
                                SEQUENCE LISTING

<110> Coleman, Russell J.
      Retallack, Diane M.
      Schneider, Jane C.
      Ramseier, Thomas M.
      Hershberger, Charles D.
      Lee, Stacey L.
      Resnick, Sol M.
      

<120> BACTERIAL LEADER SEQUENCES FOR INCREASED
  EXPRESSION

<130> 43292/339376

<160> 50

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 72
<212> DNA
<213> Artificial Sequence

<220> 
<223> mutant phosphate binding protein leader sequence
      (pbp*)

<221> CDS            
<222> (1)...(72)

<400> 1
atg aaa ctg aaa cgt ttg atg gcg gca atg act ttt gtc gct gct ggc   48
Met Lys Leu Lys Arg Leu Met Ala Ala Met Thr Phe Val Ala Ala Gly
 1               5                   10                  15
 
gtt gcg acc gtc aac gcg gtg gcc                                   72
Val Ala Thr Val Asn Ala Val Ala
             20
 

<210> 2
<211> 24
<212> PRT
<213> Artificial Sequence

<220> 
<223> mutant phosphate binding protein leader sequence
      (pbp*)

<400> 2
Met Lys Leu Lys Arg Leu Met Ala Ala Met Thr Phe Val Ala Ala Gly
 1               5                  10                  15      
Val Ala Thr Val Asn Ala Val Ala
            20                  


<210> 3
<211> 66
<212> DNA
<213> Pseudomonas fluorescens

<220> 
<221> CDS            
<222> (1)...(66)

<400> 3
atg cgt aat ctg atc ctc agc gcc gct ctc gtc act gcc agc ctc ttc   48
Met Arg Asn Leu Ile Leu Ser Ala Ala Leu Val Thr Ala Ser Leu Phe
 1               5                   10                  15
 
ggc atg acc gca caa gct                                           66
Gly Met Thr Ala Gln Ala
             20
 

<210> 4
<211> 22
<212> PRT
<213> Pseudomonas fluorescens

<400> 4
Met Arg Asn Leu Ile Leu Ser Ala Ala Leu Val Thr Ala Ser Leu Phe
 1               5                  10                  15      
Gly Met Thr Ala Gln Ala
            20          


<210> 5
<211> 63
<212> DNA
<213> Pseudomonas fluorescens

<220> 
<221> CDS            
<222> (1)...(63)

<400> 5
atg cgc ttg acc cag att att gcc gcc gca gcc att gcg ttg gtt tcc   48
Met Arg Leu Thr Gln Ile Ile Ala Ala Ala Ala Ile Ala Leu Val Ser
 1               5                   10                  15
 
acc ttt gcg ctc gcc                                               63
Thr Phe Ala Leu Ala
             20
 

<210> 6
<211> 21
<212> PRT
<213> Pseudomonas fluorescens

<400> 6
Met Arg Leu Thr Gln Ile Ile Ala Ala Ala Ala Ile Ala Leu Val Ser
 1               5                  10                  15      
Thr Phe Ala Leu Ala
            20      


<210> 7
<211> 99
<212> DNA
<213> Bacillus coagulans

<220> 
<221> CDS            
<222> (1)...(99)

<400> 7
atg agc aca cga atc ccc cgc cga caa tgg ctg aaa ggc gcc tcg ggc   48
Met Ser Thr Arg Ile Pro Arg Arg Gln Trp Leu Lys Gly Ala Ser Gly
 1               5                   10                  15
 
ctg ctg gcc gcc gcg agc ctg ggc cgg ttg gcc aac cgc gag gcg cgc   96
Leu Leu Ala Ala Ala Ser Leu Gly Arg Leu Ala Asn Arg Glu Ala Arg
             20                  25                  30
 
gcc                                                               99
Ala

 

<210> 8
<211> 33
<212> PRT
<213> Bacillus coagulans

<400> 8
Met Ser Thr Arg Ile Pro Arg Arg Gln Trp Leu Lys Gly Ala Ser Gly
 1               5                  10                  15      
Leu Leu Ala Ala Ala Ser Leu Gly Arg Leu Ala Asn Arg Glu Ala Arg
            20                  25                  30          
Ala
    


<210> 9
<211> 75
<212> DNA
<213> Pseudomonas fluorescens

<220> 
<221> CDS            
<222> (1)...(75)

<400> 9
atg tcg tgc aca cgt gca ttc aaa cca ctg ctg ctg atc ggc ctg gcc   48
Met Ser Cys Thr Arg Ala Phe Lys Pro Leu Leu Leu Ile Gly Leu Ala
 1               5                   10                  15
 
aca ctg atg tgt tcc cat gca ttc gct                               75
Thr Leu Met Cys Ser His Ala Phe Ala
             20                  25
 

<210> 10
<211> 25
<212> PRT
<213> Pseudomonas fluorescens

<400> 10
Met Ser Cys Thr Arg Ala Phe Lys Pro Leu Leu Leu Ile Gly Leu Ala
 1               5                  10                  15      
Thr Leu Met Cys Ser His Ala Phe Ala
            20                  25  


<210> 11
<211> 72
<212> DNA
<213> Pseudomonas fluorescens

<220> 
<221> CDS            
<222> (1)...(72)

<400> 11
atg ctt ttt cgc aca tta ctg gcg agc ctt acc ttt gct gtc atc gcc   48
Met Leu Phe Arg Thr Leu Leu Ala Ser Leu Thr Phe Ala Val Ile Ala
 1               5                   10                  15
 
ggc tta ccg tcc acg gcc cac gcg                                   72
Gly Leu Pro Ser Thr Ala His Ala
             20
 

<210> 12
<211> 24
<212> PRT
<213> Pseudomonas fluorescens

<400> 12
Met Leu Phe Arg Thr Leu Leu Ala Ser Leu Thr Phe Ala Val Ile Ala
 1               5                  10                  15      
Gly Leu Pro Ser Thr Ala His Ala
            20                  


<210> 13
<211> 69
<212> DNA
<213> Pseudomonas fluorescens

<220> 
<221> CDS            
<222> (1)...(69)

<400> 13
atg ccg cct cgt tct atc gcc gca tgt ctg ggg ctg ctg ggc ttg ctc   48
Met Pro Pro Arg Ser Ile Ala Ala Cys Leu Gly Leu Leu Gly Leu Leu
 1               5                   10                  15
 
atg gct acc cag gcc gcc gcc                                       69
Met Ala Thr Gln Ala Ala Ala
             20
 

<210> 14
<211> 23
<212> PRT
<213> Pseudomonas fluorescens

<400> 14
Met Pro Pro Arg Ser Ile Ala Ala Cys Leu Gly Leu Leu Gly Leu Leu
 1               5                  10                  15      
Met Ala Thr Gln Ala Ala Ala
            20              


<210> 15
<211> 63
<212> DNA
<213> Pseudomonas fluorescens

<220> 
<221> CDS            
<222> (1)...(63)

<400> 15
atg cgc ctc gct gcc cta ccg cta ttg ctt gcc cct ctc ttt att gcg   48
Met Arg Leu Ala Ala Leu Pro Leu Leu Leu Ala Pro Leu Phe Ile Ala
 1               5                   10                  15
 
ccg atg gcc gtt gcg                                               63
Pro Met Ala Val Ala
             20
 

<210> 16
<211> 21
<212> PRT
<213> Pseudomonas fluorescens

<400> 16
Met Arg Leu Ala Ala Leu Pro Leu Leu Leu Ala Pro Leu Phe Ile Ala
 1               5                  10                  15      
Pro Met Ala Val Ala
            20      


<210> 17
<211> 63
<212> DNA
<213> Pseudomonas fluorescens

<220> 
<221> CDS            
<222> (1)...(63)

<400> 17
atg aag ttc aaa cag ctg atg gcg atg gcg ctt ttg ttg gcc ttg agc   48
Met Lys Phe Lys Gln Leu Met Ala Met Ala Leu Leu Leu Ala Leu Ser
 1               5                   10                  15
 
gct gtg gcc cag gcc                                               63
Ala Val Ala Gln Ala
             20
 

<210> 18
<211> 21
<212> PRT
<213> Pseudomonas fluorescens

<400> 18
Met Lys Phe Lys Gln Leu Met Ala Met Ala Leu Leu Leu Ala Leu Ser
 1               5                  10                  15      
Ala Val Ala Gln Ala
            20      


<210> 19
<211> 63
<212> DNA
<213> Pseudomonas fluorescens

<220> 
<221> CDS            
<222> (1)...(63)

<400> 19
atg aat aga tct tcc gcg ttg ctc ctc gct ttt gtc ttc ctc agc ggc   48
Met Asn Arg Ser Ser Ala Leu Leu Leu Ala Phe Val Phe Leu Ser Gly
 1               5                   10                  15
 
tgc cag gcc atg gcc                                               63
Cys Gln Ala Met Ala
             20
 

<210> 20
<211> 21
<212> PRT
<213> Pseudomonas fluorescens

<400> 20
Met Asn Arg Ser Ser Ala Leu Leu Leu Ala Phe Val Phe Leu Ser Gly
 1               5                  10                  15      
Cys Gln Ala Met Ala
            20      


<210> 21
<211> 99
<212> DNA
<213> Pseudomonas fluorescens

<220> 
<221> CDS            
<222> (1)...(99)

<400> 21
atg caa aac cgc act gtg gaa atc ggt gtc ggc ctt ttc ttg ctg gct   48
Met Gln Asn Arg Thr Val Glu Ile Gly Val Gly Leu Phe Leu Leu Ala
 1               5                   10                  15
 
ggc atc ctg gct tta ctg ttg ttg gcc ctg cga gtc agc ggc ctt tcg   96
Gly Ile Leu Ala Leu Leu Leu Leu Ala Leu Arg Val Ser Gly Leu Ser
             20                  25                  30
 
gcc                                                               99
Ala

 

<210> 22
<211> 33
<212> PRT
<213> Pseudomonas fluorescens

<400> 22
Met Gln Asn Arg Thr Val Glu Ile Gly Val Gly Leu Phe Leu Leu Ala
 1               5                  10                  15      
Gly Ile Leu Ala Leu Leu Leu Leu Ala Leu Arg Val Ser Gly Leu Ser
            20                  25                  30          
Ala
    


<210> 23
<211> 117
<212> DNA
<213> Pseudomonas fluorescens

<220> 
<221> CDS            
<222> (1)...(117)

<400> 23
atg tct ctt cgt aat atg aat atc gcc ccg agg gcc ttc ctc ggc ttc   48
Met Ser Leu Arg Asn Met Asn Ile Ala Pro Arg Ala Phe Leu Gly Phe
 1               5                   10                  15
 
gcg ttt att ggc gcc ttg atg ttg ttg ctc ggt gtg ttc gcg ctg aac   96
Ala Phe Ile Gly Ala Leu Met Leu Leu Leu Gly Val Phe Ala Leu Asn
             20                  25                  30
 
cag atg agc aaa att cgt gcg                                       117
Gln Met Ser Lys Ile Arg Ala
         35
 

<210> 24
<211> 39
<212> PRT
<213> Pseudomonas fluorescens

<400> 24
Met Ser Leu Arg Asn Met Asn Ile Ala Pro Arg Ala Phe Leu Gly Phe
 1               5                  10                  15      
Ala Phe Ile Gly Ala Leu Met Leu Leu Leu Gly Val Phe Ala Leu Asn
            20                  25                  30          
Gln Met Ser Lys Ile Arg Ala
        35                  


<210> 25
<211> 30
<212> DNA
<213> Artificial Sequence

<220> 
<223> oligonucleotide primer

<400> 25
aattactagt aggaggtaca ttatgcgctt                                  30

<210> 26
<211> 30
<212> DNA
<213> Artificial Sequence

<220> 
<223> oligonucleotide primer

<400> 26
tatactcgag ttatttaacc tgtttcagta                                  30

<210> 27
<211> 5
<212> PRT
<213> Artificial Sequence

<220> 
<223> First 5 amino acids of the predicted protein
      sequence for the processed form of dsbC-Skp

<400> 27
Ala Asp Lys Ile Ala
 1               5  


<210> 28
<211> 10
<212> PRT
<213> Artificial Sequence

<220> 
<223> First 10 amino acids of the predicted protein
      sequence for the unprocessed form of dsbC-Skp
      
      

<400> 28
Met Arg Leu Thr Gln Ile Ile Ala Ala Ala
 1               5                  10  


<210> 29
<211> 10
<212> PRT
<213> Artificial Sequence

<220> 
<223> First 10 amino acids of the predicted protein
      sequence for the processed form of dsbC-Skp
      
      
      

<400> 29
Ala Asp Lys Ile Ala Ile Val Asn Met Gly
 1               5                  10  


<210> 30
<211> 63
<212> DNA
<213> Pseudomonas fluorescens

<220> 
<221> CDS            
<222> (1)...(63)

<400> 30
atg aag aag tcc acc ttg gct gtg gct gta acg ttg ggc gca atc gcc   48
Met Lys Lys Ser Thr Leu Ala Val Ala Val Thr Leu Gly Ala Ile Ala
 1               5                   10                  15
 
cag caa gca ggc gct                                               63
Gln Gln Ala Gly Ala
             20
 

<210> 31
<211> 21
<212> PRT
<213> Pseudomonas fluorescens

<400> 31
Met Lys Lys Ser Thr Leu Ala Val Ala Val Thr Leu Gly Ala Ile Ala
 1               5                  10                  15      
Gln Gln Ala Gly Ala
            20      


<210> 32
<211> 72
<212> DNA
<213> Pseudomonas fluorescens

<220> 
<221> CDS            
<222> (1)...(72)

<400> 32
atg aaa ctg aaa aac acc ttg ggc ttg gcc att ggt tct ctt att gcc   48
Met Lys Leu Lys Asn Thr Leu Gly Leu Ala Ile Gly Ser Leu Ile Ala
 1               5                   10                  15
 
gct act tct ttc ggc gtt ctg gca                                   72
Ala Thr Ser Phe Gly Val Leu Ala
             20
 

<210> 33
<211> 24
<212> PRT
<213> Pseudomonas fluorescens

<400> 33
Met Lys Leu Lys Asn Thr Leu Gly Leu Ala Ile Gly Ser Leu Ile Ala
 1               5                  10                  15      
Ala Thr Ser Phe Gly Val Leu Ala
            20                  


<210> 34
<211> 72
<212> DNA
<213> Pseudomonas fluorescens

<220> 
<221> CDS            
<222> (1)...(72)

<400> 34
atg aaa ctg aaa cgt ttg atg gcg gca atg act ttt gtc gct gct ggc   48
Met Lys Leu Lys Arg Leu Met Ala Ala Met Thr Phe Val Ala Ala Gly
 1               5                   10                  15
 
gtt gcg acc gcc aac gcg gtg gcc                                   72
Val Ala Thr Ala Asn Ala Val Ala
             20
 

<210> 35
<211> 24
<212> PRT
<213> Pseudomonas fluorescens

<400> 35
Met Lys Leu Lys Arg Leu Met Ala Ala Met Thr Phe Val Ala Ala Gly
 1               5                  10                  15      
Val Ala Thr Ala Asn Ala Val Ala
            20                  


<210> 36
<211> 60
<212> DNA
<213> Pseudomonas fluorescens

<220> 
<221> CDS            
<222> (1)...(60)

<400> 36
atg ttt gcc aaa ctc gtt gct gtt tcc ctg ctg act ctg gcg agc ggc   48
Met Phe Ala Lys Leu Val Ala Val Ser Leu Leu Thr Leu Ala Ser Gly
 1               5                   10                  15
 
cag ttg ctt gct                                                   60
Gln Leu Leu Ala
             20
 

<210> 37
<211> 20
<212> PRT
<213> Pseudomonas fluorescens

<400> 37
Met Phe Ala Lys Leu Val Ala Val Ser Leu Leu Thr Leu Ala Ser Gly
 1               5                  10                  15      
Gln Leu Leu Ala
            20  


<210> 38
<211> 51
<212> DNA
<213> Pseudomonas fluorescens

<220> 
<221> CDS            
<222> (1)...(51)

<400> 38
atg atc aaa cgc aat ctg ctg gtt atg ggc ctt gcc gtg ctg ttg agc   48
Met Ile Lys Arg Asn Leu Leu Val Met Gly Leu Ala Val Leu Leu Ser
 1               5                   10                  15
 
gct                                                               51
Ala

 

<210> 39
<211> 17
<212> PRT
<213> Pseudomonas fluorescens

<400> 39
Met Ile Lys Arg Asn Leu Leu Val Met Gly Leu Ala Val Leu Leu Ser
 1               5                  10                  15      
Ala
    


<210> 40
<211> 69
<212> DNA
<213> Pseudomonas fluorescens

<220> 
<221> CDS            
<222> (1)...(69)

<400> 40
atg cag aac tat aaa aaa ttc ctt ctg gcc gcg gcc gtc tcg atg gcg   48
Met Gln Asn Tyr Lys Lys Phe Leu Leu Ala Ala Ala Val Ser Met Ala
 1               5                   10                  15
 
ttc agc gcc acg gcc atg gca                                       69
Phe Ser Ala Thr Ala Met Ala
             20
 

<210> 41
<211> 23
<212> PRT
<213> Pseudomonas fluorescens

<400> 41
Met Gln Asn Tyr Lys Lys Phe Leu Leu Ala Ala Ala Val Ser Met Ala
 1               5                  10                  15      
Phe Ser Ala Thr Ala Met Ala
            20              


<210> 42
<211> 93
<212> DNA
<213> Pseudomonas fluorescens

<220> 
<221> CDS            
<222> (1)...(93)

<400> 42
atg atc cgt gac aac cga ctc aag aca tcc ctt ctg cgc ggc ctg acc   48
Met Ile Arg Asp Asn Arg Leu Lys Thr Ser Leu Leu Arg Gly Leu Thr
 1               5                   10                  15
 
ctc acc cta ctc agc ctg acc ctg ctc tcg ccc gcg gcc cat tct       93
Leu Thr Leu Leu Ser Leu Thr Leu Leu Ser Pro Ala Ala His Ser
             20                  25                  30
 


<210> 43
<211> 31
<212> PRT
<213> Pseudomonas fluorescens

<400> 43
Met Ile Arg Asp Asn Arg Leu Lys Thr Ser Leu Leu Arg Gly Leu Thr
 1               5                  10                  15      
Leu Thr Leu Leu Ser Leu Thr Leu Leu Ser Pro Ala Ala His Ser
            20                  25                  30      


<210> 44
<211> 5
<212> PRT
<213> Artificial Sequence

<220> 
<223> N-terminal amino acid sequence of processed azurin
      and ibp
      
      
      

<400> 44
Ala Gln Val Gln Leu
 1               5  


<210> 45
<211> 1317
<212> DNA
<213> Bacillus coagulans

<220> 
<221> CDS            
<222> (1)...(1317)

<400> 45
atg agc aca cga atc ccc cgc cga caa tgg ctg aaa ggc gcc tcg ggc   48
Met Ser Thr Arg Ile Pro Arg Arg Gln Trp Leu Lys Gly Ala Ser Gly
 1               5                   10                  15
 
ctg ctg gcc gcc gcg agc ctg ggc cgg ttg gcc aac cgc gag gcg cgc   96
Leu Leu Ala Ala Ala Ser Leu Gly Arg Leu Ala Asn Arg Glu Ala Arg
             20                  25                  30
 
gcc gcc gaa gcg agc gcc gcc gcg ccg ctc gac act ggc tcg ctg ggc   144
Ala Ala Glu Ala Ser Ala Ala Ala Pro Leu Asp Thr Gly Ser Leu Gly
         35                  40                  45
 
gcc tcg ccg cgc gcg acg ctc gac gcc tgc ctg caa aaa gcc gtc gac   192
Ala Ser Pro Arg Ala Thr Leu Asp Ala Cys Leu Gln Lys Ala Val Asp
     50                  55                  60
 
gac ggc acg ctc aag agc gtg gtg gcg atg gcc gcc acc gag cgc ggg   240
Asp Gly Thr Leu Lys Ser Val Val Ala Met Ala Ala Thr Glu Arg Gly
 65                  70                  75                  80
 
ctc gcc tac cag ggc gcg cgc ggc ccg gcc aac gcg gcc ggc gag ccg   288
Leu Ala Tyr Gln Gly Ala Arg Gly Pro Ala Asn Ala Ala Gly Glu Pro
                 85                  90                  95
 
atc ggc ccc gat acg gtg ttc tgg atg ctg tcg atg acc aag gcg atc   336
Ile Gly Pro Asp Thr Val Phe Trp Met Leu Ser Met Thr Lys Ala Ile
            100                 105                 110
 
acc gcc acc gcc tgc atg cag ctg atc gag cag ggc cgg ctc ggg ctc   384
Thr Ala Thr Ala Cys Met Gln Leu Ile Glu Gln Gly Arg Leu Gly Leu
        115                 120                 125
 
gac cag ccc gcc gcc gag atc ctg ccg caa ctg aag gcg ccg cag gtg   432
Asp Gln Pro Ala Ala Glu Ile Leu Pro Gln Leu Lys Ala Pro Gln Val
    130                 135                 140
 
ctg gag ggc ttc gac gcc gcc ggc cag ccc agg ctg cgc ccg gcg cgc   480
Leu Glu Gly Phe Asp Ala Ala Gly Gln Pro Arg Leu Arg Pro Ala Arg
145                 150                 155                 160
 
cgc gcg atc acg gtg cgc cac ctg ctc acg cat acc tcg ggc tat acc   528
Arg Ala Ile Thr Val Arg His Leu Leu Thr His Thr Ser Gly Tyr Thr
                165                 170                 175
 
tac agc atc tgg agc gag gcg ctg ggc cgc tac gaa cag gtc acg ggc   576
Tyr Ser Ile Trp Ser Glu Ala Leu Gly Arg Tyr Glu Gln Val Thr Gly
            180                 185                 190
 
atg ccc gac atc ggc tac tcg ctg aac ggc gcc ttc gcg gcc ccg ctc   624
Met Pro Asp Ile Gly Tyr Ser Leu Asn Gly Ala Phe Ala Ala Pro Leu
        195                 200                 205
 
gaa ttc gag ccc ggc gag cgc tgg caa tac ggc atc ggc atg gat tgg   672
Glu Phe Glu Pro Gly Glu Arg Trp Gln Tyr Gly Ile Gly Met Asp Trp
    210                 215                 220
 
gtg ggc aag ctg gtg gag gcg gtg acc gac cag tcg ctg gaa gtg gcg   720
Val Gly Lys Leu Val Glu Ala Val Thr Asp Gln Ser Leu Glu Val Ala
225                 230                 235                 240
 
ttc cgc gag cgg atc ttc gcg ccg ctc ggc atg cac gat acg ggc ttc   768
Phe Arg Glu Arg Ile Phe Ala Pro Leu Gly Met His Asp Thr Gly Phe
                245                 250                 255
 
ctg atc ggc agc gcg caa aag cgc cgc gtc gcc acg ctg cat cgg cgc   816
Leu Ile Gly Ser Ala Gln Lys Arg Arg Val Ala Thr Leu His Arg Arg
            260                 265                 270
 
cag gcc gat ggc tcg ctg acg ccg gaa ccc ttc gag acc aac cag cgg   864
Gln Ala Asp Gly Ser Leu Thr Pro Glu Pro Phe Glu Thr Asn Gln Arg
        275                 280                 285
 
ccc gag ttc ttc atg ggc ggc ggc ggg ctg ttc agc acc ccg cgc gac   912
Pro Glu Phe Phe Met Gly Gly Gly Gly Leu Phe Ser Thr Pro Arg Asp
    290                 295                 300
 
tac ctc gcc ttc ctg cag atg ctg ctg aac ggc ggc gcc tgg cgc ggc   960
Tyr Leu Ala Phe Leu Gln Met Leu Leu Asn Gly Gly Ala Trp Arg Gly
305                 310                 315                 320
 
gag cgg ctg ctg cgg ccc gac acc gtg gcg agc atg ttc cgc aac cag   1008
Glu Arg Leu Leu Arg Pro Asp Thr Val Ala Ser Met Phe Arg Asn Gln
                325                 330                 335
 
atc ggc gat ctt cag gtt cgc gaa atg aag acc gcc cag ccg gcc tgg   1056
Ile Gly Asp Leu Gln Val Arg Glu Met Lys Thr Ala Gln Pro Ala Trp
            340                 345                 350
 
tcg aac agc ttc gac caa ttc ccc ggc gcg acg cac aag tgg ggg ctg   1104
Ser Asn Ser Phe Asp Gln Phe Pro Gly Ala Thr His Lys Trp Gly Leu
        355                 360                 365
 
tcc ttc gat ctc aac agc gag ccg ggg ccg cac ggg cgc ggc gcc ggc   1152
Ser Phe Asp Leu Asn Ser Glu Pro Gly Pro His Gly Arg Gly Ala Gly
    370                 375                 380
 
tcg ggt agc tgg gcc ggc ctg ctg aac acc tac ttc tgg atc gat ccc   1200
Ser Gly Ser Trp Ala Gly Leu Leu Asn Thr Tyr Phe Trp Ile Asp Pro
385                 390                 395                 400
 
gcc aag cgc gtg acg ggg gcg ctg ttc acg cag atg ctg ccg ttc tac   1248
Ala Lys Arg Val Thr Gly Ala Leu Phe Thr Gln Met Leu Pro Phe Tyr
                405                 410                 415
 
gac gcg cgc gtg gtc gat ctc tac ggg cgc ttc gag cgc ggg ctc tac   1296
Asp Ala Arg Val Val Asp Leu Tyr Gly Arg Phe Glu Arg Gly Leu Tyr
            420                 425                 430
 
gac ggg ctg ggc cgc gcc tga                                       1317
Asp Gly Leu Gly Arg Ala  *
        435
 

<210> 46
<211> 438
<212> PRT
<213> Bacillus coagulans

<400> 46
Met Ser Thr Arg Ile Pro Arg Arg Gln Trp Leu Lys Gly Ala Ser Gly
 1               5                  10                  15      
Leu Leu Ala Ala Ala Ser Leu Gly Arg Leu Ala Asn Arg Glu Ala Arg
            20                  25                  30          
Ala Ala Glu Ala Ser Ala Ala Ala Pro Leu Asp Thr Gly Ser Leu Gly
        35                  40                  45              
Ala Ser Pro Arg Ala Thr Leu Asp Ala Cys Leu Gln Lys Ala Val Asp
    50                  55                  60                  
Asp Gly Thr Leu Lys Ser Val Val Ala Met Ala Ala Thr Glu Arg Gly
65                  70                  75                  80  
Leu Ala Tyr Gln Gly Ala Arg Gly Pro Ala Asn Ala Ala Gly Glu Pro
                85                  90                  95      
Ile Gly Pro Asp Thr Val Phe Trp Met Leu Ser Met Thr Lys Ala Ile
            100                 105                 110         
Thr Ala Thr Ala Cys Met Gln Leu Ile Glu Gln Gly Arg Leu Gly Leu
        115                 120                 125             
Asp Gln Pro Ala Ala Glu Ile Leu Pro Gln Leu Lys Ala Pro Gln Val
    130                 135                 140                 
Leu Glu Gly Phe Asp Ala Ala Gly Gln Pro Arg Leu Arg Pro Ala Arg
145                 150                 155                 160 
Arg Ala Ile Thr Val Arg His Leu Leu Thr His Thr Ser Gly Tyr Thr
                165                 170                 175     
Tyr Ser Ile Trp Ser Glu Ala Leu Gly Arg Tyr Glu Gln Val Thr Gly
            180                 185                 190         
Met Pro Asp Ile Gly Tyr Ser Leu Asn Gly Ala Phe Ala Ala Pro Leu
        195                 200                 205             
Glu Phe Glu Pro Gly Glu Arg Trp Gln Tyr Gly Ile Gly Met Asp Trp
    210                 215                 220                 
Val Gly Lys Leu Val Glu Ala Val Thr Asp Gln Ser Leu Glu Val Ala
225                 230                 235                 240 
Phe Arg Glu Arg Ile Phe Ala Pro Leu Gly Met His Asp Thr Gly Phe
                245                 250                 255     
Leu Ile Gly Ser Ala Gln Lys Arg Arg Val Ala Thr Leu His Arg Arg
            260                 265                 270         
Gln Ala Asp Gly Ser Leu Thr Pro Glu Pro Phe Glu Thr Asn Gln Arg
        275                 280                 285             
Pro Glu Phe Phe Met Gly Gly Gly Gly Leu Phe Ser Thr Pro Arg Asp
    290                 295                 300                 
Tyr Leu Ala Phe Leu Gln Met Leu Leu Asn Gly Gly Ala Trp Arg Gly
305                 310                 315                 320 
Glu Arg Leu Leu Arg Pro Asp Thr Val Ala Ser Met Phe Arg Asn Gln
                325                 330                 335     
Ile Gly Asp Leu Gln Val Arg Glu Met Lys Thr Ala Gln Pro Ala Trp
            340                 345                 350         
Ser Asn Ser Phe Asp Gln Phe Pro Gly Ala Thr His Lys Trp Gly Leu
        355                 360                 365             
Ser Phe Asp Leu Asn Ser Glu Pro Gly Pro His Gly Arg Gly Ala Gly
    370                 375                 380                 
Ser Gly Ser Trp Ala Gly Leu Leu Asn Thr Tyr Phe Trp Ile Asp Pro
385                 390                 395                 400 
Ala Lys Arg Val Thr Gly Ala Leu Phe Thr Gln Met Leu Pro Phe Tyr
                405                 410                 415     
Asp Ala Arg Val Val Asp Leu Tyr Gly Arg Phe Glu Arg Gly Leu Tyr
            420                 425                 430         
Asp Gly Leu Gly Arg Ala
        435             


<210> 47
<211> 324
<212> DNA
<213> Escherichia coli

<220> 
<221> CDS            
<222> (1)...(324)

<400> 47
agc gat aaa att att cac ctg act gac gac agt ttt gac acg gat gta   48
Ser Asp Lys Ile Ile His Leu Thr Asp Asp Ser Phe Asp Thr Asp Val
 1               5                   10                  15
 
ctc aaa gcg gac ggg gcg atc ctc gtc gat ttc tgg gca gag tgg tgc   96
Leu Lys Ala Asp Gly Ala Ile Leu Val Asp Phe Trp Ala Glu Trp Cys
             20                  25                  30
 
ggt ccg tgc aaa atg atc gcc ccg att ctg gat gaa atc gct gac gaa   144
Gly Pro Cys Lys Met Ile Ala Pro Ile Leu Asp Glu Ile Ala Asp Glu
         35                  40                  45
 
tat cag ggc aaa ctg acc gtt gca aaa ctg aac atc gat caa aac cct   192
Tyr Gln Gly Lys Leu Thr Val Ala Lys Leu Asn Ile Asp Gln Asn Pro
     50                  55                  60
 
ggc act gcg ccg aaa tat ggc atc cgt ggt atc ccg act ctg ctg ctg   240
Gly Thr Ala Pro Lys Tyr Gly Ile Arg Gly Ile Pro Thr Leu Leu Leu
 65                  70                  75                  80
 
ttc aaa aac ggt gaa gtg gcg gca acc aaa gtg ggt gca ctg tct aaa   288
Phe Lys Asn Gly Glu Val Ala Ala Thr Lys Val Gly Ala Leu Ser Lys
                 85                  90                  95
 
ggt cag ttg aaa gag ttc ctc gac gct aac ctg gcg                   324
Gly Gln Leu Lys Glu Phe Leu Asp Ala Asn Leu Ala
            100                 105
 

<210> 48
<211> 108
<212> PRT
<213> Escherichia coli

<400> 48
Ser Asp Lys Ile Ile His Leu Thr Asp Asp Ser Phe Asp Thr Asp Val
 1               5                  10                  15      
Leu Lys Ala Asp Gly Ala Ile Leu Val Asp Phe Trp Ala Glu Trp Cys
            20                  25                  30          
Gly Pro Cys Lys Met Ile Ala Pro Ile Leu Asp Glu Ile Ala Asp Glu
        35                  40                  45              
Tyr Gln Gly Lys Leu Thr Val Ala Lys Leu Asn Ile Asp Gln Asn Pro
    50                  55                  60                  
Gly Thr Ala Pro Lys Tyr Gly Ile Arg Gly Ile Pro Thr Leu Leu Leu
65                  70                  75                  80  
Phe Lys Asn Gly Glu Val Ala Ala Thr Lys Val Gly Ala Leu Ser Lys
                85                  90                  95      
Gly Gln Leu Lys Glu Phe Leu Asp Ala Asn Leu Ala
            100                 105             


<210> 49
<211> 63
<212> DNA
<213> Pseudomonas fluorescens

<220> 
<221> CDS            
<222> (1)...(63)

<400> 49
atg aga aac ctt ctt cga gga atg ctt gtc gtt att tgc tgt atg gca   48
Met Arg Asn Leu Leu Arg Gly Met Leu Val Val Ile Cys Cys Met Ala
 1               5                   10                  15
 
ggg ata gcg gcg gcg                                               63
Gly Ile Ala Ala Ala
             20
 

<210> 50
<211> 21
<212> PRT
<213> Pseudomonas fluorescens

<400> 50
Met Arg Asn Leu Leu Arg Gly Met Leu Val Val Ile Cys Cys Met Ala
 1               5                  10                  15      
Gly Ile Ala Ala Ala
            20      

