                         SEQUENCE LISTING

<110>  Beall, Melissa J.
       Ione, Phyllis
       Chandrashekar, Ramaswamy
       Liu, Jiayou
 
<120>  Detection of Anaplasma playts

<130>  06-786

<160>  19    

<170>  PatentIn version 3.3

<210>  1
<211>  29
<212>  DNA
<213>  Artificial

<220>
<223>  Degenerate primer to amplify Anaplasma platys p44 polynucleotide

<400>  1
tatttttatg ttggtytrga ytatwshcc                                       29


<210>  2
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  Degenerate primer to amplify Anaplasma playts polynucleotide


<220>
<221>  misc_feature
<222>  (19)..(19)
<223>  n is a, c, g, or t

<400>  2
gctcagcaga tcgtarcang crttyawcat                                      30


<210>  3
<211>  265
<212>  PRT
<213>  Anaplasma playts


<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (257)..(257)
<223>  Xaa can be any naturally occurring amino acid

<400>  3

Tyr Phe Tyr Val Gly Leu Asp Tyr Xaa Pro Ala Phe Ser Lys Ile Asn 
1               5                   10                  15      


Gly Phe Glu Ile Arg Glu Ser Thr Gly Glu Thr Ala Ala Val Tyr Pro 
            20                  25                  30          


Tyr Met Lys Asp Gly Thr Arg Val Glu Trp Lys Ala Glu Lys Phe Asp 
        35                  40                  45              


Trp Asn Thr Pro Asp Pro Arg Ile Lys Phe Lys Asn Asn Pro Ile Val 
    50                  55                  60                  


Ala Leu Glu Gly Ser Val Gly Tyr Ser Ile Gly Val Ala Arg Val Glu 
65                  70                  75                  80  


Leu Glu Ile Gly Tyr Glu Gln Phe Lys Thr Lys Gly Ile Arg Asp Thr 
                85                  90                  95      


Gly Ser Lys Glu Glu Glu Ala Asp Ala Val Tyr Leu Leu Ala Lys Lys 
            100                 105                 110         


Leu Pro His Thr Leu Val Ser Asp Gln Ser Asp Lys Phe Leu Glu Glu 
        115                 120                 125             


Leu Lys Asn Thr Lys Ala Ala Glu Ile Val Lys Phe Ala Glu Ala Val 
    130                 135                 140                 


Gly Thr Ser Ala Lys Asp Ile Asp Lys Lys Val Cys Lys Lys His Thr 
145                 150                 155                 160 


Asn Asn Ala Ala Asn Ser Trp Lys Cys Glu Gln Pro Gly Ser Gly Thr 
                165                 170                 175     


Glu Thr Ser Ala Lys Ala Phe Ser Glu Ile Phe Thr Lys Ala Gly Val 
            180                 185                 190         


Asn Thr Asp Gly Lys Gly Lys Ala Trp Pro Asn Gly His Thr Asp Ser 
        195                 200                 205             


Ala Ala Lys Ala Glu Asp Leu Ser Thr Ala Leu Asn Arg Glu Leu Thr 
    210                 215                 220                 


Ser Ala Glu Lys Asn Lys Val Ala Gly Leu Leu Thr Arg Thr Ile Ser 
225                 230                 235                 240 


Gly Gly Glu Val Val Glu Ile Arg Ala Val Ser Thr Thr Ser Val Met 
                245                 250                 255     


Xaa Asn Ala Cys Tyr Asp Leu Leu Ser 
            260                 265 


<210>  4
<211>  259
<212>  PRT
<213>  Anaplasma playts

<400>  4

Tyr Phe Tyr Val Gly Leu Asp Tyr Cys Pro Ala Phe Ser Lys Ile Asn 
1               5                   10                  15      


Gly Phe Glu Ile Arg Glu Ser Thr Gly Glu Thr Ala Ala Val Tyr Pro 
            20                  25                  30          


Tyr Met Lys Asp Gly Thr Arg Val Glu Trp Lys Ala Glu Lys Phe Asp 
        35                  40                  45              


Trp Asn Thr Pro Asp Pro Arg Ile Lys Phe Lys Asn Asn Pro Ile Val 
    50                  55                  60                  


Ala Leu Glu Gly Ser Val Gly Tyr Ser Ile Gly Val Ala Arg Val Glu 
65                  70                  75                  80  


Leu Glu Ile Gly Tyr Glu Gln Phe Lys Thr Lys Gly Ile Arg Asp Thr 
                85                  90                  95      


Gly Ser Lys Glu Glu Glu Ala Asp Ala Val Tyr Leu Leu Ala Lys Lys 
            100                 105                 110         


Leu Pro His Thr Leu Val Ser Asp Gln Ser Asp Lys Phe Leu Glu Glu 
        115                 120                 125             


Leu Lys Asn Thr Lys Ala Ala Glu Ile Val Lys Phe Ala Glu Ala Val 
    130                 135                 140                 


Gly Thr Ser Ala Lys Asp Ile Asp Gly Lys Val Cys Lys Lys His Asn 
145                 150                 155                 160 


Gly Asn Ala Ala Gly Ser Trp Gln Cys Thr Gln Thr Gly Ser Glu Thr 
                165                 170                 175     


Ser Gly Lys Thr Leu Ser Glu Ile Phe Thr Lys Ala Gly Val Asp Ala 
            180                 185                 190         


Asn Gly Lys Ala Trp Pro Asn Gly Ser Asp Ala Ala Lys Ala Glu Asp 
        195                 200                 205             


Leu Ser Thr Ala Leu Asn Arg Glu Leu Thr Ser Ala Glu Lys Asn Lys 
    210                 215                 220                 


Val Ala Gly Leu Leu Thr Arg Thr Ile Ser Gly Gly Glu Val Val Glu 
225                 230                 235                 240 


Ile Arg Ala Val Ser Thr Thr Ser Val Met Ile Asn Ala Cys Tyr Asp 
                245                 250                 255     


Leu Leu Ser 
            


<210>  5
<211>  263
<212>  PRT
<213>  Anaplasma playts

<400>  5

Tyr Phe Tyr Val Gly Leu Asp Tyr Ser Pro Ala Phe Ser Lys Ile Asn 
1               5                   10                  15      


Gly Phe Glu Ile Arg Glu Ser Thr Gly Glu Thr Ala Ala Val Tyr Pro 
            20                  25                  30          


Tyr Met Lys Asp Gly Thr Arg Val Glu Trp Lys Ala Glu Lys Phe Asp 
        35                  40                  45              


Trp Asn Thr Pro Asp Pro Arg Ile Lys Phe Lys Asn Asn Pro Ile Val 
    50                  55                  60                  


Ala Leu Glu Gly Ser Val Gly Tyr Ser Ile Gly Val Ala Arg Val Glu 
65                  70                  75                  80  


Leu Glu Ile Gly Tyr Glu Gln Phe Lys Thr Lys Gly Ile Arg Asp Thr 
                85                  90                  95      


Gly Ser Lys Glu Glu Glu Ala Asp Ala Val Tyr Leu Leu Ala Lys Lys 
            100                 105                 110         


Leu Pro His Thr Leu Val Ser Asp Gln Ser Asp Lys Phe Leu Glu Glu 
        115                 120                 125             


Leu Lys Asn Thr Lys Ala Ala Glu Ile Val Lys Phe Ala Glu Ala Val 
    130                 135                 140                 


Gly Thr Ser Ala Lys Asp Ile Asp Gly Lys Val Cys Lys Lys Asn Thr 
145                 150                 155                 160 


Asn Asn Ala Ala Asp Ser Trp Lys Cys Glu Gln Thr Gly Ser Gly Ser 
                165                 170                 175     


Asp Gly Lys Glu Phe Ser Lys Leu Phe Thr Lys Ala Gly Val Asp Ala 
            180                 185                 190         


Asn Glu Lys Gly Lys Ala Trp Pro Asn Gly His Thr Asp Ser Ala Ala 
        195                 200                 205             


Lys Ala Glu Asp Leu Ser Thr Ala Leu Asn Arg Glu Leu Thr Ser Ala 
    210                 215                 220                 


Glu Lys Asn Lys Val Ala Gly Leu Leu Thr Arg Thr Ile Ser Gly Gly 
225                 230                 235                 240 


Glu Val Val Glu Ile Arg Ala Val Ser Thr Thr Ser Val Met Leu Asn 
                245                 250                 255     


Ala Cys Tyr Asp Leu Leu Ser 
            260             


<210>  6
<211>  22
<212>  DNA
<213>  Anaplasma playts

<400>  6
ccggcgttta gtaagataaa tg                                              22


<210>  7
<211>  21
<212>  DNA
<213>  Anaplasma playts

<400>  7
gcaaatttaa cgatctccgc c                                               21


<210>  8
<211>  26
<212>  DNA
<213>  Anaplasma playts

<400>  8
acagtatcgg ggtagcgaga gtagaa                                          26


<210>  9
<211>  27
<212>  DNA
<213>  Anaplasma playts

<400>  9
ggagatcggc tatgaacagt tcaagac                                         27


<210>  10
<211>  22
<212>  PRT
<213>  Anaplasma playts

<400>  10

Lys Asp Gly Thr Arg Val Glu Trp Lys Ala Glu Lys Phe Asp Trp Asn 
1               5                   10                  15      


Thr Pro Asp Pro Arg Ile 
            20          


<210>  11
<211>  26
<212>  PRT
<213>  Anaplasma playts

<400>  11

Lys Asp Gly Thr Arg Val Glu Trp Lys Ala Glu Lys Phe Asp Trp Asn 
1               5                   10                  15      


Thr Pro Asp Pro Arg Ile Lys Phe Lys Asn 
            20                  25      


<210>  12
<211>  265
<212>  PRT
<213>  Anaplasma playts


<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  The X at position 9 can be S or C.

<220>
<221>  MISC_FEATURE
<222>  (153)..(153)
<223>  The X at position 153 can be G or K.

<220>
<221>  MISC_FEATURE
<222>  (159)..(159)
<223>  The X at position 159 can be N or H.

<220>
<221>  MISC_FEATURE
<222>  (160)..(160)
<223>  The X at position 160 can be T or N.

<220>
<221>  MISC_FEATURE
<222>  (161)..(161)
<223>  The X at position 161 can be G or N.

<220>
<221>  MISC_FEATURE
<222>  (165)..(165)
<223>  The X at position 165 can be G or N or D.

<220>
<221>  MISC_FEATURE
<222>  (168)..(168)
<223>  The X at position 168 can be K or Q.

<220>
<221>  MISC_FEATURE
<222>  (170)..(170)
<223>  The X at position 170 can be E or T.

<220>
<221>  MISC_FEATURE
<222>  (172)..(172)
<223>  The X at position 172 can be T or P.

<220>
<221>  MISC_FEATURE
<222>  (175)..(175)
<223>  The X at position 175 can be G or E.

<220>
<221>  MISC_FEATURE
<222>  (176)..(176)
<223>  The X at position 176 can be S or T.

<220>
<221>  MISC_FEATURE
<222>  (177)..(177)
<223>  The X at position 177 can be D or E or S.

<220>
<221>  MISC_FEATURE
<222>  (178)..(178)
<223>  The X at position 178 can be T or absent.

<220>
<221>  MISC_FEATURE
<222>  (179)..(179)
<223>  The X at position 179 can be S or absent.

<220>
<221>  MISC_FEATURE
<222>  (180)..(180)
<223>  The X at position 180 can be G or A.

<220>
<221>  MISC_FEATURE
<222>  (182)..(182)
<223>  The X at position 182 can be E or A or T.

<220>
<221>  MISC_FEATURE
<222>  (183)..(183)
<223>  The X at position 183 can be F or L.

<220>
<221>  MISC_FEATURE
<222>  (185)..(185)
<223>  The X at position 185 can be K or E.

<220>
<221>  MISC_FEATURE
<222>  (186)..(186)
<223>  The X at position 186 can be L or I.

<220>
<221>  MISC_FEATURE
<222>  (193)..(193)
<223>  The X at position 193 can be D or N.

<220>
<221>  MISC_FEATURE
<222>  (194)..(194)
<223>  The X at position 194 can be A or T.

<220>
<221>  MISC_FEATURE
<222>  (195)..(195)
<223>  The X at position 195 can be N or D.

<220>
<221>  MISC_FEATURE
<222>  (196)..(196)
<223>  The X at position 196 can be E or G or absent.

<220>
<221>  MISC_FEATURE
<222>  (197)..(197)
<223>  The X at position 197 can be K or absent.

<220>
<221>  MISC_FEATURE
<222>  (205)..(205)
<223>  The X at position 205 can be H or S.

<220>
<221>  MISC_FEATURE
<222>  (206)..(206)
<223>  The X at position 206 can be T or absent.

<220>
<221>  MISC_FEATURE
<222>  (207)..(207)
<223>  The X at position 207 can be D or absent.

<220>
<221>  MISC_FEATURE
<222>  (208)..(208)
<223>  The X at position 208 can be D or S.

<220>
<221>  MISC_FEATURE
<222>  (257)..(257)
<223>  The X at position 257 can be L or I.

<400>  12

Tyr Phe Tyr Val Gly Leu Asp Tyr Xaa Pro Ala Phe Ser Lys Ile Asn 
1               5                   10                  15      


Gly Phe Glu Ile Arg Glu Ser Thr Gly Glu Thr Ala Ala Val Tyr Pro 
            20                  25                  30          


Tyr Met Lys Asp Gly Thr Arg Val Glu Trp Lys Ala Glu Lys Phe Asp 
        35                  40                  45              


Trp Asn Thr Pro Asp Pro Arg Ile Lys Phe Lys Asn Asn Pro Ile Val 
    50                  55                  60                  


Ala Leu Glu Gly Ser Val Gly Tyr Ser Ile Gly Val Ala Arg Val Glu 
65                  70                  75                  80  


Leu Glu Ile Gly Tyr Glu Gln Phe Lys Thr Lys Gly Ile Arg Asp Thr 
                85                  90                  95      


Gly Ser Lys Glu Glu Glu Ala Asp Ala Val Tyr Leu Leu Ala Lys Lys 
            100                 105                 110         


Leu Pro His Thr Leu Val Ser Asp Gln Ser Asp Lys Phe Leu Glu Glu 
        115                 120                 125             


Leu Lys Asn Thr Lys Ala Ala Glu Ile Val Lys Phe Ala Glu Ala Val 
    130                 135                 140                 


Gly Thr Ser Ala Lys Asp Ile Asp Xaa Lys Val Cys Lys Lys Xaa Xaa 
145                 150                 155                 160 


Xaa Asn Ala Ala Xaa Ser Trp Xaa Cys Xaa Gln Xaa Gly Ser Xaa Xaa 
                165                 170                 175     


Xaa Xaa Xaa Xaa Lys Xaa Xaa Ser Xaa Xaa Phe Thr Lys Ala Gly Val 
            180                 185                 190         


Xaa Xaa Xaa Xaa Xaa Gly Lys Ala Trp Pro Asn Gly Xaa Xaa Xaa Xaa 
        195                 200                 205             


Ala Ala Lys Ala Glu Asp Leu Ser Thr Ala Leu Asn Arg Glu Leu Thr 
    210                 215                 220                 


Ser Ala Glu Lys Asn Lys Val Ala Gly Leu Leu Thr Arg Thr Ile Ser 
225                 230                 235                 240 


Gly Gly Glu Val Val Glu Ile Arg Ala Val Ser Thr Thr Ser Val Met 
                245                 250                 255     


Xaa Asn Ala Cys Tyr Asp Leu Leu Ser 
            260                 265 


<210>  13
<211>  28
<212>  PRT
<213>  Anaplasma playts

<400>  13

Arg Val Glu Leu Glu Ile Gly Tyr Glu Gln Phe Lys Thr Lys Gly Ile 
1               5                   10                  15      


Arg Asp Thr Gly Ser Lys Glu Glu Glu Ala Asp Ala 
            20                  25              


<210>  14
<211>  27
<212>  PRT
<213>  Anaplasma playts

<400>  14

Cys Lys Asp Gly Thr Arg Val Glu Trp Lys Ala Glu Lys Phe Asp Trp 
1               5                   10                  15      


Asn Thr Pro Asp Pro Arg Ile Lys Pro Lys Asn 
            20                  25          


<210>  15
<211>  23
<212>  PRT
<213>  Anaplasma playts

<400>  15

Cys Lys Asp Gly Thr Arg Val Glu Trp Lys Ala Glu Lys Phe Asp Trp 
1               5                   10                  15      


Asn Thr Pro Asp Pro Arg Ile 
            20              


<210>  16
<211>  795
<212>  DNA
<213>  Anaplasma playts

<400>  16
tatttttatg ttggtytgga ytatwgcccg gcgtttagta agataaatgg gtttgagata     60

agagagagta ccggggaaac tgcggcagta tatccgtaca tgaaagatgg aactagagtg    120

gagtggaaag ctgagaagtt cgactggaac acaccagatc cgaggattaa gtttaaaaac    180

aatcctatcg tagcgttgga aggaagtgtg ggctacagta tcggggtagc gagagtagaa    240

ctggagatcg gctatgaaca gttcaagacg aaaggaataa gagatacggg aagtaaggaa    300

gaagaagctg atgccgtgta cctgttggct aagaagctac cgcataccct ggtgagtgac    360

cagagcgata aattcctgga ggagctgaag aatacgaaag cggcggagat cgttaaattt    420

gctgaggctg ttggcacatc ggcaaaggat attgataaga aggtttgtaa gaagcacact    480

aacaatgcgg cgaacagttg gaagtgcgag cagcctggaa gcggcaccga gacaagcgcc    540

aaggcgttca gtgaaatatt tacgaaggca ggcgtaaata ctgacggcaa aggcaaagca    600

tggcctaacg ggcacaccga cagcgccgcg aaagcggaag acctaagtac ggcgttgaat    660

agagaactaa ccagcgccga aaagaacaag gtagctgggc tgctaaccag gactatatcc    720

ggtggtgagg tagtggagat ccgtgcggtg tcgacaacgt cagtaatgwt aaatgcgtgy    780

tacgatctgc tgagc                                                     795


<210>  17
<211>  777
<212>  DNA
<213>  Anaplasma playts

<400>  17
tatttttatg ttggtttgga ttattgcccg gcgtttagta agataaatgg gtttgagata     60

agagagagta ccggggaaac tgcggcagta tatccgtaca tgaaagatgg aactagagtg    120

gagtggaaag ctgagaagtt cgactggaac acaccagatc cgaggattaa gtttaaaaac    180

aatcctatcg tagcgttaga aggaagtgtg ggctacagta tcggggtagc gagagtagaa    240

ctggagatcg gctatgaaca gttcaagacg aaaggaataa gagatacggg aagtaaggaa    300

gaagaagctg atgccgtgta cctgttggct aagaagctac cgcataccct ggtgagtgac    360

cagagcgata aattcctgga ggagctgaag aatacgaaag cggcggagat cgttaagttt    420

gctgaggctg ttggcacatc ggcaaaggat attgatggaa aggtttgtaa gaagcacaac    480

ggcaatgcgg caggcagttg gcagtgcacg cagactggca gcgaaacaag cggcaagacg    540

ttgagtgaga tatttacgaa ggctggcgtg gatgctaacg gcaaagcatg gcctaacgga    600

agcgacgccg cgaaagcgga agacctaagt actgcgttga atagagaact aaccagcgct    660

gaaaagaaca aggtagctgg cctactaacc aggactatat ccggtggcga ggtagtggag    720

atccgtgcgg tgtcgacaac gtcagtaatg ataaacgcat gttacgatct gctgagc       777


<210>  18
<211>  789
<212>  DNA
<213>  Anaplasma playts

<400>  18
tatttttatg ttggtttaga ttatagtccg gcgtttagta agataaatgg gtttgagata     60

agagagagta ccggggaaac tgcggcagta tatccgtaca tgaaagatgg aactagagtg    120

gagtggaaag ctgagaagtt cgactggaac acaccagatc cgaggattaa gtttaaaaac    180

aatcctatcg tagcgttgga aggaagtgtg ggctacagta tcggggtagc gagagtagaa    240

ctggagatcg gctatgaaca gttcaagacg aaaggaataa gagatacggg aagtaaggaa    300

gaagaagctg atgccgtgta cctgttggct aagaagctac cgcataccct ggtgagtgac    360

cagagcgata aattcctgga ggagctgaag aatacgaaag cggcggagat cgttaaattt    420

gctgaggctg ttggcacatc ggcaaaggat attgatggaa aggtttgtaa gaagaacact    480

aacaatgcgg cagacagttg gaagtgcgag cagactggca gcggcagcga cggcaaggag    540

ttcagtaaac tatttacgaa ggctggcgtg gatgctaacg agaaaggcaa agcgtggcct    600

aacgggcaca ccgacagcgc cgcgaaagcg gaagacctaa gtacggcgtt gaatagagaa    660

ctaaccagcg ccgaaaagaa caaggtagct gggctgctaa ccaggactat atccggtggt    720

gaggtagtgg agatccgtgc ggtgtcgaca acgtcagtaa tgttaaacgc ctgctacgat    780

ctgctgagc                                                            789


<210>  19
<211>  293
<212>  PRT
<213>  Anaplasma  phagocytophilum

<400>  19

Tyr Phe Tyr Val Gly Leu Asp Tyr Ser Pro Ala Phe Ser Lys Ile Arg 
1               5                   10                  15      


Asp Phe Ser Ile Arg Glu Ser Asn Gly Glu Thr Lys Ala Val Tyr Pro 
            20                  25                  30          


Tyr Leu Lys Asp Gly Lys Ser Val Lys Leu Glu Ser His Lys Phe Asp 
        35                  40                  45              


Trp Asn Thr Pro Asp Pro Arg Ile Gly Phe Lys Asp Asn Met Leu Val 
    50                  55                  60                  


Ala Met Glu Gly Ser Val Gly Tyr Gly Ile Gly Gly Ala Arg Val Glu 
65                  70                  75                  80  


Leu Glu Ile Gly Tyr Glu Arg Phe Lys Thr Lys Gly Ile Arg Asp Ser 
                85                  90                  95      


Gly Ser Lys Glu Asp Glu Ala Asp Thr Val Tyr Leu Leu Ala Lys Glu 
            100                 105                 110         


Leu Ala Tyr Asp Val Val Thr Gly Gln Thr Asp Asn Leu Ala Ala Ala 
        115                 120                 125             


Leu Ala Lys Thr Ser Gly Lys Asp Ile Val Gln Phe Ala Lys Ala Val 
    130                 135                 140                 


Glu Ile Ser Tyr Pro Ser Ile Asp Gly Lys Val Cys Ser Gly Lys His 
145                 150                 155                 160 


Ala Ala Leu Ala Ala Asn Thr Asn Ala Glu Lys Lys Tyr Ala Val Glu 
                165                 170                 175     


Pro Ala Asn Gly Gly Thr Asp Gly Ser Thr Ser Gln Cys Ser Gly Leu 
            180                 185                 190         


Ser Asn Gly Ser Ala Glu Ala Ala His Lys Tyr Leu Ser Lys Phe Val 
        195                 200                 205             


Ser Leu Thr Gly Val Val Glu Gly Lys Asn Trp Pro Thr Gly Arg Ser 
    210                 215                 220                 


Ser Asn Asn Ser Asn Ser Ile Val Val Gly Ala Pro Asn Ser Asn Ala 
225                 230                 235                 240 


Asn Ala Met Ala Lys Asp Leu Val Lys Glu Leu Thr Pro Glu Glu Lys 
                245                 250                 255     


Thr Ile Val Ala Gly Leu Leu Ala Lys Thr Ile Glu Gly Gly Glu Val 
            260                 265                 270         


Val Glu Ile Arg Ala Val Ser Ser Thr Ser Val Met Val Asn Ala Cys 
        275                 280                 285             


Tyr Asp Leu Leu Ser 
    290             


