                                SEQUENCE LISTING

<110> Grace DeSantis

<120> NITRILASES, NUCLEIC ACIDS ENCODING THEM 
      AND METHODS FOR MAKING AND USING THEM

<130> D1440-15-WO

<140> Unknown

<141> 2008-02-29

<160> 32

<170> PatentIn 3.4

<210> 1
<211> 900
<212> DNA
<213> Unknown

<220> 
<223> Obtained from environmental sample

<400> 1
atgacgacaa gaatcggcgt ggtacaggcg ggttcaatcc tcggcgacac accgcgaacg     60

ctcgacaagc ttgcgcaact ctgtgcagcg tgcgctcagc gacgtctgca ccttgccgtc    120

tttcctgaag cgttcattgg cggctacccg aaggggctgg tatttggcgc ctcggtgggc    180

attcgcaccg cggccggccg cgacctgttc cgacgctatg ccgactgtgc catcgacgtc    240

ccgggtccgg taacggcggt gatcggcggg ttggccaaag aagcagccct gcatcttgtc    300

atcggcgtga tcgaacgcga cgggggaacg ctgtattgct cggcgctcta tttcgccccg    360

gacggcaagc ttctcggcaa acatcgcaag ctgattccta cggcagcgga gcgcttgatc    420

tggggctgcg gagacggctc gacgttaagc gttttcgatg cacccatcgg aaagatcggc    480

ggattgatct gctgggaaaa ctacatgccg ctggcgcgcc tgtcgatgta cgcacaagga    540

gtggaactct actgcgctcc gaccgtggat gatcgggaag tttggattcc gaccgtcaga    600

cacatcgcgc gcgagggccg ctgtttcgta ctgagcagct gccagttcct ctctcgaagc    660

gcgtattccg ccgattggct cgccaccgcc gagaacctcc cggacgtgcc catccgcggc    720

ggcagctgca tcatcgggcc gatgggggaa tttctggcgg aaccggcata tggcgaggag    780

acgatcctct cggcggacat cgacctcgcc gatttgacgc gcgccaagtt cgacttcgat    840

gtggtcggcc attatgcgcg gccggacgtc ttcgagtttt cggtgaagcc gggcgcctaa    900

<210> 2
<211> 299
<212> PRT
<213> Unknown

<220> 
<223> Obtained from environmental sample

<220> 
<221> DOMAIN
<222> (4)...(189)
<223> Carbon-nitrogen hydrolase

<220> 
<221> SITE
<222> (162)...(175)
<223> Nitrilases / cyanide hydratase active site signature. Prosite id = PS00921

<400> 2
Met Thr Thr Arg Ile Gly Val Val Gln Ala Gly Ser Ile Leu Gly Asp 
1               5                   10                  15      


Thr Pro Arg Thr Leu Asp Lys Leu Ala Gln Leu Cys Ala Ala Cys Ala 
            20                  25                  30          


Gln Arg Arg Leu His Leu Ala Val Phe Pro Glu Ala Phe Ile Gly Gly 
        35                  40                  45              


Tyr Pro Lys Gly Leu Val Phe Gly Ala Ser Val Gly Ile Arg Thr Ala 
    50                  55                  60                  


Ala Gly Arg Asp Leu Phe Arg Arg Tyr Ala Asp Cys Ala Ile Asp Val 
65                  70                  75                  80  


Pro Gly Pro Val Thr Ala Val Ile Gly Gly Leu Ala Lys Glu Ala Ala 
                85                  90                  95      


Leu His Leu Val Ile Gly Val Ile Glu Arg Asp Gly Gly Thr Leu Tyr 
            100                 105                 110         


Cys Ser Ala Leu Tyr Phe Ala Pro Asp Gly Lys Leu Leu Gly Lys His 
        115                 120                 125             


Arg Lys Leu Ile Pro Thr Ala Ala Glu Arg Leu Ile Trp Gly Cys Gly 
    130                 135                 140                 


Asp Gly Ser Thr Leu Ser Val Phe Asp Ala Pro Ile Gly Lys Ile Gly 
145                 150                 155                 160 


Gly Leu Ile Cys Trp Glu Asn Tyr Met Pro Leu Ala Arg Leu Ser Met 
                165                 170                 175     


Tyr Ala Gln Gly Val Glu Leu Tyr Cys Ala Pro Thr Val Asp Asp Arg 
            180                 185                 190         


Glu Val Trp Ile Pro Thr Val Arg His Ile Ala Arg Glu Gly Arg Cys 
        195                 200                 205             


Phe Val Leu Ser Ser Cys Gln Phe Leu Ser Arg Ser Ala Tyr Ser Ala 
    210                 215                 220                 


Asp Trp Leu Ala Thr Ala Glu Asn Leu Pro Asp Val Pro Ile Arg Gly 
225                 230                 235                 240 


Gly Ser Cys Ile Ile Gly Pro Met Gly Glu Phe Leu Ala Glu Pro Ala 
                245                 250                 255     


Tyr Gly Glu Glu Thr Ile Leu Ser Ala Asp Ile Asp Leu Ala Asp Leu 
            260                 265                 270         


Thr Arg Ala Lys Phe Asp Phe Asp Val Val Gly His Tyr Ala Arg Pro 
        275                 280                 285             


Asp Val Phe Glu Phe Ser Val Lys Pro Gly Ala 
    290                 295                 


<210> 3
<211> 939
<212> DNA
<213> Unknown

<220> 
<223> Obtained from environmental sample

<400> 3
atgtcgacga aagttgccat cgtccaggcg ccgccggtcc tgctgcatcg cgacaggacg     60

attgcgaagg tgcgttcgtc gatcgaggat gccgccaatg cgggcgcctc gctgatcgta    120

tttcccgaag cttatgtacc cggctatccg agttggatct ggcgtctcag gcccggaggc    180

gacatgggac tgtcgtctga gattcacgca agattgcggg aaaatgccgt tgatctcgcg    240

aacggaggcc tggcgcatgt ccagggggct gcagcaaaat tcggcgcgac tgtcgttatc    300

ggcatcaatg aactcgacag cgagttcagc ggaacgacat tgttcaacac cgtggtggtc    360

atcggccccg acggaacgcg cctcaacagg catcgaaaat taatgccgac caacccggag    420

cgcatggtgt ggggcacggg cgatgcctcg ggtctgcgtg tcatcgatac gccggcggga    480

cggctgggaa ccatgatctg ctgggagagc tacatgccgc tggcgcgcta tgctctctat    540

gcgcaaggca tcgagatata cgtcgctccc acgtgggacg caggcgagag ctggattgct    600

acgatgcgcc acatcgccaa ggaggccggc tgctgggtga tcggcacggc aaccgtcatc    660

cagggcagcg atgttccgga cgattttccc gaacgcgaca agctcttcaa gccggaggag    720

tggatcaacg acggcgatgc ggtcgtggtc aagcccatgg gcgcgattgc tgccggaccg    780

cacaatcgac agaaaagcat actctacgcc gacatcgacc gggaggccgc gcggcgagcc    840

cgccggtcgc tcgatgtctg tggccactat tcccgcccag acgttttctc tttctcggtc    900

aaccgaaagc cattccgccc tgccgacttt gtgggttga                           939

<210> 4
<211> 312
<212> PRT
<213> Unknown

<220> 
<223> Obtained from environmental sample

<220> 
<221> DOMAIN
<222> (4)...(192)
<223> Carbon-nitrogen hydrolase

<220> 
<221> SITE
<222> (35)...(50)
<223> Nitrilases / cyanide hydratase signature 1. Prosite id = PS00920

<400> 4
Met Ser Thr Lys Val Ala Ile Val Gln Ala Pro Pro Val Leu Leu His 
1               5                   10                  15      


Arg Asp Arg Thr Ile Ala Lys Val Arg Ser Ser Ile Glu Asp Ala Ala 
            20                  25                  30          


Asn Ala Gly Ala Ser Leu Ile Val Phe Pro Glu Ala Tyr Val Pro Gly 
        35                  40                  45              


Tyr Pro Ser Trp Ile Trp Arg Leu Arg Pro Gly Gly Asp Met Gly Leu 
    50                  55                  60                  


Ser Ser Glu Ile His Ala Arg Leu Arg Glu Asn Ala Val Asp Leu Ala 
65                  70                  75                  80  


Asn Gly Gly Leu Ala His Val Gln Gly Ala Ala Ala Lys Phe Gly Ala 
                85                  90                  95      


Thr Val Val Ile Gly Ile Asn Glu Leu Asp Ser Glu Phe Ser Gly Thr 
            100                 105                 110         


Thr Leu Phe Asn Thr Val Val Val Ile Gly Pro Asp Gly Thr Arg Leu 
        115                 120                 125             


Asn Arg His Arg Lys Leu Met Pro Thr Asn Pro Glu Arg Met Val Trp 
    130                 135                 140                 


Gly Thr Gly Asp Ala Ser Gly Leu Arg Val Ile Asp Thr Pro Ala Gly 
145                 150                 155                 160 


Arg Leu Gly Thr Met Ile Cys Trp Glu Ser Tyr Met Pro Leu Ala Arg 
                165                 170                 175     


Tyr Ala Leu Tyr Ala Gln Gly Ile Glu Ile Tyr Val Ala Pro Thr Trp 
            180                 185                 190         


Asp Ala Gly Glu Ser Trp Ile Ala Thr Met Arg His Ile Ala Lys Glu 
        195                 200                 205             


Ala Gly Cys Trp Val Ile Gly Thr Ala Thr Val Ile Gln Gly Ser Asp 
    210                 215                 220                 


Val Pro Asp Asp Phe Pro Glu Arg Asp Lys Leu Phe Lys Pro Glu Glu 
225                 230                 235                 240 


Trp Ile Asn Asp Gly Asp Ala Val Val Val Lys Pro Met Gly Ala Ile 
                245                 250                 255     


Ala Ala Gly Pro His Asn Arg Gln Lys Ser Ile Leu Tyr Ala Asp Ile 
            260                 265                 270         


Asp Arg Glu Ala Ala Arg Arg Ala Arg Arg Ser Leu Asp Val Cys Gly 
        275                 280                 285             


His Tyr Ser Arg Pro Asp Val Phe Ser Phe Ser Val Asn Arg Lys Pro 
    290                 295                 300                 


Phe Arg Pro Ala Asp Phe Val Gly 
305                 310         


<210> 5
<211> 942
<212> DNA
<213> Unknown

<220> 
<223> Obtained from environmental sample

<400> 5
atgaataaaa agataatagc tgcagctgtt cagatggaag cgaagcttgg tgatatcgat     60

gccaacatcg atgccgcttc caggcttatt gatctcgctg cttcccgcga ggcggaaatt    120

ataattattc cggagttctt cacaacagga atggccttcc atccgtcatt actggatgct    180

gctttaccgt taaagggcag ggcaatggaa tttctaattt caaaagcagg tcagcataat    240

tgcgtggtgg gcggttcatt tctttccatc aaagatgacg gtgaaagata taacacattt    300

gccctggttt ttcctgaggg cagctatgca acccatgata aagacatacc taccatgtgg    360

gagaactgct attataaggg gggacgagat gagggcattt ttgatacacc ccttgggaac    420

tgcggggttg ccctctgctg ggagatggtg aggacaggga ctgtcaaaag actccgttca    480

aggatagatc tattattggc aggctcgtgc tggtggacag ttcctgacgg ctggatgcca    540

tttaagaaga aaatttcaca aatgaacctg gaaatcatga aggatacccc cggcaggatg    600

gcccgtatgc tgggtgttcc tgtaattcat gccgggcatg cgggaggatt caaggggaaa    660

accccttttc tgcctggatt ccccttccgc tcacattgtc tcggagaaac ccagattgtt    720

gacggctccg ggaaaatact ggcaaggctc ccgcgcgaag aaggggaggg tgttatcacc    780

gctgaaatag tcccgggcat gcgggaacca cttgagtctg tacagaagaa gagattctgg    840

ataccggagt ttcatccaat gttccttttt ttctggagat accagaattt tcacggaaag    900

atgtattaca ggctcattac taaaaggcgc actaataaat aa                       942

<210> 6
<211> 313
<212> PRT
<213> Unknown

<220> 
<223> Obtained from environmental sample

<220> 
<221> DOMAIN
<222> (6)...(170)
<223> Carbon-nitrogen hydrolase

<400> 6
Met Asn Lys Lys Ile Ile Ala Ala Ala Val Gln Met Glu Ala Lys Leu 
1               5                   10                  15      


Gly Asp Ile Asp Ala Asn Ile Asp Ala Ala Ser Arg Leu Ile Asp Leu 
            20                  25                  30          


Ala Ala Ser Arg Glu Ala Glu Ile Ile Ile Ile Pro Glu Phe Phe Thr 
        35                  40                  45              


Thr Gly Met Ala Phe His Pro Ser Leu Leu Asp Ala Ala Leu Pro Leu 
    50                  55                  60                  


Lys Gly Arg Ala Met Glu Phe Leu Ile Ser Lys Ala Gly Gln His Asn 
65                  70                  75                  80  


Cys Val Val Gly Gly Ser Phe Leu Ser Ile Lys Asp Asp Gly Glu Arg 
                85                  90                  95      


Tyr Asn Thr Phe Ala Leu Val Phe Pro Glu Gly Ser Tyr Ala Thr His 
            100                 105                 110         


Asp Lys Asp Ile Pro Thr Met Trp Glu Asn Cys Tyr Tyr Lys Gly Gly 
        115                 120                 125             


Arg Asp Glu Gly Ile Phe Asp Thr Pro Leu Gly Asn Cys Gly Val Ala 
    130                 135                 140                 


Leu Cys Trp Glu Met Val Arg Thr Gly Thr Val Lys Arg Leu Arg Ser 
145                 150                 155                 160 


Arg Ile Asp Leu Leu Leu Ala Gly Ser Cys Trp Trp Thr Val Pro Asp 
                165                 170                 175     


Gly Trp Met Pro Phe Lys Lys Lys Ile Ser Gln Met Asn Leu Glu Ile 
            180                 185                 190         


Met Lys Asp Thr Pro Gly Arg Met Ala Arg Met Leu Gly Val Pro Val 
        195                 200                 205             


Ile His Ala Gly His Ala Gly Gly Phe Lys Gly Lys Thr Pro Phe Leu 
    210                 215                 220                 


Pro Gly Phe Pro Phe Arg Ser His Cys Leu Gly Glu Thr Gln Ile Val 
225                 230                 235                 240 


Asp Gly Ser Gly Lys Ile Leu Ala Arg Leu Pro Arg Glu Glu Gly Glu 
                245                 250                 255     


Gly Val Ile Thr Ala Glu Ile Val Pro Gly Met Arg Glu Pro Leu Glu 
            260                 265                 270         


Ser Val Gln Lys Lys Arg Phe Trp Ile Pro Glu Phe His Pro Met Phe 
        275                 280                 285             


Leu Phe Phe Trp Arg Tyr Gln Asn Phe His Gly Lys Met Tyr Tyr Arg 
    290                 295                 300                 


Leu Ile Thr Lys Arg Arg Thr Asn Lys 
305                 310             


<210> 7
<211> 930
<212> DNA
<213> Unknown

<220> 
<223> Obtained from environmental sample

<400> 7
atgagcaagg ttactgtcgg cattattcag gcgcggcccg tgtattacga tctggccggg     60

acgatggaca agaccgccga tctgatcgag gaagcggcga agatggggat caaggtggtc    120

agcctcggcg agacatggct gcccggctac ccggcttggc tggactggtg cgcggactca    180

gcaagatgga atcatccgcc ggtcaaagag gtgtatgcgc ggctgagaca aaatagcgtg    240

gtggtcggcg gcaaagagac tgaccggctg ggtaagctcg ctaagaagca cggcatggtg    300

atcgtggtcg gagtcaacga gcgggtggat cgcgggccgg gcaatgggac tttgtacaac    360

acgcttctga cattcaatgc cgatggggcg ttggtcaacc atcaccgcaa gctggtgccc    420

acctataccg agaagatgat ctgggggcac ggcgacgggc acggcctgca ggccgtcgat    480

acggcggcgg gacgggtgag cgcgctggtg tgctgggaac attggatgcc gctggcgagg    540

caggttctgc acgacagctt tgagcagatc cacgtggcgg cgtggccgag cgtccacgag    600

atgcaccaga tcgccagccg gcagtatgcc ttcgagagcc ggtgttttgt gctggcctgc    660

gggcaaatcc tgcgcgggga cgatctgccc aaagagctgg cgacgccgcc ggaactggcg    720

gatgacccgg acatgctggt gatgaacggc ggcagcgcga tcatcgggcc gaacgggcgg    780

tatctggccg agccggtgta cgaccaggag acgattgtgt gcgccgagat cgatctggac    840

gatatcgatc aggagaaaat gacgctcgat gtgaccgggc attatgcacg gccggatgtg    900

ttcgggttga cggtgaatcg agaacgacag                                     930

<210> 8
<211> 310
<212> PRT
<213> Unknown

<220> 
<223> Obtained from environmental sample

<220> 
<221> DOMAIN
<222> (5)...(196)
<223> Carbon-nitrogen hydrolase

<220> 
<221> SITE
<222> (116)...(119)
<223> N-glycosylation site. Prosite id = PS00001

<400> 8
Met Ser Lys Val Thr Val Gly Ile Ile Gln Ala Arg Pro Val Tyr Tyr 
1               5                   10                  15      


Asp Leu Ala Gly Thr Met Asp Lys Thr Ala Asp Leu Ile Glu Glu Ala 
            20                  25                  30          


Ala Lys Met Gly Ile Lys Val Val Ser Leu Gly Glu Thr Trp Leu Pro 
        35                  40                  45              


Gly Tyr Pro Ala Trp Leu Asp Trp Cys Ala Asp Ser Ala Arg Trp Asn 
    50                  55                  60                  


His Pro Pro Val Lys Glu Val Tyr Ala Arg Leu Arg Gln Asn Ser Val 
65                  70                  75                  80  


Val Val Gly Gly Lys Glu Thr Asp Arg Leu Gly Lys Leu Ala Lys Lys 
                85                  90                  95      


His Gly Met Val Ile Val Val Gly Val Asn Glu Arg Val Asp Arg Gly 
            100                 105                 110         


Pro Gly Asn Gly Thr Leu Tyr Asn Thr Leu Leu Thr Phe Asn Ala Asp 
        115                 120                 125             


Gly Ala Leu Val Asn His His Arg Lys Leu Val Pro Thr Tyr Thr Glu 
    130                 135                 140                 


Lys Met Ile Trp Gly His Gly Asp Gly His Gly Leu Gln Ala Val Asp 
145                 150                 155                 160 


Thr Ala Ala Gly Arg Val Ser Ala Leu Val Cys Trp Glu His Trp Met 
                165                 170                 175     


Pro Leu Ala Arg Gln Val Leu His Asp Ser Phe Glu Gln Ile His Val 
            180                 185                 190         


Ala Ala Trp Pro Ser Val His Glu Met His Gln Ile Ala Ser Arg Gln 
        195                 200                 205             


Tyr Ala Phe Glu Ser Arg Cys Phe Val Leu Ala Cys Gly Gln Ile Leu 
    210                 215                 220                 


Arg Gly Asp Asp Leu Pro Lys Glu Leu Ala Thr Pro Pro Glu Leu Ala 
225                 230                 235                 240 


Asp Asp Pro Asp Met Leu Val Met Asn Gly Gly Ser Ala Ile Ile Gly 
                245                 250                 255     


Pro Asn Gly Arg Tyr Leu Ala Glu Pro Val Tyr Asp Gln Glu Thr Ile 
            260                 265                 270         


Val Cys Ala Glu Ile Asp Leu Asp Asp Ile Asp Gln Glu Lys Met Thr 
        275                 280                 285             


Leu Asp Val Thr Gly His Tyr Ala Arg Pro Asp Val Phe Gly Leu Thr 
    290                 295                 300                 


Val Asn Arg Glu Arg Gln 
305                 310 


<210> 9
<211> 705
<212> DNA
<213> Unknown

<220> 
<223> Obtained from environmental sample

<400> 9
gtccgcccac atgaacgcga tcacggcacg ctttacaaca cccaattggt atttgatgcc     60

agcggcgaac tggtgttgaa gcggcgcaaa atcacgccaa cctaccatga acgtatgatc    120

tggggacagg gtgatggtgc cgggctgaag gtggtcgaca ccgccgtggg gcgcatcggc    180

gcgctggcgt gctgggagca ttacaacccg ctggcacgct acagcctgat gacgcagcac    240

gaggagatcc actgtagtca attccctggt tctctggttg gaccgatttt cgccgagcag    300

atggacgtca cgatccgcca tcacgcgctg gagtctggct gctttgtcat caatgccacc    360

ggctggctga ctgaggagca aatcaacgaa ctcacctctg acccggcatt gcagaaaggg    420

ctaaggggag gctgcaatac cgccatcatt tcgccggaag gacgccatct ggtgcccccg    480

ttgacggaag gcgaaggcat tctgattgcc gatctggata tggcgttgat caccaaacgc    540

aaacgcatga tggattccgt cgggcattac gcacgtccgg aattattgag cctgcgtctg    600

gacgcgaccc ccgcccgcta cgtggtcgcg cgtgatgaag aatccgcaac cggaggagag    660

cgcgatgcag aacgttccgt ctactcgcca gcagctgatc actga                    705

<210> 10
<211> 234
<212> PRT
<213> Unknown

<220> 
<223> Obtained from environmental sample

<220> 
<221> SITE
<222> (119)...(122)
<223> N-glycosylation site. Prosite id = PS00001

<400> 10
Val Arg Pro His Glu Arg Asp His Gly Thr Leu Tyr Asn Thr Gln Leu 
1               5                   10                  15      


Val Phe Asp Ala Ser Gly Glu Leu Val Leu Lys Arg Arg Lys Ile Thr 
            20                  25                  30          


Pro Thr Tyr His Glu Arg Met Ile Trp Gly Gln Gly Asp Gly Ala Gly 
        35                  40                  45              


Leu Lys Val Val Asp Thr Ala Val Gly Arg Ile Gly Ala Leu Ala Cys 
    50                  55                  60                  


Trp Glu His Tyr Asn Pro Leu Ala Arg Tyr Ser Leu Met Thr Gln His 
65                  70                  75                  80  


Glu Glu Ile His Cys Ser Gln Phe Pro Gly Ser Leu Val Gly Pro Ile 
                85                  90                  95      


Phe Ala Glu Gln Met Asp Val Thr Ile Arg His His Ala Leu Glu Ser 
            100                 105                 110         


Gly Cys Phe Val Ile Asn Ala Thr Gly Trp Leu Thr Glu Glu Gln Ile 
        115                 120                 125             


Asn Glu Leu Thr Ser Asp Pro Ala Leu Gln Lys Gly Leu Arg Gly Gly 
    130                 135                 140                 


Cys Asn Thr Ala Ile Ile Ser Pro Glu Gly Arg His Leu Val Pro Pro 
145                 150                 155                 160 


Leu Thr Glu Gly Glu Gly Ile Leu Ile Ala Asp Leu Asp Met Ala Leu 
                165                 170                 175     


Ile Thr Lys Arg Lys Arg Met Met Asp Ser Val Gly His Tyr Ala Arg 
            180                 185                 190         


Pro Glu Leu Leu Ser Leu Arg Leu Asp Ala Thr Pro Ala Arg Tyr Val 
        195                 200                 205             


Val Ala Arg Asp Glu Glu Ser Ala Thr Gly Gly Glu Arg Asp Ala Glu 
    210                 215                 220                 


Arg Ser Val Tyr Ser Pro Ala Ala Asp His 
225                 230                 


<210> 11
<211> 972
<212> DNA
<213> Unknown

<220> 
<223> Obtained from environmental sample

<400> 11
atgaaaactc aaagtggaga ccatctgaaa gtcggccttg cccagatctc cccggtatgg     60

ctcaataaaa agcttaccct ggaaaaggtg tcgtcatttg tggacatggc tggatcggag    120

ggttgcgatc tcgtggtctt tggcgaaggg ctggtccccg gatatccatt ctggttgggt    180

cttacggatg gggccaaatt caatgatccg atccagaagg acatccatgc ccattacatc    240

cggaatgccg tggagatcga aaaaggtgat ctggacagga tctgtgaact ggcttcaaaa    300

tggcaactgg ggatctatct ggggaccatt gagagagcgc aggaccgggg aaatcatagc    360

ctgtattgca gcctggtcta catagatact acgggagcca ttcgatcggt ccatcgaaaa    420

ttgaggccca cttatgaaga aagattagcc tgggcaccgg gggacggtca tggtctgcag    480

gtccatgctc tcaaggcctt taccgttggg ggactgaatt gctgggaaaa ctggatgcct    540

atgccccgca tggcattata cggacagggt gaaaaccttc atgtagccgt ttggccgggt    600

tccgtccata atacggagga tataacccgg ttcatcgcga aagaggcccg ttcgtacgtg    660

atctccgttt ccggactaat gacacgtcac gactttcctt tcgacacacc tcatctggac    720

cggatactgg ctcaggcacc ggaacatctc acgaacggag gctcttgtct ggcggggccg    780

gatggccaat ggatcattga accggtactt catgaagaat gtctgcgaac tgccgagatc    840

gatatcaacc gggtccttga ggagcggcag aatatggacc tttcgggcca ttattccaga    900

ccggatgtac ttaaacttat gatcaaccgg gagcgacaat ccgtagtcgg atttcaggac    960

ccagagaagt ag                                                        972

<210> 12
<211> 323
<212> PRT
<213> Unknown

<220> 
<223> Obtained from environmental sample

<220> 
<221> DOMAIN
<222> (10)...(199)
<223> Carbon-nitrogen hydrolase

<220> 
<221> SITE
<222> (41)...(56)
<223> Nitrilases / cyanide hydratase signature 1. Prosite id = PS00920

<220> 
<221> SITE
<222> (119)...(122)
<223> N-glycosylation site. Prosite id = PS00001

<220> 
<221> SITE
<222> (172)...(185)
<223> Nitrilases / cyanide hydratase active site signature. Prosite id = PS00921

<400> 12
Met Lys Thr Gln Ser Gly Asp His Leu Lys Val Gly Leu Ala Gln Ile 
1               5                   10                  15      


Ser Pro Val Trp Leu Asn Lys Lys Leu Thr Leu Glu Lys Val Ser Ser 
            20                  25                  30          


Phe Val Asp Met Ala Gly Ser Glu Gly Cys Asp Leu Val Val Phe Gly 
        35                  40                  45              


Glu Gly Leu Val Pro Gly Tyr Pro Phe Trp Leu Gly Leu Thr Asp Gly 
    50                  55                  60                  


Ala Lys Phe Asn Asp Pro Ile Gln Lys Asp Ile His Ala His Tyr Ile 
65                  70                  75                  80  


Arg Asn Ala Val Glu Ile Glu Lys Gly Asp Leu Asp Arg Ile Cys Glu 
                85                  90                  95      


Leu Ala Ser Lys Trp Gln Leu Gly Ile Tyr Leu Gly Thr Ile Glu Arg 
            100                 105                 110         


Ala Gln Asp Arg Gly Asn His Ser Leu Tyr Cys Ser Leu Val Tyr Ile 
        115                 120                 125             


Asp Thr Thr Gly Ala Ile Arg Ser Val His Arg Lys Leu Arg Pro Thr 
    130                 135                 140                 


Tyr Glu Glu Arg Leu Ala Trp Ala Pro Gly Asp Gly His Gly Leu Gln 
145                 150                 155                 160 


Val His Ala Leu Lys Ala Phe Thr Val Gly Gly Leu Asn Cys Trp Glu 
                165                 170                 175     


Asn Trp Met Pro Met Pro Arg Met Ala Leu Tyr Gly Gln Gly Glu Asn 
            180                 185                 190         


Leu His Val Ala Val Trp Pro Gly Ser Val His Asn Thr Glu Asp Ile 
        195                 200                 205             


Thr Arg Phe Ile Ala Lys Glu Ala Arg Ser Tyr Val Ile Ser Val Ser 
    210                 215                 220                 


Gly Leu Met Thr Arg His Asp Phe Pro Phe Asp Thr Pro His Leu Asp 
225                 230                 235                 240 


Arg Ile Leu Ala Gln Ala Pro Glu His Leu Thr Asn Gly Gly Ser Cys 
                245                 250                 255     


Leu Ala Gly Pro Asp Gly Gln Trp Ile Ile Glu Pro Val Leu His Glu 
            260                 265                 270         


Glu Cys Leu Arg Thr Ala Glu Ile Asp Ile Asn Arg Val Leu Glu Glu 
        275                 280                 285             


Arg Gln Asn Met Asp Leu Ser Gly His Tyr Ser Arg Pro Asp Val Leu 
    290                 295                 300                 


Lys Leu Met Ile Asn Arg Glu Arg Gln Ser Val Val Gly Phe Gln Asp 
305                 310                 315                 320 


Pro Glu Lys 
            


<210> 13
<211> 1020
<212> DNA
<213> Unknown

<220> 
<223> Obtained from environmental sample

<400> 13
gtgacgaaca ccatcaaggt tgccgcggcc cactacgcgc cggtactgct cgacgtcggt     60

gcaaccatcg acaaggcctg cgccgtgatc gaggaggcgg gtcgggccgg cgttggcctg    120

ctggtcttcc cggaggtgtt cgttccgggg tttccctact ggatcaactg ttacccgcct    180

ctggtacagg ccgggctgaa ccgccggtac caggactgct ccatcgaaat tcccggcccg    240

gagattgccc aggtgcgggc cgcggccggc cgcgccggtg tcgtgaccgt gctcgggatc    300

agcgagcgtg ccggcggcgg gcggacttgt tacaacagct gcgtggtgat cgacgccgac    360

ggcagcctcg ccggggtcca ccgcaagctc cagccgacct atgccgagcg catgatctgg    420

ggtcaggggg acggatctac ccttaacgtg tatgacaccg gggtcgggag agtcgggact    480

ctcgcctgct gggagcacac gctgaacctt gcgcgccacg cgctggtgct tcagtcggag    540

cagatccacg cggcgctctg gccgggcctc tcgacgatcg ccgggttccg cgaactcgcc    600

gatgatcagg tcgaggccat gatgaagaat cacgccatca cgggtcagtg tttcgtcgtg    660

tccgccgcca accctgtcac ccagcagatg ctcgacgtga tggagcaggc gctcggtccg    720

caggagttca ccggggcagg cggtggctgg tcggcgatcg tgcatccgtt cacggctttc    780

tgtacgccac cgcacacggg ccccgaggag cgcctcgtca cggccgatat cgatcttgat    840

gacatcaagg acgtcaagat gtgggtcgac agtgctggac actactcacg ccccgaggtg    900

ctcggactgt ggttcgaccg ccagcccaag catcccctgg tgggtcgtcg cttgcccgag    960

ccgccggagg ctccggaaga atccgacgac gaacagacgc agagcgggac tcagcagtag   1020

<210> 14
<211> 339
<212> PRT
<213> Unknown

<220> 
<223> Obtained from environmental sample

<220> 
<221> DOMAIN
<222> (6)...(188)
<223> Carbon-nitrogen hydrolase

<400> 14
Met Thr Asn Thr Ile Lys Val Ala Ala Ala His Tyr Ala Pro Val Leu 
1               5                   10                  15      


Leu Asp Val Gly Ala Thr Ile Asp Lys Ala Cys Ala Val Ile Glu Glu 
            20                  25                  30          


Ala Gly Arg Ala Gly Val Gly Leu Leu Val Phe Pro Glu Val Phe Val 
        35                  40                  45              


Pro Gly Phe Pro Tyr Trp Ile Asn Cys Tyr Pro Pro Leu Val Gln Ala 
    50                  55                  60                  


Gly Leu Asn Arg Arg Tyr Gln Asp Cys Ser Ile Glu Ile Pro Gly Pro 
65                  70                  75                  80  


Glu Ile Ala Gln Val Arg Ala Ala Ala Gly Arg Ala Gly Val Val Thr 
                85                  90                  95      


Val Leu Gly Ile Ser Glu Arg Ala Gly Gly Gly Arg Thr Cys Tyr Asn 
            100                 105                 110         


Ser Cys Val Val Ile Asp Ala Asp Gly Ser Leu Ala Gly Val His Arg 
        115                 120                 125             


Lys Leu Gln Pro Thr Tyr Ala Glu Arg Met Ile Trp Gly Gln Gly Asp 
    130                 135                 140                 


Gly Ser Thr Leu Asn Val Tyr Asp Thr Gly Val Gly Arg Val Gly Thr 
145                 150                 155                 160 


Leu Ala Cys Trp Glu His Thr Leu Asn Leu Ala Arg His Ala Leu Val 
                165                 170                 175     


Leu Gln Ser Glu Gln Ile His Ala Ala Leu Trp Pro Gly Leu Ser Thr 
            180                 185                 190         


Ile Ala Gly Phe Arg Glu Leu Ala Asp Asp Gln Val Glu Ala Met Met 
        195                 200                 205             


Lys Asn His Ala Ile Thr Gly Gln Cys Phe Val Val Ser Ala Ala Asn 
    210                 215                 220                 


Pro Val Thr Gln Gln Met Leu Asp Val Met Glu Gln Ala Leu Gly Pro 
225                 230                 235                 240 


Gln Glu Phe Thr Gly Ala Gly Gly Gly Trp Ser Ala Ile Val His Pro 
                245                 250                 255     


Phe Thr Ala Phe Cys Thr Pro Pro His Thr Gly Pro Glu Glu Arg Leu 
            260                 265                 270         


Val Thr Ala Asp Ile Asp Leu Asp Asp Ile Lys Asp Val Lys Met Trp 
        275                 280                 285             


Val Asp Ser Ala Gly His Tyr Ser Arg Pro Glu Val Leu Gly Leu Trp 
    290                 295                 300                 


Phe Asp Arg Gln Pro Lys His Pro Leu Val Gly Arg Arg Leu Pro Glu 
305                 310                 315                 320 


Pro Pro Glu Ala Pro Glu Glu Ser Asp Asp Glu Gln Thr Gln Ser Gly 
                325                 330                 335     


Thr Gln Gln 
            


<210> 15
<211> 957
<212> DNA
<213> Unknown

<220> 
<223> Obtained from environmental sample

<400> 15
atgtctcaaa ataatatcct taccgttggc cttgcacagc ttgcgccagt ttggctcaat     60

aaaaatgcaa cattgcaaaa agcggccgag gcaatccggg aagctgctca aaatgaatgt    120

gaactggtgg tattcggtga gggatttgtt cccggctatc cacactggct ggcaaatact    180

tccgcatccg agtggaattc tccggttcaa aaagagctcc atgcccatta tgcacgaaat    240

gccgtacaga ttgaatccgg tgatcttgat gatatctgtt cactcgctaa atctttgaac    300

atagcggtat acctcggcat tattgaacgt gcggccgacc gtggcggaca cagtctctac    360

tgctccctgg tgtatattga ccaaagcgga accattcaat ccgtgcaccg aaagctgcag    420

ccaacgtatg atgaacgcct gacttggtcg ccgggagatg gccatggtct tcgagtgcat    480

aagctcaaaa attttactgt gggcggactg aattgctggg aaaactggat gcctcttccg    540

cgtgcagcac tctatggact gggtgaaaat ctgcatatag ccgtttggcc ggggagtgta    600

cacaacacaa aagatatcac acgttttatt gcacgcgaaa gccggtcata cgtaatttca    660

gtatcgtcac tgatgcgaaa acaggatttc ccggataata caccccacct tagtgaaatt    720

cttaaaaaag cccctgatgt tctgacaaat gggggctcct gcattgccgg ccctgacggt    780

aactggattg ttgaacctgt ggcggataaa gaaggattga ttaccggaac tcttgatttc    840

aatcgcatat acgaagaaag gcaaaatttt gatccatcgg gacattattc ccgacccgat    900

gtaacccgac ttaccattaa ccgggaacgc cagtctacaa ttgatctcga ggattaa       957

<210> 16
<211> 318
<212> PRT
<213> Unknown

<220> 
<223> Obtained from environmental sample

<220> 
<221> DOMAIN
<222> (8)...(197)
<223> Carbon-nitrogen hydrolase

<220> 
<221> SITE
<222> (22)...(25)
<223> N-glycosylation site. Prosite id = PS00001

<220> 
<221> SITE
<222> (59)...(62)
<223> N-glycosylation site. Prosite id = PS00001

<220> 
<221> SITE
<222> (166)...(169)
<223> N-glycosylation site. Prosite id = PS00001

<220> 
<221> SITE
<222> (170)...(183)
<223> Nitrilases / cyanide hydratase active site signature. Prosite id = PS00921

<400> 16
Met Ser Gln Asn Asn Ile Leu Thr Val Gly Leu Ala Gln Leu Ala Pro 
1               5                   10                  15      


Val Trp Leu Asn Lys Asn Ala Thr Leu Gln Lys Ala Ala Glu Ala Ile 
            20                  25                  30          


Arg Glu Ala Ala Gln Asn Glu Cys Glu Leu Val Val Phe Gly Glu Gly 
        35                  40                  45              


Phe Val Pro Gly Tyr Pro His Trp Leu Ala Asn Thr Ser Ala Ser Glu 
    50                  55                  60                  


Trp Asn Ser Pro Val Gln Lys Glu Leu His Ala His Tyr Ala Arg Asn 
65                  70                  75                  80  


Ala Val Gln Ile Glu Ser Gly Asp Leu Asp Asp Ile Cys Ser Leu Ala 
                85                  90                  95      


Lys Ser Leu Asn Ile Ala Val Tyr Leu Gly Ile Ile Glu Arg Ala Ala 
            100                 105                 110         


Asp Arg Gly Gly His Ser Leu Tyr Cys Ser Leu Val Tyr Ile Asp Gln 
        115                 120                 125             


Ser Gly Thr Ile Gln Ser Val His Arg Lys Leu Gln Pro Thr Tyr Asp 
    130                 135                 140                 


Glu Arg Leu Thr Trp Ser Pro Gly Asp Gly His Gly Leu Arg Val His 
145                 150                 155                 160 


Lys Leu Lys Asn Phe Thr Val Gly Gly Leu Asn Cys Trp Glu Asn Trp 
                165                 170                 175     


Met Pro Leu Pro Arg Ala Ala Leu Tyr Gly Leu Gly Glu Asn Leu His 
            180                 185                 190         


Ile Ala Val Trp Pro Gly Ser Val His Asn Thr Lys Asp Ile Thr Arg 
        195                 200                 205             


Phe Ile Ala Arg Glu Ser Arg Ser Tyr Val Ile Ser Val Ser Ser Leu 
    210                 215                 220                 


Met Arg Lys Gln Asp Phe Pro Asp Asn Thr Pro His Leu Ser Glu Ile 
225                 230                 235                 240 


Leu Lys Lys Ala Pro Asp Val Leu Thr Asn Gly Gly Ser Cys Ile Ala 
                245                 250                 255     


Gly Pro Asp Gly Asn Trp Ile Val Glu Pro Val Ala Asp Lys Glu Gly 
            260                 265                 270         


Leu Ile Thr Gly Thr Leu Asp Phe Asn Arg Ile Tyr Glu Glu Arg Gln 
        275                 280                 285             


Asn Phe Asp Pro Ser Gly His Tyr Ser Arg Pro Asp Val Thr Arg Leu 
    290                 295                 300                 


Thr Ile Asn Arg Glu Arg Gln Ser Thr Ile Asp Leu Glu Asp 
305                 310                 315             


<210> 17
<211> 1020
<212> DNA
<213> Unknown

<220> 
<223> Obtained from environmental sample

<400> 17
gtgacgaaca ccatcaaggt tgctgctgcc cactacgcgc cggtgctgct ggatgtcggt     60

gccaccatcg acaaggcctg cgcggtgatc gaggaggccg gccgggccgg tgtcggtctg    120

ctggtcttcc cggaagtgtt cgttccgggg tttccctact ggatcaactg ctacccgccg    180

ctggtgcagg ccgggctgaa ccgccgctac caggactgct cgatcgaggt tcccggcccg    240

gagatcgccc gggtgcaggc cgctgcggcc cgggcgggcg tcgtgaccgt gctcgggatc    300

agcgagcgcg cccgcggcgg gcgcacctgt tacaacagct gcgtggtgat cgacgccgac    360

ggcaccgtgg ccggcgtcca tcgcaagctg cagccgacct atgccgagcg catgatctgg    420

ggtcaggggg acggctccac tctcagcgtc tatgaaaccg gggtcgggag ggtggggacg    480

ctcgcctgct gggaacacac gctcaatctc gcgcgccacg cgctggtgct gcagtccgag    540

cagatccacg cagcgctctg gccgggcctg tcgaccatcg ccgggttccg cgaactggcc    600

gatgtccaga tcgaggccat gatgaggaac cacgccatca ccggtcagtg tttcgtcgtg    660

tccgcggcca acccggtcac ccagcagatg ctcgacgtga tggagcaggc gctcggcccg    720

caggagttca ccggggcagg cggtggctgg tcggcgatcg tgcatccgtt cacggccttc    780

tgcgcgccac cgcacacagg tcccgaggag cgcctggtca cggcggacat caatctcgac    840

gacatcaagg acgtcaagat gtgggtcgac agcgccgggc actactcacg ccctgaggtg    900

ctcgggctgt ggttcgaccg tcagcccaag cgcccgctgg tgggccgtcg cctgccggag    960

acggcggagg gcccggaaga gaccgacgac gagcagccgc agaccggaac tcagcagtag   1020

<210> 18
<211> 339
<212> PRT
<213> Unknown

<220> 
<223> Obtained from environmental sample

<220> 
<221> DOMAIN
<222> (6)...(188)
<223> Carbon-nitrogen hydrolase

<400> 18
Met Thr Asn Thr Ile Lys Val Ala Ala Ala His Tyr Ala Pro Val Leu 
1               5                   10                  15      


Leu Asp Val Gly Ala Thr Ile Asp Lys Ala Cys Ala Val Ile Glu Glu 
            20                  25                  30          


Ala Gly Arg Ala Gly Val Gly Leu Leu Val Phe Pro Glu Val Phe Val 
        35                  40                  45              


Pro Gly Phe Pro Tyr Trp Ile Asn Cys Tyr Pro Pro Leu Val Gln Ala 
    50                  55                  60                  


Gly Leu Asn Arg Arg Tyr Gln Asp Cys Ser Ile Glu Val Pro Gly Pro 
65                  70                  75                  80  


Glu Ile Ala Arg Val Gln Ala Ala Ala Ala Arg Ala Gly Val Val Thr 
                85                  90                  95      


Val Leu Gly Ile Ser Glu Arg Ala Arg Gly Gly Arg Thr Cys Tyr Asn 
            100                 105                 110         


Ser Cys Val Val Ile Asp Ala Asp Gly Thr Val Ala Gly Val His Arg 
        115                 120                 125             


Lys Leu Gln Pro Thr Tyr Ala Glu Arg Met Ile Trp Gly Gln Gly Asp 
    130                 135                 140                 


Gly Ser Thr Leu Ser Val Tyr Glu Thr Gly Val Gly Arg Val Gly Thr 
145                 150                 155                 160 


Leu Ala Cys Trp Glu His Thr Leu Asn Leu Ala Arg His Ala Leu Val 
                165                 170                 175     


Leu Gln Ser Glu Gln Ile His Ala Ala Leu Trp Pro Gly Leu Ser Thr 
            180                 185                 190         


Ile Ala Gly Phe Arg Glu Leu Ala Asp Val Gln Ile Glu Ala Met Met 
        195                 200                 205             


Arg Asn His Ala Ile Thr Gly Gln Cys Phe Val Val Ser Ala Ala Asn 
    210                 215                 220                 


Pro Val Thr Gln Gln Met Leu Asp Val Met Glu Gln Ala Leu Gly Pro 
225                 230                 235                 240 


Gln Glu Phe Thr Gly Ala Gly Gly Gly Trp Ser Ala Ile Val His Pro 
                245                 250                 255     


Phe Thr Ala Phe Cys Ala Pro Pro His Thr Gly Pro Glu Glu Arg Leu 
            260                 265                 270         


Val Thr Ala Asp Ile Asn Leu Asp Asp Ile Lys Asp Val Lys Met Trp 
        275                 280                 285             


Val Asp Ser Ala Gly His Tyr Ser Arg Pro Glu Val Leu Gly Leu Trp 
    290                 295                 300                 


Phe Asp Arg Gln Pro Lys Arg Pro Leu Val Gly Arg Arg Leu Pro Glu 
305                 310                 315                 320 


Thr Ala Glu Gly Pro Glu Glu Thr Asp Asp Glu Gln Pro Gln Thr Gly 
                325                 330                 335     


Thr Gln Gln 
            


<210> 19
<211> 1005
<212> DNA
<213> Unknown

<220> 
<223> Obtained from environmental sample

<400> 19
atgagtcgaa tcgtaaaagc tgcagccgta cagtgtagtc cagtgcttta tagccaagca     60

ggcaccgtga aaaaggtctg tgagctaatt gctgagcttg gacagcaagg tgtgcaattt    120

gcagtttttc ctgagactgt ggtgccgtat tacccttatt tttcctttgt ccagcctccg    180

tttgcgatgg gtaaagaaca tctcaaacta ctcaatgaat ctgtggtggt gccttcagaa    240

gccacagatc tcattggcgc tgcatgtcgt gctgcgcaga tggtggtttc cattggaatt    300

aatgaacgtg caggcggcac catttataac gcccagctgt tatttgatac ggatggcagc    360

atcattcagc atcgtcgcaa aattacgccg acctatcatg aacgtatggt ttgggggcag    420

ggtgatggca gtggtttatg tgcgattgat tcggctgttg gacgaattgg ttcattggct    480

tgttgggagc attacaaccc attggcacgt tttgcgctta tggctgatgg cgaacagatc    540

catgcggcta tgttccctgg atcattggtc ggacaagttt ttgccgatca gatctctgca    600

acgattcaac atcatgcttt ggaatcaggc tgctttgttg tgaatgccac agcatggctg    660

acaccggaac aacagcaaca gattatgcag gatactggtt gtgaaatagg tccaatctca    720

ggtggatgct ttactgccat tgtttcacca gaaggcaaat ttttagctgc acctttaaca    780

gagggtgagg gctatgtgat tgctgatctg gactttagcc tgattgataa acgtaaacgt    840

atgatggact ctgtggggca ttacagtcgc cctgaattgc tgagcctgct gatagatcgt    900

cgcaaaaccc aggttttaca tgaatatcca gaatctcaat caacaacaca agaaaaaatc    960

agcaacttta ccgcgccaac agaagagcag ctgagtaaag cgtag                   1005

<210> 20
<211> 334
<212> PRT
<213> Unknown

<220> 
<223> Obtained from environmental sample

<220> 
<221> SIGNAL
<222> (1)...(20)

<220> 
<221> DOMAIN
<222> (6)...(186)
<223> Carbon-nitrogen hydrolase

<220> 
<221> SITE
<222> (73)...(76)
<223> N-glycosylation site. Prosite id = PS00001

<220> 
<221> SITE
<222> (218)...(221)
<223> N-glycosylation site. Prosite id = PS00001

<220> 
<221> SITE
<222> (326)...(329)
<223> N-glycosylation site. Prosite id = PS00001

<400> 20
Met Ser Arg Ile Val Lys Ala Ala Ala Val Gln Cys Ser Pro Val Leu 
1               5                   10                  15      


Tyr Ser Gln Ala Gly Thr Val Lys Lys Val Cys Glu Leu Ile Ala Glu 
            20                  25                  30          


Leu Gly Gln Gln Gly Val Gln Phe Ala Val Phe Pro Glu Thr Val Val 
        35                  40                  45              


Pro Tyr Tyr Pro Tyr Phe Ser Phe Val Gln Pro Pro Phe Ala Met Gly 
    50                  55                  60                  


Lys Glu His Leu Lys Leu Leu Asn Glu Ser Val Val Val Pro Ser Glu 
65                  70                  75                  80  


Ala Thr Asp Leu Ile Gly Ala Ala Cys Arg Ala Ala Gln Met Val Val 
                85                  90                  95      


Ser Ile Gly Ile Asn Glu Arg Ala Gly Gly Thr Ile Tyr Asn Ala Gln 
            100                 105                 110         


Leu Leu Phe Asp Thr Asp Gly Ser Ile Ile Gln His Arg Arg Lys Ile 
        115                 120                 125             


Thr Pro Thr Tyr His Glu Arg Met Val Trp Gly Gln Gly Asp Gly Ser 
    130                 135                 140                 


Gly Leu Cys Ala Ile Asp Ser Ala Val Gly Arg Ile Gly Ser Leu Ala 
145                 150                 155                 160 


Cys Trp Glu His Tyr Asn Pro Leu Ala Arg Phe Ala Leu Met Ala Asp 
                165                 170                 175     


Gly Glu Gln Ile His Ala Ala Met Phe Pro Gly Ser Leu Val Gly Gln 
            180                 185                 190         


Val Phe Ala Asp Gln Ile Ser Ala Thr Ile Gln His His Ala Leu Glu 
        195                 200                 205             


Ser Gly Cys Phe Val Val Asn Ala Thr Ala Trp Leu Thr Pro Glu Gln 
    210                 215                 220                 


Gln Gln Gln Ile Met Gln Asp Thr Gly Cys Glu Ile Gly Pro Ile Ser 
225                 230                 235                 240 


Gly Gly Cys Phe Thr Ala Ile Val Ser Pro Glu Gly Lys Phe Leu Ala 
                245                 250                 255     


Ala Pro Leu Thr Glu Gly Glu Gly Tyr Val Ile Ala Asp Leu Asp Phe 
            260                 265                 270         


Ser Leu Ile Asp Lys Arg Lys Arg Met Met Asp Ser Val Gly His Tyr 
        275                 280                 285             


Ser Arg Pro Glu Leu Leu Ser Leu Leu Ile Asp Arg Arg Lys Thr Gln 
    290                 295                 300                 


Val Leu His Glu Tyr Pro Glu Ser Gln Ser Thr Thr Gln Glu Lys Ile 
305                 310                 315                 320 


Ser Asn Phe Thr Ala Pro Thr Glu Glu Gln Leu Ser Lys Ala 
                325                 330                 


<210> 21
<211> 1020
<212> DNA
<213> Unknown

<220> 
<223> Obtained from environmental sample

<400> 21
gtgacgaaca ccatcaaggt tgcggctgcc cactacgcgc cggtgctgct ggatgtcggt     60

gccaccatcg acaaggcctg cgcggtgatc gaggaggccg gccgggccgg tgtcggtctg    120

ctggtcttcc cggaagtgtt cgttccgggg tttccctact ggatcaactg ctacccgccg    180

ctggtgcagg ccgggctgaa ccgccgctac caggactgct cgatcgaggt tcccggcccg    240

gagatcgccc gggtgcaggc cgctgcggcc cgggcgggcg tcgtgaccgt gctcgggatc    300

agcgagcgcg cccgcggcgg gcgcacctgt tacaacagct gcgtggtgat cgatgccgac    360

ggcaccgtgg ccggcgtcca tcgcaagctg cagccgacct atgccgagcg catgatctgg    420

ggtcaggggg acggctccac cctcagcgtc tatgaaaccg gggtcgggag ggtggggacg    480

ctcgcctgct gggaacacac gctcaatctc gcgcgccacg cgctggtgct gcagtccgag    540

cagatccacg cggcgctctg gccgggcctg tcgaccatcg ccgggttccg cgaactggcc    600

gatgtccaga tcgaggccat gatgaggaac cacgccatca ccggtcagtg tttcgtcgtg    660

tccgcggcca acccggtcac ccagcagatg ctcgacgtga tggagcaggc gctcggcccg    720

caggagttca ccggggcagg cggtggctgg tcggccatcg tgcatccgtt cacggccttc    780

tgcacgccac cgcacacggg tcccgaggag cgcctggtca cggcggacat caatctcgac    840

gacatcaagg acgtcaagat gtgggtcgac agcgcagggc actactcacg ccccgaggtg    900

ctcgggctgt ggttcgaccg tcagcccaag cgcccgctgg tgggccgtcg cctgccggag    960

acggcggagg gtccggaaga aaccgacgac gagcagccgc agagcgggac tcagcagtag   1020

<210> 22
<211> 339
<212> PRT
<213> Unknown

<220> 
<223> Obtained from environmental sample

<220> 
<221> DOMAIN
<222> (6)...(188)
<223> Carbon-nitrogen hydrolase

<400> 22
Met Thr Asn Thr Ile Lys Val Ala Ala Ala His Tyr Ala Pro Val Leu 
1               5                   10                  15      


Leu Asp Val Gly Ala Thr Ile Asp Lys Ala Cys Ala Val Ile Glu Glu 
            20                  25                  30          


Ala Gly Arg Ala Gly Val Gly Leu Leu Val Phe Pro Glu Val Phe Val 
        35                  40                  45              


Pro Gly Phe Pro Tyr Trp Ile Asn Cys Tyr Pro Pro Leu Val Gln Ala 
    50                  55                  60                  


Gly Leu Asn Arg Arg Tyr Gln Asp Cys Ser Ile Glu Val Pro Gly Pro 
65                  70                  75                  80  


Glu Ile Ala Arg Val Gln Ala Ala Ala Ala Arg Ala Gly Val Val Thr 
                85                  90                  95      


Val Leu Gly Ile Ser Glu Arg Ala Arg Gly Gly Arg Thr Cys Tyr Asn 
            100                 105                 110         


Ser Cys Val Val Ile Asp Ala Asp Gly Thr Val Ala Gly Val His Arg 
        115                 120                 125             


Lys Leu Gln Pro Thr Tyr Ala Glu Arg Met Ile Trp Gly Gln Gly Asp 
    130                 135                 140                 


Gly Ser Thr Leu Ser Val Tyr Glu Thr Gly Val Gly Arg Val Gly Thr 
145                 150                 155                 160 


Leu Ala Cys Trp Glu His Thr Leu Asn Leu Ala Arg His Ala Leu Val 
                165                 170                 175     


Leu Gln Ser Glu Gln Ile His Ala Ala Leu Trp Pro Gly Leu Ser Thr 
            180                 185                 190         


Ile Ala Gly Phe Arg Glu Leu Ala Asp Val Gln Ile Glu Ala Met Met 
        195                 200                 205             


Arg Asn His Ala Ile Thr Gly Gln Cys Phe Val Val Ser Ala Ala Asn 
    210                 215                 220                 


Pro Val Thr Gln Gln Met Leu Asp Val Met Glu Gln Ala Leu Gly Pro 
225                 230                 235                 240 


Gln Glu Phe Thr Gly Ala Gly Gly Gly Trp Ser Ala Ile Val His Pro 
                245                 250                 255     


Phe Thr Ala Phe Cys Thr Pro Pro His Thr Gly Pro Glu Glu Arg Leu 
            260                 265                 270         


Val Thr Ala Asp Ile Asn Leu Asp Asp Ile Lys Asp Val Lys Met Trp 
        275                 280                 285             


Val Asp Ser Ala Gly His Tyr Ser Arg Pro Glu Val Leu Gly Leu Trp 
    290                 295                 300                 


Phe Asp Arg Gln Pro Lys Arg Pro Leu Val Gly Arg Arg Leu Pro Glu 
305                 310                 315                 320 


Thr Ala Glu Gly Pro Glu Glu Thr Asp Asp Glu Gln Pro Gln Ser Gly 
                325                 330                 335     


Thr Gln Gln 
            


<210> 23
<211> 1020
<212> DNA
<213> Unknown

<220> 
<223> Obtained from environmental sample

<400> 23
atgacgcaat attcaaaatt caaagtcgcc gcgattcagg cggcgccagt gtatttcaac     60

cctgaagcca gcaccgcgaa agcgtgtcac ctgatcgagt cggccgggca aaagggtgtg    120

gagctggccg cttttggcga aacatggctg cccggttacc cgttctttgt ctggggggcg    180

ggacgcggcc gggcggcctg gaaggcgcag gccgcctatc tgtccgccgc cgtggagatt    240

ccctctcccg tcacggagca actgtgcgcg gcggctcgac gcgccagcat cgacgtggtg    300

atcggggtgg cggaaaggga ttcgctcagc aggggaacgg tctactgcac gctgctgttc    360

atcagtcgag aaggcagtat tctgggccgc catcgcaaac tgcggccgac ccacaaggaa    420

agaaccgcct ggggagatgg cgacgccgtc ggtttgtcgg tatatgagcg accttacggc    480

agaatcagcg ggctgaactg ctgggaacac aatatggtgc ttcccggcta cgccttgatg    540

actcaaggca ctcagatcca tgtcgccgcc tggcccggca cggaagcccc tgccccgccc    600

gcccccatcc ccatgtggga gcgacaactc cttctgtcgc gcgccttcgc ctcccaggcg    660

gccgcctacg tcatccaggc cggcggcctg ctgtccgagg atcaggttcc ggaagaattc    720

aaaccctggg cgtacctggc caccggcgac agctgtatta tcgaccctcg tggagaagtc    780

attgccgggc cggcccgcgg cgaaaccatc ctgaccgcgg aaatcgacac cgaacagatc    840

tacgccgcca aagccgcttt tgacgcggca gggcactatg cacggcccga catctttcaa    900

ctgcatgtaa accgctcctc ccaccggccc ctggttgaat cttccatcgg cttgaaaccg    960

gccaagaccg gagtgcagca aaagcttgat ccgcctggct tcgcgccttc gcaagaatga   1020

<210> 24
<211> 339
<212> PRT
<213> Unknown

<220> 
<223> Obtained from environmental sample

<220> 
<221> DOMAIN
<222> (8)...(192)
<223> Carbon-nitrogen hydrolase

<220> 
<221> SITE
<222> (308)...(311)
<223> N-glycosylation site. Prosite id = PS00001

<400> 24
Met Thr Gln Tyr Ser Lys Phe Lys Val Ala Ala Ile Gln Ala Ala Pro 
1               5                   10                  15      


Val Tyr Phe Asn Pro Glu Ala Ser Thr Ala Lys Ala Cys His Leu Ile 
            20                  25                  30          


Glu Ser Ala Gly Gln Lys Gly Val Glu Leu Ala Ala Phe Gly Glu Thr 
        35                  40                  45              


Trp Leu Pro Gly Tyr Pro Phe Phe Val Trp Gly Ala Gly Arg Gly Arg 
    50                  55                  60                  


Ala Ala Trp Lys Ala Gln Ala Ala Tyr Leu Ser Ala Ala Val Glu Ile 
65                  70                  75                  80  


Pro Ser Pro Val Thr Glu Gln Leu Cys Ala Ala Ala Arg Arg Ala Ser 
                85                  90                  95      


Ile Asp Val Val Ile Gly Val Ala Glu Arg Asp Ser Leu Ser Arg Gly 
            100                 105                 110         


Thr Val Tyr Cys Thr Leu Leu Phe Ile Ser Arg Glu Gly Ser Ile Leu 
        115                 120                 125             


Gly Arg His Arg Lys Leu Arg Pro Thr His Lys Glu Arg Thr Ala Trp 
    130                 135                 140                 


Gly Asp Gly Asp Ala Val Gly Leu Ser Val Tyr Glu Arg Pro Tyr Gly 
145                 150                 155                 160 


Arg Ile Ser Gly Leu Asn Cys Trp Glu His Asn Met Val Leu Pro Gly 
                165                 170                 175     


Tyr Ala Leu Met Thr Gln Gly Thr Gln Ile His Val Ala Ala Trp Pro 
            180                 185                 190         


Gly Thr Glu Ala Pro Ala Pro Pro Ala Pro Ile Pro Met Trp Glu Arg 
        195                 200                 205             


Gln Leu Leu Leu Ser Arg Ala Phe Ala Ser Gln Ala Ala Ala Tyr Val 
    210                 215                 220                 


Ile Gln Ala Gly Gly Leu Leu Ser Glu Asp Gln Val Pro Glu Glu Phe 
225                 230                 235                 240 


Lys Pro Trp Ala Tyr Leu Ala Thr Gly Asp Ser Cys Ile Ile Asp Pro 
                245                 250                 255     


Arg Gly Glu Val Ile Ala Gly Pro Ala Arg Gly Glu Thr Ile Leu Thr 
            260                 265                 270         


Ala Glu Ile Asp Thr Glu Gln Ile Tyr Ala Ala Lys Ala Ala Phe Asp 
        275                 280                 285             


Ala Ala Gly His Tyr Ala Arg Pro Asp Ile Phe Gln Leu His Val Asn 
    290                 295                 300                 


Arg Ser Ser His Arg Pro Leu Val Glu Ser Ser Ile Gly Leu Lys Pro 
305                 310                 315                 320 


Ala Lys Thr Gly Val Gln Gln Lys Leu Asp Pro Pro Gly Phe Ala Pro 
                325                 330                 335     


Ser Gln Glu 
            


<210> 25
<211> 1011
<212> DNA
<213> Unknown

<220> 
<223> Obtained from environmental sample

<400> 25
atggctgaca acgaaacggt aaaagtagcc gcggcgcagg taacgccggt cttcctggac     60

cgcgaggcga ccatccaaaa ggcgtgtgac gtgatcgcgg aggcggcccg ggagggcgcc    120

cgcctggtcg tcttcccgga ggcgttcgtg ccggcgtacc ccgactggat ctggcgcgtg    180

ccggcgggcg agatgggcgt gatggcagag ctccacggcg agctgctggc gaactccgtc    240

gagataccgg ggccggcgac ggaggcgctg tgcagggcgg cccggcagca caacatctgc    300

gtcgtgatgg gcgtcaacga gcgcaacgtg gaggccagcg gcggcaccct ctacaacacg    360

ctcgtctaca tagacgacaa gggtgagctg ctgggcaagc accgcaagct ggtgccgacg    420

ggcggagagc ggctggtctg ggcgcagggc gacggcagca cgctggaggt gtacgacctg    480

ccgttcggca ggctgggcgg actgatctgc tgggagaact acatgccgcc cgcccgctac    540

gccatgtacg cgtggggcac gcagatctac gtcgccgcta catgggacca gggagagccg    600

tggctatcca cgctgcggca catcggcaag gagggacgcg tcttcgtcat cggcgtctgc    660

atgcccttcc gctccaggga catcccggac cgcttcgagt tcaagcaccg cttctatgac    720

ccggagcgcg actggctgaa caacggtgac agcgccatcg tcagcccgga gggtcacttc    780

atcgcgggac cgatgcacag ggaggaggga atcctctacg cggagctgga ccgtcgccag    840

atgaccgggc cgcgctggat gctggatgtg gcgggacatt acggcaggcc ggacgtcttc    900

cacttgacag tctcccgaga gaggcacgat atgctagagg tgcgcgacgg cgcgcacgcg    960

ggggcggagc cggacgtagc ggaagagccg ggcaaagaag cccggaagta g            1011

<210> 26
<211> 336
<212> PRT
<213> Unknown

<220> 
<223> Obtained from environmental sample

<220> 
<221> DOMAIN
<222> (8)...(195)
<223> Carbon-nitrogen hydrolase

<220> 
<221> SITE
<222> (4)...(7)
<223> N-glycosylation site. Prosite id = PS00001

<400> 26
Met Ala Asp Asn Glu Thr Val Lys Val Ala Ala Ala Gln Val Thr Pro 
1               5                   10                  15      


Val Phe Leu Asp Arg Glu Ala Thr Ile Gln Lys Ala Cys Asp Val Ile 
            20                  25                  30          


Ala Glu Ala Ala Arg Glu Gly Ala Arg Leu Val Val Phe Pro Glu Ala 
        35                  40                  45              


Phe Val Pro Ala Tyr Pro Asp Trp Ile Trp Arg Val Pro Ala Gly Glu 
    50                  55                  60                  


Met Gly Val Met Ala Glu Leu His Gly Glu Leu Leu Ala Asn Ser Val 
65                  70                  75                  80  


Glu Ile Pro Gly Pro Ala Thr Glu Ala Leu Cys Arg Ala Ala Arg Gln 
                85                  90                  95      


His Asn Ile Cys Val Val Met Gly Val Asn Glu Arg Asn Val Glu Ala 
            100                 105                 110         


Ser Gly Gly Thr Leu Tyr Asn Thr Leu Val Tyr Ile Asp Asp Lys Gly 
        115                 120                 125             


Glu Leu Leu Gly Lys His Arg Lys Leu Val Pro Thr Gly Gly Glu Arg 
    130                 135                 140                 


Leu Val Trp Ala Gln Gly Asp Gly Ser Thr Leu Glu Val Tyr Asp Leu 
145                 150                 155                 160 


Pro Phe Gly Arg Leu Gly Gly Leu Ile Cys Trp Glu Asn Tyr Met Pro 
                165                 170                 175     


Pro Ala Arg Tyr Ala Met Tyr Ala Trp Gly Thr Gln Ile Tyr Val Ala 
            180                 185                 190         


Ala Thr Trp Asp Gln Gly Glu Pro Trp Leu Ser Thr Leu Arg His Ile 
        195                 200                 205             


Gly Lys Glu Gly Arg Val Phe Val Ile Gly Val Cys Met Pro Phe Arg 
    210                 215                 220                 


Ser Arg Asp Ile Pro Asp Arg Phe Glu Phe Lys His Arg Phe Tyr Asp 
225                 230                 235                 240 


Pro Glu Arg Asp Trp Leu Asn Asn Gly Asp Ser Ala Ile Val Ser Pro 
                245                 250                 255     


Glu Gly His Phe Ile Ala Gly Pro Met His Arg Glu Glu Gly Ile Leu 
            260                 265                 270         


Tyr Ala Glu Leu Asp Arg Arg Gln Met Thr Gly Pro Arg Trp Met Leu 
        275                 280                 285             


Asp Val Ala Gly His Tyr Gly Arg Pro Asp Val Phe His Leu Thr Val 
    290                 295                 300                 


Ser Arg Glu Arg His Asp Met Leu Glu Val Arg Asp Gly Ala His Ala 
305                 310                 315                 320 


Gly Ala Glu Pro Asp Val Ala Glu Glu Pro Gly Lys Glu Ala Arg Lys 
                325                 330                 335     


<210> 27
<211> 1011
<212> DNA
<213> Unknown

<220> 
<223> Obtained from environmental sample

<400> 27
atggctgaca acgaaacggt gaaagtggcc gcggcccagg tgacgcccgt cttcctggac     60

cgcgacgcca ccatccagaa ggcgtgcgac gtgatcgcgg aggcggcccg ggagggcgcc    120

cgcctgatcg tcttcccgga ggcgttcgtg cccgcgtacc ccgactggat ctggcgcgtc    180

ccggcgggcg aggtaaccgt catggcagag ctccacggcg agctgctggc gaactccgtc    240

gagataccgg ggccggcgac cgacgcgctg tgcagggcgg cccggcagaa caacatctgc    300

gtcgtgatgg gcgtcaacga gcgcaacgtg gaggccagcg gcggcagcct ctacaacacc    360

ctcgtctaca tagacgacaa agggaacctg ctgggcaagc accggaagct ggtgcccacc    420

ggcggggagc ggctgatctg ggcgcagggc gatgggagca cgctggaggt gtacgacctg    480

ccgttcggca ggctgggcgg gctcatctgc tgggagaact acatgccgcc cgcccgctac    540

gcgatgtacg cgtggggcac gcaggtctac gtggccgcca cctgggaccg gggcgagccg    600

tggctttcca ccctgcgaca catcggcaag gagggacggg tgttcgtcat cggcgtctgc    660

atgccgttcc ggaccaggga catcccggat cggttcgagt tcaagcaccg cttctatgac    720

ccggagcgcg actggctgaa cgacggcgac agcgcgatcg tcaacccgga gggtcacttc    780

atcgcggggc cgatgcacaa ggaggagggc atcctctacg cggagctgga ccgtcgccag    840

atgaccgggc cgcggtggat gctggacgtg gccggccact acggcaggcc ggacgtcttc    900

cacttgacag tctcacggga gaggcacgat atgctagagg tgcgagacgg tgcacacgct    960

ggggtggagc cggacatagc ggaagagccg ggcgcggaag cccggaagta g            1011

<210> 28
<211> 336
<212> PRT
<213> Unknown

<220> 
<223> Obtained from environmental sample

<220> 
<221> DOMAIN
<222> (8)...(195)
<223> Carbon-nitrogen hydrolase

<220> 
<221> SITE
<222> (4)...(7)
<223> N-glycosylation site. Prosite id = PS00001

<400> 28
Met Ala Asp Asn Glu Thr Val Lys Val Ala Ala Ala Gln Val Thr Pro 
1               5                   10                  15      


Val Phe Leu Asp Arg Asp Ala Thr Ile Gln Lys Ala Cys Asp Val Ile 
            20                  25                  30          


Ala Glu Ala Ala Arg Glu Gly Ala Arg Leu Ile Val Phe Pro Glu Ala 
        35                  40                  45              


Phe Val Pro Ala Tyr Pro Asp Trp Ile Trp Arg Val Pro Ala Gly Glu 
    50                  55                  60                  


Val Thr Val Met Ala Glu Leu His Gly Glu Leu Leu Ala Asn Ser Val 
65                  70                  75                  80  


Glu Ile Pro Gly Pro Ala Thr Asp Ala Leu Cys Arg Ala Ala Arg Gln 
                85                  90                  95      


Asn Asn Ile Cys Val Val Met Gly Val Asn Glu Arg Asn Val Glu Ala 
            100                 105                 110         


Ser Gly Gly Ser Leu Tyr Asn Thr Leu Val Tyr Ile Asp Asp Lys Gly 
        115                 120                 125             


Asn Leu Leu Gly Lys His Arg Lys Leu Val Pro Thr Gly Gly Glu Arg 
    130                 135                 140                 


Leu Ile Trp Ala Gln Gly Asp Gly Ser Thr Leu Glu Val Tyr Asp Leu 
145                 150                 155                 160 


Pro Phe Gly Arg Leu Gly Gly Leu Ile Cys Trp Glu Asn Tyr Met Pro 
                165                 170                 175     


Pro Ala Arg Tyr Ala Met Tyr Ala Trp Gly Thr Gln Val Tyr Val Ala 
            180                 185                 190         


Ala Thr Trp Asp Arg Gly Glu Pro Trp Leu Ser Thr Leu Arg His Ile 
        195                 200                 205             


Gly Lys Glu Gly Arg Val Phe Val Ile Gly Val Cys Met Pro Phe Arg 
    210                 215                 220                 


Thr Arg Asp Ile Pro Asp Arg Phe Glu Phe Lys His Arg Phe Tyr Asp 
225                 230                 235                 240 


Pro Glu Arg Asp Trp Leu Asn Asp Gly Asp Ser Ala Ile Val Asn Pro 
                245                 250                 255     


Glu Gly His Phe Ile Ala Gly Pro Met His Lys Glu Glu Gly Ile Leu 
            260                 265                 270         


Tyr Ala Glu Leu Asp Arg Arg Gln Met Thr Gly Pro Arg Trp Met Leu 
        275                 280                 285             


Asp Val Ala Gly His Tyr Gly Arg Pro Asp Val Phe His Leu Thr Val 
    290                 295                 300                 


Ser Arg Glu Arg His Asp Met Leu Glu Val Arg Asp Gly Ala His Ala 
305                 310                 315                 320 


Gly Val Glu Pro Asp Ile Ala Glu Glu Pro Gly Ala Glu Ala Arg Lys 
                325                 330                 335     


<210> 29
<211> 1014
<212> DNA
<213> Unknown

<220> 
<223> Obtained from environmental sample

<400> 29
atgaaagaag ctatcaaggt cgcctgcgtg caagccgccc cgatctacat ggatttggag     60

gcgacggtgg acaaaaccat tgagttgatg gaagaagcag cacgtaataa tgctcgtctg    120

atcgcctttc cggaaacttg gattccaggc tacccatggt ttctttggct tgactcacca    180

gcatgggcaa tgcaatttgt acgccaatac catgagaact cattggagtt ggatggccct    240

caagctaagc gcatttcaga tgcagccaag cggttgggaa tcatggtcac cctggggatg    300

agtgaacggg tcggtggcac cctttacatc agtcagtggt tcataggcga taatggtgac    360

accattgggg cccggcgaaa gttgaaacct acttttgttg aacgtacttt gttcggcgaa    420

ggggatggtt catcgctagc ggttttcgag acgtctgttg gaaggctggg tggcttatgc    480

tgttgggagc accttcaacc gctaacaaaa tacgctttgt atgcacaaaa tgaagagatt    540

cattgtgcgg cttggccgag ctttagcctt tatcctaatg cggcgaaagc cctggggcct    600

gatgtcaatg tagcggcctc tcgaatctat gccgttgaag ggcaatgctt cgtactagcg    660

tcgtgtgcgc tcgtttcaca atccatgatc gatatgcttt gtacagatga cgaaaagcat    720

gcgttgcttc tggctggtgg tggacactca cgtatcatag ggcctgatgg tggtgacttg    780

gtcgcgcctc ttgccgaaaa tgaagagggt attctctacg caaaccttga tcctggagta    840

cgcatccttg ctaaaatggc ggcagaccct gctggtcatt attcccgtcc cgacattact    900

cgcttgctaa tagatcgcag ccctaaatta ccggtagttg aaattgaagg tgatcttcgt    960

ccttacgctt tgggtaaagc gtctgagacg ggtgcgcaac tcgaagaaat ttga         1014

<210> 30
<211> 337
<212> PRT
<213> Unknown

<220> 
<223> Obtained from environmental sample

<220> 
<221> DOMAIN
<222> (6)...(186)
<223> Carbon-nitrogen hydrolase

<220> 
<221> SITE
<222> (159)...(172)
<223> Nitrilases / cyanide hydratase active site signature. Prosite id = PS00921

<400> 30
Met Lys Glu Ala Ile Lys Val Ala Cys Val Gln Ala Ala Pro Ile Tyr 
1               5                   10                  15      


Met Asp Leu Glu Ala Thr Val Asp Lys Thr Ile Glu Leu Met Glu Glu 
            20                  25                  30          


Ala Ala Arg Asn Asn Ala Arg Leu Ile Ala Phe Pro Glu Thr Trp Ile 
        35                  40                  45              


Pro Gly Tyr Pro Trp Phe Leu Trp Leu Asp Ser Pro Ala Trp Ala Met 
    50                  55                  60                  


Gln Phe Val Arg Gln Tyr His Glu Asn Ser Leu Glu Leu Asp Gly Pro 
65                  70                  75                  80  


Gln Ala Lys Arg Ile Ser Asp Ala Ala Lys Arg Leu Gly Ile Met Val 
                85                  90                  95      


Thr Leu Gly Met Ser Glu Arg Val Gly Gly Thr Leu Tyr Ile Ser Gln 
            100                 105                 110         


Trp Phe Ile Gly Asp Asn Gly Asp Thr Ile Gly Ala Arg Arg Lys Leu 
        115                 120                 125             


Lys Pro Thr Phe Val Glu Arg Thr Leu Phe Gly Glu Gly Asp Gly Ser 
    130                 135                 140                 


Ser Leu Ala Val Phe Glu Thr Ser Val Gly Arg Leu Gly Gly Leu Cys 
145                 150                 155                 160 


Cys Trp Glu His Leu Gln Pro Leu Thr Lys Tyr Ala Leu Tyr Ala Gln 
                165                 170                 175     


Asn Glu Glu Ile His Cys Ala Ala Trp Pro Ser Phe Ser Leu Tyr Pro 
            180                 185                 190         


Asn Ala Ala Lys Ala Leu Gly Pro Asp Val Asn Val Ala Ala Ser Arg 
        195                 200                 205             


Ile Tyr Ala Val Glu Gly Gln Cys Phe Val Leu Ala Ser Cys Ala Leu 
    210                 215                 220                 


Val Ser Gln Ser Met Ile Asp Met Leu Cys Thr Asp Asp Glu Lys His 
225                 230                 235                 240 


Ala Leu Leu Leu Ala Gly Gly Gly His Ser Arg Ile Ile Gly Pro Asp 
                245                 250                 255     


Gly Gly Asp Leu Val Ala Pro Leu Ala Glu Asn Glu Glu Gly Ile Leu 
            260                 265                 270         


Tyr Ala Asn Leu Asp Pro Gly Val Arg Ile Leu Ala Lys Met Ala Ala 
        275                 280                 285             


Asp Pro Ala Gly His Tyr Ser Arg Pro Asp Ile Thr Arg Leu Leu Ile 
    290                 295                 300                 


Asp Arg Ser Pro Lys Leu Pro Val Val Glu Ile Glu Gly Asp Leu Arg 
305                 310                 315                 320 


Pro Tyr Ala Leu Gly Lys Ala Ser Glu Thr Gly Ala Gln Leu Glu Glu 
                325                 330                 335     


Ile 
    


<210> 31
<211> 1014
<212> DNA
<213> Unknown

<220> 
<223> Obtained from environmental sample

<400> 31
atgtccgaaa ctgcattcaa gatcgccgtc gtgcaggcgg cccctgtctt tctggacgcc     60

gcggcttcca cggaaaaggc catcagcctg atcgccgagg cgggtgcgaa cggggcgaag    120

ctgttggcct ttcccgaagt gttcataccc ggctacccgt ggtggctctg gctcggtacg    180

ccggcttggg gaatgcagtt cgtgtcgcgt taccacgcca actcattgcg cgcggatgga    240

ccggaactcg cctccatagc ggcggctgct gccaaggcga acatcaatgt cgtgatgggc    300

ttctccgaaa tcgatggcgg gtcgctctac atcagccagg cgttgatcag cgacaaaggc    360

gagatggtct tcaagcgccg caagctcaag cccactcacg tggagcgcac cctgttcgga    420

gagggcgatg gctccgattt tcaggtggtc gacaccagcg tcgggcgcct gggtgcactc    480

tgctgtgccg agcatatcca gccgctgtcg aagtacgcga tgtactcgat gcacgagcaa    540

gtacatgtcg cttcgtggcc gtcgttcact ctctaccgtg gcactgccta tgcgctggga    600

catgaggtga atctttccgc gagccagatc tacgcactgg aaggtggttg cttcgtcttg    660

catgcgagtg ccatcaccgg ccaggacatg ttcgacgtca tgtgcgacac gcctgaaaag    720

gcacaccttc tcaattcaga cggcggcaag cctggtggtg ggtactcgat gatttttggc    780

cccgatggcc aaccgttggt ggagcacctg ccgcaggatc aggaaggcat tctttacgcg    840

gacatagacc tgtccacgat cgccgtcgca aaggcggcct acgacccgtc gggacactac    900

gcgcgtggcg acgtggtccg gctgatggtc aatcgcagtc cgcgccgcac cagcatcaac    960

tttggagaag gcgtgacaga aggtgcgcag tggaccgagg cgaaagtcga ttga         1014

<210> 32
<211> 337
<212> PRT
<213> Unknown

<220> 
<223> Obtained from environmental sample

<220> 
<221> DOMAIN
<222> (7)...(187)
<223> Carbon-nitrogen hydrolase

<220> 
<221> SITE
<222> (38)...(53)
<223> Nitrilases / cyanide hydratase signature 1. Prosite id = PS00920

<220> 
<221> SITE
<222> (160)...(173)
<223> Nitrilases / cyanide hydratase active site signature. Prosite id = PS00921

<220> 
<221> SITE
<222> (207)...(210)
<223> N-glycosylation site. Prosite id = PS00001

<400> 32
Met Ser Glu Thr Ala Phe Lys Ile Ala Val Val Gln Ala Ala Pro Val 
1               5                   10                  15      


Phe Leu Asp Ala Ala Ala Ser Thr Glu Lys Ala Ile Ser Leu Ile Ala 
            20                  25                  30          


Glu Ala Gly Ala Asn Gly Ala Lys Leu Leu Ala Phe Pro Glu Val Phe 
        35                  40                  45              


Ile Pro Gly Tyr Pro Trp Trp Leu Trp Leu Gly Thr Pro Ala Trp Gly 
    50                  55                  60                  


Met Gln Phe Val Ser Arg Tyr His Ala Asn Ser Leu Arg Ala Asp Gly 
65                  70                  75                  80  


Pro Glu Leu Ala Ser Ile Ala Ala Ala Ala Ala Lys Ala Asn Ile Asn 
                85                  90                  95      


Val Val Met Gly Phe Ser Glu Ile Asp Gly Gly Ser Leu Tyr Ile Ser 
            100                 105                 110         


Gln Ala Leu Ile Ser Asp Lys Gly Glu Met Val Phe Lys Arg Arg Lys 
        115                 120                 125             


Leu Lys Pro Thr His Val Glu Arg Thr Leu Phe Gly Glu Gly Asp Gly 
    130                 135                 140                 


Ser Asp Phe Gln Val Val Asp Thr Ser Val Gly Arg Leu Gly Ala Leu 
145                 150                 155                 160 


Cys Cys Ala Glu His Ile Gln Pro Leu Ser Lys Tyr Ala Met Tyr Ser 
                165                 170                 175     


Met His Glu Gln Val His Val Ala Ser Trp Pro Ser Phe Thr Leu Tyr 
            180                 185                 190         


Arg Gly Thr Ala Tyr Ala Leu Gly His Glu Val Asn Leu Ser Ala Ser 
        195                 200                 205             


Gln Ile Tyr Ala Leu Glu Gly Gly Cys Phe Val Leu His Ala Ser Ala 
    210                 215                 220                 


Ile Thr Gly Gln Asp Met Phe Asp Val Met Cys Asp Thr Pro Glu Lys 
225                 230                 235                 240 


Ala His Leu Leu Asn Ser Asp Gly Gly Lys Pro Gly Gly Gly Tyr Ser 
                245                 250                 255     


Met Ile Phe Gly Pro Asp Gly Gln Pro Leu Val Glu His Leu Pro Gln 
            260                 265                 270         


Asp Gln Glu Gly Ile Leu Tyr Ala Asp Ile Asp Leu Ser Thr Ile Ala 
        275                 280                 285             


Val Ala Lys Ala Ala Tyr Asp Pro Ser Gly His Tyr Ala Arg Gly Asp 
    290                 295                 300                 


Val Val Arg Leu Met Val Asn Arg Ser Pro Arg Arg Thr Ser Ile Asn 
305                 310                 315                 320 


Phe Gly Glu Gly Val Thr Glu Gly Ala Gln Trp Thr Glu Ala Lys Val 
                325                 330                 335     


Asp 
    



