                         SEQUENCE LISTING

<110>  E.I. du Pont de Nemours and Co.
       
       
 
<120>  Method for the Production of 1-Butanol

<130>  CL3674

<160>  208   

<170>  PatentIn version 3.4

<210>  1
<211>  1179
<212>  DNA
<213>  Clostridium acetobutylicum

<400>  1
atgaaagaag ttgtaatagc tagtgcagta agaacagcga ttggatctta tggaaagtct     60

cttaaggatg taccagcagt agatttagga gctacagcta taaaggaagc agttaaaaaa    120

gcaggaataa aaccagagga tgttaatgaa gtcattttag gaaatgttct tcaagcaggt    180

ttaggacaga atccagcaag acaggcatct tttaaagcag gattaccagt tgaaattcca    240

gctatgacta ttaataaggt ttgtggttca ggacttagaa cagttagctt agcagcacaa    300

attataaaag caggagatgc tgacgtaata atagcaggtg gtatggaaaa tatgtctaga    360

gctccttact tagcgaataa cgctagatgg ggatatagaa tgggaaacgc taaatttgtt    420

gatgaaatga tcactgacgg attgtgggat gcatttaatg attaccacat gggaataaca    480

gcagaaaaca tagctgagag atggaacatt tcaagagaag aacaagatga gtttgctctt    540

gcatcacaaa aaaaagctga agaagctata aaatcaggtc aatttaaaga tgaaatagtt    600

cctgtagtaa ttaaaggcag aaagggagaa actgtagttg atacagatga gcaccctaga    660

tttggatcaa ctatagaagg acttgcaaaa ttaaaacctg ccttcaaaaa agatggaaca    720

gttacagctg gtaatgcatc aggattaaat gactgtgcag cagtacttgt aatcatgagt    780

gcagaaaaag ctaaagagct tggagtaaaa ccacttgcta agatagtttc ttatggttca    840

gcaggagttg acccagcaat aatgggatat ggacctttct atgcaacaaa agcagctatt    900

gaaaaagcag gttggacagt tgatgaatta gatttaatag aatcaaatga agcttttgca    960

gctcaaagtt tagcagtagc aaaagattta aaatttgata tgaataaagt aaatgtaaat   1020

ggaggagcta ttgcccttgg tcatccaatt ggagcatcag gtgcaagaat actcgttact   1080

cttgtacacg caatgcaaaa aagagatgca aaaaaaggct tagcaacttt atgtataggt   1140

ggcggacaag gaacagcaat attgctagaa aagtgctag                          1179


<210>  2
<211>  392
<212>  PRT
<213>  Clostridium acetobutylicum

<400>  2

Met Lys Glu Val Val Ile Ala Ser Ala Val Arg Thr Ala Ile Gly Ser 
1               5                   10                  15      


Tyr Gly Lys Ser Leu Lys Asp Val Pro Ala Val Asp Leu Gly Ala Thr 
            20                  25                  30          


Ala Ile Lys Glu Ala Val Lys Lys Ala Gly Ile Lys Pro Glu Asp Val 
        35                  40                  45              


Asn Glu Val Ile Leu Gly Asn Val Leu Gln Ala Gly Leu Gly Gln Asn 
    50                  55                  60                  


Pro Ala Arg Gln Ala Ser Phe Lys Ala Gly Leu Pro Val Glu Ile Pro 
65                  70                  75                  80  


Ala Met Thr Ile Asn Lys Val Cys Gly Ser Gly Leu Arg Thr Val Ser 
                85                  90                  95      


Leu Ala Ala Gln Ile Ile Lys Ala Gly Asp Ala Asp Val Ile Ile Ala 
            100                 105                 110         


Gly Gly Met Glu Asn Met Ser Arg Ala Pro Tyr Leu Ala Asn Asn Ala 
        115                 120                 125             


Arg Trp Gly Tyr Arg Met Gly Asn Ala Lys Phe Val Asp Glu Met Ile 
    130                 135                 140                 


Thr Asp Gly Leu Trp Asp Ala Phe Asn Asp Tyr His Met Gly Ile Thr 
145                 150                 155                 160 


Ala Glu Asn Ile Ala Glu Arg Trp Asn Ile Ser Arg Glu Glu Gln Asp 
                165                 170                 175     


Glu Phe Ala Leu Ala Ser Gln Lys Lys Ala Glu Glu Ala Ile Lys Ser 
            180                 185                 190         


Gly Gln Phe Lys Asp Glu Ile Val Pro Val Val Ile Lys Gly Arg Lys 
        195                 200                 205             


Gly Glu Thr Val Val Asp Thr Asp Glu His Pro Arg Phe Gly Ser Thr 
    210                 215                 220                 


Ile Glu Gly Leu Ala Lys Leu Lys Pro Ala Phe Lys Lys Asp Gly Thr 
225                 230                 235                 240 


Val Thr Ala Gly Asn Ala Ser Gly Leu Asn Asp Cys Ala Ala Val Leu 
                245                 250                 255     


Val Ile Met Ser Ala Glu Lys Ala Lys Glu Leu Gly Val Lys Pro Leu 
            260                 265                 270         


Ala Lys Ile Val Ser Tyr Gly Ser Ala Gly Val Asp Pro Ala Ile Met 
        275                 280                 285             


Gly Tyr Gly Pro Phe Tyr Ala Thr Lys Ala Ala Ile Glu Lys Ala Gly 
    290                 295                 300                 


Trp Thr Val Asp Glu Leu Asp Leu Ile Glu Ser Asn Glu Ala Phe Ala 
305                 310                 315                 320 


Ala Gln Ser Leu Ala Val Ala Lys Asp Leu Lys Phe Asp Met Asn Lys 
                325                 330                 335     


Val Asn Val Asn Gly Gly Ala Ile Ala Leu Gly His Pro Ile Gly Ala 
            340                 345                 350         


Ser Gly Ala Arg Ile Leu Val Thr Leu Val His Ala Met Gln Lys Arg 
        355                 360                 365             


Asp Ala Lys Lys Gly Leu Ala Thr Leu Cys Ile Gly Gly Gly Gln Gly 
    370                 375                 380                 


Thr Ala Ile Leu Leu Glu Lys Cys 
385                 390         


<210>  3
<211>  1179
<212>  DNA
<213>  Clostridium acetobutylicum

<400>  3
atgagagatg tagtaatagt aagtgctgta agaactgcaa taggagcata tggaaaaaca     60

ttaaaggatg tacctgcaac agagttagga gctatagtaa taaaggaagc tgtaagaaga    120

gctaatataa atccaaatga gattaatgaa gttatttttg gaaatgtact tcaagctgga    180

ttaggccaaa acccagcaag acaagcagca gtaaaagcag gattaccttt agaaacacct    240

gcgtttacaa tcaataaggt ttgtggttca ggtttaagat ctataagttt agcagctcaa    300

attataaaag ctggagatgc tgataccatt gtagtaggtg gtatggaaaa tatgtctaga    360

tcaccatatt tgattaacaa tcagagatgg ggtcaaagaa tgggagatag tgaattagtt    420

gatgaaatga taaaggatgg tttgtgggat gcatttaatg gatatcatat gggagtaact    480

gcagaaaata ttgcagaaca atggaatata acaagagaag agcaagatga attttcactt    540

atgtcacaac aaaaagctga aaaagccatt aaaaatggag aatttaagga tgaaatagtt    600

cctgtattaa taaagactaa aaaaggtgaa atagtctttg atcaagatga atttcctaga    660

ttcggaaaca ctattgaagc attaagaaaa cttaaaccta ttttcaagga aaatggtact    720

gttacagcag gtaatgcatc cggattaaat gatggagctg cagcactagt aataatgagc    780

gctgataaag ctaacgctct cggaataaaa ccacttgcta agattacttc ttacggatca    840

tatggggtag atccatcaat aatgggatat ggagcttttt atgcaactaa agctgcctta    900

gataaaatta atttaaaacc tgaagactta gatttaattg aagctaacga ggcatatgct    960

tctcaaagta tagcagtaac tagagattta aatttagata tgagtaaagt taatgttaat   1020

ggtggagcta tagcacttgg acatccaata ggtgcatctg gtgcacgtat tttagtaaca   1080

ttactatacg ctatgcaaaa aagagattca aaaaaaggtc ttgctactct atgtattggt   1140

ggaggtcagg gaacagctct cgtagttgaa agagactaa                          1179


<210>  4
<211>  392
<212>  PRT
<213>  Clostridium acetobutylicum

<400>  4

Met Arg Asp Val Val Ile Val Ser Ala Val Arg Thr Ala Ile Gly Ala 
1               5                   10                  15      


Tyr Gly Lys Thr Leu Lys Asp Val Pro Ala Thr Glu Leu Gly Ala Ile 
            20                  25                  30          


Val Ile Lys Glu Ala Val Arg Arg Ala Asn Ile Asn Pro Asn Glu Ile 
        35                  40                  45              


Asn Glu Val Ile Phe Gly Asn Val Leu Gln Ala Gly Leu Gly Gln Asn 
    50                  55                  60                  


Pro Ala Arg Gln Ala Ala Val Lys Ala Gly Leu Pro Leu Glu Thr Pro 
65                  70                  75                  80  


Ala Phe Thr Ile Asn Lys Val Cys Gly Ser Gly Leu Arg Ser Ile Ser 
                85                  90                  95      


Leu Ala Ala Gln Ile Ile Lys Ala Gly Asp Ala Asp Thr Ile Val Val 
            100                 105                 110         


Gly Gly Met Glu Asn Met Ser Arg Ser Pro Tyr Leu Ile Asn Asn Gln 
        115                 120                 125             


Arg Trp Gly Gln Arg Met Gly Asp Ser Glu Leu Val Asp Glu Met Ile 
    130                 135                 140                 


Lys Asp Gly Leu Trp Asp Ala Phe Asn Gly Tyr His Met Gly Val Thr 
145                 150                 155                 160 


Ala Glu Asn Ile Ala Glu Gln Trp Asn Ile Thr Arg Glu Glu Gln Asp 
                165                 170                 175     


Glu Phe Ser Leu Met Ser Gln Gln Lys Ala Glu Lys Ala Ile Lys Asn 
            180                 185                 190         


Gly Glu Phe Lys Asp Glu Ile Val Pro Val Leu Ile Lys Thr Lys Lys 
        195                 200                 205             


Gly Glu Ile Val Phe Asp Gln Asp Glu Phe Pro Arg Phe Gly Asn Thr 
    210                 215                 220                 


Ile Glu Ala Leu Arg Lys Leu Lys Pro Ile Phe Lys Glu Asn Gly Thr 
225                 230                 235                 240 


Val Thr Ala Gly Asn Ala Ser Gly Leu Asn Asp Gly Ala Ala Ala Leu 
                245                 250                 255     


Val Ile Met Ser Ala Asp Lys Ala Asn Ala Leu Gly Ile Lys Pro Leu 
            260                 265                 270         


Ala Lys Ile Thr Ser Tyr Gly Ser Tyr Gly Val Asp Pro Ser Ile Met 
        275                 280                 285             


Gly Tyr Gly Ala Phe Tyr Ala Thr Lys Ala Ala Leu Asp Lys Ile Asn 
    290                 295                 300                 


Leu Lys Pro Glu Asp Leu Asp Leu Ile Glu Ala Asn Glu Ala Tyr Ala 
305                 310                 315                 320 


Ser Gln Ser Ile Ala Val Thr Arg Asp Leu Asn Leu Asp Met Ser Lys 
                325                 330                 335     


Val Asn Val Asn Gly Gly Ala Ile Ala Leu Gly His Pro Ile Gly Ala 
            340                 345                 350         


Ser Gly Ala Arg Ile Leu Val Thr Leu Leu Tyr Ala Met Gln Lys Arg 
        355                 360                 365             


Asp Ser Lys Lys Gly Leu Ala Thr Leu Cys Ile Gly Gly Gly Gln Gly 
    370                 375                 380                 


Thr Ala Leu Val Val Glu Arg Asp 
385                 390         


<210>  5
<211>  849
<212>  DNA
<213>  Clostridium acetobutylicum

<400>  5
atgaaaaagg tatgtgttat aggtgcaggt actatgggtt caggaattgc tcaggcattt     60

gcagctaaag gatttgaagt agtattaaga gatattaaag atgaatttgt tgatagagga    120

ttagatttta tcaataaaaa tctttctaaa ttagttaaaa aaggaaagat agaagaagct    180

actaaagttg aaatcttaac tagaatttcc ggaacagttg accttaatat ggcagctgat    240

tgcgatttag ttatagaagc agctgttgaa agaatggata ttaaaaagca gatttttgct    300

gacttagaca atatatgcaa gccagaaaca attcttgcat caaatacatc atcactttca    360

ataacagaag tggcatcagc aactaaaaga cctgataagg ttataggtat gcatttcttt    420

aatccagctc ctgttatgaa gcttgtagag gtaataagag gaatagctac atcacaagaa    480

acttttgatg cagttaaaga gacatctata gcaataggaa aagatcctgt agaagtagca    540

gaagcaccag gatttgttgt aaatagaata ttaataccaa tgattaatga agcagttggt    600

atattagcag aaggaatagc ttcagtagaa gacatagata aagctatgaa acttggagct    660

aatcacccaa tgggaccatt agaattaggt gattttatag gtcttgatat atgtcttgct    720

ataatggatg ttttatactc agaaactgga gattctaagt atagaccaca tacattactt    780

aagaagtatg taagagcagg atggcttgga agaaaatcag gaaaaggttt ctacgattat    840

tcaaaataa                                                            849


<210>  6
<211>  282
<212>  PRT
<213>  Clostridium acetobutylicum

<400>  6

Met Lys Lys Val Cys Val Ile Gly Ala Gly Thr Met Gly Ser Gly Ile 
1               5                   10                  15      


Ala Gln Ala Phe Ala Ala Lys Gly Phe Glu Val Val Leu Arg Asp Ile 
            20                  25                  30          


Lys Asp Glu Phe Val Asp Arg Gly Leu Asp Phe Ile Asn Lys Asn Leu 
        35                  40                  45              


Ser Lys Leu Val Lys Lys Gly Lys Ile Glu Glu Ala Thr Lys Val Glu 
    50                  55                  60                  


Ile Leu Thr Arg Ile Ser Gly Thr Val Asp Leu Asn Met Ala Ala Asp 
65                  70                  75                  80  


Cys Asp Leu Val Ile Glu Ala Ala Val Glu Arg Met Asp Ile Lys Lys 
                85                  90                  95      


Gln Ile Phe Ala Asp Leu Asp Asn Ile Cys Lys Pro Glu Thr Ile Leu 
            100                 105                 110         


Ala Ser Asn Thr Ser Ser Leu Ser Ile Thr Glu Val Ala Ser Ala Thr 
        115                 120                 125             


Lys Arg Pro Asp Lys Val Ile Gly Met His Phe Phe Asn Pro Ala Pro 
    130                 135                 140                 


Val Met Lys Leu Val Glu Val Ile Arg Gly Ile Ala Thr Ser Gln Glu 
145                 150                 155                 160 


Thr Phe Asp Ala Val Lys Glu Thr Ser Ile Ala Ile Gly Lys Asp Pro 
                165                 170                 175     


Val Glu Val Ala Glu Ala Pro Gly Phe Val Val Asn Arg Ile Leu Ile 
            180                 185                 190         


Pro Met Ile Asn Glu Ala Val Gly Ile Leu Ala Glu Gly Ile Ala Ser 
        195                 200                 205             


Val Glu Asp Ile Asp Lys Ala Met Lys Leu Gly Ala Asn His Pro Met 
    210                 215                 220                 


Gly Pro Leu Glu Leu Gly Asp Phe Ile Gly Leu Asp Ile Cys Leu Ala 
225                 230                 235                 240 


Ile Met Asp Val Leu Tyr Ser Glu Thr Gly Asp Ser Lys Tyr Arg Pro 
                245                 250                 255     


His Thr Leu Leu Lys Lys Tyr Val Arg Ala Gly Trp Leu Gly Arg Lys 
            260                 265                 270         


Ser Gly Lys Gly Phe Tyr Asp Tyr Ser Lys 
        275                 280         


<210>  7
<211>  786
<212>  DNA
<213>  Clostridium acetobutylicum

<400>  7
atggaactaa acaatgtcat ccttgaaaag gaaggtaaag ttgctgtagt taccattaac     60

agacctaaag cattaaatgc gttaaatagt gatacactaa aagaaatgga ttatgttata    120

ggtgaaattg aaaatgatag cgaagtactt gcagtaattt taactggagc aggagaaaaa    180

tcatttgtag caggagcaga tatttctgag atgaaggaaa tgaataccat tgaaggtaga    240

aaattcggga tacttggaaa taaagtgttt agaagattag aacttcttga aaagcctgta    300

atagcagctg ttaatggttt tgctttagga ggcggatgcg aaatagctat gtcttgtgat    360

ataagaatag cttcaagcaa cgcaagattt ggtcaaccag aagtaggtct cggaataaca    420

cctggttttg gtggtacaca aagactttca agattagttg gaatgggcat ggcaaagcag    480

cttatattta ctgcacaaaa tataaaggca gatgaagcat taagaatcgg acttgtaaat    540

aaggtagtag aacctagtga attaatgaat acagcaaaag aaattgcaaa caaaattgtg    600

agcaatgctc cagtagctgt taagttaagc aaacaggcta ttaatagagg aatgcagtgt    660

gatattgata ctgctttagc atttgaatca gaagcatttg gagaatgctt ttcaacagag    720

gatcaaaagg atgcaatgac agctttcata gagaaaagaa aaattgaagg cttcaaaaat    780

agatag                                                               786


<210>  8
<211>  261
<212>  PRT
<213>  Clostridium acetobutylicum

<400>  8

Met Glu Leu Asn Asn Val Ile Leu Glu Lys Glu Gly Lys Val Ala Val 
1               5                   10                  15      


Val Thr Ile Asn Arg Pro Lys Ala Leu Asn Ala Leu Asn Ser Asp Thr 
            20                  25                  30          


Leu Lys Glu Met Asp Tyr Val Ile Gly Glu Ile Glu Asn Asp Ser Glu 
        35                  40                  45              


Val Leu Ala Val Ile Leu Thr Gly Ala Gly Glu Lys Ser Phe Val Ala 
    50                  55                  60                  


Gly Ala Asp Ile Ser Glu Met Lys Glu Met Asn Thr Ile Glu Gly Arg 
65                  70                  75                  80  


Lys Phe Gly Ile Leu Gly Asn Lys Val Phe Arg Arg Leu Glu Leu Leu 
                85                  90                  95      


Glu Lys Pro Val Ile Ala Ala Val Asn Gly Phe Ala Leu Gly Gly Gly 
            100                 105                 110         


Cys Glu Ile Ala Met Ser Cys Asp Ile Arg Ile Ala Ser Ser Asn Ala 
        115                 120                 125             


Arg Phe Gly Gln Pro Glu Val Gly Leu Gly Ile Thr Pro Gly Phe Gly 
    130                 135                 140                 


Gly Thr Gln Arg Leu Ser Arg Leu Val Gly Met Gly Met Ala Lys Gln 
145                 150                 155                 160 


Leu Ile Phe Thr Ala Gln Asn Ile Lys Ala Asp Glu Ala Leu Arg Ile 
                165                 170                 175     


Gly Leu Val Asn Lys Val Val Glu Pro Ser Glu Leu Met Asn Thr Ala 
            180                 185                 190         


Lys Glu Ile Ala Asn Lys Ile Val Ser Asn Ala Pro Val Ala Val Lys 
        195                 200                 205             


Leu Ser Lys Gln Ala Ile Asn Arg Gly Met Gln Cys Asp Ile Asp Thr 
    210                 215                 220                 


Ala Leu Ala Phe Glu Ser Glu Ala Phe Gly Glu Cys Phe Ser Thr Glu 
225                 230                 235                 240 


Asp Gln Lys Asp Ala Met Thr Ala Phe Ile Glu Lys Arg Lys Ile Glu 
                245                 250                 255     


Gly Phe Lys Asn Arg 
            260     


<210>  9
<211>  1197
<212>  DNA
<213>  Clostridium acetobutylicum

<400>  9
atgatagtaa aagcaaagtt tgtaaaagga tttatcagag atgtacatcc ttatggttgc     60

agaagggaag tactaaatca aatagattat tgtaagaagg ctattgggtt taggggacca    120

aagaaggttt taattgttgg agcctcatct gggtttggtc ttgctactag aatttcagtt    180

gcatttggag gtccagaagc tcacacaatt ggagtatcct atgaaacagg agctacagat    240

agaagaatag gaacagcggg atggtataat aacatatttt ttaaagaatt tgctaaaaaa    300

aaaggattag ttgcaaaaaa cttcattgag gatgcctttt ctaatgaaac caaagataaa    360

gttattaagt atataaagga tgaatttggt aaaatagatt tatttgttta tagtttagct    420

gcgcctagga gaaaggacta taaaactgga aatgtttata cttcaagaat aaaaacaatt    480

ttaggagatt ttgagggacc gactattgat gttgaaagag acgagattac tttaaaaaag    540

gttagtagtg ctagcattga agaaattgaa gaaactagaa aggtaatggg tggagaggat    600

tggcaagagt ggtgtgaaga gctgctttat gaagattgtt tttcggataa agcaactacc    660

atagcatact cgtatatagg atccccaaga acctacaaga tatatagaga aggtactata    720

ggaatagcta aaaaggatct tgaagataag gctaagctta taaatgaaaa acttaacaga    780

gttataggtg gtagagcctt tgtgtctgtg aataaagcat tagttacaaa agcaagtgca    840

tatattccaa cttttcctct ttatgcagct attttatata aggtcatgaa agaaaaaaat    900

attcatgaaa attgtattat gcaaattgag agaatgtttt ctgaaaaaat atattcaaat    960

gaaaaaatac aatttgatga caagggaaga ttaaggatgg acgatttaga gcttagaaaa   1020

gacgttcaag acgaagttga tagaatatgg agtaatatta ctcctgaaaa ttttaaggaa   1080

ttatctgatt ataagggata caaaaaagaa ttcatgaact taaacggttt tgatctagat   1140

ggggttgatt atagtaaaga cctggatata gaattattaa gaaaattaga accttaa      1197


<210>  10
<211>  398
<212>  PRT
<213>  Clostridium acetobutylicum

<400>  10

Met Ile Val Lys Ala Lys Phe Val Lys Gly Phe Ile Arg Asp Val His 
1               5                   10                  15      


Pro Tyr Gly Cys Arg Arg Glu Val Leu Asn Gln Ile Asp Tyr Cys Lys 
            20                  25                  30          


Lys Ala Ile Gly Phe Arg Gly Pro Lys Lys Val Leu Ile Val Gly Ala 
        35                  40                  45              


Ser Ser Gly Phe Gly Leu Ala Thr Arg Ile Ser Val Ala Phe Gly Gly 
    50                  55                  60                  


Pro Glu Ala His Thr Ile Gly Val Ser Tyr Glu Thr Gly Ala Thr Asp 
65                  70                  75                  80  


Arg Arg Ile Gly Thr Ala Gly Trp Tyr Asn Asn Ile Phe Phe Lys Glu 
                85                  90                  95      


Phe Ala Lys Lys Lys Gly Leu Val Ala Lys Asn Phe Ile Glu Asp Ala 
            100                 105                 110         


Phe Ser Asn Glu Thr Lys Asp Lys Val Ile Lys Tyr Ile Lys Asp Glu 
        115                 120                 125             


Phe Gly Lys Ile Asp Leu Phe Val Tyr Ser Leu Ala Ala Pro Arg Arg 
    130                 135                 140                 


Lys Asp Tyr Lys Thr Gly Asn Val Tyr Thr Ser Arg Ile Lys Thr Ile 
145                 150                 155                 160 


Leu Gly Asp Phe Glu Gly Pro Thr Ile Asp Val Glu Arg Asp Glu Ile 
                165                 170                 175     


Thr Leu Lys Lys Val Ser Ser Ala Ser Ile Glu Glu Ile Glu Glu Thr 
            180                 185                 190         


Arg Lys Val Met Gly Gly Glu Asp Trp Gln Glu Trp Cys Glu Glu Leu 
        195                 200                 205             


Leu Tyr Glu Asp Cys Phe Ser Asp Lys Ala Thr Thr Ile Ala Tyr Ser 
    210                 215                 220                 


Tyr Ile Gly Ser Pro Arg Thr Tyr Lys Ile Tyr Arg Glu Gly Thr Ile 
225                 230                 235                 240 


Gly Ile Ala Lys Lys Asp Leu Glu Asp Lys Ala Lys Leu Ile Asn Glu 
                245                 250                 255     


Lys Leu Asn Arg Val Ile Gly Gly Arg Ala Phe Val Ser Val Asn Lys 
            260                 265                 270         


Ala Leu Val Thr Lys Ala Ser Ala Tyr Ile Pro Thr Phe Pro Leu Tyr 
        275                 280                 285             


Ala Ala Ile Leu Tyr Lys Val Met Lys Glu Lys Asn Ile His Glu Asn 
    290                 295                 300                 


Cys Ile Met Gln Ile Glu Arg Met Phe Ser Glu Lys Ile Tyr Ser Asn 
305                 310                 315                 320 


Glu Lys Ile Gln Phe Asp Asp Lys Gly Arg Leu Arg Met Asp Asp Leu 
                325                 330                 335     


Glu Leu Arg Lys Asp Val Gln Asp Glu Val Asp Arg Ile Trp Ser Asn 
            340                 345                 350         


Ile Thr Pro Glu Asn Phe Lys Glu Leu Ser Asp Tyr Lys Gly Tyr Lys 
        355                 360                 365             


Lys Glu Phe Met Asn Leu Asn Gly Phe Asp Leu Asp Gly Val Asp Tyr 
    370                 375                 380                 


Ser Lys Asp Leu Asp Ile Glu Leu Leu Arg Lys Leu Glu Pro 
385                 390                 395             


<210>  11
<211>  1407
<212>  DNA
<213>  Clostridium beijerinckii

<400>  11
atgaataaag acacactaat acctacaact aaagatttaa aagtaaaaac aaatggtgaa     60

aacattaatt taaagaacta caaggataat tcttcatgtt tcggagtatt cgaaaatgtt    120

gaaaatgcta taagcagcgc tgtacacgca caaaagatat tatcccttca ttatacaaaa    180

gagcaaagag aaaaaatcat aactgagata agaaaggccg cattacaaaa taaagaggtc    240

ttggctacaa tgattctaga agaaacacat atgggaagat atgaggataa aatattaaaa    300

catgaattgg tagctaaata tactcctggt acagaagatt taactactac tgcttggtca    360

ggtgataatg gtcttacagt tgtagaaatg tctccatatg gtgttatagg tgcaataact    420

ccttctacga atccaactga aactgtaata tgtaatagca taggcatgat agctgctgga    480

aatgctgtag tatttaacgg acacccatgc gctaaaaaat gtgttgcctt tgctgttgaa    540

atgataaata aggcaattat ttcatgtggc ggtcctgaaa atctagtaac aactataaaa    600

aatccaacta tggagtctct agatgcaatt attaagcatc cttcaataaa acttctttgc    660

ggaactgggg gtccaggaat ggtaaaaacc ctcttaaatt ctggtaagaa agctataggt    720

gctggtgctg gaaatccacc agttattgta gatgatactg ctgatataga aaaggctggt    780

aggagcatca ttgaaggctg ttcttttgat aataatttac cttgtattgc agaaaaagaa    840

gtatttgttt ttgagaatgt tgcagatgat ttaatatcta acatgctaaa aaataatgct    900

gtaattataa atgaagatca agtatcaaaa ttaatagatt tagtattaca aaaaaataat    960

gaaactcaag aatactttat aaacaaaaaa tgggtaggaa aagatgcaaa attattctta   1020

gatgaaatag atgttgagtc tccttcaaat gttaaatgca taatctgcga agtaaatgca   1080

aatcatccat ttgttatgac agaactcatg atgccaatat tgccaattgt aagagttaaa   1140

gatatagatg aagctattaa atatgcaaag atagcagaac aaaatagaaa acatagtgcc   1200

tatatttatt ctaaaaatat agacaaccta aatagatttg aaagagaaat agatactact   1260

atttttgtaa agaatgctaa atcttttgct ggtgttggtt atgaagcaga aggatttaca   1320

actttcacta ttgctggatc tactggtgag ggaataacct ctgcaaggaa ttttacaaga   1380

caaagaagat gtgtacttgc cggctaa                                       1407


<210>  12
<211>  468
<212>  PRT
<213>  Clostridium beijerinckii

<400>  12

Met Asn Lys Asp Thr Leu Ile Pro Thr Thr Lys Asp Leu Lys Val Lys 
1               5                   10                  15      


Thr Asn Gly Glu Asn Ile Asn Leu Lys Asn Tyr Lys Asp Asn Ser Ser 
            20                  25                  30          


Cys Phe Gly Val Phe Glu Asn Val Glu Asn Ala Ile Ser Ser Ala Val 
        35                  40                  45              


His Ala Gln Lys Ile Leu Ser Leu His Tyr Thr Lys Glu Gln Arg Glu 
    50                  55                  60                  


Lys Ile Ile Thr Glu Ile Arg Lys Ala Ala Leu Gln Asn Lys Glu Val 
65                  70                  75                  80  


Leu Ala Thr Met Ile Leu Glu Glu Thr His Met Gly Arg Tyr Glu Asp 
                85                  90                  95      


Lys Ile Leu Lys His Glu Leu Val Ala Lys Tyr Thr Pro Gly Thr Glu 
            100                 105                 110         


Asp Leu Thr Thr Thr Ala Trp Ser Gly Asp Asn Gly Leu Thr Val Val 
        115                 120                 125             


Glu Met Ser Pro Tyr Gly Val Ile Gly Ala Ile Thr Pro Ser Thr Asn 
    130                 135                 140                 


Pro Thr Glu Thr Val Ile Cys Asn Ser Ile Gly Met Ile Ala Ala Gly 
145                 150                 155                 160 


Asn Ala Val Val Phe Asn Gly His Pro Cys Ala Lys Lys Cys Val Ala 
                165                 170                 175     


Phe Ala Val Glu Met Ile Asn Lys Ala Ile Ile Ser Cys Gly Gly Pro 
            180                 185                 190         


Glu Asn Leu Val Thr Thr Ile Lys Asn Pro Thr Met Glu Ser Leu Asp 
        195                 200                 205             


Ala Ile Ile Lys His Pro Ser Ile Lys Leu Leu Cys Gly Thr Gly Gly 
    210                 215                 220                 


Pro Gly Met Val Lys Thr Leu Leu Asn Ser Gly Lys Lys Ala Ile Gly 
225                 230                 235                 240 


Ala Gly Ala Gly Asn Pro Pro Val Ile Val Asp Asp Thr Ala Asp Ile 
                245                 250                 255     


Glu Lys Ala Gly Arg Ser Ile Ile Glu Gly Cys Ser Phe Asp Asn Asn 
            260                 265                 270         


Leu Pro Cys Ile Ala Glu Lys Glu Val Phe Val Phe Glu Asn Val Ala 
        275                 280                 285             


Asp Asp Leu Ile Ser Asn Met Leu Lys Asn Asn Ala Val Ile Ile Asn 
    290                 295                 300                 


Glu Asp Gln Val Ser Lys Leu Ile Asp Leu Val Leu Gln Lys Asn Asn 
305                 310                 315                 320 


Glu Thr Gln Glu Tyr Phe Ile Asn Lys Lys Trp Val Gly Lys Asp Ala 
                325                 330                 335     


Lys Leu Phe Leu Asp Glu Ile Asp Val Glu Ser Pro Ser Asn Val Lys 
            340                 345                 350         


Cys Ile Ile Cys Glu Val Asn Ala Asn His Pro Phe Val Met Thr Glu 
        355                 360                 365             


Leu Met Met Pro Ile Leu Pro Ile Val Arg Val Lys Asp Ile Asp Glu 
    370                 375                 380                 


Ala Ile Lys Tyr Ala Lys Ile Ala Glu Gln Asn Arg Lys His Ser Ala 
385                 390                 395                 400 


Tyr Ile Tyr Ser Lys Asn Ile Asp Asn Leu Asn Arg Phe Glu Arg Glu 
                405                 410                 415     


Ile Asp Thr Thr Ile Phe Val Lys Asn Ala Lys Ser Phe Ala Gly Val 
            420                 425                 430         


Gly Tyr Glu Ala Glu Gly Phe Thr Thr Phe Thr Ile Ala Gly Ser Thr 
        435                 440                 445             


Gly Glu Gly Ile Thr Ser Ala Arg Asn Phe Thr Arg Gln Arg Arg Cys 
    450                 455                 460                 


Val Leu Ala Gly 
465             


<210>  13
<211>  1215
<212>  DNA
<213>  Clostridium acetobutylicum

<400>  13
atggttgatt tcgaatattc aataccaact agaatttttt tcggtaaaga taagataaat     60

gtacttggaa gagagcttaa aaaatatggt tctaaagtgc ttatagttta tggtggagga    120

agtataaaga gaaatggaat atatgataaa gctgtaagta tacttgaaaa aaacagtatt    180

aaattttatg aacttgcagg agtagagcca aatccaagag taactacagt tgaaaaagga    240

gttaaaatat gtagagaaaa tggagttgaa gtagtactag ctataggtgg aggaagtgca    300

atagattgcg caaaggttat agcagcagca tgtgaatatg atggaaatcc atgggatatt    360

gtgttagatg gctcaaaaat aaaaagggtg cttcctatag ctagtatatt aaccattgct    420

gcaacaggat cagaaatgga tacgtgggca gtaataaata atatggatac aaacgaaaaa    480

ctaattgcgg cacatccaga tatggctcct aagttttcta tattagatcc aacgtatacg    540

tataccgtac ctaccaatca aacagcagca ggaacagctg atattatgag tcatatattt    600

gaggtgtatt ttagtaatac aaaaacagca tatttgcagg atagaatggc agaagcgtta    660

ttaagaactt gtattaaata tggaggaata gctcttgaga agccggatga ttatgaggca    720

agagccaatc taatgtgggc ttcaagtctt gcgataaatg gacttttaac atatggtaaa    780

gacactaatt ggagtgtaca cttaatggaa catgaattaa gtgcttatta cgacataaca    840

cacggcgtag ggcttgcaat tttaacacct aattggatgg agtatatttt aaataatgat    900

acagtgtaca agtttgttga atatggtgta aatgtttggg gaatagacaa agaaaaaaat    960

cactatgaca tagcacatca agcaatacaa aaaacaagag attactttgt aaatgtacta   1020

ggtttaccat ctagactgag agatgttgga attgaagaag aaaaattgga cataatggca   1080

aaggaatcag taaagcttac aggaggaacc ataggaaacc taagaccagt aaacgcctcc   1140

gaagtcctac aaatattcaa aaaatctgtg taaaacgcct ccgaagtcct acaaatattc   1200

aaaaaatctg tgtaa                                                    1215


<210>  14
<211>  390
<212>  PRT
<213>  Clostridium acetobutylicum

<400>  14

Met Val Asp Phe Glu Tyr Ser Ile Pro Thr Arg Ile Phe Phe Gly Lys 
1               5                   10                  15      


Asp Lys Ile Asn Val Leu Gly Arg Glu Leu Lys Lys Tyr Gly Ser Lys 
            20                  25                  30          


Val Leu Ile Val Tyr Gly Gly Gly Ser Ile Lys Arg Asn Gly Ile Tyr 
        35                  40                  45              


Asp Lys Ala Val Ser Ile Leu Glu Lys Asn Ser Ile Lys Phe Tyr Glu 
    50                  55                  60                  


Leu Ala Gly Val Glu Pro Asn Pro Arg Val Thr Thr Val Glu Lys Gly 
65                  70                  75                  80  


Val Lys Ile Cys Arg Glu Asn Gly Val Glu Val Val Leu Ala Ile Gly 
                85                  90                  95      


Gly Gly Ser Ala Ile Asp Cys Ala Lys Val Ile Ala Ala Ala Cys Glu 
            100                 105                 110         


Tyr Asp Gly Asn Pro Trp Asp Ile Val Leu Asp Gly Ser Lys Ile Lys 
        115                 120                 125             


Arg Val Leu Pro Ile Ala Ser Ile Leu Thr Ile Ala Ala Thr Gly Ser 
    130                 135                 140                 


Glu Met Asp Thr Trp Ala Val Ile Asn Asn Met Asp Thr Asn Glu Lys 
145                 150                 155                 160 


Leu Ile Ala Ala His Pro Asp Met Ala Pro Lys Phe Ser Ile Leu Asp 
                165                 170                 175     


Pro Thr Tyr Thr Tyr Thr Val Pro Thr Asn Gln Thr Ala Ala Gly Thr 
            180                 185                 190         


Ala Asp Ile Met Ser His Ile Phe Glu Val Tyr Phe Ser Asn Thr Lys 
        195                 200                 205             


Thr Ala Tyr Leu Gln Asp Arg Met Ala Glu Ala Leu Leu Arg Thr Cys 
    210                 215                 220                 


Ile Lys Tyr Gly Gly Ile Ala Leu Glu Lys Pro Asp Asp Tyr Glu Ala 
225                 230                 235                 240 


Arg Ala Asn Leu Met Trp Ala Ser Ser Leu Ala Ile Asn Gly Leu Leu 
                245                 250                 255     


Thr Tyr Gly Lys Asp Thr Asn Trp Ser Val His Leu Met Glu His Glu 
            260                 265                 270         


Leu Ser Ala Tyr Tyr Asp Ile Thr His Gly Val Gly Leu Ala Ile Leu 
        275                 280                 285             


Thr Pro Asn Trp Met Glu Tyr Ile Leu Asn Asn Asp Thr Val Tyr Lys 
    290                 295                 300                 


Phe Val Glu Tyr Gly Val Asn Val Trp Gly Ile Asp Lys Glu Lys Asn 
305                 310                 315                 320 


His Tyr Asp Ile Ala His Gln Ala Ile Gln Lys Thr Arg Asp Tyr Phe 
                325                 330                 335     


Val Asn Val Leu Gly Leu Pro Ser Arg Leu Arg Asp Val Gly Ile Glu 
            340                 345                 350         


Glu Glu Lys Leu Asp Ile Met Ala Lys Glu Ser Val Lys Leu Thr Gly 
        355                 360                 365             


Gly Thr Ile Gly Asn Leu Arg Pro Val Asn Ala Ser Glu Val Leu Gln 
    370                 375                 380                 


Ile Phe Lys Lys Ser Val 
385                 390 


<210>  15
<211>  1170
<212>  DNA
<213>  Clostridium acetobutylicum

<400>  15
atgctaagtt ttgattattc aataccaact aaagtttttt ttggaaaagg aaaaatagac     60

gtaattggag aagaaattaa gaaatatggc tcaagagtgc ttatagttta tggcggagga    120

agtataaaaa ggaacggtat atatgataga gcaacagcta tattaaaaga aaacaatata    180

gctttctatg aactttcagg agtagagcca aatcctagga taacaacagt aaaaaaaggc    240

atagaaatat gtagagaaaa taatgtggat ttagtattag caataggggg aggaagtgca    300

atagactgtt ctaaggtaat tgcagctgga gtttattatg atggcgatac atgggacatg    360

gttaaagatc catctaaaat aactaaagtt cttccaattg caagtatact tactctttca    420

gcaacagggt ctgaaatgga tcaaattgca gtaatttcaa atatggagac taatgaaaag    480

cttggagtag gacatgatga tatgagacct aaattttcag tgttagatcc tacatatact    540

tttacagtac ctaaaaatca aacagcagcg ggaacagctg acattatgag tcacaccttt    600

gaatcttact ttagtggtgt tgaaggtgct tatgtgcagg acggtatagc agaagcaatc    660

ttaagaacat gtataaagta tggaaaaata gcaatggaga agactgatga ttacgaggct    720

agagctaatt tgatgtgggc ttcaagttta gctataaatg gtctattatc acttggtaag    780

gatagaaaat ggagttgtca tcctatggaa cacgagttaa gtgcatatta tgatataaca    840

catggtgtag gacttgcaat tttaacacct aattggatgg aatatattct aaatgacgat    900

acacttcata aatttgtttc ttatggaata aatgtttggg gaatagacaa gaacaaagat    960

aactatgaaa tagcacgaga ggctattaaa aatacgagag aatactttaa ttcattgggt   1020

attccttcaa agcttagaga agttggaata ggaaaagata aactagaact aatggcaaag   1080

caagctgtta gaaattctgg aggaacaata ggaagtttaa gaccaataaa tgcagaggat   1140

gttcttgaga tatttaaaaa atcttattaa                                    1170


<210>  16
<211>  389
<212>  PRT
<213>  Clostridium acetobutylicum

<400>  16

Met Leu Ser Phe Asp Tyr Ser Ile Pro Thr Lys Val Phe Phe Gly Lys 
1               5                   10                  15      


Gly Lys Ile Asp Val Ile Gly Glu Glu Ile Lys Lys Tyr Gly Ser Arg 
            20                  25                  30          


Val Leu Ile Val Tyr Gly Gly Gly Ser Ile Lys Arg Asn Gly Ile Tyr 
        35                  40                  45              


Asp Arg Ala Thr Ala Ile Leu Lys Glu Asn Asn Ile Ala Phe Tyr Glu 
    50                  55                  60                  


Leu Ser Gly Val Glu Pro Asn Pro Arg Ile Thr Thr Val Lys Lys Gly 
65                  70                  75                  80  


Ile Glu Ile Cys Arg Glu Asn Asn Val Asp Leu Val Leu Ala Ile Gly 
                85                  90                  95      


Gly Gly Ser Ala Ile Asp Cys Ser Lys Val Ile Ala Ala Gly Val Tyr 
            100                 105                 110         


Tyr Asp Gly Asp Thr Trp Asp Met Val Lys Asp Pro Ser Lys Ile Thr 
        115                 120                 125             


Lys Val Leu Pro Ile Ala Ser Ile Leu Thr Leu Ser Ala Thr Gly Ser 
    130                 135                 140                 


Glu Met Asp Gln Ile Ala Val Ile Ser Asn Met Glu Thr Asn Glu Lys 
145                 150                 155                 160 


Leu Gly Val Gly His Asp Asp Met Arg Pro Lys Phe Ser Val Leu Asp 
                165                 170                 175     


Pro Thr Tyr Thr Phe Thr Val Pro Lys Asn Gln Thr Ala Ala Gly Thr 
            180                 185                 190         


Ala Asp Ile Met Ser His Thr Phe Glu Ser Tyr Phe Ser Gly Val Glu 
        195                 200                 205             


Gly Ala Tyr Val Gln Asp Gly Ile Ala Glu Ala Ile Leu Arg Thr Cys 
    210                 215                 220                 


Ile Lys Tyr Gly Lys Ile Ala Met Glu Lys Thr Asp Asp Tyr Glu Ala 
225                 230                 235                 240 


Arg Ala Asn Leu Met Trp Ala Ser Ser Leu Ala Ile Asn Gly Leu Leu 
                245                 250                 255     


Ser Leu Gly Lys Asp Arg Lys Trp Ser Cys His Pro Met Glu His Glu 
            260                 265                 270         


Leu Ser Ala Tyr Tyr Asp Ile Thr His Gly Val Gly Leu Ala Ile Leu 
        275                 280                 285             


Thr Pro Asn Trp Met Glu Tyr Ile Leu Asn Asp Asp Thr Leu His Lys 
    290                 295                 300                 


Phe Val Ser Tyr Gly Ile Asn Val Trp Gly Ile Asp Lys Asn Lys Asp 
305                 310                 315                 320 


Asn Tyr Glu Ile Ala Arg Glu Ala Ile Lys Asn Thr Arg Glu Tyr Phe 
                325                 330                 335     


Asn Ser Leu Gly Ile Pro Ser Lys Leu Arg Glu Val Gly Ile Gly Lys 
            340                 345                 350         


Asp Lys Leu Glu Leu Met Ala Lys Gln Ala Val Arg Asn Ser Gly Gly 
        355                 360                 365             


Thr Ile Gly Ser Leu Arg Pro Ile Asn Ala Glu Asp Val Leu Glu Ile 
    370                 375                 380                 


Phe Lys Lys Ser Tyr 
385                 


<210>  17
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  17
caccatggaa ctaaacaatg tcatccttg                                       29


<210>  18
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  18
cctcctatct atttttgaag ccttc                                           25


<210>  19
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  19
caccatgaaa aaggtatgtg ttataggt                                        28


<210>  20
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  20
catttgataa tggggattct tgt                                             23


<210>  21
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  21
caccatgaaa gaagttgtaa tagctagtgc                                      30


<210>  22
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  22
ctagcacttt tctagcaata ttgctg                                          26


<210>  23
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  23
caccatgcta agttttgatt attcaatac                                       29


<210>  24
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  24
ttaataagat tttttaaata tctca                                           25


<210>  25
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  25
caccatggtt gatttcgaat attcaatacc                                      30


<210>  26
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  26
ttacacagat tttttgaata tttgt                                           25


<210>  27
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  27
caccatgaga gatgtagtaa tagtaagtgc tg                                   32


<210>  28
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  28
ccgcaattgt atccatattg aacc                                            24


<210>  29
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  29
caccatgata gtaaaagcaa agtttg                                          26


<210>  30
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  30
gcttaaagct taaaaccgct tctggcg                                         27


<210>  31
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  31
caccatgaat aaagacacac taatacc                                         27


<210>  32
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  32
gccagaccat ctttgaaaat gcgc                                            24


<210>  33
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  33
catgcatgca aaggaggtta gtagaatgaa agaag                                35


<210>  34
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  34
gtcctgcagg gcgcgcccaa tactttctag cacttttc                             38


<210>  35
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  35
catgtcgaca aaggaggtct gtttaatgaa aaaggtatg                            39


<210>  36
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  36
gtcgcatgcc ttgtaaactt attttgaa                                        28


<210>  37
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  37
catagatctg gatccaaagg agggtgagga aatgatagta aaag                      44


<210>  38
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  38
catgtcgacg tgcagccttt ttaaggttct                                      30


<210>  39
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  39
catgaattca cgcgtaaagg aggtattagt catggaac                             38


<210>  40
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  40
gtcggatccc ttacctccta tctatttttg                                      30


<210>  41
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  41
catgcccggg ggtcaccaaa ggaggaatag ttcatgaata aa                        42


<210>  42
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  42
catggttaac aagaagttag ccggcaagta ca                                   32


<210>  43
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  43
catggttaac aaaggagggg ttaaaatggt tgatttcgaa t                         41


<210>  44
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  44
catggcatgc gtttaaacgt aggtttacac agatttt                              37


<210>  45
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  45
gtaaaacgac ggccagt                                                    17


<210>  46
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  46
aacagctatg accatg                                                     16


<210>  47
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  47
gcaggagatg ctgacgtaat aa                                              22


<210>  48
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  48
ccaacctgct ttttcaatag ctgc                                            24


<210>  49
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  49
cagagatggg gtcaaagaat g                                               21


<210>  50
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  50
gtggttttat tccgagagcg                                                 20


<210>  51
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  51
ggtctatact tagaatctcc                                                 20


<210>  52
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  52
cggaacagtt gaccttaata tggc                                            24


<210>  53
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  53
gcctcatctg ggtttggtct tg                                              22


<210>  54
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  54
cgcctaggag aaaggactat aaaactgg                                        28


<210>  55
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  55
cagagttata ggtggtagag cc                                              22


<210>  56
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  56
ccatcccgct gttcctattc ttct                                            24


<210>  57
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  57
ccaatcctct ccacccatta cc                                              22


<210>  58
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  58
cgtccatcct taatcttccc                                                 20


<210>  59
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  59
ccaactatgg aatccctaga tgc                                             23


<210>  60
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  60
gcatagtctg cgaagtaaat gc                                              22


<210>  61
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  61
ggatctactg gtgaaggcat aacc                                            24


<210>  62
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  62
ggcatcatga gttctgtcat gac                                             23


<210>  63
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  63
gccttcaatg atactcttac cagcc                                           25


<210>  64
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  64
gcatttccag cagctatcat gc                                              22


<210>  65
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  65
ccttcccata tgtgtttctt cc                                              22


<210>  66
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  66
gttgaagtag tactagctat ag                                              22


<210>  67
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  67
gacataacac acggcgtagg gc                                              22


<210>  68
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  68
taagtgtaca ctccaattag tg                                              22


<210>  69
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  69
gccatctaac acaatatccc atgg                                            24


<210>  70
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  70
gcgatacatg ggacatggtt aaag                                            24


<210>  71
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  71
tgcacttaac tcgtgttcca ta                                              22


<210>  72
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  72
gttagccggc aagtacacat c                                               21


<210>  73
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  73
actttctttc gcctgtttca c                                               21


<210>  74
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  74
catgaagctt ggcgcgccgg gacgcgtttt tgaaaataat gaaaact                   47


<210>  75
<211>  79
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  75
catgaagctt gtttaaactc ggtgaccttg aaaataatga aaacttatat tgttttgaaa     60

ataatgaaaa cttatattg                                                  79


<210>  76
<211>  1197
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized CAC0462 gene from Clostridium acetobutylicum

<400>  76
atgattgtga aagcaaaatt cgtgaaagga ttcattcgcg atgtgcaccc ttatgggtgc     60

cgccgtgaag ttctgaatca gatcgactac tgcaaaaaag ccattggctt tcgcggccca    120

aagaaagtgc tgatcgttgg tgcttcctct ggcttcggtc tggctacccg catttccgtg    180

gcgttcggtg gcccagaagc ccacactatc ggcgtcagct atgaaaccgg tgcgaccgat    240

cgccgtattg gcacagcagg gtggtataac aatattttct ttaaagaatt tgccaaaaag    300

aaaggcctgg tggcaaaaaa ctttatcgaa gacgccttct cgaacgaaac caaggacaaa    360

gtcatcaaat atattaaaga cgaatttggc aaaatcgatc tgttcgttta ctcgctggca    420

gcaccgcgtc gtaaggatta taagactggg aacgtttata cctcacgtat taaaacgatc    480

ctgggtgatt ttgaagggcc gactatcgat gtggaacgtg atgaaattac actgaaaaag    540

gtctcatctg cgtcaatcga agagattgaa gaaacccgta aggtgatggg cggcgaagat    600

tggcaagagt ggtgtgaaga actgctgtac gaagattgtt tcagtgataa agccaccacc    660

atcgcctatt cctatatcgg ttctcctcgc acctacaaaa tctaccgcga aggcactatc    720

ggcattgcga aaaaggatct ggaagataag gcaaaactga tcaacgagaa gctgaatcgc    780

gtcattggcg ggcgcgcatt cgttagcgtg aataaagccc tggttactaa ggcgagcgca    840

tatattccga cctttcctct gtacgccgca attctgtata aagttatgaa agaaaagaat    900

attcacgaaa actgcattat gcaaattgaa cgcatgtttt ccgagaaaat ttattcaaat    960

gaaaagattc aatttgatga taaaggtcgt ctgcgtatgg atgacctgga gctgcgtaag   1020

gatgttcagg atgaagtaga ccgtatttgg agcaatatta caccggagaa ttttaaggaa   1080

ctgagcgact ataaaggcta caaaaaagaa tttatgaacc tgaatggatt tgatctggac   1140

ggcgtggatt attcaaagga tctggacatt gaactgctgc gcaaactgga accataa      1197


<210>  77
<211>  1224
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Condon optimized EgTER

<400>  77
atggcgatgt ttacgaccac cgcaaaagtt attcagccga aaattcgtgg ttttatttgc     60

accaccaccc acccgattgg ttgcgaaaaa cgtgttcagg aagaaatcgc atacgcacgc    120

gcgcacccgc cgaccagccc gggtccgaaa cgtgtgctgg ttattggctg cagtacgggc    180

tatggcctga gcacccgtat caccgcggcc tttggttatc aggccgcaac cctgggcgtg    240

tttctggcag gcccgccgac caaaggccgt ccggccgcgg cgggttggta taatacggtt    300

gcgttcgaaa aagccgccct ggaagcaggt ctgtatgcac gttctctgaa tggtgatgcg    360

ttcgattcta ccacgaaagc ccgcaccgtg gaagcaatta aacgtgatct gggtaccgtt    420

gatctggtgg tgtatagcat tgcagcgccg aaacgtaccg atccggccac cggcgtgctg    480

cataaagcgt gcctgaaacc gattggtgca acctacacca atcgtacggt gaacaccgat    540

aaagcagaag ttaccgatgt gagtattgaa ccggccagtc cggaagaaat cgcagatacc    600

gtgaaagtta tgggtggcga agattgggaa ctgtggattc aggcactgag cgaagccggc    660

gtgctggccg aaggcgcaaa aaccgttgcg tattcttata ttggcccgga aatgacgtgg    720

ccggtgtatt ggagtggcac cattggcgaa gccaaaaaag atgttgaaaa agcggcgaaa    780

cgcatcaccc agcagtacgg ctgtccggcg tatccggttg ttgccaaagc gctggtgacc    840

caggccagta gcgccattcc ggtggtgccg ctgtatattt gcctgctgta tcgtgttatg    900

aaagaaaaag gcacccatga aggctgcatt gaacagatgg tgcgtctgct gacgacgaaa    960

ctgtatccgg aaaatggtgc gccgatcgtg gatgaagcgg gccgtgtgcg tgttgatgat   1020

tgggaaatgg cagaagatgt tcagcaggca gttaaagatc tgtggagcca ggtgagtacg   1080

gccaatctga aagatattag cgattttgca ggttatcaga ccgaatttct gcgtctgttt   1140

ggctttggta ttgatggtgt ggattacgat cagccggttg atgttgaagc ggatctgccg   1200

agcgccgccc agcagtaagt cgac                                          1224


<210>  78
<211>  1498
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized ald gene

<400>  78
cggtacctcg cgaatgcatc tagatccaat catgcccggg ggtcaccaaa ggaggaatag     60

ttcatgaata aagatacgct gattccgacc acgaaagatc tgaaagtgaa aaccaacggt    120

gaaaatatca acctgaaaaa ttataaagat aatagcagct gcttcggcgt gtttgaaaat    180

gttgaaaacg ccatttcttc tgccgttcac gcacagaaaa tcctgtctct gcactatacc    240

aaagaacagc gcgaaaaaat cattaccgaa attcgcaaag cggccctgca gaataaagaa    300

gttctggcga ccatgatcct ggaagaaacc catatgggtc gttacgaaga taaaatcctg    360

aaacatgaac tggtggcgaa atacacgccg ggtacggaag atctgaccac gaccgcatgg    420

agcggtgata acggtctgac cgtggtggaa atgtctccgt atggcgttat tggcgcaatt    480

acgccgagca cgaatccgac cgaaacggtt atttgtaaca gtattggcat gattgcagcc    540

ggtaatgcag tggttttcaa tggtcatccg tgcgccaaaa aatgtgtggc gtttgccgtt    600

gaaatgatta acaaagcgat tattagctgc ggtggcccgg aaaatctggt gacgacgatt    660

aaaaacccga ccatggaaag tctggatgcg attattaaac atccgtctat taaactgctg    720

tgtggtaccg gcggtccggg tatggtgaaa accctgctga attctggcaa aaaagcaatt    780

ggcgcgggtg cgggtaatcc gccggttatc gttgatgata ccgcggatat tgaaaaagca    840

ggccgcagta ttattgaagg ttgtagcttt gataataacc tgccgtgcat tgccgaaaaa    900

gaagtgtttg ttttcgaaaa tgtggcggat gatctgatca gcaacatgct gaaaaataac    960

gccgtgatta ttaacgaaga tcaggtgtct aaactgattg atctggttct gcagaaaaac   1020

aacgaaacgc aggaatattt cattaataaa aaatgggttg gtaaagatgc gaaactgttc   1080

ctggatgaaa tcgatgtgga aagtccgagc aacgtgaaat gtatcatctg cgaagtgaat   1140

gccaaccatc cgtttgttat gacggaactg atgatgccga ttctgccgat tgttcgtgtt   1200

aaagatattg atgaagccat caaatatgcg aaaattgcgg aacagaaccg caaacacagc   1260

gcatatattt acagtaaaaa catcgataac ctgaaccgtt tcgaacgtga aattgatacc   1320

accatctttg tgaaaaatgc caaaagtttt gcgggtgttg gctatgaagc cgaaggcttt   1380

accacgttca ccattgcagg ttctaccggc gaaggtatta ccagcgcgcg taattttacc   1440

cgccagcgcc gctgtgtgct ggcgggttaa gttaacccaa tatcggatcc cgggcccg     1498


<210>  79
<211>  6509
<212>  DNA
<213>  Artificial sequence

<220>
<223>  plasmid pFP988

<400>  79
tcgaggcccc gcacatacga aaagactggc tgaaaacatt gagcctttga tgactgatga     60

tttggctgaa gaagtggatc gattgtttga gaaaagaaga agaccataaa aataccttgt    120

ctgtcatcag acagggtatt ttttatgctg tccagactgt ccgctgtgta aaaaatagga    180

ataaaggggg gttgttatta ttttactgat atgtaaaata taatttgtat aaggaattgt    240

gagcggataa caattcctac gaaaatgaga gggagaggaa acatgattca aaaacgaaag    300

cggacagttt cgttcagact tgtgcttatg tgcacgctgt tatttgtcag tttgccgatt    360

acaaaaacat cagccggatc ccaccatcac catcaccatt aagaattcct agaaactcca    420

agctatcttt aaaaaatcta gtaaatgcac gagcaacatc ttttgttgct cagtgcattt    480

tttattttgt acactagata tttcttctcc gcttaaatca tcaaagaaat ctttatcact    540

tgtaaccagt ccgtccacat gtcgaattgc atctgaccga attttacgtt tccctgaata    600

attctcatca atcgtttcat caattttatc tttatacttt atattttgtg cgttaatcaa    660

atcataattt ttatatgttt cctcatgatt tatgtcttta ttattatagt ttttattctc    720

tctttgatta tgtctttgta tcccgtttgt attacttgat cctttaactc tggcaaccct    780

caaaattgaa tgagacatgc tacacctccg gataataaat atatataaac gtatatagat    840

ttcataaagt ctaacacact agacttattt acttcgtaat taagtcgtta aaccgtgtgc    900

tctacgacca aaactataaa acctttaaga actttctttt tttacaagaa aaaagaaatt    960

agataaatct ctcatatctt ttattcaata atcgcatccg attgcagtat aaatttaacg   1020

atcactcatc atgttcatat ttatcagagc tcgtgctata attatactaa ttttataagg   1080

aggaaaaaat atgggcattt ttagtatttt tgtaatcagc acagttcatt atcaaccaaa   1140

caaaaaataa gtggttataa tgaatcgtta ataagcaaaa ttcatataac caaattaaag   1200

agggttataa tgaacgagaa aaatataaaa cacagtcaaa actttattac ttcaaaacat   1260

aatatagata aaataatgac aaatataaga ttaaatgaac atgataatat ctttgaaatc   1320

ggctcaggaa aaggccattt tacccttgaa ttagtaaaga ggtgtaattt cgtaactgcc   1380

attgaaatag accataaatt atgcaaaact acagaaaata aacttgttga tcacgataat   1440

ttccaagttt taaacaagga tatattgcag tttaaatttc ctaaaaacca atcctataaa   1500

atatatggta atatacctta taacataagt acggatataa tacgcaaaat tgtttttgat   1560

agtatagcta atgagattta tttaatcgtg gaatacgggt ttgctaaaag attattaaat   1620

acaaaacgct cattggcatt acttttaatg gcagaagttg atatttctat attaagtatg   1680

gttccaagag aatattttca tcctaaacct aaagtgaata gctcacttat cagattaagt   1740

agaaaaaaat caagaatatc acacaaagat aaacaaaagt ataattattt cgttatgaaa   1800

tgggttaaca aagaatacaa gaaaatattt acaaaaaatc aatttaacaa ttccttaaaa   1860

catgcaggaa ttgacgattt aaacaatatt agctttgaac aattcttatc tcttttcaat   1920

agctataaat tatttaataa gtaagttaag ggatgcagtt catcgatgaa ggcaactaca   1980

gctcaggcga caaccatacg ctgagagatc ctcactacgt agaagataaa ggccacaaat   2040

acttagtatt tgaagcaaac actggaactg aagatggcta ccaaggcgaa gaatctttat   2100

ttaacaaagc atactatggc aaaagcacat cattcttccg tcaagaaagt caaaaacttc   2160

tgcaaagcga taaaaaacgc acggctgagt tagcaaacgg cgctctcggt atgattgagc   2220

taaacgatga ttacacactg aaaaaagtga tgaaaccgct gattgcatct aacacagtaa   2280

cagatgaaat tgaacgcgcg aacgtcttta aaatgaacgg caaatggtac ctgttcactg   2340

actcccgcgg atcaaaaatg acgattgacg gcattacgtc taacgatatt tacatgcttg   2400

gttatgtttc taattcttta actggcccat acaagccgct gaacaaaact ggccttgtgt   2460

taaaaatgga tcttgatcct aacgatgtaa cctttactta ctcacacttc gctgtacctc   2520

aagcgaaagg aaacaatgtc gtgattacaa gctatatgac aaacagagga ttctacgcag   2580

acaaacaatc aacgtttgcg ccaagcttgc atgcgagagt agggaactgc caggcatcaa   2640

ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg   2700

aacgctctcc tgagtaggac aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg   2760

cccggagggt ggcgggcagg acgcccgcca taaactgcca ggcatcaaat taagcagaag   2820

gccatcctga cggatggcct ttttgcgttt ctacaaactc tttttgttta tttttctaaa   2880

tacattcaaa tatgtatccg ctcatgctcc ggatctgcat cgcaggatgc tgctggctac   2940

cctgtggaac acctacatct gtattaacga agcgctggca ttgaccctga gtgatttttc   3000

tctggtcccg ccgcatccat accgccagtt gtttaccctc acaacgttcc agtaaccggg   3060

catgttcatc atcagtaacc cgtatcgtga gcatcctctc tcgtttcatc ggtatcatta   3120

cccccatgaa cagaaattcc cccttacacg gaggcatcaa gtgaccaaac aggaaaaaac   3180

cgcccttaac atggcccgct ttatcagaag ccagacatta acgcttctgg agaaactcaa   3240

cgagctggac gcggatgaac aggcagacat ctgtgaatcg cttcacgacc acgctgatga   3300

gctttaccgc agctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca   3360

gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca   3420

gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga   3480

tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac   3540

catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct   3600

tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca   3660

gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac   3720

atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt   3780

ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg   3840

cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc   3900

tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc   3960

gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc   4020

aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac   4080

tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt   4140

aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct   4200

aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc   4260

ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt   4320

ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg   4380

atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc   4440

atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa   4500

tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag   4560

gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg   4620

tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga   4680

gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag   4740

cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa   4800

gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc   4860

atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca   4920

aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg   4980

atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat   5040

aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc   5100

aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg   5160

gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg   5220

gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt   5280

gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca   5340

ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata   5400

ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac   5460

atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa   5520

gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt   5580

atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg   5640

cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt   5700

cagggcgcgt cagcgggtgt tcatgtgcgt aactaacttg ccatcttcaa acaggagggc   5760

tggaagaagc agaccgctaa cacagtacat aaaaaaggag acatgaacga tgaacatcaa   5820

aaagtttgca aaacaagcaa cagtattaac ctttactacc gcactgctgg caggaggcgc   5880

aactcaagcg tttgcgaaag aaacgaacca aaagccatat aaggaaacat acggcatttc   5940

ccatattaca cgccatgata tgctgcaaat ccctgaacag caaaaaaatg aaaaatatca   6000

agttcctgaa ttcgattcgt ccacaattaa aaatatctct tctgcaaaag gcctggacgt   6060

ttgggacagc tggccattac aaaacgctga cggcactgtc gcaaactatc acggctacca   6120

catcgtcttt gcattagccg gagatcctaa aaatgcggat gacacatcga tttacatgtt   6180

ctatcaaaaa gtcggcgaaa cttctattga cagctggaaa aacgctggcc gcgtctttaa   6240

agacagcgac aaattcgatg caaatgattc tatcctaaaa gaccaaacac aagaatggtc   6300

aggttcagcc acatttacat ctgacggaaa aatccgttta ttctacactg atttctccgg   6360

taaacattac ggcaaacaaa cactgacaac tgcacaagtt aacgtatcag catcagacag   6420

ctctttgaac atcaacggtg tagaggatta taaatcaatc tttgacggtg acggaaaaac   6480

gtatcaaaat gtacagcatg ccacgcgtc                                     6509


<210>  80
<211>  46
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer N85

<400>  80
catagatctg gatccaaagg agggtgagga aatggcgatg tttacg                    46


<210>  81
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer N86

<400>  81
gtcgacttac tgctgggcgg                                                 20


<210>  82
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  T7 primer

<400>  82
taatacgact cactataggg                                                 20


<210>  83
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer Trc99af

<400>  83
ttgacaatta atcatccggc                                                 20


<210>  84
<211>  21
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer N5SeqF4

<400>  84
ggtcaactgt tccggaaatt c                                               21


<210>  85
<211>  46
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer T-ald(BamHI)

<400>  85
tgatctggat ccaagaagga gcccttcacc atgaataaag acacac                    46


<210>  86
<211>  54
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer B-ald(ETGER)

<400>  86
catcgccatt tcctcaccct cctttttagc cggcaagtac acatcttctt tgtc           54


<210>  87
<211>  42
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer T-Ptrc(BspEI)

<400>  87
ttccgtactt ccggacgact gcacggtgca ccaatgcttc tg                        42


<210>  88
<211>  43
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer B-aldopt(BspEI)

<400>  88
cggatcttaa gtactttaac ccgccagcac acagcggcgc tgg                       43


<210>  89
<211>  32
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer T-BspEIAatII

<400>  89
ccggatcatg ataataatgg tttcttagac gt                                   32


<210>  90
<211>  24
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer B-BspEIAatII

<400>  90
ctaagaaacc attattatca tgat                                            24


<210>  91
<211>  42
<212>  DNA
<213>  artificial sequence

<220>
<223>  1.6GI promoter

<400>  91
gcccttgaca atgccacatc ctgagcaaat aattcaacca ct                        42


<210>  92
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  1.5GI promoter

<400>  92
gcccttgact atgccacatc ctgagcaaat aattcaacca ct                        42


<210>  93
<211>  39
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer AFBamHI

<400>  93
cattggatcc atgaataaag acacactaat acctacaac                            39


<210>  94
<211>  39
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer ARAat2

<400>  94
catgacgtca ctagtgttaa caagaagtta gccggcaag                            39


<210>  95
<211>  50
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer Forward 1(E)

<400>  95
catgttaaca aaggaggaaa gatctatggc gatgtttacg accaccgcaa                50


<210>  96
<211>  43
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer bottom reverse 1(E)

<400>  96
cccctccttt ggcgcgcctt actgctgggc ggcgctcggc aga                       43


<210>  97
<211>  51
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer Top Forward 2(B)

<400>  97
gcccagcagt aaggcgcgcc aaaggagggg ttaaaatggt tgatttcgaa t              51


<210>  98
<211>  42
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer reverse 2(B)

<400>  98
gtcgacgtca tactagttta cacagatttt ttgaatattt gt                        42


<210>  99
<211>  47
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer Pamy/lacOF

<400>  99
cattgtacag aattcgagct ctcgaggccc cgcacatacg aaaagac                   47


<210>  100
<211>  52
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer Pam/la cOR

<400>  100
cattgtacag tttaaacata ggtcaccctc attttcgtag gaattgttat cc             52


<210>  101
<211>  52
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer Spac F

<400>  101
cattgtacag tttaaacata ggtcaccctc attttcgtag gaattgttat cc             52


<210>  102
<211>  36
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer Spac R

<400>  102
catgtttaaa cggtgaccca agctggggat ccgcgg                               36


<210>  103
<211>  44
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer Top TF

<400>  103
cattggtcac cattcccggg catgcaaagg aggttagtag aatg                      44


<210>  104
<211>  51
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer Bot TR

<400>  104
cctttacgcg accggtacta gtcaagtcga cagggcgcgc ccaatacttt c              51


<210>  105
<211>  57
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer Top CF

<400>  105
cgcgccctgt cgacttgact agtaccggtc gcgtaaagga ggtattagtc atggaac        57


<210>  106
<211>  38
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer Bot CR

<400>  106
catcgtttaa acttggatcc agatccctta cctcctat                             38


<210>  107
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer N3SeqF1

<400>  107
ccatcatacc atactgaccc                                                 20


<210>  108
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer N3SeqF2

<400>  108
gctactggag cattgctcac                                                 20


<210>  109
<211>  24
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer N3SeqF3

<400>  109
ccattaacag ctgctattac aggc                                            24


<210>  110
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer N4SeqR3

<400>  110
ggtctcggaa taacacctgg                                                 20


<210>  111
<211>  22
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer N5SeqF3

<400>  111
caagcttcat aacaggagct gg                                              22


<210>  112
<211>  22
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer N7SeqR2

<400>  112
atcccacaat ccgtcagtga tc                                              22


<210>  113
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer N31SeqF1

<400>  113
ctgagataag aaaggccgca                                                 20


<210>  114
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer N62SeqF2

<400>  114
caaccctggg cgtgtttctg                                                 20


<210>  115
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer N62SeqF3

<400>  115
gtggcgaaga ttgggaactg                                                 20


<210>  116
<211>  24
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer N62SeqF4

<400>  116
gggaaatggc agaagatgtt cagc                                            24


<210>  117
<211>  24
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer N63SeqR1

<400>  117
cggtctgata acctgcaaaa tcgc                                            24


<210>  118
<211>  21
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer N63SeqR2

<400>  118
caccagcgct ttggcaacaa c                                               21


<210>  119
<211>  23
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer N63SeqR3

<400>  119
gaacgtgcat acagacctgc ttc                                             23


<210>  120
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer N63SeqR4

<400>  120
cggctgaata acttttgcgg                                                 20


<210>  121
<211>  24
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer Pamy SeqF2

<400>  121
gcctttgatg actgatgatt tggc                                            24


<210>  122
<211>  24
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer Pamy SeqF

<400>  122
tctccggtaa acattacggc aaac                                            24


<210>  123
<211>  24
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer Pamy seqR

<400>  123
cggtcagatg caattcgaca tgtg                                            24


<210>  124
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer SpacF Seq

<400>  124
gaagtggtca agacctcact                                                 20


<210>  125
<211>  24
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer sacB Up

<400>  125
cgggtttgtt actgataaag cagg                                            24


<210>  126
<211>  24
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer sacB Dn

<400>  126
cggttagcca tttgcctgct ttta                                            24


<210>  127
<211>  22
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer HT R

<400>  127
acaaagatct ccatggacgc gt                                              22


<210>  128
<211>  1185
<212>  DNA
<213>  Escherichia coli

<400>  128
atgaaaaatt gtgtcatcgt cagtgcggta cgtactgcta tcggtagttt taacggttca     60

ctcgcttcca ccagcgccat cgacctgggg gcgacagtaa ttaaagccgc cattgaacgt    120

gcaaaaatcg attcacaaca cgttgatgaa gtgattatgg gtaacgtgtt acaagccggg    180

ctggggcaaa atccggcgcg tcaggcactg ttaaaaagcg ggctggcaga aacggtgtgc    240

ggattcacgg tcaataaagt atgtggttcg ggtcttaaaa gtgtggcgct tgccgcccag    300

gccattcagg caggtcaggc gcagagcatt gtggcggggg gtatggaaaa tatgagttta    360

gccccctact tactcgatgc aaaagcacgc tctggttatc gtcttggaga cggacaggtt    420

tatgacgtaa tcctgcgcga tggcctgatg tgcgccaccc atggttatca tatggggatt    480

accgccgaaa acgtggctaa agagtacgga attacccgtg aaatgcagga tgaactggcg    540

ctacattcac agcgtaaagc ggcagccgca attgagtccg gtgcttttac agccgaaatc    600

gtcccggtaa atgttgtcac tcgaaagaaa accttcgtct tcagtcaaga cgaattcccg    660

aaagcgaatt caacggctga agcgttaggt gcattgcgcc cggccttcga taaagcagga    720

acagtcaccg ctgggaacgc gtctggtatt aacgacggtg ctgccgctct ggtgattatg    780

gaagaatctg cggcgctggc agcaggcctt acccccctgg ctcgcattaa aagttatgcc    840

agcggtggcg tgccccccgc attgatgggt atggggccag tacctgccac gcaaaaagcg    900

ttacaactgg cggggctgca actggcggat attgatctca ttgaggctaa tgaagcattt    960

gctgcacagt tccttgccgt tgggaaaaac ctgggctttg attctgagaa agtgaatgtc   1020

aacggcgggg ccatcgcgct cgggcatcct atcggtgcca gtggtgctcg tattctggtc   1080

acactattac atgccatgca ggcacgcgat aaaacgctgg ggctggcaac actgtgcatt   1140

ggcggcggtc agggaattgc gatggtgatt gaacggttga attaa                   1185


<210>  129
<211>  394
<212>  PRT
<213>  Escherichia coli

<400>  129

Met Lys Asn Cys Val Ile Val Ser Ala Val Arg Thr Ala Ile Gly Ser 
1               5                   10                  15      


Phe Asn Gly Ser Leu Ala Ser Thr Ser Ala Ile Asp Leu Gly Ala Thr 
            20                  25                  30          


Val Ile Lys Ala Ala Ile Glu Arg Ala Lys Ile Asp Ser Gln His Val 
        35                  40                  45              


Asp Glu Val Ile Met Gly Asn Val Leu Gln Ala Gly Leu Gly Gln Asn 
    50                  55                  60                  


Pro Ala Arg Gln Ala Leu Leu Lys Ser Gly Leu Ala Glu Thr Val Cys 
65                  70                  75                  80  


Gly Phe Thr Val Asn Lys Val Cys Gly Ser Gly Leu Lys Ser Val Ala 
                85                  90                  95      


Leu Ala Ala Gln Ala Ile Gln Ala Gly Gln Ala Gln Ser Ile Val Ala 
            100                 105                 110         


Gly Gly Met Glu Asn Met Ser Leu Ala Pro Tyr Leu Leu Asp Ala Lys 
        115                 120                 125             


Ala Arg Ser Gly Tyr Arg Leu Gly Asp Gly Gln Val Tyr Asp Val Ile 
    130                 135                 140                 


Leu Arg Asp Gly Leu Met Cys Ala Thr His Gly Tyr His Met Gly Ile 
145                 150                 155                 160 


Thr Ala Glu Asn Val Ala Lys Glu Tyr Gly Ile Thr Arg Glu Met Gln 
                165                 170                 175     


Asp Glu Leu Ala Leu His Ser Gln Arg Lys Ala Ala Ala Ala Ile Glu 
            180                 185                 190         


Ser Gly Ala Phe Thr Ala Glu Ile Val Pro Val Asn Val Val Thr Arg 
        195                 200                 205             


Lys Lys Thr Phe Val Phe Ser Gln Asp Glu Phe Pro Lys Ala Asn Ser 
    210                 215                 220                 


Thr Ala Glu Ala Leu Gly Ala Leu Arg Pro Ala Phe Asp Lys Ala Gly 
225                 230                 235                 240 


Thr Val Thr Ala Gly Asn Ala Ser Gly Ile Asn Asp Gly Ala Ala Ala 
                245                 250                 255     


Leu Val Ile Met Glu Glu Ser Ala Ala Leu Ala Ala Gly Leu Thr Pro 
            260                 265                 270         


Leu Ala Arg Ile Lys Ser Tyr Ala Ser Gly Gly Val Pro Pro Ala Leu 
        275                 280                 285             


Met Gly Met Gly Pro Val Pro Ala Thr Gln Lys Ala Leu Gln Leu Ala 
    290                 295                 300                 


Gly Leu Gln Leu Ala Asp Ile Asp Leu Ile Glu Ala Asn Glu Ala Phe 
305                 310                 315                 320 


Ala Ala Gln Phe Leu Ala Val Gly Lys Asn Leu Gly Phe Asp Ser Glu 
                325                 330                 335     


Lys Val Asn Val Asn Gly Gly Ala Ile Ala Leu Gly His Pro Ile Gly 
            340                 345                 350         


Ala Ser Gly Ala Arg Ile Leu Val Thr Leu Leu His Ala Met Gln Ala 
        355                 360                 365             


Arg Asp Lys Thr Leu Gly Leu Ala Thr Leu Cys Ile Gly Gly Gly Gln 
    370                 375                 380                 


Gly Ile Ala Met Val Ile Glu Arg Leu Asn 
385                 390                 


<210>  130
<211>  1182
<212>  DNA
<213>  Bacillus subtilis

<400>  130
ttaatgaacc tgcactaaga cggcgtctcc ctgtgctgcc ccgctgcaaa tagcggcaac     60

gcccagaccc cctccccgtc gctttaattc ataaacaagc gtcatgagaa ttctcgcacc    120

gctcgcgccg atcgggtggc cgagcgcgat cgcaccgcca ttcacattta ctttttcaag    180

atcgaaacct acgatttttt cacatgtcaa aacaactgaa gcaaaagctt catttacttc    240

aaacaagtca atatcttgga cagttaaacc attctttttc aggagcttgt taatagcaaa    300

ccctggcgct gccgccagct cgtgcgctgg cattcccgta gttgaaaaac caagaattgt    360

agccagaggc cgtttgccaa gctcagcagc tttttcctca gacatcagca cgaacgcgcc    420

ggctccgtca ttgactccag gagcattgcc ggctgtgata gaaccgtcac ttgcataaat    480

cggagcaagt tttgcgagct gatccagact tgtgtcacgg cgaatcgctt catctttatc    540

aacaacgttt ggttttcctt ttcgaccgat ccagttgacg ggaacaattt catcctgaaa    600

cttcccttca tcggcggcct tagctgccct tgcatgactt ctcaacgccc attcgtcctg    660

ctctcttcgt gagattgcat attccttggc agctgtattt ccgtgaacag ccatgtgcac    720

ctcgtcaaat gcgcacgtta atccgtcata caccattaag tccctaagct cgccgtcccc    780

catccgtgct ccccagcgcc cggcgggaac ggcatacgga atattgctca tgctttccat    840

cccccccgca acaagtatgt ccgcatcctg cgcccgaatc atttgatcac ataaagtgac    900

agcgcgaagg ccggaagcac agactttatt cagtgtttct gacggcacac tccaaggcat    960

tcccgccaga cgggcagctt gacgggaagg tatctgccct gagccggcct ggacaaccat   1020

gcccatgacg tttccttcta catcatctcc agagactcca gcctgttgca gcgcctcctt   1080

catcacaatg cccccaagct cagcagcttt cacctctttc aaaactccgc cgaatttgcc   1140

aaatggagtt cttgcagcac ttacaatgac tgttttcctc at                      1182


<210>  131
<211>  393
<212>  PRT
<213>  Bacillus subtilis

<400>  131

Met Arg Lys Thr Val Ile Val Ser Ala Ala Arg Thr Pro Phe Gly Lys 
1               5                   10                  15      


Phe Gly Gly Val Leu Lys Glu Val Lys Ala Ala Glu Leu Gly Gly Ile 
            20                  25                  30          


Val Met Lys Glu Ala Leu Gln Gln Ala Gly Val Ser Gly Asp Asp Val 
        35                  40                  45              


Glu Gly Asn Val Met Gly Met Val Val Gln Ala Gly Ser Gly Gln Ile 
    50                  55                  60                  


Pro Ser Arg Gln Ala Ala Arg Leu Ala Gly Met Pro Trp Ser Val Pro 
65                  70                  75                  80  


Ser Glu Thr Leu Asn Lys Val Cys Ala Ser Gly Leu Arg Ala Val Thr 
                85                  90                  95      


Leu Cys Asp Gln Met Ile Arg Ala Gln Asp Ala Asp Ile Leu Val Ala 
            100                 105                 110         


Gly Gly Met Glu Ser Met Ser Asn Ile Pro Tyr Ala Val Pro Ala Gly 
        115                 120                 125             


Arg Trp Gly Ala Arg Met Gly Asp Gly Glu Leu Arg Asp Leu Met Val 
    130                 135                 140                 


Tyr Asp Gly Leu Thr Cys Ala Phe Asp Glu Val His Met Ala Val His 
145                 150                 155                 160 


Gly Asn Thr Ala Ala Lys Glu Tyr Ala Ile Ser Arg Arg Glu Gln Asp 
                165                 170                 175     


Glu Trp Ala Leu Arg Ser His Ala Arg Ala Ala Lys Ala Ala Asp Glu 
            180                 185                 190         


Gly Lys Phe Gln Asp Glu Ile Val Pro Val Asn Trp Ile Gly Arg Lys 
        195                 200                 205             


Gly Lys Pro Asn Val Val Asp Lys Asp Glu Ala Ile Arg Arg Asp Thr 
    210                 215                 220                 


Ser Leu Asp Gln Leu Ala Lys Leu Ala Pro Ile Tyr Ala Ser Asp Gly 
225                 230                 235                 240 


Ser Ile Thr Ala Gly Asn Ala Pro Gly Val Asn Asp Gly Ala Gly Ala 
                245                 250                 255     


Phe Val Leu Met Ser Glu Glu Lys Ala Ala Glu Leu Gly Lys Arg Pro 
            260                 265                 270         


Leu Ala Thr Ile Leu Gly Phe Ser Thr Thr Gly Met Pro Ala His Glu 
        275                 280                 285             


Leu Ala Ala Ala Pro Gly Phe Ala Ile Asn Lys Leu Leu Lys Lys Asn 
    290                 295                 300                 


Gly Leu Thr Val Gln Asp Ile Asp Leu Phe Glu Val Asn Glu Ala Phe 
305                 310                 315                 320 


Ala Ser Val Val Leu Thr Cys Glu Lys Ile Val Gly Phe Asp Leu Glu 
                325                 330                 335     


Lys Val Asn Val Asn Gly Gly Ala Ile Ala Leu Gly His Pro Ile Gly 
            340                 345                 350         


Ala Ser Gly Ala Arg Ile Leu Met Thr Leu Val Tyr Glu Leu Lys Arg 
        355                 360                 365             


Arg Gly Gly Gly Leu Gly Val Ala Ala Ile Cys Ser Gly Ala Ala Gln 
    370                 375                 380                 


Gly Asp Ala Val Leu Val Gln Val His 
385                 390             


<210>  132
<211>  1197
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  132
atgtctcaga acgtttacat tgtatcgact gccagaaccc caattggttc attccagggt     60

tctctatcct ccaagacagc agtggaattg ggtgctgttg ctttaaaagg cgccttggct    120

aaggttccag aattggatgc atccaaggat tttgacgaaa ttatttttgg taacgttctt    180

tctgccaatt tgggccaagc tccggccaga caagttgctt tggctgccgg tttgagtaat    240

catatcgttg caagcacagt taacaaggtc tgtgcatccg ctatgaaggc aatcattttg    300

ggtgctcaat ccatcaaatg tggtaatgct gatgttgtcg tagctggtgg ttgtgaatct    360

atgactaacg caccatacta catgccagca gcccgtgcgg gtgccaaatt tggccaaact    420

gttcttgttg atggtgtcga aagagatggg ttgaacgatg cgtacgatgg tctagccatg    480

ggtgtacacg cagaaaagtg tgcccgtgat tgggatatta ctagagaaca acaagacaat    540

tttgccatcg aatcctacca aaaatctcaa aaatctcaaa aggaaggtaa attcgacaat    600

gaaattgtac ctgttaccat taagggattt agaggtaagc ctgatactca agtcacgaag    660

gacgaggaac ctgctagatt acacgttgaa aaattgagat ctgcaaggac tgttttccaa    720

aaagaaaacg gtactgttac tgccgctaac gcttctccaa tcaacgatgg tgctgcagcc    780

gtcatcttgg tttccgaaaa agttttgaag gaaaagaatt tgaagccttt ggctattatc    840

aaaggttggg gtgaggccgc tcatcaacca gctgatttta catgggctcc atctcttgca    900

gttccaaagg ctttgaaaca tgctggcatc gaagacatca attctgttga ttactttgaa    960

ttcaatgaag ccttttcggt tgtcggtttg gtgaacacta agattttgaa gctagaccca   1020

tctaaggtta atgtatatgg tggtgctgtt gctctaggtc acccattggg ttgttctggt   1080

gctagagtgg ttgttacact gctatccatc ttacagcaag aaggaggtaa gatcggtgtt   1140

gccgccattt gtaatggtgg tggtggtgct tcctctattg tcattgaaaa gatatga      1197


<210>  133
<211>  398
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  133

Met Ser Gln Asn Val Tyr Ile Val Ser Thr Ala Arg Thr Pro Ile Gly 
1               5                   10                  15      


Ser Phe Gln Gly Ser Leu Ser Ser Lys Thr Ala Val Glu Leu Gly Ala 
            20                  25                  30          


Val Ala Leu Lys Gly Ala Leu Ala Lys Val Pro Glu Leu Asp Ala Ser 
        35                  40                  45              


Lys Asp Phe Asp Glu Ile Ile Phe Gly Asn Val Leu Ser Ala Asn Leu 
    50                  55                  60                  


Gly Gln Ala Pro Ala Arg Gln Val Ala Leu Ala Ala Gly Leu Ser Asn 
65                  70                  75                  80  


His Ile Val Ala Ser Thr Val Asn Lys Val Cys Ala Ser Ala Met Lys 
                85                  90                  95      


Ala Ile Ile Leu Gly Ala Gln Ser Ile Lys Cys Gly Asn Ala Asp Val 
            100                 105                 110         


Val Val Ala Gly Gly Cys Glu Ser Met Thr Asn Ala Pro Tyr Tyr Met 
        115                 120                 125             


Pro Ala Ala Arg Ala Gly Ala Lys Phe Gly Gln Thr Val Leu Val Asp 
    130                 135                 140                 


Gly Val Glu Arg Asp Gly Leu Asn Asp Ala Tyr Asp Gly Leu Ala Met 
145                 150                 155                 160 


Gly Val His Ala Glu Lys Cys Ala Arg Asp Trp Asp Ile Thr Arg Glu 
                165                 170                 175     


Gln Gln Asp Asn Phe Ala Ile Glu Ser Tyr Gln Lys Ser Gln Lys Ser 
            180                 185                 190         


Gln Lys Glu Gly Lys Phe Asp Asn Glu Ile Val Pro Val Thr Ile Lys 
        195                 200                 205             


Gly Phe Arg Gly Lys Pro Asp Thr Gln Val Thr Lys Asp Glu Glu Pro 
    210                 215                 220                 


Ala Arg Leu His Val Glu Lys Leu Arg Ser Ala Arg Thr Val Phe Gln 
225                 230                 235                 240 


Lys Glu Asn Gly Thr Val Thr Ala Ala Asn Ala Ser Pro Ile Asn Asp 
                245                 250                 255     


Gly Ala Ala Ala Val Ile Leu Val Ser Glu Lys Val Leu Lys Glu Lys 
            260                 265                 270         


Asn Leu Lys Pro Leu Ala Ile Ile Lys Gly Trp Gly Glu Ala Ala His 
        275                 280                 285             


Gln Pro Ala Asp Phe Thr Trp Ala Pro Ser Leu Ala Val Pro Lys Ala 
    290                 295                 300                 


Leu Lys His Ala Gly Ile Glu Asp Ile Asn Ser Val Asp Tyr Phe Glu 
305                 310                 315                 320 


Phe Asn Glu Ala Phe Ser Val Val Gly Leu Val Asn Thr Lys Ile Leu 
                325                 330                 335     


Lys Leu Asp Pro Ser Lys Val Asn Val Tyr Gly Gly Ala Val Ala Leu 
            340                 345                 350         


Gly His Pro Leu Gly Cys Ser Gly Ala Arg Val Val Val Thr Leu Leu 
        355                 360                 365             


Ser Ile Leu Gln Gln Glu Gly Gly Lys Ile Gly Val Ala Ala Ile Cys 
    370                 375                 380                 


Asn Gly Gly Gly Gly Ala Ser Ser Ile Val Ile Glu Lys Ile 
385                 390                 395             


<210>  134
<211>  864
<212>  DNA
<213>  Bacillus subtilis

<400>  134
atggaaatca aacaaatcat ggtagctggc gcaggtcaga tggggagcgg aattgctcaa     60

acagccgccg acgcgggctt ttatgtgcgg atgtatgatg tgaatccaga ggccgcggag    120

gcaggattga aacggctgaa gaaacagctg gcccgtgatg ctgagaaagg aaaaaggacc    180

gagacggaag tgaagagcgt aatcaaccgc atttcgattt ctcaaacact tgaggaggca    240

gagcatgcgg acattgtgat tgaggctatc gcagaaaaca tggcggcaaa aactgagatg    300

tttaaaacac ttgatcgcat ttgcccgcct catacgattt tggccagcaa tacatcttcc    360

ttgcctatta cagaaatcgc tgctgtaaca aaccggcctc aacgggttat tggcatgcat    420

tttatgaatc ccgtccctgt aatgaagctg gtagaagtga ttcgaggctt ggctacatca    480

gaagaaacgg ccttagatgt tatggcatta gcggaaaaga tggggaaaac agcggtagaa    540

gtcaatgatt ttcctgggtt tgtttccaac cgtgtgcttc ttccaatgat taatgaagcc    600

atctattgcg tgtatgaggg agtggcgaag ccggaggcaa tagatgaagt gatgaagctg    660

ggcatgaatc atccgatggg tccgcttgca ttagcggatt ttatcggact ggatacgtgt    720

ttatcaatta tggaagtcct tcactcaggc cttggcgatt ccaaataccg tccttgcccg    780

ctgctccgca agtatgtcaa agcaggctgg cttggcaaaa agagcggacg cggtttttat    840

gactatgagg agaagacttc ctga                                           864


<210>  135
<211>  287
<212>  PRT
<213>  Bacillus subtilis

<400>  135

Met Glu Ile Lys Gln Ile Met Val Ala Gly Ala Gly Gln Met Gly Ser 
1               5                   10                  15      


Gly Ile Ala Gln Thr Ala Ala Asp Ala Gly Phe Tyr Val Arg Met Tyr 
            20                  25                  30          


Asp Val Asn Pro Glu Ala Ala Glu Ala Gly Leu Lys Arg Leu Lys Lys 
        35                  40                  45              


Gln Leu Ala Arg Asp Ala Glu Lys Gly Lys Arg Thr Glu Thr Glu Val 
    50                  55                  60                  


Lys Ser Val Ile Asn Arg Ile Ser Ile Ser Gln Thr Leu Glu Glu Ala 
65                  70                  75                  80  


Glu His Ala Asp Ile Val Ile Glu Ala Ile Ala Glu Asn Met Ala Ala 
                85                  90                  95      


Lys Thr Glu Met Phe Lys Thr Leu Asp Arg Ile Cys Pro Pro His Thr 
            100                 105                 110         


Ile Leu Ala Ser Asn Thr Ser Ser Leu Pro Ile Thr Glu Ile Ala Ala 
        115                 120                 125             


Val Thr Asn Arg Pro Gln Arg Val Ile Gly Met His Phe Met Asn Pro 
    130                 135                 140                 


Val Pro Val Met Lys Leu Val Glu Val Ile Arg Gly Leu Ala Thr Ser 
145                 150                 155                 160 


Glu Glu Thr Ala Leu Asp Val Met Ala Leu Ala Glu Lys Met Gly Lys 
                165                 170                 175     


Thr Ala Val Glu Val Asn Asp Phe Pro Gly Phe Val Ser Asn Arg Val 
            180                 185                 190         


Leu Leu Pro Met Ile Asn Glu Ala Ile Tyr Cys Val Tyr Glu Gly Val 
        195                 200                 205             


Ala Lys Pro Glu Ala Ile Asp Glu Val Met Lys Leu Gly Met Asn His 
    210                 215                 220                 


Pro Met Gly Pro Leu Ala Leu Ala Asp Phe Ile Gly Leu Asp Thr Cys 
225                 230                 235                 240 


Leu Ser Ile Met Glu Val Leu His Ser Gly Leu Gly Asp Ser Lys Tyr 
                245                 250                 255     


Arg Pro Cys Pro Leu Leu Arg Lys Tyr Val Lys Ala Gly Trp Leu Gly 
            260                 265                 270         


Lys Lys Ser Gly Arg Gly Phe Tyr Asp Tyr Glu Glu Lys Thr Ser 
        275                 280                 285         


<210>  136
<211>  855
<212>  DNA
<213>  Ralstonia eutropha

<400>  136
atggcaatca ggacagtggg catcgtgggt gccggcacca tgggcaacgg catcgcgcag     60

gcttgtgcgg tggtgggcct ggacgtggtg atggtggata tcagcgacgc agcggtgcag    120

aagggcatcg ccaccgtcgc cggcagcctg gaccgcctga tcaagaagga caagatcagc    180

gaagccgaca agatgactgc gctcgcgcgc atccacggca gcaccgcgta tgacgacctg    240

aagaaggccg atatcgtgat cgaggccgcc accgagaact ttgacctgaa ggtcaagatc    300

ctcaagcaga tcgacagcat cgtcggcgag aacgtcatca ttgcttcgaa cacgtcgtcg    360

atctcgatca ccaagctggc cgccgtgacg agtcgccccg agcgcttcat cggcatgcac    420

ttcttcaacc cggtgccggt gatggcgctg gtggaactga tccgcggcct gcagaccagc    480

gacgcggctc acgccgatgt cgaggcgctg gccaaggaac tgggcaagta cccgatcacc    540

gtcaagaaca gcccgggctt cgtcgtcaac cgcatcctgt gcccgatgat caacgaagcc    600

ttctgcgtgc tcggtgaagg cctggcctcg ccggaagaga tcgacgaagg catgaagctc    660

ggctgcaacc atccgatcgg ccccctggca ctggccgaca tgatcggcct ggacaccatg    720

ctggcagtga tggaagtgct gtacacagaa tttgccgacc cgaagtatcg tccggccatg    780

ctgatgcgcg agatggtggc tgcggggtat ctgggccgca agactggccg tggcgtgtac    840

gtctacagca agtaa                                                     855


<210>  137
<211>  284
<212>  PRT
<213>  Ralstonia eutropha

<400>  137

Met Ala Ile Arg Thr Val Gly Ile Val Gly Ala Gly Thr Met Gly Asn 
1               5                   10                  15      


Gly Ile Ala Gln Ala Cys Ala Val Val Gly Leu Asp Val Val Met Val 
            20                  25                  30          


Asp Ile Ser Asp Ala Ala Val Gln Lys Gly Ile Ala Thr Val Ala Gly 
        35                  40                  45              


Ser Leu Asp Arg Leu Ile Lys Lys Asp Lys Ile Ser Glu Ala Asp Lys 
    50                  55                  60                  


Met Thr Ala Leu Ala Arg Ile His Gly Ser Thr Ala Tyr Asp Asp Leu 
65                  70                  75                  80  


Lys Lys Ala Asp Ile Val Ile Glu Ala Ala Thr Glu Asn Phe Asp Leu 
                85                  90                  95      


Lys Val Lys Ile Leu Lys Gln Ile Asp Ser Ile Val Gly Glu Asn Val 
            100                 105                 110         


Ile Ile Ala Ser Asn Thr Ser Ser Ile Ser Ile Thr Lys Leu Ala Ala 
        115                 120                 125             


Val Thr Ser Arg Pro Glu Arg Phe Ile Gly Met His Phe Phe Asn Pro 
    130                 135                 140                 


Val Pro Val Met Ala Leu Val Glu Leu Ile Arg Gly Leu Gln Thr Ser 
145                 150                 155                 160 


Asp Ala Ala His Ala Asp Val Glu Ala Leu Ala Lys Glu Leu Gly Lys 
                165                 170                 175     


Tyr Pro Ile Thr Val Lys Asn Ser Pro Gly Phe Val Val Asn Arg Ile 
            180                 185                 190         


Leu Cys Pro Met Ile Asn Glu Ala Phe Cys Val Leu Gly Glu Gly Leu 
        195                 200                 205             


Ala Ser Pro Glu Glu Ile Asp Glu Gly Met Lys Leu Gly Cys Asn His 
    210                 215                 220                 


Pro Ile Gly Pro Leu Ala Leu Ala Asp Met Ile Gly Leu Asp Thr Met 
225                 230                 235                 240 


Leu Ala Val Met Glu Val Leu Tyr Thr Glu Phe Ala Asp Pro Lys Tyr 
                245                 250                 255     


Arg Pro Ala Met Leu Met Arg Glu Met Val Ala Ala Gly Tyr Leu Gly 
            260                 265                 270         


Arg Lys Thr Gly Arg Gly Val Tyr Val Tyr Ser Lys 
        275                 280                 


<210>  138
<211>  741
<212>  DNA
<213>  Alcaligenes eutrophis

<400>  138
atgactcagc gcattgcgta tgtgaccggc ggcatgggtg gtatcggaac cgccatttgc     60

cagcggctgg ccaaggatgg ctttcgtgtg gtggccggtt gcggccccaa ctcgccgcgc    120

cgcgaaaagt ggctggagca gcagaaggcc ctgggcttcg atttcattgc ctcggaaggc    180

aatgtggctg actgggactc gaccaagacc gcattcgaca aggtcaagtc cgaggtcggc    240

gaggttgatg tgctgatcaa caacgccggt atcacccgcg acgtggtgtt ccgcaagatg    300

acccgcgccg actgggatgc ggtgatcgac accaacctga cctcgctgtt caacgtcacc    360

aagcaggtga tcgacggcat ggccgaccgt ggctggggcc gcatcgtcaa catctcgtcg    420

gtgaacgggc agaagggcca gttcggccag accaactact ccaccgccaa ggccggcctg    480

catggcttca ccatggcact ggcgcaggaa gtggcgacca agggcgtgac cgtcaacacg    540

gtctctccgg gctatatcgc caccgacatg gtcaaggcga tccgccagga cgtgctcgac    600

aagatcgtcg cgacgatccc ggtcaagcgc ctgggcctgc cggaagagat cgcctcgatc    660

tgcgcctggt tgtcgtcgga ggagtccggt ttctcgaccg gcgccgactt ctcgctcaac    720

ggcggcctgc atatgggctg a                                              741


<210>  139
<211>  246
<212>  PRT
<213>  Alcaligenes eutrophus

<400>  139

Met Thr Gln Arg Ile Ala Tyr Val Thr Gly Gly Met Gly Gly Ile Gly 
1               5                   10                  15      


Thr Ala Ile Cys Gln Arg Leu Ala Lys Asp Gly Phe Arg Val Val Ala 
            20                  25                  30          


Gly Cys Gly Pro Asn Ser Pro Arg Arg Glu Lys Trp Leu Glu Gln Gln 
        35                  40                  45              


Lys Ala Leu Gly Phe Asp Phe Ile Ala Ser Glu Gly Asn Val Ala Asp 
    50                  55                  60                  


Trp Asp Ser Thr Lys Thr Ala Phe Asp Lys Val Lys Ser Glu Val Gly 
65                  70                  75                  80  


Glu Val Asp Val Leu Ile Asn Asn Ala Gly Ile Thr Arg Asp Val Val 
                85                  90                  95      


Phe Arg Lys Met Thr Arg Ala Asp Trp Asp Ala Val Ile Asp Thr Asn 
            100                 105                 110         


Leu Thr Ser Leu Phe Asn Val Thr Lys Gln Val Ile Asp Gly Met Ala 
        115                 120                 125             


Asp Arg Gly Trp Gly Arg Ile Val Asn Ile Ser Ser Val Asn Gly Gln 
    130                 135                 140                 


Lys Gly Gln Phe Gly Gln Thr Asn Tyr Ser Thr Ala Lys Ala Gly Leu 
145                 150                 155                 160 


His Gly Phe Thr Met Ala Leu Ala Gln Glu Val Ala Thr Lys Gly Val 
                165                 170                 175     


Thr Val Asn Thr Val Ser Pro Gly Tyr Ile Ala Thr Asp Met Val Lys 
            180                 185                 190         


Ala Ile Arg Gln Asp Val Leu Asp Lys Ile Val Ala Thr Ile Pro Val 
        195                 200                 205             


Lys Arg Leu Gly Leu Pro Glu Glu Ile Ala Ser Ile Cys Ala Trp Leu 
    210                 215                 220                 


Ser Ser Glu Glu Ser Gly Phe Ser Thr Gly Ala Asp Phe Ser Leu Asn 
225                 230                 235                 240 


Gly Gly Leu His Met Gly 
                245     


<210>  140
<211>  768
<212>  DNA
<213>  Escherichia coli

<400>  140
atgagcgaac tgatcgtcag ccgtcagcaa cgagtattgt tgctgaccct taaccgtccc     60

gccgcacgta atgcgctaaa taatgccctg ctgatgcaac tggtaaatga actggaagct    120

gcggctaccg ataccagcat ttcggtctgt gtgattaccg gtaatgcacg cttttttgcc    180

gctggggccg atctcaacga aatggcagaa aaagatctcg cggccacctt aaacgataca    240

cgtccgcagc tatgggcgcg attgcaggcc tttaataaac cacttatcgc agccgtcaat    300

ggttacgcgc ttggggcggg ttgcgaactg gcattgttgt gcgatgtggt ggttgccgga    360

gagaacgcgc gttttgggtt gccggaaatc actctcggca tcatgcctgg cgcaggcgga    420

acgcaacgtt taatccgtag tgtcggtaaa tcgttagcca gcaaaatggt gctgagcgga    480

gaaagtatca ccgctcagca agcacagcag gccgggctgg ttagcgacgt cttccccagc    540

gatttaaccc tcgaatacgc cttacagctg gcatcgaaaa tggcacgtca ctcgccgctg    600

gccttacaag cggcaaagca agcgctgcgc cagtcgcagg aagtggcttt gcaagccgga    660

cttgcccagg agcgacagtt attcaccttg ctggcggcaa cagaagatcg tcatgaaggc    720

atctccgctt tcttacaaaa acgcacgccc gactttaaag gacgctaa                 768


<210>  141
<211>  255
<212>  PRT
<213>  Escherichia coli

<400>  141

Met Ser Glu Leu Ile Val Ser Arg Gln Gln Arg Val Leu Leu Leu Thr 
1               5                   10                  15      


Leu Asn Arg Pro Ala Ala Arg Asn Ala Leu Asn Asn Ala Leu Leu Met 
            20                  25                  30          


Gln Leu Val Asn Glu Leu Glu Ala Ala Ala Thr Asp Thr Ser Ile Ser 
        35                  40                  45              


Val Cys Val Ile Thr Gly Asn Ala Arg Phe Phe Ala Ala Gly Ala Asp 
    50                  55                  60                  


Leu Asn Glu Met Ala Glu Lys Asp Leu Ala Ala Thr Leu Asn Asp Thr 
65                  70                  75                  80  


Arg Pro Gln Leu Trp Ala Arg Leu Gln Ala Phe Asn Lys Pro Leu Ile 
                85                  90                  95      


Ala Ala Val Asn Gly Tyr Ala Leu Gly Ala Gly Cys Glu Leu Ala Leu 
            100                 105                 110         


Leu Cys Asp Val Val Val Ala Gly Glu Asn Ala Arg Phe Gly Leu Pro 
        115                 120                 125             


Glu Ile Thr Leu Gly Ile Met Pro Gly Ala Gly Gly Thr Gln Arg Leu 
    130                 135                 140                 


Ile Arg Ser Val Gly Lys Ser Leu Ala Ser Lys Met Val Leu Ser Gly 
145                 150                 155                 160 


Glu Ser Ile Thr Ala Gln Gln Ala Gln Gln Ala Gly Leu Val Ser Asp 
                165                 170                 175     


Val Phe Pro Ser Asp Leu Thr Leu Glu Tyr Ala Leu Gln Leu Ala Ser 
            180                 185                 190         


Lys Met Ala Arg His Ser Pro Leu Ala Leu Gln Ala Ala Lys Gln Ala 
        195                 200                 205             


Leu Arg Gln Ser Gln Glu Val Ala Leu Gln Ala Gly Leu Ala Gln Glu 
    210                 215                 220                 


Arg Gln Leu Phe Thr Leu Leu Ala Ala Thr Glu Asp Arg His Glu Gly 
225                 230                 235                 240 


Ile Ser Ala Phe Leu Gln Lys Arg Thr Pro Asp Phe Lys Gly Arg 
                245                 250                 255 


<210>  142
<211>  783
<212>  DNA
<213>  Bacillus subtilis

<400>  142
atgggagatt ctattctttt tactgttaaa aatgaacata tggcgttgat caccttaaac     60

aggcctcagg cagcaaatgc tctttcagcg gaaatgctta gaaacctgca aatgattatc    120

caggaaattg aatttaactc aaacatccgt tgcgtcatcc tcacaggcac cggtgaaaaa    180

gcgttttgtg caggggcaga cctgaaggaa cggataaaac tgaaagaaga tcaggttctg    240

gaaagtgtat ctctcattca aagaacggcg gctttacttg atgccttgcc gcagccggtc    300

atagctgcga taaatggaag cgcattaggc ggcggactag aattggcatt ggcatgcgac    360

cttcgaatcg caactgaagc agctgtgctg ggacttccgg aaacagggtt agctattatc    420

ccgggcgctg gagggaccca aaggctgccc cggctgattg gcagaggaaa agcaaaagaa    480

ttcatttata caggcagacg cgtgaccgca cacgaagcaa aagaaatcgg ccttgtagag    540

catgtcacgg ctccttgtga ccttatgcca aaagcagagg aactggccgc agccatttct    600

gccaacggac cgatcgctgt ccgtcaggct aaatttgcaa tcaataaagg attggagaca    660

gatcttgcta caggccttgc gattgaacaa aaagcgtatg aacaaaccat cccgacaaaa    720

gacaggagag aagggcttca ggcctttcaa gaaaaaagac gggccgtata caagggaata    780

taa                                                                  783


<210>  143
<211>  260
<212>  PRT
<213>  Bacillus subtilis

<400>  143

Met Gly Asp Ser Ile Leu Phe Thr Val Lys Asn Glu His Met Ala Leu 
1               5                   10                  15      


Ile Thr Leu Asn Arg Pro Gln Ala Ala Asn Ala Leu Ser Ala Glu Met 
            20                  25                  30          


Leu Arg Asn Leu Gln Met Ile Ile Gln Glu Ile Glu Phe Asn Ser Asn 
        35                  40                  45              


Ile Arg Cys Val Ile Leu Thr Gly Thr Gly Glu Lys Ala Phe Cys Ala 
    50                  55                  60                  


Gly Ala Asp Leu Lys Glu Arg Ile Lys Leu Lys Glu Asp Gln Val Leu 
65                  70                  75                  80  


Glu Ser Val Ser Leu Ile Gln Arg Thr Ala Ala Leu Leu Asp Ala Leu 
                85                  90                  95      


Pro Gln Pro Val Ile Ala Ala Ile Asn Gly Ser Ala Leu Gly Gly Gly 
            100                 105                 110         


Leu Glu Leu Ala Leu Ala Cys Asp Leu Arg Ile Ala Thr Glu Ala Ala 
        115                 120                 125             


Val Leu Gly Leu Pro Glu Thr Gly Leu Ala Ile Ile Pro Gly Ala Gly 
    130                 135                 140                 


Gly Thr Gln Arg Leu Pro Arg Leu Ile Gly Arg Gly Lys Ala Lys Glu 
145                 150                 155                 160 


Phe Ile Tyr Thr Gly Arg Arg Val Thr Ala His Glu Ala Lys Glu Ile 
                165                 170                 175     


Gly Leu Val Glu His Val Thr Ala Pro Cys Asp Leu Met Pro Lys Ala 
            180                 185                 190         


Glu Glu Leu Ala Ala Ala Ile Ser Ala Asn Gly Pro Ile Ala Val Arg 
        195                 200                 205             


Gln Ala Lys Phe Ala Ile Asn Lys Gly Leu Glu Thr Asp Leu Ala Thr 
    210                 215                 220                 


Gly Leu Ala Ile Glu Gln Lys Ala Tyr Glu Gln Thr Ile Pro Thr Lys 
225                 230                 235                 240 


Asp Arg Arg Glu Gly Leu Gln Ala Phe Gln Glu Lys Arg Arg Ala Val 
                245                 250                 255     


Tyr Lys Gly Ile 
            260 


<210>  144
<211>  405
<212>  DNA
<213>  Aeromonas caviae

<400>  144
atgagcgcac aatccctgga agtaggccag aaggcccgtc tcagcaagcg gttcggggcg     60

gcggaggtag ccgccttcgc cgcgctctcg gaggacttca accccctgca cctggacccg    120

gccttcgccg ccaccacggc gttcgagcgg cccatagtcc acggcatgct gctcgccagc    180

ctcttctccg ggctgctggg ccagcagttg ccgggcaagg ggagcatcta tctgggtcaa    240

agcctcagct tcaagctgcc ggtctttgtc ggggacgagg tgacggccga ggtggaggtg    300

accgcccttc gcgaggacaa gcccatcgcc accctgacca cccgcatctt cacccaaggc    360

ggcgccctcg ccgtgacggg ggaagccgtg gtcaagctgc cttaa                    405


<210>  145
<211>  134
<212>  PRT
<213>  Aeromonas caviae

<400>  145

Met Ser Ala Gln Ser Leu Glu Val Gly Gln Lys Ala Arg Leu Ser Lys 
1               5                   10                  15      


Arg Phe Gly Ala Ala Glu Val Ala Ala Phe Ala Ala Leu Ser Glu Asp 
            20                  25                  30          


Phe Asn Pro Leu His Leu Asp Pro Ala Phe Ala Ala Thr Thr Ala Phe 
        35                  40                  45              


Glu Arg Pro Ile Val His Gly Met Leu Leu Ala Ser Leu Phe Ser Gly 
    50                  55                  60                  


Leu Leu Gly Gln Gln Leu Pro Gly Lys Gly Ser Ile Tyr Leu Gly Gln 
65                  70                  75                  80  


Ser Leu Ser Phe Lys Leu Pro Val Phe Val Gly Asp Glu Val Thr Ala 
                85                  90                  95      


Glu Val Glu Val Thr Ala Leu Arg Glu Asp Lys Pro Ile Ala Thr Leu 
            100                 105                 110         


Thr Thr Arg Ile Phe Thr Gln Gly Gly Ala Leu Ala Val Thr Gly Glu 
        115                 120                 125             


Ala Val Val Lys Leu Pro 
    130                 


<210>  146
<211>  1912
<212>  DNA
<213>  Euglena gracilis

<400>  146
ttttcgcccg tgcaccacga tgtcgtgccc cgcctcgccg tctgctgccg tggtgtctgc     60

cggcgccctc tgcctgtgcg tggcaacggt attgttggcg actggatcca accccaccgc    120

cctgtccact gcttccactc gctctccgac ctcactggtc cgtggggtgg acaggggctt    180

gatgaggcca accactgcag cggctctgac gacaatgaga gaggtgcccc agatggctga    240

gggattttca ggcgaagcca cgtctgcatg ggccgccgcg gggccgcagt gggcggcgcc    300

gctcgtggcc gcggcctcct ccgcactggc gctgtggtgg tgggccgccc ggcgcagcgt    360

gcggcggccg ctggcagcgc tggcggagct gcccaccgcg gtcacccacc tggccccccc    420

gatggcgatg ttcaccacca cagcgaaggt catccagccc aagattcgtg gcttcatctg    480

cacgaccacc cacccgatcg gctgtgagaa gcgggtccag gaggagatcg cgtacgcccg    540

tgcccacccg cccaccagcc ctggcccgaa gagggtgctg gtcatcggct gcagtaccgg    600

ctacgggctc tccacccgca tcaccgctgc cttcggctac caggccgcca cgctgggcgt    660

gttcctggcg ggccccccga cgaagggccg ccccgccgcg gcgggctggt acaacaccgt    720

ggcgttcgag aaggccgccc tggaggccgg gctgtacgcc cggagcctta atggcgacgc    780

cttcgactcc acaacgaagg cgcggacggt cgaggcgatc aagcgggacc tcggcacggt    840

ggacctcgtg gtgtacagca tcgccgcccc gaagcggacg gaccctgcca ccggcgtcct    900

ccacaaggcc tgcctgaagc ccatcggcgc cacgtacacc aaccgcactg tgaacaccga    960

caaggcggag gtgaccgacg tcagcattga gccggcctcc cccgaagaga tcgcggacac   1020

ggtgaaggtg atgggcgggg aggactggga gctctggatc caggcgctgt cggaggccgg   1080

cgtgctggcg gagggggcca agacggtggc gtactcctac atcggccccg agatgacgtg   1140

gcctgtctac tggtccggca ccatcgggga ggccaagaag gacgtggaga aggctgccaa   1200

gcgcatcacg cagcagtacg gctgcccggc gtacccggtg gtggccaagg ccttggtcac   1260

ccaggccagc tccgccatcc cggtggtgcc gctctacatc tgcctgctgt accgcgttat   1320

gaaggagaag ggcacccacg agggctgcat cgagcagatg gtgcggctgc tcaccacgaa   1380

gctgtacccc gagaacgggg cccccatcgt cgatgaggcc ggacgtgtgc gggtggatga   1440

ctgggagatg gcggaggatg tgcagcaggc tgttaaggac ctctggagcc aggtgagcac   1500

tgccaacctc aaggacatct ccgacttcgc tgggtatcaa actgagttcc tgcggctgtt   1560

cgggttcggc attgacggcg tggactacga ccagcccgtg gacgtggagg cggacctccc   1620

cagtgctgcc cagcagtagg tgctggacgc cgcctctctc cggggggtct gccaaaatgg   1680

tcgctccccc aacccaaccc cctgcccacc atcggggtcc cgcgggtgaa tgcggccccc   1740

acccaaaggc aaaggtcaag gccggggccc caccgccaaa gggtaacaca tatgtatccg   1800

tcgggggctg atccgcgtgc gacacgggcc ataattgtgc cccacgggat gtccatgcgc   1860

ctaagacaac tgccccggcc gacagtcgct accgccttga gttccccagg ca           1912


<210>  147
<211>  539
<212>  PRT
<213>  Euglena gracilis

<400>  147

Met Ser Cys Pro Ala Ser Pro Ser Ala Ala Val Val Ser Ala Gly Ala 
1               5                   10                  15      


Leu Cys Leu Cys Val Ala Thr Val Leu Leu Ala Thr Gly Ser Asn Pro 
            20                  25                  30          


Thr Ala Leu Ser Thr Ala Ser Thr Arg Ser Pro Thr Ser Leu Val Arg 
        35                  40                  45              


Gly Val Asp Arg Gly Leu Met Arg Pro Thr Thr Ala Ala Ala Leu Thr 
    50                  55                  60                  


Thr Met Arg Glu Val Pro Gln Met Ala Glu Gly Phe Ser Gly Glu Ala 
65                  70                  75                  80  


Thr Ser Ala Trp Ala Ala Ala Gly Pro Gln Trp Ala Ala Pro Leu Val 
                85                  90                  95      


Ala Ala Ala Ser Ser Ala Leu Ala Leu Trp Trp Trp Ala Ala Arg Arg 
            100                 105                 110         


Ser Val Arg Arg Pro Leu Ala Ala Leu Ala Glu Leu Pro Thr Ala Val 
        115                 120                 125             


Thr His Leu Ala Pro Pro Met Ala Met Phe Thr Thr Thr Ala Lys Val 
    130                 135                 140                 


Ile Gln Pro Lys Ile Arg Gly Phe Ile Cys Thr Thr Thr His Pro Ile 
145                 150                 155                 160 


Gly Cys Glu Lys Arg Val Gln Glu Glu Ile Ala Tyr Ala Arg Ala His 
                165                 170                 175     


Pro Pro Thr Ser Pro Gly Pro Lys Arg Val Leu Val Ile Gly Cys Ser 
            180                 185                 190         


Thr Gly Tyr Gly Leu Ser Thr Arg Ile Thr Ala Ala Phe Gly Tyr Gln 
        195                 200                 205             


Ala Ala Thr Leu Gly Val Phe Leu Ala Gly Pro Pro Thr Lys Gly Arg 
    210                 215                 220                 


Pro Ala Ala Ala Gly Trp Tyr Asn Thr Val Ala Phe Glu Lys Ala Ala 
225                 230                 235                 240 


Leu Glu Ala Gly Leu Tyr Ala Arg Ser Leu Asn Gly Asp Ala Phe Asp 
                245                 250                 255     


Ser Thr Thr Lys Ala Arg Thr Val Glu Ala Ile Lys Arg Asp Leu Gly 
            260                 265                 270         


Thr Val Asp Leu Val Val Tyr Ser Ile Ala Ala Pro Lys Arg Thr Asp 
        275                 280                 285             


Pro Ala Thr Gly Val Leu His Lys Ala Cys Leu Lys Pro Ile Gly Ala 
    290                 295                 300                 


Thr Tyr Thr Asn Arg Thr Val Asn Thr Asp Lys Ala Glu Val Thr Asp 
305                 310                 315                 320 


Val Ser Ile Glu Pro Ala Ser Pro Glu Glu Ile Ala Asp Thr Val Lys 
                325                 330                 335     


Val Met Gly Gly Glu Asp Trp Glu Leu Trp Ile Gln Ala Leu Ser Glu 
            340                 345                 350         


Ala Gly Val Leu Ala Glu Gly Ala Lys Thr Val Ala Tyr Ser Tyr Ile 
        355                 360                 365             


Gly Pro Glu Met Thr Trp Pro Val Tyr Trp Ser Gly Thr Ile Gly Glu 
    370                 375                 380                 


Ala Lys Lys Asp Val Glu Lys Ala Ala Lys Arg Ile Thr Gln Gln Tyr 
385                 390                 395                 400 


Gly Cys Pro Ala Tyr Pro Val Val Ala Lys Ala Leu Val Thr Gln Ala 
                405                 410                 415     


Ser Ser Ala Ile Pro Val Val Pro Leu Tyr Ile Cys Leu Leu Tyr Arg 
            420                 425                 430         


Val Met Lys Glu Lys Gly Thr His Glu Gly Cys Ile Glu Gln Met Val 
        435                 440                 445             


Arg Leu Leu Thr Thr Lys Leu Tyr Pro Glu Asn Gly Ala Pro Ile Val 
    450                 455                 460                 


Asp Glu Ala Gly Arg Val Arg Val Asp Asp Trp Glu Met Ala Glu Asp 
465                 470                 475                 480 


Val Gln Gln Ala Val Lys Asp Leu Trp Ser Gln Val Ser Thr Ala Asn 
                485                 490                 495     


Leu Lys Asp Ile Ser Asp Phe Ala Gly Tyr Gln Thr Glu Phe Leu Arg 
            500                 505                 510         


Leu Phe Gly Phe Gly Ile Asp Gly Val Asp Tyr Asp Gln Pro Val Asp 
        515                 520                 525             


Val Glu Ala Asp Leu Pro Ser Ala Ala Gln Gln 
    530                 535                 


<210>  148
<211>  1344
<212>  DNA
<213>  Sreptomyces collinus

<400>  148
gtgaccgtga aggacatcct ggacgcgatc cagtcgaagg acgccacgtc cgccgacttc     60

gccgccctgc agctccccga gtcgtaccgt gcgatcaccg tgcacaagga cgagacggag    120

atgttcgcgg gtctggagac ccgcgacaag gacccgcgca agtcgatcca cctcgacgag    180

gtgcccgtgc ccgaactggg cccgggcgaa gccctggtgg ccgtcatggc ctcctcggtc    240

aactacaact cggtgtggac ctcgatcttc gagccggtgt cgacgttcgc cttcctggag    300

cgctacggca agctgtcgcc gctgaccaag cgccacgacc tgccgtacca catcatcggc    360

tccgacctcg cgggcgtcgt cctgcgcacc ggccccggcg tcaacgcctg gcagcccggt    420

gacgaggtcg tcgcgcactg cctgagcgtc gagctggagt cgcccgacgg ccacgacgac    480

accatgctcg accccgagca gcgcatctgg ggcttcgaga ccaacttcgg cggcctcgcg    540

gagatcgcgc tggtcaagac gaaccagctg atgccgaagc cgaagcacct cacctgggag    600

gaggccgcgg ccccgggcct ggtgaactcc accgcctacc gccagctggt ctcccgcaac    660

ggcgccgcca tgaagcaggg cgacaacgtc ctgatctggg gcgcgagcgg cgggctcggc    720

tcgtacgcca cgcagttcgc gctcgcgggc ggtgccaacc cgatctgtgt cgtctcctcg    780

ccccagaagg cggagatctg ccgctcgatg ggcgccgagg cgatcatcga ccgcaacgcc    840

gagggctaca agttctggaa ggacgagcac acccaggacc ccaaggagtg gaagcgcttc    900

ggcaagcgca tccgcgagct gaccggcggc gaggacatcg acatcgtctt cgagcacccc    960

ggccgcgaga ccttcggcgc ctccgtctac gtcacccgca agggcggcac catcaccacc   1020

tgcgcctcga cctcgggcta catgcacgag tacgacaacc ggtacctgtg gatgtccctg   1080

aagcggatca tcggctcgca cttcgccaac taccgcgagg cgtacgaggc caaccgcctg   1140

atcgccaagg gcaagatcca cccgacgctg tcgaagacgt actccctgga ggagaccggc   1200

caggcggcgt acgacgtcca ccgcaacctg caccagggca aggtcggcgt cctgtgcctc   1260

gcgccggagg aaggcctcgg cgtgcgcgac gcggagatgc gcgcccagca catcgacgcc   1320

atcaaccgct tccgcaacgt ctga                                          1344


<210>  149
<211>  447
<212>  PRT
<213>  Streptomyces collinus

<400>  149

Met Thr Val Lys Asp Ile Leu Asp Ala Ile Gln Ser Lys Asp Ala Thr 
1               5                   10                  15      


Ser Ala Asp Phe Ala Ala Leu Gln Leu Pro Glu Ser Tyr Arg Ala Ile 
            20                  25                  30          


Thr Val His Lys Asp Glu Thr Glu Met Phe Ala Gly Leu Glu Thr Arg 
        35                  40                  45              


Asp Lys Asp Pro Arg Lys Ser Ile His Leu Asp Glu Val Pro Val Pro 
    50                  55                  60                  


Glu Leu Gly Pro Gly Glu Ala Leu Val Ala Val Met Ala Ser Ser Val 
65                  70                  75                  80  


Asn Tyr Asn Ser Val Trp Thr Ser Ile Phe Glu Pro Val Ser Thr Phe 
                85                  90                  95      


Ala Phe Leu Glu Arg Tyr Gly Lys Leu Ser Pro Leu Thr Lys Arg His 
            100                 105                 110         


Asp Leu Pro Tyr His Ile Ile Gly Ser Asp Leu Ala Gly Val Val Leu 
        115                 120                 125             


Arg Thr Gly Pro Gly Val Asn Ala Trp Gln Pro Gly Asp Glu Val Val 
    130                 135                 140                 


Ala His Cys Leu Ser Val Glu Leu Glu Ser Pro Asp Gly His Asp Asp 
145                 150                 155                 160 


Thr Met Leu Asp Pro Glu Gln Arg Ile Trp Gly Phe Glu Thr Asn Phe 
                165                 170                 175     


Gly Gly Leu Ala Glu Ile Ala Leu Val Lys Thr Asn Gln Leu Met Pro 
            180                 185                 190         


Lys Pro Lys His Leu Thr Trp Glu Glu Ala Ala Ala Pro Gly Leu Val 
        195                 200                 205             


Asn Ser Thr Ala Tyr Arg Gln Leu Val Ser Arg Asn Gly Ala Ala Met 
    210                 215                 220                 


Lys Gln Gly Asp Asn Val Leu Ile Trp Gly Ala Ser Gly Gly Leu Gly 
225                 230                 235                 240 


Ser Tyr Ala Thr Gln Phe Ala Leu Ala Gly Gly Ala Asn Pro Ile Cys 
                245                 250                 255     


Val Val Ser Ser Pro Gln Lys Ala Glu Ile Cys Arg Ser Met Gly Ala 
            260                 265                 270         


Glu Ala Ile Ile Asp Arg Asn Ala Glu Gly Tyr Lys Phe Trp Lys Asp 
        275                 280                 285             


Glu His Thr Gln Asp Pro Lys Glu Trp Lys Arg Phe Gly Lys Arg Ile 
    290                 295                 300                 


Arg Glu Leu Thr Gly Gly Glu Asp Ile Asp Ile Val Phe Glu His Pro 
305                 310                 315                 320 


Gly Arg Glu Thr Phe Gly Ala Ser Val Tyr Val Thr Arg Lys Gly Gly 
                325                 330                 335     


Thr Ile Thr Thr Cys Ala Ser Thr Ser Gly Tyr Met His Glu Tyr Asp 
            340                 345                 350         


Asn Arg Tyr Leu Trp Met Ser Leu Lys Arg Ile Ile Gly Ser His Phe 
        355                 360                 365             


Ala Asn Tyr Arg Glu Ala Tyr Glu Ala Asn Arg Leu Ile Ala Lys Gly 
    370                 375                 380                 


Lys Ile His Pro Thr Leu Ser Lys Thr Tyr Ser Leu Glu Glu Thr Gly 
385                 390                 395                 400 


Gln Ala Ala Tyr Asp Val His Arg Asn Leu His Gln Gly Lys Val Gly 
                405                 410                 415     


Val Leu Cys Leu Ala Pro Glu Glu Gly Leu Gly Val Arg Asp Ala Glu 
            420                 425                 430         


Met Arg Ala Gln His Ile Asp Ala Ile Asn Arg Phe Arg Asn Val 
        435                 440                 445         


<210>  150
<211>  1344
<212>  DNA
<213>  Streptomyces coelicolor

<400>  150
gtgaccgtga aggacatcct ggacgcgatc cagtcgcccg actccacgcc ggccgacatc     60

gccgcactgc cgctccccga gtcgtaccgc gcgatcaccg tgcacaagga cgagaccgag    120

atgttcgcgg gcctcgagac ccgcgacaag gacccccgca agtcgatcca cctggacgac    180

gtgccggtgc ccgagctggg ccccggcgag gccctggtgg ccgtcatggc ctcctcggtc    240

aactacaact cggtgtggac ctcgatcttc gagccgctgt ccaccttcgg gttcctggag    300

cgctacggcc gggtcagcga cctcgccaag cggcacgacc tgccgtacca cgtcatcggc    360

tccgacctcg ccggtgtcgt cctgcgcacc ggtccgggcg tcaacgcctg gcaggcgggc    420

gacgaggtcg tcgcgcactg cctctccgtc gagctggagt cctccgacgg ccacaacgac    480

acgatgctcg accccgagca gcgcatctgg ggcttcgaga ccaacttcgg cggcctcgcg    540

gagatcgcgc tggtcaagtc caaccagctg atgccgaagc cggaccacct gagctgggag    600

gaggccgccg ctcccggcct ggtcaactcc accgcgtacc gccagctcgt ctcccgcaac    660

ggcgccggca tgaagcaggg cgacaacgtg ctcatctggg gcgcgagcgg cggactcggc    720

tcgtacgcca cccagttcgc cctcgccggc ggcgccaacc cgatctgcgt cgtctcctcg    780

ccgcagaagg cggagatctg ccgcgcgatg ggcgccgagg cgatcatcga ccgcaacgcc    840

gagggctacc ggttctggaa ggacgagaac acccaggacc cgaaggagtg gaagcgcttc    900

ggcaagcgca tccgcgaact gaccggcggc gaggacatcg acatcgtctt cgagcacccc    960

ggccgcgaga ccttcggcgc ctccgtcttc gtcacccgca agggcggcac catcaccacc   1020

tgcgcctcga cctcgggcta catgcacgag tacgacaacc gctacctgtg gatgtccctg   1080

aagcgcatca tcggctcgca cttcgccaac taccgcgagg cctgggaggc caaccgcctc   1140

atcgccaagg gcaggatcca ccccacgctc tccaaggtgt actccctcga ggacaccggc   1200

caggccgcct acgacgtcca ccgcaacctc caccagggca aggtcggcgt gctgtgcctg   1260

gcgcccgagg agggcctggg cgtgcgcgac cgggagaagc gcgcgcagca cctcgacgcc   1320

atcaaccgct tccggaacat ctga                                          1344


<210>  151
<211>  447
<212>  PRT
<213>  Streptomyces coelicolor

<400>  151

Met Thr Val Lys Asp Ile Leu Asp Ala Ile Gln Ser Pro Asp Ser Thr 
1               5                   10                  15      


Pro Ala Asp Ile Ala Ala Leu Pro Leu Pro Glu Ser Tyr Arg Ala Ile 
            20                  25                  30          


Thr Val His Lys Asp Glu Thr Glu Met Phe Ala Gly Leu Glu Thr Arg 
        35                  40                  45              


Asp Lys Asp Pro Arg Lys Ser Ile His Leu Asp Asp Val Pro Val Pro 
    50                  55                  60                  


Glu Leu Gly Pro Gly Glu Ala Leu Val Ala Val Met Ala Ser Ser Val 
65                  70                  75                  80  


Asn Tyr Asn Ser Val Trp Thr Ser Ile Phe Glu Pro Leu Ser Thr Phe 
                85                  90                  95      


Gly Phe Leu Glu Arg Tyr Gly Arg Val Ser Asp Leu Ala Lys Arg His 
            100                 105                 110         


Asp Leu Pro Tyr His Val Ile Gly Ser Asp Leu Ala Gly Val Val Leu 
        115                 120                 125             


Arg Thr Gly Pro Gly Val Asn Ala Trp Gln Ala Gly Asp Glu Val Val 
    130                 135                 140                 


Ala His Cys Leu Ser Val Glu Leu Glu Ser Ser Asp Gly His Asn Asp 
145                 150                 155                 160 


Thr Met Leu Asp Pro Glu Gln Arg Ile Trp Gly Phe Glu Thr Asn Phe 
                165                 170                 175     


Gly Gly Leu Ala Glu Ile Ala Leu Val Lys Ser Asn Gln Leu Met Pro 
            180                 185                 190         


Lys Pro Asp His Leu Ser Trp Glu Glu Ala Ala Ala Pro Gly Leu Val 
        195                 200                 205             


Asn Ser Thr Ala Tyr Arg Gln Leu Val Ser Arg Asn Gly Ala Gly Met 
    210                 215                 220                 


Lys Gln Gly Asp Asn Val Leu Ile Trp Gly Ala Ser Gly Gly Leu Gly 
225                 230                 235                 240 


Ser Tyr Ala Thr Gln Phe Ala Leu Ala Gly Gly Ala Asn Pro Ile Cys 
                245                 250                 255     


Val Val Ser Ser Pro Gln Lys Ala Glu Ile Cys Arg Ala Met Gly Ala 
            260                 265                 270         


Glu Ala Ile Ile Asp Arg Asn Ala Glu Gly Tyr Arg Phe Trp Lys Asp 
        275                 280                 285             


Glu Asn Thr Gln Asp Pro Lys Glu Trp Lys Arg Phe Gly Lys Arg Ile 
    290                 295                 300                 


Arg Glu Leu Thr Gly Gly Glu Asp Ile Asp Ile Val Phe Glu His Pro 
305                 310                 315                 320 


Gly Arg Glu Thr Phe Gly Ala Ser Val Phe Val Thr Arg Lys Gly Gly 
                325                 330                 335     


Thr Ile Thr Thr Cys Ala Ser Thr Ser Gly Tyr Met His Glu Tyr Asp 
            340                 345                 350         


Asn Arg Tyr Leu Trp Met Ser Leu Lys Arg Ile Ile Gly Ser His Phe 
        355                 360                 365             


Ala Asn Tyr Arg Glu Ala Trp Glu Ala Asn Arg Leu Ile Ala Lys Gly 
    370                 375                 380                 


Arg Ile His Pro Thr Leu Ser Lys Val Tyr Ser Leu Glu Asp Thr Gly 
385                 390                 395                 400 


Gln Ala Ala Tyr Asp Val His Arg Asn Leu His Gln Gly Lys Val Gly 
                405                 410                 415     


Val Leu Cys Leu Ala Pro Glu Glu Gly Leu Gly Val Arg Asp Arg Glu 
            420                 425                 430         


Lys Arg Ala Gln His Leu Asp Ala Ile Asn Arg Phe Arg Asn Ile 
        435                 440                 445         


<210>  152
<211>  2589
<212>  DNA
<213>  Clostridium acetobutylicum

<400>  152
atgaaagtca caacagtaaa ggaattagat gaaaaactca aggtaattaa agaagctcaa     60

aaaaaattct cttgttactc gcaagaaatg gttgatgaaa tctttagaaa tgcagcaatg    120

gcagcaatcg acgcaaggat agagctagca aaagcagctg ttttggaaac cggtatgggc    180

ttagttgaag acaaggttat aaaaaatcat tttgcaggcg aatacatcta taacaaatat    240

aaggatgaaa aaacctgcgg tataattgaa cgaaatgaac cctacggaat tacaaaaata    300

gcagaaccta taggagttgt agctgctata atccctgtaa caaaccccac atcaacaaca    360

atatttaaat ccttaatatc ccttaaaact agaaatggaa ttttcttttc gcctcaccca    420

agggcaaaaa aatccacaat actagcagct aaaacaatac ttgatgcagc cgttaagagt    480

ggtgccccgg aaaatataat aggttggata gatgaacctt caattgaact aactcaatat    540

ttaatgcaaa aagcagatat aacccttgca actggtggtc cctcactagt taaatctgct    600

tattcttccg gaaaaccagc aataggtgtt ggtccgggta acaccccagt aataattgat    660

gaatctgctc atataaaaat ggcagtaagt tcaattatat tatccaaaac ctatgataat    720

ggtgttatat gtgcttctga acaatctgta atagtcttaa aatccatata taacaaggta    780

aaagatgagt tccaagaaag aggagcttat ataataaaga aaaacgaatt ggataaagtc    840

cgtgaagtga tttttaaaga tggatccgta aaccctaaaa tagtcggaca gtcagcttat    900

actatagcag ctatggctgg cataaaagta cctaaaacca caagaatatt aataggagaa    960

gttacctcct taggtgaaga agaacctttt gcccacgaaa aactatctcc tgttttggct   1020

atgtatgagg ctgacaattt tgatgatgct ttaaaaaaag cagtaactct aataaactta   1080

ggaggcctcg gccatacctc aggaatatat gcagatgaaa taaaagcacg agataaaata   1140

gatagattta gtagtgccat gaaaaccgta agaacctttg taaatatccc aacctcacaa   1200

ggtgcaagtg gagatctata taattttaga ataccacctt ctttcacgct tggctgcgga   1260

ttttggggag gaaattctgt ttccgagaat gttggtccaa aacatctttt gaatattaaa   1320

accgtagctg aaaggagaga aaacatgctt tggtttagag ttccacataa agtatatttt   1380

aagttcggtt gtcttcaatt tgctttaaaa gatttaaaag atctaaagaa aaaaagagcc   1440

tttatagtta ctgatagtga cccctataat ttaaactatg ttgattcaat aataaaaata   1500

cttgagcacc tagatattga ttttaaagta tttaataagg ttggaagaga agctgatctt   1560

aaaaccataa aaaaagcaac tgaagaaatg tcctccttta tgccagacac tataatagct   1620

ttaggtggta cccctgaaat gagctctgca aagctaatgt gggtactata tgaacatcca   1680

gaagtaaaat ttgaagatct tgcaataaaa tttatggaca taagaaagag aatatatact   1740

ttcccaaaac tcggtaaaaa ggctatgtta gttgcaatta caacttctgc tggttccggt   1800

tctgaggtta ctccttttgc tttagtaact gacaataaca ctggaaataa gtacatgtta   1860

gcagattatg aaatgacacc aaatatggca attgtagatg cagaacttat gatgaaaatg   1920

ccaaagggat taaccgctta ttcaggtata gatgcactag taaatagtat agaagcatac   1980

acatccgtat atgcttcaga atacacaaac ggactagcac tagaggcaat acgattaata   2040

tttaaatatt tgcctgaggc ttacaaaaac ggaagaacca atgaaaaagc aagagagaaa   2100

atggctcacg cttcaactat ggcaggtatg gcatccgcta atgcatttct aggtctatgt   2160

cattccatgg caataaaatt aagttcagaa cacaatattc ctagtggcat tgccaatgca   2220

ttactaatag aagaagtaat aaaatttaac gcagttgata atcctgtaaa acaagcccct   2280

tgcccacaat ataagtatcc aaacaccata tttagatatg ctcgaattgc agattatata   2340

aagcttggag gaaatactga tgaggaaaag gtagatctct taattaacaa aatacatgaa   2400

ctaaaaaaag ctttaaatat accaacttca ataaaggatg caggtgtttt ggaggaaaac   2460

ttctattcct cccttgatag aatatctgaa cttgcactag atgatcaatg cacaggcgct   2520

aatcctagat ttcctcttac aagtgagata aaagaaatgt atataaattg ttttaaaaaa   2580

caaccttaa                                                           2589


<210>  153
<211>  862
<212>  PRT
<213>  Clostridium acetobutylicum

<400>  153

Met Lys Val Thr Thr Val Lys Glu Leu Asp Glu Lys Leu Lys Val Ile 
1               5                   10                  15      


Lys Glu Ala Gln Lys Lys Phe Ser Cys Tyr Ser Gln Glu Met Val Asp 
            20                  25                  30          


Glu Ile Phe Arg Asn Ala Ala Met Ala Ala Ile Asp Ala Arg Ile Glu 
        35                  40                  45              


Leu Ala Lys Ala Ala Val Leu Glu Thr Gly Met Gly Leu Val Glu Asp 
    50                  55                  60                  


Lys Val Ile Lys Asn His Phe Ala Gly Glu Tyr Ile Tyr Asn Lys Tyr 
65                  70                  75                  80  


Lys Asp Glu Lys Thr Cys Gly Ile Ile Glu Arg Asn Glu Pro Tyr Gly 
                85                  90                  95      


Ile Thr Lys Ile Ala Glu Pro Ile Gly Val Val Ala Ala Ile Ile Pro 
            100                 105                 110         


Val Thr Asn Pro Thr Ser Thr Thr Ile Phe Lys Ser Leu Ile Ser Leu 
        115                 120                 125             


Lys Thr Arg Asn Gly Ile Phe Phe Ser Pro His Pro Arg Ala Lys Lys 
    130                 135                 140                 


Ser Thr Ile Leu Ala Ala Lys Thr Ile Leu Asp Ala Ala Val Lys Ser 
145                 150                 155                 160 


Gly Ala Pro Glu Asn Ile Ile Gly Trp Ile Asp Glu Pro Ser Ile Glu 
                165                 170                 175     


Leu Thr Gln Tyr Leu Met Gln Lys Ala Asp Ile Thr Leu Ala Thr Gly 
            180                 185                 190         


Gly Pro Ser Leu Val Lys Ser Ala Tyr Ser Ser Gly Lys Pro Ala Ile 
        195                 200                 205             


Gly Val Gly Pro Gly Asn Thr Pro Val Ile Ile Asp Glu Ser Ala His 
    210                 215                 220                 


Ile Lys Met Ala Val Ser Ser Ile Ile Leu Ser Lys Thr Tyr Asp Asn 
225                 230                 235                 240 


Gly Val Ile Cys Ala Ser Glu Gln Ser Val Ile Val Leu Lys Ser Ile 
                245                 250                 255     


Tyr Asn Lys Val Lys Asp Glu Phe Gln Glu Arg Gly Ala Tyr Ile Ile 
            260                 265                 270         


Lys Lys Asn Glu Leu Asp Lys Val Arg Glu Val Ile Phe Lys Asp Gly 
        275                 280                 285             


Ser Val Asn Pro Lys Ile Val Gly Gln Ser Ala Tyr Thr Ile Ala Ala 
    290                 295                 300                 


Met Ala Gly Ile Lys Val Pro Lys Thr Thr Arg Ile Leu Ile Gly Glu 
305                 310                 315                 320 


Val Thr Ser Leu Gly Glu Glu Glu Pro Phe Ala His Glu Lys Leu Ser 
                325                 330                 335     


Pro Val Leu Ala Met Tyr Glu Ala Asp Asn Phe Asp Asp Ala Leu Lys 
            340                 345                 350         


Lys Ala Val Thr Leu Ile Asn Leu Gly Gly Leu Gly His Thr Ser Gly 
        355                 360                 365             


Ile Tyr Ala Asp Glu Ile Lys Ala Arg Asp Lys Ile Asp Arg Phe Ser 
    370                 375                 380                 


Ser Ala Met Lys Thr Val Arg Thr Phe Val Asn Ile Pro Thr Ser Gln 
385                 390                 395                 400 


Gly Ala Ser Gly Asp Leu Tyr Asn Phe Arg Ile Pro Pro Ser Phe Thr 
                405                 410                 415     


Leu Gly Cys Gly Phe Trp Gly Gly Asn Ser Val Ser Glu Asn Val Gly 
            420                 425                 430         


Pro Lys His Leu Leu Asn Ile Lys Thr Val Ala Glu Arg Arg Glu Asn 
        435                 440                 445             


Met Leu Trp Phe Arg Val Pro His Lys Val Tyr Phe Lys Phe Gly Cys 
    450                 455                 460                 


Leu Gln Phe Ala Leu Lys Asp Leu Lys Asp Leu Lys Lys Lys Arg Ala 
465                 470                 475                 480 


Phe Ile Val Thr Asp Ser Asp Pro Tyr Asn Leu Asn Tyr Val Asp Ser 
                485                 490                 495     


Ile Ile Lys Ile Leu Glu His Leu Asp Ile Asp Phe Lys Val Phe Asn 
            500                 505                 510         


Lys Val Gly Arg Glu Ala Asp Leu Lys Thr Ile Lys Lys Ala Thr Glu 
        515                 520                 525             


Glu Met Ser Ser Phe Met Pro Asp Thr Ile Ile Ala Leu Gly Gly Thr 
    530                 535                 540                 


Pro Glu Met Ser Ser Ala Lys Leu Met Trp Val Leu Tyr Glu His Pro 
545                 550                 555                 560 


Glu Val Lys Phe Glu Asp Leu Ala Ile Lys Phe Met Asp Ile Arg Lys 
                565                 570                 575     


Arg Ile Tyr Thr Phe Pro Lys Leu Gly Lys Lys Ala Met Leu Val Ala 
            580                 585                 590         


Ile Thr Thr Ser Ala Gly Ser Gly Ser Glu Val Thr Pro Phe Ala Leu 
        595                 600                 605             


Val Thr Asp Asn Asn Thr Gly Asn Lys Tyr Met Leu Ala Asp Tyr Glu 
    610                 615                 620                 


Met Thr Pro Asn Met Ala Ile Val Asp Ala Glu Leu Met Met Lys Met 
625                 630                 635                 640 


Pro Lys Gly Leu Thr Ala Tyr Ser Gly Ile Asp Ala Leu Val Asn Ser 
                645                 650                 655     


Ile Glu Ala Tyr Thr Ser Val Tyr Ala Ser Glu Tyr Thr Asn Gly Leu 
            660                 665                 670         


Ala Leu Glu Ala Ile Arg Leu Ile Phe Lys Tyr Leu Pro Glu Ala Tyr 
        675                 680                 685             


Lys Asn Gly Arg Thr Asn Glu Lys Ala Arg Glu Lys Met Ala His Ala 
    690                 695                 700                 


Ser Thr Met Ala Gly Met Ala Ser Ala Asn Ala Phe Leu Gly Leu Cys 
705                 710                 715                 720 


His Ser Met Ala Ile Lys Leu Ser Ser Glu His Asn Ile Pro Ser Gly 
                725                 730                 735     


Ile Ala Asn Ala Leu Leu Ile Glu Glu Val Ile Lys Phe Asn Ala Val 
            740                 745                 750         


Asp Asn Pro Val Lys Gln Ala Pro Cys Pro Gln Tyr Lys Tyr Pro Asn 
        755                 760                 765             


Thr Ile Phe Arg Tyr Ala Arg Ile Ala Asp Tyr Ile Lys Leu Gly Gly 
    770                 775                 780                 


Asn Thr Asp Glu Glu Lys Val Asp Leu Leu Ile Asn Lys Ile His Glu 
785                 790                 795                 800 


Leu Lys Lys Ala Leu Asn Ile Pro Thr Ser Ile Lys Asp Ala Gly Val 
                805                 810                 815     


Leu Glu Glu Asn Phe Tyr Ser Ser Leu Asp Arg Ile Ser Glu Leu Ala 
            820                 825                 830         


Leu Asp Asp Gln Cys Thr Gly Ala Asn Pro Arg Phe Pro Leu Thr Ser 
        835                 840                 845             


Glu Ile Lys Glu Met Tyr Ile Asn Cys Phe Lys Lys Gln Pro 
    850                 855                 860         


<210>  154
<211>  1164
<212>  DNA
<213>  Escherichia coli

<400>  154
atgaacaact ttaatctgca caccccaacc cgcattctgt ttggtaaagg cgcaatcgct     60

ggtttacgcg aacaaattcc tcacgatgct cgcgtattga ttacctacgg cggcggcagc    120

gtgaaaaaaa ccggcgttct cgatcaagtt ctggatgccc tgaaaggcat ggacgtgctg    180

gaatttggcg gtattgagcc aaacccggct tatgaaacgc tgatgaacgc cgtgaaactg    240

gttcgcgaac agaaagtgac tttcctgctg gcggttggcg gcggttctgt actggacggc    300

accaaattta tcgccgcagc ggctaactat ccggaaaata tcgatccgtg gcacattctg    360

caaacgggcg gtaaagagat taaaagcgcc atcccgatgg gctgtgtgct gacgctgcca    420

gcaaccggtt cagaatccaa cgcaggcgcg gtgatctccc gtaaaaccac aggcgacaag    480

caggcgttcc attctgccca tgttcagccg gtatttgccg tgctcgatcc ggtttatacc    540

tacaccctgc cgccgcgtca ggtggctaac ggcgtagtgg acgcctttgt acacaccgtg    600

gaacagtatg ttaccaaacc ggttgatgcc aaaattcagg accgtttcgc agaaggcatt    660

ttgctgacgc taatcgaaga tggtccgaaa gccctgaaag agccagaaaa ctacgatgtg    720

cgcgccaacg tcatgtgggc ggcgactcag gcgctgaacg gtttgattgg cgctggcgta    780

ccgcaggact gggcaacgca tatgctgggc cacgaactga ctgcgatgca cggtctggat    840

cacgcgcaaa cactggctat cgtcctgcct gcactgtgga atgaaaaacg cgataccaag    900

cgcgctaagc tgctgcaata tgctgaacgc gtctggaaca tcactgaagg ttccgatgat    960

gagcgtattg acgccgcgat tgccgcaacc cgcaatttct ttgagcaatt aggcgtgccg   1020

acccacctct ccgactacgg tctggacggc agctccatcc cggctttgct gaaaaaactg   1080

gaagagcacg gcatgaccca actgggcgaa aatcatgaca ttacgttgga tgtcagccgc   1140

cgtatatacg aagccgcccg ctaa                                          1164


<210>  155
<211>  387
<212>  PRT
<213>  Escherichia coli

<400>  155

Met Asn Asn Phe Asn Leu His Thr Pro Thr Arg Ile Leu Phe Gly Lys 
1               5                   10                  15      


Gly Ala Ile Ala Gly Leu Arg Glu Gln Ile Pro His Asp Ala Arg Val 
            20                  25                  30          


Leu Ile Thr Tyr Gly Gly Gly Ser Val Lys Lys Thr Gly Val Leu Asp 
        35                  40                  45              


Gln Val Leu Asp Ala Leu Lys Gly Met Asp Val Leu Glu Phe Gly Gly 
    50                  55                  60                  


Ile Glu Pro Asn Pro Ala Tyr Glu Thr Leu Met Asn Ala Val Lys Leu 
65                  70                  75                  80  


Val Arg Glu Gln Lys Val Thr Phe Leu Leu Ala Val Gly Gly Gly Ser 
                85                  90                  95      


Val Leu Asp Gly Thr Lys Phe Ile Ala Ala Ala Ala Asn Tyr Pro Glu 
            100                 105                 110         


Asn Ile Asp Pro Trp His Ile Leu Gln Thr Gly Gly Lys Glu Ile Lys 
        115                 120                 125             


Ser Ala Ile Pro Met Gly Cys Val Leu Thr Leu Pro Ala Thr Gly Ser 
    130                 135                 140                 


Glu Ser Asn Ala Gly Ala Val Ile Ser Arg Lys Thr Thr Gly Asp Lys 
145                 150                 155                 160 


Gln Ala Phe His Ser Ala His Val Gln Pro Val Phe Ala Val Leu Asp 
                165                 170                 175     


Pro Val Tyr Thr Tyr Thr Leu Pro Pro Arg Gln Val Ala Asn Gly Val 
            180                 185                 190         


Val Asp Ala Phe Val His Thr Val Glu Gln Tyr Val Thr Lys Pro Val 
        195                 200                 205             


Asp Ala Lys Ile Gln Asp Arg Phe Ala Glu Gly Ile Leu Leu Thr Leu 
    210                 215                 220                 


Ile Glu Asp Gly Pro Lys Ala Leu Lys Glu Pro Glu Asn Tyr Asp Val 
225                 230                 235                 240 


Arg Ala Asn Val Met Trp Ala Ala Thr Gln Ala Leu Asn Gly Leu Ile 
                245                 250                 255     


Gly Ala Gly Val Pro Gln Asp Trp Ala Thr His Met Leu Gly His Glu 
            260                 265                 270         


Leu Thr Ala Met His Gly Leu Asp His Ala Gln Thr Leu Ala Ile Val 
        275                 280                 285             


Leu Pro Ala Leu Trp Asn Glu Lys Arg Asp Thr Lys Arg Ala Lys Leu 
    290                 295                 300                 


Leu Gln Tyr Ala Glu Arg Val Trp Asn Ile Thr Glu Gly Ser Asp Asp 
305                 310                 315                 320 


Glu Arg Ile Asp Ala Ala Ile Ala Ala Thr Arg Asn Phe Phe Glu Gln 
                325                 330                 335     


Leu Gly Val Pro Thr His Leu Ser Asp Tyr Gly Leu Asp Gly Ser Ser 
            340                 345                 350         


Ile Pro Ala Leu Leu Lys Lys Leu Glu Glu His Gly Met Thr Gln Leu 
        355                 360                 365             


Gly Glu Asn His Asp Ile Thr Leu Asp Val Ser Arg Arg Ile Tyr Glu 
    370                 375                 380                 


Ala Ala Arg 
385         


<210>  156
<211>  3883
<212>  DNA
<213>  Escherichia coli

<400>  156
ctatattgct gaaggtacag gcgtttccat aactatttgc tcgcgttttt tactcaagaa     60

gaaaatgcca aatagcaaca tcaggcagac aatacccgaa attgcgaaga aaactgtctg    120

gtagcctgcg tggtcaaaga gtatcccagt cggcgttgaa agcagcacaa tcccaagcga    180

actggcaatt tgaaaaccaa tcagaaagat cgtcgacgac aggcgcttat caaagtttgc    240

cacgctgtat ttgaagacgg atatgacaca aagtggaacc tcaatggcat gtaacaactt    300

cactaatgaa ataatccagg ggttaacgaa cagcgcgcag gaaaggatac gcaacgccat    360

aatcacaact ccgataagta atgcattttt tggccctacc cgattcacaa agaaaggaat    420

aatcgccatg cacagcgctt cgagtaccac ctggaatgag ttgagataac catacaggcg    480

cgttcctaca tcgtgtgatt cgaataaacc tgaataaaag acaggaaaaa gttgttgatc    540

aaaaatgtta tagaaagacc acgtccccac aataaatatg acgaaaaccc agaagtttcg    600

atccttgaaa actgcgataa aatcctcttt ttttacccct cccgcatctg ccgctacgca    660

ctggtgatcc ttatctttaa aacgcatgtt gatcatcata aatacagcgc caaatagcga    720

gaccaaccag aagttgatat ggggactgat actaaaaaat atgccggcaa agaacgcgcc    780

aatagcatag ccaaaagatc cccaggcgcg cgctgttcca tattcgaaat gaaaatttcg    840

cgccattttt tcggtgaagc tatcaagcaa accgcatccc gccagatacc ccaagccaaa    900

aaatagcgcc cccagaatta gacctacaga aaaattgctt tgcagtaacg gttcataaac    960

gtaaatcata aacggtccgg tcaagaccag gatgaaactc atacaccaga tgagcggttt   1020

cttcagaccg agtttatcct gaacgatgcc gtagaacatc ataaatagaa tgctggtaaa   1080

ctggttgacc gaataaagtg tacctaattc cgtccctgtc aaccctagat gtcctttcag   1140

ccaaatagcg tataacgacc accacagcga ccaggaaata aaaaagagaa atgagtaact   1200

ggatgcaaaa cgatagtacg catttctgaa tggaatattc agtgccataa ttacctgcct   1260

gtcgttaaaa aattcacgtc ctatttagag ataagagcga cttcgccgtt tacttctcac   1320

tattccagtt cttgtcgaca tggcagcgct gtcattgccc ctttcgccgt tactgcaagc   1380

gctccgcaac gttgagcgag atcgataatt cgtcgcattt ctctctcatc tgtagataat   1440

cccgtagagg acagacctgt gagtaacccg gcaacgaacg catctcccgc ccccgtgcta   1500

tcgacacaat tcacagacat tccagcaaaa tggtgaactt gtcctcgata acagaccacc   1560

accccttctg cacctttagt caccaacagc atggcgatct catactcttt tgccagggcg   1620

catatatcct gatcgttctg tgtttttcca ctgataagtc gccattcttc ttccgagagc   1680

ttgacgacat ccgccagttg tagcgcctgc cgcaaacaca agcggagcaa atgctcgtct   1740

tgccatagat cttcacgaat attaggatcg aagctgacaa aacctccggc atgccggatc   1800

gccgtcatcg cagtaaatgc gctggtacgc gaaggctcgg cagacaacgc aattgaacag   1860

agatgtaacc attcgccatg tcgccagcag ggcaagtctg tcgtctctaa aaaaagatcg   1920

gcactggggc ggaccataaa cgtaaatgaa cgttcccctt gatcgttcag atcgacaagc   1980

accgtggatg tccggtgcca ttcatcttgc ttcagatacg tgatatcgac tccctcagtt   2040

agcagcgttc tttgcattaa cgcaccaaaa ggatcatccc ccacccgacc tataaaccca   2100

cttgttccgc ctaatctggc gattcccacc gcaacgttag ctggcgcgcc gccaggacaa   2160

ggcagtaggc gcccgtctga ttctggcaag agatctacga ccgcatcccc taaaacccat   2220

actttggctg acattttttt cccttaaatt catctgagtt acgcatagtg ataaacctct   2280

ttttcgcaaa atcgtcatgg atttactaaa acatgcatat tcgatcacaa aacgtcatag   2340

ttaacgttaa catttgtgat attcatcgca tttatgaaag taagggactt tatttttata   2400

aaagttaacg ttaacaattc accaaatttg cttaaccagg atgattaaaa tgacgcaatc   2460

tcgattgcat gcggcgcaaa acgccctagc aaaacttcat gagcaccggg gtaacacttt   2520

ctatccccat tttcacctcg cgcctcctgc cgggtggatg aacgatccaa acggcctgat   2580

ctggtttaac gatcgttatc acgcgtttta tcaacatcat ccgatgagcg aacactgggg   2640

gccaatgcac tggggacatg ccaccagcga cgatatgatc cactggcagc atgagcctat   2700

tgcgctagcg ccaggagacg ataatgacaa agacgggtgt ttttcaggta gtgctgtcga   2760

tgacaatggt gtcctctcac ttatctacac cggacacgtc tggctcgatg gtgcaggtaa   2820

tgacgatgca attcgcgaag tacaatgtct ggctaccagt cgggatggta ttcatttcga   2880

gaaacagggt gtgatcctca ctccaccaga aggaatcatg cacttccgcg atcctaaagt   2940

gtggcgtgaa gccgacacat ggtggatggt agtcggggcg aaagatccag gcaacacggg   3000

gcagatcctg ctttatcgcg gcagttcgtt gcgtgaatgg accttcgatc gcgtactggc   3060

ccacgctgat gcgggtgaaa gctatatgtg ggaatgtccg gactttttca gccttggcga   3120

tcagcattat ctgatgtttt ccccgcaggg aatgaatgcc gagggataca gttaccgaaa   3180

tcgctttcaa agtggcgtaa tacccggaat gtggtcgcca ggacgacttt ttgcacaatc   3240

cgggcatttt actgaacttg ataacgggca tgacttttat gcaccacaaa gctttttagc   3300

gaaggatggt cggcgtattg ttatcggctg gatggatatg tgggaatcgc caatgccctc   3360

aaaacgtgaa ggatgggcag gctgcatgac gctggcgcgc gagctatcag agagcaatgg   3420

caaacttcta caacgcccgg tacacgaagc tgagtcgtta cgccagcagc atcaatctgt   3480

ctctccccgc acaatcagca ataaatatgt tttgcaggaa aacgcgcaag cagttgagat   3540

tcagttgcag tgggcgctga agaacagtga tgccgaacat tacggattac agctcggcac   3600

tggaatgcgg ctgtatattg ataaccaatc tgagcgactt gttttgtggc ggtattaccc   3660

acacgagaat ttagacggct accgtagtat tcccctcccg cagcgtgaca cgctcgccct   3720

aaggatattt atcgatacat catccgtgga agtatttatt aacgacgggg aagcggtgat   3780

gagtagtcga atctatccgc agccagaaga acgggaactg tcgctttatg cctcccacgg   3840

agtggctgtg ctgcaacatg gagcactctg gctactgggt taa                     3883


<210>  157
<211>  477
<212>  PRT
<213>  Escherichia coli

<400>  157

Met Thr Gln Ser Arg Leu His Ala Ala Gln Asn Ala Leu Ala Lys Leu 
1               5                   10                  15      


His Glu His Arg Gly Asn Thr Phe Tyr Pro His Phe His Leu Ala Pro 
            20                  25                  30          


Pro Ala Gly Trp Met Asn Asp Pro Asn Gly Leu Ile Trp Phe Asn Asp 
        35                  40                  45              


Arg Tyr His Ala Phe Tyr Gln His His Pro Met Ser Glu His Trp Gly 
    50                  55                  60                  


Pro Met His Trp Gly His Ala Thr Ser Asp Asp Met Ile His Trp Gln 
65                  70                  75                  80  


His Glu Pro Ile Ala Leu Ala Pro Gly Asp Asp Asn Asp Lys Asp Gly 
                85                  90                  95      


Cys Phe Ser Gly Ser Ala Val Asp Asp Asn Gly Val Leu Ser Leu Ile 
            100                 105                 110         


Tyr Thr Gly His Val Trp Leu Asp Gly Ala Gly Asn Asp Asp Ala Ile 
        115                 120                 125             


Arg Glu Val Gln Cys Leu Ala Thr Ser Arg Asp Gly Ile His Phe Glu 
    130                 135                 140                 


Lys Gln Gly Val Ile Leu Thr Pro Pro Glu Gly Ile Met His Phe Arg 
145                 150                 155                 160 


Asp Pro Lys Val Trp Arg Glu Ala Asp Thr Trp Trp Met Val Val Gly 
                165                 170                 175     


Ala Lys Asp Pro Gly Asn Thr Gly Gln Ile Leu Leu Tyr Arg Gly Ser 
            180                 185                 190         


Ser Leu Arg Glu Trp Thr Phe Asp Arg Val Leu Ala His Ala Asp Ala 
        195                 200                 205             


Gly Glu Ser Tyr Met Trp Glu Cys Pro Asp Phe Phe Ser Leu Gly Asp 
    210                 215                 220                 


Gln His Tyr Leu Met Phe Ser Pro Gln Gly Met Asn Ala Glu Gly Tyr 
225                 230                 235                 240 


Ser Tyr Arg Asn Arg Phe Gln Ser Gly Val Ile Pro Gly Met Trp Ser 
                245                 250                 255     


Pro Gly Arg Leu Phe Ala Gln Ser Gly His Phe Thr Glu Leu Asp Asn 
            260                 265                 270         


Gly His Asp Phe Tyr Ala Pro Gln Ser Phe Leu Ala Lys Asp Gly Arg 
        275                 280                 285             


Arg Ile Val Ile Gly Trp Met Asp Met Trp Glu Ser Pro Met Pro Ser 
    290                 295                 300                 


Lys Arg Glu Gly Trp Ala Gly Cys Met Thr Leu Ala Arg Glu Leu Ser 
305                 310                 315                 320 


Glu Ser Asn Gly Lys Leu Leu Gln Arg Pro Val His Glu Ala Glu Ser 
                325                 330                 335     


Leu Arg Gln Gln His Gln Ser Val Ser Pro Arg Thr Ile Ser Asn Lys 
            340                 345                 350         


Tyr Val Leu Gln Glu Asn Ala Gln Ala Val Glu Ile Gln Leu Gln Trp 
        355                 360                 365             


Ala Leu Lys Asn Ser Asp Ala Glu His Tyr Gly Leu Gln Leu Gly Thr 
    370                 375                 380                 


Gly Met Arg Leu Tyr Ile Asp Asn Gln Ser Glu Arg Leu Val Leu Trp 
385                 390                 395                 400 


Arg Tyr Tyr Pro His Glu Asn Leu Asp Gly Tyr Arg Ser Ile Pro Leu 
                405                 410                 415     


Pro Gln Arg Asp Thr Leu Ala Leu Arg Ile Phe Ile Asp Thr Ser Ser 
            420                 425                 430         


Val Glu Val Phe Ile Asn Asp Gly Glu Ala Val Met Ser Ser Arg Ile 
        435                 440                 445             


Tyr Pro Gln Pro Glu Glu Arg Glu Leu Ser Leu Tyr Ala Ser His Gly 
    450                 455                 460                 


Val Ala Val Leu Gln His Gly Ala Leu Trp Leu Leu Gly 
465                 470                 475         


<210>  158
<211>  304
<212>  PRT
<213>  Escherichia coli

<400>  158

Met Ser Ala Lys Val Trp Val Leu Gly Asp Ala Val Val Asp Leu Leu 
1               5                   10                  15      


Pro Glu Ser Asp Gly Arg Leu Leu Pro Cys Pro Gly Gly Ala Pro Ala 
            20                  25                  30          


Asn Val Ala Val Gly Ile Ala Arg Leu Gly Gly Thr Ser Gly Phe Ile 
        35                  40                  45              


Gly Arg Val Gly Asp Asp Pro Phe Gly Ala Leu Met Gln Arg Thr Leu 
    50                  55                  60                  


Leu Thr Glu Gly Val Asp Ile Thr Tyr Leu Lys Gln Asp Glu Trp His 
65                  70                  75                  80  


Arg Thr Ser Thr Val Leu Val Asp Leu Asn Asp Gln Gly Glu Arg Ser 
                85                  90                  95      


Phe Thr Phe Met Val Arg Pro Ser Ala Asp Leu Phe Leu Glu Thr Thr 
            100                 105                 110         


Asp Leu Pro Cys Trp Arg His Gly Glu Trp Leu His Leu Cys Ser Ile 
        115                 120                 125             


Ala Leu Ser Ala Glu Pro Ser Arg Thr Ser Ala Phe Thr Ala Met Thr 
    130                 135                 140                 


Ala Ile Arg His Ala Gly Gly Phe Val Ser Phe Asp Pro Asn Ile Arg 
145                 150                 155                 160 


Glu Asp Leu Trp Gln Asp Glu His Leu Leu Arg Leu Cys Leu Arg Gln 
                165                 170                 175     


Ala Leu Gln Leu Ala Asp Val Val Lys Leu Ser Glu Glu Glu Trp Arg 
            180                 185                 190         


Leu Ile Ser Gly Lys Thr Gln Asn Asp Gln Asp Ile Cys Ala Leu Ala 
        195                 200                 205             


Lys Glu Tyr Glu Ile Ala Met Leu Leu Val Thr Lys Gly Ala Glu Gly 
    210                 215                 220                 


Val Val Val Cys Tyr Arg Gly Gln Val His His Phe Ala Gly Met Ser 
225                 230                 235                 240 


Val Asn Cys Val Asp Ser Thr Gly Ala Gly Asp Ala Phe Val Ala Gly 
                245                 250                 255     


Leu Leu Thr Gly Leu Ser Ser Thr Gly Leu Ser Thr Asp Glu Arg Glu 
            260                 265                 270         


Met Arg Arg Ile Ile Asp Leu Ala Gln Arg Cys Gly Ala Leu Ala Val 
        275                 280                 285             


Thr Ala Lys Gly Ala Met Thr Ala Leu Pro Cys Arg Gln Glu Leu Glu 
    290                 295                 300                 


<210>  159
<211>  415
<212>  PRT
<213>  Escherichia coli

<400>  159

Met Ala Leu Asn Ile Pro Phe Arg Asn Ala Tyr Tyr Arg Phe Ala Ser 
1               5                   10                  15      


Ser Tyr Ser Phe Leu Phe Phe Ile Ser Trp Ser Leu Trp Trp Ser Leu 
            20                  25                  30          


Tyr Ala Ile Trp Leu Lys Gly His Leu Gly Leu Thr Gly Thr Glu Leu 
        35                  40                  45              


Gly Thr Leu Tyr Ser Val Asn Gln Phe Thr Ser Ile Leu Phe Met Met 
    50                  55                  60                  


Phe Tyr Gly Ile Val Gln Asp Lys Leu Gly Leu Lys Lys Pro Leu Ile 
65                  70                  75                  80  


Trp Cys Met Ser Phe Ile Leu Val Leu Thr Gly Pro Phe Met Ile Tyr 
                85                  90                  95      


Val Tyr Glu Pro Leu Leu Gln Ser Asn Phe Ser Val Gly Leu Ile Leu 
            100                 105                 110         


Gly Ala Leu Phe Phe Gly Leu Gly Tyr Leu Ala Gly Cys Gly Leu Leu 
        115                 120                 125             


Asp Ser Phe Thr Glu Lys Met Ala Arg Asn Phe His Phe Glu Tyr Gly 
    130                 135                 140                 


Thr Ala Arg Ala Trp Gly Ser Phe Gly Tyr Ala Ile Gly Ala Phe Phe 
145                 150                 155                 160 


Ala Gly Ile Phe Phe Ser Ile Ser Pro His Ile Asn Phe Trp Leu Val 
                165                 170                 175     


Ser Leu Phe Gly Ala Val Phe Met Met Ile Asn Met Arg Phe Lys Asp 
            180                 185                 190         


Lys Asp His Gln Cys Val Ala Ala Asp Ala Gly Gly Val Lys Lys Glu 
        195                 200                 205             


Asp Phe Ile Ala Val Phe Lys Asp Arg Asn Phe Trp Val Phe Val Ile 
    210                 215                 220                 


Phe Ile Val Gly Thr Trp Ser Phe Tyr Asn Ile Phe Asp Gln Gln Leu 
225                 230                 235                 240 


Phe Pro Val Phe Tyr Ser Gly Leu Phe Glu Ser His Asp Val Gly Thr 
                245                 250                 255     


Arg Leu Tyr Gly Tyr Leu Asn Ser Phe Gln Val Val Leu Glu Ala Leu 
            260                 265                 270         


Cys Met Ala Ile Ile Pro Phe Phe Val Asn Arg Val Gly Pro Lys Asn 
        275                 280                 285             


Ala Leu Leu Ile Gly Val Val Ile Met Ala Leu Arg Ile Leu Ser Cys 
    290                 295                 300                 


Ala Leu Phe Val Asn Pro Trp Ile Ile Ser Leu Val Lys Leu Leu His 
305                 310                 315                 320 


Ala Ile Glu Val Pro Leu Cys Val Ile Ser Val Phe Lys Tyr Ser Val 
                325                 330                 335     


Ala Asn Phe Asp Lys Arg Leu Ser Ser Thr Ile Phe Leu Ile Gly Phe 
            340                 345                 350         


Gln Ile Ala Ser Ser Leu Gly Ile Val Leu Leu Ser Thr Pro Thr Gly 
        355                 360                 365             


Ile Leu Phe Asp His Ala Gly Tyr Gln Thr Val Phe Phe Ala Ile Ser 
    370                 375                 380                 


Gly Ile Val Cys Leu Met Leu Leu Phe Gly Ile Phe Phe Leu Ser Lys 
385                 390                 395                 400 


Lys Arg Glu Gln Ile Val Met Glu Thr Pro Val Pro Ser Ala Ile 
                405                 410                 415 


<210>  160
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Scr1

<400>  160
cctttctttg tgaatcgg                                                   18


<210>  161
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Scr2

<400>  161
agaaacaggg tgtgatcc                                                   18


<210>  162
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Scr3

<400>  162
agtgatcatc acctgttgcc                                                 20


<210>  163
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Scr4

<400>  163
agcacggcga gagtcgacgg                                                 20


<210>  164
<211>  74
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer OT731

<400>  164
aaagctggag ctccaccgcg gtggcggccg ctctagaagt tttcaaagca gagtttcgtt     60

tgaatatttt acca                                                       74


<210>  165
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer OT732

<400>  165
ttcaatatgc atgcctcaga acgtttacat tgtatcgact gccagaaccc                50


<210>  166
<211>  79
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer OT733

<400>  166
gcagtcgata caatgtaaac gttctgaggc atgcatattg aattttcaaa aattcttact     60

ttttttttgg atggacgca                                                  79


<210>  167
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer OT734

<400>  167
acctgcacct ataacacata ccttttccat ggtagttttt tctccttgac gttaaagtat     60

agaggtatat ta                                                         72


<210>  168
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer OT735

<400>  168
aaaaactacc atggaaaagg tatgtgttat aggtgcaggt actatgggtt caggaattgc     60


<210>  169
<211>  71
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer OT736

<400>  169
gtaaaaaaaa gaaggccgta taggccttat tttgaataat cgtagaaacc ttttcctgat     60

tttcttccaa g                                                          71


<210>  170
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer OT737

<400>  170
acgattattc aaaataaggc ctatacggcc ttcttttttt tactttgttc agaacaactt     60

ctcatttttt tctactcata a                                               81


<210>  171
<211>  73
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer OT738

<400>  171
gaattgggta ccgggccccc cctcgaggtc gaccgatgcc tcataaactt cggtagttat     60

attactctga gat                                                        73


<210>  172
<211>  65
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer OT797

<400>  172
aaagtaagaa tttttgaaaa ttcaatatgc atgcaagaag ttgtaatagc tagtgcagta     60

agaac                                                                 65


<210>  173
<211>  73
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer OT798

<400>  173
gaaaaagatc atgagaaaat cgcagaacgt aaggcgcgcc tcagcacttt tctagcaata     60

ttgctgttcc ttg                                                        73


<210>  174
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer OT806

<400>  174
ctcgaaaata gggcgcgccc ccattaccga catttgggcg c                         41


<210>  175
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer OT807

<400>  175
actgcactag ctattacaac ttcttgcatg cgtgatgatt gattgattga ttgta          55


<210>  176
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer OT808

<400>  176
actgcactag ctattacaac ttcttgcatg cgtgatgatt gattgattga ttgta          55


<210>  177
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer OT809

<400>  177
tttcgaataa acacacataa acaaacaccc catggaaaag gtatgtgtta taggtgcagg     60


<210>  178
<211>  62
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer OT799

<400>  178
taccgggccc cccctcgagg tcgacggcgc gccactggta gagagcgact ttgtatgccc     60

ca                                                                    62


<210>  179
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer OT761

<400>  179
cttggccttc actagcatgc tgaatatgta ttacttggtt atggttatat atgacaaaag     60


<210>  180
<211>  68
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer OT803

<400>  180
ccctcactaa agggaacaaa agctggagct cgatatcggc gcgcccacat gcagtgatgc     60

acgcgcga                                                              68


<210>  181
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer OT804

<400>  181
aaggatgaca ttgtttagtt ccatggttgt aatatgtgtg tttgtttgg                 49


<210>  182
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer OT785

<400>  182
cacacatatt acaaccatgg aactaaacaa tgtcatcctt gaaaaggaag g              51


<210>  183
<211>  63
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer OT786

<400>  183
atcattcatt ggccattcag gccttatcta tttttgaagc cttcaatttt tcttttctct     60

atg                                                                   63


<210>  184
<211>  65
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer OT787

<400>  184
caaaaataga taaggcctga atggccaatg aatgatttga tgatttcttt ttccctccat     60

ttttc                                                                 65


<210>  185
<211>  77
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer PT805

<400>  185
gaattgggta ccgggccccc cctcgaggtc gacttatagt attatatttt ctgatttggt     60

tatagcaagc agcgttt                                                    77


<210>  186
<211>  1269
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized ter

<400>  186
actagtacca taaccaagta atacatattc agcatgctag tgaaggccaa gttcgtcaag     60

ggctttatta gagatgttca tccgtatggg tgtaggagag aagtgttaaa ccagattgac    120

tactgcaaga aagcaattgg ctttaggggc cctaaaaagg ttcttattgt aggtgcttct    180

tcaggcttcg gactagctac tagaatatct gttgcattcg gagggcctga agcccataca    240

atcggtgttt catacgagac tggagctaca gacagaagga taggtacggc tgggtggtac    300

aataatatct tctttaaaga attcgctaag aagaaaggtt tggtggcaaa gaatttcata    360

gaagatgcat tttcgaatga aaccaaggat aaagtgataa agtatataaa ggacgaattt    420

ggtaaaattg atttattcgt atattcttta gctgctccta gaagaaagga ctacaaaacc    480

ggtaatgttt atacctcaag aattaaaaca attctaggtg actttgaagg gcctactatt    540

gacgtagaaa gagatgaaat aactttaaag aaggtatctt ctgctagtat cgaggaaatc    600

gaagaaacac gtaaagtaat gggcggagaa gactggcagg agtggtgtga ggagttatta    660

tacgaagatt gtttttctga taaagctaca accatcgctt attcctatat tggcagtcct    720

agaacttata aaatatatcg tgaaggaacc attgggattg ctaagaagga tttagaagac    780

aaagccaagt tgatcaacga aaagcttaat agagtcatag gaggtagggc atttgtgtct    840

gttaacaaag ctttagtaac caaggcatct gcttatattc caaccttccc tctatacgct    900

gccatattat ataaagtaat gaaagaaaag aacattcacg aaaattgtat tatgcaaatt    960

gagcgtatgt tctcagagaa aatatactcc aacgaaaaga ttcagttcga tgataagggc   1020

cgtcttagaa tggacgattt agaactaaga aaggatgttc aggatgaagt tgacagaatt   1080

tggtctaaca taacaccaga aaacttcaag gagcttagtg actacaaggg gtataagaaa   1140

gagtttatga atctaaatgg ttttgattta gatggagttg attattccaa ggatcttgat   1200

attgaattac ttagaaaact agagccttaa gcggccgcgt taattcaaat taattgatat   1260

agtactagt                                                           1269


<210>  187
<211>  398
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Modified Ter protein

<400>  187

Met Leu Val Lys Ala Lys Phe Val Lys Gly Phe Ile Arg Asp Val His 
1               5                   10                  15      


Pro Tyr Gly Cys Arg Arg Glu Val Leu Asn Gln Ile Asp Tyr Cys Lys 
            20                  25                  30          


Lys Ala Ile Gly Phe Arg Gly Pro Lys Lys Val Leu Ile Val Gly Ala 
        35                  40                  45              


Ser Ser Gly Phe Gly Leu Ala Thr Arg Ile Ser Val Ala Phe Gly Gly 
    50                  55                  60                  


Pro Glu Ala His Thr Ile Gly Val Ser Tyr Glu Thr Gly Ala Thr Asp 
65                  70                  75                  80  


Arg Arg Ile Gly Thr Ala Gly Trp Tyr Asn Asn Ile Phe Phe Lys Glu 
                85                  90                  95      


Phe Ala Lys Lys Lys Gly Leu Val Ala Lys Asn Phe Ile Glu Asp Ala 
            100                 105                 110         


Phe Ser Asn Glu Thr Lys Asp Lys Val Ile Lys Tyr Ile Lys Asp Glu 
        115                 120                 125             


Phe Gly Lys Ile Asp Leu Phe Val Tyr Ser Leu Ala Ala Pro Arg Arg 
    130                 135                 140                 


Lys Asp Tyr Lys Thr Gly Asn Val Tyr Thr Ser Arg Ile Lys Thr Ile 
145                 150                 155                 160 


Leu Gly Asp Phe Glu Gly Pro Thr Ile Asp Val Glu Arg Asp Glu Ile 
                165                 170                 175     


Thr Leu Lys Lys Val Ser Ser Ala Ser Ile Glu Glu Ile Glu Glu Thr 
            180                 185                 190         


Arg Lys Val Met Gly Gly Glu Asp Trp Gln Glu Trp Cys Glu Glu Leu 
        195                 200                 205             


Leu Tyr Glu Asp Cys Phe Ser Asp Lys Ala Thr Thr Ile Ala Tyr Ser 
    210                 215                 220                 


Tyr Ile Gly Ser Pro Arg Thr Tyr Lys Ile Tyr Arg Glu Gly Thr Ile 
225                 230                 235                 240 


Gly Ile Ala Lys Lys Asp Leu Glu Asp Lys Ala Lys Leu Ile Asn Glu 
                245                 250                 255     


Lys Leu Asn Arg Val Ile Gly Gly Arg Ala Phe Val Ser Val Asn Lys 
            260                 265                 270         


Ala Leu Val Thr Lys Ala Ser Ala Tyr Ile Pro Thr Phe Pro Leu Tyr 
        275                 280                 285             


Ala Ala Ile Leu Tyr Lys Val Met Lys Glu Lys Asn Ile His Glu Asn 
    290                 295                 300                 


Cys Ile Met Gln Ile Glu Arg Met Phe Ser Glu Lys Ile Tyr Ser Asn 
305                 310                 315                 320 


Glu Lys Ile Gln Phe Asp Asp Lys Gly Arg Leu Arg Met Asp Asp Leu 
                325                 330                 335     


Glu Leu Arg Lys Asp Val Gln Asp Glu Val Asp Arg Ile Trp Ser Asn 
            340                 345                 350         


Ile Thr Pro Glu Asn Phe Lys Glu Leu Ser Asp Tyr Lys Gly Tyr Lys 
        355                 360                 365             


Lys Glu Phe Met Asn Leu Asn Gly Phe Asp Leu Asp Gly Val Asp Tyr 
    370                 375                 380                 


Ser Lys Asp Leu Asp Ile Glu Leu Leu Arg Lys Leu Glu Pro 
385                 390                 395             


<210>  188
<211>  1484
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized ald

<400>  188
actagttcga ataaacacac ataaacaaac accatggata aagatacctt aatcccaacc     60

accaaagact tgaaagtgaa gactaatggt gaaaacatca acttaaagaa ttacaaagat    120

aactcttcat gttttggagt atttgaaaat gttgagaatg ccatttcttc tgcagtacat    180

gcacaaaaga ttctttccct acactacaca aaggaacaaa gagagaaaat aatcaccgaa    240

ataagaaaag ccgcattaca gaataaagag gtcttagcca caatgatcct ggaggaaacc    300

cacatgggaa ggtatgagga taaaatcttg aaacatgaat tagtggccaa gtatacccca    360

ggcactgaag atctgacaac aacagcatgg tccggcgata atggactaac agtggttgaa    420

atgagtccat acggagttat cggcgctata actccaagca cgaatccaac agaaaccgtt    480

atctgcaatt ctataggtat gatagctgcg gggaatgcag ttgtatttaa tggtcaccca    540

tgcgccaaaa agtgtgtcgc tttcgcagta gaaatgataa acaaagccat aattagctgt    600

ggtggacctg aaaaccttgt cactactata aagaacccaa ctatggaaag tttagacgct    660

attatcaaac atccatccat aaaattgttg tgcggtacgg gtggcccggg tatggtaaaa    720

acccttctta attctggtaa aaaggccatc ggagctggcg cgggtaatcc tccggttatt    780

gtagacgata cagcagatat cgagaaggcc ggcagaagca ttattgaagg ttgttcgttt    840

gacaacaatc ttccttgtat cgcggaaaaa gaagtgttcg tgtttgaaaa cgttgcagat    900

gatctgatct ctaacatgtt gaaaaacaac gccgtcatta tcaatgaaga ccaagtatcc    960

aagctgatag accttgttct tcaaaagaac aatgaaactc aagaatattt cattaataag   1020

aagtgggttg gtaaggacgc taaactgttt ttggatgaaa tagatgtaga gtcaccaagt   1080

aatgtaaagt gtattatttg tgaagtcaac gcaaaccatc cgttcgttat gacggagttg   1140

atgatgccaa ttttgcctat agttagagtg aaggacattg atgaagccat taaatacgcc   1200

aagatagctg agcagaatag aaaacattcc gcctacattt attctaagaa catcgataac   1260

cttaatagat tcgaacgtga aattgataca actatctttg ttaagaatgc aaagtcattt   1320

gcaggtgtcg gttatgaagc tgagggtttc acaaccttta caattgccgg atccacaggt   1380

gaaggaatca cgtcagctag aaactttacc aggcaaagac gttgtgtcct agcaggttag   1440

ggcctgcagg gccgtgaatt tactttaaat cttgcattac tagt                    1484


<210>  189
<211>  468
<212>  PRT
<213>  Artificial

<220>
<223>  Modified Ald

<400>  189

Met Asp Lys Asp Thr Leu Ile Pro Thr Thr Lys Asp Leu Lys Val Lys 
1               5                   10                  15      


Thr Asn Gly Glu Asn Ile Asn Leu Lys Asn Tyr Lys Asp Asn Ser Ser 
            20                  25                  30          


Cys Phe Gly Val Phe Glu Asn Val Glu Asn Ala Ile Ser Ser Ala Val 
        35                  40                  45              


His Ala Gln Lys Ile Leu Ser Leu His Tyr Thr Lys Glu Gln Arg Glu 
    50                  55                  60                  


Lys Ile Ile Thr Glu Ile Arg Lys Ala Ala Leu Gln Asn Lys Glu Val 
65                  70                  75                  80  


Leu Ala Thr Met Ile Leu Glu Glu Thr His Met Gly Arg Tyr Glu Asp 
                85                  90                  95      


Lys Ile Leu Lys His Glu Leu Val Ala Lys Tyr Thr Pro Gly Thr Glu 
            100                 105                 110         


Asp Leu Thr Thr Thr Ala Trp Ser Gly Asp Asn Gly Leu Thr Val Val 
        115                 120                 125             


Glu Met Ser Pro Tyr Gly Val Ile Gly Ala Ile Thr Pro Ser Thr Asn 
    130                 135                 140                 


Pro Thr Glu Thr Val Ile Cys Asn Ser Ile Gly Met Ile Ala Ala Gly 
145                 150                 155                 160 


Asn Ala Val Val Phe Asn Gly His Pro Cys Ala Lys Lys Cys Val Ala 
                165                 170                 175     


Phe Ala Val Glu Met Ile Asn Lys Ala Ile Ile Ser Cys Gly Gly Pro 
            180                 185                 190         


Glu Asn Leu Val Thr Thr Ile Lys Asn Pro Thr Met Glu Ser Leu Asp 
        195                 200                 205             


Ala Ile Ile Lys His Pro Ser Ile Lys Leu Leu Cys Gly Thr Gly Gly 
    210                 215                 220                 


Pro Gly Met Val Lys Thr Leu Leu Asn Ser Gly Lys Lys Ala Ile Gly 
225                 230                 235                 240 


Ala Gly Ala Gly Asn Pro Pro Val Ile Val Asp Asp Thr Ala Asp Ile 
                245                 250                 255     


Glu Lys Ala Gly Arg Ser Ile Ile Glu Gly Cys Ser Phe Asp Asn Asn 
            260                 265                 270         


Leu Pro Cys Ile Ala Glu Lys Glu Val Phe Val Phe Glu Asn Val Ala 
        275                 280                 285             


Asp Asp Leu Ile Ser Asn Met Leu Lys Asn Asn Ala Val Ile Ile Asn 
    290                 295                 300                 


Glu Asp Gln Val Ser Lys Leu Ile Asp Leu Val Leu Gln Lys Asn Asn 
305                 310                 315                 320 


Glu Thr Gln Glu Tyr Phe Ile Asn Lys Lys Trp Val Gly Lys Asp Ala 
                325                 330                 335     


Lys Leu Phe Leu Asp Glu Ile Asp Val Glu Ser Pro Ser Asn Val Lys 
            340                 345                 350         


Cys Ile Ile Cys Glu Val Asn Ala Asn His Pro Phe Val Met Thr Glu 
        355                 360                 365             


Leu Met Met Pro Ile Leu Pro Ile Val Arg Val Lys Asp Ile Asp Glu 
    370                 375                 380                 


Ala Ile Lys Tyr Ala Lys Ile Ala Glu Gln Asn Arg Lys His Ser Ala 
385                 390                 395                 400 


Tyr Ile Tyr Ser Lys Asn Ile Asp Asn Leu Asn Arg Phe Glu Arg Glu 
                405                 410                 415     


Ile Asp Thr Thr Ile Phe Val Lys Asn Ala Lys Ser Phe Ala Gly Val 
            420                 425                 430         


Gly Tyr Glu Ala Glu Gly Phe Thr Thr Phe Thr Ile Ala Gly Ser Thr 
        435                 440                 445             


Gly Glu Gly Ile Thr Ser Ala Arg Asn Phe Thr Arg Gln Arg Arg Cys 
    450                 455                 460                 


Val Leu Ala Gly 
465             


<210>  190
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer PT800

<400>  190
gggaacaaaa gctggagctc caccgcggtg gggcgcgccc tattttcgag gaccttgtca     60

ccttgagcc                                                             69


<210>  191
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer OT758

<400>  191
ttaaggtatc tttatccatg gtgtttgttt atgtgtgttt attcgaaact                50


<210>  192
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer OT754

<400>  192
ttgggtaccg ggccccccct cgaggtcgac tggccattaa tctttcccat at             52


<210>  193
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer OT755

<400>  193
tgtgtcctag caggttaggg cctgcagggc cgtgaattta ctttaaatct tg             52


<210>  194
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer OT760

<400>  194
cgaaaatagg gcgcgccact ggtagagagc gactttgtat gccccaattg                50


<210>  195
<211>  71
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer OT792

<400>  195
cccttgacga acttggcctt cactagcatg ctgaatatgt attacttggt tatggttata     60

tatgacaaaa g                                                          71


<210>  196
<211>  71
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer OT791

<400>  196
cccttgacga acttggcctt cactagcatg ctgaatatgt attacttggt tatggttata     60

tatgacaaaa g                                                          71


<210>  197
<211>  73
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer OT765

<400>  197
ggaacaaaag ctggagctcc accgcggtgg tttaacgtat agacttctaa tatatttctc     60

catacttggt att                                                        73


<210>  198
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer LDHEcoRV F

<400>  198
gacgtcatga ccacccgccg atccctttt                                       29


<210>  199
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer LDH AatlR

<400>  199
gatatccaac accagcgacc gacgtattac                                      30


<210>  200
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Cm F

<400>  200
atttaaatct cgagtagagg atcccaacaa acgaaaattg gataaag                   47


<210>  201
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Cm R

<400>  201
acgcgttatt ataaaagcca gtcattagg                                       29


<210>  202
<211>  62
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer P11 F

<400>  202
tcgagagcgc tatagttgtt gacagaatgg acatactatg atatattgtt gctatagcgc     60

cc                                                                    62


<210>  203
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer P11 R

<400>  203
gggcgctata gcaacaatat atcatagtat gtccattctg tcaacaacta tagcgctc       58


<210>  204
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer PldhL F

<400>  204
gagctcgtcg acaaaccaac attatgacgt gtctgggc                             38


<210>  205
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer PldhL R

<400>  205
ggatcctacc atgtttgtgc aaaataagtg                                      30


<210>  206
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer F-PnisA (EcoRV)

<400>  206
ttcagtgata tcgacatact tgaatgacct agtc                                 34


<210>  207
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer R-PnisA(PmeI BamHI)

<400>  207
ttgattagtt taaactgtag gatcctttga gtgcctcctt ataattta                  48


<210>  208
<211>  2460
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Template DNA

<400>  208
tgatccaaag gagggtgagg aaatggcgat gtttacgacc accgcaaaag ttattcagcc     60

gaaaattcgt ggttttattt gcaccaccac ccacccgatt ggttgcgaaa aacgtgttca    120

ggaagaaatc gcatacgcac gcgcgcaccc gccgaccagc ccgggtccga aacgtgtgct    180

ggttattggc tgcagtacgg gctatggcct gagcacccgt atcaccgcgg cctttggtta    240

tcaggccgca accctgggcg tgtttctggc aggcccgccg accaaaggcc gtccggccgc    300

ggcgggttgg tataatacgg ttgcgttcga aaaagccgcc ctggaagcag gtctgtatgc    360

acgttctctg aatggtgatg cgttcgattc taccacgaaa gcccgcaccg tggaagcaat    420

taaacgtgat ctgggtaccg ttgatctggt ggtgtatagc attgcagcgc cgaaacgtac    480

cgatccggcc accggcgtgc tgcataaagc gtgcctgaaa ccgattggtg caacctacac    540

caatcgtacg gtgaacaccg ataaagcaga agttaccgat gtgagtattg aaccggccag    600

tccggaagaa atcgcagata ccgtgaaagt tatgggtggc gaagattggg aactgtggat    660

tcaggcactg agcgaagccg gcgtgctggc cgaaggcgca aaaaccgttg cgtattctta    720

tattggcccg gaaatgacgt ggccggtgta ttggagtggc accattggcg aagccaaaaa    780

agatgttgaa aaagcggcga aacgcatcac ccagcagtac ggctgtccgg cgtatccggt    840

tgttgccaaa gcgctggtga cccaggccag tagcgccatt ccggtggtgc cgctgtatat    900

ttgcctgctg tatcgtgtta tgaaagaaaa aggcacccat gaaggctgca ttgaacagat    960

ggtgcgtctg ctgacgacga aactgtatcc ggaaaatggt gcgccgatcg tggatgaagc   1020

gggccgtgtg cgtgttgatg attgggaaat ggcagaagat gttcagcagg cagttaaaga   1080

tctgtggagc caggtgagta cggccaatct gaaagatatt agcgattttg caggttatca   1140

gaccgaattt ctgcgtctgt ttggctttgg tattgatggt gtggattacg atcagccggt   1200

tgatgttgaa gcggatctgc cgagcgccgc ccagcagtaa gtcaacaaag gaggggttaa   1260

aatggttgat ttcgaatatt caataccaac tagaattttt ttcggtaaag ataagataaa   1320

tgtacttgga agagagctta aaaaatatgg ttctaaagtg cttatagttt atggtggagg   1380

aagtataaag agaaatggaa tatatgataa agctgtaagt atacttgaaa aaaacagtat   1440

taaattttat gaacttgcag gagtagagcc aaatccaaga gtaactacag ttgaaaaagg   1500

agttaaaata tgtagagaaa atggagttga agtagtacta gctataggtg gaggaagtgc   1560

aatagattgc gcaaaggtta tagcagcagc atgtgaatat gatggaaatc catgggatat   1620

tgtgttagat ggctcaaaaa taaaaagggt gcttcctata gctagtatat taaccattgc   1680

tgcaacagga tcagaaatgg atacgtgggc agtaataaat aatatggata caaacgaaaa   1740

actaattgcg gcacatccag atatggctcc taagttttct atattagatc caacgtatac   1800

gtataccgta cctaccaatc aaacagcagc aggaacagct gatattatga gtcatatatt   1860

tgaggtgtat tttagtaata caaaaacagc atatttgcag gatagaatgg cagaagcgtt   1920

attaagaact tgtattaaat atggaggaat agctcttgag aagccggatg attatgaggc   1980

aagagccaat ctaatgtggg cttcaagtct tgcgataaat ggacttttaa catatggtaa   2040

agacactaat tggagtgtac acttaatgga acatgaatta agtgcttatt acgacataac   2100

acacggcgta gggcttgcaa ttttaacacc taattggatg gagtatattt taaataatga   2160

tacagtgtac aagtttgttg aatatggtgt aaatgtttgg ggaatagaca aagaaaaaaa   2220

tcactatgac atagcacatc aagcaataca aaaaacaaga gattactttg taaatgtact   2280

aggtttacca tctagactga gagatgttgg aattgaagaa gaaaaattgg acataatggc   2340

aaaggaatca gtaaagctta caggaggaac cataggaaac ctaagaccag taaacgcctc   2400

cgaagtccta caaatattca aaaaatctgt gtaaacctac gtttaaactt acgcgtatga   2460


