                         SEQUENCE LISTING

<110>  Nucleonics, Inc.
 
<120>  Microbial Host-Vector Complementation System

<130>  NUCL-028/01WO

<150>  US 60/907,901
<151>  2007-04-20

<160>  3     

<170>  PatentIn version 3.4

<210>  1
<211>  1730
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (103)..(1638)

<400>  1
ggtaccgata tctcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag     60

ggattttggt catgagcaag cattttttgc aaaaaggggt ag atg caa tcg gtt       114
                                               Met Gln Ser Val          
                                               1                        

acg ctc tgt ata atg ccg cgg caa tat tta tta acc act ctg gtc gag      162
Thr Leu Cys Ile Met Pro Arg Gln Tyr Leu Leu Thr Thr Leu Val Glu         
5                   10                  15                  20          

ata ttg ccc atg cta cgt atc gct aaa gaa gct ctg acg ttt gac gac      210
Ile Leu Pro Met Leu Arg Ile Ala Lys Glu Ala Leu Thr Phe Asp Asp         
                25                  30                  35              

gtt ctc ctc gtt cct gct cac tct acc gtt ctg ccg aat act gct gac      258
Val Leu Leu Val Pro Ala His Ser Thr Val Leu Pro Asn Thr Ala Asp         
            40                  45                  50                  

ctc agc acc cag ctg acg aaa act att cgt ctg aat atc cct atg ctt      306
Leu Ser Thr Gln Leu Thr Lys Thr Ile Arg Leu Asn Ile Pro Met Leu         
        55                  60                  65                      

tcc gca gca atg gat acc gta acg gaa gcg cgc ctg gct att gct ctg      354
Ser Ala Ala Met Asp Thr Val Thr Glu Ala Arg Leu Ala Ile Ala Leu         
    70                  75                  80                          

gct cag gaa ggc ggt atc ggc ttt atc cac aaa aac atg tcc att gaa      402
Ala Gln Glu Gly Gly Ile Gly Phe Ile His Lys Asn Met Ser Ile Glu         
85                  90                  95                  100         

cgc cag gca gaa gaa gtt cgc cgt gtg aaa aaa cac gaa tct ggt gtg      450
Arg Gln Ala Glu Glu Val Arg Arg Val Lys Lys His Glu Ser Gly Val         
                105                 110                 115             

gtg act gat ccg cag act gtt ctg cca acc acg acg ctg cgc gaa gtg      498
Val Thr Asp Pro Gln Thr Val Leu Pro Thr Thr Thr Leu Arg Glu Val         
            120                 125                 130                 

aaa gaa ctg acc gag cgt aac ggt ttt gcg ggc tat ccg gtc gtt acc      546
Lys Glu Leu Thr Glu Arg Asn Gly Phe Ala Gly Tyr Pro Val Val Thr         
        135                 140                 145                     

gaa gaa aac gaa ctg gtg ggt att atc acc ggt cgt gac gtg cgt ttt      594
Glu Glu Asn Glu Leu Val Gly Ile Ile Thr Gly Arg Asp Val Arg Phe         
    150                 155                 160                         

gtt acc gac ctg aac cag ccg gtt agc gtt tac atg acg ccg aaa gag      642
Val Thr Asp Leu Asn Gln Pro Val Ser Val Tyr Met Thr Pro Lys Glu         
165                 170                 175                 180         

cgt ctg gtc acc gtg cgt gaa ggt gaa gcc cgt gaa gtg gtg ctg gca      690
Arg Leu Val Thr Val Arg Glu Gly Glu Ala Arg Glu Val Val Leu Ala         
                185                 190                 195             

aaa atg cac gaa aaa cgc gtt gaa aaa gcg ctg gtg gtt gat gac gaa      738
Lys Met His Glu Lys Arg Val Glu Lys Ala Leu Val Val Asp Asp Glu         
            200                 205                 210                 

ttc cac ctg atc ggc atg atc acc gtg aaa gac ttc cag aaa gcg gaa      786
Phe His Leu Ile Gly Met Ile Thr Val Lys Asp Phe Gln Lys Ala Glu         
        215                 220                 225                     

gct aaa ccg aac gcc tgt aaa gac gag caa ggc cgt ctg cgt gtt ggt      834
Ala Lys Pro Asn Ala Cys Lys Asp Glu Gln Gly Arg Leu Arg Val Gly         
    230                 235                 240                         

gca gcg gtt ggc gca ggt gcg ggt aac gaa gag cgt gtt gac gcg ctg      882
Ala Ala Val Gly Ala Gly Ala Gly Asn Glu Glu Arg Val Asp Ala Leu         
245                 250                 255                 260         

gtt gcc gca ggc gtt gac gtt ctg ctg atc gac tcc tcc cac ggt cac      930
Val Ala Ala Gly Val Asp Val Leu Leu Ile Asp Ser Ser His Gly His         
                265                 270                 275             

tca gaa ggt gta ctg caa cgt atc cgt gaa acc cgt gct aaa tat ccg      978
Ser Glu Gly Val Leu Gln Arg Ile Arg Glu Thr Arg Ala Lys Tyr Pro         
            280                 285                 290                 

gat ctg caa att atc ggc ggc aac gtg gca aca gct gca ggt gca cgc     1026
Asp Leu Gln Ile Ile Gly Gly Asn Val Ala Thr Ala Ala Gly Ala Arg         
        295                 300                 305                     

gct ctg gca gaa gct ggt tgc agt gcg gtt aaa gtc ggc att ggc cct     1074
Ala Leu Ala Glu Ala Gly Cys Ser Ala Val Lys Val Gly Ile Gly Pro         
    310                 315                 320                         

ggc tct atc tgt aca act cgt atc gtg act ggc gtc ggt gtt ccg cag     1122
Gly Ser Ile Cys Thr Thr Arg Ile Val Thr Gly Val Gly Val Pro Gln         
325                 330                 335                 340         

att acc gct gtt gct gac gca gta gaa gcc ctg gaa ggc acc ggt att     1170
Ile Thr Ala Val Ala Asp Ala Val Glu Ala Leu Glu Gly Thr Gly Ile         
                345                 350                 355             

ccg gtt atc gct gat ggc ggt att cgc ttc tcc ggc gac atc gcc aaa     1218
Pro Val Ile Ala Asp Gly Gly Ile Arg Phe Ser Gly Asp Ile Ala Lys         
            360                 365                 370                 

gct atc gcc gct ggc gca agc gcg gtg atg gta ggt tcc atg ctg gcg     1266
Ala Ile Ala Ala Gly Ala Ser Ala Val Met Val Gly Ser Met Leu Ala         
        375                 380                 385                     

ggt act gaa gaa tct ccg ggt gaa atc gaa ctc tac cag ggc cgt tct     1314
Gly Thr Glu Glu Ser Pro Gly Glu Ile Glu Leu Tyr Gln Gly Arg Ser         
    390                 395                 400                         

tac aaa tct tac cgt ggt atg ggt tcc ctg ggc gcg atg tcc aaa ggt     1362
Tyr Lys Ser Tyr Arg Gly Met Gly Ser Leu Gly Ala Met Ser Lys Gly         
405                 410                 415                 420         

tcc tct gac cgt tat ttc cag agc gat aac gct gcc gac aaa ctg gtg     1410
Ser Ser Asp Arg Tyr Phe Gln Ser Asp Asn Ala Ala Asp Lys Leu Val         
                425                 430                 435             

ccg gaa ggt atc gaa ggt cgc gta gcc tat aaa ggt cgc ctg aaa gag     1458
Pro Glu Gly Ile Glu Gly Arg Val Ala Tyr Lys Gly Arg Leu Lys Glu         
            440                 445                 450                 

atc att cac cag cag atg ggc ggc ctg cgc tcc tgt atg ggt ctg acc     1506
Ile Ile His Gln Gln Met Gly Gly Leu Arg Ser Cys Met Gly Leu Thr         
        455                 460                 465                     

ggc tgt ggt act atc gac gaa ctg cgt act aaa gcg gag ttt gta cgt     1554
Gly Cys Gly Thr Ile Asp Glu Leu Arg Thr Lys Ala Glu Phe Val Arg         
    470                 475                 480                         

atc agc ggt gcg ggc att cag gaa agc cac gtt cac gac gtg acc att     1602
Ile Ser Gly Ala Gly Ile Gln Glu Ser His Val His Asp Val Thr Ile         
485                 490                 495                 500         

act aaa gag tcc ccg aac tac cgt ctg ggc tcc tga ttctcaattc          1648
Thr Lys Glu Ser Pro Asn Tyr Arg Leu Gly Ser                             
                505                 510                                 

agcccgccta atgagcgggc ttttttttaa aaaacctccc acacctcccc ctgaacctga   1708

aacataaaat gaatgcggcc gc                                            1730


<210>  2
<211>  511
<212>  PRT
<213>  Escherichia coli

<400>  2

Met Gln Ser Val Thr Leu Cys Ile Met Pro Arg Gln Tyr Leu Leu Thr 
1               5                   10                  15      


Thr Leu Val Glu Ile Leu Pro Met Leu Arg Ile Ala Lys Glu Ala Leu 
            20                  25                  30          


Thr Phe Asp Asp Val Leu Leu Val Pro Ala His Ser Thr Val Leu Pro 
        35                  40                  45              


Asn Thr Ala Asp Leu Ser Thr Gln Leu Thr Lys Thr Ile Arg Leu Asn 
    50                  55                  60                  


Ile Pro Met Leu Ser Ala Ala Met Asp Thr Val Thr Glu Ala Arg Leu 
65                  70                  75                  80  


Ala Ile Ala Leu Ala Gln Glu Gly Gly Ile Gly Phe Ile His Lys Asn 
                85                  90                  95      


Met Ser Ile Glu Arg Gln Ala Glu Glu Val Arg Arg Val Lys Lys His 
            100                 105                 110         


Glu Ser Gly Val Val Thr Asp Pro Gln Thr Val Leu Pro Thr Thr Thr 
        115                 120                 125             


Leu Arg Glu Val Lys Glu Leu Thr Glu Arg Asn Gly Phe Ala Gly Tyr 
    130                 135                 140                 


Pro Val Val Thr Glu Glu Asn Glu Leu Val Gly Ile Ile Thr Gly Arg 
145                 150                 155                 160 


Asp Val Arg Phe Val Thr Asp Leu Asn Gln Pro Val Ser Val Tyr Met 
                165                 170                 175     


Thr Pro Lys Glu Arg Leu Val Thr Val Arg Glu Gly Glu Ala Arg Glu 
            180                 185                 190         


Val Val Leu Ala Lys Met His Glu Lys Arg Val Glu Lys Ala Leu Val 
        195                 200                 205             


Val Asp Asp Glu Phe His Leu Ile Gly Met Ile Thr Val Lys Asp Phe 
    210                 215                 220                 


Gln Lys Ala Glu Ala Lys Pro Asn Ala Cys Lys Asp Glu Gln Gly Arg 
225                 230                 235                 240 


Leu Arg Val Gly Ala Ala Val Gly Ala Gly Ala Gly Asn Glu Glu Arg 
                245                 250                 255     


Val Asp Ala Leu Val Ala Ala Gly Val Asp Val Leu Leu Ile Asp Ser 
            260                 265                 270         


Ser His Gly His Ser Glu Gly Val Leu Gln Arg Ile Arg Glu Thr Arg 
        275                 280                 285             


Ala Lys Tyr Pro Asp Leu Gln Ile Ile Gly Gly Asn Val Ala Thr Ala 
    290                 295                 300                 


Ala Gly Ala Arg Ala Leu Ala Glu Ala Gly Cys Ser Ala Val Lys Val 
305                 310                 315                 320 


Gly Ile Gly Pro Gly Ser Ile Cys Thr Thr Arg Ile Val Thr Gly Val 
                325                 330                 335     


Gly Val Pro Gln Ile Thr Ala Val Ala Asp Ala Val Glu Ala Leu Glu 
            340                 345                 350         


Gly Thr Gly Ile Pro Val Ile Ala Asp Gly Gly Ile Arg Phe Ser Gly 
        355                 360                 365             


Asp Ile Ala Lys Ala Ile Ala Ala Gly Ala Ser Ala Val Met Val Gly 
    370                 375                 380                 


Ser Met Leu Ala Gly Thr Glu Glu Ser Pro Gly Glu Ile Glu Leu Tyr 
385                 390                 395                 400 


Gln Gly Arg Ser Tyr Lys Ser Tyr Arg Gly Met Gly Ser Leu Gly Ala 
                405                 410                 415     


Met Ser Lys Gly Ser Ser Asp Arg Tyr Phe Gln Ser Asp Asn Ala Ala 
            420                 425                 430         


Asp Lys Leu Val Pro Glu Gly Ile Glu Gly Arg Val Ala Tyr Lys Gly 
        435                 440                 445             


Arg Leu Lys Glu Ile Ile His Gln Gln Met Gly Gly Leu Arg Ser Cys 
    450                 455                 460                 


Met Gly Leu Thr Gly Cys Gly Thr Ile Asp Glu Leu Arg Thr Lys Ala 
465                 470                 475                 480 


Glu Phe Val Arg Ile Ser Gly Ala Gly Ile Gln Glu Ser His Val His 
                485                 490                 495     


Asp Val Thr Ile Thr Lys Glu Ser Pro Asn Tyr Arg Leu Gly Ser 
            500                 505                 510     


<210>  3
<211>  1467
<212>  DNA
<213>  Escherichia coli

<400>  3
atgctacgta tcgctaaaga agctctgacg tttgacgacg ttctcctcgt tcctgctcac     60

tctaccgttc tgccgaatac tgctgacctc agcacccagc tgacgaaaac tattcgtctg    120

aatatcccta tgctttccgc agcaatggat accgtaacgg aagcgcgcct ggctattgct    180

ctggctcagg aaggcggtat cggctttatc cacaaaaaca tgtccattga acgccaggca    240

gaagaagttc gccgtgtgaa aaaacacgaa tctggtgtgg tgactgatcc gcagactgtt    300

ctgccaacca cgacgctgcg cgaagtgaaa gaactgaccg agcgtaacgg ttttgcgggc    360

tatccggtcg ttaccgaaga aaacgaactg gtgggtatta tcaccggtcg tgacgtgcgt    420

tttgttaccg acctgaacca gccggttagc gtttacatga cgccgaaaga gcgtctggtc    480

accgtgcgtg aaggtgaagc ccgtgaagtg gtgctggcaa aaatgcacga aaaacgcgtt    540

gaaaaagcgc tggtggttga tgacgaattc cacctgatcg gcatgatcac cgtgaaagac    600

ttccagaaag cggaacgtaa accgaacgcc tgtaaagacg agcaaggccg tctgcgtgtt    660

ggtgcagcgg ttggcgcagg tgcgggtaac gaagagcgtg ttgacgcgct ggttgccgca    720

ggcgttgacg ttctgctgat cgactcctcc cacggtcact cagaaggtgt actgcaacgt    780

atccgtgaaa cccgtgctaa atatccggat ctgcaaatta tcggcggcaa cgtggcaaca    840

gctgcaggtg cacgcgctct ggcagaagct ggttgcagtg cggttaaagt cggcattggc    900

cctggctcta tctgtacaac tcgtatcgtg actggcgtcg gtgttccgca gattaccgct    960

gttgctgacg cagtagaagc cctggaaggc accggtattc cggttatcgc tgatggcggt   1020

attcgcttct ccggcgacat cgccaaagct atcgccgctg gcgcaagcgc ggtgatggta   1080

ggttccatgc tggcgggtac tgaagaatct ccgggtgaaa tcgaactcta ccagggccgt   1140

tcttacaaat cttaccgtgg tatgggttcc ctgggcgcga tgtccaaagg ttcctctgac   1200

cgttatttcc agagcgataa cgctgccgac aaactggtgc cggaaggtat cgaaggtcgc   1260

gtagcctata aaggtcgcct gaaagagatc attcaccagc agatgggcgg cctgcgctcc   1320

tgtatgggtc tgaccggctg tggtactatc gacgaactgc gtactaaagc ggagtttgta   1380

cgtatcagcg gtgcgggcat tcaggaaagc cacgttcacg acgtgaccat tactaaagag   1440

tccccgaact accgtctggg ctcctga                                       1467


