
                                SEQUENCE LISTING

<110> Dewey, Ralph E.
      Siminszky, Balazs
      Bowen, Steven W.
      Gavilano, Lily

<120> Alteration of Tobacco Alkaloid Content
  Through Modification of Specific Cytochrome P450 Genes
  

<130> 035051/333142

<150> 11/580,765
<151> 2006-10-13

<150> PCT/US2005/005665   
<151> 2005-02-23  

<160> 37

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 1733
<212> DNA
<213> Nicotiana sp.

<220> 
<221> CDS            
<222> (1)...(1551)

<400> 1
atg gtt ttt ccc ata gaa gcc ttt gta gga cta gta acc ttc aca ttt   48
Met Val Phe Pro Ile Glu Ala Phe Val Gly Leu Val Thr Phe Thr Phe
 1               5                   10                  15
 
ctc tta tac ttc cta tgg aca aaa aaa tct caa aaa ctt cca aaa ccc   96
Leu Leu Tyr Phe Leu Trp Thr Lys Lys Ser Gln Lys Leu Pro Lys Pro
             20                  25                  30
 
tta cca ccg aaa atc ccc gga gga tgg ccg gta atc ggc cat ctt ttt   144
Leu Pro Pro Lys Ile Pro Gly Gly Trp Pro Val Ile Gly His Leu Phe
         35                  40                  45
 
cac ttc aat aac gac ggc gac gac cgt cca tta gct cga aaa ctc gga   192
His Phe Asn Asn Asp Gly Asp Asp Arg Pro Leu Ala Arg Lys Leu Gly
     50                  55                  60
 
gac tta gct gat aaa tac ggc ccc gtt ttc act ttt cgg cta ggt ctt   240
Asp Leu Ala Asp Lys Tyr Gly Pro Val Phe Thr Phe Arg Leu Gly Leu
 65                  70                  75                  80
 
ccc ctt gtg cta gtt gta agc agt tac gaa gct ata aaa gat tgc ttc   288
Pro Leu Val Leu Val Val Ser Ser Tyr Glu Ala Ile Lys Asp Cys Phe
                 85                  90                  95
 
tct aca aat gac gcc att ttc tcc aat cgt cca gct ttt ctt tac ggc   336
Ser Thr Asn Asp Ala Ile Phe Ser Asn Arg Pro Ala Phe Leu Tyr Gly
            100                 105                 110
 
gaa tac ctt ggc tac aat aat aca atg ctt ttt cta gca aat tac gga   384
Glu Tyr Leu Gly Tyr Asn Asn Thr Met Leu Phe Leu Ala Asn Tyr Gly
        115                 120                 125
 
cct tac tgg cga aaa aat cgt aaa tta gtc att cag gaa gtt ctc tct   432
Pro Tyr Trp Arg Lys Asn Arg Lys Leu Val Ile Gln Glu Val Leu Ser
    130                 135                 140
 
gct agt cgt ctc gaa aaa ttc aaa caa gtg aga ttc acc aga att caa   480
Ala Ser Arg Leu Glu Lys Phe Lys Gln Val Arg Phe Thr Arg Ile Gln
145                 150                 155                 160
 
acg agc att aag aat tta tac act cga att aat gga aat tcg agt acg   528
Thr Ser Ile Lys Asn Leu Tyr Thr Arg Ile Asn Gly Asn Ser Ser Thr
                165                 170                 175
 
ata aat cta act gat tgg tta gaa gaa ttg aat ttt ggt ctg atc gtg   576
Ile Asn Leu Thr Asp Trp Leu Glu Glu Leu Asn Phe Gly Leu Ile Val
            180                 185                 190
 
aaa atg atc gct ggg aaa aat tat gaa tcc ggt aaa gga gat gaa caa   624
Lys Met Ile Ala Gly Lys Asn Tyr Glu Ser Gly Lys Gly Asp Glu Gln
        195                 200                 205
 
gtg gaa aga ttt aag aat gcg ttt aag gat ttt atg gtt tta tca atg   672
Val Glu Arg Phe Lys Asn Ala Phe Lys Asp Phe Met Val Leu Ser Met
    210                 215                 220
 
gaa ttt gta tta tgg gat gca ttt cca att cca tta ttt aaa tgg gtg   720
Glu Phe Val Leu Trp Asp Ala Phe Pro Ile Pro Leu Phe Lys Trp Val
225                 230                 235                 240
 
gat ttt caa ggt cat att aag gca atg aaa agg aca ttt aag gat ata   768
Asp Phe Gln Gly His Ile Lys Ala Met Lys Arg Thr Phe Lys Asp Ile
                245                 250                 255
 
gat tct gtt ttt cag aac tgg tta gag gaa cat att aat aaa aga gaa   816
Asp Ser Val Phe Gln Asn Trp Leu Glu Glu His Ile Asn Lys Arg Glu
            260                 265                 270
 
aaa atg gag gtt ggt gca gaa ggg aat gaa caa gat ttc att gat gtg   864
Lys Met Glu Val Gly Ala Glu Gly Asn Glu Gln Asp Phe Ile Asp Val
        275                 280                 285
 
gtg ctt tca aaa ttg agt aaa gaa tat ctt gat gaa ggt tac tct cgt   912
Val Leu Ser Lys Leu Ser Lys Glu Tyr Leu Asp Glu Gly Tyr Ser Arg
    290                 295                 300
 
gat act gtc att aaa gca aca gtt ttt agt ttg gtc ttg gat gca gca   960
Asp Thr Val Ile Lys Ala Thr Val Phe Ser Leu Val Leu Asp Ala Ala
305                 310                 315                 320
 
gac aca gtt gct ctt cac ata aat tgg gga atg aca tta ttg ata aac   1008
Asp Thr Val Ala Leu His Ile Asn Trp Gly Met Thr Leu Leu Ile Asn
                325                 330                 335
 
aat caa aat gcc ttg atg aaa gca caa gaa gag ata gac aca aaa gtt   1056
Asn Gln Asn Ala Leu Met Lys Ala Gln Glu Glu Ile Asp Thr Lys Val
            340                 345                 350
 
ggt aag gat aga tgg gta gaa gag agt gat att aag gat tta gta tac   1104
Gly Lys Asp Arg Trp Val Glu Glu Ser Asp Ile Lys Asp Leu Val Tyr
        355                 360                 365
 
ctc caa gct att gtt aaa aag gtg tta cga tta tat cca cca gga cct   1152
Leu Gln Ala Ile Val Lys Lys Val Leu Arg Leu Tyr Pro Pro Gly Pro
    370                 375                 380
 
ttg tta gta cca cat gaa aat gta aag gat tgt gtt gtt agt gga tat   1200
Leu Leu Val Pro His Glu Asn Val Lys Asp Cys Val Val Ser Gly Tyr
385                 390                 395                 400
 
cac att cct aaa gag act aga tta ttc gca aac gtc atg aaa ctg cag   1248
His Ile Pro Lys Glu Thr Arg Leu Phe Ala Asn Val Met Lys Leu Gln
                405                 410                 415
 
cgc gat cct aaa ctc ttg tca aat cct gat aag ttc gat cca gag aga   1296
Arg Asp Pro Lys Leu Leu Ser Asn Pro Asp Lys Phe Asp Pro Glu Arg
            420                 425                 430
 
ttc atc gct ggt gat att gac ttc cgt ggt cac cac tat gag ttt atc   1344
Phe Ile Ala Gly Asp Ile Asp Phe Arg Gly His His Tyr Glu Phe Ile
        435                 440                 445
 
cca ttt ggt tct gga aga cga tct tgt ccg ggg atg act tat gca ttg   1392
Pro Phe Gly Ser Gly Arg Arg Ser Cys Pro Gly Met Thr Tyr Ala Leu
    450                 455                 460
 
caa gtg gaa cac cta aca atg gca cat tta atc cag ggt ttc aat tac   1440
Gln Val Glu His Leu Thr Met Ala His Leu Ile Gln Gly Phe Asn Tyr
465                 470                 475                 480
 
aaa act cca aat gac gag gcc ttg gat atg aag gaa ggt gca ggc ata   1488
Lys Thr Pro Asn Asp Glu Ala Leu Asp Met Lys Glu Gly Ala Gly Ile
                485                 490                 495
 
acc ata cgt aag gta aat cca gtg gaa ttg ata ata acg cct cgc ttg   1536
Thr Ile Arg Lys Val Asn Pro Val Glu Leu Ile Ile Thr Pro Arg Leu
            500                 505                 510
 
gca cct gag ctt tac taaaacctaa gatctttcat cttggttgat cattgtttaa   1591
Ala Pro Glu Leu Tyr
        515
 
tactcctaga tgggtattca tttacctttt ttcaattaat tgcatgtacg agctttttta 1651
atttggtata tttgtaacaa taagtaaaga atgattgtgc taatatataa agatttgcag 1711
aagataattg actgattgtc cc                                          1733

<210> 2
<211> 517
<212> PRT
<213> Nicotiana sp.

<400> 2
Met Val Phe Pro Ile Glu Ala Phe Val Gly Leu Val Thr Phe Thr Phe
 1               5                  10                  15      
Leu Leu Tyr Phe Leu Trp Thr Lys Lys Ser Gln Lys Leu Pro Lys Pro
            20                  25                  30          
Leu Pro Pro Lys Ile Pro Gly Gly Trp Pro Val Ile Gly His Leu Phe
        35                  40                  45              
His Phe Asn Asn Asp Gly Asp Asp Arg Pro Leu Ala Arg Lys Leu Gly
    50                  55                  60                  
Asp Leu Ala Asp Lys Tyr Gly Pro Val Phe Thr Phe Arg Leu Gly Leu
65                  70                  75                  80  
Pro Leu Val Leu Val Val Ser Ser Tyr Glu Ala Ile Lys Asp Cys Phe
                85                  90                  95      
Ser Thr Asn Asp Ala Ile Phe Ser Asn Arg Pro Ala Phe Leu Tyr Gly
            100                 105                 110         
Glu Tyr Leu Gly Tyr Asn Asn Thr Met Leu Phe Leu Ala Asn Tyr Gly
        115                 120                 125             
Pro Tyr Trp Arg Lys Asn Arg Lys Leu Val Ile Gln Glu Val Leu Ser
    130                 135                 140                 
Ala Ser Arg Leu Glu Lys Phe Lys Gln Val Arg Phe Thr Arg Ile Gln
145                 150                 155                 160 
Thr Ser Ile Lys Asn Leu Tyr Thr Arg Ile Asn Gly Asn Ser Ser Thr
                165                 170                 175     
Ile Asn Leu Thr Asp Trp Leu Glu Glu Leu Asn Phe Gly Leu Ile Val
            180                 185                 190         
Lys Met Ile Ala Gly Lys Asn Tyr Glu Ser Gly Lys Gly Asp Glu Gln
        195                 200                 205             
Val Glu Arg Phe Lys Asn Ala Phe Lys Asp Phe Met Val Leu Ser Met
    210                 215                 220                 
Glu Phe Val Leu Trp Asp Ala Phe Pro Ile Pro Leu Phe Lys Trp Val
225                 230                 235                 240 
Asp Phe Gln Gly His Ile Lys Ala Met Lys Arg Thr Phe Lys Asp Ile
                245                 250                 255     
Asp Ser Val Phe Gln Asn Trp Leu Glu Glu His Ile Asn Lys Arg Glu
            260                 265                 270         
Lys Met Glu Val Gly Ala Glu Gly Asn Glu Gln Asp Phe Ile Asp Val
        275                 280                 285             
Val Leu Ser Lys Leu Ser Lys Glu Tyr Leu Asp Glu Gly Tyr Ser Arg
    290                 295                 300                 
Asp Thr Val Ile Lys Ala Thr Val Phe Ser Leu Val Leu Asp Ala Ala
305                 310                 315                 320 
Asp Thr Val Ala Leu His Ile Asn Trp Gly Met Thr Leu Leu Ile Asn
                325                 330                 335     
Asn Gln Asn Ala Leu Met Lys Ala Gln Glu Glu Ile Asp Thr Lys Val
            340                 345                 350         
Gly Lys Asp Arg Trp Val Glu Glu Ser Asp Ile Lys Asp Leu Val Tyr
        355                 360                 365             
Leu Gln Ala Ile Val Lys Lys Val Leu Arg Leu Tyr Pro Pro Gly Pro
    370                 375                 380                 
Leu Leu Val Pro His Glu Asn Val Lys Asp Cys Val Val Ser Gly Tyr
385                 390                 395                 400 
His Ile Pro Lys Glu Thr Arg Leu Phe Ala Asn Val Met Lys Leu Gln
                405                 410                 415     
Arg Asp Pro Lys Leu Leu Ser Asn Pro Asp Lys Phe Asp Pro Glu Arg
            420                 425                 430         
Phe Ile Ala Gly Asp Ile Asp Phe Arg Gly His His Tyr Glu Phe Ile
        435                 440                 445             
Pro Phe Gly Ser Gly Arg Arg Ser Cys Pro Gly Met Thr Tyr Ala Leu
    450                 455                 460                 
Gln Val Glu His Leu Thr Met Ala His Leu Ile Gln Gly Phe Asn Tyr
465                 470                 475                 480 
Lys Thr Pro Asn Asp Glu Ala Leu Asp Met Lys Glu Gly Ala Gly Ile
                485                 490                 495     
Thr Ile Arg Lys Val Asn Pro Val Glu Leu Ile Ile Thr Pro Arg Leu
            500                 505                 510         
Ala Pro Glu Leu Tyr
        515         


<210> 3
<211> 1733
<212> DNA
<213> Nicotiana sp.

<220> 
<221> CDS            
<222> (1)...(1551)

<400> 3
atg ctt tct ccc ata gaa gcc att gta gga cta gta acc ttc aca ttt   48
Met Leu Ser Pro Ile Glu Ala Ile Val Gly Leu Val Thr Phe Thr Phe
 1               5                   10                  15
 
ctc ttc ttc ttc cta tgg aca aaa aaa tct caa aaa cct tca aaa ccc   96
Leu Phe Phe Phe Leu Trp Thr Lys Lys Ser Gln Lys Pro Ser Lys Pro
             20                  25                  30
 
tta cca ccg aaa atc ccc gga gga tgg ccg gta atc ggc cat ctt ttc   144
Leu Pro Pro Lys Ile Pro Gly Gly Trp Pro Val Ile Gly His Leu Phe
         35                  40                  45
 
cac ttc aat gac gac ggc gac gac cgt cca tta gct cga aaa ctc gga   192
His Phe Asn Asp Asp Gly Asp Asp Arg Pro Leu Ala Arg Lys Leu Gly
     50                  55                  60
 
gac tta gct gac aaa tac ggc ccc gtt ttc act ttt cgg cta ggc ctt   240
Asp Leu Ala Asp Lys Tyr Gly Pro Val Phe Thr Phe Arg Leu Gly Leu
 65                  70                  75                  80
 
ccc ctt gtc tta gtt gta agc agt tac gaa gct gta aaa gac tgt ttc   288
Pro Leu Val Leu Val Val Ser Ser Tyr Glu Ala Val Lys Asp Cys Phe
                 85                  90                  95
 
tct aca aat gac gcc att ttt tcc aat cgt cca gct ttt ctt tac ggc   336
Ser Thr Asn Asp Ala Ile Phe Ser Asn Arg Pro Ala Phe Leu Tyr Gly
            100                 105                 110
 
gat tac ctt ggc tac aat aat gcc atg cta ttt ttg gcc aat tac gga   384
Asp Tyr Leu Gly Tyr Asn Asn Ala Met Leu Phe Leu Ala Asn Tyr Gly
        115                 120                 125
 
cct tac tgg cga aaa aat cga aaa tta gtt att cag gaa gtt ctc tcc   432
Pro Tyr Trp Arg Lys Asn Arg Lys Leu Val Ile Gln Glu Val Leu Ser
    130                 135                 140
 
gct agt cgt ctc gaa aaa ttc aaa cac gtg aga ttt gca aga att caa   480
Ala Ser Arg Leu Glu Lys Phe Lys His Val Arg Phe Ala Arg Ile Gln
145                 150                 155                 160
 
gcg agc att aag aat tta tat act cga att gat gga aat tcg agt acg   528
Ala Ser Ile Lys Asn Leu Tyr Thr Arg Ile Asp Gly Asn Ser Ser Thr
                165                 170                 175
 
ata aat tta act gat tgg tta gaa gaa ttg aat ttt ggt ctg atc gtg   576
Ile Asn Leu Thr Asp Trp Leu Glu Glu Leu Asn Phe Gly Leu Ile Val
            180                 185                 190
 
aag atg atc gct gga aaa aat tat gaa tcc ggt aaa gga gat gaa caa   624
Lys Met Ile Ala Gly Lys Asn Tyr Glu Ser Gly Lys Gly Asp Glu Gln
        195                 200                 205
 
gtg gag aga ttt aag aaa gcg ttt aag gat ttt atg att tta tca atg   672
Val Glu Arg Phe Lys Lys Ala Phe Lys Asp Phe Met Ile Leu Ser Met
    210                 215                 220
 
gag ttt gtg tta tgg gat gca ttt cca att cca tta ttt aaa tgg gtg   720
Glu Phe Val Leu Trp Asp Ala Phe Pro Ile Pro Leu Phe Lys Trp Val
225                 230                 235                 240
 
gat ttt caa ggg cat gtt aag gct atg aaa agg act ttt aaa gat ata   768
Asp Phe Gln Gly His Val Lys Ala Met Lys Arg Thr Phe Lys Asp Ile
                245                 250                 255
 
gat tct gtt ttt cag aat tgg tta gag gaa cat att aat aaa aga gaa   816
Asp Ser Val Phe Gln Asn Trp Leu Glu Glu His Ile Asn Lys Arg Glu
            260                 265                 270
 
aaa atg gag gtt aat gca gaa ggg aat gaa caa gat ttc att gat gtg   864
Lys Met Glu Val Asn Ala Glu Gly Asn Glu Gln Asp Phe Ile Asp Val
        275                 280                 285
 
gtg ctt tca aaa atg agt aat gaa tat ctt ggt gaa ggt tac tct cgt   912
Val Leu Ser Lys Met Ser Asn Glu Tyr Leu Gly Glu Gly Tyr Ser Arg
    290                 295                 300
 
gat act gtc att aaa gca acg gtg ttt agt ttg gtc ttg gat gca gca   960
Asp Thr Val Ile Lys Ala Thr Val Phe Ser Leu Val Leu Asp Ala Ala
305                 310                 315                 320
 
gac aca gtt gct ctt cac ata aat tgg gga atg gca tta ttg ata aac   1008
Asp Thr Val Ala Leu His Ile Asn Trp Gly Met Ala Leu Leu Ile Asn
                325                 330                 335
 
aat caa aag gcc ttg acg aaa gca caa gaa gag ata gac aca aaa gtt   1056
Asn Gln Lys Ala Leu Thr Lys Ala Gln Glu Glu Ile Asp Thr Lys Val
            340                 345                 350
 
ggt aag gac aga tgg gta gaa gag agt gat att aag gat ttg gta tac   1104
Gly Lys Asp Arg Trp Val Glu Glu Ser Asp Ile Lys Asp Leu Val Tyr
        355                 360                 365
 
ctc caa gct att gtt aaa gaa gtg tta cga tta tat cca cca gga cct   1152
Leu Gln Ala Ile Val Lys Glu Val Leu Arg Leu Tyr Pro Pro Gly Pro
    370                 375                 380
 
ttg tta gta cca cac gaa aat gta gaa gat tgt gtt gtt agt gga tat   1200
Leu Leu Val Pro His Glu Asn Val Glu Asp Cys Val Val Ser Gly Tyr
385                 390                 395                 400
 
cac att cct aaa ggg aca aga tta ttc gca aac gtc atg aaa ctg caa   1248
His Ile Pro Lys Gly Thr Arg Leu Phe Ala Asn Val Met Lys Leu Gln
                405                 410                 415
 
cgt gat cct aaa ctc tgg tct gat cct gat act ttc gat cca gag aga   1296
Arg Asp Pro Lys Leu Trp Ser Asp Pro Asp Thr Phe Asp Pro Glu Arg
            420                 425                 430
 
ttc att gct act gat att gac ttt cgt ggt cag tac tat aag tat atc   1344
Phe Ile Ala Thr Asp Ile Asp Phe Arg Gly Gln Tyr Tyr Lys Tyr Ile
        435                 440                 445
 
ccg ttt ggt tct gga aga cga tct tgt cca ggg atg act tat gca ttg   1392
Pro Phe Gly Ser Gly Arg Arg Ser Cys Pro Gly Met Thr Tyr Ala Leu
    450                 455                 460
 
caa gtg gaa cac tta aca atg gca cat ttg atc caa ggt ttc aat tac   1440
Gln Val Glu His Leu Thr Met Ala His Leu Ile Gln Gly Phe Asn Tyr
465                 470                 475                 480
 
aga act cca aat gac gag ccc ttg gat atg aag gaa ggt gca ggc ata   1488
Arg Thr Pro Asn Asp Glu Pro Leu Asp Met Lys Glu Gly Ala Gly Ile
                485                 490                 495
 
act ata cgt aag gta aat cct gtg gaa ctg ata ata gcg cct cgc ctg   1536
Thr Ile Arg Lys Val Asn Pro Val Glu Leu Ile Ile Ala Pro Arg Leu
            500                 505                 510
 
gca cct gag ctt tat taaaacctaa gatctttcat cttggttgat cattgtataa   1591
Ala Pro Glu Leu Tyr
        515
 
tactcctaaa tggatattca tttacctttt atcaattaat tgtcagtacg agtttttcta 1651
atttggtaca tttgtaataa taagtaaaga ataattgtgc taatatataa aggtttgtag 1711
aagataattg actgattgtc cc                                          1733

<210> 4
<211> 517
<212> PRT
<213> Nicotiana sp.

<400> 4
Met Leu Ser Pro Ile Glu Ala Ile Val Gly Leu Val Thr Phe Thr Phe
 1               5                  10                  15      
Leu Phe Phe Phe Leu Trp Thr Lys Lys Ser Gln Lys Pro Ser Lys Pro
            20                  25                  30          
Leu Pro Pro Lys Ile Pro Gly Gly Trp Pro Val Ile Gly His Leu Phe
        35                  40                  45              
His Phe Asn Asp Asp Gly Asp Asp Arg Pro Leu Ala Arg Lys Leu Gly
    50                  55                  60                  
Asp Leu Ala Asp Lys Tyr Gly Pro Val Phe Thr Phe Arg Leu Gly Leu
65                  70                  75                  80  
Pro Leu Val Leu Val Val Ser Ser Tyr Glu Ala Val Lys Asp Cys Phe
                85                  90                  95      
Ser Thr Asn Asp Ala Ile Phe Ser Asn Arg Pro Ala Phe Leu Tyr Gly
            100                 105                 110         
Asp Tyr Leu Gly Tyr Asn Asn Ala Met Leu Phe Leu Ala Asn Tyr Gly
        115                 120                 125             
Pro Tyr Trp Arg Lys Asn Arg Lys Leu Val Ile Gln Glu Val Leu Ser
    130                 135                 140                 
Ala Ser Arg Leu Glu Lys Phe Lys His Val Arg Phe Ala Arg Ile Gln
145                 150                 155                 160 
Ala Ser Ile Lys Asn Leu Tyr Thr Arg Ile Asp Gly Asn Ser Ser Thr
                165                 170                 175     
Ile Asn Leu Thr Asp Trp Leu Glu Glu Leu Asn Phe Gly Leu Ile Val
            180                 185                 190         
Lys Met Ile Ala Gly Lys Asn Tyr Glu Ser Gly Lys Gly Asp Glu Gln
        195                 200                 205             
Val Glu Arg Phe Lys Lys Ala Phe Lys Asp Phe Met Ile Leu Ser Met
    210                 215                 220                 
Glu Phe Val Leu Trp Asp Ala Phe Pro Ile Pro Leu Phe Lys Trp Val
225                 230                 235                 240 
Asp Phe Gln Gly His Val Lys Ala Met Lys Arg Thr Phe Lys Asp Ile
                245                 250                 255     
Asp Ser Val Phe Gln Asn Trp Leu Glu Glu His Ile Asn Lys Arg Glu
            260                 265                 270         
Lys Met Glu Val Asn Ala Glu Gly Asn Glu Gln Asp Phe Ile Asp Val
        275                 280                 285             
Val Leu Ser Lys Met Ser Asn Glu Tyr Leu Gly Glu Gly Tyr Ser Arg
    290                 295                 300                 
Asp Thr Val Ile Lys Ala Thr Val Phe Ser Leu Val Leu Asp Ala Ala
305                 310                 315                 320 
Asp Thr Val Ala Leu His Ile Asn Trp Gly Met Ala Leu Leu Ile Asn
                325                 330                 335     
Asn Gln Lys Ala Leu Thr Lys Ala Gln Glu Glu Ile Asp Thr Lys Val
            340                 345                 350         
Gly Lys Asp Arg Trp Val Glu Glu Ser Asp Ile Lys Asp Leu Val Tyr
        355                 360                 365             
Leu Gln Ala Ile Val Lys Glu Val Leu Arg Leu Tyr Pro Pro Gly Pro
    370                 375                 380                 
Leu Leu Val Pro His Glu Asn Val Glu Asp Cys Val Val Ser Gly Tyr
385                 390                 395                 400 
His Ile Pro Lys Gly Thr Arg Leu Phe Ala Asn Val Met Lys Leu Gln
                405                 410                 415     
Arg Asp Pro Lys Leu Trp Ser Asp Pro Asp Thr Phe Asp Pro Glu Arg
            420                 425                 430         
Phe Ile Ala Thr Asp Ile Asp Phe Arg Gly Gln Tyr Tyr Lys Tyr Ile
        435                 440                 445             
Pro Phe Gly Ser Gly Arg Arg Ser Cys Pro Gly Met Thr Tyr Ala Leu
    450                 455                 460                 
Gln Val Glu His Leu Thr Met Ala His Leu Ile Gln Gly Phe Asn Tyr
465                 470                 475                 480 
Arg Thr Pro Asn Asp Glu Pro Leu Asp Met Lys Glu Gly Ala Gly Ile
                485                 490                 495     
Thr Ile Arg Lys Val Asn Pro Val Glu Leu Ile Ile Ala Pro Arg Leu
            500                 505                 510         
Ala Pro Glu Leu Tyr
        515         


<210> 5
<211> 1733
<212> DNA
<213> Nicotiana sp.

<220> 
<221> CDS            
<222> (1)...(1551)

<400> 5
atg gtt ttt ccc ata gaa gcc att gta gga cta gta acc ttc aca ttt   48
Met Val Phe Pro Ile Glu Ala Ile Val Gly Leu Val Thr Phe Thr Phe
 1               5                   10                  15
 
ctc ttc ttc ttc cta tgg aca aaa aaa tct caa aaa cct tca aaa ccc   96
Leu Phe Phe Phe Leu Trp Thr Lys Lys Ser Gln Lys Pro Ser Lys Pro
             20                  25                  30
 
tta cca ccg aaa atc ccc gga gga tgg ccg gta atc ggc cat ctt ttc   144
Leu Pro Pro Lys Ile Pro Gly Gly Trp Pro Val Ile Gly His Leu Phe
         35                  40                  45
 
cac ttc aat gac gac ggc gac gac cgt cca tta gct cga aaa ctc gga   192
His Phe Asn Asp Asp Gly Asp Asp Arg Pro Leu Ala Arg Lys Leu Gly
     50                  55                  60
 
gac tta gct gac aaa tac ggc ccc gtt ttc act ttt cgg cta ggc ctt   240
Asp Leu Ala Asp Lys Tyr Gly Pro Val Phe Thr Phe Arg Leu Gly Leu
 65                  70                  75                  80
 
ccc ctt gtc tta gtt gta agc agt tac gaa gct gta aaa gac tgt ttc   288
Pro Leu Val Leu Val Val Ser Ser Tyr Glu Ala Val Lys Asp Cys Phe
                 85                  90                  95
 
tct aca aat gac gcc att ttt tcc aat cgt cca gct ttt ctt tac ggc   336
Ser Thr Asn Asp Ala Ile Phe Ser Asn Arg Pro Ala Phe Leu Tyr Gly
            100                 105                 110
 
gat tac ctt ggc tac aat aat gcc atg cta ttt ttg gcc aat tac gga   384
Asp Tyr Leu Gly Tyr Asn Asn Ala Met Leu Phe Leu Ala Asn Tyr Gly
        115                 120                 125
 
cct tac tgg cga aaa aat cga aaa tta gtt att cag gaa gtt ctc tcc   432
Pro Tyr Trp Arg Lys Asn Arg Lys Leu Val Ile Gln Glu Val Leu Ser
    130                 135                 140
 
gct agt cgt ctc gaa aaa ttc aaa cac gtg aga ttt gca aga att caa   480
Ala Ser Arg Leu Glu Lys Phe Lys His Val Arg Phe Ala Arg Ile Gln
145                 150                 155                 160
 
gcg agc att aag aat tta tat act cga att gat gga aat tcg agt acg   528
Ala Ser Ile Lys Asn Leu Tyr Thr Arg Ile Asp Gly Asn Ser Ser Thr
                165                 170                 175
 
ata aat tta act gat tgg tta gaa gaa ttg aat ttt ggt ctg atc gtg   576
Ile Asn Leu Thr Asp Trp Leu Glu Glu Leu Asn Phe Gly Leu Ile Val
            180                 185                 190
 
aag atg atc gct gga aaa aat tat gaa tcc ggt aaa gga gat gaa caa   624
Lys Met Ile Ala Gly Lys Asn Tyr Glu Ser Gly Lys Gly Asp Glu Gln
        195                 200                 205
 
gtg gag aga ttt aag aaa gcg ttt aag gat ttt atg att tta tca atg   672
Val Glu Arg Phe Lys Lys Ala Phe Lys Asp Phe Met Ile Leu Ser Met
    210                 215                 220
 
gag ttt gtg tta tgg gat gca ttt cca att cca tta ttt aaa tgg gtg   720
Glu Phe Val Leu Trp Asp Ala Phe Pro Ile Pro Leu Phe Lys Trp Val
225                 230                 235                 240
 
gat ttt caa ggg cat gtt aag gct atg aaa agg act ttt aaa gat ata   768
Asp Phe Gln Gly His Val Lys Ala Met Lys Arg Thr Phe Lys Asp Ile
                245                 250                 255
 
gat tct gtt ttt cag aat tgg tta gag gaa cat att aat aaa aga gaa   816
Asp Ser Val Phe Gln Asn Trp Leu Glu Glu His Ile Asn Lys Arg Glu
            260                 265                 270
 
aaa atg gag gtt aat gca gaa ggg aat gaa caa gat ttc att gat gtg   864
Lys Met Glu Val Asn Ala Glu Gly Asn Glu Gln Asp Phe Ile Asp Val
        275                 280                 285
 
gtg ctt tca aaa atg agt aat gaa tat ctt ggt gaa ggt tac tct cgt   912
Val Leu Ser Lys Met Ser Asn Glu Tyr Leu Gly Glu Gly Tyr Ser Arg
    290                 295                 300
 
gat act gtc att aag gca acg gtg ttt agt ttg gtc ttg gat gca gca   960
Asp Thr Val Ile Lys Ala Thr Val Phe Ser Leu Val Leu Asp Ala Ala
305                 310                 315                 320
 
gac aca gtt gct ctt cac ata aat tgg gga atg gca tta ttg ata aac   1008
Asp Thr Val Ala Leu His Ile Asn Trp Gly Met Ala Leu Leu Ile Asn
                325                 330                 335
 
aat caa aag gcc ttg acg aaa gca caa gaa gag ata gac aca aaa gtt   1056
Asn Gln Lys Ala Leu Thr Lys Ala Gln Glu Glu Ile Asp Thr Lys Val
            340                 345                 350
 
ggt aag gac aga tgg gta gaa gag agt gat att aag gat ttg gta tac   1104
Gly Lys Asp Arg Trp Val Glu Glu Ser Asp Ile Lys Asp Leu Val Tyr
        355                 360                 365
 
ctc caa gct att gtt aaa gaa gtg tta cga tta tat cca cca gga cct   1152
Leu Gln Ala Ile Val Lys Glu Val Leu Arg Leu Tyr Pro Pro Gly Pro
    370                 375                 380
 
ttg tta gta cca cac gaa aat gta gaa gat tgt gtt gtt agt gga tat   1200
Leu Leu Val Pro His Glu Asn Val Glu Asp Cys Val Val Ser Gly Tyr
385                 390                 395                 400
 
cac att cct aaa ggg aca aga tta ttc gca aac gtc atg aaa ctg caa   1248
His Ile Pro Lys Gly Thr Arg Leu Phe Ala Asn Val Met Lys Leu Gln
                405                 410                 415
 
cgt gat cct aaa ctc tgg tct gat cct gat act ttc gat cca gag aga   1296
Arg Asp Pro Lys Leu Trp Ser Asp Pro Asp Thr Phe Asp Pro Glu Arg
            420                 425                 430
 
ttc att gct act gat att gac ttt cgt ggt cag tac tat aag tat atc   1344
Phe Ile Ala Thr Asp Ile Asp Phe Arg Gly Gln Tyr Tyr Lys Tyr Ile
        435                 440                 445
 
ccg ttt ggt tct gga aga cga tct tgt cca ggg atg act tat gca ttg   1392
Pro Phe Gly Ser Gly Arg Arg Ser Cys Pro Gly Met Thr Tyr Ala Leu
    450                 455                 460
 
caa gtg gaa cac tta aca atg gca cat ttg atc caa ggt ttc aat tac   1440
Gln Val Glu His Leu Thr Met Ala His Leu Ile Gln Gly Phe Asn Tyr
465                 470                 475                 480
 
aga act cca aat gac gag ccc ttg gac atg aag gaa ggt gca ggc ata   1488
Arg Thr Pro Asn Asp Glu Pro Leu Asp Met Lys Glu Gly Ala Gly Ile
                485                 490                 495
 
act ata cgt aag gta aat cct gtg gaa ctg ata ata gcg cct cgc ctg   1536
Thr Ile Arg Lys Val Asn Pro Val Glu Leu Ile Ile Ala Pro Arg Leu
            500                 505                 510
 
gca cct gag ctt tat taaaacctaa gatctttcat cttggttgat cattgtataa   1591
Ala Pro Glu Leu Tyr
        515
 
tactcctaaa tggatattca tttacctttt atcaattaat tgtcagtacg agtttttcta 1651
atttggtaca tttgtaataa taagtaaaga ataattgtgc taatatataa aggtttgtag 1711
aagatgattg actgattgtc cc                                          1733

<210> 6
<211> 517
<212> PRT
<213> Nicotiana sp.

<400> 6
Met Val Phe Pro Ile Glu Ala Ile Val Gly Leu Val Thr Phe Thr Phe
 1               5                  10                  15      
Leu Phe Phe Phe Leu Trp Thr Lys Lys Ser Gln Lys Pro Ser Lys Pro
            20                  25                  30          
Leu Pro Pro Lys Ile Pro Gly Gly Trp Pro Val Ile Gly His Leu Phe
        35                  40                  45              
His Phe Asn Asp Asp Gly Asp Asp Arg Pro Leu Ala Arg Lys Leu Gly
    50                  55                  60                  
Asp Leu Ala Asp Lys Tyr Gly Pro Val Phe Thr Phe Arg Leu Gly Leu
65                  70                  75                  80  
Pro Leu Val Leu Val Val Ser Ser Tyr Glu Ala Val Lys Asp Cys Phe
                85                  90                  95      
Ser Thr Asn Asp Ala Ile Phe Ser Asn Arg Pro Ala Phe Leu Tyr Gly
            100                 105                 110         
Asp Tyr Leu Gly Tyr Asn Asn Ala Met Leu Phe Leu Ala Asn Tyr Gly
        115                 120                 125             
Pro Tyr Trp Arg Lys Asn Arg Lys Leu Val Ile Gln Glu Val Leu Ser
    130                 135                 140                 
Ala Ser Arg Leu Glu Lys Phe Lys His Val Arg Phe Ala Arg Ile Gln
145                 150                 155                 160 
Ala Ser Ile Lys Asn Leu Tyr Thr Arg Ile Asp Gly Asn Ser Ser Thr
                165                 170                 175     
Ile Asn Leu Thr Asp Trp Leu Glu Glu Leu Asn Phe Gly Leu Ile Val
            180                 185                 190         
Lys Met Ile Ala Gly Lys Asn Tyr Glu Ser Gly Lys Gly Asp Glu Gln
        195                 200                 205             
Val Glu Arg Phe Lys Lys Ala Phe Lys Asp Phe Met Ile Leu Ser Met
    210                 215                 220                 
Glu Phe Val Leu Trp Asp Ala Phe Pro Ile Pro Leu Phe Lys Trp Val
225                 230                 235                 240 
Asp Phe Gln Gly His Val Lys Ala Met Lys Arg Thr Phe Lys Asp Ile
                245                 250                 255     
Asp Ser Val Phe Gln Asn Trp Leu Glu Glu His Ile Asn Lys Arg Glu
            260                 265                 270         
Lys Met Glu Val Asn Ala Glu Gly Asn Glu Gln Asp Phe Ile Asp Val
        275                 280                 285             
Val Leu Ser Lys Met Ser Asn Glu Tyr Leu Gly Glu Gly Tyr Ser Arg
    290                 295                 300                 
Asp Thr Val Ile Lys Ala Thr Val Phe Ser Leu Val Leu Asp Ala Ala
305                 310                 315                 320 
Asp Thr Val Ala Leu His Ile Asn Trp Gly Met Ala Leu Leu Ile Asn
                325                 330                 335     
Asn Gln Lys Ala Leu Thr Lys Ala Gln Glu Glu Ile Asp Thr Lys Val
            340                 345                 350         
Gly Lys Asp Arg Trp Val Glu Glu Ser Asp Ile Lys Asp Leu Val Tyr
        355                 360                 365             
Leu Gln Ala Ile Val Lys Glu Val Leu Arg Leu Tyr Pro Pro Gly Pro
    370                 375                 380                 
Leu Leu Val Pro His Glu Asn Val Glu Asp Cys Val Val Ser Gly Tyr
385                 390                 395                 400 
His Ile Pro Lys Gly Thr Arg Leu Phe Ala Asn Val Met Lys Leu Gln
                405                 410                 415     
Arg Asp Pro Lys Leu Trp Ser Asp Pro Asp Thr Phe Asp Pro Glu Arg
            420                 425                 430         
Phe Ile Ala Thr Asp Ile Asp Phe Arg Gly Gln Tyr Tyr Lys Tyr Ile
        435                 440                 445             
Pro Phe Gly Ser Gly Arg Arg Ser Cys Pro Gly Met Thr Tyr Ala Leu
    450                 455                 460                 
Gln Val Glu His Leu Thr Met Ala His Leu Ile Gln Gly Phe Asn Tyr
465                 470                 475                 480 
Arg Thr Pro Asn Asp Glu Pro Leu Asp Met Lys Glu Gly Ala Gly Ile
                485                 490                 495     
Thr Ile Arg Lys Val Asn Pro Val Glu Leu Ile Ile Ala Pro Arg Leu
            500                 505                 510         
Ala Pro Glu Leu Tyr
        515         


<210> 7
<211> 1727
<212> DNA
<213> Nicotiana sp.

<220> 
<221> CDS            
<222> (1)...(1554)

<400> 7
atg gtt ttt ccg gta gaa gcc att gta ggg cta gtg acc ttc aca ttt   48
Met Val Phe Pro Val Glu Ala Ile Val Gly Leu Val Thr Phe Thr Phe
 1               5                   10                  15
 
ctc ttc tac ttc cta tgg aca aaa aaa tct caa aaa cct tca aaa ccc   96
Leu Phe Tyr Phe Leu Trp Thr Lys Lys Ser Gln Lys Pro Ser Lys Pro
             20                  25                  30
 
tta cca ccg aaa atc ccg gga gga tgg ccg gta atc ggc cat ctt ttc   144
Leu Pro Pro Lys Ile Pro Gly Gly Trp Pro Val Ile Gly His Leu Phe
         35                  40                  45
 
tac ttc gat gac gac ggc gac gac cgt cca tta gct cga aaa ctc gga   192
Tyr Phe Asp Asp Asp Gly Asp Asp Arg Pro Leu Ala Arg Lys Leu Gly
     50                  55                  60
 
gac tta gct gac aaa tac gga ccc gtt ttc act ttt cgg cta ggc ctt   240
Asp Leu Ala Asp Lys Tyr Gly Pro Val Phe Thr Phe Arg Leu Gly Leu
 65                  70                  75                  80
 
ccc ctt gtg tta gtt gta agc agt tac gaa gct ata aaa gat tgt ttc   288
Pro Leu Val Leu Val Val Ser Ser Tyr Glu Ala Ile Lys Asp Cys Phe
                 85                  90                  95
 
tct aca aat gac gcc att ttc tcc aat cgt cca gct ttt ctt tac ggc   336
Ser Thr Asn Asp Ala Ile Phe Ser Asn Arg Pro Ala Phe Leu Tyr Gly
            100                 105                 110
 
gaa tac ctt ggc tac aaa aat gcc atg cta ttt ttg gca aat tac gga   384
Glu Tyr Leu Gly Tyr Lys Asn Ala Met Leu Phe Leu Ala Asn Tyr Gly
        115                 120                 125
 
tct tac tgg cga aaa aat cgt aaa tta att att cag gaa gtt ctc tct   432
Ser Tyr Trp Arg Lys Asn Arg Lys Leu Ile Ile Gln Glu Val Leu Ser
    130                 135                 140
 
gct agt cgt ctc gaa aaa ttc aaa cac gtg aga ttc gcc aga att caa   480
Ala Ser Arg Leu Glu Lys Phe Lys His Val Arg Phe Ala Arg Ile Gln
145                 150                 155                 160
 
acg agc att aag aat tta tac act cga att gat gga aat tcg agt acg   528
Thr Ser Ile Lys Asn Leu Tyr Thr Arg Ile Asp Gly Asn Ser Ser Thr
                165                 170                 175
 
ata aat tta act gat tgg tta gaa gaa ttg aat ttt ggt ctg atc gtg   576
Ile Asn Leu Thr Asp Trp Leu Glu Glu Leu Asn Phe Gly Leu Ile Val
            180                 185                 190
 
aaa atg atc gct ggg aaa aat tat gaa tcc ggt aaa gga gat gaa caa   624
Lys Met Ile Ala Gly Lys Asn Tyr Glu Ser Gly Lys Gly Asp Glu Gln
        195                 200                 205
 
gtg gag aga ttt aag aaa gcg ttt aag gat ttt atg att tta tca atg   672
Val Glu Arg Phe Lys Lys Ala Phe Lys Asp Phe Met Ile Leu Ser Met
    210                 215                 220
 
gag ttt gtg tta tgg gat gca ttt cca att cca tta ttt aaa tgg gtg   720
Glu Phe Val Leu Trp Asp Ala Phe Pro Ile Pro Leu Phe Lys Trp Val
225                 230                 235                 240
 
gat ttt caa ggg cat gtt aag gct atg aaa agg act ttt aaa gat ata   768
Asp Phe Gln Gly His Val Lys Ala Met Lys Arg Thr Phe Lys Asp Ile
                245                 250                 255
 
gat tct gtt ttt cag aat tgg tta gag gaa cat att aag aaa aga gaa   816
Asp Ser Val Phe Gln Asn Trp Leu Glu Glu His Ile Lys Lys Arg Glu
            260                 265                 270
 
aaa ata atg gag gtt ggt aca gaa ggg aat gaa caa gat ttt ata gat   864
Lys Ile Met Glu Val Gly Thr Glu Gly Asn Glu Gln Asp Phe Ile Asp
        275                 280                 285
 
gtg gtg ctt tca aaa atg agt aat gaa tat ctt ggc gaa ggt tac tct   912
Val Val Leu Ser Lys Met Ser Asn Glu Tyr Leu Gly Glu Gly Tyr Ser
    290                 295                 300
 
cgc gat act gtc ata aaa gca aca gta ttt agt ttg gtc ttg gat gca   960
Arg Asp Thr Val Ile Lys Ala Thr Val Phe Ser Leu Val Leu Asp Ala
305                 310                 315                 320
 
gca gac aca gtt gct ctt cac ata aat tgc gga atg gca tta ttg ata   1008
Ala Asp Thr Val Ala Leu His Ile Asn Cys Gly Met Ala Leu Leu Ile
                325                 330                 335
 
aac aat caa aat gcc ttg aag aaa gca caa gaa gag ata gac aca aaa   1056
Asn Asn Gln Asn Ala Leu Lys Lys Ala Gln Glu Glu Ile Asp Thr Lys
            340                 345                 350
 
gtt ggt aag gat aga tgg gta gaa gag agt gat att aag gat ttg gta   1104
Val Gly Lys Asp Arg Trp Val Glu Glu Ser Asp Ile Lys Asp Leu Val
        355                 360                 365
 
tac ctc caa gct att gtt aaa gaa gtg tta cga tta tat cca ccg gga   1152
Tyr Leu Gln Ala Ile Val Lys Glu Val Leu Arg Leu Tyr Pro Pro Gly
    370                 375                 380
 
cct ttg tta gta cca cac gaa aat gta gaa gat tgt gtt gtt agt gga   1200
Pro Leu Leu Val Pro His Glu Asn Val Glu Asp Cys Val Val Ser Gly
385                 390                 395                 400
 
tat cac att cct aaa gga act aga cta ttc gca aat gta atg aaa cta   1248
Tyr His Ile Pro Lys Gly Thr Arg Leu Phe Ala Asn Val Met Lys Leu
                405                 410                 415
 
caa cgt gat cct aaa ctc tgg tca aat cct gat aag ttc aat cca gag   1296
Gln Arg Asp Pro Lys Leu Trp Ser Asn Pro Asp Lys Phe Asn Pro Glu
            420                 425                 430
 
aga ttc atc gct cgt gat att gac ttt cat ggt cag cac tat gag tat   1344
Arg Phe Ile Ala Arg Asp Ile Asp Phe His Gly Gln His Tyr Glu Tyr
        435                 440                 445
 
atc ccg ttt ggt tct gga aga cgc tct tgt ccg ggg atg act tat gca   1392
Ile Pro Phe Gly Ser Gly Arg Arg Ser Cys Pro Gly Met Thr Tyr Ala
    450                 455                 460
 
ttg caa gtg gaa cac cta aca atg gca cat ttg atc cag ggt ttc aat   1440
Leu Gln Val Glu His Leu Thr Met Ala His Leu Ile Gln Gly Phe Asn
465                 470                 475                 480
 
tac aga act cca act gat gag ccc ttg gat atg aaa gaa ggt gca ggc   1488
Tyr Arg Thr Pro Thr Asp Glu Pro Leu Asp Met Lys Glu Gly Ala Gly
                485                 490                 495
 
ata act ata cgt aag gta aat cct gtg aaa gtg ata att acg cct cgc   1536
Ile Thr Ile Arg Lys Val Asn Pro Val Lys Val Ile Ile Thr Pro Arg
            500                 505                 510
 
ttg gca cct gag ctt tat taaaatctaa gatgtttcat cttggttgat          1584
Leu Ala Pro Glu Leu Tyr
        515
 
cattgtttaa tactcctaga tgggtattca tctacctttt ttcaattagt tgtcggtacg 1644
tattttttta atttggtaag tttgtaataa taagtaaaga aggattgtgc taatatataa 1704
tggtgcataa aataattgaa atg                                         1727

<210> 8
<211> 518
<212> PRT
<213> Nicotiana sp.

<400> 8
Met Val Phe Pro Val Glu Ala Ile Val Gly Leu Val Thr Phe Thr Phe
 1               5                  10                  15      
Leu Phe Tyr Phe Leu Trp Thr Lys Lys Ser Gln Lys Pro Ser Lys Pro
            20                  25                  30          
Leu Pro Pro Lys Ile Pro Gly Gly Trp Pro Val Ile Gly His Leu Phe
        35                  40                  45              
Tyr Phe Asp Asp Asp Gly Asp Asp Arg Pro Leu Ala Arg Lys Leu Gly
    50                  55                  60                  
Asp Leu Ala Asp Lys Tyr Gly Pro Val Phe Thr Phe Arg Leu Gly Leu
65                  70                  75                  80  
Pro Leu Val Leu Val Val Ser Ser Tyr Glu Ala Ile Lys Asp Cys Phe
                85                  90                  95      
Ser Thr Asn Asp Ala Ile Phe Ser Asn Arg Pro Ala Phe Leu Tyr Gly
            100                 105                 110         
Glu Tyr Leu Gly Tyr Lys Asn Ala Met Leu Phe Leu Ala Asn Tyr Gly
        115                 120                 125             
Ser Tyr Trp Arg Lys Asn Arg Lys Leu Ile Ile Gln Glu Val Leu Ser
    130                 135                 140                 
Ala Ser Arg Leu Glu Lys Phe Lys His Val Arg Phe Ala Arg Ile Gln
145                 150                 155                 160 
Thr Ser Ile Lys Asn Leu Tyr Thr Arg Ile Asp Gly Asn Ser Ser Thr
                165                 170                 175     
Ile Asn Leu Thr Asp Trp Leu Glu Glu Leu Asn Phe Gly Leu Ile Val
            180                 185                 190         
Lys Met Ile Ala Gly Lys Asn Tyr Glu Ser Gly Lys Gly Asp Glu Gln
        195                 200                 205             
Val Glu Arg Phe Lys Lys Ala Phe Lys Asp Phe Met Ile Leu Ser Met
    210                 215                 220                 
Glu Phe Val Leu Trp Asp Ala Phe Pro Ile Pro Leu Phe Lys Trp Val
225                 230                 235                 240 
Asp Phe Gln Gly His Val Lys Ala Met Lys Arg Thr Phe Lys Asp Ile
                245                 250                 255     
Asp Ser Val Phe Gln Asn Trp Leu Glu Glu His Ile Lys Lys Arg Glu
            260                 265                 270         
Lys Ile Met Glu Val Gly Thr Glu Gly Asn Glu Gln Asp Phe Ile Asp
        275                 280                 285             
Val Val Leu Ser Lys Met Ser Asn Glu Tyr Leu Gly Glu Gly Tyr Ser
    290                 295                 300                 
Arg Asp Thr Val Ile Lys Ala Thr Val Phe Ser Leu Val Leu Asp Ala
305                 310                 315                 320 
Ala Asp Thr Val Ala Leu His Ile Asn Cys Gly Met Ala Leu Leu Ile
                325                 330                 335     
Asn Asn Gln Asn Ala Leu Lys Lys Ala Gln Glu Glu Ile Asp Thr Lys
            340                 345                 350         
Val Gly Lys Asp Arg Trp Val Glu Glu Ser Asp Ile Lys Asp Leu Val
        355                 360                 365             
Tyr Leu Gln Ala Ile Val Lys Glu Val Leu Arg Leu Tyr Pro Pro Gly
    370                 375                 380                 
Pro Leu Leu Val Pro His Glu Asn Val Glu Asp Cys Val Val Ser Gly
385                 390                 395                 400 
Tyr His Ile Pro Lys Gly Thr Arg Leu Phe Ala Asn Val Met Lys Leu
                405                 410                 415     
Gln Arg Asp Pro Lys Leu Trp Ser Asn Pro Asp Lys Phe Asn Pro Glu
            420                 425                 430         
Arg Phe Ile Ala Arg Asp Ile Asp Phe His Gly Gln His Tyr Glu Tyr
        435                 440                 445             
Ile Pro Phe Gly Ser Gly Arg Arg Ser Cys Pro Gly Met Thr Tyr Ala
    450                 455                 460                 
Leu Gln Val Glu His Leu Thr Met Ala His Leu Ile Gln Gly Phe Asn
465                 470                 475                 480 
Tyr Arg Thr Pro Thr Asp Glu Pro Leu Asp Met Lys Glu Gly Ala Gly
                485                 490                 495     
Ile Thr Ile Arg Lys Val Asn Pro Val Lys Val Ile Ile Thr Pro Arg
            500                 505                 510         
Leu Ala Pro Glu Leu Tyr
        515             


<210> 9
<211> 539
<212> DNA
<213> Nicotiana sp.

<220> 
<221> CDS            
<222> (1)...(539)

<400> 9
atg gtt ttt ccc ata gaa gcc ttt gta gga cta gta acc ttc aca ttt   48
Met Val Phe Pro Ile Glu Ala Phe Val Gly Leu Val Thr Phe Thr Phe
 1               5                   10                  15
 
ctc tta tac ttc cta tgg aca aaa aaa tct caa aaa ctt cca aaa ccc   96
Leu Leu Tyr Phe Leu Trp Thr Lys Lys Ser Gln Lys Leu Pro Lys Pro
             20                  25                  30
 
tta cca ccg aaa atc ccc gga gga tgg ccg gta atc ggc cat ctt ttt   144
Leu Pro Pro Lys Ile Pro Gly Gly Trp Pro Val Ile Gly His Leu Phe
         35                  40                  45
 
cac ttc aat aac gac ggc gac gac cgt cca tta gct cga aaa ctc gga   192
His Phe Asn Asn Asp Gly Asp Asp Arg Pro Leu Ala Arg Lys Leu Gly
     50                  55                  60
 
gac tta gct gat aaa tac ggc ccc gtt ttc act ttt cgg cta ggt ctt   240
Asp Leu Ala Asp Lys Tyr Gly Pro Val Phe Thr Phe Arg Leu Gly Leu
 65                  70                  75                  80
 
ccc ctt gtg cta gtt gta agc agt tac gaa gct ata aaa gat tgc ttc   288
Pro Leu Val Leu Val Val Ser Ser Tyr Glu Ala Ile Lys Asp Cys Phe
                 85                  90                  95
 
tct aca aat gat gcc att ttc tcc aat cgt cca gct ttt ctt tat ggc   336
Ser Thr Asn Asp Ala Ile Phe Ser Asn Arg Pro Ala Phe Leu Tyr Gly
            100                 105                 110
 
gaa tac ctt ggc tac agt aat gcc atg cta ttt tga caa aat acg gac   384
Glu Tyr Leu Gly Tyr Ser Asn Ala Met Leu Phe  *  Gln Asn Thr Asp
        115                 120                     125
 
ctt act ggc gaa aaa ata gaa aat tag tca ttc agg aag ttc tct gtg   432
Leu Thr Gly Glu Lys Ile Glu Asn  *  Ser Phe Arg Lys Phe Ser Val
        130                 135                     140
 
cta gtc gtc tcg aaa aat tga agc acg tga gat ttg gtg aaa ttc aga   480
Leu Val Val Ser Lys Asn  *  Ser Thr  *  Asp Leu Val Lys Phe Arg
        145                     150                     155
 
cga gca tta aga att tat aca ctc gaa ttg atg gaa att cga gta cga   528
Arg Ala Leu Arg Ile Tyr Thr Leu Glu Leu Met Glu Ile Arg Val Arg
            160                 165                 170
 
taa atc taa ca                                                    539
 *  Ile  *

 

<210> 10
<211> 173
<212> PRT
<213> Nicotiana sp.

<400> 10
Met Val Phe Pro Ile Glu Ala Phe Val Gly Leu Val Thr Phe Thr Phe
 1               5                  10                  15      
Leu Leu Tyr Phe Leu Trp Thr Lys Lys Ser Gln Lys Leu Pro Lys Pro
            20                  25                  30          
Leu Pro Pro Lys Ile Pro Gly Gly Trp Pro Val Ile Gly His Leu Phe
        35                  40                  45              
His Phe Asn Asn Asp Gly Asp Asp Arg Pro Leu Ala Arg Lys Leu Gly
    50                  55                  60                  
Asp Leu Ala Asp Lys Tyr Gly Pro Val Phe Thr Phe Arg Leu Gly Leu
65                  70                  75                  80  
Pro Leu Val Leu Val Val Ser Ser Tyr Glu Ala Ile Lys Asp Cys Phe
                85                  90                  95      
Ser Thr Asn Asp Ala Ile Phe Ser Asn Arg Pro Ala Phe Leu Tyr Gly
            100                 105                 110         
Glu Tyr Leu Gly Tyr Ser Asn Ala Met Leu Phe Gln Asn Thr Asp Leu
        115                 120                 125             
Thr Gly Glu Lys Ile Glu Asn Ser Phe Arg Lys Phe Ser Val Leu Val
    130                 135                 140                 
Val Ser Lys Asn Ser Thr Asp Leu Val Lys Phe Arg Arg Ala Leu Arg
145                 150                 155                 160 
Ile Tyr Thr Leu Glu Leu Met Glu Ile Arg Val Arg Ile
                165                 170             


<210> 11
<211> 666
<212> DNA
<213> Nicotiana sp.

<220> 
<221> CDS            
<222> (1)...(666)

<400> 11
atg gtt ttt ccc ata gaa gcc att gta gga gca gta acc cta att aca   48
Met Val Phe Pro Ile Glu Ala Ile Val Gly Ala Val Thr Leu Ile Thr
 1               5                   10                  15
 
ttt ctc tta tac ttc cta tgt aca aaa aaa tct caa aaa cat tca aag   96
Phe Leu Leu Tyr Phe Leu Cys Thr Lys Lys Ser Gln Lys His Ser Lys
             20                  25                  30
 
ccc tta cca acg aaa atc ccc gga gga tgg ccg gta atc ggc cat ctt   144
Pro Leu Pro Thr Lys Ile Pro Gly Gly Trp Pro Val Ile Gly His Leu
         35                  40                  45
 
ttc cac ttc aat aac gac ggc gac gac cgt cca ttt gct cga aaa ctc   192
Phe His Phe Asn Asn Asp Gly Asp Asp Arg Pro Phe Ala Arg Lys Leu
     50                  55                  60
 
gga gac tta gct gat aaa tac ggc ccc gtt ttc act ttt cgg cta ggt   240
Gly Asp Leu Ala Asp Lys Tyr Gly Pro Val Phe Thr Phe Arg Leu Gly
 65                  70                  75                  80
 
ctt ccc ctt gtg cta gtt gta agc agt tac gaa gct ata aaa gat tgc   288
Leu Pro Leu Val Leu Val Val Ser Ser Tyr Glu Ala Ile Lys Asp Cys
                 85                  90                  95
 
ttc tct aca aat gac gcc att ttc tcc aat cgt cca gct ttt ctt tac   336
Phe Ser Thr Asn Asp Ala Ile Phe Ser Asn Arg Pro Ala Phe Leu Tyr
            100                 105                 110
 
ggc gaa tac ctt ggc tac aat aat aca atg ctt ttt cta gca aat tac   384
Gly Glu Tyr Leu Gly Tyr Asn Asn Thr Met Leu Phe Leu Ala Asn Tyr
        115                 120                 125
 
gga cct tac tgg cga aaa aat cgt aaa tta gtc att cag gaa gtt ctc   432
Gly Pro Tyr Trp Arg Lys Asn Arg Lys Leu Val Ile Gln Glu Val Leu
    130                 135                 140
 
tct gct agt cgt ctc gaa aaa ttc aaa caa gtg aga ttc acc aga att   480
Ser Ala Ser Arg Leu Glu Lys Phe Lys Gln Val Arg Phe Thr Arg Ile
145                 150                 155                 160
 
caa acg agc att aag aat tta tac act cga att aat gga aat tcg agt   528
Gln Thr Ser Ile Lys Asn Leu Tyr Thr Arg Ile Asn Gly Asn Ser Ser
                165                 170                 175
 
acg ata aat cta agt gat tgg tta gaa gaa ttg aat ttt ggt ctg atc   576
Thr Ile Asn Leu Ser Asp Trp Leu Glu Glu Leu Asn Phe Gly Leu Ile
            180                 185                 190
 
gtg aaa atg atc gct ggg aaa aat tat gaa tcc ggt aaa gga gat gaa   624
Val Lys Met Ile Ala Gly Lys Asn Tyr Glu Ser Gly Lys Gly Asp Glu
        195                 200                 205
 
caa gtg gaa aga ttt aag aat gcg ttt aag gat ttt atg gtt           666
Gln Val Glu Arg Phe Lys Asn Ala Phe Lys Asp Phe Met Val
    210                 215                 220
 


<210> 12
<211> 222
<212> PRT
<213> Nicotiana sp.

<400> 12
Met Val Phe Pro Ile Glu Ala Ile Val Gly Ala Val Thr Leu Ile Thr
 1               5                  10                  15      
Phe Leu Leu Tyr Phe Leu Cys Thr Lys Lys Ser Gln Lys His Ser Lys
            20                  25                  30          
Pro Leu Pro Thr Lys Ile Pro Gly Gly Trp Pro Val Ile Gly His Leu
        35                  40                  45              
Phe His Phe Asn Asn Asp Gly Asp Asp Arg Pro Phe Ala Arg Lys Leu
    50                  55                  60                  
Gly Asp Leu Ala Asp Lys Tyr Gly Pro Val Phe Thr Phe Arg Leu Gly
65                  70                  75                  80  
Leu Pro Leu Val Leu Val Val Ser Ser Tyr Glu Ala Ile Lys Asp Cys
                85                  90                  95      
Phe Ser Thr Asn Asp Ala Ile Phe Ser Asn Arg Pro Ala Phe Leu Tyr
            100                 105                 110         
Gly Glu Tyr Leu Gly Tyr Asn Asn Thr Met Leu Phe Leu Ala Asn Tyr
        115                 120                 125             
Gly Pro Tyr Trp Arg Lys Asn Arg Lys Leu Val Ile Gln Glu Val Leu
    130                 135                 140                 
Ser Ala Ser Arg Leu Glu Lys Phe Lys Gln Val Arg Phe Thr Arg Ile
145                 150                 155                 160 
Gln Thr Ser Ile Lys Asn Leu Tyr Thr Arg Ile Asn Gly Asn Ser Ser
                165                 170                 175     
Thr Ile Asn Leu Ser Asp Trp Leu Glu Glu Leu Asn Phe Gly Leu Ile
            180                 185                 190         
Val Lys Met Ile Ala Gly Lys Asn Tyr Glu Ser Gly Lys Gly Asp Glu
        195                 200                 205             
Gln Val Glu Arg Phe Lys Asn Ala Phe Lys Asp Phe Met Val
    210                 215                 220         


<210> 13
<211> 99
<212> DNA
<213> Artificial Sequence

<220> 
<223> Partial coding sequence selected from SEQ ID NO:1

<400> 13
gacgaggcct tggatatgaa ggaaggtgca ggcataacca tacgtaaggt aaatccagtg 60
gaattgataa taacgcctcg cttggcacct gagctttac                        99

<210> 14
<211> 99
<212> DNA
<213> Artificial Sequence

<220> 
<223> Partial coding sequence selected from SEQ ID NO:3

<400> 14
gacgagccct tggatatgaa ggaaggtgca ggcataacta tacgtaaggt aaatcctgtg 60
gaactgataa tagcgcctcg cctggcacct gagctttat                        99

<210> 15
<211> 99
<212> DNA
<213> Artificial Sequence

<220> 
<223> Partial coding sequence selected from SEQ ID NO:5

<400> 15
gacgagccct tggacatgaa ggaaggtgca ggcataacta tacgtaaggt aaatcctgtg 60
gaactgataa tagcgcctcg cctggcacct gagctttat                        99

<210> 16
<211> 99
<212> DNA
<213> Artificial Sequence

<220> 
<223> Partial coding sequence selected from SEQ ID NO:7

<400> 16
gatgagccct tggatatgaa agaaggtgca ggcataacta tacgtaaggt aaatcctgtg 60
aaagtgataa ttacgcctcg cttggcacct gagctttat                        99

<210> 17
<211> 19
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer
      

<400> 17
cgctctagaa ctagtgatc                                              19

<210> 18
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer
      

<400> 18
tttttgggac aatcagtcaa                                             20

<210> 19
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer
      

<400> 19
gttagattta tcgtactcga att                                         23

<210> 20
<211> 26
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer
      

<400> 20
ttcatttcaa attattttat gcacca                                      26

<210> 21
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer
      

<400> 21
atggtttttc ccatagaagc c                                           21

<210> 22
<211> 17
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer
      

<400> 22
tcgaggtcga cggtatc                                                17

<210> 23
<211> 1118
<212> DNA
<213> Nicotiana sp.

<400> 23
ctgtcattaa agcaacggtg tttgtaagtt catctgtcat ttttcattta ttcactttta 60
ttttgaggag cagacatgtt aataataatt tggagcaact gtaaagttat ctatgtgtac 120
aggttcgagc ctcaggtgca accactaatg cttgtattag attatgttgt ctgcatcata 180
cccctaattg gagtgtggct cttcccgaac cctgcaatgc tggatgctgg atgctttatg 240
tatcagactg acctttttgt taaactatct aaatactaag gatgatttaa taaaaatata 300
gaatggtaaa cagaaaaaga tgagattatt tttggggcta tatggattcg cccgggcttt 360
gggaggtaaa acggtatcta ccagttgaga ctttactcca gaactttatc tcgagagctc 420
tgaataaaaa tgaaatagta tttaccactc caaaatcttt gatggtaaaa agatgagata 480
taacctctta taattgattg aaccacgttg atagaataaa acttctttac tcccattcag 540
cataagaaaa atgaaaccaa acggaattct tctctttttt agggggaaat tccttaattg 600
cttgttgaat atagattcat gtcgttattc tatttttaat aatgatgaaa atcaatatag 660
tcaaagttaa tacttatgtc atttggtttg cggacaagtt atattggaac tatataatac 720
gtctattata gaatagtgat tatttagagg atatacattt tttttggata aatatttgat 780
ttattggatt aaaaatagaa tatacaggta aggtctaaaa cgtgtgtttg cttttacact 840
aaataaactt gacctcgtac aattctaaga aaatatttga aataaatgaa ttattttatt 900
gttaatcaat taaaaaaatc atagtataga tgagatgtgt gcatacttgg caataactat 960
actaactaaa acaaggtatg tgaataattg atattccttt tttaattatt cttttttcca 1020
gagtttggtc ttggatgcag cagacacagt tgctcttcac ataaattggg gaatggcatt 1080
attgataaac aatcaaaagg ccttgacgaa agcacaag                         1118

<210> 24
<211> 998
<212> DNA
<213> Nicotiana sp.

<400> 24
gtaagttcat ctgtcatttt tcatttattc acttttattt tgaggagcag acatgttaat 60
aataatttgg agcaactgta aagttatcta tgtgtacagg ttcgagcctc aggtgcaacc 120
actaatgctt gtattagatt atgttgtctg catcataccc ctaattggag tgtggctctt 180
cccgaaccct gcaatgctgg atgctggatg ctttatgtat cagactgacc tttttgttaa 240
actatctaaa tactaaggat gatttaataa aaatatagaa tggtaaacag aaaaagatga 300
gattattttt ggggctatat ggattcgccc gggctttggg aggtaaaacg gtatctacca 360
gttgagactt tactccagaa ctttatctcg agagctctga ataaaaatga aatagtattt 420
accactccaa aatctttgat ggtaaaaaga tgagatataa cctcttataa ttgattgaac 480
cacgttgata gaataaaact tctttactcc cattcagcat aagaaaaatg aaaccaaacg 540
gaattcttct cttttttagg gggaaattcc ttaattgctt gttgaatata gattcatgtc 600
gttattctat ttttaataat gatgaaaatc aatatagtca aagttaatac ttatgtcatt 660
tggtttgcgg acaagttata ttggaactat ataatacgtc tattatagaa tagtgattat 720
ttagaggata tacatttttt ttggataaat atttgattta ttggattaaa aatagaatat 780
acaggtaagg tctaaaacgt gtgtttgctt ttacactaaa taaacttgac ctcgtacaat 840
tctaagaaaa tatttgaaat aaatgaatta ttttattgtt aatcaattaa aaaaatcata 900
gtatagatga gatgtgtgca tacttggcaa taactatact aactaaaaca aggtatgtga 960
ataattgata ttcctttttt aattattctt ttttccag                         998

<210> 25
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> PCR Primer

<400> 25
ctgtcattaa agcaacggtg                                             20

<210> 26
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> PCR Primer

<400> 26
ggccttgacg aaagcacaag                                             20

<210> 27
<211> 27
<212> DNA
<213> Artificial Sequence

<220> 
<223> oligonucleotide primer

<400> 27
aagctttgac gccatttttt ccaatcg                                     27

<210> 28
<211> 27
<212> DNA
<213> Artificial Sequence

<220> 
<223> oligonucleotide primer

<400> 28
ctcgagtttt ccagcgatca tcttcac                                     27

<210> 29
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> oligonucleotide primer

<400> 29
acgtgatcct aaactctggt ctg                                         23

<210> 30
<211> 19
<212> DNA
<213> Artificial Sequence

<220> 
<223> oligonucleotide primer

<400> 30
gcctgcacct tccttcatg                                              19

<210> 31
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> oligonucleotide primer

<400> 31
cggtaatcgg ccatcttttc                                             20

<210> 32
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> oligonucleotide primer

<400> 32
ccgagttttc gagctaatgg a                                           21

<210> 33
<211> 19
<212> DNA
<213> Artificial Sequence

<220> 
<223> oligonucleotide primer

<400> 33
caatgacgaa cggcgacag                                              19

<210> 34
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> oligonucleotide primer

<400> 34
atggtttttc ccatagaagc c                                           21

<210> 35
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> oligonucleotide primer

<400> 35
tttttgggac aatcagtcaa t                                           21

<210> 36
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> oligonucleotide primer

<400> 36
tgaatgaact gcaggacgag                                             20

<210> 37
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> oligonucleotide primer

<400> 37
aatatcacgg gtagccaacg                                             20
