                         SEQUENCE LISTING



<110>  BASF Plant Science GmbH



<120>  Verfahren zur Herstellung von mehrfach ungsttigten Fettsuren in transgenen Organismen



<130>  



<140>  

<141>  



<160>  44    



<170>  PatentIn version 3.1

<210> 1
<211> 1696
<212> DNA
<213> Ostreococcus lucimarinus

<220>
<223> Delta-12 desaturase

<220>
<221> CDS
<222> (374)..(1462)

<400> 1
cctttgtaac gccccgattc gttcgcgcgt ttcgctcgcc tcgcgtccgc cttcgcgcct     60
                                                                  

cgcgggataa ggctctcccg tcgcgtcgtc gcgttcgcac tcaccggcgc tcgcgcgtcg     120
                                                                  

accgctttcc cgcggcaccg cgatccacga acgacagtcg cgcgtcatgc tccgcgctct     180
                                                                  

gacgctcccg cgcgctcgcg cgctcccgcg cgccgcgttc tctcgctcga cgacgtcacg     240
                                                                  

tcgcgatcgc gcgcgcgtcg cccgcgccgc gcgcgccgac gccgcgacga cctacgacac     300
                                                                  

gtcgcgacac gaacaactgc gctcgggctt gaacgccaag gcgctgaacg ccgaggcgaa     360
                                                                  

gacgttcccg acg atg caa gag gtg ctg cgc gcg atc ccg agg gag tgc        409
               Met Gln Glu Val Leu Arg Ala Ile Pro Arg Glu Cys 
                 1               5                  10
ttc gag cgg gac acg gga aag tcg ctc gcg tac gcg gcg tgc tcg acg       457
Phe Glu Arg Asp Thr Gly Lys Ser Leu Ala Tyr Ala Ala Cys Ser Thr 
         15                  20                  25  
gcg atc acg ctg gcg tgc ggc gcg ctg gcg tgg gcg tgc gtg ccg gtg       505
Ala Ile Thr Leu Ala Cys Gly Ala Leu Ala Trp Ala Cys Val Pro Val 
     30                  35                  40  
acg gcg gcg tat tgg ccg gtg tgg gtg gcg tac gcg ttc gtg acg gga       553
Thr Ala Ala Tyr Trp Pro Val Trp Val Ala Tyr Ala Phe Val Thr Gly 
 45                  50                  55                  60   
acg gcg gcg acc ggg tgc tgg gtc gcg gcg cac gag tgc gga cac ggc       601
Thr Ala Ala Thr Gly Cys Trp Val Ala Ala His Glu Cys Gly His Gly 
                 65                  70                  75  
gcg ttt agc gac gat aaa acg att caa gac gcg gtg gga tac gcg ctg       649
Ala Phe Ser Asp Asp Lys Thr Ile Gln Asp Ala Val Gly Tyr Ala Leu 
             80                  85                  90  
cac tcg ttg ttg ttg gtg ccg tat ttt tcg tgg cag cgg tcg cac gcg       697
His Ser Leu Leu Leu Val Pro Tyr Phe Ser Trp Gln Arg Ser His Ala 
         95                 100                 105    
gtg cat cac tcg agg act aat cac gtg ctc gag gga gag acg cac gtg       745
Val His His Ser Arg Thr Asn His Val Leu Glu Gly Glu Thr His Val 
    110                 115                 120     
ccg gcg cgg ttg ggg acg gag gac gcg aac gtg atg ttc aag ttg cgc       793
Pro Ala Arg Leu Gly Thr Glu Asp Ala Asn Val Met Phe Lys Leu Arg 
125                 130                 135                 140       
ggg ttg ctc ggc gaa ggg ccg ttt acg ttt ttg aac ctc gtc gga gtg       841
Gly Leu Leu Gly Glu Gly Pro Phe Thr Phe Leu Asn Leu Val Gly Val 
                145                 150                 155     
ttc gcc ttg ggg tgg ccg att tat ttg ctc acc ggc gcc agc ggt ggg       889
Phe Ala Leu Gly Trp Pro Ile Tyr Leu Leu Thr Gly Ala Ser Gly Gly 
            160                 165                 170     
ccg gtt cgc ggg aac acg aac cac ttc ttg ccg ttc atg ggc gag aag       937
Pro Val Arg Gly Asn Thr Asn His Phe Leu Pro Phe Met Gly Glu Lys 
        175                 180                 185     
ggt aag cac gcg ttg ttc ccg ggg aag tgg gcg aag aag gtt tgg cag       985
Gly Lys His Ala Leu Phe Pro Gly Lys Trp Ala Lys Lys Val Trp Gln 
    190                 195                 200     
agc gac gtc ggc gtc gtg gcg gtt ttg gga gcg ctc gcg gcg tgg gcg       1033
Ser Asp Val Gly Val Val Ala Val Leu Gly Ala Leu Ala Ala Trp Ala 
205                 210                 215                 220       
gcg cac agc ggt gtc gcc acc gtc atg gcg ctc tac gtc gga ccg tac       1081
Ala His Ser Gly Val Ala Thr Val Met Ala Leu Tyr Val Gly Pro Tyr 
                225                 230                 235     
atg gtg acc aac ttt tgg ctc gtc ctg tac acg tgg ttg cag cac act       1129
Met Val Thr Asn Phe Trp Leu Val Leu Tyr Thr Trp Leu Gln His Thr 
            240                 245                 250     
gac gtg gac gtg ccg cac ttt gag ggc gac gac tgg aac ttg gtc aag       1177
Asp Val Asp Val Pro His Phe Glu Gly Asp Asp Trp Asn Leu Val Lys 
        255                 260                 265     
ggc gcg ttt atg acg att gat cgc ccg tac ggg cca gtg ttt gac ttt       1225
Gly Ala Phe Met Thr Ile Asp Arg Pro Tyr Gly Pro Val Phe Asp Phe 
    270                 275                 280     
ttg cac cac cgc atc ggc agc acg cac gtc gcg cat cac atc aac cac       1273
Leu His His Arg Ile Gly Ser Thr His Val Ala His His Ile Asn His 
285                 290                 295                 300       
acc atc ccg cac tac cac gcc aag aag gcc acc gag gcg ttg caa aag       1321
Thr Ile Pro His Tyr His Ala Lys Lys Ala Thr Glu Ala Leu Gln Lys 
                305                 310                 315     
gcg ttc ccg gat ctg tac ctg tat gac ccg acg ccc atc gcc acg gcg       1369
Ala Phe Pro Asp Leu Tyr Leu Tyr Asp Pro Thr Pro Ile Ala Thr Ala 
            320                 325                 330     
act tgg cgc gtc ggc agc aag tgc atc gcc gtc gtc aag aag ggc gac       1417
Thr Trp Arg Val Gly Ser Lys Cys Ile Ala Val Val Lys Lys Gly Asp 
        335                 340                 345     
gag tgg gtg ttc acg gac aag cag ctc gac ccg gtc gcg gcg tga           1462
Glu Trp Val Phe Thr Asp Lys Gln Leu Asp Pro Val Ala Ala  
    350                 355                 360     
gcgcgcgtgt gacgaggata ttaagtttta gttagagaga cgagcattgt gattatgatt     1522
                                                                  

atatacgtgc gtgtttattt cacatgctgg aattcgccga agagcgaaac ggcgtattgt     1582
                                                                  

atcaccgctc gccccgcgag cgctctgctt cgtgtcgacg cgagaaaaat gtgcgctgct     1642
                                                                  

cggttttcga gcggcacaga gatcgaatga tctacagata ttaatcgcgt ttgt           1696
                                                            


<210> 2
<211> 362
<212> PRT
<213> Ostreococcus lucimarinus

<400> 2
Met Gln Glu Val Leu Arg Ala Ile Pro Arg Glu Cys Phe Glu Arg Asp 
1                 5                  10                  15     
Thr Gly Lys Ser Leu Ala Tyr Ala Ala Cys Ser Thr Ala Ile Thr Leu 
             20                  25                  30         
Ala Cys Gly Ala Leu Ala Trp Ala Cys Val Pro Val Thr Ala Ala Tyr 
         35                  40                  45             
Trp Pro Val Trp Val Ala Tyr Ala Phe Val Thr Gly Thr Ala Ala Thr 
     50                  55                  60                 
Gly Cys Trp Val Ala Ala His Glu Cys Gly His Gly Ala Phe Ser Asp 
65                   70                  75                  80 
Asp Lys Thr Ile Gln Asp Ala Val Gly Tyr Ala Leu His Ser Leu Leu 
                 85                  90                  95     
Leu Val Pro Tyr Phe Ser Trp Gln Arg Ser His Ala Val His His Ser 
            100                 105                 110         
Arg Thr Asn His Val Leu Glu Gly Glu Thr His Val Pro Ala Arg Leu 
        115                 120                 125             
Gly Thr Glu Asp Ala Asn Val Met Phe Lys Leu Arg Gly Leu Leu Gly 
    130                 135                 140                 
Glu Gly Pro Phe Thr Phe Leu Asn Leu Val Gly Val Phe Ala Leu Gly 
145                 150                 155                 160 
Trp Pro Ile Tyr Leu Leu Thr Gly Ala Ser Gly Gly Pro Val Arg Gly 
                165                 170                 175     
Asn Thr Asn His Phe Leu Pro Phe Met Gly Glu Lys Gly Lys His Ala 
            180                 185                 190         
Leu Phe Pro Gly Lys Trp Ala Lys Lys Val Trp Gln Ser Asp Val Gly 
        195                 200                 205             
Val Val Ala Val Leu Gly Ala Leu Ala Ala Trp Ala Ala His Ser Gly 
    210                 215                 220                 
Val Ala Thr Val Met Ala Leu Tyr Val Gly Pro Tyr Met Val Thr Asn 
225                 230                 235                 240 
Phe Trp Leu Val Leu Tyr Thr Trp Leu Gln His Thr Asp Val Asp Val 
                245                 250                 255     
Pro His Phe Glu Gly Asp Asp Trp Asn Leu Val Lys Gly Ala Phe Met 
            260                 265                 270         
Thr Ile Asp Arg Pro Tyr Gly Pro Val Phe Asp Phe Leu His His Arg 
        275                 280                 285             
Ile Gly Ser Thr His Val Ala His His Ile Asn His Thr Ile Pro His 
    290                 295                 300                 
Tyr His Ala Lys Lys Ala Thr Glu Ala Leu Gln Lys Ala Phe Pro Asp 
305                 310                 315                 320 
Leu Tyr Leu Tyr Asp Pro Thr Pro Ile Ala Thr Ala Thr Trp Arg Val 
                325                 330                 335     
Gly Ser Lys Cys Ile Ala Val Val Lys Lys Gly Asp Glu Trp Val Phe 
            340                 345                 350         
Thr Asp Lys Gln Leu Asp Pro Val Ala Ala 
        355                 360         

<210> 3
<211> 2039
<212> DNA
<213> Ostreococcus lucimarinus

<220>
<223> Delta-12 desaturase

<220>
<221> CDS
<222> (572)..(1900)

<400> 3
cgttctcgcc ctcgcgcgcg tcgcgaccga cgtcttctac gcttaccgac gcgcccgcgt     60
                                                                  

ccgcgaccgc gcgtctggcg cgtgctcgag cgtgccgtcg attcgccgcg ccgccgtcgc     120
                                                                  

gcgcgcgcgt cgcgccgatc ccgcggcgcg ctcgccgacg cgcgttcgcc gcgcgcgcgc     180
                                                                  

gctcgatcga ccactcgcgc gcgccgaggc gcgacgcagt gcgggcgcgc gcgccgaggc     240
                                                                  

gcggtcggtc ggtcggtcgc cgcgtgatcg cgggcgctcc gatgatggcc atccaaagcg     300
                                                                  

ctcgaatgcg tggagcgacg cgatatcagc ctgcgaatgt tcacataaca taactctgac     360
                                                                  

tgcgaatgtc cgaacacgcg gcgttcgcgg tcgcggtcct ccgcgttcgt cgcgcacacg     420
                                                                  

cgcgcgcgcg aacgacgccc gacgtcgacg cgagcgacga gcgacgcaaa gtttttacgg     480
                                                                  

cgagaatcgc gcgcgcccag gacgcacgcg cgcccaggac gtctcgacgc gacgcgcacg     540
                                                                  

acgcaaacgc ggatagcgaa cgacgaggac g atg atg acg acg gca aac gtc        592
                                   Met Met Thr Thr Ala Asn Val 
                                     1               5
tcg cgg gtc gcc gcg acg cgc gcg atg acg acg tcg acg acg tcg acg       640
Ser Arg Val Ala Ala Thr Arg Ala Met Thr Thr Ser Thr Thr Ser Thr 
         10                  15                  20  
cga cgc gtg atg gcg agt aaa tac atc gcg cga acg gcg acg acg acg       688
Arg Arg Val Met Ala Ser Lys Tyr Ile Ala Arg Thr Ala Thr Thr Thr 
     25                  30                  35  
acg acg gat gcg cgc gga cgg gcg cac cga cgc gcg ggc gcg atc gcg       736
Thr Thr Asp Ala Arg Gly Arg Ala His Arg Arg Ala Gly Ala Ile Ala 
 40                  45                  50                  55   
ctc ggt ggg ttt gaa gat ctt ccc agg agc ggg ctg gag gga cag gcg       784
Leu Gly Gly Phe Glu Asp Leu Pro Arg Ser Gly Leu Glu Gly Gln Ala 
                 60                  65                  70  
ctc acg ttc ccg cac aag aag gag ttc cct tcg cgc gcg gcg gtg ttg       832
Leu Thr Phe Pro His Lys Lys Glu Phe Pro Ser Arg Ala Ala Val Leu 
             75                  80                  85  
tca aac att ccc gat gaa tgc ttc aag aag gat acg gcc aag tcg ttg       880
Ser Asn Ile Pro Asp Glu Cys Phe Lys Lys Asp Thr Ala Lys Ser Leu 
         90                  95                 100   
atg tac gcc gcg gtg tcg acg gca atg acg gtg ggg tgc ggc ttg atc       928
Met Tyr Ala Ala Val Ser Thr Ala Met Thr Val Gly Cys Gly Leu Ile 
    105                 110                 115     
gcc gcg gcg aca ttg ccg ttg cag gcg gcg tgg tgg ccg gcg tgg ctt       976
Ala Ala Ala Thr Leu Pro Leu Gln Ala Ala Trp Trp Pro Ala Trp Leu 
120                 125                 130                 135       
gcg tac gcc gcg gtg aac ggt acg atc gcc act gga tgc tgg gtg atc       1024
Ala Tyr Ala Ala Val Asn Gly Thr Ile Ala Thr Gly Cys Trp Val Ile 
                140                 145                 150     
gcg cac gag tgc ggc cat aac gcg ttc agc gat aac cga ttc att caa       1072
Ala His Glu Cys Gly His Asn Ala Phe Ser Asp Asn Arg Phe Ile Gln 
            155                 160                 165     
gac gcg gtc ggg tac gcg ttg cac tcg gcg ctc ctc gtg ccg tac ttt       1120
Asp Ala Val Gly Tyr Ala Leu His Ser Ala Leu Leu Val Pro Tyr Phe 
        170                 175                 180     
tcc tgg cag cgg tcg cac gct gtc cac cac tca cga acg aac cac ttg       1168
Ser Trp Gln Arg Ser His Ala Val His His Ser Arg Thr Asn His Leu 
    185                 190                 195     
acg gaa gga gaa acg cac gtg ccg tac gtc aag ggc gaa ttg aag ggt       1216
Thr Glu Gly Glu Thr His Val Pro Tyr Val Lys Gly Glu Leu Lys Gly 
200                 205                 210                 215       
gac ttg aac ctc aag gcg aag aag aac ttg ggt gag ggc ccg ttc gcg       1264
Asp Leu Asn Leu Lys Ala Lys Lys Asn Leu Gly Glu Gly Pro Phe Ala 
                220                 225                 230     
att ctt cag ctc gtc acc cac ttg gtt ttc ggc tgg ccg gcg tac ttg       1312
Ile Leu Gln Leu Val Thr His Leu Val Phe Gly Trp Pro Ala Tyr Leu 
            235                 240                 245     
ctc acc ggc gcg acg ggc ggg agc gcg aga ggc gtc acc aac cat ttc       1360
Leu Thr Gly Ala Thr Gly Gly Ser Ala Arg Gly Val Thr Asn His Phe 
        250                 255                 260     
ttg cct aac atc aat acc ggc gct ttg gag ctg ttc ccg gga agc tgg       1408
Leu Pro Asn Ile Asn Thr Gly Ala Leu Glu Leu Phe Pro Gly Ser Trp 
    265                 270                 275     
aag aag aaa gtg tac tac tcg gac att ggt gtt ttc gcg ttc gtc ggc       1456
Lys Lys Lys Val Tyr Tyr Ser Asp Ile Gly Val Phe Ala Phe Val Gly 
280                 285                 290                 295       
gtt ctc gcc gcc tgg gtt gcc cag tgc ggt tgg gct ccg ttt gtc gcc       1504
Val Leu Ala Ala Trp Val Ala Gln Cys Gly Trp Ala Pro Phe Val Ala 
                300                 305                 310     
ctg tac ctg gga ccg tat ttg ttt gtc aac ttt tgg ctc gtc ttg tac       1552
Leu Tyr Leu Gly Pro Tyr Leu Phe Val Asn Phe Trp Leu Val Leu Tyr 
            315                 320                 325     
acg tgg ttg cag cac acc gat gtg gac gtc caa cac ttg gcc gcg gac       1600
Thr Trp Leu Gln His Thr Asp Val Asp Val Gln His Leu Ala Ala Asp 
        330                 335                 340     
gag tgg tcg tac atc aag ggc gca ttc ttg acg atc gac cgc ccg tac       1648
Glu Trp Ser Tyr Ile Lys Gly Ala Phe Leu Thr Ile Asp Arg Pro Tyr 
    345                 350                 355     
gga ccc gtg ttt gac ttc tta cac cac cgc atc gga tcc act cac gtg       1696
Gly Pro Val Phe Asp Phe Leu His His Arg Ile Gly Ser Thr His Val 
360                 365                 370                 375       
gcg cac cac gtt gag tgc gcg att ccg cac tac aag gcg gtc gaa gcg       1744
Ala His His Val Glu Cys Ala Ile Pro His Tyr Lys Ala Val Glu Ala 
                380                 385                 390     
acc gag gcg ttg aag acg aag tat ccg gag tac tat ttg tat gac ccg       1792
Thr Glu Ala Leu Lys Thr Lys Tyr Pro Glu Tyr Tyr Leu Tyr Asp Pro 
            395                 400                 405     
acg ccg att tgg gcg gca atg atg cgc gtc gcg tcc aag tgc gtc gct       1840
Thr Pro Ile Trp Ala Ala Met Met Arg Val Ala Ser Lys Cys Val Ala 
        410                 415                 420     
gtc gag aag cgc ggc gaa ggc aag ggt gcg atg tgg act ttc act gac       1888
Val Glu Lys Arg Gly Glu Gly Lys Gly Ala Met Trp Thr Phe Thr Asp 
    425                 430                 435     
ggc acg gcg taa ttatgaatga ttattagtat ttagcagctc ttcgcgcaca           1940
Gly Thr Ala                                              
440 
cgcagacaca aacattttat tgatacacac taacgaaact actacgtgcg attccagttg     2000
                                                                  

cgaaagacga gtacacactt gtacacacta acgaaacta                            2039
                                           


<210> 4
<211> 442
<212> PRT
<213> Ostreococcus lucimarinus

<400> 4
Met Met Thr Thr Ala Asn Val Ser Arg Val Ala Ala Thr Arg Ala Met 
1                 5                  10                  15     
Thr Thr Ser Thr Thr Ser Thr Arg Arg Val Met Ala Ser Lys Tyr Ile 
             20                  25                  30         
Ala Arg Thr Ala Thr Thr Thr Thr Thr Asp Ala Arg Gly Arg Ala His 
         35                  40                  45             
Arg Arg Ala Gly Ala Ile Ala Leu Gly Gly Phe Glu Asp Leu Pro Arg 
     50                  55                  60                 
Ser Gly Leu Glu Gly Gln Ala Leu Thr Phe Pro His Lys Lys Glu Phe 
65                   70                  75                  80 
Pro Ser Arg Ala Ala Val Leu Ser Asn Ile Pro Asp Glu Cys Phe Lys 
                 85                  90                  95     
Lys Asp Thr Ala Lys Ser Leu Met Tyr Ala Ala Val Ser Thr Ala Met 
            100                 105                 110         
Thr Val Gly Cys Gly Leu Ile Ala Ala Ala Thr Leu Pro Leu Gln Ala 
        115                 120                 125             
Ala Trp Trp Pro Ala Trp Leu Ala Tyr Ala Ala Val Asn Gly Thr Ile 
    130                 135                 140                 
Ala Thr Gly Cys Trp Val Ile Ala His Glu Cys Gly His Asn Ala Phe 
145                 150                 155                 160 
Ser Asp Asn Arg Phe Ile Gln Asp Ala Val Gly Tyr Ala Leu His Ser 
                165                 170                 175     
Ala Leu Leu Val Pro Tyr Phe Ser Trp Gln Arg Ser His Ala Val His 
            180                 185                 190         
His Ser Arg Thr Asn His Leu Thr Glu Gly Glu Thr His Val Pro Tyr 
        195                 200                 205             
Val Lys Gly Glu Leu Lys Gly Asp Leu Asn Leu Lys Ala Lys Lys Asn 
    210                 215                 220                 
Leu Gly Glu Gly Pro Phe Ala Ile Leu Gln Leu Val Thr His Leu Val 
225                 230                 235                 240 
Phe Gly Trp Pro Ala Tyr Leu Leu Thr Gly Ala Thr Gly Gly Ser Ala 
                245                 250                 255     
Arg Gly Val Thr Asn His Phe Leu Pro Asn Ile Asn Thr Gly Ala Leu 
            260                 265                 270         
Glu Leu Phe Pro Gly Ser Trp Lys Lys Lys Val Tyr Tyr Ser Asp Ile 
        275                 280                 285             
Gly Val Phe Ala Phe Val Gly Val Leu Ala Ala Trp Val Ala Gln Cys 
    290                 295                 300                 
Gly Trp Ala Pro Phe Val Ala Leu Tyr Leu Gly Pro Tyr Leu Phe Val 
305                 310                 315                 320 
Asn Phe Trp Leu Val Leu Tyr Thr Trp Leu Gln His Thr Asp Val Asp 
                325                 330                 335     
Val Gln His Leu Ala Ala Asp Glu Trp Ser Tyr Ile Lys Gly Ala Phe 
            340                 345                 350         
Leu Thr Ile Asp Arg Pro Tyr Gly Pro Val Phe Asp Phe Leu His His 
        355                 360                 365             
Arg Ile Gly Ser Thr His Val Ala His His Val Glu Cys Ala Ile Pro 
    370                 375                 380                 
His Tyr Lys Ala Val Glu Ala Thr Glu Ala Leu Lys Thr Lys Tyr Pro 
385                 390                 395                 400 
Glu Tyr Tyr Leu Tyr Asp Pro Thr Pro Ile Trp Ala Ala Met Met Arg 
                405                 410                 415     
Val Ala Ser Lys Cys Val Ala Val Glu Lys Arg Gly Glu Gly Lys Gly 
            420                 425                 430         
Ala Met Trp Thr Phe Thr Asp Gly Thr Ala 
        435                 440         

<210> 5
<211> 1737
<212> DNA
<213> Ostreococcus lucimarinus

<220>
<223> Delta-4 desaturase

<220>
<221> CDS
<222> (218)..(1618)

<400> 5
gcgcaccgtc gcgcgcgcgt tcgcgacgac gacgacgtcg agcgcgaggt gtgattatcg     60
                                                                  

ccgtcgagcg acgacgacgc gcgcgacgac gagcgcggtg cgcgcggacg cgcggcgcgc     120
                                                                  

gcgatcgggg aggtatgcga tcgttccgcg atcgatcgag ggcgaaggga acgccgcggt     180
                                                                  

cgtcgaaaga agcgtcgagg gcgaggaggg ggagaag atg atg tcc ctg gag gcg      235
                                         Met Met Ser Leu Glu Ala 
                                           1               5
aga cgc gag gcg ctg agg ttg ccg cgc ccg gac gtc gcg gcg ccg ggg       283
Arg Arg Glu Ala Leu Arg Leu Pro Arg Pro Asp Val Ala Ala Pro Gly 
             10                  15                  20  
atg gag gat ccg tgg aac gat gag aag tgg caa aag gtg aag tgg acg       331
Met Glu Asp Pro Trp Asn Asp Glu Lys Trp Gln Lys Val Lys Trp Thr 
         25                  30                  35  
gtg ttt cga gac gtg gcg tac gac ctc gga ccg ttc ttt gag aag cat       379
Val Phe Arg Asp Val Ala Tyr Asp Leu Gly Pro Phe Phe Glu Lys His 
     40                  45                  50  
ccg ggc ggg aac tgg ttg ttg aac ctc gcc atc ggt cgc gac tgc acg       427
Pro Gly Gly Asn Trp Leu Leu Asn Leu Ala Ile Gly Arg Asp Cys Thr 
 55                  60                  65                  70   
gcg ctc atg gaa tcc tat cac ttg cga cca gag gtg gcg acg gcg cgc       475
Ala Leu Met Glu Ser Tyr His Leu Arg Pro Glu Val Ala Thr Ala Arg 
                 75                  80                  85  
ttc agg atg tta ccc aag ctc gag ggg ttc ccg gtc gac gcc gtg gcg       523
Phe Arg Met Leu Pro Lys Leu Glu Gly Phe Pro Val Asp Ala Val Ala 
             90                  95                 100   
aag tca ccg aga ccg aac gat tcg ccg ctg tac aac aac atc cgc aat       571
Lys Ser Pro Arg Pro Asn Asp Ser Pro Leu Tyr Asn Asn Ile Arg Asn 
        105                 110                 115     
cgc gtt cgc gag gaa ctt ttc ccg gaa gaa ggg aag aac aaa cac aga       619
Arg Val Arg Glu Glu Leu Phe Pro Glu Glu Gly Lys Asn Lys His Arg 
    120                 125                 130     
atg ggt ggc gac cac gcg acg ata acg att ttg tct ttc gcc gcg ttt       667
Met Gly Gly Asp His Ala Thr Ile Thr Ile Leu Ser Phe Ala Ala Phe 
135                 140                 145                 150       
gcg tac ggt gtg tac gcc acc atc ccg ggc ttt ctc tcg ggt tgc ctc       715
Ala Tyr Gly Val Tyr Ala Thr Ile Pro Gly Phe Leu Ser Gly Cys Leu 
                155                 160                 165     
ctt ggc tta gct ggt gcg tgg atc ggg ctc acg att cag cat tgc gcc       763
Leu Gly Leu Ala Gly Ala Trp Ile Gly Leu Thr Ile Gln His Cys Ala 
            170                 175                 180     
aat cac ggg gcg atg tca ccg tcg ccc gcc gtc aac ggc gtc ctc ggt       811
Asn His Gly Ala Met Ser Pro Ser Pro Ala Val Asn Gly Val Leu Gly 
        185                 190                 195     
ttg acg aac gat ctc atc ggc ggc tcg tct ttg atg tgg cga tac cac       859
Leu Thr Asn Asp Leu Ile Gly Gly Ser Ser Leu Met Trp Arg Tyr His 
    200                 205                 210     
cac cag gtg agc cac cac att cac tgc aac gac aat gct atg gat caa       907
His Gln Val Ser His His Ile His Cys Asn Asp Asn Ala Met Asp Gln 
215                 220                 225                 230       
gac gtg tac acc gcc atg cct ctc ttg cgt ttc gac gct cgt cgt ccc       955
Asp Val Tyr Thr Ala Met Pro Leu Leu Arg Phe Asp Ala Arg Arg Pro 
                235                 240                 245     
aag gcg tgg tac cac cgc ttc cag cac tgg tac atg ttc ttg gcc ttt       1003
Lys Ala Trp Tyr His Arg Phe Gln His Trp Tyr Met Phe Leu Ala Phe 
            250                 255                 260     
ccg ttg ttg cag gtg gcg ttc caa gtc ggc gac atc gtc ggt ttg ttc       1051
Pro Leu Leu Gln Val Ala Phe Gln Val Gly Asp Ile Val Gly Leu Phe 
        265                 270                 275     
act cgc gat acc gaa ggc gca aag ctc cac ggg gcg acc act tgg gaa       1099
Thr Arg Asp Thr Glu Gly Ala Lys Leu His Gly Ala Thr Thr Trp Glu 
    280                 285                 290     
ctt gcc acc gta gtt atc ggc aag ttt gtg cac ttc ggt ttg ttg gct       1147
Leu Ala Thr Val Val Ile Gly Lys Phe Val His Phe Gly Leu Leu Ala 
295                 300                 305                 310       
gca cct ttg atg agc cac gcg gct tcc gcc gtc gtc gcc ggc atc gtc       1195
Ala Pro Leu Met Ser His Ala Ala Ser Ala Val Val Ala Gly Ile Val 
                315                 320                 325     
ggt ttc atg gcg tgc caa gga gtc gtc ctc gcg tgt acg ttc gcg gtg       1243
Gly Phe Met Ala Cys Gln Gly Val Val Leu Ala Cys Thr Phe Ala Val 
            330                 335                 340     
agt cac aac gtg ccc gag gcc aaa ctc cct gaa gat acc ggt gga gag       1291
Ser His Asn Val Pro Glu Ala Lys Leu Pro Glu Asp Thr Gly Gly Glu 
        345                 350                 355     
gcg tgg gaa aga gat tgg ggc gtg cag cag ttg gtg act agc gct gac       1339
Ala Trp Glu Arg Asp Trp Gly Val Gln Gln Leu Val Thr Ser Ala Asp 
    360                 365                 370     
tgg ggt gga aag att gga aac ttt ttc acc ggc ggt ctg aac ttg caa       1387
Trp Gly Gly Lys Ile Gly Asn Phe Phe Thr Gly Gly Leu Asn Leu Gln 
375                 380                 385                 390       
gtc gag cat cac ctg ttc ccg gcg att tgc ttt gtg cac tac ccc gcg       1435
Val Glu His His Leu Phe Pro Ala Ile Cys Phe Val His Tyr Pro Ala 
                395                 400                 405     
atc gcg aaa att gtc gcg gaa gaa gcg gcg aag atg ggc att cct tac       1483
Ile Ala Lys Ile Val Ala Glu Glu Ala Ala Lys Met Gly Ile Pro Tyr 
            410                 415                 420     
tcg tca tac aga aca ctt ccc ggc att ttc gtc gcg ttc tgg aaa ttc       1531
Ser Ser Tyr Arg Thr Leu Pro Gly Ile Phe Val Ala Phe Trp Lys Phe 
        425                 430                 435     
gtc aga gac atg ggc acg gcg gag caa atc gac gac gtt tta ctt ccg       1579
Val Arg Asp Met Gly Thr Ala Glu Gln Ile Asp Asp Val Leu Leu Pro 
    440                 445                 450     
aag ttc gcg aac ccg caa ctt tcg cca gcc atc aat tag aattataact        1628
Lys Phe Ala Asn Pro Gln Leu Ser Pro Ala Ile Asn             
455                 460                 465     
taccttacgc gcttgttgac ttcaaacgag caaacgacag aaatacgatt attgtgatat     1688
                                                                  

acttacacct ccgcctcatc attgttttga tctaattcta acttacaaa                 1737
                                                      


<210> 6
<211> 466
<212> PRT
<213> Ostreococcus lucimarinus

<400> 6
Met Met Ser Leu Glu Ala Arg Arg Glu Ala Leu Arg Leu Pro Arg Pro 
1                 5                  10                  15     
Asp Val Ala Ala Pro Gly Met Glu Asp Pro Trp Asn Asp Glu Lys Trp 
             20                  25                  30         
Gln Lys Val Lys Trp Thr Val Phe Arg Asp Val Ala Tyr Asp Leu Gly 
         35                  40                  45             
Pro Phe Phe Glu Lys His Pro Gly Gly Asn Trp Leu Leu Asn Leu Ala 
     50                  55                  60                 
Ile Gly Arg Asp Cys Thr Ala Leu Met Glu Ser Tyr His Leu Arg Pro 
65                   70                  75                  80 
Glu Val Ala Thr Ala Arg Phe Arg Met Leu Pro Lys Leu Glu Gly Phe 
                 85                  90                  95     
Pro Val Asp Ala Val Ala Lys Ser Pro Arg Pro Asn Asp Ser Pro Leu 
            100                 105                 110         
Tyr Asn Asn Ile Arg Asn Arg Val Arg Glu Glu Leu Phe Pro Glu Glu 
        115                 120                 125             
Gly Lys Asn Lys His Arg Met Gly Gly Asp His Ala Thr Ile Thr Ile 
    130                 135                 140                 
Leu Ser Phe Ala Ala Phe Ala Tyr Gly Val Tyr Ala Thr Ile Pro Gly 
145                 150                 155                 160 
Phe Leu Ser Gly Cys Leu Leu Gly Leu Ala Gly Ala Trp Ile Gly Leu 
                165                 170                 175     
Thr Ile Gln His Cys Ala Asn His Gly Ala Met Ser Pro Ser Pro Ala 
            180                 185                 190         
Val Asn Gly Val Leu Gly Leu Thr Asn Asp Leu Ile Gly Gly Ser Ser 
        195                 200                 205             
Leu Met Trp Arg Tyr His His Gln Val Ser His His Ile His Cys Asn 
    210                 215                 220                 
Asp Asn Ala Met Asp Gln Asp Val Tyr Thr Ala Met Pro Leu Leu Arg 
225                 230                 235                 240 
Phe Asp Ala Arg Arg Pro Lys Ala Trp Tyr His Arg Phe Gln His Trp 
                245                 250                 255     
Tyr Met Phe Leu Ala Phe Pro Leu Leu Gln Val Ala Phe Gln Val Gly 
            260                 265                 270         
Asp Ile Val Gly Leu Phe Thr Arg Asp Thr Glu Gly Ala Lys Leu His 
        275                 280                 285             
Gly Ala Thr Thr Trp Glu Leu Ala Thr Val Val Ile Gly Lys Phe Val 
    290                 295                 300                 
His Phe Gly Leu Leu Ala Ala Pro Leu Met Ser His Ala Ala Ser Ala 
305                 310                 315                 320 
Val Val Ala Gly Ile Val Gly Phe Met Ala Cys Gln Gly Val Val Leu 
                325                 330                 335     
Ala Cys Thr Phe Ala Val Ser His Asn Val Pro Glu Ala Lys Leu Pro 
            340                 345                 350         
Glu Asp Thr Gly Gly Glu Ala Trp Glu Arg Asp Trp Gly Val Gln Gln 
        355                 360                 365             
Leu Val Thr Ser Ala Asp Trp Gly Gly Lys Ile Gly Asn Phe Phe Thr 
    370                 375                 380                 
Gly Gly Leu Asn Leu Gln Val Glu His His Leu Phe Pro Ala Ile Cys 
385                 390                 395                 400 
Phe Val His Tyr Pro Ala Ile Ala Lys Ile Val Ala Glu Glu Ala Ala 
                405                 410                 415     
Lys Met Gly Ile Pro Tyr Ser Ser Tyr Arg Thr Leu Pro Gly Ile Phe 
            420                 425                 430         
Val Ala Phe Trp Lys Phe Val Arg Asp Met Gly Thr Ala Glu Gln Ile 
        435                 440                 445             
Asp Asp Val Leu Leu Pro Lys Phe Ala Asn Pro Gln Leu Ser Pro Ala 
    450                 455                 460                 
Ile Asn 
465     


<210> 7
<211> 1604
<212> DNA
<213> Ostreococcus lucimarinus

<220>
<223> Delta-5 desaturase

<220>
<221> CDS
<222> (156)..(1535)

<400> 7
tcactcagcc gtccgcgatc cgcgcgcgcg tcgcgttcga ccgcgtcgcg cccagaacga     60
                                                                  

acgtagaacc gaatcgcccg tcccgcgtcg cgcggatgaa tgcaaaaaac aagatgactg     120
                                                                  

gatgacgtcc cgcgccacac gatcgcgcgc gcgtc atg ccc tcc gcc gcg cgc        173
                                       Met Pro Ser Ala Ala Arg 
                                         1               5
tcc cga gcg tcg aag cgc gcg aac gcg acg acg gac gtc gcg acg acc       221
Ser Arg Ala Ser Lys Arg Ala Asn Ala Thr Thr Asp Val Ala Thr Thr 
             10                  15                  20  
gct ccc gag gcg acg ctc gac ccg acg cgc gcg tac acg cgc tat cgc       269
Ala Pro Glu Ala Thr Leu Asp Pro Thr Arg Ala Tyr Thr Arg Tyr Arg 
         25                  30                  35  
ggc gtc gtc tac gac gtc acc gag ttt cag cat cgc cat ccc ggc ggc       317
Gly Val Val Tyr Asp Val Thr Glu Phe Gln His Arg His Pro Gly Gly 
     40                  45                  50  
gcg caa ttg ctc tcg ctc tgc gtc gga cgc gac gcg acg atc ctg atc       365
Ala Gln Leu Leu Ser Leu Cys Val Gly Arg Asp Ala Thr Ile Leu Ile 
 55                  60                  65                  70   
gag agc cat cac ttg cga cct gag gtg gtg cgc aag tac atg aag acg       413
Glu Ser His His Leu Arg Pro Glu Val Val Arg Lys Tyr Met Lys Thr 
                 75                  80                  85  
ctg ccc gtg gtg gag ggc gcg gcg ggc gcg ttc ggg aag gag gag acg       461
Leu Pro Val Val Glu Gly Ala Ala Gly Ala Phe Gly Lys Glu Glu Thr 
             90                  95                 100   
ttt ccg aaa ccg ctc gat tcg gcg gtg tat cga gcg ata caa cgc cga       509
Phe Pro Lys Pro Leu Asp Ser Ala Val Tyr Arg Ala Ile Gln Arg Arg 
        105                 110                 115     
gtg cga gat gaa gtc gtg gaa ccg atg aaa gca aag tct gga agg gag       557
Val Arg Asp Glu Val Val Glu Pro Met Lys Ala Lys Ser Gly Arg Glu 
    120                 125                 130     
gca cac gga cgc ggt ggg tgc gtc gtg gac gcg ggg gtg gtc gtg ttg       605
Ala His Gly Arg Gly Gly Cys Val Val Asp Ala Gly Val Val Val Leu 
135                 140                 145                 150       
acg ttt gtc gcg gcg atg gtg gcg tat tgg cgc gcg ccc tcg gcg ctc       653
Thr Phe Val Ala Ala Met Val Ala Tyr Trp Arg Ala Pro Ser Ala Leu 
                155                 160                 165     
acg ggg tgc gcg ttg gga ctg gcg ggg tac tgg agc ggc act ggg ttg       701
Thr Gly Cys Ala Leu Gly Leu Ala Gly Tyr Trp Ser Gly Thr Gly Leu 
            170                 175                 180     
caa cac acg gcg aat cac gga gga ttg gcg aag agt gga ttt tgg aat       749
Gln His Thr Ala Asn His Gly Gly Leu Ala Lys Ser Gly Phe Trp Asn 
        185                 190                 195     
cag ttt tgg gga tgg ctc ggg aac gac gtg gcg atc ggg aag agt tcg       797
Gln Phe Trp Gly Trp Leu Gly Asn Asp Val Ala Ile Gly Lys Ser Ser 
    200                 205                 210     
gtg gag tgg cgg tat cat cac atg gtg agc cat cac tcg tac tgc aac       845
Val Glu Trp Arg Tyr His His Met Val Ser His His Ser Tyr Cys Asn 
215                 220                 225                 230       
gac gcc gat ctc gat caa gac gtg tac acc gcg tta ccg ttg ttg cga       893
Asp Ala Asp Leu Asp Gln Asp Val Tyr Thr Ala Leu Pro Leu Leu Arg 
                235                 240                 245     
ctt gac cca tcg cag gag ttg aag tgg ttc cat cgg tac caa gcg ttt       941
Leu Asp Pro Ser Gln Glu Leu Lys Trp Phe His Arg Tyr Gln Ala Phe 
            250                 255                 260     
tac gcg ccg ctc atg tgg ccg ttt tta tgg ctc gcc gcg caa gtc ggt       989
Tyr Ala Pro Leu Met Trp Pro Phe Leu Trp Leu Ala Ala Gln Val Gly 
        265                 270                 275     
gac gcg caa aac att tta atc gat cga gcg tct ccc ggc gtc gag tac       1037
Asp Ala Gln Asn Ile Leu Ile Asp Arg Ala Ser Pro Gly Val Glu Tyr 
    280                 285                 290     
aag ggc ttg atg aag aac gaa atc gcg ctg tac ctg ctc ggt aaa gtt       1085
Lys Gly Leu Met Lys Asn Glu Ile Ala Leu Tyr Leu Leu Gly Lys Val 
295                 300                 305                 310       
ctg cac ttc ggc tta ctt ctc ggc gtt ccg gcg tac atc cac ggg ttg       1133
Leu His Phe Gly Leu Leu Leu Gly Val Pro Ala Tyr Ile His Gly Leu 
                315                 320                 325     
tcc aac gtc atc gtg ccg ttc ctc gcg tac ggc gcg ttt ggc tcg ttc       1181
Ser Asn Val Ile Val Pro Phe Leu Ala Tyr Gly Ala Phe Gly Ser Phe 
            330                 335                 340     
gtc ctt tgc tgg ttc ttc atc gtc agc cac aac ttg gag gcg ttg acc       1229
Val Leu Cys Trp Phe Phe Ile Val Ser His Asn Leu Glu Ala Leu Thr 
        345                 350                 355     
ccg atg aac ttg agc aag agc act aag aac gat tgg ggt gcg tgg caa       1277
Pro Met Asn Leu Ser Lys Ser Thr Lys Asn Asp Trp Gly Ala Trp Gln 
    360                 365                 370     
atc gaa acg tcc gcg tcg tgg ggt aac agc ttc tgg agc ttc ttt tcc       1325
Ile Glu Thr Ser Ala Ser Trp Gly Asn Ser Phe Trp Ser Phe Phe Ser 
375                 380                 385                 390       
ggt ggt ttg aac ttg caa atc gag cac cac ttg ttt ccg gga tgc gcg       1373
Gly Gly Leu Asn Leu Gln Ile Glu His His Leu Phe Pro Gly Cys Ala 
                395                 400                 405     
cac aat ctg tac ccg aag atg gtt ccc atc atc aag gat gag tgc aaa       1421
His Asn Leu Tyr Pro Lys Met Val Pro Ile Ile Lys Asp Glu Cys Lys 
            410                 415                 420     
aag gct ggc ctc gcg tac acc ggc tat ggt ggc tac ttt gga ttg ttg       1469
Lys Ala Gly Leu Ala Tyr Thr Gly Tyr Gly Gly Tyr Phe Gly Leu Leu 
        425                 430                 435     
ccc atc acg cgc gac atg ttt tcc tat tta cac aag atg ggc cat caa       1517
Pro Ile Thr Arg Asp Met Phe Ser Tyr Leu His Lys Met Gly His Gln 
    440                 445                 450     
cga ccg aag gcc atg tag aaatccgaac aaaccgttgt acctcttgtt              1565
Arg Pro Lys Ala Met                                   
455 
tgatgaatac agtatttatt ttacaacgca actacgcat                            1604
                                           


<210> 8
<211> 459
<212> PRT
<213> Ostreococcus lucimarinus

<400> 8
Met Pro Ser Ala Ala Arg Ser Arg Ala Ser Lys Arg Ala Asn Ala Thr 
1                 5                  10                  15     
Thr Asp Val Ala Thr Thr Ala Pro Glu Ala Thr Leu Asp Pro Thr Arg 
             20                  25                  30         
Ala Tyr Thr Arg Tyr Arg Gly Val Val Tyr Asp Val Thr Glu Phe Gln 
         35                  40                  45             
His Arg His Pro Gly Gly Ala Gln Leu Leu Ser Leu Cys Val Gly Arg 
     50                  55                  60                 
Asp Ala Thr Ile Leu Ile Glu Ser His His Leu Arg Pro Glu Val Val 
65                   70                  75                  80 
Arg Lys Tyr Met Lys Thr Leu Pro Val Val Glu Gly Ala Ala Gly Ala 
                 85                  90                  95     
Phe Gly Lys Glu Glu Thr Phe Pro Lys Pro Leu Asp Ser Ala Val Tyr 
            100                 105                 110         
Arg Ala Ile Gln Arg Arg Val Arg Asp Glu Val Val Glu Pro Met Lys 
        115                 120                 125             
Ala Lys Ser Gly Arg Glu Ala His Gly Arg Gly Gly Cys Val Val Asp 
    130                 135                 140                 
Ala Gly Val Val Val Leu Thr Phe Val Ala Ala Met Val Ala Tyr Trp 
145                 150                 155                 160 
Arg Ala Pro Ser Ala Leu Thr Gly Cys Ala Leu Gly Leu Ala Gly Tyr 
                165                 170                 175     
Trp Ser Gly Thr Gly Leu Gln His Thr Ala Asn His Gly Gly Leu Ala 
            180                 185                 190         
Lys Ser Gly Phe Trp Asn Gln Phe Trp Gly Trp Leu Gly Asn Asp Val 
        195                 200                 205             
Ala Ile Gly Lys Ser Ser Val Glu Trp Arg Tyr His His Met Val Ser 
    210                 215                 220                 
His His Ser Tyr Cys Asn Asp Ala Asp Leu Asp Gln Asp Val Tyr Thr 
225                 230                 235                 240 
Ala Leu Pro Leu Leu Arg Leu Asp Pro Ser Gln Glu Leu Lys Trp Phe 
                245                 250                 255     
His Arg Tyr Gln Ala Phe Tyr Ala Pro Leu Met Trp Pro Phe Leu Trp 
            260                 265                 270         
Leu Ala Ala Gln Val Gly Asp Ala Gln Asn Ile Leu Ile Asp Arg Ala 
        275                 280                 285             
Ser Pro Gly Val Glu Tyr Lys Gly Leu Met Lys Asn Glu Ile Ala Leu 
    290                 295                 300                 
Tyr Leu Leu Gly Lys Val Leu His Phe Gly Leu Leu Leu Gly Val Pro 
305                 310                 315                 320 
Ala Tyr Ile His Gly Leu Ser Asn Val Ile Val Pro Phe Leu Ala Tyr 
                325                 330                 335     
Gly Ala Phe Gly Ser Phe Val Leu Cys Trp Phe Phe Ile Val Ser His 
            340                 345                 350         
Asn Leu Glu Ala Leu Thr Pro Met Asn Leu Ser Lys Ser Thr Lys Asn 
        355                 360                 365             
Asp Trp Gly Ala Trp Gln Ile Glu Thr Ser Ala Ser Trp Gly Asn Ser 
    370                 375                 380                 
Phe Trp Ser Phe Phe Ser Gly Gly Leu Asn Leu Gln Ile Glu His His 
385                 390                 395                 400 
Leu Phe Pro Gly Cys Ala His Asn Leu Tyr Pro Lys Met Val Pro Ile 
                405                 410                 415     
Ile Lys Asp Glu Cys Lys Lys Ala Gly Leu Ala Tyr Thr Gly Tyr Gly 
            420                 425                 430         
Gly Tyr Phe Gly Leu Leu Pro Ile Thr Arg Asp Met Phe Ser Tyr Leu 
        435                 440                 445             
His Lys Met Gly His Gln Arg Pro Lys Ala Met 
    450                 455                 

<210> 9
<211> 2297
<212> DNA
<213> Ostreococcus lucimarinus

<220>
<223> Delta-5 desaturase

<220>
<221> CDS
<222> (629)..(2104)

<400> 9
cgcgcgcgcg tcgccgcgac ccccctcgaa gtcgcgtacc gttcgcccgc gtcgctcgcg     60
                                                                  

tcgcgctctc gccgtctcgc gcgcgcgtcg cgtcgcgatg cgtcttcgcg cgtcgtcgcg     120
                                                                  

atcggcgtct tccggcgtcc acgattcgcg cgcgcgcgtc ctcctccgcg gcgctcgcgc     180
                                                                  

gcgcgcgtcg atcgcgtcgc gtcgatcgcg gtctctctcg cggttcctcg cggttcgcgc     240
                                                                  

ctccctcgcg cgcgcgtcga gcgcgcgccg tcgtctgcgg cgccgtcgcc gtttcccgcg     300
                                                                  

cgattcgtcc gacacgcgtc cgacacgcgt gcgacacgcg cgtttacata ccacaaatac     360
                                                                  

cacacagtgt acgaccaaca ttaccgtact gtagaagaaa tgatggtcac tcgatgtata     420
                                                                  

cagaccccgt cactgatccc catacgcgac cgaccgcccc gcccggtgac gtcggcgcgc     480
                                                                  

gcgcgcgtcg ccacacgacg ccgcgcgctc gccgcgcgcg ctcgactcgc gcgccgcgtc     540
                                                                  

gcgcgccgtc gcgctcaccg cgcgcccgcg accgccggcg tcgcgcgtcg acgacgccgc     600
                                                                  

gcggcgaacg agggcgcgac gcgacgcg atg acg acg gtc gcg gag atc gtc        652
                               Met Thr Thr Val Ala Glu Ile Val 
                                 1               5
gac gac gac gcg cgg cgc gcg gga aag ggc gcg acg acg cga cga cga       700
Asp Asp Asp Ala Arg Arg Ala Gly Lys Gly Ala Thr Thr Arg Arg Arg 
     10                  15                  20  
cgc gcg acg acg cgc gtc gcg cgc gat gac gcg cgc gag gcg acg tac       748
Arg Ala Thr Thr Arg Val Ala Arg Asp Asp Ala Arg Glu Ala Thr Tyr 
 25                  30                  35                  40   
acg gcg gcg gag gtg gcg agg cac gcg cgc gcg gac gac tgc tgg gtg       796
Thr Ala Ala Glu Val Ala Arg His Ala Arg Ala Asp Asp Cys Trp Val 
                 45                  50                  55  
atc gtg cga ggc ggc gtg tac gac gtg acg agg ttc gtg ccg aga cac       844
Ile Val Arg Gly Gly Val Tyr Asp Val Thr Arg Phe Val Pro Arg His 
             60                  65                  70  
ccg gga ggg aac atg ata tac gtc aag gcg ggg ggc gag tgc acg gcg       892
Pro Gly Gly Asn Met Ile Tyr Val Lys Ala Gly Gly Glu Cys Thr Ala 
         75                  80                  85  
ctg ttc gac tcg tat cat ccc gag cgc gcg agg gcg acg ctg gag aag       940
Leu Phe Asp Ser Tyr His Pro Glu Arg Ala Arg Ala Thr Leu Glu Lys 
     90                  95                 100   
tat agg atc ggc gcg ctg cgg agg gac gcg ggg gaa cgc gag gac gag       988
Tyr Arg Ile Gly Ala Leu Arg Arg Asp Ala Gly Glu Arg Glu Asp Glu 
105                 110                 115                 120       
gac gtg gtg gag tac ttg aag gac gat ctg agg gag gga gag ttt tac       1036
Asp Val Val Glu Tyr Leu Lys Asp Asp Leu Arg Glu Gly Glu Phe Tyr 
                125                 130                 135     
gcg gat tgt aag gcg ggg gcg gcg aag tat ttt aag gat aat aag ctc       1084
Ala Asp Cys Lys Ala Gly Ala Ala Lys Tyr Phe Lys Asp Asn Lys Leu 
            140                 145                 150     
gat ccg cgc gtg cac tgg gag atg tac gcg aag acg ttg gtg att ttg       1132
Asp Pro Arg Val His Trp Glu Met Tyr Ala Lys Thr Leu Val Ile Leu 
        155                 160                 165     
acg ggc gtc gtc gtc gga cac tac ggg tcg ttt ttc gcg ccg tcg gcg       1180
Thr Gly Val Val Val Gly His Tyr Gly Ser Phe Phe Ala Pro Ser Ala 
    170                 175                 180     
tcc ttc gcc gcg gcg ttg gcg ctc gcg gtg ctg cac ggc acg tgc aag       1228
Ser Phe Ala Ala Ala Leu Ala Leu Ala Val Leu His Gly Thr Cys Lys 
185                 190                 195                 200       
gcg gaa gta gga gtg tcg atc cag cac gac gcc aat cac ggg gcg tac       1276
Ala Glu Val Gly Val Ser Ile Gln His Asp Ala Asn His Gly Ala Tyr 
                205                 210                 215     
gga aac aat cgc act tgg ttg cac gcg atg cag ttg acc ttg gac gcc       1324
Gly Asn Asn Arg Thr Trp Leu His Ala Met Gln Leu Thr Leu Asp Ala 
            220                 225                 230     
gtc ggc gcg tcg agc ttc atg tgg aag cag cag cac gtc gcc gga cat       1372
Val Gly Ala Ser Ser Phe Met Trp Lys Gln Gln His Val Ala Gly His 
        235                 240                 245     
cac gcg tac acc aac gtc gaa ggc atc gat ccc gac att cga tgc tcg       1420
His Ala Tyr Thr Asn Val Glu Gly Ile Asp Pro Asp Ile Arg Cys Ser 
    250                 255                 260     
gaa aag gac gtg cga cgg gtg aac gag cat cag cct cac gag ccg tac       1468
Glu Lys Asp Val Arg Arg Val Asn Glu His Gln Pro His Glu Pro Tyr 
265                 270                 275                 280       
cac aga gtt caa cac gtc tac ttg gcg ttg atg tac ggc ttg ttg tcg       1516
His Arg Val Gln His Val Tyr Leu Ala Leu Met Tyr Gly Leu Leu Ser 
                285                 290                 295     
ttc aaa tcg tgc ttc gtg gac gac ttc aac gcc ttc ttc tcc ggc cga       1564
Phe Lys Ser Cys Phe Val Asp Asp Phe Asn Ala Phe Phe Ser Gly Arg 
            300                 305                 310     
atc ggt tgg gtg aag gtg atg aag ttt act cgc ggt gaa gcc gtg gcg       1612
Ile Gly Trp Val Lys Val Met Lys Phe Thr Arg Gly Glu Ala Val Ala 
        315                 320                 325     
ttt tgg gga agc aag ctc gcg tgg gcg ttt tac tac ctc tac ttg ccc       1660
Phe Trp Gly Ser Lys Leu Ala Trp Ala Phe Tyr Tyr Leu Tyr Leu Pro 
    330                 335                 340     
gcc aag tac tcc cat cgc tcg atc ggt cag ctc ttg gcg ctc tgg acc       1708
Ala Lys Tyr Ser His Arg Ser Ile Gly Gln Leu Leu Ala Leu Trp Thr 
345                 350                 355                 360       
gtc acc gag ttc gtc acc ggt tgg ttg ctg gcg ttc atg ttc caa gtc       1756
Val Thr Glu Phe Val Thr Gly Trp Leu Leu Ala Phe Met Phe Gln Val 
                365                 370                 375     
gcc cac gtc gtc ggc gac gtc cac ttc ttc cgc ctc aac gaa aag aac       1804
Ala His Val Val Gly Asp Val His Phe Phe Arg Leu Asn Glu Lys Asn 
            380                 385                 390     
cag ctc aac aag ggc tgg ggc gag gcg caa ctc atg act tcg gct gat       1852
Gln Leu Asn Lys Gly Trp Gly Glu Ala Gln Leu Met Thr Ser Ala Asp 
        395                 400                 405     
ttc gcg cac ggc agc aag ttt tgg acc cac ttc tcc ggc ggc tta aac       1900
Phe Ala His Gly Ser Lys Phe Trp Thr His Phe Ser Gly Gly Leu Asn 
    410                 415                 420     
tat caa gtc gtc cac cac ctc ttc ccg ggc gtg tgt cac gtg cac tac       1948
Tyr Gln Val Val His His Leu Phe Pro Gly Val Cys His Val His Tyr 
425                 430                 435                 440       
ccc gcg ctc gcg ccg atc atc aag gct gcc gcg gat aag cac gga tta       1996
Pro Ala Leu Ala Pro Ile Ile Lys Ala Ala Ala Asp Lys His Gly Leu 
                445                 450                 455     
cac tat caa atc tac ccc acg ttt tgg tcc gcg ctg cgc gcg cac ttc       2044
His Tyr Gln Ile Tyr Pro Thr Phe Trp Ser Ala Leu Arg Ala His Phe 
            460                 465                 470     
tcc cac ctc gcc cgc gtc ggc cac gag gcg tac gtg cct tcc ctc cga       2092
Ser His Leu Ala Arg Val Gly His Glu Ala Tyr Val Pro Ser Leu Arg 
        475                 480                 485     
acc gtc ggg tga gcgcgcctcg cgcgcggcca tttattcata cctttacctc           2144
Thr Val Gly                                              
    490 
accccgacgc gtcgatcgct cgccggcgct cccatcgcat ttattttaat ctccacgtct     2204
                                                                  

tccgtccatc cctcgcgcct tcgcctcgcg ctcgcgctcg cctcgcctcg cccgggcttc     2264
                                                                  

ctccccactc cgccgcgcgc cgtcgcgcgc gcg                                  2297
                                     


<210> 10
<211> 491
<212> PRT
<213> Ostreococcus lucimarinus

<400> 10
Met Thr Thr Val Ala Glu Ile Val Asp Asp Asp Ala Arg Arg Ala Gly 
1                 5                  10                  15     
Lys Gly Ala Thr Thr Arg Arg Arg Arg Ala Thr Thr Arg Val Ala Arg 
             20                  25                  30         
Asp Asp Ala Arg Glu Ala Thr Tyr Thr Ala Ala Glu Val Ala Arg His 
         35                  40                  45             
Ala Arg Ala Asp Asp Cys Trp Val Ile Val Arg Gly Gly Val Tyr Asp 
     50                  55                  60                 
Val Thr Arg Phe Val Pro Arg His Pro Gly Gly Asn Met Ile Tyr Val 
65                   70                  75                  80 
Lys Ala Gly Gly Glu Cys Thr Ala Leu Phe Asp Ser Tyr His Pro Glu 
                 85                  90                  95     
Arg Ala Arg Ala Thr Leu Glu Lys Tyr Arg Ile Gly Ala Leu Arg Arg 
            100                 105                 110         
Asp Ala Gly Glu Arg Glu Asp Glu Asp Val Val Glu Tyr Leu Lys Asp 
        115                 120                 125             
Asp Leu Arg Glu Gly Glu Phe Tyr Ala Asp Cys Lys Ala Gly Ala Ala 
    130                 135                 140                 
Lys Tyr Phe Lys Asp Asn Lys Leu Asp Pro Arg Val His Trp Glu Met 
145                 150                 155                 160 
Tyr Ala Lys Thr Leu Val Ile Leu Thr Gly Val Val Val Gly His Tyr 
                165                 170                 175     
Gly Ser Phe Phe Ala Pro Ser Ala Ser Phe Ala Ala Ala Leu Ala Leu 
            180                 185                 190         
Ala Val Leu His Gly Thr Cys Lys Ala Glu Val Gly Val Ser Ile Gln 
        195                 200                 205             
His Asp Ala Asn His Gly Ala Tyr Gly Asn Asn Arg Thr Trp Leu His 
    210                 215                 220                 
Ala Met Gln Leu Thr Leu Asp Ala Val Gly Ala Ser Ser Phe Met Trp 
225                 230                 235                 240 
Lys Gln Gln His Val Ala Gly His His Ala Tyr Thr Asn Val Glu Gly 
                245                 250                 255     
Ile Asp Pro Asp Ile Arg Cys Ser Glu Lys Asp Val Arg Arg Val Asn 
            260                 265                 270         
Glu His Gln Pro His Glu Pro Tyr His Arg Val Gln His Val Tyr Leu 
        275                 280                 285             
Ala Leu Met Tyr Gly Leu Leu Ser Phe Lys Ser Cys Phe Val Asp Asp 
    290                 295                 300                 
Phe Asn Ala Phe Phe Ser Gly Arg Ile Gly Trp Val Lys Val Met Lys 
305                 310                 315                 320 
Phe Thr Arg Gly Glu Ala Val Ala Phe Trp Gly Ser Lys Leu Ala Trp 
                325                 330                 335     
Ala Phe Tyr Tyr Leu Tyr Leu Pro Ala Lys Tyr Ser His Arg Ser Ile 
            340                 345                 350         
Gly Gln Leu Leu Ala Leu Trp Thr Val Thr Glu Phe Val Thr Gly Trp 
        355                 360                 365             
Leu Leu Ala Phe Met Phe Gln Val Ala His Val Val Gly Asp Val His 
    370                 375                 380                 
Phe Phe Arg Leu Asn Glu Lys Asn Gln Leu Asn Lys Gly Trp Gly Glu 
385                 390                 395                 400 
Ala Gln Leu Met Thr Ser Ala Asp Phe Ala His Gly Ser Lys Phe Trp 
                405                 410                 415     
Thr His Phe Ser Gly Gly Leu Asn Tyr Gln Val Val His His Leu Phe 
            420                 425                 430         
Pro Gly Val Cys His Val His Tyr Pro Ala Leu Ala Pro Ile Ile Lys 
        435                 440                 445             
Ala Ala Ala Asp Lys His Gly Leu His Tyr Gln Ile Tyr Pro Thr Phe 
    450                 455                 460                 
Trp Ser Ala Leu Arg Ala His Phe Ser His Leu Ala Arg Val Gly His 
465                 470                 475                 480 
Glu Ala Tyr Val Pro Ser Leu Arg Thr Val Gly 
                485                 490     

<210> 11
<211> 1412
<212> DNA
<213> Ostreococcus lucimarinus

<220>
<223> Delta-5 elongase

<220>
<221> CDS
<222> (254)..(1150)

<400> 11
cgcgcgcgca ctttttcgcc caccgcgcgc cgtcgcgcac tgcgccgtcg tcgcgcgtcg     60
                                                                  

cgcgaacgcc gacatgtcaa acgttacgtt cgccgccgtc tcgcgcgcgt cgctccgtct     120
                                                                  

ctctcgcgcg cgtcgtgcgt cgagcgccgc gaaatgtcgc cgagcgtgcg ctatccgttc     180
                                                                  

gccaccgcga catcgtcgcc actgacgcgc gcgtcgcccg tcgctcgtcg ctcgcgcgcg     240
                                                                  

cgcacgcgga gac atg gcg cag ttt ccg ctc gtt tcg ctg tgt gct ttc        289
               Met Ala Gln Phe Pro Leu Val Ser Leu Cys Ala Phe 
                 1               5                  10
gcg gtg tac ggc tac gcg acg tac gcg tac gcg ttc gaa tgg tcg cac       337
Ala Val Tyr Gly Tyr Ala Thr Tyr Ala Tyr Ala Phe Glu Trp Ser His 
         15                  20                  25  
gcg cgc acg ccc gga ggt ttg gcg aac gtc gac gcg cag cga tgg atc       385
Ala Arg Thr Pro Gly Gly Leu Ala Asn Val Asp Ala Gln Arg Trp Ile 
     30                  35                  40  
ggt gat ctc tcg ttc gcg ctc ccg gcg tgc gcg acg acg gcg tac ttg       433
Gly Asp Leu Ser Phe Ala Leu Pro Ala Cys Ala Thr Thr Ala Tyr Leu 
 45                  50                  55                  60   
atg ttt tgc ctc gtc ggg ccg cgc gtg atg gcg aag cga gag gcg ttc       481
Met Phe Cys Leu Val Gly Pro Arg Val Met Ala Lys Arg Glu Ala Phe 
                 65                  70                  75  
gat ccg aaa ggg ctc atg ctg gcg tac aac gcg tat cag acg gcg ttc       529
Asp Pro Lys Gly Leu Met Leu Ala Tyr Asn Ala Tyr Gln Thr Ala Phe 
             80                  85                  90  
aac gtg tgc gtg ctc ggg atg ttt att cgg gag atc gtg acg ctg aaa       577
Asn Val Cys Val Leu Gly Met Phe Ile Arg Glu Ile Val Thr Leu Lys 
         95                 100                 105    
caa ccg acg tgg ggg tcg aag atg ccg tgg agc gat aaa cgg tcg ttt       625
Gln Pro Thr Trp Gly Ser Lys Met Pro Trp Ser Asp Lys Arg Ser Phe 
    110                 115                 120     
aac atc ttg ctc ggg gtg tgg ttt cat tac aac aac aag tat ttg gaa       673
Asn Ile Leu Leu Gly Val Trp Phe His Tyr Asn Asn Lys Tyr Leu Glu 
125                 130                 135                 140       
ttg ctg gac acg gcg ttt atg att gcc cgc aag aag acg aat caa ctg       721
Leu Leu Asp Thr Ala Phe Met Ile Ala Arg Lys Lys Thr Asn Gln Leu 
                145                 150                 155     
agc ttt ttg cac gtg tat cat cac gca ctg ttg att tgg gca tgg tgg       769
Ser Phe Leu His Val Tyr His His Ala Leu Leu Ile Trp Ala Trp Trp 
            160                 165                 170     
ttc gtg tgt cat ctg atg gcg acg aac gat tgc gtc gac gcg tac ttt       817
Phe Val Cys His Leu Met Ala Thr Asn Asp Cys Val Asp Ala Tyr Phe 
        175                 180                 185     
ggt gcg gcg tgt aac tcg ttc att cac atc gtg atg tac tcg tac tat       865
Gly Ala Ala Cys Asn Ser Phe Ile His Ile Val Met Tyr Ser Tyr Tyr 
    190                 195                 200     
tta atg gcg gcg ctc ggc gtg cga tgc ccc tgg aag cgt tac atc acg       913
Leu Met Ala Ala Leu Gly Val Arg Cys Pro Trp Lys Arg Tyr Ile Thr 
205                 210                 215                 220       
cag gcg caa atg tta caa ttt gtc atc gtt ttc gtc cac gcc gtg ttc       961
Gln Ala Gln Met Leu Gln Phe Val Ile Val Phe Val His Ala Val Phe 
                225                 230                 235     
gtc tta cga gaa aag cac tgc cca gtc tca tta cca tgg gcg caa atg       1009
Val Leu Arg Glu Lys His Cys Pro Val Ser Leu Pro Trp Ala Gln Met 
            240                 245                 250     
ttc gtc atg gcg aac atg ctc gtg tta ttc ggt aac ttc tac ctc aag       1057
Phe Val Met Ala Asn Met Leu Val Leu Phe Gly Asn Phe Tyr Leu Lys 
        255                 260                 265     
gcg tac gcc gcc aaa ccg tcg ggc aaa tcc tcg acg cgc gtc tcc gcc       1105
Ala Tyr Ala Ala Lys Pro Ser Gly Lys Ser Ser Thr Arg Val Ser Ala 
    270                 275                 280     
gcc aaa ccg gca acc agg cgc act cga agc cgt aag atc gat tga           1150
Ala Lys Pro Ala Thr Arg Arg Thr Arg Ser Arg Lys Ile Asp  
285                 290                 295     
tagtcgcgta gtgtggtcgt ttcactagtc ttcgtattgt ttcttcgcaa taatattgga     1210
                                                                  

atattggact tttcctgtac agatcgagcg ctcggccgcg ctgtttcgca acgaagcgcg     1270
                                                                  

cgcgcgccca gcccgaagct tcagcgcgca tcgcgacatc gtcctcacat tcgtacgcgc     1330
                                                                  

gacgttcacg cctcgtcgta aacgcgctcg atcgatcgaa agactttaaa cacgccgaac     1390
                                                                  

gaaggcgcga acgagtccaa cg                                              1412
                         


<210> 12
<211> 298
<212> PRT
<213> Ostreococcus lucimarinus

<400> 12
Met Ala Gln Phe Pro Leu Val Ser Leu Cys Ala Phe Ala Val Tyr Gly 
1                 5                  10                  15     
Tyr Ala Thr Tyr Ala Tyr Ala Phe Glu Trp Ser His Ala Arg Thr Pro 
             20                  25                  30         
Gly Gly Leu Ala Asn Val Asp Ala Gln Arg Trp Ile Gly Asp Leu Ser 
         35                  40                  45             
Phe Ala Leu Pro Ala Cys Ala Thr Thr Ala Tyr Leu Met Phe Cys Leu 
     50                  55                  60                 
Val Gly Pro Arg Val Met Ala Lys Arg Glu Ala Phe Asp Pro Lys Gly 
65                   70                  75                  80 
Leu Met Leu Ala Tyr Asn Ala Tyr Gln Thr Ala Phe Asn Val Cys Val 
                 85                  90                  95     
Leu Gly Met Phe Ile Arg Glu Ile Val Thr Leu Lys Gln Pro Thr Trp 
            100                 105                 110         
Gly Ser Lys Met Pro Trp Ser Asp Lys Arg Ser Phe Asn Ile Leu Leu 
        115                 120                 125             
Gly Val Trp Phe His Tyr Asn Asn Lys Tyr Leu Glu Leu Leu Asp Thr 
    130                 135                 140                 
Ala Phe Met Ile Ala Arg Lys Lys Thr Asn Gln Leu Ser Phe Leu His 
145                 150                 155                 160 
Val Tyr His His Ala Leu Leu Ile Trp Ala Trp Trp Phe Val Cys His 
                165                 170                 175     
Leu Met Ala Thr Asn Asp Cys Val Asp Ala Tyr Phe Gly Ala Ala Cys 
            180                 185                 190         
Asn Ser Phe Ile His Ile Val Met Tyr Ser Tyr Tyr Leu Met Ala Ala 
        195                 200                 205             
Leu Gly Val Arg Cys Pro Trp Lys Arg Tyr Ile Thr Gln Ala Gln Met 
    210                 215                 220                 
Leu Gln Phe Val Ile Val Phe Val His Ala Val Phe Val Leu Arg Glu 
225                 230                 235                 240 
Lys His Cys Pro Val Ser Leu Pro Trp Ala Gln Met Phe Val Met Ala 
                245                 250                 255     
Asn Met Leu Val Leu Phe Gly Asn Phe Tyr Leu Lys Ala Tyr Ala Ala 
            260                 265                 270         
Lys Pro Ser Gly Lys Ser Ser Thr Arg Val Ser Ala Ala Lys Pro Ala 
        275                 280                 285             
Thr Arg Arg Thr Arg Ser Arg Lys Ile Asp 
    290                 295             

<210> 13
<211> 2077
<212> DNA
<213> Ostreococcus lucimarinus

<220>
<223> Delta-6 desaturase

<220>
<221> CDS
<222> (388)..(1836)

<400> 13
cgccgcgcgg cgacgttcaa cgtagcgaat tgaaacgaat tagcatgtgc gaataacgcg     60
                                                                  

agagacgatg attcgatcgc gacgaccacc gcgtcgaccg cgcgcgcggt cgcgcgaggc     120
                                                                  

gacattaaaa cgcgcgcgct cggcgctcgc gcgcgacgca gggagcacaa gatgatgaca     180
                                                                  

ctggcggctc gctttatcgt cacatcgcgt cgtcgcgcgc gcgcggcggc gtccgtgacc     240
                                                                  

gcgcgcaccg cgaaacaaac acgcgcgatc aaagtttcgc gcgcgccgca cgcctcgaag     300
                                                                  

acgcgcggcg acgacgtcag acgcgttcaa agcgacgaat tccacaaaac tgatcgttcg     360
                                                                  

ctttaaatcc tgattaaaac gcgagag atg tgc gtc gaa acg acc gaa ggc aca     414
                              Met Cys Val Glu Thr Thr Glu Gly Thr 
                                1               5
tcg cga acg atg gcg aac gaa cgc acg agc tcg tcg tcg tcg ctg agc       462
Ser Arg Thr Met Ala Asn Glu Arg Thr Ser Ser Ser Ser Ser Leu Ser 
 10                  15                  20                  25   
gaa ggc gga acg ccg acg gtg acg gtc ggg atg gga agc gaa gac gcg       510
Glu Gly Gly Thr Pro Thr Val Thr Val Gly Met Gly Ser Glu Asp Ala 
                 30                  35                  40  
ggg aag aag act cga aac gcg agc gtc acg gcg tgg acg aaa gag ttg       558
Gly Lys Lys Thr Arg Asn Ala Ser Val Thr Ala Trp Thr Lys Glu Leu 
             45                  50                  55  
gag ccg cac gcg atc gcg aag acg ttc gaa cgg cgg tac gtg acg atc       606
Glu Pro His Ala Ile Ala Lys Thr Phe Glu Arg Arg Tyr Val Thr Ile 
         60                  65                  70  
gaa ggc gtg gaa tac gat gtg acg gat ttt aag cat ccc gga gga tcg       654
Glu Gly Val Glu Tyr Asp Val Thr Asp Phe Lys His Pro Gly Gly Ser 
     75                  80                  85  
gtt att tat tac atg ctg tcg aac acg gga gcg gac gcg acg gag gct       702
Val Ile Tyr Tyr Met Leu Ser Asn Thr Gly Ala Asp Ala Thr Glu Ala 
 90                  95                 100                 105     
ttt aaa gag ttt cat tat cgg tcg aaa aag gcg cgc aag gcg ttg gcg       750
Phe Lys Glu Phe His Tyr Arg Ser Lys Lys Ala Arg Lys Ala Leu Ala 
                110                 115                 120     
gcg ttg ccg cat aag cca gtg gac gcg gcg acg cgg gaa ccg atc gaa       798
Ala Leu Pro His Lys Pro Val Asp Ala Ala Thr Arg Glu Pro Ile Glu 
            125                 130                 135     
gat gag gcg atg ctg aag gat ttc gcg cag tgg cgc aag gaa ttg gag       846
Asp Glu Ala Met Leu Lys Asp Phe Ala Gln Trp Arg Lys Glu Leu Glu 
        140                 145                 150     
cgt gag gga ttt ttt aag ccc tcg ccg gcg cac gtg gcg tat cga ttc       894
Arg Glu Gly Phe Phe Lys Pro Ser Pro Ala His Val Ala Tyr Arg Phe 
    155                 160                 165     
gcc gag ctc gcg gcg atg ttc gcg ctc ggc acg gcg ttg atg cac gcg       942
Ala Glu Leu Ala Ala Met Phe Ala Leu Gly Thr Ala Leu Met His Ala 
170                 175                 180                 185       
cgt tgg cac gtc gct tcc gtg atc gtg tac tcg tgt ttc ttc ggc gcg       990
Arg Trp His Val Ala Ser Val Ile Val Tyr Ser Cys Phe Phe Gly Ala 
                190                 195                 200     
cga tgc ggt tgg gtg cag cac gag ggt ggg cac aat tcg ttg act gga       1038
Arg Cys Gly Trp Val Gln His Glu Gly Gly His Asn Ser Leu Thr Gly 
            205                 210                 215     
aac att tgg tgg gac aag cga atc caa gcc ttc gcc gcg ggg ttc ggc       1086
Asn Ile Trp Trp Asp Lys Arg Ile Gln Ala Phe Ala Ala Gly Phe Gly 
        220                 225                 230     
ttg gcg tcg agt ggc gac atg tgg aac aac atg cac aac aag cat cac       1134
Leu Ala Ser Ser Gly Asp Met Trp Asn Asn Met His Asn Lys His His 
    235                 240                 245     
gcg acg ccc caa aag gtg cga cac gat atg gat ctc gac acc act ccc       1182
Ala Thr Pro Gln Lys Val Arg His Asp Met Asp Leu Asp Thr Thr Pro 
250                 255                 260                 265       
acg gtg gcg ttc ttc aac tcc gcg gtt gaa gaa aat cgc ccg cgg gga       1230
Thr Val Ala Phe Phe Asn Ser Ala Val Glu Glu Asn Arg Pro Arg Gly 
                270                 275                 280     
ttc agt aag ttg tgg ttg cgc ctt caa gcg tgg acc ttc gtg ccc gtg       1278
Phe Ser Lys Leu Trp Leu Arg Leu Gln Ala Trp Thr Phe Val Pro Val 
            285                 290                 295     
acg tcc ggt atg gtt ttg ttc ttc tgg atg ttc gtc ttg cac ccg cgt       1326
Thr Ser Gly Met Val Leu Phe Phe Trp Met Phe Val Leu His Pro Arg 
        300                 305                 310     
aac gcg ctg cga cgc aaa agc ttc gaa gaa gcg gct tgg atg ttt tcc       1374
Asn Ala Leu Arg Arg Lys Ser Phe Glu Glu Ala Ala Trp Met Phe Ser 
    315                 320                 325     
gcg cac gtc att cgc acg gcg gtt atc aaa gcc gtc acc ggc tac tcc       1422
Ala His Val Ile Arg Thr Ala Val Ile Lys Ala Val Thr Gly Tyr Ser 
330                 335                 340                 345       
tgg atc gcc tcg tac ggc ttg ttc gcg gcg acg atg tgg gcg agc gga       1470
Trp Ile Ala Ser Tyr Gly Leu Phe Ala Ala Thr Met Trp Ala Ser Gly 
                350                 355                 360     
tgt tac ttg ttc gcg cac ttt tcc acg tct cac acg cac ttg gat gtc       1518
Cys Tyr Leu Phe Ala His Phe Ser Thr Ser His Thr His Leu Asp Val 
            365                 370                 375     
gtg ccg agc gat aaa cac ctc tcg tgg gtg cga tac gcc gtc gat cac       1566
Val Pro Ser Asp Lys His Leu Ser Trp Val Arg Tyr Ala Val Asp His 
        380                 385                 390     
acg atc gac atc aat ccg aac aac agc gtc gtc aac tgg ttg atg ggc       1614
Thr Ile Asp Ile Asn Pro Asn Asn Ser Val Val Asn Trp Leu Met Gly 
    395                 400                 405     
tac ttg aac tgc caa gtc atc cat cac ctg ttc ccg gat atg cct cag       1662
Tyr Leu Asn Cys Gln Val Ile His His Leu Phe Pro Asp Met Pro Gln 
410                 415                 420                 425       
ttc cgc caa ccc gaa gtc tcc cgc cga ttc gtc ccg ttt gcg aag aag       1710
Phe Arg Gln Pro Glu Val Ser Arg Arg Phe Val Pro Phe Ala Lys Lys 
                430                 435                 440     
tgg aac tta aac tac aag gtc ttg acg tat tat ggg gcc tgg aag gcg       1758
Trp Asn Leu Asn Tyr Lys Val Leu Thr Tyr Tyr Gly Ala Trp Lys Ala 
            445                 450                 455     
acg ttc ggc aac ttg aac gac gtc ggg aag cac tat tac gtg cac gga       1806
Thr Phe Gly Asn Leu Asn Asp Val Gly Lys His Tyr Tyr Val His Gly 
        460                 465                 470     
tct cag cgc gtc aaa tca aag tcg gcg tga gtttcgatga gctcttcgcc         1856
Ser Gln Arg Val Lys Ser Lys Ser Ala                        
    475                 480   
ctcgcgatgt cgcctcgcgc gacgttctct catcttatcc catcttgcat catctcgcgc     1916
                                                                  

ggcacttcac tagctcgccg cgcgttatct ccaagattta ccgctttgtg aacgtttatt     1976
                                                                  

ctagcaacct ttagggtttt tttcgcgaac gagacgtcaa cttgtctgcc gcccatcgca     2036
                                                                  

acgttccgcc acaccacggc tcgccgacgc ccgctcgcgc g                         2077
                                              


<210> 14
<211> 482
<212> PRT
<213> Ostreococcus lucimarinus

<400> 14
Met Cys Val Glu Thr Thr Glu Gly Thr Ser Arg Thr Met Ala Asn Glu 
1                 5                  10                  15     
Arg Thr Ser Ser Ser Ser Ser Leu Ser Glu Gly Gly Thr Pro Thr Val 
             20                  25                  30         
Thr Val Gly Met Gly Ser Glu Asp Ala Gly Lys Lys Thr Arg Asn Ala 
         35                  40                  45             
Ser Val Thr Ala Trp Thr Lys Glu Leu Glu Pro His Ala Ile Ala Lys 
     50                  55                  60                 
Thr Phe Glu Arg Arg Tyr Val Thr Ile Glu Gly Val Glu Tyr Asp Val 
65                   70                  75                  80 
Thr Asp Phe Lys His Pro Gly Gly Ser Val Ile Tyr Tyr Met Leu Ser 
                 85                  90                  95     
Asn Thr Gly Ala Asp Ala Thr Glu Ala Phe Lys Glu Phe His Tyr Arg 
            100                 105                 110         
Ser Lys Lys Ala Arg Lys Ala Leu Ala Ala Leu Pro His Lys Pro Val 
        115                 120                 125             
Asp Ala Ala Thr Arg Glu Pro Ile Glu Asp Glu Ala Met Leu Lys Asp 
    130                 135                 140                 
Phe Ala Gln Trp Arg Lys Glu Leu Glu Arg Glu Gly Phe Phe Lys Pro 
145                 150                 155                 160 
Ser Pro Ala His Val Ala Tyr Arg Phe Ala Glu Leu Ala Ala Met Phe 
                165                 170                 175     
Ala Leu Gly Thr Ala Leu Met His Ala Arg Trp His Val Ala Ser Val 
            180                 185                 190         
Ile Val Tyr Ser Cys Phe Phe Gly Ala Arg Cys Gly Trp Val Gln His 
        195                 200                 205             
Glu Gly Gly His Asn Ser Leu Thr Gly Asn Ile Trp Trp Asp Lys Arg 
    210                 215                 220                 
Ile Gln Ala Phe Ala Ala Gly Phe Gly Leu Ala Ser Ser Gly Asp Met 
225                 230                 235                 240 
Trp Asn Asn Met His Asn Lys His His Ala Thr Pro Gln Lys Val Arg 
                245                 250                 255     
His Asp Met Asp Leu Asp Thr Thr Pro Thr Val Ala Phe Phe Asn Ser 
            260                 265                 270         
Ala Val Glu Glu Asn Arg Pro Arg Gly Phe Ser Lys Leu Trp Leu Arg 
        275                 280                 285             
Leu Gln Ala Trp Thr Phe Val Pro Val Thr Ser Gly Met Val Leu Phe 
    290                 295                 300                 
Phe Trp Met Phe Val Leu His Pro Arg Asn Ala Leu Arg Arg Lys Ser 
305                 310                 315                 320 
Phe Glu Glu Ala Ala Trp Met Phe Ser Ala His Val Ile Arg Thr Ala 
                325                 330                 335     
Val Ile Lys Ala Val Thr Gly Tyr Ser Trp Ile Ala Ser Tyr Gly Leu 
            340                 345                 350         
Phe Ala Ala Thr Met Trp Ala Ser Gly Cys Tyr Leu Phe Ala His Phe 
        355                 360                 365             
Ser Thr Ser His Thr His Leu Asp Val Val Pro Ser Asp Lys His Leu 
    370                 375                 380                 
Ser Trp Val Arg Tyr Ala Val Asp His Thr Ile Asp Ile Asn Pro Asn 
385                 390                 395                 400 
Asn Ser Val Val Asn Trp Leu Met Gly Tyr Leu Asn Cys Gln Val Ile 
                405                 410                 415     
His His Leu Phe Pro Asp Met Pro Gln Phe Arg Gln Pro Glu Val Ser 
            420                 425                 430         
Arg Arg Phe Val Pro Phe Ala Lys Lys Trp Asn Leu Asn Tyr Lys Val 
        435                 440                 445             
Leu Thr Tyr Tyr Gly Ala Trp Lys Ala Thr Phe Gly Asn Leu Asn Asp 
    450                 455                 460                 
Val Gly Lys His Tyr Tyr Val His Gly Ser Gln Arg Val Lys Ser Lys 
465                 470                 475                 480 
Ser Ala 
        

<210> 15
<211> 864
<212> DNA
<213> Ostreococcus lucimarinus

<220>
<223> Delta-6 elongase

<220>
<221> CDS
<222> (1)..(864)

<400> 15
atg ttg cga cac tac tgg aag gcg tgg gac tca atc att tcg aaa gta       48
Met Leu Arg His Tyr Trp Lys Ala Trp Asp Ser Ile Ile Ser Lys Val 
  1               5                  10                  15 
gtt ttc tcc tgc gct gac tgg tta ggc tgg gag ctc gac ccg ctg agc       96
Val Phe Ser Cys Ala Asp Trp Leu Gly Trp Glu Leu Asp Pro Leu Ser 
             20                  25                  30  
ccc acc acc tca cat tta ccc gcc ata acc tca cca act cct ctg atc       144
Pro Thr Thr Ser His Leu Pro Ala Ile Thr Ser Pro Thr Pro Leu Ile 
         35                  40                  45  
acc agc ctt ctc gtg tac ttg gtc acg gtc gtt gta tcg tac cgc gtc       192
Thr Ser Leu Leu Val Tyr Leu Val Thr Val Val Val Ser Tyr Arg Val 
     50                  55                  60  
tta tcc gca aca aca aac aca aag att tgg gat ccc aca tgg tta aaa       240
Leu Ser Ala Thr Thr Asn Thr Lys Ile Trp Asp Pro Thr Trp Leu Lys 
 65                  70                  75                  80   
gca tcg gtg att tgc cac aat gca ttc ctc ata tta cta agt ttg tat       288
Ala Ser Val Ile Cys His Asn Ala Phe Leu Ile Leu Leu Ser Leu Tyr 
                 85                  90                  95  
atg tgc atc ggt tgt atc gtc gaa gca tac aaa agt gga tat aag cta       336
Met Cys Ile Gly Cys Ile Val Glu Ala Tyr Lys Ser Gly Tyr Lys Leu 
            100                 105                 110     
tgg gga aat aag ttc aac gtg aat gaa aag cag ctt gcg ttt tac atc       384
Trp Gly Asn Lys Phe Asn Val Asn Glu Lys Gln Leu Ala Phe Tyr Ile 
        115                 120                 125     
tac ctc ttt tac gtg agc aag ata tat gag ttc gtt gat aca ttc ata       432
Tyr Leu Phe Tyr Val Ser Lys Ile Tyr Glu Phe Val Asp Thr Phe Ile 
    130                 135                 140     
atg cta ctg aag aga aac tta cgc caa gta agc ttt ttg cat gtc tac       480
Met Leu Leu Lys Arg Asn Leu Arg Gln Val Ser Phe Leu His Val Tyr 
145                 150                 155                 160       
cat cac agc act att tcg ttt ata tgg tgg atg atc gca cgt cga gct       528
His His Ser Thr Ile Ser Phe Ile Trp Trp Met Ile Ala Arg Arg Ala 
                165                 170                 175     
ccg ggt ggg gat gct tac ttc agt gca gca ctg aac tct tgg gta cac       576
Pro Gly Gly Asp Ala Tyr Phe Ser Ala Ala Leu Asn Ser Trp Val His 
            180                 185                 190     
gtg tgc atg tac acg tac tat ctg cta tcg gct ctg atc ggg aaa aac       624
Val Cys Met Tyr Thr Tyr Tyr Leu Leu Ser Ala Leu Ile Gly Lys Asn 
        195                 200                 205     
aac gac aaa cgt gtt aag tac ctt tgg tgg ggt cga cat ttg acg cag       672
Asn Asp Lys Arg Val Lys Tyr Leu Trp Trp Gly Arg His Leu Thr Gln 
    210                 215                 220     
atg cag atg ctt caa ttt cta tgc aat ctg tta cag gcc gtg tac tgt       720
Met Gln Met Leu Gln Phe Leu Cys Asn Leu Leu Gln Ala Val Tyr Cys 
225                 230                 235                 240       
gca tat ttt tca gaa tac ccg aag ttc ttg tca aag ata ctc ctt ttt       768
Ala Tyr Phe Ser Glu Tyr Pro Lys Phe Leu Ser Lys Ile Leu Leu Phe 
                245                 250                 255     
tac atg att agc ctg ctg gcg ctg ttt gga cat ttc tat tac tcc aag       816
Tyr Met Ile Ser Leu Leu Ala Leu Phe Gly His Phe Tyr Tyr Ser Lys 
            260                 265                 270     
cac atc gcc acc gcg aag ctc agg aaa aaa cac acc aag aag gcg tga       864
His Ile Ala Thr Ala Lys Leu Arg Lys Lys His Thr Lys Lys Ala  
        275                 280                 285     

<210> 16
<211> 287
<212> PRT
<213> Ostreococcus lucimarinus

<400> 16
Met Leu Arg His Tyr Trp Lys Ala Trp Asp Ser Ile Ile Ser Lys Val 
1                 5                  10                  15     
Val Phe Ser Cys Ala Asp Trp Leu Gly Trp Glu Leu Asp Pro Leu Ser 
             20                  25                  30         
Pro Thr Thr Ser His Leu Pro Ala Ile Thr Ser Pro Thr Pro Leu Ile 
         35                  40                  45             
Thr Ser Leu Leu Val Tyr Leu Val Thr Val Val Val Ser Tyr Arg Val 
     50                  55                  60                 
Leu Ser Ala Thr Thr Asn Thr Lys Ile Trp Asp Pro Thr Trp Leu Lys 
65                   70                  75                  80 
Ala Ser Val Ile Cys His Asn Ala Phe Leu Ile Leu Leu Ser Leu Tyr 
                 85                  90                  95     
Met Cys Ile Gly Cys Ile Val Glu Ala Tyr Lys Ser Gly Tyr Lys Leu 
            100                 105                 110         
Trp Gly Asn Lys Phe Asn Val Asn Glu Lys Gln Leu Ala Phe Tyr Ile 
        115                 120                 125             
Tyr Leu Phe Tyr Val Ser Lys Ile Tyr Glu Phe Val Asp Thr Phe Ile 
    130                 135                 140                 
Met Leu Leu Lys Arg Asn Leu Arg Gln Val Ser Phe Leu His Val Tyr 
145                 150                 155                 160 
His His Ser Thr Ile Ser Phe Ile Trp Trp Met Ile Ala Arg Arg Ala 
                165                 170                 175     
Pro Gly Gly Asp Ala Tyr Phe Ser Ala Ala Leu Asn Ser Trp Val His 
            180                 185                 190         
Val Cys Met Tyr Thr Tyr Tyr Leu Leu Ser Ala Leu Ile Gly Lys Asn 
        195                 200                 205             
Asn Asp Lys Arg Val Lys Tyr Leu Trp Trp Gly Arg His Leu Thr Gln 
    210                 215                 220                 
Met Gln Met Leu Gln Phe Leu Cys Asn Leu Leu Gln Ala Val Tyr Cys 
225                 230                 235                 240 
Ala Tyr Phe Ser Glu Tyr Pro Lys Phe Leu Ser Lys Ile Leu Leu Phe 
                245                 250                 255     
Tyr Met Ile Ser Leu Leu Ala Leu Phe Gly His Phe Tyr Tyr Ser Lys 
            260                 265                 270         
His Ile Ala Thr Ala Lys Leu Arg Lys Lys His Thr Lys Lys Ala 
        275                 280                 285         
        


<210>  17

<211>  903

<212>  DNA

<213>  Ostreococcus tauri



<220>

<221>  CDS

<222>  (1)..(903)

<223>  Delta-5-Elongase



<400>  17
atg agc gcc tcc ggt gcg ctg ctg ccc gcg atc gcg ttc gcc gcg tac       48
Met Ser Ala Ser Gly Ala Leu Leu Pro Ala Ile Ala Phe Ala Ala Tyr         
1               5                   10                  15              

gcg tac gcg acg tac gcc tac gcc ttt gag tgg tcg cac gcg aat ggc       96
Ala Tyr Ala Thr Tyr Ala Tyr Ala Phe Glu Trp Ser His Ala Asn Gly         
            20                  25                  30                  

atc gac aac gtc gac gcg cgc gag tgg atc ggt gcg ctg tcg ttg agg      144
Ile Asp Asn Val Asp Ala Arg Glu Trp Ile Gly Ala Leu Ser Leu Arg         
        35                  40                  45                      

ctc ccg gcg atc gcg acg acg atg tac ctg ttg ttc tgc ctg gtc gga      192
Leu Pro Ala Ile Ala Thr Thr Met Tyr Leu Leu Phe Cys Leu Val Gly         
    50                  55                  60                          

ccg agg ttg atg gcg aag cgc gag gcg ttc gac ccg aag ggg ttc atg      240
Pro Arg Leu Met Ala Lys Arg Glu Ala Phe Asp Pro Lys Gly Phe Met         
65                  70                  75                  80          

ctg gcg tac aat gcg tat cag acg gcg ttc aac gtc gtc gtg ctc ggg      288
Leu Ala Tyr Asn Ala Tyr Gln Thr Ala Phe Asn Val Val Val Leu Gly         
                85                  90                  95              

atg ttc gcg cga gag atc tcg ggg ctg ggg cag ccc gtg tgg ggg tca      336
Met Phe Ala Arg Glu Ile Ser Gly Leu Gly Gln Pro Val Trp Gly Ser         
            100                 105                 110                 

acc atg ccg tgg agc gat aga aaa tcg ttt aag atc ctc ctc ggg gtg      384
Thr Met Pro Trp Ser Asp Arg Lys Ser Phe Lys Ile Leu Leu Gly Val         
        115                 120                 125                     

tgg ttg cac tac aac aac caa tat ttg gag cta ttg gac act gtg ttc      432
Trp Leu His Tyr Asn Asn Gln Tyr Leu Glu Leu Leu Asp Thr Val Phe         
    130                 135                 140                         

atg gtt gcg cgc aag aag acg aag cag ttg agc ttc ttg cac gtt tat      480
Met Val Ala Arg Lys Lys Thr Lys Gln Leu Ser Phe Leu His Val Tyr         
145                 150                 155                 160         

cat cac gcc ctg ttg atc tgg gcg tgg tgg ttg gtg tgt cac ttg atg      528
His His Ala Leu Leu Ile Trp Ala Trp Trp Leu Val Cys His Leu Met         
                165                 170                 175             


gcc acg aac gat tgt atc gat gcc tac ttc ggc gcg gcg tgc aac tcg      576
Ala Thr Asn Asp Cys Ile Asp Ala Tyr Phe Gly Ala Ala Cys Asn Ser         
            180                 185                 190                 

ttc att cac atc gtg atg tac tcg tat tat ctc atg tcg gcg ctc ggc      624
Phe Ile His Ile Val Met Tyr Ser Tyr Tyr Leu Met Ser Ala Leu Gly         
        195                 200                 205                     

att cga tgc ccg tgg aag cga tac atc acc cag gct caa atg ctc caa      672
Ile Arg Cys Pro Trp Lys Arg Tyr Ile Thr Gln Ala Gln Met Leu Gln         
    210                 215                 220                         

ttc gtc att gtc ttc gcg cac gcc gtg ttc gtg ctg cgt cag aag cac      720
Phe Val Ile Val Phe Ala His Ala Val Phe Val Leu Arg Gln Lys His         
225                 230                 235                 240         

tgc ccg gtc acc ctt cct tgg gcg caa atg ttc gtc atg acg aac atg      768
Cys Pro Val Thr Leu Pro Trp Ala Gln Met Phe Val Met Thr Asn Met         
                245                 250                 255             

ctc gtg ctc ttc ggg aac ttc tac ctc aag gcg tac tcg aac aag tcg      816
Leu Val Leu Phe Gly Asn Phe Tyr Leu Lys Ala Tyr Ser Asn Lys Ser         
            260                 265                 270                 

cgc ggc gac ggc gcg agt tcc gtg aaa cca gcc gag acc acg cgc gcg      864
Arg Gly Asp Gly Ala Ser Ser Val Lys Pro Ala Glu Thr Thr Arg Ala         
        275                 280                 285                     

ccc agc gtg cga cgc acg cga tct cga aaa att gac taa                  903
Pro Ser Val Arg Arg Thr Arg Ser Arg Lys Ile Asp                         
    290                 295                 300                         


<210>  18

<211>  300

<212>  PRT

<213>  Ostreococcus tauri



<400>  18

Met Ser Ala Ser Gly Ala Leu Leu Pro Ala Ile Ala Phe Ala Ala Tyr 
1               5                   10                  15      

Ala Tyr Ala Thr Tyr Ala Tyr Ala Phe Glu Trp Ser His Ala Asn Gly 
            20                  25                  30          

Ile Asp Asn Val Asp Ala Arg Glu Trp Ile Gly Ala Leu Ser Leu Arg 

        35                  40                  45              

Leu Pro Ala Ile Ala Thr Thr Met Tyr Leu Leu Phe Cys Leu Val Gly 
    50                  55                  60                  

Pro Arg Leu Met Ala Lys Arg Glu Ala Phe Asp Pro Lys Gly Phe Met 
65                  70                  75                  80  

Leu Ala Tyr Asn Ala Tyr Gln Thr Ala Phe Asn Val Val Val Leu Gly 
                85                  90                  95      

Met Phe Ala Arg Glu Ile Ser Gly Leu Gly Gln Pro Val Trp Gly Ser 
            100                 105                 110         

Thr Met Pro Trp Ser Asp Arg Lys Ser Phe Lys Ile Leu Leu Gly Val 
        115                 120                 125             

Trp Leu His Tyr Asn Asn Gln Tyr Leu Glu Leu Leu Asp Thr Val Phe 
    130                 135                 140                 

Met Val Ala Arg Lys Lys Thr Lys Gln Leu Ser Phe Leu His Val Tyr 
145                 150                 155                 160 

His His Ala Leu Leu Ile Trp Ala Trp Trp Leu Val Cys His Leu Met 
                165                 170                 175     

Ala Thr Asn Asp Cys Ile Asp Ala Tyr Phe Gly Ala Ala Cys Asn Ser 
            180                 185                 190         

Phe Ile His Ile Val Met Tyr Ser Tyr Tyr Leu Met Ser Ala Leu Gly 
        195                 200                 205             

Ile Arg Cys Pro Trp Lys Arg Tyr Ile Thr Gln Ala Gln Met Leu Gln 
    210                 215                 220                 

Phe Val Ile Val Phe Ala His Ala Val Phe Val Leu Arg Gln Lys His 
225                 230                 235                 240 

Cys Pro Val Thr Leu Pro Trp Ala Gln Met Phe Val Met Thr Asn Met 
                245                 250                 255     

Leu Val Leu Phe Gly Asn Phe Tyr Leu Lys Ala Tyr Ser Asn Lys Ser 
            260                 265                 270         

Arg Gly Asp Gly Ala Ser Ser Val Lys Pro Ala Glu Thr Thr Arg Ala 
        275                 280                 285             

Pro Ser Val Arg Arg Thr Arg Ser Arg Lys Ile Asp 
    290                 295                 300 


<210>  19

<211>  879

<212>  DNA

<213>  Ostreococcus tauri



<220>

<221>  CDS

<222>  (1)..(879)

<223>  Delta-6-Elongase



<400>  19
atg agt ggc tta cgt gca ccc aac ttt tta cac aga ttc tgg aca aag       48
Met Ser Gly Leu Arg Ala Pro Asn Phe Leu His Arg Phe Trp Thr Lys         
1               5                   10                  15              

tgg gac tac gcg att tcc aaa gtc gtc ttc acg tgt gcc gac agt ttt       96
Trp Asp Tyr Ala Ile Ser Lys Val Val Phe Thr Cys Ala Asp Ser Phe         
            20                  25                  30                  

cag tgg gac atc ggg cca gtg agt tcg agt acg gcg cat tta ccc gcc      144
Gln Trp Asp Ile Gly Pro Val Ser Ser Ser Thr Ala His Leu Pro Ala         
        35                  40                  45                      

att gaa tcc cct acc cca ctg gtg act agc ctc ttg ttc tac tta gtc      192
Ile Glu Ser Pro Thr Pro Leu Val Thr Ser Leu Leu Phe Tyr Leu Val         
    50                  55                  60                          

aca gtt ttc ttg tgg tat ggt cgt tta acc agg agt tca gac aag aaa      240
Thr Val Phe Leu Trp Tyr Gly Arg Leu Thr Arg Ser Ser Asp Lys Lys         
65                  70                  75                  80          

att aga gag cct acg tgg tta aga aga ttc ata ata tgt cat aat gcg      288
Ile Arg Glu Pro Thr Trp Leu Arg Arg Phe Ile Ile Cys His Asn Ala         
                85                  90                  95              

ttc ttg ata gtc ctc agt ctt tac atg tgc ctt ggt tgt gtg gcc caa      336
Phe Leu Ile Val Leu Ser Leu Tyr Met Cys Leu Gly Cys Val Ala Gln         
            100                 105                 110                 

gcg tat cag aat gga tat act tta tgg ggt aat gaa ttc aag gcc acg      384
Ala Tyr Gln Asn Gly Tyr Thr Leu Trp Gly Asn Glu Phe Lys Ala Thr         
        115                 120                 125                     

gaa act cag ctt gct ctc tac att tac att ttt tac gta agt aaa ata      432
Glu Thr Gln Leu Ala Leu Tyr Ile Tyr Ile Phe Tyr Val Ser Lys Ile         
    130                 135                 140                         

tac gag ttt gta gat act tac att atg ctt ctc aag aat aac ttg cgg      480
Tyr Glu Phe Val Asp Thr Tyr Ile Met Leu Leu Lys Asn Asn Leu Arg         
145                 150                 155                 160         

caa gta agt ttc cta cac att tat cac cac agc acg att tcc ttt att      528
Gln Val Ser Phe Leu His Ile Tyr His His Ser Thr Ile Ser Phe Ile         
                165                 170                 175             

tgg tgg atc att gct cgg agg gct ccg ggt ggt gat gct tac ttc agc      576
Trp Trp Ile Ile Ala Arg Arg Ala Pro Gly Gly Asp Ala Tyr Phe Ser         
            180                 185                 190                 

gcg gcc ttg aac tca tgg gta cac gtg tgc atg tac acc tat tat cta      624
Ala Ala Leu Asn Ser Trp Val His Val Cys Met Tyr Thr Tyr Tyr Leu         
        195                 200                 205                     

tta tca acc ctt att gga aaa gaa gat cct aag cgt tcc aac tac ctt      672
Leu Ser Thr Leu Ile Gly Lys Glu Asp Pro Lys Arg Ser Asn Tyr Leu         
    210                 215                 220                         

tgg tgg ggt cgc cac cta acg caa atg cag atg ctt cag ttt ttc ttc      720
Trp Trp Gly Arg His Leu Thr Gln Met Gln Met Leu Gln Phe Phe Phe         
225                 230                 235                 240         

aac gta ctt caa gcg ttg tac tgc gct tcg ttc tct acg tat ccc aag      768
Asn Val Leu Gln Ala Leu Tyr Cys Ala Ser Phe Ser Thr Tyr Pro Lys         
                245                 250                 255             

ttt ttg tcc aaa att ctg ctc gtc tat atg atg agc ctt ctc ggc ttg      816
Phe Leu Ser Lys Ile Leu Leu Val Tyr Met Met Ser Leu Leu Gly Leu         
            260                 265                 270                 

ttt ggg cat ttc tac tat tcc aag cac ata gca gca gct aag ctc cag      864
Phe Gly His Phe Tyr Tyr Ser Lys His Ile Ala Ala Ala Lys Leu Gln         
        275                 280                 285                     

aaa aaa cag cag tga                                                  879
Lys Lys Gln Gln                                                         
    290                                                                 


<210>  20

<211>  292

<212>  PRT

<213>  Ostreococcus tauri



<400>  20

Met Ser Gly Leu Arg Ala Pro Asn Phe Leu His Arg Phe Trp Thr Lys 
1               5                   10                  15      

Trp Asp Tyr Ala Ile Ser Lys Val Val Phe Thr Cys Ala Asp Ser Phe 
            20                  25                  30          

Gln Trp Asp Ile Gly Pro Val Ser Ser Ser Thr Ala His Leu Pro Ala 
        35                  40                  45              

Ile Glu Ser Pro Thr Pro Leu Val Thr Ser Leu Leu Phe Tyr Leu Val 
    50                  55                  60                  

Thr Val Phe Leu Trp Tyr Gly Arg Leu Thr Arg Ser Ser Asp Lys Lys 
65                  70                  75                  80  

Ile Arg Glu Pro Thr Trp Leu Arg Arg Phe Ile Ile Cys His Asn Ala 
                85                  90                  95      

Phe Leu Ile Val Leu Ser Leu Tyr Met Cys Leu Gly Cys Val Ala Gln 
            100                 105                 110         

Ala Tyr Gln Asn Gly Tyr Thr Leu Trp Gly Asn Glu Phe Lys Ala Thr 
        115                 120                 125             


Glu Thr Gln Leu Ala Leu Tyr Ile Tyr Ile Phe Tyr Val Ser Lys Ile 
    130                 135                 140                 

Tyr Glu Phe Val Asp Thr Tyr Ile Met Leu Leu Lys Asn Asn Leu Arg 
145                 150                 155                 160 

Gln Val Ser Phe Leu His Ile Tyr His His Ser Thr Ile Ser Phe Ile 
                165                 170                 175     

Trp Trp Ile Ile Ala Arg Arg Ala Pro Gly Gly Asp Ala Tyr Phe Ser 
            180                 185                 190         

Ala Ala Leu Asn Ser Trp Val His Val Cys Met Tyr Thr Tyr Tyr Leu 
        195                 200                 205             

Leu Ser Thr Leu Ile Gly Lys Glu Asp Pro Lys Arg Ser Asn Tyr Leu 
    210                 215                 220                 

Trp Trp Gly Arg His Leu Thr Gln Met Gln Met Leu Gln Phe Phe Phe 
225                 230                 235                 240 

Asn Val Leu Gln Ala Leu Tyr Cys Ala Ser Phe Ser Thr Tyr Pro Lys 
                245                 250                 255     

Phe Leu Ser Lys Ile Leu Leu Val Tyr Met Met Ser Leu Leu Gly Leu 
            260                 265                 270         

Phe Gly His Phe Tyr Tyr Ser Lys His Ile Ala Ala Ala Lys Leu Gln 
        275                 280                 285             

Lys Lys Gln Gln 
    290         


<210>  21

<211>  879

<212>  DNA

<213>  Ostreococcus tauri



<220>

<221>  CDS

<222>  (1)..(879)

<223>  Delta-6-Elongase



<400>  21
atg agt ggc tta cgt gca ccc aac ttt tta cac aga ttc tgg aca aag       48
Met Ser Gly Leu Arg Ala Pro Asn Phe Leu His Arg Phe Trp Thr Lys         
1               5                   10                  15              

tgg gac tac gcg att tcc aaa gtc gtc ttc acg tgt gcc gac agt ttt       96
Trp Asp Tyr Ala Ile Ser Lys Val Val Phe Thr Cys Ala Asp Ser Phe         
            20                  25                  30                  

cag tgg gac atc ggg cca gtg agt tcg agt acg gcg cat tta ccc gcc      144
Gln Trp Asp Ile Gly Pro Val Ser Ser Ser Thr Ala His Leu Pro Ala         
        35                  40                  45                      

att gaa tcc cct acc cca ctg gtg act agc ctc ttg ttc tac tta gtc      192
Ile Glu Ser Pro Thr Pro Leu Val Thr Ser Leu Leu Phe Tyr Leu Val         
    50                  55                  60                          

aca gtt ttc ttg tgg tat ggt cgt tta acc agg agt tca gac aag aaa      240
Thr Val Phe Leu Trp Tyr Gly Arg Leu Thr Arg Ser Ser Asp Lys Lys         
65                  70                  75                  80          

att aga gag cct acg tgg tta aga aga ttc ata ata tgt cat aat gcg      288
Ile Arg Glu Pro Thr Trp Leu Arg Arg Phe Ile Ile Cys His Asn Ala         
                85                  90                  95              

ttc ttg ata gtc ctc agt ctt tac atg tgc ctt ggt tgt gtg gcc caa      336
Phe Leu Ile Val Leu Ser Leu Tyr Met Cys Leu Gly Cys Val Ala Gln         
            100                 105                 110                 

gcg tat cag aat gga tat act tta tgg ggt aat gaa ttc aag gcc acg      384
Ala Tyr Gln Asn Gly Tyr Thr Leu Trp Gly Asn Glu Phe Lys Ala Thr         
        115                 120                 125                     

gaa act cag ctt gct ctc tac att tac att ttt tac gta agt aaa ata      432
Glu Thr Gln Leu Ala Leu Tyr Ile Tyr Ile Phe Tyr Val Ser Lys Ile         
    130                 135                 140                         

tac gag ttt gta gat act tac att atg ctt ctc aag aat aac ttg cgg      480
Tyr Glu Phe Val Asp Thr Tyr Ile Met Leu Leu Lys Asn Asn Leu Arg         
145                 150                 155                 160         

caa gta aga ttc cta cac act tat cac cac agc acg att tcc ttt att      528
Gln Val Arg Phe Leu His Thr Tyr His His Ser Thr Ile Ser Phe Ile         
                165                 170                 175             

tgg tgg atc att gct cgg agg gct ccg ggt ggt gat gct tac ttc agc      576
Trp Trp Ile Ile Ala Arg Arg Ala Pro Gly Gly Asp Ala Tyr Phe Ser         
            180                 185                 190                 

gcg gcc ttg aac tca tgg gta cac gtg tgc atg tac acc tat tat cta      624
Ala Ala Leu Asn Ser Trp Val His Val Cys Met Tyr Thr Tyr Tyr Leu         
        195                 200                 205                     

tta tca acc ctt att gga aaa gaa gat cct aag cgt tcc aac tac ctt      672
Leu Ser Thr Leu Ile Gly Lys Glu Asp Pro Lys Arg Ser Asn Tyr Leu         
    210                 215                 220                         

tgg tgg ggt cgc cac cta acg caa atg cag atg ctt cag ttt ttc ttc      720
Trp Trp Gly Arg His Leu Thr Gln Met Gln Met Leu Gln Phe Phe Phe         
225                 230                 235                 240         

aac gta ctt caa gcg ttg tac tgc gct tcg ttc tct acg tat ccc aag      768
Asn Val Leu Gln Ala Leu Tyr Cys Ala Ser Phe Ser Thr Tyr Pro Lys         
                245                 250                 255             

ttt ttg tcc aaa att ctg ctc gtc tat atg atg agc ctt ctc ggc ttg      816
Phe Leu Ser Lys Ile Leu Leu Val Tyr Met Met Ser Leu Leu Gly Leu         
            260                 265                 270                 

ttt ggg cat ttc tac tat tcc aag cac ata gca gca gct aag ctc cag      864
Phe Gly His Phe Tyr Tyr Ser Lys His Ile Ala Ala Ala Lys Leu Gln         
        275                 280                 285                     

aaa aaa cag cag tga                                                  879
Lys Lys Gln Gln                                                         
    290                                                                 


<210>  22

<211>  292

<212>  PRT

<213>  Ostreococcus tauri



<400>  22

Met Ser Gly Leu Arg Ala Pro Asn Phe Leu His Arg Phe Trp Thr Lys 
1               5                   10                  15      

Trp Asp Tyr Ala Ile Ser Lys Val Val Phe Thr Cys Ala Asp Ser Phe 
            20                  25                  30          

Gln Trp Asp Ile Gly Pro Val Ser Ser Ser Thr Ala His Leu Pro Ala 
        35                  40                  45              


Ile Glu Ser Pro Thr Pro Leu Val Thr Ser Leu Leu Phe Tyr Leu Val 
    50                  55                  60                  

Thr Val Phe Leu Trp Tyr Gly Arg Leu Thr Arg Ser Ser Asp Lys Lys 
65                  70                  75                  80  


Ile Arg Glu Pro Thr Trp Leu Arg Arg Phe Ile Ile Cys His Asn Ala 
                85                  90                  95      

Phe Leu Ile Val Leu Ser Leu Tyr Met Cys Leu Gly Cys Val Ala Gln 
            100                 105                 110         

Ala Tyr Gln Asn Gly Tyr Thr Leu Trp Gly Asn Glu Phe Lys Ala Thr 
        115                 120                 125             

Glu Thr Gln Leu Ala Leu Tyr Ile Tyr Ile Phe Tyr Val Ser Lys Ile 
    130                 135                 140                 

Tyr Glu Phe Val Asp Thr Tyr Ile Met Leu Leu Lys Asn Asn Leu Arg 
145                 150                 155                 160 

Gln Val Arg Phe Leu His Thr Tyr His His Ser Thr Ile Ser Phe Ile 
                165                 170                 175     

Trp Trp Ile Ile Ala Arg Arg Ala Pro Gly Gly Asp Ala Tyr Phe Ser 
            180                 185                 190         

Ala Ala Leu Asn Ser Trp Val His Val Cys Met Tyr Thr Tyr Tyr Leu 
        195                 200                 205             

Leu Ser Thr Leu Ile Gly Lys Glu Asp Pro Lys Arg Ser Asn Tyr Leu 
    210                 215                 220                 

Trp Trp Gly Arg His Leu Thr Gln Met Gln Met Leu Gln Phe Phe Phe 
225                 230                 235                 240 

Asn Val Leu Gln Ala Leu Tyr Cys Ala Ser Phe Ser Thr Tyr Pro Lys 
                245                 250                 255     

Phe Leu Ser Lys Ile Leu Leu Val Tyr Met Met Ser Leu Leu Gly Leu 
            260                 265                 270         

Phe Gly His Phe Tyr Tyr Ser Lys His Ile Ala Ala Ala Lys Leu Gln 
        275                 280                 285             

Lys Lys Gln Gln 
    290         


<210>  23

<211>  1611

<212>  DNA

<213>  Ostreococcus tauri



<220>

<221>  CDS

<222>  (1)..(1611)

<223>  Delta-4-Desaturase


<400>  23
atg tac ctc gga cgc ggc cgt ctc gag agc ggg acg acg cga ggg atg       48
Met Tyr Leu Gly Arg Gly Arg Leu Glu Ser Gly Thr Thr Arg Gly Met         
1               5                   10                  15              

atg cgg acg cac gcg cgg cga ccg tcg acg acg tcg aat ccg tgc gcg       96
Met Arg Thr His Ala Arg Arg Pro Ser Thr Thr Ser Asn Pro Cys Ala         
            20                  25                  30                  

cgg tca cgc gtg cgt aag acg acg gag cga tcg ctc gcg cga gtg cga      144
Arg Ser Arg Val Arg Lys Thr Thr Glu Arg Ser Leu Ala Arg Val Arg         
        35                  40                  45                      

cga tcg acg agt gag aag gga agc gcg ctc gtg ctc gag cga gag agc      192
Arg Ser Thr Ser Glu Lys Gly Ser Ala Leu Val Leu Glu Arg Glu Ser         
    50                  55                  60                          

gaa cgg gag aag gag gag gga ggg aaa gcg cga gcg gag gga ttg cga      240
Glu Arg Glu Lys Glu Glu Gly Gly Lys Ala Arg Ala Glu Gly Leu Arg         
65                  70                  75                  80          

ttc caa cgc ccg gac gtc gcc gcg ccg ggg gga gcg gat cct tgg aac      288
Phe Gln Arg Pro Asp Val Ala Ala Pro Gly Gly Ala Asp Pro Trp Asn         
                85                  90                  95              

gac gag aag tgg aca aag acc aag tgg acg gta ttc aga gac gtc gcg      336
Asp Glu Lys Trp Thr Lys Thr Lys Trp Thr Val Phe Arg Asp Val Ala         
            100                 105                 110                 

tac gat ctc gat cct ttc ttc gct cga cac ccc gga gga gac tgg ctc      384
Tyr Asp Leu Asp Pro Phe Phe Ala Arg His Pro Gly Gly Asp Trp Leu         
        115                 120                 125                     

ctg aac ttg gcc gtg gga cga gac tgc acc gcg ctc atc gaa tcc tat      432
Leu Asn Leu Ala Val Gly Arg Asp Cys Thr Ala Leu Ile Glu Ser Tyr         
    130                 135                 140                         

cac ttg cga cca gag gtg gcg acg gct cgt ttc aga atg ctg ccc aaa      480
His Leu Arg Pro Glu Val Ala Thr Ala Arg Phe Arg Met Leu Pro Lys         
145                 150                 155                 160         

ctc gag gat ttt ccc gtc gag gcc gtg ccc aag tcc ccg aga ccg aac      528
Leu Glu Asp Phe Pro Val Glu Ala Val Pro Lys Ser Pro Arg Pro Asn         
                165                 170                 175             

gat tcg ccg tta tac aac aac att cgc aac cga gtc cgc gaa gag ctc      576
Asp Ser Pro Leu Tyr Asn Asn Ile Arg Asn Arg Val Arg Glu Glu Leu         
            180                 185                 190                 

ttc cca gag gag gga aag aat atg cac aga cag ggc ggc gac cac ggc      624
Phe Pro Glu Glu Gly Lys Asn Met His Arg Gln Gly Gly Asp His Gly         
        195                 200                 205                     

gac ggt gac gat tct ggg ttt cgc cgc ctt ttg ctt atg ccg tgt acc      672
Asp Gly Asp Asp Ser Gly Phe Arg Arg Leu Leu Leu Met Pro Cys Thr         
    210                 215                 220                         

tat tcc ctt ccg ggg gtt cct ttc cgg ctg cct cct cgg gtc tcg cgg      720
Tyr Ser Leu Pro Gly Val Pro Phe Arg Leu Pro Pro Arg Val Ser Arg         
225                 230                 235                 240         

ggg cgt gga ttg gtc tca cga ttc agg cac tgc gcc aac cac ggc gcg      768
Gly Arg Gly Leu Val Ser Arg Phe Arg His Cys Ala Asn His Gly Ala         
                245                 250                 255             

atg tct cct tcg ccg gcc gtt aac ggc gtc ctc ggt ttg acg aac gat      816
Met Ser Pro Ser Pro Ala Val Asn Gly Val Leu Gly Leu Thr Asn Asp         
            260                 265                 270                 

ctc atc ggc ggc tcg tcc ttg atg tgg aga tat cac cac caa gtc agc      864
Leu Ile Gly Gly Ser Ser Leu Met Trp Arg Tyr His His Gln Val Ser         
        275                 280                 285                     

cac cac att cat tgc aac gac aac gcc atg gat caa gac gtg tac acg      912
His His Ile His Cys Asn Asp Asn Ala Met Asp Gln Asp Val Tyr Thr         
    290                 295                 300                         

gcg atg cca tta ttg cgt ttc gac gct cgc cgg ccc aag tcc tgg tac      960
Ala Met Pro Leu Leu Arg Phe Asp Ala Arg Arg Pro Lys Ser Trp Tyr         
305                 310                 315                 320         

cat cgc ttc cag cag tgg tac atg ttt tta gcg ttc ccg ttg ttg cag     1008
His Arg Phe Gln Gln Trp Tyr Met Phe Leu Ala Phe Pro Leu Leu Gln         
                325                 330                 335             

gtt gcc ttc caa gtc gga gac att gcc gca ctg ttc acg cgt gat acc     1056
Val Ala Phe Gln Val Gly Asp Ile Ala Ala Leu Phe Thr Arg Asp Thr         
            340                 345                 350                 

gaa ggc gct aag ctt cac ggg gcg acg acg tgg gag ctt acc acg gtt     1104
Glu Gly Ala Lys Leu His Gly Ala Thr Thr Trp Glu Leu Thr Thr Val         
        355                 360                 365                     

gtc ctc ggt aag att gtg cac ttc ggt ctt ttg ttg ggg ccg ttg atg     1152
Val Leu Gly Lys Ile Val His Phe Gly Leu Leu Leu Gly Pro Leu Met         
    370                 375                 380                         

aac cac gcg gtg agt tct gtt ttg ctg ggg atc gtc ggt ttc atg gcg     1200
Asn His Ala Val Ser Ser Val Leu Leu Gly Ile Val Gly Phe Met Ala         
385                 390                 395                 400         

tgc caa ggt ata gtt ctg gcg tgc acg ttt gct gtg agt cac aat gtc     1248
Cys Gln Gly Ile Val Leu Ala Cys Thr Phe Ala Val Ser His Asn Val         
                405                 410                 415             

gcg gag gcg aag ata cct gag gac acc gga gga gaa gcc tgg gag aga     1296
Ala Glu Ala Lys Ile Pro Glu Asp Thr Gly Gly Glu Ala Trp Glu Arg         
            420                 425                 430                 

gat tgg ggt gtc cag cag ttg gtg act agc gcc gac tgg ggt gga aag     1344
Asp Trp Gly Val Gln Gln Leu Val Thr Ser Ala Asp Trp Gly Gly Lys         
        435                 440                 445                     

ata ggt aac ttc ttc acg ggt ggc ctc aac ttg caa gtt gag cac cac     1392
Ile Gly Asn Phe Phe Thr Gly Gly Leu Asn Leu Gln Val Glu His His         
    450                 455                 460                         

ttg ttt ccg gcg att tgc ttc gtc cac tac ccg gac atc gcg aag atc     1440
Leu Phe Pro Ala Ile Cys Phe Val His Tyr Pro Asp Ile Ala Lys Ile         
465                 470                 475                 480         

gtg aag gaa gaa gcg gcc aag ctc aac atc cct tac gcg tct tac agg     1488
Val Lys Glu Glu Ala Ala Lys Leu Asn Ile Pro Tyr Ala Ser Tyr Arg         
                485                 490                 495             

act ctt cct ggt att ttc gtc caa ttc tgg aga ttt atg aag gac atg     1536
Thr Leu Pro Gly Ile Phe Val Gln Phe Trp Arg Phe Met Lys Asp Met         
            500                 505                 510                 

ggc acg gct gag caa att ggt gaa gtt cca ttg ccg aag att ccc aac     1584
Gly Thr Ala Glu Gln Ile Gly Glu Val Pro Leu Pro Lys Ile Pro Asn         
        515                 520                 525                     

ccg cag ctc gcg ccg aag ctc gct tag                                 1611
Pro Gln Leu Ala Pro Lys Leu Ala                                         
    530                 535                                             


<210>  24

<211>  536

<212>  PRT

<213>  Ostreococcus tauri



<400>  24

Met Tyr Leu Gly Arg Gly Arg Leu Glu Ser Gly Thr Thr Arg Gly Met 
1               5                   10                  15      

Met Arg Thr His Ala Arg Arg Pro Ser Thr Thr Ser Asn Pro Cys Ala 
            20                  25                  30          

Arg Ser Arg Val Arg Lys Thr Thr Glu Arg Ser Leu Ala Arg Val Arg 
        35                  40                  45              

Arg Ser Thr Ser Glu Lys Gly Ser Ala Leu Val Leu Glu Arg Glu Ser 
    50                  55                  60                  

Glu Arg Glu Lys Glu Glu Gly Gly Lys Ala Arg Ala Glu Gly Leu Arg 
65                  70                  75                  80  

Phe Gln Arg Pro Asp Val Ala Ala Pro Gly Gly Ala Asp Pro Trp Asn 
                85                  90                  95      

Asp Glu Lys Trp Thr Lys Thr Lys Trp Thr Val Phe Arg Asp Val Ala 
            100                 105                 110         

Tyr Asp Leu Asp Pro Phe Phe Ala Arg His Pro Gly Gly Asp Trp Leu 
        115                 120                 125             

Leu Asn Leu Ala Val Gly Arg Asp Cys Thr Ala Leu Ile Glu Ser Tyr 
    130                 135                 140                 

His Leu Arg Pro Glu Val Ala Thr Ala Arg Phe Arg Met Leu Pro Lys 
145                 150                 155                 160 

Leu Glu Asp Phe Pro Val Glu Ala Val Pro Lys Ser Pro Arg Pro Asn 
                165                 170                 175     

Asp Ser Pro Leu Tyr Asn Asn Ile Arg Asn Arg Val Arg Glu Glu Leu 
            180                 185                 190         

Phe Pro Glu Glu Gly Lys Asn Met His Arg Gln Gly Gly Asp His Gly 
        195                 200                 205             

Asp Gly Asp Asp Ser Gly Phe Arg Arg Leu Leu Leu Met Pro Cys Thr 
    210                 215                 220                 

Tyr Ser Leu Pro Gly Val Pro Phe Arg Leu Pro Pro Arg Val Ser Arg 
225                 230                 235                 240 

Gly Arg Gly Leu Val Ser Arg Phe Arg His Cys Ala Asn His Gly Ala 
                245                 250                 255     

Met Ser Pro Ser Pro Ala Val Asn Gly Val Leu Gly Leu Thr Asn Asp 
            260                 265                 270         

Leu Ile Gly Gly Ser Ser Leu Met Trp Arg Tyr His His Gln Val Ser 
        275                 280                 285             

His His Ile His Cys Asn Asp Asn Ala Met Asp Gln Asp Val Tyr Thr 
    290                 295                 300                 

Ala Met Pro Leu Leu Arg Phe Asp Ala Arg Arg Pro Lys Ser Trp Tyr 
305                 310                 315                 320 

His Arg Phe Gln Gln Trp Tyr Met Phe Leu Ala Phe Pro Leu Leu Gln 
                325                 330                 335     

Val Ala Phe Gln Val Gly Asp Ile Ala Ala Leu Phe Thr Arg Asp Thr 
            340                 345                 350         

Glu Gly Ala Lys Leu His Gly Ala Thr Thr Trp Glu Leu Thr Thr Val 
        355                 360                 365             

Val Leu Gly Lys Ile Val His Phe Gly Leu Leu Leu Gly Pro Leu Met 
    370                 375                 380                 

Asn His Ala Val Ser Ser Val Leu Leu Gly Ile Val Gly Phe Met Ala 
385                 390                 395                 400 

Cys Gln Gly Ile Val Leu Ala Cys Thr Phe Ala Val Ser His Asn Val 
                405                 410                 415     

Ala Glu Ala Lys Ile Pro Glu Asp Thr Gly Gly Glu Ala Trp Glu Arg 
            420                 425                 430         

Asp Trp Gly Val Gln Gln Leu Val Thr Ser Ala Asp Trp Gly Gly Lys 
        435                 440                 445             

Ile Gly Asn Phe Phe Thr Gly Gly Leu Asn Leu Gln Val Glu His His 
    450                 455                 460                 

Leu Phe Pro Ala Ile Cys Phe Val His Tyr Pro Asp Ile Ala Lys Ile 
465                 470                 475                 480 

Val Lys Glu Glu Ala Ala Lys Leu Asn Ile Pro Tyr Ala Ser Tyr Arg 
                485                 490                 495     


Thr Leu Pro Gly Ile Phe Val Gln Phe Trp Arg Phe Met Lys Asp Met 
            500                 505                 510         

Gly Thr Ala Glu Gln Ile Gly Glu Val Pro Leu Pro Lys Ile Pro Asn 
        515                 520                 525             

Pro Gln Leu Ala Pro Lys Leu Ala 
    530                 535     


<210>  25

<211>  606

<212>  DNA

<213>  Ostreococcus tauri



<220>

<221>  CDS

<222>  (1)..(606)

<223>  Delta-5-Desaturase



<400>  25
atg tac ggt ttg cta tcg ctc aag tcg tgc ttc gtc gac gat ttc aac       48
Met Tyr Gly Leu Leu Ser Leu Lys Ser Cys Phe Val Asp Asp Phe Asn         
1               5                   10                  15              

gcc tac ttc tcc gga cgc atc ggc tgg gtc aag gtg atg aag ttc acc       96
Ala Tyr Phe Ser Gly Arg Ile Gly Trp Val Lys Val Met Lys Phe Thr         
            20                  25                  30                  

cgc ggc gag gcg atc gca ttt tgg ggc acc aag ctc ttg tgg gcc gcg      144
Arg Gly Glu Ala Ile Ala Phe Trp Gly Thr Lys Leu Leu Trp Ala Ala         
        35                  40                  45                      

tat tac ctc gcg ttg ccg cta aag atg tcg cat cgg ccg ctc gga gaa      192
Tyr Tyr Leu Ala Leu Pro Leu Lys Met Ser His Arg Pro Leu Gly Glu         
    50                  55                  60                          

ctc ctc gca ctc tgg gcc gtc acc gag ttc gtc acc gga tgg ctg ttg      240

Leu Leu Ala Leu Trp Ala Val Thr Glu Phe Val Thr Gly Trp Leu Leu         
65                  70                  75                  80          

gcg ttc atg ttc caa gtc gcc cac gtc gtc ggc gag gtt cac ttc ttc      288
Ala Phe Met Phe Gln Val Ala His Val Val Gly Glu Val His Phe Phe         
                85                  90                  95              

acc ctc gac gcg aag aac cgc gtg aac ttg gga tgg gga gag gca cag      336
Thr Leu Asp Ala Lys Asn Arg Val Asn Leu Gly Trp Gly Glu Ala Gln         
            100                 105                 110                 

ctc atg tcg agc gcg gat ttc gcc cac gga tcc aag ttt tgg acg cac      384
Leu Met Ser Ser Ala Asp Phe Ala His Gly Ser Lys Phe Trp Thr His         
        115                 120                 125                     

ttc tcc gga ggc tta aac tac caa gtc gtc cac cat ctc ttc ccg ggc      432
Phe Ser Gly Gly Leu Asn Tyr Gln Val Val His His Leu Phe Pro Gly         
    130                 135                 140                         

gtc tgc cac gtg cac tat ccc gcg ctc gcg cca att att aag gcg gca      480
Val Cys His Val His Tyr Pro Ala Leu Ala Pro Ile Ile Lys Ala Ala         
145                 150                 155                 160         

gct gag aag cac ggc ctc cac tac cag att tac ccc acg ttt tgg tcc      528
Ala Glu Lys His Gly Leu His Tyr Gln Ile Tyr Pro Thr Phe Trp Ser         
                165                 170                 175             

gcc ctg cgc gcg cac ttc cgg cac ctc gcc aac gtc ggc cgc gcc gcg      576
Ala Leu Arg Ala His Phe Arg His Leu Ala Asn Val Gly Arg Ala Ala         
            180                 185                 190                 

tac gta ccg tcc ctc caa acc gtc gga tga                              606

Tyr Val Pro Ser Leu Gln Thr Val Gly                                     
        195                 200                                         



<210>  26

<211>  201

<212>  PRT

<213>  Ostreococcus tauri



<400>  26

Met Tyr Gly Leu Leu Ser Leu Lys Ser Cys Phe Val Asp Asp Phe Asn 
1               5                   10                  15      

Ala Tyr Phe Ser Gly Arg Ile Gly Trp Val Lys Val Met Lys Phe Thr 
            20                  25                  30          

Arg Gly Glu Ala Ile Ala Phe Trp Gly Thr Lys Leu Leu Trp Ala Ala 
        35                  40                  45              

Tyr Tyr Leu Ala Leu Pro Leu Lys Met Ser His Arg Pro Leu Gly Glu 
    50                  55                  60                  

Leu Leu Ala Leu Trp Ala Val Thr Glu Phe Val Thr Gly Trp Leu Leu 
65                  70                  75                  80  

Ala Phe Met Phe Gln Val Ala His Val Val Gly Glu Val His Phe Phe 
                85                  90                  95      

Thr Leu Asp Ala Lys Asn Arg Val Asn Leu Gly Trp Gly Glu Ala Gln 
            100                 105                 110         

Leu Met Ser Ser Ala Asp Phe Ala His Gly Ser Lys Phe Trp Thr His 
        115                 120                 125             

Phe Ser Gly Gly Leu Asn Tyr Gln Val Val His His Leu Phe Pro Gly 
    130                 135                 140                 

Val Cys His Val His Tyr Pro Ala Leu Ala Pro Ile Ile Lys Ala Ala 
145                 150                 155                 160 

Ala Glu Lys His Gly Leu His Tyr Gln Ile Tyr Pro Thr Phe Trp Ser 
                165                 170                 175     

Ala Leu Arg Ala His Phe Arg His Leu Ala Asn Val Gly Arg Ala Ala 
            180                 185                 190         

Tyr Val Pro Ser Leu Gln Thr Val Gly 
        195                 200     


<210>  27

<211>  714

<212>  DNA

<213>  Ostreococcus tauri



<220>

<221>  CDS

<222>  (1)..(714)

<223>  Delta-5-Desaturase



<400>  27
atg gtg agc cat cac tcg tac tgt aac gac gcg gat ttg gat cag gat       48
Met Val Ser His His Ser Tyr Cys Asn Asp Ala Asp Leu Asp Gln Asp         
1               5                   10                  15              

gtg tac acc gca ctg ccg ctc ctg cgc ctg gac ccg tct cag gag ttg       96
Val Tyr Thr Ala Leu Pro Leu Leu Arg Leu Asp Pro Ser Gln Glu Leu         
            20                  25                  30                  

aag tgg ttt cat cga tac cag gcg ttt tac gcc ccg ctc atg tgg ccg      144
Lys Trp Phe His Arg Tyr Gln Ala Phe Tyr Ala Pro Leu Met Trp Pro         
        35                  40                  45                      


ttt ttg tgg ctc gcg gcg cag ttt ggc gac gcg cag aac atc ctg atc      192
Phe Leu Trp Leu Ala Ala Gln Phe Gly Asp Ala Gln Asn Ile Leu Ile         
    50                  55                  60                          

gac cga gcg tcg ccg ggc gtc gcg tac aag gga ttg atg gcg aac gag      240
Asp Arg Ala Ser Pro Gly Val Ala Tyr Lys Gly Leu Met Ala Asn Glu         
65                  70                  75                  80          

gtc gcg ctg tac gtt ctc ggt aag gtt tta cac ttt ggt ctt ctc ctc      288
Val Ala Leu Tyr Val Leu Gly Lys Val Leu His Phe Gly Leu Leu Leu         
                85                  90                  95              

ggc gtt cct gcg tac ttg cac gga ttg tcc aac gcg atc gtt cca ttc      336
Gly Val Pro Ala Tyr Leu His Gly Leu Ser Asn Ala Ile Val Pro Phe         
            100                 105                 110                 

ttg gcg tac ggc gca ttc ggc tcc ttc gtc ctg tgc tgg ttc ttc atc      384
Leu Ala Tyr Gly Ala Phe Gly Ser Phe Val Leu Cys Trp Phe Phe Ile         
        115                 120                 125                     

gtc agc cat aac ctc gaa gcg ctg aca ccc gtt aac ctt aac aag tcc      432
Val Ser His Asn Leu Glu Ala Leu Thr Pro Val Asn Leu Asn Lys Ser         
    130                 135                 140                         

acg aag aac gac tgg ggg gcg tgg cag atc gag aca tcg gcg tct tgg      480
Thr Lys Asn Asp Trp Gly Ala Trp Gln Ile Glu Thr Ser Ala Ser Trp         
145                 150                 155                 160         

ggc aac gcg ttc tgg agc ttc ttc tct gga ggt ctg aac ctg caa atc      528
Gly Asn Ala Phe Trp Ser Phe Phe Ser Gly Gly Leu Asn Leu Gln Ile         
                165                 170                 175             

gag cac cac ctc ttc ccg ggc atg gcg cac aac ctg tac ccg aag atg      576
Glu His His Leu Phe Pro Gly Met Ala His Asn Leu Tyr Pro Lys Met         
            180                 185                 190                 

gtg ccg atc atc aag gac gag tgt gcg aaa gcg ggc gtt cgc tac acc      624
Val Pro Ile Ile Lys Asp Glu Cys Ala Lys Ala Gly Val Arg Tyr Thr         
        195                 200                 205                     

ggt tac ggt ggc tac acc ggc ctg ctc ccg atc acc cgc gac atg ttc      672
Gly Tyr Gly Gly Tyr Thr Gly Leu Leu Pro Ile Thr Arg Asp Met Phe         
    210                 215                 220                         

tcc tac ctc cat aag tgt ggc cga acg gcg aaa cta gcc taa              714
Ser Tyr Leu His Lys Cys Gly Arg Thr Ala Lys Leu Ala                     
225                 230                 235                             


<210>  28

<211>  237

<212>  PRT

<213>  Ostreococcus tauri



<400>  28

Met Val Ser His His Ser Tyr Cys Asn Asp Ala Asp Leu Asp Gln Asp 
1               5                   10                  15      

Val Tyr Thr Ala Leu Pro Leu Leu Arg Leu Asp Pro Ser Gln Glu Leu 
            20                  25                  30          

Lys Trp Phe His Arg Tyr Gln Ala Phe Tyr Ala Pro Leu Met Trp Pro 
        35                  40                  45              

Phe Leu Trp Leu Ala Ala Gln Phe Gly Asp Ala Gln Asn Ile Leu Ile 
    50                  55                  60                  

Asp Arg Ala Ser Pro Gly Val Ala Tyr Lys Gly Leu Met Ala Asn Glu 
65                  70                  75                  80  

Val Ala Leu Tyr Val Leu Gly Lys Val Leu His Phe Gly Leu Leu Leu 
                85                  90                  95      

Gly Val Pro Ala Tyr Leu His Gly Leu Ser Asn Ala Ile Val Pro Phe 
            100                 105                 110         

Leu Ala Tyr Gly Ala Phe Gly Ser Phe Val Leu Cys Trp Phe Phe Ile 
        115                 120                 125             

Val Ser His Asn Leu Glu Ala Leu Thr Pro Val Asn Leu Asn Lys Ser 
    130                 135                 140                 

Thr Lys Asn Asp Trp Gly Ala Trp Gln Ile Glu Thr Ser Ala Ser Trp 
145                 150                 155                 160 

Gly Asn Ala Phe Trp Ser Phe Phe Ser Gly Gly Leu Asn Leu Gln Ile 
                165                 170                 175     

Glu His His Leu Phe Pro Gly Met Ala His Asn Leu Tyr Pro Lys Met 
            180                 185                 190         

Val Pro Ile Ile Lys Asp Glu Cys Ala Lys Ala Gly Val Arg Tyr Thr 
        195                 200                 205             

Gly Tyr Gly Gly Tyr Thr Gly Leu Leu Pro Ile Thr Arg Asp Met Phe 
    210                 215                 220                 

Ser Tyr Leu His Lys Cys Gly Arg Thr Ala Lys Leu Ala 
225                 230                 235         


<210>  29

<211>  1371

<212>  DNA

<213>  Ostreococcus tauri



<220>

<221>  CDS

<222>  (1)..(1371)

<223>  Delta-6-Desaturase



<400>  29
atg tgc gtg gag acg gaa aat aac gat ggg atc ccc acg gtg gag atc       48
Met Cys Val Glu Thr Glu Asn Asn Asp Gly Ile Pro Thr Val Glu Ile         
1               5                   10                  15              


gcg ttc gac ggt gag cgc gag cgg gcg gag gca aac gtg aag ctg tcc       96
Ala Phe Asp Gly Glu Arg Glu Arg Ala Glu Ala Asn Val Lys Leu Ser         
            20                  25                  30                  

gcg gag aag atg gag ccg gcg gcg ctg gcg aag acg ttc gcg agg cgg      144
Ala Glu Lys Met Glu Pro Ala Ala Leu Ala Lys Thr Phe Ala Arg Arg         
        35                  40                  45                      

tac gtc gtg atc gag ggg gtg gag tac gat gtg acg gat ttt aag cac      192
Tyr Val Val Ile Glu Gly Val Glu Tyr Asp Val Thr Asp Phe Lys His         
    50                  55                  60                          

ccg gga gga acg gtt att ttc tat gcg ttg tca aac acc ggg gcg gac      240
Pro Gly Gly Thr Val Ile Phe Tyr Ala Leu Ser Asn Thr Gly Ala Asp         
65                  70                  75                  80          

gcg acg gaa gcg ttc aag gag ttt cat cat cgg tcg aga aag gcg agg      288
Ala Thr Glu Ala Phe Lys Glu Phe His His Arg Ser Arg Lys Ala Arg         
                85                  90                  95              

aaa gcc ttg gcg gcg ctc ccg tct cga ccg gcc aag acg gcc aag gtg      336
Lys Ala Leu Ala Ala Leu Pro Ser Arg Pro Ala Lys Thr Ala Lys Val         
            100                 105                 110                 

gac gac gcg gag atg ctc caa gat ttc gcc aag tgg cgg aaa gaa ttg      384
Asp Asp Ala Glu Met Leu Gln Asp Phe Ala Lys Trp Arg Lys Glu Leu         
        115                 120                 125                     

gag aga gat gga ttc ttc aag ccc tct ccg gcg cac gtg gcg tat cgc      432
Glu Arg Asp Gly Phe Phe Lys Pro Ser Pro Ala His Val Ala Tyr Arg         
    130                 135                 140                         


ttc gcc gag ctc gcg gcg atg tac gct ctc ggg acg tac ctg atg tac      480
Phe Ala Glu Leu Ala Ala Met Tyr Ala Leu Gly Thr Tyr Leu Met Tyr         
145                 150                 155                 160         

gct cga tac gtc gtc tcc tcg gtg ctc gtg tac gct tgc ttt ttc ggc      528
Ala Arg Tyr Val Val Ser Ser Val Leu Val Tyr Ala Cys Phe Phe Gly         
                165                 170                 175             

gcc cga tgc ggt tgg gtg cag cac gag ggc gga cac agc tcg ctg acg      576
Ala Arg Cys Gly Trp Val Gln His Glu Gly Gly His Ser Ser Leu Thr         
            180                 185                 190                 

ggc aac att tgg tgg gac aag cgc atc cag gcc ttc aca gcc ggg ttc      624
Gly Asn Ile Trp Trp Asp Lys Arg Ile Gln Ala Phe Thr Ala Gly Phe         
        195                 200                 205                     

ggt ctc gcc ggt agc ggc gac atg tgg aac tcg atg cac aac aag cat      672
Gly Leu Ala Gly Ser Gly Asp Met Trp Asn Ser Met His Asn Lys His         
    210                 215                 220                         

cac gcg acg cct caa aag gtt cgt cac gac atg gat ctg gac acc acc      720
His Ala Thr Pro Gln Lys Val Arg His Asp Met Asp Leu Asp Thr Thr         
225                 230                 235                 240         

ccc gcg gtg gcg ttc ttc aac acc gcg gtg gaa gac aat cgt ccc cgt      768
Pro Ala Val Ala Phe Phe Asn Thr Ala Val Glu Asp Asn Arg Pro Arg         
                245                 250                 255             

ggc ttt agc aag tac tgg ttg cgc ctt cag gcg tgg acc ttc atc ccc      816
Gly Phe Ser Lys Tyr Trp Leu Arg Leu Gln Ala Trp Thr Phe Ile Pro         
            260                 265                 270                 


gtg acg tcc ggc ttg gtg ctc ctt ttc tgg atg ttt ttc ctc cac ccc      864
Val Thr Ser Gly Leu Val Leu Leu Phe Trp Met Phe Phe Leu His Pro         
        275                 280                 285                     

tcc aag gct ttg aag ggt ggc aag tac gaa gag ttg gtg tgg atg ctc      912
Ser Lys Ala Leu Lys Gly Gly Lys Tyr Glu Glu Leu Val Trp Met Leu         
    290                 295                 300                         

gcc gcg cac gtc atc cgc acg tgg acg atc aag gcg gtg acc gga ttc      960
Ala Ala His Val Ile Arg Thr Trp Thr Ile Lys Ala Val Thr Gly Phe         
305                 310                 315                 320         

acc gcg atg cag tcc tac ggc tta ttt ttg gcg acg agc tgg gtg agc     1008
Thr Ala Met Gln Ser Tyr Gly Leu Phe Leu Ala Thr Ser Trp Val Ser         
                325                 330                 335             

ggc tgc tat ctg ttt gca cac ttc tcc acg tcg cac acg cac ctg gat     1056
Gly Cys Tyr Leu Phe Ala His Phe Ser Thr Ser His Thr His Leu Asp         
            340                 345                 350                 

gtg gtg ccc gcg gac gag cat ctc tcc tgg gtt cga tac gcc gtc gat     1104
Val Val Pro Ala Asp Glu His Leu Ser Trp Val Arg Tyr Ala Val Asp         
        355                 360                 365                     

cac acg atc gac atc gat ccg agt caa ggt tgg gtg aac tgg ttg atg     1152
His Thr Ile Asp Ile Asp Pro Ser Gln Gly Trp Val Asn Trp Leu Met         
    370                 375                 380                         

ggc tac ctc aac tgc caa gtc atc cac cac ctc ttt ccg agc atg ccg     1200
Gly Tyr Leu Asn Cys Gln Val Ile His His Leu Phe Pro Ser Met Pro         
385                 390                 395                 400         


cag ttc cgc cag ccc gag gta tct cgc cgc ttc gtc gcc ttt gcg aaa     1248
Gln Phe Arg Gln Pro Glu Val Ser Arg Arg Phe Val Ala Phe Ala Lys         
                405                 410                 415             


aag tgg aac ctc aac tac aag gtc atg acc tac gcc ggt gcg tgg aag     1296
Lys Trp Asn Leu Asn Tyr Lys Val Met Thr Tyr Ala Gly Ala Trp Lys         
            420                 425                 430                 

gca acg ctc gga aac ctc gac aac gtg ggt aag cac tac tac gtg cac     1344
Ala Thr Leu Gly Asn Leu Asp Asn Val Gly Lys His Tyr Tyr Val His         
        435                 440                 445                     

ggc caa cac tcc gga aag acg gcg taa                                 1371
Gly Gln His Ser Gly Lys Thr Ala                                         
    450                 455                                             


<210>  30

<211>  456

<212>  PRT

<213>  Ostreococcus tauri



<400>  30

Met Cys Val Glu Thr Glu Asn Asn Asp Gly Ile Pro Thr Val Glu Ile 
1               5                   10                  15      

Ala Phe Asp Gly Glu Arg Glu Arg Ala Glu Ala Asn Val Lys Leu Ser 
            20                  25                  30          

Ala Glu Lys Met Glu Pro Ala Ala Leu Ala Lys Thr Phe Ala Arg Arg 
        35                  40                  45              

Tyr Val Val Ile Glu Gly Val Glu Tyr Asp Val Thr Asp Phe Lys His 
    50                  55                  60                  

Pro Gly Gly Thr Val Ile Phe Tyr Ala Leu Ser Asn Thr Gly Ala Asp 
65                  70                  75                  80  

Ala Thr Glu Ala Phe Lys Glu Phe His His Arg Ser Arg Lys Ala Arg 
                85                  90                  95      

Lys Ala Leu Ala Ala Leu Pro Ser Arg Pro Ala Lys Thr Ala Lys Val 
            100                 105                 110         

Asp Asp Ala Glu Met Leu Gln Asp Phe Ala Lys Trp Arg Lys Glu Leu 
        115                 120                 125             

Glu Arg Asp Gly Phe Phe Lys Pro Ser Pro Ala His Val Ala Tyr Arg 
    130                 135                 140                 

Phe Ala Glu Leu Ala Ala Met Tyr Ala Leu Gly Thr Tyr Leu Met Tyr 
145                 150                 155                 160 

Ala Arg Tyr Val Val Ser Ser Val Leu Val Tyr Ala Cys Phe Phe Gly 
                165                 170                 175     

Ala Arg Cys Gly Trp Val Gln His Glu Gly Gly His Ser Ser Leu Thr 
            180                 185                 190         

Gly Asn Ile Trp Trp Asp Lys Arg Ile Gln Ala Phe Thr Ala Gly Phe 
        195                 200                 205             

Gly Leu Ala Gly Ser Gly Asp Met Trp Asn Ser Met His Asn Lys His 

    210                 215                 220                 

His Ala Thr Pro Gln Lys Val Arg His Asp Met Asp Leu Asp Thr Thr 
225                 230                 235                 240 

Pro Ala Val Ala Phe Phe Asn Thr Ala Val Glu Asp Asn Arg Pro Arg 
                245                 250                 255     

Gly Phe Ser Lys Tyr Trp Leu Arg Leu Gln Ala Trp Thr Phe Ile Pro 
            260                 265                 270         

Val Thr Ser Gly Leu Val Leu Leu Phe Trp Met Phe Phe Leu His Pro 
        275                 280                 285             

Ser Lys Ala Leu Lys Gly Gly Lys Tyr Glu Glu Leu Val Trp Met Leu 
    290                 295                 300                 

Ala Ala His Val Ile Arg Thr Trp Thr Ile Lys Ala Val Thr Gly Phe 
305                 310                 315                 320 

Thr Ala Met Gln Ser Tyr Gly Leu Phe Leu Ala Thr Ser Trp Val Ser 
                325                 330                 335     

Gly Cys Tyr Leu Phe Ala His Phe Ser Thr Ser His Thr His Leu Asp 
            340                 345                 350         

Val Val Pro Ala Asp Glu His Leu Ser Trp Val Arg Tyr Ala Val Asp 
        355                 360                 365             

His Thr Ile Asp Ile Asp Pro Ser Gln Gly Trp Val Asn Trp Leu Met 
    370                 375                 380                 

Gly Tyr Leu Asn Cys Gln Val Ile His His Leu Phe Pro Ser Met Pro 
385                 390                 395                 400 

Gln Phe Arg Gln Pro Glu Val Ser Arg Arg Phe Val Ala Phe Ala Lys 
                405                 410                 415     

Lys Trp Asn Leu Asn Tyr Lys Val Met Thr Tyr Ala Gly Ala Trp Lys 
            420                 425                 430         

Ala Thr Leu Gly Asn Leu Asp Asn Val Gly Lys His Tyr Tyr Val His 
        435                 440                 445             

Gly Gln His Ser Gly Lys Thr Ala 
    450                 455     


<210>  31

<211>  1086

<212>  DNA

<213>  Ostreococcus tauri



<220>

<221>  CDS

<222>  (1)..(1086)

<223>  Delta-12-Desaturase



<400>  31
atg cag gag ggg gtg cga aac att ccg aac gag tgc ttt gag acg gga       48
Met Gln Glu Gly Val Arg Asn Ile Pro Asn Glu Cys Phe Glu Thr Gly         
1               5                   10                  15              

cat ctt gaa aga ccc tgg cgt tcc ggc cgg tgt ggg cgc gat ccc ggt       96
His Leu Glu Arg Pro Trp Arg Ser Gly Arg Cys Gly Arg Asp Pro Gly         
            20                  25                  30                  

tcg aat tgg ggc gct ggc ttc cgc ttt ttt tcg ctc aag ggg ttt tgg      144
Ser Asn Trp Gly Ala Gly Phe Arg Phe Phe Ser Leu Lys Gly Phe Trp         
        35                  40                  45                      

tgg ccg gcg tgg tgg gcg tac gcg ttc gtg acg ggg acg gcg gcc act      192
Trp Pro Ala Trp Trp Ala Tyr Ala Phe Val Thr Gly Thr Ala Ala Thr         
    50                  55                  60                          

ggg tgt tgg gtc gcc gcg cac gag tgc ggg cac ggc gcg ttc agc gat      240
Gly Cys Trp Val Ala Ala His Glu Cys Gly His Gly Ala Phe Ser Asp         
65                  70                  75                  80          

aac aag acg ttg caa gat gcg gtt gga tac gtg ttg cac tcg ttg ctc      288
Asn Lys Thr Leu Gln Asp Ala Val Gly Tyr Val Leu His Ser Leu Leu         
                85                  90                  95              



ttg gtg ccg tac ttt tct tgg cag cga tca cac gcg gtg cat cac tcg      336
Leu Val Pro Tyr Phe Ser Trp Gln Arg Ser His Ala Val His His Ser         
            100                 105                 110                 

agg acg aat cac gtt ctt gag ggc gag acg cac gtg ccg gcg cgc ttg      384
Arg Thr Asn His Val Leu Glu Gly Glu Thr His Val Pro Ala Arg Leu         
        115                 120                 125                     

ggg acg gaa gac gcc aac gtc gtg ttc aag ctt cgc gaa ttg atc ggt      432
Gly Thr Glu Asp Ala Asn Val Val Phe Lys Leu Arg Glu Leu Ile Gly         
    130                 135                 140                         

gaa ggg ccg ttc acg ttt ttc aac ctc gtc ggc gtc ttc gcg ctc gga      480
Glu Gly Pro Phe Thr Phe Phe Asn Leu Val Gly Val Phe Ala Leu Gly         
145                 150                 155                 160         

tgg ccg att tac ttg ctc acc ggc gcg agc ggc gga ccg gtg cgc ggt      528
Trp Pro Ile Tyr Leu Leu Thr Gly Ala Ser Gly Gly Pro Val Arg Gly         
                165                 170                 175             

aac acg aac cac ttc tta ccc ttc atg ggc gag aaa ggt aag cac gcg      576
Asn Thr Asn His Phe Leu Pro Phe Met Gly Glu Lys Gly Lys His Ala         
            180                 185                 190                 

ctg ttc ccg ggt aag tgg gcg aag aag gtg tgg cag tct gac atc ggc      624
Leu Phe Pro Gly Lys Trp Ala Lys Lys Val Trp Gln Ser Asp Ile Gly         
        195                 200                 205                     

gtt gtt gcc gtc ctg ggc gcg ctc gcg gct tgg gcg gcg cac agc ggg      672
Val Val Ala Val Leu Gly Ala Leu Ala Ala Trp Ala Ala His Ser Gly         
    210                 215                 220                         

att gcc aca gtg atg gca ctc tac gtc ggc ccg tac atg gtg acc aac      720
Ile Ala Thr Val Met Ala Leu Tyr Val Gly Pro Tyr Met Val Thr Asn         
225                 230                 235                 240         

ttt tgg ctc gtc ttg tac acg tgg tta cag cac acc gac gtt gac gtg      768
Phe Trp Leu Val Leu Tyr Thr Trp Leu Gln His Thr Asp Val Asp Val         
                245                 250                 255             

ccg cac ttc gag ggc gac gat tgg aac ttg gtc aag ggg gca ttc atg      816
Pro His Phe Glu Gly Asp Asp Trp Asn Leu Val Lys Gly Ala Phe Met         
            260                 265                 270                 

acg atc gat cgc ccg tac ggc cca gtt ttt gat ttc ttg cac cac cgc      864
Thr Ile Asp Arg Pro Tyr Gly Pro Val Phe Asp Phe Leu His His Arg         
        275                 280                 285                     

atc ggc agc acg cac gtc gcg cac cac atc aac aca cca ttc ccg cat      912
Ile Gly Ser Thr His Val Ala His His Ile Asn Thr Pro Phe Pro His         
    290                 295                 300                         

tac aag gct caa atg gcg acg gat gcg cta aag gag gcg tat ccc gac      960
Tyr Lys Ala Gln Met Ala Thr Asp Ala Leu Lys Glu Ala Tyr Pro Asp         
305                 310                 315                 320         

ctc tac ctt tac gat cca act ccg atc gcg acc gct acg tgg cgc gtg     1008
Leu Tyr Leu Tyr Asp Pro Thr Pro Ile Ala Thr Ala Thr Trp Arg Val         
                325                 330                 335             

ggg agc aag tgc atc gcc gtc gtg aag aag gga gac gaa tgg gtg ttc     1056
Gly Ser Lys Cys Ile Ala Val Val Lys Lys Gly Asp Glu Trp Val Phe         
            340                 345                 350                 

acg gat aag caa ctc ccg gtc gcg gcg tga                             1086
Thr Asp Lys Gln Leu Pro Val Ala Ala                                     
        355                 360                                         


<210>  32

<211>  361

<212>  PRT

<213>  Ostreococcus tauri



<400>  32

Met Gln Glu Gly Val Arg Asn Ile Pro Asn Glu Cys Phe Glu Thr Gly 
1               5                   10                  15      

His Leu Glu Arg Pro Trp Arg Ser Gly Arg Cys Gly Arg Asp Pro Gly 
            20                  25                  30          

Ser Asn Trp Gly Ala Gly Phe Arg Phe Phe Ser Leu Lys Gly Phe Trp 
        35                  40                  45              

Trp Pro Ala Trp Trp Ala Tyr Ala Phe Val Thr Gly Thr Ala Ala Thr 
    50                  55                  60                  

Gly Cys Trp Val Ala Ala His Glu Cys Gly His Gly Ala Phe Ser Asp 
65                  70                  75                  80  

Asn Lys Thr Leu Gln Asp Ala Val Gly Tyr Val Leu His Ser Leu Leu 
                85                  90                  95      

Leu Val Pro Tyr Phe Ser Trp Gln Arg Ser His Ala Val His His Ser 
            100                 105                 110         

Arg Thr Asn His Val Leu Glu Gly Glu Thr His Val Pro Ala Arg Leu 
        115                 120                 125             

Gly Thr Glu Asp Ala Asn Val Val Phe Lys Leu Arg Glu Leu Ile Gly 
    130                 135                 140                 

Glu Gly Pro Phe Thr Phe Phe Asn Leu Val Gly Val Phe Ala Leu Gly 
145                 150                 155                 160 

Trp Pro Ile Tyr Leu Leu Thr Gly Ala Ser Gly Gly Pro Val Arg Gly 
                165                 170                 175     

Asn Thr Asn His Phe Leu Pro Phe Met Gly Glu Lys Gly Lys His Ala 
            180                 185                 190         

Leu Phe Pro Gly Lys Trp Ala Lys Lys Val Trp Gln Ser Asp Ile Gly 
        195                 200                 205             

Val Val Ala Val Leu Gly Ala Leu Ala Ala Trp Ala Ala His Ser Gly 
    210                 215                 220                 

Ile Ala Thr Val Met Ala Leu Tyr Val Gly Pro Tyr Met Val Thr Asn 
225                 230                 235                 240 

Phe Trp Leu Val Leu Tyr Thr Trp Leu Gln His Thr Asp Val Asp Val 
                245                 250                 255     

Pro His Phe Glu Gly Asp Asp Trp Asn Leu Val Lys Gly Ala Phe Met 
            260                 265                 270         

Thr Ile Asp Arg Pro Tyr Gly Pro Val Phe Asp Phe Leu His His Arg 
        275                 280                 285             

Ile Gly Ser Thr His Val Ala His His Ile Asn Thr Pro Phe Pro His 
    290                 295                 300                 

Tyr Lys Ala Gln Met Ala Thr Asp Ala Leu Lys Glu Ala Tyr Pro Asp 
305                 310                 315                 320 

Leu Tyr Leu Tyr Asp Pro Thr Pro Ile Ala Thr Ala Thr Trp Arg Val 
                325                 330                 335     

Gly Ser Lys Cys Ile Ala Val Val Lys Lys Gly Asp Glu Trp Val Phe 
            340                 345                 350         

Thr Asp Lys Gln Leu Pro Val Ala Ala 
355	360     
        

<210>  33

<211>  1077

<212>  DNA

<213>  Thalassiosira pseudonana



<220>

<221>  CDS

<222>  (1)..(1077)

<223>  Delta-5-Elongase



<400>  33
atg tgc tca tca ccg ccg tca caa tcc aaa aca aca tcc ctc cta gca       48
Met Cys Ser Ser Pro Pro Ser Gln Ser Lys Thr Thr Ser Leu Leu Ala         
1               5                   10                  15              

cgg tac acc acc gcc gcc ctc ctc ctc ctc acc ctc aca aca tgg tgc       96
Arg Tyr Thr Thr Ala Ala Leu Leu Leu Leu Thr Leu Thr Thr Trp Cys         
            20                  25                  30                  

cac ttc gcc ttc cca gcc gcc acc gcc aca ccc ggc ctc acc gcc gaa      144
His Phe Ala Phe Pro Ala Ala Thr Ala Thr Pro Gly Leu Thr Ala Glu         
        35                  40                  45                      

atg cac tcc tac aaa gtc cca ctc ggt ctc acc gta ttc tac ctg ctg      192
Met His Ser Tyr Lys Val Pro Leu Gly Leu Thr Val Phe Tyr Leu Leu         
    50                  55                  60                          

agt cta ccg tca cta aag tac gtt acg gac aac tac ctt gcc aaa aag      240
Ser Leu Pro Ser Leu Lys Tyr Val Thr Asp Asn Tyr Leu Ala Lys Lys         
65                  70                  75                  80          

tat gat atg aag tca ctc cta acg gaa tca atg gtg ttg tac aat gtg      288
Tyr Asp Met Lys Ser Leu Leu Thr Glu Ser Met Val Leu Tyr Asn Val         
                85                  90                  95              

gcg caa gtg ctg ctc aat ggg tgg acg gtg tat gcg att gtg gat gcg      336
Ala Gln Val Leu Leu Asn Gly Trp Thr Val Tyr Ala Ile Val Asp Ala         
            100                 105                 110                 

gtg atg aat aga gac cat ccg ttt att gga agt aga agt ttg gtt ggg      384
Val Met Asn Arg Asp His Pro Phe Ile Gly Ser Arg Ser Leu Val Gly         
        115                 120                 125                     

gcg gcg ttg cat agt ggg agc tcg tat gcg gtg tgg gtt cat tat tgt      432
Ala Ala Leu His Ser Gly Ser Ser Tyr Ala Val Trp Val His Tyr Cys         
    130                 135                 140                         

gat aag tat ttg gag ttc ttt gat acg tat ttt atg gtg ttg agg ggg      480
Asp Lys Tyr Leu Glu Phe Phe Asp Thr Tyr Phe Met Val Leu Arg Gly         
145                 150                 155                 160         

aaa atg gac cag gtc tcc ttc ctc cac atc tac cac cac acg acc ata      528
Lys Met Asp Gln Val Ser Phe Leu His Ile Tyr His His Thr Thr Ile         
                165                 170                 175             

gcg tgg gca tgg tgg atc gcc ctc cgc ttc tcc ccc ggt gga gac att      576
Ala Trp Ala Trp Trp Ile Ala Leu Arg Phe Ser Pro Gly Gly Asp Ile         
            180                 185                 190                 

tac ttc ggg gca ctc ctc aac tcc atc atc cac gtc ctc atg tat tcc      624
Tyr Phe Gly Ala Leu Leu Asn Ser Ile Ile His Val Leu Met Tyr Ser         
        195                 200                 205                     

tac tac gcc ctt gcc cta ctc aag gtc agt tgt cca tgg aaa cga tac      672
Tyr Tyr Ala Leu Ala Leu Leu Lys Val Ser Cys Pro Trp Lys Arg Tyr         
    210                 215                 220                         

ctg act caa gct caa tta ttg caa ttc aca agt gtg gtg gtt tat acg      720
Leu Thr Gln Ala Gln Leu Leu Gln Phe Thr Ser Val Val Val Tyr Thr         
225                 230                 235                 240         

ggg tgt acg ggt tat act cat tac tat cat acg aag cat gga gcg gat      768
Gly Cys Thr Gly Tyr Thr His Tyr Tyr His Thr Lys His Gly Ala Asp         
                245                 250                 255             

gag aca cag cct agt tta gga acg tat tat ttc tgt tgt gga gtg cag      816
Glu Thr Gln Pro Ser Leu Gly Thr Tyr Tyr Phe Cys Cys Gly Val Gln         
            260                 265                 270                 

gtg ttt gag atg gtt agt ttg ttt gta ctc ttt tcc atc ttt tat aaa      864
Val Phe Glu Met Val Ser Leu Phe Val Leu Phe Ser Ile Phe Tyr Lys         
        275                 280                 285                     

cga tcc tat tcg aag aag aac aag tca gga gga aag gat agc aag aag      912
Arg Ser Tyr Ser Lys Lys Asn Lys Ser Gly Gly Lys Asp Ser Lys Lys         
    290                 295                 300                         

aat gat gat ggg aat aat gag gat caa tgt cac aag gct atg aag gat      960
Asn Asp Asp Gly Asn Asn Glu Asp Gln Cys His Lys Ala Met Lys Asp         
305                 310                 315                 320         

ata tcg gag ggt gcg aag gag gtt gtg ggg cat gca gcg aag gat gct     1008
Ile Ser Glu Gly Ala Lys Glu Val Val Gly His Ala Ala Lys Asp Ala         
                325                 330                 335             

gga aag ttg gtg gct acg gcg agt aag gct gta aag agg aag gga act     1056
Gly Lys Leu Val Ala Thr Ala Ser Lys Ala Val Lys Arg Lys Gly Thr         
            340                 345                 350                 

cgt gtt act ggt gcc atg tag                                         1077
Arg Val Thr Gly Ala Met                                                 
        355                                                             


<210>  34

<211>  358

<212>  PRT

<213>  Thalassiosira pseudonana



<400>  34

Met Cys Ser Ser Pro Pro Ser Gln Ser Lys Thr Thr Ser Leu Leu Ala 
1               5                   10                  15      


Arg Tyr Thr Thr Ala Ala Leu Leu Leu Leu Thr Leu Thr Thr Trp Cys 
            20                  25                  30          


His Phe Ala Phe Pro Ala Ala Thr Ala Thr Pro Gly Leu Thr Ala Glu 
        35                  40                  45              


Met His Ser Tyr Lys Val Pro Leu Gly Leu Thr Val Phe Tyr Leu Leu 
    50                  55                  60                  


Ser Leu Pro Ser Leu Lys Tyr Val Thr Asp Asn Tyr Leu Ala Lys Lys 
65                  70                  75                  80  


Tyr Asp Met Lys Ser Leu Leu Thr Glu Ser Met Val Leu Tyr Asn Val 
                85                  90                  95      


Ala Gln Val Leu Leu Asn Gly Trp Thr Val Tyr Ala Ile Val Asp Ala 
            100                 105                 110         


Val Met Asn Arg Asp His Pro Phe Ile Gly Ser Arg Ser Leu Val Gly 
        115                 120                 125             


Ala Ala Leu His Ser Gly Ser Ser Tyr Ala Val Trp Val His Tyr Cys 
    130                 135                 140                 


Asp Lys Tyr Leu Glu Phe Phe Asp Thr Tyr Phe Met Val Leu Arg Gly 
145                 150                 155                 160 


Lys Met Asp Gln Val Ser Phe Leu His Ile Tyr His His Thr Thr Ile 
                165                 170                 175     


Ala Trp Ala Trp Trp Ile Ala Leu Arg Phe Ser Pro Gly Gly Asp Ile 
            180                 185                 190         


Tyr Phe Gly Ala Leu Leu Asn Ser Ile Ile His Val Leu Met Tyr Ser 
        195                 200                 205             


Tyr Tyr Ala Leu Ala Leu Leu Lys Val Ser Cys Pro Trp Lys Arg Tyr 
    210                 215                 220                 


Leu Thr Gln Ala Gln Leu Leu Gln Phe Thr Ser Val Val Val Tyr Thr 
225                 230                 235                 240 


Gly Cys Thr Gly Tyr Thr His Tyr Tyr His Thr Lys His Gly Ala Asp 
                245                 250                 255     


Glu Thr Gln Pro Ser Leu Gly Thr Tyr Tyr Phe Cys Cys Gly Val Gln 
            260                 265                 270         


Val Phe Glu Met Val Ser Leu Phe Val Leu Phe Ser Ile Phe Tyr Lys 
        275                 280                 285             


Arg Ser Tyr Ser Lys Lys Asn Lys Ser Gly Gly Lys Asp Ser Lys Lys 
    290                 295                 300                 


Asn Asp Asp Gly Asn Asn Glu Asp Gln Cys His Lys Ala Met Lys Asp 
305                 310                 315                 320 


Ile Ser Glu Gly Ala Lys Glu Val Val Gly His Ala Ala Lys Asp Ala 
                325                 330                 335     


Gly Lys Leu Val Ala Thr Ala Ser Lys Ala Val Lys Arg Lys Gly Thr 
            340                 345                 350         


Arg Val Thr Gly Ala Met 
        355             


<210>  35

<211>  819

<212>  DNA

<213>  Thalassiosira pseudonana



<220>

<221>  CDS

<222>  (1)..(819)

<223>  Delta-5-Elongase



<400>  35
atg gac gcc tac aac gct gca atg gat aag atc ggt gcc gcc atc atc       48
Met Asp Ala Tyr Asn Ala Ala Met Asp Lys Ile Gly Ala Ala Ile Ile         
1               5                   10                  15              

gat tgg tct gat ccc gat gga aag ttc cgt gcc gat aga gag gac tgg       96
Asp Trp Ser Asp Pro Asp Gly Lys Phe Arg Ala Asp Arg Glu Asp Trp         
            20                  25                  30                  

tgg ctc tgc gac ttc cgt agc gcc atc acc atc gcc ctc atc tac atc      144
Trp Leu Cys Asp Phe Arg Ser Ala Ile Thr Ile Ala Leu Ile Tyr Ile         
        35                  40                  45                      

gcc ttc gtc atc ctc ggt tcc gcc gtc atg caa tcc ctc ccc gca atg      192
Ala Phe Val Ile Leu Gly Ser Ala Val Met Gln Ser Leu Pro Ala Met         
    50                  55                  60                          

gat ccc tac ccc atc aaa ttc ctc tac aac gtc tcc caa atc ttc ctt      240
Asp Pro Tyr Pro Ile Lys Phe Leu Tyr Asn Val Ser Gln Ile Phe Leu         
65                  70                  75                  80          

tgt gcc tac atg act gtc gag gcg gga ttt ttg gcc tac cgc aat gga      288
Cys Ala Tyr Met Thr Val Glu Ala Gly Phe Leu Ala Tyr Arg Asn Gly         
                85                  90                  95              

tat acc gtc atg cct tgc aat cat ttc aat gtg aat gat cct ccc gtg      336
Tyr Thr Val Met Pro Cys Asn His Phe Asn Val Asn Asp Pro Pro Val         
            100                 105                 110                 

gcg aat ctt ctt tgg ttg ttt tat att tcc aag gtg tgg gac ttt tgg      384
Ala Asn Leu Leu Trp Leu Phe Tyr Ile Ser Lys Val Trp Asp Phe Trp         
        115                 120                 125                     

gat acc att ttc att gtg ttg ggg aag aag tgg cgt caa tta tct ttc      432
Asp Thr Ile Phe Ile Val Leu Gly Lys Lys Trp Arg Gln Leu Ser Phe         
    130                 135                 140                         

ttg cat gta tac cat cac acc acc atc ttt cta ttc tat tgg ctg aat      480
Leu His Val Tyr His His Thr Thr Ile Phe Leu Phe Tyr Trp Leu Asn         
145                 150                 155                 160         

gcc aat gtc ttg tac gat ggt gac atc ttc ctt acc atc ttg ctc aat      528
Ala Asn Val Leu Tyr Asp Gly Asp Ile Phe Leu Thr Ile Leu Leu Asn         
                165                 170                 175             

gga ttc atc cac acg gtg atg tac acg tat tac ttc atc tgt atg cat      576
Gly Phe Ile His Thr Val Met Tyr Thr Tyr Tyr Phe Ile Cys Met His         
            180                 185                 190                 

acc aaa gat tcc aag acg ggc aag agt ctt cct ata tgg tgg aag tcg      624
Thr Lys Asp Ser Lys Thr Gly Lys Ser Leu Pro Ile Trp Trp Lys Ser         
        195                 200                 205                     

agt ttg acg gcg ttt cag ttg ttg caa ttc act atc atg atg agt cag      672
Ser Leu Thr Ala Phe Gln Leu Leu Gln Phe Thr Ile Met Met Ser Gln         
    210                 215                 220                         

gct acc tac ctt gtc ttc cac ggg tgt gat aag gtg tcg ctt cgt atc      720
Ala Thr Tyr Leu Val Phe His Gly Cys Asp Lys Val Ser Leu Arg Ile         
225                 230                 235                 240         

acg att gtg tac ttt gtg tcc ctt ttg agt ttg ttc ttc ctt ttt gct      768
Thr Ile Val Tyr Phe Val Ser Leu Leu Ser Leu Phe Phe Leu Phe Ala         
                245                 250                 255             

cag ttc ttt gtg caa tca tac atg gca ccc aaa aag aag aag agt gct      816
Gln Phe Phe Val Gln Ser Tyr Met Ala Pro Lys Lys Lys Lys Ser Ala         
            260                 265                 270                 

tag                                                                  819


<210>  36

<211>  272

<212>  PRT

<213>  Thalassiosira pseudonana



<400>  36

Met Asp Ala Tyr Asn Ala Ala Met Asp Lys Ile Gly Ala Ala Ile Ile 
1               5                   10                  15      


Asp Trp Ser Asp Pro Asp Gly Lys Phe Arg Ala Asp Arg Glu Asp Trp 
            20                  25                  30          


Trp Leu Cys Asp Phe Arg Ser Ala Ile Thr Ile Ala Leu Ile Tyr Ile 
        35                  40                  45              


Ala Phe Val Ile Leu Gly Ser Ala Val Met Gln Ser Leu Pro Ala Met 
    50                  55                  60                  


Asp Pro Tyr Pro Ile Lys Phe Leu Tyr Asn Val Ser Gln Ile Phe Leu 
65                  70                  75                  80  


Cys Ala Tyr Met Thr Val Glu Ala Gly Phe Leu Ala Tyr Arg Asn Gly 
                85                  90                  95      


Tyr Thr Val Met Pro Cys Asn His Phe Asn Val Asn Asp Pro Pro Val 
            100                 105                 110         


Ala Asn Leu Leu Trp Leu Phe Tyr Ile Ser Lys Val Trp Asp Phe Trp 
        115                 120                 125             


Asp Thr Ile Phe Ile Val Leu Gly Lys Lys Trp Arg Gln Leu Ser Phe 
    130                 135                 140                 


Leu His Val Tyr His His Thr Thr Ile Phe Leu Phe Tyr Trp Leu Asn 
145                 150                 155                 160 


Ala Asn Val Leu Tyr Asp Gly Asp Ile Phe Leu Thr Ile Leu Leu Asn 
                165                 170                 175     


Gly Phe Ile His Thr Val Met Tyr Thr Tyr Tyr Phe Ile Cys Met His 
            180                 185                 190         


Thr Lys Asp Ser Lys Thr Gly Lys Ser Leu Pro Ile Trp Trp Lys Ser 
        195                 200                 205             


Ser Leu Thr Ala Phe Gln Leu Leu Gln Phe Thr Ile Met Met Ser Gln 
    210                 215                 220                 


Ala Thr Tyr Leu Val Phe His Gly Cys Asp Lys Val Ser Leu Arg Ile 
225                 230                 235                 240 


Thr Ile Val Tyr Phe Val Ser Leu Leu Ser Leu Phe Phe Leu Phe Ala 
                245                 250                 255     


Gln Phe Phe Val Gln Ser Tyr Met Ala Pro Lys Lys Lys Lys Ser Ala 
            260                 265                 270         



<210>  37

<211>  1455

<212>  DNA

<213>  Thalassiosira pseudonana



<220>

<221>  CDS

<222>  (1)..(1455)

<223>  Delta-6-Desaturase



<400>  37
atg gga aaa gga gga gac gca gcc gca gct acc aag cgt agt gga gca       48
Met Gly Lys Gly Gly Asp Ala Ala Ala Ala Thr Lys Arg Ser Gly Ala         
1               5                   10                  15              

ttg aaa ttg gcg gag aag ccg cag aag tac act tgg cag gag gtg aag       96
Leu Lys Leu Ala Glu Lys Pro Gln Lys Tyr Thr Trp Gln Glu Val Lys         
            20                  25                  30                  

aag cac atc acc ccc gac gat gcc tgg gta gtc cac caa aac aaa gtc      144
Lys His Ile Thr Pro Asp Asp Ala Trp Val Val His Gln Asn Lys Val         
        35                  40                  45                      

tac gac gtc tcc aac tgg tac gac cac ccc ggt gga gcc gtg gtg ttc      192
Tyr Asp Val Ser Asn Trp Tyr Asp His Pro Gly Gly Ala Val Val Phe         
    50                  55                  60                          

acc cac gcc gga gac gac atg acg gac atc ttc gcc gcc ttc cac gcc      240
Thr His Ala Gly Asp Asp Met Thr Asp Ile Phe Ala Ala Phe His Ala         
65                  70                  75                  80          

caa ggc tct cag gcc atg atg aag aag ttt tac att gga gat ttg att      288
Gln Gly Ser Gln Ala Met Met Lys Lys Phe Tyr Ile Gly Asp Leu Ile         
                85                  90                  95              

ccg gag agt gtg gag cat aag gat caa aga cag ttg gat ttc gag aag      336
Pro Glu Ser Val Glu His Lys Asp Gln Arg Gln Leu Asp Phe Glu Lys         
            100                 105                 110                 

gga tat cgt gat tta cgg gcc aag ctt gtc atg atg ggg atg ttc aag      384
Gly Tyr Arg Asp Leu Arg Ala Lys Leu Val Met Met Gly Met Phe Lys         
        115                 120                 125                     

tcg agt aag atg tat tat gca tac aag tgc tcg ttc aat atg tgc atg      432
Ser Ser Lys Met Tyr Tyr Ala Tyr Lys Cys Ser Phe Asn Met Cys Met         
    130                 135                 140                         

tgg ttg gtg gcg gtg gcc atg gtg tac tac tcg gac agt ttg gca atg      480
Trp Leu Val Ala Val Ala Met Val Tyr Tyr Ser Asp Ser Leu Ala Met         
145                 150                 155                 160         

cac att gga tcg gct ctc ttg ttg gga ttg ttc tgg cag cag tgt gga      528
His Ile Gly Ser Ala Leu Leu Leu Gly Leu Phe Trp Gln Gln Cys Gly         
                165                 170                 175             

tgg ctt gcg cac gac ttt ctt cac cac caa gtc ttt aag caa cga aag      576
Trp Leu Ala His Asp Phe Leu His His Gln Val Phe Lys Gln Arg Lys         
            180                 185                 190                 

tac gga gat ctc gtt ggc atc ttt tgg gga gat ctc atg cag ggg ttc      624
Tyr Gly Asp Leu Val Gly Ile Phe Trp Gly Asp Leu Met Gln Gly Phe         
        195                 200                 205                     

tcg atg cag tgg tgg aag aac aag cac aat ggc cac cat gct gtt ccc      672
Ser Met Gln Trp Trp Lys Asn Lys His Asn Gly His His Ala Val Pro         
    210                 215                 220                         

aac ttg cac aac tct tcc ttg gac agt cag gat ggt gat ccc gat att      720
Asn Leu His Asn Ser Ser Leu Asp Ser Gln Asp Gly Asp Pro Asp Ile         
225                 230                 235                 240         

gat acc atg cca ctc ctt gct tgg agt ctc aag cag gct cag agt ttc      768
Asp Thr Met Pro Leu Leu Ala Trp Ser Leu Lys Gln Ala Gln Ser Phe         
                245                 250                 255             

aga gag atc aat aag gga aag gac agt acc ttc gtc aag tac gct atc      816
Arg Glu Ile Asn Lys Gly Lys Asp Ser Thr Phe Val Lys Tyr Ala Ile         
            260                 265                 270                 

aaa ttc cag gca ttc aca tac ttc ccc atc ctc ctc ttg gct cgc atc      864
Lys Phe Gln Ala Phe Thr Tyr Phe Pro Ile Leu Leu Leu Ala Arg Ile         
        275                 280                 285                     

tct tgg ttg aat gaa tcc ttc aaa act gca ttc gga ctc gga gct gcc      912
Ser Trp Leu Asn Glu Ser Phe Lys Thr Ala Phe Gly Leu Gly Ala Ala         
    290                 295                 300                         

tcg gag aat gcc aag ttg gag ttg gag aag cgt gga ctt cag tac cca      960
Ser Glu Asn Ala Lys Leu Glu Leu Glu Lys Arg Gly Leu Gln Tyr Pro         
305                 310                 315                 320         

ctt ttg gag aag ctt gga atc acc ctt cat tac act tgg atg ttc gtc     1008
Leu Leu Glu Lys Leu Gly Ile Thr Leu His Tyr Thr Trp Met Phe Val         
                325                 330                 335             

ctc tct tcc gga ttt gga agg tgg tct ctt cca tat tcc atc atg tat     1056
Leu Ser Ser Gly Phe Gly Arg Trp Ser Leu Pro Tyr Ser Ile Met Tyr         
            340                 345                 350                 

ttc ttc act gcc aca tgc tcc tcg gga ctt ttc ctc gca ttg gtc ttt     1104
Phe Phe Thr Ala Thr Cys Ser Ser Gly Leu Phe Leu Ala Leu Val Phe         
        355                 360                 365                     

gga ttg gga cac aac ggt atg tca gtg tac gat gcc acc acc cga cct     1152
Gly Leu Gly His Asn Gly Met Ser Val Tyr Asp Ala Thr Thr Arg Pro         
    370                 375                 380                         

gac ttc tgg caa ctc caa gtc acc act aca cgt aac atc att ggt gga     1200
Asp Phe Trp Gln Leu Gln Val Thr Thr Thr Arg Asn Ile Ile Gly Gly         
385                 390                 395                 400         

cac ggc att ccc caa ttc ttt gtg gat tgg ttc tgc ggt gga ttg caa     1248
His Gly Ile Pro Gln Phe Phe Val Asp Trp Phe Cys Gly Gly Leu Gln         
                405                 410                 415             

tac caa gtg gat cac cac ctc ttc ccc atg atg cct aga aac aat atc     1296
Tyr Gln Val Asp His His Leu Phe Pro Met Met Pro Arg Asn Asn Ile         
            420                 425                 430                 

gcg aaa tgc cac aag ctt gtg gag tca ttc tgt aag gag tgg ggt gtg     1344
Ala Lys Cys His Lys Leu Val Glu Ser Phe Cys Lys Glu Trp Gly Val         
        435                 440                 445                     

aag tac cat gag gcc gat atg tgg gat ggt acc gtg gaa gtg ttg caa     1392
Lys Tyr His Glu Ala Asp Met Trp Asp Gly Thr Val Glu Val Leu Gln         
    450                 455                 460                         

cat ctc tcc aag gtg tcg gat gat ttc ctt gtg gag atg gtg aag gat     1440
His Leu Ser Lys Val Ser Asp Asp Phe Leu Val Glu Met Val Lys Asp         
465                 470                 475                 480         

ttc cct gcc atg taa                                                 1455
Phe Pro Ala Met                                                         
                                                                        


<210>  38

<211>  484

<212>  PRT

<213>  Thalassiosira pseudonana



<400>  38

Met Gly Lys Gly Gly Asp Ala Ala Ala Ala Thr Lys Arg Ser Gly Ala 
1               5                   10                  15      


Leu Lys Leu Ala Glu Lys Pro Gln Lys Tyr Thr Trp Gln Glu Val Lys 
            20                  25                  30          


Lys His Ile Thr Pro Asp Asp Ala Trp Val Val His Gln Asn Lys Val 
        35                  40                  45              


Tyr Asp Val Ser Asn Trp Tyr Asp His Pro Gly Gly Ala Val Val Phe 
    50                  55                  60                  


Thr His Ala Gly Asp Asp Met Thr Asp Ile Phe Ala Ala Phe His Ala 
65                  70                  75                  80  


Gln Gly Ser Gln Ala Met Met Lys Lys Phe Tyr Ile Gly Asp Leu Ile 
                85                  90                  95      


Pro Glu Ser Val Glu His Lys Asp Gln Arg Gln Leu Asp Phe Glu Lys 
            100                 105                 110         


Gly Tyr Arg Asp Leu Arg Ala Lys Leu Val Met Met Gly Met Phe Lys 
        115                 120                 125             


Ser Ser Lys Met Tyr Tyr Ala Tyr Lys Cys Ser Phe Asn Met Cys Met 
    130                 135                 140                 


Trp Leu Val Ala Val Ala Met Val Tyr Tyr Ser Asp Ser Leu Ala Met 
145                 150                 155                 160 


His Ile Gly Ser Ala Leu Leu Leu Gly Leu Phe Trp Gln Gln Cys Gly 
                165                 170                 175     


Trp Leu Ala His Asp Phe Leu His His Gln Val Phe Lys Gln Arg Lys 
            180                 185                 190         


Tyr Gly Asp Leu Val Gly Ile Phe Trp Gly Asp Leu Met Gln Gly Phe 
        195                 200                 205             


Ser Met Gln Trp Trp Lys Asn Lys His Asn Gly His His Ala Val Pro 
    210                 215                 220                 


Asn Leu His Asn Ser Ser Leu Asp Ser Gln Asp Gly Asp Pro Asp Ile 
225                 230                 235                 240 


Asp Thr Met Pro Leu Leu Ala Trp Ser Leu Lys Gln Ala Gln Ser Phe 
                245                 250                 255     


Arg Glu Ile Asn Lys Gly Lys Asp Ser Thr Phe Val Lys Tyr Ala Ile 
            260                 265                 270         


Lys Phe Gln Ala Phe Thr Tyr Phe Pro Ile Leu Leu Leu Ala Arg Ile 
        275                 280                 285             


Ser Trp Leu Asn Glu Ser Phe Lys Thr Ala Phe Gly Leu Gly Ala Ala 
    290                 295                 300                 


Ser Glu Asn Ala Lys Leu Glu Leu Glu Lys Arg Gly Leu Gln Tyr Pro 
305                 310                 315                 320 


Leu Leu Glu Lys Leu Gly Ile Thr Leu His Tyr Thr Trp Met Phe Val 
                325                 330                 335     


Leu Ser Ser Gly Phe Gly Arg Trp Ser Leu Pro Tyr Ser Ile Met Tyr 
            340                 345                 350         


Phe Phe Thr Ala Thr Cys Ser Ser Gly Leu Phe Leu Ala Leu Val Phe 
        355                 360                 365             


Gly Leu Gly His Asn Gly Met Ser Val Tyr Asp Ala Thr Thr Arg Pro 
    370                 375                 380                 


Asp Phe Trp Gln Leu Gln Val Thr Thr Thr Arg Asn Ile Ile Gly Gly 
385                 390                 395                 400 


His Gly Ile Pro Gln Phe Phe Val Asp Trp Phe Cys Gly Gly Leu Gln 
                405                 410                 415     


Tyr Gln Val Asp His His Leu Phe Pro Met Met Pro Arg Asn Asn Ile 
            420                 425                 430         


Ala Lys Cys His Lys Leu Val Glu Ser Phe Cys Lys Glu Trp Gly Val 
        435                 440                 445             


Lys Tyr His Glu Ala Asp Met Trp Asp Gly Thr Val Glu Val Leu Gln 
    450                 455                 460                 


His Leu Ser Lys Val Ser Asp Asp Phe Leu Val Glu Met Val Lys Asp 
465                 470                 475                 480 


Phe Pro Ala Met 
                

<210>  39

<211>  1449

<212>  DNA

<213>  Thalassiosira pseudonana



<220>

<221>  CDS

<222>  (1)..(1449)

<223>  Delta-5-Desaturase



<400>  39
atg cca ccc aac gcc gag gtc aaa aac ctc cgt tca cgt tcc atc cca       48
Met Pro Pro Asn Ala Glu Val Lys Asn Leu Arg Ser Arg Ser Ile Pro         
1               5                   10                  15              

acg aag aag tcc agt tca tcg tca tcc acc gcg aac gac gat ccg gct       96
Thr Lys Lys Ser Ser Ser Ser Ser Ser Thr Ala Asn Asp Asp Pro Ala         
            20                  25                  30                  

acc caa tcc acc tca cct gtg aac cga acc ctc aag tct ttg aat gga      144
Thr Gln Ser Thr Ser Pro Val Asn Arg Thr Leu Lys Ser Leu Asn Gly         
        35                  40                  45                      

aac gaa ata gct att gac ggt gtc atc tat gat att gat ggc ttt gtc      192
Asn Glu Ile Ala Ile Asp Gly Val Ile Tyr Asp Ile Asp Gly Phe Val         
    50                  55                  60                          

cat cct gga gga gag gtt att agc ttc ttt gga ggc aac gat gtg act      240
His Pro Gly Gly Glu Val Ile Ser Phe Phe Gly Gly Asn Asp Val Thr         
65                  70                  75                  80          

gta cag tac aaa atg att cat ccg tat cat aat agt aag cat ctc gag      288
Val Gln Tyr Lys Met Ile His Pro Tyr His Asn Ser Lys His Leu Glu         
                85                  90                  95              

aag atg aga gcc gtt gga aag att gca gac tac tcc aca gag tac aag      336
Lys Met Arg Ala Val Gly Lys Ile Ala Asp Tyr Ser Thr Glu Tyr Lys         
            100                 105                 110                 

ttc gac aca ccc ttt gaa cga gag atc aaa tcc gaa gtg ttc aaa atc      384
Phe Asp Thr Pro Phe Glu Arg Glu Ile Lys Ser Glu Val Phe Lys Ile         
        115                 120                 125                     

gtc cgt cga gga cgt gaa ttc ggt aca aca gga tat ttc ctc cgt gcc      432
Val Arg Arg Gly Arg Glu Phe Gly Thr Thr Gly Tyr Phe Leu Arg Ala         
    130                 135                 140                         

ttc ttc tac att gct ctc ttc ttc acc atg caa tac acc ttc gcc aca      480
Phe Phe Tyr Ile Ala Leu Phe Phe Thr Met Gln Tyr Thr Phe Ala Thr         
145                 150                 155                 160         

tgc act acc ttc acc acc tac gat cat tgg tat caa agt ggt gta ttc      528
Cys Thr Thr Phe Thr Thr Tyr Asp His Trp Tyr Gln Ser Gly Val Phe         
                165                 170                 175             

atc gcc att gtg ttt ggt atc tca caa gct ttc att ggg ttg aat gta      576
Ile Ala Ile Val Phe Gly Ile Ser Gln Ala Phe Ile Gly Leu Asn Val         
            180                 185                 190                 

caa cat gat gcc aat cac gga gct gct agc aaa cga cct tgg gtg aat      624
Gln His Asp Ala Asn His Gly Ala Ala Ser Lys Arg Pro Trp Val Asn         
        195                 200                 205                     

gat ctc ctt gga tct gga gct gat ctc atc ggt gga tgc aaa tgg aac      672
Asp Leu Leu Gly Ser Gly Ala Asp Leu Ile Gly Gly Cys Lys Trp Asn         
    210                 215                 220                         

tgg ttg gct cag cat tgg act cat cat gcg tat acc aat cac gct gat      720
Trp Leu Ala Gln His Trp Thr His His Ala Tyr Thr Asn His Ala Asp         
225                 230                 235                 240         

aaa gat cct gat agc ttt agt tcc gag ccg gtc ttc aac ttt aac gat      768
Lys Asp Pro Asp Ser Phe Ser Ser Glu Pro Val Phe Asn Phe Asn Asp         
                245                 250                 255             

tat ccc att ggt cac ccc aaa aga aag tgg tgg cat agg ttc caa ggg      816
Tyr Pro Ile Gly His Pro Lys Arg Lys Trp Trp His Arg Phe Gln Gly         
            260                 265                 270                 

ctc tac ttc cta atc atg ctg agt ttc tat tgg gta tcg atg gta ttc      864
Leu Tyr Phe Leu Ile Met Leu Ser Phe Tyr Trp Val Ser Met Val Phe         
        275                 280                 285                     

aac cca caa gtt atc gac ctc cgt cat gct gga gct gcc tac gtt gga      912
Asn Pro Gln Val Ile Asp Leu Arg His Ala Gly Ala Ala Tyr Val Gly         
    290                 295                 300                         

ttt cag atg gag aac gac ttt atc gtc aaa cgg aga aag tat gca atg      960
Phe Gln Met Glu Asn Asp Phe Ile Val Lys Arg Arg Lys Tyr Ala Met         
305                 310                 315                 320         

gca ctt cgt gca atg tac ttc tat ttc aac atc tat tgt ccg att gtc     1008
Ala Leu Arg Ala Met Tyr Phe Tyr Phe Asn Ile Tyr Cys Pro Ile Val         
                325                 330                 335             

aac aat gga ttg act tgg tcg aca gtt gga atc atc ctc tta atg gga     1056
Asn Asn Gly Leu Thr Trp Ser Thr Val Gly Ile Ile Leu Leu Met Gly         
            340                 345                 350                 

gtt agc gaa agc ttc atg ctc tcc ggt cta ttc gta ctc tca cac aac     1104
Val Ser Glu Ser Phe Met Leu Ser Gly Leu Phe Val Leu Ser His Asn         
        355                 360                 365                     

ttt gaa aat tcc gaa cgt gat cct acc tct gag tat cgc aag act ggt     1152
Phe Glu Asn Ser Glu Arg Asp Pro Thr Ser Glu Tyr Arg Lys Thr Gly         
    370                 375                 380                         

gag caa gta tgt tgg ttc aag tct caa gtg gag act tct tct acc tac     1200
Glu Gln Val Cys Trp Phe Lys Ser Gln Val Glu Thr Ser Ser Thr Tyr         
385                 390                 395                 400         

gga ggt atc gtt gct ggg tgt ctc act ggt gga ctc aac ttt caa gtg     1248
Gly Gly Ile Val Ala Gly Cys Leu Thr Gly Gly Leu Asn Phe Gln Val         
                405                 410                 415             

gag cat cat ttg ttc ccg agg atg agc agt gct tgg tat cct ttc atc     1296
Glu His His Leu Phe Pro Arg Met Ser Ser Ala Trp Tyr Pro Phe Ile         
            420                 425                 430                 

gcg ccg aag gtt aga gag att tgt aag aag cat gga gtt aga tac gct     1344
Ala Pro Lys Val Arg Glu Ile Cys Lys Lys His Gly Val Arg Tyr Ala         
        435                 440                 445                     

tac tat ccg tac atc tgg cag aac ttg cat tct acc gtg agt tac atg     1392
Tyr Tyr Pro Tyr Ile Trp Gln Asn Leu His Ser Thr Val Ser Tyr Met         
    450                 455                 460                         

cat ggg acg gga acg gga gct aga tgg gag ctt cag ccg ttg tct gga     1440
His Gly Thr Gly Thr Gly Ala Arg Trp Glu Leu Gln Pro Leu Ser Gly         
465                 470                 475                 480         

agg gcg tag                                                         1449
Arg Ala                                                                 
                                                                        


<210>  40

<211>  482

<212>  PRT

<213>  Thalassiosira pseudonana



<400>  40

Met Pro Pro Asn Ala Glu Val Lys Asn Leu Arg Ser Arg Ser Ile Pro 
1               5                   10                  15      


Thr Lys Lys Ser Ser Ser Ser Ser Ser Thr Ala Asn Asp Asp Pro Ala 
            20                  25                  30          


Thr Gln Ser Thr Ser Pro Val Asn Arg Thr Leu Lys Ser Leu Asn Gly 
        35                  40                  45              


Asn Glu Ile Ala Ile Asp Gly Val Ile Tyr Asp Ile Asp Gly Phe Val 
    50                  55                  60                  


His Pro Gly Gly Glu Val Ile Ser Phe Phe Gly Gly Asn Asp Val Thr 
65                  70                  75                  80  


Val Gln Tyr Lys Met Ile His Pro Tyr His Asn Ser Lys His Leu Glu 
                85                  90                  95      


Lys Met Arg Ala Val Gly Lys Ile Ala Asp Tyr Ser Thr Glu Tyr Lys 
            100                 105                 110         


Phe Asp Thr Pro Phe Glu Arg Glu Ile Lys Ser Glu Val Phe Lys Ile 
        115                 120                 125             


Val Arg Arg Gly Arg Glu Phe Gly Thr Thr Gly Tyr Phe Leu Arg Ala 
    130                 135                 140                 


Phe Phe Tyr Ile Ala Leu Phe Phe Thr Met Gln Tyr Thr Phe Ala Thr 
145                 150                 155                 160 


Cys Thr Thr Phe Thr Thr Tyr Asp His Trp Tyr Gln Ser Gly Val Phe 
                165                 170                 175     


Ile Ala Ile Val Phe Gly Ile Ser Gln Ala Phe Ile Gly Leu Asn Val 
            180                 185                 190         


Gln His Asp Ala Asn His Gly Ala Ala Ser Lys Arg Pro Trp Val Asn 
        195                 200                 205             


Asp Leu Leu Gly Ser Gly Ala Asp Leu Ile Gly Gly Cys Lys Trp Asn 
    210                 215                 220                 


Trp Leu Ala Gln His Trp Thr His His Ala Tyr Thr Asn His Ala Asp 
225                 230                 235                 240 


Lys Asp Pro Asp Ser Phe Ser Ser Glu Pro Val Phe Asn Phe Asn Asp 
                245                 250                 255     


Tyr Pro Ile Gly His Pro Lys Arg Lys Trp Trp His Arg Phe Gln Gly 
            260                 265                 270         


Leu Tyr Phe Leu Ile Met Leu Ser Phe Tyr Trp Val Ser Met Val Phe 
        275                 280                 285             


Asn Pro Gln Val Ile Asp Leu Arg His Ala Gly Ala Ala Tyr Val Gly 
    290                 295                 300                 


Phe Gln Met Glu Asn Asp Phe Ile Val Lys Arg Arg Lys Tyr Ala Met 
305                 310                 315                 320 


Ala Leu Arg Ala Met Tyr Phe Tyr Phe Asn Ile Tyr Cys Pro Ile Val 
                325                 330                 335     


Asn Asn Gly Leu Thr Trp Ser Thr Val Gly Ile Ile Leu Leu Met Gly 
            340                 345                 350         


Val Ser Glu Ser Phe Met Leu Ser Gly Leu Phe Val Leu Ser His Asn 
        355                 360                 365             


Phe Glu Asn Ser Glu Arg Asp Pro Thr Ser Glu Tyr Arg Lys Thr Gly 
    370                 375                 380                 


Glu Gln Val Cys Trp Phe Lys Ser Gln Val Glu Thr Ser Ser Thr Tyr 
385                 390                 395                 400 


Gly Gly Ile Val Ala Gly Cys Leu Thr Gly Gly Leu Asn Phe Gln Val 
                405                 410                 415     


Glu His His Leu Phe Pro Arg Met Ser Ser Ala Trp Tyr Pro Phe Ile 
            420                 425                 430         


Ala Pro Lys Val Arg Glu Ile Cys Lys Lys His Gly Val Arg Tyr Ala 
        435                 440                 445             


Tyr Tyr Pro Tyr Ile Trp Gln Asn Leu His Ser Thr Val Ser Tyr Met 
    450                 455                 460                 


His Gly Thr Gly Thr Gly Ala Arg Trp Glu Leu Gln Pro Leu Ser Gly 
465                 470                 475                 480 


Arg Ala 
        

<210>  41

<211>  1512

<212>  DNA

<213>  Thalassiosira pseudonana



<220>

<221>  CDS

<222>  (1)..(1512)

<223>  Delta-4-Desaturase



<400>  41
atg tgc aac ggc aac ctc cca gca tcc acc gca cag ctc aag tcc acc       48
Met Cys Asn Gly Asn Leu Pro Ala Ser Thr Ala Gln Leu Lys Ser Thr         
1               5                   10                  15              

tcg aag ccc cag cag caa cat gag cat cgc acc atc tcc aag tcc gag       96
Ser Lys Pro Gln Gln Gln His Glu His Arg Thr Ile Ser Lys Ser Glu         
            20                  25                  30                  

ctc gcc caa cac aac acg ccc aaa tca gca tgg tgt gcc gtc cac tcc      144
Leu Ala Gln His Asn Thr Pro Lys Ser Ala Trp Cys Ala Val His Ser         
        35                  40                  45                      

act ccc gcc acc gac cca tcc cac tcc aac aac aaa caa cac gca cac      192
Thr Pro Ala Thr Asp Pro Ser His Ser Asn Asn Lys Gln His Ala His         
    50                  55                  60                          

cta gtc ctc gac att acc gac ttt gcg tcc cgc cat cca ggg gga gac      240
Leu Val Leu Asp Ile Thr Asp Phe Ala Ser Arg His Pro Gly Gly Asp         
65                  70                  75                  80          

ctc atc ctc ctc gct tcc ggc aaa gac gcc tcg gtg ctg ttt gaa aca      288
Leu Ile Leu Leu Ala Ser Gly Lys Asp Ala Ser Val Leu Phe Glu Thr         
                85                  90                  95              

tac cat cca cgt gga gtt ccg acg tct ctc att caa aag ctg cag att      336
Tyr His Pro Arg Gly Val Pro Thr Ser Leu Ile Gln Lys Leu Gln Ile         
            100                 105                 110                 

gga gtg atg gag gag gag gcg ttt cgg gat tcg ttt tac agt tgg act      384
Gly Val Met Glu Glu Glu Ala Phe Arg Asp Ser Phe Tyr Ser Trp Thr         
        115                 120                 125                     

gat tct gac ttt tat act gtg ttg aag agg agg gtt gtg gag cgg ttg      432
Asp Ser Asp Phe Tyr Thr Val Leu Lys Arg Arg Val Val Glu Arg Leu         
    130                 135                 140                         

gag gag agg ggg ttg gac agg agg gga tcg aaa gag att tgg atc aag      480
Glu Glu Arg Gly Leu Asp Arg Arg Gly Ser Lys Glu Ile Trp Ile Lys         
145                 150                 155                 160         

gct ttg ttc ttg ttg gtt gga ttt tgg tac tgt ttg tac aag atg tat      528
Ala Leu Phe Leu Leu Val Gly Phe Trp Tyr Cys Leu Tyr Lys Met Tyr         
                165                 170                 175             

act acg tcg gat atc gat cag tac ggt att gcc att gcc tat tct att      576
Thr Thr Ser Asp Ile Asp Gln Tyr Gly Ile Ala Ile Ala Tyr Ser Ile         
            180                 185                 190                 

gga atg gga acc ttt gcg gca ttc atc ggc acg tgt att caa cac gat      624
Gly Met Gly Thr Phe Ala Ala Phe Ile Gly Thr Cys Ile Gln His Asp         
        195                 200                 205                     

gga aat cac ggt gca ttc gct cag aac aag tta ctc aac aag ttg gct      672
Gly Asn His Gly Ala Phe Ala Gln Asn Lys Leu Leu Asn Lys Leu Ala         
    210                 215                 220                         

ggg tgg acg ttg gat atg att ggt gcg agt gcg ttt acg tgg gag ctt      720
Gly Trp Thr Leu Asp Met Ile Gly Ala Ser Ala Phe Thr Trp Glu Leu         
225                 230                 235                 240         

cag cac atg ctg ggg cat cat cca tat acg aat gtg ttg gat ggg gtg      768
Gln His Met Leu Gly His His Pro Tyr Thr Asn Val Leu Asp Gly Val         
                245                 250                 255             

gag gag gag agg aag gag agg ggg gag gat gtt gct ttg gaa gaa aag      816
Glu Glu Glu Arg Lys Glu Arg Gly Glu Asp Val Ala Leu Glu Glu Lys         
            260                 265                 270                 

gat cag gat ttt gaa gtt gcc aca tcc gga cga tta tat cat att gat      864
Asp Gln Asp Phe Glu Val Ala Thr Ser Gly Arg Leu Tyr His Ile Asp         
        275                 280                 285                     

gcc aat gta cgt tat ggt tcg gta tgg aat gtc atg agg ttt tgg gct      912
Ala Asn Val Arg Tyr Gly Ser Val Trp Asn Val Met Arg Phe Trp Ala         
    290                 295                 300                         

atg aag gtc att acg atg gga tat atg atg gga tta cca atc tac ttt      960
Met Lys Val Ile Thr Met Gly Tyr Met Met Gly Leu Pro Ile Tyr Phe         
305                 310                 315                 320         

cat gga gta ctg agg gga gtt gga ttg ttt gtt att ggg cat ttg gcg     1008
His Gly Val Leu Arg Gly Val Gly Leu Phe Val Ile Gly His Leu Ala         
                325                 330                 335             

tgt gga gag ttg ttg gcg acg atg ttt att gtg aat cac gtc att gag     1056
Cys Gly Glu Leu Leu Ala Thr Met Phe Ile Val Asn His Val Ile Glu         
            340                 345                 350                 

ggt gtg agt tat gga acg aag gat ttg gtt ggt ggt gcg agt cat gta     1104
Gly Val Ser Tyr Gly Thr Lys Asp Leu Val Gly Gly Ala Ser His Val         
        355                 360                 365                     

gat gag aag aag att gtc aag cca acg act gta ttg gga gat aca cca     1152
Asp Glu Lys Lys Ile Val Lys Pro Thr Thr Val Leu Gly Asp Thr Pro         
    370                 375                 380                         

atg gta aag act cgc gag gag gca ttg aaa agc aac agc aat aac aac     1200
Met Val Lys Thr Arg Glu Glu Ala Leu Lys Ser Asn Ser Asn Asn Asn         
385                 390                 395                 400         

aag aag aag gga gag aag aac tcg gta cca tcc gtt cca ttc aac gac     1248
Lys Lys Lys Gly Glu Lys Asn Ser Val Pro Ser Val Pro Phe Asn Asp         
                405                 410                 415             

tgg gca gca gtc caa tgc cag acc tcc gtg aat tgg tct cca ggc tca     1296
Trp Ala Ala Val Gln Cys Gln Thr Ser Val Asn Trp Ser Pro Gly Ser         
            420                 425                 430                 

tgg ttc tgg aat cac ttt tct ggg gga ctc tct cat cag att gag cat     1344
Trp Phe Trp Asn His Phe Ser Gly Gly Leu Ser His Gln Ile Glu His         
        435                 440                 445                     

cac ttg ttc ccc agc att tgt cat aca aac tac tgt cat atc cag gat     1392
His Leu Phe Pro Ser Ile Cys His Thr Asn Tyr Cys His Ile Gln Asp         
    450                 455                 460                         

gtt gtg gag agt acg tgt gct gag tac gga gtt ccg tat cag agt gag     1440
Val Val Glu Ser Thr Cys Ala Glu Tyr Gly Val Pro Tyr Gln Ser Glu         
465                 470                 475                 480         

agt aat ttg ttt gtt gct tat gga aag atg att agt cat ttg aag ttt     1488
Ser Asn Leu Phe Val Ala Tyr Gly Lys Met Ile Ser His Leu Lys Phe         
                485                 490                 495             

ttg ggt aaa gcc aag tgt gag tag                                     1512
Leu Gly Lys Ala Lys Cys Glu                                             
            500                                                         


<210>  42

<211>  503

<212>  PRT

<213>  Thalassiosira pseudonana



<400>  42

Met Cys Asn Gly Asn Leu Pro Ala Ser Thr Ala Gln Leu Lys Ser Thr 
1               5                   10                  15      


Ser Lys Pro Gln Gln Gln His Glu His Arg Thr Ile Ser Lys Ser Glu 
            20                  25                  30          


Leu Ala Gln His Asn Thr Pro Lys Ser Ala Trp Cys Ala Val His Ser 
        35                  40                  45              


Thr Pro Ala Thr Asp Pro Ser His Ser Asn Asn Lys Gln His Ala His 
    50                  55                  60                  


Leu Val Leu Asp Ile Thr Asp Phe Ala Ser Arg His Pro Gly Gly Asp 
65                  70                  75                  80  


Leu Ile Leu Leu Ala Ser Gly Lys Asp Ala Ser Val Leu Phe Glu Thr 
                85                  90                  95      


Tyr His Pro Arg Gly Val Pro Thr Ser Leu Ile Gln Lys Leu Gln Ile 
            100                 105                 110         


Gly Val Met Glu Glu Glu Ala Phe Arg Asp Ser Phe Tyr Ser Trp Thr 
        115                 120                 125             


Asp Ser Asp Phe Tyr Thr Val Leu Lys Arg Arg Val Val Glu Arg Leu 
    130                 135                 140                 


Glu Glu Arg Gly Leu Asp Arg Arg Gly Ser Lys Glu Ile Trp Ile Lys 
145                 150                 155                 160 


Ala Leu Phe Leu Leu Val Gly Phe Trp Tyr Cys Leu Tyr Lys Met Tyr 
                165                 170                 175     


Thr Thr Ser Asp Ile Asp Gln Tyr Gly Ile Ala Ile Ala Tyr Ser Ile 
            180                 185                 190         


Gly Met Gly Thr Phe Ala Ala Phe Ile Gly Thr Cys Ile Gln His Asp 
        195                 200                 205             


Gly Asn His Gly Ala Phe Ala Gln Asn Lys Leu Leu Asn Lys Leu Ala 
    210                 215                 220                 


Gly Trp Thr Leu Asp Met Ile Gly Ala Ser Ala Phe Thr Trp Glu Leu 
225                 230                 235                 240 


Gln His Met Leu Gly His His Pro Tyr Thr Asn Val Leu Asp Gly Val 
                245                 250                 255     


Glu Glu Glu Arg Lys Glu Arg Gly Glu Asp Val Ala Leu Glu Glu Lys 
            260                 265                 270         


Asp Gln Asp Phe Glu Val Ala Thr Ser Gly Arg Leu Tyr His Ile Asp 
        275                 280                 285             


Ala Asn Val Arg Tyr Gly Ser Val Trp Asn Val Met Arg Phe Trp Ala 
    290                 295                 300                 


Met Lys Val Ile Thr Met Gly Tyr Met Met Gly Leu Pro Ile Tyr Phe 
305                 310                 315                 320 


His Gly Val Leu Arg Gly Val Gly Leu Phe Val Ile Gly His Leu Ala 
                325                 330                 335     


Cys Gly Glu Leu Leu Ala Thr Met Phe Ile Val Asn His Val Ile Glu 
            340                 345                 350         


Gly Val Ser Tyr Gly Thr Lys Asp Leu Val Gly Gly Ala Ser His Val 
        355                 360                 365             


Asp Glu Lys Lys Ile Val Lys Pro Thr Thr Val Leu Gly Asp Thr Pro 
    370                 375                 380                 


Met Val Lys Thr Arg Glu Glu Ala Leu Lys Ser Asn Ser Asn Asn Asn 
385                 390                 395                 400 


Lys Lys Lys Gly Glu Lys Asn Ser Val Pro Ser Val Pro Phe Asn Asp 
                405                 410                 415     


Trp Ala Ala Val Gln Cys Gln Thr Ser Val Asn Trp Ser Pro Gly Ser 
            420                 425                 430         


Trp Phe Trp Asn His Phe Ser Gly Gly Leu Ser His Gln Ile Glu His 
        435                 440                 445             


His Leu Phe Pro Ser Ile Cys His Thr Asn Tyr Cys His Ile Gln Asp 
    450                 455                 460                 


Val Val Glu Ser Thr Cys Ala Glu Tyr Gly Val Pro Tyr Gln Ser Glu 
465                 470                 475                 480 


Ser Asn Leu Phe Val Ala Tyr Gly Lys Met Ile Ser His Leu Lys Phe 
                485                 490                 495     


Leu Gly Lys Ala Lys Cys Glu 
            500             


<210>  43

<211>  1305

<212>  DNA

<213>  Thalassiosira pseudonana



<220>

<221>  CDS

<222>  (1)..(1305)

<223>  Delta-12-Desaturase



<400>  43
atg gga aag gga gga aga tca gta acc cgc gct caa aca gca gaa aag       48
Met Gly Lys Gly Gly Arg Ser Val Thr Arg Ala Gln Thr Ala Glu Lys         
1               5                   10                  15              

tca gca cac acc atc caa acc ttc acc gac ggc cga tgg gtc tcc ccc       96
Ser Ala His Thr Ile Gln Thr Phe Thr Asp Gly Arg Trp Val Ser Pro         
            20                  25                  30                  

tac aac ccc ctc gca aaa gat gca cct gaa ctc ccc tcc aag ggt gaa      144
Tyr Asn Pro Leu Ala Lys Asp Ala Pro Glu Leu Pro Ser Lys Gly Glu         
        35                  40                  45                      

atc aag gcg gtc atc ccc aaa gag tgc ttc gaa cga agc tac ctc cac      192
Ile Lys Ala Val Ile Pro Lys Glu Cys Phe Glu Arg Ser Tyr Leu His         
    50                  55                  60                          

tcc atg tac ttc gtc ctc cgt gac acc gtc atg gcc gtg gcc tgc gcc      240
Ser Met Tyr Phe Val Leu Arg Asp Thr Val Met Ala Val Ala Cys Ala         
65                  70                  75                  80          

tac atc gcc cac tca acg ctc tcc acc gat att ccc tcc gag tta ctg      288
Tyr Ile Ala His Ser Thr Leu Ser Thr Asp Ile Pro Ser Glu Leu Leu         
                85                  90                  95              

agc gtg gac gca ctc aaa tgg ttc ctc gga tgg aac acc tac gcc ttt      336
Ser Val Asp Ala Leu Lys Trp Phe Leu Gly Trp Asn Thr Tyr Ala Phe         
            100                 105                 110                 

tgg atg ggg tgc att ctc acc gga cac tgg gtc cta gcc cat gaa tgt      384
Trp Met Gly Cys Ile Leu Thr Gly His Trp Val Leu Ala His Glu Cys         
        115                 120                 125                     

gga cat ggt gca ttc tct ccc tct cag acg ttt aat gac ttt tgg ggg      432
Gly His Gly Ala Phe Ser Pro Ser Gln Thr Phe Asn Asp Phe Trp Gly         
    130                 135                 140                         

ttc att atg cat cag gcg gtg ttg gtt ccg tat ttc gcc tgg cag tac      480
Phe Ile Met His Gln Ala Val Leu Val Pro Tyr Phe Ala Trp Gln Tyr         
145                 150                 155                 160         

tct cat gcg aag cat cat cga cgt acc aac aac att atg gat ggg gag      528
Ser His Ala Lys His His Arg Arg Thr Asn Asn Ile Met Asp Gly Glu         
                165                 170                 175             

agc cat gtg ccc aat atc gcc aag gaa atg gga ttg aac gag aag aat      576
Ser His Val Pro Asn Ile Ala Lys Glu Met Gly Leu Asn Glu Lys Asn         
            180                 185                 190                 

gag cgc agt gga gga tat gcc gcc att cat gag gct att gga gat gga      624
Glu Arg Ser Gly Gly Tyr Ala Ala Ile His Glu Ala Ile Gly Asp Gly         
        195                 200                 205                     

ccc ttt gcg atg ttt caa atc ttt gct cac ttg gtg atc ggg tgg cct      672
Pro Phe Ala Met Phe Gln Ile Phe Ala His Leu Val Ile Gly Trp Pro         
    210                 215                 220                         

att tac ttg atg gga ttt gct tcc act gga cgt ctc ggt cag gat ggg      720
Ile Tyr Leu Met Gly Phe Ala Ser Thr Gly Arg Leu Gly Gln Asp Gly         
225                 230                 235                 240         

aag gaa ctt cag gct gga gag atc atc gac cat tac cgt cct tgg agt      768
Lys Glu Leu Gln Ala Gly Glu Ile Ile Asp His Tyr Arg Pro Trp Ser         
                245                 250                 255             

aag atg ttc ccc acc aag ttg cga ttc aaa att gct ctt tcg aca ctt      816
Lys Met Phe Pro Thr Lys Leu Arg Phe Lys Ile Ala Leu Ser Thr Leu         
            260                 265                 270                 

gga gtg att gcc gcc tgg gtt ggg ttg tac ttt gct gca caa gag tat      864
Gly Val Ile Ala Ala Trp Val Gly Leu Tyr Phe Ala Ala Gln Glu Tyr         
        275                 280                 285                     

gga gtc ttg ccc gtg gtt ctt tgg tac att ggc cca ctc atg tgg aat      912
Gly Val Leu Pro Val Val Leu Trp Tyr Ile Gly Pro Leu Met Trp Asn         
    290                 295                 300                         

cag gcg tgg ctt gtg ctc tac act tgg ctt cag cac aat gat ccc tcc      960
Gln Ala Trp Leu Val Leu Tyr Thr Trp Leu Gln His Asn Asp Pro Ser         
305                 310                 315                 320         

gtg cct caa tat gga agt gac gaa tgg aca tgg gtc aag gga gct ttg     1008
Val Pro Gln Tyr Gly Ser Asp Glu Trp Thr Trp Val Lys Gly Ala Leu         
                325                 330                 335             

tcg acg att gat cgc ccg tat ggt atc ttt gac ttc ttc cat cac aag     1056
Ser Thr Ile Asp Arg Pro Tyr Gly Ile Phe Asp Phe Phe His His Lys         
            340                 345                 350                 

att gga agc act cac gta gct cat cat ttg ttc cac gag atg cca ttt     1104
Ile Gly Ser Thr His Val Ala His His Leu Phe His Glu Met Pro Phe         
        355                 360                 365                     

tac aag gcg gat gtg gct act gcg tcg atc aag ggt ttc ttg gag ccg     1152
Tyr Lys Ala Asp Val Ala Thr Ala Ser Ile Lys Gly Phe Leu Glu Pro         
    370                 375                 380                         

aag gga ctt tac aac tat gat cca acg cct tgg tat gtg gcc atg tgg     1200
Lys Gly Leu Tyr Asn Tyr Asp Pro Thr Pro Trp Tyr Val Ala Met Trp         
385                 390                 395                 400         

agg gtg gcc aag act tgt cat tat att gag gat gtg gat gga gtt cag     1248
Arg Val Ala Lys Thr Cys His Tyr Ile Glu Asp Val Asp Gly Val Gln         
                405                 410                 415             

tat tat aag agt ttg gag gat gtg cct ttg aag aag gat gcc aag aag     1296
Tyr Tyr Lys Ser Leu Glu Asp Val Pro Leu Lys Lys Asp Ala Lys Lys         
            420                 425                 430                 

tct gat tag                                                         1305
Ser Asp                                                                 
                                                                        


<210>  44

<211>  434

<212>  PRT

<213>  Thalassiosira pseudonana



<400>  44

Met Gly Lys Gly Gly Arg Ser Val Thr Arg Ala Gln Thr Ala Glu Lys 
1               5                   10                  15      


Ser Ala His Thr Ile Gln Thr Phe Thr Asp Gly Arg Trp Val Ser Pro 
            20                  25                  30          


Tyr Asn Pro Leu Ala Lys Asp Ala Pro Glu Leu Pro Ser Lys Gly Glu 
        35                  40                  45              


Ile Lys Ala Val Ile Pro Lys Glu Cys Phe Glu Arg Ser Tyr Leu His 
    50                  55                  60                  


Ser Met Tyr Phe Val Leu Arg Asp Thr Val Met Ala Val Ala Cys Ala 
65                  70                  75                  80  


Tyr Ile Ala His Ser Thr Leu Ser Thr Asp Ile Pro Ser Glu Leu Leu 
                85                  90                  95      


Ser Val Asp Ala Leu Lys Trp Phe Leu Gly Trp Asn Thr Tyr Ala Phe 
            100                 105                 110         


Trp Met Gly Cys Ile Leu Thr Gly His Trp Val Leu Ala His Glu Cys 
        115                 120                 125             


Gly His Gly Ala Phe Ser Pro Ser Gln Thr Phe Asn Asp Phe Trp Gly 
    130                 135                 140                 


Phe Ile Met His Gln Ala Val Leu Val Pro Tyr Phe Ala Trp Gln Tyr 
145                 150                 155                 160 


Ser His Ala Lys His His Arg Arg Thr Asn Asn Ile Met Asp Gly Glu 
                165                 170                 175     


Ser His Val Pro Asn Ile Ala Lys Glu Met Gly Leu Asn Glu Lys Asn 
            180                 185                 190         


Glu Arg Ser Gly Gly Tyr Ala Ala Ile His Glu Ala Ile Gly Asp Gly 
        195                 200                 205             


Pro Phe Ala Met Phe Gln Ile Phe Ala His Leu Val Ile Gly Trp Pro 
    210                 215                 220                 


Ile Tyr Leu Met Gly Phe Ala Ser Thr Gly Arg Leu Gly Gln Asp Gly 
225                 230                 235                 240 


Lys Glu Leu Gln Ala Gly Glu Ile Ile Asp His Tyr Arg Pro Trp Ser 
                245                 250                 255     


Lys Met Phe Pro Thr Lys Leu Arg Phe Lys Ile Ala Leu Ser Thr Leu 
            260                 265                 270         


Gly Val Ile Ala Ala Trp Val Gly Leu Tyr Phe Ala Ala Gln Glu Tyr 
        275                 280                 285             


Gly Val Leu Pro Val Val Leu Trp Tyr Ile Gly Pro Leu Met Trp Asn 
    290                 295                 300                 


Gln Ala Trp Leu Val Leu Tyr Thr Trp Leu Gln His Asn Asp Pro Ser 
305                 310                 315                 320 


Val Pro Gln Tyr Gly Ser Asp Glu Trp Thr Trp Val Lys Gly Ala Leu 
                325                 330                 335     


Ser Thr Ile Asp Arg Pro Tyr Gly Ile Phe Asp Phe Phe His His Lys 
            340                 345                 350         


Ile Gly Ser Thr His Val Ala His His Leu Phe His Glu Met Pro Phe 
        355                 360                 365             


Tyr Lys Ala Asp Val Ala Thr Ala Ser Ile Lys Gly Phe Leu Glu Pro 
    370                 375                 380                 


Lys Gly Leu Tyr Asn Tyr Asp Pro Thr Pro Trp Tyr Val Ala Met Trp 
385                 390                 395                 400 


Arg Val Ala Lys Thr Cys His Tyr Ile Glu Asp Val Asp Gly Val Gln 
                405                 410                 415     


Tyr Tyr Lys Ser Leu Glu Asp Val Pro Leu Lys Lys Asp Ala Lys Lys 
            420                 425                 430         


Ser Asp 
        
