                         SEQUENCE LISTING

<110>  Universita' degli Studi di Torino
 
<120>  Regulation of expression of P130Cas/BCAR1 protein in tumors

<130>  BEP8032-CF

<160>  9     

<170>  PatentIn version 3.3

<210>  1
<211>  870
<212>  PRT
<213>  artificial

<220>
<223>  p130Cas protein

<400>  1

Met Asn His Leu Asn Val Leu Ala Lys Ala Leu Tyr Asp Asn Val Ala 
1               5                   10                  15      


Glu Ser Pro Asp Glu Leu Ser Phe Arg Lys Gly Asp Ile Met Thr Val 
            20                  25                  30          


Leu Glu Gln Asp Thr Gln Gly Leu Asp Gly Trp Trp Leu Cys Ser Leu 
        35                  40                  45              


His Gly Arg Gln Gly Ile Val Pro Gly Asn Arg Leu Lys Ile Leu Val 
    50                  55                  60                  


Gly Met Tyr Asp Lys Lys Pro Ala Gly Pro Gly Pro Gly Pro Pro Ala 
65                  70                  75                  80  


Thr Pro Ala Gln Pro Gln Pro Gly Leu His Ala Pro Ala Pro Pro Ala 
                85                  90                  95      


Ser Gln Tyr Thr Pro Met Leu Pro Asn Thr Tyr Gln Pro Gln Pro Asp 
            100                 105                 110         


Ser Val Tyr Leu Val Pro Thr Pro Ser Lys Ala Gln Gln Gly Leu Tyr 
        115                 120                 125             


Gln Val Pro Gly Pro Ser Pro Gln Phe Gln Ser Pro Pro Ala Lys Gln 
    130                 135                 140                 


Thr Ser Thr Phe Ser Lys Gln Thr Pro His His Pro Phe Pro Ser Pro 
145                 150                 155                 160 


Ala Thr Asp Leu Tyr Gln Val Pro Pro Gly Pro Gly Gly Pro Ala Gln 
                165                 170                 175     


Asp Ile Tyr Gln Val Pro Pro Ser Ala Gly Met Gly His Asp Ile Tyr 
            180                 185                 190         


Gln Val Pro Pro Ser Met Asp Thr Arg Ser Trp Glu Gly Thr Lys Pro 
        195                 200                 205             


Pro Ala Lys Val Val Val Pro Thr Arg Val Gly Gln Gly Tyr Val Tyr 
    210                 215                 220                 


Glu Ala Ala Gln Pro Glu Gln Asp Glu Tyr Asp Ile Pro Arg His Leu 
225                 230                 235                 240 


Leu Ala Pro Gly Pro Gln Asp Ile Tyr Asp Val Pro Pro Val Arg Gly 
                245                 250                 255     


Leu Leu Pro Ser Gln Tyr Gly Gln Glu Val Tyr Asp Thr Pro Pro Met 
            260                 265                 270         


Ala Val Lys Gly Pro Asn Gly Arg Asp Pro Leu Leu Glu Val Tyr Asp 
        275                 280                 285             


Val Pro Pro Ser Val Glu Lys Gly Leu Pro Pro Ser Asn His His Ala 
    290                 295                 300                 


Val Tyr Asp Val Pro Pro Ser Val Ser Lys Asp Val Pro Asp Gly Pro 
305                 310                 315                 320 


Leu Leu Arg Glu Glu Thr Tyr Asp Val Pro Pro Ala Phe Ala Lys Ala 
                325                 330                 335     


Lys Pro Phe Asp Pro Ala Arg Thr Pro Leu Val Leu Ala Ala Pro Pro 
            340                 345                 350         


Pro Asp Ser Pro Pro Ala Glu Asp Val Tyr Asp Val Pro Pro Pro Ala 
        355                 360                 365             


Pro Asp Leu Tyr Asp Val Pro Pro Gly Leu Arg Arg Pro Gly Pro Gly 
    370                 375                 380                 


Thr Leu Tyr Asp Val Pro Arg Glu Arg Val Leu Pro Pro Glu Val Ala 
385                 390                 395                 400 


Asp Gly Gly Val Val Asp Ser Gly Val Tyr Ala Val Pro Pro Pro Ala 
                405                 410                 415     


Glu Arg Glu Ala Pro Ala Glu Gly Lys Arg Leu Ser Ala Ser Ser Thr 
            420                 425                 430         


Gly Ser Thr Arg Ser Ser Gln Ser Ala Ser Ser Leu Glu Val Ala Gly 
        435                 440                 445             


Pro Gly Arg Glu Pro Leu Glu Leu Glu Val Ala Val Glu Ala Leu Ala 
    450                 455                 460                 


Arg Leu Gln Gln Gly Val Ser Ala Thr Val Ala His Leu Leu Asp Leu 
465                 470                 475                 480 


Ala Gly Ser Ala Gly Ala Thr Gly Ser Trp Arg Ser Pro Ser Glu Pro 
                485                 490                 495     


Gln Glu Pro Leu Val Gln Asp Leu Gln Ala Ala Val Ala Ala Val Gln 
            500                 505                 510         


Ser Ala Val His Glu Leu Leu Glu Phe Ala Arg Ser Ala Val Gly Asn 
        515                 520                 525             


Ala Ala His Thr Ser Asp Arg Ala Leu His Ala Lys Leu Ser Arg Gln 
    530                 535                 540                 


Leu Gln Lys Met Glu Asp Val His Gln Thr Leu Val Ala His Gly Gln 
545                 550                 555                 560 


Ala Leu Asp Ala Gly Arg Gly Gly Ser Gly Ala Thr Leu Glu Asp Leu 
                565                 570                 575     


Asp Arg Leu Val Ala Cys Ser Arg Ala Val Pro Glu Asp Ala Lys Gln 
            580                 585                 590         


Leu Ala Ser Phe Leu His Gly Asn Ala Ser Leu Leu Phe Arg Arg Thr 
        595                 600                 605             


Lys Ala Thr Ala Pro Gly Pro Glu Gly Gly Gly Thr Leu His Pro Asn 
    610                 615                 620                 


Pro Thr Asp Lys Thr Ser Ser Ile Gln Ser Arg Pro Leu Pro Ser Pro 
625                 630                 635                 640 


Pro Lys Phe Thr Ser Gln Asp Ser Pro Asp Gly Gln Tyr Glu Asn Ser 
                645                 650                 655     


Glu Gly Gly Trp Met Glu Asp Tyr Asp Tyr Val His Leu Gln Gly Lys 
            660                 665                 670         


Glu Glu Phe Glu Lys Thr Gln Lys Glu Leu Leu Glu Lys Gly Ser Ile 
        675                 680                 685             


Thr Arg Gln Gly Lys Ser Gln Leu Glu Leu Gln Gln Leu Lys Gln Phe 
    690                 695                 700                 


Glu Arg Leu Glu Gln Glu Val Ser Arg Pro Ile Asp His Asp Leu Ala 
705                 710                 715                 720 


Asn Trp Thr Pro Ala Gln Pro Leu Ala Pro Gly Arg Thr Gly Gly Leu 
                725                 730                 735     


Gly Pro Ser Asp Arg Gln Leu Leu Leu Phe Tyr Leu Glu Gln Cys Glu 
            740                 745                 750         


Ala Asn Leu Thr Thr Leu Thr Asn Ala Val Asp Ala Phe Phe Thr Ala 
        755                 760                 765             


Val Ala Thr Asn Gln Pro Pro Lys Ile Phe Val Ala His Ser Lys Phe 
    770                 775                 780                 


Val Ile Leu Ser Ala His Lys Leu Val Phe Ile Gly Asp Thr Leu Ser 
785                 790                 795                 800 


Arg Gln Ala Lys Ala Ala Asp Val Arg Ser Gln Val Thr His Tyr Ser 
                805                 810                 815     


Asn Leu Leu Cys Asp Leu Leu Arg Gly Ile Val Ala Thr Thr Lys Ala 
            820                 825                 830         


Ala Ala Leu Gln Tyr Pro Ser Pro Ser Ala Ala Gln Asp Met Val Glu 
        835                 840                 845             


Arg Val Lys Glu Leu Gly His Ser Thr Gln Gln Phe Arg Arg Val Leu 
    850                 855                 860                 


Gly Gln Leu Ala Ala Ala 
865                 870 


<210>  2
<211>  456
<212>  PRT
<213>  artificial

<220>
<223>  p130Cas C-term

<400>  2

Pro Ala Glu Arg Glu Ala Pro Ala Glu Gly Lys Arg Leu Ser Ala Ser 
1               5                   10                  15      


Ser Thr Gly Ser Thr Arg Ser Ser Gln Ser Ala Ser Ser Leu Glu Val 
            20                  25                  30          


Ala Gly Pro Gly Arg Glu Pro Leu Glu Leu Glu Val Ala Val Glu Ala 
        35                  40                  45              


Leu Ala Arg Leu Gln Gln Gly Val Ser Ala Thr Val Ala His Leu Leu 
    50                  55                  60                  


Asp Leu Ala Gly Ser Ala Gly Ala Thr Gly Ser Trp Arg Ser Pro Ser 
65                  70                  75                  80  


Glu Pro Gln Glu Pro Leu Val Gln Asp Leu Gln Ala Ala Val Ala Ala 
                85                  90                  95      


Val Gln Ser Ala Val His Glu Leu Leu Glu Phe Ala Arg Ser Ala Val 
            100                 105                 110         


Gly Asn Ala Ala His Thr Ser Asp Arg Ala Leu His Ala Lys Leu Ser 
        115                 120                 125             


Arg Gln Leu Gln Lys Met Glu Asp Val His Gln Thr Leu Val Ala His 
    130                 135                 140                 


Gly Gln Ala Leu Asp Ala Gly Arg Gly Gly Ser Gly Ala Thr Leu Glu 
145                 150                 155                 160 


Asp Leu Asp Arg Leu Val Ala Cys Ser Arg Ala Val Pro Glu Asp Ala 
                165                 170                 175     


Lys Gln Leu Ala Ser Phe Leu His Gly Asn Ala Ser Leu Leu Phe Arg 
            180                 185                 190         


Arg Thr Lys Ala Thr Ala Pro Gly Pro Glu Gly Gly Gly Thr Leu His 
        195                 200                 205             


Pro Asn Pro Thr Asp Lys Thr Ser Ser Ile Gln Ser Arg Pro Leu Pro 
    210                 215                 220                 


Ser Pro Pro Lys Phe Thr Ser Gln Asp Ser Pro Asp Gly Gln Tyr Glu 
225                 230                 235                 240 


Asn Ser Glu Gly Gly Trp Met Glu Asp Tyr Asp Tyr Val His Leu Gln 
                245                 250                 255     


Gly Lys Glu Glu Phe Glu Lys Thr Gln Lys Glu Leu Leu Glu Lys Gly 
            260                 265                 270         


Ser Ile Thr Arg Gln Gly Lys Ser Gln Leu Glu Leu Gln Gln Leu Lys 
        275                 280                 285             


Gln Phe Glu Arg Leu Glu Gln Glu Val Ser Arg Pro Ile Asp His Asp 
    290                 295                 300                 


Leu Ala Asn Trp Thr Pro Ala Gln Pro Leu Ala Pro Gly Arg Thr Gly 
305                 310                 315                 320 


Gly Leu Gly Pro Ser Asp Arg Gln Leu Leu Leu Phe Tyr Leu Glu Gln 
                325                 330                 335     


Cys Glu Ala Asn Leu Thr Thr Leu Thr Asn Ala Val Asp Ala Phe Phe 
            340                 345                 350         


Thr Ala Val Ala Thr Asn Gln Pro Pro Lys Ile Phe Val Ala His Ser 
        355                 360                 365             


Lys Phe Val Ile Leu Ser Ala His Lys Leu Val Phe Ile Gly Asp Thr 
    370                 375                 380                 


Leu Ser Arg Gln Ala Lys Ala Ala Asp Val Arg Ser Gln Val Thr His 
385                 390                 395                 400 


Tyr Ser Asn Leu Leu Cys Asp Leu Leu Arg Gly Ile Val Ala Thr Thr 
                405                 410                 415     


Lys Ala Ala Ala Leu Gln Tyr Pro Ser Pro Ser Ala Ala Gln Asp Met 
            420                 425                 430         


Val Glu Arg Val Lys Glu Leu Gly His Ser Thr Gln Gln Phe Arg Arg 
        435                 440                 445             


Val Leu Gly Gln Leu Ala Ala Ala 
    450                 455     


<210>  3
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  PCR primer

<400>  3
ggccaccaac caaccacc                                                   18


<210>  4
<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  PCR primer

<400>  4
cggcgctatt cagatcctct tct                                             23


<210>  5
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  p130Cas siRNA

<400>  5
ttgactaata gtctacattt a                                               21


<210>  6
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  control siRNA

<400>  6
aatcatttga ctaatagtct a                                               21


<210>  7
<211>  360
<212>  DNA
<213>  artificial

<220>
<223>  probe - C-term

<400>  7
agccagtatg gccaggaggt gtatgacaca ccccccatgg ctgtcaaggg tcccaatggc     60

cgagacccgt tgctggaggt gtatgacgtg ccccccagtg tggagaaggg cctgccaccg    120

tccaaccacc acgcagtcta cgacgttcct ccatcggtga gcaaggatgt gcccgatggc    180

ccactgctgc gtgaggagac ctacgatgtg ccccccgcct tcgccaaggc caagcccttt    240

gacccggccc gcaccccact ggtactggct gcgccccctc cagactcccc gccggccgag    300

gacgtgtatg acgtgccgcc cccggctcct gacctctacg acgtgccccc tggcttgcgg    360


<210>  8
<211>  38
<212>  DNA
<213>  artificial

<220>
<223>  p130Cas shRNA

<400>  8
gcccagccac ggacctttat aaaggtccgt ggctgggc                             38


<210>  9
<211>  9
<212>  DNA
<213>  artificial

<220>
<223>  linker

<400>  9
tctcttgaa                                                              9


