                         SEQUENCE LISTING

<110>  University of Southampton
       Roach, Peter Lee
       Wood, Robert James
       Maynard-Smith,Michael David

<120>  Partially-methylated break light assay for DNA methyltransferase 
       activity

<130>  P54779WO

<160>  13    

<170>  PatentIn version 3.3

<210>  1
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide

<400>  1
ccggatccag ttttctggat ccgg                                            24


<210>  2
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide


<220>
<221>  modified_base
<222>  (4)..(4)
<223>  m6a

<400>  2
ccggntccag ttttctggat ccgg                                            24


<210>  3
<211>  16
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(16)
<223>  complementary sequences

<400>  3
ngcgcntttt ngcgcn                                                     16


<210>  4
<211>  16
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(16)
<223>  complementary sequences

<220>
<221>  modified_base
<222>  (5)..(5)
<223>  m5c

<220>
<221>  modified_base
<222>  (13)..(13)
<223>  m5c

<220>
<221>  modified_base
<222>  (15)..(15)
<223>  m5c

<400>  4
ngcgnntttt ngngnn                                                     16


<210>  5
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(18)
<223>  complementary sequences

<400>  5
ngcngcnttt tngcngcn                                                   18


<210>  6
<211>  59
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  6
ggcggcccat gggccaccac caccaccacc acaagaaaaa ccgcgctttt ttaaaatgg      59


<210>  7
<211>  33
<212>  DNA
<213>  Primer

<400>  7
gccgccaagc tttcagctat agagcgccaa aag                                  33


<210>  8
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  C modified with fluorescein

<220>
<221>  modified_base
<222>  (5)..(5)
<223>  m6a

<220>
<221>  misc_feature
<222>  (24)..(24)
<223>  G modified with dabcyl quencher


<400>  8
ncggntccag ttttctggat ccgn                                            24


<210>  9
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  C modified with fluorescein

<220>
<221>  modified_base
<222>  (5)..(5)
<223>  m6a


<220>
<221>  modified_base
<222>  (19)..(19)
<223>  m6a

<220>
<221>  misc_feature
<222>  (24)..(24)
<223>  G modified with dabcyl quencher

<400>  9
ncggntccag ttttctggnt ccgn                                            24


<210>  10
<211>  32
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  C modified with fluor(Cy3) 

<220>
<221>  modified_base
<222>  (9)..(27)
<223>  m6a

<220>
<221>  misc_feature
<222>  (32)..(32)
<223>  G modified with dabcyl quencher

<400>  10
nctatgcgna tcagttttct gatgngnata gn                                   32


<210>  11
<211>  32
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  C modified with fluor (Cy3)

<220>
<221>  modified_base
<222>  (7)..(27)
<223>  m6a

<220>
<221>  misc_feature
<222>  (32)..(32)
<223>  G modified with dabcyl quencher

<400>  11
nctatgngna tcagttttct gatgngnata gn                                   32


<210>  12
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  G modified with fluor(Cy3)

<220>
<221>  modified_base
<222>  (7)..(21)
<223>  m6a

<220>
<221>  misc_feature
<222>  (24)..(24)
<223>  C modified with dabcyl quencher

<400>  12
natgcgnatc ttttgatgng natn                                            24


<210>  13
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  G modified with fluor (Cy3)

<220>
<221>  modified_base
<222>  (5)..(21)
<223>  m6a

<220>
<221>  misc_feature
<222>  (24)..(24)
<223>  C modified with dabcyl quencher

<400>  13
natgngnatc ttttgatgng natn                                            24


