                         SEQUENCE LISTING

<110>  Scottish Crop Research Institute
 
<120>  Inhibition of Gene Expression

<130>  P49169.WO.01

<150>  GB 0701722.1
<151>  2007-01-30

<160>  26    

<170>  PatentIn version 3.2

<210>  1
<211>  10
<212>  DNA
<213>  Artificial

<220>
<223>  5'ss sequence

<400>  1
maggtragta                                                            10


<210>  2
<211>  10
<212>  DNA
<213>  Artificial

<220>
<223>  Embodiment of 5'ss sequence

<400>  2
caggtaagta                                                            10


<210>  3
<211>  308
<212>  DNA
<213>  Artificial

<220>
<223>  Nucleotidic composition of the 3' UTR of GFP-2x5' ss

<400>  3
taagagctcg agcaggtaag taggcgcgcc caggtaagta gagctcgaat ttccccgatc     60

gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga    120

ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga    180

cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga    240

tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt    300

tactagat                                                             308


<210>  4
<211>  855
<212>  DNA
<213>  Artificial

<220>
<223>  Nucleotidic composition of the 3' UTR of GFP-MCS-2x5' ss-OCS

<400>  4
taaccctagg tacgtaggcg cgccattata aatctagaca ggtaagtacg gatccgcagg     60

taagtagacg tcctctagct taattaagac ccgggactag tccctagagt cctgctttaa    120

tgagatatgc gagacgccta tgatcctgct ttaatgagat atgcgagacg cctatgatcg    180

catgatattt gctttcaatt ctgttgtgca cgttgtaaaa aacctgagca tgtgtagctc    240

agatccttac cgccggtttc ggttcattct aatgaatata tcacccgtta ctatcgtatt    300

tttatgaata atattctccg ttcaatttac tgattgtacc ctactactta tatgtacaat    360

attaaaatga aaacaatata ttgtgctgaa taggtttata gcgacatcta tgatagagcg    420

ccacaataac aaacaattgc gttttattat tacaaatcca attttaaaaa aagcggcaga    480

accggtcaaa cctaaaagac tgattacata aatcttattc aaatttcaaa agtgccccag    540

gggctagtat ctacgacaca ccgagcggcg aactaataac gctcactgaa gggaactccg    600

gttccccgcc ggcgcgcatg ggtgagattc cttgaagttg agtattggcc gtccgctcta    660

ccgaaagtta cgggcaccat tcaacccggt ccagcacggc ggccgggtaa ccgacttgct    720

gccccgagaa ttatgcagca tttttttggt gtatgtgggc cccaaatgaa gtgcaggtca    780

aaccttgaca gtgacgacaa atcgttgggc gggtccaggg cgaattttgc gacaacatgt    840

cgaggctgag cagga                                                     855


<210>  5
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Forward primer used for semi-quantitative RT-PCR of GFP 
       expression

<400>  5
gggcacaaat tttctgtcag                                                 20


<210>  6
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  Reverse primer used for semi-quantitative RT-PCR of GFP 
       expression

<400>  6
gttgtgggag ttgtagttgt attc                                            24


<210>  7
<211>  31
<212>  DNA
<213>  Artificial

<220>
<223>  REV1 primer

<400>  7
aaataacgtc atgcattaca tgttaattat t                                    31


<210>  8
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  REV2 primer

<400>  8
ttctatcgcg tattaaatgt ataattg                                         27


<210>  9
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  forward primer

<400>  9
tccacacaat ctgccctttc                                                 20


<210>  10
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  reverse primer

<400>  10
gcgagctccg cggccttttt ttttttt                                         27


<210>  11
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  Primer for amplification of CaMV 35s::mgfp5-ER::3'-NOS derived 
       from pBINmgfp5-ER plasmid

<400>  11
cccaagcttt ttcagaaaga atgctaaccc                                      30


<210>  12
<211>  32
<212>  DNA
<213>  Artificial

<220>
<223>  Primer for amplification of CaMV 35s::mgfp5-ER::3'-NOS derived 
       from pBINmgfp5-ER plasmid

<400>  12
cccaagcttg atctagtaac atagatgaca cc                                   32


<210>  13
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  self annealing primer

<400>  13
cgagmaggtr agtaggcgcg ccgagct                                         27


<210>  14
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  self annealing primer

<400>  14
cggcgcgcct acttacctgc tcgagct                                         27


<210>  15
<211>  37
<212>  DNA
<213>  Artificial

<220>
<223>  self annealing primer

<400>  15
cgagmaggtr agtaggcgcg ccmaggtrag tagagct                              37


<210>  16
<211>  37
<212>  DNA
<213>  Artificial

<220>
<223>  self annealing primer

<400>  16
ctacttacct gggcgcgcct acttacctgc tcgagct                              37


<210>  17
<211>  45
<212>  DNA
<213>  Artificial

<220>
<223>  Primer used for amplification of GFP ORF from pBINmgfp5-ER 
       plasmid

<400>  17
gtgtgtctcg agccatggcc aagactaatc tttttctctt tctca                     45


<210>  18
<211>  57
<212>  DNA
<213>  Artificial

<220>
<223>  Primer used for amplification of GFP ORF from pBINmgfp5-ER 
       plasmid

<400>  18
gtgtgtggcg cgcctacgta cctagggtta accaagctca tcatgtttgt atagttc        57


<210>  19
<211>  62
<212>  DNA
<213>  Artificial

<220>
<223>  Annealed primer used for ligating ChSA intron sequences

<400>  19
cgcgccatta taaatctaga caggtaagta cggatccgca ggtaagtaga cgtcctctag     60

cc                                                                    62


<210>  20
<211>  62
<212>  DNA
<213>  Artificial

<220>
<223>  Annealed primer for ligating ChSA intron sequences

<400>  20
ccggggctag aggacgtcta cttacctgcg gatccgtact tacctgtcta gatttataat     60

gg                                                                    62


<210>  21
<211>  33
<212>  DNA
<213>  Artificial

<220>
<223>  Forward primer for amplification of 314 bp PDS cDNA fragment

<400>  21
atgggatcca tgaaggaact agcgaagctt ttc                                  33


<210>  22
<211>  35
<212>  DNA
<213>  Artificial

<220>
<223>  Reverse primer for amplification of 314 bp PDS cDNA fragment

<400>  22
tacgagctct tagttcacta tgctaactac gcttg                                35


<210>  23
<211>  51
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  23
cgcgccgatg cagatattcg taattatgcg ggcaacgtct ggtatcagcg g              51


<210>  24
<211>  51
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  24
cgcgccgctg ataccagacg ttgcccgcat aattacgaat atctgcatcg g              51


<210>  25
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  Primer 1 for GUS amplification (1000 bp PCR product)

<400>  25
aaggcgcgcc gatgcagata ttc                                             23


<210>  26
<211>  26
<212>  DNA
<213>  Artificial

<220>
<223>  Primer 2 for GUS amplification (1000 bp PCR product)

<400>  26
aaggcgcgcc gcgcttgctg agtttc                                          26


