                         SEQUENCE LISTING

<110>  Board of Regents, The University of Texas System,
 
<120>  THERAPIES FOR THE REGULATION OF INSULIN AND GLUCOSE USING RLIP76

<130>  124263-2015

<140>  PCT/US2006/042539
<141>  2007-02-07

<150>  10/714,506
<151>  2003-11-13

<150>  11/265,065
<151>  2005-11-02

<150>  10/713,578
<151>  2003-11-13

<150>  60/425,917
<151>  2002-11-13

<150>  60/425,814
<151>  2002-11-13

<160>  30    

<170>  PatentIn version 3.3

<210>  1
<211>  4368
<212>  DNA
<213>  homo sapiens


<220>
<221>  CDS
<222>  (216)..(2183)

<400>  1
atcattgtaa acaggcagag gctgggcggg gtgggaatgg ggcgcccgag gccggcctgg     60

ggcgcagcgc aggaggcggc tccggtggct gcggcggcag cgtgagcgcg aggaggcgga    120

ggctgcggcg gggcggacgg tcgcgcggcg gcaggcacag gtgtaatgga taggtaacag    180

agaagacctc gtcccttcct agtcagggca tcagc atg act gag tgc ttc ctg       233
                                       Met Thr Glu Cys Phe Leu          
                                       1               5                

ccc ccc acc agc agc ccc agt gaa cac cgc agg gtg gag cat ggc agc      281
Pro Pro Thr Ser Ser Pro Ser Glu His Arg Arg Val Glu His Gly Ser         
            10                  15                  20                  

ggg ctt acc cgg acc ccc agc tct gaa gag atc agc cct act aag ttt      329
Gly Leu Thr Arg Thr Pro Ser Ser Glu Glu Ile Ser Pro Thr Lys Phe         
        25                  30                  35                      

cct gga ttg tac cgc act ggc gag ccc tca cct ccc cat gac atc ctc      377
Pro Gly Leu Tyr Arg Thr Gly Glu Pro Ser Pro Pro His Asp Ile Leu         
    40                  45                  50                          

cat gag cct cct gat gta gtg tct gat gat gag aaa gat cat ggg aag      425
His Glu Pro Pro Asp Val Val Ser Asp Asp Glu Lys Asp His Gly Lys         
55                  60                  65                  70          

aaa aaa ggg aaa ttt aag aaa aag gaa aag agg act gaa ggc tat gca      473
Lys Lys Gly Lys Phe Lys Lys Lys Glu Lys Arg Thr Glu Gly Tyr Ala         
                75                  80                  85              

gcc ttt cag gaa gat agc tct gga gat gag gca gaa agt cct tct aaa      521
Ala Phe Gln Glu Asp Ser Ser Gly Asp Glu Ala Glu Ser Pro Ser Lys         
            90                  95                  100                 

atg aag agg tcc aag gga atc cat gtt ttc aag aag ccc agc ttt tct      569
Met Lys Arg Ser Lys Gly Ile His Val Phe Lys Lys Pro Ser Phe Ser         
        105                 110                 115                     

aaa aag aag gaa aag gat ttt aaa ata aaa gag aaa ccc aaa gaa gaa      617
Lys Lys Lys Glu Lys Asp Phe Lys Ile Lys Glu Lys Pro Lys Glu Glu         
    120                 125                 130                         

aag cat aaa gaa gaa aag cac aaa gaa gaa aaa cat aaa gag aag aag      665
Lys His Lys Glu Glu Lys His Lys Glu Glu Lys His Lys Glu Lys Lys         
135                 140                 145                 150         

tca aaa gac ttg aca gca gct gat gtt gtt aaa cag tgg aag gaa aag      713
Ser Lys Asp Leu Thr Ala Ala Asp Val Val Lys Gln Trp Lys Glu Lys         
                155                 160                 165             

aag aaa aag aaa aag cca att cag gag cca gag gtg cct cag att gat      761
Lys Lys Lys Lys Lys Pro Ile Gln Glu Pro Glu Val Pro Gln Ile Asp         
            170                 175                 180                 

gtt cca aat ctc aaa ccc att ttt gga att cct ttg gct gat gca gta      809
Val Pro Asn Leu Lys Pro Ile Phe Gly Ile Pro Leu Ala Asp Ala Val         
        185                 190                 195                     

gag agg acc atg atg tat gat ggc att cgg ctg cca gcc gtt ttc cgt      857
Glu Arg Thr Met Met Tyr Asp Gly Ile Arg Leu Pro Ala Val Phe Arg         
    200                 205                 210                         

gaa tgt ata gat tac gta gag aag tat ggc atg aag tgt gaa ggc atc      905
Glu Cys Ile Asp Tyr Val Glu Lys Tyr Gly Met Lys Cys Glu Gly Ile         
215                 220                 225                 230         

tac aga gta tca gga att aaa tca aag gtg gat gag cta aaa gca gcc      953
Tyr Arg Val Ser Gly Ile Lys Ser Lys Val Asp Glu Leu Lys Ala Ala         
                235                 240                 245             

tat gac cgg gag gag tct aca aac ttg gaa gac tat gag cct aac act     1001
Tyr Asp Arg Glu Glu Ser Thr Asn Leu Glu Asp Tyr Glu Pro Asn Thr         
            250                 255                 260                 

gta gcc agt ttg ctg aag cag tat ttg cga gac ctt cca gag aat ttg     1049
Val Ala Ser Leu Leu Lys Gln Tyr Leu Arg Asp Leu Pro Glu Asn Leu         
        265                 270                 275                     

ctt acc aaa gag ctt atg ccc aga ttt gaa gag gct tgt ggg agg acc     1097
Leu Thr Lys Glu Leu Met Pro Arg Phe Glu Glu Ala Cys Gly Arg Thr         
    280                 285                 290                         

acg gag act gag aaa gtg cag gaa ttc cag cgt tta ctc aaa gaa ctg     1145
Thr Glu Thr Glu Lys Val Gln Glu Phe Gln Arg Leu Leu Lys Glu Leu         
295                 300                 305                 310         

cca gaa tgt aac tat ctt ctg att tct tgg ctc att gtg cac atg gac     1193
Pro Glu Cys Asn Tyr Leu Leu Ile Ser Trp Leu Ile Val His Met Asp         
                315                 320                 325             

cat gtc att gca aag gaa ctg gaa aca aaa atg aat ata cag aac att     1241
His Val Ile Ala Lys Glu Leu Glu Thr Lys Met Asn Ile Gln Asn Ile         
            330                 335                 340                 

tct ata gtg ctc agc cca act gtg cag atc agc aat cga gtc ctg tat     1289
Ser Ile Val Leu Ser Pro Thr Val Gln Ile Ser Asn Arg Val Leu Tyr         
        345                 350                 355                     

gtg ttt ttc aca cat gtg caa gaa ctc ttt gga aat gtg gta cta aag     1337
Val Phe Phe Thr His Val Gln Glu Leu Phe Gly Asn Val Val Leu Lys         
    360                 365                 370                         

caa gtg atg aaa cct ctg cga tgg tct aac atg gcc acg atg ccc acg     1385
Gln Val Met Lys Pro Leu Arg Trp Ser Asn Met Ala Thr Met Pro Thr         
375                 380                 385                 390         

ctg cca gag acc cag gcg ggc atc aag gag gag atc agg aga cag gag     1433
Leu Pro Glu Thr Gln Ala Gly Ile Lys Glu Glu Ile Arg Arg Gln Glu         
                395                 400                 405             

ttt ctt ttg aat tgt tta cat cga gat ctg cag ggt ggg ata aag gat     1481
Phe Leu Leu Asn Cys Leu His Arg Asp Leu Gln Gly Gly Ile Lys Asp         
            410                 415                 420                 

ttg tct aaa gaa gaa aga tta tgg gaa gta caa aga att ttg aca gcc     1529
Leu Ser Lys Glu Glu Arg Leu Trp Glu Val Gln Arg Ile Leu Thr Ala         
        425                 430                 435                     

ctc aaa aga aaa ctg aga gaa gct aaa aga cag gag tgt gaa acc aag     1577
Leu Lys Arg Lys Leu Arg Glu Ala Lys Arg Gln Glu Cys Glu Thr Lys         
    440                 445                 450                         

att gca caa gag ata gcc agt ctt tca aaa gag gat gtt tcc aaa gaa     1625
Ile Ala Gln Glu Ile Ala Ser Leu Ser Lys Glu Asp Val Ser Lys Glu         
455                 460                 465                 470         

gag atg aat gaa aat gaa gaa gtt ata aat att ctc ctt gct cag gag     1673
Glu Met Asn Glu Asn Glu Glu Val Ile Asn Ile Leu Leu Ala Gln Glu         
                475                 480                 485             

aat gag atc ctg act gaa cag gag gag ctc ctg gcc atg gag cag ttt     1721
Asn Glu Ile Leu Thr Glu Gln Glu Glu Leu Leu Ala Met Glu Gln Phe         
            490                 495                 500                 

ctg cgc cgg cag att gcc tca gaa aaa gaa gag att gaa cgc ctc aga     1769
Leu Arg Arg Gln Ile Ala Ser Glu Lys Glu Glu Ile Glu Arg Leu Arg         
        505                 510                 515                     

gct gag att gct gaa att cag agt cgc cag cag cac ggc cga agt gag     1817
Ala Glu Ile Ala Glu Ile Gln Ser Arg Gln Gln His Gly Arg Ser Glu         
    520                 525                 530                         

act gag gag tac tcc tcc gag agc gag agc gag agt gag gat gag gag     1865
Thr Glu Glu Tyr Ser Ser Glu Ser Glu Ser Glu Ser Glu Asp Glu Glu         
535                 540                 545                 550         

gag ctg cag atc att ctg gaa gac tta cag aga cag aac gaa gag ctg     1913
Glu Leu Gln Ile Ile Leu Glu Asp Leu Gln Arg Gln Asn Glu Glu Leu         
                555                 560                 565             

gaa ata aag aac aat cat ttg aat caa gca att cat gag gag cgc gag     1961
Glu Ile Lys Asn Asn His Leu Asn Gln Ala Ile His Glu Glu Arg Glu         
            570                 575                 580                 

gcc atc atc gag ctg cgc gtg cag ctg cgg ctg ctc cag atg cag cga     2009
Ala Ile Ile Glu Leu Arg Val Gln Leu Arg Leu Leu Gln Met Gln Arg         
        585                 590                 595                     

gcc aag gcc gag cag cag gcg cag gag gac gag gag cct gag tgg cgc     2057
Ala Lys Ala Glu Gln Gln Ala Gln Glu Asp Glu Glu Pro Glu Trp Arg         
    600                 605                 610                         

ggg ggt gcc gtc cag ccg ccc aga gac ggc gtc ctt gag cca aaa gca     2105
Gly Gly Ala Val Gln Pro Pro Arg Asp Gly Val Leu Glu Pro Lys Ala         
615                 620                 625                 630         

gct aaa gag cag cca aag gca ggc aag gag ccg gca aag cca tcg ccc     2153
Ala Lys Glu Gln Pro Lys Ala Gly Lys Glu Pro Ala Lys Pro Ser Pro         
                635                 640                 645             

agc agg gat agg aag gag acg tcc atc tga gcagcctgcg tggccgtctg       2203
Ser Arg Asp Arg Lys Glu Thr Ser Ile                                     
            650                 655                                     

gagtccgtga gactgaaagg acccgtgcat cttactgtaa cccgggggcc aggccggctc   2263

tctcgctgta cattctgtaa aggtgtcttc tcttctcaga ctcttcctct gtcacacgtc   2323

tgactccttc acgtcaggct caggttccat gggaggacga agcagtggac gcattgtggg   2383

ctttagggac agatgagttt tccagatagt gtcagcttat ttgaagatta attttctttg   2443

ttaacttaaa ataactattt taacccttga gtggcttctt tttaaaccaa aaaccgtctt   2503

tctttgcttt tttatcacag cagaatcagg atctctttct cattcaaggg gggaaccacc   2563

ccaggtcagc gctgcgcctg ctgtggccgc cgcgagccac gccctctggg atctctggta   2623

ccgtcactct tgcttgtgcc ttccacacct tctcggtgca gatccctatg ggggagctgc   2683

ctcacgttct ctgactggtc agagcagcgc ctggtgggtg ttccctggcc cactctcctc   2743

tctccttctg cagttctaaa ccacagtcta taagcccgag tcaccaggac ggcctgtctg   2803

gccacagaca ggggctgcct gtggagcctg cccaccggcc cccggcagtg cagtccagcg   2863

gggaggaggc tgcccgttcc tgccagttcc tcactgcggg gaccagcaaa ggccttctca   2923

ctgggttggt caaaggtagt caccttggcc tggtgcatcc acagaggatg ttgttcaaac   2983

cagaaatctt ttaaacgact gaccttcctt aaaaacagaa tgactccgat tgcttgcttg   3043

ggctagaatg tacacgtctc cttgcctgaa taagccatat atatgctctt aaacaaaagt   3103

ttgaaattat ccatatcatc tcagtgaacc tactggtgga ctcccaattg acaagattga   3163

gcaatagaaa aaaattcctt tcctttgaat gatagctgtg attcacccca ccccattttc   3223

ttgtttctgg tccatccgat gagacggatg ctctgatgct ctgaggcttc tgggaggctg   3283

ggccctggag gcaacgtgct gcaggcgcac tctgtcagag tgaacagcac cgcgagacag   3343

gccaggctcg tggctcggaa gacaaacccc acacacactc aaggggtcga aaacaaaccc   3403

cacacgaggg ctctcacctc cttctcctag gtagtattta ttttcagcac ctgtttgatg   3463

cagtttttaa tcctctacct attgcactgt tgtgactcgt tggccattat ttgatttttg   3523

tacgaaaaaa agctttgtta tagaaatcag catactattt ttttaaatct ggagagaaga   3583

tattctggtg actgaaagta tggtcgggtg tcagatataa atgtgcaaat gccttcttgc   3643

tgtcctgtcg gtctcagtac gttcacttta tagctgctgg caatatcgaa ggttcctttt   3703

ttgtttgtgt aaactctaat ttctatcaag gtgtcatgga tttttaaaat tagtatttca   3763

ttacaaatgt ctcagcattg gttaactaat ttttgccagg accattattg atcaagcaaa   3823

taaattcaac agccatttgg gaaaaagaaa agcttctagt ttttttgtac acattctttc   3883

tgtgaggaga ttgagtactc tgcagctggc gaggagttgg ttgaggcact tcttcaaggc   3943

caagggggaa cacagtgttt tgtttccagc tcactttgta cccctcacct ctgcagacac   4003

ggggagaacc ccggacccct ggcatgcatg ctggcggcgg catgcctccc ttccacaagc   4063

ccatgctgct gcagagggag cctgtgtttg caaaacccag tggactgggc tgggtctgct   4123

gtctgagcag ctcctggctc cggtgggaac tgcacacaag tccactggcc tggcttggcc   4183

ccaggcattg caattgacag acatttgcat ttcatacggt aaatgaggac tcagcacagc   4243

caaccataat cagcatgtct gggatagact ggtctagaat aaaaatgaag tttccattgc   4303

tttgtttgct ttaaaaattc cacaattaaa atatctgtca ttgaaagctt aaaaaaaaaa   4363

aaaaa                                                               4368


<210>  2
<211>  655
<212>  PRT
<213>  homo sapiens

<400>  2

Met Thr Glu Cys Phe Leu Pro Pro Thr Ser Ser Pro Ser Glu His Arg 
1               5                   10                  15      


Arg Val Glu His Gly Ser Gly Leu Thr Arg Thr Pro Ser Ser Glu Glu 
            20                  25                  30          


Ile Ser Pro Thr Lys Phe Pro Gly Leu Tyr Arg Thr Gly Glu Pro Ser 
        35                  40                  45              


Pro Pro His Asp Ile Leu His Glu Pro Pro Asp Val Val Ser Asp Asp 
    50                  55                  60                  


Glu Lys Asp His Gly Lys Lys Lys Gly Lys Phe Lys Lys Lys Glu Lys 
65                  70                  75                  80  


Arg Thr Glu Gly Tyr Ala Ala Phe Gln Glu Asp Ser Ser Gly Asp Glu 
                85                  90                  95      


Ala Glu Ser Pro Ser Lys Met Lys Arg Ser Lys Gly Ile His Val Phe 
            100                 105                 110         


Lys Lys Pro Ser Phe Ser Lys Lys Lys Glu Lys Asp Phe Lys Ile Lys 
        115                 120                 125             


Glu Lys Pro Lys Glu Glu Lys His Lys Glu Glu Lys His Lys Glu Glu 
    130                 135                 140                 


Lys His Lys Glu Lys Lys Ser Lys Asp Leu Thr Ala Ala Asp Val Val 
145                 150                 155                 160 


Lys Gln Trp Lys Glu Lys Lys Lys Lys Lys Lys Pro Ile Gln Glu Pro 
                165                 170                 175     


Glu Val Pro Gln Ile Asp Val Pro Asn Leu Lys Pro Ile Phe Gly Ile 
            180                 185                 190         


Pro Leu Ala Asp Ala Val Glu Arg Thr Met Met Tyr Asp Gly Ile Arg 
        195                 200                 205             


Leu Pro Ala Val Phe Arg Glu Cys Ile Asp Tyr Val Glu Lys Tyr Gly 
    210                 215                 220                 


Met Lys Cys Glu Gly Ile Tyr Arg Val Ser Gly Ile Lys Ser Lys Val 
225                 230                 235                 240 


Asp Glu Leu Lys Ala Ala Tyr Asp Arg Glu Glu Ser Thr Asn Leu Glu 
                245                 250                 255     


Asp Tyr Glu Pro Asn Thr Val Ala Ser Leu Leu Lys Gln Tyr Leu Arg 
            260                 265                 270         


Asp Leu Pro Glu Asn Leu Leu Thr Lys Glu Leu Met Pro Arg Phe Glu 
        275                 280                 285             


Glu Ala Cys Gly Arg Thr Thr Glu Thr Glu Lys Val Gln Glu Phe Gln 
    290                 295                 300                 


Arg Leu Leu Lys Glu Leu Pro Glu Cys Asn Tyr Leu Leu Ile Ser Trp 
305                 310                 315                 320 


Leu Ile Val His Met Asp His Val Ile Ala Lys Glu Leu Glu Thr Lys 
                325                 330                 335     


Met Asn Ile Gln Asn Ile Ser Ile Val Leu Ser Pro Thr Val Gln Ile 
            340                 345                 350         


Ser Asn Arg Val Leu Tyr Val Phe Phe Thr His Val Gln Glu Leu Phe 
        355                 360                 365             


Gly Asn Val Val Leu Lys Gln Val Met Lys Pro Leu Arg Trp Ser Asn 
    370                 375                 380                 


Met Ala Thr Met Pro Thr Leu Pro Glu Thr Gln Ala Gly Ile Lys Glu 
385                 390                 395                 400 


Glu Ile Arg Arg Gln Glu Phe Leu Leu Asn Cys Leu His Arg Asp Leu 
                405                 410                 415     


Gln Gly Gly Ile Lys Asp Leu Ser Lys Glu Glu Arg Leu Trp Glu Val 
            420                 425                 430         


Gln Arg Ile Leu Thr Ala Leu Lys Arg Lys Leu Arg Glu Ala Lys Arg 
        435                 440                 445             


Gln Glu Cys Glu Thr Lys Ile Ala Gln Glu Ile Ala Ser Leu Ser Lys 
    450                 455                 460                 


Glu Asp Val Ser Lys Glu Glu Met Asn Glu Asn Glu Glu Val Ile Asn 
465                 470                 475                 480 


Ile Leu Leu Ala Gln Glu Asn Glu Ile Leu Thr Glu Gln Glu Glu Leu 
                485                 490                 495     


Leu Ala Met Glu Gln Phe Leu Arg Arg Gln Ile Ala Ser Glu Lys Glu 
            500                 505                 510         


Glu Ile Glu Arg Leu Arg Ala Glu Ile Ala Glu Ile Gln Ser Arg Gln 
        515                 520                 525             


Gln His Gly Arg Ser Glu Thr Glu Glu Tyr Ser Ser Glu Ser Glu Ser 
    530                 535                 540                 


Glu Ser Glu Asp Glu Glu Glu Leu Gln Ile Ile Leu Glu Asp Leu Gln 
545                 550                 555                 560 


Arg Gln Asn Glu Glu Leu Glu Ile Lys Asn Asn His Leu Asn Gln Ala 
                565                 570                 575     


Ile His Glu Glu Arg Glu Ala Ile Ile Glu Leu Arg Val Gln Leu Arg 
            580                 585                 590         


Leu Leu Gln Met Gln Arg Ala Lys Ala Glu Gln Gln Ala Gln Glu Asp 
        595                 600                 605             


Glu Glu Pro Glu Trp Arg Gly Gly Ala Val Gln Pro Pro Arg Asp Gly 
    610                 615                 620                 


Val Leu Glu Pro Lys Ala Ala Lys Glu Gln Pro Lys Ala Gly Lys Glu 
625                 630                 635                 640 


Pro Ala Lys Pro Ser Pro Ser Arg Asp Arg Lys Glu Thr Ser Ile 
                645                 650                 655 


<210>  3
<211>  66
<212>  PRT
<213>  artificial sequence

<220>
<223>  epitope region comprising residues 154-219

<400>  3

Leu Thr Ala Ala Asp Val Val Lys Gln Trp Lys Glu Lys Lys Lys Lys 
1               5                   10                  15      


Lys Lys Pro Ile Gln Glu Pro Glu Val Pro Gln Ile Asp Val Pro Asn 
            20                  25                  30          


Leu Lys Pro Ile Phe Gly Ile Pro Leu Ala Asp Ala Val Glu Arg Thr 
        35                  40                  45              


Met Met Tyr Asp Gly Ile Arg Leu Pro Ala Val Phe Arg Glu Cys Ile 
    50                  55                  60                  


Asp Tyr 
65      


<210>  4
<211>  15
<212>  PRT
<213>  artificial sequence

<220>
<223>  epitope region comprising residues 171-185

<400>  4

Lys Pro Ile Gln Glu Pro Glu Val Pro Gln Ile Asp Val Pro Asn 
1               5                   10                  15  


<210>  5
<211>  26
<212>  DNA
<213>  artificial sequence

<220>
<223>  upstream primer sequence for clone of full length RLIP

<400>  5
ggcggatcca tgactgagtg cttcct                                          26


<210>  6
<211>  34
<212>  DNA
<213>  artificial sequence

<220>
<223>  downstream primer sequence for clone of full length RLIP

<400>  6
ccgctcgagt agatggacgt ctccttccta tccc                                 34


<210>  7
<211>  30
<212>  DNA
<213>  artificial sequence

<220>
<223>  mutagenic upstream primer for deletion mutant 203-219

<400>  7
gtagagagga ccatggtaga gaagtatggc                                      30


<210>  8
<211>  31
<212>  DNA
<213>  artificial sequence

<220>
<223>  mutagenic upstream primer for deletion mutant 154-171

<400>  8
gaagaagtca aaagacaagc caattcagga g                                    31


<210>  9
<211>  28
<212>  DNA
<213>  artificial sequence

<220>
<223>  mutagenic upstream primer for deletion mutant 171-185

<400>  9
gaagaaaaag aaactcaaac ccattttt                                        28


<210>  10
<211>  31
<212>  DNA
<213>  artificial sequence

<220>
<223>  mutagenic upstream primer for deletion mutant 154-219

<400>  10
gaagaagtca aaagacgtag agaagtatgg c                                    31


<210>  11
<211>  31
<212>  DNA
<213>  artificial sequence

<220>
<223>  mutagenic upstream primer for deletion mutant 415-448

<400>  11
gaattgttta catcgacagg agtgtgaaac c                                    31


<210>  12
<211>  28
<212>  DNA
<213>  artificial sequence

<220>
<223>  mutagenic upstream primer for deletion mutant 65-80

<400>  12
gtgtctgatg ataggactga aggctatg                                        28


<210>  13
<211>  23
<212>  DNA
<213>  artificial sequence

<220>
<223>  targeted cDNA corresponding to nucleotides 508-528 of ral A 
       binding protein 1

<400>  13
aagaaaaagc caattcagga gcc                                             23


<210>  14
<211>  23
<212>  RNA
<213>  artificial sequence

<220>
<223>  corresponding sense siRNA strand for targeted cDNA


<220>
<221>  d
<222>  (22)..(23)
<223>  dT

<400>  14
gaaaaagcca auucaggagc cdd                                             23


<210>  15
<211>  23
<212>  RNA
<213>  artificial sequence

<220>
<223>  corresponding antisense siRNA strand for targeted cDNA


<220>
<221>  d
<222>  (22)..(23)
<223>  dT

<400>  15
ggcuccugaa uuggcuuuuu cdd                                             23


<210>  16
<211>  23
<212>  RNA
<213>  artificial sequence

<220>
<223>  scrambled sense siRNA strand


<220>
<221>  d
<222>  (22)..(23)
<223>  dT

<400>  16
guaacugcaa cgauuucgau gdd                                             23


<210>  17
<211>  23
<212>  RNA
<213>  artificial sequence

<220>
<223>  scrambled antisense siRNA strand


<220>
<221>  d
<222>  (22)..(23)
<223>  dT

<400>  17
caucgaaauc guugcaguua cdd                                             23


<210>  18
<211>  21
<212>  DNA
<213>  artificial sequence

<220>
<223>  phosphorothioate antisense oligonucleotides

<400>  18
ggctcctgaa ttggcttttt c                                               21


<210>  19
<211>  22
<212>  DNA
<213>  artificial sequence

<220>
<223>  corresponding silencing RNA sequence to the phosphorothioate 
       antisense oligonucleotide

<400>  19
aagaaaagcc aattcaggag cc                                              22


<210>  20
<211>  198
<212>  DNA
<213>  artificial sequence

<220>
<223>  epitope nucleotide sequence corresponding to residues 154-219

<400>  20
ttgacagcag ctgatgttgt taaacagtgg aaggaaaaga agaaaaagaa aaagccaatt     60

caggagccag aggtgcctca gattgatgtt ccaaatctca aacccatttt tggaattcct    120

ttggctgatg cagtagagag gaccatgatg tatgatggca ttcggctgcc agccgttttc    180

cgtgaatgta tagattac                                                  198


<210>  21
<211>  45
<212>  DNA
<213>  artificial sequence

<220>
<223>  epitope nucleotide sequence corresponding to residues 171-185

<400>  21
aagccaattc aggagccaga ggtgcctcag attgatgttc caaat                     45


<210>  22
<211>  1968
<212>  DNA
<213>  homo sapiens


<220>
<221>  CDS
<222>  (1)..(1965)

<400>  22
atg act gag tgc ttc ctg ccc ccc acc agc agc ccc agt gaa cac cgc       48
Met Thr Glu Cys Phe Leu Pro Pro Thr Ser Ser Pro Ser Glu His Arg         
1               5                   10                  15              

agg gtg gag cat ggc agc ggg ctt acc cgg acc ccc agc tct gaa gag       96
Arg Val Glu His Gly Ser Gly Leu Thr Arg Thr Pro Ser Ser Glu Glu         
            20                  25                  30                  

atc agc cct act aag ttt cct gga ttg tac cgc act ggc gag ccc tca      144
Ile Ser Pro Thr Lys Phe Pro Gly Leu Tyr Arg Thr Gly Glu Pro Ser         
        35                  40                  45                      

cct ccc cat gac atc ctc cat gag cct cct gat gta gtg tct gat gat      192
Pro Pro His Asp Ile Leu His Glu Pro Pro Asp Val Val Ser Asp Asp         
    50                  55                  60                          

gag aaa gat cat ggg aag aaa aaa ggg aaa ttt aag aaa aag gaa aag      240
Glu Lys Asp His Gly Lys Lys Lys Gly Lys Phe Lys Lys Lys Glu Lys         
65                  70                  75                  80          

agg act gaa ggc tat gca gcc ttt cag gaa gat agc tct gga gat gag      288
Arg Thr Glu Gly Tyr Ala Ala Phe Gln Glu Asp Ser Ser Gly Asp Glu         
                85                  90                  95              

gca gaa agt cct tct aaa atg aag agg tcc aag gga atc cat gtt ttc      336
Ala Glu Ser Pro Ser Lys Met Lys Arg Ser Lys Gly Ile His Val Phe         
            100                 105                 110                 

aag aag ccc agc ttt tct aaa aag aag gaa aag gat ttt aaa ata aaa      384
Lys Lys Pro Ser Phe Ser Lys Lys Lys Glu Lys Asp Phe Lys Ile Lys         
        115                 120                 125                     

gag aaa ccc aaa gaa gaa aag cat aaa gaa gaa aag cac aaa gaa gaa      432
Glu Lys Pro Lys Glu Glu Lys His Lys Glu Glu Lys His Lys Glu Glu         
    130                 135                 140                         

aaa cat aaa gag aag aag tca aaa gac ttg aca gca gct gat gtt gtt      480
Lys His Lys Glu Lys Lys Ser Lys Asp Leu Thr Ala Ala Asp Val Val         
145                 150                 155                 160         

aaa cag tgg aag gaa aag aag aaa aag aaa aag cca att cag gag cca      528
Lys Gln Trp Lys Glu Lys Lys Lys Lys Lys Lys Pro Ile Gln Glu Pro         
                165                 170                 175             

gag gtg cct cag att gat gtt cca aat ctc aaa ccc att ttt gga att      576
Glu Val Pro Gln Ile Asp Val Pro Asn Leu Lys Pro Ile Phe Gly Ile         
            180                 185                 190                 

cct ttg gct gat gca gta gag agg acc atg atg tat gat ggc att cgg      624
Pro Leu Ala Asp Ala Val Glu Arg Thr Met Met Tyr Asp Gly Ile Arg         
        195                 200                 205                     

ctg cca gcc gtt ttc cgt gaa tgt ata gat tac gta gag aag tat ggc      672
Leu Pro Ala Val Phe Arg Glu Cys Ile Asp Tyr Val Glu Lys Tyr Gly         
    210                 215                 220                         

atg aag tgt gaa ggc atc tac aga gta tca gga att aaa tca aag gtg      720
Met Lys Cys Glu Gly Ile Tyr Arg Val Ser Gly Ile Lys Ser Lys Val         
225                 230                 235                 240         

gat gag cta aaa gca gcc tat gac cgg gag gag tct aca aac ttg gaa      768
Asp Glu Leu Lys Ala Ala Tyr Asp Arg Glu Glu Ser Thr Asn Leu Glu         
                245                 250                 255             

gac tat gag cct aac act gta gcc agt ttg ctg aag cag tat ttg cga      816
Asp Tyr Glu Pro Asn Thr Val Ala Ser Leu Leu Lys Gln Tyr Leu Arg         
            260                 265                 270                 

gac ctt cca gag aat ttg ctt acc aaa gag ctt atg ccc aga ttt gaa      864
Asp Leu Pro Glu Asn Leu Leu Thr Lys Glu Leu Met Pro Arg Phe Glu         
        275                 280                 285                     

gag gct tgt ggg agg acc acg gag act gag aaa gtg cag gaa ttc cag      912
Glu Ala Cys Gly Arg Thr Thr Glu Thr Glu Lys Val Gln Glu Phe Gln         
    290                 295                 300                         

cgt tta ctc aaa gaa ctg cca gaa tgt aac tat ctt ctg att tct tgg      960
Arg Leu Leu Lys Glu Leu Pro Glu Cys Asn Tyr Leu Leu Ile Ser Trp         
305                 310                 315                 320         

ctc att gtg cac atg gac cat gtc att gca aag gaa ctg gaa aca aaa     1008
Leu Ile Val His Met Asp His Val Ile Ala Lys Glu Leu Glu Thr Lys         
                325                 330                 335             

atg aat ata cag aac att tct ata gtg ctc agc cca act gtg cag atc     1056
Met Asn Ile Gln Asn Ile Ser Ile Val Leu Ser Pro Thr Val Gln Ile         
            340                 345                 350                 

agc aat cga gtc ctg tat gtg ttt ttc aca cat gtg caa gaa ctc ttt     1104
Ser Asn Arg Val Leu Tyr Val Phe Phe Thr His Val Gln Glu Leu Phe         
        355                 360                 365                     

gga aat gtg gta cta aag caa gtg atg aaa cct ctg cga tgg tct aac     1152
Gly Asn Val Val Leu Lys Gln Val Met Lys Pro Leu Arg Trp Ser Asn         
    370                 375                 380                         

atg gcc acg atg ccc acg ctg cca gag acc cag gcg ggc atc aag gag     1200
Met Ala Thr Met Pro Thr Leu Pro Glu Thr Gln Ala Gly Ile Lys Glu         
385                 390                 395                 400         

gag atc agg aga cag gag ttt ctt ttg aat tgt tta cat cga gat ctg     1248
Glu Ile Arg Arg Gln Glu Phe Leu Leu Asn Cys Leu His Arg Asp Leu         
                405                 410                 415             

cag ggt ggg ata aag gat ttg tct aaa gaa gaa aga tta tgg gaa gta     1296
Gln Gly Gly Ile Lys Asp Leu Ser Lys Glu Glu Arg Leu Trp Glu Val         
            420                 425                 430                 

caa aga att ttg aca gcc ctc aaa aga aaa ctg aga gaa gct aaa aga     1344
Gln Arg Ile Leu Thr Ala Leu Lys Arg Lys Leu Arg Glu Ala Lys Arg         
        435                 440                 445                     

cag gag tgt gaa acc aag att gca caa gag ata gcc agt ctt tca aaa     1392
Gln Glu Cys Glu Thr Lys Ile Ala Gln Glu Ile Ala Ser Leu Ser Lys         
    450                 455                 460                         

gag gat gtt tcc aaa gaa gag atg aat gaa aat gaa gaa gtt ata aat     1440
Glu Asp Val Ser Lys Glu Glu Met Asn Glu Asn Glu Glu Val Ile Asn         
465                 470                 475                 480         

att ctc ctt gct cag gag aat gag atc ctg act gaa cag gag gag ctc     1488
Ile Leu Leu Ala Gln Glu Asn Glu Ile Leu Thr Glu Gln Glu Glu Leu         
                485                 490                 495             

ctg gcc atg gag cag ttt ctg cgc cgg cag att gcc tca gaa aaa gaa     1536
Leu Ala Met Glu Gln Phe Leu Arg Arg Gln Ile Ala Ser Glu Lys Glu         
            500                 505                 510                 

gag att gaa cgc ctc aga gct gag att gct gaa att cag agt cgc cag     1584
Glu Ile Glu Arg Leu Arg Ala Glu Ile Ala Glu Ile Gln Ser Arg Gln         
        515                 520                 525                     

cag cac ggc cga agt gag act gag gag tac tcc tcc gag agc gag agc     1632
Gln His Gly Arg Ser Glu Thr Glu Glu Tyr Ser Ser Glu Ser Glu Ser         
    530                 535                 540                         

gag agt gag gat gag gag gag ctg cag atc att ctg gaa gac tta cag     1680
Glu Ser Glu Asp Glu Glu Glu Leu Gln Ile Ile Leu Glu Asp Leu Gln         
545                 550                 555                 560         

aga cag aac gaa gag ctg gaa ata aag aac aat cat ttg aat caa gca     1728
Arg Gln Asn Glu Glu Leu Glu Ile Lys Asn Asn His Leu Asn Gln Ala         
                565                 570                 575             

att cat gag gag cgc gag gcc atc atc gag ctg cgc gtg cag ctg cgg     1776
Ile His Glu Glu Arg Glu Ala Ile Ile Glu Leu Arg Val Gln Leu Arg         
            580                 585                 590                 

ctg ctc cag atg cag cga gcc aag gcc gag cag cag gcg cag gag gac     1824
Leu Leu Gln Met Gln Arg Ala Lys Ala Glu Gln Gln Ala Gln Glu Asp         
        595                 600                 605                     

gag gag cct gag tgg cgc ggg ggt gcc gtc cag ccg ccc aga gac ggc     1872
Glu Glu Pro Glu Trp Arg Gly Gly Ala Val Gln Pro Pro Arg Asp Gly         
    610                 615                 620                         

gtc ctt gag cca aaa gca gct aaa gag cag cca aag gca ggc aag gag     1920
Val Leu Glu Pro Lys Ala Ala Lys Glu Gln Pro Lys Ala Gly Lys Glu         
625                 630                 635                 640         

ccg gca aag cca tcg ccc agc agg gat agg aag gag acg tcc atc tga     1968
Pro Ala Lys Pro Ser Pro Ser Arg Asp Arg Lys Glu Thr Ser Ile             
                645                 650                 655             


<210>  23
<211>  655
<212>  PRT
<213>  homo sapiens

<400>  23

Met Thr Glu Cys Phe Leu Pro Pro Thr Ser Ser Pro Ser Glu His Arg 
1               5                   10                  15      


Arg Val Glu His Gly Ser Gly Leu Thr Arg Thr Pro Ser Ser Glu Glu 
            20                  25                  30          


Ile Ser Pro Thr Lys Phe Pro Gly Leu Tyr Arg Thr Gly Glu Pro Ser 
        35                  40                  45              


Pro Pro His Asp Ile Leu His Glu Pro Pro Asp Val Val Ser Asp Asp 
    50                  55                  60                  


Glu Lys Asp His Gly Lys Lys Lys Gly Lys Phe Lys Lys Lys Glu Lys 
65                  70                  75                  80  


Arg Thr Glu Gly Tyr Ala Ala Phe Gln Glu Asp Ser Ser Gly Asp Glu 
                85                  90                  95      


Ala Glu Ser Pro Ser Lys Met Lys Arg Ser Lys Gly Ile His Val Phe 
            100                 105                 110         


Lys Lys Pro Ser Phe Ser Lys Lys Lys Glu Lys Asp Phe Lys Ile Lys 
        115                 120                 125             


Glu Lys Pro Lys Glu Glu Lys His Lys Glu Glu Lys His Lys Glu Glu 
    130                 135                 140                 


Lys His Lys Glu Lys Lys Ser Lys Asp Leu Thr Ala Ala Asp Val Val 
145                 150                 155                 160 


Lys Gln Trp Lys Glu Lys Lys Lys Lys Lys Lys Pro Ile Gln Glu Pro 
                165                 170                 175     


Glu Val Pro Gln Ile Asp Val Pro Asn Leu Lys Pro Ile Phe Gly Ile 
            180                 185                 190         


Pro Leu Ala Asp Ala Val Glu Arg Thr Met Met Tyr Asp Gly Ile Arg 
        195                 200                 205             


Leu Pro Ala Val Phe Arg Glu Cys Ile Asp Tyr Val Glu Lys Tyr Gly 
    210                 215                 220                 


Met Lys Cys Glu Gly Ile Tyr Arg Val Ser Gly Ile Lys Ser Lys Val 
225                 230                 235                 240 


Asp Glu Leu Lys Ala Ala Tyr Asp Arg Glu Glu Ser Thr Asn Leu Glu 
                245                 250                 255     


Asp Tyr Glu Pro Asn Thr Val Ala Ser Leu Leu Lys Gln Tyr Leu Arg 
            260                 265                 270         


Asp Leu Pro Glu Asn Leu Leu Thr Lys Glu Leu Met Pro Arg Phe Glu 
        275                 280                 285             


Glu Ala Cys Gly Arg Thr Thr Glu Thr Glu Lys Val Gln Glu Phe Gln 
    290                 295                 300                 


Arg Leu Leu Lys Glu Leu Pro Glu Cys Asn Tyr Leu Leu Ile Ser Trp 
305                 310                 315                 320 


Leu Ile Val His Met Asp His Val Ile Ala Lys Glu Leu Glu Thr Lys 
                325                 330                 335     


Met Asn Ile Gln Asn Ile Ser Ile Val Leu Ser Pro Thr Val Gln Ile 
            340                 345                 350         


Ser Asn Arg Val Leu Tyr Val Phe Phe Thr His Val Gln Glu Leu Phe 
        355                 360                 365             


Gly Asn Val Val Leu Lys Gln Val Met Lys Pro Leu Arg Trp Ser Asn 
    370                 375                 380                 


Met Ala Thr Met Pro Thr Leu Pro Glu Thr Gln Ala Gly Ile Lys Glu 
385                 390                 395                 400 


Glu Ile Arg Arg Gln Glu Phe Leu Leu Asn Cys Leu His Arg Asp Leu 
                405                 410                 415     


Gln Gly Gly Ile Lys Asp Leu Ser Lys Glu Glu Arg Leu Trp Glu Val 
            420                 425                 430         


Gln Arg Ile Leu Thr Ala Leu Lys Arg Lys Leu Arg Glu Ala Lys Arg 
        435                 440                 445             


Gln Glu Cys Glu Thr Lys Ile Ala Gln Glu Ile Ala Ser Leu Ser Lys 
    450                 455                 460                 


Glu Asp Val Ser Lys Glu Glu Met Asn Glu Asn Glu Glu Val Ile Asn 
465                 470                 475                 480 


Ile Leu Leu Ala Gln Glu Asn Glu Ile Leu Thr Glu Gln Glu Glu Leu 
                485                 490                 495     


Leu Ala Met Glu Gln Phe Leu Arg Arg Gln Ile Ala Ser Glu Lys Glu 
            500                 505                 510         


Glu Ile Glu Arg Leu Arg Ala Glu Ile Ala Glu Ile Gln Ser Arg Gln 
        515                 520                 525             


Gln His Gly Arg Ser Glu Thr Glu Glu Tyr Ser Ser Glu Ser Glu Ser 
    530                 535                 540                 


Glu Ser Glu Asp Glu Glu Glu Leu Gln Ile Ile Leu Glu Asp Leu Gln 
545                 550                 555                 560 


Arg Gln Asn Glu Glu Leu Glu Ile Lys Asn Asn His Leu Asn Gln Ala 
                565                 570                 575     


Ile His Glu Glu Arg Glu Ala Ile Ile Glu Leu Arg Val Gln Leu Arg 
            580                 585                 590         


Leu Leu Gln Met Gln Arg Ala Lys Ala Glu Gln Gln Ala Gln Glu Asp 
        595                 600                 605             


Glu Glu Pro Glu Trp Arg Gly Gly Ala Val Gln Pro Pro Arg Asp Gly 
    610                 615                 620                 


Val Leu Glu Pro Lys Ala Ala Lys Glu Gln Pro Lys Ala Gly Lys Glu 
625                 630                 635                 640 


Pro Ala Lys Pro Ser Pro Ser Arg Asp Arg Lys Glu Thr Ser Ile 
                645                 650                 655 


<210>  24
<211>  1980
<212>  DNA
<213>  artificial sequence

<220>
<223>  POB1 cDNA

<400>  24
atggaggcgg cagcggcggc ggcggcggcg gcagcggcag cggcagcggc gggcgggggc     60

tgtggctccg ggccgccgcc gctgctgctg agcgagggcg agcagcagtg ctactccgag    120

ctcttcgcgc gctgtgccgg cgccgcgggc gggggccccg ggtctgggcc ccccgaggcc    180

gccagagtcg cccccggcac ggccactgcg gccgccggcc ccgtggctga cctgtttcgg    240

gcatcgcagc tgcccgccga gacgctgcac cagatcacag aactgtgtgg tgcaaagcgg    300

gttggttatt ttggtccaac acagttttac attgccctga aattaattgc tgcagcacaa    360

tctggcctcc cggtacggat agagagtatt aaatgtgaat tgcctctgcc tcgctttatg    420

atgtcaaaga atgatggtga gatacgattt gggaacccag ctgagctgca tggaactaag    480

gttcagattc catatttaac tacagaaaaa aattccttca aaagaatgga cgatgaggat    540

aaacaggaaa cacagtctcc cacgatgtca cccctcgcct cccctccttc ttccccgcct    600

cattaccaga gggtgccctt gagccatggc tacagcaaac tgcggagcag cgcagaacag    660

atgcatccag caccttatga agctaggcag ccccttgtcc agcccgaggg atcctcatca    720

gggggcccag gaaccaagcc ccttcggcat caggcttccc ttatccggtc cttttcagtg    780

gagagggaac tacaggataa cagcagttac cccgacgaac cctggaggat aacagaagaa    840

cagcgcgagt actatgtcaa tcagttccga tcccttcagc cagacccaag ctctttcatt    900

tcaggttctg tggccaagaa cttcttcacc aaatcaaagc tttccattcc agaactctcc    960

tatatatggg agcttagtga tgctgactgt gatggagccc tgaccctgcc tgagttctgt   1020

gctgcgtttc atctcattgt ggctcggaag aacggctacc cattgcctga gggcctccct   1080

ccaactctgc agccagaata cctgcaggca gcttttccta agcccaaatg ggactgtcaa   1140

ttatttgatt cttattctga gtcactgccg gcaaatcaac aacctcgtga cttgaatcgg   1200

atggagaaga catctgttaa agacatggct gaccttcctg tccctaacca ggatgtaact   1260

agtgatgaca aacaagcttt gaaaagtact atcaatgaag ccttaccaaa ggacgtgtct   1320

gaggatccag caactcccaa ggattccaac agtctcaaag caagaccaag atccagatct   1380

tactctagca cctccataga agaggccatg aaaaggggcg aggaccctcc caccccgcca   1440

cctcggccac agaaaaccca ttccagagcc tcctccttgg atctgaataa agtcttccag   1500

cccagtgtgc cagctaccaa gtcaggattg ttacccccac cacctgcgct ccctccaaga   1560

ccttgtccat cacagtctga acaagtgtcg gaggccgagt tactcccaca gctgagcaga   1620

gccccatccc aggctgcaga aagtagtcca gcaaagaagg atgtactgta ttctcagcca   1680

ccatcaaagc ccattcgtag gaaattcaga ccagaaaacc aagctacaga aaaccaagag   1740

ccttccactg ctgcaagtgg gccagcttct gcggcaacca tgaaaccgca tccaacagtc   1800

caaaagcagt cttccaaaca gaagaaggcc attcaaactg ctatccgcaa aaataaagag   1860

gcaaacgcag tgctggctcg gctgaacagt gagctccagc agcagctcaa ggaggttcat   1920

caagaacgaa ttgcattgga aaaccaattg gaacaacttc gtccggtcac tgtgttgtga   1980


<210>  25
<211>  26
<212>  DNA
<213>  artificial sequence

<220>
<223>  upstream primer for POB1

<400>  25
ggcggatcca tggaggcggc agcggc                                          26


<210>  26
<211>  29
<212>  DNA
<213>  artificial sequence

<220>
<223>  downstream primer for POB1

<400>  26
ccgctcgagt cacaacacag tgaccggac                                       29


<210>  27
<211>  512
<212>  PRT
<213>  artificial sequence

<220>
<223>  POB1 deletion mutant: 1-512 residues

<400>  27

Met Glu Ala Ala Ala Ala Ala Ala Ala Ala Ala Ala Ala Ala Ala Ala 
1               5                   10                  15      


Ala Gly Gly Gly Cys Gly Ser Gly Pro Pro Pro Leu Leu Leu Ser Glu 
            20                  25                  30          


Gly Glu Gln Gln Cys Tyr Ser Glu Leu Phe Ala Arg Cys Ala Gly Ala 
        35                  40                  45              


Ala Gly Gly Gly Pro Gly Ser Gly Pro Pro Glu Ala Ala Arg Val Ala 
    50                  55                  60                  


Pro Gly Thr Ala Thr Ala Ala Ala Gly Pro Val Ala Asp Leu Phe Arg 
65                  70                  75                  80  


Ala Ser Gln Leu Pro Ala Glu Thr Leu His Gln Ile Thr Glu Leu Cys 
                85                  90                  95      


Gly Ala Lys Arg Val Gly Tyr Phe Gly Pro Thr Gln Phe Tyr Ile Ala 
            100                 105                 110         


Leu Lys Leu Ile Ala Ala Ala Gln Ser Gly Leu Pro Val Arg Ile Glu 
        115                 120                 125             


Ser Ile Lys Cys Glu Leu Pro Leu Pro Arg Phe Met Met Ser Lys Asn 
    130                 135                 140                 


Asp Gly Glu Ile Arg Phe Gly Asn Pro Ala Glu Leu His Gly Thr Lys 
145                 150                 155                 160 


Val Gln Ile Pro Tyr Leu Thr Thr Glu Lys Asn Ser Phe Lys Arg Met 
                165                 170                 175     


Asp Asp Glu Asp Lys Gln Glu Thr Gln Ser Pro Thr Met Ser Pro Leu 
            180                 185                 190         


Ala Ser Pro Pro Ser Ser Pro Pro His Tyr Gln Arg Val Pro Leu Ser 
        195                 200                 205             


His Gly Tyr Ser Lys Leu Arg Ser Ser Ala Glu Gln Met His Pro Ala 
    210                 215                 220                 


Pro Tyr Glu Ala Arg Gln Pro Leu Val Gln Pro Glu Gly Ser Ser Ser 
225                 230                 235                 240 


Gly Gly Pro Gly Thr Lys Pro Leu Arg His Gln Ala Ser Leu Ile Arg 
                245                 250                 255     


Ser Phe Ser Val Glu Arg Glu Leu Gln Asp Asn Ser Ser Tyr Pro Asp 
            260                 265                 270         


Glu Pro Trp Arg Ile Thr Glu Glu Thr Leu Pro Gln Arg Glu Tyr Tyr 
        275                 280                 285             


Val Asn Gln Phe Arg Ser Leu Gln Pro Asp Pro Ser Ser Phe Ile Ser 
    290                 295                 300                 


Gly Ser Val Ala Lys Asn Phe Phe Thr Lys Ser Lys Leu Ser Ile Pro 
305                 310                 315                 320 


Glu Leu Ser Tyr Ile Trp Glu Leu Ser Asp Ala Asp Cys Asp Gly Ala 
                325                 330                 335     


Leu Glu Phe Cys Ala Ala Phe His Leu Ile Val Ala Arg Lys Asn Gly 
            340                 345                 350         


Tyr Pro Leu Pro Glu Gly Leu Pro Pro Thr Leu Gln Pro Glu Tyr Leu 
        355                 360                 365             


Gln Ala Ala Phe Pro Lys Pro Lys Trp Asp Cys Gln Leu Phe Asp Ser 
    370                 375                 380                 


Tyr Ser Glu Ser Leu Pro Ala Asn Gln Gln Pro Arg Asp Leu Asn Arg 
385                 390                 395                 400 


Met Glu Lys Thr Ser Val Lys Asp Met Ala Asp Leu Pro Val Pro Asn 
                405                 410                 415     


Gln Asp Val Thr Ser Asp Asp Lys Gln Ala Leu Lys Ser Thr Ile Asn 
            420                 425                 430         


Glu Ala Leu Pro Lys Asp Val Ser Glu Asp Pro Ala Thr Pro Lys Asp 
        435                 440                 445             


Ser Asn Ser Leu Lys Ala Arg Pro Arg Ser Arg Ser Tyr Ser Ser Thr 
    450                 455                 460                 


Ser Ile Glu Glu Ala Met Lys Arg Gly Glu Asp Pro Pro Thr Pro Pro 
465                 470                 475                 480 


Pro Arg Pro Gln Lys Thr His Ser Arg Ala Ser Ser Leu Asp Leu Asn 
                485                 490                 495     


Lys Val Phe Gln Pro Ser Val Pro Ala Thr Lys Ser Gly Leu Leu Pro 
            500                 505                 510         


<210>  28
<211>  1536
<212>  DNA
<213>  artificial sequence

<220>
<223>  POB1 cDNA for deletion mutant 1-512

<400>  28
atggaggcgg cagcggcggc ggcggcggcg gcagcggcag cggcagcggc gggcgggggc     60

tgtggctccg ggccgccgcc gctgctgctg agcgagggcg agcagcagtg ctactccgag    120

ctcttcgcgc gctgtgccgg cgccgcgggc gggggccccg ggtctgggcc ccccgaggcc    180

gccagagtcg cccccggcac ggccactgcg gccgccggcc ccgtggctga cctgtttcgg    240

gcatcgcagc tgcccgccga gacgctgcac cagatcacag aactgtgtgg tgcaaagcgg    300

gttggttatt ttggtccaac acagttttac attgccctga aattaattgc tgcagcacaa    360

tctggcctcc cggtacggat agagagtatt aaatgtgaat tgcctctgcc tcgctttatg    420

atgtcaaaga atgatggtga gatacgattt gggaacccag ctgagctgca tggaactaag    480

gttcagattc catatttaac tacagaaaaa aattccttca aaagaatgga cgatgaggat    540

aaacaggaaa cacagtctcc cacgatgtca cccctcgcct cccctccttc ttccccgcct    600

cattaccaga gggtgccctt gagccatggc tacagcaaac tgcggagcag cgcagaacag    660

atgcatccag caccttatga agctaggcag ccccttgtcc agcccgaggg atcctcatca    720

gggggcccag gaaccaagcc ccttcggcat caggcttccc ttatccggtc cttttcagtg    780

gagagggaac tacaggataa cagcagttac cccgacgaac cctggaggat aacagaagaa    840

cagcgcgagt actatgtcaa tcagttccga tcccttcagc cagacccaag ctctttcatt    900

tcaggttctg tggccaagaa cttcttcacc aaatcaaagc tttccattcc agaactctcc    960

tatatatggg agcttagtga tgctgactgt gatggagccc tgaccctgcc tgagttctgt   1020

gctgcgtttc atctcattgt ggctcggaag aacggctacc cattgcctga gggcctccct   1080

ccaactctgc agccagaata cctgcaggca gcttttccta agcccaaatg ggactgtcaa   1140

ttatttgatt cttattctga gtcactgccg gcaaatcaac aacctcgtga cttgaatcgg   1200

atggagaaga catctgttaa agacatggct gaccttcctg tccctaacca ggatgtaact   1260

agtgatgaca aacaagcttt gaaaagtact atcaatgaag ccttaccaaa ggacgtgtct   1320

gaggatccag caactcccaa ggattccaac agtctcaaag caagaccaag atccagatct   1380

tactctagca cctccataga agaggccatg aaaaggggcg aggaccctcc caccccgcca   1440

cctcggccac agaaaaccca ttccagagcc tcctccttgg atctgaataa agtcttccag   1500

cccagtgtgc cagctaccaa gtcaggattg ttaccc                             1536


<210>  29
<211>  26
<212>  DNA
<213>  artificial sequence

<220>
<223>  upstream primer for POB1 deletion mutant 1-512

<400>  29
ggcggatcca tggaggcggc agcggc                                          26


<210>  30
<211>  30
<212>  DNA
<213>  artificial sequence

<220>
<223>  downstream primer for POB1 deletion mutant 1-512

<400>  30
ccgctcgagg gtaacaatcc tgacttggta                                      30


