                          SEQUENCE LISTING

<110>  Advisys, Inc.

<120> 	Growth Hormone Releasing Hormone Treatment to Decrease Cholesterol Levels

<130>  108328.00280- AVSI-0047

<160>  46    

<170>  PatentIn version 3.3

<210>  1
<211>  40
<212>  PRT
<213>  artificial sequence

<220>
<223>  This is a HV - growth hormone releasing hormone ("GHRH") analog.

<400>  1

His Val Asp Ala Ile Phe Thr Asn Ser Tyr Arg Lys Val Leu Ala Gln 
1               5                   10                  15      


Leu Ser Ala Arg Lys Leu Leu Gln Asp Ile Leu Asn Arg Gln Gln Gly 
            20                  25                  30          


Glu Arg Asn Gln Glu Gln Gly Ala 
        35                  40  


<210>  2
<211>  40
<212>  PRT
<213>  Artificial sequence

<220>
<223>  This is an amino-acid sequence for Pig-GHRH

<400>  2

Tyr Ala Asp Ala Ile Phe Thr Asn Ser Tyr Arg Lys Val Leu Gly Gln 
1               5                   10                  15      


Leu Ser Ala Arg Lys Leu Leu Gln Asp Ile Met Ser Arg Gln Gln Gly 
            20                  25                  30          


Glu Arg Asn Gln Glu Gln Gly Ala 
        35                  40  


<210>  3
<211>  40
<212>  PRT
<213>  artificial sequence

<220>
<223>  This is an amino-acid sequence for Bovine-GHRH

<400>  3

Tyr Ala Asp Ala Ile Phe Thr Asn Ser Tyr Arg Lys Val Leu Gly Gln 
1               5                   10                  15      


Leu Ser Ala Arg Lys Leu Leu Gln Asp Ile Met Asn Arg Gln Gln Gly 
            20                  25                  30          


Glu Arg Asn Gln Glu Gln Gly Ala 
        35                  40  


<210>  4
<211>  40
<212>  PRT
<213>  artificial sequence

<220>
<223>  This is an amino-acid sequence for Dog-GHRH

<400>  4

Tyr Ala Asp Ala Ile Phe Thr Asn Ser Tyr Arg Lys Val Leu Gly Gln 
1               5                   10                  15      


Leu Ser Ala Arg Lys Leu Leu Gln Asp Ile Met Ser Arg Gln Gln Gly 
            20                  25                  30          


Glu Arg Asn Arg Glu Gln Gly Ala 
        35                  40  


<210>  5
<211>  40
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  This is an amino-acid sequence for Cat-GHRH

<400>  5

Tyr Ala Asp Ala Ile Phe Thr Asn Ser Tyr Arg Lys Val Leu Gly Gln 
1               5                   10                  15      


Leu Ser Ala Arg Lys Leu Leu Gln Asp Ile Met Ser Arg Gln Gln Gly 
            20                  25                  30          


Glu Arg Asn Gln Glu Gln Gly Ala 
        35                  40  


<210>  6
<211>  40
<212>  PRT
<213>  artificial sequence

<220>
<223>  This is a TI - growth hormone releasing hormone ("GHRH") analog.

<400>  6

Tyr Ile Asp Ala Ile Phe Thr Asn Ser Tyr Arg Lys Val Leu Ala Gln 
1               5                   10                  15      


Leu Ser Ala Arg Lys Leu Leu Gln Asp Ile Leu Asn Arg Gln Gln Gly 
            20                  25                  30          


Glu Arg Asn Gln Glu Gln Gly Ala 
        35                  40  


<210>  7
<211>  40
<212>  PRT
<213>  artificial sequence

<220>
<223>  This is an amino-acid sequence for Ovine-GHRH

<400>  7

Tyr Ala Asp Ala Ile Phe Thr Asn Ser Tyr Arg Lys Ile Leu Gly Gln 
1               5                   10                  15      


Leu Ser Ala Arg Lys Leu Leu Gln Asp Ile Met Asn Arg Gln Gln Gly 
            20                  25                  30          


Glu Arg Asn Gln Glu Gln Gly Ala 
        35                  40  


<210>  8
<211>  43
<212>  PRT
<213>  artificial sequence

<220>
<223>  This is an amino-acid sequence for Chicken-GHRH

<400>  8

His Ala Asp Gly Ile Phe Ser Lys Ala Tyr Arg Lys Leu Leu Gly Gln 
1               5                   10                  15      


Leu Ser Ala Arg Asn Tyr Leu His Ser Leu Met Ala Lys Arg Val Gly 
            20                  25                  30          


Ser Gly Leu Gly Asp Glu Ala Glu Pro Leu Ser 
        35                  40              


<210>  9
<211>  40
<212>  PRT
<213>  artificial sequence

<220>
<223>  This is an amino-acid sequence for Horse GHRH. 

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  "X" can be any AA sequence


<220>
<221>  misc_feature
<222>  (30)..(40)
<223>  "X" can be any AA sequence


<400>  9

Xaa Ala Asp Ala Ile Phe Thr Asn Asn Tyr Arg Lys Val Leu Gly Gln 
1               5                   10                  15      


Leu Ser Ala Arg Lys Ile Leu Gln Asp Ile Met Ser Arg Xaa Xaa Xaa 
            20                  25                  30          


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
        35                  40  


<210>  10
<211>  3
<212>  PRT
<213>  artificial sequence

<220>
<223>  This is an amino-acid sequence for human (1-40) GHRH

<400>  10

Tyr Ala Asp Ala Ile Phe Thr Asn Ser Tyr Arg Lys Val Leu Gly Gln 
1               5                   10                  15      


Leu Ser Ala Arg Lys Leu Leu Gln Asp Ile Met Ser Arg Gln Gln Gly 
            20                  25                  30          


Glu Ser Asn Gln Glu Arg Gly Ala
35 40


<210>  11
<211>  40
<212>  PRT
<213>  artificial sequence

<220>
<223>  This is a TV - growth hormone releasing hormone ("GHRH") analog.

<400>  11

Tyr Val Asp Ala Ile Phe Thr Asn Ser Tyr Arg Lys Val Leu Ala Gln 
1               5                   10                  15      


Leu Ser Ala Arg Lys Leu Leu Gln Asp Ile Leu Asn Arg Gln Gln Gly 
            20                  25                  30          


Glu Arg Asn Gln Glu Gln Gly Ala 
        35                  40  


<210>  12
<211>  40
<212>  PRT
<213>  artificial sequence

<220>
<223>  This is a TA - growth hormone releasing hormone ("GHRH") analog.

<400>  12

Tyr Ala Asp Ala Ile Phe Thr Asn Ser Tyr Arg Lys Val Leu Ala Gln 
1               5                   10                  15      


Leu Ser Ala Arg Lys Leu Leu Gln Asp Ile Leu Asn Arg Gln Gln Gly 
            20                  25                  30          


Glu Arg Asn Gln Glu Gln Gly Ala 
        35                  40  


<210>  13
<211>  44
<212>  PRT
<213>  artificial sequence

<220>
<223>  This is a human (1-44) growth hormone releasing hormone ("GHRH").

<400>  13

Tyr Ala Asp Ala Ile Phe Thr Asn Ser Tyr Arg Lys Val Leu Gly Gln 
1               5                   10                  15      


Leu Ser Ala Arg Lys Leu Leu Gln Asp Ile Met Ser Arg Gln Gln Gly 
            20                  25                  30          


Glu Ser Asn Gln Glu Arg Gly Ala Arg Ala Arg Leu 
        35                  40                  


<210>  14
<211>  40
<212>  PRT
<213>  artificial sequence

<220>
<223>  This is the artificial sequence for GHRH (1-40)OH.

<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa at position 1 may be tyrosine, or histidine

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 may be alanine, valine, or isoleucine.

<220>
<221>  MISC_FEATURE
<222>  (15)..(15)
<223>  Xaa at position 15 may be alanine, valine, or isoleucine.

<220>
<221>  MISC_FEATURE
<222>  (27)..(27)
<223>  Xaa at position 27 may be methionine, or leucine. 

<220>
<221>  MISC_FEATURE
<222>  (28)..(28)
<223>  Xaa at position 28 may be serine or asparagine.

<220>
<221>  MISC_FEATURE
<222>  (34)..(34)
<223>  Xaa at position 2 may be arginine or serine.

<220>
<221>  MISC_FEATURE
<222>  (36)..(36)
<223>  Xaa at position 2 may be arginine or glutamine.

<220>
<221>  MISC_FEATURE
<222>  (38)..(38)
<223>  Xaa at position 2 may be arginine or glutamine.



<400>  14

Xaa Xaa Asp Ala Ile Phe Thr Asn Ser Tyr Arg Lys Val Leu Xaa Gln 
1               5                   10                  15      


Leu Ser Ala Arg Lys Leu Leu Gln Asp Ile Xaa Xaa Arg Gln Gln Gly 
            20                  25                  30          


Glu Xaa Asn Xaa Glu Xaa Gly Ala 
        35                  40  


<210>  15
<211>  323
<212>  DNA
<213>  artificial sequence

<220>
<223>  This is a nucleic acid sequence of a eukaryotic promoter c5-12.  

<400>  15
cggccgtccg ccctcggcac catcctcacg acacccaaat atggcgacgg gtgaggaatg     60

gtggggagtt atttttagag cggtgaggaa ggtgggcagg cagcaggtgt tggcgctcta    120

aaaataactc ccgggagtta tttttagagc ggaggaatgg tggacaccca aatatggcga    180

cggttcctca cccgtcgcca tatttgggtg tccgccctcg gccggggccg cattcctggg    240

ggccgggcgg tgctcccgcc cgcctcgata aaaggctccg gggccggcgg cggcccacga    300

gctacccgga ggagcgggag gcg                                            323


<210>  16
<211>  190
<212>  DNA
<213>  artificial sequence

<220>
<223>  Nucleic acid sequence of a human growth hormone poly A tail.  

<400>  16
gggtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt tgccactcca     60

gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc    120

ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca    180

acctgtaggg                                                           190



<210>  17
<211>  795
<212>  DNA
<213>  artificial sequence

<220>
<223>  Nucleic acid sequence for the antibiotic resistance gene 
	kanamycin.

<400>  17
atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc     60

ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca    120

gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg    180

caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg    240

ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag    300

gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg    360

cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc    420

atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa    480

gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac    540

ggcgaggatc tcgtcgtgac tcatggcgat gcctgcttgc cgaatatcat ggtggaaaat    600

ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac    660

atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc    720

ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt    780

gacgagttct tctga                                                     795


<210>  18
<211>  55
<212>  DNA
<213>  artificial sequence

<220>
<223>  Nucleic acid sequence of human growth hormone 5'untranslated 
	region  

<400>  18
caaggcccaa ctccccgaac cactcagggt cctgtggaca gctcacctag ctgcc          55


<210>  19
<211>  782
<212>  DNA
<213>  artificial sequence

<220>
<223>  Nucleic acid sequence of a plasmid pUC-18 origin of replication

<400>  19
tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta     60

tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag    120

aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg    180

tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg    240

tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg    300

cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga    360

agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc    420

tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt    480

aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact    540

ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg    600

cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt    660

accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt    720

ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct    780

tt                                                                   782


<210>  20
<211>  5
<212>  DNA
<213>  artificial sequence

<220>
<223>  This is a NEO ribosomal binding site

<400>  20
tcctc                                                                  5


<210>  21
<211>  29
<212>  DNA
<213>  artificial sequence

<220>
<223>  Nucleic acid sequence of a prokaryotic PNEO promoter. 

<400>  21
accttaccag agggcgcccc agctggcaa                                       29


<210>  22
<211>  3534
<212>  DNA
<213>  artificial sequence

<220>
<223>  Plasmid vector having an analog HV-GHRH sequence.

<400>  22
gttgtaaaac gacggccagt gaattgtaat acgactcact atagggcgaa ttggagctcc     60

accgcggtgg cggccgtccg ccctcggcac catcctcacg acacccaaat atggcgacgg    120

gtgaggaatg gtggggagtt atttttagag cggtgaggaa ggtgggcagg cagcaggtgt    180

tggcgctcta aaaataactc ccgggagtta tttttagagc ggaggaatgg tggacaccca    240

aatatggcga cggttcctca cccgtcgcca tatttgggtg tccgccctcg gccggggccg    300

cattcctggg ggccgggcgg tgctcccgcc cgcctcgata aaaggctccg gggccggcgg    360

cggcccacga gctacccgga ggagcgggag gcgccaagct ctagaactag tggatcccaa    420

ggcccaactc cccgaaccac tcagggtcct gtggacagct cacctagctg ccatggtgct    480

ctgggtgttc ttctttgtga tcctcaccct cagcaacagc tcccactgct ccccacctcc    540

ccctttgacc ctcaggatgc ggcggcacgt agatgccatc ttcaccaaca gctaccggaa    600

ggtgctggcc cagctgtccg cccgcaagct gctccaggac atcctgaaca ggcagcaggg    660

agagaggaac caagagcaag gagcataatg actgcaggaa ttcgatatca agcttatcgg    720

ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cctggaagtt gccactccag    780

tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac taggtgtcct    840

tctataatat tatggggtgg aggggggtgg tatggagcaa ggggcaagtt gggaagacaa    900

cctgtagggc ctgcggggtc tattgggaac caagctggag tgcagtggca caatcttggc    960

tcactgcaat ctccgcctcc tgggttcaag cgattctcct gcctcagcct cccgagttgt   1020

tgggattcca ggcatgcatg accaggctca gctaattttt gtttttttgg tagagacggg   1080

gtttcaccat attggccagg ctggtctcca actcctaatc tcaggtgatc tacccacctt   1140

ggcctcccaa attgctggga ttacaggcgt gaaccactgc tcccttccct gtccttctga   1200

ttttaaaata actataccag caggaggacg tccagacaca gcataggcta cctggccatg   1260

cccaaccggt gggacatttg agttgcttgc ttggcactgt cctctcatgc gttgggtcca   1320

ctcagtagat gcctgttgaa ttcgataccg tcgacctcga gggggggccc ggtaccagct   1380

tttgttccct ttagtgaggg ttaatttcga gcttggcgta atcatggtca tagctgtttc   1440

ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt   1500

gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc   1560

ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg   1620

ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct   1680

cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca   1740

cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga   1800

accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc   1860

acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg   1920

cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat   1980

acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt   2040

atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc   2100

agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg   2160

acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg   2220

gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg   2280

gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg   2340

gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca   2400

gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagaaga   2460

actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa   2520

gcacgaggaa gcggtcagcc cattcgccgc caagctcttc agcaatatca cgggtagcca   2580

acgctatgtc ctgatagcgg tccgccacac ccagccggcc acagtcgatg aatccagaaa   2640

agcggccatt ttccaccatg atattcggca agcaggcatc gccatgggtc acgacgagat   2700

cctcgccgtc gggcatgcgc gccttgagcc tggcgaacag ttcggctggc gcgagcccct   2760

gatgctcttc gtccagatca tcctgatcga caagaccggc ttccatccga gtacgtgctc   2820

gctcgatgcg atgtttcgct tggtggtcga atgggcaggt agccggatca agcgtatgca   2880

gccgccgcat tgcatcagcc atgatggata ctttctcggc aggagcaagg tgagatgaca   2940

ggagatcctg ccccggcact tcgcccaata gcagccagtc ccttcccgct tcagtgacaa   3000

cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct   3060

cgtcctgcag ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc   3120

cctgcgctga cagccggaac acggcggcat cagagcagcc gattgtctgt tgtgcccagt   3180

catagccgaa tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt   3240

caatcatgcg aaacgatcct catcctgtct cttgatcaga tcttgatccc ctgcgccatc   3300

agatccttgg cggcaagaaa gccatccagt ttactttgca gggcttccca accttaccag   3360

agggcgcccc agctggcaat tccggttcgc ttgctgtcca taaaaccgcc cagtctagca   3420

actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg   3480

gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgac         3534


<210>  23
<211>  2722
<212>  DNA
<213>  artificial sequence

<220>
<223>  Plasmid vector having a codon optimized mouse GHRH sequence

<400>  23
ccaccgcggt ggcggccgtc cgccctcggc accatcctca cgacacccaa atatggcgac     60

gggtgaggaa tggtggggag ttatttttag agcggtgagg aaggtgggca ggcagcaggt    120

gttggcgctc taaaaataac tcccgggagt tatttttaga gcggaggaat ggtggacacc    180

caaatatggc gacggttcct cacccgtcgc catatttggg tgtccgccct cggccggggc    240

cgcattcctg ggggccgggc ggtgctcccg cccgcctcga taaaaggctc cggggccggc    300

ggcggcccac gagctacccg gaggagcggg aggcgccaag cggatcccaa ggcccaactc    360

cccgaaccac tcagggtcct gtggacagct cacctagctg ccatggtgct ctgggtgctc    420

tttgtgatcc tcatcctcac cagcggcagc cactgcagcc tgcctcccag ccctcccttc    480

aggatgcaga ggcacgtgga cgccatcttc accaccaact acaggaagct gctgagccag    540

ctgtacgcca ggaaggtgat ccaggacatc atgaacaagc agggcgagag gatccaggag    600

cagagggcca ggctgagctg ataagcttat cggggtggca tccctgtgac ccctccccag    660

tgcctctcct ggccctggaa gttgccactc cagtgcccac cagccttgtc ctaataaaat    720

taagttgcat cattttgtct gactaggtgt ccttctataa tattatgggg tggagggggg    780

tggtatggag caaggggcaa gttgggaaga caacctgtag ggctcgaggg ggggcccggt    840

accagctttt gttcccttta gtgagggtta atttcgagct tggtcttccg cttcctcgct    900

cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc    960

ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg   1020

ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg   1080

cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg   1140

actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac   1200

cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca   1260

tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt   1320

gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc   1380

caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag   1440

agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac   1500

tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt   1560

tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa   1620

gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg   1680

gtctgacgct cagctagcgc tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc   1740

tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc ggtcagccca ttcgccgcca   1800

agctcttcag caatatcacg ggtagccaac gctatgtcct gatagcggtc cgccacaccc   1860

agccggccac agtcgatgaa tccagaaaag cggccatttt ccaccatgat attcggcaag   1920

caggcatcgc catgagtcac gacgagatcc tcgccgtcgg gcatgcgcgc cttgagcctg   1980

gcgaacagtt cggctggcgc gagcccctga tgctcttcgt ccagatcatc ctgatcgaca   2040

agaccggctt ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat   2100

gggcaggtag ccggatcaag cgtatgcagc cgccgcattg catcagccat gatggatact   2160

ttctcggcag gagcaaggtg agatgacagg agatcctgcc ccggcacttc gcccaatagc   2220

agccagtccc ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc   2280

gtggccagcc acgatagccg cgctgcctcg tcctgcagtt cattcagggc accggacagg   2340

tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca gccggaacac ggcggcatca   2400

gagcagccga ttgtctgttg tgcccagtca tagccgaata gcctctccac ccaagcggcc   2460

ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa acgatcctca tcctgtctct   2520

tgatcagatc ttgatcccct gcgccatcag atccttggcg gcaagaaagc catccagttt   2580

actttgcagg gcttcccaac cttaccagag ggcgccccag ctggcaattc cggttcgctt   2640

gctgtccata aaaccgccca gtctagcaac tgttgggaag ggcgatcgtg taatacgact   2700

cactataggg cgaattggag ct                                            2722


<210>  24
<211>  2725
<212>  DNA
<213>  artificial sequence

<220>
<223>  Plasmid vector having a codon optimized rat GHRH sequence

<400>  24
ccaccgcggt ggcggccgtc cgccctcggc accatcctca cgacacccaa atatggcgac     60

gggtgaggaa tggtggggag ttatttttag agcggtgagg aaggtgggca ggcagcaggt    120

gttggcgctc taaaaataac tcccgggagt tatttttaga gcggaggaat ggtggacacc    180

caaatatggc gacggttcct cacccgtcgc catatttggg tgtccgccct cggccggggc    240

cgcattcctg ggggccgggc ggtgctcccg cccgcctcga taaaaggctc cggggccggc    300

ggcggcccac gagctacccg gaggagcggg aggcgccaag cggatcccaa ggcccaactc    360

cccgaaccac tcagggtcct gtggacagct cacctagctg ccatggccct gtgggtgttc    420

ttcgtgctgc tgaccctgac cagcggaagc cactgcagcc tgcctcccag ccctcccttc    480

agggtgcgcc ggcacgccga cgccatcttc accagcagct acaggaggat cctgggccag    540

ctgtacgcta ggaagctcct gcacgagatc atgaacaggc agcagggcga gaggaaccag    600

gagcagagga gcaggttcaa ctgataagct tatcggggtg gcatccctgt gacccctccc    660

cagtgcctct cctggccctg gaagttgcca ctccagtgcc caccagcctt gtcctaataa    720

aattaagttg catcattttg tctgactagg tgtccttcta taatattatg gggtggaggg    780

gggtggtatg gagcaagggg caagttggga agacaacctg tagggctcga gggggggccc    840

ggtaccagct tttgttccct ttagtgaggg ttaatttcga gcttggtctt ccgcttcctc    900

gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa    960

ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa   1020

aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct   1080

ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac   1140

aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc   1200

gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc   1260

tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg   1320

tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga   1380

gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag   1440

cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta   1500

cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag   1560

agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg   1620

caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac   1680

ggggtctgac gctcagctag cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg   1740

cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg   1800

ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca   1860

cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc   1920

aagcaggcat cgccatgagt cacgacgaga tcctcgccgt cgggcatgcg cgccttgagc   1980

ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg   2040

acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg   2100

aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat   2160

actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat   2220

agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc   2280

gtcgtggcca gccacgatag ccgcgctgcc tcgtcctgca gttcattcag ggcaccggac   2340

aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca   2400

tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg   2460

gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc   2520

tcttgatcag atcttgatcc cctgcgccat cagatccttg gcggcaagaa agccatccag   2580

tttactttgc agggcttccc aaccttacca gagggcgccc cagctggcaa ttccggttcg   2640

cttgctgtcc ataaaaccgc ccagtctagc aactgttggg aagggcgatc gtgtaatacg   2700

actcactata gggcgaattg gagct                                         2725



<210>  25
<211>  2725
<212>  DNA
<213>  artificial sequence

<220>
<223>  This is the codon optimized HV-GHRH expression plasmid.

<400>  25
ccaccgcggt ggcggccgtc cgccctcggc accatcctca cgacacccaa atatggcgac     60

gggtgaggaa tggtggggag ttatttttag agcggtgagg aaggtgggca ggcagcaggt    120

gttggcgctc taaaaataac tcccgggagt tatttttaga gcggaggaat ggtggacacc    180

caaatatggc gacggttcct cacccgtcgc catatttggg tgtccgccct cggccggggc    240

cgcattcctg ggggccgggc ggtgctcccg cccgcctcga taaaaggctc cggggccggc    300

ggcggcccac gagctacccg gaggagcggg aggcgccaag cggatcccaa ggcccaactc    360

cccgaaccac tcagggtcct gtggacagct cacctagctg ccatggtgct ctgggtgttc    420

ttctttgtga tcctcaccct cagcaacagc tcccactgct ccccacctcc ccctttgacc    480

ctcaggatgc ggcggcacgt agatgccatc ttcaccaaca gctaccggaa ggtgctggcc    540

cagctgtccg cccgcaagct gctccaggac atcctgaaca ggcagcaggg agagaggaac    600

caagagcaag gagcataatg acatcaagct tatcggggtg gcatccctgt gacccctccc    660

cagtgcctct cctggccctg gaagttgcca ctccagtgcc caccagcctt gtcctaataa    720

aattaagttg catcattttg tctgactagg tgtccttcta taatattatg gggtggaggg    780

gggtggtatg gagcaagggg caagttggga agacaacctg tagggctcga gggggggccc    840

ggtaccagct tttgttccct ttagtgaggg ttaatttcga gcttggtctt ccgcttcctc    900

gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa    960

ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa   1020

aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct   1080

ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac   1140

aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc   1200

gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc   1260

tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg   1320

tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga   1380

gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag   1440

cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta   1500

cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag   1560

agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgttta   1620

caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac   1680

ggggtctgac gctcagctag cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg   1740

cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg   1800

ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca   1860

cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc   1920

aagcaggcat cgccatgagt cacgacgaga tcctcgccgt cgggcatgcg cgccttgagc   1980

ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg   2040

acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg   2100

aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat   2160

actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat   2220

agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc   2280

gtcgtggcca gccacgatag ccgcgctgcc tcgtcctgca gttcattcag ggcaccggac   2340

aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca   2400

tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg   2460

gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc   2520

tcttgatcag atcttgatcc cctgcgccat cagatccttg gcggcaagaa agccatccag   2580

tttactttgc agggcttccc aaccttacca gagggcgccc cagctggcaa ttccggttcg   2640

cttgctgtcc ataaaaccgc ccagtctagc aactgttggg aagggcgatc gtgtaatacg   2700

actcactata gggcgaattg gagct                                         2725


<210>  26
<211>  2505
<212>  DNA
<213>  artificial sequence

<220>
<223>  This is the codon optimized pig-GHRH expression plasmid.

<400>  26
ccaccgcggt ggcggccgtc cgccctcggc accatcctca cgacacccaa atatggcgac     60

gggtgaggaa tggtggggag ttatttttag agcggtgagg aaggtgggca ggcagcaggt    120

gttggcgctc taaaaataac tcccgggagt tatttttaga gcggaggaat ggtggacacc    180

caaatatggc gacggttcct cacccgtcgc catatttggg tgtccgccct cggccggggc    240

cgcattcctg ggggccgggc ggtgctcccg cccgcctcga taaaaggctc cggggccggc    300

ggcggcccac gagctacccg gaggagcggg aggcgccaag cggatcccaa ggcccaactc    360

cccgaaccac tcagggtcct gtggacagct cacctagctg ccatggtgct ctgggtgttc    420

ttctttgtga tcctcaccct cagcaacagc tcccactgct ccccacctcc ccctttgacc    480

ctcaggatgc ggcggtatgc agatgccatc ttcaccaaca gctaccggaa ggtgctgggc    540

cagctgtccg cccgcaagct gctccaggac atcatgagca ggcagcaggg agagaggaac    600

caagagcaag gagcataatg aaagcttatc ggggtggcat ccctgtgacc cctccccagt    660

gcctctcctg gccctggaag ttgccactcc agtgcccacc agccttgtcc taataaaatt    720

aagttgcatc attttgtctg actaggtgtc cttctataat attatggggt ggaggggggt    780

ggtatggagc aaggggcaag ttgggaagac aacctgtagg gctcgagggg gggcccggta    840

ccatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt    900

ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt    960

ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc   1020

gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa   1080

gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct   1140

ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta   1200

actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg   1260

gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc   1320

ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta   1380

ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg   1440

gtttttttgt ttacaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt   1500

tgatcttttc tacggggtct gacgctcagc tagcgctcag aagaactcgt caagaaggcg   1560

atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc   1620

agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata   1680

gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac   1740

catgatattc ggcaagcagg catcgccatg agtcacgacg agatcctcgc cgtcgggcat   1800

gcgcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag   1860

atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt   1920

cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc   1980

agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg   2040

cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc   2100

gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct gcagttcatt   2160

cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg   2220

gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct   2280

ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga   2340

tcctcatcct gtctcttgat cagatcttga tcccctgcgc catcagatcc ttggcggcaa   2400

gaaagccatc cagtttactt tgcagggctt cccaacctta ccagagggcg ccccagctgg   2460

caattccggt tcgcttgctg tccataaaac cgcccagtct gagct			 2505


<210>  27
<211>  2716
<212>  DNA
<213>  artificial sequence

<220>
<223>  This is the codon optimized dog-GHRH expression plasmid.

<400>  27
ccaccgcggt ggcggccgtc cgccctcggc accatcctca cgacacccaa atatggcgac     60

gggtgaggaa tggtggggag ttatttttag agcggtgagg aaggtgggca ggcagcaggt    120

gttggcgctc taaaaataac tcccgggagt tatttttaga gcggaggaat ggtggacacc    180

caaatatggc gacggttcct cacccgtcgc catatttggg tgtccgccct cggccggggc    240

cgcattcctg ggggccgggc ggtgctcccg cccgcctcga taaaaggctc cggggccggc    300

ggcggcccac gagctacccg gaggagcggg aggcgccaag cggatcccaa ggcccaactc    360

cccgaaccac tcagggtcct gtggacagct cacctagctg ccatggtgct ctgggtgttc    420

ttcctggtga tcctcaccct cagcagtggt tcccactctt ccccgccatc cctgcccatc    480

agaatccctc ggtatgcaga cgccatcttc accaacagct accggaaggt gctgggccag    540

ctgtccgccc gcaagctcct scaggacatc atgagccggc agcagggaga gagaaaccgg    600

gagcaaggag catagtaagc ttatcggggt ggcatccctg tgacccctcc ccagtgcctc    660

tcctggccct ggaagttgcc actccagtgc ccaccagcct tgtcctaata aaattaagtt    720

gcatcatttt gtctgactag gtgtccttct ataatattat ggggtggagg ggggtggtat    780

ggagcaaggg gcaagttggg aagacaacct gtagggctcg agggggggcc cggtaccagc    840

ttttgttccc tttagtgagg gttaatttcg agcttggtct tccgcttcct cgctcactga    900

ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat    960

acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca   1020

aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc   1080

tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata   1140

aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc   1200

gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc   1260

acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga   1320

accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc   1380

ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag   1440

gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag   1500

aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag   1560

ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt acaagcagca   1620

gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga   1680

cgctcagcta gcgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa   1740

tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct   1800

tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg   1860

ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca   1920

tcgccatgag tcacgacgag atcctcgccg tcgggcatgc gcgccttgag cctggcgaac   1980

agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg   2040

gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag   2100

gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg   2160

gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag   2220

tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc   2280

agccacgata gccgcgctgc ctcgtcctgc agttcattca gggcaccgga caggtcggtc   2340

ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag   2400

ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa   2460

cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca   2520

gatcttgatc ccctgcgcca tcagatcctt ggcggcaaga aagccatcca gtttactttg   2580

cagggcttcc caaccttacc agagggcgcc ccagctggca attccggttc gcttgctgtc   2640

cataaaaccg cccagtctag caactgttgg gaagggcgat cgtgtaatac gactcactat   2700

agggcgaatt ggagct                                                   2716


<210>  28
<211>  2716
<212>  DNA
<213>  artificial sequence

<220>
<223>  This is the codon optimized bovine-GHRH expression plasmid.

<400>  28
ccaccgcggt ggcggccgtc cgccctcggc accatcctca cgacacccaa atatggcgac     60

gggtgaggaa tggtggggag ttatttttag agcggtgagg aaggtgggca ggcagcaggt    120

gttggcgctc taaaaataac tcccgggagt tatttttaga gcggaggaat ggtggacacc    180

caaatatggc gacggttcct cacccgtcgc catatttggg tgtccgccct cggccggggc    240

cgcattcctg ggggccgggc ggtgctcccg cccgcctcga taaaaggctc cggggccggc    300

ggcggcccac gagctacccg gaggagcggg aggcgccaag cggatcccaa ggcccaactc    360

cccgaaccac tcagggtcct gtggacagct cacctagctg ccatggtgct gtgggtgttc    420

ttcctggtga ccctgaccct gagcagcggc tcccacggct ccctgccctc ccagcctctg    480

cgcatccctc gctacgccga cgccatcttc accaacagct accgcaaggt gctcggccag    540

ctcagcgccc gcaagctcct gcaggacatc atgaaccggc agcagggcga gcgcaaccag    600

gagcagggag cctgataagc ttatcggggt ggcatccctg tgacccctcc ccagtgcctc    660

tcctggccct ggaagttgcc actccagtgc ccaccagcct tgtcctaata aaattaagtt    720

gcatcatttt gtctgactag gtgtccttct ataatattat ggggtggagg ggggtggtat    780

ggagcaaggg gcaagttggg aagacaacct gtagggctcg agggggggcc cggtaccagc    840

ttttgttccc tttagtgagg gttaatttcg agcttggtct tccgcttcct cgctcactga    900

ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat    960

acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca   1020

aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc   1080

tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata   1140

aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc   1200

gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc   1260

acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga   1320

accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc   1380

ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag   1440

gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag   1500

aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag   1560

ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt acaagcagca   1620

gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga   1680

cgctcagcta gcgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa   1740

tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct   1800

tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg   1860

ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca   1920

tcgccatgag tcacgacgag atcctcgccg tcgggcatgc gcgccttgag cctggcgaac   1980

agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg   2040

gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag   2100

gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg   2160

gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag   2220

tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc   2280

agccacgata gccgcgctgc ctcgtcctgc agttcattca gggcaccgga caggtcggtc   2340

ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag   2400

ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa   2460

cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca   2520

gatcttgatc ccctgcgcca tcagatcctt ggcggcaaga aagccatcca gtttactttg   2580

cagggcttcc caaccttacc agagggcgcc ccagctggca attccggttc gcttgctgtc   2640

cataaaaccg cccagtctag caactgttgg gaagggcgat cgtgtaatac gactcactat   2700

agggcgaatt ggagct                                                   2716


<210>  29
<211>  2716
<212>  DNA
<213>  artificial sequence

<220>
<223>  This is the codon optimized cat-GHRH expression plasmid.

<400>  29
ccaccgcggt ggcggccgtc cgccctcggc accatcctca cgacacccaa atatggcgac     60

gggtgaggaa tggtggggag ttatttttag agcggtgagg aaggtgggca ggcagcaggt    120

gttggcgctc taaaaataac tcccgggagt tatttttaga gcggaggaat ggtggacacc    180

caaatatggc gacggttcct cacccgtcgc catatttggg tgtccgccct cggccggggc    240

cgcattcctg ggggccgggc ggtgctcccg cccgcctcga taaaaggctc cggggccggc    300

ggcggcccac gagctacccg gaggagcggg aggcgccaag cggatcccaa ggcccaactc    360

cccgaaccac tcagggtcct gtggacagct cacctagctg ccatggtgct ctgggtgttc    420

ttcctggtga tcctcacccs ssacagtggc tcccactgct ccccgccatc cctgcccctc    480

agaatgcctc ggtatgcaga tgccatcttc accaacagct accggaaggt gctgggtcag    540

ctgtctgccc gcaagctact gcaggacatc atgagcagac agcagggaga gagaaaccag    600

gagcaaggag cataataagc ttatcggggt ggcatccctg tgacccctcc ccagtgcctc    660

tcctggccct ggaagttgcc actccagtgc ccaccagcct tgtcctaata aaattaagtt    720

gcatcatttt gtctgactag gtgtccttct ataatattat ggggtggagg ggggtggtat    780

ggagcaaggg gcaagttggg aagacaacct gtagggctcg agggggggcc cggtaccagc    840

ttttgttccc tttagtgagg gttaatttcg agcttggtct tccgcttcct cgctcactga    900

ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat    960

acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca   1020

aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc   1080

tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata   1140

aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc   1200

gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc   1260

acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga   1320

accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc   1380

ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag   1440

gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag   1500

aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag   1560

ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt acaagcagca   1620

gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga   1680

cgctcagcta gcgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa   1740

tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct   1800

tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg   1860

ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca   1920

tcgccatgag tcacgacgag atcctcgccg tcgggcatgc gcgccttgag cctggcgaac   1980

agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg   2040

gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag   2100

gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg   2160

gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag   2220

tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc   2280

agccacgata gccgcgctgc ctcgtcctgc agttcattca gggcaccgga caggtcggtc   2340

ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag   2400

ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa   2460

cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca   2520

gatcttgatc ccctgcgcca tcagatcctt ggcggcaaga aagccatcca gtttactttg   2580

cagggcttcc caaccttacc agagggcgcc ccagctggca attccggttc gcttgctgtc   2640

cataaaaccg cccagtctag caactgttgg gaagggcgat cgtgtaatac gactcactat   2700

agggcgaatt ggagct                                                   2716


<210>  30
<211>  2739
<212>  DNA
<213>  artificial sequence 

<220>
<223>  This is the codon optimized TI-GHRH expression plasmid.

<400>  30
ccaccgcggt ggcggccgtc cgccctcggc accatcctca cgacacccaa atatggcgac     60

gggtgaggaa tggtggggag ttatttttag agcggtgagg aaggtgggca ggcagcaggt    120

gttggcgctc taaaaataac tcccgggagt tatttttaga gcggaggaat ggtggacacc    180

caaatatggc gacggttcct cacccgtcgc catatttggg tgtccgccct cggccggggc    240

cgcattcctg ggggccgggc ggtgctcccg cccgcctcga taaaaggctc cggggccggc    300

ggcggcccac gagctacccg gaggagcggg aggcgccaag cggatcccaa ggcccaactc    360

cccgaaccac tcagggtcct gtggacagct cacctagctg ccatggtgct ctgggtgttc    420

ttctttgtga tcctcaccct cagcaacagc tcccactgct ccccacctcc ccctttgacc    480

ctcaggatgc ggcggtatat cgatgccatc ttcaccaaca gctaccggaa ggtgctggcc    540

cagctgtccg cccgcaagct gctccaggac atcctgaaca ggcagcaggg agagaggaac    600

caagagcaag gagcataatg actgcaggaa ttcgatatca agcttatcgg ggtggcatcc    660

ctgtgacccc tccccagtgc ctctcctggc cctggaagtt gccactccag tgcccaccag    720

ccttgtccta ataaaattaa gttgcatcat tttgtctgac taggtgtcct tctataatat    780

tatggggtgg aggggggtgg tatggagcaa ggggcaagtt gggaagacaa cctgtagggc    840

tcgagggggg gcccggtacc agcttttgtt ccctttagtg agggttaatt tcgagcttgg    900

tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta    960

tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag   1020

aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg   1080

tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg   1140

tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg   1200

cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga   1260

agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc   1320

tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt   1380

aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact   1440

ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg   1500

cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt   1560

accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt   1620

ggtttttttg tttacaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct   1680

ttgatctttt ctacggggtc tgacgctcag ctagcgctca gaagaactcg tcaagaaggc   1740

gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg aggaagcggt   1800

cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct atgtcctgat   1860

agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg ccattttcca   1920

ccatgatatt cggcaagcag gcatcgccat gagtcacgac gagatcctcg ccgtcgggca   1980

tgcgcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca   2040

gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt   2100

tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc cgcattgcat   2160

cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga tcctgccccg   2220

gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg agcacagctg   2280

cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtcc tgcagttcat   2340

tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc   2400

ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag ccgaatagcc   2460

tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg   2520

atcctcatcc tgtctcttga tcagatcttg atcccctgcg ccatcagatc cttggcggca   2580

agaaagccat ccagtttact ttgcagggct tcccaacctt accagagggc gccccagctg   2640

gcaattccgg ttcgcttgct gtccataaaa ccgcccagtc tagcaactgt tgggaagggc   2700

gatcgtgtaa tacgactcac tatagggcga attggagct                          2739


<210>  31
<211>  2716
<212>  DNA
<213>  artificial sequence

<220>
<223>  This is the codon optimized ovine-GHRH expression plasmid.

<400>  31
ccaccgcggt ggcggccgtc cgccctcggc accatcctca cgacacccaa atatggcgac     60

gggtgaggaa tggtggggag ttatttttag agcggtgagg aaggtgggca ggcagcaggt    120

gttggcgctc taaaaataac tcccgggagt tatttttaga gcggaggaat ggtggacacc    180

caaatatggc gacggttcct cacccgtcgc catatttggg tgtccgccct cggccggggc    240

cgcattcctg ggggccgggc ggtgctcccg cccgcctcga taaaaggctc cggggccggc    300

ggcggcccac gagctacccg gaggagcggg aggcgccaag cggatcccaa ggcccaactc    360

cccgaaccac tcagggtcct gtggacagct cacctagctg ccatggtgct gtgggtgttc    420

ttcctggtga ccctgaccct gagcagcgga agccacggca gcctgcccag ccagcccctg    480

aggatcccta ggtacgccga cgccatcttc accaacagct acaggaagat cctgggccag    540

ctgagcgcta ggaagctcct gcaggacatc atgaacaggc agcagggcga gaggaaccag    600

gagcagggcg cctgataagc ttatcggggt ggcatccctg tgacccctcc ccagtgcctc    660

tcctggccct ggaagttgcc actccagtgc ccaccagcct tgtcctaata aaattaagtt    720

gcatcatttt gtctgactag gtgtccttct ataatattat ggggtggagg ggggtggtat    780

ggagcaaggg gcaagttggg aagacaacct gtagggctcg agggggggcc cggtaccagc    840

ttttgttccc tttagtgagg gttaatttcg agcttggtct tccgcttcct cgctcactga    900

ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat    960

acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca   1020

aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc   1080

tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata   1140

aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc   1200

gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc   1260

acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga   1320

accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc   1380

ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag   1440

gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag   1500

aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag   1560

ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt acaagcagca   1620

gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga   1680

cgctcagcta gcgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa   1740

tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct   1800

tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg   1860

ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca   1920

tcgccatgag tcacgacgag atcctcgccg tcgggcatgc gcgccttgag cctggcgaac   1980

agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg   2040

gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag   2100

gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg   2160

gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag   2220

tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc   2280

agccacgata gccgcgctgc ctcgtcctgc agttcattca gggcaccgga caggtcggtc   2340

ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag   2400

ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa   2460

cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca   2520

gatcttgatc ccctgcgcca tcagatcctt ggcggcaaga aagccatcca gtttactttg   2580

cagggcttcc caaccttacc agagggcgcc ccagctggca attccggttc gcttgctgtc   2640

cataaaaccg cccagtctag caactgttgg gaagggcgat cgtgtaatac gactcactat   2700

agggcgaatt ggagct                                                   2716


<210>  32
<211>  2725
<212>  DNA
<213>  artificial sequence

<220>
<223>  This is the optimized chicken-GHRH expression plasmid.

<400>  32
ccaccgcggt ggcggccgtc cgccctcggc accatcctca cgacacccaa atatggcgac     60

gggtgaggaa tggtggggag ttatttttag agcggtgagg aaggtgggca ggcagcaggt    120

gttggcgctc taaaaataac tcccgggagt tatttttaga gcggaggaat ggtggacacc    180

caaatatggc gacggttcct cacccgtcgc catatttggg tgtccgccct cggccggggc    240

cgcattcctg ggggccgggc ggtgctcccg cccgcctcga taaaaggctc cggggccggc    300

ggcggcccac gagctacccg gaggagcggg aggcgccaag cggatcccaa ggcccaactc    360

cccgaaccac tcagggtcct gtggacagct cacctagctg ccatggccct gtgggtgttc    420

tttgtgctgc tgaccctgac ctccggaagc cactgcagcc tgccacccag cccacccttc    480

cgcgtcaggc gccacgccga cggcatcttc agcaaggcct accgcaagct cctgggccag    540

ctgagcgcac gcaactacct gcacagcctg atggccaagc gcgtgggcag cggactggga    600

gacgaggccg agcccctgag ctgataagct tatcggggtg gcatccctgt gacccctccc    660

cagtgcctct cctggccctg gaagttgcca ctccagtgcc caccagcctt gtcctaataa    720

aattaagttg catcattttg tctgactagg tgtccttcta taatattatg gggtggaggg    780

gggtggtatg gagcaagggg caagttggga agacaacctg tagggctcga gggggggccc    840

ggtaccagct tttgttccct ttagtgaggg ttaatttcga gcttggtctt ccgcttcctc    900

gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa    960

ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa   1020

aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct   1080

ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac   1140

aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc   1200

gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc   1260

tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg   1320

tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga   1380

gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag   1440

cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta   1500

cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag   1560

agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgttta   1620

caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac   1680

ggggtctgac gctcagctag cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg   1740

cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg   1800

ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca   1860

cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc   1920

aagcaggcat cgccatgagt cacgacgaga tcctcgccgt cgggcatgcg cgccttgagc   1980

ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg   2040

acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg   2100

aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat   2160

actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat   2220

agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc   2280

gtcgtggcca gccacgatag ccgcgctgcc tcgtcctgca gttcattcag ggcaccggac   2340

aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca   2400

tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg   2460

gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc   2520

tcttgatcag atcttgatcc cctgcgccat cagatccttg gcggcaagaa agccatccag   2580

tttactttgc agggcttccc aaccttacca gagggcgccc cagctggcaa ttccggttcg   2640

cttgctgtcc ataaaaccgc ccagtctagc aactgttggg aagggcgatc gtgtaatacg   2700

actcactata gggcgaattg gagct                                         2725


<210>  33
<211>  5
<212>  DNA
<213>  artificial sequence

<220>
<223>  This is the codon optimized Horse-GHRH expression plasmid.

<400>  33
aaaaa                                                                  5


<210>  34
<211>  5
<212>  DNA
<213>  artificial sequence

<220>
<223>  This is the codon optimized human-GHRH expression plasmid.

<400>  34
ccaccgcggt ggcggccgtc cgccctcggc accatcctca cgacacccaa atatggcgac     60

gggtgaggaa tggtggggag ttatttttag agcggtgagg aaggtgggca ggcagcaggt    120

gttggcgctc taaaaataac tcccgggagt tatttttaga gcggaggaat ggtggacacc    180

caaatatggc gacggttcct cacccgtcgc catatttggg tgtccgccct cggccggggc    240

cgcattcctg ggggccgggc ggtgctcccg cccgcctcga taaaaggctc cggggccggc    300

ggcggcccac gagctacccg gaggagcggg aggcgccaag cggatcccaa ggcccaactc    360

cccgaaccac tcagggtcct gtggacagct cacctagctg ccatggtgct ctgggtgttc    420

ttctttgtga tcctcaccct cagcaacagc tcccactgct ccccacctcc ccctttgacc    480

ctcaggatgc ggcggtatgc agatgccatc ttcaccaaca gctaccggaa ggtgctgggc    540

cagctgtccg cccgcaagct gctgcaggac atcatgagca ggcagcaggg agagagcaac    600

caagagcgag gagcataatg aaagcttatc ggggtggcat ccctgtgacc cctccccagt    660

gcctctcctg gccctggaag ttgccactcc agtgcccacc agccttgtcc taataaaatt    720

aagttgcatc attttgtctg actaggtgtc cttctataat attatggggt ggaggggggt    780

ggtatggagc aaggggcaag ttgggaagac aacctgtagg gctcgagggg gggcccggta    840

ccatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt    900

ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt    960

ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc   1020

gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa   1080

gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct   1140

ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta   1200

actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg   1260

gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc   1320

ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta   1380

ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg   1440

gtttttttgt ttacaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt   1500

tgatcttttc tacggggtct gacgctcagc tagcgctcag aagaactcgt caagaaggcg   1560

atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc   1620

agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata   1680

gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac   1740

catgatattc ggcaagcagg catcgccatg agtcacgacg agatcctcgc cgtcgggcat   1800

gcgcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag   1860

atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt   1920

cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc   1980

agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg   2040

cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc   2100

gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct gcagttcatt   2160

cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg   2220

gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct   2280

ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga   2340

tcctcatcct gtctcttgat cagatcttga tcccctgcgc catcagatcc ttggcggcaa   2400

gaaagccatc cagtttactt tgcagggctt cccaacctta ccagagggcg ccccagctgg   2460

caattccggt tcgcttgctg tccataaaac cgcccagtct gagct			 2505


<210>  35
<211>  3534
<212>  DNA
<213>  artificial sequence

<220>
<223>  Plasmid vector having a porcine wt-GHRH sequence.

<400>  35
ccaccgcggt ggcggccgtc cgccctcggc accatcctca cgacacccaa atatggcgac     60

gggtgaggaa tggtggggag ttatttttag agcggtgagg aaggtgggca ggcagcaggt    120

gttggcgctc taaaaataac tcccgggagt tatttttaga gcggaggaat ggtggacacc    180

caaatatggc gacggttcct cacccgtcgc catatttggg tgtccgccct cggccggggc    240

cgcattcctg ggggccgggc ggtgctcccg cccgcctcga taaaaggctc cggggccggc    300

ggcggcccac gagctacccg gaggagcggg aggcgccaag ctctagaact agtggatccc    360

aaggcccaac tccccgaacc actcagggtc ctgtggacag ctcacctagc tgccatggtg    420

ctctgggtgt tcttctttgt gatcctcacc ctcagcaaca gctcccactg ctccccacct    480

ccccctttga ccctcaggat gcggcggtat gcagatgcca tcttcaccaa cagctaccgg    540

aaggtgctgg gccagctgtc cgcccgcaag ctgctccagg acatcatgag caggcagcag    600

ggagagagga accaagagca aggagcataa tgactgcagg aattcgatat caagcttatc    660

ggggtggcat ccctgtgacc cctccccagt gcctctcctg gccctggaag ttgccactcc    720

agtgcccacc agccttgtcc taataaaatt aagttgcatc attttgtctg actaggtgtc    780

cttctataat attatggggt ggaggggggt ggtatggagc aaggggcaag ttgggaagac    840

aacctgtagg gcctgcgggg tctattggga accaagctgg agtgcagtgg cacaatcttg    900

gctcactgca atctccgcct cctgggttca agcgattctc ctgcctcagc ctcccgagtt    960

gttgggattc caggcatgca tgaccaggct cagctaattt ttgttttttt ggtagagacg   1020

gggtttcacc atattggcca ggctggtctc caactcctaa tctcaggtga tctacccacc   1080

ttggcctccc aaattgctgg gattacaggc gtgaaccact gctcccttcc ctgtccttct   1140

gattttaaaa taactatacc agcaggagga cgtccagaca cagcataggc tacctggcca   1200

tgcccaaccg gtgggacatt tgagttgctt gcttggcact gtcctctcat gcgttgggtc   1260

cactcagtag atgcctgttg aattcgatac cgtcgacctc gagggggggc ccggtaccag   1320

cttttgttcc ctttagtgag ggttaatttc gagcttggcg taatcatggt catagctgtt   1380

tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa   1440

gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact   1500

gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc   1560

ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg   1620

ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc   1680

cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag   1740

gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca   1800

tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca   1860

ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg   1920

atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag   1980

gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt   2040

tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca   2100

cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg   2160

cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt   2220

tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc   2280

cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg   2340

cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagaa   2400

gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta   2460

aagcacgagg aagcggtcag cccattcgcc gccaagctct tcagcaatat cacgggtagc   2520

caacgctatg tcctgatagc ggtccgccac acccagccgg ccacagtcga tgaatccaga   2580

aaagcggcca ttttccacca tgatattcgg caagcaggca tcgccatggg tcacgacgag   2640

atcctcgccg tcgggcatgc gcgccttgag cctggcgaac agttcggctg gcgcgagccc   2700

ctgatgctct tcgtccagat catcctgatc gacaagaccg gcttccatcc gagtacgtgc   2760

tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag gtagccggat caagcgtatg   2820

cagccgccgc attgcatcag ccatgatgga tactttctcg gcaggagcaa ggtgagatga   2880

caggagatcc tgccccggca cttcgcccaa tagcagccag tcccttcccg cttcagtgac   2940

aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc   3000

ctcgtcctgc agttcattca gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg   3060

cccctgcgct gacagccgga acacggcggc atcagagcag ccgattgtct gttgtgccca   3120

gtcatagccg aatagcctct ccacccaagc ggccggagaa cctgcgtgca atccatcttg   3180

ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca gatcttgatc ccctgcgcca   3240

tcagatcctt ggcggcaaga aagccatcca gtttactttg cagggcttcc caaccttacc   3300

agagggcgcc ccagctggca attccggttc gcttgctgtc cataaaaccg cccagtctag   3360

caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg   3420

gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg   3480

taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg agct		 3534


<210>  36
<211>  2506
<212>  DNA
<213>  artificial sequence

<220>
<223>  This is the codon optimized mouse GHRH expression plasmid.

<400>  36
ccaccgcggt ggcggccgtc cgccctcggc accatcctca cgacacccaa atatggcgac     60

gggtgaggaa tggtggggag ttatttttag agcggtgagg aaggtgggca ggcagcaggt    120

gttggcgctc taaaaataac tcccgggagt tatttttaga gcggaggaat ggtggacacc    180

caaatatggc gacggttcct cacccgtcgc catatttggg tgtccgccct cggccggggc    240

cgcattcctg ggggccgggc ggtgctcccg cccgcctcga taaaaggctc cggggccggc    300

ggcggcccac gagctacccg gaggagcggg aggcgccaag cggatcccaa ggcccaactc    360

cccgaaccac tcagggtcct gtggacagct cacctagctg ccatggtgct ctgggtgctc    420

tttgtgatcc tcatcctcac cagcggcagc cactgcagcc tgcctcccag ccctcccttc    480

aggatgcaga ggcacgtgga cgccatcttc accaccaact acaggaagct gctgagccag    540

ctgtacgcca ggaaggtgat ccaggacatc atgaacaagc agggcgagag gatccaggag    600

cagagggcca ggctgagctg ataagcttat cggggtggca tccctgtgac ccctccccag    660

tgcctctcct ggccctggaa gttgccactc cagtgcccac cagccttgtc ctaataaaat    720

taagttgcat cattttgtct gactaggtgt ccttctataa tattatgggg tggagggggg    780

tggtatggag caaggggcaa gttgggaaga caacctgtag ggctcgaggg ggggcccggt    840

accatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg    900

tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg    960

tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg   1020

cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga   1080

agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc   1140

tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt   1200

aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact   1260

ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg   1320

cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt   1380

accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt   1440

ggtttttttg tttacaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct   1500

ttgatctttt ctacggggtc tgacgctcag ctagcgctca gaagaactcg tcaagaaggc   1560

gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg aggaagcggt   1620

cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct atgtcctgat   1680

agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg ccattttcca   1740

ccatgatatt cggcaagcag gcatcgccat gagtcacgac gagatcctcg ccgtcgggca   1800

tgcgcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca   1860

gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt   1920

tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc cgcattgcat   1980

cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga tcctgccccg   2040

gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg agcacagctg   2100

cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtcc tgcagttcat   2160

tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc   2220

ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag ccgaatagcc   2280

tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg   2340

atcctcatcc tgtctcttga tcagatcttg atcccctgcg ccatcagatc cttggcggca   2400

agaaagccat ccagtttact ttgcagggct tcccaacctt accagagggc gccccagctg   2460

gcaattccgg ttcgcttgct gtccataaaa ccgcccagtc tgagct			 2506


<210>  37
<211>  2725
<212>  DNA
<213>  artificial sequence

<220>
<223>  This is the codon optimized rat GHRH expression plasmid.

<400>  37
ccaccgcggt ggcggccgtc cgccctcggc accatcctca cgacacccaa atatggcgac     60

gggtgaggaa tggtggggag ttatttttag agcggtgagg aaggtgggca ggcagcaggt    120

gttggcgctc taaaaataac tcccgggagt tatttttaga gcggaggaat ggtggacacc    180

caaatatggc gacggttcct cacccgtcgc catatttggg tgtccgccct cggccggggc    240

cgcattcctg ggggccgggc ggtgctcccg cccgcctcga taaaaggctc cggggccggc    300

ggcggcccac gagctacccg gaggagcggg aggcgccaag cggatcccaa ggcccaactc    360

cccgaaccac tcagggtcct gtggacagct cacctagctg ccatggccct gtgggtgttc    420

ttcgtgctgc tgaccctgac cagcggaagc cactgcagcc tgcctcccag ccctcccttc    480

agggtgcgcc ggcacgccga cgccatcttc accagcagct acaggaggat cctgggccag    540

ctgtacgcta ggaagctcct gcacgagatc atgaacaggc agcagggcga gaggaaccag    600

gagcagagga gcaggttcaa ctgataagct tatcggggtg gcatccctgt gacccctccc    660

cagtgcctct cctggccctg gaagttgcca ctccagtgcc caccagcctt gtcctaataa    720

aattaagttg catcattttg tctgactagg tgtccttcta taatattatg gggtggaggg    780

gggtggtatg gagcaagggg caagttggga agacaacctg tagggctcga gggggggccc    840

ggtaccagct tttgttccct ttagtgaggg ttaatttcga gcttggtctt ccgcttcctc    900

gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa    960

ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa   1020

aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct   1080

ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac   1140

aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc   1200

gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc   1260

tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg   1320

tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga   1380

gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag   1440

cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta   1500

cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag   1560

agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgttta   1620

caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac   1680

ggggtctgac gctcagctag cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg   1740

cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg   1800

ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca   1860

cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc   1920

aagcaggcat cgccatgagt cacgacgaga tcctcgccgt cgggcatgcg cgccttgagc   1980

ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg   2040

acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg   2100

aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat   2160

actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat   2220

agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc   2280

gtcgtggcca gccacgatag ccgcgctgcc tcgtcctgca gttcattcag ggcaccggac   2340

aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca   2400

tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg   2460

gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc   2520

tcttgatcag atcttgatcc cctgcgccat cagatccttg gcggcaagaa agccatccag   2580

tttactttgc agggcttccc aaccttacca gagggcgccc cagctggcaa ttccggttcg   2640

cttgctgtcc ataaaaccgc ccagtctagc aactgttggg aagggcgatc gtgtaatacg   2700

actcactata gggcgaattg gagct						 2725

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4284223v.1

