                         SEQUENCE LISTING<110>  Prosensa BV<120>  A novel mRNA splice variant of the doublecortin-like kinase gene 
	and its use in diagnosis and therapy of cancers of neuroectodermal origin <130>  <160>  20    <170>  PatentIn version 3.1<210>  1<211>  1283<212>  DNA<213>  unknown<220><223>  cDNA sequence of dcl (mouse)

<220><221>  startcodon<222>  (189)..(191)<223>  

<220><221>  exon2<222>  (169)..(564)<223>  start of exon 2 at 169

<220><221>  exon3<222>  (565)..(911)<223>  start of exon 3 at 565

<220><221>  exon4<222>  (912)..(1011)<223>  start of exon 4 at 912

<220><221>  exon5<222>  (1012)..(1128)<223>  start of exon 5 at 1012

<220><221>  exon7<222>  (1129)..(1223)<223>  start of exon 7 at 1129

<220><221>  exon8<222>  (1224)..(1274)<223>  start of exon 8 at 1224

<220><221>  stopcodon<222>  (1275)..(1277)<223>  

<400>  1
ccacgcgtcc gcggagaacc gcatttcaat gaggaccagc tccagcgcat cagtgcacta     60

gcggtcgcag cttccagacg ctcgtgctcc gcagccccag ccgcgcccag cccggcgagg    120

acagctccag cagccggcca cagacaaccc agcctccacc cgcgaccggt tccataagca    180

agccagccat gtcgttcggc agagatatgg agttggagca ttttgatgag cgggacaagg    240

cgcagaggta cagcaggggg tcccgtgtga atggcctgcc cagccccaca cacagcgccc    300

actgcagctt ctaccgcacc cgcaccctgc agacactcag ctccgagaag aaagccaaga    360

aggttcgatt ctacagaaat ggtgaccgct acttcaaagg aattgtgtat gccatctccc    420

cagaccgctt cagatctttc gaggccctgc tggctgattt gacccgaact ctctcggata    480

atgtgaattt gccccagggg gtgagaacca tctacaccat cgatggactc aagaagatct    540

ccagcctgga ccagctggtg gaaggtgaaa gctatgtctg cggctccatc gagcccttta    600

agaagctgga gtacaccaag aatgtgaacc ccaactggtc agtgaacgtc aagaccacct    660

cagcctcccg cgcagtgtct tctttggcca ctgccaaggg tgggccttcg gaggttcggg    720

agaataagga tttcattcga cccaagctgg tcaccatcat cagaagtggg gtgaagccac    780

ggaaggctgt cagaatcctg ctgaacaaga agacggctca ctccttcgag caggttctca    840

ctgacattac cgacgctatc aagctggact ccggtgtggt gaagcgcctg tacactctgg    900

atgggaagca ggtgatgtgc cttcaggact tttttggtga cgatgacatt tttattgcat    960

gtggaccaga gaagttccgt taccaggatg atttcttgct agatgaaagt gaatgtcgag   1020

tggtgaaatc aacttcttac accaaaatag catcagcgtc ccgcagaggc acaaccaaga   1080

gcccaggacc ttcccggaga agcaagtccc cagcctccac cagctcagtt aatggaaccc   1140

ctggtagtca gctctctact ccacgctcgg gcaagtcacc aagtccatca cccaccagcc   1200

caggaagcct gcggaagcag agggacctgt accgccccct ctcgtcggat gatttggact   1260

caggagactc agtgtaagaa ttc                                           1283


<210>  2<211>  1310<212>  DNA<213>  unknown<220><223>  cDNA sequence of human dcl

<220><221>  startcodon<222>  (213)..(215)<223>  translation start

<220><221>  stopcodon<222>  (1302)..(1304)<223>  translation stop

<220><221>  exon2<222>  (194)..(588)<223>  start of exon 2 at 194

<220><221>  exon3<222>  (589)..(935)<223>  start of exon 3 at 589

<220><221>  exon4<222>  (936)..(1035)<223>  start of exon 4 at 936

<220><221>  exon5<222>  (1036)..(1152)<223>  start of exon 5 at 1036

<220><221>  exon7<222>  (1153)..(1247)<223>  start of exon 7 at 1153

<220><221>  exon8<222>  (1248)..(1301)<223>  start of exon 8 at 1248

<400>  2
gcacatccct gcactagtgg ccgcaaccga gacgccgcgc tccagcagct gctgccgccc     60

agcccggccc cgccgccgcc ccccagccct gcagccccgc agccccggcc gcgcccagcc    120

cggcgaggac agcaccagga ggcggccccc agcgcggcca caaagacccc cggcggcgtc    180

tctccgcgga ccggtcctac ttgaagtcca tcatgtcctt cggcagagac atggagctgg    240

agcacttcga cgagcgggat aaggcgcaga gatacagccg agggtcgcgg gtgaacggcc    300

tgccgagccc gacgcacagc gcccactgca gcttctaccg cacccgcacg ctgcagacgc    360

tcagctccga gaagaaggcc aagaaagttc gtttctatcg aaacggagat cgatacttca    420

aagggattgt gtatgccatc tccccagacc ggttccgatc ttttgaggcc ctgctggctg    480

atttgacccg aactctgtcg gataacgtga atttgcccca gggagtgaga acaatctaca    540

ccattgatgg gctcaagaag atttccagcc tggaccaact ggtggaagga gagagttatg    600

tatgtggctc catagagccc ttcaagaaac tggagtacac caagaatgtg aaccccaact    660

ggtcggtgaa cgtcaagacc acctcggctt ctcgggcagt gtcttcactg gccactgcca    720

aaggaagccc ttcagaggtg cgagagaata aggatttcat tcggcccaag ctggtcacca    780

tcatcagaag tggcgtgaag ccacggaaag ctgtcaggat tctgctgaac aagaaaacgg    840

ctcattcctt tgagcaggtc ctcaccgata tcaccgatgc catcaagctg gactcgggag    900

tggtgaaacg cctgtacacg ttggatggga aacaggtgat gtgccttcag gacttttttg    960

gtgatgatga catttttatt gcatgtggac cggagaagtt ccgttaccag gatgatttct   1020

tgctagatga aagtgaatgt cgagtggtaa agtccacttc ttacaccaaa atagcttcat   1080

catcccgcag gagcaccacc aagagcccag gaccgtccag gcgtagcaag tcccctgcct   1140

ccaccagctc agttaatgga acccctggta gtcagctctc tactccgcgc tcaggcaagt   1200

cgccaagccc atcacccacc agcccaggaa gcctgcggaa gcagagggac ctgtaccgcc   1260

ccctctcttc ggatgacttg gattcagtag gagactcagt gtaaaagaaa              1310


<210>  3<211>  362<212>  PRT<213>  unknown<220><223>  amino acid sequence of DCL (mouse)

<400>  3

Met Ser Phe Gly Arg Asp Met Glu Leu Glu His Phe Asp Glu Arg Asp 
1               5                   10                  15      


Lys Ala Gln Arg Tyr Ser Arg Gly Ser Arg Val Asn Gly Leu Pro Ser 
            20                  25                  30          


Pro Thr His Ser Ala His Cys Ser Phe Tyr Arg Thr Arg Thr Leu Gln 
        35                  40                  45              


Thr Leu Ser Ser Glu Lys Lys Ala Lys Lys Val Arg Phe Tyr Arg Asn 
    50                  55                  60                  


Gly Asp Arg Tyr Phe Lys Gly Ile Val Tyr Ala Ile Ser Pro Asp Arg 
65                  70                  75                  80  


Phe Arg Ser Phe Glu Ala Leu Leu Ala Asp Leu Thr Arg Thr Leu Ser 
                85                  90                  95      


Asp Asn Val Asn Leu Pro Gln Gly Val Arg Thr Ile Tyr Thr Ile Asp 
            100                 105                 110         


Gly Leu Lys Lys Ile Ser Ser Leu Asp Gln Leu Val Glu Gly Glu Ser 
        115                 120                 125             


Tyr Val Cys Gly Ser Ile Glu Pro Phe Lys Lys Leu Glu Tyr Thr Lys 
    130                 135                 140                 


Asn Val Asn Pro Asn Trp Ser Val Asn Val Lys Thr Thr Ser Ala Ser 
145                 150                 155                 160 


Arg Ala Val Ser Ser Leu Ala Thr Ala Lys Gly Gly Pro Ser Glu Val 
                165                 170                 175     


Arg Glu Asn Lys Asp Phe Ile Arg Pro Lys Leu Val Thr Ile Ile Arg 
            180                 185                 190         


Ser Gly Val Lys Pro Arg Lys Ala Val Arg Ile Leu Leu Asn Lys Lys 
        195                 200                 205             


Thr Ala His Ser Phe Glu Gln Val Leu Thr Asp Ile Thr Asp Ala Ile 
    210                 215                 220                 


Lys Leu Asp Ser Gly Val Val Lys Arg Leu Tyr Thr Leu Asp Gly Lys 
225                 230                 235                 240 


Gln Val Met Cys Leu Gln Asp Phe Phe Gly Asp Asp Asp Ile Phe Ile 
                245                 250                 255     


Ala Cys Gly Pro Glu Lys Phe Arg Tyr Gln Asp Asp Phe Leu Leu Asp 
            260                 265                 270         


Glu Ser Glu Cys Arg Val Val Lys Ser Thr Ser Tyr Thr Lys Ile Ala 
        275                 280                 285             


Ser Ala Ser Arg Arg Gly Thr Thr Lys Ser Pro Gly Pro Ser Arg Arg 
    290                 295                 300                 


Ser Lys Ser Pro Ala Ser Thr Ser Ser Val Asn Gly Thr Pro Gly Ser 
305                 310                 315                 320 


Gln Leu Ser Thr Pro Arg Ser Gly Lys Ser Pro Ser Pro Ser Pro Thr 
                325                 330                 335     


Ser Pro Gly Ser Leu Arg Lys Gln Arg Asp Leu Tyr Arg Pro Leu Ser 
            340                 345                 350         


Ser Asp Asp Leu Asp Ser Gly Asp Ser Val 
        355                 360         


<210>  4<211>  363<212>  PRT<213>  unknown<220><223>  amino acid sequence of DCL (human)

<400>  4

Met Ser Phe Gly Arg Asp Met Glu Leu Glu His Phe Asp Glu Arg Asp 
1               5                   10                  15      


Lys Ala Gln Arg Tyr Ser Arg Gly Ser Arg Val Asn Gly Leu Pro Ser 
            20                  25                  30          


Pro Thr His Ser Ala His Cys Ser Phe Tyr Arg Thr Arg Thr Leu Gln 
        35                  40                  45              


Thr Leu Ser Ser Glu Lys Lys Ala Lys Lys Val Arg Phe Tyr Arg Asn 
    50                  55                  60                  


Gly Asp Arg Tyr Phe Lys Gly Ile Val Tyr Ala Ile Ser Pro Asp Arg 
65                  70                  75                  80  


Phe Arg Ser Phe Glu Ala Leu Leu Ala Asp Leu Thr Arg Thr Leu Ser 
                85                  90                  95      


Asp Asn Val Asn Leu Pro Gln Gly Val Arg Thr Ile Tyr Thr Ile Asp 
            100                 105                 110         


Gly Leu Lys Lys Ile Ser Ser Leu Asp Gln Leu Val Glu Gly Glu Ser 
        115                 120                 125             


Tyr Val Cys Gly Ser Ile Glu Pro Phe Lys Lys Leu Glu Tyr Thr Lys 
    130                 135                 140                 


Asn Val Asn Pro Asn Trp Ser Val Asn Val Lys Thr Thr Ser Ala Ser 
145                 150                 155                 160 


Arg Ala Val Ser Ser Leu Ala Thr Ala Lys Gly Ser Pro Ser Glu Val 
                165                 170                 175     


Arg Glu Asn Lys Asp Phe Ile Arg Pro Lys Leu Val Thr Ile Ile Arg 
            180                 185                 190         


Ser Gly Val Lys Pro Arg Lys Ala Val Arg Ile Leu Leu Asn Lys Lys 
        195                 200                 205             


Thr Ala His Ser Phe Glu Gln Val Leu Thr Asp Ile Thr Asp Ala Ile 
    210                 215                 220                 


Lys Leu Asp Ser Gly Val Val Lys Arg Leu Tyr Thr Leu Asp Gly Lys 
225                 230                 235                 240 


Gln Val Met Cys Leu Gln Asp Phe Phe Gly Asp Asp Asp Ile Phe Ile 
                245                 250                 255     


Ala Cys Gly Pro Glu Lys Phe Arg Tyr Gln Asp Asp Phe Leu Leu Asp 
            260                 265                 270         


Glu Ser Glu Cys Arg Val Val Lys Ser Thr Ser Tyr Thr Lys Ile Ala 
        275                 280                 285             


Ser Ser Ser Arg Arg Ser Thr Thr Lys Ser Pro Gly Pro Ser Arg Arg 
    290                 295                 300                 


Ser Lys Ser Pro Ala Ser Thr Ser Ser Val Asn Gly Thr Pro Gly Ser 
305                 310                 315                 320 


Gln Leu Ser Thr Pro Arg Ser Gly Lys Ser Pro Ser Pro Ser Pro Thr 
                325                 330                 335     


Ser Pro Gly Ser Leu Arg Lys Gln Arg Asp Leu Tyr Arg Pro Leu Ser 
            340                 345                 350         


Ser Asp Asp Leu Asp Ser Val Gly Asp Ser Val 
        355                 360             


<210>  5<211>  21<212>  DNA<213>  unknown<220><223>  siDCL-2 sense strand

<400>  5
caagaagacg gcucacucct t                                               21


<210>  6<211>  21<212>  DNA<213>  unknown<220><223>  siDCL-2 antisense strand

<400>  6
ggagugagcc gucuucuugt t                                               21


<210>  7<211>  21<212>  DNA<213>  unknown<220><223>  hu-siDCL-2 sense strand

<400>  7
caagaaaacg gcucauucct t                                               21


<210>  8<211>  21<212>  DNA<213>  unknown<220><223>  hu-siDCL-2 antisense strand

<400>  8
ggaaugagcc guuuucuugt t                                               21


<210>  9<211>  21<212>  DNA<213>  unknown<220><223>  siDCL-3 sense strand

<400>  9
gaaagccaag aagguucgat t                                               21


<210>  10<211>  21<212>  DNA<213>  unknown<220><223>  siDCL-3 antisense strand

<400>  10
tcgaaccuuc uuggcuuuct t                                               21


<210>  11<211>  21<212>  DNA<213>  unknown<220><223>  hu-siDCL-3 sense strand

<400>  11
gaaggccaag aaaguucgut t                                               21


<210>  12<211>  21<212>  DNA<213>  unknown<220><223>  hu-siDCL-3 antisense strand

<400>  12
acgaacuuuc uuggccuuct t                                               21


<210>  13<211>  20<212>  RNA<213>  unknown<220><223>  DCLex2C antisense RNA oligonucleotide

<400>  13
gcugggcagg ccauucacac                                                 20


<210>  14<211>  20<212>  RNA<213>  unknown<220><223>  hu-DCLex2C antisense RNA oligonucleotide

<400>  14
gcucggcagg ccguucaccc                                                 20


<210>  15<211>  20<212>  RNA<213>  unknown<220><223>  DCLex2D antisense RNA oligonucleotide

<400>  15
cuucucggag cugagugucu                                                 20


<210>  16<211>  20<212>  RNA<213>  unknown<220><223>  hu-  DCLex2D antisense RNA oligonucleotide

<400>  16
cuucucggag cugagcgucu                                                 20


<210>  17<211>  20<212>  DNA<213>  unknown<220><223>  DCLex2A antisense DNA oligonucleotide

<400>  17
gctgggcagg ccattcacac                                                 20


<210>  18<211>  20<212>  DNA<213>  unknown<220><223>  hu-DCLex2A antisense DNA oligonucleotide

<400>  18
gctcggcagg ccgttcaccc                                                 20


<210>  19<211>  20<212>  DNA<213>  unknown<220><223>  DCLex2B antisense DNA oligonucleotide

<400>  19
cttctcggag ctgagtgtct                                                 20


<210>  20<211>  20<212>  DNA<213>  unknown<220><223>  hu-DCLex2B antisense DNA oligonulceotide

<400>  20
cttctcggag ctgagcgtct                                                 20


