                         SEQUENCE LISTING

<110>  Teagasc Diary Products Research
 
<120>  Process for the production of  trans-10, cis 12 octadecadienoic 
       acid

<130>  AE 20051193, PF:57423

<140>  05113089.6
<141>  2005-12-24

<160>  5     

<170>  PatentIn version 3.3

<210>  1
<211>  1278
<212>  DNA
<213>  Propionibacterium acnes


<220>
<221>  CDS
<222>  (1)..(1275)

<400>  1
atg ggt tcc att tcc aag gac tcc aga att gct att att ggt gct ggc       48
Met Gly Ser Ile Ser Lys Asp Ser Arg Ile Ala Ile Ile Gly Ala Gly         
1               5                   10                  15              

ccg gcc ggg ctg gct gcc gga atg tac ctc gaa cag gcc gga ttt cac       96
Pro Ala Gly Leu Ala Ala Gly Met Tyr Leu Glu Gln Ala Gly Phe His         
            20                  25                  30                  

gac tac acg atc ctg gaa cgc acc gac cac gtc gga ggc aag tgc cac      144
Asp Tyr Thr Ile Leu Glu Arg Thr Asp His Val Gly Gly Lys Cys His         
        35                  40                  45                      

tca ccg aac tac cac ggc cgt cgt tat gag atg ggg gcc atc atg ggc      192
Ser Pro Asn Tyr His Gly Arg Arg Tyr Glu Met Gly Ala Ile Met Gly         
    50                  55                  60                          

gtc ccc agt tac gac acc atc cag gag atc atg gat cgc act ggc gac      240
Val Pro Ser Tyr Asp Thr Ile Gln Glu Ile Met Asp Arg Thr Gly Asp         
65                  70                  75                  80          

aag gtc gac ggg ccg aaa ctg cgt cgc gag ttc ctg cac gag gac ggc      288
Lys Val Asp Gly Pro Lys Leu Arg Arg Glu Phe Leu His Glu Asp Gly         
                85                  90                  95              

gag atc tac gtc ccg gaa aag gat cca gtg cgt ggt ccg cag gtc atg      336
Glu Ile Tyr Val Pro Glu Lys Asp Pro Val Arg Gly Pro Gln Val Met         
            100                 105                 110                 

gca gca gtg cag aag ctg ggc cag ttg ctc gcg acg aag tac cag gga      384
Ala Ala Val Gln Lys Leu Gly Gln Leu Leu Ala Thr Lys Tyr Gln Gly         
        115                 120                 125                     

tat gac gcc aac ggc cac tac aac aag gtt cac gag gac ctc atg ctg      432
Tyr Asp Ala Asn Gly His Tyr Asn Lys Val His Glu Asp Leu Met Leu         
    130                 135                 140                         

ccc ttc gac gag ttc ctc gcc ctc aac ggg tgc gag gcc gcc cga gac      480
Pro Phe Asp Glu Phe Leu Ala Leu Asn Gly Cys Glu Ala Ala Arg Asp         
145                 150                 155                 160         

ctg tgg atc aac ccc ttc acg gcc ttc ggc tac ggg cac ttc gac aac      528
Leu Trp Ile Asn Pro Phe Thr Ala Phe Gly Tyr Gly His Phe Asp Asn         
                165                 170                 175             

gtc ccg gcc gcc tac gtg ctg aag tac ctc gac ttc gtc acc atg atg      576
Val Pro Ala Ala Tyr Val Leu Lys Tyr Leu Asp Phe Val Thr Met Met         
            180                 185                 190                 

tcc ttt gcc aag gga gat ctg tgg acg tgg gcc gac ggc acc cag gcg      624
Ser Phe Ala Lys Gly Asp Leu Trp Thr Trp Ala Asp Gly Thr Gln Ala         
        195                 200                 205                     

atg ttc gag cac ctc aac gcc acc ctg gag cac ccg gcc gaa cgc aac      672
Met Phe Glu His Leu Asn Ala Thr Leu Glu His Pro Ala Glu Arg Asn         
    210                 215                 220                         

gtt gac atc act cgc atc acc cgc gag gac ggc aag gtc cac att cac      720
Val Asp Ile Thr Arg Ile Thr Arg Glu Asp Gly Lys Val His Ile His         
225                 230                 235                 240         

acc acg gac tgg gat cgc gag tcc gac gtc ctc gtc ctc acc gtc ccg      768
Thr Thr Asp Trp Asp Arg Glu Ser Asp Val Leu Val Leu Thr Val Pro         
                245                 250                 255             

ctg gaa aag ttc ctc gac tac tcc gac gcg gac gat gac gag cgg gag      816
Leu Glu Lys Phe Leu Asp Tyr Ser Asp Ala Asp Asp Asp Glu Arg Glu         
            260                 265                 270                 

tac ttc tcg aag atc atc cac cag cag tac atg gtg gat gcc tgc ctg      864
Tyr Phe Ser Lys Ile Ile His Gln Gln Tyr Met Val Asp Ala Cys Leu         
        275                 280                 285                     

gtg aag gag tac ccg acc atc tcc ggg tac gtc ccc gac aac atg agg      912
Val Lys Glu Tyr Pro Thr Ile Ser Gly Tyr Val Pro Asp Asn Met Arg         
    290                 295                 300                         

ccc gaa cgt ctc ggg cac gtc atg gtt tac tac cac cgc tgg gct gat      960
Pro Glu Arg Leu Gly His Val Met Val Tyr Tyr His Arg Trp Ala Asp         
305                 310                 315                 320         

gat ccg cac cag atc atc acg acc tac ctg cta cgt aac cat ccg gac     1008
Asp Pro His Gln Ile Ile Thr Thr Tyr Leu Leu Arg Asn His Pro Asp         
                325                 330                 335             

tac gcg gac aag act cag gag gag tgc cgc cag atg gtc ctc gac gac     1056
Tyr Ala Asp Lys Thr Gln Glu Glu Cys Arg Gln Met Val Leu Asp Asp         
            340                 345                 350                 

atg gag acc ttc ggt cat ccg gtc gag aag atc atc gag gag cag acc     1104
Met Glu Thr Phe Gly His Pro Val Glu Lys Ile Ile Glu Glu Gln Thr         
        355                 360                 365                     

tgg tac tac ttc ccg cac gtt agc tcg gag gac tac aag gcc ggg tgg     1152
Trp Tyr Tyr Phe Pro His Val Ser Ser Glu Asp Tyr Lys Ala Gly Trp         
    370                 375                 380                         

tac gag aag gtc gag gga atg cag ggt cgt cgc aac acc ttc tac gcc     1200
Tyr Glu Lys Val Glu Gly Met Gln Gly Arg Arg Asn Thr Phe Tyr Ala         
385                 390                 395                 400         

gga gaa att atg agt ttc ggt aat ttc gac gag gtg tgc cac tac tcg     1248
Gly Glu Ile Met Ser Phe Gly Asn Phe Asp Glu Val Cys His Tyr Ser         
                405                 410                 415             

aag gac ctg gtg acg cgg ttc ttc gtg tga                             1278
Lys Asp Leu Val Thr Arg Phe Phe Val                                     
            420                 425                                     


<210>  2
<211>  425
<212>  PRT
<213>  Propionibacterium acnes

<400>  2

Met Gly Ser Ile Ser Lys Asp Ser Arg Ile Ala Ile Ile Gly Ala Gly 
1               5                   10                  15      


Pro Ala Gly Leu Ala Ala Gly Met Tyr Leu Glu Gln Ala Gly Phe His 
            20                  25                  30          


Asp Tyr Thr Ile Leu Glu Arg Thr Asp His Val Gly Gly Lys Cys His 
        35                  40                  45              


Ser Pro Asn Tyr His Gly Arg Arg Tyr Glu Met Gly Ala Ile Met Gly 
    50                  55                  60                  


Val Pro Ser Tyr Asp Thr Ile Gln Glu Ile Met Asp Arg Thr Gly Asp 
65                  70                  75                  80  


Lys Val Asp Gly Pro Lys Leu Arg Arg Glu Phe Leu His Glu Asp Gly 
                85                  90                  95      


Glu Ile Tyr Val Pro Glu Lys Asp Pro Val Arg Gly Pro Gln Val Met 
            100                 105                 110         


Ala Ala Val Gln Lys Leu Gly Gln Leu Leu Ala Thr Lys Tyr Gln Gly 
        115                 120                 125             


Tyr Asp Ala Asn Gly His Tyr Asn Lys Val His Glu Asp Leu Met Leu 
    130                 135                 140                 


Pro Phe Asp Glu Phe Leu Ala Leu Asn Gly Cys Glu Ala Ala Arg Asp 
145                 150                 155                 160 


Leu Trp Ile Asn Pro Phe Thr Ala Phe Gly Tyr Gly His Phe Asp Asn 
                165                 170                 175     


Val Pro Ala Ala Tyr Val Leu Lys Tyr Leu Asp Phe Val Thr Met Met 
            180                 185                 190         


Ser Phe Ala Lys Gly Asp Leu Trp Thr Trp Ala Asp Gly Thr Gln Ala 
        195                 200                 205             


Met Phe Glu His Leu Asn Ala Thr Leu Glu His Pro Ala Glu Arg Asn 
    210                 215                 220                 


Val Asp Ile Thr Arg Ile Thr Arg Glu Asp Gly Lys Val His Ile His 
225                 230                 235                 240 


Thr Thr Asp Trp Asp Arg Glu Ser Asp Val Leu Val Leu Thr Val Pro 
                245                 250                 255     


Leu Glu Lys Phe Leu Asp Tyr Ser Asp Ala Asp Asp Asp Glu Arg Glu 
            260                 265                 270         


Tyr Phe Ser Lys Ile Ile His Gln Gln Tyr Met Val Asp Ala Cys Leu 
        275                 280                 285             


Val Lys Glu Tyr Pro Thr Ile Ser Gly Tyr Val Pro Asp Asn Met Arg 
    290                 295                 300                 


Pro Glu Arg Leu Gly His Val Met Val Tyr Tyr His Arg Trp Ala Asp 
305                 310                 315                 320 


Asp Pro His Gln Ile Ile Thr Thr Tyr Leu Leu Arg Asn His Pro Asp 
                325                 330                 335     


Tyr Ala Asp Lys Thr Gln Glu Glu Cys Arg Gln Met Val Leu Asp Asp 
            340                 345                 350         


Met Glu Thr Phe Gly His Pro Val Glu Lys Ile Ile Glu Glu Gln Thr 
        355                 360                 365             


Trp Tyr Tyr Phe Pro His Val Ser Ser Glu Asp Tyr Lys Ala Gly Trp 
    370                 375                 380                 


Tyr Glu Lys Val Glu Gly Met Gln Gly Arg Arg Asn Thr Phe Tyr Ala 
385                 390                 395                 400 


Gly Glu Ile Met Ser Phe Gly Asn Phe Asp Glu Val Cys His Tyr Ser 
                405                 410                 415     


Lys Asp Leu Val Thr Arg Phe Phe Val 
            420                 425 


<210>  3
<211>  43
<212>  DNA
<213>  artificial sequence

<220>  
<223>  primer sequence

<400>  3
aaaactgcag aggaggaaaa aaaatgggtt ccatttccaa gga                       43


<210>  4
<211>  29
<212>  DNA
<213>  artificial sequence

<220>  
<223>  primer sequence

<400>  4
cggggtacct cacacgaaga accgcgtca                                       29


<210>  5
<211>  3394
<212>  DNA
<213>  artificial sequence

<220>  
<223>  vector

<400>  5
taacaattgt aacccataca ggagaaggga cgatagcaat tttttcaata agtagacaaa     60

gtagagaata atttaataaa aaactgaaaa aatcacagct aaactcttgt tttacttgat    120

tttatgttaa aataattaat gagtgtaatt gtatataaaa ttatctgtac acttacctaa    180

tttattaaaa aaaaatatga atcgtgatgt gtgagggaaa ggagtcgctt ttatggccaa    240

accatgggta ctgcaggcat gcggtaccac tagttctaga gagctcaagc tttctttgaa    300

ccaaaattag aaaaccaagg cttgaaacgt tcaattgaaa tggcaattaa acaaattaca    360

gcacgtgttg ctttgattga tagccaaaaa gcagcagttg ataaagcaat tactgatatt    420

gctgaaaaat tgtaatttat aaataaaaat caccttttag aggtggtttt tttatttata    480

aattattcgt ttgatttcgc tttcgataga acaatcaaag cgagaataag gaagataaat    540

cccataaggg cgggagcaga atgtccgaga ctaatgtaaa tttgtccacc aattaaagga    600

ccgataacgc gagcttctcg agtgcatatt ttcggcaatc ttctcaatga gatgctcttc    660

agcatgttca atgatgtcga ttttttatta aaacgtctca aaatcgtttc tgagacgttt    720

tagcgtttat ttcgtttagt tatcggcata atcgttaaaa caggcgttat cgtagcgtaa    780

aagcccttga gcgtagcgtg ctttgcagcg aagatgttgt ctgttagatt atgaaagccg    840

atgactgaat gaaataataa gcgcagcgtc cttctatttc ggttggagga ggctcaaggg    900

agtttgaggg aatgaaattc cctcatgggt ttgattttaa aaattgcttg caattttgcc    960

gagcggtagc gctggaaaat ttttgaaaaa aatttggaat ttggaaaaaa atggggggaa   1020

aggaagcgaa ttttgcttcc gtactacgac cccccattaa gtgccgagtg ccaatttttg   1080

tgccaaaaac gctctatccc aactggctca agggtttgag gggtttttca atcgccaacg   1140

aatcgccaac gttttcgcca acgtttttta taaatctata tttaagtagc tttattgttg   1200

tttttatgat tacaaagtga tacactaatt ttataaaatt atttgattgg agttttttaa   1260

atggtgattt cagaatcgaa aaaaagagtt atgatttctc tgacaaaaga gcaagataaa   1320

aaattaacag atatggcgaa acaaaaaggt ttttcaaaat ctgcggttgc ggcgttagct   1380

atagaagaat atgcaagaaa ggaatcagaa caaaaaaaat aagcgaaagc tcgcgttttt   1440

agaaggatac gagttttcgc tacttgtttt tgataaggta atatatcatg gctattaaaa   1500

atactaaagc tagaaatttt ggatttttat tatatcctga ctcaattcct aatgattgga   1560

aagaaaaatt agagagtttg ggcgtatcta tggctgtcag tcctttacac gatatggacg   1620

aaaaaaaaga taaagataca tggaatagta gtgatgttat acgaaatgga aagcactata   1680

aaaaaccaca ctatcacgtt atatatattg cacgaaatcc tgtaacaata gaaagcgtta   1740

ggaacaagat taagcgaaaa ttggggaata gttcagttgc tcatgttgag atacttgatt   1800

atatcaaagg ttcatatgaa tatttgactc atgaatcaaa ggacgctatt gctaagaata   1860

aacatatata cgacaaaaaa gatattttga acattaatga ttttgatatt gaccgctata   1920

taacacttga tgaaagccaa aaaagagaat tgaagaattt acttttagat atagtggatg   1980

actataattt ggtaaataca aaagatttaa tggcttttat tcgccttagg ggagcggagt   2040

ttggaatttt aaatacgaat gatgtaaaag atattgtttc aacaaactct agcgccttta   2100

gattatggtt tgagggcaat tatcagtgtg gatatagagc aagttatgca aaggttcttg   2160

atgctgaaac gggggaaata aaatgacaaa caaagaaaaa gagttatttg ctgaaaatga   2220

ggaattaaaa aaagaaatta aggacttaaa agagcgtatt gaaagataca gagaaatgga   2280

agttgaatta agtacaacaa tagatttatt gagaggaggg attattgaat aaataaaagc   2340

ccccctgacg aaagtcgacg gcaatagtta cccttattat caagataaga aagaaaagga   2400

tttttcgcta cgctcaaatc ctttaaaaaa acacaaaaga ccacattttt taatgtggtc   2460

tttattcttc aactaaagca cccattagtt caacaaacga aaattggata aagtgggata   2520

tttttaaaat atatatttat gttacagtaa tattgacttt taaaaaagga ttgattctaa   2580

tgaagaaagc agacaagtaa gcctcctaaa ttcactttag ataaaaattt aggaggcata   2640

tcaaatgaac tttaataaaa ttgatttaga caattggaag agaaaagaga tatttaatca   2700

ttatttgaac caacaaacga cttttagtat aaccacagaa attgatatta gtgttttata   2760

ccgaaacata aaacaagaag gatataaatt ttaccctgca tttattttct tagtgacaag   2820

ggtgataaac tcaaatacag cttttagaac tggttacaat agcgacggag agttaggtta   2880

ttgggataag ttagagccac tttatacaat ttttgatggt gtatctaaaa cattctctgg   2940

tatttggact cctgtaaaga atgacttcaa agagttttat gatttatacc tttctgatgt   3000

agagaaatat aatggttcgg ggaaattgtt tcccaaaaca cctatacctg aaaatgcttt   3060

ttctctttct attattcctt ggacttcatt tactgggttt aacttaaata tcaataataa   3120

tagtaattac cttctaccca ttattacagc aggaaaattc attaataaag gtaattcaat   3180

atatttaccg ctatctttac aggtacatca ttctgtttgt gatggttatc atgcaggatt   3240

gtttatgaac tctattcagg aattgtcaga taggcctaat gactggcttt tataatatga   3300

gataatgccg actgtacttt ttacagtcgg ttttctaatg tcactaacct gccccgttag   3360

ttgaagaagg tttttatatt acagctccaa gatc                               3394


