
                                SEQUENCE LISTING

<110> U.S. Smokeless Tobacco Company

<120> TOBACCO PLANTS HAVING A MUTATION IN A
  NICOTINE DEMETHYLASE GENE
  

<130> 20210-075WO1

<150> US 11/611,782       
<151> 2006-12-15  

<160> 11

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 2552
<212> DNA
<213> Nicotiana tabacum

<400> 1
atgctttctc ccatagaagc cattgtagga ctagtaacct tcacatttct cttcttcttc 60
ctatggacaa aaaaatctca aaaaccttca aaacccttac caccgaaaat ccccggagga 120
tggccggtaa tcggccatct tttccacttc aatgacgacg gcgacgaccg tccattagct 180
cgaaaactcg gagacttagc tgacaaatac ggccccgttt tcacttttcg gctaggcctt 240
ccccttgtct tagttgtaag cagttacgaa gctgtaaaag actgtttctc tacaaatgac 300
gccatttttt ccaatcgtcc agcttttctt tacggcgatt accttggcta caataatgcc 360
atgctatttt tggccaatta cggaccttac tggcgaaaaa atcgaaaatt agttattcag 420
gaagttctct ccgctagtcg tctcgaaaaa ttcaaacacg tgagatttgc aagaattcaa 480
gcgagcatta agaatttata tactcgaatt gatggaaatt cgagtacgat aaatttaact 540
gattggttag aagaattgaa ttttggtctg atcgtgaaga tgatcgctgg aaaaaattat 600
gaatccggta aaggagatga acaagtggag agatttaaga aagcgtttaa ggattttatg 660
attttatcaa tggagtttgt gttatgggat gcatttccaa ttccattatt taaatgggtg 720
gattttcaag ggcatgttaa ggctatgaaa aggactttta aagatataga ttctgttttt 780
cagaattggt tagaggaaca tattaataaa agagaaaaaa tggaggttaa tgcagaaggg 840
aatgaacaag atttcattga tgtggtgctt tcaaaaatga gtaatgaata tcttggtgaa 900
ggttactctc gtgatactgt cattaaagca acggtgtttg taagttcatc tgtcattttt 960
catttattca cttttatttt gaggagcaga catgttaata ataatttgga gcaactgtaa 1020
agttatctat gtgtacaggt tcgagcctca ggtgcaacca ctaatgcttg tattagatta 1080
tgttgtctgc atcatacccc taattggagt gtggctcttc ccgaaccctg caatgctgga 1140
tgctggatgc tttatgtatc agactgacct ttttgttaaa ctatctaaat actaaggatg 1200
atttaataaa aatatagaat ggtaaacaga aaaagatgag attatttttg gggctatatg 1260
gattcgcccg ggctttggga ggtaaaacgg tatctaccag ttgagacttt actccagaac 1320
tttatctcga gagctctgaa taaaaatgaa atagtattta ccactccaaa atctttgatg 1380
gtaaaaagat gagatataac ctcttataat tgattgaacc acgttgatag aataaaactt 1440
ctttactccc attcagcata agaaaaatga aaccaaacgg aattcttctc ttttttaggg 1500
ggaaattcct taattgcttg ttgaatatag attcatgtcg ttattctatt tttaataatg 1560
atgaaaatca atatagtcaa agttaatact tatgtcattt ggtttgcgga caagttatat 1620
tggaactata taatacgtct attatagaat agtgattatt tagaggatat acattttttt 1680
tggataaata tttgatttat tggattaaaa atagaatata caggtaaggt ctaaaacgtg 1740
tgtttgcttt tacactaaat aaacttgacc tcgtacaatt ctaagaaaat atttgaaata 1800
aatgaattat tttattgtta atcaattaaa aaaatcatag tatagatgag atgtgtgcat 1860
acttgacaat aactatacta actaaaacaa ggtatgtgaa taattgatat tcctttttta 1920
attattcttt tttccagagt ttggtcttgg atgcagcaga cacagttgct cttcacataa 1980
attggggaat ggcattattg ataaacaatc aaaaggcctt gacgaaagca caagaagaga 2040
tagacacaaa agttggtaag gacagatggg tagaagagag tgatattaag gatttggtat 2100
acctccaagc tattgttaaa gaagtgttac gattatatcc accaggacct ttgttagtac 2160
cacacgaaaa tgtagaagat tgtgttgtta gtggatatca cattcctaaa gggacaagat 2220
tattcgcaaa cgtcatgaaa ctgcaacgtg atcctaaact ctggtctgat cctgatactt 2280
tcgatccaga gagattcatt gctactgata ttgactttcg tggtcagtac tataagtata 2340
tcccgtttgg ttctggaaga cgatcttgtc cagggatgac ttatgcattg caagtggaac 2400
acttaacaat ggcacatttg atccaaggtt tcaattacag aactccaaat gacgagccct 2460
tggatatgaa ggaaggtgca ggcataacta tacgtaaggt aaatcctgtg gaactgataa 2520
tagcgcctcg cctggcacct gagctttatt aa                               2552

<210> 2
<211> 517
<212> PRT
<213> Nicotiana tabacum

<400> 2
Met Leu Ser Pro Ile Glu Ala Ile Val Gly Leu Val Thr Phe Thr Phe
 1               5                  10                  15      
Leu Phe Phe Phe Leu Trp Thr Lys Lys Ser Gln Lys Pro Ser Lys Pro
            20                  25                  30          
Leu Pro Pro Lys Ile Pro Gly Gly Trp Pro Val Ile Gly His Leu Phe
        35                  40                  45              
His Phe Asn Asp Asp Gly Asp Asp Arg Pro Leu Ala Arg Lys Leu Gly
    50                  55                  60                  
Asp Leu Ala Asp Lys Tyr Gly Pro Val Phe Thr Phe Arg Leu Gly Leu
65                  70                  75                  80  
Pro Leu Val Leu Val Val Ser Ser Tyr Glu Ala Val Lys Asp Cys Phe
                85                  90                  95      
Ser Thr Asn Asp Ala Ile Phe Ser Asn Arg Pro Ala Phe Leu Tyr Gly
            100                 105                 110         
Asp Tyr Leu Gly Tyr Asn Asn Ala Met Leu Phe Leu Ala Asn Tyr Gly
        115                 120                 125             
Pro Tyr Trp Arg Lys Asn Arg Lys Leu Val Ile Gln Glu Val Leu Ser
    130                 135                 140                 
Ala Ser Arg Leu Glu Lys Phe Lys His Val Arg Phe Ala Arg Ile Gln
145                 150                 155                 160 
Ala Ser Ile Lys Asn Leu Tyr Thr Arg Ile Asp Gly Asn Ser Ser Thr
                165                 170                 175     
Ile Asn Leu Thr Asp Trp Leu Glu Glu Leu Asn Phe Gly Leu Ile Val
            180                 185                 190         
Lys Met Ile Ala Gly Lys Asn Tyr Glu Ser Gly Lys Gly Asp Glu Gln
        195                 200                 205             
Val Glu Arg Phe Lys Lys Ala Phe Lys Asp Phe Met Ile Leu Ser Met
    210                 215                 220                 
Glu Phe Val Leu Trp Asp Ala Phe Pro Ile Pro Leu Phe Lys Trp Val
225                 230                 235                 240 
Asp Phe Gln Gly His Val Lys Ala Met Lys Arg Thr Phe Lys Asp Ile
                245                 250                 255     
Asp Ser Val Phe Gln Asn Trp Leu Glu Glu His Ile Asn Lys Arg Glu
            260                 265                 270         
Lys Met Glu Val Asn Ala Glu Gly Asn Glu Gln Asp Phe Ile Asp Val
        275                 280                 285             
Val Leu Ser Lys Met Ser Asn Glu Tyr Leu Gly Glu Gly Tyr Ser Arg
    290                 295                 300                 
Asp Thr Val Ile Lys Ala Thr Val Phe Ser Leu Val Leu Asp Ala Ala
305                 310                 315                 320 
Asp Thr Val Ala Leu His Ile Asn Trp Gly Met Ala Leu Leu Ile Asn
                325                 330                 335     
Asn Gln Lys Ala Leu Thr Lys Ala Gln Glu Glu Ile Asp Thr Lys Val
            340                 345                 350         
Gly Lys Asp Arg Trp Val Glu Glu Ser Asp Ile Lys Asp Leu Val Tyr
        355                 360                 365             
Leu Gln Ala Ile Val Lys Glu Val Leu Arg Leu Tyr Pro Pro Gly Pro
    370                 375                 380                 
Leu Leu Val Pro His Glu Asn Val Glu Asp Cys Val Val Ser Gly Tyr
385                 390                 395                 400 
His Ile Pro Lys Gly Thr Arg Leu Phe Ala Asn Val Met Lys Leu Gln
                405                 410                 415     
Arg Asp Pro Lys Leu Trp Ser Asp Pro Asp Thr Phe Asp Pro Glu Arg
            420                 425                 430         
Phe Ile Ala Thr Asp Ile Asp Phe Arg Gly Gln Tyr Tyr Lys Tyr Ile
        435                 440                 445             
Pro Phe Gly Ser Gly Arg Arg Ser Cys Pro Gly Met Thr Tyr Ala Leu
    450                 455                 460                 
Gln Val Glu His Leu Thr Met Ala His Leu Ile Gln Gly Phe Asn Tyr
465                 470                 475                 480 
Arg Thr Pro Asn Asp Glu Pro Leu Asp Met Lys Glu Gly Ala Gly Ile
                485                 490                 495     
Thr Ile Arg Lys Val Asn Pro Val Glu Leu Ile Ile Ala Pro Arg Leu
            500                 505                 510         
Ala Pro Glu Leu Tyr
        515         


<210> 3
<211> 517
<212> PRT
<213> Nicotiana tabacum

<400> 3
Met Leu Ser Pro Ile Glu Ala Ile Val Gly Leu Val Thr Phe Thr Phe
 1               5                  10                  15      
Leu Phe Phe Phe Leu Trp Thr Lys Lys Ser Gln Lys Pro Ser Lys Pro
            20                  25                  30          
Leu Pro Pro Lys Ile Pro Gly Gly Trp Pro Val Ile Gly His Leu Phe
        35                  40                  45              
His Phe Asn Asp Asp Gly Asp Asp Arg Pro Leu Ala Arg Lys Leu Gly
    50                  55                  60                  
Asp Leu Ala Asp Lys Tyr Gly Pro Val Phe Thr Phe Arg Leu Gly Leu
65                  70                  75                  80  
Pro Leu Val Leu Val Val Ser Ser Tyr Glu Ala Val Lys Asp Cys Phe
                85                  90                  95      
Ser Thr Asn Asp Ala Ile Phe Ser Asn Arg Pro Ala Phe Leu Tyr Gly
            100                 105                 110         
Asp Tyr Leu Gly Tyr Asn Asn Ala Met Leu Phe Leu Ala Asn Tyr Gly
        115                 120                 125             
Pro Tyr Trp Arg Lys Asn Arg Lys Leu Val Ile Gln Glu Val Leu Ser
    130                 135                 140                 
Ala Ser Arg Leu Glu Lys Phe Lys His Val Arg Phe Ala Arg Ile Gln
145                 150                 155                 160 
Ala Ser Met Lys Asn Leu Tyr Thr Arg Ile Asp Gly Asn Ser Ser Thr
                165                 170                 175     
Ile Asn Leu Thr Asp Trp Leu Glu Glu Leu Asn Phe Gly Leu Ile Val
            180                 185                 190         
Lys Met Ile Ala Gly Lys Asn Tyr Glu Ser Gly Lys Gly Asp Glu Gln
        195                 200                 205             
Val Glu Arg Phe Lys Lys Ala Phe Lys Asp Phe Met Ile Leu Ser Met
    210                 215                 220                 
Glu Phe Val Leu Trp Asp Ala Phe Pro Ile Pro Leu Phe Lys Trp Val
225                 230                 235                 240 
Asp Phe Gln Gly His Val Lys Ala Met Lys Arg Thr Phe Lys Asp Ile
                245                 250                 255     
Asp Ser Val Phe Gln Asn Trp Leu Glu Glu His Ile Asn Lys Arg Glu
            260                 265                 270         
Lys Met Glu Val Asn Ala Glu Gly Asn Glu Gln Asp Phe Ile Asp Val
        275                 280                 285             
Val Leu Ser Lys Met Ser Asn Glu Tyr Leu Gly Glu Gly Tyr Ser Arg
    290                 295                 300                 
Asp Thr Val Ile Glu Ala Thr Val Phe Ser Leu Val Leu Asp Ala Ala
305                 310                 315                 320 
Asp Thr Val Ala Leu His Ile Asn Trp Gly Met Ala Leu Leu Ile Asn
                325                 330                 335     
Asn Gln Lys Ala Leu Thr Lys Ala Gln Glu Glu Ile Asp Thr Lys Val
            340                 345                 350         
Cys Lys Asp Arg Trp Val Glu Glu Ser Asp Ile Lys Asp Leu Val Tyr
        355                 360                 365             
Leu Gln Ala Ile Val Lys Glu Val Leu Arg Leu Tyr Pro Pro Gly Pro
    370                 375                 380                 
Leu Leu Val Pro His Glu Asn Val Glu Asp Cys Val Val Ser Gly Tyr
385                 390                 395                 400 
His Ile Pro Lys Gly Thr Arg Leu Phe Ala Asn Val Met Lys Leu Gln
                405                 410                 415     
Arg Asp Pro Lys Leu Trp Ser Asp Pro Asp Thr Phe Asp Pro Glu Arg
            420                 425                 430         
Phe Ile Ala Thr Asp Ile Asp Phe Arg Gly Gln Tyr Tyr Lys Tyr Ile
        435                 440                 445             
Pro Phe Gly Pro Gly Arg Arg Ser Cys Pro Gly Met Thr Tyr Ala Leu
    450                 455                 460                 
Gln Val Glu His Leu Thr Met Ala His Leu Ile Gln Gly Phe Asn Tyr
465                 470                 475                 480 
Arg Thr Pro Asn Asp Glu Pro Leu Asp Met Lys Glu Gly Ala Gly Ile
                485                 490                 495     
Thr Ile Arg Lys Val Asn Pro Val Glu Leu Ile Ile Ala Pro Arg Leu
            500                 505                 510         
Ala Pro Glu Leu Tyr
        515         


<210> 4
<211> 517
<212> PRT
<213> Nicotiana tabacum

<400> 4
Met Leu Ser Pro Ile Glu Ala Ile Val Gly Leu Val Thr Phe Thr Phe
 1               5                  10                  15      
Leu Phe Phe Phe Leu Trp Thr Lys Lys Ser Gln Lys Pro Ser Lys Pro
            20                  25                  30          
Leu Pro Pro Lys Ile Pro Gly Gly Trp Pro Val Ile Gly His Leu Phe
        35                  40                  45              
His Phe Asn Asp Asp Gly Asp Asp Arg Pro Leu Ala Arg Lys Leu Gly
    50                  55                  60                  
Asp Leu Ala Asp Lys Tyr Gly Pro Val Phe Thr Phe Arg Leu Gly Leu
65                  70                  75                  80  
Pro Leu Val Leu Val Val Ser Ser Tyr Glu Ala Val Lys Asp Cys Phe
                85                  90                  95      
Ser Thr Asn Asp Ala Ile Phe Ser Asn Arg Pro Ala Phe Leu Tyr Gly
            100                 105                 110         
Asp Tyr Leu Gly Tyr Asn Asn Ala Met Leu Phe Leu Ala Asn Tyr Gly
        115                 120                 125             
Pro Tyr Trp Arg Lys Asn Arg Lys Leu Val Ile Gln Glu Val Leu Ser
    130                 135                 140                 
Ala Ser Arg Leu Glu Lys Phe Lys His Val Arg Phe Ala Arg Ile Gln
145                 150                 155                 160 
Ala Ser Ile Lys Asn Leu Tyr Thr Arg Ile Asp Gly Asn Ser Ser Thr
                165                 170                 175     
Ile Asn Leu Thr Asp Trp Leu Glu Glu Leu Asn Phe Gly Leu Ile Val
            180                 185                 190         
Lys Met Ile Ala Gly Lys Asn Tyr Glu Ser Gly Lys Gly Asp Glu Gln
        195                 200                 205             
Val Glu Arg Phe Lys Lys Ala Phe Lys Asp Phe Met Ile Leu Ser Met
    210                 215                 220                 
Glu Phe Val Leu Trp Asp Ala Phe Pro Ile Pro Leu Phe Lys Trp Val
225                 230                 235                 240 
Asp Phe Gln Gly His Val Lys Ala Met Lys Arg Thr Phe Lys Asp Ile
                245                 250                 255     
Asp Ser Val Phe Gln Asn Trp Leu Glu Glu His Ile Asn Lys Arg Glu
            260                 265                 270         
Lys Met Glu Val Asn Ala Glu Gly Asn Glu Gln Asp Phe Ile Asp Val
        275                 280                 285             
Val Leu Ser Lys Met Ser Asn Glu Tyr Leu Gly Glu Gly Tyr Ser Arg
    290                 295                 300                 
Asp Thr Val Ile Lys Ala Thr Val Phe Ser Leu Val Leu Asp Ala Ala
305                 310                 315                 320 
Asp Thr Val Ala Leu His Ile Asn Trp Gly Met Ala Leu Leu Ile Asn
                325                 330                 335     
Asn Gln Lys Ala Leu Thr Lys Ala Gln Glu Glu Ile Asp Thr Lys Val
            340                 345                 350         
Gly Lys Asp Arg Trp Val Glu Glu Ser Asp Ile Lys Asp Leu Val Tyr
        355                 360                 365             
Leu Gln Ala Ile Val Lys Glu Val Leu Arg Leu Tyr Pro Pro Gly Pro
    370                 375                 380                 
Leu Leu Val Pro His Glu Asn Val Glu Asp Cys Val Val Ser Gly Tyr
385                 390                 395                 400 
His Ile Pro Lys Gly Thr Arg Leu Phe Ala Asn Val Met Lys Leu Leu
                405                 410                 415     
Arg Asp Pro Lys Leu Trp Pro Asp Pro Asp Thr Phe Asp Pro Glu Arg
            420                 425                 430         
Phe Ile Ala Thr Asp Ile Asp Phe Arg Gly Gln Tyr Tyr Lys Tyr Ile
        435                 440                 445             
Pro Phe Gly Ser Gly Arg Arg Ser Cys Pro Gly Met Thr Tyr Ala Leu
    450                 455                 460                 
Gln Val Glu His Leu Thr Met Ala His Leu Ile Gln Gly Phe Asn Tyr
465                 470                 475                 480 
Arg Thr Pro Asn Asp Glu Pro Leu Asp Met Lys Glu Gly Ala Gly Ile
                485                 490                 495     
Thr Ile Arg Lys Val Asn Pro Val Glu Leu Ile Ile Ala Pro Arg Leu
            500                 505                 510         
Ala Pro Glu Leu Tyr
        515         


<210> 5
<211> 316
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetically generated fragment

<400> 5
ctgcaacgtg atcctaaact ctggtctgat cctgatactt tcgatccaga gagattcatt 60
gctactgata ttgactttcg tggtcagtac tataagtata tcccgtttgg ttctggaaga 120
cgatcttgtc cagggatgac ttatgcattg caagtggaac acttaacaat ggcacatttg 180
atccaaggtt tcaattacag aactccaaat gacgagccct tggatatgaa ggaaggtgca 240
ggcataacta tacgtaaggt aaatcctgtg gaactgataa tagcgcctcg cctggcacct 300
gagctttatt aaaacc                                                 316

<210> 6
<211> 179
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetically generated fragment

<400> 6
ggtctgatcg tgaagatgat cgctggaaaa aattatgaat ccggtaaagg agatgaacaa 60
gtggagagat ttaagaaagc gtttaaggat tttatgattt tatcaatgga gtttgtgtta 120
tgggatgcat ttccaattcc attatttaaa tgggtggatt ttcaagggca tgttaaggc  179

<210> 7
<211> 254
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetically generated fragment

<400> 7
gggatgcatt tccaattcca ttatttaaat gggtggattt tcaagggcat gttaaggcta 60
tgaaaaggac ttttaaagat atagattctg tttttcagaa ttggttagag gaacatatta 120
ataaaagaga aaaaatggag gttaatgcag aagggaatga acaagatttc attgatgtgg 180
tgctttcaaa aatgagtaat gaatatcttg gtgaaggtta ctctcgtgat actgtcatta 240
aagcaacggt gttt                                                   254

<210> 8
<211> 120
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetically generated fragment

<400> 8
ttggatatga aggaaggtgc aggcataact atacgtaagg taaatcctgt ggaactgata 60
atagcgcctc gcctggcacc tgagctttat taaaacctaa gatctttcat cttggttgat 120


<210> 9
<211> 998
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetically generated fragment

<400> 9
gtaagttcat ctgtcatttt tcatttattc acttttattt tgaggagcag acatgttaat 60
aataatttgg agcaactgta aagttatcta tgtgtacagg ttcgagcctc aggtgcaacc 120
actaatgctt gtattagatt atgttgtctg catcataccc ctaattggag tgtggctctt 180
cccgaaccct gcaatgctgg atgctggatg ctttatgtat cagactgacc tttttgttaa 240
actatctaaa tactaaggat gatttaataa aaatatagaa tggtaaacag aaaaagatga 300
gattattttt ggggctatat ggattcgccc gggctttggg aggtaaaacg gtatctacca 360
gttgagactt tactccagaa ctttatctcg agagctctga ataaaaatga aatagtattt 420
accactccaa aatctttgat ggtaaaaaga tgagatataa cctcttataa ttgattgaac 480
cacgttgata gaataaaact tctttactcc cattcagcat aagaaaaatg aaaccaaacg 540
gaattcttct cttttttagg gggaaattcc ttaattgctt gttgaatata gattcatgtc 600
gttattctat ttttaataat gatgaaaatc aatatagtca aagttaatac ttatgtcatt 660
tggtttgcgg acaagttata ttggaactat ataatacgtc tattatagaa tagtgattat 720
ttagaggata tacatttttt ttggataaat atttgattta ttggattaaa aatagaatat 780
acaggtaagg tctaaaacgt gtgtttgctt ttacactaaa taaacttgac ctcgtacaat 840
tctaagaaaa tatttgaaat aaatgaatta ttttattgtt aatcaattaa aaaaatcata 900
gtatagatga gatgtgtgca tacttgacaa taactatact aactaaaaca aggtatgtga 960
ataattgata ttcctttttt aattattctt ttttccag                         998

<210> 10
<211> 431
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetically generated fragment

<400> 10
gtctgccagt tcagttcgtt gttcacacaa acggtgatac gtacactttt cccggcaata 60
acatacggcg tgacatcggc ttcaaatggc gtatagccgc cctgatgctc catcacttcc 120
tgattattga cccacacttt gccgtaatga gtgaccgcat cgaaacgcag cacgatacgc 180
tggcctgccc aacctttcgg tataaagact tcgcgctgat accagacgtt gcccgcataa 240
ttacgaatat ctgcatcggc gaactgatcg ttaaaactgc ctggcacagc aattgcccgg 300
ctttcttgta acgcgctttc ccaccaacgc tgatcaattc cacagttttc gcgatccaga 360
ctgaatgccc acaggccgtc gagttttttg atttcacggg ttggggtttc tacaggacgt 420
aaactagtca g                                                      431

<210> 11
<211> 155
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetically generated fragment

<400> 11
gtaataagat cttcaacacc tacaccattt ttttaatcac tactacccat tgcattgaac 60
aaacttccaa gttcttctta gcttcagatt aagaaagtac cctttcttgg ctttgttgat 120
gtggtaccat tgtccattgt cttgtgtgtt tccag                            155
