                         SEQUENCE LISTING

<110>  Kraynov, Vadim
       Knudsen, Nick
       Hays Putnam, Anna-Maria A.
       Krawitz, Denise
       Pinkstaff, Jason
       Myler, Heather
 
<120>  Modified Interferon Beta Polypeptides and Their Uses

<130>  AMBX-0136.00PCT

<150>  60/927,528
<151>  2007-05-02

<160>  24    

<170>  PatentIn version 3.4

<210>  1
<211>  166
<212>  PRT
<213>  Artificial

<220>
<223>  Amino acid sequence of IFN beta with C17S substitution

<400>  1

Met Ser Tyr Asn Leu Leu Gly Phe Leu Gln Arg Ser Ser Asn Phe Gln 
1               5                   10                  15      


Ser Gln Lys Leu Leu Trp Gln Leu Asn Gly Arg Leu Glu Tyr Cys Leu 
            20                  25                  30          


Lys Asp Arg Met Asn Phe Asp Ile Pro Glu Glu Ile Lys Gln Leu Gln 
        35                  40                  45              


Gln Phe Gln Lys Glu Asp Ala Ala Leu Thr Ile Tyr Glu Met Leu Gln 
    50                  55                  60                  


Asn Ile Phe Ala Ile Phe Arg Gln Asp Ser Ser Ser Thr Gly Trp Asn 
65                  70                  75                  80  


Glu Thr Ile Val Glu Asn Leu Leu Ala Asn Val Tyr His Gln Ile Asn 
                85                  90                  95      


His Leu Lys Thr Val Leu Glu Glu Lys Leu Glu Lys Glu Asp Phe Thr 
            100                 105                 110         


Arg Gly Lys Leu Met Ser Ser Leu His Leu Lys Arg Tyr Tyr Gly Arg 
        115                 120                 125             


Ile Leu His Tyr Leu Lys Ala Lys Glu Tyr Ser His Cys Ala Trp Thr 
    130                 135                 140                 


Ile Val Arg Val Glu Ile Leu Arg Asn Phe Tyr Phe Ile Asn Arg Leu 
145                 150                 155                 160 


Thr Gly Tyr Leu Arg Asn 
                165     


<210>  2
<211>  498
<212>  DNA
<213>  Artificial

<220>
<223>  Nucleotide sequence of IFN beta with C17S substitution (optimized
       5' end)

<400>  2
atgagctaca acttgcttgg attcctgcag cgttcttcta actttcagag ccagaaactg     60

ctgtggcagc ttaacggtcg tctggagtac tgccttaaag accgtatgaa cttcgacatc    120

ccggaagaaa tcaaacagct ccagcagttc caaaaagaag acgcggccct gaccatctac    180

gaaatgctgc agaacatctt cgcgatcttt cgtcaggata gctctagcac cggttggaat    240

gaaaccatcg ttgaaaacct gcttgctaac gtttaccacc agattaacca ccttaaaacc    300

gttcttgaag aaaaactgga gaaagaagac ttcacccgtg gtaaactgat gtctagcctg    360

cacctgaagc gttactacgg tcgtatcctg cactacctta aagccaaaga atattctcac    420

tgcgcgtgga ccatcgttcg tgttgaaatc ctgcgtaact tctacttcat caaccgtctg    480

accggctacc tgcgcaac                                                  498


<210>  3
<211>  166
<212>  PRT
<213>  Homo sapiens

<400>  3

Met Ser Tyr Asn Leu Leu Gly Phe Leu Gln Arg Ser Ser Asn Phe Gln 
1               5                   10                  15      


Cys Gln Lys Leu Leu Trp Gln Leu Asn Gly Arg Leu Glu Tyr Cys Leu 
            20                  25                  30          


Lys Asp Arg Met Asn Phe Asp Ile Pro Glu Glu Ile Lys Gln Leu Gln 
        35                  40                  45              


Gln Phe Gln Lys Glu Asp Ala Ala Leu Thr Ile Tyr Glu Met Leu Gln 
    50                  55                  60                  


Asn Ile Phe Ala Ile Phe Arg Gln Asp Ser Ser Ser Thr Gly Trp Asn 
65                  70                  75                  80  


Glu Thr Ile Val Glu Asn Leu Leu Ala Asn Val Tyr His Gln Ile Asn 
                85                  90                  95      


His Leu Lys Thr Val Leu Glu Glu Lys Leu Glu Lys Glu Asp Phe Thr 
            100                 105                 110         


Arg Gly Lys Leu Met Ser Ser Leu His Leu Lys Arg Tyr Tyr Gly Arg 
        115                 120                 125             


Ile Leu His Tyr Leu Lys Ala Lys Glu Tyr Ser His Cys Ala Trp Thr 
    130                 135                 140                 


Ile Val Arg Val Glu Ile Leu Arg Asn Phe Tyr Phe Ile Asn Arg Leu 
145                 150                 155                 160 


Thr Gly Tyr Leu Arg Asn 
                165     


<210>  4
<211>  187
<212>  PRT
<213>  Homo sapiens

<400>  4

Met Thr Asn Lys Cys Leu Leu Gln Ile Ala Leu Leu Leu Cys Phe Ser 
1               5                   10                  15      


Thr Thr Ala Leu Ser Met Ser Tyr Asn Leu Leu Gly Phe Leu Gln Arg 
            20                  25                  30          


Ser Ser Asn Phe Gln Cys Gln Lys Leu Leu Trp Gln Leu Asn Gly Arg 
        35                  40                  45              


Leu Glu Tyr Cys Leu Lys Asp Arg Met Asn Phe Asp Ile Pro Glu Glu 
    50                  55                  60                  


Ile Lys Gln Leu Gln Gln Phe Gln Lys Glu Asp Ala Ala Leu Thr Ile 
65                  70                  75                  80  


Tyr Glu Met Leu Gln Asn Ile Phe Ala Ile Phe Arg Gln Asp Ser Ser 
                85                  90                  95      


Ser Thr Gly Trp Asn Glu Thr Ile Val Glu Asn Leu Leu Ala Asn Val 
            100                 105                 110         


Tyr His Gln Ile Asn His Leu Lys Thr Val Leu Glu Glu Lys Leu Glu 
        115                 120                 125             


Lys Glu Asp Phe Thr Arg Gly Lys Leu Met Ser Ser Leu His Leu Lys 
    130                 135                 140                 


Arg Tyr Tyr Gly Arg Ile Leu His Tyr Leu Lys Ala Lys Glu Tyr Ser 
145                 150                 155                 160 


His Cys Ala Trp Thr Ile Val Arg Val Glu Ile Leu Arg Asn Phe Tyr 
                165                 170                 175     


Phe Ile Asn Arg Leu Thr Gly Tyr Leu Arg Asn 
            180                 185         


<210>  5
<211>  77
<212>  DNA
<213>  Artificial

<220>
<223>  Mutant tRNA derived from Methanococcus jannaschii tRNA

<400>  5
ccggcggtag ttcagcaggg cagaacggcg gactctaaat ccgcatggcg ctggttcaaa     60

tccagcccgc cggacca                                                    77


<210>  6
<211>  306
<212>  PRT
<213>  Artificial

<220>
<223>  Mutant synthetase derived from Methanococcus jannaschii 
       synthetase

<400>  6

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Val 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Tyr Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Glu His Gly Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Gly Ile His 
145                 150                 155                 160 


Tyr Glu Gly Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile 
                165                 170                 175     


His Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His 
            180                 185                 190         


Asn Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser 
        195                 200                 205             


Lys Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala 
    210                 215                 220                 


Lys Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro 
225                 230                 235                 240 


Ile Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys 
                245                 250                 255     


Arg Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu 
            260                 265                 270         


Leu Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys 
        275                 280                 285             


Asn Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys 
    290                 295                 300                 


Arg Leu 
305     


<210>  7
<211>  77
<212>  DNA
<213>  Methanococcus jannaschii

<400>  7
ccggcggtag ttcagcaggg cagaacggcg gactctaaat ccgcatggcg ctggttcaaa     60

tccggcccgc cggacca                                                    77


<210>  8
<211>  88
<212>  DNA
<213>  Artificial

<220>
<223>  Optimized amber supressor tRNA

<400>  8
cccagggtag ccaagctcgg ccaacggcga cggactctaa atccgttctc gtaggagttc     60

gagggttcga atcccttccc tgggacca                                        88


<210>  9
<211>  89
<212>  DNA
<213>  Artificial

<220>
<223>  Optimized AGGA frameshift supressor tRNA

<400>  9
gcgagggtag ccaagctcgg ccaacggcga cggacttcct aatccgttct cgtaggagtt     60

cgagggttcg aatccctccc ctcgcacca                                       89


<210>  10
<211>  306
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase  for the incorporation of 
       p-azido-L-phenylalanine

<400>  10

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Gly 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Thr Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Thr Tyr Tyr 
145                 150                 155                 160 


Tyr Leu Gly Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile 
                165                 170                 175     


His Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His 
            180                 185                 190         


Asn Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser 
        195                 200                 205             


Lys Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala 
    210                 215                 220                 


Lys Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro 
225                 230                 235                 240 


Ile Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys 
                245                 250                 255     


Arg Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu 
            260                 265                 270         


Leu Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys 
        275                 280                 285             


Asn Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys 
    290                 295                 300                 


Arg Leu 
305     


<210>  11
<211>  306
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase  for the incorporation of 
       p-benzoyl-L-phenylalanine

<400>  11

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Gly 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Ser Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Thr Ser His 
145                 150                 155                 160 


Tyr Leu Gly Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile 
                165                 170                 175     


His Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His 
            180                 185                 190         


Asn Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser 
        195                 200                 205             


Lys Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala 
    210                 215                 220                 


Lys Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro 
225                 230                 235                 240 


Ile Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys 
                245                 250                 255     


Arg Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu 
            260                 265                 270         


Leu Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys 
        275                 280                 285             


Asn Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys 
    290                 295                 300                 


Arg Leu 
305     


<210>  12
<211>  305
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase for the incorporation of 
       propargyl-phenylalanine

<400>  12

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Ala 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Pro Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Ala Ile Tyr 
145                 150                 155                 160 


Leu Ala Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile His 
                165                 170                 175     


Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His Asn 
            180                 185                 190         


Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser Lys 
        195                 200                 205             


Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala Lys 
    210                 215                 220                 


Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro Ile 
225                 230                 235                 240 


Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys Arg 
                245                 250                 255     


Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu Leu 
            260                 265                 270         


Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys Asn 
        275                 280                 285             


Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys Arg 
    290                 295                 300                 


Leu 
305 


<210>  13
<211>  305
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase for the incorporation of 
       propargyl-phenylalanine

<400>  13

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Ala 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Pro Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Ile Pro Tyr 
145                 150                 155                 160 


Leu Pro Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile His 
                165                 170                 175     


Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His Asn 
            180                 185                 190         


Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser Lys 
        195                 200                 205             


Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala Lys 
    210                 215                 220                 


Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro Ile 
225                 230                 235                 240 


Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys Arg 
                245                 250                 255     


Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu Leu 
            260                 265                 270         


Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys Asn 
        275                 280                 285             


Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys Arg 
    290                 295                 300                 


Leu 
305 


<210>  14
<211>  305
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase for the incorporation of 
       propargyl-phenylalanine

<400>  14

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Ala 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Lys Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Ala Ile Tyr 
145                 150                 155                 160 


Leu Ala Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile His 
                165                 170                 175     


Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His Asn 
            180                 185                 190         


Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser Lys 
        195                 200                 205             


Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala Lys 
    210                 215                 220                 


Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro Ile 
225                 230                 235                 240 


Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys Arg 
                245                 250                 255     


Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu Leu 
            260                 265                 270         


Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys Asn 
        275                 280                 285             


Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys Arg 
    290                 295                 300                 


Leu 
305 


<210>  15
<211>  306
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase  for the incorporation of 
       p-azido-phenylalanine

<400>  15

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Thr 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Asn Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Pro Leu His 
145                 150                 155                 160 


Tyr Gln Gly Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile 
                165                 170                 175     


His Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His 
            180                 185                 190         


Asn Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser 
        195                 200                 205             


Lys Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala 
    210                 215                 220                 


Lys Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro 
225                 230                 235                 240 


Ile Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys 
                245                 250                 255     


Arg Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu 
            260                 265                 270         


Leu Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys 
        275                 280                 285             


Asn Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys 
    290                 295                 300                 


Arg Leu 
305     


<210>  16
<211>  306
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase  for the incorporation of 
       p-azido-phenylalanine

<400>  16

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Thr 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Ser Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Pro Leu His 
145                 150                 155                 160 


Tyr Gln Gly Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile 
                165                 170                 175     


His Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His 
            180                 185                 190         


Asn Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser 
        195                 200                 205             


Lys Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala 
    210                 215                 220                 


Lys Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro 
225                 230                 235                 240 


Ile Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys 
                245                 250                 255     


Arg Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu 
            260                 265                 270         


Leu Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys 
        275                 280                 285             


Asn Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys 
    290                 295                 300                 


Arg Leu 
305     


<210>  17
<211>  306
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase for the incorporation of 
       p-azido-phenylalanine

<400>  17

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Leu 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Thr Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Pro Val His 
145                 150                 155                 160 


Tyr Gln Gly Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile 
                165                 170                 175     


His Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His 
            180                 185                 190         


Asn Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser 
        195                 200                 205             


Lys Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala 
    210                 215                 220                 


Lys Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro 
225                 230                 235                 240 


Ile Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys 
                245                 250                 255     


Arg Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu 
            260                 265                 270         


Leu Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys 
        275                 280                 285             


Asn Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys 
    290                 295                 300                 


Arg Leu 
305     


<210>  18
<211>  306
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase  for the incorporation of 
       p-azido-phenylalanine

<400>  18

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Thr 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Ser Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Pro Ser His 
145                 150                 155                 160 


Tyr Gln Gly Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile 
                165                 170                 175     


His Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His 
            180                 185                 190         


Asn Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser 
        195                 200                 205             


Lys Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala 
    210                 215                 220                 


Lys Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro 
225                 230                 235                 240 


Ile Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys 
                245                 250                 255     


Arg Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu 
            260                 265                 270         


Leu Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys 
        275                 280                 285             


Asn Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys 
    290                 295                 300                 


Arg Leu 
305     


<210>  19
<211>  306
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase  for the incorporation of 
       p-acetyl-phenylalanine

<400>  19

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Leu 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Glu Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Gly Cys His 
145                 150                 155                 160 


Tyr Arg Gly Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile 
                165                 170                 175     


His Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His 
            180                 185                 190         


Asn Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser 
        195                 200                 205             


Lys Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala 
    210                 215                 220                 


Lys Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro 
225                 230                 235                 240 


Ile Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys 
                245                 250                 255     


Arg Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu 
            260                 265                 270         


Leu Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys 
        275                 280                 285             


Asn Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys 
    290                 295                 300                 


Arg Leu 
305     


<210>  20
<211>  306
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase  for the incorporation of p-acetyl 
       -phenylalanine

<400>  20

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Leu 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Glu Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Gly Thr His 
145                 150                 155                 160 


Tyr Arg Gly Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile 
                165                 170                 175     


His Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His 
            180                 185                 190         


Asn Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser 
        195                 200                 205             


Lys Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala 
    210                 215                 220                 


Lys Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro 
225                 230                 235                 240 


Ile Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys 
                245                 250                 255     


Arg Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu 
            260                 265                 270         


Leu Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys 
        275                 280                 285             


Asn Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys 
    290                 295                 300                 


Arg Leu 
305     


<210>  21
<211>  306
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase  for the incorporation of 
       p-acetyl-phenylalanine

<400>  21

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Ala 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Glu Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Gly Gly His 
145                 150                 155                 160 


Tyr Leu Gly Val Asp Val Ile Val Gly Gly Met Glu Gln Arg Lys Ile 
                165                 170                 175     


His Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His 
            180                 185                 190         


Asn Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser 
        195                 200                 205             


Lys Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala 
    210                 215                 220                 


Lys Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro 
225                 230                 235                 240 


Ile Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys 
                245                 250                 255     


Arg Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu 
            260                 265                 270         


Leu Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys 
        275                 280                 285             


Asn Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys 
    290                 295                 300                 


Arg Leu 
305     


<210>  22
<211>  306
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase  for the incorporation of 
       p-azido-phenylalanine

<400>  22

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Ala 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Arg Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Val Ile His 
145                 150                 155                 160 


Tyr Asp Gly Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile 
                165                 170                 175     


His Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His 
            180                 185                 190         


Asn Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser 
        195                 200                 205             


Lys Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala 
    210                 215                 220                 


Lys Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro 
225                 230                 235                 240 


Ile Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys 
                245                 250                 255     


Arg Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu 
            260                 265                 270         


Leu Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys 
        275                 280                 285             


Asn Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys 
    290                 295                 300                 


Arg Leu 
305     


<210>  23
<211>  306
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase  for the incorporation of 
       p-azido-phenylalanine

<400>  23

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Gly 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Thr Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Thr Tyr Tyr 
145                 150                 155                 160 


Tyr Leu Gly Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile 
                165                 170                 175     


His Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His 
            180                 185                 190         


Asn Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser 
        195                 200                 205             


Lys Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala 
    210                 215                 220                 


Lys Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro 
225                 230                 235                 240 


Ile Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys 
                245                 250                 255     


Arg Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu 
            260                 265                 270         


Leu Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys 
        275                 280                 285             


Asn Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys 
    290                 295                 300                 


Arg Leu 
305     


<210>  24
<211>  306
<212>  PRT
<213>  Artificial

<220>
<223>  Mutant synthetase derived from Methanococcus jannaschii 
       synthetase

<400>  24

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Val 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Tyr Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Glu His Gly Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Gly Ile His 
145                 150                 155                 160 


Tyr Glu Gly Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile 
                165                 170                 175     


His Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His 
            180                 185                 190         


Asn Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser 
        195                 200                 205             


Lys Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala 
    210                 215                 220                 


Lys Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro 
225                 230                 235                 240 


Ile Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys 
                245                 250                 255     


Arg Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu 
            260                 265                 270         


Leu Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Arg Leu Lys 
        275                 280                 285             


Asn Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys 
    290                 295                 300                 


Arg Leu 
305     


