                               SEQUENCE LISTING

<110> GENERAL ELECTRIC COMPANY
 
<120> ISOTHERMAL DNA AMPLIFICATION

<130> PG07006

<140>
<141>

<150> 11/621,703
<151> 2007-01-10

<160> 55    

<170> PatentIn version 3.3

<210> 1
<211> 231
<212> PRT
<213> Escherichia coli

<400> 1
Met Asp Leu Ala Ser Leu Arg Ala Gln Gln Ile Glu Leu Ala Ser Ser 
1               5                   10                  15      


Val Ile Arg Glu Asp Arg Leu Asp Lys Asp Pro Pro Asp Leu Ile Ala 
            20                  25                  30          


Gly Ala Asp Val Gly Phe Glu Gln Gly Gly Glu Val Thr Arg Ala Ala 
        35                  40                  45              


Met Val Leu Leu Lys Tyr Pro Ser Leu Glu Leu Val Glu Tyr Lys Val 
    50                  55                  60                  


Ala Arg Ile Ala Thr Thr Met Pro Tyr Ile Pro Gly Phe Leu Ser Phe 
65                  70                  75                  80  


Arg Glu Tyr Pro Ala Leu Leu Ala Ala Trp Glu Met Leu Ser Gln Lys 
                85                  90                  95      


Pro Asp Leu Val Phe Val Asp Gly His Gly Ile Ser His Pro Arg Arg 
            100                 105                 110         


Leu Gly Val Ala Ser His Phe Gly Leu Leu Val Asp Val Pro Thr Ile 
        115                 120                 125             


Gly Val Ala Lys Lys Arg Leu Cys Gly Lys Phe Glu Pro Leu Ser Ser 
    130                 135                 140                 


Glu Pro Gly Ala Leu Ala Pro Leu Met Asp Lys Gly Glu Gln Leu Ala 
145                 150                 155                 160 


Trp Val Trp Arg Ser Lys Ala Arg Cys Asn Pro Leu Phe Ile Ala Thr 
                165                 170                 175     


Gly His Arg Val Ser Val Asp Ser Ala Leu Ala Trp Val Gln Arg Cys 
            180                 185                 190         


Met Lys Gly Tyr Arg Leu Pro Glu Pro Thr Arg Trp Ala Asp Ala Val 
        195                 200                 205             


Ala Ser Glu Arg Pro Ala Phe Val Arg Tyr Thr Ala Asn Gln Pro Leu 
    210                 215                 220                 


Glu His His His His His His 
225                 230     



<210> 2
<211> 231
<212> PRT
<213> Escherichia coli

<400> 2
Met Asp Leu Ala Ser Leu Arg Ala Gln Gln Ile Glu Leu Ala Ser Ser 
1               5                   10                  15      


Val Ile Arg Glu Asp Arg Leu Asp Lys Asp Pro Pro Asp Leu Ile Ala 
            20                  25                  30          


Gly Ala Asp Val Gly Phe Glu Gln Gly Gly Glu Val Thr Arg Ala Ala 
        35                  40                  45              


Met Val Leu Leu Lys Tyr Pro Ser Leu Glu Leu Val Glu Tyr Lys Val 
    50                  55                  60                  


Ala Arg Ile Ala Thr Thr Met Pro Ala Ile Pro Gly Phe Leu Ser Phe 
65                  70                  75                  80  


Arg Glu Tyr Pro Ala Leu Leu Ala Ala Trp Glu Met Leu Ser Gln Lys 
                85                  90                  95      


Pro Asp Leu Val Phe Val Asp Gly His Gly Ile Ser His Pro Arg Arg 
            100                 105                 110         


Leu Gly Val Ala Ser His Phe Gly Leu Leu Val Asp Val Pro Thr Ile 
        115                 120                 125             


Gly Val Ala Lys Lys Arg Leu Cys Gly Lys Phe Glu Pro Leu Ser Ser 
    130                 135                 140                 


Glu Pro Gly Ala Leu Ala Pro Leu Met Asp Lys Gly Glu Gln Leu Ala 
145                 150                 155                 160 


Trp Val Trp Arg Ser Lys Ala Arg Cys Asn Pro Leu Phe Ile Ala Thr 
                165                 170                 175     


Gly His Arg Val Ser Val Asp Ser Ala Leu Ala Trp Val Gln Arg Cys 
            180                 185                 190         


Met Lys Gly Tyr Arg Leu Pro Glu Pro Thr Arg Trp Ala Asp Ala Val 
        195                 200                 205             


Ala Ser Glu Arg Pro Ala Phe Val Arg Tyr Thr Ala Asn Gln Pro Leu 
    210                 215                 220                 


Glu His His His His His His 
225                 230     


<210> 3
<211> 221
<212> PRT
<213> Archaeoglobus fulgidus

<400> 3
Met Leu Gln Met Asn Leu Glu Glu Leu Arg Arg Ile Gln Glu Glu Met 
1               5                   10                  15      


Ser Arg Ser Val Val Leu Glu Asp Leu Ile Pro Leu Glu Glu Leu Glu 
            20                  25                  30          


Tyr Val Val Gly Val Asp Gln Ala Phe Ile Ser Asp Glu Val Val Ser 
        35                  40                  45              


Cys Ala Val Lys Leu Thr Phe Pro Glu Leu Glu Val Val Asp Lys Ala 
    50                  55                  60                  


Val Arg Val Glu Lys Val Thr Phe Pro Ala Ile Pro Thr Phe Leu Met 
65                  70                  75                  80  


Phe Arg Glu Gly Glu Pro Ala Val Asn Ala Val Lys Gly Leu Val Asp 
                85                  90                  95      


Asp Arg Ala Ala Ile Met Val Asp Gly Ser Gly Ile Ala His Pro Arg 
            100                 105                 110         


Arg Cys Gly Leu Ala Thr Tyr Ile Ala Leu Lys Leu Arg Lys Pro Thr 
        115                 120                 125             


Val Gly Ile Thr Lys Lys Arg Leu Phe Gly Glu Met Val Glu Val Glu 
    130                 135                 140                 


Asp Gly Leu Trp Arg Leu Leu Asp Gly Ser Glu Thr Ile Gly Tyr Ala 
145                 150                 155                 160 


Leu Lys Ser Cys Arg Arg Cys Lys Pro Ile Phe Ile Ser Pro Gly Ser 
                165                 170                 175     


Tyr Ile Ser Pro Asp Ser Ala Leu Glu Leu Thr Arg Lys Cys Leu Lys 
            180                 185                 190         



Gly Tyr Lys Leu Pro Glu Pro Ile Arg Ile Ala Asp Lys Leu Thr Lys 
        195                 200                 205             


Glu Val Lys Arg Glu Leu Thr Pro Thr Ser Lys Leu Lys 
    210                 215                 220     


<210> 4
<211> 77
<212> DNA
<213> Bacillus cereus

<400> 4
caattgtaat ttctgtacgt ctcttatcat tgaagcgctc ttttacttct gttaattctt     60

cacgaataat ctcaaga                                                    77


<210> 5
<211> 16
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic 
      oligonucleotide


<220>
<221> modified_base
<222> (15)..(15)
<223> inosine

<400> 5
tcttgagatt attcnt                                                     16


<210> 6
<211> 16
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic 
      oligonucleotide


<220>
<221> modified_base
<222> (15)..(15)
<223> inosine 

<400> 6
caattgtaat ttctnt                                                     16


<210> 7
<211> 51
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic 
      oligonucleotide

<220>
<221> modified_base
<222> (51)..(51)
<223> dideoxycytidine 


<400> 7
aaattaatac gactcactat agggtgaaga attaacagaa gtaaaagagc c              51


<210> 8
<211> 51
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic 
      oligonucleotide

<400> 8
aaattaatac gactcactat agggttgaag aattaacaga agtaaaagag a              51


<210> 9
<211> 41
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic 
      oligonucleotide

<400> 9
aaattaatac gactcactat agggtgaaga attaacagaa g                         41


<210> 10
<211> 51
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic 
      oligonucleotide

<220>
<221> modified_base
<222> (51)..(51)
<223> dideoxycytidine 

<400> 10
aaattaatac gactcactat agggttgaag aattaacaga agtaaaagag c              51


<210> 11
<211> 16
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic 
      oligonucleotide


<220>
<221> modified_base
<222> (15)..(15)
<223> inosine

<400> 11
tcgctgaatt aaaanc                                                     16


<210> 12
<211> 18
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic 
      oligonucleotide


<220>
<221> modified_base
<222> (17)..(17)
<223> inosine 

<400> 12
atcaagattt aatgaant                                                   18


<210> 13
<211> 16
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic 
      oligonucleotide


<220>
<221> modified_base
<222> (15)..(15)
<223> inosine 

<400> 13
tgttctggaa tcaant                                                     16


<210> 14
<211> 16
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic 
      oligonucleotide


<220>
<221> modified_base
<222> (15)..(15)
<223> inosine 

<400> 14
aacgtaatgg cgatnt                                                     16


<210> 15
<211> 16
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic 
      oligonucleotide


<220>
<221> modified_base
<222> (15)..(15)
<223> inosine 

<400> 15
ttagatatgc gtctnc                                                     16


<210> 16
<211> 16
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic 
      oligonucleotide


<220>
<221> modified_base
<222> (15)..(15)
<223> inosine 

<400> 16
gcttaacagg attana                                                     16


<210> 17
<211> 18
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic 
      oligonucleotide


<220>
<221> modified_base
<222> (17)..(17)
<223> inosine 

<400> 17
cgaaaaaatt gaacaana                                                   18


<210> 18
<211> 16
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic 
      oligonucleotide


<220>
<221> modified_base
<222> (15)..(15)
<223> inosine 

<400> 18
cagatgaaga aaagnt                                                     16


<210> 19
<211> 16
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic 
      oligonucleotide


<220>
<221> modified_base
<222> (15)..(15)
<223> inosine 

<400> 19
cttcatcttc aatana                                                     16


<210> 20
<211> 18
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic 
      oligonucleotide


<220>
<221> modified_base
<222> (17)..(17)
<223> inosine 

<400> 20
aatataacca ttatgant                                                   18


<210> 21
<211> 15
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic 
      oligonucleotide


<220>
<221> modified_base
<222> (14)..(14)
<223> inosine 

<400> 21
tacgtagaag ctgnc                                                      15


<210> 22
<211> 15
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic 
      oligonucleotide


<220>
<221> modified_base
<222> (14)..(14)

<223> inosine 

<400> 22
accacggttc tgtnt                                                      15


<210> 23
<211> 24
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic 
      oligonucleotide

<400> 23
ccctatagtg agtcgtatta attt                                            24


<210> 24
<211> 27
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic 
      oligonucleotide


<220>
<221> modified_base
<222> (9)..(9)
<223> inosine 

<400> 24
ggtcgactna ggaggatccc cgggtac                                         27


<210> 25
<211> 27
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic 
      oligonucleotide

<400> 25
ccggggatcc tcctcagtcg acctgca                                         27


<210> 26
<211> 23
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic 
      oligonucleotide

<400> 26
gagatataca tatggatctc gcg                                             23


<210> 27
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic 
      oligonucleotide

<400> 27
gaatcgccgg catggtggtg gcgatgcg                                        28


<210> 28
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic 
      oligonucleotide

<400> 28
accatgccgg cgattccagg ttttctttcc ttc                                  33


<210> 29
<211> 25
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic 
      oligonucleotide

<400> 29
tggtgctcga ggggctgatt tgatg                                           25


<210> 30
<211> 27
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic 
      oligonucleotide

<400> 30
gatattcatc aatcggagta cgttttc                                         27


<210> 31
<211> 27
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic 
      oligonucleotide

<400> 31
acaatcaaca acaagcacga attcgag                                         27


<210> 32
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic 
      oligonucleotide


<220>
<221> modified_base
<222> (19)..(19)
<223> inosine 

<400> 32
agttcttctt tcgtccccnt                                                 20


<210> 33
<211> 17
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic 
      oligonucleotide


<220>
<221> modified_base
<222> (15)..(16)
<223> inosine 

<400> 33
caggctgaca tcacnnt                                                    17


<210> 34
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic 
      oligonucleotide


<220>
<221> modified_base
<222> (18)..(18)
<223> inosine 

<400> 34
tcagttgttc acccagcna                                                  19


<210> 35
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic 
      oligonucleotide


<220>
<221> modified_base
<222> (18)..(18)
<223> inosine 

<400> 35
gcggagacgg gcaatcant                                                  19


<210> 36
<211> 17
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic 
      oligonucleotide


<220>
<221> modified_base
<222> (15)..(16)
<223> inosine 

<400> 36
tcatctttcg tcatnna                                                    17


<210> 37
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic 
      oligonucleotide


<220>
<221> modified_base
<222> (17)..(18)
<223> inosine 

<400> 37
tccacagaga aacaatnnc                                                  19


<210> 38
<211> 17

<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic 
      oligonucleotide


<220>
<221> modified_base
<222> (15)..(16)
<223> inosine 

<400> 38
accaccggcg atccnnc                                                    17


<210> 39
<211> 17
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic 
      oligonucleotide


<220>
<221> modified_base
<222> (16)..(16)
<223> inosine 

<400> 39
gcgtgagttc accatna                                                    17


<210> 40
<211> 18
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic 
      oligonucleotide


<220>
<221> modified_base
<222> (17)..(17)
<223> inosine 

<400> 40
ttcagtcagc accgctna                                                   18


<210> 41
<211> 16
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic 
      oligonucleotide



<220>
<221> modified_base
<222> (15)..(15)
<223> inosine 

<400> 41
tgatgctgct ggctna                                                     16


<210> 42
<211> 17
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic 
      oligonucleotide


<220>
<221> modified_base
<222> (16)..(16)
<223> inosine 

<400> 42
ccctgatgag ttcgtnt                                                    17


<210> 43
<211> 15
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic 
      oligonucleotide


<220>
<221> modified_base
<222> (14)..(14)
<223> inosine 

<400> 43
ccgtacaact ggcnt                                                      15


<210> 44
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic 
      oligonucleotide

<400> 44
atgactggtg gacagcaaat gggtaaatta atacgactca ctatagggtt                50


<210> 45
<211> 24
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic 
      oligonucleotide

<400> 45
ccctatagtg agtcgtatta attt                                            24


<210> 46
<211> 25
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic 
      oligonucleotide

<400> 46
acccattttg ctgtccacca gttac                                           25


<210> 47
<211> 34
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic 
      oligonucleotide


<220>
<221> modified_base
<222> (32)..(32)
<223> inosine 

<400> 47
gttttcccag tcacgacgtt gtaaaacgac gncc                                 34


<210> 48
<211> 23
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic 
      oligonucleotide


<220>
<221> modified_base
<222> (22)..(22)
<223> inosine 

<400> 48
tcaaagaagt attgctacaa cng                                             23


<210> 49
<211> 34
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic 
      oligonucleotide

<400> 49
gttttcccag tcacgacgtt gtaaaacgac ggcc                                 34


<210> 50
<211> 23
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic 
      oligonucleotide

<400> 50
tcaaagaagt attgctacaa cgg                                             23


<210> 51
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic 
      oligonucleotide


<220>
<221> modified_base
<222> (32)..(32)
<223> inosine 

<400> 51
gcagatgaag aaaaggttct tgagattatt cnt                                  33


<210> 52
<211> 51
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic 
      oligonucleotide

<220>
<221> modified_base
<222> (51)..(51)
<223> dideoxycytidine 

<400> 52
aaattaatac gactcactat agggttgaag aattaacaga agtaaaagag c              51


<210> 53
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic 
      oligonucleotide


<220>
<221> modified_base
<222> (18)..(18)
<223> inosine

<400> 53
tgctgctggc tgaccctna                                                   18


<210> 54
<211> 112
<212> DNA
<213> Bacillus cereus

<400> 54
caatagactc cataccacca attgtaattt ctgtacgtct cttatcattg aagcgctctt     60

ttacttctgt taattcttca cgaataatct caagaacctt ttcttcatct gc            112



<210> 55
<211> 34
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic 
      oligonucleotide


<220>
<221> modified_base
<222> (33)..(33)
<223> inosine

<400> 55
caatagactc cataccacca attgtaattt ctnt                                 34


