                         SEQUENCE LISTING

<110>  Institut Gustave Roussy and Institut National de la Sant Et de la Recherche Mdicale
 
<120>  Inhibitors of protein phosphatase 1, GADD34 and protein 
       phosphatase 1/GADD34 complex, preparation and uses thereof.

<130>  B0556WO

<160>  15    

<170>  PatentIn version 3.3

<210>  1
<211>  674
<212>  PRT
<213>  Artificial

<220>
<223>  Human GADD34

<400>  1

Met Ala Pro Gly Gln Ala Pro His Gln Ala Thr Pro Trp Arg Asp Ala 
1               5                   10                  15      


His Pro Phe Phe Leu Leu Ser Pro Val Met Gly Leu Leu Ser Arg Ala 
            20                  25                  30          


Trp Ser Arg Leu Arg Gly Leu Gly Pro Leu Glu Pro Trp Leu Val Glu 
        35                  40                  45              


Ala Val Lys Gly Ala Ala Leu Val Glu Ala Gly Leu Glu Gly Glu Ala 
    50                  55                  60                  


Arg Thr Pro Leu Ala Ile Pro His Thr Pro Trp Gly Arg Arg Pro Gly 
65                  70                  75                  80  


Glu Glu Ala Glu Asp Ser Gly Gly Pro Gly Glu Asp Arg Glu Thr Leu 
                85                  90                  95      


Gly Leu Lys Thr Ser Ser Ser Leu Pro Glu Ala Trp Gly Leu Leu Asp 
            100                 105                 110         


Asp Asp Asp Gly Met Tyr Gly Glu Arg Glu Ala Thr Ser Val Pro Arg 
        115                 120                 125             


Gly Gln Gly Ser Gln Phe Ala Asp Gly Gln Arg Ala Pro Leu Ser Pro 
    130                 135                 140                 


Ser Leu Leu Ile Arg Thr Leu Gln Gly Ser Asp Lys Asn Pro Gly Glu 
145                 150                 155                 160 


Glu Lys Ala Glu Glu Glu Gly Val Ala Glu Glu Glu Gly Val Asn Lys 
                165                 170                 175     


Phe Ser Tyr Pro Pro Ser His Arg Glu Cys Cys Pro Ala Val Glu Glu 
            180                 185                 190         


Glu Asp Asp Glu Glu Ala Val Lys Lys Glu Ala His Arg Thr Ser Thr 
        195                 200                 205             


Ser Ala Leu Ser Pro Gly Ser Lys Pro Ser Thr Trp Val Ser Cys Pro 
    210                 215                 220                 


Gly Glu Glu Glu Asn Gln Ala Thr Glu Asp Lys Arg Thr Glu Arg Ser 
225                 230                 235                 240 


Lys Gly Ala Arg Lys Thr Ser Val Ser Pro Arg Ser Ser Gly Ser Asp 
                245                 250                 255     


Pro Arg Ser Trp Glu Tyr Arg Ser Gly Glu Ala Ser Glu Glu Lys Glu 
            260                 265                 270         


Glu Lys Ala His Glu Glu Thr Gly Lys Gly Glu Ala Ala Pro Gly Pro 
        275                 280                 285             


Gln Ser Ser Ala Pro Ala Gln Arg Pro Gln Leu Lys Ser Trp Trp Cys 
    290                 295                 300                 


Gln Pro Ser Asp Glu Glu Glu Ser Glu Val Lys Pro Leu Gly Ala Ala 
305                 310                 315                 320 


Glu Lys Asp Gly Glu Ala Glu Cys Pro Pro Cys Ile Pro Pro Pro Ser 
                325                 330                 335     


Ala Phe Leu Lys Ala Trp Val Tyr Trp Pro Gly Glu Asp Thr Glu Glu 
            340                 345                 350         


Glu Glu Asp Glu Glu Glu Asp Glu Asp Ser Asp Ser Gly Ser Asp Glu 
        355                 360                 365             


Glu Glu Gly Glu Ala Glu Ala Ser Ser Ser Thr Pro Ala Thr Gly Val 
    370                 375                 380                 


Phe Leu Lys Ser Trp Val Tyr Gln Pro Gly Glu Asp Thr Glu Glu Glu 
385                 390                 395                 400 


Glu Asp Glu Asp Ser Asp Thr Gly Ser Ala Glu Asp Glu Arg Glu Ala 
                405                 410                 415     


Glu Thr Ser Ala Ser Thr Pro Pro Ala Ser Ala Phe Leu Lys Ala Trp 
            420                 425                 430         


Val Tyr Arg Pro Gly Glu Asp Thr Glu Glu Glu Glu Asp Glu Asp Val 
        435                 440                 445             


Asp Ser Glu Asp Lys Glu Asp Asp Ser Glu Ala Ala Leu Gly Glu Ala 
    450                 455                 460                 


Glu Ser Asp Pro His Pro Ser His Pro Asp Gln Ser Ala His Phe Arg 
465                 470                 475                 480 


Gly Trp Gly Tyr Arg Pro Gly Lys Glu Thr Glu Glu Glu Glu Ala Ala 
                485                 490                 495     


Glu Asp Trp Gly Glu Ala Glu Pro Cys Pro Phe Arg Val Ala Ile Tyr 
            500                 505                 510         


Val Pro Gly Glu Lys Pro Pro Pro Pro Trp Ala Pro Pro Arg Leu Pro 
        515                 520                 525             


Leu Arg Leu Gln Arg Arg Leu Lys Arg Pro Glu Thr Pro Thr His Asp 
    530                 535                 540                 


Pro Asp Pro Glu Thr Pro Leu Lys Ala Arg Lys Val Arg Phe Ser Glu 
545                 550                 555                 560 


Lys Val Thr Val His Phe Leu Ala Val Trp Ala Gly Pro Ala Gln Ala 
                565                 570                 575     


Ala Arg Gln Gly Pro Trp Glu Gln Leu Ala Arg Asp Arg Ser Arg Phe 
            580                 585                 590         


Ala Arg Arg Ile Ala Gln Ala Gln Glu Glu Leu Ser Pro Cys Leu Thr 
        595                 600                 605             


Pro Ala Ala Arg Ala Arg Ala Trp Ala Arg Leu Arg Asn Pro Pro Leu 
    610                 615                 620                 


Ala Pro Ile Pro Ala Leu Thr Gln Thr Leu Pro Ser Ser Ser Val Pro 
625                 630                 635                 640 


Ser Ser Pro Val Gln Thr Thr Pro Leu Ser Gln Ala Val Ala Thr Pro 
                645                 650                 655     


Ser Arg Ser Ser Ala Ala Ala Ala Ala Ala Leu Asp Leu Ser Gly Arg 
            660                 665                 670         


Arg Gly 
        


<210>  2
<211>  12
<212>  PRT
<213>  artificial

<220>
<223>  Fragment of GADD34

<400>  2

Leu Lys Ala Arg Lys Val Arg Phe Ser Glu Lys Val 
1               5                   10          


<210>  3
<211>  12
<212>  PRT
<213>  artificial

<220>
<223>  N-terminal DPT-sh1 sequence

<400>  3

Val Lys Lys Lys Lys Ile Lys Arg Glu Ile Lys Ile 
1               5                   10          


<210>  4
<211>  24
<212>  PRT
<213>  artificial

<220>
<223>  Artificial sequence including a fragment of GADD34 and a 
       translocation motif.

<400>  4

Val Lys Lys Lys Lys Ile Lys Arg Glu Ile Lys Ile Leu Lys Ala Arg 
1               5                   10                  15      


Lys Val Arg Phe Ser Glu Lys Val 
            20                  


<210>  5
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  Sense siRNA specific for human Bax.

<400>  5
ggugccggaa cugaucagat t                                               21


<210>  6
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  Anti-sense siRNA specific for human Bax.

<400>  6
ucugaucagu uccggcacct t                                               21


<210>  7
<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  Sense siRNA specific for Bak.

<400>  7
accgacgcka tgactcagag ttc                                             23


<210>  8
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  Anti-sense siRNA specific for Bak.

<400>  8
acacggcacc aattgatg                                                   18


<210>  9
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  Sense siRNA control sequence.

<400>  9
gccgguaugc cgguuaagu                                                  19


<210>  10
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  Anti-sense siRNA control sequence.

<400>  10
acuuaaccgg cauaccggc                                                  19


<210>  11
<211>  35
<212>  DNA
<213>  artificial

<220>
<223>  Sense siRNA specific for CRT.

<400>  11
rcrcrgrcur grgrgurcrg raraurcrrr aratt                                35


<210>  12
<211>  38
<212>  DNA
<213>  artificial

<220>
<223>  siRNA specific for GADD34.

<400>  12
rcrargrgra rgrcrargra urcrargrau rargratt                             38


<210>  13
<211>  36
<212>  DNA
<213>  artificial

<220>
<223>  siRNA specific for PPICalpha.

<400>  13
rgrcurgrgr crcuraurar argraurcra rgratt                               36


<210>  14
<211>  35
<212>  DNA
<213>  artificial

<220>
<223>  siRNA control.

<400>  14
rgrcrcrgrg uraurgrcrc rgrguurara rgutt                                35


<210>  15
<211>  416
<212>  PRT
<213>  artificial

<220>
<223>  CRT protein sequence.

<400>  15

Met Leu Leu Ser Val Pro Leu Leu Leu Gly Leu Leu Gly Leu Ala Ala 
1               5                   10                  15      


Ala Asp Pro Ala Ile Tyr Phe Lys Glu Gln Phe Leu Asp Gly Asp Ala 
            20                  25                  30          


Trp Thr Asn Arg Trp Val Glu Ser Lys His Lys Ser Asp Phe Gly Lys 
        35                  40                  45              


Phe Val Leu Ser Ser Gly Lys Phe Tyr Gly Asp Leu Glu Lys Asp Lys 
    50                  55                  60                  


Gly Leu Gln Thr Ser Gln Asp Ala Arg Phe Tyr Ala Leu Ser Ala Lys 
65                  70                  75                  80  


Phe Glu Pro Phe Ser Asn Lys Gly Gly Thr Leu Val Val Gln Phe Thr 
                85                  90                  95      


Val Lys His Glu Lys Asn Ile Asp Cys Gly Gly Gly Tyr Val Lys Leu 
            100                 105                 110         


Phe Pro Ser Gly Leu Asp Gln Lys Asp Met His Gly Asp Ser Glu Tyr 
        115                 120                 125             


Asn Ile Met Phe Gly Pro Asp Ile Cys Gly Pro Gln Thr Lys Lys Val 
    130                 135                 140                 


His Val Ile Phe Asn Tyr Lys Gly Lys Asn Val Leu Ile Asn Lys Asp 
145                 150                 155                 160 


Ile Arg Cys Lys Asp Asp Glu Phe Thr His Leu Tyr Thr Leu Ile Val 
                165                 170                 175     


Arg Pro Asp Asn Thr Tyr Glu Val Lys Ile Asp Asn Ser Gln Val Glu 
            180                 185                 190         


Ser Gly Ser Leu Glu Asp Asp Trp Asp Phe Leu Pro Pro Lys Lys Ile 
        195                 200                 205             


Lys Asp Pro Asp Ala Ala Lys Pro Glu Asp Trp Asp Glu Arg Ala Lys 
    210                 215                 220                 


Ile Asp Asp Pro Thr Asp Ser Lys Pro Glu Asp Trp Asp Lys Pro Glu 
225                 230                 235                 240 


His Ile Pro Asp Pro Asp Ala Lys Lys Pro Glu Asp Trp Asp Glu Glu 
                245                 250                 255     


Met Asp Gly Glu Trp Glu Pro Pro Val Ile Gln Asn Pro Glu Tyr Lys 
            260                 265                 270         


Gly Glu Trp Lys Pro Arg Gln Ile Asp Asn Pro Asp Tyr Lys Gly Thr 
        275                 280                 285             


Trp Ile His Pro Glu Ile Asp Asn Pro Glu Tyr Ser Pro Asp Ala Asn 
    290                 295                 300                 


Ile Tyr Ala Tyr Asp Ser Phe Ala Val Leu Gly Leu Asp Leu Trp Gln 
305                 310                 315                 320 


Val Lys Ser Gly Thr Ile Phe Asp Asn Phe Leu Ile Thr Asn Asp Glu 
                325                 330                 335     


Ala Tyr Ala Glu Glu Phe Gly Asn Glu Thr Trp Gly Val Thr Lys Ala 
            340                 345                 350         


Ala Glu Lys Gln Met Lys Asp Lys Gln Asp Glu Glu Gln Arg Leu Lys 
        355                 360                 365             


Glu Glu Glu Glu Asp Lys Lys Arg Lys Glu Glu Glu Glu Ala Glu Asp 
    370                 375                 380                 


Lys Glu Asp Asp Asp Asp Arg Asp Glu Asp Glu Asp Glu Glu Asp Glu 
385                 390                 395                 400 


Lys Glu Glu Asp Glu Glu Glu Ser Pro Gly Gln Ala Lys Asp Glu Leu 
                405                 410                 415     


