
                                SEQUENCE LISTING

<110> El Kasmi, Karim
      Jordan, Brad
      Kriwacki, Richard
      Mann, Beth
      Orihuela, Carlos
      Tuomanen, Elaine


<120> Synthetic Streptococcus Pneumoniae
  Vaccine

<130> 044158/332780

<150> 60/847,446          
<151> 2006-09-27  

<160> 74
<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 693
<212> PRT
<213> Streptococcus pneumoniae

<400> 1
Met Phe Ala Ser Lys Ser Glu Arg Lys Val His Tyr Ser Ile Arg Lys
 1               5                  10                  15      
Phe Ser Val Gly Val Ala Ser Val Val Val Ala Ser Leu Val Met Gly
            20                  25                  30          
Ser Val Val His Ala Thr Glu Asn Glu Gly Ala Thr Gln Val Pro Thr
        35                  40                  45              
Ser Ser Asn Arg Ala Asn Glu Ser Gln Ala Glu Gln Gly Glu Gln Pro
    50                  55                  60                  
Lys Lys Leu Asp Ser Glu Arg Asp Lys Ala Arg Lys Glu Val Glu Glu
65                  70                  75                  80  
Tyr Val Lys Lys Ile Val Gly Glu Ser Tyr Ala Lys Ser Thr Lys Lys
                85                  90                  95      
Arg His Thr Ile Thr Val Ala Leu Val Asn Glu Leu Asn Asn Ile Lys
            100                 105                 110         
Asn Glu Tyr Leu Asn Lys Ile Val Glu Ser Thr Ser Glu Ser Gln Leu
        115                 120                 125             
Gln Ile Leu Met Met Glu Ser Arg Ser Lys Val Asp Glu Ala Val Ser
    130                 135                 140                 
Lys Phe Glu Lys Asp Ser Ser Ser Ser Ser Ser Ser Asp Ser Ser Thr
145                 150                 155                 160 
Lys Pro Glu Ala Ser Asp Thr Ala Lys Pro Asn Lys Pro Thr Glu Pro
                165                 170                 175     
Gly Glu Lys Val Ala Glu Ala Lys Lys Lys Val Glu Glu Ala Glu Lys
            180                 185                 190         
Lys Ala Lys Asp Gln Lys Glu Glu Asp Arg Arg Asn Tyr Pro Thr Ile
        195                 200                 205             
Thr Tyr Lys Thr Leu Glu Leu Glu Ile Ala Glu Ser Asp Val Glu Val
    210                 215                 220                 
Lys Lys Ala Glu Leu Glu Leu Val Lys Val Lys Ala Asn Glu Pro Arg
225                 230                 235                 240 
Asp Glu Gln Lys Ile Lys Gln Ala Glu Ala Glu Val Glu Ser Lys Gln
                245                 250                 255     
Ala Glu Ala Thr Arg Leu Lys Lys Ile Lys Thr Asp Arg Glu Glu Ala
            260                 265                 270         
Glu Glu Glu Ala Lys Arg Arg Ala Asp Ala Lys Glu Gln Gly Lys Pro
        275                 280                 285             
Lys Gly Arg Ala Lys Arg Gly Val Pro Gly Glu Leu Ala Thr Pro Asp
    290                 295                 300                 
Lys Lys Glu Asn Asp Ala Lys Ser Ser Asp Ser Ser Val Gly Glu Glu
305                 310                 315                 320 
Thr Leu Pro Ser Pro Ser Leu Lys Pro Glu Lys Lys Val Ala Glu Ala
                325                 330                 335     
Glu Lys Lys Val Glu Glu Ala Lys Lys Lys Ala Glu Asp Gln Lys Glu
            340                 345                 350         
Glu Asp Arg Arg Asn Tyr Pro Thr Asn Thr Tyr Lys Thr Leu Glu Leu
        355                 360                 365             
Glu Ile Ala Glu Ser Asp Val Glu Val Lys Lys Ala Glu Leu Glu Leu
    370                 375                 380                 
Val Lys Glu Glu Ala Lys Glu Pro Arg Asn Glu Glu Lys Val Lys Gln
385                 390                 395                 400 
Ala Lys Ala Glu Val Glu Ser Lys Lys Ala Glu Ala Thr Arg Leu Glu
                405                 410                 415     
Lys Ile Lys Thr Asp Arg Lys Lys Ala Glu Glu Glu Ala Lys Arg Lys
            420                 425                 430         
Ala Ala Glu Glu Asp Lys Val Lys Glu Lys Pro Ala Glu Gln Pro Gln
        435                 440                 445             
Pro Ala Pro Ala Pro Lys Ala Glu Lys Pro Ala Pro Ala Pro Lys Pro
    450                 455                 460                 
Glu Asn Pro Ala Glu Gln Pro Lys Ala Glu Lys Pro Ala Asp Gln Gln
465                 470                 475                 480 
Ala Glu Glu Asp Tyr Ala Arg Arg Ser Glu Glu Glu Tyr Asn Arg Leu
                485                 490                 495     
Thr Gln Gln Gln Pro Pro Lys Thr Glu Lys Pro Ala Gln Pro Ser Thr
            500                 505                 510         
Pro Lys Thr Gly Trp Lys Gln Glu Asn Gly Met Trp Tyr Phe Tyr Asn
        515                 520                 525             
Thr Asp Gly Ser Met Ala Thr Gly Trp Leu Gln Asn Asn Gly Ser Trp
    530                 535                 540                 
Tyr Tyr Leu Asn Ser Asn Gly Ala Met Ala Thr Gly Trp Leu Gln Asn
545                 550                 555                 560 
Asn Gly Ser Trp Tyr Tyr Leu Asn Ala Asn Gly Ser Met Ala Thr Gly
                565                 570                 575     
Trp Leu Gln Asn Asn Gly Ser Trp Tyr Tyr Leu Asn Ala Asn Gly Ser
            580                 585                 590         
Met Ala Thr Gly Trp Leu Gln Tyr Asn Gly Ser Trp Tyr Tyr Leu Asn
        595                 600                 605             
Ala Asn Gly Ser Met Ala Thr Gly Trp Leu Gln Tyr Asn Gly Ser Trp
    610                 615                 620                 
Tyr Tyr Leu Asn Ala Asn Gly Asp Met Ala Thr Gly Trp Val Lys Asp
625                 630                 635                 640 
Gly Asp Thr Trp Tyr Tyr Leu Glu Ala Ser Gly Ala Met Lys Ala Ser
                645                 650                 655     
Gln Trp Phe Lys Val Ser Asp Lys Trp Tyr Tyr Val Asn Gly Ser Gly
            660                 665                 670         
Ala Leu Ala Val Asn Thr Thr Val Asp Gly Tyr Gly Val Asn Ala Asn
        675                 680                 685             
Gly Glu Trp Val Asn
    690             


<210> 2
<211> 2082
<212> DNA
<213> Streptococcus pneumoniae

<220> 
<221> CDS            
<222> (1)...(2082)

<221> misc_feature   
<222> (0)...(0)
<223> nucleotide sequence encoding the full length CbpA
      of SEQ ID NO:1 (GenBank Accession No. AE005672)

<400> 2
atg ttt gca tca aaa agc gaa aga aaa gta cat tat tca att cgt aaa   48
Met Phe Ala Ser Lys Ser Glu Arg Lys Val His Tyr Ser Ile Arg Lys
 1               5                   10                  15
 
ttt agt gtt gga gta gct agt gta gtt gtt gcc agt ctt gtt atg gga   96
Phe Ser Val Gly Val Ala Ser Val Val Val Ala Ser Leu Val Met Gly
             20                  25                  30
 
agt gtg gtt cat gcg aca gag aac gag gga gct acc caa gta ccc act   144
Ser Val Val His Ala Thr Glu Asn Glu Gly Ala Thr Gln Val Pro Thr
         35                  40                  45
 
tct tct aat agg gca aat gaa agt cag gca gaa caa gga gaa caa cct   192
Ser Ser Asn Arg Ala Asn Glu Ser Gln Ala Glu Gln Gly Glu Gln Pro
     50                  55                  60
 
aaa aaa ctc gat tca gaa cga gat aag gca agg aaa gag gtc gag gaa   240
Lys Lys Leu Asp Ser Glu Arg Asp Lys Ala Arg Lys Glu Val Glu Glu
 65                  70                  75                  80
 
tat gta aaa aaa ata gtg ggt gag agc tat gca aaa tca act aaa aag   288
Tyr Val Lys Lys Ile Val Gly Glu Ser Tyr Ala Lys Ser Thr Lys Lys
                 85                  90                  95
 
cga cat aca att act gta gct cta gtt aac gag ttg aac aac att aag   336
Arg His Thr Ile Thr Val Ala Leu Val Asn Glu Leu Asn Asn Ile Lys
            100                 105                 110
 
aac gag tat ttg aat aaa ata gtt gaa tca acc tca gaa agc caa cta   384
Asn Glu Tyr Leu Asn Lys Ile Val Glu Ser Thr Ser Glu Ser Gln Leu
        115                 120                 125
 
cag ata ctg atg atg gag agt cga tca aaa gta gat gaa gct gtg tct   432
Gln Ile Leu Met Met Glu Ser Arg Ser Lys Val Asp Glu Ala Val Ser
    130                 135                 140
 
aag ttt gaa aag gac tca tct tct tcg tca agt tca gac tct tcc act   480
Lys Phe Glu Lys Asp Ser Ser Ser Ser Ser Ser Ser Asp Ser Ser Thr
145                 150                 155                 160
 
aaa ccg gaa gct tca gat aca gcg aag cca aac aag ccg aca gaa cca   528
Lys Pro Glu Ala Ser Asp Thr Ala Lys Pro Asn Lys Pro Thr Glu Pro
                165                 170                 175
 
gga gaa aag gta gca gaa gct aag aag aag gtt gaa gaa gct gag aaa   576
Gly Glu Lys Val Ala Glu Ala Lys Lys Lys Val Glu Glu Ala Glu Lys
            180                 185                 190
 
aaa gcc aag gat caa aaa gaa gaa gat cgt cgt aac tac cca acc att   624
Lys Ala Lys Asp Gln Lys Glu Glu Asp Arg Arg Asn Tyr Pro Thr Ile
        195                 200                 205
 
act tac aaa acg ctt gaa ctt gaa att gct gag tcc gat gtg gaa gtt   672
Thr Tyr Lys Thr Leu Glu Leu Glu Ile Ala Glu Ser Asp Val Glu Val
    210                 215                 220
 
aaa aaa gcg gag ctt gaa cta gta aaa gtg aaa gct aac gaa cct cga   720
Lys Lys Ala Glu Leu Glu Leu Val Lys Val Lys Ala Asn Glu Pro Arg
225                 230                 235                 240
 
gac gag caa aaa att aag caa gca gaa gcg gaa gtt gag agt aaa caa   768
Asp Glu Gln Lys Ile Lys Gln Ala Glu Ala Glu Val Glu Ser Lys Gln
                245                 250                 255
 
gct gag gct aca agg tta aaa aaa atc aag aca gat cgt gaa gaa gca   816
Ala Glu Ala Thr Arg Leu Lys Lys Ile Lys Thr Asp Arg Glu Glu Ala
            260                 265                 270
 
gaa gaa gaa gct aaa cga aga gca gat gct aaa gag caa ggt aaa cca   864
Glu Glu Glu Ala Lys Arg Arg Ala Asp Ala Lys Glu Gln Gly Lys Pro
        275                 280                 285
 
aag ggg cgg gca aaa cga gga gtt cct gga gag cta gca aca cct gat   912
Lys Gly Arg Ala Lys Arg Gly Val Pro Gly Glu Leu Ala Thr Pro Asp
    290                 295                 300
 
aaa aaa gaa aat gat gcg aag tct tca gat tct agc gta ggt gaa gaa   960
Lys Lys Glu Asn Asp Ala Lys Ser Ser Asp Ser Ser Val Gly Glu Glu
305                 310                 315                 320
 
act ctt cca agc cca tcc ctg aaa cca gaa aaa aag gta gca gaa gct   1008
Thr Leu Pro Ser Pro Ser Leu Lys Pro Glu Lys Lys Val Ala Glu Ala
                325                 330                 335
 
gag aag aag gtt gaa gaa gct aag aaa aaa gcc gag gat caa aaa gaa   1056
Glu Lys Lys Val Glu Glu Ala Lys Lys Lys Ala Glu Asp Gln Lys Glu
            340                 345                 350
 
gaa gat cgc cgt aac tac cca acc aat act tac aaa acg ctt gaa ctt   1104
Glu Asp Arg Arg Asn Tyr Pro Thr Asn Thr Tyr Lys Thr Leu Glu Leu
        355                 360                 365
 
gaa att gct gag tcc gat gtg gaa gtt aaa aaa gcg gag ctt gaa cta   1152
Glu Ile Ala Glu Ser Asp Val Glu Val Lys Lys Ala Glu Leu Glu Leu
    370                 375                 380
 
gta aaa gag gaa gct aag gaa cct cga aac gag gaa aaa gtt aag caa   1200
Val Lys Glu Glu Ala Lys Glu Pro Arg Asn Glu Glu Lys Val Lys Gln
385                 390                 395                 400
 
gca aaa gcg gaa gtt gag agt aaa aaa gct gag gct aca agg tta gaa   1248
Ala Lys Ala Glu Val Glu Ser Lys Lys Ala Glu Ala Thr Arg Leu Glu
                405                 410                 415
 
aaa atc aag aca gat cgt aaa aaa gca gaa gaa gaa gct aaa cga aaa   1296
Lys Ile Lys Thr Asp Arg Lys Lys Ala Glu Glu Glu Ala Lys Arg Lys
            420                 425                 430
 
gca gca gaa gaa gat aaa gtt aaa gaa aaa cca gct gaa caa cca caa   1344
Ala Ala Glu Glu Asp Lys Val Lys Glu Lys Pro Ala Glu Gln Pro Gln
        435                 440                 445
 
cca gcg ccg gct cca aaa gca gaa aaa cca gct cca gct cca aaa cca   1392
Pro Ala Pro Ala Pro Lys Ala Glu Lys Pro Ala Pro Ala Pro Lys Pro
    450                 455                 460
 
gag aat cca gct gaa caa cca aaa gca gaa aaa cca gct gat caa caa   1440
Glu Asn Pro Ala Glu Gln Pro Lys Ala Glu Lys Pro Ala Asp Gln Gln
465                 470                 475                 480
 
gct gaa gaa gac tat gct cgt aga tca gaa gaa gaa tat aat cgc ttg   1488
Ala Glu Glu Asp Tyr Ala Arg Arg Ser Glu Glu Glu Tyr Asn Arg Leu
                485                 490                 495
 
act caa cag caa ccg cca aaa act gaa aaa cca gca caa cca tct act   1536
Thr Gln Gln Gln Pro Pro Lys Thr Glu Lys Pro Ala Gln Pro Ser Thr
            500                 505                 510
 
cca aaa aca ggc tgg aaa caa gaa aac ggt atg tgg tac ttc tac aat   1584
Pro Lys Thr Gly Trp Lys Gln Glu Asn Gly Met Trp Tyr Phe Tyr Asn
        515                 520                 525
 
act gat ggt tca atg gcg aca gga tgg ctc caa aac aat ggc tca tgg   1632
Thr Asp Gly Ser Met Ala Thr Gly Trp Leu Gln Asn Asn Gly Ser Trp
    530                 535                 540
 
tac tac ctc aac agc aat ggc gct atg gcg aca gga tgg ctc caa aac   1680
Tyr Tyr Leu Asn Ser Asn Gly Ala Met Ala Thr Gly Trp Leu Gln Asn
545                 550                 555                 560
 
aat ggt tca tgg tac tat cta aac gct aat ggt tca atg gca aca gga   1728
Asn Gly Ser Trp Tyr Tyr Leu Asn Ala Asn Gly Ser Met Ala Thr Gly
                565                 570                 575
 
tgg ctc caa aac aat ggt tca tgg tac tac cta aac gct aat ggt tca   1776
Trp Leu Gln Asn Asn Gly Ser Trp Tyr Tyr Leu Asn Ala Asn Gly Ser
            580                 585                 590
 
atg gcg aca gga tgg ctc caa tac aat ggc tca tgg tac tac cta aac   1824
Met Ala Thr Gly Trp Leu Gln Tyr Asn Gly Ser Trp Tyr Tyr Leu Asn
        595                 600                 605
 
gct aat ggt tca atg gcg aca gga tgg ctc caa tac aat ggc tca tgg   1872
Ala Asn Gly Ser Met Ala Thr Gly Trp Leu Gln Tyr Asn Gly Ser Trp
    610                 615                 620
 
tac tac cta aac gct aat ggt gat atg gcg aca ggt tgg gtg aaa gat   1920
Tyr Tyr Leu Asn Ala Asn Gly Asp Met Ala Thr Gly Trp Val Lys Asp
625                 630                 635                 640
 
gga gat acc tgg tac tat ctt gaa gca tca ggt gct atg aaa gca agc   1968
Gly Asp Thr Trp Tyr Tyr Leu Glu Ala Ser Gly Ala Met Lys Ala Ser
                645                 650                 655
 
caa tgg ttc aaa gta tca gat aaa tgg tac tat gtc aat ggc tca ggt   2016
Gln Trp Phe Lys Val Ser Asp Lys Trp Tyr Tyr Val Asn Gly Ser Gly
            660                 665                 670
 
gcc ctt gca gtc aac aca act gta gat ggc tat gga gtc aat gcc aat   2064
Ala Leu Ala Val Asn Thr Thr Val Asp Gly Tyr Gly Val Asn Ala Asn
        675                 680                 685
 
ggt gaa tgg gta aac taa                                           2082
Gly Glu Trp Val Asn  *
    690
 

<210> 3
<211> 472
<212> PRT
<213> Streptococcus pneumoniae

<400> 3
Glu Asn Glu Gly Ala Thr Gln Val Pro Thr Ser Ser Asn Arg Ala Asn
 1               5                  10                  15      
Glu Ser Gln Ala Glu Gln Gly Glu Gln Pro Lys Lys Leu Asp Ser Glu
            20                  25                  30          
Arg Asp Lys Ala Arg Lys Glu Val Glu Glu Tyr Val Lys Lys Ile Val
        35                  40                  45              
Gly Glu Ser Tyr Ala Lys Ser Thr Lys Lys Arg His Thr Ile Thr Val
    50                  55                  60                  
Ala Leu Val Asn Glu Leu Asn Asn Ile Lys Asn Glu Tyr Leu Asn Lys
65                  70                  75                  80  
Ile Val Glu Ser Thr Ser Glu Ser Gln Leu Gln Ile Leu Met Met Glu
                85                  90                  95      
Ser Arg Ser Lys Val Asp Glu Ala Val Ser Lys Phe Glu Lys Asp Ser
            100                 105                 110         
Ser Ser Ser Ser Ser Ser Asp Ser Ser Thr Lys Pro Glu Ala Ser Asp
        115                 120                 125             
Thr Ala Lys Pro Asn Lys Pro Thr Glu Pro Gly Glu Lys Val Ala Glu
    130                 135                 140                 
Ala Lys Lys Lys Val Glu Glu Ala Glu Lys Lys Ala Lys Asp Gln Lys
145                 150                 155                 160 
Glu Glu Asp Arg Arg Asn Tyr Pro Thr Ile Thr Tyr Lys Thr Leu Glu
                165                 170                 175     
Leu Glu Ile Ala Glu Ser Asp Val Glu Val Lys Lys Ala Glu Leu Glu
            180                 185                 190         
Leu Val Lys Val Lys Ala Asn Glu Pro Arg Asp Glu Gln Lys Ile Lys
        195                 200                 205             
Gln Ala Glu Ala Glu Val Glu Ser Lys Gln Ala Glu Ala Thr Arg Leu
    210                 215                 220                 
Lys Lys Ile Lys Thr Asp Arg Glu Glu Ala Glu Glu Glu Ala Lys Arg
225                 230                 235                 240 
Arg Ala Asp Ala Lys Glu Gln Gly Lys Pro Lys Gly Arg Ala Lys Arg
                245                 250                 255     
Gly Val Pro Gly Glu Leu Ala Thr Pro Asp Lys Lys Glu Asn Asp Ala
            260                 265                 270         
Lys Ser Ser Asp Ser Ser Val Gly Glu Glu Thr Leu Pro Ser Pro Ser
        275                 280                 285             
Leu Lys Pro Glu Lys Lys Val Ala Glu Ala Glu Lys Lys Val Glu Glu
    290                 295                 300                 
Ala Lys Lys Lys Ala Glu Asp Gln Lys Glu Glu Asp Arg Arg Asn Tyr
305                 310                 315                 320 
Pro Thr Asn Thr Tyr Lys Thr Leu Glu Leu Glu Ile Ala Glu Ser Asp
                325                 330                 335     
Val Glu Val Lys Lys Ala Glu Leu Glu Leu Val Lys Glu Glu Ala Lys
            340                 345                 350         
Glu Pro Arg Asn Glu Glu Lys Val Lys Gln Ala Lys Ala Glu Val Glu
        355                 360                 365             
Ser Lys Lys Ala Glu Ala Thr Arg Leu Glu Lys Ile Lys Thr Asp Arg
    370                 375                 380                 
Lys Lys Ala Glu Glu Glu Ala Lys Arg Lys Ala Ala Glu Glu Asp Lys
385                 390                 395                 400 
Val Lys Glu Lys Pro Ala Glu Gln Pro Gln Pro Ala Pro Ala Pro Lys
                405                 410                 415     
Ala Glu Lys Pro Ala Pro Ala Pro Lys Pro Glu Asn Pro Ala Glu Gln
            420                 425                 430         
Pro Lys Ala Glu Lys Pro Ala Asp Gln Gln Ala Glu Glu Asp Tyr Ala
        435                 440                 445             
Arg Arg Ser Glu Glu Glu Tyr Asn Arg Leu Thr Gln Gln Gln Pro Pro
    450                 455                 460                 
Lys Thr Glu Lys Pro Ala Gln Pro
465                 470         


<210> 4
<211> 1416
<212> DNA
<213> Streptococcus pneumoniae

<220> 
<221> misc_feature   
<222> (0)...(0)
<223> Nucleotide sequence encoding the N-terminal amino
      acid sequence of CbpA set forth in SEQ ID NO:3,
      which corresponds to residues 39-510 of SEQ ID
      NO:1.

<221> CDS            
<222> (1)...(1416)

<400> 4
gag aac gag gga gct acc caa gta ccc act tct tct aat agg gca aat   48
Glu Asn Glu Gly Ala Thr Gln Val Pro Thr Ser Ser Asn Arg Ala Asn
 1               5                   10                  15
 
gaa agt cag gca gaa caa gga gaa caa cct aaa aaa ctc gat tca gaa   96
Glu Ser Gln Ala Glu Gln Gly Glu Gln Pro Lys Lys Leu Asp Ser Glu
             20                  25                  30
 
cga gat aag gca agg aaa gag gtc gag gaa tat gta aaa aaa ata gtg   144
Arg Asp Lys Ala Arg Lys Glu Val Glu Glu Tyr Val Lys Lys Ile Val
         35                  40                  45
 
ggt gag agc tat gca aaa tca act aaa aag cga cat aca att act gta   192
Gly Glu Ser Tyr Ala Lys Ser Thr Lys Lys Arg His Thr Ile Thr Val
     50                  55                  60
 
gct cta gtt aac gag ttg aac aac att aag aac gag tat ttg aat aaa   240
Ala Leu Val Asn Glu Leu Asn Asn Ile Lys Asn Glu Tyr Leu Asn Lys
 65                  70                  75                  80
 
ata gtt gaa tca acc tca gaa agc caa cta cag ata ctg atg atg gag   288
Ile Val Glu Ser Thr Ser Glu Ser Gln Leu Gln Ile Leu Met Met Glu
                 85                  90                  95
 
agt cga tca aaa gta gat gaa gct gtg tct aag ttt gaa aag gac tca   336
Ser Arg Ser Lys Val Asp Glu Ala Val Ser Lys Phe Glu Lys Asp Ser
            100                 105                 110
 
tct tct tcg tca agt tca gac tct tcc act aaa ccg gaa gct tca gat   384
Ser Ser Ser Ser Ser Ser Asp Ser Ser Thr Lys Pro Glu Ala Ser Asp
        115                 120                 125
 
aca gcg aag cca aac aag ccg aca gaa cca gga gaa aag gta gca gaa   432
Thr Ala Lys Pro Asn Lys Pro Thr Glu Pro Gly Glu Lys Val Ala Glu
    130                 135                 140
 
gct aag aag aag gtt gaa gaa gct gag aaa aaa gcc aag gat caa aaa   480
Ala Lys Lys Lys Val Glu Glu Ala Glu Lys Lys Ala Lys Asp Gln Lys
145                 150                 155                 160
 
gaa gaa gat cgt cgt aac tac cca acc att act tac aaa acg ctt gaa   528
Glu Glu Asp Arg Arg Asn Tyr Pro Thr Ile Thr Tyr Lys Thr Leu Glu
                165                 170                 175
 
ctt gaa att gct gag tcc gat gtg gaa gtt aaa aaa gcg gag ctt gaa   576
Leu Glu Ile Ala Glu Ser Asp Val Glu Val Lys Lys Ala Glu Leu Glu
            180                 185                 190
 
cta gta aaa gtg aaa gct aac gaa cct cga gac gag caa aaa att aag   624
Leu Val Lys Val Lys Ala Asn Glu Pro Arg Asp Glu Gln Lys Ile Lys
        195                 200                 205
 
caa gca gaa gcg gaa gtt gag agt aaa caa gct gag gct aca agg tta   672
Gln Ala Glu Ala Glu Val Glu Ser Lys Gln Ala Glu Ala Thr Arg Leu
    210                 215                 220
 
aaa aaa atc aag aca gat cgt gaa gaa gca gaa gaa gaa gct aaa cga   720
Lys Lys Ile Lys Thr Asp Arg Glu Glu Ala Glu Glu Glu Ala Lys Arg
225                 230                 235                 240
 
aga gca gat gct aaa gag caa ggt aaa cca aag ggg cgg gca aaa cga   768
Arg Ala Asp Ala Lys Glu Gln Gly Lys Pro Lys Gly Arg Ala Lys Arg
                245                 250                 255
 
gga gtt cct gga gag cta gca aca cct gat aaa aaa gaa aat gat gcg   816
Gly Val Pro Gly Glu Leu Ala Thr Pro Asp Lys Lys Glu Asn Asp Ala
            260                 265                 270
 
aag tct tca gat tct agc gta ggt gaa gaa act ctt cca agc cca tcc   864
Lys Ser Ser Asp Ser Ser Val Gly Glu Glu Thr Leu Pro Ser Pro Ser
        275                 280                 285
 
ctg aaa cca gaa aaa aag gta gca gaa gct gag aag aag gtt gaa gaa   912
Leu Lys Pro Glu Lys Lys Val Ala Glu Ala Glu Lys Lys Val Glu Glu
    290                 295                 300
 
gct aag aaa aaa gcc gag gat caa aaa gaa gaa gat cgc cgt aac tac   960
Ala Lys Lys Lys Ala Glu Asp Gln Lys Glu Glu Asp Arg Arg Asn Tyr
305                 310                 315                 320
 
cca acc aat act tac aaa acg ctt gaa ctt gaa att gct gag tcc gat   1008
Pro Thr Asn Thr Tyr Lys Thr Leu Glu Leu Glu Ile Ala Glu Ser Asp
                325                 330                 335
 
gtg gaa gtt aaa aaa gcg gag ctt gaa cta gta aaa gag gaa gct aag   1056
Val Glu Val Lys Lys Ala Glu Leu Glu Leu Val Lys Glu Glu Ala Lys
            340                 345                 350
 
gaa cct cga aac gag gaa aaa gtt aag caa gca aaa gcg gaa gtt gag   1104
Glu Pro Arg Asn Glu Glu Lys Val Lys Gln Ala Lys Ala Glu Val Glu
        355                 360                 365
 
agt aaa aaa gct gag gct aca agg tta gaa aaa atc aag aca gat cgt   1152
Ser Lys Lys Ala Glu Ala Thr Arg Leu Glu Lys Ile Lys Thr Asp Arg
    370                 375                 380
 
aaa aaa gca gaa gaa gaa gct aaa cga aaa gca gca gaa gaa gat aaa   1200
Lys Lys Ala Glu Glu Glu Ala Lys Arg Lys Ala Ala Glu Glu Asp Lys
385                 390                 395                 400
 
gtt aaa gaa aaa cca gct gaa caa cca caa cca gcg ccg gct cca aaa   1248
Val Lys Glu Lys Pro Ala Glu Gln Pro Gln Pro Ala Pro Ala Pro Lys
                405                 410                 415
 
gca gaa aaa cca gct cca gct cca aaa cca gag aat cca gct gaa caa   1296
Ala Glu Lys Pro Ala Pro Ala Pro Lys Pro Glu Asn Pro Ala Glu Gln
            420                 425                 430
 
cca aaa gca gaa aaa cca gct gat caa caa gct gaa gaa gac tat gct   1344
Pro Lys Ala Glu Lys Pro Ala Asp Gln Gln Ala Glu Glu Asp Tyr Ala
        435                 440                 445
 
cgt aga tca gaa gaa gaa tat aat cgc ttg act caa cag caa ccg cca   1392
Arg Arg Ser Glu Glu Glu Tyr Asn Arg Leu Thr Gln Gln Gln Pro Pro
    450                 455                 460
 
aaa act gaa aaa cca gca caa cca                                   1416
Lys Thr Glu Lys Pro Ala Gln Pro
465                 470
 

<210> 5
<211> 115
<212> PRT
<213> Streptococcus pneumoniae

<400> 5
Pro Glu Lys Lys Val Ala Glu Ala Glu Lys Lys Val Glu Glu Ala Lys
 1               5                  10                  15      
Lys Lys Ala Glu Asp Gln Lys Glu Glu Asp Arg Arg Asn Tyr Pro Thr
            20                  25                  30          
Asn Thr Tyr Lys Thr Leu Glu Leu Glu Ile Ala Glu Ser Asp Val Glu
        35                  40                  45              
Val Lys Lys Ala Glu Leu Glu Leu Val Lys Glu Glu Ala Lys Glu Pro
    50                  55                  60                  
Arg Asn Glu Glu Lys Val Lys Gln Ala Lys Ala Glu Val Glu Ser Lys
65                  70                  75                  80  
Lys Ala Glu Ala Thr Arg Leu Glu Lys Ile Lys Thr Asp Arg Lys Lys
                85                  90                  95      
Ala Glu Glu Glu Ala Lys Arg Lys Ala Ala Glu Glu Asp Lys Val Lys
            100                 105                 110         
Glu Lys Pro
        115 


<210> 6
<211> 345
<212> DNA
<213> Streptococcus pneumoniae

<220> 
<221> misc_feature   
<222> (0)...(0)
<223> Nucleotide sequence encoding the R2 polypeptide
      set forth in SEQ ID NO:5 corresponding to amino
      acid residues 329-443 of the CbpA protein from
      Streptococcus pneumoniae Tigr4 strain

<221> CDS            
<222> (1)...(345)

<400> 6
cca gaa aaa aag gta gca gaa gct gag aag aag gtt gaa gaa gct aag   48
Pro Glu Lys Lys Val Ala Glu Ala Glu Lys Lys Val Glu Glu Ala Lys
 1               5                   10                  15
 
aaa aaa gcc gag gat caa aaa gaa gaa gat cgc cgt aac tac cca acc   96
Lys Lys Ala Glu Asp Gln Lys Glu Glu Asp Arg Arg Asn Tyr Pro Thr
             20                  25                  30
 
aat act tac aaa acg ctt gaa ctt gaa att gct gag tcc gat gtg gaa   144
Asn Thr Tyr Lys Thr Leu Glu Leu Glu Ile Ala Glu Ser Asp Val Glu
         35                  40                  45
 
gtt aaa aaa gcg gag ctt gaa cta gta aaa gag gaa gct aag gaa cct   192
Val Lys Lys Ala Glu Leu Glu Leu Val Lys Glu Glu Ala Lys Glu Pro
     50                  55                  60
 
cga aac gag gaa aaa gtt aag caa gca aaa gcg gaa gtt gag agt aaa   240
Arg Asn Glu Glu Lys Val Lys Gln Ala Lys Ala Glu Val Glu Ser Lys
 65                  70                  75                  80
 
aaa gct gag gct aca agg tta gaa aaa atc aag aca gat cgt aaa aaa   288
Lys Ala Glu Ala Thr Arg Leu Glu Lys Ile Lys Thr Asp Arg Lys Lys
                 85                  90                  95
 
gca gaa gaa gaa gct aaa cga aaa gca gca gaa gaa gat aaa gtt aaa   336
Ala Glu Glu Glu Ala Lys Arg Lys Ala Ala Glu Glu Asp Lys Val Lys
            100                 105                 110
 
gaa aaa cca                                                       345
Glu Lys Pro
        115
 

<210> 7
<211> 63
<212> PRT
<213> Streptococcus pneumoniae

<400> 7
Pro Glu Lys Lys Val Ala Glu Ala Glu Lys Lys Val Glu Glu Ala Lys
 1               5                  10                  15      
Lys Lys Ala Glu Asp Gln Lys Glu Glu Asp Arg Arg Asn Tyr Pro Thr
            20                  25                  30          
Asn Thr Tyr Lys Thr Leu Glu Leu Glu Ile Ala Glu Ser Asp Val Glu
        35                  40                  45              
Val Lys Lys Ala Glu Leu Glu Leu Val Lys Glu Glu Ala Lys Glu
    50                  55                  60              


<210> 8
<211> 189
<212> DNA
<213> Streptococcus pneumoniae

<220> 
<221> misc_feature   
<222> (0)...(0)
<223> Nucleotide sequence encoding the R21 polypeptide
      set forth in SEQ ID NO:7 corresponding to residues
      329-391 of the CbpA protein from Streptococcus
      pneumoniae Tigr4 strain

<221> CDS            
<222> (1)...(189)

<400> 8
cca gaa aaa aag gta gca gaa gct gag aag aag gtt gaa gaa gct aag   48
Pro Glu Lys Lys Val Ala Glu Ala Glu Lys Lys Val Glu Glu Ala Lys
 1               5                   10                  15
 
aaa aaa gcc gag gat caa aaa gaa gaa gat cgc cgt aac tac cca acc   96
Lys Lys Ala Glu Asp Gln Lys Glu Glu Asp Arg Arg Asn Tyr Pro Thr
             20                  25                  30
 
aat act tac aaa acg ctt gaa ctt gaa att gct gag tcc gat gtg gaa   144
Asn Thr Tyr Lys Thr Leu Glu Leu Glu Ile Ala Glu Ser Asp Val Glu
         35                  40                  45
 
gtt aaa aaa gcg gag ctt gaa cta gta aaa gag gaa gct aag gaa       189
Val Lys Lys Ala Glu Leu Glu Leu Val Lys Glu Glu Ala Lys Glu
     50                  55                  60
 


<210> 9
<211> 83
<212> PRT
<213> Streptococcus pneumoniae

<400> 9
Asn Thr Tyr Lys Thr Leu Glu Leu Glu Ile Ala Glu Ser Asp Val Glu
 1               5                  10                  15      
Val Lys Lys Ala Glu Leu Glu Leu Val Lys Glu Glu Ala Lys Glu Pro
            20                  25                  30          
Arg Asn Glu Glu Lys Val Lys Gln Ala Lys Ala Glu Val Glu Ser Lys
        35                  40                  45              
Lys Ala Glu Ala Thr Arg Leu Glu Lys Ile Lys Thr Asp Arg Lys Lys
    50                  55                  60                  
Ala Glu Glu Glu Ala Lys Arg Lys Ala Ala Glu Glu Asp Lys Val Lys
65                  70                  75                  80  
Glu Lys Pro
            


<210> 10
<211> 249
<212> DNA
<213> Streptococcus pneumoniae

<220> 
<221> misc_feature   
<222> (0)...(0)
<223> Nucleotide sequence encoding the R22 polypeptide
      set forth in SEQ ID NO:9 corresponding to residues
      361-443 of the CbpA protein from Streptococcus
      pneumoniae Tigr4 strain

<221> CDS            
<222> (1)...(249)

<400> 10
aat act tac aaa acg ctt gaa ctt gaa att gct gag tcc gat gtg gaa   48
Asn Thr Tyr Lys Thr Leu Glu Leu Glu Ile Ala Glu Ser Asp Val Glu
 1               5                   10                  15
 
gtt aaa aaa gcg gag ctt gaa cta gta aaa gag gaa gct aag gaa cct   96
Val Lys Lys Ala Glu Leu Glu Leu Val Lys Glu Glu Ala Lys Glu Pro
             20                  25                  30
 
cga aac gag gaa aaa gtt aag caa gca aaa gcg gaa gtt gag agt aaa   144
Arg Asn Glu Glu Lys Val Lys Gln Ala Lys Ala Glu Val Glu Ser Lys
         35                  40                  45
 
aaa gct gag gct aca agg tta gaa aaa atc aag aca gat cgt aaa aaa   192
Lys Ala Glu Ala Thr Arg Leu Glu Lys Ile Lys Thr Asp Arg Lys Lys
     50                  55                  60
 
gca gaa gaa gaa gct aaa cga aaa gca gca gaa gaa gat aaa gtt aaa   240
Ala Glu Glu Glu Ala Lys Arg Lys Ala Ala Glu Glu Asp Lys Val Lys
 65                  70                  75                  80
 
gaa aaa cca                                                       249
Glu Lys Pro

 

<210> 11
<211> 6
<212> PRT
<213> Streptococcus pneumoniae

<400> 11
Arg Arg Asn Tyr Pro Thr
 1               5      


<210> 12
<211> 18
<212> DNA
<213> Streptococcus pneumoniae

<220> 
<221> misc_feature   
<222> (0)...(0)
<223> Nucleotide sequence encoding the amino acid
      sequence for the putative pIgR binding site of
      CbpA set forth in SEQ ID NO:11

<221> CDS            
<222> (1)...(18)

<400> 12
cgc cgt aac tac cca acc                                           18
Arg Arg Asn Tyr Pro Thr
 1               5
 

<210> 13
<211> 7
<212> PRT
<213> Streptococcus pneumoniae

<400> 13
Glu Pro Arg Asn Glu Glu Lys
 1               5          


<210> 14
<211> 21
<212> DNA
<213> Streptococcus pneumoniae

<220> 
<221> misc_feature   
<222> (0)...(0)
<223> Nucleotide sequence encoding the amino acid
      sequence for the putative laminin receptor binding
      site of CbpA set forth in SEQ ID NO:13
      

<221> CDS            
<222> (1)...(21)

<400> 14
gaa cct cga aac gag gaa aaa                                       21
Glu Pro Arg Asn Glu Glu Lys
 1               5
 

<210> 15
<211> 62
<212> PRT
<213> Artificial Sequence

<220> 
<223> Amino acid sequence for the CbpA-mR21/Cys
      polypeptide construct

<400> 15
Pro Glu Lys Lys Cys Ala Glu Ala Glu Lys Lys Val Glu Glu Ala Lys
 1               5                  10                  15      
Lys Lys Ala Glu Asp Gln Lys Glu Glu Asp Arg Arg Asn Tyr Pro Thr
            20                  25                  30          
Asn Thr Tyr Lys Thr Leu Glu Leu Glu Ile Ala Glu Ser Asp Val Glu
        35                  40                  45              
Val Lys Lys Ala Glu Leu Glu Leu Cys Lys Glu Glu Ala Lys
    50                  55                  60          


<210> 16
<211> 192
<212> DNA
<213> Artificial Sequence

<220> 
<223> Nucleotide sequence encoding the CbpA-mR21/Cys
      polypeptide construct set forth in SEQ ID NO:15

<400> 16
atgccagaaa aaaagtgtgc agaagctgag aagaaggttg aagaagctaa gaaaaaagcc 60
gaggatcaaa aagaagaaga tcgccgtaac tacccaacca atacttacaa aacgcttgaa 120
cttgaaattg ctgagtccga tgtggaagtt aaaaaagcgg agcttgaact agtatgtgag 180
gaagctaagg aa                                                     192

<210> 17
<211> 83
<212> PRT
<213> Artificial Sequence

<220> 
<223> Amino acid sequence for the CbpA-mR22/Cys
      polypeptide construct

<400> 17
Asn Thr Tyr Cys Thr Leu Glu Leu Glu Ile Ala Glu Ser Asp Val Glu
 1               5                  10                  15      
Val Lys Lys Ala Glu Leu Glu Leu Val Lys Glu Glu Ala Lys Glu Pro
            20                  25                  30          
Arg Asn Glu Glu Lys Val Lys Gln Ala Lys Ala Glu Val Glu Ser Lys
        35                  40                  45              
Lys Ala Glu Ala Thr Arg Leu Glu Lys Ile Lys Thr Asp Arg Lys Lys
    50                  55                  60                  
Ala Glu Glu Glu Ala Lys Arg Lys Ala Ala Glu Glu Asp Lys Cys Lys
65                  70                  75                  80  
Glu Lys Pro
            


<210> 18
<211> 252
<212> DNA
<213> Artificial Sequence

<220> 
<223> Nucleotide sequence encoding the CbpA-mR22/Cys
      polypeptide construct set forth in SEQ ID NO:17

<400> 18
atgaatactt actgtacgct tgaacttgaa attgctgagt ccgatgtgga agttaaaaaa 60
gcggagcttg aactagtaaa agaggaagct aaggaacctc gaaacgagga aaaagttaag 120
caagcaaaag cggaagttga gagtaaaaaa gctgaggcta caaggttaga aaaaatcaag 180
acagatcgta aaaaagcaga agaagaagct aaacgaaaag cagcagaaga agataaatgt 240
aaagaaaaac ca                                                     252

<210> 19
<211> 30
<212> PRT
<213> Streptococcus pneumoniae

<220> 
<221> PEPTIDE        
<222> (1)...(30)
<223> Amino acid sequence for the NYPTs polypeptide
      construct

<400> 19
Ala Lys Lys Lys Ala Glu Asp Gln Lys Glu Glu Asp Arg Arg Asn Tyr
 1               5                  10                  15      
Pro Thr Asn Thr Tyr Lys Thr Leu Glu Leu Glu Ile Ala Glu
            20                  25                  30  


<210> 20
<211> 90
<212> DNA
<213> Streptococcus pneumoniae

<220> 
<221> misc_feature   
<222> (0)...(0)
<223> Nucleotide sequence encoding the NYPTs polypeptide
      construct set forth in SEQ ID NO:19

<221> CDS            
<222> (1)...(90)

<400> 20
gct aag aaa aaa gcc gag gat caa aaa gaa gaa gat cgc cgt aac tac   48
Ala Lys Lys Lys Ala Glu Asp Gln Lys Glu Glu Asp Arg Arg Asn Tyr
 1               5                   10                  15
 
cca acc aat act tac aaa acg ctt gaa ctt gaa att gct gag           90
Pro Thr Asn Thr Tyr Lys Thr Leu Glu Leu Glu Ile Ala Glu
             20                  25                  30
 


<210> 21
<211> 30
<212> PRT
<213> Artificial Sequence

<220> 
<223> Amino acid sequence for the NYPTsCys polypeptide
      construct

<400> 21
Ala Cys Lys Lys Ala Glu Asp Gln Lys Glu Glu Asp Arg Arg Asn Tyr
 1               5                  10                  15      
Pro Thr Asn Thr Tyr Lys Thr Leu Glu Leu Glu Cys Ala Glu
            20                  25                  30  


<210> 22
<211> 90
<212> DNA
<213> Artificial Sequence

<220> 
<223> Nucleotide sequence encoding the NYPTsCys
      polypeptide construct set forth in SEQ ID NO:21

<400> 22
gcttgtaaaa aagccgagga tcaaaaagaa gaagatcgcc gtaactaccc aaccaatact 60
tacaaaacgc ttgaacttga atgtgctgag                                  90

<210> 23
<211> 46
<212> PRT
<213> Streptococcus pneumoniae

<220> 
<221> PEPTIDE        
<222> (1)...(46)
<223> Amino acid sequence for the NYPT1 polypeptide
      construct

<400> 23
Gly Glu Lys Lys Val Ala Glu Ala Lys Lys Lys Ala Glu Asp Gln Lys
 1               5                  10                  15      
Glu Glu Asp Arg Arg Asn Tyr Pro Thr Asn Thr Tyr Lys Thr Leu Glu
            20                  25                  30          
Leu Glu Ile Ala Glu Ser Asp Val Glu Val Lys Lys Ala Glu
        35                  40                  45      


<210> 24
<211> 159
<212> DNA
<213> Streptococcus pneumoniae

<220> 
<221> misc_feature   
<222> (0)...(0)
<223> Nucleotide sequence encoding the NYPT1 polypeptide
      construct set forth in SEQ ID NO:23

<221> CDS            
<222> (1)...(159)

<400> 24
ggg gaa aaa aag gta gca gaa gct gag aag aag gtt gaa gaa gct aag   48
Gly Glu Lys Lys Val Ala Glu Ala Glu Lys Lys Val Glu Glu Ala Lys
 1               5                   10                  15
 
aaa aaa gcc gag gat caa aaa gaa gaa gat cgc cgt aac tac cca acc   96
Lys Lys Ala Glu Asp Gln Lys Glu Glu Asp Arg Arg Asn Tyr Pro Thr
             20                  25                  30
 
aat act tac aaa acg ctt gaa ctt gaa att gct gag tcc gat gtg gaa   144
Asn Thr Tyr Lys Thr Leu Glu Leu Glu Ile Ala Glu Ser Asp Val Glu
         35                  40                  45
 
gtt aaa aaa gcg gag                                               159
Val Lys Lys Ala Glu
     50
 

<210> 25
<211> 45
<212> PRT
<213> Artificial Sequence

<220> 
<223> Amino acid sequence for the NYPT1Cys polypeptide
      construct

<400> 25
Glu Lys Lys Val Ala Glu Ala Cys Lys Lys Ala Glu Asp Gln Lys Glu
 1               5                  10                  15      
Glu Asp Arg Arg Asn Tyr Pro Thr Asn Thr Tyr Lys Thr Leu Glu Leu
            20                  25                  30          
Glu Cys Ala Glu Ser Asp Val Glu Val Lys Lys Ala Glu
        35                  40                  45  


<210> 26
<211> 159
<212> DNA
<213> Artificial Sequence

<220> 
<223> Nucleotide sequence encoding the NYPT1Cys
      polypeptide construct set forth in SEQ ID NO:25

<400> 26
ggggaaaaaa aggtagcaga atgtgagaag aaggttgaag aagctaagaa aaaagccgag 60
gatcaaaaag aagaagatcg ccgtaactac ccaaccaata cttacaaaac gcttgaactt 120
gaatgtgctg agtccgatgt ggaagttaaa aaagcggag                        159

<210> 27
<211> 30
<212> PRT
<213> Streptococcus pneumoniae

<220> 
<221> PEPTIDE        
<222> (1)...(30)
<223> Amino acid sequence for the NEEKs polypeptide
      construct

<400> 27
Lys Ala Glu Leu Glu Leu Val Lys Glu Glu Ala Lys Glu Pro Arg Asn
 1               5                  10                  15      
Glu Glu Lys Val Lys Gln Ala Lys Ala Glu Val Glu Ser Lys
            20                  25                  30  


<210> 28
<211> 90
<212> DNA
<213> Streptococcus pneumoniae

<220> 
<221> misc_feature   
<222> (0)...(0)
<223> Nucleotide sequence encoding the NEEKs polypeptide
      construct set forth in SEQ ID NO:27

<221> CDS            
<222> (1)...(90)

<400> 28
aaa gcg gag ctt gaa cta gta aaa gag gaa gct aag gaa cct cga aac   48
Lys Ala Glu Leu Glu Leu Val Lys Glu Glu Ala Lys Glu Pro Arg Asn
 1               5                   10                  15
 
gag gaa aaa gtt aag caa gca aaa gcg gaa gtt gag agt aaa           90
Glu Glu Lys Val Lys Gln Ala Lys Ala Glu Val Glu Ser Lys
             20                  25                  30
 


<210> 29
<211> 30
<212> PRT
<213> Artificial Sequence

<220> 
<223> Amino acid sequence for the NEEKsCys polypeptide
      construct

<400> 29
Lys Cys Glu Leu Glu Leu Val Lys Glu Glu Ala Lys Glu Pro Arg Asn
 1               5                  10                  15      
Glu Glu Lys Val Lys Gln Ala Lys Ala Glu Cys Glu Ser Lys
            20                  25                  30  


<210> 30
<211> 90
<212> DNA
<213> Artificial Sequence

<220> 
<223> Nucleotide sequence encoding the NEEKsCys
      polypeptide construct set forth in SEQ ID NO:29

<400> 30
aaatgtgagc ttgaactagt aaaagaggaa gctaaggaac ctcgaaacga ggaaaaagtt 60
aagcaagcaa aagcggaatg tgagagtaaa                                  90

<210> 31
<211> 46
<212> PRT
<213> Streptococcus pneumoniae

<220> 
<221> PEPTIDE        
<222> (1)...(46)
<223> Amino acid sequence for the NEEK1 polypeptide
      construct

<400> 31
Gly Glu Ser Asp Val Glu Val Lys Lys Ala Glu Leu Glu Leu Val Lys
 1               5                  10                  15      
Glu Glu Ala Lys Glu Pro Arg Asn Glu Glu Lys Val Lys Gln Ala Lys
            20                  25                  30          
Ala Glu Val Glu Ser Lys Lys Ala Glu Ala Thr Arg Leu Glu
        35                  40                  45      


<210> 32
<211> 135
<212> DNA
<213> Streptococcus pneumoniae

<220> 
<221> misc_feature   
<222> (0)...(0)
<223> Nucleotide sequence encoding the NEEK1 polypeptide
      construct set forth in SEQ ID NO:31

<221> CDS            
<222> (1)...(135)

<400> 32
gag tcc gat gtg gaa gtt aaa aaa gcg gag ctt gaa cta gta aaa gag   48
Glu Ser Asp Val Glu Val Lys Lys Ala Glu Leu Glu Leu Val Lys Glu
 1               5                   10                  15
 
gaa gct aag gaa cct cga aac gag gaa aaa gtt aag caa gca aaa gcg   96
Glu Ala Lys Glu Pro Arg Asn Glu Glu Lys Val Lys Gln Ala Lys Ala
             20                  25                  30
 
gaa gtt gag agt aaa aaa gct gag gct aca agg tta gaa               135
Glu Val Glu Ser Lys Lys Ala Glu Ala Thr Arg Leu Glu
         35                  40                  45
 

<210> 33
<211> 45
<212> PRT
<213> Artificial Sequence

<220> 
<223> Amino acid sequence for the NEEK1Cys polypeptide
      construct

<400> 33
Glu Ser Asp Val Glu Val Lys Cys Ala Glu Leu Glu Leu Val Lys Glu
 1               5                  10                  15      
Glu Ala Lys Glu Pro Arg Asn Glu Glu Lys Val Lys Gln Ala Lys Ala
            20                  25                  30          
Glu Cys Glu Ser Lys Lys Ala Glu Ala Thr Arg Leu Glu
        35                  40                  45  


<210> 34
<211> 138
<212> DNA
<213> Artificial Sequence

<220> 
<223> Nucleotide sequence encoding the NEEK1Cys
      polypeptide construct set forth in SEQ ID NO:33

<400> 34
ggggagtccg atgtggaagt taaatgtgcg gagcttgaac tagtaaaaga ggaagctaag 60
gaacctcgaa acgaggaaaa agttaagcaa gcaaaagcgg aatgtgagag taaaaaagct 120
gaggctacaa ggttagaa                                               138

<210> 35
<211> 30
<212> PRT
<213> Streptococcus pneumoniae

<220> 
<221> PEPTIDE        
<222> (1)...(30)
<223> Amino acid sequence for the NEEKnterm polypeptide
      construct

<400> 35
Leu Glu Ile Ala Glu Ser Asp Val Glu Val Lys Lys Ala Glu Leu Glu
 1               5                  10                  15      
Leu Val Lys Glu Glu Ala Lys Glu Pro Arg Asn Glu Glu Lys
            20                  25                  30  


<210> 36
<211> 90
<212> DNA
<213> Streptococcus pneumoniae

<220> 
<221> misc_feature   
<222> (0)...(0)
<223> Nucleotide sequence encoding the NEEKnterm
      polypeptide construct set forth in SEQ ID NO:35

<221> CDS            
<222> (1)...(90)

<400> 36
ctt gaa att gct gag tcc gat gtg gaa gtt aaa aaa gcg gag ctt gaa   48
Leu Glu Ile Ala Glu Ser Asp Val Glu Val Lys Lys Ala Glu Leu Glu
 1               5                   10                  15
 
cta gta aaa gag gaa gct aag gaa cct cga aac gag gaa aaa           90
Leu Val Lys Glu Glu Ala Lys Glu Pro Arg Asn Glu Glu Lys
             20                  25                  30
 


<210> 37
<211> 30
<212> PRT
<213> Streptococcus pneumoniae

<220> 
<221> PEPTIDE        
<222> (1)...(30)
<223> Amino acid sequence for the NEEKcterm polypeptide
      construct

<400> 37
Glu Pro Arg Asn Glu Glu Lys Val Lys Gln Ala Lys Ala Glu Val Glu
 1               5                  10                  15      
Ser Lys Lys Ala Glu Ala Thr Arg Leu Glu Lys Ile Lys Thr
            20                  25                  30  


<210> 38
<211> 90
<212> DNA
<213> Streptococcus pneumoniae

<220> 
<221> misc_feature   
<222> (0)...(0)
<223> Nucleotide sequence encoding the NEEKcterm
      polypeptide construct set forth in SEQ ID NO:37

<221> CDS            
<222> (1)...(90)

<400> 38
gaa cct cga aac gag gaa aaa gtt aag caa gca aaa gcg gaa gtt gag   48
Glu Pro Arg Asn Glu Glu Lys Val Lys Gln Ala Lys Ala Glu Val Glu
 1               5                   10                  15
 
agt aaa aaa gct gag gct aca agg tta gaa aaa atc aag aca           90
Ser Lys Lys Ala Glu Ala Thr Arg Leu Glu Lys Ile Lys Thr
             20                  25                  30
 


<210> 39
<211> 30
<212> PRT
<213> Streptococcus pneumoniae

<220> 
<221> PEPTIDE        
<222> (1)...(30)
<223> Amino acid sequence for the R2cterm polypeptide
      construct

<400> 39
Arg Leu Glu Lys Ile Lys Thr Asp Arg Lys Lys Ala Glu Glu Glu Ala
 1               5                  10                  15      
Lys Arg Lys Ala Ala Glu Glu Asp Lys Val Lys Glu Lys Pro
            20                  25                  30  


<210> 40
<211> 90
<212> DNA
<213> Streptococcus pneumoniae

<220> 
<221> misc_feature   
<222> (0)...(0)
<223> Nucleotide sequence encoding the R2cterm
      polypeptide construct set forth in SEQ ID NO:39

<221> CDS            
<222> (1)...(90)

<400> 40
agg tta gaa aaa atc aag aca gat cgt aaa aaa gca gaa gaa gaa gct   48
Arg Leu Glu Lys Ile Lys Thr Asp Arg Lys Lys Ala Glu Glu Glu Ala
 1               5                   10                  15
 
aaa cga aaa gca gca gaa gaa gat aaa gtt aaa gaa aaa cca           90
Lys Arg Lys Ala Ala Glu Glu Asp Lys Val Lys Glu Lys Pro
             20                  25                  30
 


<210> 41
<211> 45
<212> PRT
<213> Artificial Sequence

<220> 
<223> Amino acid sequence for the TCENYPTsCys
      polypeptide construct

<400> 41
Gln Tyr Ile Lys Ala Asn Ser Lys Phe Ile Gly Ile Thr Gly Gly Ala
 1               5                  10                  15      
Cys Lys Lys Ala Glu Asp Gln Lys Glu Glu Asp Arg Arg Asn Tyr Pro
            20                  25                  30          
Thr Asn Thr Tyr Lys Thr Leu Glu Leu Glu Cys Ala Glu
        35                  40                  45  


<210> 42
<211> 135
<212> DNA
<213> Artificial Sequence

<220> 
<223> Nucleotide sequence encoding the TCENYPTsCys
      polypeptide construct set forth in SEQ ID NO:41

<400> 42
cagtatataa aagcgaattc taaattcatc gggatcacgg gcggggcttg taaaaaagcc 60
gaggatcaaa aagaagaaga tcgccgtaac tacccaacca atacttacaa aacgcttgaa 120
cttgaatgtg ctgag                                                  135

<210> 43
<211> 45
<212> PRT
<213> Artificial Sequence

<220> 
<223> Amino acid sequence for the TCENEEKsCys polypeptide
      construct

<400> 43
Gln Tyr Ile Lys Ala Asn Ser Lys Phe Ile Gly Ile Thr Gly Gly Lys
 1               5                  10                  15      
Cys Glu Leu Glu Leu Val Lys Glu Glu Ala Lys Glu Pro Arg Asn Glu
            20                  25                  30          
Glu Lys Val Lys Gln Ala Lys Ala Glu Cys Glu Ser Lys
        35                  40                  45  


<210> 44
<211> 135
<212> DNA
<213> Artificial Sequence

<220> 
<223> Nucleotide sequence encoding the TCENEEKsCys
      polypeptide construct set forth in SEQ ID NO:43

<400> 44
cagtatataa aagcgaattc taaattcatc gggatcacgg gcgggaaatg tgagcttgaa 60
ctagtaaaag aggaagctaa ggaacctcga aacgaggaaa aagttaagca agcaaaagcg 120
gaatgtgaga gtaaa                                                  135

<210> 45
<211> 41
<212> DNA
<213> Artificial Sequence

<220> 
<223> PCR primer for mutagenesis of V333C in the
      CbpA-mR21/Cys polypeptide construct

<400> 45
cctgaaacca gaaaaaaagt gtgcagaagc tgagaagaag g                     41

<210> 46
<211> 38
<212> DNA
<213> Artificial Sequence

<220> 
<223> PCR primer for mutagenesis of K386C in the
      CbpA-mR21/Cys polypeptide construct

<400> 46
gcggagcttg aactagtatg tgaggaagct aaggaacc                         38

<210> 47
<211> 40
<212> DNA
<213> Artificial Sequence

<220> 
<223> PCR primer for mutagenesis of K364C in the
      CbpA-mR22/Cys polypeptide construct

<400> 47
catatgaata cttactgcac gcttgaactt gaaattgctg                       40

<210> 48
<211> 42
<212> DNA
<213> Artificial Sequence

<220> 
<223> PCR primer for mutagenesis of V439C in the
      CbpA-mR22/Cys polypeptide construct

<400> 48
gcagcagaag aagataaatg caaagaaaaa ccataagaat tc                    42

<210> 49
<211> 15
<212> PRT
<213> Artificial Sequence

<220> 
<223> Amino acid sequence for the minimal functional
      T-cell epitope further comprising a
      glycine-glycine linker

<400> 49
Gln Tyr Ile Lys Ala Asn Ser Lys Phe Ile Gly Ile Thr Gly Gly
 1               5                  10                  15  


<210> 50
<211> 20
<212> PRT
<213> Homo sapiens

<400> 50
Ile Pro Cys Asn Asn Lys Gly Ala His Ser Val Gly Leu Met Trp Trp
 1               5                  10                  15      
Met Leu Ala Arg
            20  


<210> 51
<211> 20
<212> PRT
<213> Homo sapiens

<400> 51
Gln Phe Pro Thr Glu Asp Trp Ser Ala Gln Pro Ala Thr Glu Asp Trp
 1               5                  10                  15      
Ser Ala Ala Pro
            20  


<210> 52
<211> 295
<212> PRT
<213> Homo sapiens

<400> 52
Met Ser Gly Ala Leu Asp Val Leu Gln Met Lys Glu Glu Asp Val Leu
 1               5                  10                  15      
Lys Phe Leu Ala Ala Gly Thr His Leu Gly Gly Thr Asn Leu Asp Phe
            20                  25                  30          
Gln Met Glu Gln Tyr Ile Tyr Lys Arg Lys Ser Asp Gly Ile Tyr Ile
        35                  40                  45              
Ile Asn Leu Lys Arg Thr Trp Glu Lys Leu Leu Leu Ala Ala Arg Ala
    50                  55                  60                  
Ile Val Ala Ile Glu Asn Pro Ala Asp Val Ser Val Ile Ser Ser Arg
65                  70                  75                  80  
Asn Thr Gly Gln Arg Ala Val Leu Lys Phe Ala Ala Ala Thr Gly Ala
                85                  90                  95      
Thr Pro Ile Ala Gly Arg Phe Thr Pro Gly Thr Phe Thr Asn Gln Ile
            100                 105                 110         
Gln Ala Ala Phe Arg Glu Pro Arg Leu Leu Val Val Thr Asp Pro Arg
        115                 120                 125             
Ala Asp His Gln Pro Leu Thr Glu Ala Ser Tyr Val Asn Leu Pro Thr
    130                 135                 140                 
Ile Ala Leu Cys Asn Thr Asp Ser Pro Leu Arg Tyr Val Asp Ile Ala
145                 150                 155                 160 
Ile Pro Cys Asn Asn Lys Gly Ala His Ser Val Gly Leu Met Trp Trp
                165                 170                 175     
Met Leu Ala Arg Glu Val Leu Arg Met Arg Gly Thr Ile Ser Arg Glu
            180                 185                 190         
His Pro Trp Glu Val Met Pro Asp Leu Tyr Phe Tyr Arg Asp Pro Glu
        195                 200                 205             
Glu Ile Glu Lys Glu Glu Gln Ala Ala Ala Glu Lys Ala Val Thr Lys
    210                 215                 220                 
Glu Glu Phe Gln Gly Glu Trp Thr Ala Pro Ala Pro Glu Phe Thr Ala
225                 230                 235                 240 
Thr Gln Pro Glu Val Ala Asp Trp Ser Glu Gly Val Gln Val Pro Ser
                245                 250                 255     
Val Pro Ile Gln Gln Phe Pro Thr Glu Asp Trp Ser Ala Gln Pro Ala
            260                 265                 270         
Thr Glu Asp Trp Ser Ala Ala Pro Thr Ala Gln Ala Thr Glu Trp Val
        275                 280                 285             
Gly Ala Thr Thr Asp Trp Ser
    290                 295 


<210> 53
<211> 20
<212> PRT
<213> Artificial Sequence

<220> 
<223> Scrambled control peptide of amino acid residues
      161-180 of the laminin receptor (SEQ ID NO:52)

<400> 53
Ala Trp Val Gly Cys Leu Met Ser Lys Pro Arg Ile His Trp Asn Met
 1               5                  10                  15      
Ala Leu Asn Gly
            20  


<210> 54
<211> 18
<212> PRT
<213> Artificial Sequence

<220> 
<223> Scrambled negative control peptide of amino acid
      residues 263-282 of the laminin receptor (SEQ ID
      NO:52)

<400> 54
Asp Pro Trp Ala Ser Ala Pro Ser Thr Ala Thr Ala Asp Glu Pro Trp
 1               5                  10                  15      
Ala Thr
        


<210> 55
<211> 5
<212> PRT
<213> Artificial Sequence

<220> 
<223> CbpA R2 protein negative control peptide

<400> 55
Leu Ser Glu Ile Lys
 1               5  


<210> 56
<211> 13
<212> PRT
<213> Artificial Sequence

<220> 
<223> Amino acid sequence for tt830 T cell epitope

<400> 56
Gln Tyr Ile Lys Ala Asn Ser Lys Phe Ile Gly Ile Thr
 1               5                  10              


<210> 57
<211> 15
<212> PRT
<213> Artificial Sequence

<220> 
<223> Amino acid sequence for F421 T cell epitope

<400> 57
Pro Val Val Glu Val Asn Gly Val Thr Ile Gln Val Gly Ser Arg
 1               5                  10                  15  


<210> 58
<211> 6
<212> PRT
<213> Artificial Sequence

<220> 
<223> Palindromic peptide sequence within residues
      161-180 of the laminin receptor (SEQ ID NO:52)

<400> 58
Leu Met Trp Trp Met Leu
 1               5      


<210> 59
<211> 5
<212> PRT
<213> Artificial Sequence

<220> 
<223> Laminin receptor binding peptide

<400> 59
Tyr Ile Gly Ser Arg
 1               5  


<210> 60
<211> 6
<212> PRT
<213> Artificial Sequence

<220> 
<223> Laminin receptor binding peptide

<400> 60
Leu Gly Thr Ile Pro Gly
 1               5      


<210> 61
<211> 6
<212> PRT
<213> Artificial Sequence

<220> 
<223> Laminin receptor-binding peptide in elastin

<400> 61
Val Gly Val Ala Pro Gly
 1               5      


<210> 62
<211> 5
<212> PRT
<213> Streptococcus pneumoniae

<400> 62
Arg Asn Tyr Pro Thr
 1               5  


<210> 63
<211> 5
<212> PRT
<213> Artificial Sequence

<220> 
<223> Isogenic mutant substitution for RNYPT sequence of
      S. pneumoniae

<400> 63
Arg Asn Gly Pro Thr
 1               5  


<210> 64
<211> 7
<212> PRT
<213> Streptococcus pneumoniae

<400> 64
Glu Pro Arg Asp Glu Gln Lys
 1               5          

<210> 65
<211> 28
<212> PRT
<213> Artificial Sequence

<220> 
<223> T cell epitope

<400> 65
Gln Tyr Ile Lys Ala Asn Ser Lys Phe Ile Gly Ile Thr Gln Tyr Ile
 1               5                  10                  15      
Lys Ala Asn Ser Lys Phe Ile Gly Ile Thr Gly Gly
            20                  25              


<210> 66
<211> 17
<212> PRT
<213> Artificial Sequence

<220> 
<223> T cell epitope

<400> 66
Pro Val Val Glu Val Asn Gly Val Thr Ile Gln Val Gly Ser Arg Gly
 1               5                  10                  15      
Gly
    


<210> 67
<211> 45
<212> PRT
<213> Artificial Sequence

<220> 
<223> CbpA-R21 sequence with incorporated cysteine
      residues and linked T cell epitope

<400> 67
Ala Cys Lys Lys Ala Glu Asp Gln Lys Glu Glu Asp Arg Arg Asn Tyr
 1               5                  10                  15      
Pro Thr Asn Thr Tyr Lys Thr Leu Glu Leu Glu Cys Ala Glu Gly Gly
            20                  25                  30          
Gln Tyr Ile Lys Ala Asn Ser Lys Phe Ile Gly Ile Thr
        35                  40                  45  


<210> 68
<211> 45
<212> PRT
<213> Artificial Sequence

<220> 
<223> CbpA-R21 sequence with incorporated cysteine
      residues and linked T cell epitope

<400> 68
Lys Cys Lys Ala Glu Asp Gln Lys Glu Glu Asp Arg Arg Asn Tyr Pro
 1               5                  10                  15      
Thr Asn Thr Tyr Lys Thr Leu Glu Leu Glu Cys Ala Gly Gly Pro Val
            20                  25                  30          
Val Glu Val Asn Gly Val Thr Ile Gln Val Gly Ser Arg
        35                  40                  45  


<210> 69
<211> 45
<212> PRT
<213> Artificial Sequence

<220> 
<223> CbpA-R21 sequence with incorporated cysteine
      residues and linked T cell epitope

<400> 69
Gln Tyr Ile Lys Ala Asn Ser Lys Phe Ile Gly Ile Thr Gln Tyr Ile
 1               5                  10                  15      
Lys Ala Asn Ser Lys Phe Ile Gly Ile Thr Gly Gly Gln Cys Glu Glu
            20                  25                  30          
Asp Arg Arg Asn Tyr Pro Thr Asn Thr Tyr Lys Cys Leu
        35                  40                  45  


<210> 70
<211> 45
<212> PRT
<213> Artificial Sequence

<220> 
<223> CbpA-R21 sequence with incorporated cysteine
      residues and linked T cell epitope

<400> 70
Pro Val Val Glu Val Asn Gly Val Thr Ile Gln Val Gly Ser Arg Gly
 1               5                  10                  15      
Gly Lys Cys Lys Ala Glu Asp Gln Lys Glu Glu Asp Arg Arg Asn Tyr
            20                  25                  30          
Pro Thr Asn Thr Tyr Lys Thr Leu Glu Leu Glu Cys Ala
        35                  40                  45  


<210> 71
<211> 45
<212> PRT
<213> Artificial Sequence

<220> 
<223> CbpA-R22 sequence with incorporated cysteine
      residues and linked T cell epitope

<400> 71
Lys Cys Glu Leu Glu Leu Val Lys Glu Glu Ala Lys Glu Pro Arg Asn
 1               5                  10                  15      
Glu Glu Lys Val Lys Gln Ala Lys Ala Glu Cys Glu Ser Lys Gly Gly
            20                  25                  30          
Gln Tyr Ile Lys Ala Asn Ser Lys Phe Ile Gly Ile Thr
        35                  40                  45  


<210> 72
<211> 45
<212> PRT
<213> Artificial Sequence

<220> 
<223> CbpA-R22 sequence with incorporated cysteine
      residues and linked T cell epitope

<400> 72
Lys Cys Glu Leu Glu Leu Val Lys Glu Glu Ala Lys Glu Pro Arg Asn
 1               5                  10                  15      
Glu Glu Lys Val Lys Gln Ala Lys Ala Glu Cys Glu Gly Gly Pro Val
            20                  25                  30          
Val Glu Val Asn Gly Val Thr Ile Gln Val Gly Ser Arg
        35                  40                  45  


<210> 73
<211> 45
<212> PRT
<213> Artificial Sequence

<220> 
<223> CbpA-R22 sequence with incorporated cysteine
      residues and linked T cell epitope

<400> 73
Gln Tyr Ile Lys Ala Asn Ser Lys Phe Ile Gly Ile Thr Gln Tyr Ile
 1               5                  10                  15      
Lys Ala Asn Ser Lys Phe Ile Gly Ile Thr Gly Gly Lys Glu Cys Ala
            20                  25                  30          
Lys Glu Pro Arg Asn Glu Glu Lys Val Lys Gln Cys Lys
        35                  40                  45  


<210> 74
<211> 45
<212> PRT
<213> Artificial Sequence

<220> 
<223> CbpA-R22 sequence with incorporated cysteine
      residues and linked T cell epitope

<400> 74
Pro Val Val Glu Val Asn Gly Val Thr Ile Gln Val Gly Ser Arg Gly
 1               5                  10                  15      
Gly Lys Cys Glu Leu Glu Leu Val Lys Glu Glu Ala Lys Glu Pro Arg
            20                  25                  30          
Asn Glu Glu Lys Val Lys Gln Ala Lys Ala Glu Cys Glu
        35                  40                  45  

1


1

LEGAL02/30532935v1

LEGAL02/30532935v1

