
                                SEQUENCE LISTING

<110> Chen, Irvin S.Y.
      An, Dong Sung
      Lois-Caballe, Carlos
      Baltimore, David
      Qin, Xiao-Feng

<120> METHOD FOR EXPRESSION OF SMALL ANTIVIRAL
  RNA MOLECULES WITH REDUCED CYTOTOXICITY WITHIN A CELL
  

<130> CALTE.011CP2

<150> 10/319,341          
<151> 2002-12-12  

<150> 10/243,553          
<151> 2002-09-13  

<150> 60/322,031          
<151> 2001-09-13  

<150> 60/347,782          
<151> 2002-01-09  

<150> 60/389,592          
<151> 2002-06-18  

<150> 60/406,436          
<151> 2002-08-27  

<160> 39

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 56
<212> DNA
<213> Artificial Sequence

<220> 
<223> This represents an anti-human specific siRNA
      cassette comprising human sequence and synthetic
      linker, loop and terminator sequences.

<400> 1
accgagcatg actgacatct acttcaagag agtagatgtc agtcatgctc tttttc     56

<210> 2
<211> 50
<212> DNA
<213> Artificial Sequence

<220> 
<223> This represents an anti-human immune deficiency
      virus specific siRNA cassette comprising human
      immune deficiency virus sequence and synthetic
      linker, loop and terminator sequences.

<400> 2
ggtggagaga gagacattca agagatgtct ctctctccac cttctttttc            50

<210> 3
<211> 9941
<212> DNA
<213> Artificial Sequence

<220> 
<223> This sequence represents a lentiviral vector
      comprising a human immunodeficiency virus flap
      sequence, a green fluorescent protein variant
      sequence, a human ubiquitin promoter sequence and
      a woodchuck hepatitis regulator element sequence.

<400> 3
gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 60
atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 120
gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 180
tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 240
attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 300
atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 360
acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 420
tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 480
tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 540
attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 600
tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 660
ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 720
accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 780
gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 840
ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 900
aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 960
tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1020
gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1080
ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1140
ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1200
ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1260
aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1320
tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1380
caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1440
aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1500
aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 1560
agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 1620
gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 1680
ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 1740
acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 1800
ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 1860
ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 1920
tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 1980
aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 2040
aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 2100
aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 2160
acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 2220
agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 2280
tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 2340
gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 2400
gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 2460
tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 2520
agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 2580
agatccagtt tggttaatta agggtgcagc ggcctccgcg ccgggttttg gcgcctcccg 2640
cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga cgaagggcgc aggagcgttc 2700
ctgatccttc cgcccggacg ctcaggacag cggcccgctg ctcataagac tcggccttag 2760
aaccccagta tcagcagaag gacattttag gacgggactt gggtgactct agggcactgg 2820
ttttctttcc agagagcgga acaggcgagg aaaagtagtc ccttctcggc gattctgcgg 2880
agggatctcc gtggggcggt gaacgccgat gattatataa ggacgcgccg ggtgtggcac 2940
agctagttcc gtcgcagccg ggatttgggt cgcggttctt gtttgtggat cgctgtgatc 3000
gtcacttggt gagttgcggg ctgctgggct ggccggggct ttcgtggccg ccgggccgct 3060
cggtgggacg gaagcgtgtg gagagaccgc caagggctgt agtctgggtc cgcgagcaag 3120
gttgccctga actgggggtt ggggggagcg cacaaaatgg cggctgttcc cgagtcttga 3180
atggaagacg cttgtaaggc gggctgtgag gtcgttgaaa caaggtgggg ggcatggtgg 3240
gcggcaagaa cccaaggtct tgaggccttc gctaatgcgg gaaagctctt attcgggtga 3300
gatgggctgg ggcaccatct ggggaccctg acgtgaagtt tgtcactgac tggagaactc 3360
gggtttgtcg tctggttgcg ggggcggcag ttatgcggtg ccgttgggca gtgcacccgt 3420
acctttggga gcgcgcgcct cgtcgtgtcg tgacgtcacc cgttctgttg gcttataatg 3480
cagggtgggg ccacctgccg gtaggtgtgc ggtaggcttt tctccgtcgc aggacgcagg 3540
gttcgggcct agggtaggct ctcctgaatc gacaggcgcc ggacctctgg tgaggggagg 3600
gataagtgag gcgtcagttt ctttggtcgg ttttatgtac ctatcttctt aagtagctga 3660
agctccggtt ttgaactatg cgctcggggt tggcgagtgt gttttgtgaa gttttttagg 3720
caccttttga aatgtaatca tttgggtcaa tatgtaattt tcagtgttag actagtaaag 3780
cttctgcagg tcgactctag aaaattgtcc gctaaattct ggccgttttt ggcttttttg 3840
ttagacagga tccccgggta ccggtcgcca ccatggtgag caagggcgag gagctgttca 3900
ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg 3960
tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca 4020
ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc 4080
agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc 4140
ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc 4200
gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg 4260
acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca 4320
acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc 4380
acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg 4440
gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca 4500
aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga 4560
tcactctcgg catggacgag ctgtacaagt aaagcggccg cgactctaga attcgatatc 4620
aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt 4680
aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct 4740
attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt 4800
tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac 4860
gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct 4920
ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca 4980
ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt 5040
ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc 5100
ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct 5160
cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg 5220
catcgatacc gtcgacctcg agacctagaa aaacatggag caatcacaag tagcaataca 5280
gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt 5340
ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc 5400
cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat 5460
atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca 5520
ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt 5580
gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg 5640
agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc 5700
ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta 5760
gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa 5820
taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac 5880
tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac 5940
ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc 6000
cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga 6060
aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga 6120
cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat 6180
ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag 6240
cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag 6300
cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt 6360
tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca 6420
cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata 6480
gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca 6540
aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc 6600
gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt 6660
ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt 6720
atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca 6780
gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta 6840
actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 6900
ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag 6960
tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata 7020
tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca 7080
tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg 7140
tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct 7200
cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca 7260
tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg 7320
gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct 7380
ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg 7440
acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag 7500
atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg 7560
ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact 7620
tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata 7680
aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc 7740
atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc 7800
ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt 7860
gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc 7920
ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg 7980
ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct 8040
cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 8100
cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 8160
accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 8220
acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 8280
cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 8340
acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 8400
atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 8460
agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 8520
acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 8580
gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg 8640
gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 8700
gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 8760
gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 8820
acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 8880
tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 8940
ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 9000
catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 9060
ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 9120
caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 9180
ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 9240
tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 9300
cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 9360
aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 9420
tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 9480
gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 9540
cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa 9600
aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 9660
tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 9720
tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 9780
gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 9840
atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 9900
taggggttcc gcgcacattt ccccgaaaag tgccacctga c                     9941



<210> 4
<211> 334
<212> DNA
<213> Homo sapiens

<400> 4
ccccagtgga aagacgcgca ggcaaaacgc accacgtgac ggagcgtgac cgcgcgccga 60
gcgcgcgcca aggtcgggca ggaagagggc ctatttccca tgattccttc atatttgcat 120
atacgataca aggctgttag agagataatt agaattaatt tgactgtaaa cacaaagata 180
ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa 240
ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg 300
gctttatata tcttgtggaa aggacgaaac accg                             334

<210> 5
<211> 246
<212> DNA
<213> Homo sapiens

<400> 5
tctagaccat ggaattcgaa cgctgacgtc atcaacccgc tccaaggaat cgcgggccca 60
gtgtcactag gcgggaacac ccagcgcgcg tgcgccctgg caggaagatg gctgtgaggg 120
acaggggagt ggcgccctgc aatatttgca tgtcgctatg tgttctggga aatcaccata 180
aacgtgaaat gtctttggat ttgggaatct tataagttct gtatgagacc acggatccaa 240
aagctt                                                            246

<210> 6
<211> 355
<212> DNA
<213> Artificial Sequence

<220> 
<223> This represents a mutant human sequence having an
      introduced bacterial tetO1 binding site.

<400> 6
gggaattccc ccagtggaaa gacgcgcagg caaaacgcac cacgtgacgg agcgtgaccg 60
cgcgccgagc ccaaggtcgg gcaggaagag ggcctatttc ccatgattcc ttcatatttg 120
catatacgat acaaggctgt tagagagata attagaatta atttgactgt aaacacaaag 180
atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta 240
aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta ctctatcatt 300
gatagagtta tatatcttgt ggaaaggacg aaacaccgtg gtcttcaagc ttccg      355

<210> 7
<211> 634
<212> DNA
<213> E. coli

<400> 7
gctagccacc atgtccagat tagataaaag taaagtgatt aacagcgcat tagagctgct 60
taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc taggtgtaga 120
gcagcctaca ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg ccttagccat 180
tgagatgtta gataggcacc atactcactt ttgcccttta gaaggggaaa gctggcaaga 240
ttttttacgt aataacgcta aaagttttag atgtgcttta ctaagtcatc gcgatggagc 300
aaaagtacat ttaggtacac ggcctacaga aaaacagtat gaaactctcg aaaatcaatt 360
agccttttta tgccaacaag gtttttcact agagaatgca ttatatgcac tcagcgctgt 420
ggggcatttt actttaggtt gcgtattgga agatcaagag catcaagtcg ctaaagaaga 480
aagggaaaca cctactactg atagtatgcc gccattatta cgacaagcta tcgaattatt 540
tgatcaccaa ggtgcagagc cagccttctt attcggcctt gaattgatca tatgcggatt 600
agaaaaacaa cttaaatgtg aaagtgggtc ttaa                             634

<210> 8
<211> 60
<212> DNA
<213> Artificial Sequence

<220> 
<223> This represents an anti-human specific siRNA
      cassette comprising human sequence and synthetic
      linker, loop and terminator sequences.

<400> 8
gatccccgaa gatacacagc agttgtttca agagaacaac tgctgtgtat cttctttttc 60


<210> 9
<211> 60
<212> DNA
<213> Artificial Sequence

<220> 
<223> This represents an anti-human specific siRNA
      cassette comprising human sequence and synthetic
      linker, loop and terminator sequences.

<400> 9
gatccccgta ccgtgtctgc aatgtgttca agagacacat tgcagacacg gtactttttc 60


<210> 10
<211> 246
<212> DNA
<213> Homo sapiens

<400> 10
tctagaccat ggaattcgaa cgctgacgtc atcaacccgc tccaaggaat cgcgggccca 60
gtgtcactag gcgggaacac ccagcgcgcg tgcgccctgg caggaagatg gctgtgaggg 120
acaggggagt ggcgccctgc aatatttgca tgtcgctatg tgttctggga aatcaccata 180
aacgtgaaat gtctttggat ttgggaatct tataagttct gtatgagacc acggatccaa 240
aagctt                                                            246

<210> 11
<211> 348
<212> DNA
<213> Homo sapiens

<400> 11
atggcaggaa gaagcggaga cagcgacgaa gagctcatca gaacagtcag actcatcaag 60
cttctctatc aaagcccacc tcccaatccc gaggggaccc gacaggcccg aaggaataga 120
agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatccttagc 180
acttatctgg gacgatctgc ggagcctgtg cctcttcagc taccaccgct tgagagactt 240
actcttgatt gtaacgagga ttgtggaact tctgggacgc agggggtggg aagccctcaa 300
atattggtgg aatctcctac agtattggag tcaggaacta aagaatag              348

<210> 12
<211> 348
<212> DNA
<213> Artificial Sequence

<220> 
<223> This sequence represents mutant human sequence.

<400> 12
atggcaggaa gaagcggaga cagcgacgaa gagctcatca gaacagtcag actcatcaag 60
cttctctatc aaagcccacc tcccaatccc gaggggaccc gacaggcccg aaggaataga 120
agaagaaggt ggcgtgagag acagagacag atccattcga ttagtgaacg gatccttagc 180
acttatctgg gacgatctgc ggagcctgtg cctcttcagc taccaccgct tgagagactt 240
actcttgatt gtaacgagga ttgtggaact tctgggacgc agggggtggg aagccctcaa 300
atattggtgg aatctcctac agtattggag tcaggaacta aagaatag              348

<210> 13
<211> 19
<212> RNA
<213> Artificial Sequence

<220> 
<223> This sequence represents a target sequence of
      human CCR5 shRNA (hu13) in human CCR5 mRNA.

<400> 13
gugucaaguc caaucuaug                                              19

<210> 14
<211> 49
<212> RNA
<213> Artificial Sequence

<220> 
<223> This sequence is human CCR5 shRNA (hu13).

<400> 14
gugucaaguc caaucuaugu uguccgacca uagauuggac uugacacuu             49

<210> 15
<211> 47
<212> DNA
<213> Artificial Sequence

<220> 
<223> This sequence is human CCR5 shRNA (hu13) in
      plasmid DNA.

<400> 15
gtgtcaagtc caatctatgt tgtccgacca tagattggac ttgacac               47

<210> 16
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> This is the human CCR5 shRNA (hu1005) target
      sequence in human CCR5 mRNA.

<400> 16
gagcaagcuc aguuuacacc                                             20

<210> 17
<211> 51
<212> RNA
<213> Artificial Sequence

<220> 
<223> This is the sequence of human CCR5 shRNA (hu1005).

<400> 17
gagcaagcuc aguuuacacc uuguccgacg guguaaacug agcuugcucu u          51

<210> 18
<211> 49
<212> DNA
<213> Artificial Sequence

<220> 
<223> This is the sequence of human CCR5 shRNA (hu1005)
      in plasmid DNA.

<400> 18
gagcaagctc agtttacacc ttgtccgacg gtgtaaactg agcttgctc             49

<210> 19
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> This is the target sequence of rhesus CCR5 shRNA
      (rh1005) in rhesus CCR5 mRNA.

<400> 19
gagcaaguuc aguuuacacc                                             20

<210> 20
<211> 51
<212> RNA
<213> Artificial Sequence

<220> 
<223> This is the sequence of rhesus CCR5 shRNA
      (rh1005).

<400> 20
gagcaaguuc aguuuacacc uuguccgacg guguaaacug aacuugcucu u          51

<210> 21
<211> 49
<212> DNA
<213> Artificial Sequence

<220> 
<223> This is the sequence of rhesus CCR5 shRNA (rh1005)
      in plasmid DNA.

<400> 21
gagcaagttc agtttacacc ttgtccgacg gtgtaaactg aacttgctc             49

<210> 22
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> This is the sequence of one of the primer pairs
      that can be used to amplify human CCR5 cDNA from
      pBABE. CCR5 plasmid DNA.

<400> 22
gatggattat caagtgtcaa gtcca                                       25

<210> 23
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> This is the sequence of one of the primer pairs
      that can be used to amplify human CCR5 cDNA from
      pBABE. CCR5 plasmid DNA.

<400> 23
gtcacaagcc cacagatatt tcc                                         23

<210> 24
<211> 45
<212> DNA
<213> Artificial Sequence

<220> 
<223> This is the sequence of a hairpin adopter 1 DNA
      linker containing a MmeI restriction enzyme site.

<400> 24
gtcggacaat tgcgacccgc atgctgcggg tcgcaattgt ccgac                 45

<210> 25
<211> 32
<212> DNA
<213> Artificial Sequence

<220> 
<223> This is the sequence of the +strand of an adopter
      2 (Ad2) linker.

<400> 25
ggggatccct tcggtactcc agaccgtgag tc                               32

<210> 26
<211> 18
<212> DNA
<213> Artificial Sequence

<220> 
<223> This is the sequence of the -strand of an adopter
      2 (Ad2) linker. n can be any nucleotide in the
      position.

<220> 
<221> misc_feature   
<222> 17, 18
<223> n = A,T,C or G

<400> 26
taccgaaggg atccccnn                                               18

<210> 27
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> This is the sequence of a primer that can be used
      to subject Ad2 ligated DNA fragments to a primer
      extension reaction.

<400> 27
gactcacggt ctggagtacc gaag                                        24

<210> 28
<211> 61
<212> DNA
<213> Artificial Sequence

<220> 
<223> This is the sequence of a sense strand of a
      hybridized oligoDNA for generation of a rhesus
      CCR4 shRNA expression unit.

<400> 28
gatccccgag caagttcagt ttacaccttg tccgacgtgt aaactgaact tgctcttttt 60
c                                                                 61

<210> 29
<211> 62
<212> DNA
<213> Artificial Sequence

<220> 
<223> This is the sequence of an anti-sense strand of a
      hybridized oligoDNA for generation of a rhesus
      CCR4 shRNA expression unit.

<400> 29
gggctcgttc aagtcaaatg tggaacaggc tgccacattt gacttgaacg agaaaaagag 60
ct                                                                62

<210> 30
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> This is the sequence of the + strand of an oligo
      DNA linker.

<400> 30
cgatacccta ggacggctga cgc                                         23

<210> 31
<211> 19
<212> DNA
<213> Artificial Sequence

<220> 
<223> This is the sequence of the - strand of an oligo
      DNA linker.

<400> 31
gtcgaccgtc ctagggtat                                              19

<210> 32
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> This is the sequence of the sense portion of an
      oligonucletoide probe used for Northern blot
      analysis of siRNA.

<400> 32
gagcaagttc agtttacacc                                             20

<210> 33
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> This is the sequence of the anti-sense portion of
      an oligonucletoide probe used for Northern blot
      analysis of siRNA.

<400> 33
ggtgtaaact gaacttgctc                                             20

<210> 34
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> This is the sequence of a synthetic rhCCR5 siRNA
      from Sigma-Proligo.

<400> 34
gguguaaacu gaacuugcuc                                             20

<210> 35
<211> 52
<212> DNA
<213> Artificial Sequence

<220> 
<223> This is the sequence of an RT stem loop primer
      used to quantitate the levels of the antisense
      strand of siRNA against rhesus CCR5.

<400> 35
gtcgtatcca gtgcagggtc cgaggtattc gcactggata cgacaagagc aa         52

<210> 36
<211> 18
<212> DNA
<213> Artificial Sequence

<220> 
<223> This is the sequence of a forward primer used in a
      real time stem-llop RT-PCR method used to
      quantitate the levels of the antisense strand of
      siRNA against rhesus CCR5.

<400> 36
gcgcggtgta aactgaac                                               18

<210> 37
<211> 16
<212> DNA
<213> Artificial Sequence

<220> 
<223> This is the sequence of a reverse primer used in a
      real time stem-llop RT-PCR method used to
      quantitate the levels of the antisense strand of
      siRNA against rhesus CCR5.

<400> 37
gtgcagggtc cgaggt                                                 16

<210> 38
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> This is the sequence of a probe used in a real
      time stem-llop RT-PCR method used to quantitate
      the levels of the antisense strand of siRNA
      against rhesus CCR5.

<400> 38
amtggatacg acaagagcaa mgb                                         23

<210> 39
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> This is the sequence of a serial diluted synthetic
      22nt antisense strand of siRNA against rhCCR5.

<400> 39
gguguaaacu gaacuugcuc                                             20
