                         SEQUENCE LISTING

<110>  Novozymes A/S
 
<120>  Endoglucanase

<130>  10780.204-WO

<160>  4     

<170>  PatentIn version 3.3

<210>  1
<211>  2379
<212>  DNA
<213>  Bacillus sp. ACE160


<220>
<221>  CDS
<222>  (1)..(2376)

<220>
<221>  sig_peptide
<222>  (1)..(99)

<220>
<221>  mat_peptide
<222>  (100)..(2376)

<400>  1
gtg aga caa ccc ata ggt aaa aag ata att gct gca gga atg atc ttt       48
Val Arg Gln Pro Ile Gly Lys Lys Ile Ile Ala Ala Gly Met Ile Phe         
            -30                 -25                 -20                 

acc ctc tta ttt tcg tta atc gtc act gtg ttc cca act gct ggt caa       96
Thr Leu Leu Phe Ser Leu Ile Val Thr Val Phe Pro Thr Ala Gly Gln         
        -15                 -10                 -5                      

gca cta gaa tca gac tat agc cat tta tta gga aat gat gca gtg aag      144
Ala Leu Glu Ser Asp Tyr Ser His Leu Leu Gly Asn Asp Ala Val Lys         
-1  1               5                   10                  15          

cgc ccc tcg gaa ggc gga gct tta agt tta tgt aat gaa act act cca      192
Arg Pro Ser Glu Gly Gly Ala Leu Ser Leu Cys Asn Glu Thr Thr Pro         
                20                  25                  30              

gta aaa cca aac cat gcg ggg gac cgt ggg aaa cca agc cac gca ggt      240
Val Lys Pro Asn His Ala Gly Asp Arg Gly Lys Pro Ser His Ala Gly         
            35                  40                  45                  

aaa gga aag cct ccc cat gct ggt aag cct gaa cat gcc gga cca aag      288
Lys Gly Lys Pro Pro His Ala Gly Lys Pro Glu His Ala Gly Pro Lys         
        50                  55                  60                      

cgt aaa aca ctg tgt gat gca acc ggc agc caa att cag ctc cgg ggg      336
Arg Lys Thr Leu Cys Asp Ala Thr Gly Ser Gln Ile Gln Leu Arg Gly         
    65                  70                  75                          

atg agc act cac gga ttg caa tgg ttt ggc gag att ata aat gat aat      384
Met Ser Thr His Gly Leu Gln Trp Phe Gly Glu Ile Ile Asn Asp Asn         
80                  85                  90                  95          

gct ttt gct gct ctt tcc aac gac tgg gag gca aat atg atc cgc ctt      432
Ala Phe Ala Ala Leu Ser Asn Asp Trp Glu Ala Asn Met Ile Arg Leu         
                100                 105                 110             

gcc atg tat att ggc gaa aat gga tat gcg act aac cct gaa gta aaa      480
Ala Met Tyr Ile Gly Glu Asn Gly Tyr Ala Thr Asn Pro Glu Val Lys         
            115                 120                 125                 

gaa ctt gtt tac gaa gga att gag ctt gca ttc aaa cat gat atg tat      528
Glu Leu Val Tyr Glu Gly Ile Glu Leu Ala Phe Lys His Asp Met Tyr         
        130                 135                 140                     

gta att gtt gac tgg cac gta cac gcc ccg gga gat cca agg gct gac      576
Val Ile Val Asp Trp His Val His Ala Pro Gly Asp Pro Arg Ala Asp         
    145                 150                 155                         

att tat tcc ggt gct cta gac ttt ttt aaa gaa att gca gac cac tat      624
Ile Tyr Ser Gly Ala Leu Asp Phe Phe Lys Glu Ile Ala Asp His Tyr         
160                 165                 170                 175         

aag gac cat cct aag ttc cat tat att ata tgg gaa att gca aat gaa      672
Lys Asp His Pro Lys Phe His Tyr Ile Ile Trp Glu Ile Ala Asn Glu         
                180                 185                 190             

cca agc cca aat aac agc gga gga cct gga att cct aat gat gaa aca      720
Pro Ser Pro Asn Asn Ser Gly Gly Pro Gly Ile Pro Asn Asp Glu Thr         
            195                 200                 205                 

gga tgg aaa gca gta aag gaa tat gct gaa cct atc gtg gaa atg ctt      768
Gly Trp Lys Ala Val Lys Glu Tyr Ala Glu Pro Ile Val Glu Met Leu         
        210                 215                 220                     

cgt gaa agg ggg gac aat ata att ctt gta ggc agc ccg aac tgg agc      816
Arg Glu Arg Gly Asp Asn Ile Ile Leu Val Gly Ser Pro Asn Trp Ser         
    225                 230                 235                         

cag cgc ccg gat tta gct gca gat aac cct ata gat gca aaa aat atc      864
Gln Arg Pro Asp Leu Ala Ala Asp Asn Pro Ile Asp Ala Lys Asn Ile         
240                 245                 250                 255         

atg tac tct gtc cac ttc tat act gga tct cat gaa cct tca gat aca      912
Met Tyr Ser Val His Phe Tyr Thr Gly Ser His Glu Pro Ser Asp Thr         
                260                 265                 270             

agc tat cct gaa ggc act ccg tcc tcg gaa cgg aat aac gtt atg gca      960
Ser Tyr Pro Glu Gly Thr Pro Ser Ser Glu Arg Asn Asn Val Met Ala         
            275                 280                 285                 

aat gta cga tat gca ctc gag aat ggt gct gca gtt ttt gct aca gaa     1008
Asn Val Arg Tyr Ala Leu Glu Asn Gly Ala Ala Val Phe Ala Thr Glu         
        290                 295                 300                     

tgg ggt aca agc caa gcc aat ggt gat ggc ggc cca tac ctt gac gaa     1056
Trp Gly Thr Ser Gln Ala Asn Gly Asp Gly Gly Pro Tyr Leu Asp Glu         
    305                 310                 315                         

gct gat gta tgg ctt aac ttc ctt aac gag aac aat atc agc tgg gtc     1104
Ala Asp Val Trp Leu Asn Phe Leu Asn Glu Asn Asn Ile Ser Trp Val         
320                 325                 330                 335         

aac tgg tca ttg aca aat aaa aac gaa aca tca ggt tcc ttc act ccc     1152
Asn Trp Ser Leu Thr Asn Lys Asn Glu Thr Ser Gly Ser Phe Thr Pro         
                340                 345                 350             

ttt gag ctg ggg aaa tcc aat gcc aca agt ctt gat cct ggc cct gaa     1200
Phe Glu Leu Gly Lys Ser Asn Ala Thr Ser Leu Asp Pro Gly Pro Glu         
            355                 360                 365                 

caa gca tgg tcc ctg ccg gaa cta agc gta tca ggc gag tat gtt cgt     1248
Gln Ala Trp Ser Leu Pro Glu Leu Ser Val Ser Gly Glu Tyr Val Arg         
        370                 375                 380                     

tca cga att aaa ggt agt ccg tat gaa ccg ttt gac cgg acg aaa ttt     1296
Ser Arg Ile Lys Gly Ser Pro Tyr Glu Pro Phe Asp Arg Thr Lys Phe         
    385                 390                 395                         

aat aaa gta atc tgg gat ttt aac gac ggt aca gtt cag ggg ttt gaa     1344
Asn Lys Val Ile Trp Asp Phe Asn Asp Gly Thr Val Gln Gly Phe Glu         
400                 405                 410                 415         

gta aat gac gac agt cct gtt aaa gaa gaa ata gct gtc agc aat gca     1392
Val Asn Asp Asp Ser Pro Val Lys Glu Glu Ile Ala Val Ser Asn Ala         
                420                 425                 430             

ggt aat gcc ctt caa att acc ggt ctt aat gct agc aac gac atc tcc     1440
Gly Asn Ala Leu Gln Ile Thr Gly Leu Asn Ala Ser Asn Asp Ile Ser         
            435                 440                 445                 

aca gat aac ttc tgg agt aac ctc agg ctt tca gcc aat tcc tgg gga     1488
Thr Asp Asn Phe Trp Ser Asn Leu Arg Leu Ser Ala Asn Ser Trp Gly         
        450                 455                 460                     

gaa tcg gtt aat atc ctg ggg gca gaa gaa ctg aca tta gat gtg atc     1536
Glu Ser Val Asn Ile Leu Gly Ala Glu Glu Leu Thr Leu Asp Val Ile         
    465                 470                 475                         

gtc gat gag cca act tcc gtc tca atc gca gca att ccg caa agt gca     1584
Val Asp Glu Pro Thr Ser Val Ser Ile Ala Ala Ile Pro Gln Ser Ala         
480                 485                 490                 495         

gca gtt ggc tgg gca aat cct aac aat gcg gtc gtt gtt tct aaa gaa     1632
Ala Val Gly Trp Ala Asn Pro Asn Asn Ala Val Val Val Ser Lys Glu         
                500                 505                 510             

gat ttc gca cct tat ggt ggc cag tat aag gct gtc ctg acg ata aca     1680
Asp Phe Ala Pro Tyr Gly Gly Gln Tyr Lys Ala Val Leu Thr Ile Thr         
            515                 520                 525                 

ccg gaa gat tct cca gct ctg ggt gcc ata gca aca cat agt gat gat     1728
Pro Glu Asp Ser Pro Ala Leu Gly Ala Ile Ala Thr His Ser Asp Asp         
        530                 535                 540                     

aac atg atg aat aac att atc ttg ttt ata ggt aca gaa aat gct gat     1776
Asn Met Met Asn Asn Ile Ile Leu Phe Ile Gly Thr Glu Asn Ala Asp         
    545                 550                 555                         

gta ctc tca ctt gat aac att aca gtt aaa ggt tcc atc gtt gaa att     1824
Val Leu Ser Leu Asp Asn Ile Thr Val Lys Gly Ser Ile Val Glu Ile         
560                 565                 570                 575         

cca gta atc cat gat cca aag ggc atc gcg gtt ctt cct tca aac ttt     1872
Pro Val Ile His Asp Pro Lys Gly Ile Ala Val Leu Pro Ser Asn Phe         
                580                 585                 590             

gag gac gga acc cgc caa ggc tgg gac tgg aac cct gaa tca ggg gta     1920
Glu Asp Gly Thr Arg Gln Gly Trp Asp Trp Asn Pro Glu Ser Gly Val         
            595                 600                 605                 

aaa act gct tta aca att aaa gag gcc gat ggt tca cat gcc cta tcc     1968
Lys Thr Ala Leu Thr Ile Lys Glu Ala Asp Gly Ser His Ala Leu Ser         
        610                 615                 620                     

tgg gag ttt gct tac ccg gaa gtt aaa cct ggt gat ggg tgg gca aca     2016
Trp Glu Phe Ala Tyr Pro Glu Val Lys Pro Gly Asp Gly Trp Ala Thr         
    625                 630                 635                         

gct cca cga ttg gag ttt tgg aaa gat gga ctg gta aga gga gca aac     2064
Ala Pro Arg Leu Glu Phe Trp Lys Asp Gly Leu Val Arg Gly Ala Asn         
640                 645                 650                 655         

gat tac ctc tca ttt gat tta tac ctt gac cct gtc cgt gcc aca gag     2112
Asp Tyr Leu Ser Phe Asp Leu Tyr Leu Asp Pro Val Arg Ala Thr Glu         
                660                 665                 670             

ggt gct atc aca aca cat ctc gta ttc cag ccg cca agt gct gga tac     2160
Gly Ala Ile Thr Thr His Leu Val Phe Gln Pro Pro Ser Ala Gly Tyr         
            675                 680                 685                 

tgg gta caa gct cca gct tct cac agc ata gat ttg tta aac tta gat     2208
Trp Val Gln Ala Pro Ala Ser His Ser Ile Asp Leu Leu Asn Leu Asp         
        690                 695                 700                     

tca gct gat atc aca gca gat gga ctg tac cat tat gaa gtg aaa ttc     2256
Ser Ala Asp Ile Thr Ala Asp Gly Leu Tyr His Tyr Glu Val Lys Phe         
    705                 710                 715                         

aat att aga gac att aca gca att caa gat gac aca gct ctg cgc aat     2304
Asn Ile Arg Asp Ile Thr Ala Ile Gln Asp Asp Thr Ala Leu Arg Asn         
720                 725                 730                 735         

atg atc ctc ata ttg gag gat agg aac agc gac ttc gcg ggc cgg gcg     2352
Met Ile Leu Ile Leu Glu Asp Arg Asn Ser Asp Phe Ala Gly Arg Ala         
                740                 745                 750             

ttc atc gac aat gta aga ttc gaa taa                                 2379
Phe Ile Asp Asn Val Arg Phe Glu                                         
            755                                                         


<210>  2
<211>  792
<212>  PRT
<213>  Bacillus sp. ACE160

<400>  2

Val Arg Gln Pro Ile Gly Lys Lys Ile Ile Ala Ala Gly Met Ile Phe 
            -30                 -25                 -20         


Thr Leu Leu Phe Ser Leu Ile Val Thr Val Phe Pro Thr Ala Gly Gln 
        -15                 -10                 -5              


Ala Leu Glu Ser Asp Tyr Ser His Leu Leu Gly Asn Asp Ala Val Lys 
-1  1               5                   10                  15  


Arg Pro Ser Glu Gly Gly Ala Leu Ser Leu Cys Asn Glu Thr Thr Pro 
                20                  25                  30      


Val Lys Pro Asn His Ala Gly Asp Arg Gly Lys Pro Ser His Ala Gly 
            35                  40                  45          


Lys Gly Lys Pro Pro His Ala Gly Lys Pro Glu His Ala Gly Pro Lys 
        50                  55                  60              


Arg Lys Thr Leu Cys Asp Ala Thr Gly Ser Gln Ile Gln Leu Arg Gly 
    65                  70                  75                  


Met Ser Thr His Gly Leu Gln Trp Phe Gly Glu Ile Ile Asn Asp Asn 
80                  85                  90                  95  


Ala Phe Ala Ala Leu Ser Asn Asp Trp Glu Ala Asn Met Ile Arg Leu 
                100                 105                 110     


Ala Met Tyr Ile Gly Glu Asn Gly Tyr Ala Thr Asn Pro Glu Val Lys 
            115                 120                 125         


Glu Leu Val Tyr Glu Gly Ile Glu Leu Ala Phe Lys His Asp Met Tyr 
        130                 135                 140             


Val Ile Val Asp Trp His Val His Ala Pro Gly Asp Pro Arg Ala Asp 
    145                 150                 155                 


Ile Tyr Ser Gly Ala Leu Asp Phe Phe Lys Glu Ile Ala Asp His Tyr 
160                 165                 170                 175 


Lys Asp His Pro Lys Phe His Tyr Ile Ile Trp Glu Ile Ala Asn Glu 
                180                 185                 190     


Pro Ser Pro Asn Asn Ser Gly Gly Pro Gly Ile Pro Asn Asp Glu Thr 
            195                 200                 205         


Gly Trp Lys Ala Val Lys Glu Tyr Ala Glu Pro Ile Val Glu Met Leu 
        210                 215                 220             


Arg Glu Arg Gly Asp Asn Ile Ile Leu Val Gly Ser Pro Asn Trp Ser 
    225                 230                 235                 


Gln Arg Pro Asp Leu Ala Ala Asp Asn Pro Ile Asp Ala Lys Asn Ile 
240                 245                 250                 255 


Met Tyr Ser Val His Phe Tyr Thr Gly Ser His Glu Pro Ser Asp Thr 
                260                 265                 270     


Ser Tyr Pro Glu Gly Thr Pro Ser Ser Glu Arg Asn Asn Val Met Ala 
            275                 280                 285         


Asn Val Arg Tyr Ala Leu Glu Asn Gly Ala Ala Val Phe Ala Thr Glu 
        290                 295                 300             


Trp Gly Thr Ser Gln Ala Asn Gly Asp Gly Gly Pro Tyr Leu Asp Glu 
    305                 310                 315                 


Ala Asp Val Trp Leu Asn Phe Leu Asn Glu Asn Asn Ile Ser Trp Val 
320                 325                 330                 335 


Asn Trp Ser Leu Thr Asn Lys Asn Glu Thr Ser Gly Ser Phe Thr Pro 
                340                 345                 350     


Phe Glu Leu Gly Lys Ser Asn Ala Thr Ser Leu Asp Pro Gly Pro Glu 
            355                 360                 365         


Gln Ala Trp Ser Leu Pro Glu Leu Ser Val Ser Gly Glu Tyr Val Arg 
        370                 375                 380             


Ser Arg Ile Lys Gly Ser Pro Tyr Glu Pro Phe Asp Arg Thr Lys Phe 
    385                 390                 395                 


Asn Lys Val Ile Trp Asp Phe Asn Asp Gly Thr Val Gln Gly Phe Glu 
400                 405                 410                 415 


Val Asn Asp Asp Ser Pro Val Lys Glu Glu Ile Ala Val Ser Asn Ala 
                420                 425                 430     


Gly Asn Ala Leu Gln Ile Thr Gly Leu Asn Ala Ser Asn Asp Ile Ser 
            435                 440                 445         


Thr Asp Asn Phe Trp Ser Asn Leu Arg Leu Ser Ala Asn Ser Trp Gly 
        450                 455                 460             


Glu Ser Val Asn Ile Leu Gly Ala Glu Glu Leu Thr Leu Asp Val Ile 
    465                 470                 475                 


Val Asp Glu Pro Thr Ser Val Ser Ile Ala Ala Ile Pro Gln Ser Ala 
480                 485                 490                 495 


Ala Val Gly Trp Ala Asn Pro Asn Asn Ala Val Val Val Ser Lys Glu 
                500                 505                 510     


Asp Phe Ala Pro Tyr Gly Gly Gln Tyr Lys Ala Val Leu Thr Ile Thr 
            515                 520                 525         


Pro Glu Asp Ser Pro Ala Leu Gly Ala Ile Ala Thr His Ser Asp Asp 
        530                 535                 540             


Asn Met Met Asn Asn Ile Ile Leu Phe Ile Gly Thr Glu Asn Ala Asp 
    545                 550                 555                 


Val Leu Ser Leu Asp Asn Ile Thr Val Lys Gly Ser Ile Val Glu Ile 
560                 565                 570                 575 


Pro Val Ile His Asp Pro Lys Gly Ile Ala Val Leu Pro Ser Asn Phe 
                580                 585                 590     


Glu Asp Gly Thr Arg Gln Gly Trp Asp Trp Asn Pro Glu Ser Gly Val 
            595                 600                 605         


Lys Thr Ala Leu Thr Ile Lys Glu Ala Asp Gly Ser His Ala Leu Ser 
        610                 615                 620             


Trp Glu Phe Ala Tyr Pro Glu Val Lys Pro Gly Asp Gly Trp Ala Thr 
    625                 630                 635                 


Ala Pro Arg Leu Glu Phe Trp Lys Asp Gly Leu Val Arg Gly Ala Asn 
640                 645                 650                 655 


Asp Tyr Leu Ser Phe Asp Leu Tyr Leu Asp Pro Val Arg Ala Thr Glu 
                660                 665                 670     


Gly Ala Ile Thr Thr His Leu Val Phe Gln Pro Pro Ser Ala Gly Tyr 
            675                 680                 685         


Trp Val Gln Ala Pro Ala Ser His Ser Ile Asp Leu Leu Asn Leu Asp 
        690                 695                 700             


Ser Ala Asp Ile Thr Ala Asp Gly Leu Tyr His Tyr Glu Val Lys Phe 
    705                 710                 715                 


Asn Ile Arg Asp Ile Thr Ala Ile Gln Asp Asp Thr Ala Leu Arg Asn 
720                 725                 730                 735 


Met Ile Leu Ile Leu Glu Asp Arg Asn Ser Asp Phe Ala Gly Arg Ala 
                740                 745                 750     


Phe Ile Asp Asn Val Arg Phe Glu 
            755                 


<210>  3
<211>  32
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  3
gattaacgcg ttcctcgtgc tgagcacaga gg                                   32


<210>  4
<211>  31
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  4
ttatggagct caaatcaact ctaggaggct g                                    31


