                         SEQUENCE LISTING

<110>  Integrated Plant Genetics, Inc.
 
<120>  USE OF ESTERASE GENES AS SELECTABLE MARKERS FOR TRANSFORMING 
       PLANT CELLS

<130>  INTE-005-01WO

<150>  US 60/870,400
<151>  2006-12-17

<160>  35    

<170>  PatentIn version 3.4

<210>  1
<211>  20
<212>  DNA
<213>  Bos taurus

<400>  1
ctcagctgca tacgccttcc                                                 20


<210>  2
<211>  21
<212>  DNA
<213>  Bos taurus

<400>  2
acaggtcatt gtcagcactc c                                               21


<210>  3
<211>  39
<212>  DNA
<213>  Bos taurus

<400>  3
atgcccatgg aacatatgat gtggtggcta cttgtaaca                            39


<210>  4
<211>  40
<212>  DNA
<213>  Bos taurus

<400>  4
gcatcccggg ctagagctcc tttttgtctt cggccatcaa                           40


<210>  5
<211>  399
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Bovine pregastric esterase cloned by IPG, translation product, 
       with native leader and two amino acids added to form an ER 
       retention signal sequence

<400>  5

Met Trp Trp Leu Leu Val Thr Val Cys Phe Ile His Met Ser Gly Asn 
1               5                   10                  15      


Ala Phe Cys Phe Leu Gly Lys Ile Ala Lys Asn Pro Glu Ala Ser Met 
            20                  25                  30          


Asn Val Ser Gln Met Ile Ser Tyr Trp Gly Tyr Pro Ser Glu Met His 
        35                  40                  45              


Lys Val Ile Thr Ala Asp Gly Tyr Ile Leu Gln Val Tyr Arg Ile Pro 
    50                  55                  60                  


His Gly Lys Asn Asn Ala Asn His Leu Gly Gln Arg Pro Val Val Phe 
65                  70                  75                  80  


Leu Gln His Gly Leu Leu Gly Ser Ala Thr Asn Trp Ile Ser Asn Leu 
                85                  90                  95      


Pro Lys Asn Ser Leu Gly Phe Leu Leu Ala Asp Ala Gly Tyr Asp Val 
            100                 105                 110         


Trp Leu Gly Asn Ser Arg Gly Asn Thr Trp Ala Gln Glu His Leu Tyr 
        115                 120                 125             


Tyr Ser Pro Asp Ser Pro Glu Phe Trp Ala Phe Ser Phe Asp Glu Met 
    130                 135                 140                 


Ala Glu Tyr Asp Leu Pro Ser Thr Ile Asp Phe Ile Leu Arg Arg Thr 
145                 150                 155                 160 


Gly Gln Lys Lys Leu His Tyr Val Gly His Ser Gln Gly Thr Thr Ile 
                165                 170                 175     


Gly Phe Ile Ala Phe Ser Thr Asn Pro Thr Leu Ala Glu Lys Ile Lys 
            180                 185                 190         


Val Phe Tyr Ala Leu Ala Pro Val Ala Thr Val Lys Tyr Thr Lys Ser 
        195                 200                 205             


Leu Phe Asn Lys Leu Ala Leu Ile Pro His Phe Leu Phe Lys Ile Ile 
    210                 215                 220                 


Phe Gly Asp Lys Met Phe Tyr Pro His Thr Phe Leu Glu Gln Phe Leu 
225                 230                 235                 240 


Gly Val Glu Met Cys Ser Arg Glu Thr Leu Asp Val Leu Cys Lys Asn 
                245                 250                 255     


Ala Leu Phe Ala Ile Thr Gly Val Asp Asn Lys Asn Phe Asn Met Ser 
            260                 265                 270         


Arg Leu Asp Val Tyr Ile Ala His Asn Pro Ala Gly Thr Ser Val Gln 
        275                 280                 285             


Asn Thr Leu His Trp Arg Gln Ala Val Lys Ser Gly Lys Phe Gln Ala 
    290                 295                 300                 


Phe Asp Trp Gly Ala Pro Tyr Gln Asn Leu Met His Tyr His Gln Pro 
305                 310                 315                 320 


Thr Pro Pro Ile Tyr Asn Leu Thr Ala Met Asn Val Pro Ile Ala Val 
                325                 330                 335     


Trp Ser Ala Asp Asn Asp Leu Leu Ala Asp Pro Gln Asp Val Asp Leu 
            340                 345                 350         


Leu Leu Ser Lys Leu Ser Asn Leu Ile Tyr His Lys Glu Ile Pro Asn 
        355                 360                 365             


Tyr Asn His Leu Asp Phe Ile Trp Ala Met Asp Ala Pro Gln Glu Val 
    370                 375                 380                 


Tyr Asn Glu Ile Val Ser Leu Met Ala Glu Asp Lys Lys Glu Leu 
385                 390                 395                 


<210>  6
<211>  31
<212>  DNA
<213>  Caenorhabditis elegans

<400>  6
cagctgcata cgccgaaaat gtcaccactc c                                    31


<210>  7
<211>  21
<212>  DNA
<213>  Caenorhabditis elegans

<400>  7
ttacgaaata gtatctggaa g                                               21


<210>  8
<211>  591
<212>  PRT
<213>  Caenorhabditis elegans

<400>  8

Met Lys Ser Leu Leu Leu Leu Phe Leu Leu Phe Leu His Thr Leu Cys 
1               5                   10                  15      


Glu Asn Val Thr Thr Pro Asn Ser Glu Glu Asp Thr Asp Met Thr Ala 
            20                  25                  30          


Thr Pro Ser Thr Ile Thr Pro Leu Leu Ser Thr Ala Gln Asn Pro Ser 
        35                  40                  45              


Leu Pro Lys Thr Ser Lys Leu Pro Leu Ala Leu Lys Thr Pro Val Pro 
    50                  55                  60                  


Thr Phe Pro Phe Ser Ser Leu Ala Thr Ser Asp Trp Leu Ser Ser Met 
65                  70                  75                  80  


Pro Thr Ile Gln Thr Leu Leu Pro Pro Pro Leu Pro Leu Thr Thr Leu 
                85                  90                  95      


Glu Val Pro Glu Asn Glu Asn Phe Gly Phe Thr Ser Leu Ala Pro Leu 
            100                 105                 110         


Trp Thr Leu Pro Thr Gln Pro Pro Ala Trp Ser Pro Met Leu Asp Ser 
        115                 120                 125             


Pro Ile Lys Pro Ile Gln Asn Ser Met Phe Pro Thr Phe Pro Thr Met 
    130                 135                 140                 


Pro Thr Leu Pro Thr Leu Pro Thr Leu Ala Pro Phe Thr Phe Pro Thr 
145                 150                 155                 160 


Leu Pro Pro Pro Thr Thr Met Lys Pro Leu Asn Ile Thr Ile Asp Pro 
                165                 170                 175     


Glu Ala Leu Met Asp Val Pro Glu Ile Ile Thr His Trp Gly Tyr Pro 
            180                 185                 190         


Val Glu Thr His Lys Val Val Thr Val Asp Gly Tyr Ile Leu Thr Leu 
        195                 200                 205             


His Arg Ile Pro His Ser Lys Asn Glu Thr Ser Lys Ser Ala Ser Lys 
    210                 215                 220                 


Thr Pro Lys Pro Val Val Phe Leu Gln His Gly Leu Leu Cys Thr Ser 
225                 230                 235                 240 


Ser Ile Trp Leu Leu Asn Leu Pro Arg Gln Ser Ala Gly Tyr Ile Phe 
                245                 250                 255     


Ala Asp Gln Gly Tyr Asp Val Trp Leu Gly Asn Met Arg Gly Asn Thr 
            260                 265                 270         


Tyr Ser Lys Glu His Thr Arg Met Thr Ser Ala Asp Arg Arg Phe Trp 
        275                 280                 285             


Lys Phe Ser Trp Glu Glu Met Ala Arg Tyr Asp Leu Pro Ala Met Ile 
    290                 295                 300                 


Asn Tyr Ala Leu Lys Thr Thr Lys Arg Gln Asn Leu Tyr Tyr Val Gly 
305                 310                 315                 320 


His Ser Gln Gly Ala Leu Thr Met Phe Ala Lys Met Ser Glu Asp Pro 
                325                 330                 335     


Glu Met Ser Lys Lys Ile Arg Lys Phe Phe Ala Met Ala Pro Val Ala 
            340                 345                 350         


Arg Met Ser His Val Lys Gly Leu Phe Gln Asn Leu Gly Gln Ile Tyr 
        355                 360                 365             


Glu Gln Tyr Asn Leu Val Tyr Gln Val Phe Gly Asp Gly Glu Phe Leu 
    370                 375                 380                 


Thr Asn Asn Ile Phe Thr Lys Leu Leu Thr Asp Ile Phe Cys Asp Gln 
385                 390                 395                 400 


Ala Val Asn Asn Pro Leu Cys Glu Asn Phe Ile Phe Ala Val Ser Gly 
                405                 410                 415     


Pro Asn Ser Asn Gln Phe Asn Asn Ser Arg Ile Gly Ile Tyr Leu Ala 
            420                 425                 430         


His Asn Pro Ala Gly Thr Ser Ser Arg Asn Ile Leu His Phe Ala Gln 
        435                 440                 445             


Met Val Lys Lys Lys Arg Met Ser Arg Phe Asp His Gly Lys Asp Leu 
    450                 455                 460                 


Asn Leu Lys Ile Tyr Gly Ala Pro Ser Pro Pro Glu Tyr Asp Ile Arg 
465                 470                 475                 480 


Lys Ile Asn Ser Ser Ile Tyr Leu Phe Tyr Ser Asp Phe Asp Trp Leu 
                485                 490                 495     


Ala Asn Pro Lys Asp Val Glu Gly Phe Leu Ile Pro Met Leu Pro Ser 
            500                 505                 510         


Lys Thr Leu Lys Lys Ala Thr Lys Leu Arg Asp Phe Asn His Asn Asp 
        515                 520                 525             


Phe Leu Trp Gly Met Arg Ala Arg Lys Glu Ile Tyr Asp Lys Ile Ile 
    530                 535                 540                 


Asn Thr Ile Lys Leu Asp Gln Arg Arg Val Lys Leu Gln Asn Ser Met 
545                 550                 555                 560 


Glu Arg Phe Phe Glu Arg Gln Ser Arg Asn Pro Thr Ser Gly Leu Asp 
                565                 570                 575     


Glu Glu Thr Met Met Arg Leu Arg Asn Glu Thr Met Asn Leu Asp 
            580                 585                 590     


<210>  9
<211>  23
<212>  DNA
<213>  Lactococcus lactis

<400>  9
ccatggcagt aatcaatatc gaa                                             23


<210>  10
<211>  24
<212>  DNA
<213>  Lactococcus lactis

<400>  10
tattaactca atcgttcttc ttgc                                            24


<210>  11
<211>  258
<212>  PRT
<213>  Lactococcus lactis

<400>  11

Met Ala Val Ile Asn Ile Glu Tyr Tyr Ser Glu Val Leu Gly Met Asn 
1               5                   10                  15      


Arg Lys Val Asn Val Ile Tyr Pro Glu Ser Ser Lys Val Glu Asp Phe 
            20                  25                  30          


Ser Asn Thr Glu Ile Pro Val Leu Tyr Leu Leu His Gly Met Ser Gly 
        35                  40                  45              


Asn Glu Asn Ser Trp Met Ile Arg Ser Gly Ile Glu Arg Leu Ile Arg 
    50                  55                  60                  


His Thr Asn Leu Ala Ile Val Met Pro Ser Thr Asp Leu Gly Phe Tyr 
65                  70                  75                  80  


Val Asn Thr Thr Tyr Gly Met Asn Tyr Phe Asp Ala Ile Ala Leu Glu 
                85                  90                  95      


Leu Pro Lys Val Ile His Asn Phe Phe Pro Asn Leu Ser Thr Lys Lys 
            100                 105                 110         


Glu Lys Asn Phe Ile Ala Gly Leu Ser Met Gly Gly Tyr Gly Ala Tyr 
        115                 120                 125             


Arg Leu Ala Leu Gly Thr Asp His Phe Ser Tyr Ala Ala Ser Leu Ser 
    130                 135                 140                 


Gly Val Leu Thr Phe Asp Gly Met Glu Glu Asn Phe Lys Glu Asn Pro 
145                 150                 155                 160 


Ala Tyr Trp Gly Gly Ile Phe Gly Asn Trp Glu Thr Phe Lys Gly Ser 
                165                 170                 175     


Asp Asn Glu Ile Leu Ala Leu Ala Asp Arg Lys Asn Glu Glu Arg Pro 
            180                 185                 190         


Lys Leu Tyr Ala Trp Cys Gly Lys Gln Asp Phe Leu Phe Pro Gly Asn 
        195                 200                 205             


Glu Tyr Ala Ile Ala Glu Leu Lys Lys Lys Gly Phe Asp Val Thr Tyr 
    210                 215                 220                 


Glu Ser Ser Asp Gly Val His Glu Trp Tyr Tyr Trp Thr Lys Lys Ile 
225                 230                 235                 240 


Glu Ser Val Leu Gln Trp Leu Pro Ile Asn Tyr Lys Gln Glu Glu Arg 
                245                 250                 255     


Leu Ser 
        


<210>  12
<211>  21
<212>  DNA
<213>  Arabidopsis thaliana 

<400>  12
tcatgagtat ctccggtgct g                                               21


<210>  13
<211>  24
<212>  DNA
<213>  Arabidopsis thaliana 

<400>  13
actagttcaa ccttcgaggc tgag                                            24


<210>  14
<211>  252
<212>  PRT
<213>  Arabidopsis thaliana 

<400>  14

Met Ser Ile Ser Gly Ala Ala Val Gly Ser Gly Arg Asn Leu Arg Arg 
1               5                   10                  15      


Ala Val Glu Phe Gly Lys Thr His Val Val Arg Pro Lys Gly Lys His 
            20                  25                  30          


Gln Ala Thr Ile Val Trp Leu His Gly Leu Gly Asp Asn Gly Ser Ser 
        35                  40                  45              


Trp Ser Gln Leu Leu Glu Thr Leu Pro Leu Pro Asn Ile Lys Trp Ile 
    50                  55                  60                  


Cys Pro Thr Ala Pro Ser Gln Pro Ile Ser Leu Phe Gly Gly Phe Pro 
65                  70                  75                  80  


Ser Thr Ala Trp Phe Asp Val Val Asp Ile Asn Glu Asp Gly Pro Asp 
                85                  90                  95      


Asp Met Glu Gly Leu Asp Val Ala Ala Ala His Val Ala Asn Leu Leu 
            100                 105                 110         


Ser Asn Glu Pro Ala Asp Ile Lys Leu Gly Val Gly Gly Phe Ser Met 
        115                 120                 125             


Gly Ala Ala Thr Ser Leu Tyr Ser Ala Thr Cys Phe Ala Leu Gly Lys 
    130                 135                 140                 


Tyr Gly Asn Gly Asn Pro Tyr Pro Ile Asn Leu Ser Ala Ile Ile Gly 
145                 150                 155                 160 


Leu Ser Gly Trp Leu Pro Cys Ala Lys Thr Leu Ala Gly Lys Leu Glu 
                165                 170                 175     


Glu Glu Gln Ile Lys Asn Arg Ala Ala Ser Leu Pro Ile Val Val Cys 
            180                 185                 190         


His Gly Lys Ala Asp Asp Val Val Pro Phe Lys Phe Gly Glu Lys Ser 
        195                 200                 205             


Ser Gln Ala Leu Leu Ser Asn Gly Phe Lys Lys Val Thr Phe Lys Pro 
    210                 215                 220                 


Tyr Ser Ala Leu Gly His His Thr Ile Pro Gln Glu Leu Asp Glu Leu 
225                 230                 235                 240 


Cys Ala Trp Leu Thr Ser Thr Leu Ser Leu Glu Gly 
                245                 250         


<210>  15
<211>  18
<212>  DNA
<213>  Arabidopsis thaliana 

<400>  15
cccatggctt cttcactg                                                   18


<210>  16
<211>  24
<212>  DNA
<213>  Arabidopsis thaliana 

<400>  16
actagtccct ttatgtatcc actg                                            24


<210>  17
<211>  384
<212>  PRT
<213>  Arabidopsis thaliana 

<400>  17

Met Ala Ser Ser Leu Lys Lys Leu Ile Ser Ser Phe Leu Leu Val Leu 
1               5                   10                  15      


Tyr Ser Thr Thr Ile Ile Val Ala Ser Ser Glu Ser Arg Cys Arg Arg 
            20                  25                  30          


Phe Lys Ser Ile Ile Ser Phe Gly Asp Ser Ile Ala Asp Thr Gly Asn 
        35                  40                  45              


Tyr Leu His Leu Ser Asp Val Asn His Leu Pro Gln Ser Ala Phe Leu 
    50                  55                  60                  


Pro Tyr Gly Glu Ser Phe Phe His Pro Pro Ser Gly Arg Ala Ser Asn 
65                  70                  75                  80  


Gly Arg Leu Ile Ile Asp Phe Ile Ala Glu Phe Leu Gly Leu Pro Tyr 
                85                  90                  95      


Val Pro Pro Tyr Phe Gly Ser Gln Asn Val Ser Phe Glu Gln Gly Ile 
            100                 105                 110         


Asn Phe Ala Val Tyr Gly Ala Thr Ala Leu Asp Arg Ala Phe Leu Leu 
        115                 120                 125             


Gly Lys Gly Ile Glu Ser Asp Phe Thr Asn Val Ser Leu Ser Val Gln 
    130                 135                 140                 


Leu Asp Thr Phe Lys Gln Ile Leu Pro Asn Leu Cys Ala Ser Ser Thr 
145                 150                 155                 160 


Arg Asp Cys Lys Glu Met Leu Gly Asp Ser Leu Ile Leu Met Gly Glu 
                165                 170                 175     


Ile Gly Gly Asn Asp Tyr Asn Tyr Pro Phe Phe Glu Gly Lys Ser Ile 
            180                 185                 190         


Asn Glu Ile Lys Glu Leu Val Pro Leu Ile Val Lys Ala Ile Ser Ser 
        195                 200                 205             


Ala Ile Val Asp Leu Ile Asp Leu Gly Gly Lys Thr Phe Leu Val Pro 
    210                 215                 220                 


Gly Gly Phe Pro Thr Gly Cys Ser Ala Ala Tyr Leu Thr Leu Phe Gln 
225                 230                 235                 240 


Thr Val Ala Glu Lys Asp Gln Asp Pro Leu Thr Gly Cys Tyr Pro Leu 
                245                 250                 255     


Leu Asn Glu Phe Gly Glu His His Asn Glu Gln Leu Lys Thr Glu Leu 
            260                 265                 270         


Lys Arg Leu Gln Lys Phe Tyr Pro His Val Asn Ile Ile Tyr Ala Asp 
        275                 280                 285             


Tyr His Asn Ser Leu Tyr Arg Phe Tyr Gln Glu Pro Ala Lys Tyr Gly 
    290                 295                 300                 


Phe Lys Asn Lys Pro Leu Ala Ala Cys Cys Gly Val Gly Gly Lys Tyr 
305                 310                 315                 320 


Asn Phe Thr Ile Gly Lys Glu Cys Gly Tyr Glu Gly Val Asn Tyr Cys 
                325                 330                 335     


Gln Asn Pro Ser Glu Tyr Val Asn Trp Asp Gly Tyr His Leu Thr Glu 
            340                 345                 350         


Ala Ala Tyr Gln Lys Met Thr Glu Gly Ile Leu Asn Gly Pro Tyr Ala 
        355                 360                 365             


Thr Pro Ala Phe Asp Trp Ser Cys Leu Gly Ser Gly Thr Val Asp Thr 
    370                 375                 380                 


<210>  18
<211>  404
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Bovine pregastric esterase cloned by IPG with native leader 
       replaced by the citrus P12 leader and two amino acids added to 
       form an ER retention signal sequence

<400>  18

Met Gly Val Gly Thr Lys Val Leu Val Ile Thr Thr Met Ala Ile Cys 
1               5                   10                  15      


Leu Ile Ser Ser Ala Ala Tyr Ala Phe Leu Gly Lys Ile Ala Lys Asn 
            20                  25                  30          


Pro Glu Ala Ser Met Asn Val Ser Gln Met Ile Ser Tyr Trp Gly Tyr 
        35                  40                  45              


Pro Ser Glu Met His Lys Val Ile Thr Ala Asp Gly Tyr Ile Leu Gln 
    50                  55                  60                  


Val Tyr Arg Ile Pro His Gly Lys Asn Asn Ala Asn His Leu Gly Gln 
65                  70                  75                  80  


Arg Pro Val Val Phe Leu Gln His Gly Leu Leu Gly Ser Ala Thr Asn 
                85                  90                  95      


Trp Ile Ser Asn Leu Pro Lys Asn Ser Leu Gly Phe Leu Leu Ala Asp 
            100                 105                 110         


Ala Gly Tyr Asp Val Trp Leu Gly Asn Ser Arg Gly Asn Thr Trp Ala 
        115                 120                 125             


Gln Glu His Leu Tyr Tyr Ser Pro Asp Ser Pro Glu Phe Trp Ala Phe 
    130                 135                 140                 


Ser Phe Asp Glu Met Ala Glu Tyr Asp Leu Pro Ser Thr Ile Asp Phe 
145                 150                 155                 160 


Ile Leu Arg Arg Thr Gly Gln Lys Lys Leu His Tyr Val Gly His Ser 
                165                 170                 175     


Gln Gly Thr Thr Ile Gly Phe Ile Ala Phe Ser Thr Asn Pro Thr Leu 
            180                 185                 190         


Ala Glu Lys Ile Lys Val Phe Tyr Ala Leu Ala Pro Val Ala Thr Val 
        195                 200                 205             


Lys Tyr Thr Lys Ser Leu Phe Asn Lys Leu Ala Leu Ile Pro His Phe 
    210                 215                 220                 


Leu Phe Lys Ile Ile Phe Gly Asp Lys Met Phe Tyr Pro His Thr Phe 
225                 230                 235                 240 


Leu Glu Gln Phe Leu Gly Val Glu Met Cys Ser Arg Glu Thr Leu Asp 
                245                 250                 255     


Val Leu Cys Lys Asn Ala Leu Phe Ala Ile Thr Gly Val Asp Asn Lys 
            260                 265                 270         


Asn Phe Asn Met Ser Arg Leu Asp Val Tyr Ile Ala His Asn Pro Ala 
        275                 280                 285             


Gly Thr Ser Val Gln Asn Thr Leu His Trp Arg Gln Ala Val Lys Ser 
    290                 295                 300                 


Gly Lys Phe Gln Ala Phe Asp Trp Gly Ala Pro Tyr Gln Asn Leu Met 
305                 310                 315                 320 


His Tyr His Gln Pro Thr Pro Pro Ile Tyr Asn Leu Thr Ala Met Asn 
                325                 330                 335     


Val Pro Ile Ala Val Trp Ser Ala Asp Asn Asp Leu Leu Ala Asp Pro 
            340                 345                 350         


Gln Asp Val Asp Leu Leu Leu Ser Lys Leu Ser Asn Leu Ile Tyr His 
        355                 360                 365             


Lys Glu Ile Pro Asn Tyr Asn His Leu Asp Phe Ile Trp Ala Met Asp 
    370                 375                 380                 


Ala Pro Gln Glu Val Tyr Asn Glu Ile Val Ser Leu Met Ala Glu Asp 
385                 390                 395                 400 


Lys Lys Glu Leu 
                


<210>  19
<211>  1215
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Bovine pregastric esterase with P12 leader coding sequence and ER
       retention signal sequence added

<400>  19
atgggtgtag gcacaaaagt tctggtgatc acaactatgg ccatatgcct aattagctca     60

gctgcatacg ccttccttgg aaaaattgct aagaaccctg aagccagtat gaatgttagt    120

cagatgattt cctactgggg ctacccaagt gagatgcata aagttataac tgcggatggt    180

tatatccttc aggtctatcg gattcctcat ggaaagaata atgctaatca tttaggtcag    240

agacctgttg tgtttctgca gcatggtctt cttggatcag ccacaaactg gatttccaac    300

ctgcccaaga acagcctggg cttcctcctg gcagatgctg gttatgacgt gtggctgggg    360

aacagcagag gaaacacctg ggcccaggaa catttatact attcaccaga ctccccggaa    420

ttctgggctt tcagctttga tgaaatggct gaatatgacc ttccatctac aattgatttc    480

atcttaagga gaacaggaca gaagaagcta cactatgttg gccattccca aggcaccacc    540

attggtttta tcgccttttc taccaatccc acattggctg aaaaaatcaa agtcttctat    600

gcattagccc cagttgccac agtgaagtac accaagagcc tgtttaacaa acttgcactt    660

attcctcact tcctcttcaa gattatattt ggtgacaaaa tgttctaccc acacactttt    720

ttggaacaat ttcttggtgt tgaaatgtgc tcccgtgaga cactggatgt cctttgtaag    780

aatgccttgt ttgccattac tggagttgac aataaaaact tcaacatgag tcgcttagat    840

gtgtatatag cacataatcc agcaggaact tctgttcaaa acaccctcca ctggagacag    900

gctgttaagt ctgggaaatt ccaagctttt gactggggag ccccatatca gaacctaatg    960

cattatcatc agcccacacc tcccatctac aatttaacag ccatgaatgt cccaattgca   1020

gtatggagtg ctgacaatga cctgttggct gaccctcagg atgttgacct tctgctttca   1080

aaactctcta atctcattta ccacaaggaa attccaaatt acaatcactt ggactttatc   1140

tgggcaatgg atgcacctca agaagtttac aatgaaattg tttctttgat ggccgaagac   1200

aaaaaggagc tctag                                                    1215


<210>  20
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA primer used to amplify and reclone bovine PGE gene and remove
       ER retention signal

<400>  20
cgaacggctg ttaagtctgg gaa                                             23


<210>  21
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA primer used to amplify and reclone bovine PGE gene and remove
       ER retention signal

<400>  21
ccaactagta ttactttttg tcttcggcca tc                                   32


<210>  22
<211>  402
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Bovine pregastric esterase with native leader replaced by the 
       citrus P12 leader and without ER retention signal

<400>  22

Met Gly Val Gly Thr Lys Val Leu Val Ile Thr Thr Met Ala Ile Cys 
1               5                   10                  15      


Leu Ile Ser Ser Ala Ala Tyr Ala Phe Leu Gly Lys Ile Ala Lys Asn 
            20                  25                  30          


Pro Glu Ala Ser Met Asn Val Ser Gln Met Ile Ser Tyr Trp Gly Tyr 
        35                  40                  45              


Pro Ser Glu Met His Lys Val Ile Thr Ala Asp Gly Tyr Ile Leu Gln 
    50                  55                  60                  


Val Tyr Arg Ile Pro His Gly Lys Asn Asn Ala Asn His Leu Gly Gln 
65                  70                  75                  80  


Arg Pro Val Val Phe Leu Gln His Gly Leu Leu Gly Ser Ala Thr Asn 
                85                  90                  95      


Trp Ile Ser Asn Leu Pro Lys Asn Ser Leu Gly Phe Leu Leu Ala Asp 
            100                 105                 110         


Ala Gly Tyr Asp Val Trp Leu Gly Asn Ser Arg Gly Asn Thr Trp Ala 
        115                 120                 125             


Gln Glu His Leu Tyr Tyr Ser Pro Asp Ser Pro Glu Phe Trp Ala Phe 
    130                 135                 140                 


Ser Phe Asp Glu Met Ala Glu Tyr Asp Leu Pro Ser Thr Ile Asp Phe 
145                 150                 155                 160 


Ile Leu Arg Arg Thr Gly Gln Lys Lys Leu His Tyr Val Gly His Ser 
                165                 170                 175     


Gln Gly Thr Thr Ile Gly Phe Ile Ala Phe Ser Thr Asn Pro Thr Leu 
            180                 185                 190         


Ala Glu Lys Ile Lys Val Phe Tyr Ala Leu Ala Pro Val Ala Thr Val 
        195                 200                 205             


Lys Tyr Thr Lys Ser Leu Phe Asn Lys Leu Ala Leu Ile Pro His Phe 
    210                 215                 220                 


Leu Phe Lys Ile Ile Phe Gly Asp Lys Met Phe Tyr Pro His Thr Phe 
225                 230                 235                 240 


Leu Glu Gln Phe Leu Gly Val Glu Met Cys Ser Arg Glu Thr Leu Asp 
                245                 250                 255     


Val Leu Cys Lys Asn Ala Leu Phe Ala Ile Thr Gly Val Asp Asn Lys 
            260                 265                 270         


Asn Phe Asn Met Ser Arg Leu Asp Val Tyr Ile Ala His Asn Pro Ala 
        275                 280                 285             


Gly Thr Ser Val Gln Asn Thr Leu His Trp Arg Gln Ala Val Lys Ser 
    290                 295                 300                 


Gly Lys Phe Gln Ala Phe Asp Trp Gly Ala Pro Tyr Gln Asn Leu Met 
305                 310                 315                 320 


His Tyr His Gln Pro Thr Pro Pro Ile Tyr Asn Leu Thr Ala Met Asn 
                325                 330                 335     


Val Pro Ile Ala Val Trp Ser Ala Asp Asn Asp Leu Leu Ala Asp Pro 
            340                 345                 350         


Gln Asp Val Asp Leu Leu Leu Ser Lys Leu Ser Asn Leu Ile Tyr His 
        355                 360                 365             


Lys Glu Ile Pro Asn Tyr Asn His Leu Asp Phe Ile Trp Ala Met Asp 
    370                 375                 380                 


Ala Pro Gln Glu Val Tyr Asn Glu Ile Val Ser Leu Met Ala Glu Asp 
385                 390                 395                 400 


Lys Lys 
        


<210>  23
<211>  599
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Nematode triglyceride lipase-cholesterol esterase cloned by IPG, 
       translation product, with native leader replaced by the citrus 
       P12 leader

<400>  23

Met Gly Val Gly Thr Lys Val Leu Val Ile Thr Thr Met Ala Ile Cys 
1               5                   10                  15      


Leu Ile Ser Ser Ala Ala Tyr Ala Glu Asn Val Thr Thr Pro Asn Ser 
            20                  25                  30          


Glu Glu Asp Thr Asp Met Thr Ala Thr Pro Ser Thr Ile Thr Pro Leu 
        35                  40                  45              


Leu Ser Thr Ala Gln Asn Pro Ser Leu Pro Lys Thr Ser Lys Leu Pro 
    50                  55                  60                  


Leu Ala Leu Lys Thr Pro Val Pro Thr Phe Pro Phe Ser Ser Leu Ala 
65                  70                  75                  80  


Thr Ser Asp Trp Leu Ser Ser Met Pro Thr Ile Gln Thr Leu Leu Pro 
                85                  90                  95      


Pro Pro Leu Pro Leu Thr Thr Leu Glu Val Pro Glu Asn Glu Asn Phe 
            100                 105                 110         


Gly Phe Thr Ser Leu Ala Pro Leu Trp Thr Leu Pro Thr Gln Pro Pro 
        115                 120                 125             


Ala Trp Ser Pro Met Leu Asp Ser Pro Ile Lys Pro Ile Gln Asn Ser 
    130                 135                 140                 


Met Phe Pro Thr Phe Pro Thr Met Pro Thr Leu Pro Thr Leu Pro Thr 
145                 150                 155                 160 


Leu Ala Pro Phe Thr Phe Pro Thr Leu Pro Pro Pro Thr Thr Met Lys 
                165                 170                 175     


Pro Leu Asn Ile Thr Ile Asp Pro Glu Ala Leu Met Asp Val Pro Glu 
            180                 185                 190         


Ile Ile Thr His Trp Gly Tyr Pro Val Glu Thr His Lys Val Val Thr 
        195                 200                 205             


Val Asp Gly Tyr Ile Leu Thr Leu His Arg Ile Pro His Ser Lys Asn 
    210                 215                 220                 


Glu Thr Ser Lys Ser Ala Ser Lys Thr Pro Lys Pro Val Val Phe Leu 
225                 230                 235                 240 


Gln His Gly Leu Leu Cys Thr Ser Ser Ile Trp Leu Leu Asn Leu Pro 
                245                 250                 255     


Arg Gln Ser Ala Gly Tyr Ile Phe Ala Asp Gln Gly Tyr Asp Val Trp 
            260                 265                 270         


Leu Gly Asn Met Arg Gly Asn Thr Tyr Ser Lys Glu His Thr Arg Met 
        275                 280                 285             


Thr Ser Ala Asp Arg Arg Phe Trp Lys Phe Ser Trp Glu Glu Met Ala 
    290                 295                 300                 


Arg Tyr Asp Leu Pro Ala Met Ile Asn Tyr Ala Leu Lys Thr Thr Lys 
305                 310                 315                 320 


Arg Gln Asn Leu Tyr Tyr Val Gly His Ser Gln Gly Ala Leu Thr Met 
                325                 330                 335     


Phe Ala Lys Met Ser Glu Asp Pro Glu Met Ser Lys Lys Ile Arg Lys 
            340                 345                 350         


Phe Phe Ala Met Ala Pro Val Ala Arg Met Ser His Val Lys Gly Leu 
        355                 360                 365             


Phe Gln Asn Leu Gly Gln Ile Tyr Glu Gln Tyr Asn Leu Val Tyr Gln 
    370                 375                 380                 


Val Phe Gly Asp Gly Glu Phe Leu Thr Asn Asn Ile Phe Thr Lys Leu 
385                 390                 395                 400 


Leu Thr Asp Ile Phe Cys Asp Gln Ala Val Asn Asn Pro Leu Cys Glu 
                405                 410                 415     


Asn Phe Ile Phe Ala Val Ser Gly Pro Asn Ser Asn Gln Phe Asn Asn 
            420                 425                 430         


Ser Arg Ile Gly Ile Tyr Leu Ala His Asn Pro Ala Gly Thr Ser Ser 
        435                 440                 445             


Arg Asn Ile Leu His Phe Ala Gln Met Val Lys Lys Lys Arg Met Ser 
    450                 455                 460                 


Arg Phe Asp His Gly Lys Asp Leu Asn Leu Lys Ile Tyr Gly Ala Pro 
465                 470                 475                 480 


Ser Pro Pro Glu Tyr Asp Ile Arg Lys Ile Asn Ser Ser Ile Tyr Leu 
                485                 490                 495     


Phe Tyr Ser Asp Phe Asp Trp Leu Ala Asn Pro Lys Asp Val Glu Gly 
            500                 505                 510         


Phe Leu Ile Pro Met Leu Pro Ser Lys Thr Leu Lys Lys Ala Thr Lys 
        515                 520                 525             


Leu Arg Asp Phe Asn His Asn Asp Phe Leu Trp Gly Met Arg Ala Arg 
    530                 535                 540                 


Lys Glu Ile Tyr Asp Lys Ile Ile Asn Thr Ile Lys Leu Asp Gln Arg 
545                 550                 555                 560 


Arg Val Lys Leu Gln Asn Ser Met Glu Arg Phe Phe Glu Arg Gln Ser 
                565                 570                 575     


Arg Asn Pro Thr Ser Gly Leu Asp Glu Glu Thr Met Met Arg Leu Arg 
            580                 585                 590         


Asn Glu Thr Met Asn Leu Asp 
        595                 


<210>  24
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA primer for use in amplifying and modifying the bacterial 
       tributyrin esterase gene encoding the native bacterial esterase 
       so as to contain part of the P12 leader

<400>  24
cagctgcata cgccatggca gtaatcaata tc                                   32


<210>  25
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA primer for use in amplifying and modifying the bacterial 
       tributyrin esterase gene encoding the native bacterial esterase 
       so as to contain part of the P12 leader

<400>  25
tattaactca atcgttcttc ttgc                                            24


<210>  26
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA primer for use in amplifying and modifying the plant 
       carboxylesterase gene encoding the native plant carboxylesterase 
       so as to contain part of the P12 leader

<400>  26
cagctgcata cgccatgagt atctccggtg ctg                                  33


<210>  27
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA primer for use in amplifying and modifying the plant 
       carboxylesterase gene encoding the native plant carboxylesterase 
       so as to contain part of the P12 leader

<400>  27
actagttcaa ccttcgaggc tgag                                            24


<210>  28
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA primer for use in amplifying and modifying the plant 
       carboxylesterase gene encoding the native plant carboxylesterase 
       so as to contain part of the P12 leader

<400>  28
cagctgcata cgccatggct tcttcactg                                       29


<210>  29
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA primer for use in amplifying and modifying the plant 
       carboxylesterase gene encoding the native plant carboxylesterase 
       so as to contain part of the P12 leader

<400>  29
actagtccct ttatgtatcc actg                                            24


<210>  30
<211>  20
<212>  DNA
<213>  Caenorhabditis elegans

<400>  30
ttgcgccact atggacattg                                                 20


<210>  31
<211>  20
<212>  DNA
<213>  Caenorhabditis elegans

<400>  31
gtgagtgcac cttgtgaatg                                                 20


<210>  32
<211>  22
<212>  DNA
<213>  Dictyostelium discoideum 

<400>  32
ttactactgc cgtaaacatt cc                                              22


<210>  33
<211>  23
<212>  DNA
<213>  Dictyostelium discoideum 

<400>  33
ttaaacatat ccaccttggt tac                                             23


<210>  34
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA primer for use in amplifying and modifying the amoeba 
       acyloxyacyl hydrolase gene so as to contain part of the P12 
       leader

<400>  34
cagctgcata cgccgtaaac attccag                                         27


<210>  35
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA primer for use in amplifying and modifying the amoeba 
       acyloxyacyl hydrolase gene so as to contain part of the P12 
       leader

<400>  35
actagtttaa acatatccac cttggttac                                       29


