                         SEQUENCE LISTING

<110>  BASF Plant Science GmbH
 
<120>  Selection system for maize

<130>  AE20050616 / PF 56914 US

<160>  22    

<170>  PatentIn version 3.3

<210>  1
<211>  1329
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(1329)
<223>  E.coli D-serine dehydratase [dsdA] gene/CDS

<400>  1
atg gaa aac gct aaa atg aac tcg ctc atc gcc cag tat ccg ttg gta       48
Met Glu Asn Ala Lys Met Asn Ser Leu Ile Ala Gln Tyr Pro Leu Val         
1               5                   10                  15              

aag gat ctg gtt gct ctt aaa gaa acc acc tgg ttt aat cct ggc acg       96
Lys Asp Leu Val Ala Leu Lys Glu Thr Thr Trp Phe Asn Pro Gly Thr         
            20                  25                  30                  

acc tca ttg gct gaa ggt tta cct tat gtt ggc ctg acc gaa cag gat      144
Thr Ser Leu Ala Glu Gly Leu Pro Tyr Val Gly Leu Thr Glu Gln Asp         
        35                  40                  45                      

gtt cag gac gcc cat gcg cgc tta tcc cgt ttt gca ccc tat ctg gca      192
Val Gln Asp Ala His Ala Arg Leu Ser Arg Phe Ala Pro Tyr Leu Ala         
    50                  55                  60                          

aaa gca ttt cct gaa act gct gcc act ggg ggg att att gaa tca gaa      240
Lys Ala Phe Pro Glu Thr Ala Ala Thr Gly Gly Ile Ile Glu Ser Glu         
65                  70                  75                  80          

ctg gtt gcc att cca gct atg caa aaa cgg ctg gaa aaa gaa tat cag      288
Leu Val Ala Ile Pro Ala Met Gln Lys Arg Leu Glu Lys Glu Tyr Gln         
                85                  90                  95              

caa ccg atc agc ggg caa ctg tta ctg aaa aaa gat agc cat ttg ccc      336
Gln Pro Ile Ser Gly Gln Leu Leu Leu Lys Lys Asp Ser His Leu Pro         
            100                 105                 110                 

att tcc ggc tcc ata aaa gca cgc ggc ggg att tat gaa gtc ctg gca      384
Ile Ser Gly Ser Ile Lys Ala Arg Gly Gly Ile Tyr Glu Val Leu Ala         
        115                 120                 125                     

cac gca gaa aaa ctg gct ctg gaa gcg ggg ttg ctg acg ctt gat gat      432
His Ala Glu Lys Leu Ala Leu Glu Ala Gly Leu Leu Thr Leu Asp Asp         
    130                 135                 140                         

gac tac agc aaa ctg ctt tct ccg gag ttt aaa cag ttc ttt agc caa      480
Asp Tyr Ser Lys Leu Leu Ser Pro Glu Phe Lys Gln Phe Phe Ser Gln         
145                 150                 155                 160         

tac agc att gct gtg ggc tca acc gga aat ctg ggg tta tca atc ggc      528
Tyr Ser Ile Ala Val Gly Ser Thr Gly Asn Leu Gly Leu Ser Ile Gly         
                165                 170                 175             

att atg agc gcc cgc att ggc ttt aag gtg aca gtt cat atg tct gct      576
Ile Met Ser Ala Arg Ile Gly Phe Lys Val Thr Val His Met Ser Ala         
            180                 185                 190                 

gat gcc cgg gca tgg aaa aaa gcg aaa ctg cgc agc cat ggc gtt acg      624
Asp Ala Arg Ala Trp Lys Lys Ala Lys Leu Arg Ser His Gly Val Thr         
        195                 200                 205                     

gtc gtg gaa tat gag caa gat tat ggt gtt gcc gtc gag gaa gga cgt      672
Val Val Glu Tyr Glu Gln Asp Tyr Gly Val Ala Val Glu Glu Gly Arg         
    210                 215                 220                         

aaa gca gcg cag tct gac ccg aac tgt ttc ttt att gat gac gaa aat      720
Lys Ala Ala Gln Ser Asp Pro Asn Cys Phe Phe Ile Asp Asp Glu Asn         
225                 230                 235                 240         

tcc cgc acg ttg ttc ctt ggg tat tcc gtc gct ggc cag cgt ctt aaa      768
Ser Arg Thr Leu Phe Leu Gly Tyr Ser Val Ala Gly Gln Arg Leu Lys         
                245                 250                 255             

gcg caa ttt gcc cag caa ggc cgt atc gtc gat gct gat aac cct ctg      816
Ala Gln Phe Ala Gln Gln Gly Arg Ile Val Asp Ala Asp Asn Pro Leu         
            260                 265                 270                 

ttt gtc tat ctg ccg tgt ggt gtt ggc ggt ggt cct ggt ggc gtc gca      864
Phe Val Tyr Leu Pro Cys Gly Val Gly Gly Gly Pro Gly Gly Val Ala         
        275                 280                 285                     

ttc ggg ctt aaa ctg gcg ttt ggc gat cat gtt cac tgc ttt ttt gcc      912
Phe Gly Leu Lys Leu Ala Phe Gly Asp His Val His Cys Phe Phe Ala         
    290                 295                 300                         

gaa cca acg cac tcc cct tgt atg ttg tta ggc gtc cat aca gga tta      960
Glu Pro Thr His Ser Pro Cys Met Leu Leu Gly Val His Thr Gly Leu         
305                 310                 315                 320         

cac gat cag att tct gtt cag gat att ggt atc gac aac ctt acc gca     1008
His Asp Gln Ile Ser Val Gln Asp Ile Gly Ile Asp Asn Leu Thr Ala         
                325                 330                 335             

gcg gat ggc ctt gca gtt ggt cgc gca tca ggc ttt gtc ggg cgg gca     1056
Ala Asp Gly Leu Ala Val Gly Arg Ala Ser Gly Phe Val Gly Arg Ala         
            340                 345                 350                 

atg gag cgt ctg ctg gat ggc ttc tat acc ctt agc gat caa acc atg     1104
Met Glu Arg Leu Leu Asp Gly Phe Tyr Thr Leu Ser Asp Gln Thr Met         
        355                 360                 365                     

tat gac atg ctt ggc tgg ctg gcg cag gaa gaa ggt att cgt ctt gaa     1152
Tyr Asp Met Leu Gly Trp Leu Ala Gln Glu Glu Gly Ile Arg Leu Glu         
    370                 375                 380                         

cct tcg gca ctg gcg ggt atg gcc gga cct cag cgc gtg tgt gca tca     1200
Pro Ser Ala Leu Ala Gly Met Ala Gly Pro Gln Arg Val Cys Ala Ser         
385                 390                 395                 400         

gta agt tac caa cag atg cac ggt ttc agc gca gaa caa ctg cgt aat     1248
Val Ser Tyr Gln Gln Met His Gly Phe Ser Ala Glu Gln Leu Arg Asn         
                405                 410                 415             

acc act cat ctg gtg tgg gcg acg gga ggt gga atg gtg ccg gaa gaa     1296
Thr Thr His Leu Val Trp Ala Thr Gly Gly Gly Met Val Pro Glu Glu         
            420                 425                 430                 

gag atg aat caa tat ctg gca aaa ggc cgt taa                         1329
Glu Met Asn Gln Tyr Leu Ala Lys Gly Arg                                 
        435                 440                                         


<210>  2
<211>  442
<212>  PRT
<213>  Escherichia coli

<400>  2

Met Glu Asn Ala Lys Met Asn Ser Leu Ile Ala Gln Tyr Pro Leu Val 
1               5                   10                  15      


Lys Asp Leu Val Ala Leu Lys Glu Thr Thr Trp Phe Asn Pro Gly Thr 
            20                  25                  30          


Thr Ser Leu Ala Glu Gly Leu Pro Tyr Val Gly Leu Thr Glu Gln Asp 
        35                  40                  45              


Val Gln Asp Ala His Ala Arg Leu Ser Arg Phe Ala Pro Tyr Leu Ala 
    50                  55                  60                  


Lys Ala Phe Pro Glu Thr Ala Ala Thr Gly Gly Ile Ile Glu Ser Glu 
65                  70                  75                  80  


Leu Val Ala Ile Pro Ala Met Gln Lys Arg Leu Glu Lys Glu Tyr Gln 
                85                  90                  95      


Gln Pro Ile Ser Gly Gln Leu Leu Leu Lys Lys Asp Ser His Leu Pro 
            100                 105                 110         


Ile Ser Gly Ser Ile Lys Ala Arg Gly Gly Ile Tyr Glu Val Leu Ala 
        115                 120                 125             


His Ala Glu Lys Leu Ala Leu Glu Ala Gly Leu Leu Thr Leu Asp Asp 
    130                 135                 140                 


Asp Tyr Ser Lys Leu Leu Ser Pro Glu Phe Lys Gln Phe Phe Ser Gln 
145                 150                 155                 160 


Tyr Ser Ile Ala Val Gly Ser Thr Gly Asn Leu Gly Leu Ser Ile Gly 
                165                 170                 175     


Ile Met Ser Ala Arg Ile Gly Phe Lys Val Thr Val His Met Ser Ala 
            180                 185                 190         


Asp Ala Arg Ala Trp Lys Lys Ala Lys Leu Arg Ser His Gly Val Thr 
        195                 200                 205             


Val Val Glu Tyr Glu Gln Asp Tyr Gly Val Ala Val Glu Glu Gly Arg 
    210                 215                 220                 


Lys Ala Ala Gln Ser Asp Pro Asn Cys Phe Phe Ile Asp Asp Glu Asn 
225                 230                 235                 240 


Ser Arg Thr Leu Phe Leu Gly Tyr Ser Val Ala Gly Gln Arg Leu Lys 
                245                 250                 255     


Ala Gln Phe Ala Gln Gln Gly Arg Ile Val Asp Ala Asp Asn Pro Leu 
            260                 265                 270         


Phe Val Tyr Leu Pro Cys Gly Val Gly Gly Gly Pro Gly Gly Val Ala 
        275                 280                 285             


Phe Gly Leu Lys Leu Ala Phe Gly Asp His Val His Cys Phe Phe Ala 
    290                 295                 300                 


Glu Pro Thr His Ser Pro Cys Met Leu Leu Gly Val His Thr Gly Leu 
305                 310                 315                 320 


His Asp Gln Ile Ser Val Gln Asp Ile Gly Ile Asp Asn Leu Thr Ala 
                325                 330                 335     


Ala Asp Gly Leu Ala Val Gly Arg Ala Ser Gly Phe Val Gly Arg Ala 
            340                 345                 350         


Met Glu Arg Leu Leu Asp Gly Phe Tyr Thr Leu Ser Asp Gln Thr Met 
        355                 360                 365             


Tyr Asp Met Leu Gly Trp Leu Ala Gln Glu Glu Gly Ile Arg Leu Glu 
    370                 375                 380                 


Pro Ser Ala Leu Ala Gly Met Ala Gly Pro Gln Arg Val Cys Ala Ser 
385                 390                 395                 400 


Val Ser Tyr Gln Gln Met His Gly Phe Ser Ala Glu Gln Leu Arg Asn 
                405                 410                 415     


Thr Thr His Leu Val Trp Ala Thr Gly Gly Gly Met Val Pro Glu Glu 
            420                 425                 430         


Glu Met Asn Gln Tyr Leu Ala Lys Gly Arg 
        435                 440         


<210>  3
<211>  1107
<212>  DNA
<213>  Rhodosporidium toruloides


<220>
<221>  CDS
<222>  (1)..(1107)
<223>  Rhodosporidium toruloides D-amino acid oxidase CDS

<400>  3
atg cac tcg cag aag cgc gtc gtt gtc ctc gga tca ggc gtt atc ggt       48
Met His Ser Gln Lys Arg Val Val Val Leu Gly Ser Gly Val Ile Gly         
1               5                   10                  15              

ctg agc agc gcc ctc atc ctc gct cgg aag ggc tac agc gtg cat att       96
Leu Ser Ser Ala Leu Ile Leu Ala Arg Lys Gly Tyr Ser Val His Ile         
            20                  25                  30                  

ctc gcg cgc gac ttg ccg gag gac gtc tcg agc cag act ttc gct tca      144
Leu Ala Arg Asp Leu Pro Glu Asp Val Ser Ser Gln Thr Phe Ala Ser         
        35                  40                  45                      

cca tgg gct ggc gcg aat tgg acg cct ttc atg acg ctt aca gac ggt      192
Pro Trp Ala Gly Ala Asn Trp Thr Pro Phe Met Thr Leu Thr Asp Gly         
    50                  55                  60                          

cct cga caa gca aaa tgg gaa gaa tcg act ttc aag aag tgg gtc gag      240
Pro Arg Gln Ala Lys Trp Glu Glu Ser Thr Phe Lys Lys Trp Val Glu         
65                  70                  75                  80          

ttg gtc ccg acg ggc cat gcc atg tgg ctc aag ggg acg agg cgg ttc      288
Leu Val Pro Thr Gly His Ala Met Trp Leu Lys Gly Thr Arg Arg Phe         
                85                  90                  95              

gcg cag aac gaa gac ggc ttg ctc ggg cac tgg tac aag gac atc acg      336
Ala Gln Asn Glu Asp Gly Leu Leu Gly His Trp Tyr Lys Asp Ile Thr         
            100                 105                 110                 

cca aat tac cgc ccc ctc cca tct tcc gaa tgt cca cct ggc gct atc      384
Pro Asn Tyr Arg Pro Leu Pro Ser Ser Glu Cys Pro Pro Gly Ala Ile         
        115                 120                 125                     

ggc gta acc tac gac acc ctc tcc gtc cac gca cca aag tac tgc cag      432
Gly Val Thr Tyr Asp Thr Leu Ser Val His Ala Pro Lys Tyr Cys Gln         
    130                 135                 140                         

tac ctt gca aga gag ctg cag aag ctc ggc gcg acg ttt gag aga cgg      480
Tyr Leu Ala Arg Glu Leu Gln Lys Leu Gly Ala Thr Phe Glu Arg Arg         
145                 150                 155                 160         

acc gtt acg tcg ctt gag cag gcg ttc gac ggt gcg gat ttg gtg gtc      528
Thr Val Thr Ser Leu Glu Gln Ala Phe Asp Gly Ala Asp Leu Val Val         
                165                 170                 175             

aac gct acg gga ctt ggc gcc aag tcg att gcg ggc atc gac gac caa      576
Asn Ala Thr Gly Leu Gly Ala Lys Ser Ile Ala Gly Ile Asp Asp Gln         
            180                 185                 190                 

gcc gcc gag cca atc cgc ggg caa acc gtc ctc gtc aag tcc cca tgc      624
Ala Ala Glu Pro Ile Arg Gly Gln Thr Val Leu Val Lys Ser Pro Cys         
        195                 200                 205                     

aag cga tgc acg atg gac tcg tcc gac ccc gct tct ccc gcc tac atc      672
Lys Arg Cys Thr Met Asp Ser Ser Asp Pro Ala Ser Pro Ala Tyr Ile         
    210                 215                 220                         

att ccc cga cca ggt ggc gaa gtc atc tgc ggc ggg acg tac ggc gtg      720
Ile Pro Arg Pro Gly Gly Glu Val Ile Cys Gly Gly Thr Tyr Gly Val         
225                 230                 235                 240         

gga gac tgg gac ttg tct gtc aac cca gag acg gtc cag cgg atc ctc      768
Gly Asp Trp Asp Leu Ser Val Asn Pro Glu Thr Val Gln Arg Ile Leu         
                245                 250                 255             

aag cac tgc ttg cgc ctc gac ccg acc atc tcg agc gac gga acg atc      816
Lys His Cys Leu Arg Leu Asp Pro Thr Ile Ser Ser Asp Gly Thr Ile         
            260                 265                 270                 

gaa ggc atc gag gtc ctc cgc cac aac gtc ggc ttg cga cct gca cga      864
Glu Gly Ile Glu Val Leu Arg His Asn Val Gly Leu Arg Pro Ala Arg         
        275                 280                 285                     

cga ggc gga ccc cgc gtt gag gca gaa cgg atc gtc ctg cct ctc gac      912
Arg Gly Gly Pro Arg Val Glu Ala Glu Arg Ile Val Leu Pro Leu Asp         
    290                 295                 300                         

cgg aca aag tcg ccc ctc tcg ctc ggc agg ggc agc gca cga gcg gcg      960
Arg Thr Lys Ser Pro Leu Ser Leu Gly Arg Gly Ser Ala Arg Ala Ala         
305                 310                 315                 320         

aag gag aag gag gtc acg ctt gtg cat gcg tat ggc ttc tcg agt gcg     1008
Lys Glu Lys Glu Val Thr Leu Val His Ala Tyr Gly Phe Ser Ser Ala         
                325                 330                 335             

gga tac cag cag agt tgg ggc gcg gcg gag gat gtc gcg cag ctc gtc     1056
Gly Tyr Gln Gln Ser Trp Gly Ala Ala Glu Asp Val Ala Gln Leu Val         
            340                 345                 350                 

gac gag gcg ttc cag cgg tac cac ggc gcg gcg cgg gag tcg aag ttg     1104
Asp Glu Ala Phe Gln Arg Tyr His Gly Ala Ala Arg Glu Ser Lys Leu         
        355                 360                 365                     

tag                                                                 1107


<210>  4
<211>  368
<212>  PRT
<213>  Rhodosporidium toruloides

<400>  4

Met His Ser Gln Lys Arg Val Val Val Leu Gly Ser Gly Val Ile Gly 
1               5                   10                  15      


Leu Ser Ser Ala Leu Ile Leu Ala Arg Lys Gly Tyr Ser Val His Ile 
            20                  25                  30          


Leu Ala Arg Asp Leu Pro Glu Asp Val Ser Ser Gln Thr Phe Ala Ser 
        35                  40                  45              


Pro Trp Ala Gly Ala Asn Trp Thr Pro Phe Met Thr Leu Thr Asp Gly 
    50                  55                  60                  


Pro Arg Gln Ala Lys Trp Glu Glu Ser Thr Phe Lys Lys Trp Val Glu 
65                  70                  75                  80  


Leu Val Pro Thr Gly His Ala Met Trp Leu Lys Gly Thr Arg Arg Phe 
                85                  90                  95      


Ala Gln Asn Glu Asp Gly Leu Leu Gly His Trp Tyr Lys Asp Ile Thr 
            100                 105                 110         


Pro Asn Tyr Arg Pro Leu Pro Ser Ser Glu Cys Pro Pro Gly Ala Ile 
        115                 120                 125             


Gly Val Thr Tyr Asp Thr Leu Ser Val His Ala Pro Lys Tyr Cys Gln 
    130                 135                 140                 


Tyr Leu Ala Arg Glu Leu Gln Lys Leu Gly Ala Thr Phe Glu Arg Arg 
145                 150                 155                 160 


Thr Val Thr Ser Leu Glu Gln Ala Phe Asp Gly Ala Asp Leu Val Val 
                165                 170                 175     


Asn Ala Thr Gly Leu Gly Ala Lys Ser Ile Ala Gly Ile Asp Asp Gln 
            180                 185                 190         


Ala Ala Glu Pro Ile Arg Gly Gln Thr Val Leu Val Lys Ser Pro Cys 
        195                 200                 205             


Lys Arg Cys Thr Met Asp Ser Ser Asp Pro Ala Ser Pro Ala Tyr Ile 
    210                 215                 220                 


Ile Pro Arg Pro Gly Gly Glu Val Ile Cys Gly Gly Thr Tyr Gly Val 
225                 230                 235                 240 


Gly Asp Trp Asp Leu Ser Val Asn Pro Glu Thr Val Gln Arg Ile Leu 
                245                 250                 255     


Lys His Cys Leu Arg Leu Asp Pro Thr Ile Ser Ser Asp Gly Thr Ile 
            260                 265                 270         


Glu Gly Ile Glu Val Leu Arg His Asn Val Gly Leu Arg Pro Ala Arg 
        275                 280                 285             


Arg Gly Gly Pro Arg Val Glu Ala Glu Arg Ile Val Leu Pro Leu Asp 
    290                 295                 300                 


Arg Thr Lys Ser Pro Leu Ser Leu Gly Arg Gly Ser Ala Arg Ala Ala 
305                 310                 315                 320 


Lys Glu Lys Glu Val Thr Leu Val His Ala Tyr Gly Phe Ser Ser Ala 
                325                 330                 335     


Gly Tyr Gln Gln Ser Trp Gly Ala Ala Glu Asp Val Ala Gln Leu Val 
            340                 345                 350         


Asp Glu Ala Phe Gln Arg Tyr His Gly Ala Ala Arg Glu Ser Lys Leu 
        355                 360                 365             


<210>  5
<211>  275
<212>  DNA
<213>  Zea mays


<220>
<221>  promoter
<222>  (1)..(275)
<223>  Zea mays ubiquitin 275 bp core promoter

<220>
<221>  misc_signal
<222>  (61)..(74)
<223>  Similar to consensus heat shock element

<220>
<221>  misc_signal
<222>  (71)..(84)
<223>  Similar to consensus heat shock element

<220>
<221>  TATA_signal
<222>  (246)..(254)
<223>  TATA Box

<400>  5
aaccagcagc gtcgcgtcgg gccaagcgaa gcagacggca cggcatctct gtcgctgcct     60

ctggacccct ctcgagagtt ccgctccacc gttggacttg ctccgctgtc ggcatccaga    120

aattgcgtgg cggagcggca gacgtgagcc ggcacggcag gcggcctcct cctcctctca    180

cggcaccggc agctacgggg gattcctttc ccaccgctcc ttcgctttcc cttcctcgcc    240

cgccgtaata aatagacacc ccctccacac cctct                               275


<210>  6
<211>  1988
<212>  DNA
<213>  Zea mays


<220>
<221>  promoter
<222>  (1)..(1988)
<223>  Zea mays ubiquitin promoter region with 5'UTR and intron

<220>
<221>  promoter
<222>  (1)..(899)
<223>  Zea mays ubiquitin promoter region

<220>
<221>  misc_signal
<222>  (685)..(698)
<223>  Similar to consensus heat shock element

<220>
<221>  misc_signal
<222>  (695)..(708)
<223>  Similar to consensus heat shock element

<220>
<221>  TATA_signal
<222>  (870)..(878)
<223>  TATA Box

<220>
<221>  5'UTR
<222>  (900)..(1988)

<220>
<221>  Intron
<222>  (983)..(1988)
<223>  Zea mays ubiquitin gene intron MubG1

<400>  6
tgcagtgcag cgtgacccgg tcgtgcccct ctctagagat aatgagcatt gcatgtctaa     60

gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca gtttatctat    120

ctttatacat atatttaaac tttactctac gaataatata atctatagta ctacaataat    180

atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag gacaattgag    240

tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt ctcctttttt    300

tttgcaaata gcttcaccta tataatactt catccatttt attagtacat ccatttaggg    360

tttagggtta atggttttta tagactaatt tttttagtac atctatttta ttctatttta    420

gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata gtttagatat    480

aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga aattaaaaaa    540

actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa cgccgtcgac    600

gagtctaacg gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag cgaagcagac    660

ggcacggcat ctctgtcgct gcctctggac ccctctcgag agttccgctc caccgttgga    720

cttgctccgc tgtcggcatc cagaaattgc gtggcggagc ggcagacgtg agccggcacg    780

gcaggcggcc tcctcctcct ctcacggcac cggcagctac gggggattcc tttcccaccg    840

ctccttcgct ttcccttcct cgcccgccgt aataaataga caccccctcc acaccctctt    900

tccccaacct cgtgttgttc ggagcgcaca cacacacaac cagatctccc ccaaatccac    960

ccgtcggcac ctccgcttca aggtacgccg ctcgtcctcc cccccccccc ccctctctac   1020

cttctctaga tcggcgttcc ggtccatggt tagggcccgg tagttctact tctgttcatg   1080

tttgtgttag atccgtgttt gtgttagatc cgtgctgcta gcgttcgtac acggatgcga   1140

cctgtacgtc agacacgttc tgattgctaa cttgccagtg tttctctttg gggaatcctg   1200

ggatggctct agccgttccg cagacgggat cgatttcatg attttttttg tttcgttgca   1260

tagggtttgg tttgcccttt tcctttattt caatatatgc cgtgcacttg tttgtcgggt   1320

catcttttca tgcttttttt tgtcttggtt gtgatgatgt ggtctggttg ggcggtcgtt   1380

ctagatcgga gtagaattct gtttcaaact acctggtgga tttattaatt ttggatctgt   1440

atgtgtgtgc catacatatt catagttacg aattgaagat gatggatgga aatatcgatc   1500

taggataggt atacatgttg atgcgggttt tactgatgca tatacagaga tgctttttgt   1560

tcgcttggtt gtgatgatgt ggtgtggttg ggcggtcgtt cattcgttct agatcggagt   1620

agaatactgt ttcaaactac ctggtgtatt tattaatttt ggaactgtat gtgtgtgtca   1680

tacatcttca tagttacgag tttaagatgg atggaaatat cgatctagga taggtataca   1740

tgttgatgtg ggttttactg atgcatatac atgatggcat atgcagcatc tattcatatg   1800

ctctaacctt gagtacctat ctattataat aaacaagtat gttttataat tatttcgatc   1860

ttgatatact tggatgatgg catatgcagc agctatatgt ggattttttt agccctgcct   1920

tcatacgcta tttatttgct tggtactgtt tcttttgtcg atgctcaccc tgttgtttgg   1980

tgttactt                                                            1988


<210>  7
<211>  275
<212>  DNA
<213>  Sugarcane bacilliform virus


<220>
<221>  promoter
<222>  (1)..(275)
<223>  Sugarcane bacilliform virus 275 bp core promoter

<220>
<221>  TATA_signal
<222>  (224)..(243)
<223>  TATA Box

<400>  7
gattgaggag gcattgacgt cagggatgac cgcagcggag agtactgggc ccattcagtg     60

gatgctccac tgagttgtat tattgtgtgc ttttcggaca agtgtgctgt ccactttctt    120

ttggcacctg tgccacttta ttccttgtct gccacgatgc ctttgcttag cttgtaagca    180

aggatcgcag tgcgtgtgtg acaccacccc ccttccgacg ctctgcctat ataaggcacc    240

gtctgtaagc tcttacgatc atcggtagtt cacca                               275


<210>  8
<211>  1398
<212>  DNA
<213>  Sugarcane bacilliform virus


<220>
<221>  promoter
<222>  (1)..(1398)
<223>  Sugarcane bacilliform virus [ScBV] promoter region

<220>
<221>  misc_signal
<222>  (98)..(118)
<223>  Heat shock factor

<220>
<221>  misc_signal
<222>  (365)..(385)
<223>  Heat shock factor

<220>
<221>  misc_signal
<222>  (606)..(626)
<223>  Heat shock factor

<220>
<221>  misc_signal
<222>  (705)..(719)
<223>  Heat shock element

<220>
<221>  TATA_signal
<222>  (1345)..(1364)
<223>  TATA Box

<400>  8
aatcctggct agcaacactg aactatgcca gaaaccacat caaagatatg ggcaagcttc     60

ttggcccatt atatccaaag acctcagaga aaggtgagcg aaggctcaat tcagaagatt    120

ggaagctgat caataggatc aagacaatgg tgagaacgct tccaaatctc actattccac    180

cagaagatgc atacattatc attgaaacag atgcatgtgc aactggatgg ggagcagtat    240

gcaagtggaa gaaaaacaag gcagacccaa gaaatacaga gcaaatctgt aggtatgcca    300

gtggaaaatt tgataagcca aaaggaacct gtgatgcaga aatctatggg gttatgaatg    360

gcttagaaaa gatgagattg ttctacttgg acaaaagaga gatcacagtc agaactgaca    420

gtagtgcaat cgaaaggttc tacaacaaga gtgctgaaca caagccttct gagatcagat    480

ggatcaggtt catggactac atcactggtg caggaccaga gatagtcatt gaacacataa    540

aagggaagag caatggttta gctgacatct tgtccaggct caaagccaaa ttagctcaga    600

atgaaccaac ggaagagatg atcctgctta cacaagccat aagggaagta attccttatc    660

cagatcatcc atacactgag caactcagag aatggggaaa caaaattctg gatccattcc    720

ccacattcaa gaaggacatg ttcgaaagaa cagagcaagc ttttatgcta acagaggaac    780

cagttctact ctgtgcatgc aggaagcctg caattcagtt agtgtccaga acatctgcca    840

acccaggaag gaaattcttc aagtgcgcaa tgaacaaatg ccattgctgg tactgggcag    900

atctcattga agaacacatt caagacagaa ttgatgaatt tctcaagaat cttgaagttc    960

tgaagaccgg tggcgtgcaa acaatggagg aggaacttat gaaggaagtc accaagctga   1020

agatagaaga gcaggagttc gaggaatacc aggccacacc aagggctatg tcgccagtag   1080

ccgcagaaga tgtgctagat ctccaagacg taagcaatga cgattgagga ggcattgacg   1140

tcagggatga ccgcagcgga gagtactggg cccattcagt ggatgctcca ctgagttgta   1200

ttattgtgtg cttttcggac aagtgtgctg tccactttct tttggcacct gtgccacttt   1260

attccttgtc tgccacgatg cctttgctta gcttgtaagc aaggatcgca gtgcgtgtgt   1320

gacaccaccc cccttccgac gctctgccta tataaggcac cgtctgtaag ctcttacgat   1380

catcggtagt tcaccaag                                                 1398


<210>  9
<211>  5035
<212>  DNA
<213>  Artificial

<220>
<223>  RLM175 binary vector derived from pSB11


<220>
<221>  misc_structure
<222>  (5)..(132)
<223>  Left T-DNA border from pSB11

<220>
<221>  misc_binding
<222>  (51)..(75)
<223>  Left T-DNA border repeat region from pSB1

<220>
<221>  misc_structure
<222>  (232)..(376)
<223>  Right T-DNA border from pSB11

<220>
<221>  misc_binding
<222>  (272)..(295)
<223>  Right T-DNA border repeat region from pSB1

<220>
<221>  misc_structure
<222>  (777)..(3511)
<223>  pSB1 recombination target

<220>
<221>  rep_origin
<222>  (2654)..(2934)
<223>  ColEI replication origin from pSB11

<220>
<221>  misc_feature
<222>  (3074)..(3334)
<223>  ColEI BOM site from pSB11

<220>
<221>  misc_feature
<222>  (3793)..(4608)
<223>  cds encoding Kanamycin Resistance selection gene from Tn903

<400>  9
agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg tggcaggata     60

tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg gacgttttta    120

atgtactgaa ttggatccgc ccgggcggta ccgaattcgc ggccgcaagc ttgtacaacg    180

cgtaccggtt aattaatcta gaggcgcgcc gggcccggcc ggccagatct tgattgtcgt    240

ttcccgcctt cagtttaaac tatcagtgtt tgacaggata tattggcggg taaacctaag    300

agaaaagagc gtttattaga ataatcggat atttaaaagg gcgtgaaaag gtttatccgt    360

tcgtccattt gtatgtgcat gccaaccaca gggttcccct cgggagtgct tggcattccg    420

tgcgataatg acttctgttc aaccacccaa acgtcggaaa gcctgacgac ggagcagcat    480

tccaaaaaga tcccttggct cgtctgggtc ggctagaagg tcgagtgggc tgctgtggct    540

tgatccctca acgcggtcgc ggacgtagcg cagcgccgaa aaatcctcga tcgcaaatcc    600

gacgctgtcg aaaagcgtga tctgcttgtc gctctttcgg ccgacgtcct ggccagtcat    660

cacgcgccaa agttccgtca caggatgatc tggcgcgagt tgctggatct cgccttcaat    720

ccgggtctgt ggcgggaact ccacgaaaat atccgaacgc agcaagatcg tcgaccaatt    780

cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa    840

tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt    900

tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc    960

ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc   1020

ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa   1080

aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg   1140

gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag   1200

ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc   1260

gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta   1320

cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg   1380

cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca   1440

acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac   1500

caaacgacga gcgtgacacc acgatgccgg gggggggggg gggggacatg aggttgcccc   1560

gtattcagtg tcgctgattt gtattgtctg aagttgtttt tacgttaagt tgatgcagat   1620

caattaatac gatacctgcg tcataattga ttatttgacg tggtttgatg gcctccacgc   1680

acgttgtgat atgtagatga taatcattat cactttacgg gtcctttccg gtgatccgac   1740

aggttacggg gcggcgacct cgcgggtttt cgctatttat gaaaattttc cggtttaagg   1800

cgtttccgtt cttcttcgtc ataacttaat gtttttattt aaaataccct ctgaaaagaa   1860

aggaaacgac aggtgctgaa agcgagcttt ttggcctctg tcgtttcctt tctctgtttt   1920

tgtccgtgga atgaacaatg gaaccccccc cccccccccc tgcagcaatg gcaacaacgt   1980

tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact   2040

ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt   2100

ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg   2160

ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta   2220

tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac   2280

tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta   2340

aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt   2400

tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt   2460

tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt   2520

gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc   2580

agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg   2640

tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg   2700

ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt   2760

cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac   2820

tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg   2880

acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg   2940

gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat   3000

ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt   3060

tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg   3120

attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa   3180

cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc   3240

tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct   3300

ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc   3360

tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc   3420

atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc   3480

gtcatcaccg aaacgcgcga ggcagcagat cccccgatca agtagataca ctacatatat   3540

ctacaataga catcgagccg gaaggtgatg tttactttcc tgaaatcccc agcaatttta   3600

ggccagtttt tacccaagac ttcgcctcta acataaatta tagttaccaa atctggcaaa   3660

agggttgacc gggggggggg ggaaagccac gttgtgtctc aaaatctctg atgttacatt   3720

gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat   3780

acaaggggtg ttatgagcca tattcaacgg gaaacgtctt gctcgaggcc gcgattaaat   3840

tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca   3900

ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat   3960

ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg   4020

gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta   4080

ctcaccactg cgatccccgg gaaaacagca ttccaggtat tagaagaata tcctgattca   4140

ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt   4200

tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg   4260

aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa   4320

caagtctgga aagaaatgca taagcttttg ccattctcac cggattcagt cgtcactcat   4380

ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat   4440

gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc   4500

ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct   4560

gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt   4620

aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgg ctttgttgaa   4680

taaatcgaac ttttgctgag ttgaaggatc agatcacgca tcttcccgac aacgcagacc   4740

gttccgtggc aaagcaaaag ttcaaaatca ccaactggtc cacctacaac aaagctctca   4800

tcaaccgtgg ctccctcact ttctggctgg atgatggggc gattcaggga tcacaggcag   4860

caacgctctg tcatcgttac aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa   4920

acccggcagc ttagttgccg ttcttccgaa tagcatcggt aacatgagca aagtctgccg   4980

ccttacaacg gctctcccgc tgacgccgtc ccggactgat gggctgcctg tatcg        5035


<210>  10
<211>  4503
<212>  DNA
<213>  Artificial

<220>
<223>  T-DNA region from RLM151, a RLM175-type binary vector containing 
       a p-ZmUBI+I::c-dsdA::t-OCS selection cassette.


<220>
<221>  misc_structure
<222>  (1)..(128)
<223>  Left T-DNA border from pSB11

<220>
<221>  misc_binding
<222>  (47)..(71)
<223>  Left T-DNA border repeat from pSB11

<220>
<221>  promoter
<222>  (162)..(2149)
<223>  Zea mays ubiquitin promoter region including intron

<220>
<221>  Intron
<222>  (1144)..(2149)
<223>  Zea mays ubiquitin intron MubG1

<220>
<221>  CDS
<222>  (2192)..(3520)
<223>  E. coli D-serine deaminase [dsdA] CDS

<220>
<221>  terminator
<222>  (3562)..(4270)
<223>  Agrobacterium tumefaciens octapine synthase terminator region

<220>
<221>  misc_structure
<222>  (4359)..(4503)
<223>  Right T-DNA border from pSB11

<220>
<221>  misc_binding
<222>  (4399)..(4422)
<223>  Right T-DNA border repeat from pSB11

<400>  10
gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt     60

gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt    120

actgaattgg atccgcccgg gcggtaccaa gcttccgcgg ctgcagtgca gcgtgacccg    180

gtcgtgcccc tctctagaga taatgagcat tgcatgtcta agttataaaa aattaccaca    240

tatttttttt gtcacacttg tttgaagtgc agtttatcta tctttataca tatatttaaa    300

ctttactcta cgaataatat aatctatagt actacaataa tatcagtgtt ttagagaatc    360

atataaatga acagttagac atggtctaaa ggacaattga gtattttgac aacaggactc    420

tacagtttta tctttttagt gtgcatgtgt tctccttttt ttttgcaaat agcttcacct    480

atataatact tcatccattt tattagtaca tccatttagg gtttagggtt aatggttttt    540

atagactaat ttttttagta catctatttt attctatttt agcctctaaa ttaagaaaac    600

taaaactcta ttttagtttt tttatttaat agtttagata taaaatagaa taaaataaag    660

tgactaaaaa ttaaacaaat accctttaag aaattaaaaa aactaaggaa acatttttct    720

tgtttcgagt agataatgcc agcctgttaa acgccgtcga cgagtctaac ggacaccaac    780

cagcgaacca gcagcgtcgc gtcgggccaa gcgaagcaga cggcacggca tctctgtcgc    840

tgcctctgga cccctctcga gagttccgct ccaccgttgg acttgctccg ctgtcggcat    900

ccagaaattg cgtggcggag cggcagacgt gagccggcac ggcaggcggc ctcctcctcc    960

tctcacggca ccggcagcta cgggggattc ctttcccacc gctccttcgc tttcccttcc   1020

tcgcccgccg taataaatag acaccccctc cacaccctct ttccccaacc tcgtgttgtt   1080

cggagcgcac acacacacaa ccagatctcc cccaaatcca cccgtcggca cctccgcttc   1140

aaggtacgcc gctcgtcctc cccccccccc cccctctcta ccttctctag atcggcgttc   1200

cggtccatgg ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt   1260

tgtgttagat ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt   1320

ctgattgcta acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc   1380

gcagacggga tcgatttcat gatttttttt gtttcgttgc atagggtttg gtttgccctt   1440

ttcctttatt tcaatatatg ccgtgcactt gtttgtcggg tcatcttttc atgctttttt   1500

ttgtcttggt tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtagaattc   1560

tgtttcaaac tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat   1620

tcatagttac gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt   1680

gatgcgggtt ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg   1740

tggtgtggtt gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta   1800

cctggtgtat ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga   1860

gtttaagatg gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact   1920

gatgcatata catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta   1980

tctattataa taaacaagta tgttttataa ttatttcgat cttgatatac ttggatgatg   2040

gcatatgcag cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc   2100

ttggtactgt ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcagggtac   2160

ggatcctcat ctaagcgcaa agagacgtac t atg gaa aac gct aaa atg aac      2212
                                   Met Glu Asn Ala Lys Met Asn          
                                   1               5                    

tcg ctc atc gcc cag tat ccg ttg gta aag gat ctg gtt gct ctt aaa     2260
Ser Leu Ile Ala Gln Tyr Pro Leu Val Lys Asp Leu Val Ala Leu Lys         
        10                  15                  20                      

gaa acc acc tgg ttt aat cct ggc acg acc tca ttg gct gaa ggt tta     2308
Glu Thr Thr Trp Phe Asn Pro Gly Thr Thr Ser Leu Ala Glu Gly Leu         
    25                  30                  35                          

cct tat gtt ggc ctg acc gaa cag gat gtt cag gac gcc cat gcg cgc     2356
Pro Tyr Val Gly Leu Thr Glu Gln Asp Val Gln Asp Ala His Ala Arg         
40                  45                  50                  55          

tta tcc cgt ttt gca ccc tat ctg gca aaa gca ttt cct gaa act gct     2404
Leu Ser Arg Phe Ala Pro Tyr Leu Ala Lys Ala Phe Pro Glu Thr Ala         
                60                  65                  70              

gcc act ggg ggg att att gaa tca gaa ctg gtt gcc att cca gct atg     2452
Ala Thr Gly Gly Ile Ile Glu Ser Glu Leu Val Ala Ile Pro Ala Met         
            75                  80                  85                  

caa aaa cgg ctg gaa aaa gaa tat cag caa ccg atc agc ggg caa ctg     2500
Gln Lys Arg Leu Glu Lys Glu Tyr Gln Gln Pro Ile Ser Gly Gln Leu         
        90                  95                  100                     

tta ctg aaa aaa gat agc cat ttg ccc att tcc ggc tcc ata aaa gca     2548
Leu Leu Lys Lys Asp Ser His Leu Pro Ile Ser Gly Ser Ile Lys Ala         
    105                 110                 115                         

cgc ggc ggg att tat gaa gtc ctg gca cac gca gaa aaa ctg gct ctg     2596
Arg Gly Gly Ile Tyr Glu Val Leu Ala His Ala Glu Lys Leu Ala Leu         
120                 125                 130                 135         

gaa gcg ggg ttg ctg acg ctt gat gat gac tac agc aaa ctg ctt tct     2644
Glu Ala Gly Leu Leu Thr Leu Asp Asp Asp Tyr Ser Lys Leu Leu Ser         
                140                 145                 150             

ccg gag ttt aaa cag ttc ttt agc caa tac agc att gct gtg ggc tca     2692
Pro Glu Phe Lys Gln Phe Phe Ser Gln Tyr Ser Ile Ala Val Gly Ser         
            155                 160                 165                 

acc gga aat ctg ggg tta tca atc ggc att atg agc gcc cgc att ggc     2740
Thr Gly Asn Leu Gly Leu Ser Ile Gly Ile Met Ser Ala Arg Ile Gly         
        170                 175                 180                     

ttt aag gtg aca gtt cat atg tct gct gat gcc cgg gca tgg aaa aaa     2788
Phe Lys Val Thr Val His Met Ser Ala Asp Ala Arg Ala Trp Lys Lys         
    185                 190                 195                         

gcg aaa ctg cgc agc cat ggc gtt acg gtc gtg gaa tat gag caa gat     2836
Ala Lys Leu Arg Ser His Gly Val Thr Val Val Glu Tyr Glu Gln Asp         
200                 205                 210                 215         

tat ggt gtt gcc gtc gag gaa gga cgt aaa gca gcg cag tct gac ccg     2884
Tyr Gly Val Ala Val Glu Glu Gly Arg Lys Ala Ala Gln Ser Asp Pro         
                220                 225                 230             

aac tgt ttc ttt att gat gac gaa aat tcc cgc acg ttg ttc ctt ggg     2932
Asn Cys Phe Phe Ile Asp Asp Glu Asn Ser Arg Thr Leu Phe Leu Gly         
            235                 240                 245                 

tat tcc gtc gct ggc cag cgt ctt aaa gcg caa ttt gcc cag caa ggc     2980
Tyr Ser Val Ala Gly Gln Arg Leu Lys Ala Gln Phe Ala Gln Gln Gly         
        250                 255                 260                     

cgt atc gtc gat gct gat aac cct ctg ttt gtc tat ctg ccg tgt ggt     3028
Arg Ile Val Asp Ala Asp Asn Pro Leu Phe Val Tyr Leu Pro Cys Gly         
    265                 270                 275                         

gtt ggc ggt ggt cct ggt ggc gtc gca ttc ggg ctt aaa ctg gcg ttt     3076
Val Gly Gly Gly Pro Gly Gly Val Ala Phe Gly Leu Lys Leu Ala Phe         
280                 285                 290                 295         

ggc gat cat gtt cac tgc ttt ttt gcc gaa cca acg cac tcc cct tgt     3124
Gly Asp His Val His Cys Phe Phe Ala Glu Pro Thr His Ser Pro Cys         
                300                 305                 310             

atg ttg tta ggc gtc cat aca gga tta cac gat cag att tct gtt cag     3172
Met Leu Leu Gly Val His Thr Gly Leu His Asp Gln Ile Ser Val Gln         
            315                 320                 325                 

gat att ggt atc gac aac ctt acc gca gcg gat ggc ctt gca gtt ggt     3220
Asp Ile Gly Ile Asp Asn Leu Thr Ala Ala Asp Gly Leu Ala Val Gly         
        330                 335                 340                     

cgc gca tca ggc ttt gtc ggg cgg gca atg gag cgt ctg ctg gat ggc     3268
Arg Ala Ser Gly Phe Val Gly Arg Ala Met Glu Arg Leu Leu Asp Gly         
    345                 350                 355                         

ttc tat acc ctt agc gat caa acc atg tat gac atg ctt ggc tgg ctg     3316
Phe Tyr Thr Leu Ser Asp Gln Thr Met Tyr Asp Met Leu Gly Trp Leu         
360                 365                 370                 375         

gcg cag gaa gaa ggt att cgt ctt gaa cct tcg gca ctg gcg ggt atg     3364
Ala Gln Glu Glu Gly Ile Arg Leu Glu Pro Ser Ala Leu Ala Gly Met         
                380                 385                 390             

gcc gga cct cag cgc gtg tgt gca tca gta agt tac caa cag atg cac     3412
Ala Gly Pro Gln Arg Val Cys Ala Ser Val Ser Tyr Gln Gln Met His         
            395                 400                 405                 

ggt ttc agc gca gaa caa ctg cgt aat acc act cat ctg gtg tgg gcg     3460
Gly Phe Ser Ala Glu Gln Leu Arg Asn Thr Thr His Leu Val Trp Ala         
        410                 415                 420                     

acg gga ggt gga atg gtg ccg gaa gaa gag atg aat caa tat ctg gca     3508
Thr Gly Gly Gly Met Val Pro Glu Glu Glu Met Asn Gln Tyr Leu Ala         
    425                 430                 435                         

aaa ggc cgt taa taacgtttca acgcagcatg gatcgtaccg agctcaatcg         3560
Lys Gly Arg                                                             
440                                                                     

atcctgcttt aatgagatat gcgagacgcc tatgatcgca tgatatttgc tttcaattct   3620

gttgtgcacg ttgtaaaaaa cctgagcatg tgtagctcag atccttaccg ccggtttcgg   3680

ttcattctaa tgaatatatc acccgttact atcgtatttt tatgaataat attctccgtt   3740

caatttactg attgtaccct actacttata tgtacaatat taaaatgaaa acaatatatt   3800

gtgctgaata ggtttatagc gacatctatg atagagcgcc acaataacaa acaattgcgt   3860

tttattatta caaatccaat tttaaaaaaa gcggcagaac cggtcaaacc taaaagactg   3920

attacataaa tcttattcaa atttcaaaag tgccccaggg gctagtatct acgacacacc   3980

gagcggcgaa ctaataacgc tcactgaagg gaactccggt tccccgccgg cgcgcatggg   4040

tgagattcct tgaagttgag tattggccgt ccgctctacc gaaagttacg ggcaccattc   4100

aacccggtcc agcacggcgg ccgggtaacc gacttgctgc cccgagaatt atgcagcatt   4160

tttttggtgt atgtgggccc caaatgaagt gcaggtcaaa ccttgacagt gacgacaaat   4220

cgttgggcgg gtccagggcg aattttgcga caacatgtcg aggctcagca ggatgggccc   4280

aggtacagaa ttcgcggccg tacaacgcgt accggttaat taatctagag gcgcgccggg   4340

cccggccggc cagatcttga ttgtcgtttc ccgccttcag tttaaactat cagtgtttga   4400

caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata atcggatatt   4460

taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgt                     4503


<210>  11
<211>  442
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  11

Met Glu Asn Ala Lys Met Asn Ser Leu Ile Ala Gln Tyr Pro Leu Val 
1               5                   10                  15      


Lys Asp Leu Val Ala Leu Lys Glu Thr Thr Trp Phe Asn Pro Gly Thr 
            20                  25                  30          


Thr Ser Leu Ala Glu Gly Leu Pro Tyr Val Gly Leu Thr Glu Gln Asp 
        35                  40                  45              


Val Gln Asp Ala His Ala Arg Leu Ser Arg Phe Ala Pro Tyr Leu Ala 
    50                  55                  60                  


Lys Ala Phe Pro Glu Thr Ala Ala Thr Gly Gly Ile Ile Glu Ser Glu 
65                  70                  75                  80  


Leu Val Ala Ile Pro Ala Met Gln Lys Arg Leu Glu Lys Glu Tyr Gln 
                85                  90                  95      


Gln Pro Ile Ser Gly Gln Leu Leu Leu Lys Lys Asp Ser His Leu Pro 
            100                 105                 110         


Ile Ser Gly Ser Ile Lys Ala Arg Gly Gly Ile Tyr Glu Val Leu Ala 
        115                 120                 125             


His Ala Glu Lys Leu Ala Leu Glu Ala Gly Leu Leu Thr Leu Asp Asp 
    130                 135                 140                 


Asp Tyr Ser Lys Leu Leu Ser Pro Glu Phe Lys Gln Phe Phe Ser Gln 
145                 150                 155                 160 


Tyr Ser Ile Ala Val Gly Ser Thr Gly Asn Leu Gly Leu Ser Ile Gly 
                165                 170                 175     


Ile Met Ser Ala Arg Ile Gly Phe Lys Val Thr Val His Met Ser Ala 
            180                 185                 190         


Asp Ala Arg Ala Trp Lys Lys Ala Lys Leu Arg Ser His Gly Val Thr 
        195                 200                 205             


Val Val Glu Tyr Glu Gln Asp Tyr Gly Val Ala Val Glu Glu Gly Arg 
    210                 215                 220                 


Lys Ala Ala Gln Ser Asp Pro Asn Cys Phe Phe Ile Asp Asp Glu Asn 
225                 230                 235                 240 


Ser Arg Thr Leu Phe Leu Gly Tyr Ser Val Ala Gly Gln Arg Leu Lys 
                245                 250                 255     


Ala Gln Phe Ala Gln Gln Gly Arg Ile Val Asp Ala Asp Asn Pro Leu 
            260                 265                 270         


Phe Val Tyr Leu Pro Cys Gly Val Gly Gly Gly Pro Gly Gly Val Ala 
        275                 280                 285             


Phe Gly Leu Lys Leu Ala Phe Gly Asp His Val His Cys Phe Phe Ala 
    290                 295                 300                 


Glu Pro Thr His Ser Pro Cys Met Leu Leu Gly Val His Thr Gly Leu 
305                 310                 315                 320 


His Asp Gln Ile Ser Val Gln Asp Ile Gly Ile Asp Asn Leu Thr Ala 
                325                 330                 335     


Ala Asp Gly Leu Ala Val Gly Arg Ala Ser Gly Phe Val Gly Arg Ala 
            340                 345                 350         


Met Glu Arg Leu Leu Asp Gly Phe Tyr Thr Leu Ser Asp Gln Thr Met 
        355                 360                 365             


Tyr Asp Met Leu Gly Trp Leu Ala Gln Glu Glu Gly Ile Arg Leu Glu 
    370                 375                 380                 


Pro Ser Ala Leu Ala Gly Met Ala Gly Pro Gln Arg Val Cys Ala Ser 
385                 390                 395                 400 


Val Ser Tyr Gln Gln Met His Gly Phe Ser Ala Glu Gln Leu Arg Asn 
                405                 410                 415     


Thr Thr His Leu Val Trp Ala Thr Gly Gly Gly Met Val Pro Glu Glu 
            420                 425                 430         


Glu Met Asn Gln Tyr Leu Ala Lys Gly Arg 
        435                 440         


<210>  12
<211>  4254
<212>  DNA
<213>  Artificial

<220>
<223>  T-DNA region from RLM255, a RLM175-type binary vector containing 
       a p-ZmUBI+I::c-dao1::t-OCS selection cassette.


<220>
<221>  misc_structure
<222>  (1)..(128)
<223>  Left T-DNA border from pSB11

<220>
<221>  misc_binding
<222>  (47)..(71)
<223>  Left T-DNA border repeat from pSB11

<220>
<221>  promoter
<222>  (162)..(2149)
<223>  Zea mays ubiquitin promoter region with intron

<220>
<221>  Intron
<222>  (1144)..(2149)
<223>  Zea mays unbiquitin gene intron MubG1

<220>
<221>  CDS
<222>  (2192)..(3298)
<223>  Rhodosporidium toruloides D-amino acid oxidase CDS

<220>
<221>  terminator
<222>  (3327)..(4035)
<223>  Agrobacterium tumefaciens octapine synthase terminator region

<220>
<221>  misc_structure
<222>  (4110)..(4254)
<223>  Right T-DNA border from pSB11

<220>
<221>  misc_binding
<222>  (4150)..(4173)
<223>  Right T-DNA border repeat from pSB11

<400>  12
gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt     60

gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt    120

actgaattgg atccgcccgg gcggtaccaa gcttccgcgg ctgcagtgca gcgtgacccg    180

gtcgtgcccc tctctagaga taatgagcat tgcatgtcta agttataaaa aattaccaca    240

tatttttttt gtcacacttg tttgaagtgc agtttatcta tctttataca tatatttaaa    300

ctttactcta cgaataatat aatctatagt actacaataa tatcagtgtt ttagagaatc    360

atataaatga acagttagac atggtctaaa ggacaattga gtattttgac aacaggactc    420

tacagtttta tctttttagt gtgcatgtgt tctccttttt ttttgcaaat agcttcacct    480

atataatact tcatccattt tattagtaca tccatttagg gtttagggtt aatggttttt    540

atagactaat ttttttagta catctatttt attctatttt agcctctaaa ttaagaaaac    600

taaaactcta ttttagtttt tttatttaat agtttagata taaaatagaa taaaataaag    660

tgactaaaaa ttaaacaaat accctttaag aaattaaaaa aactaaggaa acatttttct    720

tgtttcgagt agataatgcc agcctgttaa acgccgtcga cgagtctaac ggacaccaac    780

cagcgaacca gcagcgtcgc gtcgggccaa gcgaagcaga cggcacggca tctctgtcgc    840

tgcctctgga cccctctcga gagttccgct ccaccgttgg acttgctccg ctgtcggcat    900

ccagaaattg cgtggcggag cggcagacgt gagccggcac ggcaggcggc ctcctcctcc    960

tctcacggca ccggcagcta cgggggattc ctttcccacc gctccttcgc tttcccttcc   1020

tcgcccgccg taataaatag acaccccctc cacaccctct ttccccaacc tcgtgttgtt   1080

cggagcgcac acacacacaa ccagatctcc cccaaatcca cccgtcggca cctccgcttc   1140

aaggtacgcc gctcgtcctc cccccccccc cccctctcta ccttctctag atcggcgttc   1200

cggtccatgg ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt   1260

tgtgttagat ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt   1320

ctgattgcta acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc   1380

gcagacggga tcgatttcat gatttttttt gtttcgttgc atagggtttg gtttgccctt   1440

ttcctttatt tcaatatatg ccgtgcactt gtttgtcggg tcatcttttc atgctttttt   1500

ttgtcttggt tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtagaattc   1560

tgtttcaaac tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat   1620

tcatagttac gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt   1680

gatgcgggtt ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg   1740

tggtgtggtt gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta   1800

cctggtgtat ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga   1860

gtttaagatg gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact   1920

gatgcatata catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta   1980

tctattataa taaacaagta tgttttataa ttatttcgat cttgatatac ttggatgatg   2040

gcatatgcag cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc   2100

ttggtactgt ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcagggtac   2160

ggatccggcg cgccactagt cccgggccac c atg cac tcg cag aag cgc gtc      2212
                                   Met His Ser Gln Lys Arg Val          
                                   1               5                    

gtt gtc ctc gga tca ggc gtt atc ggt ctg agc agc gcc ctc atc ctc     2260
Val Val Leu Gly Ser Gly Val Ile Gly Leu Ser Ser Ala Leu Ile Leu         
        10                  15                  20                      

gct cgg aag ggc tac agc gtg cat att ctc gcg cgc gac ttg ccg gag     2308
Ala Arg Lys Gly Tyr Ser Val His Ile Leu Ala Arg Asp Leu Pro Glu         
    25                  30                  35                          

gac gtc tcg agc cag act ttc gct tca cca tgg gct ggc gcg aat tgg     2356
Asp Val Ser Ser Gln Thr Phe Ala Ser Pro Trp Ala Gly Ala Asn Trp         
40                  45                  50                  55          

acg cct ttc atg acg ctt aca gac ggt cct cga caa gca aaa tgg gaa     2404
Thr Pro Phe Met Thr Leu Thr Asp Gly Pro Arg Gln Ala Lys Trp Glu         
                60                  65                  70              

gaa tcg act ttc aag aag tgg gtc gag ttg gtc ccg acg ggc cat gcc     2452
Glu Ser Thr Phe Lys Lys Trp Val Glu Leu Val Pro Thr Gly His Ala         
            75                  80                  85                  

atg tgg ctc aag ggg acg agg cgg ttc gcg cag aac gaa gac ggc ttg     2500
Met Trp Leu Lys Gly Thr Arg Arg Phe Ala Gln Asn Glu Asp Gly Leu         
        90                  95                  100                     

ctc ggg cac tgg tac aag gac atc acg cca aat tac cgc ccc ctc cca     2548
Leu Gly His Trp Tyr Lys Asp Ile Thr Pro Asn Tyr Arg Pro Leu Pro         
    105                 110                 115                         

tct tcc gaa tgt cca cct ggc gct atc ggc gta acc tac gac acc ctc     2596
Ser Ser Glu Cys Pro Pro Gly Ala Ile Gly Val Thr Tyr Asp Thr Leu         
120                 125                 130                 135         

tcc gtc cac gca cca aag tac tgc cag tac ctt gca aga gag ctg cag     2644
Ser Val His Ala Pro Lys Tyr Cys Gln Tyr Leu Ala Arg Glu Leu Gln         
                140                 145                 150             

aag ctc ggc gcg acg ttt gag aga cgg acc gtt acg tcg ctt gag cag     2692
Lys Leu Gly Ala Thr Phe Glu Arg Arg Thr Val Thr Ser Leu Glu Gln         
            155                 160                 165                 

gcg ttc gac ggt gcg gat ttg gtg gtc aac gct acg gga ctt ggc gcc     2740
Ala Phe Asp Gly Ala Asp Leu Val Val Asn Ala Thr Gly Leu Gly Ala         
        170                 175                 180                     

aag tcg att gcg ggc atc gac gac caa gcc gcc gag cca atc cgc ggg     2788
Lys Ser Ile Ala Gly Ile Asp Asp Gln Ala Ala Glu Pro Ile Arg Gly         
    185                 190                 195                         

caa acc gtc ctc gtc aag tcc cca tgc aag cga tgc acg atg gac tcg     2836
Gln Thr Val Leu Val Lys Ser Pro Cys Lys Arg Cys Thr Met Asp Ser         
200                 205                 210                 215         

tcc gac ccc gct tct ccc gcc tac atc att ccc cga cca ggt ggc gaa     2884
Ser Asp Pro Ala Ser Pro Ala Tyr Ile Ile Pro Arg Pro Gly Gly Glu         
                220                 225                 230             

gtc atc tgc ggc ggg acg tac ggc gtg gga gac tgg gac ttg tct gtc     2932
Val Ile Cys Gly Gly Thr Tyr Gly Val Gly Asp Trp Asp Leu Ser Val         
            235                 240                 245                 

aac cca gag acg gtc cag cgg atc ctc aag cac tgc ttg cgc ctc gac     2980
Asn Pro Glu Thr Val Gln Arg Ile Leu Lys His Cys Leu Arg Leu Asp         
        250                 255                 260                     

ccg acc atc tcg agc gac gga acg atc gaa ggc atc gag gtc ctc cgc     3028
Pro Thr Ile Ser Ser Asp Gly Thr Ile Glu Gly Ile Glu Val Leu Arg         
    265                 270                 275                         

cac aac gtc ggc ttg cga cct gca cga cga ggc gga ccc cgc gtt gag     3076
His Asn Val Gly Leu Arg Pro Ala Arg Arg Gly Gly Pro Arg Val Glu         
280                 285                 290                 295         

gca gaa cgg atc gtc ctg cct ctc gac cgg aca aag tcg ccc ctc tcg     3124
Ala Glu Arg Ile Val Leu Pro Leu Asp Arg Thr Lys Ser Pro Leu Ser         
                300                 305                 310             

ctc ggc agg ggc agc gca cga gcg gcg aag gag aag gag gtc acg ctt     3172
Leu Gly Arg Gly Ser Ala Arg Ala Ala Lys Glu Lys Glu Val Thr Leu         
            315                 320                 325                 

gtg cat gcg tat ggc ttc tcg agt gcg gga tac cag cag agt tgg ggc     3220
Val His Ala Tyr Gly Phe Ser Ser Ala Gly Tyr Gln Gln Ser Trp Gly         
        330                 335                 340                     

gcg gcg gag gat gtc gcg cag ctc gtc gac gag gcg ttc cag cgg tac     3268
Ala Ala Glu Asp Val Ala Gln Leu Val Asp Glu Ala Phe Gln Arg Tyr         
    345                 350                 355                         

cac ggc gcg gcg cgg gag tcg aag ttg tag ggcgggattc ctgcaggagc       3318
His Gly Ala Ala Arg Glu Ser Lys Leu                                     
360                 365                                                 

tcaatcgatc ctgctttaat gagatatgcg agacgcctat gatcgcatga tatttgcttt   3378

caattctgtt gtgcacgttg taaaaaacct gagcatgtgt agctcagatc cttaccgccg   3438

gtttcggttc attctaatga atatatcacc cgttactatc gtatttttat gaataatatt   3498

ctccgttcaa tttactgatt gtaccctact acttatatgt acaatattaa aatgaaaaca   3558

atatattgtg ctgaataggt ttatagcgac atctatgata gagcgccaca ataacaaaca   3618

attgcgtttt attattacaa atccaatttt aaaaaaagcg gcagaaccgg tcaaacctaa   3678

aagactgatt acataaatct tattcaaatt tcaaaagtgc cccaggggct agtatctacg   3738

acacaccgag cggcgaacta ataacgctca ctgaagggaa ctccggttcc ccgccggcgc   3798

gcatgggtga gattccttga agttgagtat tggccgtccg ctctaccgaa agttacgggc   3858

accattcaac ccggtccagc acggcggccg ggtaaccgac ttgctgcccc gagaattatg   3918

cagcattttt ttggtgtatg tgggccccaa atgaagtgca ggtcaaacct tgacagtgac   3978

gacaaatcgt tgggcgggtc cagggcgaat tttgcgacaa catgtcgagg ctcagcagga   4038

tgggcccagg tacagaattc gcggccgctt aattaatcta gaggcgcgcc gggcccggcc   4098

ggccagatct tgattgtcgt ttcccgcctt cagtttaaac tatcagtgtt tgacaggata   4158

tattggcggg taaacctaag agaaaagagc gtttattaga ataatcggat atttaaaagg   4218

gcgtgaaaag gtttatccgt tcgtccattt gtatgt                             4254


<210>  13
<211>  368
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  13

Met His Ser Gln Lys Arg Val Val Val Leu Gly Ser Gly Val Ile Gly 
1               5                   10                  15      


Leu Ser Ser Ala Leu Ile Leu Ala Arg Lys Gly Tyr Ser Val His Ile 
            20                  25                  30          


Leu Ala Arg Asp Leu Pro Glu Asp Val Ser Ser Gln Thr Phe Ala Ser 
        35                  40                  45              


Pro Trp Ala Gly Ala Asn Trp Thr Pro Phe Met Thr Leu Thr Asp Gly 
    50                  55                  60                  


Pro Arg Gln Ala Lys Trp Glu Glu Ser Thr Phe Lys Lys Trp Val Glu 
65                  70                  75                  80  


Leu Val Pro Thr Gly His Ala Met Trp Leu Lys Gly Thr Arg Arg Phe 
                85                  90                  95      


Ala Gln Asn Glu Asp Gly Leu Leu Gly His Trp Tyr Lys Asp Ile Thr 
            100                 105                 110         


Pro Asn Tyr Arg Pro Leu Pro Ser Ser Glu Cys Pro Pro Gly Ala Ile 
        115                 120                 125             


Gly Val Thr Tyr Asp Thr Leu Ser Val His Ala Pro Lys Tyr Cys Gln 
    130                 135                 140                 


Tyr Leu Ala Arg Glu Leu Gln Lys Leu Gly Ala Thr Phe Glu Arg Arg 
145                 150                 155                 160 


Thr Val Thr Ser Leu Glu Gln Ala Phe Asp Gly Ala Asp Leu Val Val 
                165                 170                 175     


Asn Ala Thr Gly Leu Gly Ala Lys Ser Ile Ala Gly Ile Asp Asp Gln 
            180                 185                 190         


Ala Ala Glu Pro Ile Arg Gly Gln Thr Val Leu Val Lys Ser Pro Cys 
        195                 200                 205             


Lys Arg Cys Thr Met Asp Ser Ser Asp Pro Ala Ser Pro Ala Tyr Ile 
    210                 215                 220                 


Ile Pro Arg Pro Gly Gly Glu Val Ile Cys Gly Gly Thr Tyr Gly Val 
225                 230                 235                 240 


Gly Asp Trp Asp Leu Ser Val Asn Pro Glu Thr Val Gln Arg Ile Leu 
                245                 250                 255     


Lys His Cys Leu Arg Leu Asp Pro Thr Ile Ser Ser Asp Gly Thr Ile 
            260                 265                 270         


Glu Gly Ile Glu Val Leu Arg His Asn Val Gly Leu Arg Pro Ala Arg 
        275                 280                 285             


Arg Gly Gly Pro Arg Val Glu Ala Glu Arg Ile Val Leu Pro Leu Asp 
    290                 295                 300                 


Arg Thr Lys Ser Pro Leu Ser Leu Gly Arg Gly Ser Ala Arg Ala Ala 
305                 310                 315                 320 


Lys Glu Lys Glu Val Thr Leu Val His Ala Tyr Gly Phe Ser Ser Ala 
                325                 330                 335     


Gly Tyr Gln Gln Ser Trp Gly Ala Ala Glu Asp Val Ala Gln Leu Val 
            340                 345                 350         


Asp Glu Ala Phe Gln Arg Tyr His Gly Ala Ala Arg Glu Ser Lys Leu 
        355                 360                 365             


<210>  14
<211>  5035
<212>  DNA
<213>  Artificial

<220>
<223>  RLM220 binary vector derived from pSB11


<220>
<221>  misc_structure
<222>  (5)..(132)
<223>  Left T-DNA border from pSB11

<220>
<221>  misc_binding
<222>  (51)..(75)
<223>  Left T-DNA border repeat from pSB11

<220>
<221>  misc_structure
<222>  (232)..(376)
<223>  Right T-DNA border from pSB11

<220>
<221>  misc_binding
<222>  (272)..(295)
<223>  Right T-DNA border repeat from pSB11

<220>
<221>  misc_structure
<222>  (777)..(3511)
<223>  pSB1 recombination target

<220>
<221>  rep_origin
<222>  (2654)..(2934)
<223>  ColEI replication origin from pSB11

<220>
<221>  misc_structure
<222>  (3074)..(3334)
<223>  ColEI BOM site from pSB11

<220>
<221>  misc_feature
<222>  (3793)..(4608)
<223>  cds encoding Kanamycin Resistance selection gene from Tn903

<400>  14
agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg tggcaggata     60

tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg gacgttttta    120

atgtactgaa ttggatccgc ccgggcggta ccgaattcgc ggccgcaagc ttgtacaacg    180

cgtaccggtt aattaatcta gaggcgcgcc gggcccggcc ggccagatct tgattgtcgt    240

ttcccgcctt cagtttaaac tatcagtgtt tgacaggata tattggcggg taaacctaag    300

agaaaagagc gtttattaga ataatcggat atttaaaagg gcgtgaaaag gtttatccgt    360

tcgtccattt gtatgtgcat gccaaccaca gggttcccct cgggagtgct tggcattccg    420

tgcgataatg acttctgttc aaccacccaa acgtcggaaa gcctgacgac ggagcagcat    480

tccaaaaaga tcccttggct cgtctgggtc ggctagaagg tcgagtgggc tgctgtggct    540

tgatccctca acgcggtcgc ggacgtagcg cagcgccgaa aaatcctcga tcgcaaatcc    600

gacgctgtcg aaaagcgtga tctgcttgtc gctctttcgg ccgacgtcct ggccagtcat    660

cacgcgccaa agttccgtca caggatgatc tggcgcgagt tgctggatct cgccttcaat    720

ccgggtctgt ggcgggaact ccacgaaaat atccgaacgc agcaagatcg tcgaccaatt    780

cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa    840

tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt    900

tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc    960

ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc   1020

ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa   1080

aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg   1140

gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag   1200

ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc   1260

gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta   1320

cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg   1380

cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca   1440

acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac   1500

caaacgacga gcgtgacacc acgatgccgg gggggggggg gggggacatg aggttgcccc   1560

gtattcagtg tcgctgattt gtattgtctg aagttgtttt tacgttaagt tgatgcagat   1620

caattaatac gatacctgcg tcataattga ttatttgacg tggtttgatg gcctccacgc   1680

acgttgtgat atgtagatga taatcattat cactttacgg gtcctttccg gtgatccgac   1740

aggttacggg gcggcgacct cgcgggtttt cgctatttat gaaaattttc cggtttaagg   1800

cgtttccgtt cttcttcgtc ataacttaat gtttttattt aaaataccct ctgaaaagaa   1860

aggaaacgac aggtgctgaa agcgagcttt ttggcctctg tcgtttcctt tctctgtttt   1920

tgtccgtgga atgaacaatg gaaccccccc cccccccccc tgcagcaatg gcaacaacgt   1980

tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact   2040

ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt   2100

ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg   2160

ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta   2220

tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac   2280

tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta   2340

aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt   2400

tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt   2460

tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt   2520

gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc   2580

agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg   2640

tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg   2700

ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt   2760

cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac   2820

tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg   2880

acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg   2940

gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat   3000

ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt   3060

tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg   3120

attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa   3180

cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc   3240

tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct   3300

ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc   3360

tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc   3420

atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc   3480

gtcatcaccg aaacgcgcga ggcagcagat cccccgatca agtagataca ctacatatat   3540

ctacaataga catcgagccg gaaggtgatg tttactttcc tgaaatcccc agcaatttta   3600

ggccagtttt tacccaagac ttcgcctcta acataaatta tagttaccaa atctggcaaa   3660

agggttgacc gggggggggg ggaaagccac gttgtgtctc aaaatctctg atgttacatt   3720

gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat   3780

acaaggggtg ttatgagcca tattcaacgg gaaacgtctt gctcgaggcc gcgattaaat   3840

tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca   3900

ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat   3960

ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg   4020

gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta   4080

ctcaccactg cgatccccgg gaaaacagca ttccaggtat tagaagaata tcctgattca   4140

ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt   4200

tgtaattgtc cttttaacag cgaccgcgta tttcgtctcg ctcaggcgca atcacgaatg   4260

aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa   4320

caagtctgga aagaaatgca taagcttttg ccattctcac cggattcagt cgtcactcat   4380

ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat   4440

gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc   4500

ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct   4560

gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt   4620

aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgg ctttgttgaa   4680

taaatcgaac ttttgctgag ttgaaggatc agatcacgca tcttcccgac aacgcagacc   4740

gttccgtggc aaagcaaaag ttcaaaatca ccaactggtc cacctacaac aaagctctca   4800

tcaaccgtgg ctccctcact ttctggctgg atgatggggc gattcaggga tcacaggcag   4860

caacgctctg tcatcgttac aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa   4920

acccggcagc ttagttgccg ttcttccgaa tagcatcggt aacatgagca aagtctgccg   4980

ccttacaacg gctctcccgc tgacgccgtc ccggactgat gggctgcctg tatcg        5035


<210>  15
<211>  3160
<212>  DNA
<213>  Artificial

<220>
<223>  T-DNA region from RLM242 a RLM220-type binary vector containing a
       p-ScBV::c-dao1::t-NOS selection cassette.


<220>
<221>  misc_structure
<222>  (1)..(128)
<223>  Left T-DNA border from pSB11

<220>
<221>  misc_binding
<222>  (47)..(71)
<223>  Left T-DNA border repeat from pSB11

<220>
<221>  promoter
<222>  (154)..(1551)
<223>  Sugarcane bacilliform virus [ScBV] promoter region

<220>
<221>  CDS
<222>  (1572)..(2678)
<223>  Rhodosporidium toruloides D-amino acid oxidase CDS

<220>
<221>  terminator
<222>  (2700)..(2966)
<223>  Agrobacterium tumefaciens nopaline synthase terminator region

<220>
<221>  misc_structure
<222>  (3016)..(3160)
<223>  Right T-DNA border from pSB11

<220>
<221>  misc_binding
<222>  (3056)..(3079)
<223>  Right T-DNA border repeat from pSB11

<400>  15
gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt     60

gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt    120

actgaattgg atccgcccgg gcggtacatc ttaaatcctg gctagcaaca ctgaactatg    180

ccagaaacca catcaaagat atgggcaagc ttcttggccc attatatcca aagacctcag    240

agaaaggtga gcgaaggctc aattcagaag attggaagct gatcaatagg atcaagacaa    300

tggtgagaac gcttccaaat ctcactattc caccagaaga tgcatacatt atcattgaaa    360

cagatgcatg tgcaactgga tggggagcag tatgcaagtg gaagaaaaac aaggcagacc    420

caagaaatac agagcaaatc tgtaggtatg ccagtggaaa atttgataag ccaaaaggaa    480

cctgtgatgc agaaatctat ggggttatga atggcttaga aaagatgaga ttgttctact    540

tggacaaaag agagatcaca gtcagaactg acagtagtgc aatcgaaagg ttctacaaca    600

agagtgctga acacaagcct tctgagatca gatggatcag gttcatggac tacatcactg    660

gtgcaggacc agagatagtc attgaacaca taaaagggaa gagcaatggt ttagctgaca    720

tcttgtccag gctcaaagcc aaattagctc agaatgaacc aacggaagag atgatcctgc    780

ttacacaagc cataagggaa gtaattcctt atccagatca tccatacact gagcaactca    840

gagaatgggg aaacaaaatt ctggatccat tccccacatt caagaaggac atgttcgaaa    900

gaacagagca agcttttatg ctaacagagg aaccagttct actctgtgca tgcaggaagc    960

ctgcaattca gttagtgtcc agaacatctg ccaacccagg aaggaaattc ttcaagtgcg   1020

caatgaacaa atgccattgc tggtactggg cagatctcat tgaagaacac attcaagaca   1080

gaattgatga atttctcaag aatcttgaag ttctgaagac cggtggcgtg caaacaatgg   1140

aggaggaact tatgaaggaa gtcaccaagc tgaagataga agagcaggag ttcgaggaat   1200

accaggccac accaagggct atgtcgccag tagccgcaga agatgtgcta gatctccaag   1260

acgtaagcaa tgacgattga ggaggcattg acgtcaggga tgaccgcagc ggagagtact   1320

gggcccattc agtggatgct ccactgagtt gtattattgt gtgcttttcg gacaagtgtg   1380

ctgtccactt tcttttggca cctgtgccac tttattcctt gtctgccacg atgcctttgc   1440

ttagcttgta agcaaggatc gcagtgcgtg tgtgacacca ccccccttcc gacgctctgc   1500

ctatataagg caccgtctgt aagctcttac gatcatcggt agttcaccaa gctcgaggat   1560

cccgggccac c atg cac tcg cag aag cgc gtc gtt gtc ctc gga tca ggc    1610
             Met His Ser Gln Lys Arg Val Val Val Leu Gly Ser Gly        
             1               5                   10                     

gtt atc ggt ctg agc agc gcc ctc atc ctc gct cgg aag ggc tac agc     1658
Val Ile Gly Leu Ser Ser Ala Leu Ile Leu Ala Arg Lys Gly Tyr Ser         
    15                  20                  25                          

gtg cat att ctc gcg cgc gac ttg ccg gag gac gtc tcg agc cag act     1706
Val His Ile Leu Ala Arg Asp Leu Pro Glu Asp Val Ser Ser Gln Thr         
30                  35                  40                  45          

ttc gct tca cca tgg gct ggc gcg aat tgg acg cct ttc atg acg ctt     1754
Phe Ala Ser Pro Trp Ala Gly Ala Asn Trp Thr Pro Phe Met Thr Leu         
                50                  55                  60              

aca gac ggt cct cga caa gca aaa tgg gaa gaa tcg act ttc aag aag     1802
Thr Asp Gly Pro Arg Gln Ala Lys Trp Glu Glu Ser Thr Phe Lys Lys         
            65                  70                  75                  

tgg gtc gag ttg gtc ccg acg ggc cat gcc atg tgg ctc aag ggg acg     1850
Trp Val Glu Leu Val Pro Thr Gly His Ala Met Trp Leu Lys Gly Thr         
        80                  85                  90                      

agg cgg ttc gcg cag aac gaa gac ggc ttg ctc ggg cac tgg tac aag     1898
Arg Arg Phe Ala Gln Asn Glu Asp Gly Leu Leu Gly His Trp Tyr Lys         
    95                  100                 105                         

gac atc acg cca aat tac cgc ccc ctc cca tct tcc gaa tgt cca cct     1946
Asp Ile Thr Pro Asn Tyr Arg Pro Leu Pro Ser Ser Glu Cys Pro Pro         
110                 115                 120                 125         

ggc gct atc ggc gta acc tac gac acc ctc tcc gtc cac gca cca aag     1994
Gly Ala Ile Gly Val Thr Tyr Asp Thr Leu Ser Val His Ala Pro Lys         
                130                 135                 140             

tac tgc cag tac ctt gca aga gag ctg cag aag ctc ggc gcg acg ttt     2042
Tyr Cys Gln Tyr Leu Ala Arg Glu Leu Gln Lys Leu Gly Ala Thr Phe         
            145                 150                 155                 

gag aga cgg acc gtt acg tcg ctt gag cag gcg ttc gac ggt gcg gat     2090
Glu Arg Arg Thr Val Thr Ser Leu Glu Gln Ala Phe Asp Gly Ala Asp         
        160                 165                 170                     

ttg gtg gtc aac gct acg gga ctt ggc gcc aag tcg att gcg ggc atc     2138
Leu Val Val Asn Ala Thr Gly Leu Gly Ala Lys Ser Ile Ala Gly Ile         
    175                 180                 185                         

gac gac caa gcc gcc gag cca atc cgc ggg caa acc gtc ctc gtc aag     2186
Asp Asp Gln Ala Ala Glu Pro Ile Arg Gly Gln Thr Val Leu Val Lys         
190                 195                 200                 205         

tcc cca tgc aag cga tgc acg atg gac tcg tcc gac ccc gct tct ccc     2234
Ser Pro Cys Lys Arg Cys Thr Met Asp Ser Ser Asp Pro Ala Ser Pro         
                210                 215                 220             

gcc tac atc att ccc cga cca ggt ggc gaa gtc atc tgc ggc ggg acg     2282
Ala Tyr Ile Ile Pro Arg Pro Gly Gly Glu Val Ile Cys Gly Gly Thr         
            225                 230                 235                 

tac ggc gtg gga gac tgg gac ttg tct gtc aac cca gag acg gtc cag     2330
Tyr Gly Val Gly Asp Trp Asp Leu Ser Val Asn Pro Glu Thr Val Gln         
        240                 245                 250                     

cgg atc ctc aag cac tgc ttg cgc ctc gac ccg acc atc tcg agc gac     2378
Arg Ile Leu Lys His Cys Leu Arg Leu Asp Pro Thr Ile Ser Ser Asp         
    255                 260                 265                         

gga acg atc gaa ggc atc gag gtc ctc cgc cac aac gtc ggc ttg cga     2426
Gly Thr Ile Glu Gly Ile Glu Val Leu Arg His Asn Val Gly Leu Arg         
270                 275                 280                 285         

cct gca cga cga ggc gga ccc cgc gtt gag gca gaa cgg atc gtc ctg     2474
Pro Ala Arg Arg Gly Gly Pro Arg Val Glu Ala Glu Arg Ile Val Leu         
                290                 295                 300             

cct ctc gac cgg aca aag tcg ccc ctc tcg ctc ggc agg ggc agc gca     2522
Pro Leu Asp Arg Thr Lys Ser Pro Leu Ser Leu Gly Arg Gly Ser Ala         
            305                 310                 315                 

cga gcg gcg aag gag aag gag gtc acg ctt gtg cat gcg tat ggc ttc     2570
Arg Ala Ala Lys Glu Lys Glu Val Thr Leu Val His Ala Tyr Gly Phe         
        320                 325                 330                     

tcg agt gcg gga tac cag cag agt tgg ggc gcg gcg gag gat gtc gcg     2618
Ser Ser Ala Gly Tyr Gln Gln Ser Trp Gly Ala Ala Glu Asp Val Ala         
    335                 340                 345                         

cag ctc gtc gac gag gcg ttc cag cgg tac cac ggc gcg gcg cgg gag     2666
Gln Leu Val Asp Glu Ala Phe Gln Arg Tyr His Gly Ala Ala Arg Glu         
350                 355                 360                 365         

tcg aag ttg tag ggcgggattc ctgcaggagc tcgaatttcc ccgatcgttc         2718
Ser Lys Leu                                                             
                                                                        

aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat   2778

catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt   2838

atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga   2898

aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact   2958

agatcgggaa ttccttaatt aatctagagg cgcgccgggc ccggccggcc agatcttgat   3018

tgtcgtttcc cgccttcagt ttaaactatc agtgtttgac aggatatatt ggcgggtaaa   3078

cctaagagaa aagagcgttt attagaataa tcggatattt aaaagggcgt gaaaaggttt   3138

atccgttcgt ccatttgtat gt                                            3160


<210>  16
<211>  368
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  16

Met His Ser Gln Lys Arg Val Val Val Leu Gly Ser Gly Val Ile Gly 
1               5                   10                  15      


Leu Ser Ser Ala Leu Ile Leu Ala Arg Lys Gly Tyr Ser Val His Ile 
            20                  25                  30          


Leu Ala Arg Asp Leu Pro Glu Asp Val Ser Ser Gln Thr Phe Ala Ser 
        35                  40                  45              


Pro Trp Ala Gly Ala Asn Trp Thr Pro Phe Met Thr Leu Thr Asp Gly 
    50                  55                  60                  


Pro Arg Gln Ala Lys Trp Glu Glu Ser Thr Phe Lys Lys Trp Val Glu 
65                  70                  75                  80  


Leu Val Pro Thr Gly His Ala Met Trp Leu Lys Gly Thr Arg Arg Phe 
                85                  90                  95      


Ala Gln Asn Glu Asp Gly Leu Leu Gly His Trp Tyr Lys Asp Ile Thr 
            100                 105                 110         


Pro Asn Tyr Arg Pro Leu Pro Ser Ser Glu Cys Pro Pro Gly Ala Ile 
        115                 120                 125             


Gly Val Thr Tyr Asp Thr Leu Ser Val His Ala Pro Lys Tyr Cys Gln 
    130                 135                 140                 


Tyr Leu Ala Arg Glu Leu Gln Lys Leu Gly Ala Thr Phe Glu Arg Arg 
145                 150                 155                 160 


Thr Val Thr Ser Leu Glu Gln Ala Phe Asp Gly Ala Asp Leu Val Val 
                165                 170                 175     


Asn Ala Thr Gly Leu Gly Ala Lys Ser Ile Ala Gly Ile Asp Asp Gln 
            180                 185                 190         


Ala Ala Glu Pro Ile Arg Gly Gln Thr Val Leu Val Lys Ser Pro Cys 
        195                 200                 205             


Lys Arg Cys Thr Met Asp Ser Ser Asp Pro Ala Ser Pro Ala Tyr Ile 
    210                 215                 220                 


Ile Pro Arg Pro Gly Gly Glu Val Ile Cys Gly Gly Thr Tyr Gly Val 
225                 230                 235                 240 


Gly Asp Trp Asp Leu Ser Val Asn Pro Glu Thr Val Gln Arg Ile Leu 
                245                 250                 255     


Lys His Cys Leu Arg Leu Asp Pro Thr Ile Ser Ser Asp Gly Thr Ile 
            260                 265                 270         


Glu Gly Ile Glu Val Leu Arg His Asn Val Gly Leu Arg Pro Ala Arg 
        275                 280                 285             


Arg Gly Gly Pro Arg Val Glu Ala Glu Arg Ile Val Leu Pro Leu Asp 
    290                 295                 300                 


Arg Thr Lys Ser Pro Leu Ser Leu Gly Arg Gly Ser Ala Arg Ala Ala 
305                 310                 315                 320 


Lys Glu Lys Glu Val Thr Leu Val His Ala Tyr Gly Phe Ser Ser Ala 
                325                 330                 335     


Gly Tyr Gln Gln Ser Trp Gly Ala Ala Glu Asp Val Ala Gln Leu Val 
            340                 345                 350         


Asp Glu Ala Phe Gln Arg Tyr His Gly Ala Ala Arg Glu Ser Lys Leu 
        355                 360                 365             


<210>  17
<211>  2560
<212>  DNA
<213>  Artificial

<220>
<223>  T-DNA region from RLM241, a RLM220-type binary vector containing 
       a p-ZmAHASL::c-dao1::t-NOS selection cassette.


<220>
<221>  misc_structure
<222>  (1)..(128)
<223>  Left T-DNA border from pSB11

<220>
<221>  misc_binding
<222>  (47)..(71)
<223>  Left T-DNA border repeat from pSB11

<220>
<221>  promoter
<222>  (153)..(952)
<223>  AHASL2 [XI12] promoter and 5'UTR regions from Z. mays 
       acetohydroxyacid synthase (AHAS108) gene

<220>
<221>  CDS
<222>  (972)..(2078)
<223>  Rhodosporidium toruloides D-amino acid oxidase CDS

<220>
<221>  terminator
<222>  (2111)..(2363)
<223>  Agrobacterium tumefaciens nopaline synthase terminator region

<220>
<221>  misc_structure
<222>  (2416)..(2560)
<223>  Right T-DNA Border from pSB11

<220>
<221>  misc_binding
<222>  (2456)..(2479)
<223>  Right T-DNA border repeat from pSB11

<400>  17
gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt     60

gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt    120

actgaattgg atccgcccgg gcggtacatg caggtcaacg gatcacctat caacatccca    180

gctaaaaaca gtaaaaaggg ggaaaacgtg ggtgagttga gtctgtcttg tggaaaaaac    240

gttttagttt ctcctggaat taacaataaa aacagttgaa caagattgac tgttcctccg    300

ggagggtttg gaacatcgtt acagatgtga gcgaaaggtg aggaaacaga gcggagggct    360

tggaggtgac ctcggtagtc gacgccggag ttgagcctga tgacgacacc gtactggcgt    420

accaggccta gtagtgaaca ccgggcctga agctgtcgcc gccgctgctc atcttgtggg    480

ctgtgcccgg tgtccctgtt gcggattgcg ggtggcagcc tggcaggtgg gtgcgacccg    540

tttggactcc ctgatctggg ccctttgtgt cagtaccgtc tgtactccga tgacatgcac    600

accgtcgtcc acagtcaagt ccacaatctc ccctcttttt ttaacggaat agttcaaaat    660

ctccttgacg cacgctatcg tgtaccagcg ctcactggac accacgtttg taatccacgc    720

cgacacgtcg gtcccacgtc gacaggcccc accgtccggt ctgtagcgtg tacgtattcg    780

ggcgacggac gtgtcgtcgt cgtcttgcga gtcccattcc catcaccatc tgagccacac    840

atcctctgaa caaaagcagg gaggcctcta cgcacatccc cctttctccc actccgtgtc    900

cgtggcaccc accccaaacc ctcgcgccgc ctccgagaca gccgccgcaa cctcgaggat    960

cccgggccac c atg cac tcg cag aag cgc gtc gtt gtc ctc gga tca ggc    1010
             Met His Ser Gln Lys Arg Val Val Val Leu Gly Ser Gly        
             1               5                   10                     

gtt atc ggt ctg agc agc gcc ctc atc ctc gct cgg aag ggc tac agc     1058
Val Ile Gly Leu Ser Ser Ala Leu Ile Leu Ala Arg Lys Gly Tyr Ser         
    15                  20                  25                          

gtg cat att ctc gcg cgc gac ttg ccg gag gac gtc tcg agc cag act     1106
Val His Ile Leu Ala Arg Asp Leu Pro Glu Asp Val Ser Ser Gln Thr         
30                  35                  40                  45          

ttc gct tca cca tgg gct ggc gcg aat tgg acg cct ttc atg acg ctt     1154
Phe Ala Ser Pro Trp Ala Gly Ala Asn Trp Thr Pro Phe Met Thr Leu         
                50                  55                  60              

aca gac ggt cct cga caa gca aaa tgg gaa gaa tcg act ttc aag aag     1202
Thr Asp Gly Pro Arg Gln Ala Lys Trp Glu Glu Ser Thr Phe Lys Lys         
            65                  70                  75                  

tgg gtc gag ttg gtc ccg acg ggc cat gcc atg tgg ctc aag ggg acg     1250
Trp Val Glu Leu Val Pro Thr Gly His Ala Met Trp Leu Lys Gly Thr         
        80                  85                  90                      

agg cgg ttc gcg cag aac gaa gac ggc ttg ctc ggg cac tgg tac aag     1298
Arg Arg Phe Ala Gln Asn Glu Asp Gly Leu Leu Gly His Trp Tyr Lys         
    95                  100                 105                         

gac atc acg cca aat tac cgc ccc ctc cca tct tcc gaa tgt cca cct     1346
Asp Ile Thr Pro Asn Tyr Arg Pro Leu Pro Ser Ser Glu Cys Pro Pro         
110                 115                 120                 125         

ggc gct atc ggc gta acc tac gac acc ctc tcc gtc cac gca cca aag     1394
Gly Ala Ile Gly Val Thr Tyr Asp Thr Leu Ser Val His Ala Pro Lys         
                130                 135                 140             

tac tgc cag tac ctt gca aga gag ctg cag aag ctc ggc gcg acg ttt     1442
Tyr Cys Gln Tyr Leu Ala Arg Glu Leu Gln Lys Leu Gly Ala Thr Phe         
            145                 150                 155                 

gag aga cgg acc gtt acg tcg ctt gag cag gcg ttc gac ggt gcg gat     1490
Glu Arg Arg Thr Val Thr Ser Leu Glu Gln Ala Phe Asp Gly Ala Asp         
        160                 165                 170                     

ttg gtg gtc aac gct acg gga ctt ggc gcc aag tcg att gcg ggc atc     1538
Leu Val Val Asn Ala Thr Gly Leu Gly Ala Lys Ser Ile Ala Gly Ile         
    175                 180                 185                         

gac gac caa gcc gcc gag cca atc cgc ggg caa acc gtc ctc gtc aag     1586
Asp Asp Gln Ala Ala Glu Pro Ile Arg Gly Gln Thr Val Leu Val Lys         
190                 195                 200                 205         

tcc cca tgc aag cga tgc acg atg gac tcg tcc gac ccc gct tct ccc     1634
Ser Pro Cys Lys Arg Cys Thr Met Asp Ser Ser Asp Pro Ala Ser Pro         
                210                 215                 220             

gcc tac atc att ccc cga cca ggt ggc gaa gtc atc tgc ggc ggg acg     1682
Ala Tyr Ile Ile Pro Arg Pro Gly Gly Glu Val Ile Cys Gly Gly Thr         
            225                 230                 235                 

tac ggc gtg gga gac tgg gac ttg tct gtc aac cca gag acg gtc cag     1730
Tyr Gly Val Gly Asp Trp Asp Leu Ser Val Asn Pro Glu Thr Val Gln         
        240                 245                 250                     

cgg atc ctc aag cac tgc ttg cgc ctc gac ccg acc atc tcg agc gac     1778
Arg Ile Leu Lys His Cys Leu Arg Leu Asp Pro Thr Ile Ser Ser Asp         
    255                 260                 265                         

gga acg atc gaa ggc atc gag gtc ctc cgc cac aac gtc ggc ttg cga     1826
Gly Thr Ile Glu Gly Ile Glu Val Leu Arg His Asn Val Gly Leu Arg         
270                 275                 280                 285         

cct gca cga cga ggc gga ccc cgc gtt gag gca gaa cgg atc gtc ctg     1874
Pro Ala Arg Arg Gly Gly Pro Arg Val Glu Ala Glu Arg Ile Val Leu         
                290                 295                 300             

cct ctc gac cgg aca aag tcg ccc ctc tcg ctc ggc agg ggc agc gca     1922
Pro Leu Asp Arg Thr Lys Ser Pro Leu Ser Leu Gly Arg Gly Ser Ala         
            305                 310                 315                 

cga gcg gcg aag gag aag gag gtc acg ctt gtg cat gcg tat ggc ttc     1970
Arg Ala Ala Lys Glu Lys Glu Val Thr Leu Val His Ala Tyr Gly Phe         
        320                 325                 330                     

tcg agt gcg gga tac cag cag agt tgg ggc gcg gcg gag gat gtc gcg     2018
Ser Ser Ala Gly Tyr Gln Gln Ser Trp Gly Ala Ala Glu Asp Val Ala         
    335                 340                 345                         

cag ctc gtc gac gag gcg ttc cag cgg tac cac ggc gcg gcg cgg gag     2066
Gln Leu Val Asp Glu Ala Phe Gln Arg Tyr His Gly Ala Ala Arg Glu         
350                 355                 360                 365         

tcg aag ttg tag ggcgggattc ctgcaggagc tcgaatttcc ccgatcgttc         2118
Ser Lys Leu                                                             
                                                                        

aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat   2178

catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt   2238

atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga   2298

aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact   2358

agatcgggaa ttccttaatt aatctagagg cgcgccgggc ccggccggcc agatcttgat   2418

tgtcgtttcc cgccttcagt ttaaactatc agtgtttgac aggatatatt ggcgggtaaa   2478

cctaagagaa aagagcgttt attagaataa tcggatattt aaaagggcgt gaaaaggttt   2538

atccgttcgt ccatttgtat gt                                            2560


<210>  18
<211>  368
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  18

Met His Ser Gln Lys Arg Val Val Val Leu Gly Ser Gly Val Ile Gly 
1               5                   10                  15      


Leu Ser Ser Ala Leu Ile Leu Ala Arg Lys Gly Tyr Ser Val His Ile 
            20                  25                  30          


Leu Ala Arg Asp Leu Pro Glu Asp Val Ser Ser Gln Thr Phe Ala Ser 
        35                  40                  45              


Pro Trp Ala Gly Ala Asn Trp Thr Pro Phe Met Thr Leu Thr Asp Gly 
    50                  55                  60                  


Pro Arg Gln Ala Lys Trp Glu Glu Ser Thr Phe Lys Lys Trp Val Glu 
65                  70                  75                  80  


Leu Val Pro Thr Gly His Ala Met Trp Leu Lys Gly Thr Arg Arg Phe 
                85                  90                  95      


Ala Gln Asn Glu Asp Gly Leu Leu Gly His Trp Tyr Lys Asp Ile Thr 
            100                 105                 110         


Pro Asn Tyr Arg Pro Leu Pro Ser Ser Glu Cys Pro Pro Gly Ala Ile 
        115                 120                 125             


Gly Val Thr Tyr Asp Thr Leu Ser Val His Ala Pro Lys Tyr Cys Gln 
    130                 135                 140                 


Tyr Leu Ala Arg Glu Leu Gln Lys Leu Gly Ala Thr Phe Glu Arg Arg 
145                 150                 155                 160 


Thr Val Thr Ser Leu Glu Gln Ala Phe Asp Gly Ala Asp Leu Val Val 
                165                 170                 175     


Asn Ala Thr Gly Leu Gly Ala Lys Ser Ile Ala Gly Ile Asp Asp Gln 
            180                 185                 190         


Ala Ala Glu Pro Ile Arg Gly Gln Thr Val Leu Val Lys Ser Pro Cys 
        195                 200                 205             


Lys Arg Cys Thr Met Asp Ser Ser Asp Pro Ala Ser Pro Ala Tyr Ile 
    210                 215                 220                 


Ile Pro Arg Pro Gly Gly Glu Val Ile Cys Gly Gly Thr Tyr Gly Val 
225                 230                 235                 240 


Gly Asp Trp Asp Leu Ser Val Asn Pro Glu Thr Val Gln Arg Ile Leu 
                245                 250                 255     


Lys His Cys Leu Arg Leu Asp Pro Thr Ile Ser Ser Asp Gly Thr Ile 
            260                 265                 270         


Glu Gly Ile Glu Val Leu Arg His Asn Val Gly Leu Arg Pro Ala Arg 
        275                 280                 285             


Arg Gly Gly Pro Arg Val Glu Ala Glu Arg Ile Val Leu Pro Leu Asp 
    290                 295                 300                 


Arg Thr Lys Ser Pro Leu Ser Leu Gly Arg Gly Ser Ala Arg Ala Ala 
305                 310                 315                 320 


Lys Glu Lys Glu Val Thr Leu Val His Ala Tyr Gly Phe Ser Ser Ala 
                325                 330                 335     


Gly Tyr Gln Gln Ser Trp Gly Ala Ala Glu Asp Val Ala Gln Leu Val 
            340                 345                 350         


Asp Glu Ala Phe Gln Arg Tyr His Gly Ala Ala Arg Glu Ser Lys Leu 
        355                 360                 365             


<210>  19
<211>  3411
<212>  DNA
<213>  Artificial

<220>
<223>  T-DNA region from RLM240, a RLM220-type binary vector containing 
       a p-ScBV::c-dsdA::t-NOS selection cassette.


<220>
<221>  misc_structure
<222>  (1)..(128)
<223>  Left T-DNA border from pSB11

<220>
<221>  misc_binding
<222>  (47)..(71)
<223>  Left T-DNA border repeat from pSB11

<220>
<221>  promoter
<222>  (154)..(1551)
<223>  Sugarcane bacilliform virus [ScBV] promoter region

<220>
<221>  CDS
<222>  (1588)..(2916)
<223>  E.coli D-serine dehydratase [dsdA] gene/CDS

<220>
<221>  terminator
<222>  (2962)..(3214)
<223>  Agrobacterium tumefaciens nopaline synthase terminator region

<220>
<221>  misc_structure
<222>  (3267)..(3411)
<223>  Right T-DNA border from pSB11

<220>
<221>  misc_binding
<222>  (3307)..(3330)
<223>  Right T-DNA border repeat from pSB11

<400>  19
gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt     60

gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt    120

actgaattgg atccgcccgg gcggtacatc ttaaatcctg gctagcaaca ctgaactatg    180

ccagaaacca catcaaagat atgggcaagc ttcttggccc attatatcca aagacctcag    240

agaaaggtga gcgaaggctc aattcagaag attggaagct gatcaatagg atcaagacaa    300

tggtgagaac gcttccaaat ctcactattc caccagaaga tgcatacatt atcattgaaa    360

cagatgcatg tgcaactgga tggggagcag tatgcaagtg gaagaaaaac aaggcagacc    420

caagaaatac agagcaaatc tgtaggtatg ccagtggaaa atttgataag ccaaaaggaa    480

cctgtgatgc agaaatctat ggggttatga atggcttaga aaagatgaga ttgttctact    540

tggacaaaag agagatcaca gtcagaactg acagtagtgc aatcgaaagg ttctacaaca    600

agagtgctga acacaagcct tctgagatca gatggatcag gttcatggac tacatcactg    660

gtgcaggacc agagatagtc attgaacaca taaaagggaa gagcaatggt ttagctgaca    720

tcttgtccag gctcaaagcc aaattagctc agaatgaacc aacggaagag atgatcctgc    780

ttacacaagc cataagggaa gtaattcctt atccagatca tccatacact gagcaactca    840

gagaatgggg aaacaaaatt ctggatccat tccccacatt caagaaggac atgttcgaaa    900

gaacagagca agcttttatg ctaacagagg aaccagttct actctgtgca tgcaggaagc    960

ctgcaattca gttagtgtcc agaacatctg ccaacccagg aaggaaattc ttcaagtgcg   1020

caatgaacaa atgccattgc tggtactggg cagatctcat tgaagaacac attcaagaca   1080

gaattgatga atttctcaag aatcttgaag ttctgaagac cggtggcgtg caaacaatgg   1140

aggaggaact tatgaaggaa gtcaccaagc tgaagataga agagcaggag ttcgaggaat   1200

accaggccac accaagggct atgtcgccag tagccgcaga agatgtgcta gatctccaag   1260

acgtaagcaa tgacgattga ggaggcattg acgtcaggga tgaccgcagc ggagagtact   1320

gggcccattc agtggatgct ccactgagtt gtattattgt gtgcttttcg gacaagtgtg   1380

ctgtccactt tcttttggca cctgtgccac tttattcctt gtctgccacg atgcctttgc   1440

ttagcttgta agcaaggatc gcagtgcgtg tgtgacacca ccccccttcc gacgctctgc   1500

ctatataagg caccgtctgt aagctcttac gatcatcggt agttcaccaa gctcgaggat   1560

cctcatctaa gcgcaaagag acgtact atg gaa aac gct aaa atg aac tcg ctc   1614
                              Met Glu Asn Ala Lys Met Asn Ser Leu       
                              1               5                         

atc gcc cag tat ccg ttg gta aag gat ctg gtt gct ctt aaa gaa acc     1662
Ile Ala Gln Tyr Pro Leu Val Lys Asp Leu Val Ala Leu Lys Glu Thr         
10                  15                  20                  25          

acc tgg ttt aat cct ggc acg acc tca ttg gct gaa ggt tta cct tat     1710
Thr Trp Phe Asn Pro Gly Thr Thr Ser Leu Ala Glu Gly Leu Pro Tyr         
                30                  35                  40              

gtt ggc ctg acc gaa cag gat gtt cag gac gcc cat gcg cgc tta tcc     1758
Val Gly Leu Thr Glu Gln Asp Val Gln Asp Ala His Ala Arg Leu Ser         
            45                  50                  55                  

cgt ttt gca ccc tat ctg gca aaa gca ttt cct gaa act gct gcc act     1806
Arg Phe Ala Pro Tyr Leu Ala Lys Ala Phe Pro Glu Thr Ala Ala Thr         
        60                  65                  70                      

ggg ggg att att gaa tca gaa ctg gtt gcc att cca gct atg caa aaa     1854
Gly Gly Ile Ile Glu Ser Glu Leu Val Ala Ile Pro Ala Met Gln Lys         
    75                  80                  85                          

cgg ctg gaa aaa gaa tat cag caa ccg atc agc ggg caa ctg tta ctg     1902
Arg Leu Glu Lys Glu Tyr Gln Gln Pro Ile Ser Gly Gln Leu Leu Leu         
90                  95                  100                 105         

aaa aaa gat agc cat ttg ccc att tcc ggc tcc ata aaa gca cgc ggc     1950
Lys Lys Asp Ser His Leu Pro Ile Ser Gly Ser Ile Lys Ala Arg Gly         
                110                 115                 120             

ggg att tat gaa gtc ctg gca cac gca gaa aaa ctg gct ctg gaa gcg     1998
Gly Ile Tyr Glu Val Leu Ala His Ala Glu Lys Leu Ala Leu Glu Ala         
            125                 130                 135                 

ggg ttg ctg acg ctt gat gat gac tac agc aaa ctg ctt tct ccg gag     2046
Gly Leu Leu Thr Leu Asp Asp Asp Tyr Ser Lys Leu Leu Ser Pro Glu         
        140                 145                 150                     

ttt aaa cag ttc ttt agc caa tac agc att gct gtg ggc tca acc gga     2094
Phe Lys Gln Phe Phe Ser Gln Tyr Ser Ile Ala Val Gly Ser Thr Gly         
    155                 160                 165                         

aat ctg ggg tta tca atc ggc att atg agc gcc cgc att ggc ttt aag     2142
Asn Leu Gly Leu Ser Ile Gly Ile Met Ser Ala Arg Ile Gly Phe Lys         
170                 175                 180                 185         

gtg aca gtt cat atg tct gct gat gcc cgg gca tgg aaa aaa gcg aaa     2190
Val Thr Val His Met Ser Ala Asp Ala Arg Ala Trp Lys Lys Ala Lys         
                190                 195                 200             

ctg cgc agc cat ggc gtt acg gtc gtg gaa tat gag caa gat tat ggt     2238
Leu Arg Ser His Gly Val Thr Val Val Glu Tyr Glu Gln Asp Tyr Gly         
            205                 210                 215                 

gtt gcc gtc gag gaa gga cgt aaa gca gcg cag tct gac ccg aac tgt     2286
Val Ala Val Glu Glu Gly Arg Lys Ala Ala Gln Ser Asp Pro Asn Cys         
        220                 225                 230                     

ttc ttt att gat gac gaa aat tcc cgc acg ttg ttc ctt ggg tat tcc     2334
Phe Phe Ile Asp Asp Glu Asn Ser Arg Thr Leu Phe Leu Gly Tyr Ser         
    235                 240                 245                         

gtc gct ggc cag cgt ctt aaa gcg caa ttt gcc cag caa ggc cgt atc     2382
Val Ala Gly Gln Arg Leu Lys Ala Gln Phe Ala Gln Gln Gly Arg Ile         
250                 255                 260                 265         

gtc gat gct gat aac cct ctg ttt gtc tat ctg ccg tgt ggt gtt ggc     2430
Val Asp Ala Asp Asn Pro Leu Phe Val Tyr Leu Pro Cys Gly Val Gly         
                270                 275                 280             

ggt ggt cct ggt ggc gtc gca ttc ggg ctt aaa ctg gcg ttt ggc gat     2478
Gly Gly Pro Gly Gly Val Ala Phe Gly Leu Lys Leu Ala Phe Gly Asp         
            285                 290                 295                 

cat gtt cac tgc ttt ttt gcc gaa cca acg cac tcc cct tgt atg ttg     2526
His Val His Cys Phe Phe Ala Glu Pro Thr His Ser Pro Cys Met Leu         
        300                 305                 310                     

tta ggc gtc cat aca gga tta cac gat cag att tct gtt cag gat att     2574
Leu Gly Val His Thr Gly Leu His Asp Gln Ile Ser Val Gln Asp Ile         
    315                 320                 325                         

ggt atc gac aac ctt acc gca gcg gat ggc ctt gca gtt ggt cgc gca     2622
Gly Ile Asp Asn Leu Thr Ala Ala Asp Gly Leu Ala Val Gly Arg Ala         
330                 335                 340                 345         

tca ggc ttt gtc ggg cgg gca atg gag cgt ctg ctg gat ggc ttc tat     2670
Ser Gly Phe Val Gly Arg Ala Met Glu Arg Leu Leu Asp Gly Phe Tyr         
                350                 355                 360             

acc ctt agc gat caa acc atg tat gac atg ctt ggc tgg ctg gcg cag     2718
Thr Leu Ser Asp Gln Thr Met Tyr Asp Met Leu Gly Trp Leu Ala Gln         
            365                 370                 375                 

gaa gaa ggt att cgt ctt gaa cct tcg gca ctg gcg ggt atg gcc gga     2766
Glu Glu Gly Ile Arg Leu Glu Pro Ser Ala Leu Ala Gly Met Ala Gly         
        380                 385                 390                     

cct cag cgc gtg tgt gca tca gta agt tac caa cag atg cac ggt ttc     2814
Pro Gln Arg Val Cys Ala Ser Val Ser Tyr Gln Gln Met His Gly Phe         
    395                 400                 405                         

agc gca gaa caa ctg cgt aat acc act cat ctg gtg tgg gcg acg gga     2862
Ser Ala Glu Gln Leu Arg Asn Thr Thr His Leu Val Trp Ala Thr Gly         
410                 415                 420                 425         

ggt gga atg gtg ccg gaa gaa gag atg aat caa tat ctg gca aaa ggc     2910
Gly Gly Met Val Pro Glu Glu Glu Met Asn Gln Tyr Leu Ala Lys Gly         
                430                 435                 440             

cgt taa taacgtttca acgcagcatg gatcgtaccg agctcgaatt tccccgatcg      2966
Arg                                                                     
                                                                        

ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat   3026

tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac   3086

gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat   3146

agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt   3206

actagatcgg gaattcctta attaatctag aggcgcgccg ggcccggccg gccagatctt   3266

gattgtcgtt tcccgccttc agtttaaact atcagtgttt gacaggatat attggcgggt   3326

aaacctaaga gaaaagagcg tttattagaa taatcggata tttaaaaggg cgtgaaaagg   3386

tttatccgtt cgtccatttg tatgt                                         3411


<210>  20
<211>  442
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  20

Met Glu Asn Ala Lys Met Asn Ser Leu Ile Ala Gln Tyr Pro Leu Val 
1               5                   10                  15      


Lys Asp Leu Val Ala Leu Lys Glu Thr Thr Trp Phe Asn Pro Gly Thr 
            20                  25                  30          


Thr Ser Leu Ala Glu Gly Leu Pro Tyr Val Gly Leu Thr Glu Gln Asp 
        35                  40                  45              


Val Gln Asp Ala His Ala Arg Leu Ser Arg Phe Ala Pro Tyr Leu Ala 
    50                  55                  60                  


Lys Ala Phe Pro Glu Thr Ala Ala Thr Gly Gly Ile Ile Glu Ser Glu 
65                  70                  75                  80  


Leu Val Ala Ile Pro Ala Met Gln Lys Arg Leu Glu Lys Glu Tyr Gln 
                85                  90                  95      


Gln Pro Ile Ser Gly Gln Leu Leu Leu Lys Lys Asp Ser His Leu Pro 
            100                 105                 110         


Ile Ser Gly Ser Ile Lys Ala Arg Gly Gly Ile Tyr Glu Val Leu Ala 
        115                 120                 125             


His Ala Glu Lys Leu Ala Leu Glu Ala Gly Leu Leu Thr Leu Asp Asp 
    130                 135                 140                 


Asp Tyr Ser Lys Leu Leu Ser Pro Glu Phe Lys Gln Phe Phe Ser Gln 
145                 150                 155                 160 


Tyr Ser Ile Ala Val Gly Ser Thr Gly Asn Leu Gly Leu Ser Ile Gly 
                165                 170                 175     


Ile Met Ser Ala Arg Ile Gly Phe Lys Val Thr Val His Met Ser Ala 
            180                 185                 190         


Asp Ala Arg Ala Trp Lys Lys Ala Lys Leu Arg Ser His Gly Val Thr 
        195                 200                 205             


Val Val Glu Tyr Glu Gln Asp Tyr Gly Val Ala Val Glu Glu Gly Arg 
    210                 215                 220                 


Lys Ala Ala Gln Ser Asp Pro Asn Cys Phe Phe Ile Asp Asp Glu Asn 
225                 230                 235                 240 


Ser Arg Thr Leu Phe Leu Gly Tyr Ser Val Ala Gly Gln Arg Leu Lys 
                245                 250                 255     


Ala Gln Phe Ala Gln Gln Gly Arg Ile Val Asp Ala Asp Asn Pro Leu 
            260                 265                 270         


Phe Val Tyr Leu Pro Cys Gly Val Gly Gly Gly Pro Gly Gly Val Ala 
        275                 280                 285             


Phe Gly Leu Lys Leu Ala Phe Gly Asp His Val His Cys Phe Phe Ala 
    290                 295                 300                 


Glu Pro Thr His Ser Pro Cys Met Leu Leu Gly Val His Thr Gly Leu 
305                 310                 315                 320 


His Asp Gln Ile Ser Val Gln Asp Ile Gly Ile Asp Asn Leu Thr Ala 
                325                 330                 335     


Ala Asp Gly Leu Ala Val Gly Arg Ala Ser Gly Phe Val Gly Arg Ala 
            340                 345                 350         


Met Glu Arg Leu Leu Asp Gly Phe Tyr Thr Leu Ser Asp Gln Thr Met 
        355                 360                 365             


Tyr Asp Met Leu Gly Trp Leu Ala Gln Glu Glu Gly Ile Arg Leu Glu 
    370                 375                 380                 


Pro Ser Ala Leu Ala Gly Met Ala Gly Pro Gln Arg Val Cys Ala Ser 
385                 390                 395                 400 


Val Ser Tyr Gln Gln Met His Gly Phe Ser Ala Glu Gln Leu Arg Asn 
                405                 410                 415     


Thr Thr His Leu Val Trp Ala Thr Gly Gly Gly Met Val Pro Glu Glu 
            420                 425                 430         


Glu Met Asn Gln Tyr Leu Ala Lys Gly Arg 
        435                 440         


<210>  21
<211>  2811
<212>  DNA
<213>  Artificial

<220>
<223>  T-DNA region from RLM239, a RLM220-type binary vector containing 
       a p-ZmAHASL::c-dsdA::t-NOS selection cassette.


<220>
<221>  misc_structure
<222>  (1)..(128)
<223>  Left T-DNA border from pSB11

<220>
<221>  misc_binding
<222>  (47)..(71)
<223>  Left T-DNA border repeat from pSB11

<220>
<221>  promoter
<222>  (153)..(952)
<223>  AHASL2 [XI12] promoter and 5'UTR regions from Z. mays 
       acetohydroxyacid synthase (AHAS108) gene

<220>
<221>  CDS
<222>  (988)..(2316)
<223>  E.coli D-serine dehydratase [dsdA] gene/CDS

<220>
<221>  terminator
<222>  (2362)..(2614)
<223>  Agrobacterium tumefaciens nopaline synthase terminator region

<220>
<221>  misc_structure
<222>  (2667)..(2811)
<223>  Right T-DNA border from pSB11

<220>
<221>  misc_binding
<222>  (2707)..(2730)
<223>  Right T-DNA border repeat from pSB11

<400>  21
gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt     60

gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt    120

actgaattgg atccgcccgg gcggtacatg caggtcaacg gatcacctat caacatccca    180

gctaaaaaca gtaaaaaggg ggaaaacgtg ggtgagttga gtctgtcttg tggaaaaaac    240

gttttagttt ctcctggaat taacaataaa aacagttgaa caagattgac tgttcctccg    300

ggagggtttg gaacatcgtt acagatgtga gcgaaaggtg aggaaacaga gcggagggct    360

tggaggtgac ctcggtagtc gacgccggag ttgagcctga tgacgacacc gtactggcgt    420

accaggccta gtagtgaaca ccgggcctga agctgtcgcc gccgctgctc atcttgtggg    480

ctgtgcccgg tgtccctgtt gcggattgcg ggtggcagcc tggcaggtgg gtgcgacccg    540

tttggactcc ctgatctggg ccctttgtgt cagtaccgtc tgtactccga tgacatgcac    600

accgtcgtcc acagtcaagt ccacaatctc ccctcttttt ttaacggaat agttcaaaat    660

ctccttgacg cacgctatcg tgtaccagcg ctcactggac accacgtttg taatccacgc    720

cgacacgtcg gtcccacgtc gacaggcccc accgtccggt ctgtagcgtg tacgtattcg    780

ggcgacggac gtgtcgtcgt cgtcttgcga gtcccattcc catcaccatc tgagccacac    840

atcctctgaa caaaagcagg gaggcctcta cgcacatccc cctttctccc actccgtgtc    900

cgtggcaccc accccaaacc ctcgcgccgc ctccgagaca gccgccgcaa cctcgaggat    960

cctcatctaa gcgcaaagag acgtact atg gaa aac gct aaa atg aac tcg ctc   1014
                              Met Glu Asn Ala Lys Met Asn Ser Leu       
                              1               5                         

atc gcc cag tat ccg ttg gta aag gat ctg gtt gct ctt aaa gaa acc     1062
Ile Ala Gln Tyr Pro Leu Val Lys Asp Leu Val Ala Leu Lys Glu Thr         
10                  15                  20                  25          

acc tgg ttt aat cct ggc acg acc tca ttg gct gaa ggt tta cct tat     1110
Thr Trp Phe Asn Pro Gly Thr Thr Ser Leu Ala Glu Gly Leu Pro Tyr         
                30                  35                  40              

gtt ggc ctg acc gaa cag gat gtt cag gac gcc cat gcg cgc tta tcc     1158
Val Gly Leu Thr Glu Gln Asp Val Gln Asp Ala His Ala Arg Leu Ser         
            45                  50                  55                  

cgt ttt gca ccc tat ctg gca aaa gca ttt cct gaa act gct gcc act     1206
Arg Phe Ala Pro Tyr Leu Ala Lys Ala Phe Pro Glu Thr Ala Ala Thr         
        60                  65                  70                      

ggg ggg att att gaa tca gaa ctg gtt gcc att cca gct atg caa aaa     1254
Gly Gly Ile Ile Glu Ser Glu Leu Val Ala Ile Pro Ala Met Gln Lys         
    75                  80                  85                          

cgg ctg gaa aaa gaa tat cag caa ccg atc agc ggg caa ctg tta ctg     1302
Arg Leu Glu Lys Glu Tyr Gln Gln Pro Ile Ser Gly Gln Leu Leu Leu         
90                  95                  100                 105         

aaa aaa gat agc cat ttg ccc att tcc ggc tcc ata aaa gca cgc ggc     1350
Lys Lys Asp Ser His Leu Pro Ile Ser Gly Ser Ile Lys Ala Arg Gly         
                110                 115                 120             

ggg att tat gaa gtc ctg gca cac gca gaa aaa ctg gct ctg gaa gcg     1398
Gly Ile Tyr Glu Val Leu Ala His Ala Glu Lys Leu Ala Leu Glu Ala         
            125                 130                 135                 

ggg ttg ctg acg ctt gat gat gac tac agc aaa ctg ctt tct ccg gag     1446
Gly Leu Leu Thr Leu Asp Asp Asp Tyr Ser Lys Leu Leu Ser Pro Glu         
        140                 145                 150                     

ttt aaa cag ttc ttt agc caa tac agc att gct gtg ggc tca acc gga     1494
Phe Lys Gln Phe Phe Ser Gln Tyr Ser Ile Ala Val Gly Ser Thr Gly         
    155                 160                 165                         

aat ctg ggg tta tca atc ggc att atg agc gcc cgc att ggc ttt aag     1542
Asn Leu Gly Leu Ser Ile Gly Ile Met Ser Ala Arg Ile Gly Phe Lys         
170                 175                 180                 185         

gtg aca gtt cat atg tct gct gat gcc cgg gca tgg aaa aaa gcg aaa     1590
Val Thr Val His Met Ser Ala Asp Ala Arg Ala Trp Lys Lys Ala Lys         
                190                 195                 200             

ctg cgc agc cat ggc gtt acg gtc gtg gaa tat gag caa gat tat ggt     1638
Leu Arg Ser His Gly Val Thr Val Val Glu Tyr Glu Gln Asp Tyr Gly         
            205                 210                 215                 

gtt gcc gtc gag gaa gga cgt aaa gca gcg cag tct gac ccg aac tgt     1686
Val Ala Val Glu Glu Gly Arg Lys Ala Ala Gln Ser Asp Pro Asn Cys         
        220                 225                 230                     

ttc ttt att gat gac gaa aat tcc cgc acg ttg ttc ctt ggg tat tcc     1734
Phe Phe Ile Asp Asp Glu Asn Ser Arg Thr Leu Phe Leu Gly Tyr Ser         
    235                 240                 245                         

gtc gct ggc cag cgt ctt aaa gcg caa ttt gcc cag caa ggc cgt atc     1782
Val Ala Gly Gln Arg Leu Lys Ala Gln Phe Ala Gln Gln Gly Arg Ile         
250                 255                 260                 265         

gtc gat gct gat aac cct ctg ttt gtc tat ctg ccg tgt ggt gtt ggc     1830
Val Asp Ala Asp Asn Pro Leu Phe Val Tyr Leu Pro Cys Gly Val Gly         
                270                 275                 280             

ggt ggt cct ggt ggc gtc gca ttc ggg ctt aaa ctg gcg ttt ggc gat     1878
Gly Gly Pro Gly Gly Val Ala Phe Gly Leu Lys Leu Ala Phe Gly Asp         
            285                 290                 295                 

cat gtt cac tgc ttt ttt gcc gaa cca acg cac tcc cct tgt atg ttg     1926
His Val His Cys Phe Phe Ala Glu Pro Thr His Ser Pro Cys Met Leu         
        300                 305                 310                     

tta ggc gtc cat aca gga tta cac gat cag att tct gtt cag gat att     1974
Leu Gly Val His Thr Gly Leu His Asp Gln Ile Ser Val Gln Asp Ile         
    315                 320                 325                         

ggt atc gac aac ctt acc gca gcg gat ggc ctt gca gtt ggt cgc gca     2022
Gly Ile Asp Asn Leu Thr Ala Ala Asp Gly Leu Ala Val Gly Arg Ala         
330                 335                 340                 345         

tca ggc ttt gtc ggg cgg gca atg gag cgt ctg ctg gat ggc ttc tat     2070
Ser Gly Phe Val Gly Arg Ala Met Glu Arg Leu Leu Asp Gly Phe Tyr         
                350                 355                 360             

acc ctt agc gat caa acc atg tat gac atg ctt ggc tgg ctg gcg cag     2118
Thr Leu Ser Asp Gln Thr Met Tyr Asp Met Leu Gly Trp Leu Ala Gln         
            365                 370                 375                 

gaa gaa ggt att cgt ctt gaa cct tcg gca ctg gcg ggt atg gcc gga     2166
Glu Glu Gly Ile Arg Leu Glu Pro Ser Ala Leu Ala Gly Met Ala Gly         
        380                 385                 390                     

cct cag cgc gtg tgt gca tca gta agt tac caa cag atg cac ggt ttc     2214
Pro Gln Arg Val Cys Ala Ser Val Ser Tyr Gln Gln Met His Gly Phe         
    395                 400                 405                         

agc gca gaa caa ctg cgt aat acc act cat ctg gtg tgg gcg acg gga     2262
Ser Ala Glu Gln Leu Arg Asn Thr Thr His Leu Val Trp Ala Thr Gly         
410                 415                 420                 425         

ggt gga atg gtg ccg gaa gaa gag atg aat caa tat ctg gca aaa ggc     2310
Gly Gly Met Val Pro Glu Glu Glu Met Asn Gln Tyr Leu Ala Lys Gly         
                430                 435                 440             

cgt taa taacgtttca acgcagcatg gatcgtaccg agctcgaatt tccccgatcg      2366
Arg                                                                     
                                                                        

ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat   2426

tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac   2486

gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat   2546

agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt   2606

actagatcgg gaattcctta attaatctag aggcgcgccg ggcccggccg gccagatctt   2666

gattgtcgtt tcccgccttc agtttaaact atcagtgttt gacaggatat attggcgggt   2726

aaacctaaga gaaaagagcg tttattagaa taatcggata tttaaaaggg cgtgaaaagg   2786

tttatccgtt cgtccatttg tatgt                                         2811


<210>  22
<211>  442
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  22

Met Glu Asn Ala Lys Met Asn Ser Leu Ile Ala Gln Tyr Pro Leu Val 
1               5                   10                  15      


Lys Asp Leu Val Ala Leu Lys Glu Thr Thr Trp Phe Asn Pro Gly Thr 
            20                  25                  30          


Thr Ser Leu Ala Glu Gly Leu Pro Tyr Val Gly Leu Thr Glu Gln Asp 
        35                  40                  45              


Val Gln Asp Ala His Ala Arg Leu Ser Arg Phe Ala Pro Tyr Leu Ala 
    50                  55                  60                  


Lys Ala Phe Pro Glu Thr Ala Ala Thr Gly Gly Ile Ile Glu Ser Glu 
65                  70                  75                  80  


Leu Val Ala Ile Pro Ala Met Gln Lys Arg Leu Glu Lys Glu Tyr Gln 
                85                  90                  95      


Gln Pro Ile Ser Gly Gln Leu Leu Leu Lys Lys Asp Ser His Leu Pro 
            100                 105                 110         


Ile Ser Gly Ser Ile Lys Ala Arg Gly Gly Ile Tyr Glu Val Leu Ala 
        115                 120                 125             


His Ala Glu Lys Leu Ala Leu Glu Ala Gly Leu Leu Thr Leu Asp Asp 
    130                 135                 140                 


Asp Tyr Ser Lys Leu Leu Ser Pro Glu Phe Lys Gln Phe Phe Ser Gln 
145                 150                 155                 160 


Tyr Ser Ile Ala Val Gly Ser Thr Gly Asn Leu Gly Leu Ser Ile Gly 
                165                 170                 175     


Ile Met Ser Ala Arg Ile Gly Phe Lys Val Thr Val His Met Ser Ala 
            180                 185                 190         


Asp Ala Arg Ala Trp Lys Lys Ala Lys Leu Arg Ser His Gly Val Thr 
        195                 200                 205             


Val Val Glu Tyr Glu Gln Asp Tyr Gly Val Ala Val Glu Glu Gly Arg 
    210                 215                 220                 


Lys Ala Ala Gln Ser Asp Pro Asn Cys Phe Phe Ile Asp Asp Glu Asn 
225                 230                 235                 240 


Ser Arg Thr Leu Phe Leu Gly Tyr Ser Val Ala Gly Gln Arg Leu Lys 
                245                 250                 255     


Ala Gln Phe Ala Gln Gln Gly Arg Ile Val Asp Ala Asp Asn Pro Leu 
            260                 265                 270         


Phe Val Tyr Leu Pro Cys Gly Val Gly Gly Gly Pro Gly Gly Val Ala 
        275                 280                 285             


Phe Gly Leu Lys Leu Ala Phe Gly Asp His Val His Cys Phe Phe Ala 
    290                 295                 300                 


Glu Pro Thr His Ser Pro Cys Met Leu Leu Gly Val His Thr Gly Leu 
305                 310                 315                 320 


His Asp Gln Ile Ser Val Gln Asp Ile Gly Ile Asp Asn Leu Thr Ala 
                325                 330                 335     


Ala Asp Gly Leu Ala Val Gly Arg Ala Ser Gly Phe Val Gly Arg Ala 
            340                 345                 350         


Met Glu Arg Leu Leu Asp Gly Phe Tyr Thr Leu Ser Asp Gln Thr Met 
        355                 360                 365             


Tyr Asp Met Leu Gly Trp Leu Ala Gln Glu Glu Gly Ile Arg Leu Glu 
    370                 375                 380                 


Pro Ser Ala Leu Ala Gly Met Ala Gly Pro Gln Arg Val Cys Ala Ser 
385                 390                 395                 400 


Val Ser Tyr Gln Gln Met His Gly Phe Ser Ala Glu Gln Leu Arg Asn 
                405                 410                 415     


Thr Thr His Leu Val Trp Ala Thr Gly Gly Gly Met Val Pro Glu Glu 
            420                 425                 430         


Glu Met Asn Gln Tyr Leu Ala Lys Gly Arg 
        435                 440         


