
                                SEQUENCE LISTING

<110> MUSCRF
      SPINALE, Francis G.
      DESCHAMPS, Anne

<120> METHOD AND SYSTEM FOR MEASUREMENT OF IN
  VIVO ENZYME ACTIVITY

<130> 19113.0141P1

<150> 60/855,419          
<151> 2006-10-31  

<160> 11

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<221> MOD_RES        
<222> 1
<223> Pro is bound by DNP moiety   2,4-Dinitrophenyl

<220> 
<221> MOD_RES        
<222> 7
<223> Arg in the D configuration

<220> 
<221> AMIDATION      
<222> 7
<223> NH2

<220> 
<223> Description of Artificial Sequences; note =
      synthetic construct

<400> 1
Pro Leu Gly Leu Trp Ala Xaa
 1               5          


<210> 2
<211> 13
<212> PRT
<213> Artificial Sequence

<220> 
<221> MOD_RES        
<222> 1
<223>  Xaa=Abu and  is preceded and bound by DABCYL
      moiety 4-(4'-dimethylaminophenylazo)  benzoic acid

<220> 
<221> MOD_RES        
<222> 8
<223> Nva

<220> 
<221> MOD_RES        
<222> 11
<223>  Glu is modified with EDANS moiety
      5-(2'aminoethyl)aminonaphthalene-1-sulfonic acid

<220> 
<221> AMIDATION      
<222> 13

<220> 
<223> Description of Artificial Sequences; note =
      synthetic construct

<400> 2
Xaa Arg Pro Leu Pro Val Glu Val Trp Arg Glu Ala Lys
 1               5                  10              


<210> 3
<211> 6
<212> PRT
<213> Artificial Sequence

<220> 
<221> MOD_RES        
<222> 1
<223>  Pro is bound by DNP moiety  2,4-Dinitrophenyl

<220> 
<221> MOD_RES        
<222> 6
<223> Arg is modified with a hydroxyl group

<220> 
<223> Description of Artificial Sequences; note =
      synthetic construct

<400> 3
Pro Leu Ala Leu Trp Arg
 1               5      


<210> 4
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<221> MOD_RES        
<222> 1
<223> Pro is bound by DNP moiety 2,4-Dinitrophenyl

<220> 
<221> MOD_RES        
<222> 7
<223> Arg is modified with a hydroxyl group

<220> 
<223> Description of Artificial Sequences; note =
      synthetic construct

<400> 4
Pro Tyr Ala Tyr Trp Met Arg
 1               5          


<210> 5
<211> 8
<212> PRT
<213> Artificial Sequence

<220> 
<221> MOD_RES        
<222> 1
<223> Arg is bound by DNP moiety 2,4-Dinitrophenyl

<220> 
<221> MOD_RES        
<222> 8
<223> Ser is modified with a hydroxyl group

<220> 
<223> Description of Artificial Sequences; note =
      synthetic construct

<400> 5
Arg Pro Leu Ala Leu Trp Arg Ser
 1               5              


<210> 6
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<221> MOD_RES        
<222> 1
<223> Pro is bound by DNP moiety  2,4-Dinitrophenyl

<220> 
<221> MOD_RES        
<222> 7
<223> Arg is modified with a hydroxyl group

<220> 
<223> Description of Artificial Sequences; note =
      synthetic construct

<400> 6
Pro Leu Ala Tyr Trp Ala Arg
 1               5          


<210> 7
<211> 6
<212> PRT
<213> Artificial Sequence

<220> 
<221> MOD_RES        
<222> 1
<223> Pro is bound by MCA moiety
      (7-methoxycoumarin-4-yl)acetyl

<220> 
<221> VARIANT        
<222> 2
<223> Xaa = Cha

<220> 
<221> MOD_RES        
<222> 4
<223> Nva

<220> 
<221> MOD_RES        
<222> 6
<223> Ala is bound by DPA moiety
      3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl

<220> 
<223> Description of Artificial Sequences; note =
      synthetic construct


<220> 
<221> AMIDATION      
<222> 6

<400> 7
Pro Xaa Gly Val His Ala
 1               5      


<210> 8
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<221> MOD_RES        
<222> 1
<223> Pro is bound by moiety MCA =
      (7-methoxycoumarin-4-yl)acetyl

<220> 
<221> MOD_RES        
<222> 4
<223> Cys is bound by p-OmeBz moiety(
      S-para-methoxybenzyl)

<220> 
<221> MOD_RES        
<222> 7
<223> Arg is bound by Dpa  moiety
      3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl

<220> 
<221> AMIDATION      
<222> 7

<220> 
<223> Description of Artificial Sequences; note =
      synthetic construct

<400> 8
Pro Leu Ala Cys Trp Ala Arg
 1               5          


<210> 9
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<221> MOD_RES        
<222> 1
<223> Pro is bound by DNP moiety 2,4-Dinitrophenyl

<220> 
<221> MOD_RES        
<222> 7
<223> Arg is modified with a hydroxyl group

<220> 
<223> Description of Artificial Sequences; note =
      synthetic construct

<400> 9
Pro Leu Gly Met Trp Ser Arg
 1               5          


<210> 10
<211> 6
<212> PRT
<213> Artificial Sequence

<220> 
<221> MOD_RES        
<222> 1
<223> Ala is bound to and preceded by an energy acceptor
      moiety (X)

<220> 
<221> MOD_RES        
<222> 4
<223> Asn is bound by subsequent   hydrophobic residue
      (Y)

<220> 
<221> VARIANT        
<222> 5
<223> Xaa = Lys or Arg

<220> 
<223> Description of Artificial Sequences; note =
      synthetic construct

<220> 
<221> MOD_RES        
<222> 6
<223> Asp is bound by a subsequent energy donor moiety
      capable of emitting energy (Z)

<400> 10
Ala Gly Glu Asn Xaa Asp
 1               5      


<210> 11
<211> 3
<212> PRT
<213> Artificial Sequence

<220> 
<221> MOD_RES        
<222> 3
<223> Lys is bound to a subsequent fluorophore

<220> 
<223> Description of Artificial Sequences; note =
      synthetic construct

<400> 11
Ala Leu Lys
 1          

