Sequenzprotokoll

<110>  Westflische Wilhelms-Universitt Mnster
 
<120>  Mikroorganimus und Verfahren zur Herstellung von 
       2-Methylzitronensure

<130>  P35211

<160>  9     

<170>  PatentIn version 3.3

<210>  1
<211>  1128
<212>  DNA
<213>  Pseudomonas putida prpC-Gen CDS


<220>
<221>  CDS
<222>  (1)..(1128)

<400>  1
atg gcc gaa gca aaa gta ctc agt ggc gca ggc ctg cgt ggc cag gtg       48
Met Ala Glu Ala Lys Val Leu Ser Gly Ala Gly Leu Arg Gly Gln Val         
1               5                   10                  15              

gcc ggc cag act gca ctg tcg acc gtg ggc cag gcc ggt gcc ggg ctg       96
Ala Gly Gln Thr Ala Leu Ser Thr Val Gly Gln Ala Gly Ala Gly Leu         
            20                  25                  30                  

acc tac cgt ggt tat gat gtg cgt gac ctg gcc gcc ggt gcc gag ttc      144
Thr Tyr Arg Gly Tyr Asp Val Arg Asp Leu Ala Ala Gly Ala Glu Phe         
        35                  40                  45                      

gaa gaa gtc gcc tac ctg ctg ctg tac ggc gag ctg cca acc cag gct      192
Glu Glu Val Ala Tyr Leu Leu Leu Tyr Gly Glu Leu Pro Thr Gln Ala         
    50                  55                  60                          

gaa ctg gcc gac tac aag cgc aag ctc aag ggc ctg cgt gac ctg ccg      240
Glu Leu Ala Asp Tyr Lys Arg Lys Leu Lys Gly Leu Arg Asp Leu Pro         
65                  70                  75                  80          

caa gcc ttg aag gaa gtg ctt gag cgc atc ccg cgc gat gcc cac ccg      288
Gln Ala Leu Lys Glu Val Leu Glu Arg Ile Pro Arg Asp Ala His Pro         
                85                  90                  95              

atg gac gtc atg cgc acc ggt tgc tcg gta ctg ggc acc ctc gaa cca      336
Met Asp Val Met Arg Thr Gly Cys Ser Val Leu Gly Thr Leu Glu Pro         
            100                 105                 110                 

gaa ctg acc ttc gaa gcc cag cgc gac aag acc gac cgc ctg ctg gcg      384
Glu Leu Thr Phe Glu Ala Gln Arg Asp Lys Thr Asp Arg Leu Leu Ala         
        115                 120                 125                     

ctg ttc ccg gcc gtg atg tgc tac tgg tac cgc ttc acc cac cat ggc      432
Leu Phe Pro Ala Val Met Cys Tyr Trp Tyr Arg Phe Thr His His Gly         
    130                 135                 140                         

gta cgc atc gac tgc acc act gat gaa gac acc ttg ggt ggc cac ttc      480
Val Arg Ile Asp Cys Thr Thr Asp Glu Asp Thr Leu Gly Gly His Phe         
145                 150                 155                 160         

ctg cac ctg ctg cac ggc aag aag ccg agc gag ctg cac gtc aag gtc      528
Leu His Leu Leu His Gly Lys Lys Pro Ser Glu Leu His Val Lys Val         
                165                 170                 175             

atg aac gtg tcg ctg att ctc tac gct gaa cac gaa ttc aac gcc tcg      576
Met Asn Val Ser Leu Ile Leu Tyr Ala Glu His Glu Phe Asn Ala Ser         
            180                 185                 190                 

acc ttc act gcc cgc gtc tgc gcc tcg acc ctg tct gac ttg tac tcc      624
Thr Phe Thr Ala Arg Val Cys Ala Ser Thr Leu Ser Asp Leu Tyr Ser         
        195                 200                 205                     

tgc gtt acc gca gcc atc ggc tcg ctg cgt ggc ccg ctg cac ggc ggt      672
Cys Val Thr Ala Ala Ile Gly Ser Leu Arg Gly Pro Leu His Gly Gly         
    210                 215                 220                         

gcc aac gag gct gcg atg gaa ctg atc gaa cgc ttc cag agc ccg cag      720
Ala Asn Glu Ala Ala Met Glu Leu Ile Glu Arg Phe Gln Ser Pro Gln         
225                 230                 235                 240         

gaa gcc acc gcc gaa ctg ctg cgc atg ctc gag cgc aaa gac aag atc      768
Glu Ala Thr Ala Glu Leu Leu Arg Met Leu Glu Arg Lys Asp Lys Ile         
                245                 250                 255             

atg ggc ttt ggc cat gcc atc tac aaa gag tcc gac ccg cgc aac gag      816
Met Gly Phe Gly His Ala Ile Tyr Lys Glu Ser Asp Pro Arg Asn Glu         
            260                 265                 270                 

gtg atc aag ggc tgg tcg aag cag ctg gct gac gaa gtg ggt gac aag      864
Val Ile Lys Gly Trp Ser Lys Gln Leu Ala Asp Glu Val Gly Asp Lys         
        275                 280                 285                     

gtg ctg tac ccg gtg tcc gaa gcc atc gac aag acc atg tgg gag cag      912
Val Leu Tyr Pro Val Ser Glu Ala Ile Asp Lys Thr Met Trp Glu Gln         
    290                 295                 300                         

aag cgc ctg ttc ccc aac gcc gac ttc tac cat gcc tcg gcg tac cac      960
Lys Arg Leu Phe Pro Asn Ala Asp Phe Tyr His Ala Ser Ala Tyr His         
305                 310                 315                 320         

ttc atg ggc att ccg acc aag ctg ttc acc ccg atc ttc gtc tgc tcg     1008
Phe Met Gly Ile Pro Thr Lys Leu Phe Thr Pro Ile Phe Val Cys Ser         
                325                 330                 335             

cgg ctg acc ggc tgg gcg gcg cac gtc ttc gag cag cgc gcc aac aac     1056
Arg Leu Thr Gly Trp Ala Ala His Val Phe Glu Gln Arg Ala Asn Asn         
            340                 345                 350                 

cgc atc atc cgc ccg agc gcc gag tat gtc ggc gtc gaa cag cgc cag     1104
Arg Ile Ile Arg Pro Ser Ala Glu Tyr Val Gly Val Glu Gln Arg Gln         
        355                 360                 365                     

ttc gtg ccg atc gag cag cgc tga                                     1128
Phe Val Pro Ile Glu Gln Arg                                             
    370                 375                                             


<210>  2
<211>  375
<212>  PRT
<213>  Pseudomonas putida prpC-Gen CDS

<400>  2

Met Ala Glu Ala Lys Val Leu Ser Gly Ala Gly Leu Arg Gly Gln Val 
1               5                   10                  15      


Ala Gly Gln Thr Ala Leu Ser Thr Val Gly Gln Ala Gly Ala Gly Leu 
            20                  25                  30          


Thr Tyr Arg Gly Tyr Asp Val Arg Asp Leu Ala Ala Gly Ala Glu Phe 
        35                  40                  45              


Glu Glu Val Ala Tyr Leu Leu Leu Tyr Gly Glu Leu Pro Thr Gln Ala 
    50                  55                  60                  


Glu Leu Ala Asp Tyr Lys Arg Lys Leu Lys Gly Leu Arg Asp Leu Pro 
65                  70                  75                  80  


Gln Ala Leu Lys Glu Val Leu Glu Arg Ile Pro Arg Asp Ala His Pro 
                85                  90                  95      


Met Asp Val Met Arg Thr Gly Cys Ser Val Leu Gly Thr Leu Glu Pro 
            100                 105                 110         


Glu Leu Thr Phe Glu Ala Gln Arg Asp Lys Thr Asp Arg Leu Leu Ala 
        115                 120                 125             


Leu Phe Pro Ala Val Met Cys Tyr Trp Tyr Arg Phe Thr His His Gly 
    130                 135                 140                 


Val Arg Ile Asp Cys Thr Thr Asp Glu Asp Thr Leu Gly Gly His Phe 
145                 150                 155                 160 


Leu His Leu Leu His Gly Lys Lys Pro Ser Glu Leu His Val Lys Val 
                165                 170                 175     


Met Asn Val Ser Leu Ile Leu Tyr Ala Glu His Glu Phe Asn Ala Ser 
            180                 185                 190         


Thr Phe Thr Ala Arg Val Cys Ala Ser Thr Leu Ser Asp Leu Tyr Ser 
        195                 200                 205             


Cys Val Thr Ala Ala Ile Gly Ser Leu Arg Gly Pro Leu His Gly Gly 
    210                 215                 220                 


Ala Asn Glu Ala Ala Met Glu Leu Ile Glu Arg Phe Gln Ser Pro Gln 
225                 230                 235                 240 


Glu Ala Thr Ala Glu Leu Leu Arg Met Leu Glu Arg Lys Asp Lys Ile 
                245                 250                 255     


Met Gly Phe Gly His Ala Ile Tyr Lys Glu Ser Asp Pro Arg Asn Glu 
            260                 265                 270         


Val Ile Lys Gly Trp Ser Lys Gln Leu Ala Asp Glu Val Gly Asp Lys 
        275                 280                 285             


Val Leu Tyr Pro Val Ser Glu Ala Ile Asp Lys Thr Met Trp Glu Gln 
    290                 295                 300                 


Lys Arg Leu Phe Pro Asn Ala Asp Phe Tyr His Ala Ser Ala Tyr His 
305                 310                 315                 320 


Phe Met Gly Ile Pro Thr Lys Leu Phe Thr Pro Ile Phe Val Cys Ser 
                325                 330                 335     


Arg Leu Thr Gly Trp Ala Ala His Val Phe Glu Gln Arg Ala Asn Asn 
            340                 345                 350         


Arg Ile Ile Arg Pro Ser Ala Glu Tyr Val Gly Val Glu Gln Arg Gln 
        355                 360                 365             


Phe Val Pro Ile Glu Gln Arg 
    370                 375 


<210>  3
<211>  2610
<212>  DNA
<213>  Ralstonia eutropha acnM-Gen CDS


<220>
<221>  CDS
<222>  (1)..(2610)

<400>  3
atg aat tct gca aac cgc aaa ccg cta ccg ggc acc aag ctg gat tac       48
Met Asn Ser Ala Asn Arg Lys Pro Leu Pro Gly Thr Lys Leu Asp Tyr         
1               5                   10                  15              

ttt gac gcg cgc gcc gcg gtc gag gca atc cag ccg ggt gcc tac gac       96
Phe Asp Ala Arg Ala Ala Val Glu Ala Ile Gln Pro Gly Ala Tyr Asp         
            20                  25                  30                  

aag ctg ccg tac acg tcg cgc gtg ctg gca gaa aac ctg gtg cgc cgc      144
Lys Leu Pro Tyr Thr Ser Arg Val Leu Ala Glu Asn Leu Val Arg Arg         
        35                  40                  45                      

tgc gat ccc gcc acg ctg acg gat tcg ctc ctg cag ctg gtc ggg cgc      192
Cys Asp Pro Ala Thr Leu Thr Asp Ser Leu Leu Gln Leu Val Gly Arg         
    50                  55                  60                          

aag cgc gac ctc gat ttc ccg tgg ttc ccg gcg cgc gtg gtg tgc cat      240
Lys Arg Asp Leu Asp Phe Pro Trp Phe Pro Ala Arg Val Val Cys His         
65                  70                  75                  80          

gac atc ctg ggc cag acc gcg ctg gtg gac ctg gcc ggc ctg cgc gac      288
Asp Ile Leu Gly Gln Thr Ala Leu Val Asp Leu Ala Gly Leu Arg Asp         
                85                  90                  95              

gcc atc gcc gac cag ggc ggc gac ccc gcc aag gtc aac ccg gta gtg      336
Ala Ile Ala Asp Gln Gly Gly Asp Pro Ala Lys Val Asn Pro Val Val         
            100                 105                 110                 

ccg gtg cag ctg atc gtc gat cat tcg ctg gcg gtg gag tgc ggc ggc      384
Pro Val Gln Leu Ile Val Asp His Ser Leu Ala Val Glu Cys Gly Gly         
        115                 120                 125                     

ttc gac ccc gat gcg ttc gcc aag aac cgc gcc atc gaa gac cgc cgc      432
Phe Asp Pro Asp Ala Phe Ala Lys Asn Arg Ala Ile Glu Asp Arg Arg         
    130                 135                 140                         

aac gaa gac cgc ttc cac ttc atc gac tgg acc aag cag gcg ttc aag      480
Asn Glu Asp Arg Phe His Phe Ile Asp Trp Thr Lys Gln Ala Phe Lys         
145                 150                 155                 160         

aac gtc gac gtg atc ccg ccg ggc aac ggc atc atg cac cag atc aac      528
Asn Val Asp Val Ile Pro Pro Gly Asn Gly Ile Met His Gln Ile Asn         
                165                 170                 175             

ctg gag aag atg tcg ccg gtg atc cac gcc gac aac ggc gtg gcc tac      576
Leu Glu Lys Met Ser Pro Val Ile His Ala Asp Asn Gly Val Ala Tyr         
            180                 185                 190                 

ccc gat acc tgc gtc ggc acc gac agc cac acc ccg cat gtg gat gcg      624
Pro Asp Thr Cys Val Gly Thr Asp Ser His Thr Pro His Val Asp Ala         
        195                 200                 205                     

ctc ggc gtg atc gcc atc ggc gtg ggc ggc ctg gaa gcc gag aac gtg      672
Leu Gly Val Ile Ala Ile Gly Val Gly Gly Leu Glu Ala Glu Asn Val         
    210                 215                 220                         

atg ctc ggc cgc gcc tcg tgg atg cgc ctg ccg gat atc gtc ggc gtg      720
Met Leu Gly Arg Ala Ser Trp Met Arg Leu Pro Asp Ile Val Gly Val         
225                 230                 235                 240         

gag ctg acc gga aag cgc cag ccc ggc atc acc gcc acc gac atc gtg      768
Glu Leu Thr Gly Lys Arg Gln Pro Gly Ile Thr Ala Thr Asp Ile Val         
                245                 250                 255             

ctg gcc ctg act gaa ttc ctg cgc aag gaa aag gtg gtc ggc gcc tat      816
Leu Ala Leu Thr Glu Phe Leu Arg Lys Glu Lys Val Val Gly Ala Tyr         
            260                 265                 270                 

ctg gag ttc cgt ggc gaa ggc gcc tcc agc ctg acg ctc ggc gac cgc      864
Leu Glu Phe Arg Gly Glu Gly Ala Ser Ser Leu Thr Leu Gly Asp Arg         
        275                 280                 285                     

gcg act att tcc aac atg gcc ccg gaa tat ggc gcc act gcc gcg atg      912
Ala Thr Ile Ser Asn Met Ala Pro Glu Tyr Gly Ala Thr Ala Ala Met         
    290                 295                 300                         

ttc ttc atc gac gag cag acc att gac tac ctg cgc ctg acc ggc cgc      960
Phe Phe Ile Asp Glu Gln Thr Ile Asp Tyr Leu Arg Leu Thr Gly Arg         
305                 310                 315                 320         

acc gac gag cag ctc aag ctg gtc gaa acg tac gcc agg act gcc ggc     1008
Thr Asp Glu Gln Leu Lys Leu Val Glu Thr Tyr Ala Arg Thr Ala Gly         
                325                 330                 335             

ctg tgg gcg gat tcg ctg aag aac gcc gaa tac gag cgc gtg ctg aag     1056
Leu Trp Ala Asp Ser Leu Lys Asn Ala Glu Tyr Glu Arg Val Leu Lys         
            340                 345                 350                 

ttc gac ctg tcg agc gtg gtg cgc aat atg gcc ggc ccg tcc aac ccg     1104
Phe Asp Leu Ser Ser Val Val Arg Asn Met Ala Gly Pro Ser Asn Pro         
        355                 360                 365                     

cac aag cgc ctg ccg acc tcc gcg ctg gcc gag cgc ggc atc gcc gtc     1152
His Lys Arg Leu Pro Thr Ser Ala Leu Ala Glu Arg Gly Ile Ala Val         
    370                 375                 380                         

gac ctg gac aag gcc agc gcg cag gaa gcc gaa ggc ctg atg ccg gac     1200
Asp Leu Asp Lys Ala Ser Ala Gln Glu Ala Glu Gly Leu Met Pro Asp         
385                 390                 395                 400         

ggc gcg gtc atc atc gcc gcc atc acc agc tgc acc aac acc agc aac     1248
Gly Ala Val Ile Ile Ala Ala Ile Thr Ser Cys Thr Asn Thr Ser Asn         
                405                 410                 415             

ccg cgc aac gtg atc gct gcc gcg ctg ctg gcg cgc aac gcc aat gcg     1296
Pro Arg Asn Val Ile Ala Ala Ala Leu Leu Ala Arg Asn Ala Asn Ala         
            420                 425                 430                 

cgc ggc ctg gcg cgc aag ccg tgg gtc aag tcg tcg ctg gcc ccg gga     1344
Arg Gly Leu Ala Arg Lys Pro Trp Val Lys Ser Ser Leu Ala Pro Gly         
        435                 440                 445                     

tcc aag gca gtc gag ctg tac ctg gaa gaa gcg aat ctg ctg ccc gac     1392
Ser Lys Ala Val Glu Leu Tyr Leu Glu Glu Ala Asn Leu Leu Pro Asp         
    450                 455                 460                         

ctc gag aag ctt ggc ttc ggc atc gtc gcc ttt gcc tgc acc acc tgc     1440
Leu Glu Lys Leu Gly Phe Gly Ile Val Ala Phe Ala Cys Thr Thr Cys         
465                 470                 475                 480         

aac ggc atg tcc gga gcg ctg gac ccg aag atc cag caa gag atc atc     1488
Asn Gly Met Ser Gly Ala Leu Asp Pro Lys Ile Gln Gln Glu Ile Ile         
                485                 490                 495             

gac cgc gac ctg tac gcg acg gca gtg ctg tcc ggc aac cgc aac ttc     1536
Asp Arg Asp Leu Tyr Ala Thr Ala Val Leu Ser Gly Asn Arg Asn Phe         
            500                 505                 510                 

gat ggc cgc atc cac ccg tac gcc aag cag gcc ttc ctg gcc tcg ccg     1584
Asp Gly Arg Ile His Pro Tyr Ala Lys Gln Ala Phe Leu Ala Ser Pro         
        515                 520                 525                     

ccg ctg gtg gtg gcc tat gcc atc gcc ggc acc atc cgc ttc gat atc     1632
Pro Leu Val Val Ala Tyr Ala Ile Ala Gly Thr Ile Arg Phe Asp Ile         
    530                 535                 540                         

gaa aag gac gtg ctg ggc acg gac cag gac ggc aag ccg gtc tac ctg     1680
Glu Lys Asp Val Leu Gly Thr Asp Gln Asp Gly Lys Pro Val Tyr Leu         
545                 550                 555                 560         

aag gac atc tgg ccg agc gac gaa gag atc gac gcg atc gtg gcg aag     1728
Lys Asp Ile Trp Pro Ser Asp Glu Glu Ile Asp Ala Ile Val Ala Lys         
                565                 570                 575             

agc gtc aag ccc gaa cag ttc cgc aag gtc tac gag ccg atg ttc gcc     1776
Ser Val Lys Pro Glu Gln Phe Arg Lys Val Tyr Glu Pro Met Phe Ala         
            580                 585                 590                 

att acc gcg gcc agc ggc gaa tcg gtc agt ccc ctg tac gac tgg cgt     1824
Ile Thr Ala Ala Ser Gly Glu Ser Val Ser Pro Leu Tyr Asp Trp Arg         
        595                 600                 605                     

ccg cag agc acc tac atc cgc cgc ccg ccc tac tgg gaa ggc gcg ctg     1872
Pro Gln Ser Thr Tyr Ile Arg Arg Pro Pro Tyr Trp Glu Gly Ala Leu         
    610                 615                 620                         

gcc ggc gaa cgc acg ctc aag gcg ctg cgt ccg ctg gcg gtg ctg ggc     1920
Ala Gly Glu Arg Thr Leu Lys Ala Leu Arg Pro Leu Ala Val Leu Gly         
625                 630                 635                 640         

gac aac atc acc acc gac cac ctg tcg ccg tcc aac gcc atc atg ctc     1968
Asp Asn Ile Thr Thr Asp His Leu Ser Pro Ser Asn Ala Ile Met Leu         
                645                 650                 655             

aac agc gcc gcc ggg gaa tac ctg gcc agg atg ggc ttg ccg gaa gag     2016
Asn Ser Ala Ala Gly Glu Tyr Leu Ala Arg Met Gly Leu Pro Glu Glu         
            660                 665                 670                 

gac ttc aac tcg tac gcc acc cac cgc ggc gac cac ctg acc gcg cag     2064
Asp Phe Asn Ser Tyr Ala Thr His Arg Gly Asp His Leu Thr Ala Gln         
        675                 680                 685                     

cgc gcc acc ttt gcc aac ccg acc ctg atc aat gaa atg gct gtg gtc     2112
Arg Ala Thr Phe Ala Asn Pro Thr Leu Ile Asn Glu Met Ala Val Val         
    690                 695                 700                         

gat ggc cag gtc aag aag ggt tcg ctg gcg cgc atc gag cct gaa ggc     2160
Asp Gly Gln Val Lys Lys Gly Ser Leu Ala Arg Ile Glu Pro Glu Gly         
705                 710                 715                 720         

aag gtc gtg cgc atg tgg gaa gcg atc gag acg tac atg gat cgc aag     2208
Lys Val Val Arg Met Trp Glu Ala Ile Glu Thr Tyr Met Asp Arg Lys         
                725                 730                 735             

cag ccg ctg atc atc atc gcc ggc gcg gac tat ggc cag ggc tcg tcg     2256
Gln Pro Leu Ile Ile Ile Ala Gly Ala Asp Tyr Gly Gln Gly Ser Ser         
            740                 745                 750                 

cgc gac tgg gcc gcc aag ggc gtg cgc ctg gcc ggc gtg gaa gtc atc     2304
Arg Asp Trp Ala Ala Lys Gly Val Arg Leu Ala Gly Val Glu Val Ile         
        755                 760                 765                     

gtc gcc gag ggc ttc gag cgc atc cac cgc acc aac ctg atc ggc atg     2352
Val Ala Glu Gly Phe Glu Arg Ile His Arg Thr Asn Leu Ile Gly Met         
    770                 775                 780                         

ggc gtg ctg ccg ctg gag ttc aag ccg ggc gtg aac cgc ctg acg ctg     2400
Gly Val Leu Pro Leu Glu Phe Lys Pro Gly Val Asn Arg Leu Thr Leu         
785                 790                 795                 800         

ggc ctg gac ggc acc gag acc tat gac gtg atc ggc gag cgc cag ccg     2448
Gly Leu Asp Gly Thr Glu Thr Tyr Asp Val Ile Gly Glu Arg Gln Pro         
                805                 810                 815             

cgc gcc acg ctg aca ctg gtg gtg aac cgc aag aac ggc gag cgc gtg     2496
Arg Ala Thr Leu Thr Leu Val Val Asn Arg Lys Asn Gly Glu Arg Val         
            820                 825                 830                 

gaa gtg ccg gtg acc tgc cgc ctc gac agc gac gag gaa gtg tcg atc     2544
Glu Val Pro Val Thr Cys Arg Leu Asp Ser Asp Glu Glu Val Ser Ile         
        835                 840                 845                     

tac gaa gct ggc ggc gtg ctg cac ttc gcg cag gac ttc ctt gag tcg     2592
Tyr Glu Ala Gly Gly Val Leu His Phe Ala Gln Asp Phe Leu Glu Ser         
    850                 855                 860                         

tcg cgg gca act gca taa                                             2610
Ser Arg Ala Thr Ala                                                     
865                                                                     


<210>  4
<211>  869
<212>  PRT
<213>  Ralstonia eutropha acnM-Gen CDS

<400>  4

Met Asn Ser Ala Asn Arg Lys Pro Leu Pro Gly Thr Lys Leu Asp Tyr 
1               5                   10                  15      


Phe Asp Ala Arg Ala Ala Val Glu Ala Ile Gln Pro Gly Ala Tyr Asp 
            20                  25                  30          


Lys Leu Pro Tyr Thr Ser Arg Val Leu Ala Glu Asn Leu Val Arg Arg 
        35                  40                  45              


Cys Asp Pro Ala Thr Leu Thr Asp Ser Leu Leu Gln Leu Val Gly Arg 
    50                  55                  60                  


Lys Arg Asp Leu Asp Phe Pro Trp Phe Pro Ala Arg Val Val Cys His 
65                  70                  75                  80  


Asp Ile Leu Gly Gln Thr Ala Leu Val Asp Leu Ala Gly Leu Arg Asp 
                85                  90                  95      


Ala Ile Ala Asp Gln Gly Gly Asp Pro Ala Lys Val Asn Pro Val Val 
            100                 105                 110         


Pro Val Gln Leu Ile Val Asp His Ser Leu Ala Val Glu Cys Gly Gly 
        115                 120                 125             


Phe Asp Pro Asp Ala Phe Ala Lys Asn Arg Ala Ile Glu Asp Arg Arg 
    130                 135                 140                 


Asn Glu Asp Arg Phe His Phe Ile Asp Trp Thr Lys Gln Ala Phe Lys 
145                 150                 155                 160 


Asn Val Asp Val Ile Pro Pro Gly Asn Gly Ile Met His Gln Ile Asn 
                165                 170                 175     


Leu Glu Lys Met Ser Pro Val Ile His Ala Asp Asn Gly Val Ala Tyr 
            180                 185                 190         


Pro Asp Thr Cys Val Gly Thr Asp Ser His Thr Pro His Val Asp Ala 
        195                 200                 205             


Leu Gly Val Ile Ala Ile Gly Val Gly Gly Leu Glu Ala Glu Asn Val 
    210                 215                 220                 


Met Leu Gly Arg Ala Ser Trp Met Arg Leu Pro Asp Ile Val Gly Val 
225                 230                 235                 240 


Glu Leu Thr Gly Lys Arg Gln Pro Gly Ile Thr Ala Thr Asp Ile Val 
                245                 250                 255     


Leu Ala Leu Thr Glu Phe Leu Arg Lys Glu Lys Val Val Gly Ala Tyr 
            260                 265                 270         


Leu Glu Phe Arg Gly Glu Gly Ala Ser Ser Leu Thr Leu Gly Asp Arg 
        275                 280                 285             


Ala Thr Ile Ser Asn Met Ala Pro Glu Tyr Gly Ala Thr Ala Ala Met 
    290                 295                 300                 


Phe Phe Ile Asp Glu Gln Thr Ile Asp Tyr Leu Arg Leu Thr Gly Arg 
305                 310                 315                 320 


Thr Asp Glu Gln Leu Lys Leu Val Glu Thr Tyr Ala Arg Thr Ala Gly 
                325                 330                 335     


Leu Trp Ala Asp Ser Leu Lys Asn Ala Glu Tyr Glu Arg Val Leu Lys 
            340                 345                 350         


Phe Asp Leu Ser Ser Val Val Arg Asn Met Ala Gly Pro Ser Asn Pro 
        355                 360                 365             


His Lys Arg Leu Pro Thr Ser Ala Leu Ala Glu Arg Gly Ile Ala Val 
    370                 375                 380                 


Asp Leu Asp Lys Ala Ser Ala Gln Glu Ala Glu Gly Leu Met Pro Asp 
385                 390                 395                 400 


Gly Ala Val Ile Ile Ala Ala Ile Thr Ser Cys Thr Asn Thr Ser Asn 
                405                 410                 415     


Pro Arg Asn Val Ile Ala Ala Ala Leu Leu Ala Arg Asn Ala Asn Ala 
            420                 425                 430         


Arg Gly Leu Ala Arg Lys Pro Trp Val Lys Ser Ser Leu Ala Pro Gly 
        435                 440                 445             


Ser Lys Ala Val Glu Leu Tyr Leu Glu Glu Ala Asn Leu Leu Pro Asp 
    450                 455                 460                 


Leu Glu Lys Leu Gly Phe Gly Ile Val Ala Phe Ala Cys Thr Thr Cys 
465                 470                 475                 480 


Asn Gly Met Ser Gly Ala Leu Asp Pro Lys Ile Gln Gln Glu Ile Ile 
                485                 490                 495     


Asp Arg Asp Leu Tyr Ala Thr Ala Val Leu Ser Gly Asn Arg Asn Phe 
            500                 505                 510         


Asp Gly Arg Ile His Pro Tyr Ala Lys Gln Ala Phe Leu Ala Ser Pro 
        515                 520                 525             


Pro Leu Val Val Ala Tyr Ala Ile Ala Gly Thr Ile Arg Phe Asp Ile 
    530                 535                 540                 


Glu Lys Asp Val Leu Gly Thr Asp Gln Asp Gly Lys Pro Val Tyr Leu 
545                 550                 555                 560 


Lys Asp Ile Trp Pro Ser Asp Glu Glu Ile Asp Ala Ile Val Ala Lys 
                565                 570                 575     


Ser Val Lys Pro Glu Gln Phe Arg Lys Val Tyr Glu Pro Met Phe Ala 
            580                 585                 590         


Ile Thr Ala Ala Ser Gly Glu Ser Val Ser Pro Leu Tyr Asp Trp Arg 
        595                 600                 605             


Pro Gln Ser Thr Tyr Ile Arg Arg Pro Pro Tyr Trp Glu Gly Ala Leu 
    610                 615                 620                 


Ala Gly Glu Arg Thr Leu Lys Ala Leu Arg Pro Leu Ala Val Leu Gly 
625                 630                 635                 640 


Asp Asn Ile Thr Thr Asp His Leu Ser Pro Ser Asn Ala Ile Met Leu 
                645                 650                 655     


Asn Ser Ala Ala Gly Glu Tyr Leu Ala Arg Met Gly Leu Pro Glu Glu 
            660                 665                 670         


Asp Phe Asn Ser Tyr Ala Thr His Arg Gly Asp His Leu Thr Ala Gln 
        675                 680                 685             


Arg Ala Thr Phe Ala Asn Pro Thr Leu Ile Asn Glu Met Ala Val Val 
    690                 695                 700                 


Asp Gly Gln Val Lys Lys Gly Ser Leu Ala Arg Ile Glu Pro Glu Gly 
705                 710                 715                 720 


Lys Val Val Arg Met Trp Glu Ala Ile Glu Thr Tyr Met Asp Arg Lys 
                725                 730                 735     


Gln Pro Leu Ile Ile Ile Ala Gly Ala Asp Tyr Gly Gln Gly Ser Ser 
            740                 745                 750         


Arg Asp Trp Ala Ala Lys Gly Val Arg Leu Ala Gly Val Glu Val Ile 
        755                 760                 765             


Val Ala Glu Gly Phe Glu Arg Ile His Arg Thr Asn Leu Ile Gly Met 
    770                 775                 780                 


Gly Val Leu Pro Leu Glu Phe Lys Pro Gly Val Asn Arg Leu Thr Leu 
785                 790                 795                 800 


Gly Leu Asp Gly Thr Glu Thr Tyr Asp Val Ile Gly Glu Arg Gln Pro 
                805                 810                 815     


Arg Ala Thr Leu Thr Leu Val Val Asn Arg Lys Asn Gly Glu Arg Val 
            820                 825                 830         


Glu Val Pro Val Thr Cys Arg Leu Asp Ser Asp Glu Glu Val Ser Ile 
        835                 840                 845             


Tyr Glu Ala Gly Gly Val Leu His Phe Ala Gln Asp Phe Leu Glu Ser 
    850                 855                 860                 


Ser Arg Ala Thr Ala 
865                 


<210>  5
<211>  37
<212>  DNA
<213>  artificial

<220>
<223>  acnMReUP (PstI) Oligonukleotid-Primer fr die PCR

<400>  5
aaactgcagc aaggaaaagg tggtcggcgc ctatctg                              37


<210>  6
<211>  34
<212>  DNA
<213>  artificial

<220>
<223>  acnMReRP (XbaI) Oligonukleotid-Primer fr die PCR

<400>  6
aaatctagac aggtggtcgg tggtgatgtt gtcg                                 34


<210>  7
<211>  38
<212>  DNA
<213>  artificial

<220>
<223>  prpCPpUP (HindIII) Oligonukleotid-Primer fr die PCR

<400>  7
aaaaaagctt caagaaagga gaaacaccat ggccgaag                             38


<210>  8
<211>  40
<212>  DNA
<213>  artificial

<220>
<223>  prpCPpRP(ClaI) Oligonukleotid-Primer fr die PCR

<400>  8
aaaatcgata gctattcagc gctgctcgat cggcacgaac                           40


<210>  9
<211>  40
<212>  DNA
<213>  artificial

<220>
<223>  Omega KmUP (SmaI) Oligonukleotid-Primer fr die PCR

<400>  9
aaacccggga cagcaagcga accggaatga ccagctgggg                           40
