                         SEQUENCE LISTING

<110>  NeurOK Pharma LLC
       Sivov, Igor
       Kulish, Dmitry
 
<120>  NON-INFECTIOUS RECOMBINANT HEPATITIS C VIRUS-LIKE PARTICLES AND 
       THEIR PHARMACEUTICAL APPLICATIONS

<130>  2207942-WO1

<150>  2007108037
<151>  2007-03-05

<150>  2007138092
<151>  2007-10-16

<160>  14    

<170>  PatentIn version 3.3

<210>  1
<211>  747
<212>  DNA
<213>  Cytomegalovirus

<400>  1
tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc     60

ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat    120

gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt    180

acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat    240

ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt    300

cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa    360

actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc    420

aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct    480

acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag    540

tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt    600

gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac    660

aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc    720

agagctctct ggctaactag agaaccc                                        747


<210>  2
<211>  77
<212>  DNA
<213>  Foot-and-mouth disease virus

<400>  2
tcacgccaac gacgactccc gcgtcgcact ccacacttac gtctctgcga gtgtaggaac     60

cgacggactg tcgctca                                                    77


<210>  3
<211>  114
<212>  DNA
<213>  artificial

<220>
<223>  YFV-HIV hybrid IRES used in a "replicon-producing" construct

<400>  3
gtggcgcccg aacagggacc tgaaagcgaa agggaaacca gaggagctct ctcgacgcag     60

gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgag          114


<210>  4
<211>  4107
<212>  DNA
<213>  Foot-and-mouth disease virus

<400>  4
atgttcttct tctccgacgt cagggcgaac ttcacgaagt tggtggacac tgtcaaccag     60

atgcaggagg acatgtcaac gaaacacgga cccgacttca accgactggt gtccgcgttt    120

gaggaattgg ccgctggggt caaggccatc aggaccggtc tcgacgaggc caagccctgg    180

tacaagctca tcaaacttct aagccgcctg tcgtgcatgg ccgctgtggc agcacggtcc    240

aaggacccag tccttgtggc catcatgctg gccgacaccg gcctcgagat tctggacagc    300

accttcgtgg taaagaagat ctccgactcg ctctccagtc tcttccacgt gccggctccc    360

gccttcagtt tcggagcccc gatcctgttg gccgggttgg tcaaagtcgc ctcgagtttc    420

ttccagtcca cacccgaaga cctcgagaga gcagaaaagc agctcaaagc acgtgacatc    480

aacgacatat ttgccgttct taagaacggt gagtggctgg tcaaactgat cctggccatc    540

cgcgactgga ttaaggcatg gatcgcctca gaagagaagt ttgtcaccat gacagacctg    600

gtgcctggca tccttgaaag acaacgggat ctcaatgacc ccggcaaata caaggaggcc    660

aaggaatggc tggacaacgc gcgtcaagcg tgtttgaaga gcgggaacgt gcacattgcc    720

aatctgtgta aggtggtcgc tccggcgccc agcaagtcga gacccgaacc agtggtcgtg    780

tgccttcgcg gcaaatccgg ccaagggaaa agctttctcg cgaacgttct cgcgcaggca    840

atttccacac acttcactgg taggaccgac tcggtctggt actgcccgcc cgaccctgac    900

cactttgacg gttacaatca gcagaccgtc gtcgtgatgg acgacttggg ccaaaaccca    960

gacggcaaag acttcaagta ctttgcccaa atggtatcca ccacggggtt catcccgcct   1020

atggcctcgc tcgaggataa gggtaaaccc ttcaacagca aggtcataat agctacaacc   1080

aacctgtact cgggattcac cccaaagacc atggtgtgcc ccgatgcgct taaccggagg   1140

tttcactttg acatcgacgt gagcgccaaa gacgggtaca agatcaacaa caaactggac   1200

atagtcaaag cacttgaaga cacccacgct aacccggtgg cgatgttcca atacgactgc   1260

gctcttctca acggaatggc cgttgaaatg aagagaatgc agcaagacat gttcaagcct   1320

caaccacccc tccagaacat ctaccagctc gttcaggagg tgattgagcg ggtggaacta   1380

cacgaaaagg tgtcgagcca cctgatattt aaacagattt caatcccttc ccagaagtcc   1440

gtgttgtact tcctcattga gaagggccag cacgaagcag cgatcgagtt cttcgagggg   1500

atggtccacg attccatcaa agaggaactc cgacccctca ttcagcagac ctcgttcgta   1560

aaacgcgcct tcaagcgcct gaaagagaac tttgaagttg tagctctgtg tttgaccctc   1620

ttggcaaaca tagtgattat gctccgccaa gcgcgcaaga ggtaccaatc ggtggatgac   1680

ccactggacg gcgacgtaac tcttggcgac gcggaaaaga accctctgga gacgagtggc   1740

gctagcgctg tcggtttccg agagagatcc cccaccgagc aagggacgcg cgaagacgcg   1800

aacgctgagc ccgtcgtgtt cggtagggaa caaccgcgag ctgaaggacc ctacgctggg   1860

ccactcgagc gtcagaaacc tcttaaagtg aaagccgagc tgccacaaca ggagggacca   1920

tacgccggcc caatggagag acagaaaccg ctaaaggtga aagcaaaagc ccccgtcgtg   1980

aaggaaggac cttacgaggg accggtgaag aaacctgtcg ctttgaaagt gaaagcaaag   2040

aacttgatag tcactgagag tggtgcgcca ccgaccgact tgcaaaagat ggtcatgggc   2100

aacactaagc cagtcgagct catcctcgac ggcaagacgg tagccatttg ctgtgctacc   2160

ggagtgttcg gcactgccta cctcgtgcct cgtcatctct tcgcggaaaa gtacgacaag   2220

atcatgttgg acggcagagc cttgacagac agtgactaca gagtgtttga gtttgagatt   2280

aaagtaaaag gacaggacat gctctcagac gccgctctca tggtgttgca ccgtgggaat   2340

cgcgtgcgtg acatcacgaa acactttcgt gacgtagcga gaatgaagaa gggaaccccc   2400

gtcgtcggtg tgatcaacaa tgctgacgtc gggagactca tattctctgg tgaagccctt   2460

acttacaagg acatcgtcgt gtgtatggat ggagacacca tgcctgggct ctttgcctac   2520

agggcatcca ccaaggcagg ctactgtgga ggagccgtcc tggcaaagga cggggccgaa   2580

acgttcatcg ttggcaccca ctccgcaggt ggaaacggca taggatactg ttcgtgtgtt   2640

tcccgatcaa tgctcctgaa gatgaaggca cacatcgacc ctgaaccaca ccacgagggg   2700

ttgatcgtcg acaccagaga tgtggaggag cgtgtccacg tgatgcgcaa aaccaagctc   2760

gcgcccaccg tggcgcacgg tgtgttcaac cctgagttcg ggcctgccgc tctgtccaac   2820

aaggacccgc gcctgaacga aggggttgtc cttgacgatg tcattttctc caaacacaaa   2880

ggagatacaa ggatgtctga agaggacaaa gcgctgtttc ggcgctgtgc tgctgactac   2940

gcgtcgcgtc tacacagtgt gttggggaca gcaaacgccc cactgagtgt gtatgaagcc   3000

atcaaaggcg tcgacggact tgacgccatg gagccggaca cggcgcccgg tctcccctgg   3060

gctctccaag ggaaacgccg cggcgccctg atcgacttcg aaaacggcac cgtcgggcct   3120

gaggttgagg cagcactcaa gctcatggaa agccgcgagt acaaattcgt ctgccaaacc   3180

ttcctgaagg acgaaattcg gccgctagaa aaggtacgcg ctggcaagac acgcattgtc   3240

gacgtgttgc ctgttgaaca cattctctac accagaatga tgattggcag attctgtgct   3300

cagatgcatt caaacaacgg accgcaaatt ggatcagcgg tcggttgtaa ccctgacgtt   3360

gattggcaaa gatttggcac acatttcgcc cagtacaaaa acgtgtggga tgtggactac   3420

tcagcctttg atgcaaacca ctgcagcgat gcgatgaaca tcatgttcga ggaagtgttc   3480

cgcacggagt tcggattcca cccgaacgcc gagtggattc tgaagactct agtgaacacg   3540

gagcacgctt acgagaacaa gcgcattgtt gttgaaggtg gaatgccgtc cggttgttcc   3600

gcaacaagca tcatcaacac aattttgaac aacatctacg tgctttacgc cctgcgtagg   3660

cactatgagg gagtcgagct ggacacttac accatgatct cttatggaga cgacatcgtg   3720

gtggcaagtg actacgacct ggactttgag gctctcaagc cccacttcaa gtcccttggt   3780

cagactatca ctccggccga caaaagcgac aaaggttttg ttcttggtca ctccataacc   3840

gacgtcactt tcctcaaaag acacttccac atggactacg gaactgggtt ttacaaacct   3900

gtgatggcct cgaagaccct cgaggccatc ctctcctttg cacgccgtgg gaccatacag   3960

gagaagttga tctccgtggc aggactcgcc gtccactccg gacctgatga ataccggcgc   4020

ctctttgagc ccttccaagg cctcttcgag attccaagct acagatcact ttacctgcga   4080

tgggtgaacg ccgtgtgcgg tgacgca                                       4107


<210>  5
<211>  353
<212>  DNA
<213>  Foot-and-mouth disease virus

<400>  5
agctggattg tgcggacaaa cgccgcttca cgcacctcgc gtgaccggcc agtactctta     60

ccactttccg cctacttggt cgtcagcgct gttttgggca ctcctgttgg gggctgttcg    120

acgctccacg gtctcctccg tactgacatc tacggtgttg gggccgccac gtgcgagccg    180

ctcgcctggt gtgcttcgac tgtcacccga cgcccgcctt tcaccccccc ccccctaggt    240

tctaccgtcg ttcccgacgt ttgaagggag gaaaccacac gcttgcaaca ccactcccgg    300

tgtcaacggg atgcaaccgc aagatagccg ttttcgcccg gaagtaaaac ggc           353


<210>  6
<211>  6532
<212>  DNA
<213>  artificial

<220>
<223>  "replicon-producing" construct designed for expression in BHK21 
       cell culture of FMDV replication machinery

<400>  6
atcaatattg gctattggcc attgcatacg ttgtatctat atcataatat gtacatttat     60

attggctcat gtccaatatg accgccatgt tggcattgat tattgactag ttattaatag    120

taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt    180

acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg    240

acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat    300

ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct    360

attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg    420

gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg    480

ttttggcagt acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc    540

caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa    600

tgtcgtaaca actgcgatcg cccgccccgt tgacgcaaat gggcggtagg cgtgtacggt    660

gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcactaga agctttattg    720

cggtagttta tcacagttaa attgctaacg cagtcagtgc ttctgacaca acagtctcga    780

acttaagctg cagtgactct cttaaggtag ccttgcagaa gttggtcgtg aggcactggg    840

caggtaagta tcaaggttac aagacaggtt taaggagacc aatagaaact gggcttgtcg    900

agacagagaa gactcttgcg tttctgatag gcacctattg gtcttactga catccacttt    960

gcctttctct ccacaggtgt ccactcccag ttcaattaca gctcttaagg ctagagtact   1020

taatacgact cactataggc tagcctcgag aattcacgcg tggtacctct agagtcgacc   1080

ctcacgccaa cgacgactcc cgcgtcgcac tccacactta cgtctctgcg agtgtaggaa   1140

ccgacggact gtcgctcaaa gcttgcatgc ctgcaggtcg actctagagg atccccgtgg   1200

cgcccgaaca gggacctgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact   1260

cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag atgttcttct   1320

tctccgacgt cagggcgaac ttcacgaagt tggtggacac tgtcaaccag atgcaggagg   1380

acatgtcaac gaaacacgga cccgacttca accgactggt gtccgcgttt gaggaattgg   1440

ccgctggggt caaggccatc aggaccggtc tcgacgaggc caagccctgg tacaagctca   1500

tcaaacttct aagccgcctg tcgtgcatgg ccgctgtggc agcacggtcc aaggacccag   1560

tccttgtggc catcatgctg gccgacaccg gcctcgagat tctggacagc accttcgtgg   1620

taaagaagat ctccgactcg ctctccagtc tcttccacgt gccggctccc gccttcagtt   1680

tcggagcccc gatcctgttg gccgggttgg tcaaagtcgc ctcgagtttc ttccagtcca   1740

cacccgaaga cctcgagaga gcagaaaagc agctcaaagc acgtgacatc aacgacatat   1800

ttgccgttct taagaacggt gagtggctgg tcaaactgat cctggccatc cgcgactgga   1860

ttaaggcatg gatcgcctca gaagagaagt ttgtcaccat gacagacctg gtgcctggca   1920

tccttgaaag acaacgggat ctcaatgacc ccggcaaata caaggaggcc aaggaatggc   1980

tggacaacgc gcgtcaagcg tgtttgaaga gcgggaacgt gcacattgcc aatctgtgta   2040

aggtggtcgc tccggcgccc agcaagtcga gacccgaacc agtggtcgtg tgccttcgcg   2100

gcaaatccgg ccaagggaaa agctttctcg cgaacgttct cgcgcaggca atttccacac   2160

acttcactgg taggaccgac tcggtctggt actgcccgcc cgaccctgac cactttgacg   2220

gttacaatca gcagaccgtc gtcgtgatgg acgacttggg ccaaaaccca gacggcaaag   2280

acttcaagta ctttgcccaa atggtatcca ccacggggtt catcccgcct atggcctcgc   2340

tcgaggataa gggtaaaccc ttcaacagca aggtcataat agctacaacc aacctgtact   2400

cgggattcac cccaaagacc atggtgtgcc ccgatgcgct taaccggagg tttcactttg   2460

acatcgacgt gagcgccaaa gacgggtaca agatcaacaa caaactggac atagtcaaag   2520

cacttgaaga cacccacgct aacccggtgg cgatgttcca atacgactgc gctcttctca   2580

acggaatggc cgttgaaatg aagagaatgc agcaagacat gttcaagcct caaccacccc   2640

tccagaacat ctaccagctc gttcaggagg tgattgagcg ggtggaacta cacgaaaagg   2700

tgtcgagcca cctgatattt aaacagattt caatcccttc ccagaagtcc gtgttgtact   2760

tcctcattga gaagggccag cacgaagcag cgatcgagtt cttcgagggg atggtccacg   2820

attccatcaa agaggaactc cgacccctca ttcagcagac ctcgttcgta aaacgcgcct   2880

tcaagcgcct gaaagagaac tttgaagttg tagctctgtg tttgaccctc ttggcaaaca   2940

tagtgattat gctccgccaa gcgcgcaaga ggtaccaatc ggtggatgac ccactggacg   3000

gcgacgtaac tcttggcgac gcggaaaaga accctctgga gacgagtggc gctagcgctg   3060

tcggtttccg agagagatcc cccaccgagc aagggacgcg cgaagacgcg aacgctgagc   3120

ccgtcgtgtt cggtagggaa caaccgcgag ctgaaggacc ctacgctggg ccactcgagc   3180

gtcagaaacc tcttaaagtg aaagccgagc tgccacaaca ggagggacca tacgccggcc   3240

caatggagag acagaaaccg ctaaaggtga aagcaaaagc ccccgtcgtg aaggaaggac   3300

cttacgaggg accggtgaag aaacctgtcg ctttgaaagt gaaagcaaag aacttgatag   3360

tcactgagag tggtgcgcca ccgaccgact tgcaaaagat ggtcatgggc aacactaagc   3420

cagtcgagct catcctcgac ggcaagacgg tagccatttg ctgtgctacc ggagtgttcg   3480

gcactgccta cctcgtgcct cgtcatctct tcgcggaaaa gtacgacaag atcatgttgg   3540

acggcagagc cttgacagac agtgactaca gagtgtttga gtttgagatt aaagtaaaag   3600

gacaggacat gctctcagac gccgctctca tggtgttgca ccgtgggaat cgcgtgcgtg   3660

acatcacgaa acactttcgt gacgtagcga gaatgaagaa gggaaccccc gtcgtcggtg   3720

tgatcaacaa tgctgacgtc gggagactca tattctctgg tgaagccctt acttacaagg   3780

acatcgtcgt gtgtatggat ggagacacca tgcctgggct ctttgcctac agggcatcca   3840

ccaaggcagg ctactgtgga ggagccgtcc tggcaaagga cggggccgaa acgttcatcg   3900

ttggcaccca ctccgcaggt ggaaacggca taggatactg ttcgtgtgtt tcccgatcaa   3960

tgctcctgaa gatgaaggca cacatcgacc ctgaaccaca ccacgagggg ttgatcgtcg   4020

acaccagaga tgtggaggag cgtgtccacg tgatgcgcaa aaccaagctc gcgcccaccg   4080

tggcgcacgg tgtgttcaac cctgagttcg ggcctgccgc tctgtccaac aaggacccgc   4140

gcctgaacga aggggttgtc cttgacgatg tcattttctc caaacacaaa ggagatacaa   4200

ggatgtctga agaggacaaa gcgctgtttc ggcgctgtgc tgctgactac gcgtcgcgtc   4260

tacacagtgt gttggggaca gcaaacgccc cactgagtgt gtatgaagcc atcaaaggcg   4320

tcgacggact tgacgccatg gagccggaca cggcgcccgg tctcccctgg gctctccaag   4380

ggaaacgccg cggcgccctg atcgacttcg aaaacggcac cgtcgggcct gaggttgagg   4440

cagcactcaa gctcatggaa agccgcgagt acaaattcgt ctgccaaacc ttcctgaagg   4500

acgaaattcg gccgctagaa aaggtacgcg ctggcaagac acgcattgtc gacgtgttgc   4560

ctgttgaaca cattctctac accagaatga tgattggcag attctgtgct cagatgcatt   4620

caaacaacgg accgcaaatt ggatcagcgg tcggttgtaa ccctgacgtt gattggcaaa   4680

gatttggcac acatttcgcc cagtacaaaa acgtgtggga tgtggactac tcagcctttg   4740

atgcaaacca ctgcagcgat gcgatgaaca tcatgttcga ggaagtgttc cgcacggagt   4800

tcggattcca cccgaacgcc gagtggattc tgaagactct agtgaacacg gagcacgctt   4860

acgagaacaa gcgcattgtt gttgaaggtg gaatgccgtc cggttgttcc gcaacaagca   4920

tcatcaacac aattttgaac aacatctacg tgctttacgc cctgcgtagg cactatgagg   4980

gagtcgagct ggacacttac accatgatct cttatggaga cgacatcgtg gtggcaagtg   5040

actacgacct ggactttgag gctctcaagc cccacttcaa gtcccttggt cagactatca   5100

ctccggccga caaaagcgac aaaggttttg ttcttggtca ctccataacc gacgtcactt   5160

tcctcaaaag acacttccac atggactacg gaactgggtt ttacaaacct gtgatggcct   5220

cgaagaccct cgaggccatc ctctcctttg cacgccgtgg gaccatacag gagaagttga   5280

tctccgtggc aggactcgcc gtccactccg gacctgatga ataccggcgc ctctttgagc   5340

ccttccaagg cctcttcgag attccaagct acagatcact ttacctgcga tgggtgaacg   5400

ccgtgtgcgg tgacgcacgt cgcaaacgta gcaagggcga ggaactgttc actggcgtgg   5460

tcccaattct cgtggaactg gatggcgatg tgaatgggca caaattttct gtcagcggag   5520

agggtgaagg tgatgccaca tacggaaagc tcaccctgaa attcatctgc accactggaa   5580

agctccctgt gccatggcca acactggtca ctaccttcwc yyatggcgtg cagtgctttt   5640

ccagataccc agaccatatg aagcagcatg actttttcaa gagcgccatg cccgagggct   5700

atgtgcagga gagaaccatc tttttcaaag atgacgggaa ctacaagacc cgcgctgaag   5760

tcaagttcga aggtgacacc ctggtgaata gaatcgagtt gaagggcatt gactttaagg   5820

aagatggaaa cattctcggc cacaagctgg aatacaacta taactcccac aatgtgtaca   5880

tcatggccga caagcaaaag aatggcatca aggtcaactt caagatcaga cacaacattg   5940

aggatggatc cgtgcagctg gccgaccatt atcaacagaa cactccaatc ggcgacggcc   6000

ctgtgctcct cccagacaac cattacctgt ccacccagtc tgccctgtct aaagatccca   6060

acgaaaagag agaccacatg gtcctgctgg agtttgtgac cgctgctggg atcacacatg   6120

gcatggacga gctgtacaag taaaaatttt gactagcgga ggctagaagg agagagtaga   6180

gctggattgt gcggacaaac gccgcttcac gcacctcgcg tgaccggcca gtactcttac   6240

cactttccgc ctacttggtc gtcagcgctg ttttgggcac tcctgttggg ggctgttcga   6300

cgctccacgg tctcctccgt actgacatct acggtgttgg ggccgccacg tgcgagccgc   6360

tcgcctggtg tgcttcgact gtcacccgac gcccgccttt cacccccccc cccctaggtt   6420

ctaccgtcgt tcccgacgtt tgaagggagg aaaccacacg cttgcaacac cactcccggt   6480

gtcaacggga tgcaaccgca agatagccgt tttcgcccgg aagtaaaacg gc           6532


<210>  7
<211>  5753
<212>  DNA
<213>  artificial

<220>
<223>  "DKIS-FUN": fragment of pDKIS-0107 comprising FMDV-3'-UTR, 
       YFH-HIV hybrid IRES, FMDV non-structural (NS) polyprotein, and 
       FMDV-5'-UTR

<400>  7
cagtctctgt cgccctcacg ccaacgacga ctcccgcgtc gcactccaca cttacgtctc     60

tgcgagtgta ggaaccgacg gactgtcgct caaagcttgc atgcctgcag gtcgactcta    120

gaggatcccc gtggcgcccg aacagggacc tgaaagcgaa agggaaacca gaggagctct    180

ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg    240

tgagatgttc ttcttctccg acgtcagggc gaacttcacg aagttggtgg acactgtcaa    300

ccagatgcag gaggacatgt caacgaaaca cggacccgac ttcaaccgac tggtgtccgc    360

gtttgaggaa ttggccgctg gggtcaaggc catcaggacc ggtctcgacg aggccaagcc    420

ctggtacaag ctcatcaaac ttctaagccg cctgtcgtgc atggccgctg tggcagcacg    480

gtccaaggac ccagtccttg tggccatcat gctggccgac accggcctcg agattctgga    540

cagcaccttc gtggtaaaga agatctccga ctcgctctcc agtctcttcc acgtgccggc    600

tcccgccttc agtttcggag ccccgatcct gttggccggg ttggtcaaag tcgcctcgag    660

tttcttccag tccacacccg aagacctcga gagagcagaa aagcagctca aagcacgtga    720

catcaacgac atatttgccg ttcttaagaa cggtgagtgg ctggtcaaac tgatcctggc    780

catccgcgac tggattaagg catggatcgc ctcagaagag aagtttgtca ccatgacaga    840

cctggtgcct ggcatccttg aaagacaacg ggatctcaat gaccccggca aatacaagga    900

ggccaaggaa tggctggaca acgcgcgtca agcgtgtttg aagagcggga acgtgcacat    960

tgccaatctg tgtaaggtgg tcgctccggc gcccagcaag tcgagacccg aaccagtggt   1020

cgtgtgcctt cgcggcaaat ccggccaagg gaaaagcttt ctcgcgaacg ttctcgcgca   1080

ggcaatttcc acacacttca ctggtaggac cgactcggtc tggtactgcc cgcccgaccc   1140

tgaccacttt gacggttaca atcagcagac cgtcgtcgtg atggacgact tgggccaaaa   1200

cccagacggc aaagacttca agtactttgc ccaaatggta tccaccacgg ggttcatccc   1260

gcctatggcc tcgctcgagg ataagggtaa acccttcaac agcaaggtca taatagctac   1320

aaccaacctg tactcgggat tcaccccaaa gaccatggtg tgccccgatg cgcttaaccg   1380

gaggtttcac tttgacatcg acgtgagcgc caaagacggg tacaagatca acaacaaact   1440

ggacatagtc aaagcacttg aagacaccca cgctaacccg gtggcgatgt tccaatacga   1500

ctgcgctctt ctcaacggaa tggccgttga aatgaagaga atgcagcaag acatgttcaa   1560

gcctcaacca cccctccaga acatctacca gctcgttcag gaggtgattg agcgggtgga   1620

actacacgaa aaggtgtcga gccacctgat atttaaacag atttcaatcc cttcccagaa   1680

gtccgtgttg tacttcctca ttgagaaggg ccagcacgaa gcagcgatcg agttcttcga   1740

ggggatggtc cacgattcca tcaaagagga actccgaccc ctcattcagc agacctcgtt   1800

cgtaaaacgc gccttcaagc gcctgaaaga gaactttgaa gttgtagctc tgtgtttgac   1860

cctcttggca aacatagtga ttatgctccg ccaagcgcgc aagaggtacc aatcggtgga   1920

tgacccactg gacggcgacg taactcttgg cgacgcggaa aagaaccctc tggagacgag   1980

tggcgctagc gctgtcggtt tccgagagag atcccccacc gagcaaggga cgcgcgaaga   2040

cgcgaacgct gagcccgtcg tgttcggtag ggaacaaccg cgagctgaag gaccctacgc   2100

tgggccactc gagcgtcaga aacctcttaa agtgaaagcc gagctgccac aacaggaggg   2160

accatacgcc ggcccaatgg agagacagaa accgctaaag gtgaaagcaa aagcccccgt   2220

cgtgaaggaa ggaccttacg agggaccggt gaagaaacct gtcgctttga aagtgaaagc   2280

aaagaacttg atagtcactg agagtggtgc gccaccgacc gacttgcaaa agatggtcat   2340

gggcaacact aagccagtcg agctcatcct cgacggcaag acggtagcca tttgctgtgc   2400

taccggagtg ttcggcactg cctacctcgt gcctcgtcat ctcttcgcgg aaaagtacga   2460

caagatcatg ttggacggca gagccttgac agacagtgac tacagagtgt ttgagtttga   2520

gattaaagta aaaggacagg acatgctctc agacgccgct ctcatggtgt tgcaccgtgg   2580

gaatcgcgtg cgtgacatca cgaaacactt tcgtgacgta gcgagaatga agaagggaac   2640

ccccgtcgtc ggtgtgatca acaatgctga cgtcgggaga ctcatattct ctggtgaagc   2700

ccttacttac aaggacatcg tcgtgtgtat ggatggagac accatgcctg ggctctttgc   2760

ctacagggca tccaccaagg caggctactg tggaggagcc gtcctggcaa aggacggggc   2820

cgaaacgttc atcgttggca cccactccgc aggtggaaac ggcataggat actgttcgtg   2880

tgtttcccga tcaatgctcc tgaagatgaa ggcacacatc gaccctgaac cacaccacga   2940

ggggttgatc gtcgacacca gagatgtgga ggagcgtgtc cacgtgatgc gcaaaaccaa   3000

gctcgcgccc accgtggcgc acggtgtgtt caaccctgag ttcgggcctg ccgctctgtc   3060

caacaaggac ccgcgcctga acgaaggggt tgtccttgac gatgtcattt tctccaaaca   3120

caaaggagat acaaggatgt ctgaagagga caaagcgctg tttcggcgct gtgctgctga   3180

ctacgcgtcg cgtctacaca gtgtgttggg gacagcaaac gccccactga gtgtgtatga   3240

agccatcaaa ggcgtcgacg gacttgacgc catggagccg gacacggcgc ccggtctccc   3300

ctgggctctc caagggaaac gccgcggcgc cctgatcgac ttcgaaaacg gcaccgtcgg   3360

gcctgaggtt gaggcagcac tcaagctcat ggaaagccgc gagtacaaat tcgtctgcca   3420

aaccttcctg aaggacgaaa ttcggccgct agaaaaggta cgcgctggca agacacgcat   3480

tgtcgacgtg ttgcctgttg aacacattct ctacaccaga atgatgattg gcagattctg   3540

tgctcagatg cattcaaaca acggaccgca aattggatca gcggtcggtt gtaaccctga   3600

cgttgattgg caaagatttg gcacacattt cgcccagtac aaaaacgtgt gggatgtgga   3660

ctactcagcc tttgatgcaa accactgcag cgatgcgatg aacatcatgt tcgaggaagt   3720

gttccgcacg gagttcggat tccacccgaa cgccgagtgg attctgaaga ctctagtgaa   3780

cacggagcac gcttacgaga acaagcgcat tgttgttgaa ggtggaatgc cgtccggttg   3840

ttccgcaaca agcatcatca acacaatttt gaacaacatc tacgtgcttt acgccctgcg   3900

taggcactat gagggagtcg agctggacac ttacaccatg atctcttatg gagacgacat   3960

cgtggtggca agtgactacg acctggactt tgaggctctc aagccccact tcaagtccct   4020

tggtcagact atcactccgg ccgacaaaag cgacaaaggt tttgttcttg gtcactccat   4080

aaccgacgtc actttcctca aaagacactt ccacatggac tacggaactg ggttttacaa   4140

acctgtgatg gcctcgaaga ccctcgaggc catcctctcc tttgcacgcc gtgggaccat   4200

acaggagaag ttgatctccg tggcaggact cgccgtccac tccggacctg atgaataccg   4260

gcgcctcttt gagcccttcc aaggcctctt cgagattcca agctacagat cactttacct   4320

gcgatgggtg aacgccgtgt gcggtgacgc acgtcgcaaa cgtagcaagg gcgaggaact   4380

gttcactggc gtggtcccaa ttctcgtgga actggatggc gatgtgaatg ggcacaaatt   4440

ttctgtcagc ggagagggtg aaggtgatgc cacatacgga aagctcaccc tgaaattcat   4500

ctgcaccact ggaaagctcc ctgtgccatg gccaacactg gtcactacct tcwcyyatgg   4560

cgtgcagtgc ttttccagat acccagacca tatgaagcag catgactttt tcaagagcgc   4620

catgcccgag ggctatgtgc aggagagaac catctttttc aaagatgacg ggaactacaa   4680

gacccgcgct gaagtcaagt tcgaaggtga caccctggtg aatagaatcg agttgaaggg   4740

cattgacttt aaggaagatg gaaacattct cggccacaag ctggaataca actataactc   4800

ccacaatgtg tacatcatgg ccgacaagca aaagaatggc atcaaggtca acttcaagat   4860

cagacacaac attgaggatg gatccgtgca gctggccgac cattatcaac agaacactcc   4920

aatcggcgac ggccctgtgc tcctcccaga caaccattac ctgtccaccc agtctgccct   4980

gtctaaagat cccaacgaaa agagagacca catggtcctg ctggagtttg tgaccgctgc   5040

tgggatcaca catggcatgg acgagctgta caagtaaaaa ttttgactag cggaggctag   5100

aaggagagag tagagctgga ttgtgcggac aaacgccgct tcacgcacct cgcgtgaccg   5160

gccagtactc ttaccacttt ccgcctactt ggtcgtcagc gctgttttgg gcactcctgt   5220

tgggggctgt tcgacgctcc acggtctcct ccgtactgac atctacggtg ttggggccgc   5280

cacgtgcgag ccgctcgcct ggtgtgcttc gactgtcacc cgacgcccgc ctttcacccc   5340

ccccccccta ggttctaccg tcgttcccga cgtttgaagg gaggaaacca cacgcttgca   5400

acaccactcc cggtgtcaac gggatgcaac cgcaagatag ccgttttcgc ccggaagtaa   5460

aacggcgggc ggccgcttcc ctttagtgag ggttaatgct tcgagcagac atgataagat   5520

acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg   5580

aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca   5640

acaacaattg cattcatttt atgtttcagg ttcaggggga gatgtgggag gttttttaaa   5700

gcaagtaaaa cctctacaaa tgtggtaaaa tccgataagg atcgatccgg gct          5753


<210>  8
<211>  10925
<212>  DNA
<213>  artificial

<220>
<223>  plasmid pDKIS-0107 that is used for expression in BHK21 cell 
       culture of the polyprotein of non-structural (NS) proteins of 
       FMDV fused to GFP: <2B-2C-3A-3B1/3B2/3B3-3C-3D-GFP>

<400>  8
tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta     60

ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc    120

aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg    180

gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc    240

gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat    300

agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc    360

ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga    420

cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg    480

gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac    540

caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt    600

caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg    660

cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata    720

agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac    780

agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt    840

gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa    900

ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact    960

cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac   1020

aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact   1080

ataggctagc ctcgagaatt cacgcgtggt acctctagag tcgaccctca cgccaacgac   1140

gactcccgcg tcgcactcca cacttacgtc tctgcgagtg taggaaccga cggactgtcg   1200

ctcaaagctt gcatgcctgc aggtcgactc tagaggatcc ccgtggcgcc cgaacaggga   1260

cctgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc   1320

gcgcacggca agaggcgagg ggcggcgact ggtgagatgt tcttcttctc cgacgtcagg   1380

gcgaacttca cgaagttggt ggacactgtc aaccagatgc aggaggacat gtcaacgaaa   1440

cacggacccg acttcaaccg actggtgtcc gcgtttgagg aattggccgc tggggtcaag   1500

gccatcagga ccggtctcga cgaggccaag ccctggtaca agctcatcaa acttctaagc   1560

cgcctgtcgt gcatggccgc tgtggcagca cggtccaagg acccagtcct tgtggccatc   1620

atgctggccg acaccggcct cgagattctg gacagcacct tcgtggtaaa gaagatctcc   1680

gactcgctct ccagtctctt ccacgtgccg gctcccgcct tcagtttcgg agccccgatc   1740

ctgttggccg ggttggtcaa agtcgcctcg agtttcttcc agtccacacc cgaagacctc   1800

gagagagcag aaaagcagct caaagcacgt gacatcaacg acatatttgc cgttcttaag   1860

aacggtgagt ggctggtcaa actgatcctg gccatccgcg actggattaa ggcatggatc   1920

gcctcagaag agaagtttgt caccatgaca gacctggtgc ctggcatcct tgaaagacaa   1980

cgggatctca atgaccccgg caaatacaag gaggccaagg aatggctgga caacgcgcgt   2040

caagcgtgtt tgaagagcgg gaacgtgcac attgccaatc tgtgtaaggt ggtcgctccg   2100

gcgcccagca agtcgagacc cgaaccagtg gtcgtgtgcc ttcgcggcaa atccggccaa   2160

gggaaaagct ttctcgcgaa cgttctcgcg caggcaattt ccacacactt cactggtagg   2220

accgactcgg tctggtactg cccgcccgac cctgaccact ttgacggtta caatcagcag   2280

accgtcgtcg tgatggacga cttgggccaa aacccagacg gcaaagactt caagtacttt   2340

gcccaaatgg tatccaccac ggggttcatc ccgcctatgg cctcgctcga ggataagggt   2400

aaacccttca acagcaaggt cataatagct acaaccaacc tgtactcggg attcacccca   2460

aagaccatgg tgtgccccga tgcgcttaac cggaggtttc actttgacat cgacgtgagc   2520

gccaaagacg ggtacaagat caacaacaaa ctggacatag tcaaagcact tgaagacacc   2580

cacgctaacc cggtggcgat gttccaatac gactgcgctc ttctcaacgg aatggccgtt   2640

gaaatgaaga gaatgcagca agacatgttc aagcctcaac cacccctcca gaacatctac   2700

cagctcgttc aggaggtgat tgagcgggtg gaactacacg aaaaggtgtc gagccacctg   2760

atatttaaac agatttcaat cccttcccag aagtccgtgt tgtacttcct cattgagaag   2820

ggccagcacg aagcagcgat cgagttcttc gaggggatgg tccacgattc catcaaagag   2880

gaactccgac ccctcattca gcagacctcg ttcgtaaaac gcgccttcaa gcgcctgaaa   2940

gagaactttg aagttgtagc tctgtgtttg accctcttgg caaacatagt gattatgctc   3000

cgccaagcgc gcaagaggta ccaatcggtg gatgacccac tggacggcga cgtaactctt   3060

ggcgacgcgg aaaagaaccc tctggagacg agtggcgcta gcgctgtcgg tttccgagag   3120

agatccccca ccgagcaagg gacgcgcgaa gacgcgaacg ctgagcccgt cgtgttcggt   3180

agggaacaac cgcgagctga aggaccctac gctgggccac tcgagcgtca gaaacctctt   3240

aaagtgaaag ccgagctgcc acaacaggag ggaccatacg ccggcccaat ggagagacag   3300

aaaccgctaa aggtgaaagc aaaagccccc gtcgtgaagg aaggacctta cgagggaccg   3360

gtgaagaaac ctgtcgcttt gaaagtgaaa gcaaagaact tgatagtcac tgagagtggt   3420

gcgccaccga ccgacttgca aaagatggtc atgggcaaca ctaagccagt cgagctcatc   3480

ctcgacggca agacggtagc catttgctgt gctaccggag tgttcggcac tgcctacctc   3540

gtgcctcgtc atctcttcgc ggaaaagtac gacaagatca tgttggacgg cagagccttg   3600

acagacagtg actacagagt gtttgagttt gagattaaag taaaaggaca ggacatgctc   3660

tcagacgccg ctctcatggt gttgcaccgt gggaatcgcg tgcgtgacat cacgaaacac   3720

tttcgtgacg tagcgagaat gaagaaggga acccccgtcg tcggtgtgat caacaatgct   3780

gacgtcggga gactcatatt ctctggtgaa gcccttactt acaaggacat cgtcgtgtgt   3840

atggatggag acaccatgcc tgggctcttt gcctacaggg catccaccaa ggcaggctac   3900

tgtggaggag ccgtcctggc aaaggacggg gccgaaacgt tcatcgttgg cacccactcc   3960

gcaggtggaa acggcatagg atactgttcg tgtgtttccc gatcaatgct cctgaagatg   4020

aaggcacaca tcgaccctga accacaccac gaggggttga tcgtcgacac cagagatgtg   4080

gaggagcgtg tccacgtgat gcgcaaaacc aagctcgcgc ccaccgtggc gcacggtgtg   4140

ttcaaccctg agttcgggcc tgccgctctg tccaacaagg acccgcgcct gaacgaaggg   4200

gttgtccttg acgatgtcat tttctccaaa cacaaaggag atacaaggat gtctgaagag   4260

gacaaagcgc tgtttcggcg ctgtgctgct gactacgcgt cgcgtctaca cagtgtgttg   4320

gggacagcaa acgccccact gagtgtgtat gaagccatca aaggcgtcga cggacttgac   4380

gccatggagc cggacacggc gcccggtctc ccctgggctc tccaagggaa acgccgcggc   4440

gccctgatcg acttcgaaaa cggcaccgtc gggcctgagg ttgaggcagc actcaagctc   4500

atggaaagcc gcgagtacaa attcgtctgc caaaccttcc tgaaggacga aattcggccg   4560

ctagaaaagg tacgcgctgg caagacacgc attgtcgacg tgttgcctgt tgaacacatt   4620

ctctacacca gaatgatgat tggcagattc tgtgctcaga tgcattcaaa caacggaccg   4680

caaattggat cagcggtcgg ttgtaaccct gacgttgatt ggcaaagatt tggcacacat   4740

ttcgcccagt acaaaaacgt gtgggatgtg gactactcag cctttgatgc aaaccactgc   4800

agcgatgcga tgaacatcat gttcgaggaa gtgttccgca cggagttcgg attccacccg   4860

aacgccgagt ggattctgaa gactctagtg aacacggagc acgcttacga gaacaagcgc   4920

attgttgttg aaggtggaat gccgtccggt tgttccgcaa caagcatcat caacacaatt   4980

ttgaacaaca tctacgtgct ttacgccctg cgtaggcact atgagggagt cgagctggac   5040

acttacacca tgatctctta tggagacgac atcgtggtgg caagtgacta cgacctggac   5100

tttgaggctc tcaagcccca cttcaagtcc cttggtcaga ctatcactcc ggccgacaaa   5160

agcgacaaag gttttgttct tggtcactcc ataaccgacg tcactttcct caaaagacac   5220

ttccacatgg actacggaac tgggttttac aaacctgtga tggcctcgaa gaccctcgag   5280

gccatcctct cctttgcacg ccgtgggacc atacaggaga agttgatctc cgtggcagga   5340

ctcgccgtcc actccggacc tgatgaatac cggcgcctct ttgagccctt ccaaggcctc   5400

ttcgagattc caagctacag atcactttac ctgcgatggg tgaacgccgt gtgcggtgac   5460

gcacgtcgca aacgtagcaa gggcgaggaa ctgttcactg gcgtggtccc aattctcgtg   5520

gaactggatg gcgatgtgaa tgggcacaaa ttttctgtca gcggagaggg tgaaggtgat   5580

gccacatacg gaaagctcac cctgaaattc atctgcacca ctggaaagct ccctgtgcca   5640

tggccaacac tggtcactac cttcwcyyat ggcgtgcagt gcttttccag atacccagac   5700

catatgaagc agcatgactt tttcaagagc gccatgcccg agggctatgt gcaggagaga   5760

accatctttt tcaaagatga cgggaactac aagacccgcg ctgaagtcaa gttcgaaggt   5820

gacaccctgg tgaatagaat cgagttgaag ggcattgact ttaaggaaga tggaaacatt   5880

ctcggccaca agctggaata caactataac tcccacaatg tgtacatcat ggccgacaag   5940

caaaagaatg gcatcaaggt caacttcaag atcagacaca acattgagga tggatccgtg   6000

cagctggccg accattatca acagaacact ccaatcggcg acggccctgt gctcctccca   6060

gacaaccatt acctgtccac ccagtctgcc ctgtctaaag atcccaacga aaagagagac   6120

cacatggtcc tgctggagtt tgtgaccgct gctgggatca cacatggcat ggacgagctg   6180

tacaagtaaa aattttgact agcggaggct agaaggagag agtagagctg gattgtgcgg   6240

acaaacgccg cttcacgcac ctcgcgtgac cggccagtac tcttaccact ttccgcctac   6300

ttggtcgtca gcgctgtttt gggcactcct gttgggggct gttcgacgct ccacggtctc   6360

ctccgtactg acatctacgg tgttggggcc gccacgtgcg agccgctcgc ctggtgtgct   6420

tcgactgtca cccgacgccc gcctttcacc cccccccccc taggttctac cgtcgttccc   6480

gacgtttgaa gggaggaaac cacacgcttg caacaccact cccggtgtca acgggatgca   6540

accgcaagat agccgttttc gcccggaagt aaaacggcgg gcggccgctt ccctttagtg   6600

agggttaatg cttcgagcag acatgataag atacattgat gagtttggac aaaccacaac   6660

tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt   6720

aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca   6780

ggttcagggg gagatgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtaa   6840

aatccgataa ggatcgatcc gggctggcgt aatagcgaag aggcccgcac cgatcgccct   6900

tcccaacagt tgcgcagcct gaatggcgaa tggacgcgcc ctgtagcggc gcattaagcg   6960

cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg   7020

ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc   7080

taaatcgggg gctcccttta gggttccgat ttagagcttt acggcacctc gaccgcaaaa   7140

aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc   7200

ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac   7260

tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt   7320

ggttaaaaaa tgagctgatt taacaaatat ttaacgcgaa ttttaacaaa atattaacgt   7380

ttacaatttc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc   7440

atacgcggat ctgcgcagca ccatggcctg aaataacctc tgaaagagga acttggttag   7500

gtaccttctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt   7560

ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca   7620

ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt   7680

agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt   7740

ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg   7800

cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt   7860

gcaaaaagct tgattcttct gacacaacag tctcgaactt aaggctagag ccaccatgat   7920

tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta   7980

tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca   8040

ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga   8100

cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga   8160

cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct   8220

cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg   8280

gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga   8340

gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca   8400

tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga   8460

ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg   8520

cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc   8580

gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt   8640

gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga   8700

gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca   8760

tcacgatggc cgcaataaaa tatctttatt ttcattacat ctgtgtgttg gttttttgtg   8820

tgaatcgata gcgataagga tccgcgtatg gtgcactctc agtacaatct gctctgatgc   8880

cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg   8940

tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca   9000

gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt   9060

tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg   9120

aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct   9180

catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat   9240

tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc   9300

tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg   9360

ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg   9420

ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga   9480

cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta   9540

ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc   9600

tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc   9660

gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg   9720

ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc   9780

aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca   9840

acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct   9900

tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat   9960

cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg  10020

gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat  10080

taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact  10140

tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat  10200

cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc  10260

ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct  10320

accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg  10380

cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca  10440

cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc  10500

tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga  10560

taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac  10620

gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga  10680

agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag  10740

ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg  10800

acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag  10860

caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tggctcgaca  10920

gatct                                                              10925


<210>  9
<211>  2729
<212>  DNA
<213>  artificial

<220>
<223>  a sequence comprising (1) UTR containing HCV subtype 1b IRES 
       fused to (2) cDNA encoding the polyprotein of HCV subtype 1b 
       structural proteins <C-E1-E2-p7>

<400>  9
ctcccctgtg aggaactact gtcttcacgc agaaagcgtc tagccatggc gttagtatga     60

gtgtcgtgca gcctccagga ccccccctcc cgggagagcc atagtggtct gcggaaccgg    120

tgagtacacc ggaattgcca ggacgaccgg gtcctttctt ggatcaaccc gctcaatgcc    180

tggagatttg ggcgtgcccc cgcgagactg ctagccgagt agtgttgggt cgcgaaaggc    240

cttgtggtac tgcctgatag ggtgcttgcg agtgccccgg gaggtctcgt agaccgtgca    300

ccatgagcac gaatcctaaa cctcaaagaa aaaccaaacg taacaccaac cgccgcccac    360

aggacgtcaa gttcccgggc ggtggtcaga tcgttggcgg agtttacctg ttgccgcgca    420

ggggccccag gttgggcgtg cgcgcgacta ggaagacttc cgagcggtcg caacctcgtg    480

gaaggcgaca acctatcccc aaggctcgcc atcccgaggg caggacctgg gctcagcctg    540

ggtacccttg gcccctctat ggcaatgagg gcttggggtg ggcaggatgg ctcctgtcac    600

cccgtggctc tcggcctagt tggggcccca cggacccccg gcgtaggtcg cgcaatttgg    660

gtaaggtcat cgataccctc acgtgcggct tcgccgacct catggggtac attccgctcg    720

tcggcgcccc cctagggggc gtcgccaggg ccctggcgca tggcgtccgg gtcctggagg    780

acggcgtgaa ttacgcaaca gggaatctgc ccggttgctc tttctctatc ttcctcttgg    840

ctttattgtc ctgcctgacc atcccagctt ccgcttatga agtgcgcaac gtgtccgggg    900

tgtaccatgt cacgaacgac tgctccaact caagcatagt gtatgaggca gcggacgtga    960

tcatgcatac tcccgggtgc gtgccctgcg tgcaggatgg caacacctcc cgctgttggg   1020

tagcgctcac tcccacgctt gcggccagga atgccagcgt ccccgtcaca gcaatacgac   1080

gtcatgtcga tttgctcgtt gggacggctg ctttctgttc cgctatgtac gtgggagatc   1140

tttgcggatc tgtcttcccc gtctcccagc tgttcacctt ttcgcctcgc cggcatcaga   1200

cagtacagga ctgcaactgt tcaatttatc ccggccatat atcaggccac cgcatggcct   1260

gggatatgat gatgaactgg tcacctacgg cagccctagt ggtatcgcag ttactccgga   1320

tcccacaagc catcgtggat atggtggcgg gggcccactg gggagtcctg gcgggccttg   1380

cctactattc catggtgggg aactgggcta aggttatgat tgttttgcta ctctttgccg   1440

gcgttgacgg gactacccac accacagggg gggcggcagc ccgcgctacc cagggattca   1500

catctttctt ctcacttggg ccgtctcaga aaatccagct cataaatacc aacggcagct   1560

ggcacatcaa cagaactgcc ctgaactgca atgactccct ccagactggg ttccttgccg   1620

cgctgttcta cacatacagg ttcaacgcgt ccggatgccc agagcgcatg gccagctgcc   1680

gccccattga caagttcgat caagggtggg gtcccatcac ttatgctgag cctgacagct   1740

cggaccagag gccctactgc tggcactacg cgcctagacc gtgtggtatt gtacccgcat   1800

cgcaggtgtg tggtccagtg tactgcttca ccccaagccc ggttgtagtg gggacaaccg   1860

accgtttcgg tgtccctacg tatacctggg gggagaatga gacggacgtg ctgctcctca   1920

acaacacgcg gccgccgctg ggcaactggt tcggctgtac atggatgaac agcactgggt   1980

tcaccaagac gtgcgggggc cccccgtgta acatcggggg ggccggtaac accaccttga   2040

cctgccccac ggattgcttc cggaaacacc ccgaggccac ttacaccaaa tgcggctcgg   2100

ggccttggtt gacacctagg tgcttggttg attatccata caggctctgg cattacccct   2160

gcgctgtcaa ctttaccatc ttcaaggtta ggatgtatgt ggggggcgtg gagcataggc   2220

tcaacgctgc atgcaattgg actcggggag agcgctgcga cctagaggac agggataggt   2280

cagagctcag cccgctgcta ctgtctacaa cagagtggca ggtactgccc tgttccttca   2340

ccaccctgcc ggccctgtcc actggtttga ttcacctcca tcagaacatc gtggacgtgc   2400

agtacctgta cggtataggg tcagcggtca tcccctttgc aatcaaatgg gagtatgtcc   2460

tgctgctctt ccttctcctg gcggacgcgc gcgtctgcgc ctgcttgtgg atgatgctgc   2520

tgatagctca agctgaggcc gccttggaga acttggtggt cctcaatgct gcgtccgtgg   2580

ccggagcgca tggcattctc tccttccttg tgttcttctg tgctgcctgg tacatcaagg   2640

gcaggctggt ccccggggcg gcatatgctc tttatggcgt atggccgctg ctcttgctcc   2700

tgctggcatt accaccacga gcatatgcc                                     2729


<210>  10
<211>  4340
<212>  DNA
<213>  artificial

<220>
<223>  "VLP-producing" construct designed for expression in BHK21 cell 
       culture of polyprotein of structural proteins of HCV type 1b 
       <C-E1-E2-p7> under the control of FMDV replication machinery

<400>  10
tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc     60

ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat    120

gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt    180

acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat    240

ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt    300

cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa    360

actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc    420

aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct    480

acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag    540

tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt    600

gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac    660

aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc    720

agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact    780

cactataggg agacccaagc tggctagcgt ttaaacttaa gcttggtacc gagctcggat    840

ccactagtcc agtgtggtgg aatttcacgc caacgacgac tcccgcgtcg cactccacac    900

ttacgtctct gcgagtgtag gaaccgacgg actgtcgctc aaagcttgca tgcctgcagg    960

tcgactctag aggatcccct cccctgtgag gaactactgt cttcacgcag aaagcgtcta   1020

gccatggcgt tagtatgagt gtcgtgcagc ctccaggacc ccccctcccg ggagagccat   1080

agtggtctgc ggaaccggtg agtacaccgg aattgccagg acgaccgggt cctttcttgg   1140

atcaacccgc tcaatgcctg gagatttggg cgtgcccccg cgagactgct agccgagtag   1200

tgttgggtcg cgaaaggcct tgtggtactg cctgataggg tgcttgcgag tgccccggga   1260

ggtctcgtag accgtgcacc atgagcacga atcctaaacc tcaaagaaaa accaaacgta   1320

acaccaaccg ccgcccacag gacgtcaagt tcccgggcgg tggtcagatc gttggcggag   1380

tttacctgtt gccgcgcagg ggccccaggt tgggcgtgcg cgcgactagg aagacttccg   1440

agcggtcgca acctcgtgga aggcgacaac ctatccccaa ggctcgccat cccgagggca   1500

ggacctgggc tcagcctggg tacccttggc ccctctatgg caatgagggc ttggggtggg   1560

caggatggct cctgtcaccc cgtggctctc ggcctagttg gggccccacg gacccccggc   1620

gtaggtcgcg caatttgggt aaggtcatcg ataccctcac gtgcggcttc gccgacctca   1680

tggggtacat tccgctcgtc ggcgcccccc tagggggcgt cgccagggcc ctggcgcatg   1740

gcgtccgggt cctggaggac ggcgtgaatt acgcaacagg gaatctgccc ggttgctctt   1800

tctctatctt cctcttggct ttattgtcct gcctgaccat cccagcttcc gcttatgaag   1860

tgcgcaacgt gtccggggtg taccatgtca cgaacgactg ctccaactca agcatagtgt   1920

atgaggcagc ggacgtgatc atgcatactc ccgggtgcgt gccctgcgtg caggatggca   1980

acacctcccg ctgttgggta gcgctcactc ccacgcttgc ggccaggaat gccagcgtcc   2040

ccgtcacagc aatacgacgt catgtcgatt tgctcgttgg gacggctgct ttctgttccg   2100

ctatgtacgt gggagatctt tgcggatctg tcttccccgt ctcccagctg ttcacctttt   2160

cgcctcgccg gcatcagaca gtacaggact gcaactgttc aatttatccc ggccatatat   2220

caggccaccg catggcctgg gatatgatga tgaactggtc acctacggca gccctagtgg   2280

tatcgcagtt actccggatc ccacaagcca tcgtggatat ggtggcgggg gcccactggg   2340

gagtcctggc gggccttgcc tactattcca tggtggggaa ctgggctaag gttatgattg   2400

ttttgctact ctttgccggc gttgacggga ctacccacac cacagggggg gcggcagccc   2460

gcgctaccca gggattcaca tctttcttct cacttgggcc gtctcagaaa atccagctca   2520

taaataccaa cggcagctgg cacatcaaca gaactgccct gaactgcaat gactccctcc   2580

agactgggtt ccttgccgcg ctgttctaca catacaggtt caacgcgtcc ggatgcccag   2640

agcgcatggc cagctgccgc cccattgaca agttcgatca agggtggggt cccatcactt   2700

atgctgagcc tgacagctcg gaccagaggc cctactgctg gcactacgcg cctagaccgt   2760

gtggtattgt acccgcatcg caggtgtgtg gtccagtgta ctgcttcacc ccaagcccgg   2820

ttgtagtggg gacaaccgac cgtttcggtg tccctacgta tacctggggg gagaatgaga   2880

cggacgtgct gctcctcaac aacacgcggc cgccgctggg caactggttc ggctgtacat   2940

ggatgaacag cactgggttc accaagacgt gcgggggccc cccgtgtaac atcggggggg   3000

ccggtaacac caccttgacc tgccccacgg attgcttccg gaaacacccc gaggccactt   3060

acaccaaatg cggctcgggg ccttggttga cacctaggtg cttggttgat tatccataca   3120

ggctctggca ttacccctgc gctgtcaact ttaccatctt caaggttagg atgtatgtgg   3180

ggggcgtgga gcataggctc aacgctgcat gcaattggac tcggggagag cgctgcgacc   3240

tagaggacag ggataggtca gagctcagcc cgctgctact gtctacaaca gagtggcagg   3300

tactgccctg ttccttcacc accctgccgg ccctgtccac tggtttgatt cacctccatc   3360

agaacatcgt ggacgtgcag tacctgtacg gtatagggtc agcggtcatc ccctttgcaa   3420

tcaaatggga gtatgtcctg ctgctcttcc ttctcctggc ggacgcgcgc gtctgcgcct   3480

gcttgtggat gatgctgctg atagctcaag ctgaggccgc cttggagaac ttggtggtcc   3540

tcaatgctgc gtccgtggcc ggagcgcatg gcattctctc cttccttgtg ttcttctgtg   3600

ctgcctggta catcaagggc aggctggtcc ccggggcggc atatgctctt tatggcgtat   3660

ggccgctgct cttgctcctg ctggcattac caccacgagc atatgcccgt cgcaaacgta   3720

ccatgattac actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc   3780

aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc   3840

gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt   3900

attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa   3960

tctgctctga tgccgcatag agctggattg tgcggacaaa cgccgcttca cgcacctcgc   4020

gtgaccggcc agtactctta ccactttccg cctacttggt cgtcagcgct gttttgggca   4080

ctcctgttgg gggctgttcg acgctccacg gtctcctccg tactgacatc tacggtgttg   4140

gggccgccac gtgcgagccg ctcgcctggt gtgcttcgac tgtcacccga cgcccgcctt   4200

tcaccccccc ccccctaggt tctaccgtcg ttcccgacgt ttgaagggag gaaaccacac   4260

gcttgcaaca ccactcccgg tgtcaacggg atgcaaccgc aagatagccg ttttcgcccg   4320

gaagtaaaac ggcaattctg                                               4340


<210>  11
<211>  3545
<212>  DNA
<213>  artificial

<220>
<223>  "ISVAC-FUN": fragment of pISVAC-0905 comprising FMDV-3'-UTR, 
       fusion HCV IRES-polyprotein, and FMDV-5'-UTR

<400>  11
tttaaactta agcttggtac cgagctcgga tccactagtc cagtgtggtg gaatttcacg     60

ccaacgacga ctcccgcgtc gcactccaca cttacgtctc tgcgagtgta ggaaccgacg    120

gactgtcgct caaagcttgc atgcctgcag gtcgactcta gaggatcccc tcccctgtga    180

ggaactactg tcttcacgca gaaagcgtct agccatggcg ttagtatgag tgtcgtgcag    240

cctccaggac cccccctccc gggagagcca tagtggtctg cggaaccggt gagtacaccg    300

gaattgccag gacgaccggg tcctttcttg gatcaacccg ctcaatgcct ggagatttgg    360

gcgtgccccc gcgagactgc tagccgagta gtgttgggtc gcgaaaggcc ttgtggtact    420

gcctgatagg gtgcttgcga gtgccccggg aggtctcgta gaccgtgcac catgagcacg    480

aatcctaaac ctcaaagaaa aaccaaacgt aacaccaacc gccgcccaca ggacgtcaag    540

ttcccgggcg gtggtcagat cgttggcgga gtttacctgt tgccgcgcag gggccccagg    600

ttgggcgtgc gcgcgactag gaagacttcc gagcggtcgc aacctcgtgg aaggcgacaa    660

cctatcccca aggctcgcca tcccgagggc aggacctggg ctcagcctgg gtacccttgg    720

cccctctatg gcaatgaggg cttggggtgg gcaggatggc tcctgtcacc ccgtggctct    780

cggcctagtt ggggccccac ggacccccgg cgtaggtcgc gcaatttggg taaggtcatc    840

gataccctca cgtgcggctt cgccgacctc atggggtaca ttccgctcgt cggcgccccc    900

ctagggggcg tcgccagggc cctggcgcat ggcgtccggg tcctggagga cggcgtgaat    960

tacgcaacag ggaatctgcc cggttgctct ttctctatct tcctcttggc tttattgtcc   1020

tgcctgacca tcccagcttc cgcttatgaa gtgcgcaacg tgtccggggt gtaccatgtc   1080

acgaacgact gctccaactc aagcatagtg tatgaggcag cggacgtgat catgcatact   1140

cccgggtgcg tgccctgcgt gcaggatggc aacacctccc gctgttgggt agcgctcact   1200

cccacgcttg cggccaggaa tgccagcgtc cccgtcacag caatacgacg tcatgtcgat   1260

ttgctcgttg ggacggctgc tttctgttcc gctatgtacg tgggagatct ttgcggatct   1320

gtcttccccg tctcccagct gttcaccttt tcgcctcgcc ggcatcagac agtacaggac   1380

tgcaactgtt caatttatcc cggccatata tcaggccacc gcatggcctg ggatatgatg   1440

atgaactggt cacctacggc agccctagtg gtatcgcagt tactccggat cccacaagcc   1500

atcgtggata tggtggcggg ggcccactgg ggagtcctgg cgggccttgc ctactattcc   1560

atggtgggga actgggctaa ggttatgatt gttttgctac tctttgccgg cgttgacggg   1620

actacccaca ccacaggggg ggcggcagcc cgcgctaccc agggattcac atctttcttc   1680

tcacttgggc cgtctcagaa aatccagctc ataaatacca acggcagctg gcacatcaac   1740

agaactgccc tgaactgcaa tgactccctc cagactgggt tccttgccgc gctgttctac   1800

acatacaggt tcaacgcgtc cggatgccca gagcgcatgg ccagctgccg ccccattgac   1860

aagttcgatc aagggtgggg tcccatcact tatgctgagc ctgacagctc ggaccagagg   1920

ccctactgct ggcactacgc gcctagaccg tgtggtattg tacccgcatc gcaggtgtgt   1980

ggtccagtgt actgcttcac cccaagcccg gttgtagtgg ggacaaccga ccgtttcggt   2040

gtccctacgt atacctgggg ggagaatgag acggacgtgc tgctcctcaa caacacgcgg   2100

ccgccgctgg gcaactggtt cggctgtaca tggatgaaca gcactgggtt caccaagacg   2160

tgcgggggcc ccccgtgtaa catcgggggg gccggtaaca ccaccttgac ctgccccacg   2220

gattgcttcc ggaaacaccc cgaggccact tacaccaaat gcggctcggg gccttggttg   2280

acacctaggt gcttggttga ttatccatac aggctctggc attacccctg cgctgtcaac   2340

tttaccatct tcaaggttag gatgtatgtg gggggcgtgg agcataggct caacgctgca   2400

tgcaattgga ctcggggaga gcgctgcgac ctagaggaca gggataggtc agagctcagc   2460

ccgctgctac tgtctacaac agagtggcag gtactgccct gttccttcac caccctgccg   2520

gccctgtcca ctggtttgat tcacctccat cagaacatcg tggacgtgca gtacctgtac   2580

ggtatagggt cagcggtcat cccctttgca atcaaatggg agtatgtcct gctgctcttc   2640

cttctcctgg cggacgcgcg cgtctgcgcc tgcttgtgga tgatgctgct gatagctcaa   2700

gctgaggccg ccttggagaa cttggtggtc ctcaatgctg cgtccgtggc cggagcgcat   2760

ggcattctct ccttccttgt gttcttctgt gctgcctggt acatcaaggg caggctggtc   2820

cccggggcgg catatgctct ttatggcgta tggccgctgc tcttgctcct gctggcatta   2880

ccaccacgag catatgcccg tcgcaaacgt accatgatta cactggccgt cgttttacaa   2940

cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct   3000

ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc   3060

agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt   3120

tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata gagctggatt   3180

gtgcggacaa acgccgcttc acgcacctcg cgtgaccggc cagtactctt accactttcc   3240

gcctacttgg tcgtcagcgc tgttttgggc actcctgttg ggggctgttc gacgctccac   3300

ggtctcctcc gtactgacat ctacggtgtt ggggccgcca cgtgcgagcc gctcgcctgg   3360

tgtgcttcga ctgtcacccg acgcccgcct ttcacccccc cccccctagg ttctaccgtc   3420

gttcccgacg tttgaaggga ggaaaccaca cgcttgcaac accactcccg gtgtcaacgg   3480

gatgcaaccg caagatagcc gttttcgccc ggaagtaaaa cggcaattct gcagatatcc   3540

agcac                                                               3545


<210>  12
<211>  8901
<212>  DNA
<213>  artificial

<220>
<223>  plasmid pISVAC-0905 that is used for HCV subtype 1b VLP ISVAC 
       production in BHK21 cell culture and encodes polyprotein of 
       structural proteins of HCV type 1b fused to beta-galactosidase 
       alpha-peptide <C-E1-E2-p7-beta-gal(alpha)pep>

<400>  12
gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg     60

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg    120

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc    180

ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt    240

gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata    300

tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc    360

cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc    420

attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt    480

atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt    540

atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca    600

tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg    660

actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc    720

aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg    780

gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca    840

ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc    900

gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaatttcac    960

gccaacgacg actcccgcgt cgcactccac acttacgtct ctgcgagtgt aggaaccgac   1020

ggactgtcgc tcaaagcttg catgcctgca ggtcgactct agaggatccc ctcccctgtg   1080

aggaactact gtcttcacgc agaaagcgtc tagccatggc gttagtatga gtgtcgtgca   1140

gcctccagga ccccccctcc cgggagagcc atagtggtct gcggaaccgg tgagtacacc   1200

ggaattgcca ggacgaccgg gtcctttctt ggatcaaccc gctcaatgcc tggagatttg   1260

ggcgtgcccc cgcgagactg ctagccgagt agtgttgggt cgcgaaaggc cttgtggtac   1320

tgcctgatag ggtgcttgcg agtgccccgg gaggtctcgt agaccgtgca ccatgagcac   1380

gaatcctaaa cctcaaagaa aaaccaaacg taacaccaac cgccgcccac aggacgtcaa   1440

gttcccgggc ggtggtcaga tcgttggcgg agtttacctg ttgccgcgca ggggccccag   1500

gttgggcgtg cgcgcgacta ggaagacttc cgagcggtcg caacctcgtg gaaggcgaca   1560

acctatcccc aaggctcgcc atcccgaggg caggacctgg gctcagcctg ggtacccttg   1620

gcccctctat ggcaatgagg gcttggggtg ggcaggatgg ctcctgtcac cccgtggctc   1680

tcggcctagt tggggcccca cggacccccg gcgtaggtcg cgcaatttgg gtaaggtcat   1740

cgataccctc acgtgcggct tcgccgacct catggggtac attccgctcg tcggcgcccc   1800

cctagggggc gtcgccaggg ccctggcgca tggcgtccgg gtcctggagg acggcgtgaa   1860

ttacgcaaca gggaatctgc ccggttgctc tttctctatc ttcctcttgg ctttattgtc   1920

ctgcctgacc atcccagctt ccgcttatga agtgcgcaac gtgtccgggg tgtaccatgt   1980

cacgaacgac tgctccaact caagcatagt gtatgaggca gcggacgtga tcatgcatac   2040

tcccgggtgc gtgccctgcg tgcaggatgg caacacctcc cgctgttggg tagcgctcac   2100

tcccacgctt gcggccagga atgccagcgt ccccgtcaca gcaatacgac gtcatgtcga   2160

tttgctcgtt gggacggctg ctttctgttc cgctatgtac gtgggagatc tttgcggatc   2220

tgtcttcccc gtctcccagc tgttcacctt ttcgcctcgc cggcatcaga cagtacagga   2280

ctgcaactgt tcaatttatc ccggccatat atcaggccac cgcatggcct gggatatgat   2340

gatgaactgg tcacctacgg cagccctagt ggtatcgcag ttactccgga tcccacaagc   2400

catcgtggat atggtggcgg gggcccactg gggagtcctg gcgggccttg cctactattc   2460

catggtgggg aactgggcta aggttatgat tgttttgcta ctctttgccg gcgttgacgg   2520

gactacccac accacagggg gggcggcagc ccgcgctacc cagggattca catctttctt   2580

ctcacttggg ccgtctcaga aaatccagct cataaatacc aacggcagct ggcacatcaa   2640

cagaactgcc ctgaactgca atgactccct ccagactggg ttccttgccg cgctgttcta   2700

cacatacagg ttcaacgcgt ccggatgccc agagcgcatg gccagctgcc gccccattga   2760

caagttcgat caagggtggg gtcccatcac ttatgctgag cctgacagct cggaccagag   2820

gccctactgc tggcactacg cgcctagacc gtgtggtatt gtacccgcat cgcaggtgtg   2880

tggtccagtg tactgcttca ccccaagccc ggttgtagtg gggacaaccg accgtttcgg   2940

tgtccctacg tatacctggg gggagaatga gacggacgtg ctgctcctca acaacacgcg   3000

gccgccgctg ggcaactggt tcggctgtac atggatgaac agcactgggt tcaccaagac   3060

gtgcgggggc cccccgtgta acatcggggg ggccggtaac accaccttga cctgccccac   3120

ggattgcttc cggaaacacc ccgaggccac ttacaccaaa tgcggctcgg ggccttggtt   3180

gacacctagg tgcttggttg attatccata caggctctgg cattacccct gcgctgtcaa   3240

ctttaccatc ttcaaggtta ggatgtatgt ggggggcgtg gagcataggc tcaacgctgc   3300

atgcaattgg actcggggag agcgctgcga cctagaggac agggataggt cagagctcag   3360

cccgctgcta ctgtctacaa cagagtggca ggtactgccc tgttccttca ccaccctgcc   3420

ggccctgtcc actggtttga ttcacctcca tcagaacatc gtggacgtgc agtacctgta   3480

cggtataggg tcagcggtca tcccctttgc aatcaaatgg gagtatgtcc tgctgctctt   3540

ccttctcctg gcggacgcgc gcgtctgcgc ctgcttgtgg atgatgctgc tgatagctca   3600

agctgaggcc gccttggaga acttggtggt cctcaatgct gcgtccgtgg ccggagcgca   3660

tggcattctc tccttccttg tgttcttctg tgctgcctgg tacatcaagg gcaggctggt   3720

ccccggggcg gcatatgctc tttatggcgt atggccgctg ctcttgctcc tgctggcatt   3780

accaccacga gcatatgccc gtcgcaaacg taccatgatt acactggccg tcgttttaca   3840

acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc   3900

tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg   3960

cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat   4020

ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agagctggat   4080

tgtgcggaca aacgccgctt cacgcacctc gcgtgaccgg ccagtactct taccactttc   4140

cgcctacttg gtcgtcagcg ctgttttggg cactcctgtt gggggctgtt cgacgctcca   4200

cggtctcctc cgtactgaca tctacggtgt tggggccgcc acgtgcgagc cgctcgcctg   4260

gtgtgcttcg actgtcaccc gacgcccgcc tttcaccccc ccccccctag gttctaccgt   4320

cgttcccgac gtttgaaggg aggaaaccac acgcttgcaa caccactccc ggtgtcaacg   4380

ggatgcaacc gcaagatagc cgttttcgcc cggaagtaaa acggcaattc tgcagatatc   4440

cagcacagtg gcggccgctc gagtctagag ggcccgttta aacccgctga tcagcctcga   4500

ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc   4560

tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc   4620

tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt   4680

gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa   4740

gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg   4800

cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc   4860

ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa   4920

atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac   4980

ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt   5040

tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca   5100

accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt   5160

taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca   5220

gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct   5280

caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca   5340

aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc   5400

cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta   5460

tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt   5520

tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga   5580

tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc   5640

tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg   5700

ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac   5760

cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc   5820

cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg   5880

gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga   5940

gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg   6000

cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg   6060

tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt   6120

cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc   6180

ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg   6240

gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga   6300

gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc   6360

gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc   6420

gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc   6480

ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag   6540

cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat   6600

ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat   6660

tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc   6720

tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg   6780

ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa   6840

tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac   6900

ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt   6960

gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga   7020

gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca   7080

ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg   7140

ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt   7200

cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc   7260

ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct   7320

tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc   7380

gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta   7440

tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca   7500

gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag   7560

tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag   7620

ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt   7680

agcggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat   7740

cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt   7800

ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt   7860

tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc   7920

agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc   7980

gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata   8040

ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg   8100

gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc   8160

cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct   8220

acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa   8280

cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt   8340

cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca   8400

ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac   8460

tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca   8520

atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt   8580

tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc   8640

actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca   8700

aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata   8760

ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc   8820

ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc   8880

cgaaaagtgc cacctgacgt c                                             8901


<210>  13
<211>  98
<212>  DNA
<213>  Foot-and-mouth disease virus

<400>  13
taaccacaag catactgccg ccttccccgg cgttaacggg atgcaaccgt aagacacacc     60

ttcatccgga agtaaaacgg ctgcgtcaca tagttttg                             98


<210>  14
<211>  11
<212>  PRT
<213>  Human immunodeficiency virus type 1

<400>  14

Tyr Gly Arg Lys Lys Arg Arg Gln Arg Arg Arg 
1               5                   10      


