                         SEQUENCE LISTING



<110>  BASF Plant Science GmbH



<120>  Verfahren zur Herstellung von Arachidonsure und/oder Eicosapentaensure in transgenen Nutzpflanzen



<130>  PF56991



<140>  20050640

<141>  2005-08-08



<160>  12    



<170>  PatentIn version 3.1



<210>  1

<211>  1380

<212>  DNA

<213>  Pythium irregulare



<220>

<221>  CDS

<222>  (1)..(1380)

<223>  Delta-6-Desaturase



<400>  1
atg gtg gac ctc aag cct gga gtg aag cgc ctg gtg agc tgg aag gag       48
Met Val Asp Leu Lys Pro Gly Val Lys Arg Leu Val Ser Trp Lys Glu         
1               5                   10                  15              

atc cgc gag cac gcg acg ccc gcg acc gcg tgg atc gtg att cac cac       96
Ile Arg Glu His Ala Thr Pro Ala Thr Ala Trp Ile Val Ile His His         
            20                  25                  30                  

aag gtc tac gac atc tcc aag tgg gac tcg cac ccg ggt ggc tcc gtg      144
Lys Val Tyr Asp Ile Ser Lys Trp Asp Ser His Pro Gly Gly Ser Val         
        35                  40                  45                      

atg ctc acg cag gcc ggc gag gac gcc acg gac gcc ttc gcg gtc ttc      192
Met Leu Thr Gln Ala Gly Glu Asp Ala Thr Asp Ala Phe Ala Val Phe         
    50                  55                  60                          

cac ccg tcc tcg gcg ctc aag ctg ctc gag cag ttc tac gtc ggc gac      240
His Pro Ser Ser Ala Leu Lys Leu Leu Glu Gln Phe Tyr Val Gly Asp         
65                  70                  75                  80          

gtg gac gaa acc tcc aag gcc gag atc gag ggg gag ccg gcg agc gac      288
Val Asp Glu Thr Ser Lys Ala Glu Ile Glu Gly Glu Pro Ala Ser Asp         
                85                  90                  95              

gag gag cgc gcg cgc cgc gag cgc atc aac gag ttc atc gcg tcc tac      336
Glu Glu Arg Ala Arg Arg Glu Arg Ile Asn Glu Phe Ile Ala Ser Tyr         
            100                 105                 110                 

cgc cgt ctg cgc gtc aag gtc aag ggc atg ggg ctc tac gac gcc agc      384
Arg Arg Leu Arg Val Lys Val Lys Gly Met Gly Leu Tyr Asp Ala Ser         
        115                 120                 125                     

gcg ctc tac tac gcg tgg aag ctc gtg agc acg ttc ggc atc gcg gtg      432
Ala Leu Tyr Tyr Ala Trp Lys Leu Val Ser Thr Phe Gly Ile Ala Val         
    130                 135                 140                         

ctc tcg atg gcg atc tgc ttc ttc ttc aac agt ttc gcc atg tac atg      480
Leu Ser Met Ala Ile Cys Phe Phe Phe Asn Ser Phe Ala Met Tyr Met         
145                 150                 155                 160         

gtc gcc ggc gtg att atg ggg ctc ttc tac cag cag tcc gga tgg ctg      528
Val Ala Gly Val Ile Met Gly Leu Phe Tyr Gln Gln Ser Gly Trp Leu         
                165                 170                 175             

gcg cac gac ttc ttg cac aac cag gtg tgc gag aac cgc acg ctc ggc      576
Ala His Asp Phe Leu His Asn Gln Val Cys Glu Asn Arg Thr Leu Gly         
            180                 185                 190                 

aac ctt atc ggc tgc ctc gtg ggc aac gcc tgg cag ggc ttc agc atg      624
Asn Leu Ile Gly Cys Leu Val Gly Asn Ala Trp Gln Gly Phe Ser Met         
        195                 200                 205                     

cag tgg tgg aag aac aag cac aac ctg cac cac gcg gtg ccg aac ctg      672
Gln Trp Trp Lys Asn Lys His Asn Leu His His Ala Val Pro Asn Leu         
    210                 215                 220                         

cac agc gcc aag gac gag ggc ttc atc ggc gac ccg gac atc gac acc      720
His Ser Ala Lys Asp Glu Gly Phe Ile Gly Asp Pro Asp Ile Asp Thr         
225                 230                 235                 240         

atg ccg ctg ctg gcg tgg tct aag gag atg gcg cgc aag gcg ttc gag      768
Met Pro Leu Leu Ala Trp Ser Lys Glu Met Ala Arg Lys Ala Phe Glu         
                245                 250                 255             

tcg gcg cac ggc ccg ttc ttc atc cgc aac cag gcg ttc cta tac ttc      816
Ser Ala His Gly Pro Phe Phe Ile Arg Asn Gln Ala Phe Leu Tyr Phe         
            260                 265                 270                 

ccg ctg ctg ctg ctc gcg cgc ctg agc tgg ctc gcg cag tcg ttc ttc      864
Pro Leu Leu Leu Leu Ala Arg Leu Ser Trp Leu Ala Gln Ser Phe Phe         
        275                 280                 285                     

tac gtg ttc acc gag ttc tcg ttc ggc atc ttc gac aag gtc gag ttc      912
Tyr Val Phe Thr Glu Phe Ser Phe Gly Ile Phe Asp Lys Val Glu Phe         
    290                 295                 300                         

gac gga ccg gag aag gcg ggt ctg atc gtg cac tac atc tgg cag ctc      960
Asp Gly Pro Glu Lys Ala Gly Leu Ile Val His Tyr Ile Trp Gln Leu         
305                 310                 315                 320         

gcg atc ccg tac ttc tgc aac atg agc ctg ttt gag ggc gtg gca tac     1008
Ala Ile Pro Tyr Phe Cys Asn Met Ser Leu Phe Glu Gly Val Ala Tyr         
                325                 330                 335             

ttc ctc atg ggc cag gcg tcc tgc ggc ttg ctc ctg gcg ctg gtg ttc     1056
Phe Leu Met Gly Gln Ala Ser Cys Gly Leu Leu Leu Ala Leu Val Phe         
            340                 345                 350                 

agt att ggc cac aac ggc atg tcg gtg tac gag cgc gaa acc aag ccg     1104
Ser Ile Gly His Asn Gly Met Ser Val Tyr Glu Arg Glu Thr Lys Pro         
        355                 360                 365                     

gac ttc tgg cag ctg cag gtg acc acg acg cgc aac atc cgc gcg tcg     1152
Asp Phe Trp Gln Leu Gln Val Thr Thr Thr Arg Asn Ile Arg Ala Ser         
    370                 375                 380                         

gta ttc atg gac tgg ttc acc ggt ggc ttg aac tac cag atc gac cat     1200
Val Phe Met Asp Trp Phe Thr Gly Gly Leu Asn Tyr Gln Ile Asp His         
385                 390                 395                 400         

cac ctg ttc ccg ctc gtg ccg cgc cac aac ttg cca aag gtc aac gtg     1248
His Leu Phe Pro Leu Val Pro Arg His Asn Leu Pro Lys Val Asn Val         
                405                 410                 415             

ctc atc aag tcg cta tgc aag gag ttc gac atc ccg ttc cac gag acc     1296
Leu Ile Lys Ser Leu Cys Lys Glu Phe Asp Ile Pro Phe His Glu Thr         
            420                 425                 430                 

ggc ttc tgg gag ggc atc tac gag gtc gtg gac cac ctg gcg gac atc     1344
Gly Phe Trp Glu Gly Ile Tyr Glu Val Val Asp His Leu Ala Asp Ile         
        435                 440                 445                     

agc aag gaa ttt atc acc gag ttc cca gcg atg taa                     1380
Ser Lys Glu Phe Ile Thr Glu Phe Pro Ala Met                             
    450                 455                                             


<210>  2

<211>  459

<212>  PRT

<213>  Pythium irregulare



<400>  2

Met Val Asp Leu Lys Pro Gly Val Lys Arg Leu Val Ser Trp Lys Glu 
1               5                   10                  15      


Ile Arg Glu His Ala Thr Pro Ala Thr Ala Trp Ile Val Ile His His 
            20                  25                  30          


Lys Val Tyr Asp Ile Ser Lys Trp Asp Ser His Pro Gly Gly Ser Val 
        35                  40                  45              


Met Leu Thr Gln Ala Gly Glu Asp Ala Thr Asp Ala Phe Ala Val Phe 
    50                  55                  60                  


His Pro Ser Ser Ala Leu Lys Leu Leu Glu Gln Phe Tyr Val Gly Asp 
65                  70                  75                  80  


Val Asp Glu Thr Ser Lys Ala Glu Ile Glu Gly Glu Pro Ala Ser Asp 
                85                  90                  95      


Glu Glu Arg Ala Arg Arg Glu Arg Ile Asn Glu Phe Ile Ala Ser Tyr 
            100                 105                 110         


Arg Arg Leu Arg Val Lys Val Lys Gly Met Gly Leu Tyr Asp Ala Ser 
        115                 120                 125             


Ala Leu Tyr Tyr Ala Trp Lys Leu Val Ser Thr Phe Gly Ile Ala Val 
    130                 135                 140                 


Leu Ser Met Ala Ile Cys Phe Phe Phe Asn Ser Phe Ala Met Tyr Met 
145                 150                 155                 160 


Val Ala Gly Val Ile Met Gly Leu Phe Tyr Gln Gln Ser Gly Trp Leu 
                165                 170                 175     


Ala His Asp Phe Leu His Asn Gln Val Cys Glu Asn Arg Thr Leu Gly 
            180                 185                 190         


Asn Leu Ile Gly Cys Leu Val Gly Asn Ala Trp Gln Gly Phe Ser Met 
        195                 200                 205             


Gln Trp Trp Lys Asn Lys His Asn Leu His His Ala Val Pro Asn Leu 
    210                 215                 220                 


His Ser Ala Lys Asp Glu Gly Phe Ile Gly Asp Pro Asp Ile Asp Thr 
225                 230                 235                 240 


Met Pro Leu Leu Ala Trp Ser Lys Glu Met Ala Arg Lys Ala Phe Glu 
                245                 250                 255     


Ser Ala His Gly Pro Phe Phe Ile Arg Asn Gln Ala Phe Leu Tyr Phe 
            260                 265                 270         


Pro Leu Leu Leu Leu Ala Arg Leu Ser Trp Leu Ala Gln Ser Phe Phe 
        275                 280                 285             


Tyr Val Phe Thr Glu Phe Ser Phe Gly Ile Phe Asp Lys Val Glu Phe 
    290                 295                 300                 


Asp Gly Pro Glu Lys Ala Gly Leu Ile Val His Tyr Ile Trp Gln Leu 
305                 310                 315                 320 


Ala Ile Pro Tyr Phe Cys Asn Met Ser Leu Phe Glu Gly Val Ala Tyr 
                325                 330                 335     


Phe Leu Met Gly Gln Ala Ser Cys Gly Leu Leu Leu Ala Leu Val Phe 
            340                 345                 350         


Ser Ile Gly His Asn Gly Met Ser Val Tyr Glu Arg Glu Thr Lys Pro 
        355                 360                 365             


Asp Phe Trp Gln Leu Gln Val Thr Thr Thr Arg Asn Ile Arg Ala Ser 
    370                 375                 380                 


Val Phe Met Asp Trp Phe Thr Gly Gly Leu Asn Tyr Gln Ile Asp His 
385                 390                 395                 400 


His Leu Phe Pro Leu Val Pro Arg His Asn Leu Pro Lys Val Asn Val 
                405                 410                 415     


Leu Ile Lys Ser Leu Cys Lys Glu Phe Asp Ile Pro Phe His Glu Thr 
            420                 425                 430         


Gly Phe Trp Glu Gly Ile Tyr Glu Val Val Asp His Leu Ala Asp Ile 
        435                 440                 445             


Ser Lys Glu Phe Ile Thr Glu Phe Pro Ala Met 
    450                 455                 


<210>  3

<211>  831

<212>  DNA

<213>  Traustochytrium sp.



<220>

<221>  CDS

<222>  (1)..(831)

<223>  Delta-6-Elongase



<400>  3
atg gac gtc gtc gag cag caa tgg cgc cgc ttc gtg gac gcc gtg gac       48
Met Asp Val Val Glu Gln Gln Trp Arg Arg Phe Val Asp Ala Val Asp         
1               5                   10                  15              

aac gga atc gtg gag ttc atg gag cat gag aag ccc aac aag ctg aac       96
Asn Gly Ile Val Glu Phe Met Glu His Glu Lys Pro Asn Lys Leu Asn         
            20                  25                  30                  

gag ggc aag ctc ttc acc tcg acc gag gag atg atg gcg ctt atc gtc      144
Glu Gly Lys Leu Phe Thr Ser Thr Glu Glu Met Met Ala Leu Ile Val         
        35                  40                  45                      

ggc tac ctg gcg ttc gtg gtc ctc ggg tcc gcc ttc atg aag gcc ttt      192
Gly Tyr Leu Ala Phe Val Val Leu Gly Ser Ala Phe Met Lys Ala Phe         
    50                  55                  60                          

gtc gat aag cct ttc gag ctc aag ttc ctc aag ctc gtg cac aac atc      240
Val Asp Lys Pro Phe Glu Leu Lys Phe Leu Lys Leu Val His Asn Ile         
65                  70                  75                  80          

ttc ctc acc ggt ctg tcc atg tac atg gcc acc gag tgc gcg cgc cag      288
Phe Leu Thr Gly Leu Ser Met Tyr Met Ala Thr Glu Cys Ala Arg Gln         
                85                  90                  95              

gca tac ctc ggc ggc tac aag ctc ttt ggc aac ccg atg gag aag ggc      336
Ala Tyr Leu Gly Gly Tyr Lys Leu Phe Gly Asn Pro Met Glu Lys Gly         
            100                 105                 110                 

acc gag tcg cac gcc ccg ggc atg gcc aac atc atc tac atc ttc tac      384
Thr Glu Ser His Ala Pro Gly Met Ala Asn Ile Ile Tyr Ile Phe Tyr         
        115                 120                 125                     

gtg agc aag ttc ctc gaa ttc ctc gac acc gtc ttc atg atc ctc ggc      432
Val Ser Lys Phe Leu Glu Phe Leu Asp Thr Val Phe Met Ile Leu Gly         
    130                 135                 140                         

aag aag tgg aag cag ctc agc ttt ctc cac gtc tac cac cac gcg agc      480
Lys Lys Trp Lys Gln Leu Ser Phe Leu His Val Tyr His His Ala Ser         
145                 150                 155                 160         

atc agc ttc atc tgg ggc atc atc gcc cgc ttc gcg ccc ggt ggc gac      528
Ile Ser Phe Ile Trp Gly Ile Ile Ala Arg Phe Ala Pro Gly Gly Asp         
                165                 170                 175             

gcc tac ttc tct acc atc ctc aac agc agc gtg cat gtc gtg ctc tac      576
Ala Tyr Phe Ser Thr Ile Leu Asn Ser Ser Val His Val Val Leu Tyr         
            180                 185                 190                 

ggc tac tac gcc tcg acc acc ctc ggc tac acc ttc atg cgc ccg ctg      624
Gly Tyr Tyr Ala Ser Thr Thr Leu Gly Tyr Thr Phe Met Arg Pro Leu         
        195                 200                 205                     

cgc ccg tac att acc acc att cag ctc acg cag ttc atg gcc atg gtc      672
Arg Pro Tyr Ile Thr Thr Ile Gln Leu Thr Gln Phe Met Ala Met Val         
    210                 215                 220                         

gtc cag tcc gtc tat gac tac tac aac ccc tgc gac tac ccg cag ccc      720
Val Gln Ser Val Tyr Asp Tyr Tyr Asn Pro Cys Asp Tyr Pro Gln Pro         
225                 230                 235                 240         

ctc gtc aag ctg ctc ttc tgg tac atg ctc acc atg ctc ggc ctc ttc      768
Leu Val Lys Leu Leu Phe Trp Tyr Met Leu Thr Met Leu Gly Leu Phe         
                245                 250                 255             

ggc aac ttc ttc gtg cag cag tac ctc aag ccc aag gcg ccc aag aag      816
Gly Asn Phe Phe Val Gln Gln Tyr Leu Lys Pro Lys Ala Pro Lys Lys         
            260                 265                 270                 

cag aag acc atc taa                                                  831
Gln Lys Thr Ile                                                         
        275                                                             


<210>  4

<211>  276

<212>  PRT

<213>  Traustochytrium sp.



<400>  4

Met Asp Val Val Glu Gln Gln Trp Arg Arg Phe Val Asp Ala Val Asp 
1               5                   10                  15      


Asn Gly Ile Val Glu Phe Met Glu His Glu Lys Pro Asn Lys Leu Asn 
            20                  25                  30          


Glu Gly Lys Leu Phe Thr Ser Thr Glu Glu Met Met Ala Leu Ile Val 
        35                  40                  45              


Gly Tyr Leu Ala Phe Val Val Leu Gly Ser Ala Phe Met Lys Ala Phe 
    50                  55                  60                  


Val Asp Lys Pro Phe Glu Leu Lys Phe Leu Lys Leu Val His Asn Ile 
65                  70                  75                  80  


Phe Leu Thr Gly Leu Ser Met Tyr Met Ala Thr Glu Cys Ala Arg Gln 
                85                  90                  95      


Ala Tyr Leu Gly Gly Tyr Lys Leu Phe Gly Asn Pro Met Glu Lys Gly 
            100                 105                 110         


Thr Glu Ser His Ala Pro Gly Met Ala Asn Ile Ile Tyr Ile Phe Tyr 
        115                 120                 125             


Val Ser Lys Phe Leu Glu Phe Leu Asp Thr Val Phe Met Ile Leu Gly 
    130                 135                 140                 


Lys Lys Trp Lys Gln Leu Ser Phe Leu His Val Tyr His His Ala Ser 
145                 150                 155                 160 


Ile Ser Phe Ile Trp Gly Ile Ile Ala Arg Phe Ala Pro Gly Gly Asp 
                165                 170                 175     


Ala Tyr Phe Ser Thr Ile Leu Asn Ser Ser Val His Val Val Leu Tyr 
            180                 185                 190         


Gly Tyr Tyr Ala Ser Thr Thr Leu Gly Tyr Thr Phe Met Arg Pro Leu 
        195                 200                 205             


Arg Pro Tyr Ile Thr Thr Ile Gln Leu Thr Gln Phe Met Ala Met Val 
    210                 215                 220                 


Val Gln Ser Val Tyr Asp Tyr Tyr Asn Pro Cys Asp Tyr Pro Gln Pro 
225                 230                 235                 240 


Leu Val Lys Leu Leu Phe Trp Tyr Met Leu Thr Met Leu Gly Leu Phe 
                245                 250                 255     


Gly Asn Phe Phe Val Gln Gln Tyr Leu Lys Pro Lys Ala Pro Lys Lys 
            260                 265                 270         


Gln Lys Thr Ile 
        275     


<210>  5

<211>  1320

<212>  DNA

<213>  Traustochytrium sp.



<220>

<221>  CDS

<222>  (1)..(1320)

<223>  Delta-5-Desaturase



<400>  5
atg ggc aag ggc agc gag ggc cgc agc gcg gcg cgc gag atg acg gcc       48
Met Gly Lys Gly Ser Glu Gly Arg Ser Ala Ala Arg Glu Met Thr Ala         
1               5                   10                  15              

gag gcg aac ggc gac aag cgg aaa acg att ctg atc gag ggc gtc ctg       96
Glu Ala Asn Gly Asp Lys Arg Lys Thr Ile Leu Ile Glu Gly Val Leu         
            20                  25                  30                  

tac gac gcg acg aac ttt aag cac ccg ggc ggt tcg atc atc aac ttc      144
Tyr Asp Ala Thr Asn Phe Lys His Pro Gly Gly Ser Ile Ile Asn Phe         
        35                  40                  45                      

ttg acc gag ggc gag gcc ggc gtg gac gcg acg cag gcg tac cgc gag      192
Leu Thr Glu Gly Glu Ala Gly Val Asp Ala Thr Gln Ala Tyr Arg Glu         
    50                  55                  60                          

ttt cat cag cgg tcc ggc aag gcc gac aag tac ctc aag tcg ctg ccg      240
Phe His Gln Arg Ser Gly Lys Ala Asp Lys Tyr Leu Lys Ser Leu Pro         
65                  70                  75                  80          

aag ctg gat gcg tcc aag gtg gag tcg cgg ttc tcg gcc aaa gag cag      288
Lys Leu Asp Ala Ser Lys Val Glu Ser Arg Phe Ser Ala Lys Glu Gln         
                85                  90                  95              

gcg cgg cgc gac gcc atg acg cgc gac tac gcg gcc ttt cgc gag gag      336
Ala Arg Arg Asp Ala Met Thr Arg Asp Tyr Ala Ala Phe Arg Glu Glu         
            100                 105                 110                 

ctc gtc gcc gag ggg tac ttt gac ccg tcg atc ccg cac atg att tac      384
Leu Val Ala Glu Gly Tyr Phe Asp Pro Ser Ile Pro His Met Ile Tyr         
        115                 120                 125                     

cgc gtc gtg gag atc gtg gcg ctc ttc gcg ctc tcg ttc tgg ctc atg      432
Arg Val Val Glu Ile Val Ala Leu Phe Ala Leu Ser Phe Trp Leu Met         
    130                 135                 140                         

tcc aag gcc tcg ccc acc tcg ctc gtg ctg ggc gtg gtg atg aac ggc      480
Ser Lys Ala Ser Pro Thr Ser Leu Val Leu Gly Val Val Met Asn Gly         
145                 150                 155                 160         

att gcg cag ggc cgc tgc ggc tgg gtc atg cac gag atg ggc cac ggg      528
Ile Ala Gln Gly Arg Cys Gly Trp Val Met His Glu Met Gly His Gly         
                165                 170                 175             

tcg ttc acg ggc gtc atc tgg ctc gac gac cgg atg tgc gag ttc ttc      576
Ser Phe Thr Gly Val Ile Trp Leu Asp Asp Arg Met Cys Glu Phe Phe         
            180                 185                 190                 

tac ggc gtc ggc tgc ggc atg agc ggg cac tac tgg aag aac cag cac      624
Tyr Gly Val Gly Cys Gly Met Ser Gly His Tyr Trp Lys Asn Gln His         
        195                 200                 205                     

agc aag cac cac gcc gcg ccc aac cgc ctc gag cac gat gtc gat ctc      672
Ser Lys His His Ala Ala Pro Asn Arg Leu Glu His Asp Val Asp Leu         
    210                 215                 220                         

aac acg ctg ccc ctg gtc gcc ttt aac gag cgc gtc gtg cgc aag gtc      720
Asn Thr Leu Pro Leu Val Ala Phe Asn Glu Arg Val Val Arg Lys Val         
225                 230                 235                 240         

aag ccg gga tcg ctg ctg gcg ctc tgg ctg cgc gtg cag gcg tac ctc      768
Lys Pro Gly Ser Leu Leu Ala Leu Trp Leu Arg Val Gln Ala Tyr Leu         
                245                 250                 255             

ttt gcg ccc gtc tcg tgc ctg ctc atc ggc ctt ggc tgg acg ctc tac      816
Phe Ala Pro Val Ser Cys Leu Leu Ile Gly Leu Gly Trp Thr Leu Tyr         
            260                 265                 270                 

ctg cac ccg cgc tac atg ctg cgc acc aag cgg cac atg gag ttc gtc      864
Leu His Pro Arg Tyr Met Leu Arg Thr Lys Arg His Met Glu Phe Val         
        275                 280                 285                     

tgg atc ttc gcg cgc tac att ggc tgg ttc tcg ctc atg ggc gct ctc      912
Trp Ile Phe Ala Arg Tyr Ile Gly Trp Phe Ser Leu Met Gly Ala Leu         
    290                 295                 300                         

ggc tac tcg ccg ggc acc tcg gtc ggg atg tac ctg tgc tcg ttc ggc      960
Gly Tyr Ser Pro Gly Thr Ser Val Gly Met Tyr Leu Cys Ser Phe Gly         
305                 310                 315                 320         

ctc ggc tgc att tac att ttc ctg cag ttc gcc gtc agc cac acg cac     1008
Leu Gly Cys Ile Tyr Ile Phe Leu Gln Phe Ala Val Ser His Thr His         
                325                 330                 335             

ctg ccg gtg acc aac ccg gag gac cag ctg cac tgg ctc gag tac gcg     1056
Leu Pro Val Thr Asn Pro Glu Asp Gln Leu His Trp Leu Glu Tyr Ala         
            340                 345                 350                 

gcc gac cac acg gtg aac att agc acc aag tcc tgg ctc gtc acg tgg     1104
Ala Asp His Thr Val Asn Ile Ser Thr Lys Ser Trp Leu Val Thr Trp         
        355                 360                 365                     

tgg atg tcg aac ctg aac ttt cag atc gag cac cac ctc ttc ccc acg     1152
Trp Met Ser Asn Leu Asn Phe Gln Ile Glu His His Leu Phe Pro Thr         
    370                 375                 380                         

gcg ccg cag ttc cgc ttc aag gaa atc agt cct cgc gtc gag gcc ctc     1200
Ala Pro Gln Phe Arg Phe Lys Glu Ile Ser Pro Arg Val Glu Ala Leu         
385                 390                 395                 400         

ttc aag cgc cac aac ctc ccg tac tac gac ctg ccc tac acg agc gcg     1248
Phe Lys Arg His Asn Leu Pro Tyr Tyr Asp Leu Pro Tyr Thr Ser Ala         
                405                 410                 415             

gtc tcg acc acc ttt gcc aat ctt tat tcc gtc ggc cac tcg gtc ggc     1296
Val Ser Thr Thr Phe Ala Asn Leu Tyr Ser Val Gly His Ser Val Gly         
            420                 425                 430                 

gcc gac acc aag aag cag gac tga                                     1320
Ala Asp Thr Lys Lys Gln Asp                                             
        435                                                             


<210>  6

<211>  439

<212>  PRT

<213>  Traustochytrium sp.



<400>  6

Met Gly Lys Gly Ser Glu Gly Arg Ser Ala Ala Arg Glu Met Thr Ala 
1               5                   10                  15      


Glu Ala Asn Gly Asp Lys Arg Lys Thr Ile Leu Ile Glu Gly Val Leu 
            20                  25                  30          


Tyr Asp Ala Thr Asn Phe Lys His Pro Gly Gly Ser Ile Ile Asn Phe 
        35                  40                  45              


Leu Thr Glu Gly Glu Ala Gly Val Asp Ala Thr Gln Ala Tyr Arg Glu 
    50                  55                  60                  


Phe His Gln Arg Ser Gly Lys Ala Asp Lys Tyr Leu Lys Ser Leu Pro 
65                  70                  75                  80  


Lys Leu Asp Ala Ser Lys Val Glu Ser Arg Phe Ser Ala Lys Glu Gln 
                85                  90                  95      


Ala Arg Arg Asp Ala Met Thr Arg Asp Tyr Ala Ala Phe Arg Glu Glu 
            100                 105                 110         


Leu Val Ala Glu Gly Tyr Phe Asp Pro Ser Ile Pro His Met Ile Tyr 
        115                 120                 125             


Arg Val Val Glu Ile Val Ala Leu Phe Ala Leu Ser Phe Trp Leu Met 
    130                 135                 140                 


Ser Lys Ala Ser Pro Thr Ser Leu Val Leu Gly Val Val Met Asn Gly 
145                 150                 155                 160 


Ile Ala Gln Gly Arg Cys Gly Trp Val Met His Glu Met Gly His Gly 
                165                 170                 175     


Ser Phe Thr Gly Val Ile Trp Leu Asp Asp Arg Met Cys Glu Phe Phe 
            180                 185                 190         


Tyr Gly Val Gly Cys Gly Met Ser Gly His Tyr Trp Lys Asn Gln His 
        195                 200                 205             


Ser Lys His His Ala Ala Pro Asn Arg Leu Glu His Asp Val Asp Leu 
    210                 215                 220                 


Asn Thr Leu Pro Leu Val Ala Phe Asn Glu Arg Val Val Arg Lys Val 
225                 230                 235                 240 


Lys Pro Gly Ser Leu Leu Ala Leu Trp Leu Arg Val Gln Ala Tyr Leu 
                245                 250                 255     


Phe Ala Pro Val Ser Cys Leu Leu Ile Gly Leu Gly Trp Thr Leu Tyr 
            260                 265                 270         


Leu His Pro Arg Tyr Met Leu Arg Thr Lys Arg His Met Glu Phe Val 
        275                 280                 285             


Trp Ile Phe Ala Arg Tyr Ile Gly Trp Phe Ser Leu Met Gly Ala Leu 
    290                 295                 300                 


Gly Tyr Ser Pro Gly Thr Ser Val Gly Met Tyr Leu Cys Ser Phe Gly 
305                 310                 315                 320 


Leu Gly Cys Ile Tyr Ile Phe Leu Gln Phe Ala Val Ser His Thr His 
                325                 330                 335     


Leu Pro Val Thr Asn Pro Glu Asp Gln Leu His Trp Leu Glu Tyr Ala 
            340                 345                 350         


Ala Asp His Thr Val Asn Ile Ser Thr Lys Ser Trp Leu Val Thr Trp 
        355                 360                 365             


Trp Met Ser Asn Leu Asn Phe Gln Ile Glu His His Leu Phe Pro Thr 
    370                 375                 380                 


Ala Pro Gln Phe Arg Phe Lys Glu Ile Ser Pro Arg Val Glu Ala Leu 
385                 390                 395                 400 


Phe Lys Arg His Asn Leu Pro Tyr Tyr Asp Leu Pro Tyr Thr Ser Ala 
                405                 410                 415     


Val Ser Thr Thr Phe Ala Asn Leu Tyr Ser Val Gly His Ser Val Gly 
            420                 425                 430         


Ala Asp Thr Lys Lys Gln Asp 
        435                 


<210>  7

<211>  819

<212>  DNA

<213>  Talassiosira pseudonana 



<220>

<221>  CDS

<222>  (1)..(819)

<223>  Delta-6-Elongase



<400>  7
atg gac gcc tac aac gct gca atg gat aag atc ggt gcc gcc atc atc       48
Met Asp Ala Tyr Asn Ala Ala Met Asp Lys Ile Gly Ala Ala Ile Ile         
1               5                   10                  15              

gat tgg tct gat ccc gat gga aag ttc cgt gcc gat aga gag gac tgg       96
Asp Trp Ser Asp Pro Asp Gly Lys Phe Arg Ala Asp Arg Glu Asp Trp         
            20                  25                  30                  

tgg ctc tgc gac ttc cgt agc gcc atc acc atc gcc ctc atc tac atc      144
Trp Leu Cys Asp Phe Arg Ser Ala Ile Thr Ile Ala Leu Ile Tyr Ile         
        35                  40                  45                      

gcc ttc gtc atc ctc ggt tcc gcc gtc atg caa tcc ctc ccc gca atg      192
Ala Phe Val Ile Leu Gly Ser Ala Val Met Gln Ser Leu Pro Ala Met         
    50                  55                  60                          

gat ccc tac ccc atc aaa ttc ctc tac aac gtc tcc caa atc ttc ctt      240
Asp Pro Tyr Pro Ile Lys Phe Leu Tyr Asn Val Ser Gln Ile Phe Leu         
65                  70                  75                  80          

tgt gcc tac atg act gtc gag gcg gga ttt ttg gcc tac cgc aat gga      288
Cys Ala Tyr Met Thr Val Glu Ala Gly Phe Leu Ala Tyr Arg Asn Gly         
                85                  90                  95              

tat acc gtc atg cct tgc aat cat ttc aat gtg aat gat cct ccc gtg      336
Tyr Thr Val Met Pro Cys Asn His Phe Asn Val Asn Asp Pro Pro Val         
            100                 105                 110                 

gcg aat ctt ctt tgg ttg ttt tat att tcc aag gtg tgg gac ttt tgg      384
Ala Asn Leu Leu Trp Leu Phe Tyr Ile Ser Lys Val Trp Asp Phe Trp         
        115                 120                 125                     

gat acc att ttc att gtg ttg ggg aag aag tgg cgt caa tta tct ttc      432
Asp Thr Ile Phe Ile Val Leu Gly Lys Lys Trp Arg Gln Leu Ser Phe         
    130                 135                 140                         

ttg cat gta tac cat cac acc acc atc ttt cta ttc tat tgg ctg aat      480
Leu His Val Tyr His His Thr Thr Ile Phe Leu Phe Tyr Trp Leu Asn         
145                 150                 155                 160         

gcc aat gtc ttg tac gat ggt gac atc ttc ctt acc atc ttg ctc aat      528
Ala Asn Val Leu Tyr Asp Gly Asp Ile Phe Leu Thr Ile Leu Leu Asn         
                165                 170                 175             

gga ttc atc cac acg gtg atg tac acg tat tac ttc atc tgt atg cat      576
Gly Phe Ile His Thr Val Met Tyr Thr Tyr Tyr Phe Ile Cys Met His         
            180                 185                 190                 

acc aaa gat tcc aag acg ggc aag agt ctt cct ata tgg tgg aag tcg      624
Thr Lys Asp Ser Lys Thr Gly Lys Ser Leu Pro Ile Trp Trp Lys Ser         
        195                 200                 205                     

agt ttg acg gcg ttt cag ttg ttg caa ttc act atc atg atg agt cag      672
Ser Leu Thr Ala Phe Gln Leu Leu Gln Phe Thr Ile Met Met Ser Gln         
    210                 215                 220                         

gct acc tac ctt gtc ttc cac ggg tgt gat aag gtg tcg ctt cgt atc      720
Ala Thr Tyr Leu Val Phe His Gly Cys Asp Lys Val Ser Leu Arg Ile         
225                 230                 235                 240         

acg att gtg tac ttt gtg tcc ctt ttg agt ttg ttc ttc ctt ttt gct      768
Thr Ile Val Tyr Phe Val Ser Leu Leu Ser Leu Phe Phe Leu Phe Ala         
                245                 250                 255             

cag ttc ttt gtg caa tca tac atg gca ccc aaa aag aag aag agt gct      816
Gln Phe Phe Val Gln Ser Tyr Met Ala Pro Lys Lys Lys Lys Ser Ala         
            260                 265                 270                 

tag                                                                  819


<210>  8

<211>  272

<212>  PRT

<213>  Talassiosira pseudonana 



<400>  8

Met Asp Ala Tyr Asn Ala Ala Met Asp Lys Ile Gly Ala Ala Ile Ile 
1               5                   10                  15      


Asp Trp Ser Asp Pro Asp Gly Lys Phe Arg Ala Asp Arg Glu Asp Trp 
            20                  25                  30          


Trp Leu Cys Asp Phe Arg Ser Ala Ile Thr Ile Ala Leu Ile Tyr Ile 
        35                  40                  45              


Ala Phe Val Ile Leu Gly Ser Ala Val Met Gln Ser Leu Pro Ala Met 
    50                  55                  60                  


Asp Pro Tyr Pro Ile Lys Phe Leu Tyr Asn Val Ser Gln Ile Phe Leu 
65                  70                  75                  80  


Cys Ala Tyr Met Thr Val Glu Ala Gly Phe Leu Ala Tyr Arg Asn Gly 
                85                  90                  95      


Tyr Thr Val Met Pro Cys Asn His Phe Asn Val Asn Asp Pro Pro Val 
            100                 105                 110         


Ala Asn Leu Leu Trp Leu Phe Tyr Ile Ser Lys Val Trp Asp Phe Trp 
        115                 120                 125             


Asp Thr Ile Phe Ile Val Leu Gly Lys Lys Trp Arg Gln Leu Ser Phe 
    130                 135                 140                 


Leu His Val Tyr His His Thr Thr Ile Phe Leu Phe Tyr Trp Leu Asn 
145                 150                 155                 160 


Ala Asn Val Leu Tyr Asp Gly Asp Ile Phe Leu Thr Ile Leu Leu Asn 
                165                 170                 175     


Gly Phe Ile His Thr Val Met Tyr Thr Tyr Tyr Phe Ile Cys Met His 
            180                 185                 190         


Thr Lys Asp Ser Lys Thr Gly Lys Ser Leu Pro Ile Trp Trp Lys Ser 
        195                 200                 205             


Ser Leu Thr Ala Phe Gln Leu Leu Gln Phe Thr Ile Met Met Ser Gln 
    210                 215                 220                 


Ala Thr Tyr Leu Val Phe His Gly Cys Asp Lys Val Ser Leu Arg Ile 
225                 230                 235                 240 


Thr Ile Val Tyr Phe Val Ser Leu Leu Ser Leu Phe Phe Leu Phe Ala 
                245                 250                 255     


Gln Phe Phe Val Gln Ser Tyr Met Ala Pro Lys Lys Lys Lys Ser Ala 
            260                 265                 270         


<210>  9

<211>  1086

<212>  DNA

<213>  Phytophthora infestans



<220>

<221>  CDS

<222>  (1)..(1086)

<223>  Omega-3-Desaturase



<400>  9
atg gcg acg aag gag gcg tat gtg ttc ccc act ctg acg gag atc aag       48
Met Ala Thr Lys Glu Ala Tyr Val Phe Pro Thr Leu Thr Glu Ile Lys         
1               5                   10                  15              

cgg tcg cta cct aaa gac tgt ttc gag gct tcg gtg cct ctg tcg ctc       96
Arg Ser Leu Pro Lys Asp Cys Phe Glu Ala Ser Val Pro Leu Ser Leu         
            20                  25                  30                  

tac tac acc gtg cgt tgt ctg gtg atc gcg gtg gct cta acc ttc ggt      144
Tyr Tyr Thr Val Arg Cys Leu Val Ile Ala Val Ala Leu Thr Phe Gly         
        35                  40                  45                      

ctc aac tac gct cgc gct ctg ccc gag gtc gag agc ttc tgg gct ctg      192
Leu Asn Tyr Ala Arg Ala Leu Pro Glu Val Glu Ser Phe Trp Ala Leu         
    50                  55                  60                          

gac gcc gca ctc tgc acg ggc tac atc ttg ctg cag ggc atc gtg ttc      240
Asp Ala Ala Leu Cys Thr Gly Tyr Ile Leu Leu Gln Gly Ile Val Phe         
65                  70                  75                  80          

tgg ggc ttc ttc acg gtg ggc cac gat gcc ggc cac ggc gcc ttc tcg      288
Trp Gly Phe Phe Thr Val Gly His Asp Ala Gly His Gly Ala Phe Ser         
                85                  90                  95              

cgc tac cac ctg ctt aac ttc gtg gtg ggc act ttc atg cac tcg ctc      336
Arg Tyr His Leu Leu Asn Phe Val Val Gly Thr Phe Met His Ser Leu         
            100                 105                 110                 

atc ctc acg ccc ttc gag tcg tgg aag ctc acg cac cgt cac cac cac      384
Ile Leu Thr Pro Phe Glu Ser Trp Lys Leu Thr His Arg His His His         
        115                 120                 125                     

aag aac acg ggc aac att gac cgt gac gag gtc ttc tac ccg caa cgc      432
Lys Asn Thr Gly Asn Ile Asp Arg Asp Glu Val Phe Tyr Pro Gln Arg         
    130                 135                 140                         

aag gcc gac gac cac ccg ctg tct cgc aac ctg att ctg gcg ctc ggg      480
Lys Ala Asp Asp His Pro Leu Ser Arg Asn Leu Ile Leu Ala Leu Gly         
145                 150                 155                 160         

gca gcg tgg ctc gcc tat ttg gtc gag ggc ttc cct cct cgt aag gtc      528
Ala Ala Trp Leu Ala Tyr Leu Val Glu Gly Phe Pro Pro Arg Lys Val         
                165                 170                 175             

aac cac ttc aac ccg ttc gag cct ctg ttc gtg cgt cag gtg tca gct      576
Asn His Phe Asn Pro Phe Glu Pro Leu Phe Val Arg Gln Val Ser Ala         
            180                 185                 190                 

gtg gta atc tct ctt ctc gcc cac ttc ttc gtg gcc gga ctc tcc atc      624
Val Val Ile Ser Leu Leu Ala His Phe Phe Val Ala Gly Leu Ser Ile         
        195                 200                 205                     

tat ctg agc ctc cag ctg ggc ctt aag acg atg gca atc tac tac tat      672
Tyr Leu Ser Leu Gln Leu Gly Leu Lys Thr Met Ala Ile Tyr Tyr Tyr         
    210                 215                 220                         

gga cct gtt ttt gtg ttc ggc agc atg ctg gtc att acc acc ttc cta      720
Gly Pro Val Phe Val Phe Gly Ser Met Leu Val Ile Thr Thr Phe Leu         
225                 230                 235                 240         

cac cac aat gat gag gag acc cca tgg tac gcc gac tcg gag tgg acg      768
His His Asn Asp Glu Glu Thr Pro Trp Tyr Ala Asp Ser Glu Trp Thr         
                245                 250                 255             

tac gtc aag ggc aac ctc tcg tcc gtg gac cga tcg tac ggc gcg ctc      816
Tyr Val Lys Gly Asn Leu Ser Ser Val Asp Arg Ser Tyr Gly Ala Leu         
            260                 265                 270                 

att gac aac ctg agc cac aac atc ggc acg cac cag atc cac cac ctt      864
Ile Asp Asn Leu Ser His Asn Ile Gly Thr His Gln Ile His His Leu         
        275                 280                 285                     

ttc cct atc att ccg cac tac aaa ctc aag aaa gcc act gcg gcc ttc      912
Phe Pro Ile Ile Pro His Tyr Lys Leu Lys Lys Ala Thr Ala Ala Phe         
    290                 295                 300                         

cac cag gct ttc cct gag ctc gtg cgc aag agc gac gag cca att atc      960
His Gln Ala Phe Pro Glu Leu Val Arg Lys Ser Asp Glu Pro Ile Ile         
305                 310                 315                 320         

aag gct ttc ttc cgg gtt gga cgt ctc tac gca aac tac ggc gtt gtg     1008
Lys Ala Phe Phe Arg Val Gly Arg Leu Tyr Ala Asn Tyr Gly Val Val         
                325                 330                 335             

gac cag gag gcg aag ctc ttc acg cta aag gaa gcc aag gcg gcg acc     1056
Asp Gln Glu Ala Lys Leu Phe Thr Leu Lys Glu Ala Lys Ala Ala Thr         
            340                 345                 350                 

gag gcg gcg gcc aag acc aag tcc acg taa                             1086
Glu Ala Ala Ala Lys Thr Lys Ser Thr                                     
        355                 360                                         


<210>  10

<211>  361

<212>  PRT

<213>  Phytophthora infestans



<400>  10

Met Ala Thr Lys Glu Ala Tyr Val Phe Pro Thr Leu Thr Glu Ile Lys 
1               5                   10                  15      


Arg Ser Leu Pro Lys Asp Cys Phe Glu Ala Ser Val Pro Leu Ser Leu 
            20                  25                  30          


Tyr Tyr Thr Val Arg Cys Leu Val Ile Ala Val Ala Leu Thr Phe Gly 
        35                  40                  45              


Leu Asn Tyr Ala Arg Ala Leu Pro Glu Val Glu Ser Phe Trp Ala Leu 
    50                  55                  60                  


Asp Ala Ala Leu Cys Thr Gly Tyr Ile Leu Leu Gln Gly Ile Val Phe 
65                  70                  75                  80  


Trp Gly Phe Phe Thr Val Gly His Asp Ala Gly His Gly Ala Phe Ser 
                85                  90                  95      


Arg Tyr His Leu Leu Asn Phe Val Val Gly Thr Phe Met His Ser Leu 
            100                 105                 110         


Ile Leu Thr Pro Phe Glu Ser Trp Lys Leu Thr His Arg His His His 
        115                 120                 125             


Lys Asn Thr Gly Asn Ile Asp Arg Asp Glu Val Phe Tyr Pro Gln Arg 
    130                 135                 140                 


Lys Ala Asp Asp His Pro Leu Ser Arg Asn Leu Ile Leu Ala Leu Gly 
145                 150                 155                 160 


Ala Ala Trp Leu Ala Tyr Leu Val Glu Gly Phe Pro Pro Arg Lys Val 
                165                 170                 175     


Asn His Phe Asn Pro Phe Glu Pro Leu Phe Val Arg Gln Val Ser Ala 
            180                 185                 190         


Val Val Ile Ser Leu Leu Ala His Phe Phe Val Ala Gly Leu Ser Ile 
        195                 200                 205             


Tyr Leu Ser Leu Gln Leu Gly Leu Lys Thr Met Ala Ile Tyr Tyr Tyr 
    210                 215                 220                 


Gly Pro Val Phe Val Phe Gly Ser Met Leu Val Ile Thr Thr Phe Leu 
225                 230                 235                 240 


His His Asn Asp Glu Glu Thr Pro Trp Tyr Ala Asp Ser Glu Trp Thr 
                245                 250                 255     


Tyr Val Lys Gly Asn Leu Ser Ser Val Asp Arg Ser Tyr Gly Ala Leu 
            260                 265                 270         


Ile Asp Asn Leu Ser His Asn Ile Gly Thr His Gln Ile His His Leu 
        275                 280                 285             


Phe Pro Ile Ile Pro His Tyr Lys Leu Lys Lys Ala Thr Ala Ala Phe 
    290                 295                 300                 


His Gln Ala Phe Pro Glu Leu Val Arg Lys Ser Asp Glu Pro Ile Ile 
305                 310                 315                 320 


Lys Ala Phe Phe Arg Val Gly Arg Leu Tyr Ala Asn Tyr Gly Val Val 
                325                 330                 335     


Asp Gln Glu Ala Lys Leu Phe Thr Leu Lys Glu Ala Lys Ala Ala Thr 
            340                 345                 350         


Glu Ala Ala Ala Lys Thr Lys Ser Thr 
        355                 360     


<210>  11

<211>  755

<212>  DNA

<213>  35S-Virus



<220>

<221>  promoter

<222>  (1)..(755)

<223>  



<400>  11
cctgcaggtc aacatggtgg agcacgacac acttgtctac tccaaaaata tcaaagatac     60

agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat ccggaaacct    120

cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg aaaaggaagg    180

tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag atgcctctgc    240

cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt    300

tccaaccacg tcttcaaagc aagtggattg atgtgataac atggtggagc acgacacact    360

tgtctactcc aaaaatatca aagatacagt ctcagaagac caaagggcaa ttgagacttt    420

tcaacaaagg gtaatatccg gaaacctcct cggattccat tgcccagcta tctgtcactt    480

tattgtgaag atagtggaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg    540

aaaggccatc gttgaagatg cctctgccga cagtggtccc aaagatggac ccccacccac    600

gaggagcatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg    660

tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc    720

ctctatataa ggaagttcat ttcatttgga gagga                               755


<210>  12

<211>  211

<212>  DNA

<213>  35S-Virus



<220>

<221>  terminator

<222>  (1)..(211)

<223>  



<400>  12
agtccgcaaa aatcaccagt ctctctctac aaatctatct ctctctattt ttctccagaa     60

taatgtgtga gtagttccca gataagggaa ttagggttct tatagggttt cgctcatgtg    120

ttgagcatat aagaaaccct tagtatgtat ttgtatttgt aaaatacttc tatcaataaa    180

atttctaatt cctaaaacca aaatccagtg a                                   211


