgtcatcgg    540

atcgtcccgg tggatttggc tgcagtcgca catcatgcgg caccacacct acaccaacca    600

gcacggcctc gacctggatg cggagtcggc agagccgttc ctggtgttcc acaactaccc    660

cgccgcaaac accgcccgaa agtggttcca ccgcttccaa gcttggtaca tgtaccttgt    720

gctgggggca tacggggtat cgctggtgta caacccgctc tacattttcc ggatgcagca    780

caatgacacc atcccagagt ctgtcacggc catgcgggaa aatggctttc tgcggcgcta    840

ccgcacactt gcattcgtga tgcgagcttt cttcatcttc cggaccgcat tcttgccctg    900

gtacctcact gggacctcat tgctgatcac cattcctctg gtgcccaccg caactggtgc    960

cttcttgacg ttcttcttca ttttgtccca caattttgat ggctccgaac ggatccccga   1020

caagaactgc aaggttaaga gatctgagaa ggacgttgag gctgaccaaa ttgactggta   1080

tcgggcgcag gtggagacgt cctccacata cggtggcccc atcgccatgt tcttcactgg   1140

cggtctcaat ttccagatcg agcaccacct ctttccccgg atgtcgtctt ggcactaccc   1200

cttcgtccag caggcggtcc gggagtgttg cgaacgccat ggagtgcgat atgttttcta   1260

ccctaccatc gtcggcaaca tcatctccac cctgaagtac atgcataagg tgggtgtcgt   1320

ccactgcgtg aaggacgcac aggattccta agcggccgca agtgtggatg gggaagtgag   1380

tgcccggttc tgtgtgcaca attggcaatc caagatggat ggattcaaca cagggatata   1440

gcgagctacg tggtggtgcg aggatatagc aacggatatt tatgtttgac acttgagaat   1500

gtacgataca agcactgtcc aagtacaata ctaaacatac tgtacatact catactcgta   1560

cccgggcaac ggtttcactt gagtgcagtg gctagtgctc ttactcgtac agtgtgcaat   1620

actgcgtatc atagtctttg atgtatatcg tattcattca tgttagttgc gtacgagccg   1680

gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt   1740

tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg   1800

gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg   1860

actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa   1920

tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc   1980

aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc   2040

ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat   2100

aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc   2160

cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct   2220

cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg   2280

aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc   2340

cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga   2400

ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa   2460

ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta   2520

gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc   2580

agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg   2640

acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga   2700

tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg   2760

agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct   2820

gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg   2880

agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc   2940

cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa   3000

ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc   3060

cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt   3120

cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc   3180

ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt   3240

tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc   3300

catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt   3360

gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata   3420

gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga   3480

tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag   3540

catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa   3600

aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt   3660

attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga   3720

aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgcgccct   3780

gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg   3840

ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg   3900

gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac   3960

ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct   4020

gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt   4080

tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt   4140

tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt   4200

ttaacaaaat attaacgctt acaatttcca ttcgccattc aggctgcgca actgttggga   4260

agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc   4320

aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc   4380

cagtgaattg taatacgact cactataggg cgaattgggt accgggcccc ccctcgaggt   4440

cgatggtgtc gataagcttg atatcgaatt catgtcacac aaaccgatct tcgcctcaag   4500

gaaacctaat tctacatccg agagactgcc gagatccagt ctacactgat taattttcgg   4560

gccaataatt taaaaaaatc gtgttatata atattatatg tattatatat atacatcatg   4620

atgatactga cagtcatgtc ccattgctaa atagacagac tccatctgcc gcctccaact   4680

gatgttctca atatttaagg ggtcatctcg cattgtttaa taataaacag actccatcta   4740

ccgcctccaa atgatgttct caaaatatat tgtatgaact tatttttatt acttagtatt   4800

attagacaac ttacttgctt tatgaaaaac acttcctatt taggaaacaa tttataatgg   4860

cagttcgttc atttaacaat ttatgtagaa taaatgttat aaatgcgtat gggaaatctt   4920

aaatatggat agcataaatg atatctgcat tgcctaattc gaaatcaaca gcaacgaaaa   4980

aaatcccttg tacaacataa atagtcatcg agaaatatca actatcaaag aacagctatt   5040

cacacgttac tattgagatt attattggac gagaatcaca cactcaactg tctttctctc   5100

ttctagaaat acaggtacaa gtatgtacta ttctcattgt tcatacttct agtcatttca   5160

tcccacatat tccttggatt tctctccaat gaatgacatt ctatcttgca aattcaacaa   5220

ttataataag atataccaaa gtagcggtat agtggcaatc aaaaagcttc tctggtgtgc   5280

ttctcgtatt tatttttatt ctaatgatcc attaaaggta tatatttatt tcttgttata   5340

taatcctttt gtttattaca tgggctggat acataaaggt attttgattt aattttttgc   5400

ttaaattcaa tcccccctcg ttcagtgtca actgtaatgg taggaaatta ccatactttt   5460

gaagaagcaa aaaaaatgaa agaaaaaaaa aatcgtattt ccaggttaga cgttccgcag   5520

aatctagaat gcggtatgcg gtacattgtt cttcgaacgt aaaagttgcg ctccctgaga   5580

tattgtacat ttttgctttt acaagtacaa gtacatcgta caactatgta ctactgttga   5640

tgcatccaca acagtttgtt ttgttttttt ttgttttttt tttttctaat gattcattac   5700

cgctatgtat acctacttgt acttgtagta agccgggtta ttggcgttca attaatcata   5760

gacttatgaa tctgcacggt gtgcgctgcg agttactttt agcttatgca tgctacttgg   5820

gtgtaatatt gggatctgtt cggaaatcaa cggatgctca atcgatttcg acagtaatta   5880

attaagtcat acacaagtca gctttcttcg agcctcatat aagtataagt agttcaacgt   5940

attagcactg tacccagcat ctccgtatcg agaaacacaa caacatgccc cattggacag   6000

atcatgcgga tacacaggtt gtgcagtatc atacatactc gatcagacag gtcgtctgac   6060

catcatacaa gctgaacaag cgctccatac ttgcacgctc tctatataca cagttaaatt   6120

acatatccat agtctaacct ctaacagtta atcttctggt aagcctccca gccagccttc   6180

tggtatcgct tggcctcctc aataggatct cggttctggc cgtacagacc tcggccgaca   6240

attatgatat ccgttccggt agacatgaca tcctcaacag ttcggtactg ctgtccgaga   6300

gcgtctccct tgtcgtcaag acccaccccg ggggtcagaa taagccagtc ctcagagtcg   6360

cccttaggtc ggttctgggc aatgaagcca accacaaact cggggtcgga tcgggcaagc   6420

tcaatggtct gcttggagta ctcgccagtg gccagagagc ccttgcaaga cagctcggcc   6480

agcatgagca gacctctggc cagcttctcg ttgggagagg ggactaggaa ctccttgtac   6540

tgggagttct cgtagtcaga gacgtcctcc ttcttctgtt cagagacagt ttcctcggca   6600

ccagctcgca ggccagcaat gattccggtt ccgggtacac cgtgggcgtt ggtgatatcg   6660

gaccactcgg cgattcggtg acaccggtac tggtgcttga cagtgttgcc aatatctgcg   6720

aactttctgt cctcgaacag gaagaaaccg tgcttaagag caagttcctt gagggggagc   6780

acagtgccgg cgtaggtgaa gtcgtcaatg atgtcgatat gggttttgat catgcacaca   6840

taaggtccga ccttatcggc aagctcaatg agctccttgg tggtggtaac atccagagaa   6900

gcacacaggt tggttttctt ggctgccacg agcttgagca ctcgagcggc aaaggcggac   6960

ttgtggacgt tagctcgagc ttcgtaggag ggcattttgg tggtgaagag gagactgaaa   7020

taaatttagt ctgcagaact ttttatcgga accttatctg gggcagtgaa gtatatgtta   7080

tggtaatagt tacgagttag ttgaacttat agatagactg gactatacgg ctatcggtcc   7140

aaattagaaa gaacgtcaat ggctctctgg gcgtcgcctt tgccgacaaa aatgtgatca   7200

tgatgaaagc cagcaatgac gttgcagctg atattgttgt cggccaaccg cgccgaaaac   7260

gcagctgtca gacccacagc ctccaacgaa gaatgtatcg tcaaagtgat ccaagcacac   7320

tcatagttgg agtcgtactc caaaggcggc aatgacgagt cagacagata ctcgtcgact   7380

caggcgacga cggaattcct gcagcccatc tgcagaattc aggagagacc gggttggcgg   7440

cgtatttgtg tcccaaaaaa cagccccaat tgccccggag aagacggcca ggccgcctag   7500

atgacaaatt caacaactca cagctgactt tctgccattg ccactagggg ggggcctttt   7560

tatatggcca agccaagctc tccacgtcgg ttgggctgca cccaacaata aatgggtagg   7620

gttgcaccaa caaagggatg ggatgggggg tagaagatac gaggataacg gggctcaatg   7680

gcacaaataa gaacgaatac tgccattaag actcgtgatc cagcgactga caccattgca   7740

tcatctaagg gcctcaaaac tacctcggaa ctgctgcgct gatctggaca ccacagaggt   7800

tccgagcact ttaggttgca ccaaatgtcc caccaggtgc aggcagaaaa cgctggaaca   7860

gcgtgtacag tttgtcttaa caaaaagtga gggcgctgag gtcgagcagg gtggtgtgac   7920

ttgttatagc ctttagagct gcgaaagcgc gtatggattt ggctcatcag gccagattga   7980

gggtctgtgg acacatgtca tgttagtgta cttcaatcgc cccctggata tagccccgac   8040

aataggccgt ggcctcattt ttttgccttc cgcacatttc cattgctcgg tacccacacc   8100

ttgcttctcc tgcacttgcc aaccttaata ctggtttaca ttgaccaaca tcttacaagc   8160

ggggggcttg tctagggtat atataaacag tggctctccc aatcggttgc cagtctcttt   8220

tttcctttct ttccccacag attcgaaatc taaactacac atcacacaat gcctgttact   8280

gacgtcctta agcgaaagtc cggtgtcatc gtcggcgacg atgtccgagc cgtgagtatc   8340

cacgacaaga tcagtgtcga gacgacgcgt tttgtgtaat gacacaatcc gaaagtcgct   8400

agcaacacac actctctaca caaactaacc cagctctc                           8438


<210>  10
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  M13F universal primer

<400>  10
tgtaaaacga cggccagt                                                   18


<210>  11
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer M13-28Rev

<400>  11
gtaatacgac tcactatagg gc                                              22


<210>  12
<211>  1362
<212>  DNA
<213>  Euglena anabaena


<220>
<221>  misc_feature
<222>  (1)..(1362)
<223>  delta-5 desaturase

<400>  12
atggccacca tctctttgac tactgagcaa cttttagaac acccagaact ggttgcaatt     60

gatggggtgt tgtacgatct cttcggactg gcgaaagtgc atccaggtgg caacctcatt    120

gaagccgccg gtgcctccga cggaaccgcc ctgttctact ccatgcaccc tggagtgaag    180

ccagagaatt cgaagctgct gcagcaattt gcccgaggca aacacgaacg aagctcgaag    240

gacccagtgt acacctttga cagtcccttc gcccaggatg tcaagcagag cgttcgggag    300

gtcatgaagg ggcgcaactg gtacgccacg cccggctttt ggctgcggac cgcgctgatc    360

atcgcgtgca ctgccatagg cgaatggtat tggatcacta ccggggcagt gatgtggggc    420

atcttcaccg ggtacttcca cagccagatt gggttggcga ttcaacacga tgcctctcac    480

ggagccatca gcaaaaagcc ctgggtgaac gcctttttcg cctacggcat cgacgccatt    540

ggatcctccc gctggatctg gctgcagtcc cacattatgc gccaccacac ctacaccaac    600

cagcatggcc tggacctgga cgctgcctcg gcggagccgt tcattttgtt ccactcctac    660

ccggcaacaa atgcgtcacg aaagtggtac catcggttcc aggcgtggta catgtacatc    720

gttttgggga tgtatggtgt gtcgatggtg tacaatccga tgtacttgtt cacgatgcag    780

cacaacgaca caatcccaga ggccacctct cttagaccag gcagcttttt caaccggcag    840

cgcgccttcg ccgtttccct ccgcctactg ttcatcttcc gcaacgcctt cctcccctgg    900

tacatcgcgg gcgcctctcc gctgctcacc atcctgctgg tgccaacggt cacaggcatc    960

ttcttgacat ttgtttttgt gctgtcccat aactttgaag gcgctgagcg gacccccgaa   1020

aagaactgca aggccaaaag ggccaaggag gggaaggagg tccgcgatgt agaggaggac   1080

cgggtggact ggtaccgggc gcaggccgag accgcggcga cctacggggg cagcgtcggg   1140

atgatgctga ccggcggttt gaacctgcag atcgagcacc acttgttccc ccgcatgtcc   1200

tcttggcact accccttcat ccaagatacg gtgcgggaat gttgcaagcg ccatggcgtg   1260

cgctacacat actacccgac catcctggag aatataatgt ccacgctccg ctacatgcag   1320

aaggtgggcg tggcccacac aattcaggat gcccaggaat tc                      1362


<210>  13
<211>  454
<212>  PRT
<213>  Euglena anabaena

<400>  13

Met Ala Thr Ile Ser Leu Thr Thr Glu Gln Leu Leu Glu His Pro Glu 
1               5                   10                  15      


Leu Val Ala Ile Asp Gly Val Leu Tyr Asp Leu Phe Gly Leu Ala Lys 
            20                  25                  30          


Val His Pro Gly Gly Asn Leu Ile Glu Ala Ala Gly Ala Ser Asp Gly 
        35                  40                  45              


Thr Ala Leu Phe Tyr Ser Met His Pro Gly Val Lys Pro Glu Asn Ser 
    50                  55                  60                  


Lys Leu Leu Gln Gln Phe Ala Arg Gly Lys His Glu Arg Ser Ser Lys 
65                  70                  75                  80  


Asp Pro Val Tyr Thr Phe Asp Ser Pro Phe Ala Gln Asp Val Lys Gln 
                85                  90                  95      


Ser Val Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly 
            100                 105                 110         


Phe Trp Leu Arg Thr Ala Leu Ile Ile Ala Cys Thr Ala Ile Gly Glu 
        115                 120                 125             


Trp Tyr Trp Ile Thr Thr Gly Ala Val Met Trp Gly Ile Phe Thr Gly 
    130                 135                 140                 


Tyr Phe His Ser Gln Ile Gly Leu Ala Ile Gln His Asp Ala Ser His 
145                 150                 155                 160 


Gly Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Phe Phe Ala Tyr Gly 
                165                 170                 175     


Ile Asp Ala Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile 
            180                 185                 190         


Met Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala 
        195                 200                 205             


Ala Ser Ala Glu Pro Phe Ile Leu Phe His Ser Tyr Pro Ala Thr Asn 
    210                 215                 220                 


Ala Ser Arg Lys Trp Tyr His Arg Phe Gln Ala Trp Tyr Met Tyr Ile 
225                 230                 235                 240 


Val Leu Gly Met Tyr Gly Val Ser Met Val Tyr Asn Pro Met Tyr Leu 
                245                 250                 255     


Phe Thr Met Gln His Asn Asp Thr Ile Pro Glu Ala Thr Ser Leu Arg 
            260                 265                 270         


Pro Gly Ser Phe Phe Asn Arg Gln Arg Ala Phe Ala Val Ser Leu Arg 
        275                 280                 285             


Leu Leu Phe Ile Phe Arg Asn Ala Phe Leu Pro Trp Tyr Ile Ala Gly 
    290                 295                 300                 


Ala Ser Pro Leu Leu Thr Ile Leu Leu Val Pro Thr Val Thr Gly Ile 
305                 310                 315                 320 


Phe Leu Thr Phe Val Phe Val Leu Ser His Asn Phe Glu Gly Ala Glu 
                325                 330                 335     


Arg Thr Pro Glu Lys Asn Cys Lys Ala Lys Arg Ala Lys Glu Gly Lys 
            340                 345                 350         


Glu Val Arg Asp Val Glu Glu Asp Arg Val Asp Trp Tyr Arg Ala Gln 
        355                 360                 365             


Ala Glu Thr Ala Ala Thr Tyr Gly Gly Ser Val Gly Met Met Leu Thr 
    370                 375                 380                 


Gly Gly Leu Asn Leu Gln Ile Glu His His Leu Phe Pro Arg Met Ser 
385                 390                 395                 400 


Ser Trp His Tyr Pro Phe Ile Gln Asp Thr Val Arg Glu Cys Cys Lys 
                405                 410                 415     


Arg His Gly Val Arg Tyr Thr Tyr Tyr Pro Thr Ile Leu Glu Asn Ile 
            420                 425                 430         


Met Ser Thr Leu Arg Tyr Met Gln Lys Val Gly Val Ala His Thr Ile 
        435                 440                 445             


Gln Asp Ala Gln Glu Phe 
    450                 


<210>  14
<211>  4276
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  plasmid pLF119

<400>  14
gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac     60

tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta    120

ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta    180

cattgcacaa gataaaaata tatcatcatg ttagaaaaac tcatcgagca tcaaatgaaa    240

ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa    300

tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc    360

gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt    420

atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagcttatg    480

catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc    540

atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct    600

gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc    660

atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg ctgttttccc    720

ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt    780

cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt    840

ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa    900

tcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa    960

atcagcatcc atgttggaat ttaatcgcgg cctcgagcaa gacgtttccc gttgaatatg   1020

gctcatagat cttttctcca tcactgatag ggagtggtaa aataactcca tcaatgatag   1080

agtgtcaaca acatgaccaa aatcccttaa cgtgagttac gcgtattaat tgcgttgcgc   1140

tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa   1200

cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg   1260

ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg   1320

ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag   1380

gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac   1440

gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga   1500

taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt   1560

accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc   1620

tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc   1680

cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta   1740

agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat   1800

gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggttacac tagaagaaca   1860

gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct   1920

tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt   1980

acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct   2040

cagggaacga cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa aggccatccg   2100

tcaggatggc cttctgctta gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc   2160

accctccggg ccgttgcttc acaacgttca aatccgctcc cggcggattt gtcctactca   2220

ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtcttcc gactgagcct   2280

ttcgttttat ttgatgcctg gcagttccct actctcgcgt taacgctagc atggatgttt   2340

tcccagtcac gacgttgtaa aacgacggcc agtcttaagc tcgggcccca aataatgatt   2400

ttattttgac tgatagtgac ctgttcgttg caacaaattg atgagcaatg cttttttata   2460

atgccaactt tgtacaaaaa agttggattt tttttcggga tggccaccat ctctttgact   2520

actgagcaac ttttagaaca cccagaactg gttgcaattg atggggtgtt gtacgatctc   2580

ttcggactgg cgaaagtgca tccaggtggc aacctcattg aagccgccgg tgcctccgac   2640

ggaaccgccc tgttctactc catgcaccct ggagtgaagc cagagaattc gaagctgctg   2700

cagcaatttg cccgaggcaa acacgaacga agctcgaagg acccagtgta cacctttgac   2760

agtcccttcg cccaggatgt caagcagagc gttcgggagg tcatgaaggg gcgcaactgg   2820

tacgccacgc ccggcttttg gctgcggacc gcgctgatca tcgcgtgcac tgccataggc   2880

gaatggtatt ggatcactac cggggcagtg atgtggggca tcttcaccgg gtacttccac   2940

agccagattg ggttggcgat tcaacacgat gcctctcacg gagccatcag caaaaagccc   3000

tgggtgaacg cctttttcgc ctacggcatc gacgccattg gatcctcccg ctggatctgg   3060

ctgcagtccc acattatgcg ccaccacacc tacaccaacc agcatggcct ggacctggac   3120

gctgcctcgg cggagccgtt cattttgttc cactcctacc cggcaacaaa tgcgtcacga   3180

aagtggtacc atcggttcca ggcgtggtac atgtacatcg ttttggggat gtatggtgtg   3240

tcgatggtgt acaatccgat gtacttgttc acgatgcagc acaacgacac aatcccagag   3300

gccacctctc ttagaccagg cagctttttc aaccggcagc gcgccttcgc cgtttccctc   3360

cgcctactgt tcatcttccg caacgccttc ctcccctggt acatcgcggg cgcctctccg   3420

ctgctcacca tcctgctggt gccaacggtc acaggcatct tcttgacatt tgtttttgtg   3480

ctgtcccata actttgaagg cgctgagcgg acccccgaaa agaactgcaa ggccaaaagg   3540

gccaaggagg ggaaggaggt ccgcgatgta gaggaggacc gggtggactg gtaccgggcg   3600

caggccgaga ccgcggcgac ctacgggggc agcgtcggga tgatgctgac cggcggtttg   3660

aacctgcaga tcgagcacca cttgttcccc cgcatgtcct cttggcacta ccccttcatc   3720

caagatacgg tgcgggaatg ttgcaagcgc catggcgtgc gctacacata ctacccgacc   3780

atcctggaga atataatgtc cacgctccgc tacatgcaga aggtgggcgt ggcccacaca   3840

attcaggatg cccaggaatt ctgagtgagt tcgatccgca tcgacgtcta ccatttttga   3900

tgctgtctat tcctgttttc agtcacctcc agcattctca tggctggtga ccactgcccc   3960

tctaacccat tgtgacacac cgccaaagac tttgcctctt ttttttccct ttcttttgtc   4020

ctcggggtgc tttggccggt gtttactcgc cttgcagtcc ccgcaaacga ccgacgttta   4080

agctccgttg ttgactgggc cgctcgtaaa cccatctgca ggttgaggct cccatggaga   4140

attgtgatgg ctgattagga ggtggcgggg catacatgcc tcgacactca aagccgggcg   4200

gcttctggat tcgaaaacgc aaatgggcgc tttggaaaaa aaaaaaaaaa aaaaaaaaaa   4260

aaaacccaac tttctt                                                   4276


<210>  15
<211>  476
<212>  PRT
<213>  Thalassiosira pseudonana

<400>  15

Met Pro Pro Asn Ala Asp Ile Ser Arg Ile Arg Asn Arg Ile Pro Thr 
1               5                   10                  15      


Lys Thr Gly Thr Val Ala Ser Ala Asp Asn Asn Asp Pro Ala Thr Gln 
            20                  25                  30          


Ser Val Arg Thr Leu Lys Ser Leu Lys Gly Asn Glu Val Val Ile Asn 
        35                  40                  45              


Gly Thr Ile Tyr Asp Ile Ala Asp Phe Val His Pro Gly Gly Glu Val 
    50                  55                  60                  


Val Lys Phe Phe Gly Gly Asn Asp Val Thr Ile Gln Tyr Asn Met Ile 
65                  70                  75                  80  


His Pro Tyr His Thr Gly Lys His Leu Glu Lys Met Lys Ala Val Gly 
                85                  90                  95      


Lys Val Val Asp Trp Gln Ser Asp Tyr Lys Phe Asp Thr Pro Phe Glu 
            100                 105                 110         


Arg Glu Ile Lys Ser Glu Val Phe Lys Ile Val Arg Arg Gly Arg Glu 
        115                 120                 125             


Phe Gly Thr Thr Gly Tyr Phe Leu Arg Ala Phe Phe Tyr Ile Ala Leu 
    130                 135                 140                 


Phe Phe Thr Met Gln Tyr Thr Phe Ala Thr Cys Thr Thr Phe Thr Thr 
145                 150                 155                 160 


Tyr Asp His Trp Tyr Gln Ser Gly Val Phe Ile Ala Ile Val Phe Gly 
                165                 170                 175     


Ile Ser Gln Ala Phe Ile Gly Leu Asn Val Gln His Asp Ala Asn His 
            180                 185                 190         


Gly Ala Ala Ser Lys Arg Pro Trp Val Asn Asp Leu Leu Gly Phe Gly 
        195                 200                 205             


Thr Asp Leu Ile Gly Ser Asn Lys Trp Asn Trp Met Ala Gln His Trp 
    210                 215                 220                 


Thr His His Ala Tyr Thr Asn His Ser Glu Lys Asp Pro Asp Ser Phe 
225                 230                 235                 240 


Ser Ser Glu Pro Met Phe Ala Phe Asn Asp Tyr Pro Ile Gly His Pro 
                245                 250                 255     


Lys Arg Lys Trp Trp His Arg Phe Gln Gly Gly Tyr Phe Leu Phe Met 
            260                 265                 270         


Leu Gly Leu Tyr Trp Leu Pro Thr Val Phe Asn Pro Gln Phe Ile Asp 
        275                 280                 285             


Leu Arg Gln Arg Gly Ala Gln Tyr Val Gly Ile Gln Met Glu Asn Asp 
    290                 295                 300                 


Phe Ile Val Lys Arg Arg Lys Tyr Ala Val Ala Leu Arg Met Met Tyr 
305                 310                 315                 320 


Ile Tyr Leu Asn Ile Val Ser Pro Phe Met Asn Asn Gly Leu Ser Trp 
                325                 330                 335     


Ser Thr Phe Gly Ile Ile Met Leu Met Gly Ile Ser Glu Ser Leu Thr 
            340                 345                 350         


Leu Ser Val Leu Phe Ser Leu Ser His Asn Phe Ile Asn Ser Asp Arg 
        355                 360                 365             


Asp Pro Thr Ala Asp Phe Lys Lys Thr Gly Glu Gln Val Cys Trp Phe 
    370                 375                 380                 


Lys Ser Gln Val Glu Thr Ser Ser Thr Tyr Gly Gly Phe Ile Ser Gly 
385                 390                 395                 400 


Cys Leu Thr Gly Gly Leu Asn Phe Gln Val Glu His His Leu Phe Pro 
                405                 410                 415     


Arg Met Ser Ser Ala Trp Tyr Pro Tyr Ile Ala Pro Thr Val Arg Glu 
            420                 425                 430         


Val Cys Lys Lys His Gly Met Ser Tyr Ala Tyr Tyr Pro Trp Ile Gly 
        435                 440                 445             


Gln Asn Leu Val Ser Thr Phe Lys Tyr Met His Arg Ala Gly Ser Gly 
    450                 455                 460                 


Ala Asn Trp Glu Leu Lys Pro Leu Ser Gly Ser Ala 
465                 470                 475     


<210>  16
<211>  469
<212>  PRT
<213>  Phaeodactylum tricornutum

<400>  16

Met Ala Pro Asp Ala Asp Lys Leu Arg Gln Arg Gln Thr Thr Ala Val 
1               5                   10                  15      


Ala Lys His Asn Ala Ala Thr Ile Ser Thr Gln Glu Arg Leu Cys Ser 
            20                  25                  30          


Leu Ser Ser Leu Lys Gly Glu Glu Val Cys Ile Asp Gly Ile Ile Tyr 
        35                  40                  45              


Asp Leu Gln Ser Phe Asp His Pro Gly Gly Glu Thr Ile Lys Met Phe 
    50                  55                  60                  


Gly Gly Asn Asp Val Thr Val Gln Tyr Lys Met Ile His Pro Tyr His 
65                  70                  75                  80  


Thr Glu Lys His Leu Glu Lys Met Lys Arg Val Gly Lys Val Thr Asp 
                85                  90                  95      


Phe Val Cys Glu Tyr Lys Phe Asp Thr Glu Phe Glu Arg Glu Ile Lys 
            100                 105                 110         


Arg Glu Val Phe Lys Ile Val Arg Arg Gly Lys Asp Phe Gly Thr Leu 
        115                 120                 125             


Gly Trp Phe Phe Arg Ala Phe Cys Tyr Ile Ala Ile Phe Phe Tyr Leu 
    130                 135                 140                 


Gln Tyr His Trp Val Thr Thr Gly Thr Ser Trp Leu Leu Ala Val Ala 
145                 150                 155                 160 


Tyr Gly Ile Ser Gln Ala Met Ile Gly Met Asn Val Gln His Asp Ala 
                165                 170                 175     


Asn His Gly Ala Thr Ser Lys Arg Pro Trp Val Asn Asp Met Leu Gly 
            180                 185                 190         


Leu Gly Ala Asp Phe Ile Gly Gly Ser Lys Trp Leu Trp Gln Glu Gln 
        195                 200                 205             


His Trp Thr His His Ala Tyr Thr Asn His Ala Glu Met Asp Pro Asp 
    210                 215                 220                 


Ser Phe Gly Ala Glu Pro Met Leu Leu Phe Asn Asp Tyr Pro Leu Asp 
225                 230                 235                 240 


His Pro Ala Arg Thr Trp Leu His Arg Phe Gln Ala Phe Phe Tyr Met 
                245                 250                 255     


Pro Val Leu Ala Gly Tyr Trp Leu Ser Ala Val Phe Asn Pro Gln Ile 
            260                 265                 270         


Leu Asp Leu Gln Gln Arg Gly Ala Leu Ser Val Gly Ile Arg Leu Asp 
        275                 280                 285             


Asn Ala Phe Ile His Ser Arg Arg Lys Tyr Ala Val Phe Trp Arg Ala 
    290                 295                 300                 


Val Tyr Ile Ala Val Asn Val Ile Ala Pro Phe Tyr Thr Asn Ser Gly 
305                 310                 315                 320 


Leu Glu Trp Ser Trp Arg Val Phe Gly Asn Ile Met Leu Met Gly Val 
                325                 330                 335     


Ala Glu Ser Leu Ala Leu Ala Val Leu Phe Ser Leu Ser His Asn Phe 
            340                 345                 350         


Glu Ser Ala Asp Arg Asp Pro Thr Ala Pro Leu Lys Lys Thr Gly Glu 
        355                 360                 365             


Pro Val Asp Trp Phe Lys Thr Gln Val Glu Thr Ser Cys Thr Tyr Gly 
    370                 375                 380                 


Gly Phe Leu Ser Gly Cys Phe Thr Gly Gly Leu Asn Phe Gln Val Glu 
385                 390                 395                 400 


His His Leu Phe Pro Arg Met Ser Ser Ala Trp Tyr Pro Tyr Ile Ala 
                405                 410                 415     


Pro Lys Val Arg Glu Ile Cys Ala Lys His Gly Val His Tyr Ala Tyr 
            420                 425                 430         


Tyr Pro Trp Ile His Gln Asn Phe Leu Ser Thr Val Arg Tyr Met His 
        435                 440                 445             


Ala Ala Gly Thr Gly Ala Asn Trp Arg Gln Met Ala Arg Glu Asn Pro 
    450                 455                 460                 


Leu Thr Gly Arg Ala 
465                 


<210>  17
<211>  9472
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  plasmid pDMW263

<400>  17
catggcatgg atggtacgtc ctgtagaaac cccaacccgt gaaatcaaaa aactcgacgg     60

cctgtgggca ttcagtctgg atcgcgaaaa ctgtggaatt gatcagcgtt ggtgggaaag    120

cgcgttacaa gaaagccggg caattgctgt gccaggcagt tttaacgatc agttcgccga    180

tgcagatatt cgtaattatg cgggcaacgt ctggtatcag cgcgaagtct ttataccgaa    240

aggttgggca ggccagcgta tcgtgctgcg tttcgatgcg gtcactcatt acggcaaagt    300

gtgggtcaat aatcaggaag tgatggagca tcagggcggc tatacgccat ttgaagccga    360

tgtcacgccg tatgttattg ccgggaaaag tgtacgtatc accgtttgtg tgaacaacga    420

actgaactgg cagactatcc cgccgggaat ggtgattacc gacgaaaacg gcaagaaaaa    480

gcagtcttac ttccatgatt tctttaacta tgccgggatc catcgcagcg taatgctcta    540

caccacgccg aacacctggg tggacgatat caccgtggtg acgcatgtcg cgcaagactg    600

taaccacgcg tctgttgact ggcaggtggt ggccaatggt gatgtcagcg ttgaactgcg    660

tgatgcggat caacaggtgg ttgcaactgg acaaggcact agcgggactt tgcaagtggt    720

gaatccgcac ctctggcaac cgggtgaagg ttatctctat gaactgtgcg tcacagccaa    780

aagccagaca gagtgtgata tctacccgct tcgcgtcggc atccggtcag tggcagtgaa    840

gggcgaacag ttcctgatta accacaaacc gttctacttt actggctttg gtcgtcatga    900

agatgcggac ttacgtggca aaggattcga taacgtgctg atggtgcacg accacgcatt    960

aatggactgg attggggcca actcctaccg tacctcgcat tacccttacg ctgaagagat   1020

gctcgactgg gcagatgaac atggcatcgt ggtgattgat gaaactgctg ctgtcggctt   1080

taacctctct ttaggcattg gtttcgaagc gggcaacaag ccgaaagaac tgtacagcga   1140

agaggcagtc aacggggaaa ctcagcaagc gcacttacag gcgattaaag agctgatagc   1200

gcgtgacaaa aaccacccaa gcgtggtgat gtggagtatt gccaacgaac cggatacccg   1260

tccgcaagtg cacgggaata tttcgccact ggcggaagca acgcgtaaac tcgacccgac   1320

gcgtccgatc acctgcgtca atgtaatgtt ctgcgacgct cacaccgata ccatcagcga   1380

tctctttgat gtgctgtgcc tgaaccgtta ttacggatgg tatgtccaaa gcggcgattt   1440

ggaaacggca gagaaggtac tggaaaaaga acttctggcc tggcaggaga aactgcatca   1500

gccgattatc atcaccgaat acggcgtgga tacgttagcc gggctgcact caatgtacac   1560

cgacatgtgg agtgaagagt atcagtgtgc atggctggat atgtatcacc gcgtctttga   1620

tcgcgtcagc gccgtcgtcg gtgaacaggt atggaatttc gccgattttg cgacctcgca   1680

aggcatattg cgcgttggcg gtaacaagaa agggatcttc actcgcgacc gcaaaccgaa   1740

gtcggcggct tttctgctgc aaaaacgctg gactggcatg aacttcggtg aaaaaccgca   1800

gcagggaggc aaacaatgat taattaacta gagcggccgc caccgcggcc cgagattccg   1860

gcctcttcgg ccgccaagcg acccgggtgg acgtctagag gtacctagca attaacagat   1920

agtttgccgg tgataattct cttaacctcc cacactcctt tgacataacg atttatgtaa   1980

cgaaactgaa atttgaccag atattgtgtc cgcggtggag ctccagcttt tgttcccttt   2040

agtgagggtt aatttcgagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt   2100

gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg   2160

gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt   2220

cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt   2280

tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc   2340

tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg   2400

ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg   2460

ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac   2520

gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg   2580

gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct   2640

ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg   2700

tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct   2760

gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac   2820

tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt   2880

tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc   2940

tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca   3000

ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat   3060

ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac   3120

gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt   3180

aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc   3240

aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg   3300

cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg   3360

ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc   3420

cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta   3480

ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg   3540

ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct   3600

ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta   3660

gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg   3720

ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga   3780

ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt   3840

gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca   3900

ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt   3960

cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt   4020

ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga   4080

aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt   4140

gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc   4200

gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta agcgcggcgg   4260

gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt   4320

tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc   4380

gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg   4440

attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga   4500

cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc   4560

ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa   4620

aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa   4680

tttccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc   4740

gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc   4800

agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattgtaat acgactcact   4860

atagggcgaa ttgggtaccg ggccccccct cgaggtcgat ggtgtcgata agcttgatat   4920

cgaattcatg tcacacaaac cgatcttcgc ctcaaggaaa cctaattcta catccgagag   4980

actgccgaga tccagtctac actgattaat tttcgggcca ataatttaaa aaaatcgtgt   5040

tatataatat tatatgtatt atatatatac atcatgatga tactgacagt catgtcccat   5100

tgctaaatag acagactcca tctgccgcct ccaactgatg ttctcaatat ttaaggggtc   5160

atctcgcatt gtttaataat aaacagactc catctaccgc ctccaaatga tgttctcaaa   5220

atatattgta tgaacttatt tttattactt agtattatta gacaacttac ttgctttatg   5280

aaaaacactt cctatttagg aaacaattta taatggcagt tcgttcattt aacaatttat   5340

gtagaataaa tgttataaat gcgtatggga aatcttaaat atggatagca taaatgatat   5400

ctgcattgcc taattcgaaa tcaacagcaa cgaaaaaaat cccttgtaca acataaatag   5460

tcatcgagaa atatcaacta tcaaagaaca gctattcaca cgttactatt gagattatta   5520

ttggacgaga atcacacact caactgtctt tctctcttct agaaatacag gtacaagtat   5580

gtactattct cattgttcat acttctagtc atttcatccc acatattcct tggatttctc   5640

tccaatgaat gacattctat cttgcaaatt caacaattat aataagatat accaaagtag   5700

cggtatagtg gcaatcaaaa agcttctctg gtgtgcttct cgtatttatt tttattctaa   5760

tgatccatta aaggtatata tttatttctt gttatataat ccttttgttt attacatggg   5820

ctggatacat aaaggtattt tgatttaatt ttttgcttaa attcaatccc ccctcgttca   5880

gtgtcaactg taatggtagg aaattaccat acttttgaag aagcaaaaaa aatgaaagaa   5940

aaaaaaaatc gtatttccag gttagacgtt ccgcagaatc tagaatgcgg tatgcggtac   6000

attgttcttc gaacgtaaaa gttgcgctcc ctgagatatt gtacattttt gcttttacaa   6060

gtacaagtac atcgtacaac tatgtactac tgttgatgca tccacaacag tttgttttgt   6120

ttttttttgt tttttttttt tctaatgatt cattaccgct atgtatacct acttgtactt   6180

gtagtaagcc gggttattgg cgttcaatta atcatagact tatgaatctg cacggtgtgc   6240

gctgcgagtt acttttagct tatgcatgct acttgggtgt aatattggga tctgttcgga   6300

aatcaacgga tgctcaaccg atttcgacag taataatttg aatcgaatcg gagcctaaaa   6360

tgaacccgag tatatctcat aaaattctcg gtgagaggtc tgtgactgtc agtacaaggt   6420

gccttcatta tgccctcaac cttaccatac ctcactgaat gtagtgtacc tctaaaaatg   6480

aaatacagtg ccaaaagcca aggcactgag ctcgtctaac ggacttgata tacaaccaat   6540

taaaacaaat gaaaagaaat acagttcttt gtatcatttg taacaattac cctgtacaaa   6600

ctaaggtatt gaaatcccac aatattccca aagtccaccc ctttccaaat tgtcatgcct   6660

acaactcata taccaagcac taacctacca aacaccacta aaaccccaca aaatatatct   6720

taccgaatat acagtaacaa gctaccacca cactcgttgg gtgcagtcgc cagcttaaag   6780

atatctatcc acatcagcca caactccctt cctttaataa accgactaca cccttggcta   6840

ttgaggttat gagtgaatat actgtagaca agacactttc aagaagactg tttccaaaac   6900

gtaccactgt cctccactac aaacacaccc aatctgcttc ttctagtcaa ggttgctaca   6960

ccggtaaatt ataaatcatc atttcattag cagggcaggg ccctttttat agagtcttat   7020

acactagcgg accctgccgg tagaccaacc cgcaggcgcg tcagtttgct ccttccatca   7080

atgcgtcgta gaaacgactt actccttctt gagcagctcc ttgaccttgt tggcaacaag   7140

tctccgacct cggaggtgga ggaagagcct ccgatatcgg cggtagtgat accagcctcg   7200

acggactcct tgacggcagc ctcaacagcg tcaccggcgg gcttcatgtt aagagagaac   7260

ttgagcatca tggcggcaga cagaatggtg gcaatggggt tgaccttctg cttgccgaga   7320

tcgggggcag atccgtgaca gggctcgtac agaccgaacg cctcgttggt gtcgggcaga   7380

gaagccagag aggcggaggg cagcagaccc agagaaccgg ggatgacgga ggcctcgtcg   7440

gagatgatat cgccaaacat gttggtggtg atgatgatac cattcatctt ggagggctgc   7500

ttgatgagga tcatggcggc cgagtcgatc agctggtggt tgagctcgag ctgggggaat   7560

tcgtccttga ggactcgagt gacagtcttt cgccaaagtc gagaggaggc cagcacgttg   7620

gccttgtcaa gagaccacac gggaagaggg gggttgtgct gaagggccag gaaggcggcc   7680

attcgggcaa ttcgctcaac ctcaggaacg gagtaggtct cggtgtcgga agcgacgcca   7740

gatccgtcat cctcctttcg ctctccaaag tagatacctc cgacgagctc tcggacaatg   7800

atgaagtcgg tgccctcaac gtttcggatg ggggagagat cggcgagctt gggcgacagc   7860

agctggcagg gtcgcaggtt ggcgtacagg ttcaggtcct ttcgcagctt gaggagaccc   7920

tgctcgggtc gcacgtcggt tcgtccgtcg ggagtggtcc atacggtgtt ggcagcgcct   7980

ccgacagcac cgagcataat agagtcagcc tttcggcaga tgtcgagagt agcgtcggtg   8040

atgggctcgc cctccttctc aatggcagct cctccaatga gtcggtcctc aaacacaaac   8100

tcggtgccgg aggcctcagc aacagacttg agcaccttga cggcctcggc aatcacctcg   8160

gggccacaga agtcgccgcc gagaagaaca atcttcttgg agtcagtctt ggtcttctta   8220

gtttcgggtt ccattgtgga tgtgtgtggt tgtatgtgtg atgtggtgtg tggagtgaaa   8280

atctgtggct ggcaaacgct cttgtatata tacgcacttt tgcccgtgct atgtggaaga   8340

ctaaacctcc gaagattgtg actcaggtag tgcggtatcg gctagggacc caaaccttgt   8400

cgatgccgat agcgctatcg aacgtacccc agccggccgg gagtatgtcg gaggggacat   8460

acgagatcgt caagggtttg tggccaactg gtaaataaat gatgtcgacg tttaaacagt   8520

gtacgcagat ctactataga ggaacattta aattgccccg gagaagacgg ccaggccgcc   8580

tagatgacaa attcaacaac tcacagctga ctttctgcca ttgccactag gggggggcct   8640

ttttatatgg ccaagccaag ctctccacgt cggttgggct gcacccaaca ataaatgggt   8700

agggttgcac caacaaaggg atgggatggg gggtagaaga tacgaggata acggggctca   8760

atggcacaaa taagaacgaa tactgccatt aagactcgtg atccagcgac tgacaccatt   8820

gcatcatcta agggcctcaa aactacctcg gaactgctgc gctgatctgg acaccacaga   8880

ggttccgagc actttaggtt gcaccaaatg tcccaccagg tgcaggcaga aaacgctgga   8940

acagcgtgta cagtttgtct taacaaaaag tgagggcgct gaggtcgagc agggtggtgt   9000

gacttgttat agcctttaga gctgcgaaag cgcgtatgga tttggctcat caggccagat   9060

tgagggtctg tggacacatg tcatgttagt gtacttcaat cgccccctgg atatagcccc   9120

gacaataggc cgtggcctca tttttttgcc ttccgcacat ttccattgct cgatacccac   9180

accttgcttc tcctgcactt gccaacctta atactggttt acattgacca acatcttaca   9240

agcggggggc ttgtctaggg tatatataaa cagtggctct cccaatcggt tgccagtctc   9300

ttttttcctt tctttcccca cagattcgaa atctaaacta cacatcacag aattccgagc   9360

cgtgagtatc cacgacaaga tcagtgtcga gacgacgcgt tttgtgtaat gacacaatcc   9420

gaaagtcgct agcaacacac actctctaca caaactaacc cagctctggt ac           9472


<210>  18
<211>  7879
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  plasmid pDMW237

<400>  18
ggccgcaagt gtggatgggg aagtgagtgc ccggttctgt gtgcacaatt ggcaatccaa     60

gatggatgga ttcaacacag ggatatagcg agctacgtgg tggtgcgagg atatagcaac    120

ggatatttat gtttgacact tgagaatgta cgatacaagc actgtccaag tacaatacta    180

aacatactgt acatactcat actcgtaccc gggcaacggt ttcacttgag tgcagtggct    240

agtgctctta ctcgtacagt gtgcaatact gcgtatcata gtctttgatg tatatcgtat    300

tcattcatgt tagttgcgta cgagccggaa gcataaagtg taaagcctgg ggtgcctaat    360

gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc    420

tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg    480

ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag    540

cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag    600

gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc    660

tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc    720

agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc    780

tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt    840

cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg    900

ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat    960

ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag   1020

ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt   1080

ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc   1140

cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta   1200

gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag   1260

atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga   1320

ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa   1380

gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa   1440

tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc   1500

ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga   1560

taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa   1620

gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt   1680

gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg   1740

ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc   1800

aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg   1860

gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag   1920

cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt   1980

actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt   2040

caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac   2100

gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac   2160

ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag   2220

caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa   2280

tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga   2340

gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc   2400

cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg   2460

ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct   2520

tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc   2580

ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg   2640

atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt   2700

ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg   2760

tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc   2820

tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttccattc   2880

gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg   2940

ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc   3000

ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga   3060

attgggtacc gggccccccc tcgaggtcga tggtgtcgat aagcttgata tcgaattcat   3120

gtcacacaaa ccgatcttcg cctcaaggaa acctaattct acatccgaga gactgccgag   3180

atccagtcta cactgattaa ttttcgggcc aataatttaa aaaaatcgtg ttatataata   3240

ttatatgtat tatatatata catcatgatg atactgacag tcatgtccca ttgctaaata   3300

gacagactcc atctgccgcc tccaactgat gttctcaata tttaaggggt catctcgcat   3360

tgtttaataa taaacagact ccatctaccg cctccaaatg atgttctcaa aatatattgt   3420

atgaacttat ttttattact tagtattatt agacaactta cttgctttat gaaaaacact   3480

tcctatttag gaaacaattt ataatggcag ttcgttcatt taacaattta tgtagaataa   3540

atgttataaa tgcgtatggg aaatcttaaa tatggatagc ataaatgata tctgcattgc   3600

ctaattcgaa atcaacagca acgaaaaaaa tcccttgtac aacataaata gtcatcgaga   3660

aatatcaact atcaaagaac agctattcac acgttactat tgagattatt attggacgag   3720

aatcacacac tcaactgtct ttctctcttc tagaaataca ggtacaagta tgtactattc   3780

tcattgttca tacttctagt catttcatcc cacatattcc ttggatttct ctccaatgaa   3840

tgacattcta tcttgcaaat tcaacaatta taataagata taccaaagta gcggtatagt   3900

ggcaatcaaa aagcttctct ggtgtgcttc tcgtatttat ttttattcta atgatccatt   3960

aaaggtatat atttatttct tgttatataa tccttttgtt tattacatgg gctggataca   4020

taaaggtatt ttgatttaat tttttgctta aattcaatcc cccctcgttc agtgtcaact   4080

gtaatggtag gaaattacca tacttttgaa gaagcaaaaa aaatgaaaga aaaaaaaaat   4140

cgtatttcca ggttagacgt tccgcagaat ctagaatgcg gtatgcggta cattgttctt   4200

cgaacgtaaa agttgcgctc cctgagatat tgtacatttt tgcttttaca agtacaagta   4260

catcgtacaa ctatgtacta ctgttgatgc atccacaaca gtttgttttg tttttttttg   4320

tttttttttt ttctaatgat tcattaccgc tatgtatacc tacttgtact tgtagtaagc   4380

cgggttattg gcgttcaatt aatcatagac ttatgaatct gcacggtgtg cgctgcgagt   4440

tacttttagc ttatgcatgc tacttgggtg taatattggg atctgttcgg aaatcaacgg   4500

atgctcaatc gatttcgaca gtaattaatt aagtcataca caagtcagct ttcttcgagc   4560

ctcatataag tataagtagt tcaacgtatt agcactgtac ccagcatctc cgtatcgaga   4620

aacacaacaa catgccccat tggacagatc atgcggatac acaggttgtg cagtatcata   4680

catactcgat cagacaggtc gtctgaccat catacaagct gaacaagcgc tccatacttg   4740

cacgctctct atatacacag ttaaattaca tatccatagt ctaacctcta acagttaatc   4800

ttctggtaag cctcccagcc agccttctgg tatcgcttgg cctcctcaat aggatctcgg   4860

ttctggccgt acagacctcg gccgacaatt atgatatccg ttccggtaga catgacatcc   4920

tcaacagttc ggtactgctg tccgagagcg tctcccttgt cgtcaagacc caccccgggg   4980

gtcagaataa gccagtcctc agagtcgccc ttaggtcggt tctgggcaat gaagccaacc   5040

acaaactcgg ggtcggatcg ggcaagctca atggtctgct tggagtactc gccagtggcc   5100

agagagccct tgcaagacag ctcggccagc atgagcagac ctctggccag cttctcgttg   5160

ggagagggga ctaggaactc cttgtactgg gagttctcgt agtcagagac gtcctccttc   5220

ttctgttcag agacagtttc ctcggcacca gctcgcaggc cagcaatgat tccggttccg   5280

ggtacaccgt gggcgttggt gatatcggac cactcggcga ttcggtgaca ccggtactgg   5340

tgcttgacag tgttgccaat atctgcgaac tttctgtcct cgaacaggaa gaaaccgtgc   5400

ttaagagcaa gttccttgag ggggagcaca gtgccggcgt aggtgaagtc gtcaatgatg   5460

tcgatatggg ttttgatcat gcacacataa ggtccgacct tatcggcaag ctcaatgagc   5520

tccttggtgg tggtaacatc cagagaagca cacaggttgg ttttcttggc tgccacgagc   5580

ttgagcactc gagcggcaaa ggcggacttg tggacgttag ctcgagcttc gtaggagggc   5640

attttggtgg tgaagaggag actgaaataa atttagtctg cagaactttt tatcggaacc   5700

ttatctgggg cagtgaagta tatgttatgg taatagttac gagttagttg aacttataga   5760

tagactggac tatacggcta tcggtccaaa ttagaaagaa cgtcaatggc tctctgggcg   5820

tcgcctttgc cgacaaaaat gtgatcatga tgaaagccag caatgacgtt gcagctgata   5880

ttgttgtcgg ccaaccgcgc cgaaaacgca gctgtcagac ccacagcctc caacgaagaa   5940

tgtatcgtca aagtgatcca agcacactca tagttggagt cgtactccaa aggcggcaat   6000

gacgagtcag acagatactc gtcgactcag gcgacgacgg aattcctgca gcccatctgc   6060

agaattcagg agagaccggg ttggcggcgt atttgtgtcc caaaaaacag ccccaattgc   6120

cccggagaag acggccaggc cgcctagatg acaaattcaa caactcacag ctgactttct   6180

gccattgcca ctaggggggg gcctttttat atggccaagc caagctctcc acgtcggttg   6240

ggctgcaccc aacaataaat gggtagggtt gcaccaacaa agggatggga tggggggtag   6300

aagatacgag gataacgggg ctcaatggca caaataagaa cgaatactgc cattaagact   6360

cgtgatccag cgactgacac cattgcatca tctaagggcc tcaaaactac ctcggaactg   6420

ctgcgctgat ctggacacca cagaggttcc gagcacttta ggttgcacca aatgtcccac   6480

caggtgcagg cagaaaacgc tggaacagcg tgtacagttt gtcttaacaa aaagtgaggg   6540

cgctgaggtc gagcagggtg gtgtgacttg ttatagcctt tagagctgcg aaagcgcgta   6600

tggatttggc tcatcaggcc agattgaggg tctgtggaca catgtcatgt tagtgtactt   6660

caatcgcccc ctggatatag ccccgacaat aggccgtggc ctcatttttt tgccttccgc   6720

acatttccat tgctcggtac ccacaccttg cttctcctgc acttgccaac cttaatactg   6780

gtttacattg accaacatct tacaagcggg gggcttgtct agggtatata taaacagtgg   6840

ctctcccaat cggttgccag tctctttttt cctttctttc cccacagatt cgaaatctaa   6900

actacacatc acacaatgcc tgttactgac gtccttaagc gaaagtccgg tgtcatcgtc   6960

ggcgacgatg tccgagccgt gagtatccac gacaagatca gtgtcgagac gacgcgtttt   7020

gtgtaatgac acaatccgaa agtcgctagc aacacacact ctctacacaa actaacccag   7080

ctctccatgg ctctggccaa cgacgctggc gagcgaatct gggctgccgt caccgatccc   7140

gaaatcctca ttggcacctt ctcctacctg ctcctgaagc ctctcctgcg aaactctggt   7200

ctcgtggacg agaagaaagg agcctaccga acctccatga tctggtacaa cgtcctcctg   7260

gctctcttct ctgccctgtc cttctacgtg actgccaccg ctctcggctg ggactacggt   7320

actggagcct ggctgcgaag acagaccggt gatactcccc agcctctctt tcagtgtccc   7380

tctcctgtct gggactccaa gctgttcacc tggactgcca aggccttcta ctattctaag   7440

tacgtggagt acctcgacac cgcttggctg gtcctcaagg gcaagcgagt gtcctttctg   7500

caggccttcc atcactttgg agctccctgg gacgtctacc tcggcattcg actgcacaac   7560

gagggtgtgt ggatcttcat gttctttaac tcgttcattc acaccatcat gtacacctac   7620

tatggactga ctgccgctgg ctacaagttc aaggccaagc ctctgatcac tgccatgcag   7680

atttgccagt tcgtcggtgg ctttctcctg gtctgggact acatcaacgt tccctgcttc   7740

aactctgaca agggcaagct gttctcctgg gctttcaact acgcctacgt cggatctgtc   7800

tttctcctgt tctgtcactt cttttaccag gacaacctgg ccaccaagaa atccgctaag   7860

gctggtaagc agctttagc                                                7879


<210>  19
<211>  7783
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  plasmid pY115

<400>  19
catggctctg gccaacgacg ctggcgagcg aatctgggct gccgtcaccg atcccgaaat     60

cctcattggc accttctcct acctgctcct gaagcctctc ctgcgaaact ctggtctcgt    120

ggacgagaag aaaggagcct accgaacctc catgatctgg tacaacgtcc tcctggctct    180

cttctctgcc ctgtccttct acgtgactgc caccgctctc ggctgggact acggtactgg    240

agcctggctg cgaagacaga ccggtgatac tccccagcct ctctttcagt gtccctctcc    300

tgtctgggac tccaagctgt tcacctggac tgccaaggcc ttctactatt ctaagtacgt    360

ggagtacctc gacaccgctt ggctggtcct caagggcaag cgagtgtcct ttctgcaggc    420

cttccatcac tttggagctc cctgggacgt ctacctcggc attcgactgc acaacgaggg    480

tgtgtggatc ttcatgttct ttaactcgtt cattcacacc atcatgtaca cctactatgg    540

actgactgcc gctggctaca agttcaaggc caagcctctg atcactgcca tgcagatttg    600

ccagttcgtc ggtggctttc tcctggtctg ggactacatc aacgttccct gcttcaactc    660

tgacaagggc aagctgttct cctgggcttt caactacgcc tacgtcggat ctgtctttct    720

cctgttctgt cacttctttt accaggacaa cctggccacc aagaaatccg ctaaggctgg    780

taagcagctt tagcggccgc aagtgtggat ggggaagtga gtgcccggtt ctgtgtgcac    840

aattggcaat ccaagatgga tggattcaac acagggatat agcgagctac gtggtggtgc    900

gaggatatag caacggatat ttatgtttga cacttgagaa tgtacgatac aagcactgtc    960

caagtacaat actaaacata ctgtacatac tcatactcgt acccgggcaa cggtttcact   1020

tgagtgcagt ggctagtgct cttactcgta cagtgtgcaa tactgcgtat catagtcttt   1080

gatgtatatc gtattcattc atgttagttg cgtacgagcc ggaagcataa agtgtaaagc   1140

ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt   1200

ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg   1260

cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt   1320

tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc   1380

aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa   1440

aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa   1500

tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc   1560

ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc   1620

cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag   1680

ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga   1740

ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc   1800

gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac   1860

agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg   1920

cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca   1980

aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa   2040

aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa   2100

ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt   2160

aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag   2220

ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat   2280

agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc   2340

cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa   2400

ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca   2460

gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa   2520

cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt   2580

cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc   2640

ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact   2700

catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc   2760

tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg   2820

ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct   2880

catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc   2940

cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag   3000

cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac   3060

acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg   3120

ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt   3180

tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc   3240

ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc   3300

tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct   3360

aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa   3420

acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc   3480

tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact   3540

caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg   3600

gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct   3660

tacaatttcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc   3720

tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta   3780

acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac   3840

tcactatagg gcgaattggg taccgggccc cccctcgagg tcgatggtgt cgataagctt   3900

gatatcgaat tcatgtcaca caaaccgatc ttcgcctcaa ggaaacctaa ttctacatcc   3960

gagagactgc cgagatccag tctacactga ttaattttcg ggccaataat ttaaaaaaat   4020

cgtgttatat aatattatat gtattatata tatacatcat gatgatactg acagtcatgt   4080

cccattgcta aatagacaga ctccatctgc cgcctccaac tgatgttctc aatatttaag   4140

gggtcatctc gcattgttta ataataaaca gactccatct accgcctcca aatgatgttc   4200

tcaaaatata ttgtatgaac ttatttttat tacttagtat tattagacaa cttacttgct   4260

ttatgaaaaa cacttcctat ttaggaaaca atttataatg gcagttcgtt catttaacaa   4320

tttatgtaga ataaatgtta taaatgcgta tgggaaatct taaatatgga tagcataaat   4380

gatatctgca ttgcctaatt cgaaatcaac agcaacgaaa aaaatccctt gtacaacata   4440

aatagtcatc gagaaatatc aactatcaaa gaacagctat tcacacgtta ctattgagat   4500

tattattgga cgagaatcac acactcaact gtctttctct cttctagaaa tacaggtaca   4560

agtatgtact attctcattg ttcatacttc tagtcatttc atcccacata ttccttggat   4620

ttctctccaa tgaatgacat tctatcttgc aaattcaaca attataataa gatataccaa   4680

agtagcggta tagtggcaat caaaaagctt ctctggtgtg cttctcgtat ttatttttat   4740

tctaatgatc cattaaaggt atatatttat ttcttgttat ataatccttt tgtttattac   4800

atgggctgga tacataaagg tattttgatt taattttttg cttaaattca atcccccctc   4860

gttcagtgtc aactgtaatg gtaggaaatt accatacttt tgaagaagca aaaaaaatga   4920

aagaaaaaaa aaatcgtatt tccaggttag acgttccgca gaatctagaa tgcggtatgc   4980

ggtacattgt tcttcgaacg taaaagttgc gctccctgag atattgtaca tttttgcttt   5040

tacaagtaca agtacatcgt acaactatgt actactgttg atgcatccac aacagtttgt   5100

tttgtttttt tttgtttttt ttttttctaa tgattcatta ccgctatgta tacctacttg   5160

tacttgtagt aagccgggtt attggcgttc aattaatcat agacttatga atctgcacgg   5220

tgtgcgctgc gagttacttt tagcttatgc atgctacttg ggtgtaatat tgggatctgt   5280

tcggaaatca acggatgctc aatcgatttc gacagtaatt aattaagtca tacacaagtc   5340

agctttcttc gagcctcata taagtataag tagttcaacg tattagcact gtacccagca   5400

tctccgtatc gagaaacaca acaacatgcc ccattggaca gatcatgcgg atacacaggt   5460

tgtgcagtat catacatact cgatcagaca ggtcgtctga ccatcataca agctgaacaa   5520

gcgctccata cttgcacgct ctctatatac acagttaaat tacatatcca tagtctaacc   5580

tctaacagtt aatcttctgg taagcctccc agccagcctt ctggtatcgc ttggcctcct   5640

caataggatc tcggttctgg ccgtacagac ctcggccgac aattatgata tccgttccgg   5700

tagacatgac atcctcaaca gttcggtact gctgtccgag agcgtctccc ttgtcgtcaa   5760

gacccacccc gggggtcaga ataagccagt cctcagagtc gcccttaggt cggttctggg   5820

caatgaagcc aaccacaaac tcggggtcgg atcgggcaag ctcaatggtc tgcttggagt   5880

actcgccagt ggccagagag cccttgcaag acagctcggc cagcatgagc agacctctgg   5940

ccagcttctc gttgggagag gggactagga actccttgta ctgggagttc tcgtagtcag   6000

agacgtcctc cttcttctgt tcagagacag tttcctcggc accagctcgc aggccagcaa   6060

tgattccggt tccgggtaca ccgtgggcgt tggtgatatc ggaccactcg gcgattcggt   6120

gacaccggta ctggtgcttg acagtgttgc caatatctgc gaactttctg tcctcgaaca   6180

ggaagaaacc gtgcttaaga gcaagttcct tgagggggag cacagtgccg gcgtaggtga   6240

agtcgtcaat gatgtcgata tgggttttga tcatgcacac ataaggtccg accttatcgg   6300

caagctcaat gagctccttg gtggtggtaa catccagaga agcacacagg ttggttttct   6360

tggctgccac gagcttgagc actcgagcgg caaaggcgga cttgtggacg ttagctcgag   6420

cttcgtagga gggcattttg gtggtgaaga ggagactgaa ataaatttag tctgcagaac   6480

tttttatcgg aaccttatct ggggcagtga agtatatgtt atggtaatag ttacgagtta   6540

gttgaactta tagatagact ggactatacg gctatcggtc caaattagaa agaacgtcaa   6600

tggctctctg ggcgtcgcct ttgccgacaa aaatgtgatc atgatgaaag ccagcaatga   6660

cgttgcagct gatattgttg tcggccaacc gcgccgaaaa cgcagctgtc agacccacag   6720

cctccaacga agaatgtatc gtcaaagtga tccaagcaca ctcatagttg gagtcgtact   6780

ccaaaggcgg caatgacgag tcagacagat actcgtcgac gtttaaacag tgtacgcaga   6840

tctactatag aggaacattt aaattgcccc ggagaagacg gccaggccgc ctagatgaca   6900

aattcaacaa ctcacagctg actttctgcc attgccacta ggggggggcc tttttatatg   6960

gccaagccaa gctctccacg tcggttgggc tgcacccaac aataaatggg tagggttgca   7020

ccaacaaagg gatgggatgg ggggtagaag atacgaggat aacggggctc aatggcacaa   7080

ataagaacga atactgccat taagactcgt gatccagcga ctgacaccat tgcatcatct   7140

aagggcctca aaactacctc ggaactgctg cgctgatctg gacaccacag aggttccgag   7200

cactttaggt tgcaccaaat gtcccaccag gtgcaggcag aaaacgctgg aacagcgtgt   7260

acagtttgtc ttaacaaaaa gtgagggcgc tgaggtcgag cagggtggtg tgacttgtta   7320

tagcctttag agctgcgaaa gcgcgtatgg atttggctca tcaggccaga ttgagggtct   7380

gtggacacat gtcatgttag tgtacttcaa tcgccccctg gatatagccc cgacaatagg   7440

ccgtggcctc atttttttgc cttccgcaca tttccattgc tcgataccca caccttgctt   7500

ctcctgcact tgccaacctt aatactggtt tacattgacc aacatcttac aagcgggggg   7560

cttgtctagg gtatatataa acagtggctc tcccaatcgg ttgccagtct cttttttcct   7620

ttctttcccc acagattcga aatctaaact acacatcaca gaattccgag ccgtgagtat   7680

ccacgacaag atcagtgtcg agacgacgcg ttttgtgtaa tgacacaatc cgaaagtcgc   7740

tagcaacaca cactctctac acaaactaac ccagctctgg tac                     7783


<210>  20
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide oYFBA1

<400>  20
acgcagatct actatagag                                                  19


<210>  21
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide oYFBA1-6

<400>  21
agcggccgct ggtaccagag ctgggtt                                         27


<210>  22
<211>  6992
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  plasmid pY158

<400>  22
ggccgcaagt gtggatgggg aagtgagtgc ccggttctgt gtgcacaatt ggcaatccaa     60

gatggatgga ttcaacacag ggatatagcg agctacgtgg tggtgcgagg atatagcaac    120

ggatatttat gtttgacact tgagaatgta cgatacaagc actgtccaag tacaatacta    180

aacatactgt acatactcat actcgtaccc ggcaacggtt tcacttgagt gcagtggcta    240

gtgctcttac tcgtacagtg tgcaatactg cgtatcatag tctttgatgt atatcgtatt    300

cattcatgtt agttgcgtac gagccggaag cataaagtgt aaagcctggg gtgcctaatg    360

agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct    420

gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg    480

gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc    540

ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg    600

aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct    660

ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca    720

gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct    780

cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc    840

gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt    900

tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc    960

cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc   1020

cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg   1080

gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc   1140

agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag   1200

cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga   1260

tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat   1320

tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag   1380

ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat   1440

cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc   1500

cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat   1560

accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag   1620

ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg   1680

ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc   1740

tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca   1800

acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg   1860

tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc   1920

actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta   1980

ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc   2040

aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg   2100

ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc   2160

cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc   2220

aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat   2280

actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag   2340

cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc   2400

ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt   2460

tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt   2520

cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc   2580

tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga   2640

tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc   2700

cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt   2760

ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct   2820

gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttccattcg   2880

ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc   2940

cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc   3000

cagtcacgac gttgtaaaac gacggccagt gaattgtaat acgactcact atagggcgaa   3060

ttgggtaccg ggccccccct cgaggtcgat ggtgtcgata agcttgatat cgaattcatg   3120

tcacacaaac cgatcttcgc ctcaaggaaa cctaattcta catccgagag actgccgaga   3180

tccagtctac actgattaat tttcgggcca ataatttaaa aaaatcgtgt tatataatat   3240

tatatgtatt atatatatac atcatgatga tactgacagt catgtcccat tgctaaatag   3300

acagactcca tctgccgcct ccaactgatg ttctcaatat ttaaggggtc atctcgcatt   3360

gtttaataat aaacagactc catctaccgc ctccaaatga tgttctcaaa atatattgta   3420

tgaacttatt tttattactt agtattatta gacaacttac ttgctttatg aaaaacactt   3480

cctatttagg aaacaattta taatggcagt tcgttcattt aacaatttat gtagaataaa   3540

tgttataaat gcgtatggga aatcttaaat atggatagca taaatgatat ctgcattgcc   3600

taattcgaaa tcaacagcaa cgaaaaaaat cccttgtaca acataaatag tcatcgagaa   3660

atatcaacta tcaaagaaca gctattcaca cgttactatt gagattatta ttggacgaga   3720

atcacacact caactgtctt tctctcttct agaaatacag gtacaagtat gtactattct   3780

cattgttcat acttctagtc atttcatccc acatattcct tggatttctc tccaatgaat   3840

gacattctat cttgcaaatt caacaattat aataagatat accaaagtag cggtatagtg   3900

gcaatcaaaa agcttctctg gtgtgcttct cgtatttatt tttattctaa tgatccatta   3960

aaggtatata tttatttctt gttatataat ccttttgttt attacatggg ctggatacat   4020

aaaggtattt tgatttaatt ttttgcttaa attcaatccc ccctcgttca gtgtcaactg   4080

taatggtagg aaattaccat acttttgaag aagcaaaaaa aatgaaagaa aaaaaaaatc   4140

gtatttccag gttagacgtt ccgcagaatc tagaatgcgg tatgcggtac attgttcttc   4200

gaacgtaaaa gttgcgctcc ctgagatatt gtacattttt gcttttacaa gtacaagtac   4260

atcgtacaac tatgtactac tgttgatgca tccacaacag tttgttttgt ttttttttgt   4320

tttttttttt tctaatgatt cattaccgct atgtatacct acttgtactt gtagtaagcc   4380

gggttattgg cgttcaatta atcatagact tatgaatctg cacggtgtgc gctgcgagtt   4440

acttttagct tatgcatgct acttgggtgt aatattggga tctgttcgga aatcaacgga   4500

tgctcaatcg atttcgacag taattaatta agtcatacac aagtcagctt tcttcgagcc   4560

tcatataagt ataagtagtt caacgtatta gcactgtacc cagcatctcc gtatcgagaa   4620

acacaacaac atgccccatt ggacagatca tgcggataca caggttgtgc agtatcatac   4680

atactcgatc agacaggtcg tctgaccatc atacaagctg aacaagcgct ccatacttgc   4740

acgctctcta tatacacagt taaattacat atccatagtc taacctctaa cagttaatct   4800

tctggtaagc ctcccagcca gccttctggt atcgcttggc ctcctcaata ggatctcggt   4860

tctggccgta cagacctcgg ccgacaatta tgatatccgt tccggtagac atgacatcct   4920

caacagttcg gtactgctgt ccgagagcgt ctcccttgtc gtcaagaccc accccggggg   4980

tcagaataag ccagtcctca gagtcgccct taggtcggtt ctgggcaatg aagccaacca   5040

caaactcggg gtcggatcgg gcaagctcaa tggtctgctt ggagtactcg ccagtggcca   5100

gagagccctt gcaagacagc tcggccagca tgagcagacc tctggccagc ttctcgttgg   5160

gagaggggac taggaactcc ttgtactggg agttctcgta gtcagagacg tcctccttct   5220

tctgttcaga gacagtttcc tcggcaccag ctcgcaggcc agcaatgatt ccggttccgg   5280

gtacaccgtg ggcgttggtg atatcggacc actcggcgat tcggtgacac cggtactggt   5340

gcttgacagt gttgccaata tctgcgaact ttctgtcctc gaacaggaag aaaccgtgct   5400

taagagcaag ttccttgagg gggagcacag tgccggcgta ggtgaagtcg tcaatgatgt   5460

cgatatgggt tttgatcatg cacacataag gtccgacctt atcggcaagc tcaatgagct   5520

ccttggtggt ggtaacatcc agagaagcac acaggttggt tttcttggct gccacgagct   5580

tgagcactcg agcggcaaag gcggacttgt ggacgttagc tcgagcttcg taggagggca   5640

ttttggtggt gaagaggaga ctgaaataaa tttagtctgc agaacttttt atcggaacct   5700

tatctggggc agtgaagtat atgttatggt aatagttacg agttagttga acttatagat   5760

agactggact atacggctat cggtccaaat tagaaagaac gtcaatggct ctctgggcgt   5820

cgcctttgcc gacaaaaatg tgatcatgat gaaagccagc aatgacgttg cagctgatat   5880

tgttgtcggc caaccgcgcc gaaaacgcag ctgtcagacc cacagcctcc aacgaagaat   5940

gtatcgtcaa agtgatccaa gcacactcat agttggagtc gtactccaaa ggcggcaatg   6000

acgagtcaga cagatactcg tcgacgttta aacagtgtac gcagatctac tatagaggaa   6060

catttaaatt gccccggaga agacggccag gccgcctaga tgacaaattc aacaactcac   6120

agctgacttt ctgccattgc cactaggggg gggccttttt atatggccaa gccaagctct   6180

ccacgtcggt tgggctgcac ccaacaataa atgggtaggg ttgcaccaac aaagggatgg   6240

gatggggggt agaagatacg aggataacgg ggctcaatgg cacaaataag aacgaatact   6300

gccattaaga ctcgtgatcc agcgactgac accattgcat catctaaggg cctcaaaact   6360

acctcggaac tgctgcgctg atctggacac cacagaggtt ccgagcactt taggttgcac   6420

caaatgtccc accaggtgca ggcagaaaac gctggaacag cgtgtacagt ttgtcttagc   6480

aaaaagtgaa ggcgctgagg tcgagcaggg tggtgtgact tgttatagcc tttagagctg   6540

cgaaagcgcg tatggatttg gctcatcagg ccagattgag ggtctgtgga cacatgtcat   6600

gttagtgtac ttcaatcgcc ccctggatat agccccgaca ataggccgtg gcctcatttt   6660

tttgccttcc gcacatttcc attgctcgat acccacacct tgcttctcct gcacttgcca   6720

accttaatac tggtttacat tgaccaacat cttacaagcg gggggcttgt ctagggtata   6780

tataaacagt ggctctccca atcggttgcc agtctctttt ttcctttctt tccccacaga   6840

ttcgaaatct aaactacaca tcacagaatt ccgagccgtg agtatccacg acaagatcag   6900

tgtcgagacg acgcgttttg tgtaatgaca caatccgaaa gtcgctagca acacacactc   6960

tctacacaaa ctaacccagc tctggtacca gc                                 6992


<210>  23
<211>  8707
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  plasmid pY159

<400>  23
ggccgcaagt gtggatgggg aagtgagtgc ccggttctgt gtgcacaatt ggcaatccaa     60

gatggatgga ttcaacacag ggatatagcg agctacgtgg tggtgcgagg atatagcaac    120

ggatatttat gtttgacact tgagaatgta cgatacaagc actgtccaag tacaatacta    180

aacatactgt acatactcat actcgtaccc ggcaacggtt tcacttgagt gcagtggcta    240

gtgctcttac tcgtacagtg tgcaatactg cgtatcatag tctttgatgt atatcgtatt    300

cattcatgtt agttgcgtac gagccggaag cataaagtgt aaagcctggg gtgcctaatg    360

agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct    420

gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg    480

gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc    540

ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg    600

aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct    660

ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca    720

gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct    780

cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc    840

gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt    900

tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc    960

cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc   1020

cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg   1080

gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc   1140

agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag   1200

cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga   1260

tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat   1320

tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag   1380

ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat   1440

cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc   1500

cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat   1560

accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag   1620

ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg   1680

ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc   1740

tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca   1800

acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg   1860

tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc   1920

actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta   1980

ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc   2040

aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg   2100

ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc   2160

cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc   2220

aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat   2280

actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag   2340

cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc   2400

ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt   2460

tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt   2520

cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc   2580

tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga   2640

tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc   2700

cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt   2760

ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct   2820

gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttccattcg   2880

ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc   2940

cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc   3000

cagtcacgac gttgtaaaac gacggccagt gaattgtaat acgactcact atagggcgaa   3060

ttgggtaccg ggccccccct cgaggtcgat ggtgtcgata agcttgatat cgaattcatg   3120

tcacacaaac cgatcttcgc ctcaaggaaa cctaattcta catccgagag actgccgaga   3180

tccagtctac actgattaat tttcgggcca ataatttaaa aaaatcgtgt tatataatat   3240

tatatgtatt atatatatac atcatgatga tactgacagt catgtcccat tgctaaatag   3300

acagactcca tctgccgcct ccaactgatg ttctcaatat ttaaggggtc atctcgcatt   3360

gtttaataat aaacagactc catctaccgc ctccaaatga tgttctcaaa atatattgta   3420

tgaacttatt tttattactt agtattatta gacaacttac ttgctttatg aaaaacactt   3480

cctatttagg aaacaattta taatggcagt tcgttcattt aacaatttat gtagaataaa   3540

tgttataaat gcgtatggga aatcttaaat atggatagca taaatgatat ctgcattgcc   3600

taattcgaaa tcaacagcaa cgaaaaaaat cccttgtaca acataaatag tcatcgagaa   3660

atatcaacta tcaaagaaca gctattcaca cgttactatt gagattatta ttggacgaga   3720

atcacacact caactgtctt tctctcttct agaaatacag gtacaagtat gtactattct   3780

cattgttcat acttctagtc atttcatccc acatattcct tggatttctc tccaatgaat   3840

gacattctat cttgcaaatt caacaattat aataagatat accaaagtag cggtatagtg   3900

gcaatcaaaa agcttctctg gtgtgcttct cgtatttatt tttattctaa tgatccatta   3960

aaggtatata tttatttctt gttatataat ccttttgttt attacatggg ctggatacat   4020

aaaggtattt tgatttaatt ttttgcttaa attcaatccc ccctcgttca gtgtcaactg   4080

taatggtagg aaattaccat acttttgaag aagcaaaaaa aatgaaagaa aaaaaaaatc   4140

gtatttccag gttagacgtt ccgcagaatc tagaatgcgg tatgcggtac attgttcttc   4200

gaacgtaaaa gttgcgctcc ctgagatatt gtacattttt gcttttacaa gtacaagtac   4260

atcgtacaac tatgtactac tgttgatgca tccacaacag tttgttttgt ttttttttgt   4320

tttttttttt tctaatgatt cattaccgct atgtatacct acttgtactt gtagtaagcc   4380

gggttattgg cgttcaatta atcatagact tatgaatctg cacggtgtgc gctgcgagtt   4440

acttttagct tatgcatgct acttgggtgt aatattggga tctgttcgga aatcaacgga   4500

tgctcaatcg atttcgacag taattaatta agtcatacac aagtcagctt tcttcgagcc   4560

tcatataagt ataagtagtt caacgtatta gcactgtacc cagcatctcc gtatcgagaa   4620

acacaacaac atgccccatt ggacagatca tgcggataca caggttgtgc agtatcatac   4680

atactcgatc agacaggtcg tctgaccatc atacaagctg aacaagcgct ccatacttgc   4740

acgctctcta tatacacagt taaattacat atccatagtc taacctctaa cagttaatct   4800

tctggtaagc ctcccagcca gccttctggt atcgcttggc ctcctcaata ggatctcggt   4860

tctggccgta cagacctcgg ccgacaatta tgatatccgt tccggtagac atgacatcct   4920

caacagttcg gtactgctgt ccgagagcgt ctcccttgtc gtcaagaccc accccggggg   4980

tcagaataag ccagtcctca gagtcgccct taggtcggtt ctgggcaatg aagccaacca   5040

caaactcggg gtcggatcgg gcaagctcaa tggtctgctt ggagtactcg ccagtggcca   5100

gagagccctt gcaagacagc tcggccagca tgagcagacc tctggccagc ttctcgttgg   5160

gagaggggac taggaactcc ttgtactggg agttctcgta gtcagagacg tcctccttct   5220

tctgttcaga gacagtttcc tcggcaccag ctcgcaggcc agcaatgatt ccggttccgg   5280

gtacaccgtg ggcgttggtg atatcggacc actcggcgat tcggtgacac cggtactggt   5340

gcttgacagt gttgccaata tctgcgaact ttctgtcctc gaacaggaag aaaccgtgct   5400

taagagcaag ttccttgagg gggagcacag tgccggcgta ggtgaagtcg tcaatgatgt   5460

cgatatgggt tttgatcatg cacacataag gtccgacctt atcggcaagc tcaatgagct   5520

ccttggtggt ggtaacatcc agagaagcac acaggttggt tttcttggct gccacgagct   5580

tgagcactcg agcggcaaag gcggacttgt ggacgttagc tcgagcttcg taggagggca   5640

ttttggtggt gaagaggaga ctgaaataaa tttagtctgc agaacttttt atcggaacct   5700

tatctggggc agtgaagtat atgttatggt aatagttacg agttagttga acttatagat   5760

agactggact atacggctat cggtccaaat tagaaagaac gtcaatggct ctctgggcgt   5820

cgcctttgcc gacaaaaatg tgatcatgat gaaagccagc aatgacgttg cagctgatat   5880

tgttgtcggc caaccgcgcc gaaaacgcag ctgtcagacc cacagcctcc aacgaagaat   5940

gtatcgtcaa agtgatccaa gcacactcat agttggagtc gtactccaaa ggcggcaatg   6000

acgagtcaga cagatactcg tcgacgttta aacagtgtac gcagatctac tatagaggaa   6060

catttaaatt gccccggaga agacggccag gccgcctaga tgacaaattc aacaactcac   6120

agctgacttt ctgccattgc cactaggggg gggccttttt atatggccaa gccaagctct   6180

ccacgtcggt tgggctgcac ccaacaataa atgggtaggg ttgcaccaac aaagggatgg   6240

gatggggggt agaagatacg aggataacgg ggctcaatgg cacaaataag aacgaatact   6300

gccattaaga ctcgtgatcc agcgactgac accattgcat catctaaggg cctcaaaact   6360

acctcggaac tgctgcgctg atctggacac cacagaggtt ccgagcactt taggttgcac   6420

caaatgtccc accaggtgca ggcagaaaac gctggaacag cgtgtacagt ttgtcttagc   6480

aaaaagtgaa ggcgctgagg tcgagcaggg tggtgtgact tgttatagcc tttagagctg   6540

cgaaagcgcg tatggatttg gctcatcagg ccagattgag ggtctgtgga cacatgtcat   6600

gttagtgtac ttcaatcgcc ccctggatat agccccgaca ataggccgtg gcctcatttt   6660

tttgccttcc gcacatttcc attgctcgat acccacacct tgcttctcct gcacttgcca   6720

accttaatac tggtttacat tgaccaacat cttacaagcg gggggcttgt ctagggtata   6780

tataaacagt ggctctccca atcggttgcc agtctctttt ttcctttctt tccccacaga   6840

ttcgaaatct aaactacaca tcacagaatt ccgagccgtg agtatccacg acaagatcag   6900

tgtcgagacg acgcgttttg tgtaatgaca caatccgaaa gtcgctagca acacacactc   6960

tctacacaaa ctaacccagc tctggtacca gcggccatca caagtttgta caaaaaagct   7020

gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa   7080

cagactacat aatactgtaa aacacaacat atccagtcat attggcggcc gcattaggca   7140

ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc   7200

cgtcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca   7260

ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc   7320

aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga   7380

aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc   7440

atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc   7500

cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc   7560

acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa   7620

acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct   7680

gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg   7740

ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc   7800

aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac   7860

agtactgcga tgagtggcag ggcggggcgt aaacgcgtgg atccggctta ctaaaagcca   7920

gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt   7980

atacccgaag tatgtcaaaa agaggtatgc tatgaagcag cgtattacag tgacagttga   8040

cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc   8100

acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag   8160

gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac   8220

aggggctggt gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct   8280

gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct   8340

ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat   8400

cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat   8460

cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct   8520

gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc   8580

atagtgactg gatatgttgt gttttacagc attatgtagt ctgtttttta tgcaaaatct   8640

aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag   8700

tggtgat                                                             8707


<210>  24
<211>  8827
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  plasmid pY169

<400>  24
cttgtacaaa gtggtgatgg ccgcaagtgt ggatggggaa gtgagtgccc ggttctgtgt     60

gcacaattgg caatccaaga tggatggatt caacacaggg atatagcgag ctacgtggtg    120

gtgcgaggat atagcaacgg atatttatgt ttgacacttg agaatgtacg atacaagcac    180

tgtccaagta caatactaaa catactgtac atactcatac tcgtacccgg caacggtttc    240

acttgagtgc agtggctagt gctcttactc gtacagtgtg caatactgcg tatcatagtc    300

tttgatgtat atcgtattca ttcatgttag ttgcgtacga gccggaagca taaagtgtaa    360

agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc    420

tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag    480

aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt    540

cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga    600

atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg    660

taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa    720

aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt    780

tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct    840

gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct    900

cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc    960

cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt   1020

atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc   1080

tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat   1140

ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa   1200

acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa   1260

aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga   1320

aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct   1380

tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga   1440

cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc   1500

catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg   1560

ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat   1620

aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat   1680

ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg   1740

caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc   1800

attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa   1860

agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc   1920

actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt   1980

ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag   2040

ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt   2100

gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag   2160

atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac   2220

cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc   2280

gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca   2340

gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg   2400

ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag   2460

cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc   2520

cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc   2580

tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa   2640

aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg   2700

ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac   2760

actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta   2820

ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac   2880

gcttacaatt tccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg   2940

gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg   3000

gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga attgtaatac   3060

gactcactat agggcgaatt gggtaccggg ccccccctcg aggtcgatgg tgtcgataag   3120

cttgatatcg aattcatgtc acacaaaccg atcttcgcct caaggaaacc taattctaca   3180

tccgagagac tgccgagatc cagtctacac tgattaattt tcgggccaat aatttaaaaa   3240

aatcgtgtta tataatatta tatgtattat atatatacat catgatgata ctgacagtca   3300

tgtcccattg ctaaatagac agactccatc tgccgcctcc aactgatgtt ctcaatattt   3360

aaggggtcat ctcgcattgt ttaataataa acagactcca tctaccgcct ccaaatgatg   3420

ttctcaaaat atattgtatg aacttatttt tattacttag tattattaga caacttactt   3480

gctttatgaa aaacacttcc tatttaggaa acaatttata atggcagttc gttcatttaa   3540

caatttatgt agaataaatg ttataaatgc gtatgggaaa tcttaaatat ggatagcata   3600

aatgatatct gcattgccta attcgaaatc aacagcaacg aaaaaaatcc cttgtacaac   3660

ataaatagtc atcgagaaat atcaactatc aaagaacagc tattcacacg ttactattga   3720

gattattatt ggacgagaat cacacactca actgtctttc tctcttctag aaatacaggt   3780

acaagtatgt actattctca ttgttcatac ttctagtcat ttcatcccac atattccttg   3840

gatttctctc caatgaatga cattctatct tgcaaattca acaattataa taagatatac   3900

caaagtagcg gtatagtggc aatcaaaaag cttctctggt gtgcttctcg tatttatttt   3960

tattctaatg atccattaaa ggtatatatt tatttcttgt tatataatcc ttttgtttat   4020

tacatgggct ggatacataa aggtattttg atttaatttt ttgcttaaat tcaatccccc   4080

ctcgttcagt gtcaactgta atggtaggaa attaccatac ttttgaagaa gcaaaaaaaa   4140

tgaaagaaaa aaaaaatcgt atttccaggt tagacgttcc gcagaatcta gaatgcggta   4200

tgcggtacat tgttcttcga acgtaaaagt tgcgctccct gagatattgt acatttttgc   4260

ttttacaagt acaagtacat cgtacaacta tgtactactg ttgatgcatc cacaacagtt   4320

tgttttgttt ttttttgttt tttttttttc taatgattca ttaccgctat gtatacctac   4380

ttgtacttgt agtaagccgg gttattggcg ttcaattaat catagactta tgaatctgca   4440

cggtgtgcgc tgcgagttac ttttagctta tgcatgctac ttgggtgtaa tattgggatc   4500

tgttcggaaa tcaacggatg ctcaatcgat ttcgacagta attaattaag tcatacacaa   4560

gtcagctttc ttcgagcctc atataagtat aagtagttca acgtattagc actgtaccca   4620

gcatctccgt atcgagaaac acaacaacat gccccattgg acagatcatg cggatacaca   4680

ggttgtgcag tatcatacat actcgatcag acaggtcgtc tgaccatcat acaagctgaa   4740

caagcgctcc atacttgcac gctctctata tacacagtta aattacatat ccatagtcta   4800

acctctaaca gttaatcttc tggtaagcct cccagccagc cttctggtat cgcttggcct   4860

cctcaatagg atctcggttc tggccgtaca gacctcggcc gacaattatg atatccgttc   4920

cggtagacat gacatcctca acagttcggt actgctgtcc gagagcgtct cccttgtcgt   4980

caagacccac cccgggggtc agaataagcc agtcctcaga gtcgccctta ggtcggttct   5040

gggcaatgaa gccaaccaca aactcggggt cggatcgggc aagctcaatg gtctgcttgg   5100

agtactcgcc agtggccaga gagcccttgc aagacagctc ggccagcatg agcagacctc   5160

tggccagctt ctcgttggga gaggggacta ggaactcctt gtactgggag ttctcgtagt   5220

cagagacgtc ctccttcttc tgttcagaga cagtttcctc ggcaccagct cgcaggccag   5280

caatgattcc ggttccgggt acaccgtggg cgttggtgat atcggaccac tcggcgattc   5340

ggtgacaccg gtactggtgc ttgacagtgt tgccaatatc tgcgaacttt ctgtcctcga   5400

acaggaagaa accgtgctta agagcaagtt ccttgagggg gagcacagtg ccggcgtagg   5460

tgaagtcgtc aatgatgtcg atatgggttt tgatcatgca cacataaggt ccgaccttat   5520

cggcaagctc aatgagctcc ttggtggtgg taacatccag agaagcacac aggttggttt   5580

tcttggctgc cacgagcttg agcactcgag cggcaaaggc ggacttgtgg acgttagctc   5640

gagcttcgta ggagggcatt ttggtggtga agaggagact gaaataaatt tagtctgcag   5700

aactttttat cggaacctta tctggggcag tgaagtatat gttatggtaa tagttacgag   5760

ttagttgaac ttatagatag actggactat acggctatcg gtccaaatta gaaagaacgt   5820

caatggctct ctgggcgtcg cctttgccga caaaaatgtg atcatgatga aagccagcaa   5880

tgacgttgca gctgatattg ttgtcggcca accgcgccga aaacgcagct gtcagaccca   5940

cagcctccaa cgaagaatgt atcgtcaaag tgatccaagc acactcatag ttggagtcgt   6000

actccaaagg cggcaatgac gagtcagaca gatactcgtc gacgtttaaa cagtgtacgc   6060

agatctacta tagaggaaca tttaaattgc cccggagaag acggccaggc cgcctagatg   6120

acaaattcaa caactcacag ctgactttct gccattgcca ctaggggggg gcctttttat   6180

atggccaagc caagctctcc acgtcggttg ggctgcaccc aacaataaat gggtagggtt   6240

gcaccaacaa agggatggga tggggggtag aagatacgag gataacgggg ctcaatggca   6300

caaataagaa cgaatactgc cattaagact cgtgatccag cgactgacac cattgcatca   6360

tctaagggcc tcaaaactac ctcggaactg ctgcgctgat ctggacacca cagaggttcc   6420

gagcacttta ggttgcacca aatgtcccac caggtgcagg cagaaaacgc tggaacagcg   6480

tgtacagttt gtcttagcaa aaagtgaagg cgctgaggtc gagcagggtg gtgtgacttg   6540

ttatagcctt tagagctgcg aaagcgcgta tggatttggc tcatcaggcc agattgaggg   6600

tctgtggaca catgtcatgt tagtgtactt caatcgcccc ctggatatag ccccgacaat   6660

aggccgtggc ctcatttttt tgccttccgc acatttccat tgctcgatac ccacaccttg   6720

cttctcctgc acttgccaac cttaatactg gtttacattg accaacatct tacaagcggg   6780

gggcttgtct agggtatata taaacagtgg ctctcccaat cggttgccag tctctttttt   6840

cctttctttc cccacagatt cgaaatctaa actacacatc acagaattcc gagccgtgag   6900

tatccacgac aagatcagtg tcgagacgac gcgttttgtg taatgacaca atccgaaagt   6960

cgctagcaac acacactctc tacacaaact aacccagctc tggtaccagc ggccatcaca   7020

agtttgtaca aaaaagttgg attttttttc gggatggcca ccatctcttt gactactgag   7080

caacttttag aacacccaga actggttgca attgatgggg tgttgtacga tctcttcgga   7140

ctggcgaaag tgcatccagg tggcaacctc attgaagccg ccggtgcctc cgacggaacc   7200

gccctgttct actccatgca ccctggagtg aagccagaga attcgaagct gctgcagcaa   7260

tttgcccgag gcaaacacga acgaagctcg aaggacccag tgtacacctt tgacagtccc   7320

ttcgcccagg atgtcaagca gagcgttcgg gaggtcatga aggggcgcaa ctggtacgcc   7380

acgcccggct tttggctgcg gaccgcgctg atcatcgcgt gcactgccat aggcgaatgg   7440

tattggatca ctaccggggc agtgatgtgg ggcatcttca ccgggtactt ccacagccag   7500

attgggttgg cgattcaaca cgatgcctct cacggagcca tcagcaaaaa gccctgggtg   7560

aacgcctttt tcgcctacgg catcgacgcc attggatcct cccgctggat ctggctgcag   7620

tcccacatta tgcgccacca cacctacacc aaccagcatg gcctggacct ggacgctgcc   7680

tcggcggagc cgttcatttt gttccactcc tacccggcaa caaatgcgtc acgaaagtgg   7740

taccatcggt tccaggcgtg gtacatgtac atcgttttgg ggatgtatgg tgtgtcgatg   7800

gtgtacaatc cgatgtactt gttcacgatg cagcacaacg acacaatccc agaggccacc   7860

tctcttagac caggcagctt tttcaaccgg cagcgcgcct tcgccgtttc cctccgccta   7920

ctgttcatct tccgcaacgc cttcctcccc tggtacatcg cgggcgcctc tccgctgctc   7980

accatcctgc tggtgccaac ggtcacaggc atcttcttga catttgtttt tgtgctgtcc   8040

cataactttg aaggcgctga gcggaccccc gaaaagaact gcaaggccaa aagggccaag   8100

gaggggaagg aggtccgcga tgtagaggag gaccgggtgg actggtaccg ggcgcaggcc   8160

gagaccgcgg cgacctacgg gggcagcgtc gggatgatgc tgaccggcgg tttgaacctg   8220

cagatcgagc accacttgtt cccccgcatg tcctcttggc actacccctt catccaagat   8280

acggtgcggg aatgttgcaa gcgccatggc gtgcgctaca catactaccc gaccatcctg   8340

gagaatataa tgtccacgct ccgctacatg cagaaggtgg gcgtggccca cacaattcag   8400

gatgcccagg aattctgagt gagttcgatc cgcatcgacg tctaccattt ttgatgctgt   8460

ctattcctgt tttcagtcac ctccagcatt ctcatggctg gtgaccactg cccctctaac   8520

ccattgtgac acaccgccaa agactttgcc tctttttttt ccctttcttt tgtcctcggg   8580

gtgctttggc cggtgtttac tcgccttgca gtccccgcaa acgaccgacg tttaagctcc   8640

gttgttgact gggccgctcg taaacccatc tgcaggttga ggctcccatg gagaattgtg   8700

atggctgatt aggaggtggc ggggcataca tgcctcgaca ctcaaagccg ggcggcttct   8760

ggattcgaaa acgcaaatgg gcgctttgga aaaaaaaaaa aaaaaaaaaa aaaaaaaacc   8820

caacttt                                                             8827


<210>  25
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  codon-optimized Euglena anabaena delta-5 desaturase


<220>
<221>  CDS
<222>  (1)..(1362)
<223>  synthetic delta-5 desaturase (codon-optimized) for Yarrowia 
       lipolytica

<400>  25
atg gcc acc atc tcc ctg act acc gag cag ctc ctg gaa cac ccc gag       48
Met Ala Thr Ile Ser Leu Thr Thr Glu Gln Leu Leu Glu His Pro Glu         
1               5                   10                  15              

ctc gtt gcc atc gac gga gtc ctg tac gat ctc ttc ggt ctg gcc aag       96
Leu Val Ala Ile Asp Gly Val Leu Tyr Asp Leu Phe Gly Leu Ala Lys         
            20                  25                  30                  

gtg cat cca gga ggc aac ctc atc gaa gct gcc ggt gca tcc gac gga      144
Val His Pro Gly Gly Asn Leu Ile Glu Ala Ala Gly Ala Ser Asp Gly         
        35                  40                  45                      

acc gct ctg ttc tac tcc atg cat cct gga gtc aag cca gag aac tcg      192
Thr Ala Leu Phe Tyr Ser Met His Pro Gly Val Lys Pro Glu Asn Ser         
    50                  55                  60                          

aag ctt ctg cag caa ttt gcc cga ggc aag cac gaa cga agc tcc aag      240
Lys Leu Leu Gln Gln Phe Ala Arg Gly Lys His Glu Arg Ser Ser Lys         
65                  70                  75                  80          

gat ccc gtg tac acc ttc gac tct ccc ttt gct cag gac gtc aag cag      288
Asp Pro Val Tyr Thr Phe Asp Ser Pro Phe Ala Gln Asp Val Lys Gln         
                85                  90                  95              

tcc gtt cga gag gtc atg aag ggt cga aac tgg tac gcc act cct ggc      336
Ser Val Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly         
            100                 105                 110                 

ttc tgg ctg aga acc gca ctc atc atc gct tgt act gcc att ggc gag      384
Phe Trp Leu Arg Thr Ala Leu Ile Ile Ala Cys Thr Ala Ile Gly Glu         
        115                 120                 125                     

tgg tac tgg atc aca acc gga gca gtg atg tgg ggt atc ttt act gga      432
Trp Tyr Trp Ile Thr Thr Gly Ala Val Met Trp Gly Ile Phe Thr Gly         
    130                 135                 140                         

tac ttc cac tcg cag att ggc ttg gcc att caa cac gat gct tct cac      480
Tyr Phe His Ser Gln Ile Gly Leu Ala Ile Gln His Asp Ala Ser His         
145                 150                 155                 160         

gga gcc atc agc aaa aag ccc tgg gtc aac gcc ttt ttc gct tat ggc      528
Gly Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Phe Phe Ala Tyr Gly         
                165                 170                 175             

atc gac gcc att ggt tcc tct cgt tgg atc tgg ctg cag tcc cac att      576
Ile Asp Ala Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile         
            180                 185                 190                 

atg cga cat cac act tac acc aac cag cat ggc ctc gac ctg gat gct      624
Met Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala         
        195                 200                 205                     

gcc tcg gca gag ccg ttc atc ttg ttc cac tcc tat cct gct acc aac      672
Ala Ser Ala Glu Pro Phe Ile Leu Phe His Ser Tyr Pro Ala Thr Asn         
    210                 215                 220                         

gcc tct cga aag tgg tac cac cga ttt cag gcg tgg tac atg tac atc      720
Ala Ser Arg Lys Trp Tyr His Arg Phe Gln Ala Trp Tyr Met Tyr Ile         
225                 230                 235                 240         

gtt ctg gga atg tat ggt gtc tcg atg gtg tac aat ccc atg tac ctc      768
Val Leu Gly Met Tyr Gly Val Ser Met Val Tyr Asn Pro Met Tyr Leu         
                245                 250                 255             

ttc aca atg cag cac aac gac acc att ccc gag gcc act tct ctc aga      816
Phe Thr Met Gln His Asn Asp Thr Ile Pro Glu Ala Thr Ser Leu Arg         
            260                 265                 270                 

cca ggc agc ttt ttc aat cgg cag cga gct ttc gcc gtt tcc ctt cga      864
Pro Gly Ser Phe Phe Asn Arg Gln Arg Ala Phe Ala Val Ser Leu Arg         
        275                 280                 285                     

ctg ctc ttc atc ttc cga aac gcc ttt ctt ccc tgg tac att gct ggt      912
Leu Leu Phe Ile Phe Arg Asn Ala Phe Leu Pro Trp Tyr Ile Ala Gly         
    290                 295                 300                         

gcc tct cct ctg ctc acc att ctt ctg gtg ccc acg gtc aca ggc atc      960
Ala Ser Pro Leu Leu Thr Ile Leu Leu Val Pro Thr Val Thr Gly Ile         
305                 310                 315                 320         

ttc ctc acc ttt gtg ttc gtt ctg tcc cat aac ttc gag gga gcc gaa     1008
Phe Leu Thr Phe Val Phe Val Leu Ser His Asn Phe Glu Gly Ala Glu         
                325                 330                 335             

cgg acc cca gag aag aac tgc aag gcc aaa cga gct aag gaa ggc aag     1056
Arg Thr Pro Glu Lys Asn Cys Lys Ala Lys Arg Ala Lys Glu Gly Lys         
            340                 345                 350                 

gag gtc aga gac gtg gaa gag gat cga gtc gac tgg tac cga gca cag     1104
Glu Val Arg Asp Val Glu Glu Asp Arg Val Asp Trp Tyr Arg Ala Gln         
        355                 360                 365                     

gcc gag act gct gcc acc tac ggt ggc agc gtg gga atg atg ctt aca     1152
Ala Glu Thr Ala Ala Thr Tyr Gly Gly Ser Val Gly Met Met Leu Thr         
    370                 375                 380                         

ggc ggt ctc aac ctg cag atc gag cat cac ttg ttt ccc cga atg tcc     1200
Gly Gly Leu Asn Leu Gln Ile Glu His His Leu Phe Pro Arg Met Ser         
385                 390                 395                 400         

tct tgg cac tat ccc ttc att caa gac acc gtt cgg gag tgt tgc aag     1248
Ser Trp His Tyr Pro Phe Ile Gln Asp Thr Val Arg Glu Cys Cys Lys         
                405                 410                 415             

cga cat ggc gtc cgt tac aca tac tat cct acc att ctc gag aac atc     1296
Arg His Gly Val Arg Tyr Thr Tyr Tyr Pro Thr Ile Leu Glu Asn Ile         
            420                 425                 430                 

atg tcc act ctt cga tac atg cag aag gtg ggt gtt gct cac acc att     1344
Met Ser Thr Leu Arg Tyr Met Gln Lys Val Gly Val Ala His Thr Ile         
        435                 440                 445                     

cag gat gcc cag gag ttc                                             1362
Gln Asp Ala Gln Glu Phe                                                 
    450                                                                 


<210>  26
<211>  454
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  26

Met Ala Thr Ile Ser Leu Thr Thr Glu Gln Leu Leu Glu His Pro Glu 
1               5                   10                  15      


Leu Val Ala Ile Asp Gly Val Leu Tyr Asp Leu Phe Gly Leu Ala Lys 
            20                  25                  30          


Val His Pro Gly Gly Asn Leu Ile Glu Ala Ala Gly Ala Ser Asp Gly 
        35                  40                  45              


Thr Ala Leu Phe Tyr Ser Met His Pro Gly Val Lys Pro Glu Asn Ser 
    50                  55                  60                  


Lys Leu Leu Gln Gln Phe Ala Arg Gly Lys His Glu Arg Ser Ser Lys 
65                  70                  75                  80  


Asp Pro Val Tyr Thr Phe Asp Ser Pro Phe Ala Gln Asp Val Lys Gln 
                85                  90                  95      


Ser Val Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly 
            100                 105                 110         


Phe Trp Leu Arg Thr Ala Leu Ile Ile Ala Cys Thr Ala Ile Gly Glu 
        115                 120                 125             


Trp Tyr Trp Ile Thr Thr Gly Ala Val Met Trp Gly Ile Phe Thr Gly 
    130                 135                 140                 


Tyr Phe His Ser Gln Ile Gly Leu Ala Ile Gln His Asp Ala Ser His 
145                 150                 155                 160 


Gly Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Phe Phe Ala Tyr Gly 
                165                 170                 175     


Ile Asp Ala Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile 
            180                 185                 190         


Met Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala 
        195                 200                 205             


Ala Ser Ala Glu Pro Phe Ile Leu Phe His Ser Tyr Pro Ala Thr Asn 
    210                 215                 220                 


Ala Ser Arg Lys Trp Tyr His Arg Phe Gln Ala Trp Tyr Met Tyr Ile 
225                 230                 235                 240 


Val Leu Gly Met Tyr Gly Val Ser Met Val Tyr Asn Pro Met Tyr Leu 
                245                 250                 255     


Phe Thr Met Gln His Asn Asp Thr Ile Pro Glu Ala Thr Ser Leu Arg 
            260                 265                 270         


Pro Gly Ser Phe Phe Asn Arg Gln Arg Ala Phe Ala Val Ser Leu Arg 
        275                 280                 285             


Leu Leu Phe Ile Phe Arg Asn Ala Phe Leu Pro Trp Tyr Ile Ala Gly 
    290                 295                 300                 


Ala Ser Pro Leu Leu Thr Ile Leu Leu Val Pro Thr Val Thr Gly Ile 
305                 310                 315                 320 


Phe Leu Thr Phe Val Phe Val Leu Ser His Asn Phe Glu Gly Ala Glu 
                325                 330                 335     


Arg Thr Pro Glu Lys Asn Cys Lys Ala Lys Arg Ala Lys Glu Gly Lys 
            340                 345                 350         


Glu Val Arg Asp Val Glu Glu Asp Arg Val Asp Trp Tyr Arg Ala Gln 
        355                 360                 365             


Ala Glu Thr Ala Ala Thr Tyr Gly Gly Ser Val Gly Met Met Leu Thr 
    370                 375                 380                 


Gly Gly Leu Asn Leu Gln Ile Glu His His Leu Phe Pro Arg Met Ser 
385                 390                 395                 400 


Ser Trp His Tyr Pro Phe Ile Gln Asp Thr Val Arg Glu Cys Cys Lys 
                405                 410                 415     


Arg His Gly Val Arg Tyr Thr Tyr Tyr Pro Thr Ile Leu Glu Asn Ile 
            420                 425                 430         


Met Ser Thr Leu Arg Tyr Met Gln Lys Val Gly Val Ala His Thr Ile 
        435                 440                 445             


Gln Asp Ala Gln Glu Phe 
    450                 


<210>  27
<211>  3983
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  plasmid pEaD5S

<400>  27
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca     60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg    120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc    180

accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc    240

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat    300

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt    360

tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa    420

tgcatctaga tccatggtca agcgacccgc tctgcctctc accgtggacg gtgtcaccta    480

cgacgtttct gcctggctca accaccatcc cggaggtgcc gacattatcg agaactaccg    540

aggtcgggat gctaccgacg tcttcatggt tatgcactcc gagaacgccg tgtccaaact    600

cagacgaatg cccatcatgg aaccttcctc tcccctgact ccaacacctc ccaagccaaa    660

ctccgacgaa cctcaggagg atttccgaaa gctgcgagac gagctcattg ctgcaggcat    720

gttcgatgcc tctcccatgt ggtacgctta caagaccctg tcgactctcg gactgggtgt    780

ccttgccgtg ctgttgatga cccagtggca ctggtacctg gttggtgcta tcgtcctcgg    840

cattcacttt caacagatgg gatggctctc gcacgacatt tgccatcacc agctgttcaa    900

ggaccgatcc atcaacaatg ccattggcct gctcttcgga aacgtgcttc agggcttttc    960

tgtcacttgg tggaaggacc gacacaacgc tcatcactcc gccaccaacg tgcagggtca   1020

cgatcccgac atcgacaacc tgcctctcct ggcgtggtcc aaggaggacg tcgagcgagc   1080

tggcccgttt tctcgacgga tgatcaagta ccaacagtat tacttctttt tcatctgtgc   1140

ccttctgcga ttcatctggt gctttcagtc cattcatact gccacgggtc tcaaggatcg   1200

aagcaatcag tactatcgaa gacagtacga gaaggagtcc gtcggtctgg cactccactg   1260

gggtctcaag gccttgttct actatttcta catgccctcg tttctcaccg gactcatggt   1320

gttctttgtc tccgagctgc ttggtggctt cggaattgcc atcgttgtct tcatgaacca   1380

ctaccctctg gagaagattc aggactccgt gtgggatggt catggcttct gtgctggaca   1440

gattcacgag accatgaacg ttcagcgagg cctcgtcaca gactggtttt tcggtggcct   1500

caactaccag atcgaacatc acctgtggcc tactcttccc agacacaacc tcaccgctgc   1560

ctccatcaaa gtggagcagc tgtgcaagaa gcacaacctg ccctaccgat ctcctcccat   1620

gctcgaaggt gtcggcattc ttatctccta cctgggcacc ttcgctcgaa tggttgccaa   1680

ggcagacaag gcctaagcgg ccgcatcgga tcccgggccc gtcgactgca gaggcctgca   1740

tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac   1800

aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt   1860

gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc   1920

gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg   1980

ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt   2040

atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa   2100

gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc   2160

gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag   2220

gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt   2280

gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg   2340

aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg   2400

ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg   2460

taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac   2520

tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg   2580

gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt   2640

taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg   2700

tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc   2760

tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt   2820

ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt   2880

taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag   2940

tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt   3000

cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc   3060

gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc   3120

cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg   3180

ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac   3240

aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg   3300

atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc   3360

tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact   3420

gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc   3480

aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat   3540

acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc   3600

ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac   3660

tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa   3720

aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact   3780

catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg   3840

atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg   3900

aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag   3960

gcgtatcacg aggccctttc gtc                                           3983


<210>  28
<211>  14688
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZKLeuN-29E3

<400>  28
cgattgttgt ctactaacta tcgtacgata acttcgtata gcatacatta tacgaagtta     60

tcgcgtcgac gagtatctgt ctgactcgtc attgccgcct ttggagtacg actccaacta    120

tgagtgtgct tggatcactt tgacgataca ttcttcgttg gaggctgtgg gtctgacagc    180

tgcgttttcg gcgcggttgg ccgacaacaa tatcagctgc aacgtcattg ctggctttca    240

tcatgatcac atttttgtcg gcaaaggcga cgcccagaga gccattgacg ttctttctaa    300

tttggaccga tagccgtata gtccagtcta tctataagtt caactaactc gtaactatta    360

ccataacata tacttcactg ccccagataa ggttccgata aaaagttctg cagactaaat    420

ttatttcagt ctcctcttca ccaccaaaat gccctcctac gaagctcgag ctaacgtcca    480

caagtccgcc tttgccgctc gagtgctcaa gctcgtggca gccaagaaaa ccaacctgtg    540

tgcttctctg gatgttacca ccaccaagga gctcattgag cttgccgata aggtcggacc    600

ttatgtgtgc atgatcaaaa cccatatcga catcattgac gacttcacct acgccggcac    660

tgtgctcccc ctcaaggaac ttgctcttaa gcacggtttc ttcctgttcg aggacagaaa    720

gttcgcagat attggcaaca ctgtcaagca ccagtaccgg tgtcaccgaa tcgccgagtg    780

gtccgatatc accaacgccc acggtgtacc cggaaccgga atcattgctg gcctgcgagc    840

tggtgccgag gaaactgtct ctgaacagaa gaaggaggac gtctctgact acgagaactc    900

ccagtacaag gagttcctag tcccctctcc caacgagaag ctggccagag gtctgctcat    960

gctggccgag ctgtcttgca agggctctct ggccactggc gagtactcca agcagaccat   1020

tgagcttgcc cgatccgacc ccgagtttgt ggttggcttc attgcccaga accgacctaa   1080

gggcgactct gaggactggc ttattctgac ccccggggtg ggtcttgacg acaagggaga   1140

cgctctcgga cagcagtacc gaactgttga ggatgtcatg tctaccggaa cggatatcat   1200

aattgtcggc cgaggtctgt acggccagaa ccgagatcct attgaggagg ccaagcgata   1260

ccagaaggct ggctgggagg cttaccagaa gattaactgt tagaggttag actatggata   1320

tgtaatttaa ctgtgtatat agagagcgtg caagtatgga gcgcttgttc agcttgtatg   1380

atggtcagac gacctgtctg atcgagtatg tatgatactg cacaacctgt gtatccgcat   1440

gatctgtcca atggggcatg ttgttgtgtt tctcgatacg gagatgctgg gtacagtgct   1500

aatacgttga actacttata cttatatgag gctcgaagaa agctgacttg tgtatgactt   1560

attctcaact acatccccag tcacaatacc accactgcac taccactaca ccaaaaccat   1620

gatcaaacca cccatggact tcctggaggc agaagaactt gttatggaaa agctcaagag   1680

agagatcata acttcgtata gcatacatta tacgaagtta tcctgcaggt aaaggaattc   1740

tggagtttct gagagaaaaa ggcaagatac gtatgtaaca aagcgacgca tggtacaata   1800

ataccggagg catgtatcat agagagttag tggttcgatg atggcactgg tgcctggtat   1860

gactttatac ggctgactac atatttgtcc tcagacatac aattacagtc aagcacttac   1920

ccttggacat ctgtaggtac cccccggcca agacgatctc agcgtgtcgt atgtcggatt   1980

ggcgtagctc cctcgctcgt caattggctc ccatctactt tcttctgctt ggctacaccc   2040

agcatgtctg ctatggctcg ttttcgtgcc ttatctatcc tcccagtatt accaactcta   2100

aatgacatga tgtgattggg tctacacttt catatcagag ataaggagta gcacagttgc   2160

ataaaaagcc caactctaat cagcttcttc ctttcttgta attagtacaa aggtgattag   2220

cgaaatctgg aagcttagtt ggccctaaaa aaatcaaaaa aagcaaaaaa cgaaaaacga   2280

aaaaccacag ttttgagaac agggaggtaa cgaaggatcg tatatatata tatatatata   2340

tatacccacg gatcccgaga ccggcctttg attcttccct acaaccaacc attctcacca   2400

ccctaattca caaccatgga gtctggaccc atgcctgctg gcattccctt ccctgagtac   2460

tatgacttct ttatggactg gaagactccc ctggccatcg ctgccaccta cactgctgcc   2520

gtcggtctct tcaaccccaa ggttggcaag gtctcccgag tggttgccaa gtcggctaac   2580

gcaaagcctg ccgagcgaac ccagtccgga gctgccatga ctgccttcgt ctttgtgcac   2640

aacctcattc tgtgtgtcta ctctggcatc accttctact acatgtttcc tgctatggtc   2700

aagaacttcc gaacccacac actgcacgaa gcctactgcg acacggatca gtccctctgg   2760

aacaacgcac ttggctactg gggttacctc ttctacctgt ccaagttcta cgaggtcatt   2820

gacaccatca tcatcatcct gaagggacga cggtcctcgc tgcttcagac ctaccaccat   2880

gctggagcca tgattaccat gtggtctggc atcaactacc aagccactcc catttggatc   2940

tttgtggtct tcaactcctt cattcacacc atcatgtact gttactatgc cttcacctct   3000

atcggattcc atcctcctgg caaaaagtac ctgacttcga tgcagattac tcagtttctg   3060

gtcggtatca ccattgccgt gtcctacctc ttcgttcctg gctgcatccg aacacccggt   3120

gctcagatgg ctgtctggat caacgtcggc tacctgtttc ccttgaccta tctgttcgtg   3180

gactttgcca agcgaaccta ctccaagcga tctgccattg ccgctcagaa aaaggctcag   3240

taagcggccg cattgatgat tggaaacaca cacatgggtt atatctaggt gagagttagt   3300

tggacagtta tatattaaat cagctatgcc aacggtaact tcattcatgt caacgaggaa   3360

ccagtgactg caagtaatat agaatttgac caccttgcca ttctcttgca ctcctttact   3420

atatctcatt tatttcttat atacaaatca cttcttcttc ccagcatcga gctcggaaac   3480

ctcatgagca ataacatcgt ggatctcgtc aatagagggc tttttggact ccttgctgtt   3540

ggccaccttg tccttgctgt ctggctcatt ctgtttcaac gccttttaat taacggagta   3600

ggtctcggtg tcggaagcga cgccagatcc gtcatcctcc tttcgctctc caaagtagat   3660

acctccgacg agctctcgga caatgatgaa gtcggtgccc tcaacgtttc ggatggggga   3720

gagatcggcg agcttgggcg acagcagctg gcagggtcgc aggttggcgt acaggttcag   3780

gtcctttcgc agcttgagga gaccctgctc gggtcgcacg tcggttcgtc cgtcgggagt   3840

ggtccatacg gtgttggcag cgcctccgac agcaccgagc ataatagagt cagcctttcg   3900

gcagatgtcg agagtagcgt cggtgatggg ctcgccctcc ttctcaatgg cagctcctcc   3960

aatgagtcgg tcctcaaaca caaactcggt gccggaggcc tcagcaacag acttgagcac   4020

cttgacggcc tcggcaatca cctcggggcc acagaagtcg ccgccgagaa gaacaatctt   4080

cttggagtca gtcttggtct tcttagtttc gggttccatt gtggatgtgt gtggttgtat   4140

gtgtgatgtg gtgtgtggag tgaaaatctg tggctggcaa acgctcttgt atatatacgc   4200

acttttgccc gtgctatgtg gaagactaaa cctccgaaga ttgtgactca ggtagtgcgg   4260

tatcggctag ggacccaaac cttgtcgatg ccgatagcat gcgacgtcgg gcccaattcg   4320

ccctatagtg agtcgtatta caattcactg gccgtcgttt tacaacgtcg tgactgggaa   4380

aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt   4440

aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa   4500

tggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga   4560

ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg   4620

ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat   4680

ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg   4740

ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata   4800

gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt   4860

tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat   4920

ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc ctgatgcggt attttctcct   4980

tacgcatctg tgcggtattt cacaccgcat caggtggcac ttttcgggga aatgtgcgcg   5040

gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat   5100

aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc   5160

gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa   5220

cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac   5280

tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga   5340

tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag   5400

agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca   5460

cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca   5520

tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa   5580

ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc   5640

tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa   5700

cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag   5760

actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct   5820

ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac   5880

tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa   5940

ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt   6000

aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat   6060

ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg   6120

agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc   6180

ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg   6240

tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag   6300

cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact   6360

ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg   6420

gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc   6480

ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg   6540

aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg   6600

cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag   6660

ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc   6720

gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct   6780

ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc   6840

ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc   6900

gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac   6960

cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcgcgcccac tgagctcgtc   7020

taacggactt gatatacaac caattaaaac aaatgaaaag aaatacagtt ctttgtatca   7080

tttgtaacaa ttaccctgta caaactaagg tattgaaatc ccacaatatt cccaaagtcc   7140

acccctttcc aaattgtcat gcctacaact catataccaa gcactaacct accaaacacc   7200

actaaaaccc cacaaaatat atcttaccga atatacagta acaagctacc accacactcg   7260

ttgggtgcag tcgccagctt aaagatatct atccacatca gccacaactc ccttccttta   7320

ataaaccgac tacacccttg gctattgagg ttatgagtga atatactgta gacaagacac   7380

tttcaagaag actgtttcca aaacgtacca ctgtcctcca ctacaaacac acccaatctg   7440

cttcttctag tcaaggttgc tacaccggta aattataaat catcatttca ttagcagggc   7500

agggcccttt ttatagagtc ttatacacta gcggaccctg ccggtagacc aacccgcagg   7560

cgcgtcagtt tgctccttcc atcaatgcgt cgtagaaacg acttactcct tcttgagcag   7620

ctccttgacc ttgttggcaa caagtctccg acctcggagg tggaggaaga gcctccgata   7680

tcggcggtag tgataccagc ctcgacggac tccttgacgg cagcctcaac agcgtcaccg   7740

gcgggcttca tgttaagaga gaacttgagc atcatggcgg cagacagaat ggtggcgtac   7800

gcaactaaca tgaatgaata cgatatacat caaagactat gatacgcagt attgcacact   7860

gtacgagtaa gagcactagc cactgcactc aagtgaaacc gttgcccggg tacgagtatg   7920

agtatgtaca gtatgtttag tattgtactt ggacagtgct tgtatcgtac attctcaagt   7980

gtcaaacata aatatccgtt gctatatcct cgcaccacca cgtagctcgc tatatccctg   8040

tgttgaatcc atccatcttg gattgccaat tgtgcacaca gaaccgggca ctcacttccc   8100

catccacact tgcggccgct taagcaacgg gcttgataac agcggggggg gtgcccacgt   8160

tgttgcggtt gcggaagaac agaacaccct taccagcacc ctcggcacca gcgctgggct   8220

caacccactg gcacatacgc gcactgcggt acatggcgcg gatgaagcca cgaggaccat   8280

cctggacatc agcccggtag tgcttgccca tgatgggctt aatggcctcg gtggcctcgt   8340

ccgcgttgta gaaggggatg ctgctgacgt agtggtggag gacatgagtc tcgatgatgc   8400

cgtggagaag gtggcggccg atgaagccca tctcacggtc aatggtagca gcggcaccac   8460

ggacgaagtt ccactcgtcg ttggtgtagt ggggaagggt agggtcggtg tgctggagga   8520

aggtgatggc aacgagccag tggttaaccc agaggtaggg aacaaagtac cagatggcca   8580

tgttgtagaa accgaacttc tgaacgagga agtacagagc agtggccatc agaccgatac   8640

caatatcgct gaggacgatg agcttagcgt cactgttctc gtacagaggg ctgcggggat   8700

cgaagtggtt aacaccaccg ccgaggccgt tatgcttgcc cttgccgcga ccctcacgct   8760

ggcgctcgtg gtagttgtgg ccggtaacat tggtgatgag gtagttgggc cagccaacga   8820

gctgctgaag gacgagcatg agaagagtga aagcgggggt ctcctcagta agatgagcga   8880

gctcgtgggt catctttccg agacgagtag cctgctgctc gcgggttcgg ggaacgaaga   8940

ccatgtcacg ctccatgttg ccagtggcct tgtggtgctt tcggtgggag atttgccagc   9000

tgaagtaggg gacaaggagg gaagagtgaa gaacccagcc agtaatgtcg ttgatgatgc   9060

gagaatcgga gaaagcaccg tgaccgcact catgggcaat aacccagaga ccagtaccga   9120

aaagaccctg aagaacggtg tacacggccc acagaccagc gcgggcgggg gtggagggga   9180

tatattcggg ggtcacaaag ttgtaccaga tgctgaaagt ggtagtcagg aggacaatgt   9240

cgcggaggat ataaccgtat cccttgagag cggagcgctt gaagcagtgc ttagggatgg   9300

cattgtagat gtccttgatg gtaaagtcgg gaacctcgaa ctggttgccg taggtgtcga   9360

gcatgacacc atactcggac ttgggcttgg cgatatcaac ctcggacatg gacgagagcg   9420

atgtggaaga ggccgagtgg cggggagagt ctgaaggaga gacggcggca gactcagaat   9480

ccgtcacagt agttgaggtg acggtgcgtc taagcgcagg gttctgcttg ggcagagccg   9540

aagtggacgc catggttgat gtgtgtttaa ttcaagaatg aatatagaga agagaagaag   9600

aaaaaagatt caattgagcc ggcgatgcag acccttatat aaatgttgcc ttggacagac   9660

ggagcaagcc cgcccaaacc tacgttcggt ataatatgtt aagcttttta acacaaaggt   9720

ttggcttggg gtaacctgat gtggtgcaaa agaccgggcg ttggcgagcc attgcgcggg   9780

cgaatggggc cgtgactcgt ctcaaattcg agggcgtgcc tcaattcgtg cccccgtggc   9840

tttttcccgc cgtttccgcc ccgtttgcac cactgcagcc gcttctttgg ttcggacacc   9900

ttgctgcgag ctaggtgcct tgtgctactt aaaaagtggc ctcccaacac caacatgaca   9960

tgagtgcgtg ggccaagaca cgttggcggg gtcgcagtcg gctcaatggc ccggaaaaaa  10020

cgctgctgga gctggttcgg acgcagtccg ccgcggcgta tggatatccg caaggttcca  10080

tagcgccatt gccctccgtc ggcgtctatc ccgcaacctc taaatagagc gggaatataa  10140

cccaagcttc ttttttttcc tttaacacgc acacccccaa ctatcatgtt gctgctgctg  10200

tttgactcta ctctgtggag gggtgctccc acccaaccca acctacaggt ggatccggcg  10260

ctgtgattgg ctgataagtc tcctatccgg actaattctg accaatggga catgcgcgca  10320

ggacccaaat gccgcaatta cgtaacccca acgaaatgcc tacccctctt tggagcccag  10380

cggccccaaa tccccccaag cagcccggtt ctaccggctt ccatctccaa gcacaagcag  10440

cccggttcta ccggcttcca tctccaagca cccctttctc cacaccccac aaaaagaccc  10500

gtgcaggaca tcctactgcg tcgacatcat ttaaattcct tcacttcaag ttcattcttc  10560

atctgcttct gttttacttt gacaggcaaa tgaagacatg gtacgacttg atggaggcca  10620

agaacgccat ttcaccccga gacaccgaag tgcctgaaat cctggctgcc cccattgata  10680

acatcggaaa ctacggtatt ccggaaagtg tatatagaac ctttccccag cttgtgtctg  10740

tggatatgga tggtgtaatc ccctttgagt actcgtcttg gcttctctcc gagcagtatg  10800

aggctctcta atctagcgca tttaatatct caatgtattt atatatttat cttctcatgc  10860

ggccgctcac tgaatctttt tggctccctt gtgcttcctg acgatatacg tttgcacata  10920

gaaattcaag aacaaacaca agactgtgcc aacataaaag taattgaaga accagccaaa  10980

catcctcatc ccatcttggc gataacaggg aatgttcctg tacttccaga caatgtagaa  11040

accaacattg aattgaatga tctgcattga tgtaatcagg gattttggca tggggaactt  11100

cagcttgatc aatctggtcc aataataacc gtacatgatc cagtggatga aaccattcaa  11160

cagcacaaaa atccaaacag cttcatttcg gtaattatag aacagccaca tatccatcgg  11220

tgcccccaaa tgatggaaga attgcaacca ggtcagaggc ttgcccatca gtggcaaata  11280

gaaggagtca atatactcca ggaacttgct caaatagaac aactgcgtgg tgatcctgaa  11340

gacgttgttg tcaaaagcct tctcgcagtt gtcagacata acaccgatgg tgtacatggc  11400

atatgccatt gagaggaatg atcccaacga ataaatggac atgagaaggt tgtaattggt  11460

gaaaacaaac ttcatacgag actgaccttt tggaccaagg gggccaagag tgaacttcaa  11520

gatgacaaat gcgatggaca agtaaagcac ctcacagtga ctggcatcac tccagagttg  11580

ggcataatca actggttggg taaaacttcc tgcccaattg agactatttc attcaccacc  11640

tccatggcca ttgctgtaga tatgtcttgt gtgtaagggg gttggggtgg ttgtttgtgt  11700

tcttgacttt tgtgttagca agggaagacg ggcaaaaaag tgagtgtggt tgggagggag  11760

agacgagcct tatatataat gcttgtttgt gtttgtgcaa gtggacgccg aaacgggcag  11820

gagccaaact aaacaaggca gacaatgcga gcttaattgg attgcctgat gggcaggggt  11880

tagggctcga tcaatggggg tgcgaagtga caaaattggg aattaggttc gcaagcaagg  11940

ctgacaagac tttggcccaa acatttgtac gcggtggaca acaggagcca cccatcgtct  12000

gtcacgggct agccggtcgt gcgtcctgtc aggctccacc taggctccat gccactccat  12060

acaatcccac tagtgtaccg ctaggccgct tttagctccc atctaagacc cccccaaaac  12120

ctccactgta cagtgcactg tactgtgtgg cgatcaaggg caagggaaaa aaggcgcaaa  12180

catgcacgca tggaatgacg taggtaaggc gttactagac tgaaaagtgg cacatttcgg  12240

cgtgccaaag ggtcctaggt gcgtttcgcg agctgggcgc caggccaagc cgctccaaaa  12300

cgcctctccg actccctcca gcggcctcca tatccccatc cctctccaca gcaatgttgt  12360

taagccttgc aaacgaaaaa atagaaaggc taataagctt ccaatattgt ggtgtacgct  12420

gcataacgca acaatgagcg ccaaacaaca cacacacaca gcacacagca gcattaacca  12480

cgatgaacag catgacatta caggtgggtg tgtaatcagg gccctgattg ctggtggtgg  12540

gagcccccat catgggcaga tctgcgtaca ctgtttaaac agtgtacgca gatctactat  12600

agaggaacat ttaaattgcc ccggagaaga cggccaggcc gcctagatga caaattcaac  12660

aactcacagc tgactttctg ccattgccac tagggggggg cctttttata tggccaagcc  12720

aagctctcca cgtcggttgg gctgcaccca acaataaatg ggtagggttg caccaacaaa  12780

gggatgggat ggggggtaga agatacgagg ataacggggc tcaatggcac aaataagaac  12840

gaatactgcc attaagactc gtgatccagc gactgacacc attgcatcat ctaagggcct  12900

caaaactacc tcggaactgc tgcgctgatc tggacaccac agaggttccg agcactttag  12960

gttgcaccaa atgtcccacc aggtgcaggc agaaaacgct ggaacagcgt gtacagtttg  13020

tcttaacaaa aagtgagggc gctgaggtcg agcagggtgg tgtgacttgt tatagccttt  13080

agagctgcga aagcgcgtat ggatttggct catcaggcca gattgagggt ctgtggacac  13140

atgtcatgtt agtgtacttc aatcgccccc tggatatagc cccgacaata ggccgtggcc  13200

tcattttttt gccttccgca catttccatt gctcgatacc cacaccttgc ttctcctgca  13260

cttgccaacc ttaatactgg tttacattga ccaacatctt acaagcgggg ggcttgtcta  13320

gggtatatat aaacagtggc tctcccaatc ggttgccagt ctcttttttc ctttctttcc  13380

ccacagattc gaaatctaaa ctacacatca cagaattccg agccgtgagt atccacgaca  13440

agatcagtgt cgagacgacg cgttttgtgt aatgacacaa tccgaaagtc gctagcaaca  13500

cacactctct acacaaacta acccagctct ggtaccatgg aggtcgtgaa cgaaatcgtc  13560

tccattggcc aggaggttct tcccaaggtc gactatgctc agctctggtc tgatgcctcg  13620

cactgcgagg tgctgtacct ctccatcgcc ttcgtcatcc tgaagttcac ccttggtcct  13680

ctcggaccca agggtcagtc tcgaatgaag tttgtgttca ccaactacaa cctgctcatg  13740

tccatctact cgctgggctc cttcctctct atggcctacg ccatgtacac cattggtgtc  13800

atgtccgaca actgcgagaa ggctttcgac aacaatgtct tccgaatcac cactcagctg  13860

ttctacctca gcaagttcct cgagtacatt gactccttct atctgcccct catgggcaag  13920

cctctgacct ggttgcagtt ctttcaccat ctcggagctc ctatggacat gtggctgttc  13980

tacaactacc gaaacgaagc cgtttggatc tttgtgctgc tcaacggctt cattcactgg  14040

atcatgtacg gctactattg gacccgactg atcaagctca agttccctat gcccaagtcc  14100

ctgattactt ctatgcagat cattcagttc aacgttggct tctacatcgt ctggaagtac  14160

cggaacattc cctgctaccg acaagatgga atgagaatgt ttggctggtt tttcaactac  14220

ttctacgttg gtactgtcct gtgtctgttc ctcaacttct acgtgcagac ctacatcgtc  14280

cgaaagcaca agggagccaa aaagattcag tgagcggccg catgtacata caagattatt  14340

tatagaaatg aatcgcgatc gaacaaagag tacgagtgta cgagtagggg atgatgataa  14400

aagtggaaga agttccgcat ctttggattt atcaacgtgt aggacgatac ttcctgtaaa  14460

aatgcaatgt ctttaccata ggttctgctg tagatgttat taactaccat taacatgtct  14520

acttgtacag ttgcagacca gttggagtat agaatggtac acttaccaaa aagtgttgat  14580

ggttgtaact acgatatata aaactgttga cgggatcccc gctgatatgc ctaaggaaca  14640

atcaaagagg aagatattaa ttcagaatgc tagtatacag ttagggat               14688


<210>  29
<211>  1434
<212>  DNA
<213>  Fusarium monoliforme


<220>
<221>  CDS
<222>  (1)..(1434)
<223>  delta-12 desaturase

<300>
<302>  DELTA-12 DESATURASES SUITABLE FOR ALTERING LEVELS OF 
       POLYUNSATURATED FATTY ACIDS IN OLEAGINOUS YEAST
<310>  WO 2005/047485
<311>  2004-11-12
<312>  2005-05-26
<313>  (1)..(1434)

<300>
<302>  DELTA-12 DESATURASES SUITABLE FOR ALTERING LEVELS OF 
       POLYUNSATURATED FATTY ACIDS IN OLEAGINOUS YEAST
<310>  US 2005-0216975-A1
<311>  2004-11-10
<312>  2005-09-29
<313>  (1)..(1434)

<400>  29
atg gcg tcc act tcg gct ctg ccc aag cag aac cct gcg ctt aga cgc       48
Met Ala Ser Thr Ser Ala Leu Pro Lys Gln Asn Pro Ala Leu Arg Arg         
1               5                   10                  15              

acc gtc acc tca act act gtg acg gat tct gag tct gcc gcc gtc tct       96
Thr Val Thr Ser Thr Thr Val Thr Asp Ser Glu Ser Ala Ala Val Ser         
            20                  25                  30                  

cct tca gac tct ccc cgc cac tcg gcc tct tcc aca tcg ctc tcg tcc      144
Pro Ser Asp Ser Pro Arg His Ser Ala Ser Ser Thr Ser Leu Ser Ser         
        35                  40                  45                      

atg tcc gag gtt gat atc gcc aag ccc aag tcc gag tat ggt gtc atg      192
Met Ser Glu Val Asp Ile Ala Lys Pro Lys Ser Glu Tyr Gly Val Met         
    50                  55                  60                          

ctc gac acc tac ggc aac cag ttc gag gtt ccc gac ttt acc atc aag      240
Leu Asp Thr Tyr Gly Asn Gln Phe Glu Val Pro Asp Phe Thr Ile Lys         
65                  70                  75                  80          

gac atc tac aat gcc atc cct aag cac tgc ttc aag cgc tcc gct ctc      288
Asp Ile Tyr Asn Ala Ile Pro Lys His Cys Phe Lys Arg Ser Ala Leu         
                85                  90                  95              

aag gga tac ggt tat atc ctc cgc gac att gtc ctc ctg act acc act      336
Lys Gly Tyr Gly Tyr Ile Leu Arg Asp Ile Val Leu Leu Thr Thr Thr         
            100                 105                 110                 

ttc agc atc tgg tac aac ttt gtg acc ccc gaa tat atc ccc tcc acc      384
Phe Ser Ile Trp Tyr Asn Phe Val Thr Pro Glu Tyr Ile Pro Ser Thr         
        115                 120                 125                     

ccc gcc cgc gct ggt ctg tgg gcc gtg tac acc gtt ctt cag ggt ctt      432
Pro Ala Arg Ala Gly Leu Trp Ala Val Tyr Thr Val Leu Gln Gly Leu         
    130                 135                 140                         

ttc ggt act ggt ctc tgg gtt att gcc cat gag tgc ggt cac ggt gct      480
Phe Gly Thr Gly Leu Trp Val Ile Ala His Glu Cys Gly His Gly Ala         
145                 150                 155                 160         

ttc tcc gat tct cgc atc atc aac gac att act ggc tgg gtt ctt cac      528
Phe Ser Asp Ser Arg Ile Ile Asn Asp Ile Thr Gly Trp Val Leu His         
                165                 170                 175             

tct tcc ctc ctt gtc ccc tac ttc agc tgg caa atc tcc cac cga aag      576
Ser Ser Leu Leu Val Pro Tyr Phe Ser Trp Gln Ile Ser His Arg Lys         
            180                 185                 190                 

cac cac aag gcc act ggc aac atg gag cgt gac atg gtc ttc gtt ccc      624
His His Lys Ala Thr Gly Asn Met Glu Arg Asp Met Val Phe Val Pro         
        195                 200                 205                     

cga acc cgc gag cag cag gct act cgt ctc gga aag atg acc cac gag      672
Arg Thr Arg Glu Gln Gln Ala Thr Arg Leu Gly Lys Met Thr His Glu         
    210                 215                 220                         

ctc gct cat ctt act gag gag acc ccc gct ttc act ctt ctc atg ctc      720
Leu Ala His Leu Thr Glu Glu Thr Pro Ala Phe Thr Leu Leu Met Leu         
225                 230                 235                 240         

gtc ctt cag cag ctc gtt ggc tgg ccc aac tac ctc atc acc aat gtt      768
Val Leu Gln Gln Leu Val Gly Trp Pro Asn Tyr Leu Ile Thr Asn Val         
                245                 250                 255             

acc ggc cac aac tac cac gag cgc cag cgt gag ggt cgc ggc aag ggc      816
Thr Gly His Asn Tyr His Glu Arg Gln Arg Glu Gly Arg Gly Lys Gly         
            260                 265                 270                 

aag cat aac ggc ctc ggc ggt ggt gtt aac cac ttc gat ccc cgc agc      864
Lys His Asn Gly Leu Gly Gly Gly Val Asn His Phe Asp Pro Arg Ser         
        275                 280                 285                     

cct ctg tac gag aac agt gac gct aag ctc atc gtc ctc agc gat att      912
Pro Leu Tyr Glu Asn Ser Asp Ala Lys Leu Ile Val Leu Ser Asp Ile         
    290                 295                 300                         

ggt atc ggt ctg atg gcc act gct ctg tac ttc ctc gtt cag aag ttc      960
Gly Ile Gly Leu Met Ala Thr Ala Leu Tyr Phe Leu Val Gln Lys Phe         
305                 310                 315                 320         

ggt ttc tac aac atg gcc atc tgg tac ttt gtt ccc tac ctc tgg gtt     1008
Gly Phe Tyr Asn Met Ala Ile Trp Tyr Phe Val Pro Tyr Leu Trp Val         
                325                 330                 335             

aac cac tgg ctc gtt gcc atc acc ttc ctc cag cac acc gac cct acc     1056
Asn His Trp Leu Val Ala Ile Thr Phe Leu Gln His Thr Asp Pro Thr         
            340                 345                 350                 

ctt ccc cac tac acc aac gac gag tgg aac ttc gtc cgt ggt gcc gct     1104
Leu Pro His Tyr Thr Asn Asp Glu Trp Asn Phe Val Arg Gly Ala Ala         
        355                 360                 365                     

gct acc att gac cgt gag atg ggc ttc atc ggc cgc cac ctt ctc cac     1152
Ala Thr Ile Asp Arg Glu Met Gly Phe Ile Gly Arg His Leu Leu His         
    370                 375                 380                         

ggc atc atc gag act cat gtc ctc cac cac tac gtc agc agc atc ccc     1200
Gly Ile Ile Glu Thr His Val Leu His His Tyr Val Ser Ser Ile Pro         
385                 390                 395                 400         

ttc tac aac gcg gac gag gcc acc gag gcc att aag ccc atc atg ggc     1248
Phe Tyr Asn Ala Asp Glu Ala Thr Glu Ala Ile Lys Pro Ile Met Gly         
                405                 410                 415             

aag cac tac cgg gct gat gtc cag gat ggt cct cgt ggc ttc atc cgc     1296
Lys His Tyr Arg Ala Asp Val Gln Asp Gly Pro Arg Gly Phe Ile Arg         
            420                 425                 430                 

gcc atg tac cgc agt gcg cgt atg tgc cag tgg gtt gag ccc agc gct     1344
Ala Met Tyr Arg Ser Ala Arg Met Cys Gln Trp Val Glu Pro Ser Ala         
        435                 440                 445                     

ggt gcc gag ggt gct ggt aag ggt gtt ctg ttc ttc cgc aac cgc aac     1392
Gly Ala Glu Gly Ala Gly Lys Gly Val Leu Phe Phe Arg Asn Arg Asn         
    450                 455                 460                         

aac gtg ggc acc ccc ccc gct gtt atc aag ccc gtt gct taa             1434
Asn Val Gly Thr Pro Pro Ala Val Ile Lys Pro Val Ala                     
465                 470                 475                             


<210>  30
<211>  477
<212>  PRT
<213>  Fusarium monoliforme

<400>  30

Met Ala Ser Thr Ser Ala Leu Pro Lys Gln Asn Pro Ala Leu Arg Arg 
1               5                   10                  15      


Thr Val Thr Ser Thr Thr Val Thr Asp Ser Glu Ser Ala Ala Val Ser 
            20                  25                  30          


Pro Ser Asp Ser Pro Arg His Ser Ala Ser Ser Thr Ser Leu Ser Ser 
        35                  40                  45              


Met Ser Glu Val Asp Ile Ala Lys Pro Lys Ser Glu Tyr Gly Val Met 
    50                  55                  60                  


Leu Asp Thr Tyr Gly Asn Gln Phe Glu Val Pro Asp Phe Thr Ile Lys 
65                  70                  75                  80  


Asp Ile Tyr Asn Ala Ile Pro Lys His Cys Phe Lys Arg Ser Ala Leu 
                85                  90                  95      


Lys Gly Tyr Gly Tyr Ile Leu Arg Asp Ile Val Leu Leu Thr Thr Thr 
            100                 105                 110         


Phe Ser Ile Trp Tyr Asn Phe Val Thr Pro Glu Tyr Ile Pro Ser Thr 
        115                 120                 125             


Pro Ala Arg Ala Gly Leu Trp Ala Val Tyr Thr Val Leu Gln Gly Leu 
    130                 135                 140                 


Phe Gly Thr Gly Leu Trp Val Ile Ala His Glu Cys Gly His Gly Ala 
145                 150                 155                 160 


Phe Ser Asp Ser Arg Ile Ile Asn Asp Ile Thr Gly Trp Val Leu His 
                165                 170                 175     


Ser Ser Leu Leu Val Pro Tyr Phe Ser Trp Gln Ile Ser His Arg Lys 
            180                 185                 190         


His His Lys Ala Thr Gly Asn Met Glu Arg Asp Met Val Phe Val Pro 
        195                 200                 205             


Arg Thr Arg Glu Gln Gln Ala Thr Arg Leu Gly Lys Met Thr His Glu 
    210                 215                 220                 


Leu Ala His Leu Thr Glu Glu Thr Pro Ala Phe Thr Leu Leu Met Leu 
225                 230                 235                 240 


Val Leu Gln Gln Leu Val Gly Trp Pro Asn Tyr Leu Ile Thr Asn Val 
                245                 250                 255     


Thr Gly His Asn Tyr His Glu Arg Gln Arg Glu Gly Arg Gly Lys Gly 
            260                 265                 270         


Lys His Asn Gly Leu Gly Gly Gly Val Asn His Phe Asp Pro Arg Ser 
        275                 280                 285             


Pro Leu Tyr Glu Asn Ser Asp Ala Lys Leu Ile Val Leu Ser Asp Ile 
    290                 295                 300                 


Gly Ile Gly Leu Met Ala Thr Ala Leu Tyr Phe Leu Val Gln Lys Phe 
305                 310                 315                 320 


Gly Phe Tyr Asn Met Ala Ile Trp Tyr Phe Val Pro Tyr Leu Trp Val 
                325                 330                 335     


Asn His Trp Leu Val Ala Ile Thr Phe Leu Gln His Thr Asp Pro Thr 
            340                 345                 350         


Leu Pro His Tyr Thr Asn Asp Glu Trp Asn Phe Val Arg Gly Ala Ala 
        355                 360                 365             


Ala Thr Ile Asp Arg Glu Met Gly Phe Ile Gly Arg His Leu Leu His 
    370                 375                 380                 


Gly Ile Ile Glu Thr His Val Leu His His Tyr Val Ser Ser Ile Pro 
385                 390                 395                 400 


Phe Tyr Asn Ala Asp Glu Ala Thr Glu Ala Ile Lys Pro Ile Met Gly 
                405                 410                 415     


Lys His Tyr Arg Ala Asp Val Gln Asp Gly Pro Arg Gly Phe Ile Arg 
            420                 425                 430         


Ala Met Tyr Arg Ser Ala Arg Met Cys Gln Trp Val Glu Pro Ser Ala 
        435                 440                 445             


Gly Ala Glu Gly Ala Gly Lys Gly Val Leu Phe Phe Arg Asn Arg Asn 
    450                 455                 460                 


Asn Val Gly Thr Pro Pro Ala Val Ile Lys Pro Val Ala 
465                 470                 475         


<210>  31
<211>  777
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(777)
<223>  synthetic delta-9 elongase (codon-optimized for Yarrowia 
       lipolytica)

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  WO 2007/061742
<311>  2006-11-16
<312>  2007-05-31
<313>  (1)..(777)

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  US-2007-0117190-A1
<311>  2006-11-16
<312>  2007-05-24
<313>  (1)..(777)

<400>  31
atg gag gtc gtg aac gaa atc gtc tcc att ggc cag gag gtt ctt ccc       48
Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro         
1               5                   10                  15              

aag gtc gac tat gct cag ctc tgg tct gat gcc tcg cac tgc gag gtg       96
Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val         
            20                  25                  30                  

ctg tac ctc tcc atc gcc ttc gtc atc ctg aag ttc acc ctt ggt cct      144
Leu Tyr Leu Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro         
        35                  40                  45                      

ctc gga ccc aag ggt cag tct cga atg aag ttt gtg ttc acc aac tac      192
Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr         
    50                  55                  60                          

aac ctg ctc atg tcc atc tac tcg ctg ggc tcc ttc ctc tct atg gcc      240
Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala         
65                  70                  75                  80          

tac gcc atg tac acc att ggt gtc atg tcc gac aac tgc gag aag gct      288
Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala         
                85                  90                  95              

ttc gac aac aat gtc ttc cga atc acc act cag ctg ttc tac ctc agc      336
Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser         
            100                 105                 110                 

aag ttc ctc gag tac att gac tcc ttc tat ctg ccc ctc atg ggc aag      384
Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys         
        115                 120                 125                     

cct ctg acc tgg ttg cag ttc ttt cac cat ctc gga gct cct atg gac      432
Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp         
    130                 135                 140                         

atg tgg ctg ttc tac aac tac cga aac gaa gcc gtt tgg atc ttt gtg      480
Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val         
145                 150                 155                 160         

ctg ctc aac ggc ttc att cac tgg atc atg tac ggc tac tat tgg acc      528
Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr         
                165                 170                 175             

cga ctg atc aag ctc aag ttc cct atg ccc aag tcc ctg att act tct      576
Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser         
            180                 185                 190                 

atg cag atc att cag ttc aac gtt ggc ttc tac atc gtc tgg aag tac      624
Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr         
        195                 200                 205                     

cgg aac att ccc tgc tac cga caa gat gga atg aga atg ttt ggc tgg      672
Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp         
    210                 215                 220                         

ttt ttc aac tac ttc tac gtt ggt act gtc ctg tgt ctg ttc ctc aac      720
Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn         
225                 230                 235                 240         

ttc tac gtg cag acc tac atc gtc cga aag cac aag gga gcc aaa aag      768
Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys         
                245                 250                 255             

att cag tga                                                          777
Ile Gln                                                                 
                                                                        


<210>  32
<211>  258
<212>  PRT
<213>  Euglena gracilis

<400>  32

Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro 
1               5                   10                  15      


Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val 
            20                  25                  30          


Leu Tyr Leu Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro 
        35                  40                  45              


Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr 
    50                  55                  60                  


Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala 
65                  70                  75                  80  


Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala 
                85                  90                  95      


Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser 
            100                 105                 110         


Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys 
        115                 120                 125             


Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp 
    130                 135                 140                 


Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val 
145                 150                 155                 160 


Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr 
                165                 170                 175     


Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 
        195                 200                 205             


Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp 
    210                 215                 220                 


Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys 
                245                 250                 255     


Ile Gln 
        


<210>  33
<211>  34
<212>  DNA
<213>  Escherichia coli

<400>  33
ataacttcgt ataatgtatg ctatacgaag ttat                                 34


<210>  34
<211>  828
<212>  DNA
<213>  Mortierella alpina


<220>
<221>  CDS
<222>  (1)..(828)
<223>  synthetic C16/18 elongase (codon-optimized for Yarrowia 
       lipolytica)

<300>
<302>  A MORTIERELLA ALPINA C16/18 FATTY ACID ELONGASE
<310>  US 2007-0087420-A1
<311>  2005-10-19
<312>  2007-04-19
<313>  (1)..(828)

<300>
<302>  A MORTIERELLA ALPINA C16/18 FATTY ACID ELONGASE
<310>  WO 2007/046817
<311>  2005-11-04
<312>  2007-04-26
<313>  (1)..(828)

<400>  34
atg gag tct gga ccc atg cct gct ggc att ccc ttc cct gag tac tat       48
Met Glu Ser Gly Pro Met Pro Ala Gly Ile Pro Phe Pro Glu Tyr Tyr         
1               5                   10                  15              

gac ttc ttt atg gac tgg aag act ccc ctg gcc atc gct gcc acc tac       96
Asp Phe Phe Met Asp Trp Lys Thr Pro Leu Ala Ile Ala Ala Thr Tyr         
            20                  25                  30                  

act gct gcc gtc ggt ctc ttc aac ccc aag gtt ggc aag gtc tcc cga      144
Thr Ala Ala Val Gly Leu Phe Asn Pro Lys Val Gly Lys Val Ser Arg         
        35                  40                  45                      

gtg gtt gcc aag tcg gct aac gca aag cct gcc gag cga acc cag tcc      192
Val Val Ala Lys Ser Ala Asn Ala Lys Pro Ala Glu Arg Thr Gln Ser         
    50                  55                  60                          

gga gct gcc atg act gcc ttc gtc ttt gtg cac aac ctc att ctg tgt      240
Gly Ala Ala Met Thr Ala Phe Val Phe Val His Asn Leu Ile Leu Cys         
65                  70                  75                  80          

gtc tac tct ggc atc acc ttc tac tac atg ttt cct gct atg gtc aag      288
Val Tyr Ser Gly Ile Thr Phe Tyr Tyr Met Phe Pro Ala Met Val Lys         
                85                  90                  95              

aac ttc cga acc cac aca ctg cac gaa gcc tac tgc gac acg gat cag      336
Asn Phe Arg Thr His Thr Leu His Glu Ala Tyr Cys Asp Thr Asp Gln         
            100                 105                 110                 

tcc ctc tgg aac aac gca ctt ggc tac tgg ggt tac ctc ttc tac ctg      384
Ser Leu Trp Asn Asn Ala Leu Gly Tyr Trp Gly Tyr Leu Phe Tyr Leu         
        115                 120                 125                     

tcc aag ttc tac gag gtc att gac acc atc atc atc atc ctg aag gga      432
Ser Lys Phe Tyr Glu Val Ile Asp Thr Ile Ile Ile Ile Leu Lys Gly         
    130                 135                 140                         

cga cgg tcc tcg ctg ctt cag acc tac cac cat gct gga gcc atg att      480
Arg Arg Ser Ser Leu Leu Gln Thr Tyr His His Ala Gly Ala Met Ile         
145                 150                 155                 160         

acc atg tgg tct ggc atc aac tac caa gcc act ccc att tgg atc ttt      528
Thr Met Trp Ser Gly Ile Asn Tyr Gln Ala Thr Pro Ile Trp Ile Phe         
                165                 170                 175             

gtg gtc ttc aac tcc ttc att cac acc atc atg tac tgt tac tat gcc      576
Val Val Phe Asn Ser Phe Ile His Thr Ile Met Tyr Cys Tyr Tyr Ala         
            180                 185                 190                 

ttc acc tct atc gga ttc cat cct cct ggc aaa aag tac ctg act tcg      624
Phe Thr Ser Ile Gly Phe His Pro Pro Gly Lys Lys Tyr Leu Thr Ser         
        195                 200                 205                     

atg cag att act cag ttt ctg gtc ggt atc acc att gcc gtg tcc tac      672
Met Gln Ile Thr Gln Phe Leu Val Gly Ile Thr Ile Ala Val Ser Tyr         
    210                 215                 220                         

ctc ttc gtt cct ggc tgc atc cga aca ccc ggt gct cag atg gct gtc      720
Leu Phe Val Pro Gly Cys Ile Arg Thr Pro Gly Ala Gln Met Ala Val         
225                 230                 235                 240         

tgg atc aac gtc ggc tac ctg ttt ccc ttg acc tat ctg ttc gtg gac      768
Trp Ile Asn Val Gly Tyr Leu Phe Pro Leu Thr Tyr Leu Phe Val Asp         
                245                 250                 255             

ttt gcc aag cga acc tac tcc aag cga tct gcc att gcc gct cag aaa      816
Phe Ala Lys Arg Thr Tyr Ser Lys Arg Ser Ala Ile Ala Ala Gln Lys         
            260                 265                 270                 

aag gct cag taa                                                      828
Lys Ala Gln                                                             
        275                                                             


<210>  35
<211>  275
<212>  PRT
<213>  Mortierella alpina

<400>  35

Met Glu Ser Gly Pro Met Pro Ala Gly Ile Pro Phe Pro Glu Tyr Tyr 
1               5                   10                  15      


Asp Phe Phe Met Asp Trp Lys Thr Pro Leu Ala Ile Ala Ala Thr Tyr 
            20                  25                  30          


Thr Ala Ala Val Gly Leu Phe Asn Pro Lys Val Gly Lys Val Ser Arg 
        35                  40                  45              


Val Val Ala Lys Ser Ala Asn Ala Lys Pro Ala Glu Arg Thr Gln Ser 
    50                  55                  60                  


Gly Ala Ala Met Thr Ala Phe Val Phe Val His Asn Leu Ile Leu Cys 
65                  70                  75                  80  


Val Tyr Ser Gly Ile Thr Phe Tyr Tyr Met Phe Pro Ala Met Val Lys 
                85                  90                  95      


Asn Phe Arg Thr His Thr Leu His Glu Ala Tyr Cys Asp Thr Asp Gln 
            100                 105                 110         


Ser Leu Trp Asn Asn Ala Leu Gly Tyr Trp Gly Tyr Leu Phe Tyr Leu 
        115                 120                 125             


Ser Lys Phe Tyr Glu Val Ile Asp Thr Ile Ile Ile Ile Leu Lys Gly 
    130                 135                 140                 


Arg Arg Ser Ser Leu Leu Gln Thr Tyr His His Ala Gly Ala Met Ile 
145                 150                 155                 160 


Thr Met Trp Ser Gly Ile Asn Tyr Gln Ala Thr Pro Ile Trp Ile Phe 
                165                 170                 175     


Val Val Phe Asn Ser Phe Ile His Thr Ile Met Tyr Cys Tyr Tyr Ala 
            180                 185                 190         


Phe Thr Ser Ile Gly Phe His Pro Pro Gly Lys Lys Tyr Leu Thr Ser 
        195                 200                 205             


Met Gln Ile Thr Gln Phe Leu Val Gly Ile Thr Ile Ala Val Ser Tyr 
    210                 215                 220                 


Leu Phe Val Pro Gly Cys Ile Arg Thr Pro Gly Ala Gln Met Ala Val 
225                 230                 235                 240 


Trp Ile Asn Val Gly Tyr Leu Phe Pro Leu Thr Tyr Leu Phe Val Asp 
                245                 250                 255     


Phe Ala Lys Arg Thr Tyr Ser Lys Arg Ser Ala Ile Ala Ala Gln Lys 
            260                 265                 270         


Lys Ala Gln 
        275 


<210>  36
<211>  8739
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pY116

<400>  36
ggccgccacc gcggcccgag attccggcct cttcggccgc caagcgaccc gggtggacgt     60

ctagaggtac ctagcaatta acagatagtt tgccggtgat aattctctta acctcccaca    120

ctcctttgac ataacgattt atgtaacgaa actgaaattt gaccagatat tgtgtccgcg    180

gtggagctcc agcttttgtt ccctttagtg agggtttaaa cgagcttggc gtaatcatgg    240

tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa cgtacgagcc    300

ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg    360

ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc    420

ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact    480

gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta    540

atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag    600

caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc    660

cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta    720

taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg    780

ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc    840

tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac    900

gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac    960

ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg   1020

aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga   1080

aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt   1140

agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag   1200

cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct   1260

gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg   1320

atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat   1380

gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc   1440

tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg   1500

gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct   1560

ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca   1620

actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg   1680

ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg   1740

tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc   1800

cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag   1860

ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg   1920

ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag   1980

tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat   2040

agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg   2100

atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca   2160

gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca   2220

aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat   2280

tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag   2340

aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc   2400

tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt   2460

gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc   2520

ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta   2580

cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc   2640

tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg   2700

ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt   2760

ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat   2820

tttaacaaaa tattaacgct tacaatttcc attcgccatt caggctgcgc aactgttggg   2880

aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg   2940

caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg   3000

ccagtgaatt gtaatacgac tcactatagg gcgaattggg taccgggccc cccctcgagg   3060

tcgatggtgt cgataagctt gatatcgaat tcatgtcaca caaaccgatc ttcgcctcaa   3120

ggaaacctaa ttctacatcc gagagactgc cgagatccag tctacactga ttaattttcg   3180

ggccaataat ttaaaaaaat cgtgttatat aatattatat gtattatata tatacatcat   3240

gatgatactg acagtcatgt cccattgcta aatagacaga ctccatctgc cgcctccaac   3300

tgatgttctc aatatttaag gggtcatctc gcattgttta ataataaaca gactccatct   3360

accgcctcca aatgatgttc tcaaaatata ttgtatgaac ttatttttat tacttagtat   3420

tattagacaa cttacttgct ttatgaaaaa cacttcctat ttaggaaaca atttataatg   3480

gcagttcgtt catttaacaa tttatgtaga ataaatgtta taaatgcgta tgggaaatct   3540

taaatatgga tagcataaat gatatctgca ttgcctaatt cgaaatcaac agcaacgaaa   3600

aaaatccctt gtacaacata aatagtcatc gagaaatatc aactatcaaa gaacagctat   3660

tcacacgtta ctattgagat tattattgga cgagaatcac acactcaact gtctttctct   3720

cttctagaaa tacaggtaca agtatgtact attctcattg ttcatacttc tagtcatttc   3780

atcccacata ttccttggat ttctctccaa tgaatgacat tctatcttgc aaattcaaca   3840

attataataa gatataccaa agtagcggta tagtggcaat caaaaagctt ctctggtgtg   3900

cttctcgtat ttatttttat tctaatgatc cattaaaggt atatatttat ttcttgttat   3960

ataatccttt tgtttattac atgggctgga tacataaagg tattttgatt taattttttg   4020

cttaaattca atcccccctc gttcagtgtc aactgtaatg gtaggaaatt accatacttt   4080

tgaagaagca aaaaaaatga aagaaaaaaa aaatcgtatt tccaggttag acgttccgca   4140

gaatctagaa tgcggtatgc ggtacattgt tcttcgaacg taaaagttgc gctccctgag   4200

atattgtaca tttttgcttt tacaagtaca agtacatcgt acaactatgt actactgttg   4260

atgcatccac aacagtttgt tttgtttttt tttgtttttt ttttttctaa tgattcatta   4320

ccgctatgta tacctacttg tacttgtagt aagccgggtt attggcgttc aattaatcat   4380

agacttatga atctgcacgg tgtgcgctgc gagttacttt tagcttatgc atgctacttg   4440

ggtgtaatat tgggatctgt tcggaaatca acggatgctc aaccgatttc gacagtaatt   4500

aattaatttg aatcgaatcg gagcctaaaa tgaacccgag tatatctcat aaaattctcg   4560

gtgagaggtc tgtgactgtc agtacaaggt gccttcatta tgccctcaac cttaccatac   4620

ctcactgaat gtagtgtacc tctaaaaatg aaatacagtg ccaaaagcca aggcactgag   4680

ctcgtctaac ggacttgata tacaaccaat taaaacaaat gaaaagaaat acagttcttt   4740

gtatcatttg taacaattac cctgtacaaa ctaaggtatt gaaatcccac aatattccca   4800

aagtccaccc ctttccaaat tgtcatgcct acaactcata taccaagcac taacctacca   4860

aacaccacta aaaccccaca aaatatatct taccgaatat acagtaacaa gctaccacca   4920

cactcgttgg gtgcagtcgc cagcttaaag atatctatcc acatcagcca caactccctt   4980

cctttaataa accgactaca cccttggcta ttgaggttat gagtgaatat actgtagaca   5040

agacactttc aagaagactg tttccaaaac gtaccactgt cctccactac aaacacaccc   5100

aatctgcttc ttctagtcaa ggttgctaca ccggtaaatt ataaatcatc atttcattag   5160

cagggcaggg ccctttttat agagtcttat acactagcgg accctgccgg tagaccaacc   5220

cgcaggcgcg tcagtttgct ccttccatca atgcgtcgta gaaacgactt actccttctt   5280

gagcagctcc ttgaccttgt tggcaacaag tctccgacct cggaggtgga ggaagagcct   5340

ccgatatcgg cggtagtgat accagcctcg acggactcct tgacggcagc ctcaacagcg   5400

tcaccggcgg gcttcatgtt aagagagaac ttgagcatca tggcggcaga cagaatggtg   5460

gcaatggggt tgaccttctg cttgccgaga tcgggggcag atccgtgaca gggctcgtac   5520

agaccgaacg cctcgttggt gtcgggcaga gaagccagag aggcggaggg cagcagaccc   5580

agagaaccgg ggatgacgga ggcctcgtcg gagatgatat cgccaaacat gttggtggtg   5640

atgatgatac cattcatctt ggagggctgc ttgatgagga tcatggcggc cgagtcgatc   5700

agctggtggt tgagctcgag ctgggggaat tcgtccttga ggactcgagt gacagtcttt   5760

cgccaaagtc gagaggaggc cagcacgttg gccttgtcaa gagaccacac gggaagaggg   5820

gggttgtgct gaagggccag gaaggcggcc attcgggcaa ttcgctcaac ctcaggaacg   5880

gagtaggtct cggtgtcgga agcgacgcca gatccgtcat cctcctttcg ctctccaaag   5940

tagatacctc cgacgagctc tcggacaatg atgaagtcgg tgccctcaac gtttcggatg   6000

ggggagagat cggcgagctt gggcgacagc agctggcagg gtcgcaggtt ggcgtacagg   6060

ttcaggtcct ttcgcagctt gaggagaccc tgctcgggtc gcacgtcggt tcgtccgtcg   6120

ggagtggtcc atacggtgtt ggcagcgcct ccgacagcac cgagcataat agagtcagcc   6180

tttcggcaga tgtcgagagt agcgtcggtg atgggctcgc cctccttctc aatggcagct   6240

cctccaatga gtcggtcctc aaacacaaac tcggtgccgg aggcctcagc aacagacttg   6300

agcaccttga cggcctcggc aatcacctcg gggccacaga agtcgccgcc gagaagaaca   6360

atcttcttgg agtcagtctt ggtcttctta gtttcgggtt ccattgtgga tgtgtgtggt   6420

tgtatgtgtg atgtggtgtg tggagtgaaa atctgtggct ggcaaacgct cttgtatata   6480

tacgcacttt tgcccgtgct atgtggaaga ctaaacctcc gaagattgtg actcaggtag   6540

tgcggtatcg gctagggacc caaaccttgt cgatgccgat agcgctatcg aacgtacccc   6600

agccggccgg gagtatgtcg gaggggacat acgagatcgt caagggtttg tggccaactg   6660

gtatttaaat gtagctaacg gtagcaggcg aactactggt acatacctcc cccggaatat   6720

gtacaggcat aatgcgtatc tgtgggacat gtggtcgttg cgccattatg taagcagcgt   6780

gtactcctct gactgtccat atggtttgct ccatctcacc ctcatcgttt tcattgttca   6840

caggcggcca caaaaaaact gtcttctctc cttctctctt cgccttagtc tactcggacc   6900

agttttagtt tagcttggcg ccactggata aatgagacct caggccttgt gatgaggagg   6960

tcacttatga agcatgttag gaggtgcttg tatggataga gaagcaccca aaataataag   7020

aataataata aaacaggggg cgttgtcatt tcatatcgtg ttttcaccat caatacacct   7080

ccaaacaatg cccttcatgt ggccagcccc aatattgtcc tgtagttcaa ctctatgcag   7140

ctcgtatctt attgagcaag taaaactctg tcagccgata ttgcccgacc cgcgacaagg   7200

gtcaacaagg tggtgtaagg ccttcgcaga agtcaaaact gtgccaaaca aacatctaga   7260

gtctctttgg tgtttctcgc atatatttwa tcggctgtct tacgtatttg cgcctcggta   7320

ccggactaat ttcggatcat ccccaatacg ctttttcttc gcagctgtca acagtgtcca   7380

tgatctatcc acctaaatgg gtcatatgag gcgtataatt tcgtggtgct gataataatt   7440

cccatatatt tgacacaaaa cttccccccc tagacataca tctcacaatc tcacttcttg   7500

tgcttctgtc acacatctcc tccagctgac ttcaactcac acctctgccc cagttggtct   7560

acagcggtat aaggtttctc cgcatagagg tgcaccactc ctcccgatac ttgtttgtgt   7620

gacttgtggg tcacgacata tatatctaca cacattgcgc caccctttgg ttcttccagc   7680

acaacaaaaa cacgacacgc taaccatggc caatttactg accgtacacc aaaatttgcc   7740

tgcattaccg gtcgatgcaa cgagtgatga ggttcgcaag aacctgatgg acatgttcag   7800

ggatcgccag gcgttttctg agcatacctg gaaaatgctt ctgtccgttt gccggtcgtg   7860

ggcggcatgg tgcaagttga ataaccggaa atggtttccc gcagaacctg aagatgttcg   7920

cgattatctt ctatatcttc aggcgcgcgg tctggcagta aaaactatcc agcaacattt   7980

gggccagcta aacatgcttc atcgtcggtc cgggctgcca cgaccaagtg acagcaatgc   8040

tgtttcactg gttatgcggc ggatccgaaa agaaaacgtt gatgccggtg aacgtgcaaa   8100

acaggctcta gcgttcgaac gcactgattt cgaccaggtt cgttcactca tggaaaatag   8160

cgatcgctgc caggatatac gtaatctggc atttctgggg attgcttata acaccctgtt   8220

acgtatagcc gaaattgcca ggatcagggt taaagatatc tcacgtactg acggtgggag   8280

aatgttaatc catattggca gaacgaaaac gctggttagc accgcaggtg tagagaaggc   8340

acttagcctg ggggtaacta aactggtcga gcgatggatt tccgtctctg gtgtagctga   8400

tgatccgaat aactacctgt tttgccgggt cagaaaaaat ggtgttgccg cgccatctgc   8460

caccagccag ctatcaactc gcgccctgga agggattttt gaagcaactc atcgattgat   8520

ttacggcgct aaggatgact ctggtcagag atacctggcc tggtctggac acagtgcccg   8580

tgtcggagcc gcgcgagata tggcccgcgc tggagtttca ataccggaga tcatgcaagc   8640

tggtggctgg accaatgtaa atattgtcat gaactatatc cgtaacctgg atagtgaaac   8700

aggggcaatg gtgcgcctgc tggaagatgg cgattaagc                          8739


<210>  37
<211>  15337
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pKO2UF8289

<400>  37
cgatcgagga agaggacaag cggctgcttc ttaagtttgt gacatcagta tccaaggcac     60

cattgcaagg attcaaggct ttgaacccgt catttgccat tcgtaacgct ggtagacagg    120

ttgatcggtt ccctacggcc tccacctgtg tcaatcttct caagctgcct gactatcagg    180

acattgatca acttcggaag aaacttttgt atgccattcg atcacatgct ggtttcgatt    240

tgtcttagag gaacgcatat acagtaatca tagagaataa acgatattca tttattaaag    300

tagatagttg aggtagaagt tgtaaagagt gataaatagc ggccgctcac tgaatctttt    360

tggctccctt gtgcttcctg acgatatacg tttgcacata gaaattcaag aacaaacaca    420

agactgtgcc aacataaaag taattgaaga accagccaaa catcctcatc ccatcttggc    480

gataacaggg aatgttcctg tacttccaga caatgtagaa accaacattg aattgaatga    540

tctgcattga tgtaatcagg gattttggca tggggaactt cagcttgatc aatctggtcc    600

aataataacc gtacatgatc cagtggatga aaccattcaa cagcacaaaa atccaaacag    660

cttcatttcg gtaattatag aacagccaca tatccatcgg tgcccccaaa tgatggaaga    720

attgcaacca ggtcagaggc ttgcccatca gtggcaaata gaaggagtca atatactcca    780

ggaacttgct caaatagaac aactgcgtgg tgatcctgaa gacgttgttg tcaaaagcct    840

tctcgcagtt gtcagacata acaccgatgg tgtacatggc atatgccatt gagaggaatg    900

atcccaacga ataaatggac atgagaaggt tgtaattggt gaaaacaaac ttcatacgag    960

actgaccttt tggaccaagg gggccaagag tgaacttcaa gatgacaaat gcgatggaca   1020

agtaaagcac ctcacagtga ctggcatcac tccagagttg ggcataatca actggttggg   1080

taaaacttcc tgcccaattg agactatttc attcaccacc tccatggtta gcgtgtcgtg   1140

tttttgttgt gctggaagaa ccaaagggtg gcgcaatgtg tgtagatata tatgtcgtga   1200

cccacaagtc acacaaacaa gtatcgggag gagtggtgca cctctatgcg gagaaacctt   1260

ataccgctgt agaccaactg gggcagaggt gtgagttgaa gtcagctgga ggagatgtgt   1320

gacagaagca caagaagtga gattgtgaga tgtatgtcta gggggggaag ttttgtgtca   1380

aatatatggg aattattatc agcaccacga aattatacgc ctcatatgac ccatttaggt   1440

ggatagatca tggacactgt tgacagctgc gaagaaaaag cgtattgggg atgatccgaa   1500

attagtccgg taccgaggcg caaatacgta agacagccga twaaatatat gcgagaaaca   1560

ccaaagagac tctagatgtt tgtttggcac agttttgact tctgcgaagg ccttacacca   1620

ccttgttgac ccttgtcgcg ggtcgggcaa tatcggctga cagagtttta cttgctcaat   1680

aagatacgag ctgcatagag ttgaactaca ggacaatatt ggggctggcc acatgaaggg   1740

cattgtttgg aggtgtattg atggtgaaaa cacgatatga aatgacaacg ccccctgttt   1800

tattattatt cttattattt tgggtgcttc tctatccata caagcacctc ctaacatgct   1860

tcataagtga cctcctcatc acaaggcctg aggtctcatt tatccagtgg cgccaagcta   1920

aactaaaact ggtccgagta gactaaggcg aagagagaag gagagaagac agtttttttg   1980

tggccgcctg tgaacaatga aaacgatgag ggtgagatgg agcaaaccat atggtttaaa   2040

cagtcagagg agtacacgct gcttacataa tggcgcaacg accacatgtc ccacagatac   2100

gcatcgattc gattcaaatt aattaaaagg cgttgaaaca gaatgagcca gacagcaagg   2160

acaaggtggc caacagcaag gagtccaaaa agccctctat tgacgagatc cacgatgtta   2220

ttgctcatga ggtttccgag ctcgatgctg ggaagaagaa gtgatttgta tataagaaat   2280

aaatgagata tagtaaagga gtgcaagaga atggcaaggt ggtcaaattc tatattactt   2340

gcagtcactg gttcctcgtt gacatgaatg aagttaccgt tggcatagct gatttaatat   2400

ataactgtcc aactaactct cacctagata taacccatgt gtgtgtttcc aatcatcaat   2460

gcggccgctt actgagcctt ggcaccgggc tgcttctcgg ccattcgagc gaactgggac   2520

aggtatcgga gcaggatgac gagaccttca tggggcagag ggtttcggta ggggaggttg   2580

tgcttctggc acagctgttc cacctggtag gaaacggcag tgaggttgtg tcgaggcagg   2640

gtgggccaga gatggtgctc gatctggtag ttcaggcctc caaagaacca gtcagtaatg   2700

atgcctcgtc gaatgttcat ggtctcatgg atctgaccca cagagaagcc atgtccgtcc   2760

cagacggaat caccgatctt ctccagaggg tagtggttca tgaagaccac gatggcaatt   2820

ccgaagccac cgacgagctc ggaaacaaag aacaccagca tcgaggtcag gatggagggc   2880

ataaagaaga ggtggaacag ggtcttgaga gtccagtgca gagcgagtcc aatggcctct   2940

ttcttgtact gagatcggta gaactggttg tctcggtcct tgagggatcg aacggtcagc   3000

acagactgga aacaccagat gaatcgcagg agaatacaga tgaccaggaa atagtactgt   3060

tggaactgaa tgagctttcg ggagatggga gaagctcgag tgacatcgtc ctcggaccag   3120

gcgagcagag gcaggttatc aatgtcggga tcgtgaccct gaacgttggt agcagaatga   3180

tgggcgttgt gtctgtcctt ccaccaggtc acggagaagc cctggagtcc gttgccaaag   3240

accagaccca ggacgttatt ccagtttcgg ttcttgaagg tctggtggtg gcagatgtca   3300

tgagacagcc atcccatttg ctggtagtgc ataccgagca cgagagcacc aatgaagtac   3360

aggtggtact ggaccagcat gaagaaggca agcacgccaa gacccagggt ggtcaagatc   3420

ttgtacgagt accagagggg agaggcgtca aacatgccag tggcgatcag ctcttctcgg   3480

agctttcgga aatcctcctg agcttcgttg acggcagcct ggggaggcag ctcggaagcc   3540

tggttgatct tgggcattcg cttgagcttg tcgaaggctt cctgagagtg cataaccatg   3600

aaggcgtcag tagcatctcg tccctggtag ttctcaatga tttcagctcc accagggtgg   3660

aagttcaccc aagcggagac gtcgtacacc tttccgtcga tgacgagggg cagagcctgt   3720

cgagaagcct tcaccatggc cattgctgta gatatgtctt gtgtgtaagg gggttggggt   3780

ggttgtttgt gttcttgact tttgtgttag caagggaaga cgggcaaaaa agtgagtgtg   3840

gttgggaggg agagacgagc cttatatata atgcttgttt gtgtttgtgc aagtggacgc   3900

cgaaacgggc aggagccaaa ctaaacaagg cagacaatgc gagcttaatt ggattgcctg   3960

atgggcaggg gttagggctc gatcaatggg ggtgcgaagt gacaaaattg ggaattaggt   4020

tcgcaagcaa ggctgacaag actttggccc aaacatttgt acgcggtgga caacaggagc   4080

cacccatcgt ctgtcacggg ctagccggtc gtgcgtcctg tcaggctcca cctaggctcc   4140

atgccactcc atacaatccc actagtgtac cgctaggccg cttttagctc ccatctaaga   4200

cccccccaaa acctccactg tacagtgcac tgtactgtgt ggcgatcaag ggcaagggaa   4260

aaaaggcgca aacatgcacg catggaatga cgtaggtaag gcgttactag actgaaaagt   4320

ggcacatttc ggcgtgccaa agggtcctag gtgcgtttcg cgagctgggc gccaggccaa   4380

gccgctccaa aacgcctctc cgactccctc cagcggcctc catatcccca tccctctcca   4440

cagcaatgtt gttaagcctt gcaaacgaaa aaatagaaag gctaataagc ttccaatatt   4500

gtggtgtacg ctgcataacg caacaatgag cgccaaacaa cacacacaca cagcacacag   4560

cagcattaac cacgatgttt aaacagtgta cgcagatccc gtcaacagtt ttatatatcg   4620

tagttacaac catcaacact ttttggtaag tgtaccattc tatactccaa ctggtctgca   4680

actgtacaag tagacatgtt aatggtagtt aataacatct acagcagaac ctatggtaaa   4740

gacattgcat ttttacagga agtatcgtcc tacacgttga taaatccaaa gatgcggaac   4800

ttcttccact tttatcatca tcccctactc gtacactcgt actctttgtt cgatcgcgat   4860

tcatttctat aaataatctt gtatgtacat gcggccgctt aagcaacggg cttgataaca   4920

gcgggggggg tgcccacgtt gttgcggttg cggaagaaca gaacaccctt accagcaccc   4980

tcggcaccag cgctgggctc aacccactgg cacatacgcg cactgcggta catggcgcgg   5040

atgaagccac gaggaccatc ctggacatca gcccggtagt gcttgcccat gatgggctta   5100

atggcctcgg tggcctcgtc cgcgttgtag aaggggatgc tgctgacgta gtggtggagg   5160

acatgagtct cgatgatgcc gtggagaagg tggcggccga tgaagcccat ctcacggtca   5220

atggtagcag cggcaccacg gacgaagttc cactcgtcgt tggtgtagtg gggaagggta   5280

gggtcggtgt gctggaggaa ggtgatggca acgagccagt ggttaaccca gaggtaggga   5340

acaaagtacc agatggccat gttgtagaaa ccgaacttct gaacgaggaa gtacagagca   5400

gtggccatca gaccgatacc aatatcgctg aggacgatga gcttagcgtc actgttctcg   5460

tacagagggc tgcggggatc gaagtggtta acaccaccgc cgaggccgtt atgcttgccc   5520

ttgccgcgac cctcacgctg gcgctcgtgg tagttgtggc cggtaacatt ggtgatgagg   5580

tagttgggcc agccaacgag ctgctgaagg acgagcatga gaagagtgaa agcgggggtc   5640

tcctcagtaa gatgagcgag ctcgtgggtc atctttccga gacgagtagc ctgctgctcg   5700

cgggttcggg gaacgaagac catgtcacgc tccatgttgc cagtggcctt gtggtgcttt   5760

cggtgggaga tttgccagct gaagtagggg acaaggaggg aagagtgaag aacccagcca   5820

gtaatgtcgt tgatgatgcg agaatcggag aaagcaccgt gaccgcactc atgggcaata   5880

acccagagac cagtaccgaa aagaccctga agaacggtgt acacggccca cagaccagcg   5940

cgggcggggg tggaggggat atattcgggg gtcacaaagt tgtaccagat gctgaaagtg   6000

gtagtcagga ggacaatgtc gcggaggata taaccgtatc ccttgagagc ggagcgcttg   6060

aagcagtgct tagggatggc attgtagatg tccttgatgg taaagtcggg aacctcgaac   6120

tggttgccgt aggtgtcgag catgacacca tactcggact tgggcttggc gatatcaacc   6180

tcggacatgg acgagagcga tgtggaagag gccgagtggc ggggagagtc tgaaggagag   6240

acggcggcag actcagaatc cgtcacagta gttgaggtga cggtgcgtct aagcgcaggg   6300

ttctgcttgg gcagagccga agtggacgcc atggttgtga attagggtgg tgagaatggt   6360

tggttgtagg gaagaatcaa aggccggtct cgggatccgt gggtatatat atatatatat   6420

atatatacga tccttcgtta cctccctgtt ctcaaaactg tggtttttcg tttttcgttt   6480

tttgcttttt ttgatttttt tagggccaac taagcttcca gatttcgcta atcacctttg   6540

tactaattac aagaaaggaa gaagctgatt agagttgggc tttttatgca actgtgctac   6600

tccttatctc tgatatgaaa gtgtagaccc aatcacatca tgtcatttag agttggtaat   6660

actgggagga tagataaggc acgaaaacga gccatagcag acatgctggg tgtagccaag   6720

cagaagaaag tagatgggag ccaattgacg agcgagggag ctacgccaat ccgacatacg   6780

acacgctgag atcgtcttgg ccggggggta cctacagatg tccaagggta agtgcttgac   6840

tgtaattgta tgtctgagga caaatatgta gtcagccgta taaagtcata ccaggcacca   6900

gtgccatcat cgaaccacta actctctatg atacatgcct ccggtattat tgtaccatgc   6960

gtcgctttgt tacatacgta tcttgccttt ttctctcaga aactccagac tttggctatt   7020

ggtcgagata agcccggacc atagtgagtc tttcacactc tacatttctc ccttgctcca   7080

actatttaaa ttgccccgga gaagacggcc aggccgccta gatgacaaat tcaacaactc   7140

acagctgact ttctgccatt gccactaggg gggggccttt ttatatggcc aagccaagct   7200

ctccacgtcg gttgggctgc acccaacaat aaatgggtag ggttgcacca acaaagggat   7260

gggatggggg gtagaagata cgaggataac ggggctcaat ggcacaaata agaacgaata   7320

ctgccattaa gactcgtgat ccagcgactg acaccattgc atcatctaag ggcctcaaaa   7380

ctacctcgga actgctgcgc tgatctggac accacagagg ttccgagcac tttaggttgc   7440

accaaatgtc ccaccaggtg caggcagaaa acgctggaac agcgtgtaca gtttgtctta   7500

acaaaaagtg agggcgctga ggtcgagcag ggtggtgtga cttgttatag cctttagagc   7560

tgcgaaagcg cgtatggatt tggctcatca ggccagattg agggtctgtg gacacatgtc   7620

atgttagtgt acttcaatcg ccccctggat atagccccga caataggccg tggcctcatt   7680

tttttgcctt ccgcacattt ccattgctcg gtacccacac cttgcttctc ctgcacttgc   7740

caaccttaat actggtttac attgaccaac atcttacaag cggggggctt gtctagggta   7800

tatataaaca gtggctctcc caatcggttg ccagtctctt ttttcctttc tttccccaca   7860

gattcgaaat ctaaactaca catcacagaa ttccgagccg tgagtatcca cgacaagatc   7920

agtgtcgaga cgacgcgttt tgtgtaatga cacaatccga aagtcgctag caacacacac   7980

tctctacaca aactaaccca gctctggtac catggtgaag gcttctcgac aggctctgcc   8040

cctcgtcatc gacggaaagg tgtacgacgt ctccgcttgg gtgaacttcc accctggtgg   8100

agctgaaatc attgagaact accagggacg agatgctact gacgccttca tggttatgca   8160

ctctcaggaa gccttcgaca agctcaagcg aatgcccaag atcaaccagg cttccgagct   8220

gcctccccag gctgccgtca acgaagctca ggaggatttc cgaaagctcc gagaagagct   8280

gatcgccact ggcatgtttg acgcctctcc cctctggtac tcgtacaaga tcttgaccac   8340

cctgggtctt ggcgtgcttg ccttcttcat gctggtccag taccacctgt acttcattgg   8400

tgctctcgtg ctcggtatgc actaccagca aatgggatgg ctgtctcatg acatctgcca   8460

ccaccagacc ttcaagaacc gaaactggaa taacgtcctg ggtctggtct ttggcaacgg   8520

actccagggc ttctccgtga cctggtggaa ggacagacac aacgcccatc attctgctac   8580

caacgttcag ggtcacgatc ccgacattga taacctgcct ctgctcgcct ggtccgagga   8640

cgatgtcact cgagcttctc ccatctcccg aaagctcatt cagttccaac agtactattt   8700

cctggtcatc tgtattctcc tgcgattcat ctggtgtttc cagtctgtgc tgaccgttcg   8760

atccctcaag gaccgagaca accagttcta ccgatctcag tacaagaaag aggccattgg   8820

actcgctctg cactggactc tcaagaccct gttccacctc ttctttatgc cctccatcct   8880

gacctcgatg ctggtgttct ttgtttccga gctcgtcggt ggcttcggaa ttgccatcgt   8940

ggtcttcatg aaccactacc ctctggagaa gatcggtgat tccgtctggg acggacatgg   9000

cttctctgtg ggtcagatcc atgagaccat gaacattcga cgaggcatca ttactgactg   9060

gttctttgga ggcctgaact accagatcga gcaccatctc tggcccaccc tgcctcgaca   9120

caacctcact gccgtttcct accaggtgga acagctgtgc cagaagcaca acctccccta   9180

ccgaaaccct ctgccccatg aaggtctcgt catcctgctc cgatacctgt cccagttcgc   9240

tcgaatggcc gagaagcagc ccggtgccaa ggctcagtaa gcggccgcaa gtgtggatgg   9300

ggaagtgagt gcccggttct gtgtgcacaa ttggcaatcc aagatggatg gattcaacac   9360

agggatatag cgagctacgt ggtggtgcga ggatatagca acggatattt atgtttgaca   9420

cttgagaatg tacgatacaa gcactgtcca agtacaatac taaacatact gtacatactc   9480

atactcgtac ccgggcaacg gtttcacttg agtgcagtgg ctagtgctct tactcgtaca   9540

gtgtgcaata ctgcgtatca tagtctttga tgtatatcgt attcattcat gttagttgcg   9600

tacgggtgaa gcttccactg gtcggcgtgg tagtggggca gagtggggtc ggtgtgctgc   9660

aggtaggtga tggccacgag ccagtggttg acccacaggt aggggatcag gtagtagagg   9720

gtgacggaag ccaggcccca tcggttgatg gagtatgcga tgacggacat ggtgatacca   9780

ataccgacgt tagagatcca gatgttgaac cagtccttct tctcaaacag cggggcgttg   9840

gggttgaagt ggttgacagc ccatttgttg agcttggggt acttctgtcc ggtaacgtaa   9900

gacagcagat acagaggcca tccaaacacc tgctgggtga tgaggccgta gagggtcatg   9960

aggggagcgt cctcagcaag ctcagaccag tcatgggcgc ctcggttctc cataaactcc  10020

tttcggtcct tgggcacaaa caccatatca cgggtgaggt gaccagtgga cttgtggtgc  10080

atggagtggg tcagcttcca ggcgtagtaa gggaccagca tggaggagtg cagaacccat  10140

ccggtgacgt tgttgacggt gttagagtcg gagaaagcag agtggccaca ctcgtgggca  10200

agaacccaca gaccggtgcc aaacagaccc tggacaatgg agtacatggc ccaggccaca  10260

gctcggccgg aagccgaggg aataagaggc aggtacgcgt aggccatgta ggcaaaaacg  10320

gcgataaaga agcaggcgcg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc  10380

ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt  10440

cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca  10500

ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa  10560

aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat  10620

cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc  10680

cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc  10740

gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt  10800

tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac  10860

cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg  10920

ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca  10980

gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc  11040

gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa  11100

accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa  11160

ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac  11220

tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta  11280

aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt  11340

taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata  11400

gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc  11460

agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac  11520

cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag  11580

tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac  11640

gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc  11700

agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg  11760

gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc  11820

atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct  11880

gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc  11940

tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc  12000

atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc  12060

agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc  12120

gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca  12180

cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt  12240

tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt  12300

ccgcgcacat ttccccgaaa agtgccacct gatgcggtgt gaaataccgc acagatgcgt  12360

aaggagaaaa taccgcatca ggaaattgta agcgttaata ttttgttaaa attcgcgtta  12420

aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat  12480

aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca  12540

ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc  12600

ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta  12660

aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg  12720

gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg  12780

gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc  12840

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat  12900

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt  12960

tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac tcactatagg  13020

gcgaattggg cccgacgtcg catgcttgaa tctacaagta ggagggttgg agtgattaag  13080

tgaaacttct ttaacggctc tatgccagtt ctattgatat ccgaaacatc agtatgaagg  13140

tctgataagg gtgacttctt cccacagatt cgtatcagta cgagtacgag accggtactt  13200

gtaacagtat tgatactaaa gggaaactac aacggttgtc agcgtaatgt gacttcgccc  13260

atgaacgcag acacgcagtg ccgagtgcgg tgatatcgcc tactcgttac gtccatggac  13320

tacacaaccc ctcggcttcg cttggcttag cctcgggctc ggtgctgttc agttaaaaca  13380

caatcaaata acatttctac tttttagaag gcaggccgtc aggagcaact ccgactccat  13440

tgacgtttct aaacatctga atgccttcct taccttcaac aaactggcag gttcgggcga  13500

cagtgtaaag agacttgatg aagttggtgt cgtcgtgtcg gtagtgcttg cccatgacct  13560

tcttgatctt ctcagtggcg attcgggcgt tgtagaaggg aattccttta cctgcaggat  13620

aacttcgtat aatgtatgct atacgaagtt atgatctctc tcttgagctt ttccataaca  13680

agttcttctg cctccaggaa gtccatgggt ggtttgatca tggttttggt gtagtggtag  13740

tgcagtggtg gtattgtgac tggggatgta gttgagaata agtcatacac aagtcagctt  13800

tcttcgagcc tcatataagt ataagtagtt caacgtatta gcactgtacc cagcatctcc  13860

gtatcgagaa acacaacaac atgccccatt ggacagatca tgcggataca caggttgtgc  13920

agtatcatac atactcgatc agacaggtcg tctgaccatc atacaagctg aacaagcgct  13980

ccatacttgc acgctctcta tatacacagt taaattacat atccatagtc taacctctaa  14040

cagttaatct tctggtaagc ctcccagcca gccttctggt atcgcttggc ctcctcaata  14100

ggatctcggt tctggccgta cagacctcgg ccgacaatta tgatatccgt tccggtagac  14160

atgacatcct caacagttcg gtactgctgt ccgagagcgt ctcccttgtc gtcaagaccc  14220

accccggggg tcagaataag ccagtcctca gagtcgccct taggtcggtt ctgggcaatg  14280

aagccaacca caaactcggg gtcggatcgg gcaagctcaa tggtctgctt ggagtactcg  14340

ccagtggcca gagagccctt gcaagacagc tcggccagca tgagcagacc tctggccagc  14400

ttctcgttgg gagaggggac taggaactcc ttgtactggg agttctcgta gtcagagacg  14460

tcctccttct tctgttcaga gacagtttcc tcggcaccag ctcgcaggcc agcaatgatt  14520

ccggttccgg gtacaccgtg ggcgttggtg atatcggacc actcggcgat tcggtgacac  14580

cggtactggt gcttgacagt gttgccaata tctgcgaact ttctgtcctc gaacaggaag  14640

aaaccgtgct taagagcaag ttccttgagg gggagcacag tgccggcgta ggtgaagtcg  14700

tcaatgatgt cgatatgggt tttgatcatg cacacataag gtccgacctt atcggcaagc  14760

tcaatgagct ccttggtggt ggtaacatcc agagaagcac acaggttggt tttcttggct  14820

gccacgagct tgagcactcg agcggcaaag gcggacttgt ggacgttagc tcgagcttcg  14880

taggagggca ttttggtggt gaagaggaga ctgaaataaa tttagtctgc agaacttttt  14940

atcggaacct tatctggggc agtgaagtat atgttatggt aatagttacg agttagttga  15000

acttatagat agactggact atacggctat cggtccaaat tagaaagaac gtcaatggct  15060

ctctgggcgt cgcctttgcc gacaaaaatg tgatcatgat gaaagccagc aatgacgttg  15120

cagctgatat tgttgtcggc caaccgcgcc gaaaacgcag ctgtcagacc cacagcctcc  15180

aacgaagaat gtatcgtcaa agtgatccaa gcacactcat agttggagtc gtactccaaa  15240

ggcggcaatg acgagtcaga cagatactcg tcgacgcgat aacttcgtat aatgtatgct  15300

atacgaagtt atcgtacgat agttagtaga caacaat                           15337


<210>  38
<211>  1936
<212>  DNA
<213>  Yarrowia lipolytica


<220>
<221>  CDS
<222>  (283)..(1539)

<300>
<302>  A  DELTA-12 DESATURASE GENE SUITABLE FOR ALTERING LEVELS OF 
       POLYUNSATURATED FATTY ACIDS IN OLEAGINOUS YEASTS
<310>  U.S. 7,214,491
<311>  2004-05-06
<312>  2007-05-08
<313>  (1)..(1936)

<300>
<302>  A  DELTA-12 DESATURASE GENE SUITABLE FOR ALTERING LEVELS OF 
       POLYUNSATURATED FATTY ACIDS IN OLEAGINOUS YEASTS
<310>  WO 2004/104167
<311>  2004-05-07
<312>  2004-12-02
<313>  (1)..(1936)

<400>  38
cgtagttata tacaagaggt agatgcgtgc tggtgttaga ggggctctca ggattaggag     60

gaaaatttga cattggccct caacatataa cctcgggtgt gcctctgttt accctcagct    120

tttgcttgtc cccaagtcag tcacgccagg ccaaaaaggt tggtggattg acagggagaa    180

aaaaaaaagc ctagtgggtt taaactcgag gtaagacatt gaaatatata ccggtcggca    240

tcctgagtcc ctttctcgta ttccaacaga ccgaccatag aa atg gat tcg acc       294
                                               Met Asp Ser Thr          
                                               1                        

acg cag acc aac acc ggc acc ggc aag gtg gcc gtg cag ccc ccc acg      342
Thr Gln Thr Asn Thr Gly Thr Gly Lys Val Ala Val Gln Pro Pro Thr         
5                   10                  15                  20          

gcc ttc att aag ccc att gag aag gtg tcc gag ccc gtc tac gac acc      390
Ala Phe Ile Lys Pro Ile Glu Lys Val Ser Glu Pro Val Tyr Asp Thr         
                25                  30                  35              

ttt ggc aac gag ttc act cct cca gac tac tct atc aag gat att ctg      438
Phe Gly Asn Glu Phe Thr Pro Pro Asp Tyr Ser Ile Lys Asp Ile Leu         
            40                  45                  50                  

gat gcc att ccc cag gag tgc tac aag cgg tcc tac gtt aag tcc tac      486
Asp Ala Ile Pro Gln Glu Cys Tyr Lys Arg Ser Tyr Val Lys Ser Tyr         
        55                  60                  65                      

tcg tac gtg gcc cga gac tgc ttc ttt atc gcc gtt ttt gcc tac atg      534
Ser Tyr Val Ala Arg Asp Cys Phe Phe Ile Ala Val Phe Ala Tyr Met         
    70                  75                  80                          

gcc tac gcg tac ctg cct ctt att ccc tcg gct tcc ggc cga gct gtg      582
Ala Tyr Ala Tyr Leu Pro Leu Ile Pro Ser Ala Ser Gly Arg Ala Val         
85                  90                  95                  100         

gcc tgg gcc atg tac tcc att gtc cag ggt ctg ttt ggc acc ggt ctg      630
Ala Trp Ala Met Tyr Ser Ile Val Gln Gly Leu Phe Gly Thr Gly Leu         
                105                 110                 115             

tgg gtt ctt gcc cac gag tgt ggc cac tct gct ttc tcc gac tct aac      678
Trp Val Leu Ala His Glu Cys Gly His Ser Ala Phe Ser Asp Ser Asn         
            120                 125                 130                 

acc gtc aac aac gtc acc gga tgg gtt ctg cac tcc tcc atg ctg gtc      726
Thr Val Asn Asn Val Thr Gly Trp Val Leu His Ser Ser Met Leu Val         
        135                 140                 145                     

cct tac tac gcc tgg aag ctg acc cac tcc atg cac cac aag tcc act      774
Pro Tyr Tyr Ala Trp Lys Leu Thr His Ser Met His His Lys Ser Thr         
    150                 155                 160                         

ggt cac ctc acc cgt gat atg gtg ttt gtg ccc aag gac cga aag gag      822
Gly His Leu Thr Arg Asp Met Val Phe Val Pro Lys Asp Arg Lys Glu         
165                 170                 175                 180         

ttt atg gag aac cga ggc gcc cat gac tgg tct gag ctt gct gag gac      870
Phe Met Glu Asn Arg Gly Ala His Asp Trp Ser Glu Leu Ala Glu Asp         
                185                 190                 195             

gct ccc ctc atg acc ctc tac ggc ctc atc acc cag cag gtg ttt gga      918
Ala Pro Leu Met Thr Leu Tyr Gly Leu Ile Thr Gln Gln Val Phe Gly         
            200                 205                 210                 

tgg cct ctg tat ctg ctg tct tac gtt acc gga cag aag tac ccc aag      966
Trp Pro Leu Tyr Leu Leu Ser Tyr Val Thr Gly Gln Lys Tyr Pro Lys         
        215                 220                 225                     

ctc aac aaa tgg gct gtc aac cac ttc aac ccc aac gcc ccg ctg ttt     1014
Leu Asn Lys Trp Ala Val Asn His Phe Asn Pro Asn Ala Pro Leu Phe         
    230                 235                 240                         

gag aag aag gac tgg ttc aac atc tgg atc tct aac gtc ggt att ggt     1062
Glu Lys Lys Asp Trp Phe Asn Ile Trp Ile Ser Asn Val Gly Ile Gly         
245                 250                 255                 260         

atc acc atg tcc gtc atc gca tac tcc atc aac cga tgg ggc ctg gct     1110
Ile Thr Met Ser Val Ile Ala Tyr Ser Ile Asn Arg Trp Gly Leu Ala         
                265                 270                 275             

tcc gtc acc ctc tac tac ctg atc ccc tac ctg tgg gtc aac cac tgg     1158
Ser Val Thr Leu Tyr Tyr Leu Ile Pro Tyr Leu Trp Val Asn His Trp         
            280                 285                 290                 

ctc gtg gcc atc acc tac ctg cag cac acc gac ccc act ctg ccc cac     1206
Leu Val Ala Ile Thr Tyr Leu Gln His Thr Asp Pro Thr Leu Pro His         
        295                 300                 305                     

tac cac gcc gac cag tgg aac ttc acc cga gga gcc gcc gcc acc atc     1254
Tyr His Ala Asp Gln Trp Asn Phe Thr Arg Gly Ala Ala Ala Thr Ile         
    310                 315                 320                         

gac cga gag ttt ggc ttc atc ggc tcc ttc tgc ttc cat gac atc atc     1302
Asp Arg Glu Phe Gly Phe Ile Gly Ser Phe Cys Phe His Asp Ile Ile         
325                 330                 335                 340         

gag acc cac gtt ctg cac cac tac gtg tct cga att ccc ttc tac aac     1350
Glu Thr His Val Leu His His Tyr Val Ser Arg Ile Pro Phe Tyr Asn         
                345                 350                 355             

gcc cga atc gcc act gag aag atc aag aag gtc atg ggc aag cac tac     1398
Ala Arg Ile Ala Thr Glu Lys Ile Lys Lys Val Met Gly Lys His Tyr         
            360                 365                 370                 

cga cac gac gac acc aac ttc atc aag tct ctt tac act gtc gcc cga     1446
Arg His Asp Asp Thr Asn Phe Ile Lys Ser Leu Tyr Thr Val Ala Arg         
        375                 380                 385                     

acc tgc cag ttt gtt gaa ggt aag gaa ggc att cag atg ttt aga aac     1494
Thr Cys Gln Phe Val Glu Gly Lys Glu Gly Ile Gln Met Phe Arg Asn         
    390                 395                 400                         

gtc aat gga gtc gga gtt gct cct gac ggc ctg cct tct aaa aag         1539
Val Asn Gly Val Gly Val Ala Pro Asp Gly Leu Pro Ser Lys Lys             
405                 410                 415                             

tagagctaga aatgttattt gattgtgttt taactgaaca gcaccgagcc cgaggctaag   1599

ccaagcgaag ccgaggggtt gtgtagtcca tggacgtaac gagtaggcga tatcaccgca   1659

ctcggcactg cgtgtctgcg ttcatgggcg aagtcacatt acgctgacaa ccgttgtagt   1719

ttccctttag tatcaatact gttacaagta ccggtctcgt actcgtactg atacgaatct   1779

gtgggaagaa gtcaccctta tcagaccttc atactgatgt ttcggatatc aatagaactg   1839

gcatagagcc gttaaagaag tttcacttaa tcactccaac cctcctactt gtagattcaa   1899

gcagatcgat aagatggatt tgatggtcag tgctagc                            1936


<210>  39
<211>  419
<212>  PRT
<213>  Yarrowia lipolytica

<400>  39

Met Asp Ser Thr Thr Gln Thr Asn Thr Gly Thr Gly Lys Val Ala Val 
1               5                   10                  15      


Gln Pro Pro Thr Ala Phe Ile Lys Pro Ile Glu Lys Val Ser Glu Pro 
            20                  25                  30          


Val Tyr Asp Thr Phe Gly Asn Glu Phe Thr Pro Pro Asp Tyr Ser Ile 
        35                  40                  45              


Lys Asp Ile Leu Asp Ala Ile Pro Gln Glu Cys Tyr Lys Arg Ser Tyr 
    50                  55                  60                  


Val Lys Ser Tyr Ser Tyr Val Ala Arg Asp Cys Phe Phe Ile Ala Val 
65                  70                  75                  80  


Phe Ala Tyr Met Ala Tyr Ala Tyr Leu Pro Leu Ile Pro Ser Ala Ser 
                85                  90                  95      


Gly Arg Ala Val Ala Trp Ala Met Tyr Ser Ile Val Gln Gly Leu Phe 
            100                 105                 110         


Gly Thr Gly Leu Trp Val Leu Ala His Glu Cys Gly His Ser Ala Phe 
        115                 120                 125             


Ser Asp Ser Asn Thr Val Asn Asn Val Thr Gly Trp Val Leu His Ser 
    130                 135                 140                 


Ser Met Leu Val Pro Tyr Tyr Ala Trp Lys Leu Thr His Ser Met His 
145                 150                 155                 160 


His Lys Ser Thr Gly His Leu Thr Arg Asp Met Val Phe Val Pro Lys 
                165                 170                 175     


Asp Arg Lys Glu Phe Met Glu Asn Arg Gly Ala His Asp Trp Ser Glu 
            180                 185                 190         


Leu Ala Glu Asp Ala Pro Leu Met Thr Leu Tyr Gly Leu Ile Thr Gln 
        195                 200                 205             


Gln Val Phe Gly Trp Pro Leu Tyr Leu Leu Ser Tyr Val Thr Gly Gln 
    210                 215                 220                 


Lys Tyr Pro Lys Leu Asn Lys Trp Ala Val Asn His Phe Asn Pro Asn 
225                 230                 235                 240 


Ala Pro Leu Phe Glu Lys Lys Asp Trp Phe Asn Ile Trp Ile Ser Asn 
                245                 250                 255     


Val Gly Ile Gly Ile Thr Met Ser Val Ile Ala Tyr Ser Ile Asn Arg 
            260                 265                 270         


Trp Gly Leu Ala Ser Val Thr Leu Tyr Tyr Leu Ile Pro Tyr Leu Trp 
        275                 280                 285             


Val Asn His Trp Leu Val Ala Ile Thr Tyr Leu Gln His Thr Asp Pro 
    290                 295                 300                 


Thr Leu Pro His Tyr His Ala Asp Gln Trp Asn Phe Thr Arg Gly Ala 
305                 310                 315                 320 


Ala Ala Thr Ile Asp Arg Glu Phe Gly Phe Ile Gly Ser Phe Cys Phe 
                325                 330                 335     


His Asp Ile Ile Glu Thr His Val Leu His His Tyr Val Ser Arg Ile 
            340                 345                 350         


Pro Phe Tyr Asn Ala Arg Ile Ala Thr Glu Lys Ile Lys Lys Val Met 
        355                 360                 365             


Gly Lys His Tyr Arg His Asp Asp Thr Asn Phe Ile Lys Ser Leu Tyr 
    370                 375                 380                 


Thr Val Ala Arg Thr Cys Gln Phe Val Glu Gly Lys Glu Gly Ile Gln 
385                 390                 395                 400 


Met Phe Arg Asn Val Asn Gly Val Gly Val Ala Pro Asp Gly Leu Pro 
                405                 410                 415     


Ser Lys Lys 
            


<210>  40
<211>  1272
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutant EgD8S-23 delta-8 desaturase


<220>
<221>  CDS
<222>  (2)..(1270)

<400>  40
c atg gtg aag gct tct cga cag gct ctg ccc ctc gtc atc gac gga aag     49
  Met Val Lys Ala Ser Arg Gln Ala Leu Pro Leu Val Ile Asp Gly Lys       
  1               5                   10                  15            

gtg tac gac gtc tcc gct tgg gtg aac ttc cac cct ggt gga gct gaa       97
Val Tyr Asp Val Ser Ala Trp Val Asn Phe His Pro Gly Gly Ala Glu         
            20                  25                  30                  

atc att gag aac tac cag gga cga gat gct act gac gcc ttc atg gtt      145
Ile Ile Glu Asn Tyr Gln Gly Arg Asp Ala Thr Asp Ala Phe Met Val         
        35                  40                  45                      

atg cac tct cag gaa gcc ttc gac aag ctc aag cga atg ccc aag atc      193
Met His Ser Gln Glu Ala Phe Asp Lys Leu Lys Arg Met Pro Lys Ile         
    50                  55                  60                          

aac cag gct tcc gag ctg cct ccc cag gct gcc gtc aac gaa gct cag      241
Asn Gln Ala Ser Glu Leu Pro Pro Gln Ala Ala Val Asn Glu Ala Gln         
65                  70                  75                  80          

gag gat ttc cga aag ctc cga gaa gag ctg atc gcc act ggc atg ttt      289
Glu Asp Phe Arg Lys Leu Arg Glu Glu Leu Ile Ala Thr Gly Met Phe         
                85                  90                  95              

gac gcc tct ccc ctc tgg tac tcg tac aag atc ttg acc acc ctg ggt      337
Asp Ala Ser Pro Leu Trp Tyr Ser Tyr Lys Ile Leu Thr Thr Leu Gly         
            100                 105                 110                 

ctt ggc gtg ctt gcc ttc ttc atg ctg gtc cag tac cac ctg tac ttc      385
Leu Gly Val Leu Ala Phe Phe Met Leu Val Gln Tyr His Leu Tyr Phe         
        115                 120                 125                     

att ggt gct ctc gtg ctc ggt atg cac tac cag caa atg gga tgg ctg      433
Ile Gly Ala Leu Val Leu Gly Met His Tyr Gln Gln Met Gly Trp Leu         
    130                 135                 140                         

tct cat gac atc tgc cac cac cag acc ttc aag aac cga aac tgg aat      481
Ser His Asp Ile Cys His His Gln Thr Phe Lys Asn Arg Asn Trp Asn         
145                 150                 155                 160         

aac gtc ctg ggt ctg gtc ttt ggc aac gga ctc cag ggc ttc tcc gtg      529
Asn Val Leu Gly Leu Val Phe Gly Asn Gly Leu Gln Gly Phe Ser Val         
                165                 170                 175             

acc tgg tgg aag gac aga cac aac gcc cat cat tct gct acc aac gtt      577
Thr Trp Trp Lys Asp Arg His Asn Ala His His Ser Ala Thr Asn Val         
            180                 185                 190                 

cag ggt cac gat ccc gac att gat aac ctg cct ctg ctc gcc tgg tcc      625
Gln Gly His Asp Pro Asp Ile Asp Asn Leu Pro Leu Leu Ala Trp Ser         
        195                 200                 205                     

gag gac gat gtc act cga gct tct ccc atc tcc cga aag ctc att cag      673
Glu Asp Asp Val Thr Arg Ala Ser Pro Ile Ser Arg Lys Leu Ile Gln         
    210                 215                 220                         

ttc caa cag tac tat ttc ctg gtc atc tgt att ctc ctg cga ttc atc      721
Phe Gln Gln Tyr Tyr Phe Leu Val Ile Cys Ile Leu Leu Arg Phe Ile         
225                 230                 235                 240         

tgg tgt ttc cag tct gtg ctg acc gtt cga tcc ctc aag gac cga gac      769
Trp Cys Phe Gln Ser Val Leu Thr Val Arg Ser Leu Lys Asp Arg Asp         
                245                 250                 255             

aac cag ttc tac cga tct cag tac aag aaa gag gcc att gga ctc gct      817
Asn Gln Phe Tyr Arg Ser Gln Tyr Lys Lys Glu Ala Ile Gly Leu Ala         
            260                 265                 270                 

ctg cac tgg act ctc aag acc ctg ttc cac ctc ttc ttt atg ccc tcc      865
Leu His Trp Thr Leu Lys Thr Leu Phe His Leu Phe Phe Met Pro Ser         
        275                 280                 285                     

atc ctg acc tcg atg ctg gtg ttc ttt gtt tcc gag ctc gtc ggt ggc      913
Ile Leu Thr Ser Met Leu Val Phe Phe Val Ser Glu Leu Val Gly Gly         
    290                 295                 300                         

ttc gga att gcc atc gtg gtc ttc atg aac cac tac cct ctg gag aag      961
Phe Gly Ile Ala Ile Val Val Phe Met Asn His Tyr Pro Leu Glu Lys         
305                 310                 315                 320         

atc ggt gat tcc gtc tgg gac gga cat ggc ttc tct gtg ggt cag atc     1009
Ile Gly Asp Ser Val Trp Asp Gly His Gly Phe Ser Val Gly Gln Ile         
                325                 330                 335             

cat gag acc atg aac att cga cga ggc atc att act gac tgg ttc ttt     1057
His Glu Thr Met Asn Ile Arg Arg Gly Ile Ile Thr Asp Trp Phe Phe         
            340                 345                 350                 

gga ggc ctg aac tac cag atc gag cac cat ctc tgg ccc acc ctg cct     1105
Gly Gly Leu Asn Tyr Gln Ile Glu His His Leu Trp Pro Thr Leu Pro         
        355                 360                 365                     

cga cac aac ctc act gcc gtt tcc tac cag gtg gaa cag ctg tgc cag     1153
Arg His Asn Leu Thr Ala Val Ser Tyr Gln Val Glu Gln Leu Cys Gln         
    370                 375                 380                         

aag cac aac ctc ccc tac cga aac cct ctg ccc cat gaa ggt ctc gtc     1201
Lys His Asn Leu Pro Tyr Arg Asn Pro Leu Pro His Glu Gly Leu Val         
385                 390                 395                 400         

atc ctg ctc cga tac ctg tcc cag ttc gct cga atg gcc gag aag cag     1249
Ile Leu Leu Arg Tyr Leu Ser Gln Phe Ala Arg Met Ala Glu Lys Gln         
                405                 410                 415             

ccc ggt gcc aag gct cag taa gc                                      1272
Pro Gly Ala Lys Ala Gln                                                 
            420                                                         


<210>  41
<211>  422
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  41

Met Val Lys Ala Ser Arg Gln Ala Leu Pro Leu Val Ile Asp Gly Lys 
1               5                   10                  15      


Val Tyr Asp Val Ser Ala Trp Val Asn Phe His Pro Gly Gly Ala Glu 
            20                  25                  30          


Ile Ile Glu Asn Tyr Gln Gly Arg Asp Ala Thr Asp Ala Phe Met Val 
        35                  40                  45              


Met His Ser Gln Glu Ala Phe Asp Lys Leu Lys Arg Met Pro Lys Ile 
    50                  55                  60                  


Asn Gln Ala Ser Glu Leu Pro Pro Gln Ala Ala Val Asn Glu Ala Gln 
65                  70                  75                  80  


Glu Asp Phe Arg Lys Leu Arg Glu Glu Leu Ile Ala Thr Gly Met Phe 
                85                  90                  95      


Asp Ala Ser Pro Leu Trp Tyr Ser Tyr Lys Ile Leu Thr Thr Leu Gly 
            100                 105                 110         


Leu Gly Val Leu Ala Phe Phe Met Leu Val Gln Tyr His Leu Tyr Phe 
        115                 120                 125             


Ile Gly Ala Leu Val Leu Gly Met His Tyr Gln Gln Met Gly Trp Leu 
    130                 135                 140                 


Ser His Asp Ile Cys His His Gln Thr Phe Lys Asn Arg Asn Trp Asn 
145                 150                 155                 160 


Asn Val Leu Gly Leu Val Phe Gly Asn Gly Leu Gln Gly Phe Ser Val 
                165                 170                 175     


Thr Trp Trp Lys Asp Arg His Asn Ala His His Ser Ala Thr Asn Val 
            180                 185                 190         


Gln Gly His Asp Pro Asp Ile Asp Asn Leu Pro Leu Leu Ala Trp Ser 
        195                 200                 205             


Glu Asp Asp Val Thr Arg Ala Ser Pro Ile Ser Arg Lys Leu Ile Gln 
    210                 215                 220                 


Phe Gln Gln Tyr Tyr Phe Leu Val Ile Cys Ile Leu Leu Arg Phe Ile 
225                 230                 235                 240 


Trp Cys Phe Gln Ser Val Leu Thr Val Arg Ser Leu Lys Asp Arg Asp 
                245                 250                 255     


Asn Gln Phe Tyr Arg Ser Gln Tyr Lys Lys Glu Ala Ile Gly Leu Ala 
            260                 265                 270         


Leu His Trp Thr Leu Lys Thr Leu Phe His Leu Phe Phe Met Pro Ser 
        275                 280                 285             


Ile Leu Thr Ser Met Leu Val Phe Phe Val Ser Glu Leu Val Gly Gly 
    290                 295                 300                 


Phe Gly Ile Ala Ile Val Val Phe Met Asn His Tyr Pro Leu Glu Lys 
305                 310                 315                 320 


Ile Gly Asp Ser Val Trp Asp Gly His Gly Phe Ser Val Gly Gln Ile 
                325                 330                 335     


His Glu Thr Met Asn Ile Arg Arg Gly Ile Ile Thr Asp Trp Phe Phe 
            340                 345                 350         


Gly Gly Leu Asn Tyr Gln Ile Glu His His Leu Trp Pro Thr Leu Pro 
        355                 360                 365             


Arg His Asn Leu Thr Ala Val Ser Tyr Gln Val Glu Gln Leu Cys Gln 
    370                 375                 380                 


Lys His Asn Leu Pro Tyr Arg Asn Pro Leu Pro His Glu Gly Leu Val 
385                 390                 395                 400 


Ile Leu Leu Arg Tyr Leu Ser Gln Phe Ala Arg Met Ala Glu Lys Gln 
                405                 410                 415     


Pro Gly Ala Lys Ala Gln 
            420         


<210>  42
<211>  777
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(777)
<223>  delta-9 elongase

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  WO 2007/061742
<311>  2006-11-16
<312>  2007-05-31
<313>  (1)..(777)

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  US 2007-0117190-A1
<311>  2006-11-16
<312>  2007-05-24
<313>  (1)..(777)

<400>  42
atg gag gtg gtg aat gaa ata gtc tca att ggg cag gaa gtt tta ccc       48
Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro         
1               5                   10                  15              

aaa gtt gat tat gcc caa ctc tgg agt gat gcc agt cac tgt gag gtg       96
Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val         
            20                  25                  30                  

ctt tac ttg tcc atc gca ttt gtc atc ttg aag ttc act ctt ggc ccc      144
Leu Tyr Leu Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro         
        35                  40                  45                      

ctt ggt cca aaa ggt cag tct cgt atg aag ttt gtt ttc acc aat tac      192
Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr         
    50                  55                  60                          

aac ctt ctc atg tcc att tat tcg ttg gga tca ttc ctc tca atg gca      240
Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala         
65                  70                  75                  80          

tat gcc atg tac acc atc ggt gtt atg tct gac aac tgc gag aag gct      288
Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala         
                85                  90                  95              

ttt gac aac aac gtc ttc agg atc acc acg cag ttg ttc tat ttg agc      336
Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser         
            100                 105                 110                 

aag ttc ctg gag tat att gac tcc ttc tat ttg cca ctg atg ggc aag      384
Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys         
        115                 120                 125                     

cct ctg acc tgg ttg caa ttc ttc cat cat ttg ggg gca ccg atg gat      432
Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp         
    130                 135                 140                         

atg tgg ctg ttc tat aat tac cga aat gaa gct gtt tgg att ttt gtg      480
Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val         
145                 150                 155                 160         

ctg ttg aat ggt ttc atc cac tgg atc atg tac ggt tat tat tgg acc      528
Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr         
                165                 170                 175             

aga ttg atc aag ctg aag ttc ccc atg cca aaa tcc ctg att aca tca      576
Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser         
            180                 185                 190                 

atg cag atc att caa ttc aat gtt ggt ttc tac att gtc tgg aag tac      624
Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr         
        195                 200                 205                     

agg aac att ccc tgt tat cgc caa gat ggg atg agg atg ttt ggc tgg      672
Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp         
    210                 215                 220                         

ttc ttc aat tac ttt tat gtt ggc aca gtc ttg tgt ttg ttc ttg aat      720
Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn         
225                 230                 235                 240         

ttc tat gtg caa acg tat atc gtc agg aag cac aag gga gcc aaa aag      768
Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys         
                245                 250                 255             

att cag tga                                                          777
Ile Gln                                                                 
                                                                        


<210>  43
<211>  258
<212>  PRT
<213>  Euglena gracilis

<400>  43

Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro 
1               5                   10                  15      


Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val 
            20                  25                  30          


Leu Tyr Leu Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro 
        35                  40                  45              


Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr 
    50                  55                  60                  


Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala 
65                  70                  75                  80  


Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala 
                85                  90                  95      


Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser 
            100                 105                 110         


Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys 
        115                 120                 125             


Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp 
    130                 135                 140                 


Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val 
145                 150                 155                 160 


Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr 
                165                 170                 175     


Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 
        195                 200                 205             


Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp 
    210                 215                 220                 


Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys 
                245                 250                 255     


Ile Gln 
        


<210>  44
<211>  8357
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZUFmEaD5S

<400>  44
catggccacc atctccctga ctaccgagca gctcctggaa caccccgagc tcgttgccat     60

cgacggagtc ctgtacgatc tcttcggtct ggccaaggtg catccaggag gcaacctcat    120

cgaagctgcc ggtgcatccg acggaaccgc tctgttctac tccatgcatc ctggagtcaa    180

gccagagaac tcgaagcttc tgcagcaatt tgcccgaggc aagcacgaac gaagctccaa    240

ggatcccgtg tacaccttcg actctccctt tgctcaggac gtcaagcagt ccgttcgaga    300

ggtcatgaag ggtcgaaact ggtacgccac tcctggcttc tggctgagaa ccgcactcat    360

catcgcttgt actgccattg gcgagtggta ctggatcaca accggagcag tgatgtgggg    420

tatctttact ggatacttcc actcgcagat tggcttggcc attcaacacg atgcttctca    480

cggagccatc agcaaaaagc cctgggtcaa cgcctttttc gcttatggca tcgacgccat    540

tggttcctct cgttggatct ggctgcagtc ccacattatg cgacatcaca cttacaccaa    600

ccagcatggc ctcgacctgg atgctgcctc ggcagagccg ttcatcttgt tccactccta    660

tcctgctacc aacgcctctc gaaagtggta ccaccgattt caggcgtggt acatgtacat    720

cgttctggga atgtatggtg tctcgatggt gtacaatccc atgtacctct tcacaatgca    780

gcacaacgac accattcccg aggccacttc tctcagacca ggcagctttt tcaatcggca    840

gcgagctttc gccgtttccc ttcgactgct cttcatcttc cgaaacgcct ttcttccctg    900

gtacattgct ggtgcctctc ctctgctcac cattcttctg gtgcccacgg tcacaggcat    960

cttcctcacc tttgtgttcg ttctgtccca taacttcgag ggagccgaac ggaccccaga   1020

gaagaactgc aaggccaaac gagctaagga aggcaaggag gtcagagacg tggaagagga   1080

tcgagtcgac tggtaccgag cacaggccga gactgctgcc acctacggtg gcagcgtggg   1140

aatgatgctt acaggcggtc tcaacctgca gatcgagcat cacttgtttc cccgaatgtc   1200

ctcttggcac tatcccttca ttcaagacac cgttcgggag tgttgcaagc gacatggcgt   1260

ccgttacaca tactatccta ccattctcga gaacatcatg tccactcttc gatacatgca   1320

gaaggtgggt gttgctcaca ccattcagga tgcccaggag ttctaagcgg ccgcaagtgt   1380

ggatggggaa gtgagtgccc ggttctgtgt gcacaattgg caatccaaga tggatggatt   1440

caacacaggg atatagcgag ctacgtggtg gtgcgaggat atagcaacgg atatttatgt   1500

ttgacacttg agaatgtacg atacaagcac tgtccaagta caatactaaa catactgtac   1560

atactcatac tcgtacccgg gcaacggttt cacttgagtg cagtggctag tgctcttact   1620

cgtacagtgt gcaatactgc gtatcatagt ctttgatgta tatcgtattc attcatgtta   1680

gttgcgtacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac   1740

tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc   1800

tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg   1860

cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc   1920

actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt   1980

gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc   2040

ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa   2100

acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc   2160

ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg   2220

cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc   2280

tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc   2340

gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca   2400

ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact   2460

acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg   2520

gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt   2580

ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct   2640

tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga   2700

gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa   2760

tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac   2820

ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga   2880

taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc   2940

cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca   3000

gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta   3060

gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg   3120

tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc   3180

gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg   3240

ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt   3300

ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt   3360

cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata   3420

ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc   3480

gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac   3540

ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa   3600

ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct   3660

tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat   3720

ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc   3780

cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg   3840

tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc   3900

tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc   3960

gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta   4020

gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta   4080

atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg   4140

atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa   4200

aatttaacgc gaattttaac aaaatattaa cgcttacaat ttccattcgc cattcaggct   4260

gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa   4320

agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg   4380

ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tgggtaccgg   4440

gccccccctc gaggtcgatg gtgtcgataa gcttgatatc gaattcatgt cacacaaacc   4500

gatcttcgcc tcaaggaaac ctaattctac atccgagaga ctgccgagat ccagtctaca   4560

ctgattaatt ttcgggccaa taatttaaaa aaatcgtgtt atataatatt atatgtatta   4620

tatatataca tcatgatgat actgacagtc atgtcccatt gctaaataga cagactccat   4680

ctgccgcctc caactgatgt tctcaatatt taaggggtca tctcgcattg tttaataata   4740

aacagactcc atctaccgcc tccaaatgat gttctcaaaa tatattgtat gaacttattt   4800

ttattactta gtattattag acaacttact tgctttatga aaaacacttc ctatttagga   4860

aacaatttat aatggcagtt cgttcattta acaatttatg tagaataaat gttataaatg   4920

cgtatgggaa atcttaaata tggatagcat aaatgatatc tgcattgcct aattcgaaat   4980

caacagcaac gaaaaaaatc ccttgtacaa cataaatagt catcgagaaa tatcaactat   5040

caaagaacag ctattcacac gttactattg agattattat tggacgagaa tcacacactc   5100

aactgtcttt ctctcttcta gaaatacagg tacaagtatg tactattctc attgttcata   5160

cttctagtca tttcatccca catattcctt ggatttctct ccaatgaatg acattctatc   5220

ttgcaaattc aacaattata ataagatata ccaaagtagc ggtatagtgg caatcaaaaa   5280

gcttctctgg tgtgcttctc gtatttattt ttattctaat gatccattaa aggtatatat   5340

ttatttcttg ttatataatc cttttgttta ttacatgggc tggatacata aaggtatttt   5400

gatttaattt tttgcttaaa ttcaatcccc cctcgttcag tgtcaactgt aatggtagga   5460

aattaccata cttttgaaga agcaaaaaaa atgaaagaaa aaaaaaatcg tatttccagg   5520

ttagacgttc cgcagaatct agaatgcggt atgcggtaca ttgttcttcg aacgtaaaag   5580

ttgcgctccc tgagatattg tacatttttg cttttacaag tacaagtaca tcgtacaact   5640

atgtactact gttgatgcat ccacaacagt ttgttttgtt tttttttgtt tttttttttt   5700

ctaatgattc attaccgcta tgtataccta cttgtacttg tagtaagccg ggttattggc   5760

gttcaattaa tcatagactt atgaatctgc acggtgtgcg ctgcgagtta cttttagctt   5820

atgcatgcta cttgggtgta atattgggat ctgttcggaa atcaacggat gctcaatcga   5880

tttcgacagt aattaattaa gtcatacaca agtcagcttt cttcgagcct catataagta   5940

taagtagttc aacgtattag cactgtaccc agcatctccg tatcgagaaa cacaacaaca   6000

tgccccattg gacagatcat gcggatacac aggttgtgca gtatcataca tactcgatca   6060

gacaggtcgt ctgaccatca tacaagctga acaagcgctc catacttgca cgctctctat   6120

atacacagtt aaattacata tccatagtct aacctctaac agttaatctt ctggtaagcc   6180

tcccagccag ccttctggta tcgcttggcc tcctcaatag gatctcggtt ctggccgtac   6240

agacctcggc cgacaattat gatatccgtt ccggtagaca tgacatcctc aacagttcgg   6300

tactgctgtc cgagagcgtc tcccttgtcg tcaagaccca ccccgggggt cagaataagc   6360

cagtcctcag agtcgccctt aggtcggttc tgggcaatga agccaaccac aaactcgggg   6420

tcggatcggg caagctcaat ggtctgcttg gagtactcgc cagtggccag agagcccttg   6480

caagacagct cggccagcat gagcagacct ctggccagct tctcgttggg agaggggact   6540

aggaactcct tgtactggga gttctcgtag tcagagacgt cctccttctt ctgttcagag   6600

acagtttcct cggcaccagc tcgcaggcca gcaatgattc cggttccggg tacaccgtgg   6660

gcgttggtga tatcggacca ctcggcgatt cggtgacacc ggtactggtg cttgacagtg   6720

ttgccaatat ctgcgaactt tctgtcctcg aacaggaaga aaccgtgctt aagagcaagt   6780

tccttgaggg ggagcacagt gccggcgtag gtgaagtcgt caatgatgtc gatatgggtt   6840

ttgatcatgc acacataagg tccgacctta tcggcaagct caatgagctc cttggtggtg   6900

gtaacatcca gagaagcaca caggttggtt ttcttggctg ccacgagctt gagcactcga   6960

gcggcaaagg cggacttgtg gacgttagct cgagcttcgt aggagggcat tttggtggtg   7020

aagaggagac tgaaataaat ttagtctgca gaacttttta tcggaacctt atctggggca   7080

gtgaagtata tgttatggta atagttacga gttagttgaa cttatagata gactggacta   7140

tacggctatc ggtccaaatt agaaagaacg tcaatggctc tctgggcgtc gcctttgccg   7200

acaaaaatgt gatcatgatg aaagccagca atgacgttgc agctgatatt gttgtcggcc   7260

aaccgcgccg aaaacgcagc tgtcagaccc acagcctcca acgaagaatg tatcgtcaaa   7320

gtgatccaag cacactcata gttggagtcg tactccaaag gcggcaatga cgagtcagac   7380

agatactcgt cgacgtttaa acagtgtacg cagatctact atagaggaac atttaaattg   7440

ccccggagaa gacggccagg ccgcctagat gacaaattca acaactcaca gctgactttc   7500

tgccattgcc actagggggg ggccttttta tatggccaag ccaagctctc cacgtcggtt   7560

gggctgcacc caacaataaa tgggtagggt tgcaccaaca aagggatggg atggggggta   7620

gaagatacga ggataacggg gctcaatggc acaaataaga acgaatactg ccattaagac   7680

tcgtgatcca gcgactgaca ccattgcatc atctaagggc ctcaaaacta cctcggaact   7740

gctgcgctga tctggacacc acagaggttc cgagcacttt aggttgcacc aaatgtccca   7800

ccaggtgcag gcagaaaacg ctggaacagc gtgtacagtt tgtcttaaca aaaagtgagg   7860

gcgctgaggt cgagcagggt ggtgtgactt gttatagcct ttagagctgc gaaagcgcgt   7920

atggatttgg ctcatcaggc cagattgagg gtctgtggac acatgtcatg ttagtgtact   7980

tcaatcgccc cctggatata gccccgacaa taggccgtgg cctcattttt ttgccttccg   8040

cacatttcca ttgctcgata cccacacctt gcttctcctg cacttgccaa ccttaatact   8100

ggtttacatt gaccaacatc ttacaagcgg ggggcttgtc tagggtatat ataaacagtg   8160

gctctcccaa tcggttgcca gtctcttttt tcctttcttt ccccacagat tcgaaatcta   8220

aactacacat cacagaattc cgagccgtga gtatccacga caagatcagt gtcgagacga   8280

cgcgttttgt gtaatgacac aatccgaaag tcgctagcaa cacacactct ctacacaaac   8340

taacccagct ctggtac                                                  8357


