                         SEQUENCE LISTING

<110>  BASF Plant Science GmbH
 
<120>  Selection system for wheat

<130>  PF 57113

<160>  42    

<170>  PatentIn version 3.3

<210>  1
<211>  1329
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(1329)
<223>  E.coli D-serine dehydratase [dsdA] gene/CDS

<400>  1
atg gaa aac gct aaa atg aac tcg ctc atc gcc cag tat ccg ttg gta       48
Met Glu Asn Ala Lys Met Asn Ser Leu Ile Ala Gln Tyr Pro Leu Val         
1               5                   10                  15              

aag gat ctg gtt gct ctt aaa gaa acc acc tgg ttt aat cct ggc acg       96
Lys Asp Leu Val Ala Leu Lys Glu Thr Thr Trp Phe Asn Pro Gly Thr         
            20                  25                  30                  

acc tca ttg gct gaa ggt tta cct tat gtt ggc ctg acc gaa cag gat      144
Thr Ser Leu Ala Glu Gly Leu Pro Tyr Val Gly Leu Thr Glu Gln Asp         
        35                  40                  45                      

gtt cag gac gcc cat gcg cgc tta tcc cgt ttt gca ccc tat ctg gca      192
Val Gln Asp Ala His Ala Arg Leu Ser Arg Phe Ala Pro Tyr Leu Ala         
    50                  55                  60                          

aaa gca ttt cct gaa act gct gcc act ggg ggg att att gaa tca gaa      240
Lys Ala Phe Pro Glu Thr Ala Ala Thr Gly Gly Ile Ile Glu Ser Glu         
65                  70                  75                  80          

ctg gtt gcc att cca gct atg caa aaa cgg ctg gaa aaa gaa tat cag      288
Leu Val Ala Ile Pro Ala Met Gln Lys Arg Leu Glu Lys Glu Tyr Gln         
                85                  90                  95              

caa ccg atc agc ggg caa ctg tta ctg aaa aaa gat agc cat ttg ccc      336
Gln Pro Ile Ser Gly Gln Leu Leu Leu Lys Lys Asp Ser His Leu Pro         
            100                 105                 110                 

att tcc ggc tcc ata aaa gca cgc ggc ggg att tat gaa gtc ctg gca      384
Ile Ser Gly Ser Ile Lys Ala Arg Gly Gly Ile Tyr Glu Val Leu Ala         
        115                 120                 125                     

cac gca gaa aaa ctg gct ctg gaa gcg ggg ttg ctg acg ctt gat gat      432
His Ala Glu Lys Leu Ala Leu Glu Ala Gly Leu Leu Thr Leu Asp Asp         
    130                 135                 140                         

gac tac agc aaa ctg ctt tct ccg gag ttt aaa cag ttc ttt agc caa      480
Asp Tyr Ser Lys Leu Leu Ser Pro Glu Phe Lys Gln Phe Phe Ser Gln         
145                 150                 155                 160         

tac agc att gct gtg ggc tca acc gga aat ctg ggg tta tca atc ggc      528
Tyr Ser Ile Ala Val Gly Ser Thr Gly Asn Leu Gly Leu Ser Ile Gly         
                165                 170                 175             

att atg agc gcc cgc att ggc ttt aag gtg aca gtt cat atg tct gct      576
Ile Met Ser Ala Arg Ile Gly Phe Lys Val Thr Val His Met Ser Ala         
            180                 185                 190                 

gat gcc cgg gca tgg aaa aaa gcg aaa ctg cgc agc cat ggc gtt acg      624
Asp Ala Arg Ala Trp Lys Lys Ala Lys Leu Arg Ser His Gly Val Thr         
        195                 200                 205                     

gtc gtg gaa tat gag caa gat tat ggt gtt gcc gtc gag gaa gga cgt      672
Val Val Glu Tyr Glu Gln Asp Tyr Gly Val Ala Val Glu Glu Gly Arg         
    210                 215                 220                         

aaa gca gcg cag tct gac ccg aac tgt ttc ttt att gat gac gaa aat      720
Lys Ala Ala Gln Ser Asp Pro Asn Cys Phe Phe Ile Asp Asp Glu Asn         
225                 230                 235                 240         

tcc cgc acg ttg ttc ctt ggg tat tcc gtc gct ggc cag cgt ctt aaa      768
Ser Arg Thr Leu Phe Leu Gly Tyr Ser Val Ala Gly Gln Arg Leu Lys         
                245                 250                 255             

gcg caa ttt gcc cag caa ggc cgt atc gtc gat gct gat aac cct ctg      816
Ala Gln Phe Ala Gln Gln Gly Arg Ile Val Asp Ala Asp Asn Pro Leu         
            260                 265                 270                 

ttt gtc tat ctg ccg tgt ggt gtt ggc ggt ggt cct ggt ggc gtc gca      864
Phe Val Tyr Leu Pro Cys Gly Val Gly Gly Gly Pro Gly Gly Val Ala         
        275                 280                 285                     

ttc ggg ctt aaa ctg gcg ttt ggc gat cat gtt cac tgc ttt ttt gcc      912
Phe Gly Leu Lys Leu Ala Phe Gly Asp His Val His Cys Phe Phe Ala         
    290                 295                 300                         

gaa cca acg cac tcc cct tgt atg ttg tta ggc gtc cat aca gga tta      960
Glu Pro Thr His Ser Pro Cys Met Leu Leu Gly Val His Thr Gly Leu         
305                 310                 315                 320         

cac gat cag att tct gtt cag gat att ggt atc gac aac ctt acc gca     1008
His Asp Gln Ile Ser Val Gln Asp Ile Gly Ile Asp Asn Leu Thr Ala         
                325                 330                 335             

gcg gat ggc ctt gca gtt ggt cgc gca tca ggc ttt gtc ggg cgg gca     1056
Ala Asp Gly Leu Ala Val Gly Arg Ala Ser Gly Phe Val Gly Arg Ala         
            340                 345                 350                 

atg gag cgt ctg ctg gat ggc ttc tat acc ctt agc gat caa acc atg     1104
Met Glu Arg Leu Leu Asp Gly Phe Tyr Thr Leu Ser Asp Gln Thr Met         
        355                 360                 365                     

tat gac atg ctt ggc tgg ctg gcg cag gaa gaa ggt att cgt ctt gaa     1152
Tyr Asp Met Leu Gly Trp Leu Ala Gln Glu Glu Gly Ile Arg Leu Glu         
    370                 375                 380                         

cct tcg gca ctg gcg ggt atg gcc gga cct cag cgc gtg tgt gca tca     1200
Pro Ser Ala Leu Ala Gly Met Ala Gly Pro Gln Arg Val Cys Ala Ser         
385                 390                 395                 400         

gta agt tac caa cag atg cac ggt ttc agc gca gaa caa ctg cgt aat     1248
Val Ser Tyr Gln Gln Met His Gly Phe Ser Ala Glu Gln Leu Arg Asn         
                405                 410                 415             

acc act cat ctg gtg tgg gcg acg gga ggt gga atg gtg ccg gaa gaa     1296
Thr Thr His Leu Val Trp Ala Thr Gly Gly Gly Met Val Pro Glu Glu         
            420                 425                 430                 

gag atg aat caa tat ctg gca aaa ggc cgt taa                         1329
Glu Met Asn Gln Tyr Leu Ala Lys Gly Arg                                 
        435                 440                                         


<210>  2
<211>  442
<212>  PRT
<213>  Escherichia coli

<400>  2

Met Glu Asn Ala Lys Met Asn Ser Leu Ile Ala Gln Tyr Pro Leu Val 
1               5                   10                  15      


Lys Asp Leu Val Ala Leu Lys Glu Thr Thr Trp Phe Asn Pro Gly Thr 
            20                  25                  30          


Thr Ser Leu Ala Glu Gly Leu Pro Tyr Val Gly Leu Thr Glu Gln Asp 
        35                  40                  45              


Val Gln Asp Ala His Ala Arg Leu Ser Arg Phe Ala Pro Tyr Leu Ala 
    50                  55                  60                  


Lys Ala Phe Pro Glu Thr Ala Ala Thr Gly Gly Ile Ile Glu Ser Glu 
65                  70                  75                  80  


Leu Val Ala Ile Pro Ala Met Gln Lys Arg Leu Glu Lys Glu Tyr Gln 
                85                  90                  95      


Gln Pro Ile Ser Gly Gln Leu Leu Leu Lys Lys Asp Ser His Leu Pro 
            100                 105                 110         


Ile Ser Gly Ser Ile Lys Ala Arg Gly Gly Ile Tyr Glu Val Leu Ala 
        115                 120                 125             


His Ala Glu Lys Leu Ala Leu Glu Ala Gly Leu Leu Thr Leu Asp Asp 
    130                 135                 140                 


Asp Tyr Ser Lys Leu Leu Ser Pro Glu Phe Lys Gln Phe Phe Ser Gln 
145                 150                 155                 160 


Tyr Ser Ile Ala Val Gly Ser Thr Gly Asn Leu Gly Leu Ser Ile Gly 
                165                 170                 175     


Ile Met Ser Ala Arg Ile Gly Phe Lys Val Thr Val His Met Ser Ala 
            180                 185                 190         


Asp Ala Arg Ala Trp Lys Lys Ala Lys Leu Arg Ser His Gly Val Thr 
        195                 200                 205             


Val Val Glu Tyr Glu Gln Asp Tyr Gly Val Ala Val Glu Glu Gly Arg 
    210                 215                 220                 


Lys Ala Ala Gln Ser Asp Pro Asn Cys Phe Phe Ile Asp Asp Glu Asn 
225                 230                 235                 240 


Ser Arg Thr Leu Phe Leu Gly Tyr Ser Val Ala Gly Gln Arg Leu Lys 
                245                 250                 255     


Ala Gln Phe Ala Gln Gln Gly Arg Ile Val Asp Ala Asp Asn Pro Leu 
            260                 265                 270         


Phe Val Tyr Leu Pro Cys Gly Val Gly Gly Gly Pro Gly Gly Val Ala 
        275                 280                 285             


Phe Gly Leu Lys Leu Ala Phe Gly Asp His Val His Cys Phe Phe Ala 
    290                 295                 300                 


Glu Pro Thr His Ser Pro Cys Met Leu Leu Gly Val His Thr Gly Leu 
305                 310                 315                 320 


His Asp Gln Ile Ser Val Gln Asp Ile Gly Ile Asp Asn Leu Thr Ala 
                325                 330                 335     


Ala Asp Gly Leu Ala Val Gly Arg Ala Ser Gly Phe Val Gly Arg Ala 
            340                 345                 350         


Met Glu Arg Leu Leu Asp Gly Phe Tyr Thr Leu Ser Asp Gln Thr Met 
        355                 360                 365             


Tyr Asp Met Leu Gly Trp Leu Ala Gln Glu Glu Gly Ile Arg Leu Glu 
    370                 375                 380                 


Pro Ser Ala Leu Ala Gly Met Ala Gly Pro Gln Arg Val Cys Ala Ser 
385                 390                 395                 400 


Val Ser Tyr Gln Gln Met His Gly Phe Ser Ala Glu Gln Leu Arg Asn 
                405                 410                 415     


Thr Thr His Leu Val Trp Ala Thr Gly Gly Gly Met Val Pro Glu Glu 
            420                 425                 430         


Glu Met Asn Gln Tyr Leu Ala Lys Gly Arg 
        435                 440         


<210>  3
<211>  1107
<212>  DNA
<213>  Rhodosporidium toruloides


<220>
<221>  CDS
<222>  (1)..(1107)
<223>  Rhodosporidium toruloides D-amino acid oxidase CDS

<400>  3
atg cac tcg cag aag cgc gtc gtt gtc ctc gga tca ggc gtt atc ggt       48
Met His Ser Gln Lys Arg Val Val Val Leu Gly Ser Gly Val Ile Gly         
1               5                   10                  15              

ctg agc agc gcc ctc atc ctc gct cgg aag ggc tac agc gtg cat att       96
Leu Ser Ser Ala Leu Ile Leu Ala Arg Lys Gly Tyr Ser Val His Ile         
            20                  25                  30                  

ctc gcg cgc gac ttg ccg gag gac gtc tcg agc cag act ttc gct tca      144
Leu Ala Arg Asp Leu Pro Glu Asp Val Ser Ser Gln Thr Phe Ala Ser         
        35                  40                  45                      

cca tgg gct ggc gcg aat tgg acg cct ttc atg acg ctt aca gac ggt      192
Pro Trp Ala Gly Ala Asn Trp Thr Pro Phe Met Thr Leu Thr Asp Gly         
    50                  55                  60                          

cct cga caa gca aaa tgg gaa gaa tcg act ttc aag aag tgg gtc gag      240
Pro Arg Gln Ala Lys Trp Glu Glu Ser Thr Phe Lys Lys Trp Val Glu         
65                  70                  75                  80          

ttg gtc ccg acg ggc cat gcc atg tgg ctc aag ggg acg agg cgg ttc      288
Leu Val Pro Thr Gly His Ala Met Trp Leu Lys Gly Thr Arg Arg Phe         
                85                  90                  95              

gcg cag aac gaa gac ggc ttg ctc ggg cac tgg tac aag gac atc acg      336
Ala Gln Asn Glu Asp Gly Leu Leu Gly His Trp Tyr Lys Asp Ile Thr         
            100                 105                 110                 

cca aat tac cgc ccc ctc cca tct tcc gaa tgt cca cct ggc gct atc      384
Pro Asn Tyr Arg Pro Leu Pro Ser Ser Glu Cys Pro Pro Gly Ala Ile         
        115                 120                 125                     

ggc gta acc tac gac acc ctc tcc gtc cac gca cca aag tac tgc cag      432
Gly Val Thr Tyr Asp Thr Leu Ser Val His Ala Pro Lys Tyr Cys Gln         
    130                 135                 140                         

tac ctt gca aga gag ctg cag aag ctc ggc gcg acg ttt gag aga cgg      480
Tyr Leu Ala Arg Glu Leu Gln Lys Leu Gly Ala Thr Phe Glu Arg Arg         
145                 150                 155                 160         

acc gtt acg tcg ctt gag cag gcg ttc gac ggt gcg gat ttg gtg gtc      528
Thr Val Thr Ser Leu Glu Gln Ala Phe Asp Gly Ala Asp Leu Val Val         
                165                 170                 175             

aac gct acg gga ctt ggc gcc aag tcg att gcg ggc atc gac gac caa      576
Asn Ala Thr Gly Leu Gly Ala Lys Ser Ile Ala Gly Ile Asp Asp Gln         
            180                 185                 190                 

gcc gcc gag cca atc cgc ggg caa acc gtc ctc gtc aag tcc cca tgc      624
Ala Ala Glu Pro Ile Arg Gly Gln Thr Val Leu Val Lys Ser Pro Cys         
        195                 200                 205                     

aag cga tgc acg atg gac tcg tcc gac ccc gct tct ccc gcc tac atc      672
Lys Arg Cys Thr Met Asp Ser Ser Asp Pro Ala Ser Pro Ala Tyr Ile         
    210                 215                 220                         

att ccc cga cca ggt ggc gaa gtc atc tgc ggc ggg acg tac ggc gtg      720
Ile Pro Arg Pro Gly Gly Glu Val Ile Cys Gly Gly Thr Tyr Gly Val         
225                 230                 235                 240         

gga gac tgg gac ttg tct gtc aac cca gag acg gtc cag cgg atc ctc      768
Gly Asp Trp Asp Leu Ser Val Asn Pro Glu Thr Val Gln Arg Ile Leu         
                245                 250                 255             

aag cac tgc ttg cgc ctc gac ccg acc atc tcg agc gac gga acg atc      816
Lys His Cys Leu Arg Leu Asp Pro Thr Ile Ser Ser Asp Gly Thr Ile         
            260                 265                 270                 

gaa ggc atc gag gtc ctc cgc cac aac gtc ggc ttg cga cct gca cga      864
Glu Gly Ile Glu Val Leu Arg His Asn Val Gly Leu Arg Pro Ala Arg         
        275                 280                 285                     

cga ggc gga ccc cgc gtt gag gca gaa cgg atc gtc ctg cct ctc gac      912
Arg Gly Gly Pro Arg Val Glu Ala Glu Arg Ile Val Leu Pro Leu Asp         
    290                 295                 300                         

cgg aca aag tcg ccc ctc tcg ctc ggc agg ggc agc gca cga gcg gcg      960
Arg Thr Lys Ser Pro Leu Ser Leu Gly Arg Gly Ser Ala Arg Ala Ala         
305                 310                 315                 320         

aag gag aag gag gtc acg ctt gtg cat gcg tat ggc ttc tcg agt gcg     1008
Lys Glu Lys Glu Val Thr Leu Val His Ala Tyr Gly Phe Ser Ser Ala         
                325                 330                 335             

gga tac cag cag agt tgg ggc gcg gcg gag gat gtc gcg cag ctc gtc     1056
Gly Tyr Gln Gln Ser Trp Gly Ala Ala Glu Asp Val Ala Gln Leu Val         
            340                 345                 350                 

gac gag gcg ttc cag cgg tac cac ggc gcg gcg cgg gag tcg aag ttg     1104
Asp Glu Ala Phe Gln Arg Tyr His Gly Ala Ala Arg Glu Ser Lys Leu         
        355                 360                 365                     

tag                                                                 1107


<210>  4
<211>  368
<212>  PRT
<213>  Rhodosporidium toruloides

<400>  4

Met His Ser Gln Lys Arg Val Val Val Leu Gly Ser Gly Val Ile Gly 
1               5                   10                  15      


Leu Ser Ser Ala Leu Ile Leu Ala Arg Lys Gly Tyr Ser Val His Ile 
            20                  25                  30          


Leu Ala Arg Asp Leu Pro Glu Asp Val Ser Ser Gln Thr Phe Ala Ser 
        35                  40                  45              


Pro Trp Ala Gly Ala Asn Trp Thr Pro Phe Met Thr Leu Thr Asp Gly 
    50                  55                  60                  


Pro Arg Gln Ala Lys Trp Glu Glu Ser Thr Phe Lys Lys Trp Val Glu 
65                  70                  75                  80  


Leu Val Pro Thr Gly His Ala Met Trp Leu Lys Gly Thr Arg Arg Phe 
                85                  90                  95      


Ala Gln Asn Glu Asp Gly Leu Leu Gly His Trp Tyr Lys Asp Ile Thr 
            100                 105                 110         


Pro Asn Tyr Arg Pro Leu Pro Ser Ser Glu Cys Pro Pro Gly Ala Ile 
        115                 120                 125             


Gly Val Thr Tyr Asp Thr Leu Ser Val His Ala Pro Lys Tyr Cys Gln 
    130                 135                 140                 


Tyr Leu Ala Arg Glu Leu Gln Lys Leu Gly Ala Thr Phe Glu Arg Arg 
145                 150                 155                 160 


Thr Val Thr Ser Leu Glu Gln Ala Phe Asp Gly Ala Asp Leu Val Val 
                165                 170                 175     


Asn Ala Thr Gly Leu Gly Ala Lys Ser Ile Ala Gly Ile Asp Asp Gln 
            180                 185                 190         


Ala Ala Glu Pro Ile Arg Gly Gln Thr Val Leu Val Lys Ser Pro Cys 
        195                 200                 205             


Lys Arg Cys Thr Met Asp Ser Ser Asp Pro Ala Ser Pro Ala Tyr Ile 
    210                 215                 220                 


Ile Pro Arg Pro Gly Gly Glu Val Ile Cys Gly Gly Thr Tyr Gly Val 
225                 230                 235                 240 


Gly Asp Trp Asp Leu Ser Val Asn Pro Glu Thr Val Gln Arg Ile Leu 
                245                 250                 255     


Lys His Cys Leu Arg Leu Asp Pro Thr Ile Ser Ser Asp Gly Thr Ile 
            260                 265                 270         


Glu Gly Ile Glu Val Leu Arg His Asn Val Gly Leu Arg Pro Ala Arg 
        275                 280                 285             


Arg Gly Gly Pro Arg Val Glu Ala Glu Arg Ile Val Leu Pro Leu Asp 
    290                 295                 300                 


Arg Thr Lys Ser Pro Leu Ser Leu Gly Arg Gly Ser Ala Arg Ala Ala 
305                 310                 315                 320 


Lys Glu Lys Glu Val Thr Leu Val His Ala Tyr Gly Phe Ser Ser Ala 
                325                 330                 335     


Gly Tyr Gln Gln Ser Trp Gly Ala Ala Glu Asp Val Ala Gln Leu Val 
            340                 345                 350         


Asp Glu Ala Phe Gln Arg Tyr His Gly Ala Ala Arg Glu Ser Lys Leu 
        355                 360                 365             


<210>  5
<211>  275
<212>  DNA
<213>  Zea mays


<220>
<221>  promoter
<222>  (1)..(275)
<223>  Zea mays ubiquitin 275 bp core promoter

<220>
<221>  misc_signal
<222>  (61)..(74)
<223>  Similar to consensus heat shock element

<220>
<221>  misc_signal
<222>  (71)..(84)
<223>  Similar to consensus heat shock element

<220>
<221>  TATA_signal
<222>  (246)..(254)
<223>  TATA Box

<400>  5
aaccagcagc gtcgcgtcgg gccaagcgaa gcagacggca cggcatctct gtcgctgcct     60

ctggacccct ctcgagagtt ccgctccacc gttggacttg ctccgctgtc ggcatccaga    120

aattgcgtgg cggagcggca gacgtgagcc ggcacggcag gcggcctcct cctcctctca    180

cggcaccggc agctacgggg gattcctttc ccaccgctcc ttcgctttcc cttcctcgcc    240

cgccgtaata aatagacacc ccctccacac cctct                               275


<210>  6
<211>  1988
<212>  DNA
<213>  Zea mays


<220>
<221>  promoter
<222>  (1)..(1988)
<223>  Zea mays ubiquitin promoter region with 5'UTR and intron

<220>
<221>  promoter
<222>  (1)..(899)
<223>  Zea mays ubiquitin promoter region

<220>
<221>  misc_signal
<222>  (685)..(698)
<223>  Similar to consensus heat shock element

<220>
<221>  misc_signal
<222>  (695)..(708)
<223>  Similar to consensus heat shock element

<220>
<221>  TATA_signal
<222>  (870)..(878)
<223>  TATA Box

<220>
<221>  5'UTR
<222>  (900)..(1988)

<220>
<221>  Intron
<222>  (983)..(1988)
<223>  Zea mays ubiquitin gene intron MubG1

<400>  6
tgcagtgcag cgtgacccgg tcgtgcccct ctctagagat aatgagcatt gcatgtctaa     60

gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca gtttatctat    120

ctttatacat atatttaaac tttactctac gaataatata atctatagta ctacaataat    180

atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag gacaattgag    240

tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt ctcctttttt    300

tttgcaaata gcttcaccta tataatactt catccatttt attagtacat ccatttaggg    360

tttagggtta atggttttta tagactaatt tttttagtac atctatttta ttctatttta    420

gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata gtttagatat    480

aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga aattaaaaaa    540

actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa cgccgtcgac    600

gagtctaacg gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag cgaagcagac    660

ggcacggcat ctctgtcgct gcctctggac ccctctcgag agttccgctc caccgttgga    720

cttgctccgc tgtcggcatc cagaaattgc gtggcggagc ggcagacgtg agccggcacg    780

gcaggcggcc tcctcctcct ctcacggcac cggcagctac gggggattcc tttcccaccg    840

ctccttcgct ttcccttcct cgcccgccgt aataaataga caccccctcc acaccctctt    900

tccccaacct cgtgttgttc ggagcgcaca cacacacaac cagatctccc ccaaatccac    960

ccgtcggcac ctccgcttca aggtacgccg ctcgtcctcc cccccccccc ccctctctac   1020

cttctctaga tcggcgttcc ggtccatggt tagggcccgg tagttctact tctgttcatg   1080

tttgtgttag atccgtgttt gtgttagatc cgtgctgcta gcgttcgtac acggatgcga   1140

cctgtacgtc agacacgttc tgattgctaa cttgccagtg tttctctttg gggaatcctg   1200

ggatggctct agccgttccg cagacgggat cgatttcatg attttttttg tttcgttgca   1260

tagggtttgg tttgcccttt tcctttattt caatatatgc cgtgcacttg tttgtcgggt   1320

catcttttca tgcttttttt tgtcttggtt gtgatgatgt ggtctggttg ggcggtcgtt   1380

ctagatcgga gtagaattct gtttcaaact acctggtgga tttattaatt ttggatctgt   1440

atgtgtgtgc catacatatt catagttacg aattgaagat gatggatgga aatatcgatc   1500

taggataggt atacatgttg atgcgggttt tactgatgca tatacagaga tgctttttgt   1560

tcgcttggtt gtgatgatgt ggtgtggttg ggcggtcgtt cattcgttct agatcggagt   1620

agaatactgt ttcaaactac ctggtgtatt tattaatttt ggaactgtat gtgtgtgtca   1680

tacatcttca tagttacgag tttaagatgg atggaaatat cgatctagga taggtataca   1740

tgttgatgtg ggttttactg atgcatatac atgatggcat atgcagcatc tattcatatg   1800

ctctaacctt gagtacctat ctattataat aaacaagtat gttttataat tatttcgatc   1860

ttgatatact tggatgatgg catatgcagc agctatatgt ggattttttt agccctgcct   1920

tcatacgcta tttatttgct tggtactgtt tcttttgtcg atgctcaccc tgttgtttgg   1980

tgttactt                                                            1988


<210>  7
<211>  275
<212>  DNA
<213>  Sugarcane bacilliform virus


<220>
<221>  promoter
<222>  (1)..(275)
<223>  Sugarcane bacilliform virus 275 bp core promoter

<220>
<221>  TATA_signal
<222>  (224)..(243)
<223>  TATA Box

<400>  7
gattgaggag gcattgacgt cagggatgac cgcagcggag agtactgggc ccattcagtg     60

gatgctccac tgagttgtat tattgtgtgc ttttcggaca agtgtgctgt ccactttctt    120

ttggcacctg tgccacttta ttccttgtct gccacgatgc ctttgcttag cttgtaagca    180

aggatcgcag tgcgtgtgtg acaccacccc ccttccgacg ctctgcctat ataaggcacc    240

gtctgtaagc tcttacgatc atcggtagtt cacca                               275


<210>  8
<211>  1398
<212>  DNA
<213>  Sugarcane bacilliform virus


<220>
<221>  promoter
<222>  (1)..(1398)
<223>  Sugarcane bacilliform virus [ScBV] promoter region

<220>
<221>  misc_signal
<222>  (98)..(118)
<223>  Heat shock factor

<220>
<221>  misc_signal
<222>  (365)..(385)
<223>  Heat shock factor

<220>
<221>  misc_signal
<222>  (606)..(626)
<223>  Heat shock factor

<220>
<221>  misc_signal
<222>  (705)..(719)
<223>  Heat shock element

<220>
<221>  TATA_signal
<222>  (1345)..(1364)
<223>  TATA Box

<400>  8
aatcctggct agcaacactg aactatgcca gaaaccacat caaagatatg ggcaagcttc     60

ttggcccatt atatccaaag acctcagaga aaggtgagcg aaggctcaat tcagaagatt    120

ggaagctgat caataggatc aagacaatgg tgagaacgct tccaaatctc actattccac    180

cagaagatgc atacattatc attgaaacag atgcatgtgc aactggatgg ggagcagtat    240

gcaagtggaa gaaaaacaag gcagacccaa gaaatacaga gcaaatctgt aggtatgcca    300

gtggaaaatt tgataagcca aaaggaacct gtgatgcaga aatctatggg gttatgaatg    360

gcttagaaaa gatgagattg ttctacttgg acaaaagaga gatcacagtc agaactgaca    420

gtagtgcaat cgaaaggttc tacaacaaga gtgctgaaca caagccttct gagatcagat    480

ggatcaggtt catggactac atcactggtg caggaccaga gatagtcatt gaacacataa    540

aagggaagag caatggttta gctgacatct tgtccaggct caaagccaaa ttagctcaga    600

atgaaccaac ggaagagatg atcctgctta cacaagccat aagggaagta attccttatc    660

cagatcatcc atacactgag caactcagag aatggggaaa caaaattctg gatccattcc    720

ccacattcaa gaaggacatg ttcgaaagaa cagagcaagc ttttatgcta acagaggaac    780

cagttctact ctgtgcatgc aggaagcctg caattcagtt agtgtccaga acatctgcca    840

acccaggaag gaaattcttc aagtgcgcaa tgaacaaatg ccattgctgg tactgggcag    900

atctcattga agaacacatt caagacagaa ttgatgaatt tctcaagaat cttgaagttc    960

tgaagaccgg tggcgtgcaa acaatggagg aggaacttat gaaggaagtc accaagctga   1020

agatagaaga gcaggagttc gaggaatacc aggccacacc aagggctatg tcgccagtag   1080

ccgcagaaga tgtgctagat ctccaagacg taagcaatga cgattgagga ggcattgacg   1140

tcagggatga ccgcagcgga gagtactggg cccattcagt ggatgctcca ctgagttgta   1200

ttattgtgtg cttttcggac aagtgtgctg tccactttct tttggcacct gtgccacttt   1260

attccttgtc tgccacgatg cctttgctta gcttgtaagc aaggatcgca gtgcgtgtgt   1320

gacaccaccc cccttccgac gctctgccta tataaggcac cgtctgtaag ctcttacgat   1380

catcggtagt tcaccaag                                                 1398


<210>  9
<211>  5035
<212>  DNA
<213>  Artificial

<220>
<223>  Nucleic acid sequence encoding pRLM175, a kanamycin resistant 
       pSB11-type binary vector.


<220>
<221>  misc_structure
<222>  (5)..(132)
<223>  Left T-DNA border from pSB11

<220>
<221>  misc_binding
<222>  (51)..(75)
<223>  Left T-DNA border repeat region from pSB1

<220>
<221>  misc_structure
<222>  (232)..(376)
<223>  Right T-DNA border from pSB11

<220>
<221>  misc_binding
<222>  (272)..(295)
<223>  Right T-DNA border repeat region from pSB1

<220>
<221>  misc_structure
<222>  (777)..(3511)
<223>  pSB1 recombination target

<220>
<221>  rep_origin
<222>  (2654)..(2934)
<223>  ColEI replication origin from pSB11

<220>
<221>  misc_feature
<222>  (3074)..(3334)
<223>  ColEI BOM site from pSB11

<220>
<221>  misc_feature
<222>  (3793)..(4608)
<223>  cds encoding Kanamycin Resistance selection gene from Tn903

<400>  9
agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg tggcaggata     60

tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg gacgttttta    120

atgtactgaa ttggatccgc ccgggcggta ccgaattcgc ggccgcaagc ttgtacaacg    180

cgtaccggtt aattaatcta gaggcgcgcc gggcccggcc ggccagatct tgattgtcgt    240

ttcccgcctt cagtttaaac tatcagtgtt tgacaggata tattggcggg taaacctaag    300

agaaaagagc gtttattaga ataatcggat atttaaaagg gcgtgaaaag gtttatccgt    360

tcgtccattt gtatgtgcat gccaaccaca gggttcccct cgggagtgct tggcattccg    420

tgcgataatg acttctgttc aaccacccaa acgtcggaaa gcctgacgac ggagcagcat    480

tccaaaaaga tcccttggct cgtctgggtc ggctagaagg tcgagtgggc tgctgtggct    540

tgatccctca acgcggtcgc ggacgtagcg cagcgccgaa aaatcctcga tcgcaaatcc    600

gacgctgtcg aaaagcgtga tctgcttgtc gctctttcgg ccgacgtcct ggccagtcat    660

cacgcgccaa agttccgtca caggatgatc tggcgcgagt tgctggatct cgccttcaat    720

ccgggtctgt ggcgggaact ccacgaaaat atccgaacgc agcaagatcg tcgaccaatt    780

cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa    840

tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt    900

tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc    960

ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc   1020

ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa   1080

aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg   1140

gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag   1200

ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc   1260

gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta   1320

cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg   1380

cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca   1440

acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac   1500

caaacgacga gcgtgacacc acgatgccgg gggggggggg gggggacatg aggttgcccc   1560

gtattcagtg tcgctgattt gtattgtctg aagttgtttt tacgttaagt tgatgcagat   1620

caattaatac gatacctgcg tcataattga ttatttgacg tggtttgatg gcctccacgc   1680

acgttgtgat atgtagatga taatcattat cactttacgg gtcctttccg gtgatccgac   1740

aggttacggg gcggcgacct cgcgggtttt cgctatttat gaaaattttc cggtttaagg   1800

cgtttccgtt cttcttcgtc ataacttaat gtttttattt aaaataccct ctgaaaagaa   1860

aggaaacgac aggtgctgaa agcgagcttt ttggcctctg tcgtttcctt tctctgtttt   1920

tgtccgtgga atgaacaatg gaaccccccc cccccccccc tgcagcaatg gcaacaacgt   1980

tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact   2040

ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt   2100

ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg   2160

ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta   2220

tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac   2280

tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta   2340

aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt   2400

tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt   2460

tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt   2520

gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc   2580

agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg   2640

tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg   2700

ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt   2760

cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac   2820

tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg   2880

acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg   2940

gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat   3000

ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt   3060

tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg   3120

attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa   3180

cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc   3240

tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct   3300

ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc   3360

tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc   3420

atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc   3480

gtcatcaccg aaacgcgcga ggcagcagat cccccgatca agtagataca ctacatatat   3540

ctacaataga catcgagccg gaaggtgatg tttactttcc tgaaatcccc agcaatttta   3600

ggccagtttt tacccaagac ttcgcctcta acataaatta tagttaccaa atctggcaaa   3660

agggttgacc gggggggggg ggaaagccac gttgtgtctc aaaatctctg atgttacatt   3720

gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat   3780

acaaggggtg ttatgagcca tattcaacgg gaaacgtctt gctcgaggcc gcgattaaat   3840

tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca   3900

ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat   3960

ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg   4020

gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta   4080

ctcaccactg cgatccccgg gaaaacagca ttccaggtat tagaagaata tcctgattca   4140

ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt   4200

tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg   4260

aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa   4320

caagtctgga aagaaatgca taagcttttg ccattctcac cggattcagt cgtcactcat   4380

ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat   4440

gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc   4500

ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct   4560

gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt   4620

aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgg ctttgttgaa   4680

taaatcgaac ttttgctgag ttgaaggatc agatcacgca tcttcccgac aacgcagacc   4740

gttccgtggc aaagcaaaag ttcaaaatca ccaactggtc cacctacaac aaagctctca   4800

tcaaccgtgg ctccctcact ttctggctgg atgatggggc gattcaggga tcacaggcag   4860

caacgctctg tcatcgttac aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa   4920

acccggcagc ttagttgccg ttcttccgaa tagcatcggt aacatgagca aagtctgccg   4980

ccttacaacg gctctcccgc tgacgccgtc ccggactgat gggctgcctg tatcg        5035


<210>  10
<211>  8269
<212>  DNA
<213>  Artificial

<220>
<223>  Nucleic acid sequence encoding T-DNA region of pRLM166, a pRLM175
       derived binary vector containing p-ZmUBI+I::c-dsdA::t-OCS and 
       p-ScBV::c-gusINT::t-NOS cassettes.


<220>
<221>  misc_feature
<222>  (1)..(128)
<223>  Left T-DNA border from pSB11

<220>
<221>  misc_feature
<222>  (47)..(71)
<223>  Left T-DNA border repeat from pSB11

<220>
<221>  promoter
<222>  (162)..(2149)
<223>  Zea mays ubiquitin promoter with first intron

<220>
<221>  Intron
<222>  (1144)..(2149)
<223>  Zea mays ubiquitin gene first intron

<220>
<221>  misc_feature
<222>  (2192)..(3520)
<223>  cds encoding E.coli D-serine dehydratase [dsdA]

<220>
<221>  terminator
<222>  (3558)..(4270)
<223>  an octapine synthase terminator

<220>
<221>  promoter
<222>  (4343)..(5740)
<223>  Sugarcane bacilliform virus [ScBV] promoter

<220>
<221>  misc_feature
<222>  (5772)..(7772)
<223>  gusINT cds and intron

<220>
<221>  Intron
<222>  (6157)..(6345)
<223>  PIV2 intron of gusINT, a modified potato ST-LS1 intron

<220>
<221>  terminator
<222>  (7843)..(8095)
<223>  3'UTR of Nopaline Synthase from Agrobacterium tumefaciens T-DNA 
       used as terminator

<220>
<221>  misc_feature
<222>  (8125)..(8269)
<223>  right T-DNA border from pSB11

<220>
<221>  misc_feature
<222>  (8165)..(8188)
<223>  right T-DNA border repeat from pSB11

<400>  10
gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt     60

gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt    120

actgaattgg atccgcccgg gcggtaccaa gcttccgcgg ctgcagtgca gcgtgacccg    180

gtcgtgcccc tctctagaga taatgagcat tgcatgtcta agttataaaa aattaccaca    240

tatttttttt gtcacacttg tttgaagtgc agtttatcta tctttataca tatatttaaa    300

ctttactcta cgaataatat aatctatagt actacaataa tatcagtgtt ttagagaatc    360

atataaatga acagttagac atggtctaaa ggacaattga gtattttgac aacaggactc    420

tacagtttta tctttttagt gtgcatgtgt tctccttttt ttttgcaaat agcttcacct    480

atataatact tcatccattt tattagtaca tccatttagg gtttagggtt aatggttttt    540

atagactaat ttttttagta catctatttt attctatttt agcctctaaa ttaagaaaac    600

taaaactcta ttttagtttt tttatttaat agtttagata taaaatagaa taaaataaag    660

tgactaaaaa ttaaacaaat accctttaag aaattaaaaa aactaaggaa acatttttct    720

tgtttcgagt agataatgcc agcctgttaa acgccgtcga cgagtctaac ggacaccaac    780

cagcgaacca gcagcgtcgc gtcgggccaa gcgaagcaga cggcacggca tctctgtcgc    840

tgcctctgga cccctctcga gagttccgct ccaccgttgg acttgctccg ctgtcggcat    900

ccagaaattg cgtggcggag cggcagacgt gagccggcac ggcaggcggc ctcctcctcc    960

tctcacggca ccggcagcta cgggggattc ctttcccacc gctccttcgc tttcccttcc   1020

tcgcccgccg taataaatag acaccccctc cacaccctct ttccccaacc tcgtgttgtt   1080

cggagcgcac acacacacaa ccagatctcc cccaaatcca cccgtcggca cctccgcttc   1140

aaggtacgcc gctcgtcctc cccccccccc cccctctcta ccttctctag atcggcgttc   1200

cggtccatgg ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt   1260

tgtgttagat ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt   1320

ctgattgcta acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc   1380

gcagacggga tcgatttcat gatttttttt gtttcgttgc atagggtttg gtttgccctt   1440

ttcctttatt tcaatatatg ccgtgcactt gtttgtcggg tcatcttttc atgctttttt   1500

ttgtcttggt tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtagaattc   1560

tgtttcaaac tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat   1620

tcatagttac gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt   1680

gatgcgggtt ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg   1740

tggtgtggtt gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta   1800

cctggtgtat ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga   1860

gtttaagatg gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact   1920

gatgcatata catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta   1980

tctattataa taaacaagta tgttttataa ttatttcgat cttgatatac ttggatgatg   2040

gcatatgcag cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc   2100

ttggtactgt ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcagggtac   2160

ggatcctcat ctaagcgcaa agagacgtac tatggaaaac gctaaaatga actcgctcat   2220

cgcccagtat ccgttggtaa aggatctggt tgctcttaaa gaaaccacct ggtttaatcc   2280

tggcacgacc tcattggctg aaggtttacc ttatgttggc ctgaccgaac aggatgttca   2340

ggacgcccat gcgcgcttat cccgttttgc accctatctg gcaaaagcat ttcctgaaac   2400

tgctgccact ggggggatta ttgaatcaga actggttgcc attccagcta tgcaaaaacg   2460

gctggaaaaa gaatatcagc aaccgatcag cgggcaactg ttactgaaaa aagatagcca   2520

tttgcccatt tccggctcca taaaagcacg cggcgggatt tatgaagtcc tggcacacgc   2580

agaaaaactg gctctggaag cggggttgct gacgcttgat gatgactaca gcaaactgct   2640

ttctccggag tttaaacagt tctttagcca atacagcatt gctgtgggct caaccggaaa   2700

tctggggtta tcaatcggca ttatgagcgc ccgcattggc tttaaggtga cagttcatat   2760

gtctgctgat gcccgggcat ggaaaaaagc gaaactgcgc agccatggcg ttacggtcgt   2820

ggaatatgag caagattatg gtgttgccgt cgaggaagga cgtaaagcag cgcagtctga   2880

cccgaactgt ttctttattg atgacgaaaa ttcccgcacg ttgttccttg ggtattccgt   2940

cgctggccag cgtcttaaag cgcaatttgc ccagcaaggc cgtatcgtcg atgctgataa   3000

ccctctgttt gtctatctgc cgtgtggtgt tggcggtggt cctggtggcg tcgcattcgg   3060

gcttaaactg gcgtttggcg atcatgttca ctgctttttt gccgaaccaa cgcactcccc   3120

ttgtatgttg ttaggcgtcc atacaggatt acacgatcag atttctgttc aggatattgg   3180

tatcgacaac cttaccgcag cggatggcct tgcagttggt cgcgcatcag gctttgtcgg   3240

gcgggcaatg gagcgtctgc tggatggctt ctataccctt agcgatcaaa ccatgtatga   3300

catgcttggc tggctggcgc aggaagaagg tattcgtctt gaaccttcgg cactggcggg   3360

tatggccgga cctcagcgcg tgtgtgcatc agtaagttac caacagatgc acggtttcag   3420

cgcagaacaa ctgcgtaata ccactcatct ggtgtgggcg acgggaggtg gaatggtgcc   3480

ggaagaagag atgaatcaat atctggcaaa aggccgttaa taacgtttca acgcagcatg   3540

gatcgtaccg agctcaatcg atcctgcttt aatgagatat gcgagacgcc tatgatcgca   3600

tgatatttgc tttcaattct gttgtgcacg ttgtaaaaaa cctgagcatg tgtagctcag   3660

atccttaccg ccggtttcgg ttcattctaa tgaatatatc acccgttact atcgtatttt   3720

tatgaataat attctccgtt caatttactg attgtaccct actacttata tgtacaatat   3780

taaaatgaaa acaatatatt gtgctgaata ggtttatagc gacatctatg atagagcgcc   3840

acaataacaa acaattgcgt tttattatta caaatccaat tttaaaaaaa gcggcagaac   3900

cggtcaaacc taaaagactg attacataaa tcttattcaa atttcaaaag tgccccaggg   3960

gctagtatct acgacacacc gagcggcgaa ctaataacgc tcactgaagg gaactccggt   4020

tccccgccgg cgcgcatggg tgagattcct tgaagttgag tattggccgt ccgctctacc   4080

gaaagttacg ggcaccattc aacccggtcc agcacggcgg ccgggtaacc gacttgctgc   4140

cccgagaatt atgcagcatt tttttggtgt atgtgggccc caaatgaagt gcaggtcaaa   4200

ccttgacagt gacgacaaat cgttgggcgg gtccagggcg aattttgcga caacatgtcg   4260

aggctcagca ggatgggccc aggtacagaa ttcgcggccg tacaacgcgt accggttaat   4320

taaggtaccc aattgcatat gtaatcctgg ctagcaacac tgaactatgc cagaaaccac   4380

atcaaagata tgggcaagct tcttggccca ttatatccaa agacctcaga gaaaggtgag   4440

cgaaggctca attcagaaga ttggaagctg atcaatagga tcaagacaat ggtgagaacg   4500

cttccaaatc tcactattcc accagaagat gcatacatta tcattgaaac agatgcatgt   4560

gcaactggat ggggagcagt atgcaagtgg aagaaaaaca aggcagaccc aagaaataca   4620

gagcaaatct gtaggtatgc cagtggaaaa tttgataagc caaaaggaac ctgtgatgca   4680

gaaatctatg gggttatgaa tggcttagaa aagatgagat tgttctactt ggacaaaaga   4740

gagatcacag tcagaactga cagtagtgca atcgaaaggt tctacaacaa gagtgctgaa   4800

cacaagcctt ctgagatcag atggatcagg ttcatggact acatcactgg tgcaggacca   4860

gagatagtca ttgaacacat aaaagggaag agcaatggtt tagctgacat cttgtccagg   4920

ctcaaagcca aattagctca gaatgaacca acggaagaga tgatcctgct tacacaagcc   4980

ataagggaag taattcctta tccagatcat ccatacactg agcaactcag agaatgggga   5040

aacaaaattc tggatccatt ccccacattc aagaaggaca tgttcgaaag aacagagcaa   5100

gcttttatgc taacagagga accagttcta ctctgtgcat gcaggaagcc tgcaattcag   5160

ttagtgtcca gaacatctgc caacccagga aggaaattct tcaagtgcgc aatgaacaaa   5220

tgccattgct ggtactgggc agatctcatt gaagaacaca ttcaagacag aattgatgaa   5280

tttctcaaga atcttgaagt tctgaagacc ggtggcgtgc aaacaatgga ggaggaactt   5340

atgaaggaag tcaccaagct gaagatagaa gagcaggagt tcgaggaata ccaggccaca   5400

ccaagggcta tgtcgccagt agccgcagaa gatgtgctag atctccaaga cgtaagcaat   5460

gacgattgag gaggcattga cgtcagggat gaccgcagcg gagagtactg ggcccattca   5520

gtggatgctc cactgagttg tattattgtg tgcttttcgg acaagtgtgc tgtccacttt   5580

cttttggcac ctgtgccact ttattccttg tctgccacga tgcctttgct tagcttgtaa   5640

gcaaggatcg cagtgcgtgt gtgacaccac cccccttccg acgctctgcc tatataaggc   5700

accgtctgta agctcttacg atcatcggta gttcaccaag ggggtacgat ctccgggtag   5760

gtcagtccct tatgttacgt cctgtagaaa ccccaacccg tgaaatcaaa aaactcgacg   5820

gcctgtgggc attcagtctg gatcgcgaaa actgtggaat tggtcagcgt tggtgggaaa   5880

gcgcgttaca agaaagccgg gcaattgctg tgccaggcag ttttaacgat cagttcgccg   5940

atgcagatat tcgtaattat gcgggcaacg tctggtatca gcgcgaagtc tttataccga   6000

aaggttgggc aggccagcgt atcgtgctgc gtttcgatgc ggtcactcat tacggcaaag   6060

tgtgggtcaa taatcaggaa gtgatggagc atcagggcgg ctatacgcca tttgaagccg   6120

atgtcacgcc gtatgttatt gccgggaaaa gtgtacgtaa gtttctgctt ctacctttga   6180

tatatatata ataattatca ttaattagta gtaatataat atttcaaata tttttttcaa   6240

aataaaagaa tgtagtatat agcaattgct tttctgtagt ttataagtgt gtatatttta   6300

atttataact tttctaatat atgaccaaaa tttgttgatg tgcaggtatc accgtttgtg   6360

tgaacaacga actgaactgg cagactatcc cgccgggaat ggtgattacc gacgaaaacg   6420

gcaagaaaaa gcagtcttac ttccatgatt tctttaacta tgccggaatc catcgcagcg   6480

taatgctcta caccacgccg aacacctggg tggacgatat caccgtggtg acgcatgtcg   6540

cgcaagactg taaccacgcg tctgttgact ggcaggtggt ggccaatggt gatgtcagcg   6600

ttgaactgcg tgatgcggat caacaggtgg ttgcaactgg acaaggcact agcgggactt   6660

tgcaagtggt gaatccgcac ctctggcaac cgggtgaagg ttatctctat gaactgtgcg   6720

tcacagccaa aagccagaca gagtgtgata tctacccgct tcgcgtcggc atccggtcag   6780

tggcagtgaa gggcgaacag ttcctgatta accacaaacc gttctacttt actggctttg   6840

gtcgtcatga agatgcggac ttgcgtggca aaggattcga taacgtgctg atggtgcacg   6900

accacgcatt aatggactgg attggggcca actcctaccg tacctcgcat tacccttacg   6960

ctgaagagat gctcgactgg gcagatgaac atggcatcgt ggtgattgat gaaactgctg   7020

ctgtcggctt taacctctct ttaggcattg gtttcgaagc gggcaacaag ccgaaagaac   7080

tgtacagcga agaggcagtc aacggggaaa ctcagcaagc gcacttacag gcgattaaag   7140

agctgatagc gcgtgacaaa aaccacccaa gcgtggtgat gtggagtatt gccaacgaac   7200

cggatacccg tccgcaaggt gcacgggaat atttcgcgcc actggcggaa gcaacgcgta   7260

aactcgaccc gacgcgtccg atcacctgcg tcaatgtaat gttctgcgac gctcacaccg   7320

ataccatcag cgatctcttt gatgtgctgt gcctgaaccg ttattacgga tggtatgtcc   7380

aaagcggcga tttggaaacg gcagagaagg tactggaaaa agaacttctg gcctggcagg   7440

agaaactgca tcagccgatt atcatcaccg aatacggcgt ggatacgtta gccgggctgc   7500

actcaatgta caccgacatg tggagtgaag agtatcagtg tgcatggctg gatatgtatc   7560

accgcgtctt tgatcgcgtc agcgccgtcg tcggtgaaca ggtatggaat ttcgccgatt   7620

ttgcgacctc gcaaggcata ttgcgcgttg gcggtaacaa gaaagggatc ttcactcgcg   7680

accgcaaacc gaagtcggcg gcttttctgc tgcaaaaacg ctggactggc atgaacttcg   7740

gtgaaaaacc gcagcaggga ggcaaacaat gaatcaacaa ctctcctggc gcaccatcgt   7800

cggctacagc ctcgggaatt gctaccgagc tcgaatttcc ccgatcgttc aaacatttgg   7860

caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt   7920

ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga   7980

tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata   8040

tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa   8100

ttcgggcccg gccggccaga tcttgattgt cgtttcccgc cttcagttta aactatcagt   8160

gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg   8220

gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgt               8269


<210>  11
<211>  7838
<212>  DNA
<213>  Artificial

<220>
<223>  Nucleic acid sequence encoding T-DNA region of pRLM167, a pRLM175
       derived binary vector containing p-ZmUBI+I::c-dsdA::t-OCS and 
       p-ZmUBI+I::c-PAT::t-OCS cassettes.


<220>
<221>  misc_feature
<222>  (1)..(128)
<223>  Left T-DNA border from pSB11

<220>
<221>  misc_feature
<222>  (47)..(71)
<223>  Left T-DNA border repeat from pSB11

<220>
<221>  promoter
<222>  (162)..(2149)
<223>  Zea mays ubiquitin promoter with first intron

<220>
<221>  Intron
<222>  (1144)..(2149)
<223>  Zea mays ubiquitin gene first intron

<220>
<221>  misc_feature
<222>  (2192)..(3520)
<223>  cds encoding E.coli D-serine dehydratase [dsdA]

<220>
<221>  terminator
<222>  (3558)..(4270)
<223>  an octapine synthase terminator

<220>
<221>  promoter
<222>  (4318)..(6305)
<223>  Zea mays ubiquitin promoter with first intron

<220>
<221>  Intron
<222>  (5300)..(6305)
<223>  Zea mays ubiquitin gene first intron

<220>
<221>  misc_feature
<222>  (6335)..(6886)
<223>  Phosphinothricin Acetyltransferase synthetic gene/CDS, which 
       confers BASTA/PPT resistance

<220>
<221>  terminator
<222>  (7009)..(7619)
<223>  an octapine synthase terminator

<220>
<221>  misc_feature
<222>  (7694)..(7838)
<223>  right T-DNA border from pSB11

<220>
<221>  misc_feature
<222>  (7734)..(7757)
<223>  right T-DNA border repeat from pSB11

<400>  11
gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt     60

gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt    120

actgaattgg atccgcccgg gcggtaccaa gcttccgcgg ctgcagtgca gcgtgacccg    180

gtcgtgcccc tctctagaga taatgagcat tgcatgtcta agttataaaa aattaccaca    240

tatttttttt gtcacacttg tttgaagtgc agtttatcta tctttataca tatatttaaa    300

ctttactcta cgaataatat aatctatagt actacaataa tatcagtgtt ttagagaatc    360

atataaatga acagttagac atggtctaaa ggacaattga gtattttgac aacaggactc    420

tacagtttta tctttttagt gtgcatgtgt tctccttttt ttttgcaaat agcttcacct    480

atataatact tcatccattt tattagtaca tccatttagg gtttagggtt aatggttttt    540

atagactaat ttttttagta catctatttt attctatttt agcctctaaa ttaagaaaac    600

taaaactcta ttttagtttt tttatttaat agtttagata taaaatagaa taaaataaag    660

tgactaaaaa ttaaacaaat accctttaag aaattaaaaa aactaaggaa acatttttct    720

tgtttcgagt agataatgcc agcctgttaa acgccgtcga cgagtctaac ggacaccaac    780

cagcgaacca gcagcgtcgc gtcgggccaa gcgaagcaga cggcacggca tctctgtcgc    840

tgcctctgga cccctctcga gagttccgct ccaccgttgg acttgctccg ctgtcggcat    900

ccagaaattg cgtggcggag cggcagacgt gagccggcac ggcaggcggc ctcctcctcc    960

tctcacggca ccggcagcta cgggggattc ctttcccacc gctccttcgc tttcccttcc   1020

tcgcccgccg taataaatag acaccccctc cacaccctct ttccccaacc tcgtgttgtt   1080

cggagcgcac acacacacaa ccagatctcc cccaaatcca cccgtcggca cctccgcttc   1140

aaggtacgcc gctcgtcctc cccccccccc cccctctcta ccttctctag atcggcgttc   1200

cggtccatgg ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt   1260

tgtgttagat ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt   1320

ctgattgcta acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc   1380

gcagacggga tcgatttcat gatttttttt gtttcgttgc atagggtttg gtttgccctt   1440

ttcctttatt tcaatatatg ccgtgcactt gtttgtcggg tcatcttttc atgctttttt   1500

ttgtcttggt tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtagaattc   1560

tgtttcaaac tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat   1620

tcatagttac gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt   1680

gatgcgggtt ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg   1740

tggtgtggtt gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta   1800

cctggtgtat ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga   1860

gtttaagatg gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact   1920

gatgcatata catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta   1980

tctattataa taaacaagta tgttttataa ttatttcgat cttgatatac ttggatgatg   2040

gcatatgcag cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc   2100

ttggtactgt ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcagggtac   2160

ggatcctcat ctaagcgcaa agagacgtac tatggaaaac gctaaaatga actcgctcat   2220

cgcccagtat ccgttggtaa aggatctggt tgctcttaaa gaaaccacct ggtttaatcc   2280

tggcacgacc tcattggctg aaggtttacc ttatgttggc ctgaccgaac aggatgttca   2340

ggacgcccat gcgcgcttat cccgttttgc accctatctg gcaaaagcat ttcctgaaac   2400

tgctgccact ggggggatta ttgaatcaga actggttgcc attccagcta tgcaaaaacg   2460

gctggaaaaa gaatatcagc aaccgatcag cgggcaactg ttactgaaaa aagatagcca   2520

tttgcccatt tccggctcca taaaagcacg cggcgggatt tatgaagtcc tggcacacgc   2580

agaaaaactg gctctggaag cggggttgct gacgcttgat gatgactaca gcaaactgct   2640

ttctccggag tttaaacagt tctttagcca atacagcatt gctgtgggct caaccggaaa   2700

tctggggtta tcaatcggca ttatgagcgc ccgcattggc tttaaggtga cagttcatat   2760

gtctgctgat gcccgggcat ggaaaaaagc gaaactgcgc agccatggcg ttacggtcgt   2820

ggaatatgag caagattatg gtgttgccgt cgaggaagga cgtaaagcag cgcagtctga   2880

cccgaactgt ttctttattg atgacgaaaa ttcccgcacg ttgttccttg ggtattccgt   2940

cgctggccag cgtcttaaag cgcaatttgc ccagcaaggc cgtatcgtcg atgctgataa   3000

ccctctgttt gtctatctgc cgtgtggtgt tggcggtggt cctggtggcg tcgcattcgg   3060

gcttaaactg gcgtttggcg atcatgttca ctgctttttt gccgaaccaa cgcactcccc   3120

ttgtatgttg ttaggcgtcc atacaggatt acacgatcag atttctgttc aggatattgg   3180

tatcgacaac cttaccgcag cggatggcct tgcagttggt cgcgcatcag gctttgtcgg   3240

gcgggcaatg gagcgtctgc tggatggctt ctataccctt agcgatcaaa ccatgtatga   3300

catgcttggc tggctggcgc aggaagaagg tattcgtctt gaaccttcgg cactggcggg   3360

tatggccgga cctcagcgcg tgtgtgcatc agtaagttac caacagatgc acggtttcag   3420

cgcagaacaa ctgcgtaata ccactcatct ggtgtgggcg acgggaggtg gaatggtgcc   3480

ggaagaagag atgaatcaat atctggcaaa aggccgttaa taacgtttca acgcagcatg   3540

gatcgtaccg agctcaatcg atcctgcttt aatgagatat gcgagacgcc tatgatcgca   3600

tgatatttgc tttcaattct gttgtgcacg ttgtaaaaaa cctgagcatg tgtagctcag   3660

atccttaccg ccggtttcgg ttcattctaa tgaatatatc acccgttact atcgtatttt   3720

tatgaataat attctccgtt caatttactg attgtaccct actacttata tgtacaatat   3780

taaaatgaaa acaatatatt gtgctgaata ggtttatagc gacatctatg atagagcgcc   3840

acaataacaa acaattgcgt tttattatta caaatccaat tttaaaaaaa gcggcagaac   3900

cggtcaaacc taaaagactg attacataaa tcttattcaa atttcaaaag tgccccaggg   3960

gctagtatct acgacacacc gagcggcgaa ctaataacgc tcactgaagg gaactccggt   4020

tccccgccgg cgcgcatggg tgagattcct tgaagttgag tattggccgt ccgctctacc   4080

gaaagttacg ggcaccattc aacccggtcc agcacggcgg ccgggtaacc gacttgctgc   4140

cccgagaatt atgcagcatt tttttggtgt atgtgggccc caaatgaagt gcaggtcaaa   4200

ccttgacagt gacgacaaat cgttgggcgg gtccagggcg aattttgcga caacatgtcg   4260

aggctcagca ggatgggccc aggtacagaa ttcgcggccg taccaagctt ccgcggctgc   4320

agtgcagcgt gacccggtcg tgcccctctc tagagataat gagcattgca tgtctaagtt   4380

ataaaaaatt accacatatt ttttttgtca cacttgtttg aagtgcagtt tatctatctt   4440

tatacatata tttaaacttt actctacgaa taatataatc tatagtacta caataatatc   4500

agtgttttag agaatcatat aaatgaacag ttagacatgg tctaaaggac aattgagtat   4560

tttgacaaca ggactctaca gttttatctt tttagtgtgc atgtgttctc cttttttttt   4620

gcaaatagct tcacctatat aatacttcat ccattttatt agtacatcca tttagggttt   4680

agggttaatg gtttttatag actaattttt ttagtacatc tattttattc tattttagcc   4740

tctaaattaa gaaaactaaa actctatttt agttttttta tttaatagtt tagatataaa   4800

atagaataaa ataaagtgac taaaaattaa acaaataccc tttaagaaat taaaaaaact   4860

aaggaaacat ttttcttgtt tcgagtagat aatgccagcc tgttaaacgc cgtcgacgag   4920

tctaacggac accaaccagc gaaccagcag cgtcgcgtcg ggccaagcga agcagacggc   4980

acggcatctc tgtcgctgcc tctggacccc tctcgagagt tccgctccac cgttggactt   5040

gctccgctgt cggcatccag aaattgcgtg gcggagcggc agacgtgagc cggcacggca   5100

ggcggcctcc tcctcctctc acggcaccgg cagctacggg ggattccttt cccaccgctc   5160

cttcgctttc ccttcctcgc ccgccgtaat aaatagacac cccctccaca ccctctttcc   5220

ccaacctcgt gttgttcgga gcgcacacac acacaaccag atctccccca aatccacccg   5280

tcggcacctc cgcttcaagg tacgccgctc gtcctccccc cccccccccc tctctacctt   5340

ctctagatcg gcgttccggt ccatggttag ggcccggtag ttctacttct gttcatgttt   5400

gtgttagatc cgtgtttgtg ttagatccgt gctgctagcg ttcgtacacg gatgcgacct   5460

gtacgtcaga cacgttctga ttgctaactt gccagtgttt ctctttgggg aatcctggga   5520

tggctctagc cgttccgcag acgggatcga tttcatgatt ttttttgttt cgttgcatag   5580

ggtttggttt gcccttttcc tttatttcaa tatatgccgt gcacttgttt gtcgggtcat   5640

cttttcatgc ttttttttgt cttggttgtg atgatgtggt ctggttgggc ggtcgttcta   5700

gatcggagta gaattctgtt tcaaactacc tggtggattt attaattttg gatctgtatg   5760

tgtgtgccat acatattcat agttacgaat tgaagatgat ggatggaaat atcgatctag   5820

gataggtata catgttgatg cgggttttac tgatgcatat acagagatgc tttttgttcg   5880

cttggttgtg atgatgtggt gtggttgggc ggtcgttcat tcgttctaga tcggagtaga   5940

atactgtttc aaactacctg gtgtatttat taattttgga actgtatgtg tgtgtcatac   6000

atcttcatag ttacgagttt aagatggatg gaaatatcga tctaggatag gtatacatgt   6060

tgatgtgggt tttactgatg catatacatg atggcatatg cagcatctat tcatatgctc   6120

taaccttgag tacctatcta ttataataaa caagtatgtt ttataattat ttcgatcttg   6180

atatacttgg atgatggcat atgcagcagc tatatgtgga tttttttagc cctgccttca   6240

tacgctattt atttgcttgg tactgtttct tttgtcgatg ctcaccctgt tgtttggtgt   6300

tacttctgca gggtacggat cctctagagt cgacatgtct ccggagagga gaccagttga   6360

gattaggcca gctacagcag ccgatatggc cgcggtttgt gacatcgtta accattacat   6420

tgagacgtct acagtgaact ttaggacaga gccacaaaca ccacaagagt ggattgatga   6480

cctagagagg ttgcaagata gatacccttg gttggttgct gaggttgagg gtgttgtggc   6540

tggtattgct tacgctgggc cctggaaggc taggaacgct tacgattgga cagttgagag   6600

tactgtttac gtgtcacata ggcatcaaag gttgggccta ggatctacat tgtacacaca   6660

tttgcttaag tctatggagg cgcaaggttt taagtctgtg gttgctgtta taggccttcc   6720

aaacgatcca tctgttaggt tgcatgaggc tttgggatac acagcgcggg gtacattgcg   6780

cgcggctgga tacaagcatg gtggatggca tgatgttggt ttttggcaaa gggattttga   6840

gttgccagct cctccaaggc cagttaggcc agttacccag atctgagtcg acctgcagga   6900

gctcaatcga tcctgcttta atgagatatg cgagacgcct atgatcgcat gatatttgct   6960

ttcaattctg ttgtgcacgt tgtaaaaaac ctgagcatgt gtagctcaga tccttaccgc   7020

cggtttcggt tcattctaat gaatatatca cccgttacta tcgtattttt atgaataata   7080

ttctccgttc aatttactga ttgtacccta ctacttatat gtacaatatt aaaatgaaaa   7140

caatatattg tgctgaatag gtttatagcg acatctatga tagagcgcca caataacaaa   7200

caattgcgtt ttattattac aaatccaatt ttaaaaaaag cggcagaacc ggtcaaacct   7260

aaaagactga ttacataaat cttattcaaa tttcaaaagt gccccagggg ctagtatcta   7320

cgacacaccg agcggcgaac taataacgct cactgaaggg aactccggtt ccccgccggc   7380

gcgcatgggt gagattcctt gaagttgagt attggccgtc cgctctaccg aaagttacgg   7440

gcaccattca acccggtcca gcacggcggc cgggtaaccg acttgctgcc ccgagaatta   7500

tgcagcattt ttttggtgta tgtgggcccc aaatgaagtg caggtcaaac cttgacagtg   7560

acgacaaatc gttgggcggg tccagggcga attttgcgac aacatgtcga ggctcagcag   7620

gatgggccca ggtacagaat tcgcggccgc ttaattaatc tagaggcgcg ccgggcccgg   7680

ccggccagat cttgattgtc gtttcccgcc ttcagtttaa actatcagtg tttgacagga   7740

tatattggcg ggtaaaccta agagaaaaga gcgtttatta gaataatcgg atatttaaaa   7800

gggcgtgaaa aggtttatcc gttcgtccat ttgtatgt                           7838


<210>  12
<211>  8479
<212>  DNA
<213>  Artificial

<220>
<223>  Nucleic acid sequence encoding T-DNA region of pRLM179, a pRLM175
       derived binary vector containing ZmAHASL2/Xi12 and 
       p-ZmUBI+I::c-dsdA::t-OCS cassettes.


<220>
<221>  misc_feature
<222>  (1)..(128)
<223>  Left T-DNA border from pSB11

<220>
<221>  misc_feature
<222>  (47)..(71)
<223>  Left T-DNA border repeat from pSB11

<220>
<221>  promoter
<222>  (187)..(986)
<223>  AHASL2 [XI12] 5'/UTR promoter for Zea mays gene for 
       acetohydroxyacid synthase (AHAS108)

<220>
<221>  misc_feature
<222>  (987)..(2903)
<223>  AHASL2 [XI12] cds for Zea mays gene for acetohydroxyacid synthase
       (AHAS108) which confers resistance to IMI herbicides.

<220>
<221>  mutation
<222>  (2847)..(2849)
<223>  S621N mutation in AHASL2 [XI12] for Z.mays gene for 
       acetohydroxyacid synthase (AHAS108), confers resistance to IMI 
       herbicides.

<220>
<221>  terminator
<222>  (2902)..(4112)
<223>  AHASL2 [XI12] 3'/UTR for Zea mays gene for acetohydroxyacid 
       synthase (AHAS108).

<220>
<221>  promoter
<222>  (4152)..(6139)
<223>  Zea mays ubiquitin promoter with first intron

<220>
<221>  Intron
<222>  (5134)..(6139)
<223>  Zea mays ubiquitin gene first intron

<220>
<221>  misc_feature
<222>  (6182)..(7510)
<223>  cds encoding E.coli D-serine dehydratase [dsdA]

<220>
<221>  terminator
<222>  (7552)..(8260)
<223>  an octapine synthase terminator

<220>
<221>  misc_feature
<222>  (8335)..(8479)
<223>  right T-DNA border from pSB11

<220>
<221>  misc_feature
<222>  (8375)..(8398)
<223>  right T-DNA border repeat from pSB11

<400>  12
gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt     60

gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt    120

actgaattgg atccgcccgg gcggtacccg gggatcctct agaactagtg gatcccccgg    180

gctgcaggtc aacggatcac ctatcaacat cccagctaaa aacagtaaaa agggggaaaa    240

cgtgggtgag ttgagtctgt cttgtggaaa aaacgtttta gtttctcctg gaattaacaa    300

taaaaacagt tgaacaagat tgactgttcc tccgggaggg tttggaacat cgttacagat    360

gtgagcgaaa ggtgaggaaa cagagcggag ggcttggagg tgacctcggt agtcgacgcc    420

ggagttgagc ttgatgacga caccgtactg gcgtaccagg cctagtagtg aacaccgggc    480

ctgaagctgt cgccgccgct gctcatcttg tgggctgtgc ccggtgtccc tgttgcggat    540

tgcgggtggc agcctggcag gtgggtgcga cccgtttgga ctccctgatc tgggcccttt    600

gtgtcagtac cgtctgtact ccgatgacat gcacaccgtc gtccacagtc aagtccacaa    660

tctcccctct ttttttaacg gaatagttca aaatctcctt gacgcacgct atcgtgtacc    720

agcgctcact ggacaccacg tttgtaatcc acgccgacac gtcggtccca cgtcgacagg    780

ccccaccgtc cggtctgtag cgtgtacgta ttcgggcgac ggacgtgtcg tcgtcgtctt    840

gcgagtccca ttcccatcac catctgagcc acacatcctc tgaacaaaag cagggaggcc    900

tctacgcaca tccccctttc tcccactccg tgtccgtggc acccacccca aaccctcgcg    960

ccgcctccga gacagccgcc gcaaccatgg ccaccgccgc cgccgcgtct accgcgctca   1020

ctggcgccac taccgctgcg cccaaggcga ggcgccgggc gcacctcctg gccacccgcc   1080

gcgccctcgc cgcgcccatc aggtgctcag cggcgtcacc cgccatgccg atggctcccc   1140

cggccacccc gctccggccg tggggcccca ccgatccccg caagggcgcc gacatcctcg   1200

tcgagtccct cgagcgctgc ggcgtccgcg acgtcttcgc ctaccccggc ggcgcgtcca   1260

tggagatcca ccaggcactc acccgctccc ccgtcatcgc caaccacctc ttccgccacg   1320

agcaagggga ggcctttgcg gcctccggct acgcgcgctc ctcgggccgc gtcggcgtct   1380

gcatcgccac ctccggcccc ggcgccacca accttgtctc cgcgctcgcc gacgcgctgc   1440

tcgattccgt ccccatggtc gccatcacgg gacaggtgcc gcgacgcatg attggcaccg   1500

acgccttcca ggagacgccc atcgtcgagg tcacccgctc catcaccaag cacaactacc   1560

tggtcctcga cgtcgacgac atcccccgcg tcgtgcagga ggctttcttc ctcgcctcct   1620

ctggtcgacc ggggccggtg cttgtcgaca tccccaagga catccagcag cagatggcgg   1680

tgcctgtctg ggacaagccc atgagtctgc ctgggtacat tgcgcgcctt cccaagcccc   1740

ctgcgactga gttgcttgag caggtgctgc gtcttgttgg tgaatcccgg cgccctgttc   1800

tttatgttgg cggtggctgc gcagcatctg gtgaggagtt gcgacgcttt gtggagctga   1860

ctggaatccc ggtcacaact actcttatgg gcctcggcaa cttccccagc gacgacccac   1920

tgtctctgcg catgctaggt atgcatggca cggtgtatgc aaattatgca gtggataagg   1980

ccgatctgtt gcttgcactt ggtgtgcggt ttgatgatcg tgtgacaggg aagattgagg   2040

cttttgcaag cagggctaag attgtgcacg ttgatattga tccggctgag attggcaaga   2100

acaagcagcc acatgtgtcc atctgtgcag atgttaagct tgctttgcag ggcatgaatg   2160

ctcttcttga aggaagcaca tcaaagaaga gctttgactt tggctcatgg aacgatgagt   2220

tggatcagca gaagagggaa ttcccccttg ggtataaaac atctaatgag gagatccagc   2280

cacaatatgc tattcaggtt cttgatgagc tgacgaaagg cgaggccatc atcggcacag   2340

gtgttgggca gcaccagatg tgggcggcac agtactacac ttacaagcgg ccaaggcagt   2400

ggttgtcttc agctggtctt ggggctatgg gatttggttt gccggctgct gctggtgctt   2460

ctgtggccaa cccaggtgtt actgttgttg acatcgatgg agatggtagc tttctcatga   2520

acgttcagga gctagctatg atccgaattg agaacctccc ggtgaaggtc tttgtgctaa   2580

acaaccagca cctggggatg gtggtgcagt gggaggacag gttctataag gccaacagag   2640

cgcacacata cttgggaaac ccagagaatg aaagtgagat atatccagat ttcgtgacga   2700

tcgccaaagg gttcaacatt ccagcggtcc gtgtgacaaa gaagaacgaa gtccgcgcag   2760

cgataaagaa gatgctcgag actccagggc cgtacctctt ggatataatc gtcccacacc   2820

aggagcatgt gttgcctatg atccctaatg gtggggcttt caaggatatg atcctggatg   2880

gtgatggcag gactgtgtac tgatctaaaa tccagcaagc aactgatcta aaatccagca   2940

agcaccgcct ccctgctagt acaagggtga tatgttttta tctgtgtgat gttctcctgt   3000

attctatctt tttttgtagg ccgtcagcta tctgttatgg taatcctatg tagcttccga   3060

ccttgtaatt gtgtagtctg ttgttttcct tctggcatgt gtcataagag atcatttaag   3120

tgccttttgc tacatataaa taagataata agcactgcta tgcagtggtt ctgaattggc   3180

ttctgttgcc aaatttaagt gtccaactgg tccttgcttt tgttttcgct atttttttcc   3240

ttttttagtt attattatat tggtaatttc aactcaacat atgatgtatg gaataatgct   3300

agggctgcaa tttcaaacta ttttacaaac cagaatggca ttttcgtggt ttgaggggag   3360

tgaaaaaaaa tgaggcattt gactgaatta gttacctgat ccattttcgt ggtttggatc   3420

attggaatta aattccattc taataatagt aattttggca tatatcaatt aagttaattc   3480

ggttttatgc aaaatatatt tgtatactat tattatcaag atgtcggaga tatttatatg   3540

ctacattttt actatacagg agtgagatga agagtgtcat gtaagttaca cagtagaaac   3600

aaattctatt aatgcataaa atcatttcca tcatccaccc tatgaatttg agatagacct   3660

atatctaaac tttgaaaagt ggttgaatat caaattccaa attaaataag ttattttatt   3720

gagtgaattc taatttctct aaaacgaagg gatctaaacg ccctctaaag ctaatttgga   3780

aactcaaact ttcttagcat tggaggggat tgagaaaaaa tattaattca ttttcatctc   3840

aatcattcaa tctccaaaga gatttgagtt ccttattagt ctgttccatg catcaaatcg   3900

gctcaatgtg tcattatttg ccatgacgat tgacgagttg ttctggggcc tagcgctttc   3960

cacgccgatg tgctggggcc tggtcctgga gaagacagct tgatatttaa agctatcaat   4020

tgtttcaatt gattcccact tcatttttct aaatgtagaa aacggtgacg tataagaaaa   4080

agaatgaatt aggactttta ttccgtacac taatctagag cggccgcaag cttgtaccaa   4140

gcttccgcgg ctgcagtgca gcgtgacccg gtcgtgcccc tctctagaga taatgagcat   4200

tgcatgtcta agttataaaa aattaccaca tatttttttt gtcacacttg tttgaagtgc   4260

agtttatcta tctttataca tatatttaaa ctttactcta cgaataatat aatctatagt   4320

actacaataa tatcagtgtt ttagagaatc atataaatga acagttagac atggtctaaa   4380

ggacaattga gtattttgac aacaggactc tacagtttta tctttttagt gtgcatgtgt   4440

tctccttttt ttttgcaaat agcttcacct atataatact tcatccattt tattagtaca   4500

tccatttagg gtttagggtt aatggttttt atagactaat ttttttagta catctatttt   4560

attctatttt agcctctaaa ttaagaaaac taaaactcta ttttagtttt tttatttaat   4620

agtttagata taaaatagaa taaaataaag tgactaaaaa ttaaacaaat accctttaag   4680

aaattaaaaa aactaaggaa acatttttct tgtttcgagt agataatgcc agcctgttaa   4740

acgccgtcga cgagtctaac ggacaccaac cagcgaacca gcagcgtcgc gtcgggccaa   4800

gcgaagcaga cggcacggca tctctgtcgc tgcctctgga cccctctcga gagttccgct   4860

ccaccgttgg acttgctccg ctgtcggcat ccagaaattg cgtggcggag cggcagacgt   4920

gagccggcac ggcaggcggc ctcctcctcc tctcacggca ccggcagcta cgggggattc   4980

ctttcccacc gctccttcgc tttcccttcc tcgcccgccg taataaatag acaccccctc   5040

cacaccctct ttccccaacc tcgtgttgtt cggagcgcac acacacacaa ccagatctcc   5100

cccaaatcca cccgtcggca cctccgcttc aaggtacgcc gctcgtcctc cccccccccc   5160

cccctctcta ccttctctag atcggcgttc cggtccatgg ttagggcccg gtagttctac   5220

ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat ccgtgctgct agcgttcgta   5280

cacggatgcg acctgtacgt cagacacgtt ctgattgcta acttgccagt gtttctcttt   5340

ggggaatcct gggatggctc tagccgttcc gcagacggga tcgatttcat gatttttttt   5400

gtttcgttgc atagggtttg gtttgccctt ttcctttatt tcaatatatg ccgtgcactt   5460

gtttgtcggg tcatcttttc atgctttttt ttgtcttggt tgtgatgatg tggtctggtt   5520

gggcggtcgt tctagatcgg agtagaattc tgtttcaaac tacctggtgg atttattaat   5580

tttggatctg tatgtgtgtg ccatacatat tcatagttac gaattgaaga tgatggatgg   5640

aaatatcgat ctaggatagg tatacatgtt gatgcgggtt ttactgatgc atatacagag   5700

atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt gggcggtcgt tcattcgttc   5760

tagatcggag tagaatactg tttcaaacta cctggtgtat ttattaattt tggaactgta   5820

tgtgtgtgtc atacatcttc atagttacga gtttaagatg gatggaaata tcgatctagg   5880

ataggtatac atgttgatgt gggttttact gatgcatata catgatggca tatgcagcat   5940

ctattcatat gctctaacct tgagtaccta tctattataa taaacaagta tgttttataa   6000

ttatttcgat cttgatatac ttggatgatg gcatatgcag cagctatatg tggatttttt   6060

tagccctgcc ttcatacgct atttatttgc ttggtactgt ttcttttgtc gatgctcacc   6120

ctgttgtttg gtgttacttc tgcagggtac ggatcctcat ctaagcgcaa agagacgtac   6180

tatggaaaac gctaaaatga actcgctcat cgcccagtat ccgttggtaa aggatctggt   6240

tgctcttaaa gaaaccacct ggtttaatcc tggcacgacc tcattggctg aaggtttacc   6300

ttatgttggc ctgaccgaac aggatgttca ggacgcccat gcgcgcttat cccgttttgc   6360

accctatctg gcaaaagcat ttcctgaaac tgctgccact ggggggatta ttgaatcaga   6420

actggttgcc attccagcta tgcaaaaacg gctggaaaaa gaatatcagc aaccgatcag   6480

cgggcaactg ttactgaaaa aagatagcca tttgcccatt tccggctcca taaaagcacg   6540

cggcgggatt tatgaagtcc tggcacacgc agaaaaactg gctctggaag cggggttgct   6600

gacgcttgat gatgactaca gcaaactgct ttctccggag tttaaacagt tctttagcca   6660

atacagcatt gctgtgggct caaccggaaa tctggggtta tcaatcggca ttatgagcgc   6720

ccgcattggc tttaaggtga cagttcatat gtctgctgat gcccgggcat ggaaaaaagc   6780

gaaactgcgc agccatggcg ttacggtcgt ggaatatgag caagattatg gtgttgccgt   6840

cgaggaagga cgtaaagcag cgcagtctga cccgaactgt ttctttattg atgacgaaaa   6900

ttcccgcacg ttgttccttg ggtattccgt cgctggccag cgtcttaaag cgcaatttgc   6960

ccagcaaggc cgtatcgtcg atgctgataa ccctctgttt gtctatctgc cgtgtggtgt   7020

tggcggtggt cctggtggcg tcgcattcgg gcttaaactg gcgtttggcg atcatgttca   7080

ctgctttttt gccgaaccaa cgcactcccc ttgtatgttg ttaggcgtcc atacaggatt   7140

acacgatcag atttctgttc aggatattgg tatcgacaac cttaccgcag cggatggcct   7200

tgcagttggt cgcgcatcag gctttgtcgg gcgggcaatg gagcgtctgc tggatggctt   7260

ctataccctt agcgatcaaa ccatgtatga catgcttggc tggctggcgc aggaagaagg   7320

tattcgtctt gaaccttcgg cactggcggg tatggccgga cctcagcgcg tgtgtgcatc   7380

agtaagttac caacagatgc acggtttcag cgcagaacaa ctgcgtaata ccactcatct   7440

ggtgtgggcg acgggaggtg gaatggtgcc ggaagaagag atgaatcaat atctggcaaa   7500

aggccgttaa taacgtttca acgcagcatg gatcgtaccg agctcaatcg atcctgcttt   7560

aatgagatat gcgagacgcc tatgatcgca tgatatttgc tttcaattct gttgtgcacg   7620

ttgtaaaaaa cctgagcatg tgtagctcag atccttaccg ccggtttcgg ttcattctaa   7680

tgaatatatc acccgttact atcgtatttt tatgaataat attctccgtt caatttactg   7740

attgtaccct actacttata tgtacaatat taaaatgaaa acaatatatt gtgctgaata   7800

ggtttatagc gacatctatg atagagcgcc acaataacaa acaattgcgt tttattatta   7860

caaatccaat tttaaaaaaa gcggcagaac cggtcaaacc taaaagactg attacataaa   7920

tcttattcaa atttcaaaag tgccccaggg gctagtatct acgacacacc gagcggcgaa   7980

ctaataacgc tcactgaagg gaactccggt tccccgccgg cgcgcatggg tgagattcct   8040

tgaagttgag tattggccgt ccgctctacc gaaagttacg ggcaccattc aacccggtcc   8100

agcacggcgg ccgggtaacc gacttgctgc cccgagaatt atgcagcatt tttttggtgt   8160

atgtgggccc caaatgaagt gcaggtcaaa ccttgacagt gacgacaaat cgttgggcgg   8220

gtccagggcg aattttgcga caacatgtcg aggctcagca ggatgggccc aggtacagaa   8280

ttcgcggccg cttaattaat ctagaggcgc gccgggcccg gccggccaga tcttgattgt   8340

cgtttcccgc cttcagttta aactatcagt gtttgacagg atatattggc gggtaaacct   8400

aagagaaaag agcgtttatt agaataatcg gatatttaaa agggcgtgaa aaggtttatc   8460

cgttcgtcca tttgtatgt                                                8479


<210>  13
<211>  8017
<212>  DNA
<213>  Artificial

<220>
<223>  Nucleic acid sequence encoding T-DNA region of pRLM205, a pRLM175
       derived binary vector containing p-ZmUBI+I::c-dao1::t-OCS and 
       p-ScBV::c-gusINT::t-NOS cassettes.


<220>
<221>  misc_feature
<222>  (1)..(128)
<223>  Left T-DNA border from pSB11

<220>
<221>  misc_feature
<222>  (47)..(71)
<223>  Left T-DNA border repeat from pSB11

<220>
<221>  promoter
<222>  (162)..(2149)
<223>  Zea mays ubiquitin promoter with first intron

<220>
<221>  Intron
<222>  (1144)..(2149)
<223>  Zea mays ubiquitin gene first intron

<220>
<221>  misc_feature
<222>  (2192)..(3298)
<223>  cds encoding Rhodosporidium toruloides D-amino acid oxidase 
       (dao1)

<220>
<221>  terminator
<222>  (3227)..(4035)
<223>  an octapine synthase terminator

<220>
<221>  promoter
<222>  (4094)..(5491)
<223>  Sugarcane bacilliform virus [ScBV] promoter

<220>
<221>  misc_feature
<222>  (5523)..(7520)
<223>  gusINT cds and intron

<220>
<221>  Intron
<222>  (5908)..(6096)
<223>  PIV2 intron of gusINT, a modified potato ST-LS1 intron

<220>
<221>  terminator
<222>  (7591)..(7843)
<223>  3'UTR of Nopaline Synthase from Agrobacterium tumefaciens T-DNA 
       used as terminator

<220>
<221>  misc_feature
<222>  (7873)..(8017)
<223>  right T-DNA border from pSB11

<220>
<221>  misc_feature
<222>  (7913)..(7936)
<223>  right T-DNA border repeat from pSB11

<400>  13
gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt     60

gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt    120

actgaattgg atccgcccgg gcggtaccaa gcttccgcgg ctgcagtgca gcgtgacccg    180

gtcgtgcccc tctctagaga taatgagcat tgcatgtcta agttataaaa aattaccaca    240

tatttttttt gtcacacttg tttgaagtgc agtttatcta tctttataca tatatttaaa    300

ctttactcta cgaataatat aatctatagt actacaataa tatcagtgtt ttagagaatc    360

atataaatga acagttagac atggtctaaa ggacaattga gtattttgac aacaggactc    420

tacagtttta tctttttagt gtgcatgtgt tctccttttt ttttgcaaat agcttcacct    480

atataatact tcatccattt tattagtaca tccatttagg gtttagggtt aatggttttt    540

atagactaat ttttttagta catctatttt attctatttt agcctctaaa ttaagaaaac    600

taaaactcta ttttagtttt tttatttaat agtttagata taaaatagaa taaaataaag    660

tgactaaaaa ttaaacaaat accctttaag aaattaaaaa aactaaggaa acatttttct    720

tgtttcgagt agataatgcc agcctgttaa acgccgtcga cgagtctaac ggacaccaac    780

cagcgaacca gcagcgtcgc gtcgggccaa gcgaagcaga cggcacggca tctctgtcgc    840

tgcctctgga cccctctcga gagttccgct ccaccgttgg acttgctccg ctgtcggcat    900

ccagaaattg cgtggcggag cggcagacgt gagccggcac ggcaggcggc ctcctcctcc    960

tctcacggca ccggcagcta cgggggattc ctttcccacc gctccttcgc tttcccttcc   1020

tcgcccgccg taataaatag acaccccctc cacaccctct ttccccaacc tcgtgttgtt   1080

cggagcgcac acacacacaa ccagatctcc cccaaatcca cccgtcggca cctccgcttc   1140

aaggtacgcc gctcgtcctc cccccccccc cccctctcta ccttctctag atcggcgttc   1200

cggtccatgg ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt   1260

tgtgttagat ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt   1320

ctgattgcta acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc   1380

gcagacggga tcgatttcat gatttttttt gtttcgttgc atagggtttg gtttgccctt   1440

ttcctttatt tcaatatatg ccgtgcactt gtttgtcggg tcatcttttc atgctttttt   1500

ttgtcttggt tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtagaattc   1560

tgtttcaaac tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat   1620

tcatagttac gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt   1680

gatgcgggtt ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg   1740

tggtgtggtt gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta   1800

cctggtgtat ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga   1860

gtttaagatg gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact   1920

gatgcatata catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta   1980

tctattataa taaacaagta tgttttataa ttatttcgat cttgatatac ttggatgatg   2040

gcatatgcag cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc   2100

ttggtactgt ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcagggtac   2160

ggatccggcg cgccactagt cccgggccac catgcactcg cagaagcgcg tcgttgtcct   2220

cggatcaggc gttatcggtc tgagcagcgc cctcatcctc gctcggaagg gctacagcgt   2280

gcatattctc gcgcgcgact tgccggagga cgtctcgagc cagactttcg cttcaccatg   2340

ggctggcgcg aattggacgc ctttcatgac gcttacagac ggtcctcgac aagcaaaatg   2400

ggaagaatcg actttcaaga agtgggtcga gttggtcccg acgggccatg ccatgtggct   2460

caaggggacg aggcggttcg cgcagaacga agacggcttg ctcgggcact ggtacaagga   2520

catcacgcca aattaccgcc ccctcccatc ttccgaatgt ccacctggcg ctatcggcgt   2580

aacctacgac accctctccg tccacgcacc aaagtactgc cagtaccttg caagagagct   2640

gcagaagctc ggcgcgacgt ttgagagacg gaccgttacg tcgcttgagc aggcgttcga   2700

cggtgcggat ttggtggtca acgctacggg acttggcgcc aagtcgattg cgggcatcga   2760

cgaccaagcc gccgagccaa tccgcgggca aaccgtcctc gtcaagtccc catgcaagcg   2820

atgcacgatg gactcgtccg accccgcttc tcccgcctac atcattcccc gaccaggtgg   2880

cgaagtcatc tgcggcggga cgtacggcgt gggagactgg gacttgtctg tcaacccaga   2940

gacggtccag cggatcctca agcactgctt gcgcctcgac ccgaccatct cgagcgacgg   3000

aacgatcgaa ggcatcgagg tcctccgcca caacgtcggc ttgcgacctg cacgacgagg   3060

cggaccccgc gttgaggcag aacggatcgt cctgcctctc gaccggacaa agtcgcccct   3120

ctcgctcggc aggggcagcg cacgagcggc gaaggagaag gaggtcacgc ttgtgcatgc   3180

gtatggcttc tcgagtgcgg gataccagca gagttggggc gcggcggagg atgtcgcgca   3240

gctcgtcgac gaggcgttcc agcggtacca cggcgcggcg cgggagtcga agttgtaggg   3300

cgggattcct gcaggagctc aatcgatcct gctttaatga gatatgcgag acgcctatga   3360

tcgcatgata tttgctttca attctgttgt gcacgttgta aaaaacctga gcatgtgtag   3420

ctcagatcct taccgccggt ttcggttcat tctaatgaat atatcacccg ttactatcgt   3480

atttttatga ataatattct ccgttcaatt tactgattgt accctactac ttatatgtac   3540

aatattaaaa tgaaaacaat atattgtgct gaataggttt atagcgacat ctatgataga   3600

gcgccacaat aacaaacaat tgcgttttat tattacaaat ccaattttaa aaaaagcggc   3660

agaaccggtc aaacctaaaa gactgattac ataaatctta ttcaaatttc aaaagtgccc   3720

caggggctag tatctacgac acaccgagcg gcgaactaat aacgctcact gaagggaact   3780

ccggttcccc gccggcgcgc atgggtgaga ttccttgaag ttgagtattg gccgtccgct   3840

ctaccgaaag ttacgggcac cattcaaccc ggtccagcac ggcggccggg taaccgactt   3900

gctgccccga gaattatgca gcattttttt ggtgtatgtg ggccccaaat gaagtgcagg   3960

tcaaaccttg acagtgacga caaatcgttg ggcgggtcca gggcgaattt tgcgacaaca   4020

tgtcgaggct cagcaggatg ggcccaggta cagaattcgc ggccgcttaa ttaaggtacc   4080

caattgcata tgtaatcctg gctagcaaca ctgaactatg ccagaaacca catcaaagat   4140

atgggcaagc ttcttggccc attatatcca aagacctcag agaaaggtga gcgaaggctc   4200

aattcagaag attggaagct gatcaatagg atcaagacaa tggtgagaac gcttccaaat   4260

ctcactattc caccagaaga tgcatacatt atcattgaaa cagatgcatg tgcaactgga   4320

tggggagcag tatgcaagtg gaagaaaaac aaggcagacc caagaaatac agagcaaatc   4380

tgtaggtatg ccagtggaaa atttgataag ccaaaaggaa cctgtgatgc agaaatctat   4440

ggggttatga atggcttaga aaagatgaga ttgttctact tggacaaaag agagatcaca   4500

gtcagaactg acagtagtgc aatcgaaagg ttctacaaca agagtgctga acacaagcct   4560

tctgagatca gatggatcag gttcatggac tacatcactg gtgcaggacc agagatagtc   4620

attgaacaca taaaagggaa gagcaatggt ttagctgaca tcttgtccag gctcaaagcc   4680

aaattagctc agaatgaacc aacggaagag atgatcctgc ttacacaagc cataagggaa   4740

gtaattcctt atccagatca tccatacact gagcaactca gagaatgggg aaacaaaatt   4800

ctggatccat tccccacatt caagaaggac atgttcgaaa gaacagagca agcttttatg   4860

ctaacagagg aaccagttct actctgtgca tgcaggaagc ctgcaattca gttagtgtcc   4920

agaacatctg ccaacccagg aaggaaattc ttcaagtgcg caatgaacaa atgccattgc   4980

tggtactggg cagatctcat tgaagaacac attcaagaca gaattgatga atttctcaag   5040

aatcttgaag ttctgaagac cggtggcgtg caaacaatgg aggaggaact tatgaaggaa   5100

gtcaccaagc tgaagataga agagcaggag ttcgaggaat accaggccac accaagggct   5160

atgtcgccag tagccgcaga agatgtgcta gatctccaag acgtaagcaa tgacgattga   5220

ggaggcattg acgtcaggga tgaccgcagc ggagagtact gggcccattc agtggatgct   5280

ccactgagtt gtattattgt gtgcttttcg gacaagtgtg ctgtccactt tcttttggca   5340

cctgtgccac tttattcctt gtctgccacg atgcctttgc ttagcttgta agcaaggatc   5400

gcagtgcgtg tgtgacacca ccccccttcc gacgctctgc ctatataagg caccgtctgt   5460

aagctcttac gatcatcggt agttcaccaa gggggtacga tctccgggta ggtcagtccc   5520

ttatgttacg tcctgtagaa accccaaccc gtgaaatcaa aaaactcgac ggcctgtggg   5580

cattcagtct ggatcgcgaa aactgtggaa ttgatcagcg ttggtgggaa agcgcgttac   5640

aagaaagccg ggcaattgct gtgccaggca gttttaacga tcagttcgcc gatgcagata   5700

ttcgtaatta tgcgggcaac gtctggtatc agcgcgaagt ctttataccg aaaggttggg   5760

caggccagcg tatcgtgctg cgtttcgatg cggtcactca ttacggcaaa gtgtgggtca   5820

ataatcagga agtgatggag catcagggcg gctatacgcc atttgaagcc gatgtcacgc   5880

cgtatgttat tgccgggaaa agtgtacgta agtttctgct tctacctttg atatatatat   5940

aataattatc attaattagt agtaatataa tatttcaaat atttttttca aaataaaaga   6000

atgtagtata tagcaattgc ttttctgtag tttataagtg tgtatatttt aatttataac   6060

ttttctaata tatgaccaaa atttgttgat gtgcaggtat caccgtttgt gtgaacaacg   6120

aactgaactg gcagactatc ccgccgggaa tggtgattac cgacgaaaac ggcaagaaaa   6180

agcagtctta cttccatgat ttctttaact atgccggaat ccatcgcagc gtaatgctct   6240

acaccacgcc gaacacctgg gtggacgata tcaccgtggt gacgcatgtc gcgcaagact   6300

gtaaccacgc gtctgttgac tggcaggtgg tggccaatgg tgatgtcagc gttgaactgc   6360

gtgatgcgga tcaacaggtg gttgcaactg gacaaggcac tagcgggact ttgcaagtgg   6420

tgaatccgca cctctggcaa ccgggtgaag gttatctcta tgaactgtgc gtcacagcca   6480

aaagccagac agagtgtgat atctacccgc ttcgcgtcgg catccggtca gtggcagtga   6540

agggccaaca gttcctgatt aaccacaaac cgttctactt tactggcttt ggtcgtcatg   6600

aagatgcgga cttacgtggc aaaggattcg ataacgtgct gatggtgcac gaccacgcat   6660

taatggactg gattggggcc aactcctacc gtacctcgca ttacccttac gctgaagaga   6720

tgctcgactg ggcagatgaa catggcatcg tggtgattga tgaaactgct gctgtcggct   6780

ttaacctctc tttaggcatt ggtttcgaag cgggcaacaa gccgaaagaa ctgtacagcg   6840

aagaggcagt caacggggaa actcagcaag cgcacttaca ggcgattaaa gagctgatag   6900

cgcgtgacaa aaaccaccca agcgtggtga tgtggagtat tgccaacgaa ccggataccc   6960

gtccgcaagt gcacgggaat atttcgccac tggcggaagc aacgcgtaaa ctcgacccga   7020

cgcgtccgat cacctgcgtc aatgtaatgt tctgcgacgc tcacaccgat accatcagcg   7080

atctctttga tgtgctgtgc ctgaaccgtt attacggatg gtatgtccaa agcggcgatt   7140

tggaaacggc agagaaggta ctggaaaaag aacttctggc ctggcaggag aaactgcatc   7200

agccgattat catcaccgaa tacggcgtgg atacgttagc cgggctgcac tcaatgtaca   7260

ccgacatgtg gagtgaagag tatcagtgtg catggctgga tatgtatcac cgcgtctttg   7320

atcgcgtcag cgccgtcgtc ggtgaacagg tatggaattt cgccgatttt gcgacctcgc   7380

aaggcatatt gcgcgttggc ggtaacaaga aagggatctt cactcgcgac cgcaaaccga   7440

agtcggcggc ttttctgctg caaaaacgct ggactggcat gaacttcggt gaaaaaccgc   7500

agcagggagg caaacaatga atcaacaact ctcctggcgc accatcgtcg gctacagcct   7560

cgggaattgc taccgagctc gaatttcccc gatcgttcaa acatttggca ataaagtttc   7620

ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac   7680

gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg   7740

attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac   7800

taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgggaatt cgggcccggc   7860

cggccagatc ttgattgtcg tttcccgcct tcagtttaaa ctatcagtgt ttgacaggat   7920

atattggcgg gtaaacctaa gagaaaagag cgtttattag aataatcgga tatttaaaag   7980

ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgt                            8017


<210>  14
<211>  8102
<212>  DNA
<213>  Artificial

<220>
<223>  Nucleic acid sequence encoding T-DNA region of pRLM226, a pRLM175
       derived binary vector containing 
       p-ZmUBI+I::l-PsFed1::c-dao1/ko::t-OCS and p-ScBV::c-gusINT::t-NOS
       cas-settes.


<220>
<221>  misc_feature
<222>  (1)..(128)
<223>  Left T-DNA border from pSB11

<220>
<221>  misc_feature
<222>  (47)..(71)
<223>  Left T-DNA border repeat from pSB11

<220>
<221>  promoter
<222>  (162)..(2149)
<223>  Zea mays ubiquitin promoter with first intron

<220>
<221>  Intron
<222>  (1144)..(2149)
<223>  Zea mays ubiquitin gene first intron

<220>
<221>  misc_feature
<222>  (2167)..(2256)
<223>  leader fragment for pea Fed1 gene

<220>
<221>  misc_feature
<222>  (2280)..(3386)
<223>  cds encoding a codon optimize, to Zea mays, Rhodosporidium 
       toruloides D-amino acid oxidase (dao1/ko)

<220>
<221>  terminator
<222>  (3409)..(4117)
<223>  an octapine synthase terminator

<220>
<221>  promoter
<222>  (4176)..(5573)
<223>  Sugarcane bacilliform virus [ScBV] promoter

<220>
<221>  misc_feature
<222>  (5605)..(7605)
<223>  gusINT cds and intron

<220>
<221>  Intron
<222>  (5990)..(6178)
<223>  PIV2 intron of gusINT, a modified potato ST-LS1 intron

<220>
<221>  terminator
<222>  (7676)..(7928)
<223>  3'UTR of Nopaline Synthase from Agrobacterium tumefaciens T-DNA 
       used as terminator

<220>
<221>  misc_feature
<222>  (7958)..(8102)
<223>  right T-DNA border from pSB11

<220>
<221>  misc_feature
<222>  (7998)..(8021)
<223>  right T-DNA border repeat from pSB11

<400>  14
gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt     60

gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt    120

actgaattgg atccgcccgg gcggtaccaa gcttccgcgg ctgcagtgca gcgtgacccg    180

gtcgtgcccc tctctagaga taatgagcat tgcatgtcta agttataaaa aattaccaca    240

tatttttttt gtcacacttg tttgaagtgc agtttatcta tctttataca tatatttaaa    300

ctttactcta cgaataatat aatctatagt actacaataa tatcagtgtt ttagagaatc    360

atataaatga acagttagac atggtctaaa ggacaattga gtattttgac aacaggactc    420

tacagtttta tctttttagt gtgcatgtgt tctccttttt ttttgcaaat agcttcacct    480

atataatact tcatccattt tattagtaca tccatttagg gtttagggtt aatggttttt    540

atagactaat ttttttagta catctatttt attctatttt agcctctaaa ttaagaaaac    600

taaaactcta ttttagtttt tttatttaat agtttagata taaaatagaa taaaataaag    660

tgactaaaaa ttaaacaaat accctttaag aaattaaaaa aactaaggaa acatttttct    720

tgtttcgagt agataatgcc agcctgttaa acgccgtcga cgagtctaac ggacaccaac    780

cagcgaacca gcagcgtcgc gtcgggccaa gcgaagcaga cggcacggca tctctgtcgc    840

tgcctctgga cccctctcga gagttccgct ccaccgttgg acttgctccg ctgtcggcat    900

ccagaaattg cgtggcggag cggcagacgt gagccggcac ggcaggcggc ctcctcctcc    960

tctcacggca ccggcagcta cgggggattc ctttcccacc gctccttcgc tttcccttcc   1020

tcgcccgccg taataaatag acaccccctc cacaccctct ttccccaacc tcgtgttgtt   1080

cggagcgcac acacacacaa ccagatctcc cccaaatcca cccgtcggca cctccgcttc   1140

aaggtacgcc gctcgtcctc cccccccccc cccctctcta ccttctctag atcggcgttc   1200

cggtccatgg ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt   1260

tgtgttagat ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt   1320

ctgattgcta acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc   1380

gcagacggga tcgatttcat gatttttttt gtttcgttgc atagggtttg gtttgccctt   1440

ttcctttatt tcaatatatg ccgtgcactt gtttgtcggg tcatcttttc atgctttttt   1500

ttgtcttggt tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtagaattc   1560

tgtttcaaac tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat   1620

tcatagttac gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt   1680

gatgcgggtt ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg   1740

tggtgtggtt gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta   1800

cctggtgtat ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga   1860

gtttaagatg gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact   1920

gatgcatata catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta   1980

tctattataa taaacaagta tgttttataa ttatttcgat cttgatatac ttggatgatg   2040

gcatatgcag cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc   2100

ttggtactgt ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcagggtac   2160

ggatctctcc ttatttcatt cattcattca ttctctatct ttttatcatc aacacaaaca   2220

caaaacagtg tttgttcctt tgaaaccata atagtaggcg cgccggatcc cgggccacca   2280

tgcactctca aaaacgcgtt gtagtattgg gttcgggagt cataggtctt tcttctgctc   2340

ttatacttgc acgcaagggc tattctgttc atattttggc acgcgatctc ccagaggacg   2400

tatctagcca aactttcgct tccccctggg ctggtgcgaa ttggacacca tttatgacat   2460

tgactgacgg ccctagacag gcaaaatggg aagagagtac ctttaaaaaa tgggttgagt   2520

tggtccctac cggacatgct atgtggctga agggaactcg tcgctttgca cagaatgaag   2580

acggcttgct tggacattgg tacaaagata taaccccgaa ttaccgcccc cttccctcgt   2640

ctgaatgtcc accaggcgca attggcgtga cttatgacac actctctgta cacgctccta   2700

aatattgtca gtatcttgcc agagaacttc agaaactcgg tgcaactttt gaaagacgga   2760

cggttacctc actggaacag gcattcgacg gtgcagacct cgtggtgaat gcaacaggac   2820

tgggagcaaa gagcatagca ggcatagatg atcaagctgc ggagccaatt agaggccaga   2880

ccgtgctggt aaaatcacca tgcaaaagat gtacgatgga tagtagtgac ccagcgagtc   2940

cagcttatat tataccgaga ccgggtggcg aagttatatg cggaggaact tacggagtgg   3000

gtgattggga tcttagcgtt aaccccgaaa cagtccaacg gattctgaag cattgcctcc   3060

gccttgaccc aaccatatca tctgacggaa ccattgaggg aatcgaagta cttaggcata   3120

atgttggtct taggcccgct cgtagaggag gaccacgtgt agaagctgag agaattgtat   3180

tgcccctcga tagaaccaaa agcccccttt cgcttggaag aggaagcgca cgtgcagcta   3240

aggaaaaaga agtcacactg gtacatgcat atggttttag tagtgccgga tatcagcaat   3300

catggggtgc tgcagaagat gttgctcagt tggtggacga ggcattccaa agataccacg   3360

gtgcagcgag agagtcaaaa ctttagtgac ctgcaggagc tcaatcgatc ctgctttaat   3420

gagatatgcg agacgcctat gatcgcatga tatttgcttt caattctgtt gtgcacgttg   3480

taaaaaacct gagcatgtgt agctcagatc cttaccgccg gtttcggttc attctaatga   3540

atatatcacc cgttactatc gtatttttat gaataatatt ctccgttcaa tttactgatt   3600

gtaccctact acttatatgt acaatattaa aatgaaaaca atatattgtg ctgaataggt   3660

ttatagcgac atctatgata gagcgccaca ataacaaaca attgcgtttt attattacaa   3720

atccaatttt aaaaaaagcg gcagaaccgg tcaaacctaa aagactgatt acataaatct   3780

tattcaaatt tcaaaagtgc cccaggggct agtatctacg acacaccgag cggcgaacta   3840

ataacgctca ctgaagggaa ctccggttcc ccgccggcgc gcatgggtga gattccttga   3900

agttgagtat tggccgtccg ctctaccgaa agttacgggc accattcaac ccggtccagc   3960

acggcggccg ggtaaccgac ttgctgcccc gagaattatg cagcattttt ttggtgtatg   4020

tgggccccaa atgaagtgca ggtcaaacct tgacagtgac gacaaatcgt tgggcgggtc   4080

cagggcgaat tttgcgacaa catgtcgagg ctcagcagga tgggcccagg tacagaattc   4140

gcggccgctt aattaaggta cccaattgca tatgtaatcc tggctagcaa cactgaacta   4200

tgccagaaac cacatcaaag atatgggcaa gcttcttggc ccattatatc caaagacctc   4260

agagaaaggt gagcgaaggc tcaattcaga agattggaag ctgatcaata ggatcaagac   4320

aatggtgaga acgcttccaa atctcactat tccaccagaa gatgcataca ttatcattga   4380

aacagatgca tgtgcaactg gatggggagc agtatgcaag tggaagaaaa acaaggcaga   4440

cccaagaaat acagagcaaa tctgtaggta tgccagtgga aaatttgata agccaaaagg   4500

aacctgtgat gcagaaatct atggggttat gaatggctta gaaaagatga gattgttcta   4560

cttggacaaa agagagatca cagtcagaac tgacagtagt gcaatcgaaa ggttctacaa   4620

caagagtgct gaacacaagc cttctgagat cagatggatc aggttcatgg actacatcac   4680

tggtgcagga ccagagatag tcattgaaca cataaaaggg aagagcaatg gtttagctga   4740

catcttgtcc aggctcaaag ccaaattagc tcagaatgaa ccaacggaag agatgatcct   4800

gcttacacaa gccataaggg aagtaattcc ttatccagat catccataca ctgagcaact   4860

cagagaatgg ggaaacaaaa ttctggatcc attccccaca ttcaagaagg acatgttcga   4920

aagaacagag caagctttta tgctaacaga ggaaccagtt ctactctgtg catgcaggaa   4980

gcctgcaatt cagttagtgt ccagaacatc tgccaaccca ggaaggaaat tcttcaagtg   5040

cgcaatgaac aaatgccatt gctggtactg ggcagatctc attgaagaac acattcaaga   5100

cagaattgat gaatttctca agaatcttga agttctgaag accggtggcg tgcaaacaat   5160

ggaggaggaa cttatgaagg aagtcaccaa gctgaagata gaagagcagg agttcgagga   5220

ataccaggcc acaccaaggg ctatgtcgcc agtagccgca gaagatgtgc tagatctcca   5280

agacgtaagc aatgacgatt gaggaggcat tgacgtcagg gatgaccgca gcggagagta   5340

ctgggcccat tcagtggatg ctccactgag ttgtattatt gtgtgctttt cggacaagtg   5400

tgctgtccac tttcttttgg cacctgtgcc actttattcc ttgtctgcca cgatgccttt   5460

gcttagcttg taagcaagga tcgcagtgcg tgtgtgacac cacccccctt ccgacgctct   5520

gcctatataa ggcaccgtct gtaagctctt acgatcatcg gtagttcacc aagggggtac   5580

gatctccggg taggtcagtc ccttatgtta cgtcctgtag aaaccccaac ccgtgaaatc   5640

aaaaaactcg acggcctgtg ggcattcagt ctggatcgcg aaaactgtgg aattggtcag   5700

cgttggtggg aaagcgcgtt acaagaaagc cgggcaattg ctgtgccagg cagttttaac   5760

gatcagttcg ccgatgcaga tattcgtaat tatgcgggca acgtctggta tcagcgcgaa   5820

gtctttatac cgaaaggttg ggcaggccag cgtatcgtgc tgcgtttcga tgcggtcact   5880

cattacggca aagtgtgggt caataatcag gaagtgatgg agcatcaggg cggctatacg   5940

ccatttgaag ccgatgtcac gccgtatgtt attgccggga aaagtgtacg taagtttctg   6000

cttctacctt tgatatatat ataataatta tcattaatta gtagtaatat aatatttcaa   6060

atattttttt caaaataaaa gaatgtagta tatagcaatt gcttttctgt agtttataag   6120

tgtgtatatt ttaatttata acttttctaa tatatgacca aaatttgttg atgtgcaggt   6180

atcaccgttt gtgtgaacaa cgaactgaac tggcagacta tcccgccggg aatggtgatt   6240

accgacgaaa acggcaagaa aaagcagtct tacttccatg atttctttaa ctatgccgga   6300

atccatcgca gcgtaatgct ctacaccacg ccgaacacct gggtggacga tatcaccgtg   6360

gtgacgcatg tcgcgcaaga ctgtaaccac gcgtctgttg actggcaggt ggtggccaat   6420

ggtgatgtca gcgttgaact gcgtgatgcg gatcaacagg tggttgcaac tggacaaggc   6480

actagcggga ctttgcaagt ggtgaatccg cacctctggc aaccgggtga aggttatctc   6540

tatgaactgt gcgtcacagc caaaagccag acagagtgtg atatctaccc gcttcgcgtc   6600

ggcatccggt cagtggcagt gaagggcgaa cagttcctga ttaaccacaa accgttctac   6660

tttactggct ttggtcgtca tgaagatgcg gacttgcgtg gcaaaggatt cgataacgtg   6720

ctgatggtgc acgaccacgc attaatggac tggattgggg ccaactccta ccgtacctcg   6780

cattaccctt acgctgaaga gatgctcgac tgggcagatg aacatggcat cgtggtgatt   6840

gatgaaactg ctgctgtcgg ctttaacctc tctttaggca ttggtttcga agcgggcaac   6900

aagccgaaag aactgtacag cgaagaggca gtcaacgggg aaactcagca agcgcactta   6960

caggcgatta aagagctgat agcgcgtgac aaaaaccacc caagcgtggt gatgtggagt   7020

attgccaacg aaccggatac ccgtccgcaa ggtgcacggg aatatttcgc gccactggcg   7080

gaagcaacgc gtaaactcga cccgacgcgt ccgatcacct gcgtcaatgt aatgttctgc   7140

gacgctcaca ccgataccat cagcgatctc tttgatgtgc tgtgcctgaa ccgttattac   7200

ggatggtatg tccaaagcgg cgatttggaa acggcagaga aggtactgga aaaagaactt   7260

ctggcctggc aggagaaact gcatcagccg attatcatca ccgaatacgg cgtggatacg   7320

ttagccgggc tgcactcaat gtacaccgac atgtggagtg aagagtatca gtgtgcatgg   7380

ctggatatgt atcaccgcgt ctttgatcgc gtcagcgccg tcgtcggtga acaggtatgg   7440

aatttcgccg attttgcgac ctcgcaaggc atattgcgcg ttggcggtaa caagaaaggg   7500

atcttcactc gcgaccgcaa accgaagtcg gcggcttttc tgctgcaaaa acgctggact   7560

ggcatgaact tcggtgaaaa accgcagcag ggaggcaaac aatgaatcaa caactctcct   7620

ggcgcaccat cgtcggctac agcctcggga attgctaccg agctcgaatt tccccgatcg   7680

ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat   7740

tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac   7800

gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat   7860

agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt   7920

actagatcgg gaattcgggc ccggccggcc agatcttgat tgtcgtttcc cgccttcagt   7980

ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt   8040

attagaataa tcggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat   8100

gt                                                                  8102


<210>  15
<211>  1107
<212>  DNA
<213>  Rhodosporidium toruloides


<220>
<221>  CDS
<222>  (1)..(1107)
<223>  Zea mays codon optimize Rhodosporidium toruloides D-amino acid 
       oxidase CDS

<400>  15
atg cac tct caa aaa cgc gtt gta gta ttg ggt tcg gga gtc ata ggt       48
Met His Ser Gln Lys Arg Val Val Val Leu Gly Ser Gly Val Ile Gly         
1               5                   10                  15              

ctt tct tct gct ctt ata ctt gca cgc aag ggc tat tct gtt cat att       96
Leu Ser Ser Ala Leu Ile Leu Ala Arg Lys Gly Tyr Ser Val His Ile         
            20                  25                  30                  

ttg gca cgc gat ctc cca gag gac gta tct agc caa act ttc gct tcc      144
Leu Ala Arg Asp Leu Pro Glu Asp Val Ser Ser Gln Thr Phe Ala Ser         
        35                  40                  45                      

ccc tgg gct ggt gcg aat tgg aca cca ttt atg aca ttg act gac ggc      192
Pro Trp Ala Gly Ala Asn Trp Thr Pro Phe Met Thr Leu Thr Asp Gly         
    50                  55                  60                          

cct aga cag gca aaa tgg gaa gag agt acc ttt aaa aaa tgg gtt gag      240
Pro Arg Gln Ala Lys Trp Glu Glu Ser Thr Phe Lys Lys Trp Val Glu         
65                  70                  75                  80          

ttg gtc cct acc gga cat gct atg tgg ctg aag gga act cgt cgc ttt      288
Leu Val Pro Thr Gly His Ala Met Trp Leu Lys Gly Thr Arg Arg Phe         
                85                  90                  95              

gca cag aat gaa gac ggc ttg ctt gga cat tgg tac aaa gat ata acc      336
Ala Gln Asn Glu Asp Gly Leu Leu Gly His Trp Tyr Lys Asp Ile Thr         
            100                 105                 110                 

ccg aat tac cgc ccc ctt ccc tcg tct gaa tgt cca cca ggc gca att      384
Pro Asn Tyr Arg Pro Leu Pro Ser Ser Glu Cys Pro Pro Gly Ala Ile         
        115                 120                 125                     

ggc gtg act tat gac aca ctc tct gta cac gct cct aaa tat tgt cag      432
Gly Val Thr Tyr Asp Thr Leu Ser Val His Ala Pro Lys Tyr Cys Gln         
    130                 135                 140                         

tat ctt gcc aga gaa ctt cag aaa ctc ggt gca act ttt gaa aga cgg      480
Tyr Leu Ala Arg Glu Leu Gln Lys Leu Gly Ala Thr Phe Glu Arg Arg         
145                 150                 155                 160         

acg gtt acc tca ctg gaa cag gca ttc gac ggt gca gac ctc gtg gtg      528
Thr Val Thr Ser Leu Glu Gln Ala Phe Asp Gly Ala Asp Leu Val Val         
                165                 170                 175             

aat gca aca gga ctg gga gca aag agc ata gca ggc ata gat gat caa      576
Asn Ala Thr Gly Leu Gly Ala Lys Ser Ile Ala Gly Ile Asp Asp Gln         
            180                 185                 190                 

gct gcg gag cca att aga ggc cag acc gtg ctg gta aaa tca cca tgc      624
Ala Ala Glu Pro Ile Arg Gly Gln Thr Val Leu Val Lys Ser Pro Cys         
        195                 200                 205                     

aaa aga tgt acg atg gat agt agt gac cca gcg agt cca gct tat att      672
Lys Arg Cys Thr Met Asp Ser Ser Asp Pro Ala Ser Pro Ala Tyr Ile         
    210                 215                 220                         

ata ccg aga ccg ggt ggc gaa gtt ata tgc gga gga act tac gga gtg      720
Ile Pro Arg Pro Gly Gly Glu Val Ile Cys Gly Gly Thr Tyr Gly Val         
225                 230                 235                 240         

ggt gat tgg gat ctt agc gtt aac ccc gaa aca gtc caa cgg att ctg      768
Gly Asp Trp Asp Leu Ser Val Asn Pro Glu Thr Val Gln Arg Ile Leu         
                245                 250                 255             

aag cat tgc ctc cgc ctt gac cca acc ata tca tct gac gga acc att      816
Lys His Cys Leu Arg Leu Asp Pro Thr Ile Ser Ser Asp Gly Thr Ile         
            260                 265                 270                 

gag gga atc gaa gta ctt agg cat aat gtt ggt ctt agg ccc gct cgt      864
Glu Gly Ile Glu Val Leu Arg His Asn Val Gly Leu Arg Pro Ala Arg         
        275                 280                 285                     

aga gga gga cca cgt gta gaa gct gag aga att gta ttg ccc ctc gat      912
Arg Gly Gly Pro Arg Val Glu Ala Glu Arg Ile Val Leu Pro Leu Asp         
    290                 295                 300                         

aga acc aaa agc ccc ctt tcg ctt gga aga gga agc gca cgt gca gct      960
Arg Thr Lys Ser Pro Leu Ser Leu Gly Arg Gly Ser Ala Arg Ala Ala         
305                 310                 315                 320         

aag gaa aaa gaa gtc aca ctg gta cat gca tat ggt ttt agt agt gcc     1008
Lys Glu Lys Glu Val Thr Leu Val His Ala Tyr Gly Phe Ser Ser Ala         
                325                 330                 335             

gga tat cag caa tca tgg ggt gct gca gaa gat gtt gct cag ttg gtg     1056
Gly Tyr Gln Gln Ser Trp Gly Ala Ala Glu Asp Val Ala Gln Leu Val         
            340                 345                 350                 

gac gag gca ttc caa aga tac cac ggt gca gcg aga gag tca aaa ctt     1104
Asp Glu Ala Phe Gln Arg Tyr His Gly Ala Ala Arg Glu Ser Lys Leu         
        355                 360                 365                     

tag                                                                 1107


<210>  16
<211>  368
<212>  PRT
<213>  Rhodosporidium toruloides

<400>  16

Met His Ser Gln Lys Arg Val Val Val Leu Gly Ser Gly Val Ile Gly 
1               5                   10                  15      


Leu Ser Ser Ala Leu Ile Leu Ala Arg Lys Gly Tyr Ser Val His Ile 
            20                  25                  30          


Leu Ala Arg Asp Leu Pro Glu Asp Val Ser Ser Gln Thr Phe Ala Ser 
        35                  40                  45              


Pro Trp Ala Gly Ala Asn Trp Thr Pro Phe Met Thr Leu Thr Asp Gly 
    50                  55                  60                  


Pro Arg Gln Ala Lys Trp Glu Glu Ser Thr Phe Lys Lys Trp Val Glu 
65                  70                  75                  80  


Leu Val Pro Thr Gly His Ala Met Trp Leu Lys Gly Thr Arg Arg Phe 
                85                  90                  95      


Ala Gln Asn Glu Asp Gly Leu Leu Gly His Trp Tyr Lys Asp Ile Thr 
            100                 105                 110         


Pro Asn Tyr Arg Pro Leu Pro Ser Ser Glu Cys Pro Pro Gly Ala Ile 
        115                 120                 125             


Gly Val Thr Tyr Asp Thr Leu Ser Val His Ala Pro Lys Tyr Cys Gln 
    130                 135                 140                 


Tyr Leu Ala Arg Glu Leu Gln Lys Leu Gly Ala Thr Phe Glu Arg Arg 
145                 150                 155                 160 


Thr Val Thr Ser Leu Glu Gln Ala Phe Asp Gly Ala Asp Leu Val Val 
                165                 170                 175     


Asn Ala Thr Gly Leu Gly Ala Lys Ser Ile Ala Gly Ile Asp Asp Gln 
            180                 185                 190         


Ala Ala Glu Pro Ile Arg Gly Gln Thr Val Leu Val Lys Ser Pro Cys 
        195                 200                 205             


Lys Arg Cys Thr Met Asp Ser Ser Asp Pro Ala Ser Pro Ala Tyr Ile 
    210                 215                 220                 


Ile Pro Arg Pro Gly Gly Glu Val Ile Cys Gly Gly Thr Tyr Gly Val 
225                 230                 235                 240 


Gly Asp Trp Asp Leu Ser Val Asn Pro Glu Thr Val Gln Arg Ile Leu 
                245                 250                 255     


Lys His Cys Leu Arg Leu Asp Pro Thr Ile Ser Ser Asp Gly Thr Ile 
            260                 265                 270         


Glu Gly Ile Glu Val Leu Arg His Asn Val Gly Leu Arg Pro Ala Arg 
        275                 280                 285             


Arg Gly Gly Pro Arg Val Glu Ala Glu Arg Ile Val Leu Pro Leu Asp 
    290                 295                 300                 


Arg Thr Lys Ser Pro Leu Ser Leu Gly Arg Gly Ser Ala Arg Ala Ala 
305                 310                 315                 320 


Lys Glu Lys Glu Val Thr Leu Val His Ala Tyr Gly Phe Ser Ser Ala 
                325                 330                 335     


Gly Tyr Gln Gln Ser Trp Gly Ala Ala Glu Asp Val Ala Gln Leu Val 
            340                 345                 350         


Asp Glu Ala Phe Gln Arg Tyr His Gly Ala Ala Arg Glu Ser Lys Leu 
        355                 360                 365             


<210>  17
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  Nucleic acid sequence encoding qPCR primer GUSCommon-341F

<400>  17
ccgggtgaag gttatctcta tga                                             23


<210>  18
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  Nucleic acid sequence encoding qPCR primer GUSCommon-414R

<400>  18
cgaagcgggt agatatcaca ctct                                            24


<210>  19
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  Nucleic acid sequence encoding qPCR probe GUSCommon-366FAM

<400>  19
tgtgcgtcac agccaaaagc caga                                            24


<210>  20
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Nucleic acid sequence encoding qPCR primer EcdsdA-860F

<400>  20
tcgcattcgg gcttaaactg                                                 20


<210>  21
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  Nucleic acid sequence encoding qPCR primer EcdsdA-922R

<400>  21
gcgttggttc ggcaaaaa                                                   18


<210>  22
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Nucleic acid sequence encoding qPCR probe EcdsdA-883FAM

<400>  22
tttggcgatc atgttcactg c                                               21


<210>  23
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  Nucleic acid sequence encoding qPCR primer TaGBSS:1-F

<400>  23
ttctgcatcc acaacatctc gta                                             23


<210>  24
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Nucleic acid sequence encoding qPCR primer TaGBSS:1-R

<400>  24
tagccgtcga tgaagtcgaa                                                 20


<210>  25
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  Nucleic acid sequence encoding qPCR probe TaGBSS:1-TET

<400>  25
cgacgacttc gcgcagctca ac                                              22


<210>  26
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  Nucleic acid sequence encoding qPCR primer dao1/pa-285F

<400>  26
gttcgcgcag aacgaagac                                                  19


<210>  27
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  Nucleic acid sequence encoding qPCR primer dao1/pa-349R

<400>  27
ggcggtaatt tggcgtga                                                   18


<210>  28
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  Nucleic acid sequence encoding qPCR probe dao1/pa-308FAM

<400>  28
tccttgtacc agtgcccgag ca                                              22


<210>  29
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Nucleic acid sequence encoding forward PCR primer for gusINT gene

<400>  29
accgtttgtg tgaacaacga                                                 20


<210>  30
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Nucleic acid sequence encoding reverse PCR primer for gusINT gene

<400>  30
ggcacagcac atcaaagaga                                                 20


<210>  31
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  Nucleic acid sequence encoding forward PCR primer for dsdA gene

<400>  31
gctttttgtt cgcttggttg tg                                              22


<210>  32
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Nucleic acid sequence encoding reverse PCR primer for dsdA gene

<400>  32
tcaataatcc ccccagtggc                                                 20


<210>  33
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  Nucleic acid sequence encoding forward PCR primer for dao1 gene

<400>  33
gacaagcaaa atgggaagaa tc                                              22


<210>  34
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  Nucleic acid sequence encoding reverse PCR primer for dao1 gene

<400>  34
tcggggaatg atgtaggc                                                   18


<210>  35
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  Nucleic acid sequence encoding forward PCR primer for dao1/ko 
       gene

<400>  35
aagcaggcct tctcacactt ga                                              22


<210>  36
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Nucleic acid sequence encoding reverse PCR primer for dao1/ko 
       gene

<400>  36
ttccaacaaa gcccgatgcg                                                 20


<210>  37
<211>  29
<212>  DNA
<213>  Artificial

<220>
<223>  Nucleic acid sequence encoding forward PCR primer for PAT gene

<400>  37
atgtctccgg agaggagacc agttgagat                                       29


<210>  38
<211>  26
<212>  DNA
<213>  Artificial

<220>
<223>  Nucleic acid sequence encoding reverse PCR primer for PAT gene

<400>  38
gccaaaaacc aacatcatgc catcca                                          26


<210>  39
<211>  4503
<212>  DNA
<213>  Artificial

<220>
<223>  T-DNA region from RLM151, a RLM175-type binary vector containing 
       a p-ZmUBI+I::c-dsdA::t-OCS selection cassette.


<220>
<221>  misc_structure
<222>  (1)..(128)
<223>  Left T-DNA border from pSB11

<220>
<221>  misc_binding
<222>  (47)..(71)
<223>  Left T-DNA border repeat from pSB11

<220>
<221>  promoter
<222>  (162)..(2149)
<223>  Zea mays ubiquitin promoter region including intron

<220>
<221>  Intron
<222>  (1144)..(2149)
<223>  Zea mays ubiquitin intron MubG1

<220>
<221>  CDS
<222>  (2192)..(3520)
<223>  E. coli D-serine deaminase [dsdA] CDS

<220>
<221>  terminator
<222>  (3562)..(4270)
<223>  Agrobacterium tumefaciens octapine synthase terminator region

<220>
<221>  misc_structure
<222>  (4359)..(4503)
<223>  Right T-DNA border from pSB11

<220>
<221>  misc_binding
<222>  (4399)..(4422)
<223>  Right T-DNA border repeat from pSB11

<400>  39
gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt     60

gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt    120

actgaattgg atccgcccgg gcggtaccaa gcttccgcgg ctgcagtgca gcgtgacccg    180

gtcgtgcccc tctctagaga taatgagcat tgcatgtcta agttataaaa aattaccaca    240

tatttttttt gtcacacttg tttgaagtgc agtttatcta tctttataca tatatttaaa    300

ctttactcta cgaataatat aatctatagt actacaataa tatcagtgtt ttagagaatc    360

atataaatga acagttagac atggtctaaa ggacaattga gtattttgac aacaggactc    420

tacagtttta tctttttagt gtgcatgtgt tctccttttt ttttgcaaat agcttcacct    480

atataatact tcatccattt tattagtaca tccatttagg gtttagggtt aatggttttt    540

atagactaat ttttttagta catctatttt attctatttt agcctctaaa ttaagaaaac    600

taaaactcta ttttagtttt tttatttaat agtttagata taaaatagaa taaaataaag    660

tgactaaaaa ttaaacaaat accctttaag aaattaaaaa aactaaggaa acatttttct    720

tgtttcgagt agataatgcc agcctgttaa acgccgtcga cgagtctaac ggacaccaac    780

cagcgaacca gcagcgtcgc gtcgggccaa gcgaagcaga cggcacggca tctctgtcgc    840

tgcctctgga cccctctcga gagttccgct ccaccgttgg acttgctccg ctgtcggcat    900

ccagaaattg cgtggcggag cggcagacgt gagccggcac ggcaggcggc ctcctcctcc    960

tctcacggca ccggcagcta cgggggattc ctttcccacc gctccttcgc tttcccttcc   1020

tcgcccgccg taataaatag acaccccctc cacaccctct ttccccaacc tcgtgttgtt   1080

cggagcgcac acacacacaa ccagatctcc cccaaatcca cccgtcggca cctccgcttc   1140

aaggtacgcc gctcgtcctc cccccccccc cccctctcta ccttctctag atcggcgttc   1200

cggtccatgg ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt   1260

tgtgttagat ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt   1320

ctgattgcta acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc   1380

gcagacggga tcgatttcat gatttttttt gtttcgttgc atagggtttg gtttgccctt   1440

ttcctttatt tcaatatatg ccgtgcactt gtttgtcggg tcatcttttc atgctttttt   1500

ttgtcttggt tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtagaattc   1560

tgtttcaaac tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat   1620

tcatagttac gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt   1680

gatgcgggtt ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg   1740

tggtgtggtt gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta   1800

cctggtgtat ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga   1860

gtttaagatg gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact   1920

gatgcatata catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta   1980

tctattataa taaacaagta tgttttataa ttatttcgat cttgatatac ttggatgatg   2040

gcatatgcag cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc   2100

ttggtactgt ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcagggtac   2160

ggatcctcat ctaagcgcaa agagacgtac t atg gaa aac gct aaa atg aac      2212
                                   Met Glu Asn Ala Lys Met Asn          
                                   1               5                    

tcg ctc atc gcc cag tat ccg ttg gta aag gat ctg gtt gct ctt aaa     2260
Ser Leu Ile Ala Gln Tyr Pro Leu Val Lys Asp Leu Val Ala Leu Lys         
        10                  15                  20                      

gaa acc acc tgg ttt aat cct ggc acg acc tca ttg gct gaa ggt tta     2308
Glu Thr Thr Trp Phe Asn Pro Gly Thr Thr Ser Leu Ala Glu Gly Leu         
    25                  30                  35                          

cct tat gtt ggc ctg acc gaa cag gat gtt cag gac gcc cat gcg cgc     2356
Pro Tyr Val Gly Leu Thr Glu Gln Asp Val Gln Asp Ala His Ala Arg         
40                  45                  50                  55          

tta tcc cgt ttt gca ccc tat ctg gca aaa gca ttt cct gaa act gct     2404
Leu Ser Arg Phe Ala Pro Tyr Leu Ala Lys Ala Phe Pro Glu Thr Ala         
                60                  65                  70              

gcc act ggg ggg att att gaa tca gaa ctg gtt gcc att cca gct atg     2452
Ala Thr Gly Gly Ile Ile Glu Ser Glu Leu Val Ala Ile Pro Ala Met         
            75                  80                  85                  

caa aaa cgg ctg gaa aaa gaa tat cag caa ccg atc agc ggg caa ctg     2500
Gln Lys Arg Leu Glu Lys Glu Tyr Gln Gln Pro Ile Ser Gly Gln Leu         
        90                  95                  100                     

tta ctg aaa aaa gat agc cat ttg ccc att tcc ggc tcc ata aaa gca     2548
Leu Leu Lys Lys Asp Ser His Leu Pro Ile Ser Gly Ser Ile Lys Ala         
    105                 110                 115                         

cgc ggc ggg att tat gaa gtc ctg gca cac gca gaa aaa ctg gct ctg     2596
Arg Gly Gly Ile Tyr Glu Val Leu Ala His Ala Glu Lys Leu Ala Leu         
120                 125                 130                 135         

gaa gcg ggg ttg ctg acg ctt gat gat gac tac agc aaa ctg ctt tct     2644
Glu Ala Gly Leu Leu Thr Leu Asp Asp Asp Tyr Ser Lys Leu Leu Ser         
                140                 145                 150             

ccg gag ttt aaa cag ttc ttt agc caa tac agc att gct gtg ggc tca     2692
Pro Glu Phe Lys Gln Phe Phe Ser Gln Tyr Ser Ile Ala Val Gly Ser         
            155                 160                 165                 

acc gga aat ctg ggg tta tca atc ggc att atg agc gcc cgc att ggc     2740
Thr Gly Asn Leu Gly Leu Ser Ile Gly Ile Met Ser Ala Arg Ile Gly         
        170                 175                 180                     

ttt aag gtg aca gtt cat atg tct gct gat gcc cgg gca tgg aaa aaa     2788
Phe Lys Val Thr Val His Met Ser Ala Asp Ala Arg Ala Trp Lys Lys         
    185                 190                 195                         

gcg aaa ctg cgc agc cat ggc gtt acg gtc gtg gaa tat gag caa gat     2836
Ala Lys Leu Arg Ser His Gly Val Thr Val Val Glu Tyr Glu Gln Asp         
200                 205                 210                 215         

tat ggt gtt gcc gtc gag gaa gga cgt aaa gca gcg cag tct gac ccg     2884
Tyr Gly Val Ala Val Glu Glu Gly Arg Lys Ala Ala Gln Ser Asp Pro         
                220                 225                 230             

aac tgt ttc ttt att gat gac gaa aat tcc cgc acg ttg ttc ctt ggg     2932
Asn Cys Phe Phe Ile Asp Asp Glu Asn Ser Arg Thr Leu Phe Leu Gly         
            235                 240                 245                 

tat tcc gtc gct ggc cag cgt ctt aaa gcg caa ttt gcc cag caa ggc     2980
Tyr Ser Val Ala Gly Gln Arg Leu Lys Ala Gln Phe Ala Gln Gln Gly         
        250                 255                 260                     

cgt atc gtc gat gct gat aac cct ctg ttt gtc tat ctg ccg tgt ggt     3028
Arg Ile Val Asp Ala Asp Asn Pro Leu Phe Val Tyr Leu Pro Cys Gly         
    265                 270                 275                         

gtt ggc ggt ggt cct ggt ggc gtc gca ttc ggg ctt aaa ctg gcg ttt     3076
Val Gly Gly Gly Pro Gly Gly Val Ala Phe Gly Leu Lys Leu Ala Phe         
280                 285                 290                 295         

ggc gat cat gtt cac tgc ttt ttt gcc gaa cca acg cac tcc cct tgt     3124
Gly Asp His Val His Cys Phe Phe Ala Glu Pro Thr His Ser Pro Cys         
                300                 305                 310             

atg ttg tta ggc gtc cat aca gga tta cac gat cag att tct gtt cag     3172
Met Leu Leu Gly Val His Thr Gly Leu His Asp Gln Ile Ser Val Gln         
            315                 320                 325                 

gat att ggt atc gac aac ctt acc gca gcg gat ggc ctt gca gtt ggt     3220
Asp Ile Gly Ile Asp Asn Leu Thr Ala Ala Asp Gly Leu Ala Val Gly         
        330                 335                 340                     

cgc gca tca ggc ttt gtc ggg cgg gca atg gag cgt ctg ctg gat ggc     3268
Arg Ala Ser Gly Phe Val Gly Arg Ala Met Glu Arg Leu Leu Asp Gly         
    345                 350                 355                         

ttc tat acc ctt agc gat caa acc atg tat gac atg ctt ggc tgg ctg     3316
Phe Tyr Thr Leu Ser Asp Gln Thr Met Tyr Asp Met Leu Gly Trp Leu         
360                 365                 370                 375         

gcg cag gaa gaa ggt att cgt ctt gaa cct tcg gca ctg gcg ggt atg     3364
Ala Gln Glu Glu Gly Ile Arg Leu Glu Pro Ser Ala Leu Ala Gly Met         
                380                 385                 390             

gcc gga cct cag cgc gtg tgt gca tca gta agt tac caa cag atg cac     3412
Ala Gly Pro Gln Arg Val Cys Ala Ser Val Ser Tyr Gln Gln Met His         
            395                 400                 405                 

ggt ttc agc gca gaa caa ctg cgt aat acc act cat ctg gtg tgg gcg     3460
Gly Phe Ser Ala Glu Gln Leu Arg Asn Thr Thr His Leu Val Trp Ala         
        410                 415                 420                     

acg gga ggt gga atg gtg ccg gaa gaa gag atg aat caa tat ctg gca     3508
Thr Gly Gly Gly Met Val Pro Glu Glu Glu Met Asn Gln Tyr Leu Ala         
    425                 430                 435                         

aaa ggc cgt taa taacgtttca acgcagcatg gatcgtaccg agctcaatcg         3560
Lys Gly Arg                                                             
440                                                                     

atcctgcttt aatgagatat gcgagacgcc tatgatcgca tgatatttgc tttcaattct   3620

gttgtgcacg ttgtaaaaaa cctgagcatg tgtagctcag atccttaccg ccggtttcgg   3680

ttcattctaa tgaatatatc acccgttact atcgtatttt tatgaataat attctccgtt   3740

caatttactg attgtaccct actacttata tgtacaatat taaaatgaaa acaatatatt   3800

gtgctgaata ggtttatagc gacatctatg atagagcgcc acaataacaa acaattgcgt   3860

tttattatta caaatccaat tttaaaaaaa gcggcagaac cggtcaaacc taaaagactg   3920

attacataaa tcttattcaa atttcaaaag tgccccaggg gctagtatct acgacacacc   3980

gagcggcgaa ctaataacgc tcactgaagg gaactccggt tccccgccgg cgcgcatggg   4040

tgagattcct tgaagttgag tattggccgt ccgctctacc gaaagttacg ggcaccattc   4100

aacccggtcc agcacggcgg ccgggtaacc gacttgctgc cccgagaatt atgcagcatt   4160

tttttggtgt atgtgggccc caaatgaagt gcaggtcaaa ccttgacagt gacgacaaat   4220

cgttgggcgg gtccagggcg aattttgcga caacatgtcg aggctcagca ggatgggccc   4280

aggtacagaa ttcgcggccg tacaacgcgt accggttaat taatctagag gcgcgccggg   4340

cccggccggc cagatcttga ttgtcgtttc ccgccttcag tttaaactat cagtgtttga   4400

caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata atcggatatt   4460

taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgt                     4503


<210>  40
<211>  442
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Construct E. coli D-serine deaminase [dsdA] CDS

<400>  40

Met Glu Asn Ala Lys Met Asn Ser Leu Ile Ala Gln Tyr Pro Leu Val 
1               5                   10                  15      


Lys Asp Leu Val Ala Leu Lys Glu Thr Thr Trp Phe Asn Pro Gly Thr 
            20                  25                  30          


Thr Ser Leu Ala Glu Gly Leu Pro Tyr Val Gly Leu Thr Glu Gln Asp 
        35                  40                  45              


Val Gln Asp Ala His Ala Arg Leu Ser Arg Phe Ala Pro Tyr Leu Ala 
    50                  55                  60                  


Lys Ala Phe Pro Glu Thr Ala Ala Thr Gly Gly Ile Ile Glu Ser Glu 
65                  70                  75                  80  


Leu Val Ala Ile Pro Ala Met Gln Lys Arg Leu Glu Lys Glu Tyr Gln 
                85                  90                  95      


Gln Pro Ile Ser Gly Gln Leu Leu Leu Lys Lys Asp Ser His Leu Pro 
            100                 105                 110         


Ile Ser Gly Ser Ile Lys Ala Arg Gly Gly Ile Tyr Glu Val Leu Ala 
        115                 120                 125             


His Ala Glu Lys Leu Ala Leu Glu Ala Gly Leu Leu Thr Leu Asp Asp 
    130                 135                 140                 


Asp Tyr Ser Lys Leu Leu Ser Pro Glu Phe Lys Gln Phe Phe Ser Gln 
145                 150                 155                 160 


Tyr Ser Ile Ala Val Gly Ser Thr Gly Asn Leu Gly Leu Ser Ile Gly 
                165                 170                 175     


Ile Met Ser Ala Arg Ile Gly Phe Lys Val Thr Val His Met Ser Ala 
            180                 185                 190         


Asp Ala Arg Ala Trp Lys Lys Ala Lys Leu Arg Ser His Gly Val Thr 
        195                 200                 205             


Val Val Glu Tyr Glu Gln Asp Tyr Gly Val Ala Val Glu Glu Gly Arg 
    210                 215                 220                 


Lys Ala Ala Gln Ser Asp Pro Asn Cys Phe Phe Ile Asp Asp Glu Asn 
225                 230                 235                 240 


Ser Arg Thr Leu Phe Leu Gly Tyr Ser Val Ala Gly Gln Arg Leu Lys 
                245                 250                 255     


Ala Gln Phe Ala Gln Gln Gly Arg Ile Val Asp Ala Asp Asn Pro Leu 
            260                 265                 270         


Phe Val Tyr Leu Pro Cys Gly Val Gly Gly Gly Pro Gly Gly Val Ala 
        275                 280                 285             


Phe Gly Leu Lys Leu Ala Phe Gly Asp His Val His Cys Phe Phe Ala 
    290                 295                 300                 


Glu Pro Thr His Ser Pro Cys Met Leu Leu Gly Val His Thr Gly Leu 
305                 310                 315                 320 


His Asp Gln Ile Ser Val Gln Asp Ile Gly Ile Asp Asn Leu Thr Ala 
                325                 330                 335     


Ala Asp Gly Leu Ala Val Gly Arg Ala Ser Gly Phe Val Gly Arg Ala 
            340                 345                 350         


Met Glu Arg Leu Leu Asp Gly Phe Tyr Thr Leu Ser Asp Gln Thr Met 
        355                 360                 365             


Tyr Asp Met Leu Gly Trp Leu Ala Gln Glu Glu Gly Ile Arg Leu Glu 
    370                 375                 380                 


Pro Ser Ala Leu Ala Gly Met Ala Gly Pro Gln Arg Val Cys Ala Ser 
385                 390                 395                 400 


Val Ser Tyr Gln Gln Met His Gly Phe Ser Ala Glu Gln Leu Arg Asn 
                405                 410                 415     


Thr Thr His Leu Val Trp Ala Thr Gly Gly Gly Met Val Pro Glu Glu 
            420                 425                 430         


Glu Met Asn Gln Tyr Leu Ala Lys Gly Arg 
        435                 440         


<210>  41
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Forward PCR primer for ahas gene

<400>  41
tgactttggc tcarggaacg                                                 20


<210>  42
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  Reverse PCR primer for ahas gene

<400>  42
atctcacttt cattctctgg gttt                                            24


