                         SEQUENCE LISTING

<110>  INTEGRATED DNA TECHNOLOGIES, INC.
 
<120>  DUAL FUNCTION PRIMERS FOR AMPLIFYING DNA AND METHODS OF USE

<130>  013670-9011-WO00

<140>  New International Application
<141>  2006-11-30

<150>  US 11/563,072
<151>  2006-11-24

<160>  47    

<170>  PatentIn version 3.3

<210>  1
<211>  55
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic amplicon to test system

<400>  1
ccagccgtag tcggtagtaa tctatcaagt tctcatcgaa gcggataggc gagcg          55


<210>  2
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic PCR forward primer

<400>  2
cgctcgccta tccgcttc                                                   18


<210>  3
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic PCR Reverse Primer

<400>  3
ccagccgtag tcggtagt                                                   18


<210>  4
<211>  31
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic oligonucleotide probe

<400>  4
ccgttctcga gttcgctcgc ctatccgctt c                                    31


<210>  5
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic oligonucleotide probe

<400>  5
ccgtttcgct cgcctatccg cttc                                            24


<210>  6
<211>  25
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide probe

<400>  6
ccgttctcgc tcgcctatcc gcttc                                           25


<210>  7
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic oligonucleotide probe

<400>  7
ccgttctcgc gctcgcctat ccgcttc                                         27


<210>  8
<211>  29
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic oligonucleotide probe

<400>  8
ccgttctcga gcgctcgcct atccgcttc                                       29


<210>  9
<211>  31
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic oligonucleotide probe

<400>  9
ccgttctcga ggtcgctcgc ctatccgctt c                                    31


<210>  10
<211>  33
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic oligonucleotide probe

<400>  10
ccgttctcga ggtttcgctc gcctatccgc ttc                                  33


<210>  11
<211>  35
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic oligonucleotide probe

<400>  11
ccgttctcga ggtttttcgc tcgcctatcc gcttc                                35


<210>  12
<211>  37
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic oligonucleotide probe

<400>  12
ccgttttctc gaggtttttc gctcgcctat ccgcttc                              37


<210>  13
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic oligonucleotide probe

<400>  13
ccgttctcgc gctcgcctat ccgcttc                                         27


<210>  14
<211>  31
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic oligonucleotide probe

<400>  14
ccgttctcga ggtcgctcgc ctatccgctt c                                    31


<210>  15
<211>  35
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic oligonucleotide probe

<400>  15
ccgttctcga ggtttttcgc tcgcctatcc gcttc                                35


<210>  16
<211>  29
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic oligonucleotide probe

<400>  16
ccgttccgag tcgctcgcct atccgcttc                                       29


<210>  17
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic oligonucleotide probe

<400>  17
tctcgagcgc tcgcctatcc gcttc                                           25


<210>  18
<211>  26
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic oligonucleotide probe

<400>  18
ttctcgagcg ctcgcctatc cgcttc                                          26


<210>  19
<211>  29
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic oligonucleotide probe

<400>  19
ccgttctcga gcgctcgcct atccgcttc                                       29


<210>  20
<211>  31
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic oligonucleotide probe

<400>  20
ccgttctcga ggtcgctcgc ctatccgctt c                                    31


<210>  21
<211>  33
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic oligonucleotide probe

<400>  21
ccgttctcga ggtttcgctc gcctatccgc ttc                                  33


<210>  22
<211>  35
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic oligonucleotide probe

<400>  22
ccgttctcga ggtttttcgc tcgcctatcc gcttc                                35


<210>  23
<211>  37
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic oligonucleotide probe

<400>  23
ccgttctcga ggtttttttc gctcgcctat ccgcttc                              37


<210>  24
<211>  76
<212>  DNA
<213>  Artificial

<220>
<223>  MP48

<400>  24
cagaaggtta tcatctgcca tcgaggcacc cgttcaccct cccccagtga cccggattat     60

ggtctccctc ctcttg                                                     76


<210>  25
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  MP48

<400>  25
cagaaggtta tcatctgcca tcga                                            24


<210>  26
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  MP48

<400>  26
caagaggagg gagaccataa tcc                                             23


<210>  27
<211>  37
<212>  DNA
<213>  Artificial

<220>
<223>  MP48

<400>  27
ccgttctcga ggtcagaagg ttatcatctg ccatcga                              37


<210>  28
<211>  41
<212>  DNA
<213>  Artificial

<220>
<223>  MP48

<400>  28
cgctcgccta tccgcttcag aaggttatca tctgccatcg a                         41


<210>  29
<211>  300
<212>  DNA
<213>  Artificial

<220>
<223>  Target

<400>  29
gaactcaggc caaggtagcg gaggagctgg gcatgcagga gtacgccata accaacgaca     60

agaccaagag gcctgtggcg cttcgcacca agaccttggc ggaccttttg gaatcattta    120

ttgcagcgct gtacattgat aaggatttgg aatatgttca tactttcatg aatgtctgct    180

tctttccacg attgaaagag ttcattttga atcaggattg gaatgacccc aaatcccagc    240

ttcagcagtg ttgcttgaca cttaggacag aaggaaaaga gccagacatt cctctgtaca    300


<210>  30
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  HDrosha For1

<400>  30
accaacgaca agaccaagag                                                 20


<210>  31
<211>  38
<212>  DNA
<213>  Artificial

<220>
<223>  HDrosha Rev1

<400>  31
tcgtggaaag aagcagacat gtctgcttct ttccacga                             38


<210>  32
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  HDrosha Probe 1

<400>  32
accaagacct tggcggacct tt                                              22


<210>  33
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic oligonucleotide probe

<400>  33
tttttttcct ggtttttttt accaacgaca agaccaagag                           40


<210>  34
<211>  36
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic oligonucleotide probe

<400>  34
tttttcctgg ttttttacca acgacaagac caagag                               36


<210>  35
<211>  32
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic oligonucleotide probe

<400>  35
tttcctggtt ttaccaacga caagaccaag ag                                   32


<210>  36
<211>  32
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic oligonucleotide probe

<400>  36
tttcctggtt ttaccaacga caagaccaag ag                                   32


<210>  37
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic oligonucleotide probe

<400>  37
tttttttcct ggtttttttt accaacgaca agaccaagag                           40


<210>  38
<211>  36
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic oligonucleotide probe

<400>  38
tttttcctgg ttttttacca acgacaagac caagag                               36


<210>  39
<211>  32
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic oligonucleotide probe

<400>  39
tttcctggtt ttaccaacga caagaccaag ag                                   32


<210>  40
<211>  32
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic oligonucleotide probe

<400>  40
tttcctggtt ttaccaacga caagaccaag ag                                   32


<210>  41
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Template binding domain

<400>  41
tcggcttcct ccacgtcatc                                                 20


<210>  42
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  Drosha Rev Primer

<400>  42
tcgtggaaag aagcagaca                                                  19


<210>  43
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  chimeric drosha rev primer

<400>  43
tcggcttcct ccacgtcatc ttcgtggaaa gaagcagaca                           40


<210>  44
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  Unmodified FQT probe

<400>  44
tttttttcct ggtttttttt tcggcttcct ccacgtcatc                           40


<210>  45
<211>  41
<212>  DNA
<213>  Artificial

<220>
<223>  5mC-modified


<220>
<221>  misc_feature
<222>  (23)..(23)
<223>  n is a 5-methyl dC

<220>
<221>  misc_feature
<222>  (26)..(26)
<223>  n is a 5-methyl dC

<220>
<221>  misc_feature
<222>  (29)..(30)
<223>  n is a 5-methyl dC

<220>
<221>  misc_feature
<222>  (32)..(33)
<223>  n is a 5-methyl dC

<220>
<221>  misc_feature
<222>  (35)..(35)
<223>  n is a 5-methyl dC

<220>
<221>  misc_feature
<222>  (38)..(38)
<223>  n is a 5-methyl dC

<400>  45
tttttttcct ggtttttttd ttnggnttnn tnnangtnat c                         41


<210>  46
<211>  41
<212>  DNA
<213>  Artificial

<220>
<223>  pdC-modified FQT probe


<220>
<221>  misc_feature
<222>  (23)..(23)
<223>  propynyl-dC

<220>
<221>  misc_feature
<222>  (26)..(26)
<223>  propynyl-dC

<220>
<221>  misc_feature
<222>  (29)..(30)
<223>  propynyl-dC

<220>
<221>  misc_feature
<222>  (32)..(33)
<223>  propynyl-dC

<220>
<221>  misc_feature
<222>  (35)..(35)
<223>  propynyl-dC

<220>
<221>  misc_feature
<222>  (38)..(38)
<223>  propynyl-dC

<400>  46
tttttttcct ggtttttttd ttnggnttnn tnnangtnat c                         41


<210>  47
<211>  41
<212>  DNA
<213>  Artificial

<220>
<223>  LNA modified


<220>
<221>  misc_feature
<222>  (23)..(23)
<223>  locked nucleic acid-dC

<220>
<221>  misc_feature
<222>  (26)..(26)
<223>  locked nucleic acid-dC

<220>
<221>  misc_feature
<222>  (29)..(30)
<223>  locked nucleic acid-dC

<220>
<221>  misc_feature
<222>  (32)..(33)
<223>  locked nucleic acid-dC

<220>
<221>  misc_feature
<222>  (35)..(38)
<223>  locked nucleic acid-dC

<400>  47
tttttttcct ggtttttttd ttnggnttnn tnnangtnat c                         41


