                         SEQUENCE LISTING

<110>  The Board of Trustees of the University of Arkansas
       Techno Network Shikoku, Co., Ltd.
       Ochsner Clinic Foundation
       Matsushita, Osamu
       Gensure, Robert C.
       Sakon, Joshua
 
<120>  Fusion protein of collagen-binding domain and parathyroid hormone

<130>  110-020-PCT

<150>  US 60/922,433
<151>  2007-04-09

<160>  12    

<170>  PatentIn version 3.3

<210>  1
<211>  158
<212>  PRT
<213>  Artificial

<220>
<223>  Fusion protein containing parathyroid hormone segment and 
       collagen-binding domain

<400>  1

Ser Val Ser Glu Ile Gln Leu Met His Asn Leu Gly Lys His Leu Asn 
1               5                   10                  15      


Ser Met Glu Arg Val Glu Trp Leu Arg Lys Lys Leu Gln Asp Val His 
            20                  25                  30          


Asn Gly Ile Asn Ser Pro Val Tyr Pro Ile Gly Thr Glu Lys Glu Pro 
        35                  40                  45              


Asn Asn Ser Lys Glu Thr Ala Ser Gly Pro Ile Val Pro Gly Ile Pro 
    50                  55                  60                  


Val Ser Gly Thr Ile Glu Asn Thr Ser Asp Gln Asp Tyr Phe Tyr Phe 
65                  70                  75                  80  


Asp Val Ile Thr Pro Gly Glu Val Lys Ile Asp Ile Asn Lys Leu Gly 
                85                  90                  95      


Tyr Gly Gly Ala Thr Trp Val Val Tyr Asp Glu Asn Asn Asn Ala Val 
            100                 105                 110         


Ser Tyr Ala Thr Asp Asp Gly Gln Asn Leu Ser Gly Lys Phe Lys Ala 
        115                 120                 125             


Asp Lys Pro Gly Arg Tyr Tyr Ile His Leu Tyr Met Phe Asn Gly Ser 
    130                 135                 140                 


Tyr Met Pro Tyr Arg Ile Asn Ile Glu Gly Ser Val Gly Arg 
145                 150                 155             


<210>  2
<211>  251
<212>  PRT
<213>  Artificial

<220>
<223>  Fusion protein containing parathyroid hormone fragment and 
       collagen-binding domain and polycystic kidney disease domain of 
       ColH.

<400>  2

Ser Val Ser Glu Ile Gln Leu Met His Asn Leu Gly Lys His Leu Asn 
1               5                   10                  15      


Ser Met Glu Arg Val Glu Trp Leu Arg Lys Lys Leu Gln Asp Val His 
            20                  25                  30          


Asn Gly Ile Pro Glu Ile Lys Asp Leu Ser Glu Asn Lys Leu Pro Val 
        35                  40                  45              


Ile Tyr Met His Val Pro Lys Ser Gly Ala Leu Asn Gln Lys Val Val 
    50                  55                  60                  


Phe Tyr Gly Lys Gly Thr Tyr Asp Pro Asp Gly Ser Ile Ala Gly Tyr 
65                  70                  75                  80  


Gln Trp Asp Phe Gly Asp Gly Ser Asp Phe Ser Ser Glu Gln Asn Pro 
                85                  90                  95      


Ser His Val Tyr Thr Lys Lys Gly Glu Tyr Thr Val Thr Leu Arg Val 
            100                 105                 110         


Met Asp Ser Ser Gly Gln Met Ser Glu Lys Thr Met Lys Ile Lys Ile 
        115                 120                 125             


Thr Asp Pro Val Tyr Pro Ile Gly Thr Glu Lys Glu Pro Asn Asn Ser 
    130                 135                 140                 


Lys Glu Thr Ala Ser Gly Pro Ile Val Pro Gly Ile Pro Val Ser Gly 
145                 150                 155                 160 


Thr Ile Glu Asn Thr Ser Asp Gln Asp Tyr Phe Tyr Phe Asp Val Ile 
                165                 170                 175     


Thr Pro Gly Glu Val Lys Ile Asp Ile Asn Lys Leu Gly Tyr Gly Gly 
            180                 185                 190         


Ala Thr Trp Val Val Tyr Asp Glu Asn Asn Asn Ala Val Ser Tyr Ala 
        195                 200                 205             


Thr Asp Asp Gly Gln Asn Leu Ser Gly Lys Phe Lys Ala Asp Lys Pro 
    210                 215                 220                 


Gly Arg Tyr Tyr Ile His Leu Tyr Met Phe Asn Gly Ser Tyr Met Pro 
225                 230                 235                 240 


Tyr Arg Ile Asn Ile Glu Gly Ser Val Gly Arg 
                245                 250     


<210>  3
<211>  5464
<212>  DNA
<213>  Artificial

<220>
<223>  Expression vector

<400>  3
agcttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg     60

gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt    120

tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc    180

tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca    240

cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc    300

aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc    360

gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc    420

ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata    480

tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc    540

ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact    600

ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag    660

atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt    720

tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa    780

aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat    840

ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc    900

atcctccaaa atcggatctg atcgaaggtc gttctgtgag tgaaatacag cttatgcata    960

acctgggaaa acatctgaac tcgatggaga gagtagaatg gctgcgtaag aagctgcagg   1020

atgtgcacaa tggaattaat tccccggtat atccaatagg cactgaaaaa gaaccaaata   1080

acagtaaaga aactgcaagt ggtccaatag taccaggtat acctgttagt ggaaccatag   1140

aaaatacaag tgatcaagat tatttctatt ttgatgttat aacaccagga gaagtaaaaa   1200

tagatataaa taaattaggg tacggaggag ctacttgggt agtatatgat gaaaataata   1260

atgcagtatc ttatgccact gatgatgggc aaaatttaag tggaaagttt aaggcagata   1320

aaccaggtag atattacatc catctttaca tgtttaatgg tagttatatg ccatatagaa   1380

ttaatataga aggttcagta ggaagataat attttattag ttgaggtaac tccactcgaa   1440

ttggtcgact cgagcggccg catcgtgact gactgacgat ctgcctcgcg cgtttcggtg   1500

atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag   1560

cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg   1620

gcgcagccat gacccagtca cgtagcgata gcggagtgta taattcttga agacgaaagg   1680

gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt   1740

caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac   1800

attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa   1860

aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat   1920

tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc   1980

agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga   2040

gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg   2100

cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc   2160

agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag   2220

taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc   2280

tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg   2340

taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg   2400

acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac   2460

ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac   2520

cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg   2580

agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg   2640

tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg   2700

agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac   2760

tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg   2820

ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg   2880

tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc   2940

aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc   3000

tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt   3060

agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc   3120

taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact   3180

caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac   3240

agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag   3300

aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg   3360

gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg   3420

tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga   3480

gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt   3540

ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct   3600

ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg   3660

aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac   3720

accgcataaa ttccgacacc atcgaatggt gcaaaacctt tcgcggtatg gcatgatagc   3780

gcccggaaga gagtcaattc agggtggtga atgtgaaacc agtaacgtta tacgatgtcg   3840

cagagtatgc cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg   3900

tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca   3960

accgcgtggc acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca   4020

gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac   4080

tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg   4140

cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg   4200

accaggatgc cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg   4260

tctctgacca gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg   4320

gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa   4380

gttctgtctc ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa   4440

ttcagccgat agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca   4500

tgcaaatgct gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg   4560

cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gatatctcgg   4620

tagtgggata cgacgatacc gaagacagct catgttatat cccgccgtta accaccatca   4680

aacaggattt tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg   4740

gccaggcggt gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc   4800

tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg   4860

cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag   4920

ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga   4980

attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cggattcact   5040

ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct   5100

tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc   5160

ttcccaacag ttgcgcagcc tgaatggcga atggcgcttt gcctggtttc cggcaccaga   5220

agcggtgccg gaaagctggc tggagtgcga tcttcctgag gccgatactg tcgtcgtccc   5280

ctcaaactgg cagatgcacg gttacgatgc gcccatctac accaacgtaa cctatcccat   5340

tacggtcaat ccgccgtttg ttcccacgga gaatccgacg ggttgttact cgctcacatt   5400

taatgttgat gaaagctggc tacaggaagg ccagacgcga attatttttg atggcgttgg   5460

aatt                                                                5464


<210>  4
<211>  383
<212>  PRT
<213>  Artificial

<220>
<223>  GST-PTH-CBD fusion protein

<400>  4

Met Ser Pro Ile Leu Gly Tyr Trp Lys Ile Lys Gly Leu Val Gln Pro 
1               5                   10                  15      


Thr Arg Leu Leu Leu Glu Tyr Leu Glu Glu Lys Tyr Glu Glu His Leu 
            20                  25                  30          


Tyr Glu Arg Asp Glu Gly Asp Lys Trp Arg Asn Lys Lys Phe Glu Leu 
        35                  40                  45              


Gly Leu Glu Phe Pro Asn Leu Pro Tyr Tyr Ile Asp Gly Asp Val Lys 
    50                  55                  60                  


Leu Thr Gln Ser Met Ala Ile Ile Arg Tyr Ile Ala Asp Lys His Asn 
65                  70                  75                  80  


Met Leu Gly Gly Cys Pro Lys Glu Arg Ala Glu Ile Ser Met Leu Glu 
                85                  90                  95      


Gly Ala Val Leu Asp Ile Arg Tyr Gly Val Ser Arg Ile Ala Tyr Ser 
            100                 105                 110         


Lys Asp Phe Glu Thr Leu Lys Val Asp Phe Leu Ser Lys Leu Pro Glu 
        115                 120                 125             


Met Leu Lys Met Phe Glu Asp Arg Leu Cys His Lys Thr Tyr Leu Asn 
    130                 135                 140                 


Gly Asp His Val Thr His Pro Asp Phe Met Leu Tyr Asp Ala Leu Asp 
145                 150                 155                 160 


Val Val Leu Tyr Met Asp Pro Met Cys Leu Asp Ala Phe Pro Lys Leu 
                165                 170                 175     


Val Cys Phe Lys Lys Arg Ile Glu Ala Ile Pro Gln Ile Asp Lys Tyr 
            180                 185                 190         


Leu Lys Ser Ser Lys Tyr Ile Ala Trp Pro Leu Gln Gly Trp Gln Ala 
        195                 200                 205             


Thr Phe Gly Gly Gly Asp His Pro Pro Lys Ser Asp Leu Ile Glu Gly 
    210                 215                 220                 


Arg Ser Val Ser Glu Ile Gln Leu Met His Asn Leu Gly Lys His Leu 
225                 230                 235                 240 


Asn Ser Met Glu Arg Val Glu Trp Leu Arg Lys Lys Leu Gln Asp Val 
                245                 250                 255     


His Asn Gly Ile Asn Ser Pro Val Tyr Pro Ile Gly Thr Glu Lys Glu 
            260                 265                 270         


Pro Asn Asn Ser Lys Glu Thr Ala Ser Gly Pro Ile Val Pro Gly Ile 
        275                 280                 285             


Pro Val Ser Gly Thr Ile Glu Asn Thr Ser Asp Gln Asp Tyr Phe Tyr 
    290                 295                 300                 


Phe Asp Val Ile Thr Pro Gly Glu Val Lys Ile Asp Ile Asn Lys Leu 
305                 310                 315                 320 


Gly Tyr Gly Gly Ala Thr Trp Val Val Tyr Asp Glu Asn Asn Asn Ala 
                325                 330                 335     


Val Ser Tyr Ala Thr Asp Asp Gly Gln Asn Leu Ser Gly Lys Phe Lys 
            340                 345                 350         


Ala Asp Lys Pro Gly Arg Tyr Tyr Ile His Leu Tyr Met Phe Asn Gly 
        355                 360                 365             


Ser Tyr Met Pro Tyr Arg Ile Asn Ile Glu Gly Ser Val Gly Arg 
    370                 375                 380             


<210>  5
<211>  4
<212>  PRT
<213>  Artificial

<220>
<223>  Factor Xa recognition sequence

<400>  5

Ile Glu Gly Arg 
1               


<210>  6
<211>  1021
<212>  PRT
<213>  Clostridium histolyticum

<400>  6

Met Lys Arg Lys Cys Leu Ser Lys Arg Leu Met Leu Ala Ile Thr Met 
1               5                   10                  15      


Ala Thr Ile Phe Thr Val Asn Ser Thr Leu Pro Ile Tyr Ala Ala Val 
            20                  25                  30          


Asp Lys Asn Asn Ala Thr Ala Ala Val Gln Asn Glu Ser Lys Arg Tyr 
        35                  40                  45              


Thr Val Ser Tyr Leu Lys Thr Leu Asn Tyr Tyr Asp Leu Val Asp Leu 
    50                  55                  60                  


Leu Val Lys Thr Glu Ile Glu Asn Leu Pro Asp Leu Phe Gln Tyr Ser 
65                  70                  75                  80  


Ser Asp Ala Lys Glu Phe Tyr Gly Asn Lys Thr Arg Met Ser Phe Ile 
                85                  90                  95      


Met Asp Glu Ile Gly Arg Arg Ala Pro Gln Tyr Thr Glu Ile Asp His 
            100                 105                 110         


Lys Gly Ile Pro Thr Leu Val Glu Val Val Arg Ala Gly Phe Tyr Leu 
        115                 120                 125             


Gly Phe His Asn Lys Glu Leu Asn Glu Ile Asn Lys Arg Ser Phe Lys 
    130                 135                 140                 


Glu Arg Val Ile Pro Ser Ile Leu Ala Ile Gln Lys Asn Pro Asn Phe 
145                 150                 155                 160 


Lys Leu Gly Thr Glu Val Gln Asp Lys Ile Val Ser Ala Thr Gly Leu 
                165                 170                 175     


Leu Ala Gly Asn Glu Thr Ala Pro Pro Glu Val Val Asn Asn Phe Thr 
            180                 185                 190         


Pro Ile Leu Gln Asp Cys Ile Lys Asn Ile Asp Arg Tyr Ala Leu Asp 
        195                 200                 205             


Asp Leu Lys Ser Lys Ala Leu Phe Asn Val Leu Ala Ala Pro Thr Tyr 
    210                 215                 220                 


Asp Ile Thr Glu Tyr Leu Arg Ala Thr Lys Glu Lys Pro Glu Asn Thr 
225                 230                 235                 240 


Pro Trp Tyr Gly Lys Ile Asp Gly Phe Ile Asn Glu Leu Lys Lys Leu 
                245                 250                 255     


Ala Leu Tyr Gly Lys Ile Asn Asp Asn Asn Ser Trp Ile Ile Asp Asn 
            260                 265                 270         


Gly Ile Tyr His Ile Ala Pro Leu Gly Lys Leu His Ser Asn Asn Lys 
        275                 280                 285             


Ile Gly Ile Glu Thr Leu Thr Glu Val Met Lys Val Tyr Pro Tyr Leu 
    290                 295                 300                 


Ser Met Gln His Leu Gln Ser Ala Asp Gln Ile Lys Arg His Tyr Asp 
305                 310                 315                 320 


Ser Lys Asp Ala Glu Gly Asn Lys Ile Pro Leu Asp Lys Phe Lys Lys 
                325                 330                 335     


Glu Gly Lys Glu Lys Tyr Cys Pro Lys Thr Tyr Thr Phe Asp Asp Gly 
            340                 345                 350         


Lys Val Ile Ile Lys Ala Gly Ala Arg Val Glu Glu Glu Lys Val Lys 
        355                 360                 365             


Arg Leu Tyr Trp Ala Ser Lys Glu Val Asn Ser Gln Phe Phe Arg Val 
    370                 375                 380                 


Tyr Gly Ile Asp Lys Pro Leu Glu Glu Gly Asn Pro Asp Asp Ile Leu 
385                 390                 395                 400 


Thr Met Val Ile Tyr Asn Ser Pro Glu Glu Tyr Lys Leu Asn Ser Val 
                405                 410                 415     


Leu Tyr Gly Tyr Asp Thr Asn Asn Gly Gly Met Tyr Ile Glu Pro Glu 
            420                 425                 430         


Gly Thr Phe Phe Thr Tyr Glu Arg Glu Ala Gln Glu Ser Thr Tyr Thr 
        435                 440                 445             


Leu Glu Glu Leu Phe Arg His Glu Tyr Thr His Tyr Leu Gln Gly Arg 
    450                 455                 460                 


Tyr Ala Val Pro Gly Gln Trp Gly Arg Thr Lys Leu Tyr Asp Asn Asp 
465                 470                 475                 480 


Arg Leu Thr Trp Tyr Glu Glu Gly Gly Ala Glu Leu Phe Ala Gly Ser 
                485                 490                 495     


Thr Arg Thr Ser Gly Ile Leu Pro Arg Lys Ser Ile Val Ser Asn Ile 
            500                 505                 510         


His Asn Thr Thr Arg Asn Asn Arg Tyr Lys Leu Ser Asp Thr Val His 
        515                 520                 525             


Ser Lys Tyr Gly Ala Ser Phe Glu Phe Tyr Asn Tyr Ala Cys Met Phe 
    530                 535                 540                 


Met Asp Tyr Met Tyr Asn Lys Asp Met Gly Ile Leu Asn Lys Leu Asn 
545                 550                 555                 560 


Asp Leu Ala Lys Asn Asn Asp Val Asp Gly Tyr Asp Asn Tyr Ile Arg 
                565                 570                 575     


Asp Leu Ser Ser Asn Tyr Ala Leu Asn Asp Lys Tyr Gln Asp His Met 
            580                 585                 590         


Gln Glu Arg Ile Asp Asn Tyr Glu Asn Leu Thr Val Pro Phe Val Ala 
        595                 600                 605             


Asp Asp Tyr Leu Val Arg His Ala Tyr Lys Asn Pro Asn Glu Ile Tyr 
    610                 615                 620                 


Ser Glu Ile Ser Glu Val Ala Lys Leu Lys Asp Ala Lys Ser Glu Val 
625                 630                 635                 640 


Lys Lys Ser Gln Tyr Phe Ser Thr Phe Thr Leu Arg Gly Ser Tyr Thr 
                645                 650                 655     


Gly Gly Ala Ser Lys Gly Lys Leu Glu Asp Gln Lys Ala Met Asn Lys 
            660                 665                 670         


Phe Ile Asp Asp Ser Leu Lys Lys Leu Asp Thr Tyr Ser Trp Ser Gly 
        675                 680                 685             


Tyr Lys Thr Leu Thr Ala Tyr Phe Thr Asn Tyr Lys Val Asp Ser Ser 
    690                 695                 700                 


Asn Arg Val Thr Tyr Asp Val Val Phe His Gly Tyr Leu Pro Asn Glu 
705                 710                 715                 720 


Gly Asp Ser Lys Asn Ser Leu Pro Tyr Gly Lys Ile Asn Gly Thr Tyr 
                725                 730                 735     


Lys Gly Thr Glu Lys Glu Lys Ile Lys Phe Ser Ser Glu Gly Ser Phe 
            740                 745                 750         


Asp Pro Asp Gly Lys Ile Val Ser Tyr Glu Trp Asp Phe Gly Asp Gly 
        755                 760                 765             


Asn Lys Ser Asn Glu Glu Asn Pro Glu His Ser Tyr Asp Lys Val Gly 
    770                 775                 780                 


Thr Tyr Thr Val Lys Leu Lys Val Thr Asp Asp Lys Gly Glu Ser Ser 
785                 790                 795                 800 


Val Ser Thr Thr Thr Ala Glu Ile Lys Asp Leu Ser Glu Asn Lys Leu 
                805                 810                 815     


Pro Val Ile Tyr Met His Val Pro Lys Ser Gly Ala Leu Asn Gln Lys 
            820                 825                 830         


Val Val Phe Tyr Gly Lys Gly Thr Tyr Asp Pro Asp Gly Ser Ile Ala 
        835                 840                 845             


Gly Tyr Gln Trp Asp Phe Gly Asp Gly Ser Asp Phe Ser Ser Glu Gln 
    850                 855                 860                 


Asn Pro Ser His Val Tyr Thr Lys Lys Gly Glu Tyr Thr Val Thr Leu 
865                 870                 875                 880 


Arg Val Met Asp Ser Ser Gly Gln Met Ser Glu Lys Thr Met Lys Ile 
                885                 890                 895     


Lys Ile Thr Asp Pro Val Tyr Pro Ile Gly Thr Glu Lys Glu Pro Asn 
            900                 905                 910         


Asn Ser Lys Glu Thr Ala Ser Gly Pro Ile Val Pro Gly Ile Pro Val 
        915                 920                 925             


Ser Gly Thr Ile Glu Asn Thr Ser Asp Gln Asp Tyr Phe Tyr Phe Asp 
    930                 935                 940                 


Val Ile Thr Pro Gly Glu Val Lys Ile Asp Ile Asn Lys Leu Gly Tyr 
945                 950                 955                 960 


Gly Gly Ala Thr Trp Val Val Tyr Asp Glu Asn Asn Asn Ala Val Ser 
                965                 970                 975     


Tyr Ala Thr Asp Asp Gly Gln Asn Leu Ser Gly Lys Phe Lys Ala Asp 
            980                 985                 990         


Lys Pro Gly Arg Tyr Tyr Ile His  Leu Tyr Met Phe Asn  Gly Ser Tyr 
        995                 1000                 1005             


Met Pro  Tyr Arg Ile Asn Ile  Glu Gly Ser Val Gly  Arg 
    1010                 1015                 1020     


<210>  7
<211>  84
<212>  PRT
<213>  Homo sapiens

<400>  7

Ser Val Ser Glu Ile Gln Leu Met His Asn Leu Gly Lys His Leu Asn 
1               5                   10                  15      


Ser Met Glu Arg Val Glu Trp Leu Arg Lys Lys Leu Gln Asp Val His 
            20                  25                  30          


Asn Phe Val Ala Leu Gly Ala Pro Leu Ala Pro Arg Asp Ala Gly Ser 
        35                  40                  45              


Gln Arg Pro Arg Lys Lys Glu Asp Asn Val Leu Val Glu Ser His Glu 
    50                  55                  60                  


Lys Ser Leu Gly Glu Ala Asp Lys Ala Asp Val Asn Val Leu Thr Lys 
65                  70                  75                  80  


Ala Lys Ser Gln 
                


<210>  8
<211>  141
<212>  PRT
<213>  Homo sapiens

<400>  8

Ala Val Ser Glu His Gln Leu Leu His Asp Lys Gly Lys Ser Ile Gln 
1               5                   10                  15      


Asp Leu Arg Arg Arg Phe Phe Leu His His Leu Ile Ala Glu Ile His 
            20                  25                  30          


Thr Ala Glu Ile Arg Ala Thr Ser Glu Val Ser Pro Asn Ser Lys Pro 
        35                  40                  45              


Ser Pro Asn Thr Lys Asn His Pro Val Arg Phe Gly Ser Asp Asp Glu 
    50                  55                  60                  


Gly Arg Tyr Leu Thr Gln Glu Thr Asn Lys Val Glu Thr Tyr Lys Glu 
65                  70                  75                  80  


Gln Pro Leu Lys Thr Pro Gly Lys Lys Lys Lys Gly Lys Pro Gly Lys 
                85                  90                  95      


Arg Lys Glu Gln Glu Lys Lys Lys Arg Arg Thr Arg Ser Ala Trp Leu 
            100                 105                 110         


Asp Ser Gly Val Thr Gly Ser Gly Leu Glu Gly Asp His Leu Ser Asp 
        115                 120                 125             


Thr Ser Thr Thr Ser Leu Glu Leu Asp Ser Arg Arg His 
    130                 135                 140     


<210>  9
<211>  160
<212>  PRT
<213>  Artificial

<220>
<223>  Gly-Ser-PTH(1-33)-CBD fusion protein

<400>  9

Gly Ser Ser Val Ser Glu Ile Gln Leu Met His Asn Leu Gly Lys His 
1               5                   10                  15      


Leu Asn Ser Met Glu Arg Val Glu Trp Leu Arg Lys Lys Leu Gln Asp 
            20                  25                  30          


Val His Asn Gly Ile Asn Ser Pro Val Tyr Pro Ile Gly Thr Glu Lys 
        35                  40                  45              


Glu Pro Asn Asn Ser Lys Glu Thr Ala Ser Gly Pro Ile Val Pro Gly 
    50                  55                  60                  


Ile Pro Val Ser Gly Thr Ile Glu Asn Thr Ser Asp Gln Asp Tyr Phe 
65                  70                  75                  80  


Tyr Phe Asp Val Ile Thr Pro Gly Glu Val Lys Ile Asp Ile Asn Lys 
                85                  90                  95      


Leu Gly Tyr Gly Gly Ala Thr Trp Val Val Tyr Asp Glu Asn Asn Asn 
            100                 105                 110         


Ala Val Ser Tyr Ala Thr Asp Asp Gly Gln Asn Leu Ser Gly Lys Phe 
        115                 120                 125             


Lys Ala Asp Lys Pro Gly Arg Tyr Tyr Ile His Leu Tyr Met Phe Asn 
    130                 135                 140                 


Gly Ser Tyr Met Pro Tyr Arg Ile Asn Ile Glu Gly Ser Val Gly Arg 
145                 150                 155                 160 


<210>  10
<211>  152
<212>  PRT
<213>  Artificial

<220>
<223>  PTH(7-33)-CBD fusion protein

<400>  10

Leu Met His Asn Leu Gly Lys His Leu Asn Ser Met Glu Arg Val Glu 
1               5                   10                  15      


Trp Leu Arg Lys Lys Leu Gln Asp Val His Asn Gly Ile Asn Ser Pro 
            20                  25                  30          


Val Tyr Pro Ile Gly Thr Glu Lys Glu Pro Asn Asn Ser Lys Glu Thr 
        35                  40                  45              


Ala Ser Gly Pro Ile Val Pro Gly Ile Pro Val Ser Gly Thr Ile Glu 
    50                  55                  60                  


Asn Thr Ser Asp Gln Asp Tyr Phe Tyr Phe Asp Val Ile Thr Pro Gly 
65                  70                  75                  80  


Glu Val Lys Ile Asp Ile Asn Lys Leu Gly Tyr Gly Gly Ala Thr Trp 
                85                  90                  95      


Val Val Tyr Asp Glu Asn Asn Asn Ala Val Ser Tyr Ala Thr Asp Asp 
            100                 105                 110         


Gly Gln Asn Leu Ser Gly Lys Phe Lys Ala Asp Lys Pro Gly Arg Tyr 
        115                 120                 125             


Tyr Ile His Leu Tyr Met Phe Asn Gly Ser Tyr Met Pro Tyr Arg Ile 
    130                 135                 140                 


Asn Ile Glu Gly Ser Val Gly Arg 
145                 150         


<210>  11
<211>  35
<212>  PRT
<213>  Artificial

<220>
<223>  PTH(1-33) with Gly-Ser amino terminal extension

<400>  11

Gly Ser Ser Val Ser Glu Ile Gln Leu Met His Asn Leu Gly Lys His 
1               5                   10                  15      


Leu Asn Ser Met Glu Arg Val Glu Trp Leu Arg Lys Lys Leu Gln Asp 
            20                  25                  30          


Val His Asn 
        35  


<210>  12
<211>  6
<212>  PRT
<213>  Artificial

<220>
<223>  Thrombin cleavage sequence

<400>  12

Leu Val Pro Arg Gly Ser 
1               5       


