                         SEQUENCE LISTING

<110>  Invitrogen Corporation
 
<120>  Sharply Resolving Labeled Protein Molecular Weight Standards

<130>  IVGN 563 PCT

<140>  TBA
<141>  2007-07-21

<150>  60/820,101
<151>  2006-07-21

<160>  41    

<170>  PatentIn version 3.4

<210>  1
<211>  109
<212>  PRT
<213>  Artificial

<220>
<223>  Escherchia coli

<400>  1

Met Ser Asp Lys Ile Ile His Leu Thr Asp Asp Ser Phe Asp Thr Asp 
1               5                   10                  15      


Val Leu Lys Ala Asp Gly Ala Ile Leu Val Asp Phe Trp Ala Glu Trp 
            20                  25                  30          


Cys Gly Pro Cys Lys Met Ile Ala Pro Ile Leu Asp Glu Ile Ala Asp 
        35                  40                  45              


Glu Tyr Gln Gly Lys Leu Thr Val Ala Lys Leu Asn Ile Asp Gln Asn 
    50                  55                  60                  


Pro Gly Thr Ala Pro Lys Tyr Gly Ile Arg Gly Ile Pro Thr Leu Leu 
65                  70                  75                  80  


Leu Phe Lys Asn Gly Glu Val Ala Ala Thr Lys Val Gly Ala Leu Ser 
                85                  90                  95      


Lys Gly Gln Leu Lys Glu Phe Leu Asp Ala Asn Leu Ala 
            100                 105                 


<210>  2
<211>  105
<212>  PRT
<213>  Artificial

<220>
<223>  Homo sapiens

<400>  2

Met Val Lys Gln Ile Glu Ser Lys Thr Ala Phe Gln Glu Ala Leu Asp 
1               5                   10                  15      


Ala Ala Gly Asp Lys Leu Val Val Val Asp Phe Ser Ala Thr Trp Cys 
            20                  25                  30          


Gly Pro Cys Lys Met Ile Lys Pro Phe Phe His Ser Leu Ser Glu Lys 
        35                  40                  45              


Tyr Ser Asn Val Ile Phe Leu Glu Val Asp Val Asp Asp Cys Gln Asp 
    50                  55                  60                  


Val Ala Ser Glu Cys Glu Val Lys Cys Met Pro Thr Phe Gln Phe Phe 
65                  70                  75                  80  


Lys Lys Gly Gln Lys Val Gly Glu Phe Ser Gly Ala Asn Lys Glu Lys 
                85                  90                  95      


Leu Glu Ala Thr Ile Asn Glu Leu Val 
            100                 105 


<210>  3
<211>  85
<212>  PRT
<213>  Artificial

<220>
<223>  Escherchia coli

<400>  3

Met Gln Thr Val Ile Phe Gly Arg Ser Gly Cys Pro Tyr Cys Val Arg 
1               5                   10                  15      


Ala Lys Asp Leu Ala Glu Lys Leu Ser Asn Glu Arg Asp Asp Phe Gln 
            20                  25                  30          


Tyr Gln Tyr Val Asp Ile Arg Ala Glu Gly Ile Thr Lys Glu Asp Leu 
        35                  40                  45              


Gln Gln Lys Ala Gly Lys Pro Val Glu Thr Val Pro Gln Ile Phe Val 
    50                  55                  60                  


Asp Gln Gln His Ile Gly Gly Tyr Thr Asp Phe Ala Ala Trp Val Lys 
65                  70                  75                  80  


Glu Asn Leu Asp Ala 
                85  


<210>  4
<211>  215
<212>  PRT
<213>  Artificial

<220>
<223>  Escherchia coli

<400>  4

Met Lys Leu Tyr Ile Tyr Asp His Cys Pro Tyr Cys Leu Lys Ala Arg 
1               5                   10                  15      


Met Ile Phe Gly Leu Lys Asn Ile Pro Val Glu Leu His Val Leu Leu 
            20                  25                  30          


Asn Asp Asp Ala Glu Thr Pro Thr Arg Met Val Gly Gln Lys Gln Val 
        35                  40                  45              


Pro Ile Leu Gln Lys Asp Asp Ser Arg Tyr Met Pro Glu Ser Met Asp 
    50                  55                  60                  


Ile Val His Tyr Val Asp Lys Leu Asp Gly Lys Pro Leu Leu Thr Gly 
65                  70                  75                  80  


Lys Arg Ser Pro Ala Ile Glu Glu Trp Leu Arg Lys Val Asn Gly Tyr 
                85                  90                  95      


Ala Asn Lys Leu Leu Leu Pro Arg Phe Ala Lys Ser Ala Phe Asp Glu 
            100                 105                 110         


Phe Ser Thr Pro Ala Ala Arg Lys Tyr Phe Val Asp Lys Lys Glu Ala 
        115                 120                 125             


Ser Ala Gly Asn Phe Ala Asp Leu Leu Ala His Ser Asp Gly Leu Ile 
    130                 135                 140                 


Lys Asn Ile Ser Asp Asp Leu Arg Ala Leu Asp Lys Leu Ile Val Lys 
145                 150                 155                 160 


Pro Asn Ala Val Asn Gly Glu Leu Ser Glu Asp Asp Ile Gln Leu Phe 
                165                 170                 175     


Pro Leu Leu Arg Asn Leu Thr Leu Val Ala Gly Ile Asn Trp Pro Ser 
            180                 185                 190         


Arg Val Ala Asp Tyr Arg Asp Asn Met Ala Lys Gln Thr Gln Ile Asn 
        195                 200                 205             


Leu Leu Ser Ser Met Ala Ile 
    210                 215 


<210>  5
<211>  450
<212>  PRT
<213>  Artificial

<220>
<223>  Escherchia coli

<400>  5

Met Thr Lys His Tyr Asp Tyr Ile Ala Ile Gly Gly Gly Ser Gly Gly 
1               5                   10                  15      


Ile Ala Ser Ile Asn Arg Ala Ala Met Tyr Gly Gln Lys Cys Ala Leu 
            20                  25                  30          


Ile Glu Ala Lys Glu Leu Gly Gly Thr Cys Val Asn Val Gly Cys Val 
        35                  40                  45              


Pro Lys Lys Val Met Trp His Ala Ala Gln Ile Arg Glu Ala Ile His 
    50                  55                  60                  


Met Tyr Gly Pro Asp Tyr Gly Phe Asp Thr Thr Ile Asn Lys Phe Asn 
65                  70                  75                  80  


Trp Glu Thr Leu Ile Ala Ser Arg Thr Ala Tyr Ile Asp Arg Ile His 
                85                  90                  95      


Thr Ser Tyr Glu Asn Val Leu Gly Lys Asn Asn Val Asp Val Ile Lys 
            100                 105                 110         


Gly Phe Ala Arg Phe Val Asp Ala Lys Thr Leu Glu Val Asn Gly Glu 
        115                 120                 125             


Thr Ile Thr Ala Asp His Ile Leu Ile Ala Thr Gly Gly Arg Pro Ser 
    130                 135                 140                 


His Pro Asp Ile Pro Gly Val Glu Tyr Gly Ile Asp Ser Asp Gly Phe 
145                 150                 155                 160 


Phe Ala Leu Pro Ala Leu Pro Glu Arg Val Ala Val Val Gly Ala Gly 
                165                 170                 175     


Tyr Ile Ala Val Glu Leu Ala Gly Val Ile Asn Gly Leu Gly Ala Lys 
            180                 185                 190         


Thr His Leu Phe Val Arg Lys His Ala Pro Leu Arg Ser Phe Asp Pro 
        195                 200                 205             


Met Ile Ser Glu Thr Leu Val Glu Val Met Asn Ala Glu Gly Pro Gln 
    210                 215                 220                 


Leu His Thr Asn Ala Ile Pro Lys Ala Val Val Lys Asn Thr Asp Gly 
225                 230                 235                 240 


Ser Leu Thr Leu Glu Leu Glu Asp Gly Arg Ser Glu Thr Val Asp Cys 
                245                 250                 255     


Leu Ile Trp Ala Ile Gly Arg Glu Pro Ala Asn Asp Asn Ile Asn Leu 
            260                 265                 270         


Glu Ala Ala Gly Val Lys Thr Asn Glu Lys Gly Tyr Ile Val Val Asp 
        275                 280                 285             


Lys Tyr Gln Asn Thr Asn Ile Glu Gly Ile Tyr Ala Val Gly Asp Asn 
    290                 295                 300                 


Thr Gly Ala Val Glu Leu Thr Pro Val Ala Val Ala Ala Gly Arg Arg 
305                 310                 315                 320 


Leu Ser Glu Arg Leu Phe Asn Asn Lys Pro Asp Glu His Leu Asp Tyr 
                325                 330                 335     


Ser Asn Ile Pro Thr Val Val Phe Ser His Pro Pro Ile Gly Thr Val 
            340                 345                 350         


Gly Leu Thr Glu Pro Gln Ala Arg Glu Gln Tyr Gly Asp Asp Gln Val 
        355                 360                 365             


Lys Val Tyr Lys Ser Ser Phe Thr Ala Met Tyr Thr Ala Val Thr Thr 
    370                 375                 380                 


His Arg Gln Pro Cys Arg Met Lys Leu Val Cys Val Gly Ser Glu Glu 
385                 390                 395                 400 


Lys Ile Val Gly Ile His Gly Ile Gly Phe Gly Met Asp Glu Met Leu 
                405                 410                 415     


Gln Gly Phe Ala Val Ala Leu Lys Met Gly Ala Thr Lys Lys Asp Phe 
            420                 425                 430         


Asp Asn Thr Val Ala Ile His Pro Thr Ala Ala Glu Glu Phe Val Thr 
        435                 440                 445             


Met Arg 
    450 


<210>  6
<211>  522
<212>  PRT
<213>  Artificial

<220>
<223>  Homo sapiens

<400>  6

Met Ala Leu Leu Pro Arg Ala Leu Ser Ala Gly Ala Gly Pro Ser Trp 
1               5                   10                  15      


Arg Arg Ala Ala Arg Ala Phe Arg Gly Phe Leu Leu Leu Leu Pro Glu 
            20                  25                  30          


Pro Ala Ala Leu Thr Arg Ala Leu Ser Arg Ala Met Ala Cys Arg Gln 
        35                  40                  45              


Glu Pro Gln Pro Gln Gly Pro Pro Pro Ala Ala Gly Ala Val Ala Ser 
    50                  55                  60                  


Tyr Asp Tyr Leu Val Ile Gly Gly Gly Ser Gly Gly Leu Ala Ser Ala 
65                  70                  75                  80  


Arg Arg Ala Ala Glu Leu Gly Ala Arg Ala Ala Val Val Glu Ser His 
                85                  90                  95      


Lys Leu Gly Gly Thr Cys Val Asn Val Gly Cys Val Pro Lys Lys Val 
            100                 105                 110         


Met Trp Asn Thr Ala Val His Ser Glu Phe Met His Asp His Ala Asp 
        115                 120                 125             


Tyr Gly Phe Pro Ser Cys Glu Gly Lys Phe Asn Trp Arg Val Ile Lys 
    130                 135                 140                 


Glu Lys Arg Asp Ala Tyr Val Ser Arg Leu Asn Ala Ile Tyr Gln Asn 
145                 150                 155                 160 


Asn Leu Thr Lys Ser His Ile Glu Ile Ile Arg Gly His Ala Ala Phe 
                165                 170                 175     


Thr Ser Asp Pro Lys Pro Thr Ile Glu Val Ser Gly Lys Lys Tyr Thr 
            180                 185                 190         


Ala Pro His Ile Leu Ile Ala Thr Gly Gly Met Pro Ser Thr Pro His 
        195                 200                 205             


Glu Ser Gln Ile Pro Gly Ala Ser Leu Gly Ile Thr Ser Asp Gly Phe 
    210                 215                 220                 


Phe Gln Leu Glu Glu Leu Pro Gly Arg Ser Val Ile Val Gly Ala Gly 
225                 230                 235                 240 


Tyr Ile Ala Val Glu Met Ala Gly Ile Leu Ser Ala Leu Gly Ser Lys 
                245                 250                 255     


Thr Ser Leu Met Ile Arg His Asp Lys Val Leu Arg Ser Phe Asp Ser 
            260                 265                 270         


Met Ile Ser Thr Asn Cys Thr Glu Glu Leu Glu Asn Ala Gly Val Glu 
        275                 280                 285             


Val Leu Lys Phe Ser Gln Val Lys Glu Val Lys Lys Thr Leu Ser Gly 
    290                 295                 300                 


Leu Glu Val Ser Met Val Thr Ala Val Pro Gly Arg Leu Pro Val Met 
305                 310                 315                 320 


Thr Met Ile Pro Asp Val Asp Cys Leu Leu Trp Ala Ile Gly Arg Val 
                325                 330                 335     


Pro Asn Thr Lys Asp Leu Ser Leu Asn Lys Leu Gly Ile Gln Thr Asp 
            340                 345                 350         


Asp Lys Gly His Ile Ile Val Asp Glu Phe Gln Asn Thr Asn Val Lys 
        355                 360                 365             


Gly Ile Tyr Ala Val Gly Asp Val Cys Gly Lys Ala Leu Leu Thr Pro 
    370                 375                 380                 


Val Ala Ile Ala Ala Gly Arg Lys Leu Ala His Arg Leu Phe Glu Tyr 
385                 390                 395                 400 


Lys Glu Asp Ser Lys Leu Asp Tyr Asn Asn Ile Pro Thr Val Val Phe 
                405                 410                 415     


Ser His Pro Pro Ile Gly Thr Val Gly Leu Thr Glu Asp Glu Ala Ile 
            420                 425                 430         


His Lys Tyr Gly Ile Glu Asn Val Lys Thr Tyr Ser Thr Ser Phe Thr 
        435                 440                 445             


Pro Met Tyr His Ala Val Thr Lys Arg Lys Thr Lys Cys Val Met Lys 
    450                 455                 460                 


Met Val Cys Ala Asn Lys Glu Glu Lys Val Val Gly Ile His Met Gln 
465                 470                 475                 480 


Gly Leu Gly Cys Asp Glu Met Leu Gln Gly Phe Ala Val Ala Val Lys 
                485                 490                 495     


Met Gly Ala Thr Lys Ala Asp Phe Asp Asn Thr Val Ala Ile His Pro 
            500                 505                 510         


Thr Ser Ser Glu Glu Leu Val Thr Leu Arg 
        515                 520         


<210>  7
<211>  474
<212>  PRT
<213>  Artificial

<220>
<223>  Escherchia coli

<400>  7

Met Ser Thr Glu Ile Lys Thr Gln Val Val Val Leu Gly Ala Gly Pro 
1               5                   10                  15      


Ala Gly Tyr Ser Ala Ala Phe Arg Cys Ala Asp Leu Gly Leu Glu Thr 
            20                  25                  30          


Val Ile Val Glu Arg Tyr Asn Thr Leu Gly Gly Val Cys Leu Asn Val 
        35                  40                  45              


Gly Cys Ile Pro Ser Lys Ala Leu Leu His Val Ala Lys Val Ile Glu 
    50                  55                  60                  


Glu Ala Lys Ala Leu Ala Glu His Gly Ile Val Phe Gly Glu Pro Lys 
65                  70                  75                  80  


Thr Asp Ile Asp Lys Ile Arg Thr Trp Lys Glu Lys Val Ile Asn Gln 
                85                  90                  95      


Leu Thr Gly Gly Leu Ala Gly Met Ala Lys Gly Arg Lys Val Lys Val 
            100                 105                 110         


Val Asn Gly Leu Gly Lys Phe Thr Gly Ala Asn Thr Leu Glu Val Glu 
        115                 120                 125             


Gly Glu Asn Gly Lys Thr Val Ile Asn Phe Asp Asn Ala Ile Ile Ala 
    130                 135                 140                 


Ala Gly Ser Arg Pro Ile Gln Leu Pro Phe Ile Pro His Glu Asp Pro 
145                 150                 155                 160 


Arg Ile Trp Asp Ser Thr Asp Ala Leu Glu Leu Lys Glu Val Pro Glu 
                165                 170                 175     


Arg Leu Leu Val Met Gly Gly Gly Ile Ile Gly Leu Glu Met Gly Thr 
            180                 185                 190         


Val Tyr His Ala Leu Gly Ser Gln Ile Asp Val Val Glu Met Phe Asp 
        195                 200                 205             


Gln Val Ile Pro Ala Ala Asp Lys Asp Ile Val Lys Val Phe Thr Lys 
    210                 215                 220                 


Arg Ile Ser Lys Lys Phe Asn Leu Met Leu Glu Thr Lys Val Thr Ala 
225                 230                 235                 240 


Val Glu Ala Lys Glu Asp Gly Ile Tyr Val Thr Met Glu Gly Lys Lys 
                245                 250                 255     


Ala Pro Ala Glu Pro Gln Arg Tyr Asp Ala Val Leu Val Ala Ile Gly 
            260                 265                 270         


Arg Val Pro Asn Gly Lys Asn Leu Asp Ala Gly Lys Ala Gly Val Glu 
        275                 280                 285             


Val Asp Asp Arg Gly Phe Ile Arg Val Asp Lys Gln Leu Arg Thr Asn 
    290                 295                 300                 


Val Pro His Ile Phe Ala Ile Gly Asp Ile Val Gly Gln Pro Met Leu 
305                 310                 315                 320 


Ala His Lys Gly Val His Glu Gly His Val Ala Ala Glu Val Ile Ala 
                325                 330                 335     


Gly Lys Lys His Tyr Phe Asp Pro Lys Val Ile Pro Ser Ile Ala Tyr 
            340                 345                 350         


Thr Glu Pro Glu Val Ala Trp Val Gly Leu Thr Glu Lys Glu Ala Lys 
        355                 360                 365             


Glu Lys Gly Ile Ser Tyr Glu Thr Ala Thr Phe Pro Trp Ala Ala Ser 
    370                 375                 380                 


Gly Arg Ala Ile Ala Ser Asp Cys Ala Asp Gly Met Thr Lys Leu Ile 
385                 390                 395                 400 


Phe Asp Lys Glu Ser His Arg Val Ile Gly Gly Ala Ile Val Gly Thr 
                405                 410                 415     


Asn Gly Gly Glu Leu Leu Gly Glu Ile Gly Leu Ala Ile Glu Met Gly 
            420                 425                 430         


Cys Asp Ala Glu Asp Ile Ala Leu Thr Ile His Ala His Pro Thr Leu 
        435                 440                 445             


His Glu Ser Val Gly Leu Ala Ala Glu Val Phe Glu Gly Ser Ile Thr 
    450                 455                 460                 


Asp Leu Pro Asn Pro Lys Ala Lys Lys Lys 
465                 470                 


<210>  8
<211>  509
<212>  PRT
<213>  Artificial

<220>
<223>  Homo sapiens

<400>  8

Met Gln Ser Trp Ser Arg Val Tyr Cys Ser Leu Ala Lys Arg Gly His 
1               5                   10                  15      


Phe Asn Arg Ile Ser His Gly Leu Gln Gly Leu Ser Ala Val Pro Leu 
            20                  25                  30          


Arg Thr Tyr Ala Asp Gln Pro Ile Asp Ala Asp Val Thr Val Ile Gly 
        35                  40                  45              


Ser Gly Pro Gly Gly Tyr Val Ala Ala Ile Lys Ala Ala Gln Leu Gly 
    50                  55                  60                  


Phe Lys Thr Val Cys Ile Glu Lys Asn Glu Thr Leu Gly Gly Thr Cys 
65                  70                  75                  80  


Leu Asn Val Gly Cys Ile Pro Ser Lys Ala Leu Leu Asn Asn Ser His 
                85                  90                  95      


Tyr Tyr His Met Ala His Gly Lys Asp Phe Ala Ser Arg Gly Ile Glu 
            100                 105                 110         


Met Ser Glu Val Arg Leu Asn Leu Asp Lys Met Met Glu Gln Lys Ser 
        115                 120                 125             


Thr Ala Val Lys Ala Leu Thr Gly Gly Ile Ala His Leu Phe Lys Gln 
    130                 135                 140                 


Asn Lys Val Val His Val Asn Gly Tyr Gly Lys Ile Thr Gly Lys Asn 
145                 150                 155                 160 


Gln Val Thr Ala Thr Lys Ala Asp Gly Gly Thr Gln Val Ile Asp Thr 
                165                 170                 175     


Lys Asn Ile Leu Ile Ala Thr Gly Ser Glu Val Thr Pro Phe Pro Gly 
            180                 185                 190         


Ile Thr Ile Asp Glu Asp Thr Ile Val Ser Ser Thr Gly Ala Leu Ser 
        195                 200                 205             


Leu Lys Lys Val Pro Glu Lys Met Val Val Ile Gly Ala Gly Val Ile 
    210                 215                 220                 


Gly Val Glu Leu Gly Ser Val Trp Gln Arg Leu Gly Ala Asp Val Thr 
225                 230                 235                 240 


Ala Val Glu Phe Leu Gly His Val Gly Gly Val Gly Ile Asp Met Glu 
                245                 250                 255     


Ile Ser Lys Asn Phe Gln Arg Ile Leu Gln Lys Gln Gly Phe Lys Phe 
            260                 265                 270         


Lys Leu Asn Thr Lys Val Thr Gly Ala Thr Lys Lys Ser Asp Gly Lys 
        275                 280                 285             


Ile Asp Val Ser Ile Glu Ala Ala Ser Gly Gly Lys Ala Glu Val Ile 
    290                 295                 300                 


Thr Cys Asp Val Leu Leu Val Cys Ile Gly Arg Arg Pro Phe Thr Lys 
305                 310                 315                 320 


Asn Leu Gly Leu Glu Glu Leu Gly Ile Glu Leu Asp Pro Arg Gly Arg 
                325                 330                 335     


Ile Pro Val Asn Thr Arg Phe Gln Thr Lys Ile Pro Asn Ile Tyr Ala 
            340                 345                 350         


Ile Gly Asp Val Val Ala Gly Pro Met Leu Ala His Lys Ala Glu Asp 
        355                 360                 365             


Glu Gly Ile Ile Cys Val Glu Gly Met Ala Gly Gly Ala Val His Ile 
    370                 375                 380                 


Asp Tyr Asn Cys Val Pro Ser Val Ile Tyr Thr His Pro Glu Val Ala 
385                 390                 395                 400 


Trp Val Gly Lys Ser Glu Glu Gln Leu Lys Glu Glu Gly Ile Glu Tyr 
                405                 410                 415     


Lys Val Gly Lys Phe Pro Phe Ala Ala Asn Ser Arg Ala Lys Thr Asn 
            420                 425                 430         


Ala Asp Thr Asp Gly Met Val Lys Ile Leu Gly Gln Lys Ser Thr Asp 
        435                 440                 445             


Arg Val Leu Gly Ala His Ile Leu Gly Pro Gly Ala Gly Glu Met Val 
    450                 455                 460                 


Asn Glu Ala Ala Leu Ala Leu Glu Tyr Gly Ala Ser Cys Glu Asp Ile 
465                 470                 475                 480 


Ala Arg Val Cys His Ala His Pro Thr Leu Ser Glu Ala Phe Arg Glu 
                485                 490                 495     


Ala Asn Leu Ala Ala Ser Phe Gly Lys Ser Ile Asn Phe 
            500                 505                 


<210>  9
<211>  279
<212>  DNA
<213>  Artificial

<220>
<223>  Truncated E. coli thioredoxin ORF with C-terminal his tag

<400>  9
atgagcgata aaattattca cctgactgac gacagttttg acacggatgt actcaaagcg     60

gacggggcga tcctcgtcga tttctgggca gagtggtgcg gtccgtgcaa aatgatcgcc    120

ccgattctgg atgaaatcgc tgacgaatat cagggcaaac tgaccgttgc aaaactgaac    180

atcgatcaaa accctggcac tgcgccgaaa tatggcatcc gtggtatccc gactctgctg    240

ctgttcaaaa acggtgaaca ccaccaccac caccactaa                           279


<210>  10
<211>  279
<212>  DNA
<213>  Artificial

<220>
<223>  Modified truncated E. coli thioredoxin ORF lacking lysine with 
       C-terminal his tag

<400>  10

atgagcgata aaattattca cctgactgac gacagttttg acacggatgt actcaaagcg     60

gacggggcga tcctcgtcga tttctgggca gagtggtgcg gtccgtgcaa aatgatcgcc    120

ccgattctgg atgaaatcgc tgacgaatat cagggcaaac tgaccgttgc aaaactgaac    180

atcgatcaaa accctggcac tgcgccgaaa tatggcatcc gtggtatccc gactctgctg    240

ctgttcaaaa acggtgaaca ccaccaccac caccactaa                           279


<210>  11
<211>  92
<212>  PRT
<213>  Artificial

<220>
<223>  Truncated E. coli thioredoxin ORF with C-terminal his tag

<400>  11

Met Ser Asp Lys Ile Ile His Leu Thr Asp Asp Ser Phe Asp Thr Asp 
1               5                   10                  15      


Val Leu Lys Ala Asp Gly Ala Ile Leu Val Asp Phe Trp Ala Glu Trp 
            20                  25                  30          


Cys Gly Pro Cys Lys Met Ile Ala Pro Ile Leu Asp Glu Ile Ala Asp 
        35                  40                  45              


Glu Tyr Gln Gly Lys Leu Thr Val Ala Lys Leu Asn Ile Asp Gln Asn 
    50                  55                  60                  


Pro Gly Thr Ala Pro Lys Tyr Gly Ile Arg Gly Ile Pro Thr Leu Leu 
65                  70                  75                  80  


Leu Phe Lys Asn Gly Glu His His His His His His 
                85                  90          


<210>  12
<211>  92
<212>  PRT
<213>  Artificial

<220>
<223>  Modified truncated E. coli thioredoxin ORF lacking lysine with 
       C-terminal his tag

<400>  12

Met Ser Asp Arg Ile Ile His Leu Thr Asp Asp Ser Phe Asp Thr Asp 
1               5                   10                  15      


Val Leu Arg Ala Asp Gly Ala Ile Leu Val Asp Phe Trp Ala Glu Trp 
            20                  25                  30          


Cys Gly Pro Arg Met Cys Ile Ala Pro Ile Leu Asp Glu Arg Ala Asp 
        35                  40                  45              


Glu Tyr Gln Gly Arg Leu Thr Val Ala Arg Leu Asn Ile Asp Gln Asn 
    50                  55                  60                  


Pro Gly Thr Ala Pro Arg Tyr Gly Ile Arg Gly Ile Pro Thr Leu Leu 
65                  70                  75                  80  


Leu Phe Arg Asn Gly Glu His His His His His His 
                85                  90          


<210>  13
<211>  1595
<212>  DNA
<213>  Artificial

<220>
<223>  BH 6mer ORF having six repeats of modified truncated E. coli 
       thioredoxin ORF lacking lysine and C-terminal his tag

<400>  13
agatctatgt gtgatcgtat tattcacctg actgacgact gctttgacac ggatgtactc     60

cgcgcggacg gggcgcgtct cgtcgatttc tgggcagagt ggtgcggtcc gcgtatgtgt    120

atcgccccga ttctggatga acgtgctgac gaatatcagg gccgcctgac cgttgcacgt    180

ctgaacatcg atcaaaaccc tggcactagc gctatgtgtg atcgtattat tcatctgact    240

gatgactgct ttgacacgga cgtactccgt gcggacgggg cgcgtctcgt cgatttctgg    300

gcagagtggt gcggtccacg tatgtgtatc gcgccgattc tggatgaacg tgcagacgaa    360

tatcaaggcc gcctgaccgt tgcacgcctg aacatcgatc aaaaccctgg tactgcgccg    420

cgctatggca tccgtggcat cccgactctg ctgctgttcc gtaacggcga aggtaccatg    480

tgtgaccgta ttatccacct gactgacgac tgcttcgaca cggatgtact ccgcgcggat    540

ggggcgcgtc tcgtcgactt ctgggcagag tggtgcggtc ctcgtatgtg tatcgcccct    600

attctggatg agcgtgctga cgaatatcag ggtcgcctga ccgttgcacg tctgaatatc    660

gatcaaaacc ctggcactgc accgcgctat ggcatccgtg gtatcccgac tctcctgctg    720

ttccgtaacg gcgaagaatt catgtgtgat cgtatcattc acctgactga cgactgtttt    780

gacacggatg ttctccgcgc ggacggggcg cgtctcgtag atttctgggc agagtggtgc    840

ggcccgcgta tgtgtatcgc cccgattctc gatgaacgtg ctgacgaata tcagggtcgc    900

ctgaccgttg cccgtctgaa catcgatcaa aaccctggca ctgcaccgcg ctatggcatc    960

cgtggtatcc caactctgct gctgttccgt aacggcgaaa ccggtatgtg cgatcgcatt   1020

attcacctga ctgatgactg ctttgacacg gacgtactcc gcgcggacgg ggcgcgcctc   1080

gtcgatttct gggcagagtg gtgcggtccg cgtatgtgta tcgcgccgat tctggatgaa   1140

cgtgcggacg aatatcaggg ccgcctgact gttgcacgtc tgaacatcga ccaaaaccct   1200

ggcactgcgc ctcgctatgg catccgtggt atcccgactc tgctgctctt ccgtaacggc   1260

gaagccggca tgtgtgatcg tatcattcac ctgactgatg actgcttcga cacggatgta   1320

ctccgcgccg acggggcgat cctcgtcgat ttctgggcag aatggtgcgg tccgcgtatg   1380

tgtatcgctc cgatcctgga tgaaatcgct gatgaatatc agggccgcct caccgttgca   1440

cgtctgaata tcgatcaaaa ccctggtact gcgccgcgct atggtatccg tggcatcccg   1500

actcttctgc ttttccgtaa cggcgaagcc ggcaccggtg aattcggtac cagcgctcac   1560

caccaccacc accaccatca tcatcacgtt taaac                              1595


<210>  14
<211>  532
<212>  PRT
<213>  Artificial

<220>
<223>  pTRC BH 60 kDa expression product

<400>  14

Met His Gly Ser Met Cys Asp Arg Ile Ile His Leu Thr Asp Asp Cys 
1               5                   10                  15      


Phe Asp Thr Asp Val Leu Arg Ala Asp Gly Ala Arg Leu Val Asp Phe 
            20                  25                  30          


Trp Ala Glu Trp Cys Gly Pro Arg Met Cys Ile Ala Pro Ile Leu Asp 
        35                  40                  45              


Glu Arg Ala Asp Glu Tyr Gln Gly Arg Leu Thr Val Ala Arg Leu Asn 
    50                  55                  60                  


Ile Asp Gln Asn Pro Gly Thr Ser Ala Met Cys Asp Arg Ile Ile His 
65                  70                  75                  80  


Leu Thr Asp Asp Cys Phe Asp Thr Asp Val Leu Arg Ala Asp Gly Ala 
                85                  90                  95      


Arg Leu Val Asp Phe Trp Ala Glu Trp Cys Gly Pro Arg Met Cys Ile 
            100                 105                 110         


Ala Pro Ile Leu Asp Glu Arg Ala Asp Glu Tyr Gln Gly Arg Leu Thr 
        115                 120                 125             


Val Ala Arg Leu Asn Ile Asp Gln Asn Pro Gly Thr Ala Pro Arg Tyr 
    130                 135                 140                 


Gly Ile Arg Gly Ile Pro Thr Leu Leu Leu Phe Arg Asn Gly Glu Gly 
145                 150                 155                 160 


Thr Met Cys Asp Arg Ile Ile His Leu Thr Asp Asp Cys Phe Asp Thr 
                165                 170                 175     


Asp Val Leu Arg Ala Asp Gly Ala Arg Leu Val Asp Phe Trp Ala Glu 
            180                 185                 190         


Trp Cys Gly Pro Arg Met Cys Ile Ala Pro Ile Leu Asp Glu Arg Ala 
        195                 200                 205             


Asp Glu Tyr Gln Gly Arg Leu Thr Val Ala Arg Leu Asn Ile Asp Gln 
    210                 215                 220                 


Asn Pro Gly Thr Ala Pro Arg Tyr Gly Ile Arg Gly Ile Pro Thr Leu 
225                 230                 235                 240 


Leu Leu Phe Arg Asn Gly Glu Glu Phe Met Cys Asp Arg Ile Ile His 
                245                 250                 255     


Leu Thr Asp Asp Cys Phe Asp Thr Asp Val Leu Arg Ala Asp Gly Ala 
            260                 265                 270         


Arg Leu Val Asp Phe Trp Ala Glu Trp Cys Gly Pro Arg Met Cys Ile 
        275                 280                 285             


Ala Pro Ile Leu Asp Glu Arg Ala Asp Glu Tyr Gln Gly Arg Leu Thr 
    290                 295                 300                 


Val Ala Arg Leu Asn Ile Asp Gln Asn Pro Gly Thr Ala Pro Arg Tyr 
305                 310                 315                 320 


Gly Ile Arg Gly Ile Pro Thr Leu Leu Leu Phe Arg Asn Gly Glu Thr 
                325                 330                 335     


Gly Met Cys Asp Arg Ile Ile His Leu Thr Asp Asp Cys Phe Asp Thr 
            340                 345                 350         


Asp Val Leu Arg Ala Asp Gly Ala Arg Leu Val Asp Phe Trp Ala Glu 
        355                 360                 365             


Trp Cys Gly Pro Arg Met Cys Ile Ala Pro Ile Leu Asp Glu Arg Ala 
    370                 375                 380                 


Asp Glu Tyr Gln Gly Arg Leu Thr Val Ala Arg Leu Asn Ile Asp Gln 
385                 390                 395                 400 


Asn Pro Gly Thr Ala Pro Arg Tyr Gly Ile Arg Gly Ile Pro Thr Leu 
                405                 410                 415     


Leu Leu Phe Arg Asn Gly Glu Ala Gly Met Cys Asp Arg Ile Ile His 
            420                 425                 430         


Leu Thr Asp Asp Cys Phe Asp Thr Asp Val Leu Arg Ala Asp Gly Ala 
        435                 440                 445             


Ile Leu Val Asp Phe Trp Ala Glu Trp Cys Gly Pro Arg Met Cys Ile 
    450                 455                 460                 


Ala Pro Ile Leu Asp Glu Ile Ala Asp Glu Tyr Gln Gly Arg Leu Thr 
465                 470                 475                 480 


Val Ala Arg Leu Asn Ile Asp Gln Asn Pro Gly Thr Ala Pro Arg Tyr 
                485                 490                 495     


Gly Ile Arg Gly Ile Pro Thr Leu Leu Leu Phe Arg Asn Gly Glu Ala 
            500                 505                 510         


Gly Thr Gly Glu Phe Gly Thr Ser Ala His His His His His His His 
        515                 520                 525             


His His His Val 
    530         


<210>  15
<211>  264
<212>  PRT
<213>  Artificial

<220>
<223>  pTRC BH 30 kDa expression product

<400>  15

Met His Gly Ser Met Cys Asp Arg Ile Ile His Leu Thr Asp Asp Cys 
1               5                   10                  15      


Phe Asp Thr Asp Val Leu Arg Ala Asp Gly Ala Arg Leu Val Asp Phe 
            20                  25                  30          


Trp Ala Glu Trp Cys Gly Pro Arg Met Cys Ile Ala Pro Ile Leu Asp 
        35                  40                  45              


Glu Arg Ala Asp Glu Tyr Gln Gly Arg Leu Thr Val Ala Arg Leu Asn 
    50                  55                  60                  


Ile Asp Gln Asn Pro Gly Thr Ser Ala Met Cys Asp Arg Ile Ile His 
65                  70                  75                  80  


Leu Thr Asp Asp Cys Phe Asp Thr Asp Val Leu Arg Ala Asp Gly Ala 
                85                  90                  95      


Arg Leu Val Asp Phe Trp Ala Glu Trp Cys Gly Pro Arg Met Cys Ile 
            100                 105                 110         


Ala Pro Ile Leu Asp Glu Arg Ala Asp Glu Tyr Gln Gly Arg Leu Thr 
        115                 120                 125             


Val Ala Arg Leu Asn Ile Asp Gln Asn Pro Gly Thr Ala Pro Arg Tyr 
    130                 135                 140                 


Gly Ile Arg Gly Ile Pro Thr Leu Leu Leu Phe Arg Asn Gly Glu Gly 
145                 150                 155                 160 


Thr Met Cys Asp Arg Ile Ile His Leu Thr Asp Asp Cys Phe Asp Thr 
                165                 170                 175     


Asp Val Leu Arg Ala Asp Gly Ala Arg Leu Val Asp Phe Trp Ala Glu 
            180                 185                 190         


Trp Cys Gly Pro Arg Met Cys Ile Ala Pro Ile Leu Asp Glu Arg Ala 
        195                 200                 205             


Asp Glu Tyr Gln Gly Arg Leu Thr Val Ala Arg Leu Asn Ile Asp Gln 
    210                 215                 220                 


Asn Pro Gly Thr Ala Pro Arg Tyr Gly Ile Arg Gly Ile Pro Thr Leu 
225                 230                 235                 240 


Leu Leu Phe Arg Asn Gly Glu Glu Phe Gly Thr Ser Ala His His His 
                245                 250                 255     


His His His His His His His Val 
            260                 


<210>  16
<211>  354
<212>  PRT
<213>  Artificial

<220>
<223>  pTRC BH 40 kDa expression product

<400>  16

Met His Gly Ser Met Cys Asp Arg Ile Ile His Leu Thr Asp Asp Cys 
1               5                   10                  15      


Phe Asp Thr Asp Val Leu Arg Ala Asp Gly Ala Arg Leu Val Asp Phe 
            20                  25                  30          


Trp Ala Glu Trp Cys Gly Pro Arg Met Cys Ile Ala Pro Ile Leu Asp 
        35                  40                  45              


Glu Arg Ala Asp Glu Tyr Gln Gly Arg Leu Thr Val Ala Arg Leu Asn 
    50                  55                  60                  


Ile Asp Gln Asn Pro Gly Thr Ser Ala Met Cys Asp Arg Ile Ile His 
65                  70                  75                  80  


Leu Thr Asp Asp Cys Phe Asp Thr Asp Val Leu Arg Ala Asp Gly Ala 
                85                  90                  95      


Arg Leu Val Asp Phe Trp Ala Glu Trp Cys Gly Pro Arg Met Cys Ile 
            100                 105                 110         


Ala Pro Ile Leu Asp Glu Arg Ala Asp Glu Tyr Gln Gly Arg Leu Thr 
        115                 120                 125             


Val Ala Arg Leu Asn Ile Asp Gln Asn Pro Gly Thr Ala Pro Arg Tyr 
    130                 135                 140                 


Gly Ile Arg Gly Ile Pro Thr Leu Leu Leu Phe Arg Asn Gly Glu Gly 
145                 150                 155                 160 


Thr Met Cys Asp Arg Ile Ile His Leu Thr Asp Asp Cys Phe Asp Thr 
                165                 170                 175     


Asp Val Leu Arg Ala Asp Gly Ala Arg Leu Val Asp Phe Trp Ala Glu 
            180                 185                 190         


Trp Cys Gly Pro Arg Met Cys Ile Ala Pro Ile Leu Asp Glu Arg Ala 
        195                 200                 205             


Asp Glu Tyr Gln Gly Arg Leu Thr Val Ala Arg Leu Asn Ile Asp Gln 
    210                 215                 220                 


Asn Pro Gly Thr Ala Pro Arg Tyr Gly Ile Arg Gly Ile Pro Thr Leu 
225                 230                 235                 240 


Leu Leu Phe Arg Asn Gly Glu Glu Phe Met Cys Asp Arg Ile Ile His 
                245                 250                 255     


Leu Thr Asp Asp Cys Phe Asp Thr Asp Val Leu Arg Ala Asp Gly Ala 
            260                 265                 270         


Arg Leu Val Asp Phe Trp Ala Glu Trp Cys Gly Pro Arg Met Cys Ile 
        275                 280                 285             


Ala Pro Ile Leu Asp Glu Arg Ala Asp Glu Tyr Gln Gly Arg Leu Thr 
    290                 295                 300                 


Val Ala Arg Leu Asn Ile Asp Gln Asn Pro Gly Thr Ala Pro Arg Tyr 
305                 310                 315                 320 


Gly Ile Arg Gly Ile Pro Thr Leu Leu Leu Phe Arg Asn Gly Glu Thr 
                325                 330                 335     


Gly Glu Phe Gly Thr Ser Ala His His His His His His His His His 
            340                 345                 350         


His Val 
        


<210>  17
<211>  444
<212>  PRT
<213>  Artificial

<220>
<223>  pTRC BH 50 kDa expression product

<400>  17

Met His Gly Ser Met Cys Asp Arg Ile Ile His Leu Thr Asp Asp Cys 
1               5                   10                  15      


Phe Asp Thr Asp Val Leu Arg Ala Asp Gly Ala Arg Leu Val Asp Phe 
            20                  25                  30          


Trp Ala Glu Trp Cys Gly Pro Arg Met Cys Ile Ala Pro Ile Leu Asp 
        35                  40                  45              


Glu Arg Ala Asp Glu Tyr Gln Gly Arg Leu Thr Val Ala Arg Leu Asn 
    50                  55                  60                  


Ile Asp Gln Asn Pro Gly Thr Ser Ala Met Cys Asp Arg Ile Ile His 
65                  70                  75                  80  


Leu Thr Asp Asp Cys Phe Asp Thr Asp Val Leu Arg Ala Asp Gly Ala 
                85                  90                  95      


Arg Leu Val Asp Phe Trp Ala Glu Trp Cys Gly Pro Arg Met Cys Ile 
            100                 105                 110         


Ala Pro Ile Leu Asp Glu Arg Ala Asp Glu Tyr Gln Gly Arg Leu Thr 
        115                 120                 125             


Val Ala Arg Leu Asn Ile Asp Gln Asn Pro Gly Thr Ala Pro Arg Tyr 
    130                 135                 140                 


Gly Ile Arg Gly Ile Pro Thr Leu Leu Leu Phe Arg Asn Gly Glu Gly 
145                 150                 155                 160 


Thr Met Cys Asp Arg Ile Ile His Leu Thr Asp Asp Cys Phe Asp Thr 
                165                 170                 175     


Asp Val Leu Arg Ala Asp Gly Ala Arg Leu Val Asp Phe Trp Ala Glu 
            180                 185                 190         


Trp Cys Gly Pro Arg Met Cys Ile Ala Pro Ile Leu Asp Glu Arg Ala 
        195                 200                 205             


Asp Glu Tyr Gln Gly Arg Leu Thr Val Ala Arg Leu Asn Ile Asp Gln 
    210                 215                 220                 


Asn Pro Gly Thr Ala Pro Arg Tyr Gly Ile Arg Gly Ile Pro Thr Leu 
225                 230                 235                 240 


Leu Leu Phe Arg Asn Gly Glu Glu Phe Met Cys Asp Arg Ile Ile His 
                245                 250                 255     


Leu Thr Asp Asp Cys Phe Asp Thr Asp Val Leu Arg Ala Asp Gly Ala 
            260                 265                 270         


Arg Leu Val Asp Phe Trp Ala Glu Trp Cys Gly Pro Arg Met Cys Ile 
        275                 280                 285             


Ala Pro Ile Leu Asp Glu Arg Ala Asp Glu Tyr Gln Gly Arg Leu Thr 
    290                 295                 300                 


Val Ala Arg Leu Asn Ile Asp Gln Asn Pro Gly Thr Ala Pro Arg Tyr 
305                 310                 315                 320 


Gly Ile Arg Gly Ile Pro Thr Leu Leu Leu Phe Arg Asn Gly Glu Thr 
                325                 330                 335     


Gly Met Cys Asp Arg Ile Ile His Leu Thr Asp Asp Cys Phe Asp Thr 
            340                 345                 350         


Asp Val Leu Arg Ala Asp Gly Ala Arg Leu Val Asp Phe Trp Ala Glu 
        355                 360                 365             


Trp Cys Gly Pro Arg Met Cys Ile Ala Pro Ile Leu Asp Glu Arg Ala 
    370                 375                 380                 


Asp Glu Tyr Gln Gly Arg Leu Thr Val Ala Arg Leu Asn Ile Asp Gln 
385                 390                 395                 400 


Asn Pro Gly Thr Ala Pro Arg Tyr Gly Ile Arg Gly Ile Pro Thr Leu 
                405                 410                 415     


Leu Leu Phe Arg Asn Gly Glu Ala Gly Thr Gly Glu Phe Gly Thr Ser 
            420                 425                 430         


Ala His His His His His His His His His His Val 
        435                 440                 


<210>  18
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  pTrCHisFOR forward primer

<400>  18
gaggtatata ttaatgtatc g                                               21


<210>  19
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  pBAD_Rev reverse primer

<400>  19
gatttaatct gtatcagg                                                   18


<210>  20
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  50.1_F  forward primer

<400>  20
ccggagatct atgtgtgatc gtattattca                                      30


<210>  21
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  50.1_R reverse primer

<400>  21
ccggctcgag ttcgccgtta cggaaaagca                                      30


<210>  22
<211>  36
<212>  DNA
<213>  Artificial

<220>
<223>  50.2_F forward primer

<400>  22
ccggctcgag atgtgtgatc gtattattca tctgac                               36


<210>  23
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  50.2_R reverse primer

<400>  23
ccggcctagg ttcgccgtta cggaaaagca                                      30


<210>  24
<211>  62
<212>  DNA
<213>  Artificial

<220>
<223>  50.2_10HIS-Pme_R reverse primer

<400>  24
gtttaaacgt gatgatgatg gtggtggtgg tggtggtgtt cgccgttacg gaaaagcaga     60

ag                                                                    62


<210>  25
<211>  36
<212>  DNA
<213>  Artificial

<220>
<223>  50.3_F forward primer

<400>  25
ccggcctagg atgtgtgatc gtattattca tctgac                               36


<210>  26
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  50.3_R reverse primer

<400>  26
ccggcggccg ttcgccgtta cggaaaagca                                      30


<210>  27
<211>  62
<212>  DNA
<213>  Artificial

<220>
<223>  50.3_10HIS-Pme_R reverse primer

<400>  27
gtttaaacgt gatgatgatg gtggtggtgg tggtggtgtt cgccgttacg gaaaagcaga     60

ag                                                                    62


<210>  28
<211>  31
<212>  DNA
<213>  Artificial

<220>
<223>  50.4_F forward primer

<400>  28
ccggcggccg atgtgtgatc gtattattca t                                    31


<210>  29
<211>  62
<212>  DNA
<213>  Artificial

<220>
<223>  50.4_10HIS-Pme_R reverse primer

<400>  29
gtttaaacgt gatgatgatg gtggtggtgg tggtggtgtt cgccgttacg gaaaagcaga     60

ag                                                                    62


<210>  30
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  TA50kd_1F primer

<400>  30
gtgcggtcca cgtatgtg                                                   18


<210>  31
<211>  16
<212>  DNA
<213>  Artificial

<220>
<223>  TA50kd_2F primer

<400>  31
ggcgcgtctc gtcgac                                                     16


<210>  32
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  TA50kd_2R primer

<400>  32
actctgccca gaagtcgac                                                  19


<210>  33
<211>  17
<212>  DNA
<213>  Artificial

<220>
<223>  TA50kd_3F primer

<400>  33
cgaaaccggt atgtgcg                                                    17


<210>  34
<211>  16
<212>  DNA
<213>  Artificial

<220>
<223>  TA50kd_3R primer

<400>  34
cgatcgcaca taccgg                                                     16


<210>  35
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  T7#6 primer

<400>  35
taatacgact cactataggg                                                 20


<210>  36
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  PCRII200F#738 primer

<400>  36
cacacaggaa acagctatga                                                 20


<210>  37
<211>  1314
<212>  DNA
<213>  Artificial

<220>
<223>  pTRC BH 50 kDa ORF

<400>  37
atgtgtgatc gtattattca tctgactgat gactgctttg acacggacgt actccgtgcg     60

gacggggcgc gtctcgtcga tttctgggca gagtggtgcg gtccacgtat gtgtatcgcg    120

ccgattctgg atgaacgtgc agacgaatat caaggccgcc tgaccgttgc acgcctgaac    180

atcgatcaaa accctggtac tgcgccgcgc tatggcatcc gtggcatccc gactctgctg    240

ctgttccgta acggcgaagg taccatgtgt gaccgtatta tccacctgac tgacgactgc    300

ttcgacacgg atgtactccg cgcggatggg gcgcgtctcg tcgacttctg ggcagagtgg    360

tgcggtcctc gtatgtgtat cgcccctatt ctggatgagc gtgctgacga atatcagggt    420

cgcctgaccg ttgcacgtct gaatatcgat caaaaccctg gcactgcacc gcgctatggc    480

atccgtggta tcccgactct cctgctgttc cgtaacggcg aagaattcat gtgtgatcgt    540

atcattcacc tgactgacga ctgttttgac acggatgttc tccgcgcgga cggggcgcgt    600

ctcgtagatt tctgggcaga gtggtgcggc ccgcgtatgt gtatcgcccc gattctcgat    660

gaacgtgctg acgaatatca gggtcgcctg accgttgccc gtctgaacat cgatcaaaac    720

cctggcactg caccgcgcta tggcatccgt ggtatcccaa ctctgctgct gttccgtaac    780

ggcgaaaccg gtatgtgcga tcgcattatt cacctgactg atgactgctt tgacacggac    840

gtactccgcg cggacggggc gcgcctcgtc gatttctggg cagagtggtg cggtccgcgt    900

atgtgtatcg cgccgattct ggatgaacgt gcggacgaat atcagggccg cctgactgtt    960

gcacgtctga acatcgacca aaaccctggc actgcgcctc gctatggcat ccgtggtatc   1020

ccgactctgc tgctcttccg taacggcgaa gccggcatgt gtgatcgtat cattcacctg   1080

actgatgact gcttcgacac ggatgtactc cgcgccgacg gggcgatcct cgtcgatttc   1140

tgggcagaat ggtgcggtcc gcgtatgtgt atcgctccga tcctggatga aatcgctgat   1200

gaatatcagg gccgcctcac cgttgcacgt ctgaatatcg atcaaaaccc tggtactgcg   1260

ccgcgctatg gtatccgtgg catcccgact cttctgcttt tccgtaacgg cgaa         1314


<210>  38
<211>  893
<212>  PRT
<213>  Artificial

<220>
<223>  pTRC 110 kDa expression product

<400>  38

Met His Gly Ser Met Cys Asp Arg Ile Ile His Leu Thr Asp Asp Cys 
1               5                   10                  15      


Phe Asp Thr Asp Val Leu Arg Ala Asp Gly Ala Arg Leu Val Asp Phe 
            20                  25                  30          


Trp Ala Glu Trp Cys Gly Pro Arg Met Cys Ile Ala Pro Ile Leu Asp 
        35                  40                  45              


Glu Arg Ala Asp Glu Tyr Gln Gly Arg Leu Thr Val Ala Arg Leu Asn 
    50                  55                  60                  


Ile Asp Gln Asn Pro Gly Thr Ala Pro Arg Tyr Gly Ile Arg Gly Ile 
65                  70                  75                  80  


Pro Thr Leu Leu Leu Phe Arg Asn Gly Glu Gly Thr Met Cys Asp Arg 
                85                  90                  95      


Ile Ile His Leu Thr Asp Asp Cys Phe Asp Thr Asp Val Leu Arg Ala 
            100                 105                 110         


Asp Gly Ala Arg Leu Val Asp Phe Trp Ala Glu Trp Cys Gly Pro Arg 
        115                 120                 125             


Met Cys Ile Ala Pro Ile Leu Asp Glu Arg Ala Asp Glu Tyr Gln Gly 
    130                 135                 140                 


Arg Leu Thr Val Ala Arg Leu Asn Ile Asp Gln Asn Pro Gly Thr Ala 
145                 150                 155                 160 


Pro Arg Tyr Gly Ile Arg Gly Ile Pro Thr Leu Leu Leu Phe Arg Asn 
                165                 170                 175     


Gly Glu Glu Phe Met Cys Asp Arg Ile Ile His Leu Thr Asp Asp Cys 
            180                 185                 190         


Phe Asp Thr Asp Val Leu Arg Ala Asp Gly Ala Arg Leu Val Asp Phe 
        195                 200                 205             


Trp Ala Glu Trp Cys Gly Pro Arg Met Cys Ile Ala Pro Ile Leu Asp 
    210                 215                 220                 


Glu Arg Ala Asp Glu Tyr Gln Gly Arg Leu Thr Val Ala Arg Leu Asn 
225                 230                 235                 240 


Ile Asp Gln Asn Pro Gly Thr Ala Pro Arg Tyr Gly Ile Arg Gly Ile 
                245                 250                 255     


Pro Thr Leu Leu Leu Phe Arg Asn Gly Glu Thr Gly Met Cys Asp Arg 
            260                 265                 270         


Ile Ile His Leu Thr Asp Asp Cys Phe Asp Thr Asp Val Leu Arg Ala 
        275                 280                 285             


Asp Gly Ala Arg Leu Val Asp Phe Trp Ala Glu Trp Cys Gly Pro Arg 
    290                 295                 300                 


Met Cys Ile Ala Pro Ile Leu Asp Glu Arg Ala Asp Glu Tyr Gln Gly 
305                 310                 315                 320 


Arg Leu Thr Val Ala Arg Leu Asn Ile Asp Gln Asn Pro Gly Thr Ala 
                325                 330                 335     


Pro Arg Tyr Gly Ile Arg Gly Ile Pro Thr Leu Leu Leu Phe Arg Asn 
            340                 345                 350         


Gly Glu Ala Gly Met Cys Asp Arg Ile Ile His Leu Thr Asp Asp Cys 
        355                 360                 365             


Phe Asp Thr Asp Val Leu Arg Ala Asp Gly Ala Ile Leu Val Asp Phe 
    370                 375                 380                 


Trp Ala Glu Trp Cys Gly Pro Arg Met Cys Ile Ala Pro Ile Leu Asp 
385                 390                 395                 400 


Glu Ile Ala Asp Glu Tyr Gln Gly Arg Leu Thr Val Ala Arg Leu Asn 
                405                 410                 415     


Ile Asp Gln Asn Pro Gly Thr Ala Pro Arg Tyr Gly Ile Arg Gly Ile 
            420                 425                 430         


Pro Thr Leu Leu Leu Phe Arg Asn Gly Glu Leu Glu Met Cys Asp Arg 
        435                 440                 445             


Ile Ile His Leu Thr Asp Asp Cys Phe Asp Thr Asp Val Leu Arg Ala 
    450                 455                 460                 


Asp Gly Ala Arg Leu Val Asp Phe Trp Ala Glu Trp Cys Gly Pro Arg 
465                 470                 475                 480 


Met Cys Ile Ala Pro Ile Leu Asp Glu Arg Ala Asp Glu Tyr Gln Gly 
                485                 490                 495     


Arg Leu Thr Val Ala Arg Leu Asn Ile Asp Gln Asn Pro Gly Thr Ala 
            500                 505                 510         


Pro Arg Tyr Gly Ile Arg Gly Ile Pro Thr Leu Leu Leu Phe Arg Asn 
        515                 520                 525             


Gly Glu Gly Thr Met Cys Asp Arg Ile Ile His Leu Thr Asp Asp Cys 
    530                 535                 540                 


Phe Asp Thr Asp Val Leu Arg Ala Asp Gly Ala Arg Leu Val Asp Phe 
545                 550                 555                 560 


Trp Ala Glu Trp Cys Gly Pro Arg Met Cys Ile Ala Pro Ile Leu Asp 
                565                 570                 575     


Glu Arg Ala Asp Glu Tyr Gln Gly Arg Leu Thr Val Ala Arg Leu Asn 
            580                 585                 590         


Ile Asp Gln Asn Pro Gly Thr Ala Pro Arg Tyr Gly Ile Arg Gly Ile 
        595                 600                 605             


Pro Thr Leu Leu Leu Phe Arg Asn Gly Glu Glu Phe Met Cys Asp Arg 
    610                 615                 620                 


Ile Ile His Leu Thr Asp Asp Cys Phe Asp Thr Asp Val Leu Arg Ala 
625                 630                 635                 640 


Asp Gly Ala Arg Leu Val Asp Phe Trp Ala Glu Trp Cys Gly Pro Arg 
                645                 650                 655     


Met Cys Ile Ala Pro Ile Leu Asp Glu Arg Ala Asp Glu Tyr Gln Gly 
            660                 665                 670         


Arg Leu Thr Val Ala Arg Leu Asn Ile Asp Gln Asn Pro Gly Thr Ala 
        675                 680                 685             


Pro Arg Tyr Gly Ile Arg Gly Ile Pro Thr Leu Leu Leu Phe Arg Asn 
    690                 695                 700                 


Gly Glu Thr Gly Met Cys Asp Arg Ile Ile His Leu Thr Asp Asp Cys 
705                 710                 715                 720 


Phe Asp Thr Asp Val Leu Arg Ala Asp Gly Ala Arg Leu Val Asp Phe 
                725                 730                 735     


Trp Ala Glu Trp Cys Gly Pro Arg Met Cys Ile Ala Pro Ile Leu Asp 
            740                 745                 750         


Glu Arg Ala Asp Glu Tyr Gln Gly Arg Leu Thr Val Ala Arg Leu Asn 
        755                 760                 765             


Ile Asp Gln Asn Pro Gly Thr Ala Pro Arg Tyr Gly Ile Arg Gly Ile 
    770                 775                 780                 


Pro Thr Leu Leu Leu Phe Arg Asn Gly Glu Ala Gly Met Cys Asp Arg 
785                 790                 795                 800 


Ile Ile His Leu Thr Asp Asp Cys Phe Asp Thr Asp Val Leu Arg Ala 
                805                 810                 815     


Asp Gly Ala Ile Leu Val Asp Phe Trp Ala Glu Trp Cys Gly Pro Arg 
            820                 825                 830         


Met Cys Ile Ala Pro Ile Leu Asp Glu Ile Ala Asp Glu Tyr Gln Gly 
        835                 840                 845             


Arg Leu Thr Val Ala Arg Leu Asn Ile Asp Gln Asn Pro Gly Thr Ala 
    850                 855                 860                 


Pro Arg Tyr Gly Ile Arg Gly Ile Pro Thr Leu Leu Leu Phe Arg Asn 
865                 870                 875                 880 


Gly Glu His His His His His His His His His His Val 
                885                 890             


<210>  39
<211>  1333
<212>  PRT
<213>  Artificial

<220>
<223>  pTRC 160 kDa predicted expression product

<400>  39

Met His Gly Ser Met Cys Asp Arg Ile Ile His Leu Thr Asp Asp Cys 
1               5                   10                  15      


Phe Asp Thr Asp Val Leu Arg Ala Asp Gly Ala Arg Leu Val Asp Phe 
            20                  25                  30          


Trp Ala Glu Trp Cys Gly Pro Arg Met Cys Ile Ala Pro Ile Leu Asp 
        35                  40                  45              


Glu Arg Ala Asp Glu Tyr Gln Gly Arg Leu Thr Val Ala Arg Leu Asn 
    50                  55                  60                  


Ile Asp Gln Asn Pro Gly Thr Ala Pro Arg Tyr Gly Ile Arg Gly Ile 
65                  70                  75                  80  


Pro Thr Leu Leu Leu Phe Arg Asn Gly Glu Gly Thr Met Cys Asp Arg 
                85                  90                  95      


Ile Ile His Leu Thr Asp Asp Cys Phe Asp Thr Asp Val Leu Arg Ala 
            100                 105                 110         


Asp Gly Ala Arg Leu Val Asp Phe Trp Ala Glu Trp Cys Gly Pro Arg 
        115                 120                 125             


Met Cys Ile Ala Pro Ile Leu Asp Glu Arg Ala Asp Glu Tyr Gln Gly 
    130                 135                 140                 


Arg Leu Thr Val Ala Arg Leu Asn Ile Asp Gln Asn Pro Gly Thr Ala 
145                 150                 155                 160 


Pro Arg Tyr Gly Ile Arg Gly Ile Pro Thr Leu Leu Leu Phe Arg Asn 
                165                 170                 175     


Gly Glu Glu Phe Met Cys Asp Arg Ile Ile His Leu Thr Asp Asp Cys 
            180                 185                 190         


Phe Asp Thr Asp Val Leu Arg Ala Asp Gly Ala Arg Leu Val Asp Phe 
        195                 200                 205             


Trp Ala Glu Trp Cys Gly Pro Arg Met Cys Ile Ala Pro Ile Leu Asp 
    210                 215                 220                 


Glu Arg Ala Asp Glu Tyr Gln Gly Arg Leu Thr Val Ala Arg Leu Asn 
225                 230                 235                 240 


Ile Asp Gln Asn Pro Gly Thr Ala Pro Arg Tyr Gly Ile Arg Gly Ile 
                245                 250                 255     


Pro Thr Leu Leu Leu Phe Arg Asn Gly Glu Thr Gly Met Cys Asp Arg 
            260                 265                 270         


Ile Ile His Leu Thr Asp Asp Cys Phe Asp Thr Asp Val Leu Arg Ala 
        275                 280                 285             


Asp Gly Ala Arg Leu Val Asp Phe Trp Ala Glu Trp Cys Gly Pro Arg 
    290                 295                 300                 


Met Cys Ile Ala Pro Ile Leu Asp Glu Arg Ala Asp Glu Tyr Gln Gly 
305                 310                 315                 320 


Arg Leu Thr Val Ala Arg Leu Asn Ile Asp Gln Asn Pro Gly Thr Ala 
                325                 330                 335     


Pro Arg Tyr Gly Ile Arg Gly Ile Pro Thr Leu Leu Leu Phe Arg Asn 
            340                 345                 350         


Gly Glu Ala Gly Met Cys Asp Arg Ile Ile His Leu Thr Asp Asp Cys 
        355                 360                 365             


Phe Asp Thr Asp Val Leu Arg Ala Asp Gly Ala Ile Leu Val Asp Phe 
    370                 375                 380                 


Trp Ala Glu Trp Cys Gly Pro Arg Met Cys Ile Ala Pro Ile Leu Asp 
385                 390                 395                 400 


Glu Ile Ala Asp Glu Tyr Gln Gly Arg Leu Thr Val Ala Arg Leu Asn 
                405                 410                 415     


Ile Asp Gln Asn Pro Gly Thr Ala Pro Arg Tyr Gly Ile Arg Gly Ile 
            420                 425                 430         


Pro Thr Leu Leu Leu Phe Arg Asn Gly Glu Leu Glu Met Cys Asp Arg 
        435                 440                 445             


Ile Ile His Leu Thr Asp Asp Cys Phe Asp Thr Asp Val Leu Arg Ala 
    450                 455                 460                 


Asp Gly Ala Arg Leu Val Asp Phe Trp Ala Glu Trp Cys Gly Pro Arg 
465                 470                 475                 480 


Met Cys Ile Ala Pro Ile Leu Asp Glu Arg Ala Asp Glu Tyr Gln Gly 
                485                 490                 495     


Arg Leu Thr Val Ala Arg Leu Asn Ile Asp Gln Asn Pro Gly Thr Ala 
            500                 505                 510         


Pro Arg Tyr Gly Ile Arg Gly Ile Pro Thr Leu Leu Leu Phe Arg Asn 
        515                 520                 525             


Gly Glu Gly Thr Met Cys Asp Arg Ile Ile His Leu Thr Asp Asp Cys 
    530                 535                 540                 


Phe Asp Thr Asp Val Leu Arg Ala Asp Gly Ala Arg Leu Val Asp Phe 
545                 550                 555                 560 


Trp Ala Glu Trp Cys Gly Pro Arg Met Cys Ile Ala Pro Ile Leu Asp 
                565                 570                 575     


Glu Arg Ala Asp Glu Tyr Gln Gly Arg Leu Thr Val Ala Arg Leu Asn 
            580                 585                 590         


Ile Asp Gln Asn Pro Gly Thr Ala Pro Arg Tyr Gly Ile Arg Gly Ile 
        595                 600                 605             


Pro Thr Leu Leu Leu Phe Arg Asn Gly Glu Glu Phe Met Cys Asp Arg 
    610                 615                 620                 


Ile Ile His Leu Thr Asp Asp Cys Phe Asp Thr Asp Val Leu Arg Ala 
625                 630                 635                 640 


Asp Gly Ala Arg Leu Val Asp Phe Trp Ala Glu Trp Cys Gly Pro Arg 
                645                 650                 655     


Met Cys Ile Ala Pro Ile Leu Asp Glu Arg Ala Asp Glu Tyr Gln Gly 
            660                 665                 670         


Arg Leu Thr Val Ala Arg Leu Asn Ile Asp Gln Asn Pro Gly Thr Ala 
        675                 680                 685             


Pro Arg Tyr Gly Ile Arg Gly Ile Pro Thr Leu Leu Leu Phe Arg Asn 
    690                 695                 700                 


Gly Glu Thr Gly Met Cys Asp Arg Ile Ile His Leu Thr Asp Asp Cys 
705                 710                 715                 720 


Phe Asp Thr Asp Val Leu Arg Ala Asp Gly Ala Arg Leu Val Asp Phe 
                725                 730                 735     


Trp Ala Glu Trp Cys Gly Pro Arg Met Cys Ile Ala Pro Ile Leu Asp 
            740                 745                 750         


Glu Arg Ala Asp Glu Tyr Gln Gly Arg Leu Thr Val Ala Arg Leu Asn 
        755                 760                 765             


Ile Asp Gln Asn Pro Gly Thr Ala Pro Arg Tyr Gly Ile Arg Gly Ile 
    770                 775                 780                 


Pro Thr Leu Leu Leu Phe Arg Asn Gly Glu Ala Gly Met Cys Asp Arg 
785                 790                 795                 800 


Ile Ile His Leu Thr Asp Asp Cys Phe Asp Thr Asp Val Leu Arg Ala 
                805                 810                 815     


Asp Gly Ala Ile Leu Val Asp Phe Trp Ala Glu Trp Cys Gly Pro Arg 
            820                 825                 830         


Met Cys Ile Ala Pro Ile Leu Asp Glu Ile Ala Asp Glu Tyr Gln Gly 
        835                 840                 845             


Arg Leu Thr Val Ala Arg Leu Asn Ile Asp Gln Asn Pro Gly Thr Ala 
    850                 855                 860                 


Pro Arg Tyr Gly Ile Arg Gly Ile Pro Thr Leu Leu Leu Phe Arg Asn 
865                 870                 875                 880 


Gly Glu Pro Arg Met Cys Asp Arg Ile Ile His Leu Thr Asp Asp Cys 
                885                 890                 895     


Phe Asp Thr Asp Val Leu Arg Ala Asp Gly Ala Arg Leu Val Asp Phe 
            900                 905                 910         


Trp Ala Glu Trp Cys Gly Pro Arg Met Cys Ile Ala Pro Ile Leu Asp 
        915                 920                 925             


Glu Arg Ala Asp Glu Tyr Gln Gly Arg Leu Thr Val Ala Arg Leu Asn 
    930                 935                 940                 


Ile Asp Gln Asn Pro Gly Thr Ala Pro Arg Tyr Gly Ile Arg Gly Ile 
945                 950                 955                 960 


Pro Thr Leu Leu Leu Phe Arg Asn Gly Glu Gly Thr Met Cys Asp Arg 
                965                 970                 975     


Ile Ile His Leu Thr Asp Asp Cys Phe Asp Thr Asp Val Leu Arg Ala 
            980                 985                 990         


Asp Gly Ala Arg Leu Val Asp Phe  Trp Ala Glu Trp Cys  Gly Pro Arg 
        995                 1000                 1005             


Met Cys  Ile Ala Pro Ile Leu  Asp Glu Arg Ala Asp  Glu Tyr Gln 
    1010                 1015                 1020             


Gly Arg  Leu Thr Val Ala Arg  Leu Asn Ile Asp Gln  Asn Pro Gly 
    1025                 1030                 1035             


Thr Ala  Pro Arg Tyr Gly Ile  Arg Gly Ile Pro Thr  Leu Leu Leu 
    1040                 1045                 1050             


Phe Arg  Asn Gly Glu Glu Phe  Met Cys Asp Arg Ile  Ile His Leu 
    1055                 1060                 1065             


Thr Asp  Asp Cys Phe Asp Thr  Asp Val Leu Arg Ala  Asp Gly Ala 
    1070                 1075                 1080             


Arg Leu  Val Asp Phe Trp Ala  Glu Trp Cys Gly Pro  Arg Met Cys 
    1085                 1090                 1095             


Ile Ala  Pro Ile Leu Asp Glu  Arg Ala Asp Glu Tyr  Gln Gly Arg 
    1100                 1105                 1110             


Leu Thr  Val Ala Arg Leu Asn  Ile Asp Gln Asn Pro  Gly Thr Ala 
    1115                 1120                 1125             


Pro Arg  Tyr Gly Ile Arg Gly  Ile Pro Thr Leu Leu  Leu Phe Arg 
    1130                 1135                 1140             


Asn Gly  Glu Thr Gly Met Cys  Asp Arg Ile Ile His  Leu Thr Asp 
    1145                 1150                 1155             


Asp Cys  Phe Asp Thr Asp Val  Leu Arg Ala Asp Gly  Ala Arg Leu 
    1160                 1165                 1170             


Val Asp  Phe Trp Ala Glu Trp  Cys Gly Pro Arg Met  Cys Ile Ala 
    1175                 1180                 1185             


Pro Ile  Leu Asp Glu Arg Ala  Asp Glu Tyr Gln Gly  Arg Leu Thr 
    1190                 1195                 1200             


Val Ala  Arg Leu Asn Ile Asp  Gln Asn Pro Gly Thr  Ala Pro Arg 
    1205                 1210                 1215             


Tyr Gly  Ile Arg Gly Ile Pro  Thr Leu Leu Leu Phe  Arg Asn Gly 
    1220                 1225                 1230             


Glu Ala  Gly Met Cys Asp Arg  Ile Ile His Leu Thr  Asp Asp Cys 
    1235                 1240                 1245             


Phe Asp  Thr Asp Val Leu Arg  Ala Asp Gly Ala Ile  Leu Val Asp 
    1250                 1255                 1260             


Phe Trp  Ala Glu Trp Cys Gly  Pro Arg Met Cys Ile  Ala Pro Ile 
    1265                 1270                 1275             


Leu Asp  Glu Ile Ala Asp Glu  Tyr Gln Gly Arg Leu  Thr Val Ala 
    1280                 1285                 1290             


Arg Leu  Asn Ile Asp Gln Asn  Pro Gly Thr Ala Pro  Arg Tyr Gly 
    1295                 1300                 1305             


Ile Arg  Gly Ile Pro Thr Leu  Leu Leu Phe Arg Asn  Gly Glu His 
    1310                 1315                 1320             


His His  His His His His His  His His Val 
    1325                 1330             


<210>  40
<211>  2721
<212>  DNA
<213>  Artificial

<220>
<223>  truncated lac Z gene sequence

<400>  40
cctaggatga tagatcccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc     60

caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc    120

cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg ctttgcctgg    180

tttccggtac cagaagcggt gccggaaagc tggctggagt gcgatcttcc tgaggccgat    240

actgtcgtcg tcccctcaaa ctggcagatg cacggttacg atgcgcccat ctacaccaac    300

gtaacctatc ccattacggt caatccgccg tttgttccca cggagaatcc gacgggttgt    360

tactcgctca catttaatgt tgatgaaagc tggctacagg aaggccagac gcgaattatt    420

tttgatggcg ttaactcggc gtttcatctg tggtgcaacg ggcgctgggt cggttacggc    480

caggacagtc gtttgccgtc tgaatttgac ctgagcgcat ttttacgcgc cggagaaaac    540

cgcctcgcgg tgatggtgct gcgttggagt gacggcagtt atctggaaga tcaggatatg    600

tggcggatga gcggcatttt ccgtgacgtc tcgttgctgc ataaaccgac tacacaaatc    660

agcgatttcc atgttgccac tcgctttaat gatgatttca gccgcgctgt actggaggct    720

gaagttcaga tgtgcggcga gttgcgtgac tacctacggg taacagtttc tttatggcag    780

ggtgaaacgc aggtcgccag cggcaccgcg cctttcggcg gtgaaattat cgatgagcgt    840

ggtggttatg ccgatcgcgt cacactacgt ctgaacgtcg aaaacccgaa actgtggagc    900

gccgaaatcc cgaatctcta tcgtgcggtg gttgaactgc acaccgccga cggcacgctg    960

attgaagcag aagcctgcga tgtcggtttc cgcgaggtgc ggattgaaaa tggtctgctg   1020

ctgctgaacg gcaagccgtt gctgattcga ggcgttaacc gtcacgagca tcatcctctg   1080

catggtcagg tcatggatga gcagacgatg gtgcaggata tcctgctgat gaagcagaac   1140

aactttaacg ccgtgcgctg ttcgcattat ccgaaccatc cgctgtggta cacgctgtgc   1200

gaccgctacg gcctgtatgt ggtggatgaa gccaatattg aaacccacgg catggtgcca   1260

atgaatcgtc tgaccgatga tccgcgctgg ctaccggcga tgagcgaacg cgtaacgcga   1320

atggtgcagc gcgatcgtaa tcacccgagt gtgatcatct ggtcgctggg gaatgaatca   1380

ggccacggcg ctaatcacga cgcgctgtat cgctggatca aatctgtcga tccttcccgc   1440

ccggtgcagt atgaaggcgg cggagccgac accacggcca ccgatattat ttgcccgatg   1500

tacgcgcgcg tggatgaaga ccagcccttc ccggctgtgc cgaaatggtc catcaaaaaa   1560

tggctttcgc tacctggaga gacgcgcccg ctgatccttt gcgaatacgc ccacgcgatg   1620

ggtaacagtc ttggcggttt cgctaaatac tggcaggcgt ttcgtcagta tccccgttta   1680

cagggcggct tcgtctggga ctgggtggat cagtcgctga ttaaatatga tgaaaacggc   1740

aacccgtggt cggcttacgg cggtgatttt ggcgatacgc cgaacgatcg ccagttctgt   1800

atgaacggtc tggtctttgc cgaccgcacg ccgcatccag cgctgacgga agcaaaacac   1860

cagcagcagt ttttccagtt ccgtttatcc gggcaaacca tcgaagtgac cagcgaatac   1920

ctgttccgtc atagcgataa cgagctcctg cactggatgg tggcgctgga tggtaagccg   1980

ctggcaagcg gtgaagtgcc tctggatgtc gctccacaag gtaaacagtt gattgaactg   2040

cctgaactac cgcagccgga gagcgccggg caactctggc tcacagtacg cgtagtgcaa   2100

ccgaacgcga ccgcatggtc agaagccggg cacatcagcg cctggcagca gtggcgtctg   2160

gcggaaaacc tcagtgtgac gctccccgcc gcgtcccacg ccatcccgca tctgaccacc   2220

agcgaaatgg atttttgcat cgagctgggt aataagcgtt ggcaatttaa ccgccagtca   2280

ggctttcttt cacagatgtg gattggcgat aaaaaacaac tgctgacgcc gctgcgcgat   2340

cagttcaccc gtgcaccgct ggataacgac attggcgtaa gtgaagcgac ccgcattgac   2400

cctaacgcct gggtcgaacg ctggaaggcg gcgggccatt accaggccga agcagcgttg   2460

ttgcagtgca cggcagatac acttgctgat gcggtgctga ttacgaccgc tcacgcgtgg   2520

cagcatcagg ggaaaacctt atttatcagc cggaaaacct accggattga tggtagtggt   2580

caaatggcga ttaccgttga tgttgaagtg gcgagcgata caccgcatcc ggcgcggatt   2640

ggcctgaact gccagctggc gcaggtagca gagcgggtaa actggctcgg attagggccg   2700

caagaaaact atccccctag g                                             2721


<210>  41
<211>  2238
<212>  PRT
<213>  Artificial

<220>
<223>  pTRC 260 kDa expression product

<400>  41

Met His Gly Ser Met Cys Asp Arg Ile Ile His Leu Thr Asp Asp Cys 
1               5                   10                  15      


Phe Asp Thr Asp Val Leu Arg Ala Asp Gly Ala Arg Leu Val Asp Phe 
            20                  25                  30          


Trp Ala Glu Trp Cys Gly Pro Arg Met Cys Ile Ala Pro Ile Leu Asp 
        35                  40                  45              


Glu Arg Ala Asp Glu Tyr Gln Gly Arg Leu Thr Val Ala Arg Leu Asn 
    50                  55                  60                  


Ile Asp Gln Asn Pro Gly Thr Ala Pro Arg Tyr Gly Ile Arg Gly Ile 
65                  70                  75                  80  


Pro Thr Leu Leu Leu Phe Arg Asn Gly Glu Gly Thr Met Cys Asp Arg 
                85                  90                  95      


Ile Ile His Leu Thr Asp Asp Cys Phe Asp Thr Asp Val Leu Arg Ala 
            100                 105                 110         


Asp Gly Ala Arg Leu Val Asp Phe Trp Ala Glu Trp Cys Gly Pro Arg 
        115                 120                 125             


Met Cys Ile Ala Pro Ile Leu Asp Glu Arg Ala Asp Glu Tyr Gln Gly 
    130                 135                 140                 


Arg Leu Thr Val Ala Arg Leu Asn Ile Asp Gln Asn Pro Gly Thr Ala 
145                 150                 155                 160 


Pro Arg Tyr Gly Ile Arg Gly Ile Pro Thr Leu Leu Leu Phe Arg Asn 
                165                 170                 175     


Gly Glu Glu Phe Met Cys Asp Arg Ile Ile His Leu Thr Asp Asp Cys 
            180                 185                 190         


Phe Asp Thr Asp Val Leu Arg Ala Asp Gly Ala Arg Leu Val Asp Phe 
        195                 200                 205             


Trp Ala Glu Trp Cys Gly Pro Arg Met Cys Ile Ala Pro Ile Leu Asp 
    210                 215                 220                 


Glu Arg Ala Asp Glu Tyr Gln Gly Arg Leu Thr Val Ala Arg Leu Asn 
225                 230                 235                 240 


Ile Asp Gln Asn Pro Gly Thr Ala Pro Arg Tyr Gly Ile Arg Gly Ile 
                245                 250                 255     


Pro Thr Leu Leu Leu Phe Arg Asn Gly Glu Thr Gly Met Cys Asp Arg 
            260                 265                 270         


Ile Ile His Leu Thr Asp Asp Cys Phe Asp Thr Asp Val Leu Arg Ala 
        275                 280                 285             


Asp Gly Ala Arg Leu Val Asp Phe Trp Ala Glu Trp Cys Gly Pro Arg 
    290                 295                 300                 


Met Cys Ile Ala Pro Ile Leu Asp Glu Arg Ala Asp Glu Tyr Gln Gly 
305                 310                 315                 320 


Arg Leu Thr Val Ala Arg Leu Asn Ile Asp Gln Asn Pro Gly Thr Ala 
                325                 330                 335     


Pro Arg Tyr Gly Ile Arg Gly Ile Pro Thr Leu Leu Leu Phe Arg Asn 
            340                 345                 350         


Gly Glu Ala Gly Met Cys Asp Arg Ile Ile His Leu Thr Asp Asp Cys 
        355                 360                 365             


Phe Asp Thr Asp Val Leu Arg Ala Asp Gly Ala Ile Leu Val Asp Phe 
    370                 375                 380                 


Trp Ala Glu Trp Cys Gly Pro Arg Met Cys Ile Ala Pro Ile Leu Asp 
385                 390                 395                 400 


Glu Ile Ala Asp Glu Tyr Gln Gly Arg Leu Thr Val Ala Arg Leu Asn 
                405                 410                 415     


Ile Asp Gln Asn Pro Gly Thr Ala Pro Arg Tyr Gly Ile Arg Gly Ile 
            420                 425                 430         


Pro Thr Leu Leu Leu Phe Arg Asn Gly Glu Leu Glu Met Cys Asp Arg 
        435                 440                 445             


Ile Ile His Leu Thr Asp Asp Cys Phe Asp Thr Asp Val Leu Arg Ala 
    450                 455                 460                 


Asp Gly Ala Arg Leu Val Asp Phe Trp Ala Glu Trp Cys Gly Pro Arg 
465                 470                 475                 480 


Met Cys Ile Ala Pro Ile Leu Asp Glu Arg Ala Asp Glu Tyr Gln Gly 
                485                 490                 495     


Arg Leu Thr Val Ala Arg Leu Asn Ile Asp Gln Asn Pro Gly Thr Ala 
            500                 505                 510         


Pro Arg Tyr Gly Ile Arg Gly Ile Pro Thr Leu Leu Leu Phe Arg Asn 
        515                 520                 525             


Gly Glu Gly Thr Met Cys Asp Arg Ile Ile His Leu Thr Asp Asp Cys 
    530                 535                 540                 


Phe Asp Thr Asp Val Leu Arg Ala Asp Gly Ala Arg Leu Val Asp Phe 
545                 550                 555                 560 


Trp Ala Glu Trp Cys Gly Pro Arg Met Cys Ile Ala Pro Ile Leu Asp 
                565                 570                 575     


Glu Arg Ala Asp Glu Tyr Gln Gly Arg Leu Thr Val Ala Arg Leu Asn 
            580                 585                 590         


Ile Asp Gln Asn Pro Gly Thr Ala Pro Arg Tyr Gly Ile Arg Gly Ile 
        595                 600                 605             


Pro Thr Leu Leu Leu Phe Arg Asn Gly Glu Glu Phe Met Cys Asp Arg 
    610                 615                 620                 


Ile Ile His Leu Thr Asp Asp Cys Phe Asp Thr Asp Val Leu Arg Ala 
625                 630                 635                 640 


Asp Gly Ala Arg Leu Val Asp Phe Trp Ala Glu Trp Cys Gly Pro Arg 
                645                 650                 655     


Met Cys Ile Ala Pro Ile Leu Asp Glu Arg Ala Asp Glu Tyr Gln Gly 
            660                 665                 670         


Arg Leu Thr Val Ala Arg Leu Asn Ile Asp Gln Asn Pro Gly Thr Ala 
        675                 680                 685             


Pro Arg Tyr Gly Ile Arg Gly Ile Pro Thr Leu Leu Leu Phe Arg Asn 
    690                 695                 700                 


Gly Glu Thr Gly Met Cys Asp Arg Ile Ile His Leu Thr Asp Asp Cys 
705                 710                 715                 720 


Phe Asp Thr Asp Val Leu Arg Ala Asp Gly Ala Arg Leu Val Asp Phe 
                725                 730                 735     


Trp Ala Glu Trp Cys Gly Pro Arg Met Cys Ile Ala Pro Ile Leu Asp 
            740                 745                 750         


Glu Arg Ala Asp Glu Tyr Gln Gly Arg Leu Thr Val Ala Arg Leu Asn 
        755                 760                 765             


Ile Asp Gln Asn Pro Gly Thr Ala Pro Arg Tyr Gly Ile Arg Gly Ile 
    770                 775                 780                 


Pro Thr Leu Leu Leu Phe Arg Asn Gly Glu Ala Gly Met Cys Asp Arg 
785                 790                 795                 800 


Ile Ile His Leu Thr Asp Asp Cys Phe Asp Thr Asp Val Leu Arg Ala 
                805                 810                 815     


Asp Gly Ala Ile Leu Val Asp Phe Trp Ala Glu Trp Cys Gly Pro Arg 
            820                 825                 830         


Met Cys Ile Ala Pro Ile Leu Asp Glu Ile Ala Asp Glu Tyr Gln Gly 
        835                 840                 845             


Arg Leu Thr Val Ala Arg Leu Asn Ile Asp Gln Asn Pro Gly Thr Ala 
    850                 855                 860                 


Pro Arg Tyr Gly Ile Arg Gly Ile Pro Thr Leu Leu Leu Phe Arg Asn 
865                 870                 875                 880 


Gly Glu Pro Arg Met Ile Asp Pro Val Val Leu Gln Arg Arg Asp Trp 
                885                 890                 895     


Glu Asn Pro Gly Val Thr Gln Leu Asn Arg Leu Ala Ala His Pro Pro 
            900                 905                 910         


Phe Ala Ser Trp Arg Asn Ser Glu Glu Ala Arg Thr Asp Arg Pro Ser 
        915                 920                 925             


Gln Gln Leu Arg Ser Leu Asn Gly Glu Trp Arg Phe Ala Trp Phe Pro 
    930                 935                 940                 


Val Pro Glu Ala Val Pro Glu Ser Trp Leu Glu Cys Asp Leu Pro Glu 
945                 950                 955                 960 


Ala Asp Thr Val Val Val Pro Ser Asn Trp Gln Met His Gly Tyr Asp 
                965                 970                 975     


Ala Pro Ile Tyr Thr Asn Val Thr Tyr Pro Ile Thr Val Asn Pro Pro 
            980                 985                 990         


Phe Val Pro Thr Glu Asn Pro Thr  Gly Cys Tyr Ser Leu  Thr Phe Asn 
        995                 1000                 1005             


Val Asp  Glu Ser Trp Leu Gln  Glu Gly Gln Thr Arg  Ile Ile Phe 
    1010                 1015                 1020             


Asp Gly  Val Asn Ser Ala Phe  His Leu Trp Cys Asn  Gly Arg Trp 
    1025                 1030                 1035             


Val Gly  Tyr Gly Gln Asp Ser  Arg Leu Pro Ser Glu  Phe Asp Leu 
    1040                 1045                 1050             


Ser Ala  Phe Leu Arg Ala Gly  Glu Asn Arg Leu Ala  Val Met Val 
    1055                 1060                 1065             


Leu Arg  Trp Ser Asp Gly Ser  Tyr Leu Glu Asp Gln  Asp Met Trp 
    1070                 1075                 1080             


Arg Met  Ser Gly Ile Phe Arg  Asp Val Ser Leu Leu  His Lys Pro 
    1085                 1090                 1095             


Thr Thr  Gln Ile Ser Asp Phe  His Val Ala Thr Arg  Phe Asn Asp 
    1100                 1105                 1110             


Asp Phe  Ser Arg Ala Val Leu  Glu Ala Glu Val Gln  Met Cys Gly 
    1115                 1120                 1125             


Glu Leu  Arg Asp Tyr Leu Arg  Val Thr Val Ser Leu  Trp Gln Gly 
    1130                 1135                 1140             


Glu Thr  Gln Val Ala Ser Gly  Thr Ala Pro Phe Gly  Gly Glu Ile 
    1145                 1150                 1155             


Ile Asp  Glu Arg Gly Gly Tyr  Ala Asp Arg Val Thr  Leu Arg Leu 
    1160                 1165                 1170             


Asn Val  Glu Asn Pro Lys Leu  Trp Ser Ala Glu Ile  Pro Asn Leu 
    1175                 1180                 1185             


Tyr Arg  Ala Val Val Glu Leu  His Thr Ala Asp Gly  Thr Leu Ile 
    1190                 1195                 1200             


Glu Ala  Glu Ala Cys Asp Val  Gly Phe Arg Glu Val  Arg Ile Glu 
    1205                 1210                 1215             


Asn Gly  Leu Leu Leu Leu Asn  Gly Lys Pro Leu Leu  Ile Arg Gly 
    1220                 1225                 1230             


Val Asn  Arg His Glu His His  Pro Leu His Gly Gln  Val Met Asp 
    1235                 1240                 1245             


Glu Gln  Thr Met Val Gln Asp  Ile Leu Leu Met Lys  Gln Asn Asn 
    1250                 1255                 1260             


Phe Asn  Ala Val Arg Cys Ser  His Tyr Pro Asn His  Pro Leu Trp 
    1265                 1270                 1275             


Tyr Thr  Leu Cys Asp Arg Tyr  Gly Leu Tyr Val Val  Asp Glu Ala 
    1280                 1285                 1290             


Asn Ile  Glu Thr His Gly Met  Val Pro Met Asn Arg  Leu Thr Asp 
    1295                 1300                 1305             


Asp Pro  Arg Trp Leu Pro Ala  Met Ser Glu Arg Val  Thr Arg Met 
    1310                 1315                 1320             


Val Gln  Arg Asp Arg Asn His  Pro Ser Val Ile Ile  Trp Ser Leu 
    1325                 1330                 1335             


Gly Asn  Glu Ser Gly His Gly  Ala Asn His Asp Ala  Leu Tyr Arg 
    1340                 1345                 1350             


Trp Ile  Lys Ser Val Asp Pro  Ser Arg Pro Val Gln  Tyr Glu Gly 
    1355                 1360                 1365             


Gly Gly  Ala Asp Thr Thr Ala  Thr Asp Ile Ile Cys  Pro Met Tyr 
    1370                 1375                 1380             


Ala Arg  Val Asp Glu Asp Gln  Pro Phe Pro Ala Val  Pro Lys Trp 
    1385                 1390                 1395             


Ser Ile  Lys Lys Trp Leu Ser  Leu Pro Gly Glu Thr  Arg Pro Leu 
    1400                 1405                 1410             


Ile Leu  Cys Glu Tyr Ala His  Ala Met Gly Asn Ser  Leu Gly Gly 
    1415                 1420                 1425             


Phe Ala  Lys Tyr Trp Gln Ala  Phe Arg Gln Tyr Pro  Arg Leu Gln 
    1430                 1435                 1440             


Gly Gly  Phe Val Trp Asp Trp  Val Asp Gln Ser Leu  Ile Lys Tyr 
    1445                 1450                 1455             


Asp Glu  Asn Gly Asn Pro Trp  Ser Ala Tyr Gly Gly  Asp Phe Gly 
    1460                 1465                 1470             


Asp Thr  Pro Asn Asp Arg Gln  Phe Cys Met Asn Gly  Leu Val Phe 
    1475                 1480                 1485             


Ala Asp  Arg Thr Pro His Pro  Ala Leu Thr Glu Ala  Lys His Gln 
    1490                 1495                 1500             


Gln Gln  Phe Phe Gln Phe Arg  Leu Ser Gly Gln Thr  Ile Glu Val 
    1505                 1510                 1515             


Thr Ser  Glu Tyr Leu Phe Arg  His Ser Asp Asn Glu  Leu Leu His 
    1520                 1525                 1530             


Trp Met  Val Ala Leu Asp Gly  Lys Pro Leu Ala Ser  Gly Glu Val 
    1535                 1540                 1545             


Pro Leu  Asp Val Ala Pro Gln  Gly Lys Gln Leu Ile  Glu Leu Pro 
    1550                 1555                 1560             


Glu Leu  Pro Gln Pro Glu Ser  Ala Gly Gln Leu Trp  Leu Thr Val 
    1565                 1570                 1575             


Arg Val  Val Gln Pro Asn Ala  Thr Ala Trp Ser Glu  Ala Gly His 
    1580                 1585                 1590             


Ile Ser  Ala Trp Gln Gln Trp  Arg Leu Ala Glu Asn  Leu Ser Val 
    1595                 1600                 1605             


Thr Leu  Pro Ala Ala Ser His  Ala Ile Pro His Leu  Thr Thr Ser 
    1610                 1615                 1620             


Glu Met  Asp Phe Cys Ile Glu  Leu Gly Asn Lys Arg  Trp Gln Phe 
    1625                 1630                 1635             


Asn Arg  Gln Ser Gly Phe Leu  Ser Gln Met Trp Ile  Gly Asp Lys 
    1640                 1645                 1650             


Lys Gln  Leu Leu Thr Pro Leu  Arg Asp Gln Phe Thr  Arg Ala Pro 
    1655                 1660                 1665             


Leu Asp  Asn Asp Ile Gly Val  Ser Glu Ala Thr Arg  Ile Asp Pro 
    1670                 1675                 1680             


Asn Ala  Trp Val Glu Arg Trp  Lys Ala Ala Gly His  Tyr Gln Ala 
    1685                 1690                 1695             


Glu Ala  Ala Leu Leu Gln Cys  Thr Ala Asp Thr Leu  Ala Asp Ala 
    1700                 1705                 1710             


Val Leu  Ile Thr Thr Ala His  Ala Trp Gln His Gln  Gly Lys Thr 
    1715                 1720                 1725             


Leu Phe  Ile Ser Arg Lys Thr  Tyr Arg Ile Asp Gly  Ser Gly Gln 
    1730                 1735                 1740             


Met Ala  Ile Thr Val Asp Val  Glu Val Ala Ser Asp  Thr Pro His 
    1745                 1750                 1755             


Pro Ala  Arg Ile Gly Leu Asn  Cys Gln Leu Ala Gln  Val Ala Glu 
    1760                 1765                 1770             


Arg Val  Asn Trp Leu Gly Leu  Gly Pro Gln Glu Asn  Tyr Pro Pro 
    1775                 1780                 1785             


Arg Met  Cys Asp Arg Ile Ile  His Leu Thr Asp Asp  Cys Phe Asp 
    1790                 1795                 1800             


Thr Asp  Val Leu Arg Ala Asp  Gly Ala Arg Leu Val  Asp Phe Trp 
    1805                 1810                 1815             


Ala Glu  Trp Cys Gly Pro Arg  Met Cys Ile Ala Pro  Ile Leu Asp 
    1820                 1825                 1830             


Glu Arg  Ala Asp Glu Tyr Gln  Gly Arg Leu Thr Val  Ala Arg Leu 
    1835                 1840                 1845             


Asn Ile  Asp Gln Asn Pro Gly  Thr Ala Pro Arg Tyr  Gly Ile Arg 
    1850                 1855                 1860             


Gly Ile  Pro Thr Leu Leu Leu  Phe Arg Asn Gly Glu  Gly Thr Met 
    1865                 1870                 1875             


Cys Asp  Arg Ile Ile His Leu  Thr Asp Asp Cys Phe  Asp Thr Asp 
    1880                 1885                 1890             


Val Leu  Arg Ala Asp Gly Ala  Arg Leu Val Asp Phe  Trp Ala Glu 
    1895                 1900                 1905             


Trp Cys  Gly Pro Arg Met Cys  Ile Ala Pro Ile Leu  Asp Glu Arg 
    1910                 1915                 1920             


Ala Asp  Glu Tyr Gln Gly Arg  Leu Thr Val Ala Arg  Leu Asn Ile 
    1925                 1930                 1935             


Asp Gln  Asn Pro Gly Thr Ala  Pro Arg Tyr Gly Ile  Arg Gly Ile 
    1940                 1945                 1950             


Pro Thr  Leu Leu Leu Phe Arg  Asn Gly Glu Glu Phe  Met Cys Asp 
    1955                 1960                 1965             


Arg Ile  Ile His Leu Thr Asp  Asp Cys Phe Asp Thr  Asp Val Leu 
    1970                 1975                 1980             


Arg Ala  Asp Gly Ala Arg Leu  Val Asp Phe Trp Ala  Glu Trp Cys 
    1985                 1990                 1995             


Gly Pro  Arg Met Cys Ile Ala  Pro Ile Leu Asp Glu  Arg Ala Asp 
    2000                 2005                 2010             


Glu Tyr  Gln Gly Arg Leu Thr  Val Ala Arg Leu Asn  Ile Asp Gln 
    2015                 2020                 2025             


Asn Pro  Gly Thr Ala Pro Arg  Tyr Gly Ile Arg Gly  Ile Pro Thr 
    2030                 2035                 2040             


Leu Leu  Leu Phe Arg Asn Gly  Glu Thr Gly Met Cys  Asp Arg Ile 
    2045                 2050                 2055             


Ile His  Leu Thr Asp Asp Cys  Phe Asp Thr Asp Val  Leu Arg Ala 
    2060                 2065                 2070             


Asp Gly  Ala Arg Leu Val Asp  Phe Trp Ala Glu Trp  Cys Gly Pro 
    2075                 2080                 2085             


Arg Met  Cys Ile Ala Pro Ile  Leu Asp Glu Arg Ala  Asp Glu Tyr 
    2090                 2095                 2100             


Gln Gly  Arg Leu Thr Val Ala  Arg Leu Asn Ile Asp  Gln Asn Pro 
    2105                 2110                 2115             


Gly Thr  Ala Pro Arg Tyr Gly  Ile Arg Gly Ile Pro  Thr Leu Leu 
    2120                 2125                 2130             


Leu Phe  Arg Asn Gly Glu Ala  Gly Met Cys Asp Arg  Ile Ile His 
    2135                 2140                 2145             


Leu Thr  Asp Asp Cys Phe Asp  Thr Asp Val Leu Arg  Ala Asp Gly 
    2150                 2155                 2160             


Ala Ile  Leu Val Asp Phe Trp  Ala Glu Trp Cys Gly  Pro Arg Met 
    2165                 2170                 2175             


Cys Ile  Ala Pro Ile Leu Asp  Glu Ile Ala Asp Glu  Tyr Gln Gly 
    2180                 2185                 2190             


Arg Leu  Thr Val Ala Arg Leu  Asn Ile Asp Gln Asn  Pro Gly Thr 
    2195                 2200                 2205             


Ala Pro  Arg Tyr Gly Ile Arg  Gly Ile Pro Thr Leu  Leu Leu Phe 
    2210                 2215                 2220             


Arg Asn  Gly Glu His His His  His His His His His  His His Val 
    2225                 2230                 2235             


