<110> KYOWA HAKKO KOGYO CO., LTD.

<120> Method of producing cytidine 5f-monophosphate

<130> 2006P00059

<160> 27

<170> PatentIn Ver. 2.1

<210> 1
<211> 217
<212> PRT
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Mutein

<400> 1
Val Ala Ala Pro Gln Arg Arg Lys Ser Met Lys Ile Val Glu Val Lys  16
                  5                  10                  15
His Pro Leu Val Lys His Lys Leu Gly Leu Met Arg Glu Gln Asp Ile  32
             20                  25                  30        
Ser Thr Lys Arg Phe Arg Glu Leu Ala Ser Glu Val Gly Ser Leu Leu  48
         35                  40                  45            
Thr Tyr Glu Ala Thr Ala Asp Leu Glu Thr Glu Lys Val Thr Ile Glu  64
     50                  55                  60                    
Gly Trp Asn Gly Pro Val Glu Ile Asp Gln Ile Lys Gly Lys Lys Ile  80
 65                  70                  75                  80
Trp Val Val Pro Ile Leu Arg Ala Gly Leu Gly Met Met Asp Gly Val  96
                 85                  90                  95    
Leu Gly Asn Val Pro Ser Ala Arg Ile Ser Val Val Gly Met Tyr Arg  112
            100                 105                 110        
Asn Glu Glu Thr Leu Glu Pro Val Pro Tyr Phe Gln Lys Leu Val Ser  128
        115                 120                 125              
Asn Ile Asp Glu Arg Met Ala Leu Ile Val Asp Pro Met Leu Ala Thr  144
    130                 135                 140                  
Gly Gly Ser Val Ile Ala Thr Ile Asp Leu Leu Lys Lys Ala Gly Cys  160
145                 150                 155                 160
Ser Ser Ile Lys Val Leu Val Leu Val Ala Ala Pro Glu Gly Ile Ala  176
                165                 170                 175    
Ala Leu Glu Lys Ala His Pro Asp Val Glu Lys Tyr Thr Ala Ser Ile  192
            180                 185                 190         
Asp Gln Gly Leu Asn Glu His Gly Lys Ile Ile Leu Gly Leu Gly Asp  208
        195                 200                 205             
Ala Gly Asp Lys Ile Phe Gly Tyr Lys                              217
    210                 215


<210> 2
<211> 217
<212> PRT
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Mutein

<400> 2
Val Ala Ala Pro Gln Arg Arg Lys Ser Met Lys Ile Val Glu Val Lys  16
                  5                  10                  15
His Pro Leu Val Lys His Lys Leu Gly Leu Met Arg Glu Gln Asp Ile  32
             20                  25                  30        
Ser Thr Lys Arg Phe Arg Glu Leu Ala Ser Glu Val Gly Ser Leu Leu  48
         35                  40                  45            
Thr Tyr Glu Ala Thr Ala Asp Leu Glu Thr Glu Lys Val Thr Ile Glu  64
     50                  55                  60                    
Gly Trp Asn Gly Pro Val Glu Ile Asp Gln Ile Lys Gly Lys Lys Ile  80
 65                  70                  75                  80
Trp Val Val Pro Ile Leu Arg Ala Gly Leu Gly Met Met Asp Gly Val  96
                 85                  90                  95    
Leu Gly Asn Val Pro Ser Ala Arg Ile Ser Val Val Gly Met Tyr Arg  112
            100                 105                 110        
Asn Glu Glu Thr Leu Glu Pro Val Pro Tyr Phe Gln Lys Leu Val Ser  128
        115                 120                 125              
Asn Ile Asp Glu Arg Met Ala Leu Ile Val Asp Pro Met Leu Ala Thr  144
    130                 135                 140                  
Gly Gly Ser Val Ile Ala Thr Ile Asp Leu Leu Lys Lys Ala Gly Cys  160
145                 150                 155                 160
Ser Ser Ile Lys Val Leu Val Leu Val Ala Ala Pro Glu Gly Ile Ala  176
                165                 170                 175    
Ala Leu Glu Lys Ala His Pro Asp Val Glu Lys Tyr Thr Ala Ser Ile  192
            180                 185                 190         
Asp Gln Gly Leu Asn Glu His Gly Tyr Ile Ile Pro Asp Leu Gly Asp  208
        195                 200                 205             
Ala Gly Asp Lys Ile Phe Gly Tyr Lys                              217
    210                 215


<210> 3
<211> 651
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Mutated DNA

<400> 3
GTG GCT GCC CCT CAA AGG AGA AAG AGT ATG AAG ATC GTG GAA GTC AAA@48
Val Ala Ala Pro Gln Arg Arg Lys Ser Met Lys Ile Val Glu Val Lys  16
                  5                  10                  15

CAC CCA CTC GTC AAA CAC AAG CTG GGA CTG ATG CGT GAG CAA GAT ATC  96
His Pro Leu Val Lys His Lys Leu Gly Leu Met Arg Glu Gln Asp Ile  32
             20                  25                  30        

AGC ACC AAG CGC TTT CGC GAA CTC GCT TCC GAA GTG GGT AGC CTG CTG  144
Ser Thr Lys Arg Phe Arg Glu Leu Ala Ser Glu Val Gly Ser Leu Leu  48
         35                  40                  45            

ACT TAC GAA GCG ACC GCC GAC CTC GAA ACG GAA AAA GTA ACT ATC GAA  192
Thr Tyr Glu Ala Thr Ala Asp Leu Glu Thr Glu Lys Val Thr Ile Glu  64
     50                  55                  60                    

GGC TGG AAC GGC CCG GTA GAA ATC GAC CAG ATC AAA GGT AAG AAA ATT  240
Gly Trp Asn Gly Pro Val Glu Ile Asp Gln Ile Lys Gly Lys Lys Ile  80
 65                  70                  75                  80

ACC GTT GTG CCA ATT CTG CGT GCG GGT CTT GGT ATG ATG GAC GGT GTG  288
Trp Val Val Pro Ile Leu Arg Ala Gly Leu Gly Met Met Asp Gly Val  96
                 85                  90                  95    

CTG GAA AAC GTT CCG AGC GCG CGC ATC AGC GTT GTC GGT ATG TAC CGT  336
Leu Gly Asn Val Pro Ser Ala Arg Ile Ser Val Val Gly Met Tyr Arg  112
            100                 105                 110        

AAT GAA GAA ACG CTG GAG CCG GTA CCG TAC TTC CAG AAA CTG GTT TCT  384
Asn Glu Glu Thr Leu Glu Pro Val Pro Tyr Phe Gln Lys Leu Val Ser  128
        115                 120                 125              

AAC ATC GAT GAG CGT ATG GCG CTG ATC GTT GAC CCA ATG CTG GCA ACC  432
Asn Ile Asp Glu Arg Met Ala Leu Ile Val Asp Pro Met Leu Ala Thr  144
    130                 135                 140                  

GGT GGT TCC GTT ATC GCG ACC ATC GAC CTG CTG AAA AAA GCG GGC TGC  480
Gly Gly Ser Val Ile Ala Thr Ile Asp Leu Leu Lys Lys Ala Gly Cys  160
145                 150                 155                 160

AGC AGC ATC AAA GTT CTG GTG CTG GTA GCT GCG CCA GAA GGT ATC GCT  528
Ser Ser Ile Lys Val Leu Val Leu Val Ala Ala Pro Glu Gly Ile Ala  176
                165                 170                 175    

GCG CTG GAA AAA GCG CAC CCG GAC GTC GAA CTG TAT ACC GCA TCG ATT  576
Ala Leu Glu Lys Ala His Pro Asp Val Glu Lys Tyr Thr Ala Ser Ile  192
            180                 185                 190         

GAT CAG GGA CTG AAC GAG CAC GGA AAG ATT ATT CTG GGC CTC GGC GAT  624
Asp Gln Gly Leu Asn Glu His Gly Lys Ile Ile Leu Gly Leu Gly Asp  208
        195                 200                 205             

GCC GGT GAC AAA ATC TTT GGT ACG AAA                              651
Ala Gly Asp Lys Ile Phe Gly Tyr Lys                              217
    210                 215


<210> 4
<211> 651
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Mutated DNA

<400> 4
GTG GCT GCC CCT CAA AGG AGA AAG AGT ATG AAG ATC GTG GAA GTC AAA@48
Val Ala Ala Pro Gln Arg Arg Lys Ser Met Lys Ile Val Glu Val Lys  16
                  5                  10                  15

CAC CCA CTC GTC AAA CAC AAG CTG GGA CTG ATG CGT GAG CAA GAT ATC  96
His Pro Leu Val Lys His Lys Leu Gly Leu Met Arg Glu Gln Asp Ile  32
             20                  25                  30        

AGC ACC AAG CGC TTT CGC GAA CTC GCT TCC GAA GTG GGT AGC CTG CTG  144
Ser Thr Lys Arg Phe Arg Glu Leu Ala Ser Glu Val Gly Ser Leu Leu  48
         35                  40                  45            

ACT TAC GAA GCG ACC GCC GAC CTC GAA ACG GAA AAA GTA ACT ATC GAA  192
Thr Tyr Glu Ala Thr Ala Asp Leu Glu Thr Glu Lys Val Thr Ile Glu  64
     50                  55                  60                    

GGC TGG AAC GGC CCG GTA GAA ATC GAC CAG ATC AAA GGT AAG AAA ATT  240
Gly Trp Asn Gly Pro Val Glu Ile Asp Gln Ile Lys Gly Lys Lys Ile  80
 65                  70                  75                  80

ACC GTT GTG CCA ATT CTG CGT GCG GGT CTT GGT ATG ATG GAC GGT GTG  288
Trp Val Val Pro Ile Leu Arg Ala Gly Leu Gly Met Met Asp Gly Val  96
                 85                  90                  95    

CTG GAA AAC GTT CCG AGC GCG CGC ATC AGC GTT GTC GGT ATG TAC CGT  336
Leu Gly Asn Val Pro Ser Ala Arg Ile Ser Val Val Gly Met Tyr Arg  112
            100                 105                 110        

AAT GAA GAA ACG CTG GAG CCG GTA CCG TAC TTC CAG AAA CTG GTT TCT  384
Asn Glu Glu Thr Leu Glu Pro Val Pro Tyr Phe Gln Lys Leu Val Ser  128
        115                 120                 125              

AAC ATC GAT GAG CGT ATG GCG CTG ATC GTT GAC CCA ATG CTG GCA ACC  432
Asn Ile Asp Glu Arg Met Ala Leu Ile Val Asp Pro Met Leu Ala Thr  144
    130                 135                 140                  

GGT GGT TCC GTT ATC GCG ACC ATC GAC CTG CTG AAA AAA GCG GGC TGC  480
Gly Gly Ser Val Ile Ala Thr Ile Asp Leu Leu Lys Lys Ala Gly Cys  160
145                 150                 155                 160

AGC AGC ATC AAA GTT CTG GTG CTG GTA GCT GCG CCA GAA GGT ATC GCT  528
Ser Ser Ile Lys Val Leu Val Leu Val Ala Ala Pro Glu Gly Ile Ala  176
                165                 170                 175    

GCG CTG GAA AAA GCG CAC CCG GAC GTC GAA CTG TAT ACC GCA TCG ATT  576
Ala Leu Glu Lys Ala His Pro Asp Val Glu Lys Tyr Thr Ala Ser Ile  192
            180                 185                 190         

GAT CAG GGA CTG AAC GAG CAC GGA TAC ATT ATT CCG GAC CTC GGC GAT  624
Asp Gln Gly Leu Asn Glu His Gly Tyr Ile Ile Pro Asp Leu Gly Asp  208
        195                 200                 205             

GCC GGT GAC AAA ATC TTT GGT ACG AAA                              651
Ala Gly Asp Lys Ile Phe Gly Tyr Lys                              217
    210                 215


<210> 5
<211> 651
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Mutated DNA

<400> 5
ATG GCT GCT CCA CAG CGC AGA AAG AGT ATG AAG ATC GTG GAA GTC AAA@48
Met Ala Ala Pro Gln Arg Arg Lys Ser Met Lys Ile Val Glu Val Lys  16
                  5                  10                  15

CAC CCA CTC GTC AAA CAC AAG CTG GGA CTG ATG CGT GAG CAA GAT ATC  96
His Pro Leu Val Lys His Lys Leu Gly Leu Met Arg Glu Gln Asp Ile  32
             20                  25                  30        

AGC ACC AAG TGC TTT CGC GAA CTC GCT TCC GAA GTG GGT AGC CTG CTG  144
Ser Thr Lys Cys Phe Arg Glu Leu Ala Ser Glu Val Gly Ser Leu Leu  48
         35                  40                  45            

ACT TAC GAA GCG ACC GCC GAC CTC GAA ACG GAA AAA GTA ACT ATC GAA  192
Thr Tyr Glu Ala Thr Ala Asp Leu Glu Thr Glu Lys Val Thr Ile Glu  64
     50                  55                  60                    

GGC TGG AAC GGC CCG GTA GAA ATC GAC CAG ATC AAA GGT AAG AAA ATT  240
Gly Trp Asn Gly Pro Val Glu Ile Asp Gln Ile Lys Gly Lys Lys Ile  80
 65                  70                  75                  80

ACC GTT GTG CCA ATT CTG CGT GCG GGT CTT GGT ATG ATG GAC GGT GTG  288
Trp Val Val Pro Ile Leu Arg Ala Gly Leu Gly Met Met Asp Gly Val  96
                 85                  90                  95    

CTG GAA AAC GTT CCG AGC GCG CGC ATC AGC GTT GTC GGT ATG TAC CGT  336
Leu Gly Asn Val Pro Ser Ala Arg Ile Ser Val Val Gly Met Tyr Arg  112
            100                 105                 110        

AAT GAA GAA ACG CTG GAG CCG GTA CCG TAC TTC CAG AAA CTG GTT TCT  384
Asn Glu Glu Thr Leu Glu Pro Val Pro Tyr Phe Gln Lys Leu Val Ser  128
        115                 120                 125              

AAC ATC GAT GAG CGT ATG GCG CTG ATC GTT GAC CCA TTG CTG GTA ACC  432
Asn Ile Asp Glu Arg Met Ala Leu Ile Val Asp Pro Leu Leu Val Thr  144
    130                 135                 140                  

GGT GGT TCC GTT ATC GCG ACC ATC GAC CTG CTG AAA AAA GCG GGC TGC  480
Gly Gly Ser Val Ile Ala Thr Ile Asp Leu Leu Lys Lys Ala Gly Cys  160
145                 150                 155                 160

AGC AGC ATC AAA GTT CTG GTG CTG GTA GCT GCG CCA GAA GGT ATC GCT  528
Ser Ser Ile Lys Val Leu Val Leu Val Ala Ala Pro Glu Gly Ile Ala  176
                165                 170                 175    

GCG CTG GAA AAA GCG CAC CCG GAC GTC GAA CTG TAT ACC GCA TCG ATT  576
Ala Leu Glu Lys Ala His Pro Asp Val Glu Lys Tyr Thr Ala Ser Ile  192
            180                 185                 190         

GAT CAG GGA CTG AAC GGG CAC GGA TAC ATT ATT CCG GAC CTC GGC GAT  624
Asp Gln Gly Leu Asn Gly His Gly Tyr Ile Ile Pro Asp Leu Gly Asp  208
        195                 200                 205             

GCC GGT GAC AAA ATC TTT GGT ACG AAA                              651
Ala Gly Asp Lys Ile Phe Gly Tyr Lys                              217
    210                 215


<210> 6
<211> 3105
<212> DNA
<213> Escherichia coli W3110 

<400> 6
tcggtgtggc gatgtttgcc attccggtgg gtaaggcaac cgggctggat attaatttgc 60

tgattgccgt ttccggttta ctgatgaccg tcaccgtctt ttttggcatt tcggcgctga 120

cggttctttc ggtgattgcg gttccggcta tcgcctgcct gggcggttat tccgtgtggc 180

tggctgttaa cggcatgggc ggcctggacg cattaaaagc ggtcgttccc gcacaaccgt 240

tagatttcaa tgtcgcgctg gcgctggttg tggggtcatt tatcagtgcg ggtacgctca 300

ccgctgactt tgtccggttt ggtcgcaatg ccaaactggc ggtgctggtg gcgatggtgg 360

cctttttcct cggcaactcg ttgatgttta ttttcggtgc agcgggcgct gcggcactgg 420

gcatggcgga tatctctgat gtgatgattg ctcagggcct gctgctgcct gcgattgtgg 480

tgctggggct gaatatctgg accaccaacg ataacgcact ctatgcgtcg ggtttaggtt 540

tcgccaacat taccgggatg tcgagcaaaa ccctttcggt aatcaacggt attatcggta 600

cggtctgcgc attatggctg tataacaatt ttgtcggctg gttgaccttc ctttcggcag 660

ctattcctcc agtgggtggc gtgatcatcg ccgactatct gatgaaccgt cgccgctatg 720

agcactttgc gaccacgcgt atgatgagtg tcaattgggt ggcgattctg gcggtcgcct 780

tggggattgc tgcaggccac tggttaccgg gaattgttcc ggtcaacgcg gtattaggtg 840

gcgcgctgag ctatctgatc cttaacccga ttttgaatcg taaaacgaca gcagcaatga 900

cgcatgtgga ggctaacagt gtcgaataac gctttacaaa caattattaa cgcccggtta 960

ccaggcgaag aggggctgtg gcagattcat ctgcaggacg gaaaaatcag cgccattgat 1020

gcgcaatccg gcgtgatgcc cataactgaa aacagcctgg atgccgaaca aggtttagtt 1080

ataccgccgt ttgtggagcc acatattcac ctggacacca cgcaaaccgc cggacaaccg 1140

aactggaatc agtccggcac gctgtttgaa ggcattgaac gctgggccga gcgcaaagcg 1200

ttattaaccc atgacgatgt gaaacaacgc gcatggcaaa cgctgaaatg gcagattgcc 1260

aacggcattc agcatgtgcg tacccatgtc gatgtttcgg atgcaacgct aactgcgctg 1320

aaagcaatgc tggaagtgaa gcaggaagtc gcgccgtgga ttgatctgca aatcgtcgcc 1380

ttccctcagg aagggatttt gtcgtatccc aacggtgaag cgttgctgga agaggcgtta 1440

cgcttagggg cagatgtagt gggggcgatt ccgcattttg aatttacccg tgaatacggc 1500

gtggagtcgc tgcataaaac cttcgccctg gcgcaaaaat acgaccgtct catcgacgtt 1560

cactgtgatg agatcgatga cgagcagtcg cgctttgtcg aaaccgttgc tgccctggcg 1620

caccatgaag gcatgggcgc gcgagtcacc gccagccaca ccacggcaat gcactcctat 1680

aacggggcgt atacctcacg cctgttccgc ttgctgaaaa tgtccggtat taactttgtc 1740

gccaacccgc tggtcaatat tcatctgcaa ggacgtttcg atacgtatcc aaaacgtcgc 1800

ggcatcacgc gcgttaaaga gatgctggag tccggcatta acgtctgctt tggtcacgat 1860

gatgtcttcg atccgtggta tccgctggga acggcgaata tgctgcaagt gctgcatatg 1920

gggctgcatg tttgccagtt gatgggctac gggcagatta acgatggcct gaatttaatc 1980

acccaccaca gcgcaaggac gttgaatttg caggattacg gcattgccgc cggaaacagc 2040

gccaacctga ttatcctgcc ggctgaaaat gggtttgatg cgctgcgccg tcaggttccg 2100

gtacgttatt cggtacgtgg cggcaaggtg attgccagca cacaaccggc acaaaccacc 2160

gtatatctgg agcagccaga agccatcgat tacaaacgtt gaacgactgg gttacagcga 2220

gcttagttta tgccggatgc ggcgtgaacg ccttatccgg cctacgtaga gcactgaact 2280

cgtaggcctg ataagcgtag cgcatcaggc aattccagcc gctgatctgt gtcagcggct 2340

accgtgattc attcccgcca acaaccgcgc attcctccaa cgccatgtgc aaaaatgcct 2400

tcgcagcggc tgtctgccag ctgtagttta tgccggatgc ggcgtgaacg ccttatccgg 2460

cctacgtaga gcactgaact cgtaggcctg ataagcgtag cgcatcaggc aattccagcc 2520

gcagacctgt gtcagcggct accgtgattc atttccgcca acaaccgcgc atttatccaa 2580

cgccatgtgc aaaaatgcct tcgcggcggc tgtctgccag ctatttttcc gccgcaacaa 2640

aaccgccgtt ctctccagta gtggcggggc aagagaaata gctttaagcc cgtcatgttg 2700

tgtggcaatc gctgctggta acaatgtgga aagggaagtg cggcgaatca gctccagaac 2760

cgcgctaatt gagttcgcct caatgaccac ctgtggatgt agccccgctt tctcgcagta 2820

gtggtcaatt tgctctctgg tggcaaattc cgcgctgagc aggaccagtt tttcatcatg 2880

caagcgactc aacgccacct gttcatggac ggccagcgga tgatgttgcg ccacgactaa 2940

cgctaaactt tctgtcagta aaggaattgc ctccagctcc ggcgaatgca caggcgcgaa 3000

ggcaatccca acgtccaact cgtcgcggca aagcatatcc tcgattttct cctgcgacat 3060

ttcctgtagc tggagcgtga tgctgggata gcgcgcatag aaatc                 3105


<210> 7
<211> 46
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Synthetic DNA

<400> 7
gccattgatgcgcaatccggggatccgtgtaggctggagctgcttc                    46

<210> 8
<211> 47
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Synthetic DNA

<400> 8
ccgaataacgtaccggaaccaagcttatgaatatcctccttagttcc                   47


<210> 9
<211> 2572
<212> DNA
<213> Escherichia coli W3110 

<400> 9
gagctggagt tcgcaactgg tacacggtga acgtaaagtc gtaggtcaga aaggatcaga 60

agaatgatgg cgaatatctg gtggtcatta ccgctgacat tgattgtctt ttttgccgcc 120

cgcaaactgg cggcacggta taagtttccc ttgcttaacc cgttgctggt agcaatggtg 180

gtgatcattc cttttttaat gctgactggc atctcttacg acagctactt taaaggtagc 240

gaagtgctaa acgatctgct gcaaccggcg gtcgtcgcgc tggcctatcc tttatatgag 300

cagctacacc agatccgcgc gcgctggaaa tcgatcatca ccatctgttt tatcggcagc 360

gtggttgcga tggtgacggg gacttccgtg gcattgttga tgggcgcttc accggaaatc 420

gccgcgtcaa tcctgccgaa atcagtcacc acgcctattg caatggcggt tggcggcagt 480

attggcggta ttccggcaat cagcgcagtt tgcgtgattt tcgtcggcat cctcggcgcg 540

gtatttggcc atacattgct taatgcgatg cgtattcgta ccaaagctgc gcgcggtctg 600

gcaatgggga ctgcctcgca cgccctcggt acggcgcgct gcgccgagct ggattatcag 660

gaaggtgcat ttagttcgct agcgctggtg ttatgcggga taattacttc gctgatcgca 720

ccgttccttt tcccgattat tctggcagta atgggctaaa atttgcgatg cgtcgcgcat 780

ttttgatgta tgtttcacgc gttgcataat taatgagatt cagatcacat ataaagccac 840

aacgggttcg taaactgtta tcccattaca tgattatgag gcaacgccat gcatccacgt 900

tttcaaaccg cttttgccca acttgcggat aacttgcaat ctgcactgga acctattctg 960

gcagacaagt acttccccgc tttgttgacc ggggagcaag tctcatcgct gaagagcgca 1020

acggggctgg acgaagacgc gctggcattc gcactacttc cgctggcggc ggcctgtgcg 1080

cgtacgccat tgtcgaattt taatgttggc gcaattgcgc gcggtgtgag cggaacctgg 1140

tatttcggtg ccaatatgga atttattggt gcgacaatgc agcaaaccgt tcatgccgaa 1200

caaagcgcga tcagccacgc ctggttgagt ggtgaaaaag cgcttgcagc catcaccgtt 1260

aactacacgc cttgtggtca ctgccgtcag tttatgaatg aactgaacag cggtctggat 1320

ctgcgtattc atctgccggg ccgcgaggca cacgcgctgc gtgactatct gccagatgcc 1380

tttgggccga aagatctgga gattaaaacg ctgctgatgg acgaacagga tcacggctat 1440

gcgctgacgg gtgatgcgct ttctcaggca gcgattgcgg cggcaaaccg ttcgcacatg 1500

ccttacagta agtcgccaag cggtgtcgcg ctggaatgta aagacggtcg tattttcagt 1560

ggcagctacg ctgaaaacgc cgcattcaac ccgactctgc caccgttgca gggagcgtta 1620

attctgttga atctcaaggg ttatgattac ccggatatcc agcgcgcggt tctggcagaa 1680

aaagccgatg cgccgttgat tcagtgggat gccacctccg caacgctgaa agctctcggc 1740

tgtcacagta tcgaccgagt gcttctcgct taagcctggt gccggatgcg gcgtgaacgc 1800

cttgtccggc ttgccagccc tctcctggtg tcgaaattcc cggcaaacag tttgccgttt 1860

cttgcgcaaa accagcgggt aaagtagcct gatggaaatt ttccttagat cgagtctcct 1920

gcatgttaaa gcgcgtgttc ctcagcctgt tagtcctgat cggcttgctg ctgttgactg 1980

tgctcggcct cgatcgctgg atgagctgga aaaccgcgcc ttatatctac gacgaattgc 2040

aggatctccc ctaccgccag gtcggtgtgg tgctcggaac agcaaaatat tatcgtactg 2100

gcgtaattaa tcagtattat cgctaccgca ttcaaggagc gattaatgcc tataacagcg 2160

gtaaggtaaa ttatctatta ctgagcggcg ataacgcatt gcaaagttat aatgagccga 2220

tgaccatgcg caaagattta atcgctgctg gtgtcgaccc atcagatatt gttctcgatt 2280

acgcaggctt tcgtacgctg gattccatcg tgcgtacacg caaagttttc gatactaatg 2340

atttcattat tatcacccaa cgtttccact gtgagcgagc attatttatt gcgctgcata 2400

tggggattca ggctcagtgt tatgccgtac cgtcaccgaa agatatgctg tcagtacgta 2460

ttcgtgaatt tgccgcccgt ttcggtgcgc tggctgacct ttatattttt aaacgtgaac 2520

cgcgtttttt agggccgctg gtccctattc cggctatgca ccaggtaccg ga         2572


<210> 10
<211> 44
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Synthetic DNA

<400> 10
ggagcaagtc tcatcgctgg atccgtgtag gctggagctg cttc                  44
44                

<210> 11
<211> 47
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Synthetic DNA

<400> 11
ccagaaccgc gcgctggata aagcttatga atatcctcct tagttcc               47


<210> 12
<211> 1192
<212> DNA
<213> Escherichia coli MM294 

<400> 12
ggatccgtac atcaacgtcg atccaggtac tatgagccca atccaacacg gggaagtgtt 60

cgttactgaa gacggcgctg aaaccgacct ggacctgggg cactacgagc gtttcattcg 120

taccaaaatg agccgccgca acaacttcac cacgggtcgt atctactctg acgttctgcg 180

taaagaacgc cgcggtgact acctcggcgc aaccgtgcag gttattccgc acatcactaa 240

cgcaatcaaa gagcgcgtgc tggaaggtgg cgaaggtcat gacgtagtac tggtagaaat 300

cggcggtaca gtaggtgata tcgaatcctt gccgttcctc gaagcgattc gccagatggc 360

tgttgaaatt ggccgtgagc acactctgtt tatgcacctg acgctggtgc cgtacatggc 420

agcgtctggt gaagtcaaaa ccaaaccgac tcagcactct gtaaaagagc tgctctccat 480

cggtatccag cctgacatcc tgatttgtcg ttcagatcgc gctgttccgg cgaacgaacg 540

tgcgaagatt gcattgttct gtaatgttcc ggaaaaagcg gttatttctc tgaaagacgt 600

cgattccatc tataaaattc cgggcctgtt gaaatctcag gggctggacg attatatttg 660

taaacgattc agcttaaact gcccggaagc gaatctgtcc gaatgggaac aggttatctt 720

cgaagaagcg aacccggtaa gtgaagtcac catcggtatg gtcggcaagt acattgaact 780

gccggatgct tataaatcag tgatcgaagc actgaaacac ggtgggctga agaatcgtgt 840

cagcgtcaac atcaaactga tcgattcaca agatgttgaa acgcgcggcg ttgaaatcct 900

taaaggtctg gacgcaatcc tcgtacctgg cggtttcggc tatcgtggcg tagaaggcat 960

gattacgacc gcgcgttttg cgcgtgagaa caatattcct tatctgggca tttgcctggg 1020

tatgcaggtg gcgttaattg attacgctcg ccatgttgcc aacatggaga acgccaactc 1080

tacggaattt gtgccagact gtaagtaccc ggttgtggcg ctgattaccg agtggcgcga 1140

tgaaaacggc aacgttgaag ttcgtagcga gaagagcgat ctcggcggta cc         1192


<210> 13
<211> 34
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Synthetic DNA

<400> 13
cggaattctt gtggctgccc ctcaaaggag aaag                             34


<210> 14
<211> 41
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Synthetic DNA

<400> 14
cgggatcctc tttatttcgt accaaagatt ttgtcaccgg c                     41


<210> 15
<211> 217
<212> PRT
<213> Escherichia coli W3110

<400> 15
Val Ala Ala Pro Gln Arg Arg Lys Ser Met Lys Ile Val Glu Val Lys  16
                  5                  10                  15
His Pro Leu Val Lys His Lys Leu Gly Leu Met Arg Glu Gln Asp Ile  32
             20                  25                  30        
Ser Thr Lys Arg Phe Arg Glu Leu Ala Ser Glu Val Gly Ser Leu Leu  48
         35                  40                  45            
Thr Tyr Glu Ala Thr Ala Asp Leu Glu Thr Glu Lys Val Thr Ile Glu  64
     50                  55                  60                    
Gly Trp Asn Gly Pro Val Glu Ile Asp Gln Ile Lys Gly Lys Lys Ile  80
 65                  70                  75                  80
Trp Val Val Pro Ile Leu Arg Ala Gly Leu Gly Met Met Asp Gly Val  96
                 85                  90                  95    
Leu Gly Asn Val Pro Ser Ala Arg Ile Ser Val Val Gly Met Tyr Arg  112
            100                 105                 110        
Asn Glu Glu Thr Leu Glu Pro Val Pro Tyr Phe Gln Lys Leu Val Ser  128
        115                 120                 125              
Asn Ile Asp Glu Arg Met Ala Leu Ile Val Asp Pro Met Leu Ala Thr  144
    130                 135                 140                  
Gly Gly Ser Val Ile Ala Thr Ile Asp Leu Leu Lys Lys Ala Gly Cys  160
145                 150                 155                 160
Ser Ser Ile Lys Val Leu Val Leu Val Ala Ala Pro Glu Gly Ile Ala  176
                165                 170                 175    
Ala Leu Glu Lys Ala His Pro Asp Val Glu Lys Tyr Thr Ala Ser Ile  192
            180                 185                 190         
Asp Gln Gly Leu Asn Glu His Gly Tyr Ile Ile Pro Gly Leu Gly Asp  208
        195                 200                 205             
Ala Gly Asp Lys Ile Phe Gly Tyr Lys                              217
    210                 215


<210> 16
<211> 651
<212> DNA
<213> Eschrichia coli W3110

<400> 16
GTG GCT GCC CCT CAA AGG AGA AAG AGT ATG AAG ATC GTG GAA GTC AAA@48
Val Ala Ala Pro Gln Arg Arg Lys Ser Met Lys Ile Val Glu Val Lys  16
                  5                  10                  15

CAC CCA CTC GTC AAA CAC AAG CTG GGA CTG ATG CGT GAG CAA GAT ATC  96
His Pro Leu Val Lys His Lys Leu Gly Leu Met Arg Glu Gln Asp Ile  32
             20                  25                  30        

AGC ACC AAG CGC TTT CGC GAA CTC GCT TCC GAA GTG GGT AGC CTG CTG  144
Ser Thr Lys Arg Phe Arg Glu Leu Ala Ser Glu Val Gly Ser Leu Leu  48
         35                  40                  45            

ACT TAC GAA GCG ACC GCC GAC CTC GAA ACG GAA AAA GTA ACT ATC GAA  192
Thr Tyr Glu Ala Thr Ala Asp Leu Glu Thr Glu Lys Val Thr Ile Glu  64
     50                  55                  60                    

GGC TGG AAC GGC CCG GTA GAA ATC GAC CAG ATC AAA GGT AAG AAA ATT  240
Gly Trp Asn Gly Pro Val Glu Ile Asp Gln Ile Lys Gly Lys Lys Ile  80
 65                  70                  75                  80

ACC GTT GTG CCA ATT CTG CGT GCG GGT CTT GGT ATG ATG GAC GGT GTG  288
Trp Val Val Pro Ile Leu Arg Ala Gly Leu Gly Met Met Asp Gly Val  96
                 85                  90                  95    

CTG GAA AAC GTT CCG AGC GCG CGC ATC AGC GTT GTC GGT ATG TAC CGT  336
Leu Gly Asn Val Pro Ser Ala Arg Ile Ser Val Val Gly Met Tyr Arg  112
            100                 105                 110        

AAT GAA GAA ACG CTG GAG CCG GTA CCG TAC TTC CAG AAA CTG GTT TCT  384
Asn Glu Glu Thr Leu Glu Pro Val Pro Tyr Phe Gln Lys Leu Val Ser  128
        115                 120                 125              

AAC ATC GAT GAG CGT ATG GCG CTG ATC GTT GAC CCA ATG CTG GCA ACC  432
Asn Ile Asp Glu Arg Met Ala Leu Ile Val Asp Pro Met Leu Ala Thr  144
    130                 135                 140                  

GGT GGT TCC GTT ATC GCG ACC ATC GAC CTG CTG AAA AAA GCG GGC TGC  480
Gly Gly Ser Val Ile Ala Thr Ile Asp Leu Leu Lys Lys Ala Gly Cys  160
145                 150                 155                 160

AGC AGC ATC AAA GTT CTG GTG CTG GTA GCT GCG CCA GAA GGT ATC GCT  528
Ser Ser Ile Lys Val Leu Val Leu Val Ala Ala Pro Glu Gly Ile Ala  176
                165                 170                 175    

GCG CTG GAA AAA GCG CAC CCG GAC GTC GAA CTG TAT ACC GCA TCG ATT  576
Ala Leu Glu Lys Ala His Pro Asp Val Glu Lys Tyr Thr Ala Ser Ile  192
            180                 185                 190         

GAT CAG GGA CTG AAC GAG CAC GGA TAC ATT ATT CCG GGC CTC GGC GAT  624
Asp Gln Gly Leu Asn Glu His Gly Tyr Ile Ile Pro Gly Leu Gly Asp  208
        195                 200                 205             

GCC GGT GAC AAA ATC TTT GGT ACG AAA                              651
Ala Gly Asp Lys Ile Phe Gly Tyr Lys                              217
    210                 215


<210> 17
<211> 33
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Synthetic DNA

<400> 17
ctgatcgttg acccannnct ggcaaccggt ggt                              33 


<210> 18
<211> 33
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Synthetic DNA

<400> 18
accaccggtt gccagnnntg ggtcaacgat cag                              33 


<210> 19
<211> 30
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Synthetic DNA

<400> 19
ctgaacgagc acggannnat tattccgggc                                  30 


<210> 20
<211> 30
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Synthetic DNA

<400> 20
gcccggaata atnnntccgt gctcgttcag                                  30 


<210> 21
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Synthetic DNA

<400> 21
cggctcgtat aatgtgtgga                                             20 


<210> 22
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Synthetic DNA

<400> 22
cgccaaaaca gccaagcttg c                                           21 


<210> 23
<211> 35
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Synthetic DNA

<400> 23
gggatccgca tgcttgtggc tgcccctcaa aggag                            35 


<210> 24
<211> 33
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Synthetic DNA

<400> 24
gttgacccat tgctggttac cggtggttcc gtt                              33 


<210> 25
<211> 33
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Synthetic DNA

<400> 25
aacggaacca ccggtaacca gcaatgggtc aac                              33 


<210> 26
<211> 30
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Synthetic DNA

<400> 26
gacgcgtcga ctatggctgc tccacagcgc                                  30


<210> 27
<211> 715
<212> DNA
<213> Corynebacterium

<400> 27
cctgcaggtc atcacccgag caggcgaccc gaacgttcgg aggctcctcg ctgtccattc 60

gctcccctgg cgcggtatga accgccgcct catagtgcag tttgatcctg acgagcccag 120

catgtctgcg cccaccttcg cggaacctga ccagggtccg ctagcgggcg gccggaaggt 180

gaatgctagg catgatctaa ccctcggtct ctggcgtcgc gactgcgaaa tttcgcgagg 240

gtttccgaga aggtgattgc gcttcgcaga tctcgtggac ggcttggttg acgccctccg 300

cccattgggt gatggtggca ccatttggct gttgactcct ggtgcaggaa aacgtggaac 360

tattgctcca ggtgaaattt ccgaatccgc acaattggca ggcctcgtcc agaccaccgc 420

agagcgtctc ggtgattggc agggcagctg cttggtcgcg cgcggcgcga tgaagaagta 480

agaattagcc gaaaacacct tccagccagg cgatttgctt aagttagaag gtgtggctag 540

tattctaaga gtgctcatga ggaagcggaa agcttttaag agagcatgat gcggctttag 600

ctcagctgga agagcaactg gtttacaccc agtaggtcgg gggttcgatc cagctgtgaa 660

caattgcact ttggatctaa ttaagggatt agtcgactat ggatccccgg gtacc      715

<210> 28
<211> 28
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Synthetic DNA

<400> 28
aataaagctt accgaattca aaaatcag                                     28

<210> 29
<211> 30
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Synthetic DNA

<400> 29
tcgcggatcc tcatttaaca atctccgcta                                   30


<210> 30
<211> 217
<212> PRT
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Mutein

<400> 30
Val Ala Ala Pro Gln Arg Arg Lys Ser Met Lys Ile Val Glu Val Lys  16
                  5                  10                  15
His Pro Leu Val Lys His Lys Leu Gly Leu Met Arg Glu Gln Asp Ile  32
             20                  25                  30        
Ser Thr Lys Arg Phe Arg Glu Leu Ala Ser Glu Val Gly Ser Leu Leu  48
         35                  40                  45            
Thr Tyr Glu Ala Thr Ala Asp Leu Glu Thr Glu Lys Val Thr Ile Glu  64
     50                  55                  60                    
Gly Trp Asn Gly Pro Val Glu Ile Asp Gln Ile Lys Gly Lys Lys Ile  80
 65                  70                  75                  80
Trp Val Val Pro Ile Leu Arg Ala Gly Leu Gly Met Met Asp Gly Val  96
                 85                  90                  95    
Leu Gly Asn Val Pro Ser Ala Arg Ile Ser Val Val Gly Met Tyr Arg  112
            100                 105                 110        
Asn Glu Glu Thr Leu Glu Pro Val Pro Tyr Phe Gln Lys Leu Val Ser  128
        115                 120                 125              
Asn Ile Asp Glu Arg Met Ala Leu Ile Val Asp Pro Met Leu Ala Thr  144
    130                 135                 140                  
Gly Gly Ser Val Ile Ala Thr Ile Asp Leu Leu Lys Lys Ala Gly Cys  160
145                 150                 155                 160
Ser Ser Ile Lys Val Leu Val Leu Val Ala Ala Pro Glu Gly Ile Ala  176
                165                 170                 175    
Ala Leu Glu Lys Ala His Pro Asp Val Glu Lys Tyr Thr Ala Ser Ile  192
            180                 185                 190         
Asp Gln Gly Leu Asn Glu His Gly Tyr Ile Ile Pro Glu Leu Gly Asp  208
        195                 200                 205             
Ala Gly Asp Lys Ile Phe Gly Tyr Lys                              217
    210                 215


<210> 31
<211> 244
<212> PRT
<213> Toxoplasma gondii RH uracil phosphoribosyl transferase 

<400> 31
Met Ala Gln Val Pro Ala Ser Gly Lys Leu Leu Val Asp Pro Arg Tyr  16
  1               5                  10                  15
Ser Thr Asn Asp Gln Glu Glu Ser Ile Leu Gln Asp Ile Ile Thr Arg  32
             20                  25                  30
Phe Pro Asn Val Val Leu Met Lys Gln Thr Ala Gln Leu Arg Ala Met  48
         35                  40                  45
Met Thr Ile Ile Arg Asp Lys Glu Thr Pro Lys Glu Glu Phe Val Phe  64
     50                  55                  60
Tyr Ala Asp Arg Leu Ile Arg Leu Leu Ile Glu Glu Ala Leu Asn Glu  80
 65                  70                  75                  80
Leu Pro Phe Glu Lys Lys Glu Val Thr Thr Pro Leu Asp Val Ser Tyr  96
                 85                  90                  95
His Gly Val Ser Phe Tyr Ser Lys Ile Cys Gly Val Ser Ile Val Arg  112
            100                 105                 110
Ala Gly Glu Ser Met Glu Ser Gly Leu Arg Ala Val Cys Arg Gly Cys  128
        115                 120                 125
Arg Ile Gly Lys Ile Leu Ile Gln Arg Asp Glu Thr Thr Ala Glu Pro  144
    130                 135                 140
Lys Leu Ile Tyr Glu Lys Leu Pro Ala Asp Ile Arg Asp Arg Trp Val  160
145                 150                 155                 160
Met Leu Leu Asp Pro Met Cys Ala Thr Ala Gly Ser Val Cys Lys Ala  176
                165                 170                 175
Ile Glu Val Leu Leu Arg Leu Gly Val Lys Glu Glu Arg Ile Ile Phe  192
            180                 185                 190
Val Asn Ile Leu Ala Ala Pro Gln Gly Ile Glu Arg Val Phe Lys Glu  208
        195                 200                 205
Tyr Pro Lys Val Arg Met Val Thr Ala Ala Val Asp Ile Cys Leu Asn  224
    210                 215                 220
Ser Arg Tyr Tyr Ile Val Pro Gly Ile Gly Asp Phe Gly Asp Arg Tyr  240
225                 230                 235                 240
Phe Gly Thr Met                                                  244



<210> 32
<211> 209
<212> PRT
<213> Bacillus caldolyticus uracil phosphoribosyltransferase

<400> 32
Met Gly Lys Val Tyr Val Phe Asp His Pro Leu Ile Gln His Lys Leu  16
  1               5                  10                  15
Thr Tyr Ile Arg Asp Lys Asn Thr Gly Thr Lys Glu Phe Arg Glu Leu  32
             20                  25                  30
Val Asp Glu Val Ala Thr Leu Met Ala Phe Glu Ile Thr Arg Asp Leu  48
         35                  40                  45
Pro Leu Glu Glu Val Glu Ile Glu Thr Pro Val Ser Lys Ala Arg Ala  64
     50                  55                  60
Lys Val Ile Ala Gly Lys Lys Leu Gly Val Ile Pro Ile Leu Arg Ala  80
 65                  70                  75                  80
Gly Ile Gly Met Val Asp Gly Ile Leu Lys Leu Ile Pro Ala Ala Lys  96
                 85                  90                  95
Val Gly His Ile Gly Leu Tyr Arg Asp Pro Gln Thr Leu Lys Pro Val  112
            100                 105                 110
Glu Tyr Tyr Val Lys Leu Pro Ser Asp Val Glu Glu Arg Asp Phe Ile  128
        115                 120                 125
Ile Val Asp Pro Met Leu Ala Thr Gly Gly Ser Ala Val Ala Ala Ile  144
    130                 135                 140
Asp Ala Leu Lys Lys Arg Gly Ala Lys Ser Ile Lys Phe Met Cys Leu  160
145                 150                 155                 160
Ile Ala Ala Pro Glu Gly Val Lys Ala Val Glu Thr Ala His Pro Asp  176
                165                 170                 175
Val Asp Ile Tyr Ile Ala Ala Leu Asp Glu Arg Leu Asn Asp His Gly  192
            180                 185                 190
Tyr Ile Val Pro Gly Leu Gly Asp Ala Gly Asp Arg Leu Phe Gly Thr  208
        195                 200                 205
Lys                                                              209



<210> 33
<211> 244
<212> PRT
<213> Escherichia coli uracil phosphoribosyltransferase

<400> 33
Met Lys Ile Val Glu Val Lys His Pro Leu Val Lys His Lys Leu Gly  16
  1               5                  10                  15
Leu Met Arg Glu Gln Asp Ile Ser Thr Lys Arg Phe Arg Glu Leu Ala  32
             20                  25                  30
Ser Glu Val Gly Ser Leu Leu Thr Tyr Glu Ala Thr Ala Asp Leu Glu  48
         35                  40                  45
Thr Glu Lys Val Thr Ile Glu Gly Trp Asn Gly Pro Val Glu Ile Asp  64
     50                  55                  60
Gln Ile Lys Gly Lys Lys Ile Thr Val Val Pro Ile Leu Arg Ala Gly  80
 65                  70                  75                  80
Leu Gly Met Met Asp Gly Val Leu Glu Asn Val Pro Ser Ala Arg Ile  96
                 85                  90                  95
Ser Val Val Gly Met Tyr Arg Asn Glu Glu Thr Leu Glu Pro Val Pro  112
            100                 105                 110
Tyr Phe Gln Lys Leu Val Ser Asn Ile Asp Glu Arg Met Ala Leu Ile  128
        115                 120                 125
Val Asp Pro Met Leu Ala Thr Gly Gly Ser Val Ile Ala Thr Ile Asp  144
    130                 135                 140
Leu Leu Lys Lys Ala Gly Cys Ser Ser Ile Lys Val Leu Val Leu Val  160
145                 150                 155                 160
Ala Ala Pro Glu Gly Ile Ala Ala Leu Glu Lys Ala His Pro Asp Val  176
                165                 170                 175
Glu Leu Tyr Thr Ala Ser Ile Asp Gln Gly Leu Asn Glu His Gly Tyr  192
            180                 185                 190
Ile Ile Pro Gly Leu Gly Asp Ala Gly Asp Lys Ile Phe Gly Thr Lys  208
        195                 200                 205


<210> 34
<211> 39
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Synthetic DNA

<400> 34
accatgggat ccgcgcgaag atcgtggaag tcaaacacc                          39


<210> 35
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Synthetic DNA

<400> 35
ttgatgctgc tcgagcccgc ttt                                           23


<210> 36
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Synthetic DNA

<400> 36
aaaaagcggg ctcgagcagc atc                                           23


<210> 37
<211> 37
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Synthetic DNA

<400> 37
gcatgcggat cctctttatt tcgtaccaaa gattttg                            37


<210> 38
<211> 221
<212> PRT
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: Mutein

<400> 38
 Met Arg Gly Ser His His His His His His Gly Ile Arg Ala Lys Ile  16
   1               5                  10                  15    
 Val Glu Val Lys His Pro Leu Val Lys His Lys Leu Gly Leu Met Arg  32
              20                  25                  30        
 Glu Gln Asp Ile Ser Thr Lys Arg Phe Arg Glu Leu Ala Ser Glu Val  48
          35                  40                  45            
 Gly Ser Leu Leu Thr Tyr Glu Ala Thr Ala Asp Leu Glu Thr Glu Lys  64
      50                  55                  60                
 Val Thr Ile Glu Gly Trp Asn Gly Pro Val Glu Ile Asp Gln Ile Lys  80
  65                  70                  75                  80
 Gly Lys Lys Ile Thr Val Val Pro Ile Leu Arg Ala Gly Leu Gly Met  96
                  85                  90                  95    
 Met Asp Gly Val Leu Glu Asn Val Pro Ser Ala Arg Ile Ser Val Val  112
             100                 105                 110        
 Gly Met Tyr Arg Asn Glu Glu Thr Leu Glu Pro Val Pro Tyr Phe Gln  128
         115                 120                 125            
 Lys Leu Val Ser Asn Ile Asp Glu Arg Met Ala Leu Ile Val Asp Pro  144
     130                 135                 140                
 Met Leu Ala Thr Gly Gly Ser Val Ile Ala Thr Ile Asp Leu Leu Lys  160
 145                 150                 155                 160
 Lys Ala Gly Ser Ser Ser Ile Lys Val Leu Val Leu Val Ala Ala Pro  176
                 165                 170                 175    
 Glu Gly Ile Ala Ala Leu Glu Lys Ala His Pro Asp Val Glu Leu Tyr  192
             180                 185                 190        
 Thr Ala Ser Ile Asp Gln Gly Leu Asn Glu His Gly Tyr Ile Ile Pro  208
         195                 200                 205            
 Gly Leu Gly Asp Ala Gly Asp Lys Ile Phe Gly Thr Lys              221
     210                 215                 220    
       

